Effects of cholinoceptor blocking drugs, adrenoceptor stimulants, and calcium antagonists on the transmurally stimulated guinea-pig urinary bladder in vitro and in vivo.
The effects of different drugs on the response to transmural electrical stimulation of the guinea-pig urinary bladder were studied in vitro and in vivo. In vitro, atropine (3.0 x 10(-8)-5.9 x 10(-4) M) did not influence the contractions. When used in high concentrations (greater than 5.2 x 10(-5) M), PR 197, another anticholinergic compound, reduced the responses by 25-40%, probably by a non-specific action. Noradrenaline (2.0 x 10(-6)--2.0 x 10(-4) M) and isoprenaline (2.0 x 10(-8)-2.0 x 10(-4) M) had concentration-related inhibitory effects that could be blocked by propranolol (5.2 x 10(-6) M). Adenosine (2.0 x 10(-2) M) inhibited the response by 27 +/- 3% (mean +/- S.E.M., n = 9). Theophylline (2.0 x 10(-5)-6.0 x 10(-4) M) had no consistent effects. The calcium antagonist nifedipone (1.2 x 10(-6)-1.7 x 10(-5) M) reduced the contractions by 25-50%; verapamil (2.2 x 10(-5)-4.4 x 10(-4) M) was little effective. In vivo, atropine (10 mg/kg) reduced the contractions by 55 +/- 5% (n = 10), whereas PR 197 (5 mg/kg) almost completely suppressed the responses. Noradrenaline (20-100 microgram/kg) and isoprenaline (20-300 microgram/kg) also caused a marked inhibition that could be blocked by propranolol (0.25-2.0 mg/kg). Theophylline (5 and 10 mg/kg) had a weak (10-20%) inhibitory effect. Adenosine (3.0 mg/kg) reduced the contractions by 47 +/- 4% (n = 14); in guinea-pigs pretreated with atropine (10 mg/kg), adenosine produced a further 10 to 20% decrease of the responses. Verapamil (0.5-2.0 mg/kg) had no consistent effect, whereas nifedipine (0.1-0.2 mg/kg) caused an inhibition of 20-50%. The results suggest that beta-adrenoceptor stimulants, and drugs with a combined anticholinergic and non-specific action, can effectively suppress the electrically evoked contractions in the guinea-pig urinary bladder.
D000241:Adenosine / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000037:antagonists & inhibitors*; D004558:Electric Stimulation; D005260:Female; D006168:Guinea Pigs; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D010276:Parasympatholytics / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011433:Propranolol / Q000008:administration & dosage / Q000494:pharmacology; D013566:Sympathomimetics / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D013806:Theophylline / Q000008:administration & dosage / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects*
D010276:Parasympatholytics; D013566:Sympathomimetics; D011433:Propranolol; D013806:Theophylline; D000241:Adenosine; D002118:Calcium