Human natural cell-mediated cytotoxicity against fetal fibroblasts. IV. Comparison of cytotoxic activity with antibody-dependent cell-mediated cytotoxicity.
The dependence of human natural killer (NK) cell activity on antibodies was investigated. Absorption of the culture medium with target cells, trypsinization of the effector cells followed by a brief recovery, and incubation of the effector cells in serum-free nutrient medium did not affect natural killer cell activity against fetal fibroblasts. No soluble mediator in the nutrient media of effector cell--fibroblast co-cultures could be demonstrated. It was therefore concluded that antibodies are not involved in the cytolytic activity of natural killer cells. However, competition data and the activity of isolated NK cells against antibody-coated target cells suggested overlapping between the effector cells mediating natural and antibody-dependent cell-mediated cytotoxicity.
D000920:Antibody-Dependent Cell Cytotoxicity; D002478:Cells, Cultured; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D005347:Fibroblasts / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D007694:Killer Cells, Natural / Q000276:immunology*; D008168:Lung / Q000166:cytology / Q000196:embryology; D014357:Trypsin / Q000494:pharmacology
D003470:Culture Media; D007106:Immune Sera; D014357:Trypsin