title: Phomopsin A production by Phomopsis leptostromiformis in liquid media.
abstract: Phomopsis leptostromiformis WA1515 produced 75 to 150 mg of phomopsin A per liter in stationary cultures in a Czapek-Dox medium supplemented with 5 to 10 g of yeast extract per liter. pH and temperature optima were approximately 6.0 and 25 degrees C, respectively. A commercial tryptic digest of casein was a satisfactory alternative to the yeast extract, but poor growth and very little phomopsin were obtained when the yeast was replaced by vitamin-free Casamino Acids or a mixture of 18 amino acids. Approximately 95% of the phomopsin A produced was found in the cutlure liquid. No phomopsin was detected in shaken cultures. No phomopsin B was found in any culture. Methods are described for recovery and estimation of phomopsin A from culture liquids.
mesh_terms: D000818:Animals; D003470:Culture Media; D003904:Mitosporic Fungi / Q000254:growth & development / Q000378:metabolism*; D009183:Mycotoxins / Q000096:biosynthesis* / Q000633:toxicity; D010944:Plants / Q000382:microbiology*; D012756:Sheep
chemical_list: D003470:Culture Media; D009183:Mycotoxins