Characterization of measles virus-specific proteins synthesized in vivo and in vitro from acutely and persistently infected cells.
Measles virus protein synthesis has been analyzed in acutely and persistently infected cells. To assess the role of measles in subacute sclerosing panencephalitis (SSPE), measles viral proteins synthesized in vivo or in vitro were tested for reactivity with serum from a guinea pig(s) immunized with measles virus and sera from patients with SSPE. Guinea pig antimeasles virus serum immunoprecipitates the viral polypeptides of 78,000 molecular weight (glycosylated [G]), 70,000 molecular weight (phosphorylated [P]), 60,000 molecular weight (nucleocapsid [N]), and 35,000 molecular weight (matrix [M]) from cells acutely infected with measles virus as well as from chronically infected cells, but in the latter case, immunoprecipitated M protein has a reduced electrophoretic migration. Sera of SSPE patients immunoprecipitated all but the G protein in acutely infected cells and only the P and N proteins from chronically infected cells. In immunoprecipitates of viral polypeptides synthesized in a reticulocyte cell-free translation system, in response to mRNA from acutely or persistently infected cells, the 78,000-molecular-weight form of the G protein was not detected among the cell-free products of either mRNA. Guinea pig antimeasles virus serum immunoprecipitated P, N, and M polypeptides from the products of either form of mRNA, whereas SSPE serum immunoprecipitated the P and N polypeptides but not the M polypeptide. The differences in immunoreactivity of the antimeasles virus antiserum and the SSPE serum are discussed in terms of possible modifications of measles virus proteins in SSPE.
D002460:Cell Line; D002474:Cell-Free System; D006801:Humans; D007106:Immune Sera; D008459:Measles virus / Q000276:immunology / Q000378:metabolism*; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010455:Peptides / Q000276:immunology; D013344:Subacute Sclerosing Panencephalitis / Q000276:immunology; D014764:Viral Proteins / Q000096:biosynthesis* / Q000276:immunology
D007106:Immune Sera; D010455:Peptides; D014764:Viral Proteins