Non-specific immunosuppressive effects of mouse spleen extracts in vitro.
Cell-free extracts were prepared from the spleens of keyhole limpet haemocyanin (KLH)-primed, ovalbumin (OVA)-primed or unprimed mice, and were shown to suppress non-specifically the secondary antibody response to TNP1000KLH in vitro. Non-specific activity was retained on storage in liquid nitrogen and was not removed by dialysis. These findings are in contrast to those reported elsewhere where it was found that cell-free extracts from the spleens of KLH-primed mice specifically suppressed the IgG response to TNP1000KLH; this discrepancy may well lie in the initial immunological status of the mice used. Nevertheless it is concluded that proper account should be taken of non-specific suppressive effects when studying the activity of cell-free extracts in vitro.
D000818:Animals; D000941:Antigens / Q000276:immunology; D002474:Cell-Free System; D002478:Cells, Cultured; D006433:Hemocyanins / Q000276:immunology; D007156:Immunologic Memory; D007165:Immunosuppression Therapy; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000276:immunology*; D014020:Tissue Extracts / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D000941:Antigens; D014020:Tissue Extracts; D014301:Trinitrobenzenes; D010047:Ovalbumin; D006433:Hemocyanins