An unexpected albumin-bilirubin band in cellulose acetate electrophoresis for alkaline phosphatase isoenzymes.
In 4 out of 202 consecutive electrophoretic patterns for serum alkaline phosphatase isoenzymes, an extra band was observed in the albumin zone. Subsequent heat inactivation tests and comparison studies with an albumin-based bilirubin standard indicated that the band was due to staining of an albumin-bilirubin complex. The band should not be mistaken for an isoenzyme of alkaline phosphatase.
D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis / Q000097:blood*; D001663:Bilirubin / Q000097:blood*; D001797:Blood Protein Electrophoresis; D002779:Cholestasis / Q000097:blood; D004586:Electrophoresis; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000097:blood*; D008107:Liver Diseases / Q000097:blood; D008875:Middle Aged; D012709:Serum Albumin / Q000032:analysis*
D007527:Isoenzymes; D012709:Serum Albumin; D000469:Alkaline Phosphatase; D001663:Bilirubin