title: Trypsinization frequency and loss of proliferative capacity in WI-38 cells.
abstract: The effect of the trypsinization procedure employed in routine serial subcultivation on the proliferative life span of WI-38 cells was studied by varying the frequency of trypsinization and by using a nonenzymatic subcultivation technique. The results indicate that recurrent trypsinization does not contribute to loss of proliferative capacity and may, in fact, delay this loss to some degree.
mesh_terms: D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D006801:Humans; D014357:Trypsin / Q000494:pharmacology*
chemical_list: D014357:Trypsin