[Effect of a saccharose diet on the enzymatic activity and the metabolite content from carbohydrate metabolism in the saliva of rats of varying age].
In rats, starting from a month or three-months age, stayed for 2 months on a diet with 54 per cent of saccharose in the saliva the activity of hexokinase, aldolase, malate-dehydrogenase, sorbitol-dehydrogenase, glutamate-dehydrogenase, glucose-6-phosphate-dehydrogenase and the content of lactate, pyruvate and glucose were determined. In the activity of significant differences of enzymes and carbohydrate metabolites in the saliva of three- and five-months old rats were not disclosed. Keeping of the one-month rats for 2 months on the saccharose diet increased the activity of enzymes (except for malate-dehydrogenase) and raised the amount of lactate and pyruvate. In five-month rats receiving the saccharose ration starting from three-month age a tendency towars a rising activity of hexoninase and to a falling malate-dehidrogenase activity were noted. The activity of other enzymes and the lactate level remained unchageed. In young rats given a saccharose diet the presence of an enzymatic shift toward intensification of the anaerobic glycolysis was confirmed by change in the isofermentative spectrum of the lactate-dehydrogenase accompanied by a drop of the total amount of aerobic isoenzymes LDG1, LDG2 and also by an excess accumulation of pyruvate and lactate.
D000375:Aging; D000818:Animals; D050260:Carbohydrate Metabolism; D003731:Dental Caries / Q000378:metabolism; D004040:Dietary Carbohydrates; D004789:Enzyme Activation / Q000187:drug effects; D051381:Rats; D012463:Saliva / Q000187:drug effects / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology*
D004040:Dietary Carbohydrates; D013395:Sucrose
Vliianie sakharoznoĭ diety na aktivnost' fermentov i soderzhanie metabolitov uglevodnogo obmena v sliune krys raznogo vozrasta.