title: Organ culture of adult mouse intestine. II. Mitotic activity, DNA synthesis and cellular migration after 24 and 48 hours of culture.
abstract: DNA synthesis, mitotic activity and cell migration have been measured in organ culture of adult mouse jejunum during the first 48 hr. Explants cultured in DMEM-HEPES-NCTC-135 medium present a sharp decrease of mitotic activity in the first hours of culture, but mitoses are restored to 80% of the controls between 24 and 48 hr. DNA synthesis follows the same pattern. Cell migration continues during culture.
mesh_terms: D000818:Animals; D002465:Cell Movement; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004386:Duodenum / Q000166:cytology* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism; D007583:Jejunum / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008938:Mitosis; D009924:Organ Culture Techniques
chemical_list: D003470:Culture Media; D004247:DNA