title: Peripheral lymphocytes changes in the anamnestic response to tetanus toxoid challenge.
abstract: Few data are available on the blood lymphocyte response to revaccination in man. The anamnestic response to tetanus toxoid challenge was evaluated by a variety of techniques during the first week after revaccination. Out of twenty subjects used, eight were evaluated before and 5 days after the injections (days 1--8). Analytical cell electrophoresis showed important variation in the B and two T lymphocyte populations. The B cell percentages, assessed by EAC-rosettes and electrophoretic mobility, were found to decrease by days 2 and 3, and return to former levels by day 8, when a rise in specific antibodies was detected. A similar response was found in the T1 population generally considered to be composed of low affinity E-rosette-forming cells. Conversely, a significant increase (50--100%) in circulating T2 lymphocytes (active rosette-forming cells) was found by days 2 and 3, followed by a rapid decrease of these 'differenciated' cells. The increase in the T2 lymphocytes appeared earlier in skin test positive subjects. These changes were correlated with E-rosettes, mitogen stimulation, peripheral leucocyte migration inhibition and transformation in the presence of the antigen. EA-IgG rosettes and ADCC varied similarly. These results may indicate a significant non-specific cell mobilization following revaccination.
mesh_terms: D000920:Antibody-Dependent Cell Cytotoxicity; D002464:Cell Migration Inhibition; D004586:Electrophoresis; D006801:Humans; D007117:Immunization, Secondary; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D012397:Rosette Formation; D012882:Skin Tests; D013745:Tetanus Toxoid / Q000276:immunology*
chemical_list: D013745:Tetanus Toxoid