Electron microscope autoradiography of a diffusible intracellular constituent, using freeze-dried frozen sections of mammalian intestinal epithelium.
Autoradiograms were prepared from freeze-dried frozen sections of rat intestinal epithelium, previously incubated in vitro with 3H-galactose. Although the sections showed evidence of disruption by ice-crystal growth during freezing, they retained sufficient recognizable ultrastructure to facilitate the identification of several subcellular compartments. Statistical analysis of the grain distribution indicated that a significant non-uniform distribution of the labelled galactose occurs in the absorptive cells during transport, and is maintained in the sections throughout the various stages of autoradiography.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D004058:Diffusion; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005612:Freeze Drying; D005629:Frozen Sections; D005690:Galactose / Q000378:metabolism*; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods*; D008867:Microtomy; D009940:Organoids / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000378:metabolism; D051381:Rats; D012834:Silver
D012834:Silver; D011092:Polyethylene Glycols; D005690:Galactose