Genetic microsurgery by laser: establishment of a clonal population of rat kangaroo cells (PTK2) with a directed deficiency in a chromosomal nucleolar organizer.
An ultraviolet laser beam was focused to a submicron spot on one of the nucleolar organizer regions of mitotic chromosomes of rat kangaroo cells in tissue culture. The daughter cells were isolated and cloned into a viable population that maintained the directed nucleolar deficiency. It is concluded that the laser can be used to delete preselected genetic regions and the genetic deletion is maintained as a heritable deficiency in subsequent daughter cells.
D000818:Animals; D002460:Cell Line; D002466:Cell Nucleolus / Q000648:ultrastructure; D002871:Chromosome Banding; D002875:Chromosomes / Q000528:radiation effects; D002999:Clone Cells / Q000648:ultrastructure; D007668:Kidney; D007834:Lasers; D007614:Macropodidae; D009697:Nucleolus Organizer Region / Q000528:radiation effects*; D010641:Phenotype; D014466:Ultraviolet Rays