title: Immunochemical determination of two trypsins in human duodenal juice.
abstract: An application of electroimmunoassay to the separate determination of anionic and cationic trypsin in human duodenal juice is presented. The proportions of anionic to cationic immunoactive trypsin in duodenal juice from a group of children averaged 20 : 80. The ratio of immunoactive to esterolytically (BAPNA) active trypsin averaged 1.6 : 1, indicating the presence of inactive forms of trypsin in duodenal juice.
mesh_terms: D002648:Child; D002675:Child, Preschool; D004386:Duodenum / Q000201:enzymology*; D004789:Enzyme Activation; D005767:Gastrointestinal Diseases / Q000201:enzymology; D006801:Humans; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007223:Infant; D007527:Isoenzymes / Q000032:analysis*; D014357:Trypsin / Q000032:analysis*
chemical_list: D007527:Isoenzymes; D014357:Trypsin