Mutations altering the catalytic activity of a plant-type ribulose biphosphate carboxylase/oxygenase in Alcaligenes eutrophus.
Use of a bacterial system has allowed the isolation of several mutationally altered species of ribulose-1,5-bisphosphate carboxylase/oxygenase having a wide range of catalytic activities. Biochemical and serological techniques were used to classify the mutant strains into three groups: (1) no detectable ribulose-1,5-bisphosphate carboxylase/oxygenase protein present (both subunits missing); (2) mutant strains having ribulose-1,5-bisphosphate carboxylase/oxygenases with lowered specific activities; and (3) mutant strains producing large quantities of catalytically inactive ribulose-1,5-bisphosphate carboxylase/oxygenase.
D000421:Alcaligenes / Q000201:enzymology* / Q000235:genetics; D002245:Carbon Dioxide / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D009154:Mutation; D010105:Oxygenases / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics* / Q000378:metabolism
D002245:Carbon Dioxide; D010105:Oxygenases; D002262:Carboxy-Lyases; D012273:Ribulose-Bisphosphate Carboxylase