Photodynamic inactivation of cancer cells in vitro. Effect of irradiation temperature and dose fractionation.
Human cancer cells in vitro (NHIK 3025), derived from a carcinoma in situ are inactivated when exposed to visible light in the presence of hematoporphyrin. Irradiation at 4 degrees C results in much more efficient inactivation than irradiation at 37 degrees C. Furthermore, a given total light dose is more efficient when it is fractionated than when it is given in a single exposire. These findings may be taken advantage of in future photochemotherapy of cancer.
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D006415:Hematoporphyrins / Q000494:pharmacology*; D006801:Humans; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D010778:Photochemotherapy; D013696:Temperature; D013997:Time Factors
D006415:Hematoporphyrins