Lipid and salt effects on carbocyanine dye-induced photo-voltages in bilayer membranes.
Voltage transients induced by flash illumination of bilayer membranes with sorbed dye, 3,3' -dimethyl-2,2' -oxacarbocyanine chloride (diO-C1-3-CI), vary with membrane lipid composition and aqueous solution salt concentration. The voltage transients are probably induced by physical movements of sorbed dye molecules following photo-isomerization. Increased salt (NaCl and NaBr) concentrations in the aqueous solutions reduced the photo-voltage amplitudes by reducing the amount of dye sorbed to the membranes, and by decreasing the effective membrane thickness. The photo-voltage risetimes and falltimes varied systematically with salt concentration and membrane lipid composition, reflecting structural changes in the membrane's surface layer.
D002232:Carbocyanines; D002784:Cholesterol; D004396:Coloring Agents; D004563:Electrochemistry; D007700:Kinetics; D008027:Light; D008567:Membranes, Artificial; D008954:Models, Biological; D010084:Oxidation-Reduction; D010743:Phospholipids; D010777:Photochemistry; D011804:Quinolines; D012492:Salts
D002232:Carbocyanines; D004396:Coloring Agents; D008567:Membranes, Artificial; D010743:Phospholipids; D011804:Quinolines; D012492:Salts; D002784:Cholesterol