Comparison of the protective action of glutathione and cysteamine on radiation-induced mitotic delay in cultured S-5 cells.
The protective effect of glutathione (GSH) and cysteamine (MEA) on radiation-induced mitotic delay in cultured mammalian L-5 cells was studied. Cells treated with 20 mM of GSH during irradiation with 2 Gy (200 rad) showed faster recovery of the mitotic index than control cells irradiated without chemical treatment; however, GSH had no effect on mitotic delay time. Inhibition of mitosis was observed with 80, 100, and 120 mM of GSH. Cells treated with 5 mM of MEA during irradiation also showed faster recovery of the mitotic index than the controls, but in addition the delay time was shortened. Progression of G2-phase cells treated with 5-fluorouracil to mitosis after irradiation was protected by MEA but not by GSH. Progression of S-phase cells labeled with 3H-thymidine to mitosis was accelerated by both agents during irradiation.
D000818:Animals; D001345:Autoradiography; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D003543:Cysteamine / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology*; D007399:Interphase / Q000187:drug effects / Q000528:radiation effects; D007739:L Cells / Q000187:drug effects / Q000528:radiation effects*; D051379:Mice; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects*; D008940:Mitotic Index / Q000187:drug effects / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000494:pharmacology; D013997:Time Factors
D011837:Radiation-Protective Agents; D003543:Cysteamine; D005978:Glutathione