Influence of perfusion and mechanical activity of ornithine decarboxylase activity in the isolated rat heart.
A small, but not significant, decrease in ornithine decarboxylase activity occurred in isolated hearts during one hour of recirculating perfusion with Krebs-Henseleit solution. A significantly greater decrease occurred with a non-recirculating system. Ornithine decarboxylase activity was the same in the working heart preparation as in the non-working preparation. It is concluded that a substance, necessary for the maintenance of ornithine decarboxylase, is removed during perfusion. The nature of the mechanical activity of the heart did not appear to be a determining factor.
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D066298:In Vitro Techniques; D009200:Myocardial Contraction; D009206:Myocardium / Q000201:enzymology*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D013997:Time Factors
D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase