title: Enzyme-induced inactivation of transminases by acetylenic and vinyl analogues of 4-aminobutyrate.
abstract: The reactions of two analogues of 4-aminobutyrate, namely 4-aminohex-5-ynoate and 4-aminohex-5-enoate, with three transaminases were studied. Three pure enzymes were used, aminobutyrate transaminase (EC 2.6.1.19), ornithine transaminase (EC 2.6.1.13) and aspartate transaminase (EC 2.6.1.1), and the course of the reactions was studied by observing changes in the absorption spectrum of the bound coenzyme and by observing loss of activity. All of the enzymes were inactivated by either inhibitor, but amino-hexenoate showed a marked specificity for aminobutyrate transaminase. Aminohexynoate was most potent towards ornithine transaminase, and with this enzyme transamination of the inhibitor is an important factor in protecting the enzyme. Most of the reactions could be analysed as first order, with the observed rate constant showing a hyperbolic dependence on inhibitor concentration.
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000037:antagonists & inhibitors; D000614:Aminocaproates / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000037:antagonists & inhibitors; D055598:Chemical Phenomena; D002621:Chemistry; D007700:Kinetics; D009953:Ornithine-Oxo-Acid Transaminase / Q000037:antagonists & inhibitors; D013053:Spectrophotometry; D000637:Transaminases / Q000037:antagonists & inhibitors*; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000614:Aminocaproates; D005680:gamma-Aminobutyric Acid; D000637:Transaminases; D001219:Aspartate Aminotransferases; D009953:Ornithine-Oxo-Acid Transaminase; D000612:4-Aminobutyrate Transaminase