Inhibition of vaccinia virus growth by zinc ions: effects on early RNA and thymidine kinase synthesis.
Accumulation of thymidine kinase activity in vaccinia virus-infected cells was severely inhibited by zinc ions if the drug was added within 1 h postinfection. If added later, zinc ions had no effect on the enzyme synthesis. A fraction of RNA which is normally synthesized in infected cells, was missing from a proper part of the gradient if the cells were treated with zinc ions within 1 h postinfection (as has been shown by cosedimentation of pulse-labeled RNAs in isokinetic gradients). It is suggested that a transcriptional (or posttranscriptional) step is involved in zinc-caused inhibition of vaccinia virus growth.
D004279:DNA, Viral / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis*; D013937:Thymidine Kinase / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects; D014616:Vaccinia virus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis; D015032:Zinc / Q000494:pharmacology*
D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D013937:Thymidine Kinase; D015032:Zinc