A microcytotoxicity assay using 51Cr for measuring specific cell-mediated and humoral immune responses.
A microcytotoxicity assay (MCA) for measuring in vitro cell-mediated cytotoxicity (CMC) and humoral cytotoxicity which uses 51Cr-labelled target cells is described. Data is presented to show the uptake and rentention of 51Cr by various viable cultured cells. The CMC determined by 51Cr-labelled target cell retention in microtest II plate wells was verified by microscopic observation. Comparisons between the CMC observed 24 and 48 h after incubation of immune and normal lymphoid cells with 51Cr-labelled target cells were made. The factors, including savings in time and expense, which may make this modified MCA superior to other MCAs in use, are discussed.
D000818:Animals; D000906:Antibodies; D002448:Cell Adhesion; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003165:Complement System Proteins / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D005260:Female; D007111:Immunity, Cellular; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000276:immunology; D009447:Neuroblastoma / Q000276:immunology; D011392:Proline / Q000494:pharmacology; D011817:Rabbits
D000906:Antibodies; D002860:Chromium Radioisotopes; D003165:Complement System Proteins; D011392:Proline