Polyadenylate-protein complexes in resting and growing 3T3 cells.
The properties of the ribonuclease resistant cytoplasmic ribonucleoprotein particles were studied in contact-inhibited and serum induced proliferating 3T3 cells. The RNP particles were fractionated by oligo (dT)-cellulose chromatography and banded in CsSO4 gradients. The main RNP fraction, eluted with 25% formamide, contained the major ribonuclease resistant RNA sequences in both resting and growing cells. The protein component of this fraction had a molecular weight of about 72,000 in contact-inhibited cells and 81,000 in serum induced cells.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003260:Contact Inhibition; D051379:Mice; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis*; D011061:Poly A / Q000032:analysis*; D012260:Ribonucleases; D012261:Ribonucleoproteins / Q000032:analysis*
D009698:Nucleoproteins; D012261:Ribonucleoproteins; D011061:Poly A; D012260:Ribonucleases