[Effects of organic solvent vapors on the protochlorophyllic complex from etiolated leaves. Conditions for reversible and irreversible destruction].
The spectral properties and the ability of etyolated leaves pigments treated with organic solvent vapours (OS) for phototransformations were studied by measuring low temperature fluorescence spectra (-196 degrees C). Under the effects of OS the fluorescence at 655 nm was gradually decreased and that at 630--640 nm was increased. The effects of OS depended on the partial pressure of OS. The ability of the pigments for phototransformations was decreased with an increase in fluorescence at 630 nm. The emission maximum of fluorescence of the pigment formed in the light was shifted by 10--18 nm towards the shortwave region. Partial reversibility of the destroying effects of diethyl ester was found. A removal of the ester vapours resulted in a relative increase of fluorescence in the etyolated leaves at 640--645 nm and a decrease of the amount of "photo-inactive" pigment. The maximum of fluorescence of the pigment formed in the light was shifted towards the long-wave region (approximately 5 nm) as compared to the leaves irradiated in the presence of the ester. Partial functional reconstitution indicates that at least part of the pigment molecules are able to form a protochlorophyllide (protochlorophyll) -- protein complex, similar to the native one.
D002734:Chlorophyll / Q000031:analogs & derivatives; D005615:Freezing; D010788:Photosynthesis; D010940:Plant Proteins; D010944:Plants / Q000378:metabolism*; D011521:Protochlorophyllide / Q000378:metabolism; D012997:Solvents; D013050:Spectrometry, Fluorescence
D010940:Plant Proteins; D012997:Solvents; D002734:Chlorophyll; D011521:Protochlorophyllide
Deĭstvie parov organicheskikh rastvoriteleĭ na protokhlorofillovyĭ kompleks étiolirovannykh list'ev. Usloviia obratimogo in neobratimogo povrezhdeniia.