[The fibrin and plasma-protein content of human thrombi].
The protein composition of cell-free thrombus contained in abdominal aneurysms of the aorta was investigated using PAGE and immunoreactivity after solubilization. 1. The dry weight was 18--29%, with minor differences between various locations. 2. 35--40% of the dry weight was extractable with NaCl/citrate and was identified as albumin (80%) and IgG (15%). Trace amounts of fibrinogen immunoreactive material were present. 3. The NaCl/citrate insoluble part was 80% hydrolyzable with plasmin. The predominant fragment was D-D dimer (from cross-linked fibrin). In addition, fragments Dand E were observed. Reduction of the NaCl/citrate insoluble part with 2-mercaptoethanol resulted in almost complete solubilization. PAGE analysis demonstrated alpha-polmers, gamma-gamma dimers and little free alpha-chains, indicating almost complete cross-linking of fibrin. Thus, the major protein were cross-linked fibrin, albumin and IgG.
D001014:Aortic Aneurysm; D001798:Blood Proteins / Q000032:analysis*; D002474:Cell-Free System; D005337:Fibrin / Q000032:analysis*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis
D001798:Blood Proteins; D007074:Immunoglobulin G; D012709:Serum Albumin; D005337:Fibrin
Der Gehalt menschlicher Thromben an Fibrin und Plasmaproteinen.