Sensitive GLC assay for pemoline in biological fluids using nitrogen-specific detection.
Extractive alkylation was used to determine intact pemoline in serum and urine. Pemoline was extracted into methylene chloride as an ion-pair with tetrapentylammonium hydroxide under alkaline conditions. Evaporation of the solvent at 70 degrees in the presence of methyl iodide yielded the N,N-dimethylpemoline derivative. GLC analysis was performed on a 5% FFAP column with nitrogen-specific detection. Sensitivity was 0.05 microgram/ml with 1 ml of urine of serum. Calibration curves were linear to at least 4 microgram/ml with serum and 15 microgram/ml with urine. Precision was excellent with a pooled relative standard deviation of +/- 7.5% for serum samples in a 0.1-4 microgram/ml range.
D002849:Chromatography, Gas; D006801:Humans; D008722:Methods; D009584:Nitrogen / Q000032:analysis; D010389:Pemoline / Q000032:analysis* / Q000097:blood / Q000652:urine
D010389:Pemoline; D009584:Nitrogen