Treatment of L5178Y tumor-bearing BDF1 mice with a nonimmunogenic L-glutaminase-L-asparaginase.
An L-glutaminase-L-asparaginase from Achromobacter has been rendered nonimmunogenic by the covalent attachment of polyethylene glycol (PEG) to nonessential amine groups of the enzyme. PEG-L-glutaminase-L-asparaginase exhibits a greatly enhanced half-life in the bloodstream compared to the unmodified enzyme in normal mice, and is effective in prolonging the survival of BDF1 mice inoculated ip with L5178Y cells. PEG-L-glutaminase-L-asparaginase appears rapidly in the blood following ip injection.
D000581:Amidohydrolases / Q000008:administration & dosage* / Q000097:blood / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D001215:Asparaginase; D001216:Asparagine; D004359:Drug Therapy, Combination; D005972:Glutaminase; D005973:Glutamine; D008657:Metabolic Clearance Rate; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D011092:Polyethylene Glycols
D005973:Glutamine; C022324:polyethylene glycol-glutaminase-asparaginase; D011092:Polyethylene Glycols; D001216:Asparagine; D000581:Amidohydrolases; D001215:Asparaginase; D005972:Glutaminase