A sensitive fluorescent assay for N-acetyltransferase activity in human lymphocytes from newborns and adults.
We have developed a simplified assay for the enzyme N-acetyltransferase, based upon the loss of fluorescence after acetylation of the substrate p-aminobenzoic acid. This method is sufficiently sensitive to permit the quantitation of N-acetyltransferase activity in 10(5) human lymphocytes. Using this method, we have compared the level of N-acetyltransferase activity in lymphocytes from adult peripheral blood and from cord blood samples.
D000123:Acetyltransferases / Q000097:blood*; D000328:Adult; D000367:Age Factors; D005312:Fetal Blood / Q000201:enzymology; D006801:Humans; D007231:Infant, Newborn; D008214:Lymphocytes / Q000201:enzymology*; D013050:Spectrometry, Fluorescence / Q000379:methods
D000123:Acetyltransferases