Radioimmunoassay for 2-methoxyoestrone in human plasma.
A bovine serum albumin conjugate of 2-methoxyoestrone was used for the preparation of highly specific antibodies in rabbits. Cross-reactivity for catecholoestrogens and monophenolic steroids was below 0.3%. Only 2-methoxyoestradiol cross-reacted with 44%. An assay procedure for the determination of unconjugated and conjugated 2-methoxyoestrone in human plasma is described. The following mean plasma concentrations (pg/ml) were found (unconjugated/conjugated): children 61/1130, young men 74/1320, elderly men 109/1260, cycling women 131/1040, post-menopausal women 102/1420, and pregnant women 3980/5850.
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002854:Chromatography, Paper; D003429:Cross Reactions; D004970:Estrone / Q000031:analogs & derivatives* / Q000097:blood / Q000276:immunology; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods*
D007106:Immune Sera; D004970:Estrone