title: An attempt for improving plating efficiency of human diploid fibroblasts.
abstract: An attempt was made to enhance the plating efficiency of normal human diploid skin fibroblasts by testing various culture media. Eagle's minimum essential medium (MEM), Dulbecco's MEM, William's medium E, DM-160, McCoy's 5a, RPMI-1640, Ham's F12, MCDB 102, Waymouth's MB 752/1, and L-15 synthetic media were examined. L-15 was the best among them. Plating efficiency with L-15 supplemented with 20% calf serum ranged from 20 to 60%. Plating efficiency decreased in the order of Eagle's MEM, Dulbecco's MEM, DM-160, and Ham's F12. Waymouth's MB 752/1, MCDB 102 McCoy's 5a, RPMI-1640, and William's medium E were not favorable for high plating efficiency. Although L-15 gave the best plating efficiency and Waymouth's MB 752/1 the worst, the Waymouth medium supported better cell proliferation than L-15, when cell proliferation was compared in mass culture with these two media. It was concluded that L-15 was better suited for culture of small number of cells, and especially for work involving colony formation.
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D004171:Diploidy; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans
chemical_list: D003470:Culture Media