Solid-phase radioimmunoassay for the detection of antibodies to collagen.
A solid-phase radioimmunoassay has been developed using polystyrene tubes for the detection of class-specific antibodies to collagen. The optimal conditions for the adsorption of collagen onto the tubes, followed by incubation with the antisera and finally the radiolabelled antibody to the class-specific antibody are described. Studies carried out on antisera raised in rats and rabbits using this assay confirm its sensitivity and applicability.
D000818:Animals; D000906:Antibodies; D001665:Binding Sites; D001666:Binding Sites, Antibody; D002417:Cattle; D003094:Collagen / Q000276:immunology*; D006041:Goats; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats
D000906:Antibodies; D007106:Immune Sera; D007074:Immunoglobulin G; D003094:Collagen