A platelet defect in a patient with eosinophilic leukaemia: absent ristocetin-induced platelet aggregation associated with a reduced platelet sialic acid content.
Platelets from a patient with eosinophilic leukaemia were not aggregated by ristocetin. The defect was not corrected by normal human plasma and was due to a platelet abnormality. The patient's platelets also showed a diminished sensitivity to aggregation by bovine factor VIIIVWF. The defect was not associated with a prolonged bleeding time. No abnormalities were detected in ADP, collagen or thrombin-induced platelet aggregation. Biochemical studies showed that the platelets were deficient in sialic acid. This deficiency was associated with a reduced staining for glycoprotein I following SDS-polyacrylamide gel electrophoresis. The results suggest an acquired platelet surface abnormality.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001791:Blood Platelet Disorders / Q000097:blood*; D001792:Blood Platelets / Q000378:metabolism*; D001798:Blood Proteins / Q000032:analysis; D003094:Collagen / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004804:Eosinophils; D005169:Factor VIII / Q000494:pharmacology; D006023:Glycoproteins / Q000097:blood; D006487:Hemostasis; D006801:Humans; D007938:Leukemia / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects*; D012310:Ristocetin / Q000494:pharmacology*; D012794:Sialic Acids / Q000097:blood*; D013917:Thrombin / Q000494:pharmacology
D001798:Blood Proteins; D006023:Glycoproteins; D012794:Sialic Acids; D012310:Ristocetin; D000244:Adenosine Diphosphate; D005169:Factor VIII; D003094:Collagen; D013917:Thrombin