Murine primitive neuroectodermal tumor: nucleolar isolation and RNA polymerase inhibition.
A nucleolar isolation procedure was developed for the murine primitive neuroectodermal tumor originally induced with methylcholanthrene. This procedure utilizes sonication for breakage of the nuclei at a magnesium concentration which disperses the extranucleolar chromatin. The nucleoli retain RNA synthetic activity which is inhibited to about 90% by actinomycin D and the chemotherapeutic drug adriamycin.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002458:Cell Fractionation; D002466:Cell Nucleolus / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D003609:Dactinomycin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D004806:Ependymoma / Q000201:enzymology / Q000648:ultrastructure*; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000201:enzymology / Q000648:ultrastructure; D012318:RNA Polymerase I / Q000037:antagonists & inhibitors*
D000546:Amanitins; D003609:Dactinomycin; D004317:Doxorubicin; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I; D008274:Magnesium