Isolation of plasmids from mycobacteria.
A procedure for preparing sodium dodecyl sulfate-salt-cleared lysates of mycobacteria was developed. Standard techniques were employed to demonstrate covalently closed circular deoxyribonucleic acid in lysates of nine strains of Mycobacterium avium-intracellulare.
D004269:DNA, Bacterial / Q000302:isolation & purification*; D004270:DNA, Circular / Q000302:isolation & purification*; D008722:Methods; D009161:Mycobacterium / Q000032:analysis / Q000235:genetics*; D009162:Mycobacterium avium / Q000032:analysis / Q000235:genetics; D010957:Plasmids
D004269:DNA, Bacterial; D004270:DNA, Circular