title: ATP-induced release of vasopressin from isolated bovine neurohypophyseal secretory granules. Dependency on chloride and effects of analogues of ATP.
abstract: Secretory granules isolated from bovine neurohypophyses released vasopressin in the presence of a buffered medium containing ATP, Mg2+ and KCl. Substitution of K+ in the medium with Na+ or choline did not affect the release. Substitution of Cl- with either sucrose, sulphate or acetate strongly reduced the release. Analogues of ATP, substituted at the beta-gamma anhydride bond with methylene or imido groups caused a smaller release which was not related to a very small breakdown of analogues that occurred. It is suggested that at least part of the ATP induced release is due to a physicochemical action.
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002712:Chlorides / Q000494:pharmacology*; D003470:Culture Media; D003594:Cytoplasmic Granules / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D010904:Pituitary Gland, Posterior / Q000166:cytology / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D014667:Vasopressins / Q000378:metabolism*
chemical_list: D002712:Chlorides; D003470:Culture Media; D014667:Vasopressins; D012965:Sodium Chloride; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D008274:Magnesium