Investigation of a glycoprotein-protein mixture from urine of anemic patients by analytical isotachophoresis.
Nine glycoprotein-protein fractions prepared of erythropoietin-active crude urinary protein and separated by gel filtration on Sephadex G-50, ion exchange chromatography on Sephadex DEAE A-50, gel filtration on Sephacryl S-200 and Sepharose 6B were investigated by analytical isotachophoresis. 7 to 38 components could be separated of every chromatographic fraction investigated using chloride as leading and glycinate as terminating electrolyte. Improvement of resolution is possible by variation of buffer system conditions.
D000741:Anemia, Aplastic / Q000652:urine; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004586:Electrophoresis / Q000379:methods*; D004921:Erythropoietin; D006023:Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D011506:Proteins / Q000032:analysis*
D006023:Glycoproteins; D011506:Proteins; D004921:Erythropoietin