title: Decreased binding of drugs and dyes to plasma proteins from rats with acute renal failure: effects of ureter ligation and intramuscular injection of glycerol.
abstract: 1 The decreased binding of drugs and dyes to plasma proteins from male and female rats with acute renal failure has been investigated using equilibrium dialysis at 37 degrees C. 2 Acute renal failure induced by bilateral ligation of the ureters produced a greater than threefold increase in the unbound fraction of o-methyl red relative to normal rat plasma. Unbound dye concentration in plasma from sham-operated control rats was also significantly increased but to a lesser extent. 3 Glycerol-induced acute renal failure produced a significant increase in the unbound fractions of o-methyl red, methyl orange, bromocresol green (BCG), 2-(4'-hydroxybenzeneazo) benzoic acid (HABA), phenytoin and salicylic acid. A marginally significant increase in unbound warfarin concentration was observed. 4 Glycerol-induced renal failure had no effect on total plasma protein concentration and experiments with o-methyl red and salicylic acid indicated that a direct effect of glycerol was not responsible for the diminution of binding. 5 Glycerol-induced acute renal failure, which produced decreases in drug and dye binding similar to those reported for human uraemic plasma, provides a convenient non-surgical animal model for the investigation of this phenomenon.
mesh_terms: D058186:Acute Kidney Injury / Q000097:blood* / Q000139:chemically induced; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism*; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005990:Glycerol; D008297:Male; D010672:Phenytoin / Q000097:blood*; D051381:Rats; D012459:Salicylates / Q000097:blood*; D012737:Sex Factors; D014508:Urea / Q000097:blood; D014513:Ureter / Q000502:physiology*; D014859:Warfarin / Q000097:blood*
chemical_list: D001798:Blood Proteins; D004396:Coloring Agents; D012459:Salicylates; D014859:Warfarin; D010672:Phenytoin; D014508:Urea; D003404:Creatinine; D005990:Glycerol