Extraction of small amounts of oxytocin from biological fluids by means of agarose-bound neurophysin.
The use of agarose-bound neurophysin for the extraction of oxytocin from biological fluids is described. Oxytocin can be extracted from plasma, urine and cerebrospinal fluid with a high rate of recovery and samples varying widely in volume and oxytocin concentration can be tested by the method. Columns can be used to extract and concentrate dilute samples, or to help identify small amounts of neurohypophysial hormones by affinity chromatography. The oxytocin can be eluted from the column directly into the buffer used for subsequent bioassay. The composition of the final extract is constant and independent of the composition of the sample. The specificity of the binding is high. It is suggested that the method has many advantages over others in current use.
D000818:Animals; D002847:Chromatography, Agarose / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008321:Mammary Glands, Animal / Q000032:analysis; D051379:Mice; D009481:Neurophysins / Q000378:metabolism; D010121:Oxytocin / Q000097:blood / Q000134:cerebrospinal fluid / Q000302:isolation & purification* / Q000652:urine
D009481:Neurophysins; D010121:Oxytocin