Sensitivity of cultured rat hepatocytes to aflatoxin B1 and benzo(a)pyrene.
We have tested the sensitivity of a cloned rat hepatocyte line, RL-PR-C, to aflatoxin B1 and benzo(a)pyrene as a function of population-doubling level. The cells were much more sensitive to the cytotoxic action of these agents subsequent to 230 population doublings. This sensitivity corresponded to the enhanced inducibility of aryl hydrocarbon hydroxylase activity by 3-methylcholanthrene.
D000348:Aflatoxins / Q000494:pharmacology*; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis*; D001580:Benzopyrenes / Q000494:pharmacology*; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects* / Q000201:enzymology; D004790:Enzyme Induction; D008099:Liver; D008748:Methylcholanthrene; D051381:Rats
D000348:Aflatoxins; D001580:Benzopyrenes; D008748:Methylcholanthrene; D001189:Aryl Hydrocarbon Hydroxylases