[Effect of the alkylating agent, dipin, on induced hepatocyte proliferation. II. An increase in the DNA synthesis period].
The alkylating drug dipin was injected to mice 2 hours before a partial hepatectomy. The duration of DNA synthesis of proliferating hepatocytes was determined by means of cytophotometry of DNA mass in the nuclei, labeled with 3H-thymidine 30 hours after the operation. The DNA mass was doubled simultaneously in diploid and tetraploid nuclei within 18-26 hours. The DNA in labeled nuclei of the control mice was doubled in 8 hours. The kinetics of 3H-thymidine incorporation at different stages of S-period was similar in alkylated and normal cells.
D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D001388:Aziridines / Q000494:pharmacology*; D001389:Azirines / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D004171:Diploidy; D006498:Hepatectomy; D007399:Interphase / Q000187:drug effects; D007700:Kinetics; D008099:Liver / Q000187:drug effects*; D008115:Liver Regeneration / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010879:Piperazines / Q000494:pharmacology; D011123:Polyploidy; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000477:Alkylating Agents; D001388:Aziridines; D001389:Azirines; D010879:Piperazines; D004247:DNA; D013936:Thymidine
Vliianie alkiliruiushchego agenta dipina na indutsirovannuiu proliferatsiiu gepatotsitov. II. Uvelichenie perioda sinteza DNK.