Disaturated and dipolyunsaturated phospholipids in the bovine retinal rod outer segment disk membrane.
Thin-layer chromatography was used to separate the major phospholipid headgroup classes of the rod outer segment disk membrane into subfractions which differ markedly in fatty acid composition. At least 18% of the rod outer segment phosphatidylcholine must contain two saturated fatty acids. Furthermore, two unsaturated fatty acids are found in at least 43% of the phosphatidylserine, 24% of the phosphatidylcholine, and 24% of the phosphatidylethanolamine. The unsaturated acids are predominantly polyunsaturated in all cases. A similar separation, but with less resolution, was achieved with silicic acid column chromatography. The temperature dependence of the polarization of the fluorescence of trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid) showed that the thermal behavior of aqueous dispersions of the phosphatidylcholine subfractions was consistent with their fatty acid compositions.
D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D010786:Photoreceptor Cells / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D005231:Fatty Acids, Unsaturated; D010743:Phospholipids