Studies on the terminal stages of immune hemolysis. IV. Effect of metal salts.
The inhibitory effect of metal salts on the formation and transformation of E to ghosts was studied. Ferrous and ferric salts inhibited the binding of C9 to EAC1-8 cells but had no other inhibitory role in the reaction. Uranly, copper, and zinc salts inhibited the transformation of the EAC1-9inserted intermediate to E ghosts. This inhibition occurred at a stage prior to detectable damage as measured by 86Rb or hemoglobin release and at a step prior to that inhibitable by high molarity EDTA. Consequently a further step in the reaction sequence of E to ghosts was identified. The reaction sequence leading from EAC1-9 to ghosts can be summarized as follows: formula: (see text).
D000818:Animals; D002417:Cattle; D003171:Complement Pathway, Classical; D003300:Copper / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000276:immunology; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D008670:Metals / Q000494:pharmacology*; D010618:Phenanthrolines / Q000378:metabolism; D011817:Rabbits; D012492:Salts / Q000494:pharmacology; D012756:Sheep; D013997:Time Factors; D014501:Uranium / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D006454:Hemoglobins; D008670:Metals; D010618:Phenanthrolines; D012492:Salts; D014501:Uranium; D003300:Copper; D015032:Zinc