A microsystem for the extraction and in-vitro translation of mouse embryo mRNA.
A micromethod is described for the extraction of mRNA from mouse ova and its translation in a message-dependent rabbit reticulocyte lysate system. Using this technique it has been possible, by SDS polyacrylamide gel fluorography, to visualize the products from the equivalent of 12.5 eggs.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian / Q000032:analysis*; D005260:Female; D066298:In Vitro Techniques; D008715:Methionine / Q000378:metabolism; D008722:Methods; D051379:Mice; D010063:Ovum / Q000032:analysis; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D012333:RNA, Messenger; D008715:Methionine