Effect of sodium chloride on limulus amebocyte lysate. Inhibition of endotoxin activation of procoagulase.
Concentrations of sodium chloride up to 3 M increase the time necessary for the clot formation from Limulus amebocyte lysate (LAL) induced with endotoxin. Sodium chloride at a concentration of 4 M prevents clot formation by either precipitation or denaturation of procoagulase. The time necessary for the activation of procoagulase by endotoxin is increased by a change in the sodium chloride concentration from 0.15 M to 0.588 M. No effect on the proteolytic phase or the polymerization phase of the clotting reaction is detected by the increase in sodium chloride concentration from 0.15 M to 0.588 M. The authors conclude that increased sodium chloride concentrations may aid the isolation of procoagulase.
D000818:Animals; D001777:Blood Coagulation; D003030:Coagulase / Q000378:metabolism*; D003864:Depression, Chemical; D004731:Endotoxins / Q000037:antagonists & inhibitors*; D008033:Limulus Test; D012965:Sodium Chloride / Q000494:pharmacology*; D014357:Trypsin / Q000494:pharmacology
D003030:Coagulase; D004731:Endotoxins; D012965:Sodium Chloride; D014357:Trypsin