Automated analysis of hydroxyproline with elimination of non-specific reacting substances.
A modified urinary hydroxyproline method is presented which utilizes an automated technique, Technicon instrumentation and Ehrlich's reagent. Acid hydrolysates are neutralized automatically. Non-specific reacting substances are determined simultaneously with each sample and subtracted as a blank. This method has been found to be superior to the use of charcoal-resin as an absorbent. After blank correction, the mean hydroxyproline excretion of normal and osteoporotic patients are similar. In all groups studied, including Paget's disease, the blanks reresent a significant fraction of total reacting substances and show wide variation between samples. Multiple samples from patients over a two year period show little variation in hydroxyproline excretion when corrected for non-specific reacting substances.
D001322:Autoanalysis; D003124:Colorimetry / Q000379:methods; D006801:Humans; D006909:Hydroxyproline / Q000652:urine*; D007202:Indicators and Reagents; D008297:Male; D010001:Osteitis Deformans / Q000652:urine; D010024:Osteoporosis / Q000652:urine
D007202:Indicators and Reagents; D006909:Hydroxyproline