Quantitative measurement of intracellular RNA genomes of Rauscher murine leukemia virus by competition hybridization in DNA excess.
The technique of competition hybridization in DNA excess was used to determine the intracellular distribution of RNA genomes of Rauscher murine leukemia virus. An examination of subcellular RNA fractions revealed that 59% of intracellular viral RNA genomes were associated with the nuclear-enriched fraction, 41% with the cytoplasmic fraction, and 18% with the polysomal-enriched fraction. Also, an analysis of total cellular RNA disclosed that 20% of intracellular viral RNA genomes were polyadenylated and 80% were not polyadenylated.
D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D004279:DNA, Viral; D008722:Methods; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis; D011132:Polyribosomes / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D011925:Rauscher Virus / Q000032:analysis*; D013347:Subcellular Fractions
D004279:DNA, Viral; D012367:RNA, Viral; D011061:Poly A