title: High performance liquid chromatography of unsaturated disaccharides produced from chondroitin sulfates by chondroitinase.
abstract: High performance liquid chromatography was performed by the ion pair method on unsaturated disaccharides produced from chondroitin sulfates by the action of chondroitinase. Completely separated peaks corresponding to deltaDi-0S, deltaDi-4S, and deltaDi-6S were obtained on a column of mu-bondapak-C18 with 0.035 M tetra-butylammonium phosphate (pH 7.54) as a mobile phase. There was a linear relationship between the ratio of the peak area and the amount of each standard tested.
mesh_terms: D002807:Chondroitin / Q000031:analogs & derivatives; D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004187:Disaccharides / Q000302:isolation & purification*
chemical_list: D004187:Disaccharides; D002807:Chondroitin; D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases