Mutagenic effect on L5178Y mouse lymphoma cells by growth in ethylene oxide-sterilized polycarbonate flasks.
The mutation frequency of L5178Y mouse lymphoma cells to resistance to 5'-bromo-2'-deoxyuridine increased 6- to 14-fold after growth in ethylene oxide-sterilized polycarbonate culture flasks compared to growth in glass flasks. No comparable increase was observed when L5178Y cells were grown in identical polycarbonate culture flasks sterilized by autoclaving.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002460:Cell Line; D046508:Culture Techniques / Q000295:instrumentation*; D004351:Drug Resistance; D005027:Ethylene Oxide; D005898:Glass; D008223:Lymphoma; D051379:Mice; D009154:Mutation; D011108:Polymers; D013242:Sterilization
D011108:Polymers; D001973:Bromodeoxyuridine; D005027:Ethylene Oxide