Immobilized enzyme nylon tube reactors: creatininase and creatine kinase linked to the pyruvate kinase-lactate dehydrogenase indicator system in the detection of creatinine and creatine.
A four enzyme system catalyzing a sequence of reactions was immobilized onto the inside surfaces of nylon tubes and studied as a possible method of analysis for the first substrate, creatinine in the sequence of reactions. The system was first studied in solution as a guide to optimizing conditions for preparation and use of the immobilized enzyme nylon tube reactors. The scope and limitations of this approach to routine analysis is discussed.
D000581:Amidohydrolases / Q000378:metabolism*; D003401:Creatine / Q000032:analysis*; D003402:Creatine Kinase / Q000378:metabolism*; D003404:Creatinine / Q000032:analysis*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008722:Methods; D009757:Nylons; D011770:Pyruvate Kinase / Q000378:metabolism*
D004800:Enzymes, Immobilized; D009757:Nylons; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D011770:Pyruvate Kinase; D003402:Creatine Kinase; D000581:Amidohydrolases; C019946:creatininase; D003401:Creatine