The problem of interaction of shigella with epithelial cells.
A review and analysis of data on the investigation of factors concerning the initial events of interaction of Shigella with epithelial cells: attachment of penetration are presented. In the experiments with 3H-labeled bacteria it was shown that although common pili confer adhesive properties to bacteria but the penetration of Shigella into the cell is not increased. It was stated that LPS of Shigella O antigen plays an important role not only in interaction with cellular and humoral factors of the macroorganism defence but also in an initial stage of attachment of bacteria to the membrane of epithelial cells. However LPS of O antigen does not provide the penetration of the agent into the cell. In filtrates of virulent strains of Shigella flexneri, in contrast to those of smooth mutants and hybrids, lacking penetration ability, a biologically active factor (BAF) of protein or lipoprotein nature which reduced the LD50 of virulent and avirulent bacteria in infection of chicken embryos on chorioallantoic membrane, decreases ID50 and complicates the course of the infectious process in conjunctival infection of guinea pigs with virulent Shigella strain was revealed. S. flexneri transductant with a restored ability to induce kerato-conjunctivitis restored an ability to produce the BAF as well. Comparison of these data with the findings of other investigators was carried out. Perspectives of interpretation of penetration mechanism of Shigella into epithelial cells are discussed.
D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000502:physiology; D002448:Cell Adhesion; D002462:Cell Membrane; D004847:Epithelial Cells; D007637:Keratoconjunctivitis / Q000382:microbiology; D008074:Lipoproteins; D009154:Mutation; D012760:Shigella / Q000502:physiology*; D014774:Virulence
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008074:Lipoproteins