title: Effect of CCK-octapeptide and secretin on amylase secretion in isolated rat pancreatic acinar cells.
abstract: Isolated acinar cells from rat pancreas responded well to hormonal treatment. Both secretin (synthetic and highly purified from porcine origin) and CCK-octapeptide stimulated amylase secretion in these cells. The response in both cases was very rapid. A maximal output of enzyme was reached within 5-10 min after the addition of hormones. The concentration producing maximal output for synthetic secretin (Schwarz/Mann) was 5 x 10(-8) M, synthetic secretin (Squibb), 10(-5) M, for purified porcine secretin, 10(-5) M, and for CCK-octapeptide was 5 x 10(-10) M. Secretin (2-fold at optimal concentration) was found to be less efficient compared to CCK-octapeptide (5-fold at optimal concentration) in stimulating amylase release. A combination of secretin and CCK-octapeptide had a synergistic action in stimulating enzyme release by the acinar cells. In addition, pretreatment of acinar cells with secretin potentiated the secretory response of the treated cells to CCK-octapeptide. To a lesser extent pretreatment with CCK-octapeptide also increased the effect of secretin in stimulating enzyme secretion.
mesh_terms: D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002469:Cell Separation; D002766:Cholecystokinin / Q000494:pharmacology*; D008297:Male; D010179:Pancreas / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D012633:Secretin / Q000494:pharmacology*; D012636:Secretory Rate / Q000187:drug effects
chemical_list: D012633:Secretin; D002766:Cholecystokinin; D000681:Amylases