Purification and characterization of 3,4-dihydroxyphenylalanine decarboxyase from pig kidney.
A procedure for 3,4-dihydroxyphenylalanine decarboxylase from pig kkdney purification is described in detail. The preparation has no detectable impurity on electrophoresis and on ultracentrifugation and authors. However two significant differences are observed: a different stimulation of activity by added pyridoxal 5'-phosphate and a nearly complete decarboxylation of L-3,4-dihydroxyphenylalanine in absence of added coenzyme. Absorption, fluorescence and circular dichroism properties of the coenzyme-apoenzyme interaction are also described. The results are consistent with the existence of at least four coenzyme-apoenzyme complexes, three of them active.
D000818:Animals; D001051:Apoenzymes; D002942:Circular Dichroism; D004296:Dopa Decarboxylase / Q000302:isolation & purification*; D007668:Kidney / Q000201:enzymology*; D011487:Protein Conformation; D011732:Pyridoxal Phosphate; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013552:Swine
D001051:Apoenzymes; D011732:Pyridoxal Phosphate; D004296:Dopa Decarboxylase