Re-evaluation of the uptake of desoxyfructo-serotonin by human platelets, and of its effect on the uptake of serotonin.
Employing C14-glucose and C14-serotonin (5HT) as precursors, we have synthesized desoxyfructo-5HT [1-desoxy-1-(5-hydroxytryptamine)-D-fructose] labelled either in the sugar or 5HT moieties, and examined the uptake of the labelled substrate by washed human platelets. Desoxyfructo-5HT appears to interact with the 5HT uptake site, effectively inhibiting the uptake of H3-5HT. However, our data indicate that contrary to published reports, the entry of desoxyfructo-5HT into platelet vesicles or cytoplasm is negligible.
D001711:Biotransformation; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D005632:Fructose / Q000031:analogs & derivatives / Q000097:blood / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D012701:Serotonin / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D005632:Fructose; D012701:Serotonin