title: Polynucleotides. LVII. Synthesis and properties of poly (2'-chloro-2'-deoxyinosinic acid).
abstract: Poly (2'-chloro-2'-deoxyinosinic acid) [poly(Icl)] was synthesized from Icl 5'-DP by polymerization with polynucleotide phosphorylase. UV absorption properties of poly(Icl) are very similar to those of poly(I). Poly(Icl) adopted a multi-stranded ordered form in the presence of 0.95M Na ion. The Tm value of this form was 36 degrees, which resembles that of poly(I) quadruple-stranded form at high salt. CD spectra also suggested presence of these two forms. Upon mixing with poly(C), poly-(Icl) forms a double-stranded 1 : 1 complex, which had very similar Tm-log[Na+] relationship to that of poly(I) . poly(C). Thus it was concluded that the chlorine substitution at 2'-position of the polynucleotide had the similar effect to OH on physical properties of polynucleotides.
mesh_terms: D002942:Circular Dichroism; D004355:Drug Stability; D006358:Hot Temperature; D009690:Nucleic Acid Conformation; D011066:Poly C; D011069:Poly I / Q000138:chemical synthesis; D011117:Polyribonucleotide Nucleotidyltransferase; D011131:Polyribonucleotides / Q000138:chemical synthesis; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011131:Polyribonucleotides; D011069:Poly I; D011066:Poly C; D011117:Polyribonucleotide Nucleotidyltransferase