Brain specific creatine kinase isoenzyme behavior in rat serum after bile acid, sodium oleate and albumin injection.
In this investigation we have shown that severe liver disease with cerebral involvement can be followed by the presence of BB creatine kinase isoenzyme in serum. Groups of rats were injected with a bile acid mixture, oleic acid, albumin and normal saline respectively. Bile acids or/and oleic acid induced BB isoenzyme to leak from central nervous tissue and this can be measured in serum. Albumin binding prevented this leakage. The experiments support our hypothesis, based on biochemical findings in human liver failure, that detergent and surface activity properties of the above mentioned components are able to produce this specific brain isoenzyme leakage.
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001647:Bile Acids and Salts / Q000494:pharmacology*; D001921:Brain / Q000201:enzymology*; D003402:Creatine Kinase / Q000097:blood*; D007527:Isoenzymes; D008297:Male; D009829:Oleic Acids / Q000494:pharmacology*; D011485:Protein Binding; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013499:Surface Properties
D001647:Bile Acids and Salts; D007527:Isoenzymes; D009829:Oleic Acids; D012710:Serum Albumin, Bovine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase