The use of a digital oscillator densimeter for the determination of reaction rates of bacterial proteases.
A digital oscillator densimeter technique for measuring the density of casein solutions at different concentrations, temperatures and pH values was used. The technique was also used to compare the rates of protease-catalysed reactions of two bacterial proteases using casein as a substrate. Enzyme activities using reaction rates of proteases determined by this method were compared with a standard method for protease activity.
D001407:Bacillus / Q000201:enzymology; D002364:Caseins; D003720:Densitometry / Q000379:methods; D007700:Kinetics; D010447:Peptide Hydrolases / Q000378:metabolism*
D002364:Caseins; D010447:Peptide Hydrolases