Organ culture of adult mouse intestine. II. Mitotic activity, DNA synthesis and cellular migration after 24 and 48 hours of culture.
DNA synthesis, mitotic activity and cell migration have been measured in organ culture of adult mouse jejunum during the first 48 hr. Explants cultured in DMEM-HEPES-NCTC-135 medium present a sharp decrease of mitotic activity in the first hours of culture, but mitoses are restored to 80% of the controls between 24 and 48 hr. DNA synthesis follows the same pattern. Cell migration continues during culture.
D000818:Animals; D002465:Cell Movement; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004386:Duodenum / Q000166:cytology* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism; D007583:Jejunum / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008938:Mitosis; D009924:Organ Culture Techniques
D003470:Culture Media; D004247:DNA