Latent lymphokines: isolation of guinea pig latent lymphocyte-derived chemotactic factor for monocytes. Its activation by trypsin and a soluble factor from macrophages.
Guinea pig lymphocytes when depleted of macrophages and stimulated by the T cell mitogen phytohemagglutinin produce a latent form of lymphocyte-derived chemotactic factor for monocytes (LDCF-M). Latent LDCF-M is also produced when immune lymphocytes are stimulated in vitro with specific antigen, horseradish peroxidase. Latent LDCF-M from both sources can be activated and converted to "classical" LDCF-M by trypsin and by a soluble factor obtained from sonicated macrophages. These observations suggest that macrophages may modulate lymphokine activities in vivo by releasing soluble factors that convert inactive latent lymphokines to biologically active substances.
D000818:Animals; D000941:Antigens / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D002850:Chromatography, Gel; D006168:Guinea Pigs; D008214:Lymphocytes / Q000276:immunology; D008222:Lymphokines / Q000096:biosynthesis / Q000302:isolation & purification*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D008970:Molecular Weight; D009000:Monocytes / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology*
D000941:Antigens; D008222:Lymphokines; D008934:Mitogens; D010835:Phytohemagglutinins; D014357:Trypsin