The relative capacity of corneal, heart, kidney and skin cells to stimulate allogeneic lymphocytes.
In stimultaneous experiments, the ability of inbred Wistar-Furth rat corneal cells to stimulate inbred Fisher rat lymphocytes in mixed culture was compared with the stimulatory capacity of the same number of Wistar-Furth skin, kidney, heart and lymphocyte cells. The tissue cells were dissociated and after inhibition by mitomycin C cultured with an equal number of allogeneic spleen lymphocytes for 5 days. In all of these mixed cell cultures, the allogeneic lymphocytic response was mainly to the major Ag-B2 histocompatibility antigen. The stimulatory effect of corneal cells was found to be the same as that of heart, kidney and skin cells. The data indicate a lack of differences between the density of histocompatibility antigens on the surfaces of these cells.
D000818:Animals; D003315:Cornea / Q000276:immunology*; D005260:Female; D007668:Kidney / Q000276:immunology*; D008213:Lymphocyte Activation; D008623:Mercaptoethanol / Q000494:pharmacology; D009206:Myocardium / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D011920:Rats, Inbred WF; D012867:Skin / Q000276:immunology*
D008623:Mercaptoethanol