title: Activation of Sepharose with epichlorohydrin and subsequent immobilization of ligand for affinity adsorbent.
abstract: The optimal conditions for the activation of Sepharose by epichlorohydrin and subsequent immobilization of ligands were investigated. Under the optimal conditions for activation, namely, 30% Sepharose-5% epichlorohydrin-0.4 M NaOH, 40 degrees C, 2 h, the maximum amount of epoxy group was introduced into Sepharose with low cross-linking. The absorbents obtained by using N-acetyl-D-glucosamine, tri-N-acetylchitotriose, and glycoprotein as a ligand exhibited no nonspecific adsorption and good permeability for the high molecular substance to be purified, and were stable in an alkaline solution. Solanum tuberosum agglutinin was specifically adsorbed on a tri-N-acetylchitotriose-Sepharose column and was quantitatively recovered by elution with 0.2 M ammonia solution. Furthermore, the column could be repeatedly used under these conditions without reduction of its capacity.
mesh_terms: D000117:Acetylglucosamine; D002241:Carbohydrates; D002728:Chlorohydrins; D002846:Chromatography, Affinity; D004811:Epichlorohydrin; D006386:Hemagglutination Tests; D008024:Ligands; D011134:Polysaccharides; D012685:Sepharose; D013954:Thyroglobulin
chemical_list: D002241:Carbohydrates; D002728:Chlorohydrins; D008024:Ligands; D011134:Polysaccharides; D004811:Epichlorohydrin; D013954:Thyroglobulin; D012685:Sepharose; D000117:Acetylglucosamine