Inhibitory action of follicular fluid on progesterone and prostaglandin synthesis in bovine follicles.
The present studies examined the inhibitory effect of mid-cycle and preovulatory bovine follicular fluid (bFF) on the secretion of progesterone and prostaglandin F 2 alpha (PGF 2 alpha) by bovine theca and granulosa cells cultured in tissue culture Medium 199. The inhibitory effect of bFF on the secretion of PGF 2 alpha by incubated and cultured hemipituitary glands of male rats was also studied. Addition of 5, 10 or 20% charcoal-extracted, mid-cycle bFF to the culture medium caused a twofold decrease in the accumulation of both progesterone and PGF 2 alpha by the cultured granulosa cells (P less than 0.01). In contrast, when preovulatory bFF was used, there was no inhibitory effect on secretion of progesterone and PGF 2 alpha. The addition of 10% mid-cycle bFF to the culture medium caused a two- to fourfold decrease in the accumulation of both PGF 2 alpha and progesterone by the cultured theca (p less than 0.008). However, in the presence of 1 microgram LH/ml, the inhibitory effect of mid-cycle bFF on progesterone and PGF 2 alpha secretion was abolished. The secretion of PGF 2 alpha was significantly (p less than 0.03) decreased in male rat hemipituitary glands after 5 h of incubation or 18 h of culture. These findings suggest that bFF from mid-cycle follicles inhibits prostaglandin synthetase as well as luteinization. The inhibition disappeared with bFF from preovulatory follicles.
D000818:Animals; D001826:Body Fluids / Q000502:physiology; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D006080:Ovarian Follicle / Q000378:metabolism* / Q000502:physiology; D010902:Pituitary Gland / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis* / Q000378:metabolism; D011460:Prostaglandins F / Q000096:biosynthesis* / Q000378:metabolism; D013799:Theca Cells / Q000378:metabolism*
D011460:Prostaglandins F; D011374:Progesterone