title: Binding of N-methyl isatin beta-thiosemicarbazone-copper complexes to proteins and nucleic acids.
abstract: N-Methyl isatin beta-thiosemicarbazone-copper complexes interact with nucleic acids and proteins as shown by ultraviolet (UV) and visible spectroscopy and Sephadex exclusion chromatography. The Cu++ ions are most effective; Co++ ions have less albeit significant activity. Chelating agents, such as Tris and histidine, high NaCl concentration, and dimethyl sulfoxide reduce the binding of the drug-metal complex. The binding constant of the drug-copper complex to calf-thymus DNA was calculated to range between 6.9 x 10(4) and 2.7 x 10(5) M-1.
mesh_terms: D002614:Chelating Agents; D055598:Chemical Phenomena; D002621:Chemistry; D003300:Copper; D004247:DNA; D004121:Dimethyl Sulfoxide; D008720:Methisazone / Q000031:analogs & derivatives; D011485:Protein Binding; D011506:Proteins; D012329:RNA, Bacterial; D012965:Sodium Chloride; D013056:Spectrophotometry, Ultraviolet; D013882:Thiosemicarbazones / Q000031:analogs & derivatives
chemical_list: D002614:Chelating Agents; D011506:Proteins; D012329:RNA, Bacterial; D013882:Thiosemicarbazones; D012965:Sodium Chloride; D003300:Copper; D004247:DNA; D008720:Methisazone; D004121:Dimethyl Sulfoxide