title: Mononuclear cells contain human transfer factor as assayed locally on the skin of dogs.
abstract: We prepared transfer factor from mononuclear (M) cells and polymorphonuclear (PMN) cells by sieving cell lysates through a P-2 Biogel column. By assaying column fractions by their ability to induce a local delayed-type reaction to antigen in canine skin, we determined that mononuclear cells most consistently contained skin activity. The elution patterns from P-2 Biogel of PMN or M-enriched preparations were less complex and much different from that seen with whole leukocytes.
mesh_terms: D000042:Absorption; D000818:Animals; D002469:Cell Separation; D004285:Dogs; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D010586:Phagocytes / Q000276:immunology*; D012867:Skin / Q000276:immunology*; D013299:Streptodornase and Streptokinase / Q000276:immunology; D014165:Transfer Factor / Q000302:isolation & purification*
chemical_list: D014165:Transfer Factor; D013299:Streptodornase and Streptokinase