Proteolytic denaturation and methods of improving the stability of glucose isomerase preparations.
Evidence is provided in support of proteolytic denaturation of free and immobilized preparations of glucose isomerase from a Bacillus species. A number of methods to improve the stability with respect to proteolysis have been tested and their advantages as well as shortcomings are discussed. These methods include hollow-fiber treatment, gel permeation, thermal treatment, and addition of protease inhibitors. The half-life of the free and the cellulose acetate fiber-entrapped preparations of glucose isomerase can be significantly improved. For example, the hollow-fiber treatment can improve the half-life by an order of magnitude.
D000085:Acetates; D002238:Carbohydrate Epimerases / Q000302:isolation & purification / Q000378:metabolism*; D002482:Cellulose; D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005947:Glucose; D006358:Hot Temperature; D010447:Peptide Hydrolases / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology; D011489:Protein Denaturation / Q000187:drug effects
D000085:Acetates; D004800:Enzymes, Immobilized; D011480:Protease Inhibitors; D002482:Cellulose; D010447:Peptide Hydrolases; D002238:Carbohydrate Epimerases; D005947:Glucose