The morphology of cytoplasts and karyoplasts produced by cytochalasin D.
The denucleation of L cells and human lymphocytes in suspension was carried out by incubation with cytochalasin D25 and 50 microgram/ml and with ultracentrifugation over a discontinuous Ficoll gradient. Ultrastructural examination of the separate layers confirmed the presence of the anucleate cytoplasm (cytoplasts) with intact membrane and the separated nuclei (karyoplasts).
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000648:ultrastructure*; D003572:Cytochalasins / Q000494:pharmacology*; D003593:Cytoplasm / Q000648:ultrastructure*; D006801:Humans; D007739:L Cells; D008214:Lymphocytes / Q000648:ultrastructure; D051379:Mice
D003572:Cytochalasins