In vivo degradation of rat globin messenger RNA during maturation of reticulocytes.
The degradation of globin mRNA in rat reticulocytes maturing in the peripheral blood was investigated. Poly(A) and non poly(A) portions of mRNA molecules were determined quantitatively by hybridization with radioactive poly(U) and complementary DNA, respectively. During the degradation of mRNA in vivo, it was shown that (1) globin mRNA and the bulk of RNA decrease in parallel, (2) the average chain length of poly(A) segments in the mRNA does not change, (3) the percentage of poly(A) (-) globin mRNA in total globin mRNA does not change, and (4) fragments of large molecular weight do not accumulate. Possible mechanisms of degradation of globin mRNA in the reticulocytes are discussed on the basis of these observations.
D000818:Animals; D004905:Erythrocyte Aging; D005914:Globins / Q000096:biosynthesis*; D007700:Kinetics; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000097:blood; D012333:RNA, Messenger / Q000097:blood*; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism*
D012333:RNA, Messenger; D011061:Poly A; D005914:Globins