Identification of neurophysin-like proteins in the posterior pituitary gland of the chicken (Gallus domesticus).
Chicken posterior pituitary tissue was partially fractionated by extraction with 0.1 M HCl followed by gel exclusion chromatography. Two fractions, B and C, bound oxytocin. Two components of fraction B and at least 1 component of fraction C had properties characteristic of mammalian neurophysins: they appeared to be synthesized in the hypothalamus, were depleted upon osmotic stress, were rich in residues of cysteine and the 2 components of B bound to [8-lysinel]-vasopressin coupled to Sepharose.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002645:Chickens; D003545:Cysteine / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008236:Lypressin / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009481:Neurophysins / Q000302:isolation & purification / Q000378:metabolism*; D010121:Oxytocin / Q000378:metabolism; D010904:Pituitary Gland, Posterior; D011485:Protein Binding; D014868:Water Deprivation
D000596:Amino Acids; D009481:Neurophysins; D010121:Oxytocin; D008236:Lypressin; D008715:Methionine; D003545:Cysteine