The fate of intratracheally instilled 3H-styrene oxide in the isolated perfused rat lungs.
The metabolism and covalent binding of intratracheally instilled 7-3H-styrene oxide in the isolated perfused lungs of control and cigarette smoke-exposed rats were investigated. After intratracheal instillation of 100 nmoles of 7-3H-styrene oxide the lungs were perfused for 16 min and the nonrecirculating perfusate was collected in two fractions: 0--8 min and 8--16 min. About 40 percent of the instilled dose was in the 0--8 min perfusion medium, mainly in the form of unmetabolized styrene oxide. However, the radioactivity in the 8--16 min perfusion medium and lung tissue itself was mainly in the form of water soluble metabolites, probably glutathione conjugates. The amount of styrene glycol was very small. Exposure of rat to cigarette smoke had no effect on the metabolism of styrene oxide or on the amount of covalent binding to the protein and nucleic acid fractions of the perfused lungs.
D000818:Animals; D066298:In Vitro Techniques; D007442:Intubation, Intratracheal; D008168:Lung / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D012907:Smoking / Q000378:metabolism; D013343:Styrenes / Q000008:administration & dosage / Q000378:metabolism*; D013997:Time Factors; D014132:Trachea / Q000378:metabolism
D013343:Styrenes