A study of the muscarinic receptor by gel electrophoresis.
1 Muscarinic receptors from the brain of rat, guinea-pig and frog have been labelled with tritiated propylbenzilylcholine mustard ([3H]-PrBCM). 2 After solubilisation and sodium dodecyl sulphate (SDS)-polycarylamide gel electrophoresis a single radiolabelled peak was seen which was completely suppressed when labelling was carried out in the presence of 10(-6) M atropine. The results suggest the presence of a single polypeptide chain with a molecular weight of similar to or approximately 80,000. 3 In the case of intestinal smooth muscle multiple labelled peaks were found. Precautions against proteolysis led to the identification of a major peak with a molecular weight in the region of 80,000.
D000818:Animals; D001001:Anura; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006168:Guinea Pigs; D007082:Ileum / Q000032:analysis; D008861:Microsomes / Q000032:analysis; D011438:Propylbenzilylcholine Mustard; D051381:Rats; D011950:Receptors, Cholinergic / Q000032:analysis*; D011976:Receptors, Muscarinic / Q000032:analysis*; D013570:Synaptic Membranes / Q000032:analysis; D013574:Synaptosomes / Q000032:analysis
D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011438:Propylbenzilylcholine Mustard