Carbon magnetic resonance studies of the self-aggregation of calf thymus histones.
Self-aggregation of calf thymus histones H2A and H4 was studied by means of 13C magnetic resonance. Analyses of the changes in the intensities of several side-chain resonances, induced by added NaCl, confirm that carbon magnetic resonance can be used to monitor aggregation phenomena more accurately than other physicochemical methods. In particular the validity of an original computer-based method to treat 13C intensities is confirmed. The details of these aggregation phenomena are critically discussed in the light of a simple experiment on a 13C-enriched sample of H2B.
D000818:Animals; D002417:Cattle; D006657:Histones; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011487:Protein Conformation; D013950:Thymus Gland / Q000032:analysis*
D006657:Histones; D046911:Macromolecular Substances