Light scattering turbidity changes as a measure of the kinetics of Ca2+ -promoted aggregation of chromaffin granule membrane ghosts.
Changes in turbidity seen when chromaffin granule membrane ghosts are aggregated by Ca2+ can be modelled as dimerization of hollow spheres using Rayleigh-Gans-Debye light-scattering theory. The experimental changes agree well with the calculations. Thus, if shape or refractive index changes produced by osmotic perturbation, ion uptake, etc. can be excluded, turbidity readings can be used to follow the progress of the aggregation reaction of storage vesicles and other small particles or macromolecules.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle; D002474:Cell-Free System; D002837:Chromaffin Granules / Q000648:ultrastructure*; D002838:Chromaffin System / Q000648:ultrastructure*; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology; D008027:Light; D009391:Nephelometry and Turbidimetry; D012542:Scattering, Radiation
D002118:Calcium