Phenotyping of eight erythrocytic enzymes in one acrylamide gel.
A procedure has been developed to phenotype eight erythrocytic enzymes, phosphoglucomutase (PGM1), adenylate kinase (AK), 6-phosphogluconate dehydrogenase (6PGD), adenosine deaminase (ADA), glyoxalase (GLO), esterase-D (EsD), acid phosphatase (AcP), and glutamic pyruvate transaminase (GPT) in one acrylamide gel and also to detect the presence of common abnormal hemoglobins. The agar overlay technic has been eliminated. This simplified procedure renders the phenotyping of erythrocytic enzymes practical in paternity testing.
D000135:Acid Phosphatase / Q000097:blood; D000243:Adenosine Deaminase / Q000097:blood; D000263:Adenylate Kinase / Q000097:blood; D000410:Alanine Transaminase / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology*; D004950:Esterases / Q000097:blood; D006455:Hemoglobins, Abnormal / Q000032:analysis; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000097:blood; D010334:Paternity; D010641:Phenotype; D010733:Phosphoglucomutase / Q000097:blood; D010734:Phosphogluconate Dehydrogenase / Q000097:blood
D006455:Hemoglobins, Abnormal; D010734:Phosphogluconate Dehydrogenase; D000410:Alanine Transaminase; D000263:Adenylate Kinase; D004950:Esterases; D000135:Acid Phosphatase; D000243:Adenosine Deaminase; D007791:Lactoylglutathione Lyase; D010733:Phosphoglucomutase