High oxygen pressure inhibits DNA synthesis in mitogen-activated lymphocytes.
Peripheral sheep lymphocytes, exposed to the mitogenic lectin phytohemagglutinin do not incorporate radiolabelled thymidine in cultures with a gas phase high in oxygen. Cells kept under nitrogen incorporate thymidine to the same extent or better than cells grown under air. The inhibition is proportional to the oxygen content of the gas phase. The inhibition is relieved if oxygen is replaced by air after some time but the onset of DNA synthesis is delayed correspondingly. It is suggested that oxygen may have a regulatory function on the onset of DNA synthesis.
D000818:Animals; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis*; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011312:Pressure; D012756:Sheep
D010835:Phytohemagglutinins; D004247:DNA; D010100:Oxygen