Temporal organization of replication in DNA fibers of mammalian cells.
The extent of coordinate control over the multiple initiation events in DNA replication has been investigated in three mammalian cell lines by DNA fiber autoradiography. Quantitative estimates have been obtained of the degree of synchrony among initiations occurring on stretches of DNA. Synchrony decreases markedly with increasing distance between initiation sites in MDBK (bovine) and L929 (mouse) cells, but only slightly in muntjac cells. Possible control mechanisms for the initiation process are discussed.
D000818:Animals; D001345:Autoradiography; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D004261:DNA Replication; D003670:Deer; D051379:Mice; D013997:Time Factors