title: Sequential degradation of a chondroitin sulphate trisaccharide by lysosomal enzymes from embryonic-chick epiphysial cartilage.
abstract: The disulphated trisaccharide D-N-acetylgalactosamine sulphate-beta-D-glucuronic acid-beta-D-N-acetylgalactosamine sulphate prepared from 35S- or 14C-labelled chondroitin sulphate was incubated with a preparation of lysosomal enzymes from embryonic-chick epiphysial cartilage. Degradation was demonstrated by analysis of the reaction products. By use of the appropriate intermediate products as substrates, in conjunction with specific enzyme inhibitors, it was shown that the degradation proceeded sequentially from the non-reducing end. It was initiated by sulphatase (preferentially hydrolysing sulphate ester groups at the 6-position), followed by beta-N-acetylgalactosaminidase and beta-glucuronidase, converting the substrate into monosaccharides and inorganic sulphate. The latter enzyme preferentially attacked disaccharides carrying their sulphate ester group at C-4 of the hexosamine residue. Generation of chondroitin sulphate oligosaccharides may occur by the action of an endoglycosidase, previously demonstrated in embryonic-chick cartilage. Endo- and exo-enzymes may thus form a functional unit in lysosomal degradation of chondroitin sulphate.
mesh_terms: D000818:Animals; D002356:Cartilage / Q000201:enzymology*; D002642:Chick Embryo; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D004838:Epiphyses / Q000201:enzymology; D005966:Glucuronidase / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology*; D009844:Oligosaccharides / Q000378:metabolism*; D013429:Sulfatases / Q000378:metabolism; D014312:Trisaccharides / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D014312:Trisaccharides; D002807:Chondroitin; D002809:Chondroitin Sulfates; D013429:Sulfatases; D006596:Hexosaminidases; D005966:Glucuronidase