Preparation and stability of a liquid creatine kinase isoenzyme control from rabbit serum.
The MB isoenzyme of creatine kinase (CK) may be prepared in vitro from rabbit serum containing only the MM and BB isoenzymes, by means of a hybridization technique. The MM and BB dimers dissociate in 4 mol/L urea, which allows random recombination of M and B monomers. A liquid CK-isoenzyme control can be made from mixtures of rabbit sera obtained after hybridization and stabilized with glycerol and 25 mmol of 2-mercaptoethanol per liter. A liquid control stored at 4 degrees C showed good stability over a three-month period, declining to a mean residual activity of CK of approximately 90% after three weeks and a mean residual activity of MM, MB, and BB of 80--85% after six weeks. At 25 degrees C, CK activity of the liquid control declined to 75--80% after the fourth week. CK-BB at 25 degrees C was the least stable isoenzyme, declining to 75% after the third week and reaching 60% of activity after 12 weeks. CK-MB and CK-MM showed approximately 10--15% less stability at 25 degrees C than at 4 degrees C.
D000818:Animals; D003402:Creatine Kinase / Q000097:blood*; D004355:Drug Stability; D005990:Glycerol; D007527:Isoenzymes / Q000097:blood*; D008623:Mercaptoethanol; D011817:Rabbits; D013997:Time Factors
D007527:Isoenzymes; D008623:Mercaptoethanol; D003402:Creatine Kinase; D005990:Glycerol