Isolation of a sperm membrane sialoglycoprotein autoantigen from rabbit testes.
A single rabbit sperm membrane autoantigen has been isolated from rabbit testes by immunoadsorbent chromatography, agarose chromatography, and electrophoresis. This rabbit sperm autoantigen (RSA-1) is located in the sperm plasma membrane and blocks the sperm cytotoxic (immobilizing) activity of autoantisera. RSA-1 is a sialoglycoprotein, approximately 40% carbohydrate and containing 0.2 micrograms sialic acid/microgram protein. Its m.w. is estimated at 13,000 +/- 1200 by SDA-PAGE. RSA-1 can self-aggregate into higher m.w. forms. Approximately 0.6 mg of RSA-1 can be isolated from 100 rabbits testes. The isolated RSA-1 shows a 300-fold increase in specific cytotoxic (immobilizing) inhibitory activity over the starting testis pellet material.
D000818:Animals; D000941:Antigens; D000954:Antigens, Surface; D001323:Autoantibodies / Q000302:isolation & purification; D001324:Autoantigens; D002462:Cell Membrane / Q000276:immunology; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera / Q000494:pharmacology; D008297:Male; D011817:Rabbits; D012795:Sialoglycoproteins / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*; D013737:Testis / Q000276:immunology*
D000941:Antigens; D000954:Antigens, Surface; D001323:Autoantibodies; D001324:Autoantigens; D007106:Immune Sera; D012795:Sialoglycoproteins; D003165:Complement System Proteins