Characterization of cholinergic control of vasopressin release by the organ-cultured rat hypothalamo-neurohypophyseal system.
Acetylcholine and nicotine stimulated vasopressin (VP) release from the organ-cultured rat hypothalamo-neurohypophyseal system (HNS). Nicotinic antagonists, hexamethonium, tetraethylammonium chloride, and trimethaphan blocked VP release in response to acetylcholine and nicotine. A muscarinic agonist, methacholine, was ineffective in eliciting VP release from HNS explants at a molar concentration equal to the maximally effective concentration of acetylcholine (10(-5) M). Atropine, a muscarinic antagonist, was an ineffective blocking agent for acetylcholine. These data indicate that the cholinergic receptor in the HNS explant is nicotinic rather than muscarinic in character.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D006584:Hexamethonium Compounds / Q000494:pharmacology; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008688:Methacholine Compounds / Q000494:pharmacology; D009538:Nicotine / Q000494:pharmacology*; D009924:Organ Culture Techniques; D051381:Rats; D011976:Receptors, Muscarinic / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000378:metabolism; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D014294:Trimethaphan / Q000494:pharmacology; D014667:Vasopressins / Q000378:metabolism*
D006584:Hexamethonium Compounds; D008688:Methacholine Compounds; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D013757:Tetraethylammonium Compounds; D014667:Vasopressins; D014294:Trimethaphan; D009538:Nicotine; D001285:Atropine; D000109:Acetylcholine