[Possibility of purifying cholera toxin (choleragen) and its antibodies by an immunosorption method].
A study was made to establish the possibility of using an immune adsorbent based on polyacrylamide gel with the concentration of T = 10% and C = 25% for the purification of cholerogen and antibodies to it. For the isolation of cholera toxin the gamma globulin fraction of antitoxic serum was incorporated into the adsorbent; for obtaining antibodies an adsorbent with partially purified cholerogen was used. Desorption was made with 3 M sodium rhodanide solution. The yield of cholerogen was 41%, and the yield of antibodies was 4.5%. The non-specific sorption of the immune adsorbents was insignificant.
D000327:Adsorption; D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex; D002772:Cholera Toxin / Q000302:isolation & purification*; D005782:Gels; D007106:Immune Sera / Q000302:isolation & purification; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods; D007164:Immunosorbents; D011817:Rabbits; D014734:Vibrio cholerae / Q000276:immunology*
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D005782:Gels; D007106:Immune Sera; D007164:Immunosorbents; D002772:Cholera Toxin
Izuchenie vozmozhnosti ochistki kholernogo toksina (kholerogena) i antitel k nemu metodom immunosorbtsii.