title: Cellular autofluorescence--is it due to flavins?
abstract: For many cell types, cellular autofluorescence in the 500-600 nm spectral region can result in a significant background signal for measurements of weakly fluorescent probes. Measurements of fluorescence intensity and spectra of a variety of cell types and assays of neuronal homogenates are presented to demonstrate that this autofluorescence is most likely due to endogenous flavoproteins.
mesh_terms: D000818:Animals; D002468:Cell Physiological Phenomena; D002477:Cells / Q000378:metabolism; D005420:Flavoproteins / Q000378:metabolism; D005453:Fluorescence; D009474:Neurons / Q000502:physiology; D013050:Spectrometry, Fluorescence
chemical_list: D005420:Flavoproteins