Density gradient centrifugation of cells separated from multicellular tumor spheroids.
Cells from a murine fibrosarcome (FSa) have been grown in vitro as multicell tumor spheroids (MTS). The growth rate of these MTS was determined. Following selected periods of growth, MTS were made into a single cell suspension and separated on linear density gradients of Renografin. While only 1 population of cells were separated from small spheriods (400 mum diameter), at least 3 subpopulations of tumor cells were separated and isolated from large spheroids (800 mum in diameter).
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D002500:Centrifugation, Isopycnic; D005354:Fibrosarcoma / Q000473:pathology; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology*