title: Synthesis of giant HnRNA in the epidermal cells of Calliphora and the role of the ring gland.
abstract: The appearance of giant HnRNA in the epidermal cells of Calliphora in the transition period from larva to pupa is ecdysone-dependent. In this specific stage of development, the initial increase of this RNA species occurs shortly after the appearance of a small peak of ecdysone. Another main peak of the hormone is a prerequisite for pupation. The removal of the ring gland, the source of ecdysone, prior to the appearance of the second peak of the hormone, was found to have no pronounced effect on HnRNA synthesis for at least 48 h after the operation. It is concluded that the larval pupal differentiation is a two-step process. Initially a gen activation occurs dependent on a low ecdysone titer. This is followed by a second final step of pupal cuticle synthesis and pupation, dependent on the presence of a high concentration of the hormone.
mesh_terms: D000818:Animals; D004175:Diptera / Q000378:metabolism*; D004440:Ecdysone / Q000502:physiology; D007700:Kinetics; D007814:Larva; D011679:Pupa; D012332:RNA, Heterogeneous Nuclear / Q000096:biosynthesis*; D012867:Skin / Q000378:metabolism
chemical_list: D012332:RNA, Heterogeneous Nuclear; D004440:Ecdysone