title: Preparation and stability of a liquid creatine kinase isoenzyme control from rabbit serum.
abstract: The MB isoenzyme of creatine kinase (CK) may be prepared in vitro from rabbit serum containing only the MM and BB isoenzymes, by means of a hybridization technique. The MM and BB dimers dissociate in 4 mol/L urea, which allows random recombination of M and B monomers. A liquid CK-isoenzyme control can be made from mixtures of rabbit sera obtained after hybridization and stabilized with glycerol and 25 mmol of 2-mercaptoethanol per liter. A liquid control stored at 4 degrees C showed good stability over a three-month period, declining to a mean residual activity of CK of approximately 90% after three weeks and a mean residual activity of MM, MB, and BB of 80--85% after six weeks. At 25 degrees C, CK activity of the liquid control declined to 75--80% after the fourth week. CK-BB at 25 degrees C was the least stable isoenzyme, declining to 75% after the third week and reaching 60% of activity after 12 weeks. CK-MB and CK-MM showed approximately 10--15% less stability at 25 degrees C than at 4 degrees C.
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000097:blood*; D004355:Drug Stability; D005990:Glycerol; D007527:Isoenzymes / Q000097:blood*; D008623:Mercaptoethanol; D011817:Rabbits; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D008623:Mercaptoethanol; D003402:Creatine Kinase; D005990:Glycerol