title: L-Glutamine formation by Flavobacterium rigense.
abstract: A penicillin-resistant mutant of Flavobacterium rigense designated as strain 703, FERM-P no. 3628, was obtained after ultraviolet treatment of F. rigense FERM-P no. 3556. The parent strain produces 0-2-hydroxypropylhomoserine from 1,2-propanediol. The mutant was found to be a good producer of L-glutamine. The physiological characteristics of strain 703 were different from the general group of L-glutamic acid-producing bacteria. Strain 703 required L-tryptophan and thiamine but not biotin for its growth. L-Glutamine formation on a specific basis, however, was independent of L-tryptophan and thiamine. Biotin and penicillin were also not effective. Only ammonium fumarate acted as an effective factor on L-glutamine formation. Accumulation of L-glutamine by strain 703 was 10 mg/ml at 30 degrees C for 48 h in a chemically defined medium containing 3% diammonium fumarate.
mesh_terms: D005417:Flavobacterium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism; D005973:Glutamine / Q000096:biosynthesis*; D009154:Mutation; D010403:Penicillin Resistance; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013237:Stereoisomerism; D013831:Thiamine / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism
chemical_list: D005650:Fumarates; D000644:Quaternary Ammonium Compounds; D005973:Glutamine; D014364:Tryptophan; D013831:Thiamine