Heparin induced alterations in clearance and distribution of blood-borne microparticles following operative trauma.
The influence of systemic heparin administration on the vascular clearance and tissue distribution of blood-borne microparticles was evaluated in normal rats and rats after operation (laparotomy plus intestinal manipulation) utilizing an (131)I- colloid which is phagocytized by the reticuloendothelial system (RES). Intravenous heparin administration (100 USP/100g body weight) into normal animals three minutes prior to colloid injection (50 mg/lOOg) induced a significant increase in pulmonary localization of the microparticles as compared to nonheparinized control rats, while hepatic and splenic uptake were decreased. Surgical trauma decreased hepatic RE uptake and increased pulmonary localization of the microparticles when injected systemically at 60 minutes postsurgery. Heparin administration 60 minutes after surgery and three minutes prior to colloid injection, magnified the increased pulmonary localization response with an associated further depression of the RES. The ability of heparin to alter both RE clearance function and lung localization of microparticles was dose dependent and a function of the interval between heparin administration and systemic particulate infusion. Thus, low dose heparin administration was capable of stimulating RE activity while heparin in doses of excess of 50 USP units/lOOg body weight decreased RE function. These findings suggest that the functional state of the hepatic RE system can be greatly affected in a dose-dependent manner by systemic heparin administration which may influence distribution of blood-borne microparticles.
D000818:Animals; D003102:Colloids / Q000097:blood*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005990:Glycerol / Q000097:blood; D006493:Heparin / Q000008:administration & dosage / Q000494:pharmacology*; D007275:Injections, Intravenous; D007457:Iodine Radioisotopes; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010587:Phagocytosis / Q000187:drug effects*; D010713:Phosphatidylcholines / Q000097:blood; D051381:Rats; D013514:Surgical Procedures, Operative / Q000009:adverse effects*
D003102:Colloids; D007457:Iodine Radioisotopes; D010713:Phosphatidylcholines; D006493:Heparin; D005990:Glycerol