Tritium (3H) radiolabeling of protein A and antibody to high specific activity: application to cell surface antigen radioimmunoassays.
Staphylococcal protein A and several different immunoglobulins have been radiolabeled to high specific activities (greater than 10(6) cpm/microgram) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D000954:Antigens, Surface; D001666:Binding Sites, Antibody; D001894:Borohydrides / Q000494:pharmacology; D006041:Goats; D007074:Immunoglobulin G / Q000378:metabolism; D007553:Isotope Labeling; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011817:Rabbits; D011863:Radioimmunoassay; D013205:Staphylococcal Protein A / Q000378:metabolism*; D014316:Tritium
D000906:Antibodies; D000954:Antigens, Surface; D001894:Borohydrides; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D014316:Tritium