[Effect of ATP on protein synthesis in cultured nerve tissue of the edible snail].
External ATP in concentrations of 10(-6)--10(-3) M is shown to stimulate the label incorporation from intracellular labeled pool of 14C-leucine into proteins of mollusc nervous tissue. The maximum effect (by 45% higher than in control) is observed at the 10(-5) ATP concentration. In solutions with high concentration of bivalent ions, ATP action increases by 10--15%. Being incubated for an hour in physiological solutions without energic substrates nervous tissue loses 30--50% of labeled amino acids. Outwashing of 14C-leucine depends only a little on the bivalent ion concentration in the external solution and on the presence of helating agents. Addition of 10(-4) M ATP into the solution, completely inhibits the washing of amino acids out of tissue. At low bivalent ion concentrations 14C-leucine incorporation into nervous tissue in the presence of ATP changes inversely to the ATP concentration: low ATP concentrations (10(-5)--10(-6) M) activate label incorporation by 60--40%, whereas high concentrations lead to the corresponding inhibition. This inhibition is due to helating action of ATP.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D006372:Helix, Snails / Q000187:drug effects*; D007930:Leucine / Q000378:metabolism; D009417:Nerve Tissue / Q000187:drug effects*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*
D000596:Amino Acids; D009419:Nerve Tissue Proteins; D000255:Adenosine Triphosphate; D007930:Leucine
Vliianie ATF na sintez belka v kul'tiviruemoĭ nervnoĭ tkani vinogradnoĭ ulitki.