Monitoring of metabolites applying fibre-entrapped enzymes in a calorimetric system. I -- Glucose and urea determination.
A simple device, consisting of a heat sensor placed in close contact to fibres containing enzymes and connected to a temperature measuring unit has been developed. The system monitors the temperature variations due to the enzymatic reaction when substrate solutions flow through the measuring cell. Fibres containing the enzymes glucose oxidase and catalase for the determination of glucose and fibres containing urease for the determination of urea were tested. A linear relationship between the substrate concentration and the deltaT recorded was obtained in both cases.
D000085:Acetates; D002151:Calorimetry / Q000295:instrumentation / Q000379:methods*; D002374:Catalase; D002482:Cellulose; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D005949:Glucose Oxidase; D014508:Urea / Q000032:analysis / Q000378:metabolism*; D014510:Urease
D000085:Acetates; D004800:Enzymes, Immobilized; D014508:Urea; D002482:Cellulose; D005949:Glucose Oxidase; D002374:Catalase; D014510:Urease; D005947:Glucose