Specific radioimmunoassay of amitriptyline and nortriptyline.
1 Antisera to nortriptyline were prepared by immunizing rabbits with N-succinylnortriptyline--bovine serum albumin conjugate. 2 A sensitive radioimmunoassay has been developed for the tricyclic antidepressants amitriptyline and nortriptyline. 3 amitriptyline and nortriptyline are separated from each other and from interfering metabolites before assay. 4 Using [3H]-imipramine and [3H]-succinylnortriptyline as tracers the radioimmunoassay can measure amitriptyline and nortriptyline levels down to 2--3 ng/ml using 0.05 ml plasma sample. 5 Agreement between the radioimmunoassay and a gas-chromatographic assay was excellent for both amitriptyline and nortriptyline.
D000639:Amitriptyline / Q000097:blood* / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002417:Cattle; D002849:Chromatography, Gas; D008722:Methods; D009661:Nortriptyline / Q000097:blood* / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay
D000639:Amitriptyline; D009661:Nortriptyline