[3H-thymidine incorporation into the macrophages in the process of eye regeneration in adult tritons].
Changes in the number of labelled macrophages in the regenerating eye cavity of the adult common newts were studied within 2 to 12 days following the injection of 3H-thymidine and removal of retina and lens from the eyes. A small number of labelled macrophages was found in the eye cavity (0,2--5.9%) at all regeneration stages under study upon the pulse 3H-thymidine incorporation. Their number rapidly increased and attained by the end of observation (12 days) 73%. These results suggest that mainly mature non-dividing forms of macrophages which completed the mitotic cycle S-phase in the places of their active reproduction migrate in the eye cavity. The sharp increase of the number of labelled macrophages in the eye cavity is determined by the migration of those macrophages the precursors of which were labelled as a result of both the pulse 3H-thymidine incorporation and reutilization of the labelled precursors of DNA synthesis. New portions of labelled macrophages migrate in the eye cavity within 2 to 4 days.
D000818:Animals; D002455:Cell Division; D002465:Cell Movement; D007908:Lens, Crystalline / Q000502:physiology; D008264:Macrophages / Q000378:metabolism*; D009799:Ocular Physiological Phenomena; D012038:Regeneration; D012160:Retina / Q000502:physiology; D013936:Thymidine / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium; D014319:Triturus / Q000502:physiology*
D014316:Tritium; D013936:Thymidine
Vkliuchenie 3H-timidina v makrofagi v protsesse regeneratsii glaza u vzroslykh tritonov.