title: Purification and N-terminal amino acid sequence determination of anionic and cationic canine trypsinogens.
abstract: Anionic and cationic canine trypsinogens were purified from pancreatic juice by affinity chromatography with Trasylol coupled to Sepharose 4B followed by ion exchange chromatography with SP-Sephadex C-50. Automatic N-terminal amino acid sequence determination showed the following structures for the activation peptides: Thr-Pro-Thr-Asp-Asp-Asp-Asp-Lys for anionic trypsinogen, and Phe-Pro-Ile-Asp-Asp-Asp-Asp-Lys for cationic trypsinogen.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004285:Dogs; D006801:Humans; D010189:Pancreatic Juice / Q000201:enzymology; D010446:Peptide Fragments / Q000302:isolation & purification; D013045:Species Specificity; D013552:Swine; D014362:Trypsinogen
chemical_list: D010446:Peptide Fragments; D014362:Trypsinogen