title: Opening of tight junctions in cerebral endothelium. II. Effect of pressure-pulse induced acute arterial hypertension.
abstract: Acute arterial hypertension was produced in male Wistar rats by pressure pulse through the right internal carotid artery. The pressure pulse was induced by infusion of physiological saline as a bolus, at a rate of 0.63 ml per second by syringe pump. Evans blue (Eb) was used to visualize the areas of blood-brain barrier opening. Intravenously injected horseradish peroxidase (HRP) was used to study the ultrastructural basis of permeability changes in cerebral endothelium. Eb outlined circumscribed areas of blood-brain barrier opening. HRP extravasation was found mainly around small arteries. The capillary network was affected to a much lesser extent. Electron microscopy showed that HRP crossed the endothelial cell layer by intercellular routes. Glutaraldehyde fixed brain samples permeated with colloidal lanthanum supported these observations.
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D004727:Endothelium / Q000648:ultrastructure; D006973:Hypertension / Q000473:pathology*; D007365:Intercellular Junctions / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats