Glycerophospho-(N-acyl)-ethanolamine lipids in degenerating BHK cells.
The phospholipids, which accompany semilysobisphosphatidic acid from degenerating BHK cells, were identified as a mixture of glycerophospho-(N-acyl)-ethanolamine lipids. The identification was based on infrared spectroscopy, thin-layer chromatography and ethanolamine content of the intact lipids or their partial degradation products. Sequential treatments with mild acid and alkali revealed the presence of three different derivatives: the most abundant of these was the O-(1-alkenyl) ether derivative (plasmenyl-(N-acyl)-ethanolamine), which represented 55-60% of the total glycerophospho-(N-acyl)-ethanolamine lipids; the O-alkyl derivative (plasmanyl-(N-acyl)-ethanolamine) and the di-O-acyl derivative (phosphatidyl-(N-acyl)-ethanolamine) each represented about 20% of the total.
D002854:Chromatography, Paper; D002999:Clone Cells; D010714:Phosphatidylethanolamines / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D013055:Spectrophotometry, Infrared
D010714:Phosphatidylethanolamines; D010743:Phospholipids