title: Ultrastructural features of acidic complex carbohydrates in the intercellular matrix of the ovarian follicles in adult mice.
abstract: In the intercellular matrix of the granulosa layer of the mouse ovarian follicles, ultrastructural features of acidic complex carbohydrates have been studied by means of dialyzed iron (DI) staining in combination with procedures of digestion with Streptomyces and testicular hyaluronidases. In the intercellular matrix, DI reactive structures containing acidic complex carbohydrates consist of layers of a variable thickness coating the plasma membrane of the granulosa cells and reticular elements distributed in the spaces between the cells. The latter exists in two appearances; one is clumped masses of irregular shapes and different sizes, whereas the other being filamentous figures radiating from the masses. The effects of digestion with Streptomyces and testicular hyaluronidases upon the DI staining of the tissues indicate that the DI reactive structures in the intercellular matrix contain at least three types of acidic complex carbohydrates; hyaluronic acid, isomeric chondroitin sulfates and other acidic glycosaminoglycans. The histophysiological activities played by these particular complex carbohydrates have been briefly discussed.
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D005110:Extracellular Space / Q000032:analysis*; D005260:Female; D006107:Granulosa Cells / Q000648:ultrastructure*; D006651:Histocytochemistry / Q000379:methods; D051379:Mice; D006080:Ovarian Follicle / Q000648:ultrastructure*
chemical_list: D002241:Carbohydrates