title: Cultivation of dengue virus type 2 in baby hamster kidney cells in serum-free medium.
abstract: Propagation of dengue virus type 2 (New Guinea C strain) was performed using a shaker culture of baby hamster kidney cells (BHK-21) cultivated in serum-free modified Waymouth medium. Maximum virus titer varied from 10(8.3) to 10(8.8) plaque forming units/ml after incubation of BHK-21 cells in suspension culture at 37 dgrees C for 40-48 hours post-infection.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D003716:Dengue Virus / Q000254:growth & development*; D007668:Kidney; D014776:Virus Cultivation
chemical_list: D003470:Culture Media