[Erythroid precursors studied by mouse bone marrow cultivation in a plasma clot].
Two types of erythroid precursors were found by cultivation of the mouse bone marrow in the plasma clot with mouse serum and without adding exogenic erythropoietin to the culture medium. The first precursor had properties similar to the erythroid colony-forming unit (CFUe) previously described while the second resembles in its properties the erythroid burst-forming unit (BFUe). Optimal concentration of mouse serum in the culture medium was 10-15%. Clone nature of the colonies and bursts described is confirmed by linear dependence of their number on the cell concentration in the culture.
D000818:Animals; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004912:Erythrocytes / Q000166:cytology*; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013997:Time Factors
D003470:Culture Media; D004921:Erythropoietin
Issledovanie éritroidnykh predshestvennikov metodom kul'tivirovaniia kostnogo mozga mysheĭ v plazmennom sgutke.