Isolation of human C1q as a by-product of routine fractionation.
Clq can be isolated as a by-product of large scale fractionation. The euglobulins of fraction III are submitted to chromatography on CM-cellulose in a batch-procedure. The active fraction being frequently contaminated by lysozyme the protein can be further purified by gel-filtration in the presence of carbohydrates. The purified Clq is suitable for the detection of immune complexes.
D005591:Chemical Fractionation; D002845:Chromatography; D003172:Complement C1 / Q000302:isolation & purification*; D006801:Humans; D008722:Methods
D003172:Complement C1