A simpler method for combined detection of human peripheral lymphocyte subpopulations.
A method for the simultaneous enumeration of human lymphocyte subpopulations was developed by mixing lymphocytes, sheep red blood cells and immunobeads. Thymus-dependent lymphocytes (T cells) are detected by rosette formation with AET-treated sheep blood cells, and thymus-independent lymphocytes (B cells) are identified by rosette formation with polyacrylamide gel coated with antibody specific to both light chains (chi and lambda) of immunoglobulin. The major advantage of this technique is that it is simple, convenient and reproducible. This method also detects lymphocytes possessing both T and B cell markers (double-marker lymphocytes). Three different types of rosettes are distinquishable under light-microscopic observation, and this method may be valuable in clinical use.
D000818:Animals; D000918:Antibody Specificity; D002469:Cell Separation; D002965:Classification; D005782:Gels / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes; D008722:Methods; D012397:Rosette Formation; D012756:Sheep; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology
D005782:Gels; D000338:beta-Aminoethyl Isothiourea