title: Electrophoresis of glucocerebrosidase from normal and Gaucher disease fibroblasts.
abstract: A polyacrylamide gel electrophoresis system for glucocerebrosidase has been developed. This method was used to characterize the glucocerebrosidase activity of normal and Gaucher disease fibroblasts; the residual glucocerebrosidase activity in adult Gaucher disease fibroblasts co-migrates with the activity from normal fibroblasts.
mesh_terms: D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000201:enzymology; D005776:Gaucher Disease / Q000201:enzymology*; D005959:Glucosidases / Q000032:analysis*; D005962:Glucosylceramidase / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques
chemical_list: D005959:Glucosidases; D005962:Glucosylceramidase