Inhibition of human alkaline phosphatases by vanadate.
Orthovanadate was shown to be a potent competitive inhibitor (Ki less than 1 microM) of purified alkaline phosphatase from human liver, intestine of kidney. Inhibition was reversed and full enzymic activity restored in the presence of 1mM-adrenaline. Phosphate and vanadate competed for the same binding site on the enzyme.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D006801:Humans; D007421:Intestine, Small / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D010710:Phosphates / Q000494:pharmacology; D014639:Vanadium / Q000494:pharmacology*
D010710:Phosphates; D014639:Vanadium; D000469:Alkaline Phosphatase