Preparation and properties of a standardized lipopolysaccharide from salmonella abortus equi (Novo-Pyrexal).
The paper describes the preparation of the lipopolysaccharide from Salmonella abortus equi as obtained by standardized methods. The include the extraction with pehnol/water followed by phenol/chloroform/petroleum ether extraction, ultra-centrifugation, electrodialysis and conversion to the uniform sodium salt form. Chemical composition and physico chemical properties are described. The preparation, which is free from contaminants, was tested for local Shwartzman reactivity, pyrogenicity, lethal toxicity, mitogenicity, reactivity towards complement and tumoricidal action.
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D008070:Lipopolysaccharides / Q000032:analysis / Q000302:isolation & purification* / Q000633:toxicity; D051379:Mice; D008815:Mice, Inbred Strains; D012475:Salmonella / Q000032:analysis*; D014461:Ultracentrifugation
D008070:Lipopolysaccharides