Microinjection of ricin entrapped in unilamellar liposomes into a ricin-resistant mutant of baby hamster kidney cells.
Fluorescein-labelled Ricin was entrapped in unilamellar liposomes; 14 microgram of protein was entrapped by 1 mg of lipids. Liposomes added to cells in culture in low serum medium can deliver entrapped Ricin to a Ricin-resistant mutant of baby hamster kidney(BHK)cells. Ricin entrapped in unilamellar liposomes inhibits protein biosynthesis at a concentration of 1.75 microgram/ml in Ricin-resistant cells. Ricin dissolved in medium at 50 microgram/ml does not affect protein synthesis in these cells.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004351:Drug Resistance; D007668:Kidney; D007930:Leucine / Q000378:metabolism; D008081:Liposomes; D008845:Microinjections; D014176:Protein Biosynthesis; D012276:Ricin / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D008081:Liposomes; D012276:Ricin; D007930:Leucine; D013936:Thymidine