[Mechanism of activation of acetylcholinesterase in brain tissue homogenates by detergents].
The mechanisms of activation of acetylcholinesterase (AChE) in the homogenates of rat and frog forebrains and medullae oblongatae under effects of non--ionic detergents (Triton X-100 and Tw-en 80) were studied. Titration of the active centers of enzyme by the highly selective phosphoorganic inhibitor HD-42 showed that the inhibitor-induced activation is due to the appearance of previously masked active sites of the enzyme. Activation of AChE in the homogenates of rat brain medulla oblongata was caused by lysis of the structural elements of nervous tissue which were not destroyed by homogenization. Another mechanism of AChE activation by the detergents (frog brain homogenate) is the destruction of the vesicular membrane structures present in the homogenate with AChE centers located on the inner surface of the vesicles.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001001:Anura; D001921:Brain / Q000201:enzymology*; D004789:Enzyme Activation; D007700:Kinetics; D008526:Medulla Oblongata / Q000201:enzymology; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011136:Polysorbates / Q000494:pharmacology*; D051381:Rats
D011136:Polysorbates; D011092:Polyethylene Glycols; D000110:Acetylcholinesterase
Mekhanizm aktivatsii detergentami atsetilkhinestera3y V gomogenatakh golovnogo mozga.