[Radiosensitivity of colony-forming units of dog bone marrow in agar cultures].
Radiosensitivity of the bone-marrow colony-forming cells was determined by a modified method of hemopoietic cells cloning in vivo in semihard agar gel in diffusion chambers. Do for the commited precursor cells of granulopoiesis (CFUc) was 144 +/- 14.8 rad (n = 0.8), and Do for the precursor cells forming "stellate" colonies of fibroblast-like cells (PFUf) was 468 +/- 35.8 rad (n == 0.9). A conclusion was drawn that PFUf were referred to the class of stromal precursors of the hemopoietic organs. This system can be applied for a simultaneous study of the hemopoietic and stromal precursor cells in dogs.
D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D002588:Cesium Radioisotopes; D004285:Dogs; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D006412:Hematopoietic Stem Cells / Q000528:radiation effects*; D066298:In Vitro Techniques; D008297:Male
D002588:Cesium Radioisotopes
Radiochuvstvitel'nost' kolonieobrazuiushchikh edinits kostnogo mozga sobak v agarovykh kul'turakh.