Structural characterization of a glycoprotein isolated from alveoli of patients with alveolar proteinosis.
A soluble glycoprotein of Mr = 80,000 has been isolated from lung lavage of patients with alveolar proteinosis and found to contain 5 residues of hydroxyproline, 91 residues of glycine, 3 residues of methionine, 3.8 molecules of sialic acid, 6 molecules of mannose, 5.9 molecules of galactose, 1 molecule of fucose, and 9.1 molecules of glucosamine. Cyanogen bromide (CNBr) treatment of the glycoprotein resulted in four peptides with molecular weights of 36,000, 27,000, 12,000, and 5,000. The chemical compositions of the CNBr peptides indicated the presence of hydroxyproline and high amounts of glycine in all but one of the peptides; two of the four CNBr peptides contained carbohydrate. Limited trypsin digestion of the glycoprotein of Mr = 80,000 resulted in four peptides with molecular weights of 62,000, 36,000, 26,000 and 18,000, the latter being the NH2-terminal peptide of the native glycoprotein molecule. The peptide of Mr = 26,000 was found to be the COOH-terminal peptide.
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D003488:Cyanogen Bromide; D006023:Glycoproteins / Q000302:isolation & purification; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D011649:Pulmonary Alveolar Proteinosis / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000032:analysis*; D014357:Trypsin
D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D014357:Trypsin; D003488:Cyanogen Bromide