title: Protein synthesis in liver, muscle and gill of mullet (Mugil cephalus L.) in vivo.
abstract: 1. Protein synthesis in mullet was measured by use of pulse injections of 14Cleucine into the hepatic portal circulation and by 4-hr continuous infusion of 14Ctyrosine into the dorsal aorta. 2. Liver protein synthesis was 37 mg/day per g wet weight of tissue. Plasma proteins accounted for about 25% of total liver protein synthesis. Replacement rate for intrahepatic proteins was 20%/day. Average polypeptide chain synthesis time was 3 min. 3. Gill protein synthesis was comparable to that in liver with a replacement rate of 23%/day. 4. Muscle protein synthetic rate was 0.54%/day, comparable to rates in rabbit and sheep when temperature difference is taken into account. 5. Constant infusion is shown to be a feasible technique for measurement of protein metabolism of fish in vivo.
mesh_terms: D000818:Animals; D005399:Fishes / Q000378:metabolism*; D005880:Gills / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008722:Methods; D009132:Muscles / Q000378:metabolism; D014176:Protein Biosynthesis; D014443:Tyrosine / Q000378:metabolism
chemical_list: D014443:Tyrosine; D007930:Leucine