Automated determination of total plasma cholesterol: a serum calibration technique.
The automated (AutoAnalyzer II) determination of cholesterol in nine serum pools in the concentration range 3.465-8.871 mmol/l, gave results that were approximately 10% higher than reference values when the analyses were based on unesterified cholesterol standards containing the same amount of water as the sample extracts (1963 analyses in 12 laboratories during a 12 month period; automated value = 0.032 + 1.10 X (Reference value)). A serum calibration procedure was successful ilues, and was equally effective in correcting the values observed for aliquots of 368 fresh-frozen plasma samples analyzed in each of the 12 laboratories during a 38 month period.
D001322:Autoanalysis; D002784:Cholesterol / Q000097:blood*; D005069:Evaluation Studies as Topic; D005615:Freezing; D006801:Humans; D008722:Methods; D012016:Reference Values
D002784:Cholesterol