Altered RNA transport without derepression in a rat kidney tumor induced by dimethylnitrosamine.
RNA/DNA hybridization in the presence of competing RNA was used to study transcription and transport of RNA from the nucleus to the cytoplasm in a rat renal tumor induced by a single intraperitoneal injection of dimethylnitrosamine (DMN). Neither repression nor derepression of transcription of repetitive DNA can be detected in this tumor, but transcripts of all active families of such sequences are found in the tumor cytoplasm. This is the pattern of RNA change found in the chemically-induced hepatocellular carcinomas and contrasts with the pattern of virus-induced renal tumors in which both altered transport and derepression of host repetitive sequences have been demonstrated. The alteration of RNA transport is nearly complete in the kidney only two days after the above treatment, but there is no such alteration detectable in the kidney after nine days of feeding a liver carcinogen which is not carcinogenic to kidneys. This data supports the significance and specificity of the loss of ability to restrict certain RNA sequences to the nucleus early during malignant transformation.
D000818:Animals; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D004128:Dimethylnitrosamine; D005260:Female; D007668:Kidney / Q000378:metabolism; D007680:Kidney Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism*; D051381:Rats; D014158:Transcription, Genetic
D012334:RNA, Neoplasm; D012313:RNA; D004128:Dimethylnitrosamine