Immunoassay by differential pulse polarography.
An immunological method based on labeling an antigen with an electroactive group detectable by differential pulse polarography has been demonstrated. Estriol labeled with mercuric acetate is electroactive, giving a reduction wave at -300 millivolts versus a standard calomel electrode. Addition of estriol antibody to 4-mercuric acetate estriol diminishes the peak current as a result of the antigen-antibody binding reaction. Separation of free-labeled estriol from antibody-bound-labeled antigen is unnecessary. The method is potentially useful as an analytical immunological technique.
D004964:Estriol / Q000032:analysis; D007118:Immunoassay / Q000379:methods*; D009941:Organomercury Compounds; D011048:Polarography / Q000379:methods
D009941:Organomercury Compounds; D004964:Estriol