Chromosome breaking activity of human feces and its enhancement by transition metals.
Chloroform-methanol extracts from human fecal samples were found to contain compounds which induce chromosome aberrations in Chinese hamster ovary cells. The induction of chromosome aberrations is stimulated by Cu2+ or Mn2+ and inhibited by Fe2+ or Fe3+. Addition of catalase to the fecal extract or the mixture of fecal extract and Mn2+ reduced the frequency of chromosome aberrations. These properties are indicative of hydroxyradical producing agents.
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002478:Cells, Cultured; D002725:Chloroform; D002869:Chromosome Aberrations; D003300:Copper / Q000494:pharmacology; D006224:Cricetinae; D005243:Feces / Q000032:analysis*; D005260:Female; D006801:Humans; D007501:Iron / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D008677:Metaphase / Q000187:drug effects; D000432:Methanol; D009153:Mutagens / Q000302:isolation & purification; D010053:Ovary / Q000166:cytology
D008670:Metals; D009153:Mutagens; D003300:Copper; D002725:Chloroform; D007501:Iron; D002374:Catalase; D008274:Magnesium; D000432:Methanol