Gas-liquid chromatographic determination of monosodium glutamate in soups and soup bases.
A method is given for determining monosodium glutamate (MSG) in soups and soup bases by gas-liquid chromatography (GLC) of the trimethylsilyl ether derivative of glutamic acid. This method compared favorably with existing methods including GLC of the trifluoroacetate (TFA)/butyl ester derivative, and analysis on an amino acid analyzer. In addition, some samples were analyzed by GLC/mass spectrometry. The MSG content of various products ranged from approximately 0.2% in some condensed soups to 13.1% on bouillon cubes. The method, which can detect as little as 0.05%, requires only a 10 min single step derivatization at room temperature and is preferred to the TFA/butyl ester technique.
D002849:Chromatography, Gas; D005504:Food Analysis; D005971:Glutamates / Q000032:analysis*; D008722:Methods; D012970:Sodium Glutamate / Q000032:analysis*
D005971:Glutamates; D012970:Sodium Glutamate