Gonadotrophin-induced accumulation of 'interstitial fluid' in the rat testis.
'Interstitial fluid' containing high levels of testosterone (60-250 ng/ml) was recovered from the testes of rats, the amounts increasing with increase in age and testis weight. Injection of 170 i.u. hCG/kg resulted 20 h later in significant increases in interstitial fluid and its testosterone content (300-800 ng/ml). In immature rats this effect of hCG was dose-dependent and time-related and the accumulated fluid contained high levels of potassium and phosphate; levels of sodium, calcium and protein were similar to those in serum. At 20 h after injection of hCG, other testicular changes were (1) increased 'adhesiveness', (2) reduced in-vitro binding of 125I-labelled hCG, and (3) an hCG-induced increase in the testis:blood ratio of hCG in vivo.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000378:metabolism / Q000494:pharmacology*; D005110:Extracellular Space / Q000378:metabolism*; D008297:Male; D051381:Rats; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D013739:Testosterone