title: Isolation of hydroxy-Y base from rat liver tRNAPhe.
abstract: A Y-base derivative was isolated from rat liver tRNAPhe and its structure was assigned to be alpha-(carboxyamino)-beta-hydroxy-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazol[1,2-a]purine-7-butyric acid dimethyl ester (hydroxy-Y), based on the results of mass spectrometry and chemical degradation. This modified base seems to be the major fluorescent component of rat liver tRNAPhe; the peroxy-Y base previously isolated from rat liver tRNAPhe and characterized by Nakanishi and his coworkers (1,2) was not present in our preparation.
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D008099:Liver / Q000032:analysis*; D013058:Mass Spectrometry; D010649:Phenylalanine; D011688:Purinones / Q000302:isolation & purification; D012343:RNA, Transfer; D051381:Rats
chemical_list: D011688:Purinones; D010649:Phenylalanine; C019430:hydroxy-Y base; D012343:RNA, Transfer