An improved method for the covalent attachment of glycolipids to solid supports and macromolecules.
A simplified method is presented for the oxidation of the olefinic bond of the sphingosine moiety of glycosphingolipids to a carboxyl group. Coupling of such "glycolipid acids" to glass beads, agarose gels, proteins, and polyacrylic hydrazide polymers is described. Solid supports and macromolecules that have been derivatized in this fashion are useful reagents for a variety of studies in cell biology and immunology.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005898:Glass; D006017:Glycolipids; D006028:Glycosphingolipids; D006801:Humans; D008722:Methods; D011108:Polymers; D011506:Proteins; D012685:Sepharose; D013110:Sphingosine
D006017:Glycolipids; D006028:Glycosphingolipids; D011108:Polymers; D011506:Proteins; D012685:Sepharose; D013110:Sphingosine