title: 4-Hydroxy-3-nitrophenylglyoxal. A chromophoric reagent for arginyl residues in proteins.
abstract: The chromophoric reagent, 4-hydroxy-3-nitrophenylglyoxal, is highly selective for the modification of arginine in aqueous solution at pH 7--9. The reagent also inactivates creatine kinase (ATP:creatine N-phosphotransferase, EC 2.7.3.2) in a manner analogous to that reported with phenylglyoxal.
mesh_terms: D000447:Aldehydes; D001120:Arginine / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D003402:Creatine Kinase / Q000037:antagonists & inhibitors*; D007202:Indicators and Reagents; D010658:Phenylglyoxal / Q000031:analogs & derivatives / Q000138:chemical synthesis; D011506:Proteins / Q000032:analysis
chemical_list: D000447:Aldehydes; D007202:Indicators and Reagents; D011506:Proteins; D001120:Arginine; D003402:Creatine Kinase; D010658:Phenylglyoxal