Myosin synthesis in embryonic chicken fibroblasts.
The rate of constitutive myosin synthesis was measured in cultures of replicating embryonic chicken skin fibroblasts by pulse labeling with [3H]leucine. These cells synthesized the 200,000-dalton heavy chain of myosin (MHC) at a rate of 3.2 x 10(3) molecules/cell/min. Additionally, an independent estimate of the MHC synthesis rate needed to maintain a constant level of constitutive MHC/cell was calculated from total protein content, percentage MHC, fibroblast doubling time, and MHC half-life. This calculated rate of approximately 2.9 x 10(3) molecules/cell/min was in close agreement with the measured rate. By comparison, the synthesis rate of myofibrillar MHC in fully activated muscle cell cultures was approximately 2.9 x 10(4) molecules/nucleus/min.
D000818:Animals; D002455:Cell Division; D002642:Chick Embryo; D046508:Culture Techniques; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D008970:Molecular Weight; D009132:Muscles; D009210:Myofibrils / Q000378:metabolism; D009218:Myosins / Q000096:biosynthesis*; D012867:Skin
D009218:Myosins