DNA content and chromosome number of a heteroploid human tumour cell line.
In this paper investigations concerning the relation between variability of chromosome number and variability of DNA content within the cells of a tumour stemline are reported. A highly heteroploid human tumour cell line was used, which was derived from a chondrosarcoma. Flow cytometrical and scanning cytophotometrical measurements confirmed the heteroploid nature of the original cell line and of several subclones. Measurement of the DNA content per metaphase showed a linear relation between chromosome number and DNA content of heteroploid cells. This finding is discussed with regard to its implications for the mechanism of heteroploidy in tumour cells.
D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D002813:Chondrosarcoma; D002869:Chromosome Aberrations; D002877:Chromosomes, Human; D002999:Clone Cells; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D007399:Interphase; D007621:Karyotyping
D004273:DNA, Neoplasm