title: Free-flow electrophoresis for the separation of malaria infected and uninfected mouse erythrocytes and for the isolation of free parasites (Plasmodium vinckei): a new rapid technique for the liberation of malaria parasites from their host cells.
abstract: After aggregation of erythrocytes from malaria infected mice, the parasites (Plasmodium vinckei) could be set free using gentle mechanical forces. The mixture of freed parasites, infected and non-infected erythrocytes, and membraneous material was separated by free-flow electrophoresis. The free parasites produced were very pure and infectious. Morphological and enzymatic data on the separated fractions are presented. Free-flow electrophoresis also allowed the separation of infected and uninfected erythrocytes.
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D004586:Electrophoresis / Q000379:methods; D004903:Erythrocyte Aggregation; D004912:Erythrocytes / Q000469:parasitology*; D005260:Female; D008288:Malaria / Q000469:parasitology*; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D010961:Plasmodium / Q000302:isolation & purification*