Affinity chromatography on immobilized hyaluronate and its application to the isolation of hyaluronate binding properties from cartilage.
Partially degraded hyaluronate was coupled to AH-Sepharose 4B using carbodiimide. Approximately 1 mg of hyaluronate was incorporated per ml of wet gel. The derivatized gel was used to purify components of the hyaluronate-proteoglycan complex of cartilage. Two link-proteins were isolated from a crude cartilage extract by affinity binding to the gel and eluted with 4 M guanidinium chloride. By the same procedure one link-protein and the globular portion of the proteoglycan monomer were isolated from a trypsin-treated cartilage extract and were separated from each other by subsequent gel chromatography on Sepharose 6B and Sephacryl S-200. The affinity technique was also used for the preparation of these proteins labelled with dansyl groups.
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification*; D002356:Cartilage / Q000032:analysis*; D002417:Cattle; D002846:Chromatography, Affinity; D006820:Hyaluronic Acid; D009296:Nasal Cavity; D011509:Proteoglycans / Q000302:isolation & purification; D014357:Trypsin
D002352:Carrier Proteins; D011509:Proteoglycans; D006820:Hyaluronic Acid; D014357:Trypsin