title: Repression of rRNA synthesis induced by disaggregation in Dictyostelium discoideum.
abstract: Cells disaggregated from the slugs of Dictyostelium discoideum continued to incorporate [3H]uridine or [3H]uracil, though at a lower rate than interphase amoebae. Disaggregation brought about a marked increase in the proportion of labeled poly(A)-containing RNA to labeled total RNA. The proportion reached the maximum in disaggregated cells after 1 h of incubation, but remained high even after 5 h of incubation. Reconstruction of slugs from the disaggregated cells did not bring about a decrease but a further increase in the proportion. The real proportion estimated from the minimum period of labeling reached almost 100% in the disaggregated cells, in contrast to about 30% in the interphase amoebae. The increase in the proportion is attributed to the disaggregation-induced repression of rRNA synthesis, which was supported by sucrose density gradient analyses of RNA synthesized in the disaggregated cells. The possibility that rRNA synthesis in this organism is regulated by the loss of cell contact is discussed.
mesh_terms: D002455:Cell Division; D004023:Dictyostelium / Q000502:physiology*; D008970:Molecular Weight; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D014498:Uracil / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
chemical_list: D012335:RNA, Ribosomal; D011061:Poly A; D014498:Uracil; D012313:RNA; D014529:Uridine