Enzymatic labeling of gamma-globulin and insulin with iodine-125.
The parameters of enzymatic labeling of proteins with iodine 125 were examined. The manner and sequence of reagent addition, the effects of reagent concentration, reaction time and total Na125I activity on the labeling yield were determined.
D007074:Immunoglobulin G; D007328:Insulin; D007457:Iodine Radioisotopes; D007467:Iodoproteins / Q000138:chemical synthesis* / Q000302:isolation & purification; D007784:Lactoperoxidase / Q000378:metabolism; D008832:Microchemistry / Q000379:methods; D013997:Time Factors
D007074:Immunoglobulin G; D007328:Insulin; D007457:Iodine Radioisotopes; D007467:Iodoproteins; D007784:Lactoperoxidase