Purification of leukocytosis-promoting substance from bovine parotid gland extract.
A leukocytosis-promoting substance was purified from a crude bovine parotid gland extract. The purified substance was proved to be a single component by polyacrylamide gel disc electrophoresis. It stimulates an increase of peripheral leukocyte numbers in rabbits. The molecular weight of the physiologically active component was estimated to be 4.5 . 10(4), and the component was found by sodium dodecyl sulfate polyacrylamide gel electrophoresis to be dissociated into two subcomponents.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D007964:Leukocytosis / Q000139:chemically induced*; D008297:Male; D008722:Methods; D008970:Molecular Weight; D010306:Parotid Gland / Q000032:analysis*; D011817:Rabbits; D014020:Tissue Extracts / Q000032:analysis
D014020:Tissue Extracts