Failure to confirm specificity of samples containing a very high titer of hepatitis B surface antigen (HBsAg).
Two samples repeatedly positive for HBsAg by the AusRIA II screening procedure failed to be confirmed by neutralization with anti-HBs serum. Both samples could be effectively neutralized by the same anti-HBs sera if diluted 1:10, 1:50 and 1:100 prior to testing. It is suggested that the inability to neutralize was due to a very high concentration of HBsAg in these samples.
D000328:Adult; D001782:Blood Donors; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D006386:Hemagglutination Tests; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D011863:Radioimmunoassay
D006514:Hepatitis B Surface Antigens; D004259:DNA-Directed DNA Polymerase