Analytical peptide mapping by high performance liquid chromatography. Application to intestinal calcium-binding proteins.
Peptide mapping of underivatized tryptic digests of bovine and chick intestinal calcium-binding proteins has been accomplished by high performance liquid chromatography (HPLC). High precision analysis of nanomolar quantities of peptides were achieved in less than 1 h (recycle time). Peak resolution and definition are superior compared to conventional techniques and recoveries of both small (4-residue) hydrophilic and large (30-residue) hydrophobic peptides are excellent. The total amino acid composition of the bovine intestinal calcium-binding protein has been accounted for on the basis of two tryptic maps of 20 microgram of protein each.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007422:Intestines / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D064030:S100 Calcium Binding Protein G; D014357:Trypsin
D000596:Amino Acids; D002352:Carrier Proteins; D010446:Peptide Fragments; D064030:S100 Calcium Binding Protein G; D014357:Trypsin