Interfamilial transfer of amber suppressor gene for the isolation of amber mutants of Mycobacteriophage I3.
A suppressor-containing strain of Mycobacterium smegmatis SN2 was isolated by transferring an amber suppressor carried on the plasmid of Pseudomonas pseudoalcaligenes ERA through transformation. Amber mutants of mycobacteriophage I3 were isolated.
D004352:Drug Resistance, Microbial; D009154:Mutation; D009160:Mycobacteriophages / Q000235:genetics / Q000302:isolation & purification*; D009161:Mycobacterium / Q000187:drug effects / Q000235:genetics*; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics*; D013489:Suppression, Genetic; D013752:Tetracycline / Q000494:pharmacology; D014169:Transformation, Bacterial
D013752:Tetracycline