title: Preparation of an antibody to mouse serum IgD.
abstract: Mice have recently been shown to have serum IgD. We have used affinity chromatography to partially purify mouse serum IgD, and have prepared a rabbit antiserum against this mouse Ig class. This antiserum, once adsorbed by IgD-depleted mouse serum bound to Sepharose, was isotype specific as determined by radioimmunoassay and fluorescence activated cell sorter analysis, and bound to the same splenic lymphocyte surface molecules as a hybridoma produced monoclonal anti-mouse delta antibody.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001665:Binding Sites; D002846:Chromatography, Affinity; D007106:Immune Sera / Q000494:pharmacology; D007072:Immunoglobulin D / Q000276:immunology* / Q000302:isolation & purification; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013154:Spleen / Q000276:immunology
chemical_list: D000906:Antibodies; D007106:Immune Sera; D007072:Immunoglobulin D