title: UDP-glucosyltransferase activity of housefly microsomal fraction.
abstract: Housefly UDP-glucosyltransferase activity towards p-nitrophenol was demonstrated in a system in vitro. The activity is localized in the microsomal fraction, requires UDP-glucose, is slightly stimulated by Mg2+ and is activated optimally over a wide range of detergent concentration. Phenobarbital increases the enzyme(s) activity about 3-fold, with p-nitrophenol as substrate, which differs from the corresponding mammalian glucuronyltransferase.
mesh_terms: D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000378:metabolism*; D006793:Houseflies / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D014530:Uridine Diphosphate
chemical_list: D011092:Polyethylene Glycols; D014530:Uridine Diphosphate; D005964:Glucosyltransferases; D010634:Phenobarbital