Methods for binding cells to plastic: application to a solid-phase radioimmunoassay for cell-surface antigens.
Two methods are described for attaching cells to plastic plates such that they may be used for antibody binding assays. In the first method, lymphoid cells or erythrocytes were attached to the wells of plastic plates using glutaraldehyde. This resulted in monolayers of fixed cells which retained surface antigens and were stable to storage. The second method involved binding of unfixed cells to the plastic surface by means of antibodies non-specifically adsorbed to the plate. Both methods resulted in cell layers which remained attached to the plate during the washing and incubation procedures of a radioimmunoassay. The cell layers were shown to be suitable for screening the product of hybrid cell lines for the presence of monoclonal antibodies to cell-surface antigens.
D000818:Animals; D000918:Antibody Specificity; D000954:Antigens, Surface; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001666:Binding Sites, Antibody; D002448:Cell Adhesion; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011863:Radioimmunoassay / Q000379:methods
D000954:Antigens, Surface; D000961:Antilymphocyte Serum; D007106:Immune Sera