Potentiation of antiboty-dependent cell-mediated cytotoxicity by target cell-bound C3b.
Antibody-dependent cytolytic effector lymphocytes are known to possess, in part, receptors for activated C3. Employing a model system consisting of 51Cr-labeled chicken erythrocytes and purified human peripheral lymphocytes, we investigated the effect of target cell bound C3b on antibody-dependent cellular cytotoxicity (ADCC). At concentrations of anti-target cell antibody too low to cause effective ADCC, target cell bound C3b cooperated with antibody to produce marked target cell lysis. In the presence of a 1/6.25 X 10(6) dilution of anti-chicken erythrocyte rabbit IgG, cell lysis increased from 20% to 65% by the attachment of 18,000 C3b molecules per cell. C3b-dependent enhancement of ADCC was dose dependent. It was augmented by attachment of activated properdin (P) to the C3b-bearing target cells.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000920:Antibody-Dependent Cell Cytotoxicity; D002645:Chickens; D003176:Complement C3 / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D011414:Properdin / Q000276:immunology; D011817:Rabbits
D000906:Antibodies; D003176:Complement C3; D011414:Properdin