[Hexokinase and lactate dehydrogenase activity and isoenzymatic makeup of the soluble fraction of dissimilarly functioning rabbit muscles normally and in experimental allergic encephalomyelitis].
Only one isozyme of hexokinase (type I) was found in a soluble fraction of smooth muscle of rabbit stomach using column chromatography and polyacrylamide gel electrophoresis. By the pattern of metabolism the smooth muscle of rabbit stomach accupies an intermediate position between rapidly and slowly contracting sceletal muscles, approaching to musculus soleus by the activity and isozyme spectra of hexokinase and lactate dehydrogenase (LDH). Activity of these enzymes was altered not uniformly in dissimilarly functioning muscles of rabbits with experimental allergic encephalomyelitis: it was increased in musculus gastrocnemius of rabbits and decreased in soleus or in smooth muscles. LDH isoenzyme spectra changed towards an increase in aerobic H-subunits and decrease in anaerobic M-subunits in the soluble fraction of musculus gastrocnemius of rabbits with experimental allergic encephalomyelitis. Content of LDH-5 and LDH-4 was about 2-fold increased and content of LDH-1 and LDH-2 was decreased in musculus soleus.
D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000201:enzymology*; D004789:Enzyme Activation; D006593:Hexokinase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008297:Male; D009130:Muscle, Smooth / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits; D012995:Solubility; D013270:Stomach
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D006593:Hexokinase
Aktivnost' i izofermentnyĭ sostav geksokinazy i laktatdegidrogenazy rastvorimoĭ fraktsii razlichno funktsioniruiushchikh myshts krolika v norme i pri éksperimental'nom allergicheskom éntsefalomielite.