High-performance liquid chromatographic assay for imipramine, desipramine, and their 2-hydroxylated metabolites.
High-performance liquid chromatographic method is presented for the simultaneous determination of imipramine, desipramine, and their 2-hydroxylated metabolites in plasma. The method involves a simple plasma extraction at basic pH with organic solvent, chromatography on a silica gel column, and fluorescence detection. Correlation with a GLC-mass spectrometric method for imipramine and desipramine is illustrated. The method can detect 1 ng of each component/ml of plasma, sufficient sensitivity for pharmocokinetic studies.
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003891:Desipramine / Q000097:blood*; D006801:Humans; D006900:Hydroxylation; D007099:Imipramine / Q000097:blood*; D013058:Mass Spectrometry; D008722:Methods
D007099:Imipramine; D003891:Desipramine