Identification of the N-ethylmaleimide reactive protein of the mitochondrial phosphate transporter.
The mitochondrial phosphate carrier is inhibited by the SH reagents p-(hydroxymercuri)benzoate and N-ethylmaleimide. Based on an analysis utilizing dodecyl sulfate-polyacrylamide gels, an SH-containing 32 000-dalton protein has been identified as a component of the phosphate carrier system. Two other N-[3H]ethylmaleimide-labeled proteins of the inner mitochondrial membrane have been eliminated from this role [Wholrab, H., & Greaney, J., Jr. (1978) Biochim. Biophys. Acta 503, 425] on the basis that band IV (45,000 daltons) is absent from heart sonic submitochondrial particles and band VII (6 500 daltons) does not react with p-(hydroxymercuri)benzoate. The mobility of the 32 000-dalton protein (0.43) is lower than that of the gamma subunit of the mitochondrial ATPase (0.46) and the carboxyatractyloside binding protein (0.48) on 12.5% dodecyl sulfate-polyacrylamide gels. In these flight muscle mitochondria, 0.87 nmol of N-[3H]ethylmaleimide per nmol of cytochrome a is bound to the 32,000-dalton protein.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D004175:Diptera; D005033:Ethylmaleimide / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000378:metabolism; D008933:Mitochondrial Swelling; D008970:Molecular Weight; D010710:Phosphates / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D013367:Submitochondrial Particles / Q000378:metabolism
D002352:Carrier Proteins; D008565:Membrane Proteins; D010710:Phosphates; D005033:Ethylmaleimide