[Metabolically stable classes of nuclear messenger-like RNA. I. Detection and molecular characterization].
Two discreet in size molecular classes of metabolically stable messenger-like RNA molecules with sedimentation coefficientes about 28S and 18S have been revealed in the nuclei of pigeon bone marrow cells. The structural pecularities of 28S RNA class were investigated more carefully. It was shown to constitute the largest fraction of nuclear messenger-like RNA and is characterized by a GC/AU ratio 1.13. As pulse-labeled nuclear RNA, 28S stable nuclear RNA hybridizes with the unique and rare repeated DNA, it does not contain poly(A)-sequences and is found in a form of RNP particles with density of 1.41 g/sm3, tightly bound to chromatin.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D010856:Columbidae; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D012265:Ribonucleotides / Q000032:analysis
D012333:RNA, Messenger; D012265:Ribonucleotides; D012313:RNA
Metabolicheski stabil'nye kalssy iadernoi RNK informatsionnogo tipa. I. Vyiavlenie i molekuliarnaia kharakteristika.