Reiteration frequency of the gene coding for the small subunit of ribulose--1,5--bisphosphate carboxylase.
The messenger RNA coding for the precursor of the small subunit of ribulose--1,5--bisphosphate (Ru-P2) carboxylase has been partially purified from pea leaves. The mRNA has a size of 11.5S, is approximately 800 nucleotides long and, on cell-free translation, directs the synthesis of a single major polypeptide of 20,000 daltons. Antiserum for the small subunit of Ru-P2 carboxylase immunoprecipitated 52% of the 35S--methionine-labeled cell-free translation products. The RNA hybridizes with pea DNA with monophasic kinetics corresponding to the presence of one, or very few, gene copies per haploid genome.
D002262:Carboxy-Lyases / Q000235:genetics*; D007887:Fabaceae; D005796:Genes; D046911:Macromolecular Substances; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*
D046911:Macromolecular Substances; D012333:RNA, Messenger; D002262:Carboxy-Lyases; D012273:Ribulose-Bisphosphate Carboxylase