[Effect of cycloheximide, 2,4-dinitrophenol and papaverine on ribosomal RNA synthesis in Ehrlich ascitic carcinoma cells].
The influence of 2,4-dinitrophenol (DNP), papaverine and cycloheximide on RNA synthesis in Ehrlich ascites tumour cells has been investigated. All above mentioned agents inhibit selectively synthesis of high-molecular rRNA precursor, when the cell population density is 3.10(7)--5.10(7) per 1 ml of suspension. When the density of cells decreases as far as 1.10(6) cells per 1 ml. the rRNA synthesis loses the sensitivity to all these agents. The effects of both cycloheximide on the protein synthesis and DNP on ATP level do not depend on the cell population density in suspension. It is suggested that either with a decrease of cell population density the protein synthesis and ATP level cease playing the role of a rate-limiting factor in the rRNA synthesis, or the influence of agents studied is realized by means of their interaction with other cell system.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology*; D003864:Depression, Chemical; D004140:Dinitrophenols / Q000494:pharmacology*; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010208:Papaverine / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis*
D004140:Dinitrophenols; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D000255:Adenosine Triphosphate; D003513:Cycloheximide; D010208:Papaverine
Vliianie tsiklogeksimida, 2,4-dinitrofenola i papaverina na sintez ribosomal'noĭ RNK v kletkakh astsitnoĭ kartsinomy Erlikha.