Isolation of stable aggregates of IgG by zonal ultracentrifugation in sucrose gradients containing albumin.
Soluble heat aggregates of [125I]IgG (A-IgG) were prepared and separated by gel filtration or by zonal ultracentrifugation, and fractions containing different size aggregates were then analyzed by reultracentrifugation. A-IgG formed single narrow peaks with constant S rates when isolated, stored and analyzed in solution containing 0.5% serum albumin. Thus, stable homogeneous aggregates of IgG of known size can be simply prepared and should prove useful both as a model for immune complexes of specified sizes and as a standard for immune complex assays.
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D002850:Chromatography, Gel; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D051379:Mice; D011817:Rabbits; D051381:Rats; D012710:Serum Albumin, Bovine; D013997:Time Factors; D014461:Ultracentrifugation
D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine