Malignancy-associated DNA-binding protein C3DP from human serum. Further characterization and purification of C3DP retaining its DNA-binding affinity.
C3DP, a malignancy-associated DNA-binding protein from human serum[1], was purified to homogeneity without loss of its DNA-binding affinity. For this purpose normal human serum was submitted to affinity chromatography on Con A-Sepharose and DNA-cellulose and to preparative polyacrylamide gel electrophoresis. The purified C3DP was identified by immunodiffusion and sodium dodecylsulfate polyacrylamide gel electrophoresis and it was shown to bind to DNA by DNA-cellulose chromatography. The isoelectric point of C3DP was determined to 4.9 by isoelectric focusing.
D002846:Chromatography, Affinity; D004247:DNA; D006801:Humans; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000097:blood* / Q000302:isolation & purification; D009369:Neoplasms / Q000097:blood*; D011485:Protein Binding
D009363:Neoplasm Proteins; D004247:DNA