Immunization of mice against coccidioidomycosis with a subcellular vaccine.
In vitro-cultivated spherules of Coccidioides immitis were disrupted in a Braun homogenizer. The resultant wall fragments were separated from cell sap and endospores by centrifugation. Extraction of the walls with phosphate-buffered saline yielded a subcellular fraction that was immunogenic in mice. This fraction, which contained polysaccharide and protein, gave substantial protection against intranasal challenge with a usually lethal dose (1,000) of arthrospores, provided that it was administered with adjuvant.
D000276:Adjuvants, Immunologic; D000818:Animals; D002473:Cell Wall / Q000276:immunology; D002688:Chitinases / Q000494:pharmacology; D003045:Coccidioides / Q000276:immunology* / Q000648:ultrastructure; D003047:Coccidioidomycosis / Q000517:prevention & control*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005657:Fungal Vaccines; D008297:Male
D000276:Adjuvants, Immunologic; D005657:Fungal Vaccines; D002688:Chitinases