title: The binding and catalytic activities of forms of ligandin after modification of its thiol groups.
abstract: Ligandin (glutathione S-transferase B, EC 2.5.1.18)was treated with p-mercuribenzoate, N-(4-dimethylamino-3,5-dinitrophenyl)-maleimide, 5,5,-dithiobis-(2-nitrobenzoic acid), N-ethylmaleimide, iodoacetamide or iodoacetate. Although performic acid oxidation revealed the presence of four cysteines, p-mercuribenzoate and N-(4-dimethylamino-3,5-dinitrophenyl)maleimide, the most effective of the reagents studied, reacted with only three residues. N-Ethylmaleimide and 5,5'-dithiobis-(2-nitrobenzoic acid) each reacted with two cysteines: iodoacetamide reacted with only one cysteine and iodoacetate was essentially unreactive. Modification of three thiol groups decreased both the enzymic and binding activities of ligandin although the number of binding sites was unaffected. Modification of only one or two of the thiol groups had little effect on the ligandin activities. It therefore appears that there is a thiol group in the common hydrophobic-ligand- and substrate-binding site of ligandin. Ligandin was separated into two fractions on CM-cellulose. Both fractions gave the same results with p-mercuribenzoate and iodoacetamide.
mesh_terms: D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D005982:Glutathione Transferase / Q000302:isolation & purification / Q000378:metabolism*; D007461:Iodoacetates / Q000494:pharmacology; D008301:Maleimides / Q000494:pharmacology; D008626:Mercuribenzoates / Q000494:pharmacology; D011485:Protein Binding
chemical_list: D007461:Iodoacetates; D008301:Maleimides; D008626:Mercuribenzoates; C016399:N-(4-dimethylamino-3,5-dinitrophenyl)maleimide; D004228:Dithionitrobenzoic Acid; D005982:Glutathione Transferase