A new method for producing virus plaques in cell cultures.
Human oesophageal carcinoma cells can be grown on one side of a microporous gelatin membrane and fed from the other side. When they are infected with human herpesvirus type I at high dilutions, they produce plaques of virus-infected cells which can be detected by ordinary staining methods. This procedure may be suitable for the determination of the numbers of infectious particles in preparations of other viruses which are difficult to assay by conventional plaque assay techniques.
D010948:Viral Plaque Assay / Q000295:instrumentation / Q000379:methods*