title: Isatin-enzyme interactions. VII. Mechanism of inhibition of rat kidney alkaline phosphatase.
abstract: Isatin has been found to inhibit rat kidney alkaline phosphatase (EC 3.1.3.1). The inhibition is dependent on isatin concentration and is of un-competitive type. The hydrolysis of disodium phenyl phosphate by the enzyme at different temperatures (17--37 degrees C) obeys the Arrhenius equation. Energy of activation in the absence and presence of isatin has been found to be 9.84 and 10.24 kCal/mol. The hyperbolic profile of isatin inhibition; the lowering of both Km and Vmax in the presence of isatin, and, small changes in enthalpy, free energy and entropy in the presence of isatin suggest a non-allosteric un-competitive inhibition of the enzyme.
mesh_terms: D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors; D000818:Animals; D007211:Indoles / Q000494:pharmacology*; D007510:Isatin / Q000494:pharmacology*; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D051381:Rats; D013696:Temperature; D013816:Thermodynamics
chemical_list: D007211:Indoles; D007510:Isatin; D000469:Alkaline Phosphatase