Spectrophotometric assay for amikacin using purified kanamycin acetyltransferase.
A rapid spectrophotometric assay has been developed for measuring the concentrations of amikacin and related antibiotics in serum. The assay uses a purified enzyme from R-factor E. coli which acetylates amikacin with the production of coenzyme A, the latter in turn being reacted with a sulfhydryl reagent to produce stoichiometric amounts of a sensitive chromophore, that is measured in the visible spectrum. The system complements an earlier assay for gentamicin-related antibiotics thereby facilitating the rapid measurement of the concentrations of all clinically important aminoglycosides in serum.
D000123:Acetyltransferases; D000583:Amikacin / Q000097:blood*; D007612:Kanamycin / Q000031:analogs & derivatives* / Q000378:metabolism; D008722:Methods; D013053:Spectrophotometry
D007612:Kanamycin; D000583:Amikacin; D000123:Acetyltransferases