title: [Effect of tocopherol and protein deficiency on the activity of enzymes and their isoenzymatic spectra in rat testes].
abstract: The symptoms of E-avitaminosis against protein deficiency in the ration become manifest much earlier, i. e. in 12--16 weeks of the experiment instead of 24--36 weeks with the primary-deficient ration. This was marked by the development of the creatinuria, the falling blood serum and testes tissue tocopherol, a higher peroxidized erythrocytes hemolysis, the animals demonstrating a disturbed generative function. In the testes of the E-avitaminotic rats an appreaciably increased total activity of glucose-6-phosphate-dehydrogenase (G-6-PDG) and of the acid phosphatase (AP) was demonstrated, whereas the LDG remained essentially unchanged. An investigation of their isoenzymatic spectra revealed a considerable activity of the basic 5 G-6-PDG fractions with the appearance of additional ones, along with the disappearance of the specific LDG fraction--X-2 and a drastic fall in the activity of the specific fraction for the testes--LDG-X-1, with concurrent rise in the activity of the 5 basic fractions. In the isoenzymatic AP spectrum disappeared the AP-3 fraction of the degenerating testes tissues and a redistribution of the activity between the fractions took place.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D004789:Enzyme Activation; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011488:Protein Deficiency / Q000201:enzymology*; D051381:Rats; D013081:Sperm Motility / Q000187:drug effects; D013737:Testis / Q000201:enzymology*; D013997:Time Factors; D014811:Vitamin E Deficiency / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D000135:Acid Phosphatase
vernacular_title: Vliianie nedostatochnosti tokoferola i belka na aktivnost' nekotorykh fermentov i ikh izofermentnye spektry semennikov krys.