Decarboxylation of ornithine and lysine in rat tissues.
The possibility that arginine and lysine might be decarboxylated by rat tissues was investigated. No evidence for decarboxylation of arginine could be found. Lysine decarbosylase (L-lysine carboxy-lyase, EC 4.1.1.18) activity producing CO2 and cadaverine was detected in extracts from rat ventral prostate, androgen-stimulated mouse kidney, regenerating rat liver and livers from rats pretreated with thioacetamide. These tissues all have high ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) activities. Lysine and ornithine decarboxylase activities were lost to similar extents on inhibition of protein synthesis by cycloheximide and on exposure to alpha-difluoromethylornithine. A highly purified ornithine decarboxylase preparation was able to decarboxylate lysine and the ratio of ornithine to lysine decarboxylase activities was constant throughout purification. Kinetic studies of the purified preparation showed that the V for ornithine was about 4-fold greater than for lysine, but the Km for lysine (9 mM) was 100-times greater than that for ornithine (0.09 mM). These experiments indicate that all of the detectable lysine decarboxylase activity in rat and mouse tissues was due to the action of ornithine decarboxylase and that significant cadaverine production in vivo would occur only when ornithine decarboxylase activity is high and lysine concentrations substantially exceed those of ornithine.
D000818:Animals; D001120:Arginine / Q000378:metabolism; D002103:Cadaverine / Q000096:biosynthesis; D002245:Carbon Dioxide / Q000096:biosynthesis; D002262:Carboxy-Lyases / Q000378:metabolism*; D003653:Decarboxylation; D005260:Female; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008239:Lysine / Q000378:metabolism*; D008297:Male; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D065108:Ornithine Decarboxylase Inhibitors; D011467:Prostate / Q000201:enzymology; D051381:Rats
D065108:Ornithine Decarboxylase Inhibitors; D002245:Carbon Dioxide; D001120:Arginine; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D008239:Lysine; D002103:Cadaverine