title: Isolation of a branched-chain alpha-keto acid decarboxylase from rat liver.
abstract: An enzyme which catalyses oxidative decarboxylation of branched-chain alpha-keto acids was extracted from rat liver mitochondria with the aid of NaClO4. Purification yielded a product which appeared homogenous upon electrophoresis. Some kinetic data are reported; however, the enzyme is inactive with alpha-ketoisovalerate. The tenacity of binding to mitochondria, specificity, and other features, suggest that the decarboxylase may be a component of an enzyme complex named alpha-ketoisocaproate: alpha-keto-beta-methylvalerate dehydrogenase.
mesh_terms: D000597:Amino Acids, Branched-Chain; D000818:Animals; D002208:Caproates; D007651:Keto Acids; D007658:Ketone Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000302:isolation & purification* / Q000378:metabolism; D009997:Osmotic Pressure; D051381:Rats; D013379:Substrate Specificity; D014631:Valerates
chemical_list: D000597:Amino Acids, Branched-Chain; D002208:Caproates; D007651:Keto Acids; D009097:Multienzyme Complexes; D014631:Valerates; C016211:alpha-keto-beta-methylvaleric acid; C013082:alpha-ketoisocaproic acid; D007658:Ketone Oxidoreductases