title: Biohydrogenation of unsaturated fatty acids. Presence of dithionite and an endogenous electron donor in Butyrivibrio fibrisolvens.
abstract: Two oxygen-consuming substances were isolated from cell-free extracts of the rumen anaerobe, Butyrivibrio fibrisolvens. The major fraction comprising 97% of the total activity was characterized as a three-component mixture of glucose, maltose, and dithionite. The minor activity fraction contained an electron donor for the reduction of cis-9,trans-11-octadecadienoate to trans-11-octadecenoate. After oxidation, the electron donor could be reduced by the dithionite, thereby accounting for the previously observed capacity of cell-free extracts of the bacterium to carry out the biohydrogenation of the conjugated dienoic fatty acid.
mesh_terms: D004227:Dithionite / Q000378:metabolism*; D004579:Electron Transport; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D006089:Gram-Negative Anaerobic Bacteria / Q000378:metabolism*; D007700:Kinetics; D010101:Oxygen Consumption; D013056:Spectrophotometry, Ultraviolet; D013447:Sulfites / Q000378:metabolism*
chemical_list: D005231:Fatty Acids, Unsaturated; D013447:Sulfites; D004227:Dithionite