Comparison of microchromatography and electrophoresis with elution for hemoglobin A2 (Hb A2) quantitation.
Microcolumns prepared in the authors' laboratory, two commercial microchromatography kits, and electrophoresis with elution were compared for Hb A2 quantitation. Day-to-day imprecision of microchromatographic methods was similar (CV 4.7--6.6%) and somewhat less than electrophoresis with elution (CV 8.0--9.1%). Both commercial kits showed variable imprecision in different lots; one lot of Kit B gave erratic results due to resin leakage. From 49 patient specimens, Kit A microcolumns and those of the authors identified the same 14 patients with an elevated percentage of Hb A2 and showed good correlation (P = 0.90), although Kit A showed constant bias toward higher values. Electrophoresis with elution resulted in a false-positive and a false-negative value, did not correlate well with microcolumns (P = 0.78 and 0.76), and showed proportional bias toward lower values for an elevated percentage of Hb A2. Commercial kits were convenient, relatively quick, and cost-effective. Frozen, stabilized hemolysates performed well for quality control.
D001797:Blood Protein Electrophoresis; D002848:Chromatography, DEAE-Cellulose; D003365:Costs and Cost Analysis; D006441:Hemoglobin A / Q000032:analysis*; D006443:Hemoglobin A2 / Q000032:analysis*; D013997:Time Factors
D006441:Hemoglobin A; D006443:Hemoglobin A2