Stimulation of thyroglobulin synthesis in thyroid polysomes by soluble factors from reticulocyte lysate.
The addition of rabbit reticulocyte lysate supernate to membrane-bound calf thyroid polysomes resulted in stimulation of [3H]leucine incorporation into acid-precipitable material; 91% of these total radioactive peptides was precipitated by thyroglobulin antibodies. The ability of reticulocyte lysate supernate to stimulate thyroglobulin syntheis was pH and temperature sensitive. The addition of 25 microM aurintricarboxylic acid resulted in an 85% inhibition of [3H]leucine incorporation.
D000818:Animals; D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D002417:Cattle; D007700:Kinetics; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D011817:Rabbits; D012156:Reticulocytes / Q000502:physiology*; D012270:Ribosomes / Q000378:metabolism; D013954:Thyroglobulin / Q000096:biosynthesis*; D013961:Thyroid Gland / Q000378:metabolism*
D001312:Aurintricarboxylic Acid; D013954:Thyroglobulin