A precise quantitation of gene number by saturation hybridization using cloned DNA.
This paper describes a precise method of gene titration as applied to the alpha- and beta-globin genes in the mouse. The three salient features of the method are: (i) the use of saturation hybridization in probe cDNA excess, (ii) the use of highly purified cDNA probes prepared by preparative hybridization with cloned globin sequences (Longacre and Mach (1978) J. Biol. Chem. 253, 7500) and (iii) the use of cloned globin sequences to calibrate the system internally. The results indicate that there are two genes for alpha-globin and two genes for beta-globin in the BALB/c mouse. The significance of these results are discussed in relation to other data regarding adult and embryonic globin genes.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004274:DNA, Recombinant / Q000378:metabolism; D004622:Embryo, Mammalian; D005796:Genes; D005815:Genetic Code; D005914:Globins / Q000096:biosynthesis; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D004274:DNA, Recombinant; D005914:Globins; D004247:DNA