Organ culture of adult mouse intestine. III. Behavior of the proteins, DNA content and brush border membrane enzymatic activities.
The proteins, the DNA content and the brush border membrane enzyme activities of adult mouse intestinal explants have been measured during a 24-hr organ culture. These activities were not modified in comparison with the controls at the beginning of the culture. The activity of the enzymes secreted in the medium during the 24-hr culture was equal to the activity present in the explants at the start of the culture. These results show that several metabolic functions of the intestine are fully preserved in organ culture.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D004247:DNA / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008871:Microvilli / Q000201:enzymology; D009924:Organ Culture Techniques; D011506:Proteins / Q000378:metabolism*
D011506:Proteins; D004247:DNA; D006867:Hydrolases; D000469:Alkaline Phosphatase; D006026:Glycoside Hydrolases