The excision of N-methyl-N-nitrosourea-induced lesions from the DNA of Chinese hamster cells as measured by the loss of sites sensitive to an enzyme extract that excises 3-methylpurines but not O6-methylguanine.
An enzyme extract from Micrococcus luteus excises 3-methyladenine and 3-methylguanine but not O6-methylguanine, 7-methylguanine, 1-methyladenine or 7-methyladenine from DNA reacted with N-methyl-N-nitrosourea. The extract was used to detect lesions in the DNA of Chinese hamster cells treated in culture with N-methyl-N-nitrosourea. It was concluded that 3-methyladenine is excised from these cells with a half-life of about 2.3 h.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004247:DNA; D003851:Deoxyribonucleases / Q000378:metabolism*; D006147:Guanine / Q000031:analogs & derivatives*; D008770:Methylnitrosourea; D008837:Micrococcus / Q000201:enzymology; D009607:Nitrosourea Compounds; D011687:Purines; D013329:Structure-Activity Relationship
D009607:Nitrosourea Compounds; D011687:Purines; D006147:Guanine; D008770:Methylnitrosourea; D004247:DNA; C008449:O-(6)-methylguanine; D003851:Deoxyribonucleases