Crystallographic studies of glutamate dehydrogenase. Preliminary crystal data.
Native and pyridoxal phosphate modified rat liver glutamate dehydrogenase crystals have been obtained and used for a preliminary x-ray crystallographic analysis. The space group is P6222 (P6422) having unit cell dimensions a = b = 101 A, c = 724 A and gamma = 120 A. The unit cell contains 36 subunits (six hexameric molecules) of molecular weight 56,000 and there is one half-molecule, i.e. three subunits, in the asymmetric unit. Packing considerations suggest that the glutamate dehydrogenase molecule has the point group symmetry 32 and that each subunit can be represented as a particle with approximate dimensions of 45 x 45 x 60 A.
D000818:Animals; D003460:Crystallization; D005969:Glutamate Dehydrogenase; D008099:Liver / Q000201:enzymology; D011487:Protein Conformation; D011732:Pyridoxal Phosphate; D051381:Rats; D014961:X-Ray Diffraction
D011732:Pyridoxal Phosphate; D005969:Glutamate Dehydrogenase