title: Labile testosterone conjugate in human urine: further evidence.
abstract: The origin of unconjugated testosterone released in human urine on storage and by dilute sulphuric acid treatment has been studied further. Various extraction procedures of urine have been employed and a new methodology involving anion exchange followed by acid hydrolysis and radioimmunoassay of the released unconjugated testosterone. Results indicate that human urine contains a very labile conjugate of testosterone and 5 alpha-dihydrotestosterone with polar solubility properties and a negative charge.
mesh_terms: D002852:Chromatography, Ion Exchange; D013196:Dihydrotestosterone; D006801:Humans; D008297:Male; D012997:Solvents; D013739:Testosterone / Q000652:urine*
chemical_list: D012997:Solvents; D013196:Dihydrotestosterone; D013739:Testosterone