Micromethods for analysis of lipids in endomyocardial biopsy specimens.
Triglycerides and phospholipids extracted from endomyocardial biopsy specimens were quantitated with sensitive photometric methods and the fatty acid composition of phospholipids was determined after separation of the methyl esters by gas chromatography. The degree of triglyceride accumulation was expressed as the molar ratio triglyceride/phospholipid since in most cases the phospholipid concentration is relatively constant. The representativity of the analysis of endomyocardial biopsy was investigated by comparing lipid analyses of large and small myocardial specimens obtained at necropsy. The methods described may be valuable tools in studies on metabolic disturbances in different cardiomyophaties.
D001706:Biopsy; D009202:Cardiomyopathies / Q000175:diagnosis* / Q000378:metabolism; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D008832:Microchemistry; D009206:Myocardium / Q000032:analysis* / Q000473:pathology; D009929:Organ Size; D010743:Phospholipids / Q000032:analysis*; D010783:Photometry / Q000379:methods*; D014280:Triglycerides / Q000032:analysis*
D005227:Fatty Acids; D010743:Phospholipids; D014280:Triglycerides