[Modelling of viral-chemical carcinogenesis in chronic infection in mice caused by the herpes simplex type 2 virus].
The influence of chronic herpetic infection of white mice caused by herpes simplex virus type 2 (HSV-2) on the blastomogenic effect of a polycyclic hydro-carbon, 20-methylcholanthrene (20-MCA), was studied. Solid tumors developed in 20-MCA-treated mice on the side of the carcinogen administration within 81--89 days. The simultaneous administration of 70 intracerebral LD50 of HSV-2 subcutaneously into a hind leg pad and 0.1 mg 20-MCA subcutaneously into the inguinal area was accompanied by a synergistic action of the two factors. The number of tumors in the animals treated with HSV-2 in combination with 20-MCA at all intervals of observation was 1.5--2.5 times greater than in mice treated with 20-MCA alone. Mice weighing 10--12 g were more sensitive to the synergistic effect of HSV-2 and 20-MCA, whereas animals weighing 25--30 g were more sensitive to the carcinogenic effect of 20-MCA alone. No tumors developed in mice infected with HSV-2 alone by 167 days (the observation period). Mice weighing 10--12 g were found to be more sensitive to the fatal effect of HSV-2 and HSV-2 plus 20-MCA, particularly in the first 2--3 weeks after inoculation. Six months after inoculation with HSV-2 the virus was isolated from posterior root ganglia of the sacral and lumbar spinal cord by cocultivation of the ganglia with human embryo skin-muscle tissue cells.
D000818:Animals; D002273:Carcinogens; D002908:Chronic Disease; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000382:microbiology; D006561:Herpes Simplex / Q000150:complications*; D008748:Methylcholanthrene; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology*; D013997:Time Factors; D014776:Virus Cultivation
D002273:Carcinogens; D008748:Methylcholanthrene
Modelirovanie virusno-khimicheskogo kantserogeneza v usloviiakh khronicheskoĭ infektsii, vyzvannoĭ virusom prostogo gerpesa tipa 2.