title: Superoxide dismutase and O2 lethality in Bacteroides fragilis.
abstract: Exposure of midlog Bacteroides fragils (VPI 2393) to 2% O2-98% N2 caused a three- to fivefold increase in superoxide dismutase specific activity within the cells. The increase in specific activity was completed within 90 min after exposure to oxygen and was dependent upon protein synthesis. Cells containing the higher superoxide dismutase level were more resistant to the effects of 5 atm of oxygen tension than were cells containing the lower level of superoxide dismutase but were equally resistant to 5 atm of nitrogen tension. Similar results were observed upon comparing viability experiments with B. fragilis and B. vulgatus. Superoxide dismutase activity in sonic extracts of B. fragilis was rapidly inactivated by exposure to 5 mM H2O2 and was inhibited by 1 mM NaN3 but not 5 mM NaCN. The inhibition pattern is identical to the pattern demonstrated for the purified iron-containing enzyme from Escherichia coli B and suggests that the superoxide dismutase in B. fragilis is an iron enzyme.
mesh_terms: D001386:Azides / Q000494:pharmacology; D001439:Bacteroides / Q000201:enzymology; D001441:Bacteroides fragilis / Q000187:drug effects / Q000201:enzymology*; D004790:Enzyme Induction; D006861:Hydrogen Peroxide / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D001386:Azides; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D010100:Oxygen