[Antiaccelerative and antiarrhythmic effect of veratramin on myocardial cells in culture].
It is shown that veratramin (2.5 X 10(-5)M) inhibits, partially or completely, the accelerative effect of adrenalin (5.5 X 10(-7) M), isoproterenol (10(-7) M), prostaglandin E2 (5 X 10(-5) M), and glucagon (10(-5) M) in experiments on cultures of newborn rat cardiac cells and the effect of histamine on 3--4-week embryo cultures. Veratramin (1.25--25 X 10(-5) M) arrests digitoxin-induced (1.25 X10(-5) M--5 X 10(-5) M) fibrillation of individual cells and of cell groups. Veratramin alone and in combination with adrenalin does not change the inotropic parameters of individual cells. Bearing in mind that veratramin (2.5 X 10(-5) M) inhibits the accelerative effect of substances which activate and those which do not activate adenylatecyclse, as well as the fact that it has no effect on the content of cAMP in cultures and does not correct the increase in the cAMP content induced by adrenalin (5.5 X 10(-7) M), the authors conclude that the chronotropic effect of veratramin is not associated with the cell receptor mechanisms but is due to its effect on the transport of ions through the cell membrane, which is a responsible process in the generation of impulses.
D000818:Animals; D002478:Cells, Cultured; D003864:Depression, Chemical; D004074:Digitoxin / Q000494:pharmacology; D004336:Drug Antagonism; D004622:Embryo, Mammalian; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D005934:Glucagon / Q000494:pharmacology; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects*; D006632:Histamine / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D009206:Myocardium; D011247:Pregnancy; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology; D051381:Rats; D011941:Receptors, Adrenergic / Q000187:drug effects; D013268:Stimulation, Chemical; D014704:Veratrum Alkaloids / Q000494:pharmacology*
D011459:Prostaglandins E, Synthetic; D011941:Receptors, Adrenergic; D014704:Veratrum Alkaloids; D006632:Histamine; D005934:Glucagon; D004074:Digitoxin; D007545:Isoproterenol; D004837:Epinephrine
Protivouskoriaiushchee i antiaritmicheskoe deĭstvie veratramina na serdechnye kletki v kul'ture.