Biological and biochemical criteria for the characterization of the transformed stage of BHK21 cells.
Some quantitative criteria for spontaneous and virus-induced transformation of BHK21 cells are described. The resistance to acid polysaccharides such as dextran sulphate in agarose medium is a characteristic shared by all tumorigenic clones. The increase in a high molecular weight integral membrane protein seems to be a biochemical feature correlated with this property.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D006224:Cricetinae; D003911:Dextrans / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007668:Kidney; D008565:Membrane Proteins / Q000096:biosynthesis; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000096:biosynthesis
D003911:Dextrans; D007328:Insulin; D008565:Membrane Proteins; D009363:Neoplasm Proteins