title: Phosphorylation of the 20,000-dalton light chain of myosin of intact arterial smooth muscle in rest and in contraction.
abstract: The 20,000-dalton myosin light chain of intact pig carotid arteries was found to be rapidly labeled when the arterial muscle was incubated in physiological salt solution at 37 degrees C containing [32P]orthophosphate. Light chain phosphorylation in the intact muscle had a marked requirement for Ca2+ and was dependent upon the passive tension applied to the muscle. Norepinephrine- or KCl-induced contractures were associated with concomitant increases in light chain phosphorylation.
mesh_terms: D000205:Actomyosin / Q000378:metabolism; D000818:Animals; D002339:Carotid Arteries / Q000378:metabolism*; D008970:Molecular Weight; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D010766:Phosphorylation; D013552:Swine
chemical_list: D000205:Actomyosin; D009218:Myosins