Subcellular localization of three degeneration-associated phospholipids in cultured hamster fibroblasts (BHK21 cells).
Subcellular localization of bisphosphatidic acid, semilysobisphosphatidic acid and phosphatidyl-(N-acyl)ethanolamine was studied in normal and degenerating fibroblasts (BHK21 cells) by differential centrifugation. In the normal cells these lipids were highly enriched in the floating fraction consisting mainly of neutral lipid-rich lysosomes. They were also enriched in the mitochondrial fraction. In degenerating cells the high enrichment in the floating fraction was retained, but the other peak was displaced to the crude nuclear fraction. Subfractionation of the crude nuclear fraction indicated that these lipids were not enriched in the purified nuclei. Instead, their concentrations were relatively high in the other subfraction evidently enriched in the large secondary lysosomes characteristic for the degenerating cells. Neither in normal nor degenerating cells were these lipids enriched in the light mitochondrial fraction, where most of the smaller, and probably younger, lysosomes were found. On the basis of these results it is suggested that bisphosphatidic acid, semilysobisphosphatidic acid and phosphatidyl-(N-acyl)ethanolamine are lysosomal in origin. It appears possible that they are specifically associated with the organelles representing the later stages in the lysosomal lifespan.
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D005347:Fibroblasts / Q000032:analysis / Q000648:ultrastructure; D007668:Kidney; D008247:Lysosomes / Q000378:metabolism; D010712:Phosphatidic Acids / Q000032:analysis*; D010714:Phosphatidylethanolamines / Q000032:analysis*; D013347:Subcellular Fractions / Q000032:analysis
D010712:Phosphatidic Acids; D010714:Phosphatidylethanolamines; C021632:bisphosphatidic acid; C021745:semilysobisphosphatidic acid