[Change in the structural organization of the respiratory apparatus of Mycobacterium rubrum depending on the cultivation conditions].
Changes in the respiration apparatus of Mycobacterium rubrum were studied in the lag phase. The number of mitochondrial analogues was found to increase in parallel with the cellular growth, reaching a certain density of distribution per unit surface of the cells. The analogues of mitochondria were very labile. They rearranged easily, depending on the conditions of bacterial cultivation, but were always present in the cells as a discrete structure. The cellular growth and an increase of the mitochondrial analogues in them at 24 and 37 degrees C ceased by the 6-8th hr, regardless of the presence of glucose in the medium. Transition of the culture to the log phase at 24 degrees C was delayed to 16-18 hrs. After division, cells cultivated in media containing glucose were much larger than those grown in media without glucose, and the density of distribution in them of the mitochondrial analogues was higher. A decrease of temperature had no effect on the bacterial growth, but inhibited their division. The rate of oxygen uptake by cell suspensions at different periods of the lag phase correlated with the content of the mitochondrial analogues in them.
D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D009161:Mycobacterium / Q000254:growth & development / Q000648:ultrastructure*; D010101:Oxygen Consumption; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D005947:Glucose
Izmenenie strukturnoĭ organizatsii dykhatel'nogo apparata Mycobacterium rubrum v zavisimosti ot usloviĭ kul'tivirovaniia.