title: Synthesis and characterization of a new fluorescent phospholipid.
abstract: A novel fluorescent phospholipid, whose structure was tentatively assigned as 1-(2'-thio-1'-hydroxyethyl)-2-(ethylphosphatidyl)isoindole), was synthesized by reacting O-phthalaldehyde and beta-mercaptoethanol with phosphatidylethanolamine. The fluorescent lipid product was purified by silicic acid chromatography. The purity was demonstrated by thin-layer chromatography. This fluorescent phospholipid could not form stable lipid vesicles. However, a mixture of phosphatidylcholine and this fluorescent phospholipid did form stable vesicles after sonication, as demonstrated by Sepharose 4B column chromatography and electron microscopy. The absorption and fluorescence properties of this lipid, both as aqueous micelles or incorporated into vesicles, have been determined. The potential usage of this new fluorescent phospholipid in membrane studies is discussed.
mesh_terms: D005456:Fluorescent Dyes; D054833:Isoindoles; D007700:Kinetics; D008854:Microscopy, Electron; D010743:Phospholipids / Q000138:chemical synthesis; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
chemical_list: C021098:1-(2'-thio-1'-hydroxyethyl)-2-(ethylphosphatidyl)isoindole; D005456:Fluorescent Dyes; D054833:Isoindoles; D010743:Phospholipids