title: Amino acid sequence of the N-terminal non-collagenous segment of dermatosparactic sheep procollagen type I.
abstract: The non-collagenous N-terminal segment of type I procollagen from dermatosparactic sheep skin was isolated in the form of the peptide Col 1 from a collagenase digest of the protein. The peptide has a blocked N-terminus, which was identified as pyrrolid-2-one-5-carboxylic acid. Appropriate overlapping fragments were prepared from reduced and alkylated peptide Col 1 by cleavage with trypsin at lysine, arginine and S-aminoethyl-cysteine residues and by cleavage with staphylococcal proteinase at glutamate residues. Amino acid sequence analysis of these fragments by Edman degradation and mass spectrometry established the whole sequence of peptide Col 1 except for a peptide junction (7--8) and a single Asx residue (44), and demonstrated that peptide Col 1 consists of 98 amino acid residues. The N-terminal portion of peptide Col 1 (86 residues) shows an irregular distribution of glycine, whereas the C-terminal portion (12 residues) possesses the triplet structure Gly-Xy and is apparently derived from the precursor-specific collagenous domain of procollagen. The central region of the peptide contains ten cysteine residues located between positions 18 and 73 and shows alternating polar and hydrophobic sequence elements. The regions adjacent to the cysteine-rich portion have a hydrophilic nature and are abundant in glutamic acid. The data are consistent with previous physicochemical and immunological evidence that distinct regions at the N- and C-termini of the non-collagenous domain possess a less rigid conformation than does the central portion of the molecule.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D003095:Collagen Diseases / Q000378:metabolism / Q000662:veterinary*; D007700:Kinetics; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases; D011347:Procollagen; D011487:Protein Conformation; D012756:Sheep; D012757:Sheep Diseases / Q000378:metabolism*; D012867:Skin / Q000032:analysis*; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D011347:Procollagen; D010447:Peptide Hydrolases; D014357:Trypsin