Inhibition of Tamm-Horsfall glycoprotein induction of alkaline phosphatase in cystic fibrosis fibroblasts by medium conditioned by normal cells.
It is confirmed that the level of alkaline phosphatase in fibroblasts derived from cystic fibrosis patients can be induced many-fold by growing the cells in the presence of Tamm-Horsfall glycoprotein. It is further shown that normal fibroblasts produce a "CF corrective factor" which markedly inhibits this phenomenon. These observations support a previous hypothesis on the nature of the metabolic defect in cystic fibrosis.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D002478:Cells, Cultured / Q000378:metabolism*; D003470:Culture Media; D003550:Cystic Fibrosis / Q000201:enzymology / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D013997:Time Factors
D003470:Culture Media; D006023:Glycoproteins; D000469:Alkaline Phosphatase