Lack of oestradiol-DNA binding in mouse embryo cell cultures or following rat-liver microsomal metabolism.
Oestradiol-17beta was compared to benzo[a]pyrene (BP) for its ability to bind to the DNA of mouse embryo cells in culture or to DNA added to a rat liver microsomal incubation mixture. No significant binding was found in embryo cells (at least 100-fold less than for BP). Microsomal incubation resulted in apparent oestradiol binding to a reisolated DNA-containing complex, but most of this was lost when the DNA was freed of RNA and protein.
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D004622:Embryo, Mammalian; D004958:Estradiol / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats
D001580:Benzopyrenes; D004958:Estradiol; D004247:DNA