[Lactate dehydrogenase and malate dehydrogenase isoenzymes at different stages of the mitotic cycle in Ehrlich tumor cells].
LDH is represented in the tumor almost exclusively by an extremely slow migrating isozyme LDH-5. There is actually no LDH-1, LDH-2, LDH-3 in the spectrum, while LDH-4 is in small amounts. MDH is represented by cytoplasmic isozyme (S-MDH) and a mitochondrial fraction (m-MDH). The activity of LDH-5 and S-MDH is altered in a synchronous way: it shows a very sharp increase at the very beginning of the mitotic cycle and reaches the maximum value already by the mid-S-phase. The m-MDH activity through the cycle is enhanced twice: at the beginning of the cycle and following the termination of S-phase. There was found a direct correlation between the activity of m-MDH and the rate of anaerobic glycolysis through the mitotic cycle of Ehrlich tumor cells.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D004789:Enzyme Activation; D007527:Isoenzymes / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D051379:Mice; D008938:Mitosis; D013997:Time Factors
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase
Izozimy laktatdegidrogenazy y malatdegidrogenazy na razlichnykh étapakh mitoticheskogo tsikla kletok opukholi Erlikha.