[Interactions between macromolecular adjuvants and drugs. Part 16: Effects of human serum albumin and Mg2+ on oxytetracycline-polyvinylpyrrolidone interactions (author's transl)].
Equilibrium dialyses and fluorescent spectroscopic measurements demonstrated that human serum albumin (HSA) affects the oxytetracycline (OTC)--polyvinyl-pyrrolidone K 90 (PVP) interactions. The preferential binding of OTC with HSA, as compared to PVP, was evidenced by means of a three-compartment model. The binding values increased with the increase of the portion of PVP in the PVP-HSA mixture. Superadditive OTC bindings occurred at a ratio of 1:1 (by weight). The addition of Mg2+ to PVP-containing solutions, at a molar ratio of 1:1, also produced an increase of the interactions of this antibiotic with PVP, whereby maximal OTC-PVP bindings of 50--60% were reached.
D003956:Dialysis; D004347:Drug Interactions; D006801:Humans; D008274:Magnesium / Q000494:pharmacology*; D008954:Models, Biological; D010118:Oxytetracycline / Q000378:metabolism*; D011205:Povidone / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D012709:Serum Albumin; D011205:Povidone; D008274:Magnesium; D010118:Oxytetracycline
Wechselwirkungen zwischen makromolekularen Hilfsstoffen und Arzneistoffen. 16. Mitteilung: Beeinflussung der Oxytetracyclin-Polyvinylpyrrolidon-Wechselwirkungen durch Humanserumalbumin bzw. Mg2+.