General transduction in Rhizobium meliloti by a thermosensitive mutant of bacteriophage DF2.
A simiplified version of bacteriophage DF2 transduction, using the thermosensitive mutant DF2tsC, is described. The transduction frequencies of Rhizobium meliloti markers by DF1tsC are of about 10(-6) or higher. The development of transductants at 34 degrees C does not require the addition of antiserum for phage neutralization.
D001435:Bacteriophages / Q000235:genetics*; D009154:Mutation; D012231:Rhizobium / Q000235:genetics*; D013696:Temperature; D014161:Transduction, Genetic