Competitive binding radioassay for 5-fluorodeoxyuridine 5'-monophosphate in tissues.
A competitive binding radioassay has been developed for 5-fluorodeoxyuridine 5'-monophosphate, based on the tight binding of this potent inhibitor to thymidylate synthetase (EC 2.1.1.45). Unbound ligand may be separated from that bound to enzyme by precipitating the intact inhibitor-enzyme complex with trichloroacetic acid. Scatchard plot analysis using a two-site model for binding yielded apparent dissociation constants of 1.2 x 10(-11) and 1.7 x 10(-10) M from a least-squares computer fit of the data. 5-Fluorodeoxyuridine 5'-monophosphate could be detected in the range of 0.02 to 2.0 pmol with no apparent interference by other substances. Assay of 5-fluorodeoxyuridine 5'-monophosphate levels in L1210 ascites tumor following 5-fluorouracil in vivo revealed peak levels occurring within the first hr with a subsequent disappearance half-life of 3.9 hr. Close agreement was found between the previously described enzyme inhibition assay and the more rapid and sensitive competitive binding method.
D000818:Animals; D001667:Binding, Competitive; D003856:Deoxyuracil Nucleotides / Q000032:analysis*; D005468:Fluorodeoxyuridylate / Q000032:analysis*; D005472:Fluorouracil / Q000627:therapeutic use; D006207:Half-Life; D007939:Leukemia L1210 / Q000032:analysis; D051379:Mice; D011869:Radioligand Assay / Q000379:methods*; D013940:Thymidylate Synthase
D003856:Deoxyuracil Nucleotides; D005468:Fluorodeoxyuridylate; D013940:Thymidylate Synthase; D005472:Fluorouracil