[Identification of the amino acids participating in interaction with DNA in the makeup of a viral nucleoprotein].
A method is described for identification of amino acids participating in covalent DNA-protein cross-links formed in the course of modification of native CD phage by soldium bisulfite. The method is based on estimation of increased content of free amino acids, formed after treatment of the isolated cross-link products using equimolar mixture of O-methyl hydroxylamine and sodium bisulfite. Thin layer chromatography on the plates Fixion 50 x 8 was used for comparative semi-quantitative analysis of amino acids.
D000596:Amino Acids / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004279:DNA, Viral / Q000032:analysis*; D005609:Free Radicals; D009698:Nucleoproteins / Q000032:analysis*; D011485:Protein Binding; D014764:Viral Proteins / Q000032:analysis
D000596:Amino Acids; D004279:DNA, Viral; D005609:Free Radicals; D009698:Nucleoproteins; D014764:Viral Proteins
Ob identifikatsii aminokislot, uchastvuiushchikh vo vzaimodeĭstvii s DNK v sostave virusnogo nukleoproteida.