title: Microspectrofluorometric analysis of surface antigens of murine melanoma and hamster peritoneal cell hybrids: comparisons of species antigenicity, chromosome number, and tumorigenicity.
abstract: Somatic cell hybrids from viral fusions of murine melanoma (PAZG) X Chinese hamster peritoneal cells (CH) were compared with respect to surface antigenicity, karyotype and tumorigenicity. One line, F57-(9), which arose from the hybridization of two CH cells and one PAZG cell, had slight (6%) CH chromosome loss but 80%PAZG chromosome loss after 10 months in culture. These cells expressed CH antigens strongly and PAZG antigens weakly. In comparison, another hybrid, F57-(7), formed from one CH and one PAZG cell, lost 20% of its chromosomes after 10 months in vitro. These cells had a stronger expression of PAZG antigens and weaker expression of CH antigens than F57-(9). These findings indicate a direct relationship between chromosome number and antigenicity; tumorigenicity, however, does not appear to depend on the chromosome numbers of the parental cells.
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm; D000954:Antigens, Surface / Q000032:analysis*; D002460:Cell Line; D002875:Chromosomes; D006224:Cricetinae; D006822:Hybrid Cells; D008545:Melanoma / Q000235:genetics / Q000276:immunology; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology; D013045:Species Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface