Nucleosome cores reconstituted from poly (dA-dT) and the octamer of histones.
In this paper we describe a detailed investigation of the reconstitution of nucleosome cores from poly (dA-dT) and the octamer of histones. We also attempted the reconstitution from the copolymers poly dA.poly dT, poly dG.poly dC and poly (dG-dC). The repeat of the reconstituted chromatin fibre is discussed. The micrococcal nuclease released poly (dA-dT) core particle is found to contain a considerably narrower DNA size distribution that of the native random DNA nucleosome core (12). In addition we have succeeded in obtaining small crystals of the poly (dA-dT) nucleosome core. The DNAase I digestion pattern of the poly (dA-dT) containing nucleosome core is presented. The periodicity of DNAase I cutting sites is found to be about 10.5 bases and is similar to that of the native nucleosome core (12, 13).
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D004912:Erythrocytes / Q000032:analysis; D006657:Histones / Q000097:blood; D046911:Macromolecular Substances; D008970:Molecular Weight; D011067:Poly dA-dT; D011089:Polydeoxyribonucleotides; D011485:Protein Binding; D013329:Structure-Activity Relationship
D002843:Chromatin; D006657:Histones; D046911:Macromolecular Substances; D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT