[Analysis of brain chromatin subunit composition using different endonucleases].
A comparison of the processes of chromatin digestion in brain and liver nuclei by Ca, Mg-dependent and staphylococcal endonucleases demonstrates a similarity of the subunit composition of chromatin from both tissues and reveals the same type of linked DNA regions. However, a formation of low molecular weight DNP fragments during hydrolysis and the DNA spectra of soluble and insoluble DNP fragments suggest that brain chromatin contains these fragments alongside with the regions, which are specific for this particular tissue, predominate in it and are resistant to staphylococcal and, particularly, to Ca, Mg-dependent endonucleases. This is paralleled with a non-histone protein enrichment of different brain chromatin fractions and an expansion of the electrophoretic monomer band towards the fragment with a greater molecular weight. It may be assumed that brain nucleosomes are characterized by a higher size heterogeneity of linked DNA, part of which are mostly covered by non-histone proteins, and/or are characterized by a greater set variety.
D000818:Animals; D001923:Brain Chemistry; D002843:Chromatin / Q000032:analysis*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D004247:DNA / Q000032:analysis*; D003851:Deoxyribonucleases; D003852:Deoxyribonucleoproteins / Q000032:analysis*; D004720:Endonucleases; D008297:Male; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis*; D051381:Rats
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D003852:Deoxyribonucleoproteins; D009698:Nucleoproteins; D004247:DNA; D003851:Deoxyribonucleases; D004720:Endonucleases
Analiz syb'edinichnoĭ organizatsii khromatina mozga s pomoshch'iu razlichnykh endonykleaz.