title: Activation of human large bowel adenylate cyclase by methylated prostaglandin E2 analogues.
abstract: The effects of methylated analogues of prostaglandin E2 upon the adenylate cyclase system in human colonic mucosa were tested. 16,16-dimethyl-prostaglandin E2 and 15-(S)-methyl-prostaglandin E2 increased the cyclase system in a dose-related manner. Maximal stimulation (about 250%) of enzyme activity occurred at a prostaglandin analogue concentration of 0.1 g/l. Diarrhea seen in patients treated with these orally effective prostaglandin analogues might in part be explained by activation of large bowel adenylate cyclase which is supposed to play a key role in the secretory process in this organ.
mesh_terms: D000262:Adenylyl Cyclases / Q000032:analysis*; D003106:Colon / Q000201:enzymology*; D003967:Diarrhea / Q000139:chemically induced; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D008745:Methylation; D011459:Prostaglandins E, Synthetic / Q000009:adverse effects / Q000494:pharmacology*; D013268:Stimulation, Chemical
chemical_list: D011459:Prostaglandins E, Synthetic; D000262:Adenylyl Cyclases