Anomalous oxygen uptake from isolated chloroplasts inhibited in photosystem II and without external electron donors.
Light induced modulated signal of oxygen uptake by isolated chloroplasts in the presence of methyl viologen, when Photosystem II activity was inhibited and in the absence of any electron donors, was detected by a modulated oxygen Pt electrode, polarized negatively. Evidence is brought to show that an electrochemical process which takes place on the surface of the negatively polarized Pt-cathode produces an intermediate which serves as an electron donor to Photosystem I. Atempts to identify this intermediate show that it may be very probably the superoxide radical generated by the electrochemical reduction of oxygen which continuously diffuses from the external circulating medium to the electrode.
D002462:Cell Membrane / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D004237:Diuron / Q000494:pharmacology; D004579:Electron Transport; D006358:Hot Temperature; D007700:Kinetics; D010101:Oxygen Consumption / Q000187:drug effects; D010788:Photosynthesis / Q000187:drug effects; D010944:Plants
D004237:Diuron