Detection of Fc receptor-bearing lymphocytes by using IgG-coated latex particles.
Latex particles coated with human IgG can be used to detect lymphocytes capable of binding to the Fc portion of IgG. Optimal conditions are described. The method is as sensitive as techniques using antibody-coated erythrocytes, is easy to perform, and has good reproducibility. A small proportion of T cells and the majority of B cells can be shown to carry receptors for Fc (IgG).
D001666:Binding Sites, Antibody; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D007074:Immunoglobulin G; D007840:Latex; D008214:Lymphocytes / Q000276:immunology*; D008722:Methods; D008863:Microspheres
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007840:Latex