[Fluorometric determination of "activated" cyclophosphamide and ifosfamide in blood (author's transl)].
"Activated" N-(2-Chloroethyl)amido-oxazaphosphorines like 4-hydroxycyclophosphamide, 4-hydroperoxycyclophosphamide, 4-hydroxyifosfamide, and 4-hydroperoxyifosfamide can be determined fluorometrically by condensation of liberated acrolein with m-aminophenol yielding 7-hydroxychinolin. The method permits determination of 10(-10) mol and is specific for "activated" N-(2-Chloroethyl)amido-oxazaphosphorine metabolites which liberate acrolein under conditions of the test. Neither cyclophosphamide nor ifosfamide or other metabolites of this cytostatics interfere with the test. Blood levels of free 4-hydroxycyclophosphamide and 4-hydroxyifosfamide were determined after injection of cyclophosphamide and ifosfamide into mice.
D000171:Acrolein; D000627:Aminophenols; D000818:Animals; D003520:Cyclophosphamide / Q000097:blood*; D005470:Fluorometry; D007069:Ifosfamide / Q000097:blood; D051379:Mice
D000627:Aminophenols; D000171:Acrolein; D003520:Cyclophosphamide; D007069:Ifosfamide
Fluorometrische Bestimmung von "aktiviertem" Cyclophosphamid und Ifosfamid im Blut.