Isolation of brush border membranes in vesicular form from the intestinal spiral valve of the small dogfish (Scyliorhinus canicula).
A simple, rapid method for the preparation of purified brush border membranes in vesicular form from rabbit kidney proximal tubules has been applied with closely similar results to the intestinal spiral valve of the small dogfish (Scyliorhinus conicula). Since the dogfish belongs to one of the most ancient species of fish, it may be suggested that the method is generally applicable to all species later evolved which possess a brush border membrane at the mucosal surface of the cells of the intestine or kidney.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000648:ultrastructure*; D004284:Dogfish; D005947:Glucose / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000648:ultrastructure*; D007700:Kinetics; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure*; D012964:Sodium / Q000494:pharmacology
D012964:Sodium; D005947:Glucose