Laser Raman spectroscopy of acid phosphatase from rat liver after neuraminidase treatment.
The conventional Raman technique was used to obtain spectra of acid phosphatase isoenzymes I and II from rat liver after neuraminidase enzyme treatment for 24 h. The low frequency bands below 300 cm-1 which were observed in native lyophilized isoenzymes are obtained from the sialic acid groups of these glycoproteins. These bands disappeared after neuraminidase treatment and the broad bands at 310, 260, 200 and 80 cm-1 are obtained from protein modes. The small differences between the Raman spectra of these isoenzymes disappeared after the neuraminidase treatment.
D000135:Acid Phosphatase; D000818:Animals; D007527:Isoenzymes; D007834:Lasers; D008099:Liver / Q000201:enzymology; D009439:Neuraminidase / Q000378:metabolism; D011487:Protein Conformation; D051381:Rats; D012794:Sialic Acids; D013059:Spectrum Analysis, Raman; D014867:Water
D007527:Isoenzymes; D012794:Sialic Acids; D014867:Water; D000135:Acid Phosphatase; D009439:Neuraminidase