Sequential participation of glutathione and sulph-hydryl(s) in reductive dechlorination of 2,4-di-, and 2,4,5-trichloro phenacyl chlorides by soluble fraction (105,000 x g) of chicken liver homogenate.
The mode of reductive dechlorination of alpha-chloroacetophenones, 2,4-di-, and 2,4,5-trichloro phenacyl chlorides into respective acetophenone by soluble fraction (105,000 x g) from chicken liver homogenate has been investigated. The transformation involved the sequential participation of glutathione and a sulph-hydryl. The phenacyl chloride first reacted with glutathione to yield the phenacyl glutathione, which in turn, in the presence of a sulph-hydryl, was reduced enzymatically to produce the ketone, probably via a disulfide intermediate.
D000818:Animals; D001711:Biotransformation; D002645:Chickens; D003545:Cysteine / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D012995:Solubility; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013997:Time Factors
D006843:Hydrocarbons, Chlorinated; D013438:Sulfhydryl Compounds; D005978:Glutathione; D003545:Cysteine