Isolation and identification of S(+)-3-hydroxyisobutyric acid in the urine of rats loaded with isobutyric acid.
3-Hydroxyisobutyric acid has been isolated from the urine of rats loaded with sodium isobutyrate. An optical rotation measurement of the methyl ester derivative shows this compound to be the S(+) stereoisomer. This is the same stereoisomer that has been previously identified in cultures of bacteria incubated with ammonium isobutyrate (Aberhart, D.J. (1977) Bioorg. Chem. 6, 191--201).
D000818:Animals; D002087:Butyrates / Q000378:metabolism*; D002849:Chromatography, Gas; D006885:Hydroxybutyrates / Q000302:isolation & purification / Q000652:urine*; D013058:Mass Spectrometry; D051381:Rats; D013237:Stereoisomerism
D002087:Butyrates; D006885:Hydroxybutyrates; C020757:3-hydroxyisobutyric acid