Decarboxylation and carboxylation of pyruvate in the living mice.
Mice received intravenously [1- or 2-14C]acetate, [1-, 2- or 3-14C] or [2-14C]pyruvate and were killed 1, 3, 5 or 15 min later. The radioactivity of CO2 or HCO3- of liver or carcass as well as the radioactivity of blood glucose were measured. The ratio of the radioactivity found in these compounds after [3-14C] or [2-14C-A1pyruvate injection suggests that in the fed aminals: 1. the decarboxylation of the pyruvate was more rapid than its carboxylation, 2. most of the neosynthesized glucose was derived from pyruvate molecules which had undergone a decarboxylation followed by a condensation to citrate, 3. 1/4 to 1/3 of the pyruvate was carboxylated and 2/3 to 3/4 was decarboxylated in animals receiving a diet poor in fats.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D003653:Decarboxylation; D004305:Dose-Response Relationship, Drug; D005943:Gluconeogenesis; D008099:Liver / Q000378:metabolism; D051379:Mice; D011773:Pyruvates / Q000378:metabolism*
D000085:Acetates; D001639:Bicarbonates; D011773:Pyruvates; D002245:Carbon Dioxide