[Affinity sorbents for obtaining plasminogen].
Using immobilization of l-lysine on cellulose and CM-Cellulose activated with bromo-cyanogen and dicyclohexyl carboxyimide, respectively, the affined sorbents are obtained and possibility of their application for obtaining the high-purified plasminogen is studied. According to the yield and activity of plasminogen, l-lysine-cellulose and l-lysine-CM-cellulose are not inferior to the known l-lysine-sepharose. With electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulphate the plasminogen preparations obtained by the method of affined chromatography are homogeneous and their molecular mass is 87 000 Daltons.
D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D008239:Lysine; D008970:Molecular Weight; D010958:Plasminogen / Q000302:isolation & purification* / Q000378:metabolism
D010958:Plasminogen; D008239:Lysine
Affinnye sorbenty dlia polucheniia plazminogena.