[CIEP with cellogel (cellulose acetate membrane) in the diagnosis of visceral leishmaniosis (author's transl)].
Technique of counter immunoelectrophoresis (CIEP) was employed for the diagnosis of V.L. (human and canine) using strips of cellulose acetate and an antigen grossly extracted (by means of repeated freezing and thawing) from culture of Leishmania. 4 lots of antigen was prepared, in various time, at the same way. Positive results were obtained in V.L. from 81 to 90% (according to various lots of antigen). False positivities (from 1 to 4.5%) occur in patient with other diseases (especially cirrhosis and blood disorders). None positivity in controls (blood donors). Present results and those obtained with same technique in agar, suggested the validity of method and encouraged the production of antigen in a "kit" form for use in the field.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000941:Antigens; D002482:Cellulose; D011232:Chemical Precipitation; D003378:Counterimmunoelectrophoresis / Q000379:methods*; D004285:Dogs; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D002482:Cellulose
La controimmunoelettroforesi (CIEP) su membrana di acetato di cellulosa (Cellogel) nella diagnostica della leishmaniosi viscerale.