Use of p-aminophenyl D and L-lactic acids and p-aminophenyl pyruvic acid as effectors in the affinity chromatography of lactate dehydrogenase.
p-Aminophenyl pyruvic acid and D-p-amino-phenyllactic acid were immobilized on a new synthetic acrylic carrier bearing acylating N-succinimidyl ester groups. The derivatives obtained were used successfully to purify lactate dehydrogenase (LDH) by affinity chromatography, the elution being carried out by means of NADH or preferably L-phenyllactic acid. Moreover, the specific activity of the LDH contained in a human blood serum was increased 270 times, using L-p-aminophenyllactic acid immobilized on a mixed polyacrylic agarose carrier.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D006801:Humans; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification*; D007773:Lactates; D007778:Lactobacillus / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D010667:Phenylpyruvic Acids; D011817:Rabbits
D007202:Indicators and Reagents; D007773:Lactates; D010667:Phenylpyruvic Acids; C020197:4-aminophenyllactic; D007770:L-Lactate Dehydrogenase