title: Cromatin and core particles formed from the inner histones and synthetic polydeoxyribonucleotides of defined sequence.
abstract: Chicken erythrocyte inner histones (H2A, H2B, H3 and H4) were associated with the two complementary homopolymeric polydeoxyribonucleotides and the two alternating copolymeric polydeoxyribonucleotides. No evidence for formation of chromatin-like structures was obtained for the complexes with poly(dG) . poly(dC) or poly(dA) . poly(dT). Both poly (dGdC) . poly(dGdC) and poly(dAdT) . poly(dAdT) could be folded by histones to yield material digested by DNAase I to multiples of about 10 and by staphylococcal nuclease to 146 bp core particles. Due to the lack of sequence heterogeniety in the complex of histones with poly(dAdT) . poly(dAdT), core particles with remarkable fine structural detail are obtained. The internal organization of DNA in the AT-containing and GC-containing core particles appears not to be identical.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D003852:Deoxyribonucleoproteins; D004912:Erythrocytes / Q000032:analysis; D006657:Histones / Q000097:blood; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009698:Nucleoproteins; D011089:Polydeoxyribonucleotides; D011487:Protein Conformation; D013329:Structure-Activity Relationship
chemical_list: D002843:Chromatin; D003852:Deoxyribonucleoproteins; D006657:Histones; D009698:Nucleoproteins; D011089:Polydeoxyribonucleotides