A microassay for the measurement of androgen receptors in human prostatic tissue.
A microassay utilizing R 1881 (methyltrienolone) has been developed for the measurement of androgen receptor sites in the cytosol and nuclear extract of human prostatic tissue. Binding of R 1881 to the progesterone binding molecule in cytosol was eliminated by the addition of triamcinolone acetonide. Utilizing a six tube, single point assay, the number of binding sites estimated in nuclear extract averaged 95% of the number measured by a full 7 point Scatchard analysis; the number estimated by the microassay in cytosol averaged 91%. When the single point assay was applied to needle biopsy specimens (200 mg of tissue), the estimated number of binding sites in nuclei averageed 83% of the number measured in bulk tissue (2 grams) utilizing a 7 point Scatchard analysis; the number in cytosol estimated by the microassay on needle biopsy specimens averaged 73%. It is hoped that this technique may be useful in correlating receptor content with hormonal responsiveness in men with metastatic carcinoma of the prostate.
D001667:Binding, Competitive; D002467:Cell Nucleus / Q000032:analysis; D003600:Cytosol / Q000032:analysis; D004963:Estrenes / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008297:Male; D008832:Microchemistry; D011467:Prostate / Q000032:analysis*; D011944:Receptors, Androgen / Q000032:analysis*; D011987:Receptors, Steroid / Q000032:analysis*; D045165:Testosterone Congeners / Q000378:metabolism; D014222:Triamcinolone Acetonide / Q000378:metabolism
D004963:Estrenes; D011944:Receptors, Androgen; D011987:Receptors, Steroid; D045165:Testosterone Congeners; D014222:Triamcinolone Acetonide