Gas-liquid chromatographic measurement of lignocaine in small samples of canine myocardium after enzymatic digestion.
A method is described whereby lignocaine can be measured in small samples of canine myocardium. A proteolytic enzyme is used to digest the tissue, and the drug, together with an internal standard, is subsequently extracted into an organic solvent and measured by gas-liquid chromatography. The method is reproducible using tissue samples as small as 50 mg and appears to be suitable for the measurement of a number of drugs in small samples of tissue.
D000818:Animals; D002849:Chromatography, Gas; D004285:Dogs; D008012:Lidocaine / Q000032:analysis*; D008722:Methods; D009206:Myocardium / Q000032:analysis*; D010447:Peptide Hydrolases
D008012:Lidocaine; D010447:Peptide Hydrolases