Bioconversion and biosynthesis of 16-membered macrolide antibiotics. XIII. Regulation of spiramycin I 3-hydroxyl acylase formation by glucose, butyrate, and cerulenin.
The effects of glucose, butyrate, and cerulenin on the formation of spiramycin I 3-hydroxyl acylase were investigated by using the cell-free extract prepared from the spiramycin-producing strain of Streptomyces ambofaciens KA-1028. Glucose induced the formation of the acylase, and this induction was remarkably repressed by butyrate. Cerulenin, on the other hand, not only cancelled the repression by butyrate but also stimulated the formation of the acylase. The unsuccessful trapping of spiramycin I as an intermediate during the bioconversion from neospiramycin I to spiramycin III in the presence of cerulenin was due to the rapid acylation of spiramycin I caused by the acylase induced by cerulenin.
D000217:Acyltransferases / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000935:Antifungal Agents / Q000494:pharmacology*; D001711:Biotransformation; D002087:Butyrates / Q000494:pharmacology*; D002474:Cell-Free System; D002569:Cerulenin / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D007933:Leucomycins / Q000096:biosynthesis*; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D002087:Butyrates; D007933:Leucomycins; D002569:Cerulenin; D000217:Acyltransferases; D005947:Glucose