Neuraminidase treatment reveals sialic acid differences in certain genetic variants of the Gc system (vitamin-D-binding protein).
Common and rare Gc phenotypes were analyzed by agarose gel electrophoresis immunofixation and by isoelectric focusing immunofixation before and after neuraminidase treatment. There is a difference between Gc types with double-band and with single-band patterns. Neuraminidase affects only Gc types with a double-band pattern: The double-band pattern is reduced to a single-band pattern. The anodal band disappears after removal of sialic acid by enzymatic cleavage; the cathodal band remains unchanged in its position and becomes stronger. Single-band Gc phenotypes remain unaltered after neuraminidase treatment.
D002352:Carrier Proteins / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D009439:Neuraminidase / Q000494:pharmacology*; D010641:Phenotype; D012794:Sialic Acids / Q000032:analysis*; D014807:Vitamin D / Q000378:metabolism*
D002352:Carrier Proteins; D012794:Sialic Acids; D014807:Vitamin D; D009439:Neuraminidase