Improved method for morphine extraction from biological samples.
Methadone morphine, or naloxone extraction from brain homogenates, plasma, and urine is described. An aqueous sample was loaded on a surgical gauze support, which was washed with extracting solvents. Aqueous samples remained on the support, and nonpolar drugs partitioned into the lipophilic extracting solvent. The procedure recovered 80-100% of nanogram levels of methadone, morphine, or naloxone from biological samples. In addition, an approximate 10-fold timesaving capacity was demonstrated compared to standard liquid-liquid extraction techniques.
D000818:Animals; D001923:Brain Chemistry; D002855:Chromatography, Thin Layer; D008297:Male; D008691:Methadone / Q000032:analysis / Q000097:blood / Q000652:urine; D008722:Methods; D051379:Mice; D009020:Morphine / Q000032:analysis* / Q000097:blood / Q000652:urine; D009270:Naloxone / Q000032:analysis / Q000097:blood / Q000652:urine
D009270:Naloxone; D009020:Morphine; D008691:Methadone