The mechanism of action of tRNA methylases studied with immobilized tRNAs.
Each of the individual tRNAs immobilized on aminohydroxybutyl-cellulose (ABC) through their oxidized 3'-terminal binds affinitively all methylases present in the enzyme extract irrespective of whether this tRNA will be involved in the following step of methylation or not. These data allow to suggest that (a) the formation of a methylase-tRNA complex and the catalytic act of methylation are indeed autonomous processes and (b) the first step of interaction between tRNAs and tRNA methylases is rather unspecific and consists in the recognition of the whole class of tRNA molecules.
D000818:Animals; D007680:Kidney Neoplasms / Q000201:enzymology; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D008745:Methylation; D009374:Neoplasms, Experimental / Q000201:enzymology; D012343:RNA, Transfer; D051381:Rats; D013379:Substrate Specificity; D012359:tRNA Methyltransferases / Q000378:metabolism*
D012343:RNA, Transfer; D012359:tRNA Methyltransferases