title: Uptake and loss of tears from filter paper discs employed in lysozyme tests.
abstract: Aspects of the use of filter paper discs in collecting tear samples for lysozyme tests were studied by serial weighings of bijoux bottles and tear-moistened discs. Blotted discs carried from 1.9 to 3.6 mg tears, but for practical purposes no deposit was left in the bottle. Unblotted discs carried a greater quantity of tear fluid, but the majority deposited a proportion of this in the bottle, and a correcting factor is therefore proposed for the lysozyme assay technique of Mackie and Seal. Substantial vaporisation of tears occurred within the bottles.
mesh_terms: D000293:Adolescent; D000328:Adult; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D008875:Middle Aged; D009113:Muramidase / Q000032:analysis*; D013048:Specimen Handling / Q000379:methods*; D013666:Tears / Q000201:enzymology*
chemical_list: D009113:Muramidase