Effects of MK-196 on urate and electrolyte excretion in the rat.
Clearance and micropuncture techniques were used to evaluate the effects of MK-196 on uric acid and electrolyte excretion by the rat kidney. The urinary excretion of sodium, uric acid, calcium and magnesium increased significantly following MK-196 administration. The major site of action with respect to sodium reabsorption was in the ascending limb of Henle's loop as revealed by depression of both free-water clearance and reabsorption. By contrast, microinjection studies with [2-14C]-urate revealed the major site of altered urate absorption to be in the proximal convoluted tubule, a site where sodium and water reabsorption was unchanged.
D000818:Animals; D002118:Calcium / Q000652:urine; D004232:Diuretics / Q000494:pharmacology*; D004976:Ethacrynic Acid / Q000031:analogs & derivatives*; D007677:Kidney Function Tests; D007684:Kidney Tubules / Q000187:drug effects*; D007686:Kidney Tubules, Distal / Q000187:drug effects; D007687:Kidney Tubules, Proximal / Q000187:drug effects; D008274:Magnesium / Q000652:urine; D008297:Male; D009318:Natriuresis / Q000187:drug effects; D051381:Rats; D014528:Uricosuric Agents / Q000494:pharmacology*
D004232:Diuretics; D014528:Uricosuric Agents; D008274:Magnesium; D004976:Ethacrynic Acid; D002118:Calcium