Adrenal hydroxylations in genetically obese and hypertensive rats.
The separate steps in the formation of aldosterone from cholesterol were studied in a strain of spontaneously hypertensive rats in which phenotypic obesity is inherited as a recessive trait (Koletsky rats). The obese and hypertensive state had little or no effect on side-chain cleavage of cholesterol, formation of progesterone from pregnenolone or 21-hydroxylation. Mitochondrial 18-hydroxylation of endogenous and exogenous corticosterone, however, as well as 18- and 11 beta-hydroxylation of deoxycorticosterone, were increased in obese hypertensive rats, both when compared with non-obese hypertensive siblings and when compared with healthy Sprague-Dawley rats. 18-Hydroxylation of corticosterone was increased more than 18-hydroxylation of deoxycorticosterone. In non-obese hypertensive rats, the adrenal content of mitochondrial cytochrome P-450 was lower than that in obese hypertensive rats but higher than that in rats of the conventional Sprague-Dawley strain. The results are discussed with respect to possible heterogeneity of adrenal cytochrome P-450 and to possible explanations for the changes observed.
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004195:Disease Models, Animal; D006900:Hydroxylation; D006973:Hypertension / Q000235:genetics / Q000378:metabolism* / Q000662:veterinary; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009765:Obesity / Q000235:genetics / Q000378:metabolism* / Q000662:veterinary; D051381:Rats; D012376:Rodent Diseases / Q000378:metabolism
D003577:Cytochrome P-450 Enzyme System