Monoclonal immunoglobulin rescue from a patient with chronic lymphocytic leukemia and autoimmune hemolytic anemia.
In order to test whether autoimmune hemolytic anemia in a patient with chronic lymphocytic leukemia was due to autoantibody production by the neoplastic clone, somatic cell hybrids were constructed between the patient's leukemic cells and a mouse myeloma line. Light-chain-restricted human immunoglobulin was secreted by 4 of 11 of the established hybrid lines. No binding of this immunoglobulin to red cell surface antigens could be detected. Thus, we conclude that the autoantibody in this patient represented a concomitant reactive response and was not the product of the malignant clone of B cells.
D000368:Aged; D000744:Anemia, Hemolytic, Autoimmune / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis; D007945:Leukemia, Lymphoid / Q000276:immunology*; D009194:Myeloma Proteins / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D001323:Autoantibodies; D007075:Immunoglobulin M; D009194:Myeloma Proteins; D011947:Receptors, Antigen, B-Cell