title: [Inhibition of succinate and NADH oxidases of submitochondrial particles by iron chelators and sulfhydryl reagents].
abstract: The inhibition of succinate- and NADH-oxidase activities of submitochondrial particles by 4,7-diphenyl-1,10-phenantroline was studied. The inhibition was shown to increase when the particles were pretreated with SH-reagents. The treatment of submitochondrial particles with ethanol in the presence of 1,10-phenantroline resulted in a complete inactivation of succinate oxidase and succinate: tetramethyl-n-phenyldiamine reductase; the succinate PMS reductase activity was only partially inhibited after such treatment. It is concluded that tetramethyl-n-phenyldiamine and phenazine metasulfate react with different sites of the succinate dehydrogenase complex. The changes in the properties of submitochondrial particles after ethanol--phenantroline treatment are apparently due to the effect of non-polar solvent rather than to the extraction of non-haem iron.
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology; D007501:Iron; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D013367:Submitochondrial Particles / Q000201:enzymology*; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors*; D013439:Sulfhydryl Reagents / Q000494:pharmacology
chemical_list: D002614:Chelating Agents; D013439:Sulfhydryl Reagents; D007501:Iron; D013385:Succinate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases
vernacular_title: Tormozhenie suktsinat-i NADH-oksidaznykh aktivnosteĭ submitokhondrial'nykh chastits zhelezokompleksonami i sul'fgidril'nymi reagentami.