[Enzymological examinations on Clostrium oncolyticum M 55 ATCC 13,732 (author's transl)].
Since excenzymes are mainly involved in oncolysis by Cl. oncolyticum M 55 ATCC 13,732 the enzyme production of the strain should be examined under different growth conditions. The excretion of a protease and of two nucleases was observed in the nutrient medium for 72 h and the results obtained in a nitrogen atmosphere were compared with those obtained in an oxygen atmosphere. The cleavage of protein and of peptides was investigated by means of TLC. The importance of the exoenzymes for the oncolytic effect of Cl. oncolyticum M 55 was confirmed by animal experiments.
D003013:Clostridium / Q000201:enzymology*; D003851:Deoxyribonucleases / Q000378:metabolism*; D009369:Neoplasms / Q000201:enzymology; D010447:Peptide Hydrolases / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism; D013171:Spores, Bacterial
D003851:Deoxyribonucleases; D012260:Ribonucleases; D010447:Peptide Hydrolases
Enzymologische Untersuchungen an Clostridium oncolyticum M 55 ATCC 13.732.