Purification and properties of heat-resistant exotoxin produced by Macrophomina phaseolina (Tassi) Goid in culture.
A partially purified preparation of a water-soluble, heat-resistant, nonspecific exotoxin produced by a strain of Macrophomina phaseolina, isolated from Phaseolus mungo L. could reduce Cu++ ions and produced a red colour with 2,4-dinitrophenyl hydrazine reagent. It caused inhibition of seed germination, wilting of cult seedlings, stunted growth of young seedlings and loss of permeability of the cell membrane. Seedlings of P. mungo, grown in presence of the toxin showed a slight increase in the contents of protein and total RNA over control, but a significant increase in the specific activities of F-1, 6-BP aldolase and G-6-P isomerase.
D003300:Copper; D005658:Fungi / Q000032:analysis; D009183:Mycotoxins / Q000302:isolation & purification / Q000494:pharmacology; D010084:Oxidation-Reduction; D063245:Plant Development; D010944:Plants / Q000187:drug effects
D009183:Mycotoxins; D003300:Copper