Protein synthesis inhibition induced by dimethylnitrosamine and diethylnitrosamine on isolated rat hepatocytes.
Time- and dose-dependent protein synthesis inhibition takes place following exposure to high doses of dimethylnitrosamine (DMN) or diethylnitrosamine (DENA) in isolated rat hepatocytes. The ability of DENA to depress protein synthesis is 5-fold higher than that of DMN. Cells inhibited by 60 min exposure to DMN or DENA, and then incubated in a nitrosamine-free medium, regain their initial rate of protein synthesis. This recovery is faster and more complete for DENA-treated cells.
D000818:Animals; D004052:Diethylnitrosamine / Q000494:pharmacology*; D004128:Dimethylnitrosamine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009602:Nitrosamines / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D051381:Rats
D009602:Nitrosamines; D004052:Diethylnitrosamine; D004128:Dimethylnitrosamine