Analysis of the serum IgE levels in nonimmunized rats of various strains by a radioimmunoassay.
An assay, the paper radioimmunosorbent test (PRIST) for the measurement of total serum IgE in the rat, that allows the measurement of IgE down to a level of 0.25 ng/ml, is described. With this assay it has been demonstrated that significant differences exist in normal serum IgE levels in non-immunized rats. These differences are strain-dependent, and strains may be classified as to low (less than 50 ng/ml), medium (50--300 ng/ml) or high (greater than 300 ng/ml) serum levels. The serum IgE level is not sex-dependent. Environmental factors have a pronounced influence, optimal housing conditions resulting in low and stable levels while suboptimal conditions result in high levels with great fluctuations. Hybridization experiments suggest a multigenic control, with low IgE strains having a dominating suppressor effect.
D000367:Age Factors; D000818:Animals; D005260:Female; D006824:Hybridization, Genetic; D007106:Immune Sera; D007073:Immunoglobulin E / Q000032:analysis*; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D008297:Male; D011863:Radioimmunoassay / Q000379:methods*; D011864:Radioimmunosorbent Test / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors
D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D007073:Immunoglobulin E