[Diagnosis of type III hyperlipoproteinemia: contribution of an air-driven ultracentrifuge associated with an original tube-slicer (author's transl].
Diagnosis of type III hyperlipoproteinemia requires preparative ultracentrifugation in order to measure the cholesterol/triglyceride molar ratio into isolated d less than 1.006 lipoproteins. The authors describe an ultracentrifugation micromethod which needs 600 microliter of serum and can be completed within three hours (Airfuge Beckman instruments). An original tube-slicer allows the separation of the d less than 1.006 lipoproteins located into top fractions. This simple micromethod might confirm diagnosis of type III in patients and would be useful for clinical laboratories.
D002784:Cholesterol / Q000097:blood; D006801:Humans; D006949:Hyperlipidemias / Q000175:diagnosis*; D008074:Lipoproteins / Q000097:blood; D008722:Methods; D008832:Microchemistry; D014280:Triglycerides / Q000097:blood; D014461:Ultracentrifugation / Q000295:instrumentation*
D008074:Lipoproteins; D014280:Triglycerides; D002784:Cholesterol
Diagnostic de l'hyperlipoprotéinémie de type III: apport d'une ultracentrifugeuse à turbine à air associée à un coupe-tube de conception originale.