title: [Coelomic gonadal epithelia during the period of human embryonic morphologic sex differentiation].
abstract: The course of divergent differentiation of coelomic epithelium in the indifferent gonad has been traced up to the stage when morphological sex signs are distinctly seen. Eleven human embryos fixed in Carnoy and Bouin's fluid have been studied from 6 to 10 weeks of gestation. Paraffin sections were stained with hematoxylin, ferric hematoxylin after Heidenhain. Basal membrane fibres were revealed by silver impregnation after Karupu. PAS-reaction after McManus and additional staining with hematoxylin and light green were applied, as well as the reaction for total proteins with bromphenol blue after Miguel--Calvo. Sex differentiation of coelomic epithelium in the gonad developing according to the male type begins a little later than in the ovary. In the epithelium of the forming testis a great amount of mitotic figures is observed that might be connected with the presence of Y-chromosome stimulating cells for extra mitotic cycles. The increasing presence of Y-chromosome stimulating cells for extra mitotic cycles. The increasing number of mitotic figures in the epithelium should be considered as a sign demonstrating that differentiation according to the male type has started. Superficial epithelium of the embryonic ovary has a peculiar false pseudostratified structure. This secures cellular reserve for the ovarian cortex formation from the external epithelium. The apical surface of the cells in the external epithelium has a border which is evidently formed by microvilli and revealed by PAS-reaction and bromphenol blue. Ovarian follicular cells and Sertoli's cells (sustentocytes) in testis have the common origin in the human fetus--from coelomic epithelium of the gonad germ.
mesh_terms: D004847:Epithelial Cells; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008938:Mitosis; D010053:Ovary / Q000196:embryology* / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012671:Seminiferous Tubules / Q000196:embryology; D012733:Sex Differentiation; D013737:Testis / Q000196:embryology* / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D011506:Proteins
vernacular_title: Tselomicheskiĭ épiteliĭ gonad v period morfologicheskoĭ differentsirovki pola u zarodysheĭ cheloveka.