[Radioautographic study of a culture of fibroblasts after irradiation by a neodymium laser].
By means of radioautographic method, with 3H-thymidin application there were studied: mitotic activity, the number of DNA-synthesizing cells, growth fraction and population number of fibroblast culture irradiated with a single impulse of neodymium laser at energy density within 1 Dg/cm2--100 Dg/cm2. Under the influence of small doses of laser radiation (1--10 Dg/cm2) the number of dividing DNA-synthesizing cells increased, together with proliferative pool and the number of cells in the field of vision. At energy density of laser radiation equal to 50 Dg/cm2, and especially to 100 Dg/cm2 despite increased mitotic index and labelled cells index, registered during first hours of the effect, proliferative pool decreased and population growth rate lowered. These doses of laser radiation produced destruction of a rather large part of the cells in a monolayer.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004247:DNA / Q000096:biosynthesis / Q000528:radiation effects*; D005347:Fibroblasts / Q000528:radiation effects*; D007834:Lasers; D008938:Mitosis / Q000528:radiation effects*; D013997:Time Factors
D004247:DNA
Radioavtograficheskoe issledovanie kul'tury fibroblastov posle oblucheniia neodinovym lazerom.