title: Factors affecting recombinant frequency in protoplast fusions of Streptomyces coelicolor.
abstract: The optimum concentration of polyethylene glycol 1000 (PEG) for the production of recombinants through protoplast fusion in Streptomyces coelicolor was about 50% (w/v). The addition of 14% (v/v) dimethyl sulphoxide to the fusion mixture enhanced recombination frequencies, but only at sub-optimal PEG concentrations. After treatment of protoplasts with 50% PEG for 1 min, the frequency of recombinants in a multi-factor 'cross' sometimes exceeded 20% of the total progeny. The frequency of recombinants in the progeny could be significantly enhanced by ultraviolet irradiation of the parental protoplast suspensions immediately before fusion.
mesh_terms: D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D011092:Polyethylene Glycols / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D011995:Recombination, Genetic / Q000187:drug effects / Q000528:radiation effects; D013302:Streptomyces / Q000166:cytology / Q000235:genetics*; D014466:Ultraviolet Rays
chemical_list: D011092:Polyethylene Glycols; D004121:Dimethyl Sulfoxide