title: Immunoassay by differential pulse polarography.
abstract: An immunological method based on labeling an antigen with an electroactive group detectable by differential pulse polarography has been demonstrated. Estriol labeled with mercuric acetate is electroactive, giving a reduction wave at -300 millivolts versus a standard calomel electrode. Addition of estriol antibody to 4-mercuric acetate estriol diminishes the peak current as a result of the antigen-antibody binding reaction. Separation of free-labeled estriol from antibody-bound-labeled antigen is unnecessary. The method is potentially useful as an analytical immunological technique.
mesh_terms: D004964:Estriol / Q000032:analysis; D007118:Immunoassay / Q000379:methods*; D009941:Organomercury Compounds; D011048:Polarography / Q000379:methods
chemical_list: D009941:Organomercury Compounds; D004964:Estriol