Dihydropyrimidine amidohydrolase is a zinc metalloenzyme.
Bovine liver dihydropyrimidine amidohydrolase (EC 3.5.2.2) has been subjected to atomic absorption analysis. Three different preparations of homogeneous enzyme indicated that the enzyme contains 4.3 +/- 0.3 g atoms of Zn2+ per mol of enzyme or 1.1 g atoms of Zn2+ per subunit. No Co2+, Mn2+, Mg2+ or Cd2+ was detected. Exhaustive dialysis against either o-phenanthroline or EDTA did not reduce enzyme activity; however, prolonged incubation with dipicolinic acid resulted in inactivation which can be reversed by either Zn2+ or Co2+ but not Mg2+.
D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D008099:Liver / Q000201:enzymology*; D008667:Metalloproteins; D013054:Spectrophotometry, Atomic; D014498:Uracil / Q000031:analogs & derivatives; D015032:Zinc / Q000378:metabolism*
D008667:Metalloproteins; D014498:Uracil; D000581:Amidohydrolases; D015032:Zinc