title: [Preparation and characteristics of temperature-sensitive mutants of the Venezuelan equine encephalomyelitis virus].
abstract: Fifteen ts mutants of Venezuelan equine encephalomyelitis (VEE) virus have been produced and studied. Two of them were isolated from a virus population grown in the presence of 5-fluorouracyl and 13 from a virus suspension treated with nitrous acid. The efficiency of plating 40 degrees/35 degrees of various mutants varied from 2 X 10(-2)  to 6 X 10(-6). Three ts mutants had RNA- phenotype (RNA synthesis at a nonpermissive temperature was 1.5-3.3% of that of the wild type), 11 ts mutants had RNA+ phenotype (RNA synthesis decreased insignificantly, 32-75%), and one ts mutant was intermediate (18%).
mesh_terms: D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005472:Fluorouracil / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects; D009608:Nitrous Acid / Q000494:pharmacology; D010641:Phenotype; D012367:RNA, Viral / Q000096:biosynthesis; D013696:Temperature; D010948:Viral Plaque Assay
chemical_list: D012367:RNA, Viral; D009608:Nitrous Acid; D005472:Fluorouracil
vernacular_title: Poluchenie i kharakteristika temperaturochuvsvtitel'nykh mutantov virusa venesuél'skogo éntsefalomielita loshadeĭ.