A simple procedure for the isolation of a major amelogenin polypeptide component.
A simple, reproducible gel-filtration procedure for the isolation of one of the major 'J-Group' polypeptides of the bovine foetal dental-enamel matrix is described. The purified polypeptide was characterized by amino acid analysis, electrophoresis, cleavage with CNBr and N-terminal analyses. The isolated component is shown to be closely similar to an amelogenin component described by other workers.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D003488:Cyanogen Bromide; D003746:Dental Enamel Proteins / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*
D000596:Amino Acids; D003746:Dental Enamel Proteins; D010455:Peptides; D003488:Cyanogen Bromide