Extraction of glucose isomerase from Streptomyces flavogriseus.
Cationic detergent (cetyltrimethylammonium bromide or cetylpyridinium chloride) treatment extracted almost the same amount of glucose isomerase from cells of Streptomyces flavogriseus as mechanical disruption (sonic oscillation or abrasive grinding). The specific activity of the enzyme extracted with cationic detergents was approximately 20% higher than that liberated by mechanical disruption.
D001433:Bacteriolysis; D002238:Carbohydrate Epimerases / Q000302:isolation & purification* / Q000378:metabolism; D002474:Cell-Free System; D002593:Cetrimonium Compounds / Q000494:pharmacology; D002594:Cetylpyridinium / Q000494:pharmacology; D012988:Soil Microbiology; D013010:Sonication; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology*
D002593:Cetrimonium Compounds; D002594:Cetylpyridinium; D002238:Carbohydrate Epimerases