Electronic sorting of granulocyte precursor cells from stimulated bone marrow.
A commerical cell sorter was used to obtain preparations of cells in various stages of granulocyte development from rabbit marrows stimulated by inflammatory response. Marrow cells were fractionated on density gradients of Ficoll/Hypaque and each fraction sorted using light scatter. Trial and error selection of appropriate gradient fractions and light scatter windows allowed sorting of early (blast cells, promyelocytes), intermediate (myelocytes, metamyelocytes) and late stage (band cells, polys) granulocytes with enhanced purity.
D000818:Animals; D001854:Bone Marrow Cells; D002452:Cell Count; D002469:Cell Separation / Q000379:methods; D006098:Granulocytes / Q000166:cytology*; D008297:Male; D011817:Rabbits