[Accessibility of microsomal membrane proteins for protease K].
The effect of protease K on the microsomal membrane proteins was studied. It was shown that treatment of the microsomal membranes by low concentrations of protease did not remove more than 35% of total membrane proteins. Treatment by higher protease concentrations removed about 50% of the proteins. The residual protein is accessible to protease after treatment of the membranes by phospholipase A2. A simultaneous treatment of the microsomal membrane by protease and phospholipase removed up to 80% of the membrane proteins. In this way about 30% of microsomal membrane proteins are protected by the phospholipid against the action of protease K.
D000818:Animals; D010450:Endopeptidases / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008854:Microscopy, Electron; D008862:Microsomes, Liver / Q000648:ultrastructure; D010740:Phospholipases; D051381:Rats
D008565:Membrane Proteins; D010740:Phospholipases; D010450:Endopeptidases
Dostupnost' belkov mikrosomal'noĭ membrany dlia proteazy K.