Isoproterenol excretion and metabolism in the isolated perfused rat kidney.
[3H]isoproterenol excretion and metabolism were studied in the isolated perfused rat kidney using a one-pass, non-recirculating perfusion system with constant infusion rates of [3H]isoproterenol. The [3H]isoproterenol (U/P) to inulin (U/P) ratio was approximately 15 indicating extensive tubular secretion. A major renal metabolite, 3-O-methylisoproterenol, appeared in the urine and renal vein perfusate and also accumulated in the renal tissue. The fractional excretion of isoproterenol decreased with time while fractional excretion of p-aminohippurate remained stable. The observed decreasing urinary clearance and percent extraction of isoproterenol with time may be due to the progressive intrarenal accumulation of 3-O-methylisoproterenol.
D000818:Animals; D001711:Biotransformation; D007261:Infusions, Intra-Arterial; D007444:Inulin / Q000378:metabolism; D007545:Isoproterenol / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology / Q000652:urine; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008745:Methylation; D010477:Perfusion; D051381:Rats; D012077:Renal Artery; D012082:Renal Veins; D013997:Time Factors; D010130:p-Aminohippuric Acid / Q000378:metabolism
D007444:Inulin; D007545:Isoproterenol; D010130:p-Aminohippuric Acid