Effects of diamines on ornithine decarboxylase activity in control and virally transformed mouse fibroblasts.
1. The induction of ornithine decarboxylase activity in mouse 3T3 fibroblasts or an SV-40 transformed 3T3 cell line by serum was prevented by addition of the naturally occurring polyamines putrescine (butane-1,4-diamine) and spermidine. Much higher concentrations of these amines were required to fully suppress ornithine decarboxylase activity in the transformed SV-3T3 cells than in the 3T3 fibroblasts. 2. Synthetic alpha omega-diamines with 3--12 carbon atoms also prevented the increase in ornithine decarboxylase activity induced by serum in these cells. The longer chain diamines were somewhat more potent than propane-1,3-diamine in this effect, but the synthetic diamines were less active than putrescine in the 3T3 cells. There was little difference between the responses of 3T3 and SV-3T3 cells to the synthetic diamines propane-1,3-diamine and heptane-1,7-diamine. 3. These results are discussed in relation to the control of polyamine synthesis in mammalian cells.
D000818:Animals; D001769:Blood; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003470:Culture Media; D003959:Diamines / Q000494:pharmacology*; D005347:Fibroblasts / Q000201:enzymology; D051379:Mice; D065108:Ornithine Decarboxylase Inhibitors
D003470:Culture Media; D003959:Diamines; D065108:Ornithine Decarboxylase Inhibitors; D002262:Carboxy-Lyases