Liposomes and local hyperthermia: selective delivery of methotrexate to heated tumors.
Liposomes with phase transitions a few degrees above physiological temperature delivered more than four times as much methotrexate to murine tumors heated to 42 degrees C as to unheated control tumors. Most of the accumulated drug appeared to be intracellular and bound to dihydrofolate reductase, the enzyme blocked by methotrexate in its role as an antineoplastic agent.
D000818:Animals; D006358:Hot Temperature; D008081:Liposomes / Q000627:therapeutic use*; D008297:Male; D008657:Metabolic Clearance Rate; D008727:Methotrexate / Q000008:administration & dosage* / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D010743:Phospholipids; D013329:Structure-Activity Relationship
D008081:Liposomes; D010743:Phospholipids; D008727:Methotrexate