[Properties of a new, oncornavirus D-associated antigen].
Continuous cells of human origin, HEp-2, and mouse L929 cells taken 24 hours after infection with oncornavirus D were labeled for protein with a mixture of 14C-amino acids and 3H-glucosamine. Specific sera obtained from rabbits were used for testing the virus-specific new antigen in cell extracts and for immunoprecipitation of the new antigen. Electrophoresis in polyacryl amide gel showed the virusspecific new antigen, common for human HEp-2 cells and for oncornavirus-D-infected mouse L929 cells, to be a glycoprotein with a molecular weight of 98,000--100,000 daltons. By its molecular weight it differs from oncornavirus envelope glycoprotein whose molecular weight is 60,000--70,000 daltons. No similar protein was found in noninfected L929 cells.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000302:isolation & purification; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D051379:Mice; D008970:Molecular Weight; D011817:Rabbits; D012190:Retroviridae / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation
D000956:Antigens, Viral; D014764:Viral Proteins
K izucheniiu svoĭstv novogo, assotsiirovannogo s onkornavirusom D antigena.