title: [Determination of lysozyme in biological fluids by a semi-automatic kinetic technique. Discussion of the method (author's transl)].
abstract: A rapid and semi-automatic determination of lysozyme in biological fluids using kinetic analysis and turbidimetry is described and compared to other commonly used techniques. The specificity of the method is satisfactory while that of clarification of a gel medium is apparently not. Normal values and standard errors for plasma, urine, and leucocytes are given. A standardised expression of lysozyme activity is proposed and discussed. The importance of the means by which the blood sample is collected and prepared is underlined: plasma, decanted soon after collection, is preferable to serum.
mesh_terms: D001322:Autoanalysis; D001681:Biological Assay; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007962:Leukocytes / Q000201:enzymology; D008297:Male; D008837:Micrococcus; D009113:Muramidase / Q000032:analysis* / Q000097:blood / Q000652:urine; D009391:Nephelometry and Turbidimetry / Q000379:methods; D012016:Reference Values; D012737:Sex Factors
chemical_list: D009113:Muramidase
vernacular_title: Détermination du lysozyme dans les liquides biologiques par une technique cińetique semi-automatique. Discussion de la méthode.