title: Promotion of epithelial keratinization by N-methyl-N'-nitro-N-nitrosoguanidine in rat forestomach in organ culture.
abstract: The effect of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) on epithelial differentiation of fetal rat forestomach was investigated in organ culture. When forestomach tissues removed from 16.5-day fetuses were treated with 5 microgram and 3 microgram of MNNG per ml for 1 h, epithelial keratinization was observed after 4 and 5 days, respectively, whereas it occurred after 6 days in control cultures. A clear dose-response relationship was found in the promotion of epithelial keratinization by MNNG.
mesh_terms: D000818:Animals; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009924:Organ Culture Techniques; D011247:Pregnancy; D051381:Rats; D013270:Stomach / Q000187:drug effects; D013997:Time Factors
chemical_list: D008769:Methylnitronitrosoguanidine; D007633:Keratins