Simple modification of the cellulose acetate method for identifying adult hemoglobin (hemoglobin A) in dried specimens of newborn blood.
Definition of hemoglobin A in cellulose acetate electrophoretograms of eluate from air-dried specimens of newborns' blood was improved by adding glycerine to the Tris--ethylenediametetraacetic acid--borate buffer system. Examination of 1,672 randomly received samples showed no reduction in ability to detect hemoglobin S with the modified buffer. The method may be adopted as a useful adjunct to current technics for rapid electrophoretic screening of neonatal dried blood samples.
D001797:Blood Protein Electrophoresis / Q000379:methods*; D002021:Buffers; D004586:Electrophoresis / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods*; D005319:Fetal Hemoglobin / Q000032:analysis; D005990:Glycerol; D006441:Hemoglobin A / Q000032:analysis*; D006451:Hemoglobin, Sickle / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn
D002021:Buffers; D006451:Hemoglobin, Sickle; D006441:Hemoglobin A; D005319:Fetal Hemoglobin; D005990:Glycerol