title: GABA-accumulating neurons in the nucleus raphe dorsalis and periaqueductal gray in the rat: a biochemical and radioautographic study.
abstract: The possibility of a GABAergic innervation of the nucleus raphe dorsalis (NRD) has been investigated by using the following approaches: (i) the identification of the principal neuronal groups afferent to the NDR by using horseradish peroxidase retrograde transport, (ii) the determination of glutamate decarboxylase activity (GAD) in the NRD after lesioning these groups or their putative pathways, and (iii) the radioautographic identification of terminals axons and nerve cells accumulating intraventricularly injected [3H]GABA. The hypothesis of a local GABAergic network is supported by the failure to obtain important changes in GAD after lesions of NRD afferents and the presence in this nucleus of terminals, fibers and nerve cell bodies  accumulating [3H]GABA. It appears that these GABA-accumulating neurons could represent a portion of aperiventricular GABAergic system in the periaqueductal gray and the pontine ventricular gray.
mesh_terms: D000344:Afferent Pathways / Q000201:enzymology; D000818:Animals; D001345:Autoradiography; D001931:Brain Mapping; D001933:Brain Stem / Q000201:enzymology*; D002535:Cerebral Aqueduct / Q000201:enzymology*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006735:Horseradish Peroxidase; D008297:Male; D011149:Pons / Q000201:enzymology; D011903:Raphe Nuclei / Q000201:enzymology*; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D013378:Substantia Nigra / Q000201:enzymology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D012701:Serotonin; D005680:gamma-Aminobutyric Acid; D006735:Horseradish Peroxidase; D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase