Uptake and metabolism of [3H]norepinephrine in uterine nerves of pregnant guinea pig.
Myometrial tissue slices from virgin, pregnant (uni- or bilateral pregnancy), and puerperal animals were incubated in media containing [3H]norepinephrine ([3H]NE), and the neuronal and extraneuronal uptake was estimated. The metabolic fate of [3H]NE was elucidated by the chromatographic separation of [3H]NE, [3H]normetanephrine and 3H-labeled acid metabolites. An early and extensive reduction in both total and neuronal uptakes occurred in the myometrial regions surrounding the fetuses, and at term pregnancy no neuronal [3H]NE uptake at all was found in tissue slices from the fetus-containing horns. Concomitantly, the fraction of unchanged [3H]NE decreased with a corresponding increment in its metabolites. Similar changes, though less extensive, also occurred in the empty horn in unilateral pregnancy. The total amount of neuronal uptake in the cervix seemed unchanged compared to virgin animals. Earlier and present data support the following suggestion that in uterine horns harboring fetuses, an extensive axonal degeneration occurs; in those devoid of fetuses (unilateral pregnancy), the nerve terminals are to a great extent structurally intact, but functionally damaged. Recovery of the pregnancy-induced impairment of the adrenergic nerve plexus is slow and incomplete.
D000320:Adrenergic Fibers / Q000378:metabolism*; D000818:Animals; D002584:Cervix Uteri / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D009215:Myometrium / Q000294:innervation* / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal; D013997:Time Factors; D014316:Tritium; D014599:Uterus / Q000294:innervation*
D014316:Tritium; D009638:Norepinephrine