title: Purification of the chloroplast-membrane dicyclohexylcarbodi-imide-binding proteolipid by ion-exchange chromatography.
abstract: An efficient, mild and rapid procedure is reported for the separation of the dicyclohexyl-carbodi-imide-binding protein of chloroplast membranes from endogenous lipid components. By the use of ion-exchange chromatography the chloroplast proteolipid can be successfully separated from the major part of chlorophyll and other membrane lipids while being retained in a butan-1-ol milieu.
mesh_terms: D002234:Carbodiimides; D002736:Chloroplasts / Q000032:analysis*; D002848:Chromatography, DEAE-Cellulose; D004024:Dicyclohexylcarbodiimide; D004591:Electrophoresis, Polyacrylamide Gel; D007425:Intracellular Membranes / Q000032:analysis; D011485:Protein Binding; D011510:Proteolipids / Q000302:isolation & purification*
chemical_list: D002234:Carbodiimides; D011510:Proteolipids; D004024:Dicyclohexylcarbodiimide