Macromolecular synthesis in mitochondria isolated from different regions of developing rat brain.
DNA, RNA, and protein synthesis in mitochondria isolated from cerebral hemispheres, brain stem, and cerebellum of 10- and 30-day-old rats was measured. Synthesis of different macromolecules was affected by the respective mitochondrial specific inhibitors, showing a good level of purity of mitochondrial preparations. DNA and protein synthesis in 10-day-old rats was about 70% higher than in 30-day-old animals. In contrast, RNA synthesis did not decrease with age in all the regions examined.
D000375:Aging; D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000096:biosynthesis*; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D014018:Tissue Distribution
D004272:DNA, Mitochondrial; D009419:Nerve Tissue Proteins; D012313:RNA