Triple-spot proteins in two-dimensional gel electrophoresis.
A triple-spot pattern of polypeptides occurring in two-dimensional gel electrophoresis of proteins is described. The presence of a mutant protein, Pc 1 Duarte, which results in a splitting of all three polypeptides, is evidence that they are produced by the same gene. This pattern is seen in about 1% of the proteins from a variety of sources. Typically, about 50% of the protein occurs as a single major spot, the remainder occurring as two polypeptides with an additional negative charge and slightly different molecular weight. The reproducibility of this pattern implies a functional significance which is presently unknown. The implication of this configuration for patterns seen by one-dimensional gel electrophoresis is discussed.
D000818:Animals; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D006801:Humans; D008970:Molecular Weight; D009154:Mutation; D010455:Peptides / Q000302:isolation & purification; D011487:Protein Conformation; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats
D010455:Peptides; D011506:Proteins