A bichromatic method for total bilirubin with a CentrifiChem 400.
We describe a method for the bichromatic determination of total bilirubin with a CentrifiChem 400 centrifugal analyzer with the Boehringer DPD kit. After reading the blank at 620 nm, we read the test at 550 nm. Because only one rotor is required, the analytical time for bilirubin estimations is halved. The proposed method is no more prone to interference by hemoglobin or turbidity than a conventional approach or a reference continuous-flow method and has performed well over several months of routine use.
D001322:Autoanalysis; D001663:Bilirubin / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D011786:Quality Control; D011933:Reagent Kits, Diagnostic; D013053:Spectrophotometry / Q000379:methods; D014280:Triglycerides / Q000097:blood
D011933:Reagent Kits, Diagnostic; D014280:Triglycerides; D001663:Bilirubin