Electrophoretic variant of fetal intestinal alkaline phosphatase in a patient with cirrhotic liver.
Alkaline phosphatases, which had a unique electrophoretic mobility on polyacrylamide gel electrophoresis, were found in hepatic tissue of a patient with liver cirrhosis. Enzymic and immunological properties of the enzymes examined on electropherogram were similar to those of a fetal intestinal-type alkaline phosphatase in hepatoma with respect to sensitivity to amino acids, heat stability, sensitivity to sodium dodecyl sulfate, and reactivity to anti-intestinal alkaline phosphatase antiserum. The enzymes seem to be a variant of a fetal intestinal alkaline phosphatase. The significance of occurrence of the enzymes in cirrhotic liver is discussed.
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000037:antagonists & inhibitors; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus; D006709:Homoarginine / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007422:Intestines / Q000201:enzymology*; D007527:Isoenzymes / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D008103:Liver Cirrhosis / Q000201:enzymology*; D009439:Neuraminidase / Q000494:pharmacology; D010649:Phenylalanine / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D007527:Isoenzymes; D006709:Homoarginine; D012967:Sodium Dodecyl Sulfate; D010649:Phenylalanine; D000469:Alkaline Phosphatase; D009439:Neuraminidase