title: Production of nerveless Hydra attenuata by hydroxyurea treatments.
abstract: Hydroxyurea was used to produce hydra with varying nerve cell densities including a new type of nerveless animal. Hydra attenuata were treated with 10(-2) M hydroxyurea. By 23 days after treatment, 2 populations of animals are in culture. Both have a decrease in nerve cells. The first is a normal-coloured feeding animal (HU-R) and is recovering while the second is a pale non-feeding animal (HU-P). HU-P animals resemble nerveless animals in their lack of behavioural responses but they contain about 2% nerve cells. Upon hand feeding, some HU-P animals will recover but most will produce nerveless buds. Neverless hydra produced by hydroxyurea resemble nerveless animals produced by other techniques, in their behavioural, morphological and developmental properties. Normal animals, as cultured and in regeneration experiments, show a compact tentacle number distribution pattern with a small variance. Nerveless animals show broad tentacle distribution patterns with increased means and variances. It is suggested that a normal tentacle number regulatory mechanism is lacking or diminished in nerveless animals. This defect is correlated with hypostomal circumference and with nerve cells.
mesh_terms: D000818:Animals; D002452:Cell Count; D006829:Hydra / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D006918:Hydroxyurea / Q000494:pharmacology*; D008854:Microscopy, Electron; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D012038:Regeneration
chemical_list: D006918:Hydroxyurea