title: Primary structure of the nucleic acid from the 1,4-alpha-glucan branching enzyme.
abstract: The primary structure of the nucleic acid from the branching enzyme 1,4-alpha-D-glucan: 1,4-alpha-D-glucan 6-alpha-(1,4-alpha-glucano)-transferase (2.5-S RNA) isolated from rabbit muscles has been elucidated. The polyribonucleotide consists of 31 nucleotides; the unique features of the polyribonucleotide are the unusually high content of modified nucleotides (32%) and guanine residues (40%). Apparently 2.5-S RNA belongs to a class of nucleic acids unknown up to now. It is the first time that the structure of a nucleic acid component from a ribonucleoenzyme has been defined. This work is a preprequisite for gaining insight into the intimate activating effect of the poly-ribonucleotide on the enzyme action.
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme; D000818:Animals; D001483:Base Sequence; D005964:Glucosyltransferases; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology; D011687:Purines / Q000032:analysis; D011743:Pyrimidines / Q000032:analysis; D012313:RNA / Q000032:analysis; D011817:Rabbits; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis
chemical_list: D011687:Purines; D011743:Pyrimidines; D012265:Ribonucleotides; D012313:RNA; D005964:Glucosyltransferases; D015061:1,4-alpha-Glucan Branching Enzyme; D012260:Ribonucleases