The use of the potassium pyroantimonate-osmium method as a means of identifying and localizing calcium at the ultrastructural level in the cells of calcifying systems.
Potassium pyroantimonate-osmium has been used to localize calcium as an electron dense precipitate in the odontoblast, ameloblast and early hypertrophic chondrocytes of the mandibular condylar growth cartilage. The precipitate exhibited a specific association with various cell organelles. The presence of calcium in the precipitates was confirmed using energy dispersive analysis by x-rays. It is suggested that this K-pyroantimonate method provides an accurate technique for the investigation of the subcellular localization of calcium in calcifying systems.
D000818:Animals; D000965:Antimony; D002118:Calcium / Q000378:metabolism*; D002356:Cartilage / Q000378:metabolism* / Q000648:ultrastructure; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009992:Osmium; D011188:Potassium; D051381:Rats
C009002:potassium antimonate; D009992:Osmium; D000965:Antimony; D011188:Potassium; D002118:Calcium