Metabolic and secretory processes in nerve-endings isolated from post-mortem brain.
Isolated nerve ending particles (synaptosomes) prepared from rat and human cerebral cortex, after varying conditions of post-mortem storage were shown to possess good structural and morphological integrity and metabolic activity. Respiration, potassium retention, lactate dehydrogenase content, and stimulus-induced release of transmitter candidate amino acids, as well as the neuropeptide somatostatin, were measured. These preparations from post-mortem material showed properties which were closely comparable with similar preparations from fresh material. The relevance of these findings to studies on human post-mortem material is discussed.
D000368:Aged; D000596:Amino Acids; D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology / Q000378:metabolism*; D003080:Cold Temperature; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010101:Oxygen Consumption; D011180:Postmortem Changes; D011188:Potassium / Q000378:metabolism; D051381:Rats; D013004:Somatostatin / Q000378:metabolism; D013574:Synaptosomes / Q000201:enzymology / Q000378:metabolism; D014021:Tissue Preservation
D000596:Amino Acids; D013004:Somatostatin; D007770:L-Lactate Dehydrogenase; D011188:Potassium