Selective measurement of triacylglycerol lipase activities in pig post-heparin plasma.
Immunochemical methods for the selective measurement of pig post-heparin plasma lipoprotein lipase and hepatic lipase are described and validated. A simple two step purification method for porcine hepatic lipase from hepatic perfusate based on affinity chromatography and gel filtration is reported. The activity of the post-heparin plasma lipoprotein lipase and hepatic lipase in swine is reported. It is demonstrated that fasting decreases the activity of post-heparin plasma lipoprotein lipase activity more than two-fold while it does not affect the hepatic lipase activity significantly.
D000818:Animals; D002846:Chromatography, Affinity; D005215:Fasting; D006493:Heparin / Q000494:pharmacology; D008049:Lipase / Q000097:blood* / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D013552:Swine; D014280:Triglycerides
D014280:Triglycerides; D006493:Heparin; D008049:Lipase; D008071:Lipoprotein Lipase