Construction of a site-specific, deletion-frameshift mutation in an essential gene of bacteriophage phiX174.
By in vitro methods, we have deleted 80 nucleotides from a preselected site in the coding sequence of gene G of bacteriophage phiX174. This mutant (Gdelta/FS ZH1) also carries a--2 frameshift. The mutation is lethal, but mutant virus can be stably maintained on host strains bearing plasmids carrying phiX gene G.
D001483:Base Sequence; D002872:Chromosome Deletion; D003062:Codon; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005796:Genes; D005804:Genes, Lethal; D005814:Genes, Viral; D009154:Mutation; D010957:Plasmids; D014158:Transcription, Genetic; D014779:Virus Replication
D003062:Codon; D004279:DNA, Viral