Multiple forms of epidermal alpha-fucosidase.
Two forms of alpha-fucosidase from newborn rat epidermis were separated by gel filtration on Sephadex G-150. These enzymes termed, fucosidase I which was eluted in the void volume and fucosidase II (molecular weight approximately 50,000). Both enzymes had pH optima for 4-methylumbelliferyl-alpha-L-fucoside hydrolysis between 5.5-6.5. Km values for fucosidase I and II with the same substrate were 3.7 X 10(-5) and 5.4 X 10(-5) M, respectively. Three different forms of new born rat epidermal alpha-fucosidase were separated by isoelectric focusing. Evidence is present which indicates that the electrophoretic heterogeneity of alpha-fucosidase is due in part to the binding of sialic acid to the primary gene product.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D004817:Epidermis / Q000201:enzymology*; D051381:Rats; D005644:alpha-L-Fucosidase
D005644:alpha-L-Fucosidase