title: Developmental changes in the pyruvate kinase isozymes of coho salmon.
abstract: Pyruvate kinase exists as two major isozymes in coho salmon. As in mammals and birds, one form is present in the early embryo and maintains a wide tissue distribution in adults. This salmonid type K shows anodal migration during electrophoresis at pH 7.5. The appearence of functional musculature in the developing embryos. In adult animals this second form is the only pyruvate kinase in muscle. Brain, kidney, liver and gill contain primarily the type K pyruvate kinase while heart contains both major forms along with three intermediate forms which presumably constitute a hybrid set. Since there is no additional isozyme restricted to gluconeogenic tissues, we conclude that a type L isozyme has not developed in these animals. The two major isozymes are immunologically distincy. Both forms are dubject to fructose 1,6-bisphosphate activation of phosphoenolpyruvate binding, but the magnitude of the effect is small. The affinities for phosphoenolpyruvate are similar, but salmon type K has hyperbolic saturation curves with this substrate and type M has sigmoidal saturation curves. While the immunological data indicates considerable divergence in structure, the kinetic parameters of the two forms have remained relatively similar.
mesh_terms: D000367:Age Factors; D000818:Animals; D003429:Cross Reactions; D007527:Isoenzymes / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D009132:Muscles / Q000201:enzymology; D011770:Pyruvate Kinase / Q000276:immunology / Q000378:metabolism*; D012474:Salmon / Q000254:growth & development* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D007527:Isoenzymes; D011770:Pyruvate Kinase