[In vitro interaction between sex hormones and lysosomes isolated from rat liver and kidney].
With incubation in vitro of the liver and kidneys lysosomes of castrated rats together with 3H-testosterone and 3H-estradiol in the end concentrations of 2.38.10(-11) M (1 microCu) and 2.8.10(-10) M (2.49 microCu), respectively it was found that the lysosomes can bind labelled hormones. The treatment of lysosomes with membrane-toxic agents (90% aqueous acetone, phospholipase C and 0.1% triton X-100) results in the elimination of labelled steroids from them. In the interaction of 3H-testosterone and 3H-estradiol with lysosomes partake both proteins and lipids of the membranes.
D000818:Animals; D002369:Castration; D055598:Chemical Phenomena; D002621:Chemistry; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism*; D008297:Male; D010740:Phospholipases; D011092:Polyethylene Glycols; D051381:Rats; D012737:Sex Factors; D013739:Testosterone / Q000378:metabolism*
D011092:Polyethylene Glycols; D013739:Testosterone; D004958:Estradiol; D010740:Phospholipases
Vzaimodeĭstvie in vitro polovykh gormonov s lizosomami, vydelennymi iz pecheni i pochek krys.