title: The control of the synthesis of isocitrate lyase in a thermophilic bacillus.
abstract: From a strain of Bacillus stearothermophilus, devoid of active pyruvate carboxylase, a mutant (NG-15) was selected that grew on acetate in the presence of glucose. This mutant differed from its parent organism in possessing high activities of isocitrate lyase when grown on all carbon sources tested except nutrient broth, in possessing unusually low activities of NADP+-dependent isocitrate dehydrogenase and in containing increased amounts of isocitrate. Revertants of mutant NG-15 which regained the ability to synthesize active pyruvate carboxylase also synthesized isocitrate lyase and isocitrate dehydrogenase to the same extent as the wild-type strain. These results suggest that the regulatory mechanism for the synthesis of isocitrate lyase in the thermophile may be different from that in mesophilic bacilli.
mesh_terms: D000085:Acetates / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism; D007522:Isocitrate Lyase / Q000096:biosynthesis*; D009154:Mutation; D007652:Oxo-Acid-Lyases / Q000096:biosynthesis*
chemical_list: D000085:Acetates; D006038:Glyoxylates; D007652:Oxo-Acid-Lyases; D007522:Isocitrate Lyase; D005947:Glucose