The requirement for adherent cells in the Fc fragment-induced proliferative response of murine spleen cells.
The proliferative response of mouse B lymphocytes induced by Fc fragments was found to be dependent upon an adherent cell population. The adherent cell is esterase positive, irradiation resistant, and not susceptible to lysis by anti-thymus serum and complement. The mechanism(s) by which Fc fragments induce B-cell proliferation could be the result of the interaction of Fc with both B cells and adherent cells or with adherent cells which then release factors that trigger the B cells to proliferate. Spleen cells from the C3H/HeJ mouse were shown to be unable to respond to Fc fragments. The addition of adherent cells from either C3H/St or C3H/HeN mice to adherent cell depleted C3H/HeJ cells enabled them to respond to Fc, indicating the defect was in the adherent cell population.
D000818:Animals; D000961:Antilymphocyte Serum; D002448:Cell Adhesion; D003165:Complement System Proteins; D004731:Endotoxins; D007141:Immunoglobulin Fc Fragments / Q000302:isolation & purification; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009194:Myeloma Proteins / Q000032:analysis; D013154:Spleen / Q000166:cytology*
D000961:Antilymphocyte Serum; D004731:Endotoxins; D007141:Immunoglobulin Fc Fragments; D009194:Myeloma Proteins; D003165:Complement System Proteins