Acetylcholine does not stimulate the release of growth hormone from perifused rat adenohypophyses.
Acetylcholine (1 micromol/L--1 mmol/L) has been reported to stimulate growth hormone release from perifused bovine pituitary slices. We have carried out studies using the perifused pars distalis of the rat adenohypophysis to see whether a similar response is observed. Neither acetylcholine, acetylcholine with eserine, nor carbamylcholine, at concentrations of 10(-9), 10(-6), and 10(-3) M. stimulated the release of growth hormone. Thus we could not demonstrate a similar reponse to acetylcholine in the pars distalis of the rat adenohypophysis as reported in the bovine gland.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D013006:Growth Hormone / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010830:Physostigmine / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D013045:Species Specificity
D002217:Carbachol; D013006:Growth Hormone; D010830:Physostigmine; D000109:Acetylcholine