Free-flow electrophoresis for the separation of malaria infected and uninfected mouse erythrocytes and for the isolation of free parasites (Plasmodium vinckei): a new rapid technique for the liberation of malaria parasites from their host cells.
After aggregation of erythrocytes from malaria infected mice, the parasites (Plasmodium vinckei) could be set free using gentle mechanical forces. The mixture of freed parasites, infected and non-infected erythrocytes, and membraneous material was separated by free-flow electrophoresis. The free parasites produced were very pure and infectious. Morphological and enzymatic data on the separated fractions are presented. Free-flow electrophoresis also allowed the separation of infected and uninfected erythrocytes.
D000818:Animals; D002458:Cell Fractionation; D004586:Electrophoresis / Q000379:methods; D004903:Erythrocyte Aggregation; D004912:Erythrocytes / Q000469:parasitology*; D005260:Female; D008288:Malaria / Q000469:parasitology*; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D010961:Plasmodium / Q000302:isolation & purification*