title: Scanning molecular sieve chromatography of interacting protein systems. III. Effect of kinetic parameters on the large zone boundary profiles for local equilibration between mobile and stationary phases.
abstract: Large zone reaction boundary profiles for molecular sieve chromatography as affected by kinetic parameters have been simulated for local equilibration between the mobile and stationary phases. Our studies of monomer-dimer and monomer-tetramer systems indicate that in a slowly equilibrating system, the kinetic controls operating between the mobile and stationary phases contribute most significantly to the overall boundary profile. In a rapidly equilibrating system, however, the kinetic parameters kij and kji operating in the mobile phase are the principal determinants of the reaction boundary, while the kinetic effects of kii and k-ii between the mobile and stationary phases are minimal.
mesh_terms: D002850:Chromatography, Gel / Q000379:methods; D007700:Kinetics; D046911:Macromolecular Substances; D008433:Mathematics; D011506:Proteins / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D046911:Macromolecular Substances; D011506:Proteins