Purification and properties of 2'-nucleotidase from mammalian brain.
A nucleotidase specific for 2'-nucleotides was localized in both the soluble and the synaptosomal fractions of rat brain. The enzyme was partially purified from the soluble fraction of bovine brain. The s20,w was 4.9 S with an estimated molecular weight of about 70 000. The optimum pH was 8.0 and Km value for 2'-AMP was 5.5 . 10(-4) M. The substrate and inhibitor specificities of the enzyme were examined. The nucleotidase has an absolute requirement for Mg2+ but neither Fe3+ nor Ca2+ acted as replacement ions. In fact, Mn2+ and Ca2+ inhibited the Mg2+-dependent 2'-nucleotidase.
D000249:Adenosine Monophosphate; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002413:Cations, Divalent; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D009708:Nucleotidases / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity
D002413:Cations, Divalent; D000249:Adenosine Monophosphate; D009708:Nucleotidases