[Dissociation of human factor VIII and recombination of the fragments VIII-C and VIII-AG].
Human factor VIII has been dissociated into fragment VIII-C and fragment VIII-AG in the presence of calcium chloride (0.25 M). After removing the calcium, recombination of the molecule is obtained. Fragment VIII-C can be activated by thrombin in a similar manner to the coagulant activity of whole factor VIII. This activation is calcium-dependent. In rabbits immunized against purified F VIII, VIII-C and VIII-AG specific antisera against these three entities were obtained.
D001780:Blood Coagulation Tests; D002118:Calcium; D002122:Calcium Chloride / Q000494:pharmacology; D002623:Chemistry Techniques, Analytical; D005169:Factor VIII; D006801:Humans; D007106:Immune Sera; D008623:Mercaptoethanol; D009500:Neutralization Tests; D013917:Thrombin
D007106:Immune Sera; D008623:Mercaptoethanol; D005169:Factor VIII; D013917:Thrombin; D002122:Calcium Chloride; D002118:Calcium
Dissociation du facteur VIII humain et recombinaison des fragments VIII-C et VIII-AG.