[Kinetics of macromolecular carbamoylation in normal and tumor cells after the administration of N-methyl-N-nitrosourea-14CO].
The pharmacokinetics of N-methyl-N-nitrosourea-14CO (14CO-MNU) was investigated in hepatoma 22a tumor-bearing and normal mice at various time after a single intravenous injection (80 mg/kg). The rapid clearance of 14CO-MNU from plasma of the mice was observed 5 hours following the administration of the drug. Peak levels of radioactivity in the hepatoma were noted 48 hours after the administration of 14CO-MNU, while the liver, kidney, spleen and intestinal epithelium showed an insignificant radioactivity at this time both in normal and tumor-bearing mice. The analysis of the radioactivity distribution among macromolecules (DNA, RNA, protein) and lipids indicated the highest label in lipids of all organs.
D000818:Animals; D002250:Carbon Radioisotopes; D004247:DNA / Q000378:metabolism; D004273:DNA, Neoplasm / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D007700:Kinetics; D050356:Lipid Metabolism; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism; D046911:Macromolecular Substances; D008297:Male; D008770:Methylnitrosourea / Q000627:therapeutic use*; D051379:Mice; D008809:Mice, Inbred C3H; D009363:Neoplasm Proteins / Q000378:metabolism; D009368:Neoplasm Transplantation; D009607:Nitrosourea Compounds / Q000627:therapeutic use*; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism
D002250:Carbon Radioisotopes; D004273:DNA, Neoplasm; D046911:Macromolecular Substances; D009363:Neoplasm Proteins; D009607:Nitrosourea Compounds; D011506:Proteins; D012334:RNA, Neoplasm; D012313:RNA; D008770:Methylnitrosourea; D004247:DNA
Kinetika karbamoilirovaniia makrokolekul normal'nykh i opukholevykh kletok posle vvedeniia N-metil-N-nitrozomocheviny-14CO.