Reversal of 2-deoxyglucose inhibition of serotonin uptake in isolated guinea pig lung.
The effect of various substrates on the rate of uptake of 5-hydroxytryptamine (serotonin) from the pulmonary circulation was studied with isolated guinea pig lungs. Lungs were ventilated and were perfused with an electrolyte solution in a recirculating system. Uptake of serotonin was calculated from the rate of disappearance of [14C]serotonin from the perfusate. Serotonin uptake was inhibited by approximately 40% when 5 mM 2-deoxyglucose was substituted for glucose in the pulmonary perfusate. The inhibition due to 2-deoxyglucose was reversed by addition to the perfusate of pyruvate, lactate, acetate, alanine, beta-hydroxybutyrate, palmitate, or glycerol. Fructose ans succinate were less efficient substrates while no significant effect was noted with L-glycerol 1-phosphate or L-phenylalanine. These results indicate that a wide variety of metabolizable substrates can be utilized by the pulmonary endothelium to support uptake of serotonin.
D000085:Acetates / Q000494:pharmacology; D000409:Alanine / Q000494:pharmacology; D000818:Animals; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003864:Depression, Chemical; D005990:Glycerol / Q000494:pharmacology; D006168:Guinea Pigs; D006885:Hydroxybutyrates / Q000494:pharmacology; D066298:In Vitro Techniques; D007773:Lactates / Q000494:pharmacology; D008168:Lung / Q000378:metabolism*; D008297:Male; D010168:Palmitates / Q000494:pharmacology; D010477:Perfusion; D011773:Pyruvates / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism*
D000085:Acetates; D003837:Deoxy Sugars; D006885:Hydroxybutyrates; D007773:Lactates; D010168:Palmitates; D011773:Pyruvates; D012701:Serotonin; D003847:Deoxyglucose; D000409:Alanine; D005990:Glycerol