Complement activity and conglutinin activity in stored bovine sera.
Complement activity and conglutinin activity were measured in bovine sera stored under four conditions: phenolized (3 C), lyophilized (3 C), frozen (-23 C), and frozen (-57 C). The complement activity was lost after 12 weeks in phenol (3 C). The other three conditions, ranked in order of increasing effectiveness, are lyophilized (3 C) and frozen (-23 and -57 C). Conglutinin activity was lost after 23 weeks in phenol (3 C). The other three conditions, ranked in order of increasing effectiveness, are frozen (-23 C), lyophilized (3 C) and frozen (-57 C). Thus, the most effective condition to preserve complement and conglutinin simultaneously is freezing at -57 C.
D000818:Animals; D001793:Blood Preservation / Q000662:veterinary*; D001798:Blood Proteins / Q000032:analysis; D002417:Cattle / Q000276:immunology*; D003165:Complement System Proteins / Q000032:analysis*; D005260:Female; D005612:Freeze Drying; D005615:Freezing; D008297:Male; D010636:Phenols / Q000494:pharmacology; D013696:Temperature
D001798:Blood Proteins; D010636:Phenols; D003165:Complement System Proteins