Assay of the binding of the ternary complex Met-tRNAf.eIF-2.GTP to the 40 S ribosomal subunit by sucrose gradient and CsCl gradient analysis.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002499:Centrifugation, Density Gradient / Q000379:methods; D006160:Guanosine Triphosphate / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D046911:Macromolecular Substances; D008715:Methionine; D051379:Mice; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D046911:Macromolecular Substances; D010448:Peptide Initiation Factors; D006160:Guanosine Triphosphate; D012343:RNA, Transfer; D008715:Methionine