Subcellular localization of the enzyme that forms mannosyl retinyl phosphate from guanosine diphosphate [14C]mannose and retinyl phosphate.
The subcellular distribution of the enzyme catalysing the conversion of retinyl phosphate and GDP-[14C]mannose into [14C]mannosyl retinyl phosphate was determined by using subcellular fractions of rat liver. Purity of fractions, as determined by marker enzymes, was 80% or better. The amount of mannosyl retinyl phosphate formed (pmol/min per mg of protein) for each fraction was: rough endoplasmic reticulum 0.48 +/- 0.09 (mean +/- S.D.); smooth membranes (consisting of 60% smooth endoplasmic reticulum and 40% Golgi apparatus), 0.18 +/- 0.03; Golgi apparatus, 0.13 +/- 0.03; and plasma membrane 0.02.
D000818:Animals; D004224:Diterpenes; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008360:Mannosephosphates / Q000096:biosynthesis; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011103:Polyisoprenyl Phosphate Monosaccharides / Q000096:biosynthesis*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D014801:Vitamin A / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism
D004224:Diterpenes; D008360:Mannosephosphates; D009702:Nucleoside Diphosphate Sugars; D011103:Polyisoprenyl Phosphate Monosaccharides; D011105:Polyisoprenyl Phosphate Sugars; D011106:Polyisoprenyl Phosphates; D011506:Proteins; D014801:Vitamin A; D006155:Guanosine Diphosphate Mannose; C013101:mannosylretinylphosphate