title: Selective adherence of lymphocytes to myelinated areas of rat brain.
abstract: An in vitro system developed for studying lymphocyte binding to high endothelial venules (HEV) of lymph nodes was used to determine if there are similar binding sites in other organs of the rat. Thoracic duct lymphocytes (TDL) adhered selectively and uniformly to white matter when overlaid onto glutaraldehyde-fixed tissue sections of cerebellum and cerebrum. The pattern of TDL adherence to cerebellar sections showed that binding to nonmyelinated areas was negligible. Comparison of TDL-white matter to TDL-HEV binding demonstrated that the density of adherence to each site was quantitatively similar. In contrast, lymphocytes exhibited little tendency to bind to tissue sections of liver, spleen, heart, thymus, and salivary glands. TDL adherence to cerebellar white matter occurred rapidly, was cell dose dependent and optimal at 7 degrees C. White matter binding was also a property of spleen lymphocytes but the thymus was deficient in cells with this capability. The affinity of TDL and spleen lymphocytes for myelinated areas of the brain suggests the presence of myelin binding receptors on these cells.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion; D002531:Cerebellum / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D008198:Lymph Nodes / Q000098:blood supply; D008214:Lymphocytes / Q000276:immunology*; D009186:Myelin Sheath / Q000276:immunology*; D051381:Rats; D014699:Venules