The preparation of 125I-labelled bile acid ligands for use in the radioimmunoassay of bile acids.
A general method for the preparation of 125I-labelled bile acid-histamine or 125I-labelled bile acid-tyramine conjugates is presented. The method is simple, quick and produces ligands in good yield (30%). The characteristics of a radioimmunoassay for conjugated chenodeoxycholic acid, based on an 125I-labelled ligand prepared by the method, are also described. The assay produced values for fasting serum concentrations of conjugated chenodeoxycholic acid that agree well with previous data.
D001647:Bile Acids and Salts / Q000032:analysis*; D002635:Chenodeoxycholic Acid / Q000032:analysis; D003429:Cross Reactions; D006000:Glycocholic Acid / Q000032:analysis; D006632:Histamine; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D011863:Radioimmunoassay / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D014439:Tyramine
D001647:Bile Acids and Salts; D007457:Iodine Radioisotopes; D002635:Chenodeoxycholic Acid; D006632:Histamine; D006000:Glycocholic Acid; D014439:Tyramine