title: Staphylococcal protein A assay for detection of antibody directed at renal cancer cells.
abstract: Sera from 27 patients with renal cancer, 14 patients with transitional cell carcinoma of the bladder and 21 normal controls were tested against 2 renal cancer lines (CAKI-1 and CAKI-2) by the iodinated protein A assay. All sera tested against CAKI-2 were absorbed first with AB+ substance because CAKI-2 was found to have cell surface A antigen. Sera from 16 of 27 patients with renal cell carcinoma, 3 of 14 patients with transitional cell carcinoma of the bladder and 2 of 21 normal controls were reactive with CAKI-1. Sera from 11 of 27 patients with renal cell carcinoma, 2 of 14 patients with transitional cell carcinoma of the bladder and 3 of 21 normal controls were reactive with CAKI-2. There is a positive correlation between the number of counts obtained by the renal patients' sera tested against CAKI-1 and CAKI-2.
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000032:analysis*; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D002295:Carcinoma, Transitional Cell / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D003429:Cross Reactions; D006801:Humans; D007680:Kidney Neoplasms / Q000276:immunology*; D008297:Male; D011485:Protein Binding; D013205:Staphylococcal Protein A; D001749:Urinary Bladder Neoplasms / Q000276:immunology
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D013205:Staphylococcal Protein A