title: Stability of isoenzymes of alkaline phosphatase in various buffer systems.
abstract: The stability of isoenzymes of alkaline phosphatase from liver, bones and small intestine was compared after addition to inactivated serum in the buffer systems: glycine, 2-amino-2-methyl-1-propanol, diethanolamine and 2-amino-2-methyl-1,3-propandiol at 37 degrees C. The mentioned isoenzymes were inactivated to different extents in glycine and 2-amino-2-methyl-1-propanol buffers. In diethanolamine and 2-amino-2-methyl-1,3-propandiol buffers sufficient stability of isoenzymes is obtained so that only these buffers are suitable for activity determinations of alkaline phosphatase at 37 degrees C.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D001842:Bone and Bones / Q000201:enzymology; D002021:Buffers; D004355:Drug Stability; D004983:Ethanolamines / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology; D006801:Humans; D007421:Intestine, Small / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D011412:Propanolamines / Q000494:pharmacology; D011409:Propylene Glycols / Q000494:pharmacology
chemical_list: D002021:Buffers; D004983:Ethanolamines; D007527:Isoenzymes; D011412:Propanolamines; D011409:Propylene Glycols; C019250:2-amino-2-methyl-1,3-propandiol; D000469:Alkaline Phosphatase; D005998:Glycine