Plasma lipid analysis by thin-layer chromatography with flame ionisation detection and quantitation.
A method is presented whereby cholesterol, cholesterol ester, triacylglycerol, non-esterified fatty acid and polar lipid in plasma may be simultaneously quantitated. Following the addition of umbelliferyl palmitate as internal standard, plasma (0.1 ml) is extracted with chloroform/methanol (2 : 1, v/v). A portion of the extract is chromatographed on silica gel fused to a narrow cylindrical quartz rod. After chromatography the rod is traversed through a flame ionisation detector and the responses recorded and integrated. The procedure is shown to be sensitive, linear, reproducible and to compare well with conventional methods for the determination of plasma lipid classes.
D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood; D002855:Chromatography, Thin Layer / Q000379:methods*; D003124:Colorimetry; D005410:Flame Ionization; D006801:Humans; D008055:Lipids / Q000097:blood*; D008722:Methods; D014280:Triglycerides / Q000097:blood
D002788:Cholesterol Esters; D008055:Lipids; D014280:Triglycerides; D002784:Cholesterol