Fluorimetric assay of acid lipase in human leukocytes.
A fluorimetric assay for leukocyte acid lipase, the enzyme defect in Wolman disease, is described. The method requires a smaller volume of blood and can be performed more rapidly than the present colorimetric assay for the leukocyte enzyme.
D004796:Clinical Enzyme Tests; D005470:Fluorometry / Q000379:methods; D006579:Heterozygote; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D008049:Lipase / Q000097:blood* / Q000172:deficiency; D008064:Lipidoses / Q000175:diagnosis / Q000235:genetics; D014973:Xanthomatosis / Q000175:diagnosis / Q000235:genetics
D008049:Lipase