Effect of hormones on primary cell cultures from pregnancy-dependent mouse mammary tumors.
No hormone combination was successful in maintaining long term primary cultures of pregnancy-dependent mammary tumors. Insulin provided the most consistent dose dependent stimulatory effect on short term proliferation as measured by 3H-TdR incorporation into DNA. Insulin in combination with corticosterone and prolactin produced the greatest stimulatory effect in most tumors. Both insulin at 5 microgram/ml and insulin, prolactin and hydrocortisone induced a partially synchronous wave of DNA synthesis in restimulated cultures. The time course of the wave of DNA synthesis was different for different hormone treatments. Insulin as 5 microgram/ml caused an earlier wave of DNA synthesis than insulin, prolactin plus corticosterone.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003345:Corticosterone / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006728:Hormones / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental / Q000503:physiopathology*; D051379:Mice; D011247:Pregnancy; D011270:Pregnancy, Animal; D011374:Progesterone / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology
D004273:DNA, Neoplasm; D006728:Hormones; D007328:Insulin; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D003345:Corticosterone; D006854:Hydrocortisone