Application of the parallel line bioassay method to quantitative determination of the HBs antigen in radioimmunoassay.
Precise and reproducible quantitative determination of hepatitis B surface antigen by applying the parallel line bioassay method was proposed. In this method, the value of any given sample can be expressed in terms of a relative value to a fixed standard or reference preparation. Decrease in antigenic reactivity due to the lowered protein content could be prevented by using 0.02% human albumin in physiological saline solution as the diluent. The use of such stabilizer(s) should be considered in the dilution or storage of samples containing HBs antigen.
D001681:Biological Assay / Q000379:methods*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D012044:Regression Analysis
D006514:Hepatitis B Surface Antigens; D011933:Reagent Kits, Diagnostic