title: Sickled erythrocytes, hyphema, and secondary glaucoma: IV. The rate and percentage of sickling of erythrocytes in rabbit aqueous humor, in vitro and in vivo.
abstract: The number of erythrocytes adopting the sickled configuration in rabbit aqueous was determined with a masked counting technique in vitro and in vivo. The percentage of cells that became sickled in aqueous humor was greater than that observed in a control salt solution and in the donor blood itself. Aqueous humor, therefore, can be considered a particularly deleterious medium for erythrocytes having a propensity for sickling. Blood samples from six donors with systemically severe hemoglobinopathies (SC, Sthal, and SS) had relatively high levels of hemoglobin S and, in general, showed faster and more frequent sickling than did blood samples from donors with low levels of hemoglobin S (one SS patient, whose chronic blood transfusion regimen had replaced most of his hemoglobin S with normal hemoglobin, and two AS patients). Even erythrocytes from sickle cell trait cases, however, were capable of sickling when immersed in acqueous humor.
mesh_terms: D000755:Anemia, Sickle Cell / Q000150:complications*; D000818:Animals; D000867:Anterior Chamber; D001082:Aqueous Humor / Q000502:physiology*; D004913:Erythrocytes, Abnormal; D006451:Hemoglobin, Sickle; D006801:Humans; D006988:Hyphema / Q000150:complications*; D066298:In Vitro Techniques; D007267:Injections; D011817:Rabbits; D012805:Sickle Cell Trait / Q000150:complications*
chemical_list: D006451:Hemoglobin, Sickle