Differentiation of aflatoxins from territrems.
Three methods were adopted for differentiation of aflatoxins B1 and B2 from territrems A and B. They were as follows. (i) Then-layer chromatography coupled with chemical confirmation. A significant decrease in the Rf value of trifluoroacetic acid-treated aflatoxin B1 developed in chloroform-acetone (85:15, vol/vol) was satisfactory in differentiating this toxin from the other three. (ii) High-pressure liquid chromatography monitored synchronously at two wavelengths, 365 and 335 nm. The ratio derived from this double-wavelengh detection could serve as an indicator of the presence of each toxin. (iii) Velasco's flurotoxin meter method, which is used for the determination of aflatoxins within the range of 0 to 50 ng/ml, was not significantly affected by territrems even when they were present in quantities at the microgram-per-milliliter level.
D000348:Aflatoxins / Q000032:analysis*; D001230:Aspergillus; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents; D009183:Mycotoxins / Q000032:analysis*; D013053:Spectrophotometry
D000348:Aflatoxins; D007202:Indicators and Reagents; D009183:Mycotoxins