Inhibition of renal gluconeogenesis in rats by ochratoxin.
In kidney-cortex slices from rats fed on 2.0 mg of ochratoxin A/kg per day for 2 days, gluconeogenesis from pyruvate is decreased by 26%, and renal phosphoenolpyruvate carboxykinase activity is lowered by about 55%. Gluconeogenesis from 10 mM-lactate or 20 mM-malate or -glutamine is also significantly decreased. Hepatic phosphoenolpyruvate carboxykinase is unchanged or increased, and hexokinase activity in kidney and liver remains unaffected. We conclude that ochratoxin A in vivo is an inhibitor of renal phosphoenolpyruvate carboxykinase activity, which is responsible, at least in part, for the block in renal gluconeogenesis.
D000818:Animals; D005943:Gluconeogenesis / Q000187:drug effects; D006593:Hexokinase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects; D007672:Kidney Cortex / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009793:Ochratoxins / Q000494:pharmacology*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D051381:Rats
D009793:Ochratoxins; D006593:Hexokinase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)