Derivative electrophoretograms and their applications.
A differentiator was constructed to produce the first and second derivative curves of routine cellulose acetate membrane electrophoretograms of human serum protein. The derivative curves could reveal the presence of multicomponent in each globulin fraction. The "M" components could be detected easily and semiquantitatively, when the cellulose acetate membrane electrophoretic pattern was evaluated by the derivative curves. Utilization of the derivative curves could be a useful adjunct to the detection of an "M" component since the presence of a narrow band produces a sharper peak on the second derivative curve and more indentation on the first derivative curve.
D001797:Blood Protein Electrophoresis / Q000379:methods*; D003201:Computers; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D006801:Humans