Carrier protein effects on DNA-cellulose chromatography of putative steroid receptors.
In the absence of carrier proteins, putative androgen receptors elute from DNA-cellulose in the range of 120 to 190 mM NaCl. However, in the presence of lysozyme, most of the receptor elutes in the range of 200 to 230 mM NaCl. This is the same range in which the lysozyme itself, a basic protein, elutes after being chromatographed in the same manner. Moreover, at low ionic strength, lysozyme also increases the sedimentation velocity of both androgen and estrogen receptors. In contrast, bovine serum albumin neither adheres to DNA-cellulose nor alters the sedimentation properties of these proteins. The lysozyme effects can account for some discrepancies reported in the literature. Thus, for qualitative elution studies, the use of lysozyme as a carrier protein is not advised, although its direct interaction with receptors might facilitate quantitative fractionation.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins; D002846:Chromatography, Affinity; D004247:DNA; D004958:Estradiol / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D051379:Mice; D009113:Muramidase; D011944:Receptors, Androgen / Q000302:isolation & purification* / Q000378:metabolism; D011987:Receptors, Steroid / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine; D013739:Testosterone / Q000378:metabolism
D002352:Carrier Proteins; D011944:Receptors, Androgen; D011987:Receptors, Steroid; D012710:Serum Albumin, Bovine; D013739:Testosterone; D004958:Estradiol; D004247:DNA; D009113:Muramidase