title: [A new method of measuring serotonin in plasma and cerebrospinal fluid].
abstract: A new rapid method for the microdetermination of serotonin (5-hydroxytryptamine) in plasma and cerebrospinal fluid is described. A recovery coefficient of 98% was obtained in the present method, with the use of [3H]-hydroxytryptamine creatinine sulphate as an internal standard, compared with recovery coefficients of 31% and 66% for the two existing methods generally used for serotonin determination. This method involves a one-step sample deproteinization by perchloric acid, resulting in a high stability of serotonin.
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D005260:Female; D006801:Humans; D008297:Male; D008832:Microchemistry / Q000379:methods; D010215:Papio; D012701:Serotonin / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid
chemical_list: D012701:Serotonin
vernacular_title: 'n Nuwe metode vir die bepaling van serotonien in bloed en serebrospinale vloeistof.