Comparison of carbohydrate-containing substances from non-calcified and calcified portions of bovine costal cartilage.
Carbohydrate-containing substances were extracted from non-calcified (NCC) and calcified (CC) portions of bovine costal cartilage with 0.5 M LaCl3 by the method of Mason and his co-workers, followed by dilution of the extract with 9 volumes of water. The precipitate formed on dilution yielded Fr. P, while Fr. S was obtained from the supernatant. Fr. P was separated into two subfractions by gel filtration on Sepharose 2B. The experimental results showed that Fr. P contained proteoglycans with different molecular sizes and compositions, while Fr. S contained proteoglycan, hyaluronic acid, glycoproteins, and glycogen. The present data suggest that in the proteoglycan of Fr. P, the relative content of chondroitin sulfate decreases with a concomitant increment in that of keratan sulfate on calcification. In addition, elevation of the ratio of chondroitin 4-sulfate to chondroitin 6-sulfate, together with a small increment of non-sulfated disaccharide units in the chondroitin sulfate chains appear to occur on calcification. The glycogen content in Fr. S diminished on calcification. The present observations suggest therefore that the remodeling of proteoglycan consumption of glycogen in bovine costal cartilage occur on calcification.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002356:Cartilage / Q000032:analysis*; D002417:Cattle; D002809:Chondroitin Sulfates / Q000032:analysis; D006003:Glycogen / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis
D002241:Carbohydrates; D006023:Glycoproteins; D006595:Hexosamines; D006601:Hexoses; D011509:Proteoglycans; D014574:Uronic Acids; D006820:Hyaluronic Acid; D006003:Glycogen; D002809:Chondroitin Sulfates