title: Macrophage-lymphocyte clusters in the immune response to soluble protein antigen in vitro. VII. Genetically restricted and nonrestricted physical interactions.
abstract: We have assessed the genetic restrictions on physical interactions between macrophages and  central lymphocytes and between central and peripheral lymphocytes in antigen-specific macrophage-lymphocyte clusters with respect to I-region differences of inbred strains 2 and 13 guinea pigs. When using lymphocytes from guinea pigs immunized with DNP-OVA or DNP-GL in CFA, the antigen-specific interaction between central lymphocyte and macrophage requires that both cells be derived from animals syngeneic at the I-region of the major histocompatibility complex. In studies using antigens, the responses to which is under the control of MHC-linked Ir genes, macrophages from the responder, but not from the nonresponder parental strain support cluster formation with responder x nonresponder F1(2 X 13) T cells. In contrast, the physical interactions between central and peripheral T lymphocytes are not restricted by the I-region of the MHC and the peripheral lymphocyte need not be from an animal immune to the antigen used to drive macrophage central lymphocyte interactions.
mesh_terms: D000818:Animals; D000941:Antigens / Q000235:genetics; D002478:Cells, Cultured; D004136:Dinitrobenzenes / Q000276:immunology*; D005971:Glutamates / Q000276:immunology; D006168:Guinea Pigs; D007106:Immune Sera / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology*; D008239:Lysine / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008285:Major Histocompatibility Complex; D009578:Nitrobenzenes / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology*; D012995:Solubility; D014373:Tuberculin / Q000276:immunology*
chemical_list: D000941:Antigens; D004136:Dinitrobenzenes; D005971:Glutamates; D007106:Immune Sera; D009578:Nitrobenzenes; D014373:Tuberculin; D010047:Ovalbumin; D008239:Lysine