title: Gonadotrophin determination in 24-h-urine by sensitive LH and FSH radioimmunoassays.
abstract: Urinary Lutropin (LH) and Follitropin (FSH) were precipitated with acetone and measured by specific radioimmunoassays. Recovery experiments with special regard to urinary pH values were done by adding 125I-labelled LH and FSH to unprocessed urine. At pH 4.5 68.6% of LH and 66.1% of FSH were recovered. Increasing pH values up to 6.0 resulted in a significant recovery loss of 125-I-LH, but not of 125I-FSH. Immunoreactive FSH concentrations decreased significantly 6 and 48 hours after storage began at room temperature. In healthy males and females we found mean LH and FSH concentrations in 24-h-urine which were similar to those reported by others: LH: males: 22.6 IU/24-h; cycling females: 17.8 IU/24-h; postmenopausal females: 49.1 IU/24-h; FSH: males 6.0 IU/24-h: cycling females: 15.0 IU/24-h; postmenopausal females: 48.5 IU/24-h. Because of their high sensitivity and practicability, we believe that these radioimmunoassays are clinically useful approaches in assessment of pituitary and gonadal disorders in human subjects.
mesh_terms: D004355:Drug Stability; D005260:Female; D005640:Follicle Stimulating Hormone / Q000652:urine*; D005498:Follicular Phase; D006801:Humans; D007986:Luteinizing Hormone / Q000652:urine*; D008297:Male; D008593:Menopause; D008832:Microchemistry; D011863:Radioimmunoassay / Q000379:methods; D012737:Sex Factors
chemical_list: D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone