Studies on the hydrolysis of 1-alk-1'-enyl-sn-glycero-3-phosphoethanolamine by microsomes from myelinating rat brain.
Microsomal fractions of 14-day-old rat brain were incubated at pH 7.1 with 1-[1'-14C]-alk-1'-enyl-sn-glycero-3-phosphoethanolamine (lysoplasmalogen). 1-[1'-14C]alkenylglycerol was produced by hydrolyzing enzyme activities, which were stimulated by Mg2 and inhibited by SH-group reagents. Hydrolysis of 1-[1'-14C]alkyl-sn-glycero-3-phosphoethanolamine is very similar in this respect, but the Km value is higher in the former case. The 1-alkyl compound acts as a non-competitive inhibitor of the hydrolyzing enzyme activity described, whereas the hydrolysis of the 1-alkyl derivative is not inhibited by the 1-alkenyl compound.
D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism*; D009186:Myelin Sheath / Q000378:metabolism*; D010955:Plasmalogens / Q000378:metabolism*; D051381:Rats
D010955:Plasmalogens; D006867:Hydrolases