Analysis of lidocaine and its dealkylated metabolites by high-pressure liquid chromatography.
A high-pressure liquid chromatographic method is presented for determining lidocaine and its dealkylated metabolites, monoethylglycinexylidide and glycinexylidide, in plasma. This method uses 20-microliter samples injected directly through a pre-column onto a C18 analytical column. It is fast, sensitive and linear over a concentration range of 1--10 microgram/ml. Since the method requires no preliminary treatment of the plasma, it provides a practical means of monitoring lidocaine and its metabolites in therapeutic situations.
D000478:Alkylation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008012:Lidocaine / Q000097:blood* / Q000378:metabolism / Q000627:therapeutic use
D008012:Lidocaine