Studies of nuclear proteins in the skeletal muscle of the cardiomyopathic Syrian hamster.
Nonhistone nuclear proteins (NHNP) were isolated from the skeletal muscle of dystrophic hamsters and their respective paired controls. These proteins were extracted with phenol and fractionated by polyacrylamide gel electrophoresis. One-dimensional gel electrophoresis using either sodium-dodecylsulfate or isoelectrofocusing revealed quantitative changes between the two groups. An improved resolution using a two-dimensional system showed both quantitative and very limited qualitative differences. There are differences in proteins focusing at pH 5.0, molecular weight 55,000, in the dystrophic tissue. In addition proteins from dystrophic muscle focusing between pH 5.0 and 7.0, molecular weight 45,000, and proteins focusing between pH 7.0 and 9.0, molecular weights 35,000--45,000 and 60,000--70,000 were reduced as compared to the controls. There were no detectable differences in the electrophoretic patterns between the two groups of proteins derived from skeletal muscle homogenates. The differences in NHNP appear to be reflections of alterations in the nuclear composition of the dystrophic muscle cell. Some of these differences may represent changes secondary to the muscle disease. However, if NHNP interacting with DNA play a major role in the control of genetic expression, some of the manifestations of hamster dystrophy could be due to a different constitution of NHNP in the skeletal muscle.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007525:Isoelectric Focusing; D008647:Mesocricetus; D008854:Microscopy, Electron; D008970:Molecular Weight; D009132:Muscles / Q000032:analysis* / Q000648:ultrastructure; D009136:Muscular Dystrophies / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate
D002868:Chromosomal Proteins, Non-Histone; D012967:Sodium Dodecyl Sulfate