The subcellular fractionation of embryonic chick tendon and cartilage cells: a re-examination.
A re-examination of the subcellular fractions obtained from matrix-free chick tendon and cartilage cells has been made since the discovery that three out of four of the micrographs of chick tendon microsomal fractions published in an earlier paper from this laboratory were not authentic. The present studies demonstrate that by using the procedures previously reported it is possible to isolate microsomal and submicrosomal fractions from tendon and cartilage cells which exhibit typical morphology when examined by electron microscopy. These observations are consistent with our original biochemical characterization of subcellular fractions, which we know to be valid. Other publications from this laboratory in which these fractionation procedures have been applied to studies of collagen biosynthesis are in no way compromised, and indeed, most of our data have been confirmed by several other laboratories.
D000818:Animals; D002356:Cartilage / Q000648:ultrastructure*; D002642:Chick Embryo; D013347:Subcellular Fractions / Q000648:ultrastructure; D013710:Tendons / Q000648:ultrastructure*