Studies on in vivo priming of the TNP-reactive cytotoxic effector cell system. I. Comparison of the effects of intravenous inoculation with TNP-conjugated cells on the development of contact sensitivity and cell-mediated lympholysis.
A comparison was made of the effects of i.v. inoculation of trinitrophenyl-(TNP) conjugated syngeneic cells on the subsequent in vitro generation of TNP-reactive effector cell activity and on the in vivo development of TNP-contact sensitivity. The administration of syngeneic TNP-conjugated spleen cells before 2,4,6-trinitro-1-chlorobenzene (TNCB) painting abolished the capability of animals to develop TNP-contact sensitivity. In contrast, the same treatment resulted in an appreciable augmentation in the generation of TNP-reactive cytotoxic effector cell activity as measured by subsequent in vitro sensitization with TNP-conjugated cells. The possible mechanisms by which enhanced TNP-reactive cytotoxic effector cell activity was elicited under conditions identical to those that induced unresponsiveness for TNP-contact sensitivity are discussed.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D003877:Dermatitis, Contact / Q000276:immunology*; D005260:Female; D007156:Immunologic Memory; D007275:Injections, Intravenous; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009578:Nitrobenzenes / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D014301:Trinitrobenzenes / Q000008:administration & dosage / Q000276:immunology*
D009578:Nitrobenzenes; D014301:Trinitrobenzenes