title: Partial regeneration of the above-elbow amputated rat forelimb. II. Electrical and mechanical facilitation.
abstract: This investigation was undertaken to examine the observations of Becker ('72) pertaining to the electrical facilitation of partial limb regenerative responses by means of Ag-Pt wire couples applied to the limb stumps of young, forelimb-amputated white rats. Additionally, in order to examine the possible role of mechanical effects of such device implantations, we have employed uncoupled devices delivering no current or potential difference. In the present experiments, in response to coupled device implantation, cartilage and bone were actively formed in the vicinity of the Pt electrode tip. These tissues contributed to the lengthwise extension of the limb and to the partial restoration of the distal humeral extremity. In limbs bearing the uncoupled electrical devices, qualitatively similar responses were noted, but osteogenesis was diminished in extent compared to that seen in limbs bearing the active or coupled devices. It is therefore necessary to consider the role of mechanical factors in the elicitation of the observed regenerative responses. Myogenesis was enhanced in electrically stimulated limbs, but not in those rats bearing uncoupled devices.
mesh_terms: D000671:Amputation, Surgical; D000818:Animals; D001861:Bone Regeneration; D002356:Cartilage / Q000033:anatomy & histology / Q000502:physiology; D004558:Electric Stimulation / Q000295:instrumentation; D004567:Electrodes, Implanted; D005552:Forelimb / Q000502:physiology* / Q000601:surgery; D006811:Humerus / Q000033:anatomy & histology / Q000502:physiology* / Q000601:surgery; D008297:Male; D009132:Muscles / Q000502:physiology; D010012:Osteogenesis; D010812:Physical Stimulation; D051381:Rats