Crossed immuno-electrodiffusion in the diagnosis of immunoglobulin fragment abnormalities.
The use of crossed immuno-electrodiffusion in the diagnosis of alpha-chain disease is described. The same basic technique is applicable in diagnosis of diseases in which immunoglobulin fragment abnormalities may occur. Crossed immuno-electrodiffusion is shown to be a superior method for demonstrating the abnormaility by providing more powerful resolution. Immunoselection (an adaptation of the classical method of immuno-electrophoresis) or rocket immuno-electrodiffusion both have limitations which are overcome by the use of crossed immuno-electrodiffusion. Mixture of antibodies is avoided by separating antibody-containing gels with interposition of intermediate gel layers free of antibody. Furthermore, precipitin rockets of some specific proteins can also be identified morphologically.
D004588:Electrophoresis, Cellulose Acetate; D006362:Heavy Chain Disease / Q000175:diagnosis* / Q000276:immunology*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007128:Immunoglobulin Fragments / Q000032:analysis*
D007128:Immunoglobulin Fragments