[Effect of a DNA-rubomycin complex on the molecular mass, radiosensitivity and reparation of gamma-induced single-stranded breaks in the DNA of sarcoma 180 cells].
The use of the DNA-rubomycin complex resulted in appearance of one-thread breaks (OB) in DNA and markedly increased the number of the DNA OB determined immediately after irradiation. The DNA-rubomycin complex decreased the volume of the repaired OB and induced postreparation degradation of the one-bond DNA (oDNA) in the sarcoma 180 cells. The effect of the decrease in the molecular mass of the oDNA 6 hours after the irradiation to the level of the initial damage is probably in favour of the fact that degradation takes place in the areas of the repaired breaks. The data indicated that the viscosimetric method for the analysis of the OB in DNA may be promising in estimation of the efficacy of radiation and chemotherapy of solid tumors.
D000818:Animals; D004247:DNA / Q000627:therapeutic use*; D004260:DNA Repair / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D003630:Daunorubicin / Q000627:therapeutic use*; D004307:Dose-Response Relationship, Radiation; D004338:Drug Combinations; D004353:Drug Evaluation, Preclinical; D005720:Gamma Rays / Q000627:therapeutic use; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D011836:Radiation Tolerance; D012510:Sarcoma 180 / Q000628:therapy*; D013997:Time Factors
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D004338:Drug Combinations; D004247:DNA; D003630:Daunorubicin
Vliianie kompleksa DNK-rubomitsin na molekuliarnuiu massu, radiochuvstvitel'nost'i reparatsiiu gamma-indutsirovannykh odnonitevykh razryvov v DNK kletok sarkomy 180.