title: Routine identification and determination of 11 barbiturates in biological samples.
abstract: A very easy, reliable and specific gas-chromatographic method for identification and dosage of 11 barbiturates in plasma is presented. Methylene unit values determined on two types of column (one polar, one non-polar) allows very accurate identification with a minimum risk of error due to fluctuations in operating conditions. Sensitivity of the method is 0.5 microgram/ml plasma with a flame ionization detector. Derivatization procedure is complete, without any degradation phenomenon as tested by mass spectrometry. Such a method can be easily used as routine procedure for toxicological or pharmacological applications.
mesh_terms: D001463:Barbiturates / Q000032:analysis / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D008745:Methylation; D010424:Pentobarbital / Q000097:blood; D010634:Phenobarbital / Q000097:blood; D012631:Secobarbital / Q000097:blood
chemical_list: D001463:Barbiturates; D012631:Secobarbital; D010424:Pentobarbital; D010634:Phenobarbital