title: An improved extraction method for plasma steroid hormones.
abstract: An easy and rapid column chromatographic method for the extraction of steroid hormones from plasma is presented. It permits the nearly quantitative separation of the steroids in a single step with smaller expenditure of time and work as compared to the usual liquid-liquid extraction. Problems of emulsions are eliminated. Furthermore, fractionated separation of hormones from plasma is possible. A simple procedure for the selective extraction of estriol (isolated from estrone and estradiol) is described.
mesh_terms: D002845:Chromatography / Q000379:methods; D003345:Corticosterone / Q000097:blood*; D004958:Estradiol / Q000097:blood; D004964:Estriol / Q000097:blood; D004967:Estrogens / Q000097:blood*; D004970:Estrone / Q000097:blood; D006801:Humans; D011374:Progesterone / Q000097:blood*
chemical_list: D004967:Estrogens; D004970:Estrone; D011374:Progesterone; D004958:Estradiol; D004964:Estriol; D003345:Corticosterone