title: Complement-mediated binding of immunoglobulins to some endocrine cells of the pancreas and gut.
abstract: Unspecific binding of immunoglobulins to gastrin, somatostain and glucagon cells of the gastrointestinal mucosa or pancreas has been found to occur through a nonantigen-antibody mechanism mediated by the C14 fraction of complement. The phenomenon represents an important drawback in hormone immunohistochemistry, which can be overcome by using complement deprived, highly dilute anti-hormone sera.
mesh_terms: D000818:Animals; D001665:Binding Sites; D003165:Complement System Proteins / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D007136:Immunoglobulins; D007413:Intestinal Mucosa / Q000276:immunology*; D010179:Pancreas / Q000276:immunology*; D011817:Rabbits; D013552:Swine
chemical_list: D007136:Immunoglobulins; D003165:Complement System Proteins