Liver injury by the false morel poison gyromitrin.
After oral application of the mushroom poison gyromitrin a time and dose dependent decrease of cytochrome P-450 was found in rat liver microsomes. The maximal decrease to about 50-60% of the control (after 200 mg/kg, 80% of LD50) was observed 8-12h after application, a normalization after 48 h. The inhibition of cytochrome P-450 mediated metabolism of aminopyrine and p-nitroanisole corresponds to the decrease of cytochrome P-450. The specific activity of cytochrome P-450 remains unchanged while that of cytochrome P-448 is decreased as shown by means of the metabolism of ethoxycoumarin or ethoxyresorufin. Comparable results were obtained after application of N-methyl-N-formylhydrazine (MFH) which is formed from gyromitrin rapidly by hydrolysis. An attack on the endoplasmatic membrane with a stimulation of lipid peroxidation is discussed.
D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006591:Hexobarbital / Q000494:pharmacology; D006835:Hydrazones / Q000378:metabolism / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009002:Monomethylhydrazine / Q000031:analogs & derivatives / Q000378:metabolism; D009145:Mushroom Poisoning / Q000378:metabolism*; D009183:Mycotoxins / Q000378:metabolism*; D009575:Nitroanisole O-Demethylase / Q000378:metabolism; D051381:Rats; D012890:Sleep
D006835:Hydrazones; D009183:Mycotoxins; D000632:Aminopyrine; C016577:N-methyl-N-formylhydrazine; D003577:Cytochrome P-450 Enzyme System; D006591:Hexobarbital; D006899:Mixed Function Oxygenases; D009575:Nitroanisole O-Demethylase; D000079:Acetaldehyde; C005666:gyromitrin; D009002:Monomethylhydrazine