Effects of restriction of amino acid supply to the isolated perfused guinea-pig mammary gland.
When individual essential amino acids were omitted for periods of 40--100 min from the infusate substrate solution in isolated perfused guinea-pig mammary gland experiments, uptake of methionine, tyrosine, phenylalanine, histidine and tryptophan (group 1) was significantly depressed by a mean of 49.8%, whereas the remaining essential amino acids (group 2) showed no significant decrease in uptake. During depletion periods oxidation of [14C]amino acids was increased. The possible significance of the differences in absorption between the 2 groups of amino acids is discussed.
D000409:Alanine / Q000378:metabolism; D000601:Amino Acids, Essential / Q000378:metabolism*; D000818:Animals; D005260:Female; D006168:Guinea Pigs / Q000378:metabolism*; D066298:In Vitro Techniques; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008894:Milk Proteins / Q000096:biosynthesis; D010477:Perfusion; D014633:Valine / Q000378:metabolism
D000601:Amino Acids, Essential; D008894:Milk Proteins; D014633:Valine; D000409:Alanine