Nature of RNAs synthesised in rat liver in response to hydrocortisone.
Experiments on hybridization of [32P]HnRNA from hydrocortisone-treated rat liver with rat-liver DNA, in competition with unlabelled liver HnRNA from hormone-deficient and treated rats, revealed marked differences in their nucleotide sequence homologies. It was found that the hormone-induced new species of HnRNAs were transcribed both on middle-repetitive and non-repetitive sequences of DNA. However similar differences in the sequence homologies were not detectable in respect of mRNA derived from livers of hydrocortisone-administered and adrenalectomised rats. The results imply that the new species of HnRNA synthesized following hormone treatment may be confined to the nucleus.
D000315:Adrenalectomy; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000096:biosynthesis*; D012332:RNA, Heterogeneous Nuclear / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats
D012332:RNA, Heterogeneous Nuclear; D012333:RNA, Messenger; D012313:RNA; D004247:DNA; D006854:Hydrocortisone