[Preparation of monospecific antibodies to A-protein and an immunochemical study of its distribution in normal and tumorous tissues].
Monospecific antibodies against A-protein from rat liver cytosol were prepared. Complete nonidentity was established in comparison of the A-protein and ligandin, which was similar to A-protein in binding properties. Besides, A-protein found in cytosol of liver tissue was present in cytosol of small intestine. It was not found in the cytosol of other 17 tissues. A-protein appeared in ontogenesis earlier than ligandin: its concentration sharply increased in rat embryonal liver within 16--18 day of gestation. Level of A-protein was decreased 2--4-fold in primary rat hepatomas. A-protein and ligandin are the isoforms of binding proteins, which characterize different steps of the differentiation of the hepatocytes.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D005260:Female; D005982:Glutathione Transferase / Q000276:immunology; D007421:Intestine, Small / Q000378:metabolism; D008099:Liver / Q000276:immunology / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental / Q000378:metabolism*; D011506:Proteins / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D014018:Tissue Distribution
D000906:Antibodies; D011506:Proteins; D005982:Glutathione Transferase
Poluchenie monospetsificheskikh antitel k A-belku i immunokhimicheskoe izuchenie ego raspredeleniia v normal'nykh i opukholevykh tkaniakh.