title: Effect of temperature on alanine uptake by membrane vesicles isolated from a psychrophilic marine bacterium.
abstract: The effect of temperature on the membranes of Ant-300, a psychrophilic marine bacterium, was studied by measuring alanine uptake by isolated membrane vesicles. Uptake was observed from 0 to 35 degrees C. The maximum initial rate of uptake occurred at 25 degrees C although more alanine was ultimately taken up at temperatures from 10 to 20 degrees C. An ARRHENIUS plot of these data shows a single infection point at 7.8 degrees C. Within 10 min, over 50% of the alpha-aminoisobutyric acid taken up by whole cells at 5 degrees C was lost after a temperature shift to 25 degrees C. Vesicles preloaded with alanine at 5 degrees C did not become leaky when shifted to 25 degrees C. In addition, exposure of the vesicles to 25 degrees C for 30 min did not affect subsequent alanine uptake at 5 degrees C. The data obtained suggest that the loss of the uptake and permeability control functions of membranes from psychrophilic bacteria at elevated temperatures is not due to degeneration of the membrane itself, but rather to a control or regulatory mechanism associated with whole cells.
mesh_terms: D000409:Alanine / Q000378:metabolism*; D000864:Antarctic Regions; D000963:Antimetabolites / Q000494:pharmacology; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D013696:Temperature; D013997:Time Factors; D014733:Vibrio / Q000187:drug effects / Q000378:metabolism*; D014871:Water Microbiology
chemical_list: D000963:Antimetabolites; D000409:Alanine