title: Improved electron-capture GLC determination of alprenolol and oxprenolol in serum using a wall-coated open tubular column.
abstract: An accurate, rapid, and sensitive GLC determination of alprenolol and oxprenolol in serum is described. This method combines an electron-capture detector with a wall-coated open tubular column. The lowest detectable amount of the halogenated (heptafluoroacyl) derivatives of alprenolol and oxprenolol is 2 pg. The high resolving power and the rapid elution time of the wall-coated open tubular column dramatically improve the performance (accuracy and sensitivity) of the conventional electron-capture detector and packed column system in these determinations. This method allows the use of small volumes of sample (100-200 microliter), does not require redistilled reagents, and has a simplified extraction procedure.
mesh_terms: D000526:Alprenolol / Q000097:blood*; D000818:Animals; D002849:Chromatography, Gas / Q000295:instrumentation; D004285:Dogs; D006801:Humans; D008722:Methods; D010096:Oxprenolol / Q000097:blood*
chemical_list: D010096:Oxprenolol; D000526:Alprenolol