The development of mouse spinal cord in tissue culture. I. Cultures of whole mouse embryos and spinal-cord primordia.
Whole mouse embryos were grown in vitro from Theiler stage 12 (1 to 7 somites) to Theiler stages 15 and 16 (25 to 35 somites). This procedure gives experimental access to precisely staged embryos during the early period of neurogenesis. To follow the further development of neurons in vitro, fragments of spinal primordia were set up from these cultured embryos. In such cultures, the proliferation of precursor cells, the formation of postmitotic cells, and, finally, the cytodifferentiation of neurons were observed.
D000818:Animals; D002454:Cell Differentiation; D003470:Culture Media; D004622:Embryo, Mammalian; D051379:Mice; D009474:Neurons / Q000166:cytology; D009924:Organ Culture Techniques; D013116:Spinal Cord / Q000196:embryology*
D003470:Culture Media