[Properties of pig liver transketolase].
Some properties of homogeneous transketolase from pig liver were studied. It was shown that the pH optimum of the transketolase reaction lies within the range of 7.8--8.2. The isoelectric point is at pH 7.6--7.8. The molecular weight of transketolase is 138,000 +/- 3,000 as determined by the sedimentation equilibrium method and about 152,000 according to the data from gel filtration through Sephadex G-200. The enzyme molecule is a tetramer of the alpha 2 beta 2 type. The molecular weights of the alpha- and beta- subunits determined by polyacrylamide gel in the presence of sodium dodecyl sulfate are 52,000--56,000 and 27,000--29,000, respectively. Transketolase contains about two moles of TPP per mole of protein and does not require metal ions for its catalytic activity.
D000818:Animals; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D013835:Thiamine Pyrophosphate; D014174:Transketolase / Q000378:metabolism
D014174:Transketolase; D013835:Thiamine Pyrophosphate
Svoĭstva transketolazy iz pecheni svinyi.