Plasmid-mediated, transferable resistance to clindamycin and erythromycin in Bacteroides fragilis.
A clindamycin- and erythromycin-resistant strain of Bacteroides fragilis, TMP10, that had been isolated from a blood culture transferred its drug resistance in a Millipore mating procedure to another strain of B. fragilis, TM2000, and to a strain of Bacteroides thetaiotaomicron. Donor strain TMP10 contained numerous species of extrachromosomal DNA ranging from approximately 2 x 10(6) to 20 x 10(6) daltons in molecular weight, as revealed by agarose gel electrophoresis. Clindamycin- and erythromycin-resistant transcipient cells of B. fragilis strain TM2000 always contained at least two plasmid species with molecular weights of 2 x 10(6) and 20 x 10(6) daltons, respectively, although other plasmid species were also transferred.
D001439:Bacteroides / Q000187:drug effects; D001441:Bacteroides fragilis / Q000032:analysis / Q000187:drug effects*; D002847:Chromatography, Agarose; D002981:Clindamycin / Q000494:pharmacology*; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D004917:Erythromycin / Q000494:pharmacology*; D066298:In Vitro Techniques; D008970:Molecular Weight; D010957:Plasmids
D004269:DNA, Bacterial; D002981:Clindamycin; D004917:Erythromycin