Polyadenylated RNA in the lower eukaryote Physarum polycephalum.
Utilizing a method which quantitatively extracts high molecular weight RNA, including intact precursor as well as mature ribosomal RNAs, adenylated molecules have been isolated from nuclear- and cytoplasmic-enriched fractions of Physarum microplasmodia labeled with [3H]-uridine. Electrophoretic analysis of denatured adenylated RNA from the nuclear-enriched fraction indicated the presence of a population of large molecules not found in the cytoplasmic-enriched fraction.
D002467:Cell Nucleus / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D009691:Nucleic Acid Denaturation; D010804:Physarum / Q000032:analysis*; D011061:Poly A; D012332:RNA, Heterogeneous Nuclear / Q000032:analysis*; D013696:Temperature
D012332:RNA, Heterogeneous Nuclear; D011061:Poly A