title: Plasmid-determined 2-hydroxypyridine utilization by Arthrobacter crystallopoietes.
abstract: Arthrobacter crystallopoietes has the ability to utilize 2-hydroxypyridine (2-HP) as a source of carbon and nitrogen and forms a blue extracellular pigment when grown in the presence of 2-HP. Ultracentrifugal analyses of pigment producing (Pig+) and pigment nonproducing (Pig-) strains of A. crystallopoietes revealed the presence of plasmid material in both strains. Recovery of plasmid DNA from Pig+ strains is two or three times greater than from Pig- strains. The molecular weight of plasmid DNA recovered from Pig+ strains (62 Mdaltons) is slightly higher than the molecular weight of plasmid DNA from Pig- strains. Consistent with the characterization of plasmid DNA from the two strains is that Pig+ strains contain a 63-Mdalton plasmid encoding 2-HP utilization as well as a cryptic plasmid of very nearly equal molecular weight. Pig- strains contain only the cryptic plasmid.
mesh_terms: D001173:Arthrobacter / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004270:DNA, Circular / Q000032:analysis; D008970:Molecular Weight; D009154:Mutation; D010860:Pigments, Biological / Q000096:biosynthesis; D010957:Plasmids; D011725:Pyridines / Q000378:metabolism*; D011728:Pyridones
chemical_list: D004269:DNA, Bacterial; D004270:DNA, Circular; D010860:Pigments, Biological; D011725:Pyridines; D011728:Pyridones; C017679:2-hydroxypyridine