title: Combined platelet-leukapheresis: a technique for preparing separate platelet and granulocyte-platelet units from single donors.
abstract: One donor can provide separate platelet and granulocyte-platelet units that have the potential to fulfill the needs of two recipients. Combined platelet and leukapheresis were performed in the presence of hyddroxyethyl starch and citrate employing the Haemonetics Model 30 Blood Separator. Platelets and granulocytes were separated subsequently by centrifugation and two individual units were prepared for transfusion. The platelet units contained a mean of 4.4 x 10(11) platelets/unit and were nearly devoid of leukocytes. The combined granulocyte-platelet units contained a mean of 7.38 x 10(9) neutrophils/unit and 3.06 x 10(11) platelets/unit. Thus two separate units containing sufficient numbers of cells for transfusion can be obtained from pheresis of one donor.
mesh_terms: D001782:Blood Donors; D001803:Blood Transfusion; D006098:Granulocytes; D006801:Humans; D007937:Leukapheresis; D010983:Plateletpheresis