Chemical evidence for production of hydroxyl radicals during microsomal electron transfer.
Rat liver microsomes generate methane from dimethyl sulfoxide and ethylene from either methional or 2-keto-4-thiomethylbutyric acid during electron transfer initiated by reduced nicotinamide-adenine dinucleotide phosphate (NADPH). Hydrocarbon gas production is suppressed by hydroxyl radical scavenging agents. Azide, an inhibitor of catalase, augments the production of hydrocarbon gases. These observations constitute chemical evidence for the generation of hydroxyl radicals by microsomes.
D000438:Alcohols / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000378:metabolism; D004579:Electron Transport; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D006878:Hydroxides; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats
D000438:Alcohols; D005609:Free Radicals; D006878:Hydroxides; D006861:Hydrogen Peroxide; D002374:Catalase