[Effect of the balanced inhibition of DNA and protein synthesis on the in vitro and in vivo proliferative activity of the lymphocytes].
Lymphocytes isolated from the rabbit peripheral blood were irradiated in vitro with 200, 400 and 1000 r doses and cultivated with phytohemagglutinin (PHA) doses and cultivated with phytohemagglutinin (PHA) at 37 degrees C during 48 hours. In several experiments cycloheximide, inhibiting protein synthesis, was added to the cells 60 minutes before and 30 minutes after irradiation. There was no apparent difference in the viability of irradiated cells with or without cycloheximide. The ability of lymphocytes of the popliteal lymph nodes for proliferation after PHA injection into one of the hind foot-pads of the irradiated mice was studied, as well. The injection of cycloheximide or puromycin into one of the hind foot-pads immediately after irradiation of the animals augmented the proliferation of lymphocytes in this extremity in comparison with contralateral one, 1.5-2 times. Cytosine arabinoside, inhibiting DNA synthesis, was not effective under these conditions.
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011506:Proteins / Q000037:antagonists & inhibitors*; D011691:Puromycin / Q000494:pharmacology; D011817:Rabbits; D013997:Time Factors
D011506:Proteins; D011691:Puromycin; D004247:DNA; D003513:Cycloheximide
Vliianie sbalansirovannogo ingibirovaniia sintezov DNK i belkoa na proliferativnuiu aktivnost' limfotsitov in vitro i in vivo.