Localization of acid and alkaline phosphatase activities in the blood vessels of normotensive and hypertensive rats.
Samples of aortae and caudal arteries from normotensive and hypertensive rats were studied for cytochemical and biochemical determinations of acid and alkaline phosphatase activities. Cytochemical examination revealed an increased amount of acid phosphatase reaction product in hypertensive samples, with extensive localization to the extracellular matrix. Alkaline phosphatase activity was localized to the plasma membrane of fibroblasts and vascular smooth muscle cells and to the extracellular matrix. Biochemical assays of enzyme activities supported the cytochemical findings, showing increased activity in aortae from hypertensive rats.
D000135:Acid Phosphatase / Q000097:blood*; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D001013:Aorta, Thoracic / Q000201:enzymology*; D001794:Blood Pressure; D003900:Desoxycorticosterone / Q000494:pharmacology; D006973:Hypertension / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D009130:Muscle, Smooth / Q000648:ultrastructure; D051381:Rats; D013623:Tail / Q000098:blood supply*
D003900:Desoxycorticosterone; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase