Differences in [3H]-thymidine uptake of lymph node cells stimulated by Con A and PHA in H-2 congenic mouse strains.
The genetic control of the lymphocyte responsiveness to Con A and PHA-P has been studied by using inbred, H-2 congenic mouse strains. Segregation studies were carried out on F1 and backcross mice of a high and a low responder strain. According to the results, there is a strong correlation between the responsiveness of lymph node cells to mitogens and H-2 haplotypes in different H-2 congenic strains of mice and in different backcross generations. High responsiveness to PHA-P is associated with H-2b, while low responsiveness with H-2a or H-2k haplotypes, but the correlation is the inverse in response to Con A, in the parental strains and backcross mice as well. The magnitude of the responses to both Con A and PHA-P was found to be intermediate in F1 heterozygotes of a low and high responder parental strain. The effect of other non H-2 genes on the responsiveness to these mitogens has also been demonstrated.
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006183:H-2 Antigens / Q000235:genetics*; D066298:In Vitro Techniques; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics / Q000276:immunology; D008938:Mitosis / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism*
D006183:H-2 Antigens; D037102:Lectins; D003208:Concanavalin A; D004247:DNA; D013936:Thymidine