The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity.
We have compared the chromatin structure in the active and inactive states at loci encoding the major heat shock protein in Drosophila. DNAase I and micrococcal nuclease were used as probes of higher order organization and nucleosomal integrity. Such integrity is gauged here by the characteristic pattern of discrete DNA fragments produced at specific chromosomal loci by nucleolytic cleavage. The specific fragment patterns are visualized by gel electrophoresis, Southern blotting onto nitrocellulose sheets, hybridization with 32P-labeled cloned DNA containing the heat shock genes and autoradiography. Using this criterion, a disruption in nucleosomal and possibly in higher order organization are observed as indicated by a relative loss or smearing of the characteristic discrete DNA fragment patterns from the heat shock loci in the active state. The fragment patterns are restored when cells are allowed to recover from heat shock and these loci return to the inactive state.
D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000648:ultrastructure*; D004274:DNA, Recombinant; D003851:Deoxyribonucleases / Q000378:metabolism; D004330:Drosophila / Q000235:genetics*; D005796:Genes; D006358:Hot Temperature; D008836:Micrococcal Nuclease / Q000378:metabolism; D009693:Nucleic Acid Hybridization
D002843:Chromatin; D004274:DNA, Recombinant; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease