[Method of determining tissue renin activity using heterologous serum].
The authors described a method for determination of tissue renin activity with heterologous substrate. The preparation of the substrate was performed at several stages: salting with amonium sulfate; dialisis of the precipitate till complete separation of amonium sulfate molecules; distruction of angiotensinases by interchangeble souring and alcalization of the medium; lyophylization of the pure substrate. The obtained renin-substrate was preserved in ampules and its usage had a series of advantages--duration, economic, a possibility for standartization of the determination, etc., which were described in details in the article. The described in details also the quantitative determination of the renin activity in the tissues (renal and cerebral) with the help of the obtained substrate as the moments, modiied by the authors, were indicated.
D000818:Animals; D001923:Brain Chemistry; D006736:Horses; D007106:Immune Sera / Q000302:isolation & purification; D007668:Kidney / Q000032:analysis; D007700:Kinetics; D008433:Mathematics; D008722:Methods; D051381:Rats; D012083:Renin / Q000032:analysis*
D007106:Immune Sera; D012083:Renin
Metod za opredeliane na tukanna reninova aktivnost s kheterolozhen serum.