Radioimmunoassay for pyrazolone derivatives.
The determination of pyrazolone derivatives by radioimmunoassay has been reported. Anti-antipyrine antisera were obtained by repeated immunization of rabbits with 4-azoantipyrine-conjugated bovine serum albumin. The level at least as low as 1.0 ng of antipyrine could be detected by this procedure. Various substituents on the carbon 4 position of the pyrazolone ring as well as the lack of methyl group on the nitrogen 2 position decreased the affinity for the antibody, and the antibody showed no cross-reaction with any pyrazolidine derivatives.
D000675:Ampyrone / Q000032:analysis; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000983:Antipyrine / Q000031:analogs & derivatives / Q000032:analysis; D001666:Binding Sites, Antibody; D002417:Cattle; D003429:Cross Reactions; D007106:Immune Sera / Q000494:pharmacology; D008297:Male; D011720:Pyrazoles / Q000032:analysis*; D011817:Rabbits; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000276:immunology
D007106:Immune Sera; D011720:Pyrazoles; D000675:Ampyrone; D012710:Serum Albumin, Bovine; D000983:Antipyrine