Current-voltage relationship of the basolateral membrane of a tight epithelium.
The polyene antibiotic nystatin is used to reduce selectively to zero the apical membrane resistance of the rabbit descending colon, allowing the measurement of the current-voltage curve of the basolateral membrane. The I--V relationship is described by the Goldman-Hodgkin-Katz equations allowing calculation of PNa/PK, PCl/PK and PK for the basolateral membrane. Cs+ is found to block inward current (serosa to mucosa) in a manner similar to that found in excitable membranes.
D000818:Animals; D002586:Cesium / Q000494:pharmacology; D003106:Colon / Q000502:physiology*; D007413:Intestinal Mucosa / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects*; D009761:Nystatin / Q000494:pharmacology*; D010539:Permeability; D011817:Rabbits; D012704:Serous Membrane / Q000502:physiology
D009761:Nystatin; D002586:Cesium