The production of antibodies to vitamin B-12.
The conjugation of vitamin B-12 to HSA by means of a combined ethylcarbodiimide N-hydroxysuccinimide conjugation method at pH 5.5 is described. It gave a ratio of 2.7 mol vitamin B-12/mol HSA. Five rabbits were immunized with this conjugate emulsified in Freund's complete adjuvant. After one booster injection high titre antisera (1 : 450 000--1 : 1 500 00) to vitamin B-12 were collected.
D000818:Animals; D000917:Antibody Formation; D002234:Carbodiimides; D055598:Chemical Phenomena; D002621:Chemistry; D006241:Haptens; D007106:Immune Sera; D007114:Immunization; D011817:Rabbits / Q000276:immunology; D012709:Serum Albumin; D013388:Succinimides; D014805:Vitamin B 12 / Q000276:immunology*
D002234:Carbodiimides; D006241:Haptens; D007106:Immune Sera; D012709:Serum Albumin; D013388:Succinimides; D014805:Vitamin B 12