Flow cytometry: a high-resolution instrument for everyone.
A new flow configuration for flow cytometry has been devised in which a flat, laminar stream of water, containing the stained cells in a narrow sector, is formed on a microscope cover slip by a pressurized jet of water directed onto the glass at low angle. The stream of cells is viewed by means of a fluorescence microscope with incident illumination and a pulse photometer. Coupled to a multichannel pulse height analyzer, the instrument constitutes a stable and easy-to-operate flow cytometer with a resolution equal to or better than a coefficient of variance of 1.4 percent in measurements of cellular DNA.
D000818:Animals; D002469:Cell Separation / Q000295:instrumentation*; D004247:DNA / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D051381:Rats; D013950:Thymus Gland / Q000166:cytology
D004247:DNA