Characterization of protein components of poly(A)-containing messenger ribonucleoproteins from cryptobiotic gastrulae of Artemia salina.
Conditions for the isolation of intact poly(A)+mRNP from cryptobiotic gastruale of A. salina are described. In the presence of Mg2+ ions nucleolytic cleavage occurs in vitro in the vicinity of the 3'-poly(A) segment of mRNP during the isolation procedure. The resulting two parts of poly(A)+mRNP complex are separated by thermal elution from oligo(dT)-cellulsoe affinity column. Analysis by SDS-gel electrophoresis of protein components associated with intact poly(A)+mRNP has revealed the existence of 20--30 S RNP complex containing five major proteins with Mr 68,000, 53,000, 50,000, 45,000 and 38,000, respectively, but completely lacking the poly(A)-specific Mr 76,000 protein.
D000818:Animals; D033364:Decapoda; D004625:Embryo, Nonmammalian; D046911:Macromolecular Substances; D008274:Magnesium; D008970:Molecular Weight; D009698:Nucleoproteins / Q000302:isolation & purification; D011061:Poly A / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification; D012261:Ribonucleoproteins / Q000302:isolation & purification; D012270:Ribosomes / Q000032:analysis
D046911:Macromolecular Substances; D009698:Nucleoproteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D011061:Poly A; D008274:Magnesium