Preparation and properties of polyethylene glycol-trypsin adducts.
The covalent attachment of polyethylene glycol of 5000 daltons to non-essential groups on trypsin produces an adduct that no longer precipitates with anti-trypsin antibody. In comparison with trypsin, polyethylene glycol-trypsin preparations show equal or greater activity against N-alpha-benzoyl-L-arginine ethyl ester, about one-fourth activity against angiotensin II, and little activity against bovine liver catalase. The polyethylene glycol-trypsin adduct dissolves soft blood clots at one-fourth the rate of trypsin. Soybean trypsin inhibitor produces two-thirds inhibition of the adduct under conditions that cause complete inhibition of trypsin.
D000804:Angiotensin II / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004789:Enzyme Activation; D005342:Fibrinolysis; D011092:Polyethylene Glycols / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014357:Trypsin / Q000276:immunology / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000494:pharmacology
D014361:Trypsin Inhibitors; D000804:Angiotensin II; D011092:Polyethylene Glycols; D002374:Catalase; D014357:Trypsin