Influence of fluoride on the uptake of 51Cr-chromate by human erythrocytes.
Uptake of 51Cr-chromate by freshly collected human erythrocytes was inhibited by 90 mM sodium fluoride only in cells preincubated with fluoride. In vitro storage also increased the sensitivity of the cells: 90 mM NaF clearly inhibited the uptake of chromate in cells stored in vitro for 5 weeks. Gel chromatography revealed two forms of 51Cr within the cells, one of small molecular weight, which thin layer chromatography indicated was unchanged chromate; the other form was apparently bound to macromolecules such as hemoglobin.
D001692:Biological Transport / Q000187:drug effects; D002840:Chromates / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005459:Fluorides / Q000494:pharmacology*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007700:Kinetics
D002840:Chromates; D006454:Hemoglobins; D005459:Fluorides