A rapid method for the isolation of kidney brush border membranes.
A simple rapid method for the preparation of purified brush border membranes from rabbit kidney proximal tubules is described. The method is based on hypotonic lysis, Ca2+ aggregation of contaminants and differential centrifugation. In contrast to most other published methods, the brush border membranes are free of contamination by basolateral membranes.
D000409:Alanine / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000648:ultrastructure*; D004229:Dithiothreitol / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D007672:Kidney Cortex / Q000648:ultrastructure*; D007687:Kidney Tubules, Proximal / Q000378:metabolism / Q000648:ultrastructure*; D007700:Kinetics; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure*; D011817:Rabbits
D005947:Glucose; D000409:Alanine; D004229:Dithiothreitol