Combined histochemical and biochemical investigation to the reliability of the demonstration of arylsulphatase activity in cryostat sections.
The reliability of the enzyme histochemical technique, for the demonstration of arylsulphatase activity, using 6-bromo-2-naphthylsulphate as a substrate, is biochemically tested by using partly purified lysosome and microsome preparations from fresh human placenta tissue. Microsomes from frozen placenta with an arylsulphatase deficiency and lysosomes from rat liver, are also investigated. For the biochemical test methods, 6-bromo-2-naphthylsulphate and p-nitrocatecholsulphate are used as substrates. Under similar reaction conditions, varying the pH of the incubation medium and adding inhibitors or activators, the histochemical and biochemical reactions are compared. The results of this study show that the enzyme histochemical technique--except for some limitations--is suitable for the demonstration of microsomal arylsulphatase in cryostat sections.
D000818:Animals; D001192:Arylsulfatases / Q000172:deficiency / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism; D002458:Cell Fractionation; D005260:Female; D005629:Frozen Sections; D006801:Humans; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D009281:Naphthalenes / Q000378:metabolism; D010920:Placenta / Q000166:cytology / Q000201:enzymology*; D011247:Pregnancy; D051381:Rats; D013429:Sulfatases / Q000378:metabolism*
D002396:Catechols; D009281:Naphthalenes; D013429:Sulfatases; D001192:Arylsulfatases