Plasmenylethanolamine: growth factor for cholesterol-reducing Eubacterium.
A plasmalogen, plasmenylethanolamine, is required for in vitro growth of strains of Eubacterium which convert cholesterol to coprostanol. Plasmenylethanolamine was isolated from calf brain by selective saponification of lipid fractions separated by thin-layer or column chromatography. Cholesterol-containing thioglycolate broth plus purified plasmenylethanolamine or its 2-lyso derivative supported growth of Eubacterium ATCC 21408 and a cholesterol-reducing Eubacterium isolated from baboon feces. Plasmenylethanolamine obtained from commercial sources also supported growth of these organisms, but none of a number of other pure lipids would support growth. Metabolism of the alkenyl ether group of plasmenylethanolamine occurred during growth.
D002784:Cholesterol / Q000378:metabolism*; D005051:Eubacterium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D007700:Kinetics; D010955:Plasmalogens / Q000494:pharmacology*
D010955:Plasmalogens; C020791:phosphatidal ethanolamines; D002784:Cholesterol