The comparative effectiveness of the "brown and blue reactions" for tracing neuronal processes of cells injected intracellularly with horseradish peroxidase.
The comparative advantages and disadvantages of diaminobenzidine (DAB) and benzidine di--HCl (BDHC) procedures for tracing processes of cells injected intracellularly with horseradish peroxidase (HRP) have been studied. The DAB reaction gives a good definition of membranes and appears more suitable for visualizing the fine structure of dendrites. The BDHC procedure, being more sensitive, induces crystal formation and heavily filled processes appear frosted. This last procedure is, however, much more appropriate for tracing the axonal system of injected cells.
D015100:3,3'-Diaminobenzidine / Q000378:metabolism*; D000818:Animals; D001560:Benzidines / Q000378:metabolism*; D001931:Brain Mapping / Q000379:methods*; D002415:Cats; D006651:Histocytochemistry / Q000379:methods; D006735:Horseradish Peroxidase / Q000378:metabolism*; D009044:Motor Cortex / Q000033:anatomy & histology / Q000166:cytology* / Q000378:metabolism; D009046:Motor Neurons / Q000166:cytology; D010544:Peroxidases / Q000378:metabolism*; D011712:Pyramidal Tracts / Q000033:anatomy & histology
D001560:Benzidines; D015100:3,3'-Diaminobenzidine; D006735:Horseradish Peroxidase; D010544:Peroxidases