[Comparative study of dehydrogenase and diaphorase activity in spinal ganglion neurons in vivo and during their cultivation in vitro].
Enzymatic activity was investigated in spinal ganglia of 10-day-old chick embryo and in 10-day-old cultures of these ganglia (the method of "flying glasses"). Histochemical preparations were photometrically treated with the one-wave plug-method in the device MCFB-1 (LOMO) with the diameter of the probe 8 mkm. As the result of the investigations on optic density, a definite conclusion was made about relative activity of 15 enzymes and a general scheme of neuronal metabolism was graphically presented under the conditions of cultivation, enzymatic activity of krebs cycle was demonstrated to decrease, while that of diaphorase and lactate dehydrogenase to increase. The data on topography of enzymatic distribution in neurons, satellites, lemmoncytes and fibroblasts were also presented.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology; D005727:Ganglia, Spinal / Q000166:cytology / Q000201:enzymology*; D006651:Histocytochemistry; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009457:Neuroglia / Q000201:enzymology; D009474:Neurons / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism*
D010088:Oxidoreductases; D009252:NADPH Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase
Stravnitel'noe izuchenie aktivnosti degidrogenaz i diaforaz v neĭronakh spinnomozgovogo uzla in vivo i pri kul'tivirovanii ikh in vitro.