[Metabolism of colostomized laying hens with 15N-labeled wheat. 2. Incorporation of the labeled nitrogen into fractions of the egg].
The colostomised hens received 15N-labelled wheat. For a period of 8 days the incorporation of the 15N excess (15N') into the albumen, the yolk and the egg-shell was measured. In addition to that, the values for atom-percent of 15N' in the amino acids lysine, histidien and arginine of the albumen and the yolk were ascertained. The egg-shell and the albumen were labelled shortly after the beginning of the experiment. In both fractions the atom-per cent of 15N' rises more quickly than in the yolk. After the withdrawal of the labelled feed the decrease in the yolk was slower than in the egg-shell and the albumen. Concerning the atom-per cent of 15N' there is hardly a difference between the total N and the amino acids. Despite the different supply with amino acids, the relation of histidine 15N' : lysine 15N' : arginine-15N' in the yolk and the albumen remains largely constant. It is 1 : 2 : 3 and corresponds to the quantitative relation of the N content of the three amino acids in the egg protein. The utilisation of 14N and 15N' of the wheat lysin for the egg synthesis amounts to approximately 50%. There are considerable differences between the utilisation of 15N' of the histidine and 15N' of the arginine and the 14N of the two dietary amino acids.
D000596:Amino Acids / Q000032:analysis; D000821:Animal Feed; D000818:Animals; D003125:Colostomy; D005260:Female; D007553:Isotope Labeling; D009587:Nitrogen Isotopes; D010058:Oviposition; D010063:Ovum / Q000032:analysis*; D014908:Triticum
D000596:Amino Acids; D009587:Nitrogen Isotopes
Stoffwechseluntersuchungen mit 15N-markiertem Weizen bei kolostomierten Legehennen. 2 Mitteilung. Inkorporation des markierten Stickstoffes in die Eifraktionen.