Effects of chronic changes in hemoglobin-O2 affinity in rats.
We have assessed the characteristics of oxygen transport following chronic intraperitoneal administration of sodium cyanate (NaCNO, 90 mg/kg; P50 decreased), o-iodosodium benzoate (OISB, 300 mg/kg; P50 increased), or sodium chloride (NaCl; P50 unchanged) to rats at a rate of 5 times/wk for 16 wk. At the end of this period, the animals were exposed to a low inspired O2 concentration and were subjected to exercise stress. Hill's n50 at pH 6.90-7.60, hematocrit, and the saturation dependency of the Bohr effect (PCO2 constant) were altered by NaCNO only. Cyanate-treated rats gained less weight, probably due to a toxic side effect of the drug. Hypoxemia-induced lactatemia was greatest with a high P50 (OISB). Physical effort (swimming) was tolerated poorly at normal arterial PO2 when P50 was low (NaCNO). Although a left-shifted curve appears beneficial when FIO2 is reduced, reasons for the physiological advantage may be the result of other characteristics of the O2 dissociation curve, not P50 alone.
D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D003485:Cyanates / Q000494:pharmacology*; D006454:Hemoglobins / Q000378:metabolism*; D008433:Mathematics; D010100:Oxygen / Q000097:blood*; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000503:physiopathology; D013997:Time Factors
D001565:Benzoates; D003485:Cyanates; D006454:Hemoglobins; D012965:Sodium Chloride; D010100:Oxygen