Cytotoxic effects of tris(2,3-dibromopropyl)phosphate and 2,4-diaminoanisole.
The flame retardant tris(2,3-dibromopropyl)phosphate (Tris-BP) and the hair-dye component 2,4-diaminoanisole (2,4-DAA) were studied by possible cytotoxic effects in rat hepatoma cells grown in culture and in suspensions of isolated rat hepatocytes. Cell growth of Reuber cells was inhibited by 50% at 50 microgram/ml Tris-BP and 20 microgram/ml 2,4-DAA, respectively. At 200 microgram/ml Tris-BP protein synthesis in Reuber cells was reduced by 40%, whereas 50% inhibition of protein synthesis in isolated hepatocytes was seen at 100 microgram/ml. IC50 of 2,4-DAA with respect to protein synthesis was found at 400 microgram/ml in Reuber cells and at 3600 microgram/ml in MH1C1 cells, whereas in the isolated hepatocytes IC50 was 650 microgram/ml. DNA synthesis was inhibited by 50% at 225 microgram/ml Tris-BP in Reuber cells. At 500 microgram/ml 2,4-DAA DNA synthesis in Reuber and MH1C1 cells was inhibited by more than 80%.
D000818:Animals; D000840:Anisoles / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005411:Flame Retardants / Q000633:toxicity; D006202:Hair Dyes / Q000633:toxicity; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008114:Liver Neoplasms, Experimental / Q000473:pathology; D008297:Male; D009153:Mutagens; D010710:Phosphates / Q000633:toxicity*; D014176:Protein Biosynthesis; D051381:Rats
D000840:Anisoles; D005411:Flame Retardants; D006202:Hair Dyes; D009153:Mutagens; D010710:Phosphates; D004247:DNA