Concanavalin A and wheat germ agglutinin receptors in the ascitic fluid of rat hepatomas.
The presence of glycopeptide lectin receptors in the ascitic fluid of rats bearing Novikoff or AS-30D hepatoma was investigated. Macrosialoglycopeptides, resistant to pronase digestion, were partially purified from the ascitic fluid of hepatoma-bearing rats by gel filtration on Sephadex G-50. A macrosialoglycopeptide fraction, isolated from the ascitic fluid of rats bearing the Novikoff hepatoma, possessed potent concanavalin A (Con A) receptor activity. This fraction possessed higher Con A receptor activity than did the comparable macrosialoglycopeptide fraction from the ascitic fluid of rats bearing the AS-30D hepatoma; this obeservation is in agreement with the Con A-induced agglutination properties of these 2 hepatoma cell lines and with the Con A receptor activities of the glycopeptides released from the surface of the hepatoma cells by papain digestion. Rat blood serum contained a comparable macrosialoglycopeptide fraction, which possessed weak Con A receptor activity. The macrosialoglycopeptide fractions from the ascitic fluid of hepatoma-bearing rats possessed wheat germ agglutinin receptor activity. However, this activity was also present in normal rat serum. These results suggest that glycopeptides present on the surface of Novikoff hepatoma cells are shed into the ascitic fluid and may be distinguished from components in normal serum by their Con A receptor activity.
D000818:Animals; D001202:Ascitic Fluid / Q000032:analysis; D005260:Female; D006020:Glycopeptides / Q000302:isolation & purification; D037102:Lectins; D008114:Liver Neoplasms, Experimental / Q000032:analysis* / Q000097:blood; D051381:Rats; D011952:Receptors, Concanavalin A / Q000302:isolation & purification*; D011955:Receptors, Drug / Q000302:isolation & purification*; D012794:Sialic Acids / Q000097:blood / Q000302:isolation & purification
D006020:Glycopeptides; D037102:Lectins; D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D012794:Sialic Acids