Influence of L-tyrosine phenol-lyase on the growth and metabolism of B16 melanoma.
Administration of L-tyrosine phenol-lyase inhibited growth of established B16 melanomas in BDF1 mice. Under the conditions used, the enzyme exerted a cytostatic effect on the tumors. The addition of L-tyrosine phenol-lyase to growing melanoma cultures blocked cell division and caused an accumulation of cells in the G1 (G0) pultures was suppressed, the most pronounced effect of L-tyrosine phenol-lyase was a rapid and near-complete cessation of uridine uptake and incorporation. This block in RNA synthesis was also observed in human fibroblasts treated with L-tyrosine phenol-lyase.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D008190:Lyases / Q000627:therapeutic use*; D008545:Melanoma / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014447:Tyrosine Phenol-Lyase / Q000627:therapeutic use*
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D008190:Lyases; D014447:Tyrosine Phenol-Lyase