[Stabilizing effect of the antioxidants alpha-tocopherol and sodium selenite following phospholipase damage to mitochondrial membranes in a model of anoxia].
The kinetics of the free fatty acids accumulation in membranes of isolated mitochondria of the brain, heart and liver of rats, with their incubation in a medium containing no substrates and ATP, was studied. The intensity of the phospholipase damage, measured by the rate of free fatty acids accumulation, augments in the sequence of: the liver-heart-brain. The amassment of fatty acids in the mitochondrial membranes is shown to give place to their penetration into the inner space of the mitochondria. A relationship between the content of the intramitochondrial calcium and the concentration of free fatty acids in in the membranes, on the one hand, and the degree of passive swelling of the cardiac mitochondria, on the other, was revealed. It is suggested that the free fatty acids or their derivatives act as carriers of calcium to the mitochondrial membrane. A preliminary administration to rats of the vitamin E and sodium selenite protects the mitochondria of the heart against the phospholipase damage. These agents display a synergic action and are effective with administration of physiological doses. It is presumed that the protective action of these substances is due to the stabilization of biological membranes.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D000860:Hypoxia / Q000517:prevention & control*; D066298:In Vitro Techniques; D008928:Mitochondria / Q000032:analysis / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D008954:Models, Biological; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D051381:Rats; D012643:Selenium / Q000627:therapeutic use*; D014810:Vitamin E / Q000627:therapeutic use*
D005230:Fatty Acids, Nonesterified; D014810:Vitamin E; D010740:Phospholipases; D012643:Selenium; D002118:Calcium
Stabiliziruiushchee deĭstvie antioksidantov alpha-tokoferola i selenita natriia pri fosfolipaznom povrezhdenii membran mitokhondriĭ v usloviiakh model'noĭ anoksii.