Use of electrophoresis and gel filtration to characterize human pituitary somatotropin preparations after storage.
Fresh and stored preparations of human pituitary somatotropin examined by different polyacrylamide gel electrophoresis techniques and by gel exclusion chromatography (Ultrogel) clearly demonstrated that the hormone undergoes a structural alteration during storage for 3 years. The formed aggregates were readily quantitated from the gel filtration profile and from polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate (SDS-PAGE). According to gel filtration the amount of aggregates in fresh and stored hormone was determined to be 2 and 10%, respectively, while SDS-PAGE indicated 6 and 14%.
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D013006:Growth Hormone / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D008968:Molecular Conformation; D008970:Molecular Weight; D011092:Polyethylene Glycols; D011309:Preservation, Biological; D013997:Time Factors
D011092:Polyethylene Glycols; D013006:Growth Hormone