Fluorescent retrograde double labeling: axonal branching in the ascending raphe and nigral projections.
Red fluorescent Evans blue and blue fluorescent DAPI-primuline were injected into the anterior-medial and lateral-caudal forebrains, respectively, of the same rats. Separate clusters of cells labeled by retrograde transport were observed in the substantia nigra, while in the dorsal raphe many cells were double-labeled. Thus, single raphe cells send divergent axon collaterals to widespread forebrain areas.
D000818:Animals; D001931:Brain Mapping / Q000379:methods; D001933:Brain Stem / Q000166:cytology*; D002540:Cerebral Cortex / Q000166:cytology*; D005456:Fluorescent Dyes; D009434:Neural Pathways / Q000166:cytology; D011903:Raphe Nuclei / Q000166:cytology*; D051381:Rats; D013378:Substantia Nigra / Q000166:cytology*
D005456:Fluorescent Dyes