Detection of a mediator derived from endotoxin-stimulated macrohpages that induces the acute phase serum amyloid A response in mice.
The mechanism by which LPS stimulates an acute phase serum amyloid A (SAA) response in C3H mice has been studied. A factor (SAA inducer) appears in the blood of C3H/HeN (lipopolysaccharide [LPS]-sensitive) mice approximately 1 h after administration of LPS, which, when passively administered, can induce C3H/HeJ mice to produce SAA although they are resistant to the LPS itself. SAA inducer has been detected in the culture medium of LPS treated C3H/HeN macrophages but not spleen cells. Thus, two stages in the induction of the acute phase SAA response are now recognized: a latent period of 2-3 h during which the SAA concentration remains at baseline values and in which SAA inducer appears, and the period of synthesis of SAA which lasts for approoximately 24 h past induction. It is proposed that a macrophage response to LPS is responsible for production of the serum mediator which induces SAA synthesis.
D000682:Amyloid / Q000096:biosynthesis*; D000818:Animals; D002678:Chimera; D004731:Endotoxins / Q000494:pharmacology*; D005260:Female; D007116:Immunization, Passive; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D000685:Serum Amyloid A Protein / Q000096:biosynthesis*; D013154:Spleen / Q000276:immunology; D013997:Time Factors
D000682:Amyloid; D004731:Endotoxins; D008070:Lipopolysaccharides; D000685:Serum Amyloid A Protein