Pyridoxal 5'-phosphate binding site of pig heart alanine aminotransferase.
After borohydride reduction, carboxymethylation, and tryptic digestion of the holoenzyme of pig heart alanine aminotransferase, a single icosapeptide containing the N6-(phosphopyridoxyl)lysine residue was isolated by a combination of gel filtration and ion-exchange chromatogrpahy. Its primary structure was determined as Gln-Glu-Leu-Ala-Ser-Phe-His-Ser-Val-Ser-Lsy(Pxy)-Gly-Phe-Met-Gly-Glu-Cys-Gly-Phe-Arg.
D000410:Alanine Transaminase / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010455:Peptides / Q000032:analysis; D011732:Pyridoxal Phosphate / Q000378:metabolism*; D013552:Swine; D014357:Trypsin
D010455:Peptides; D011732:Pyridoxal Phosphate; D000410:Alanine Transaminase; D014357:Trypsin