Evaluation of the BMC glucose oxidase/peroxidase-4-aminophenazone-phenol procedure for glucose as adapted to the Technicon SMAC.
We evaluated the analytical performance of Trinder's glucose oxidase (EC 1.1.3.4)/peroxidase (EC 1.11.1.7) 4-aminophenazone-phenol method for the quantification of serum glucose as adapted to the Technicon SMAC. Our results correlated well with those by the routine SMAC glucose oxidase/peroxidase 3-methyl-2-benzothiazolinone hydrazone-N,N-dimethylaniline method (y = 1.02x - 49.4; r = 0.99) and the glucose oxidase oxygen-rate method (y = 0.99x + 14; r = 0.99) with the Beckman Glucose Analyzer. Sample-to-sample interaction was less than 1%. Ascorbic acid or uric acid in concentrations as high as 200 mg/L were without demonstrable effect on results for glucose. Intra- and inter-assay precisions (CV) were 1.6 and 2.3%, respectively. The upper limit of linearity was about 5 g/L. Adaptation of the Trinder method for glucose to the SMAC is simple and provides an analytically acceptable and economical alternative to the methods ordinarily used with the SMAC.
D000293:Adolescent; D000328:Adult; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D005215:Fasting; D005260:Female; D005949:Glucose Oxidase; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008875:Middle Aged; D010544:Peroxidases; D012016:Reference Values
D001786:Blood Glucose; D007202:Indicators and Reagents; D005949:Glucose Oxidase; D010544:Peroxidases