title: The uptake in vitro of dyes, monosaccharides and amino acids by the filarial worm Brugia pahangi.
abstract: The uptake in vitro of various substances by Brugia pahangi was investigated using infective larvae obtained from Aedes aegypti and worms removed from Meriones unguiculatus at 2, 3, 10, 20 and 90 days post-infection. Worms incubated in growth medium 199 containing 1% Trypan blue possessed demonstrable dye in the oral orifice, the anterior oesophageal lumen and the external openings of the vulva and the cloaca or anus but the dye was not found in the gut lumen even after incubation for 24 h. No uptake of ferritin particles into the intestine of the worms was found and no fluorescence could be demonstrated in the gut lumen of worms incubated in medium containing 50% (v/v) fluorescein isothiocyanate-conjugated calf serum for up to 24 h. Trypan blue uptake by the gut of Aspiculuris tetraptera was clearly observed after incubation for several hours. The uptake of D-glucose and L-leucine by B. pahangi was demonstrated using autoradiographic and scintillation counting techniques and incorporation into worm tissues was detected. Glucose was found to be readily incorporated in the apical, glycogen-rich areas of the myocytes of worms of all ages studied and in the uterine epithelium of the adult female. In contrast, a lower incorporation of D-glucose was found in the eggs, embryos and vas deferens and especially in the gut. The incorporation of L-leucine occurred throughout the tissue of the worms during a 30 min incubation. Labelling was also located over the surface of the cuticle of the worms, when incubated for a period of 15 to 60 min in L-[H]leucine. Scintillation counting techniques demonstrated that there was no uptake of 14C-labelled L-glucose or sucrose by B. pahangi. The data presented on the uptake in vitro of nutrients or other compounds by infective larvae and adult stages of B. pahangi did not demonstrate an intestinal route of uptake but indicated that the transcuticular route of uptake may be employed.
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002009:Brugia / Q000254:growth & development / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D004396:Coloring Agents / Q000378:metabolism*; D005260:Female; D005293:Ferritins / Q000378:metabolism; D005370:Filarioidea / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007930:Leucine / Q000378:metabolism*; D008297:Male; D014343:Trypan Blue / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D004396:Coloring Agents; D005293:Ferritins; D007930:Leucine; D014343:Trypan Blue; D005947:Glucose