Differentiation capacity of unincubated chick blastoderm in culture.
Unincubated (stage X) chick blastoderms cultured in plain Dulbecco's modified Eagle medium in the absence of serum, synthesize haemoglobin and melanin 2 . 5--3 . 0 and 6 . 0--7 . 0 days, respectively, after the beginning of culture. The capacity of unincubated blastoderm to form haemoglobin and melanin is a response to intrinsic genotypic instructions rather than to the active intervention of substances continuously supplied by the yolk. 5-Bromodeoxyridine, applied during the first and second days of unincubated blastoderm in culture, is incorporated into DNA and perturbs the formation of haemoglobin and melanin, perhaps, by modifying gene expression.
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D001756:Blastoderm / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002642:Chick Embryo; D004247:DNA / Q000096:biosynthesis; D006454:Hemoglobins / Q000096:biosynthesis; D066298:In Vitro Techniques; D008543:Melanins / Q000096:biosynthesis
D006454:Hemoglobins; D008543:Melanins; D004247:DNA; D001973:Bromodeoxyuridine