Satellite association frequency and number of nucleoli depend on cell cycle duration and NOR-activity. Studies on first, second, and third mitoses of lymphocyte cultures.
In human lymphocyte cultures the frequencies of satellite associations in first, second, and third mitoses were investigated using the BUDR-method. A marked decrease of the association frequency with increasing numbers of cell cycles was found. The number of nucleoli seen in interphase is correlated with the satellite association frequency in the respective metaphase. Satellite association is positively correlated to Ag-staining intensity of the NORs. Individual differences in satellite association are due to differences in NOR activity and in lymphocyte activation. BUDR diminishes somewhat the Ag-staining intensity of the NORs but has no effect on satellite association frequencies. The main reason for the decrease of satellite association frequency in second and third lymphocyte mitoses is presumably a certain dislocation of the original chromosome position during mitosis and a decreased possibility of association during the short interphases. The high association frequency in first mitosis resembles the chromosome position in the long interphase of G0-lymphocytes.
D000328:Adult; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002478:Cells, Cultured; D002842:Chromatids / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure*; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged
D002843:Chromatin; D001973:Bromodeoxyuridine