Occluding-like junctions at mesaxons of central myelin in Anolis carolinensis are not 'tight'. A freeze-fracture-protein tracer analysis.
The junctional complexes of the myelin sheath of central nervous system axons in the American chameleon, Anolis carolinensis, exhibit an intramembrane ridge and groove construction in freeze-fracture replicas that has usually been interpreted in other organisms as evidence for an occluding or tight intercellular junction. Close examination of PF fracture face ridges, however, shows them to be made up of discontinuous rows of particles of variable length separated by frequent gaps of non-uniform width. Introduction of horseradish peroxidase into the intercellular milieu of the lizard central nervous system is followed by appearance of this protein in interlamellar spaces of the myelin sheath and in the intercellular spaces containing focal membrane fusions that correspond precisely in position and center-to-center spacing to the ridges and grooves in platinum replicas of the same tissue. Since the junctional ridges on PF fracture faces in these mesaxonal junctional complexes are conspicuously discontinuous and since the areas within the myelin sheath where these junctional complexes are located inner and outer mesaxons) are readily permeated by exogenous protein tracer, it is concluded that the junctional complexes of central myelin mesaxons, heretofore incorrectly interpreted as functionally tight, are actually very leaky and probably contribute only to the structural stability of the myelin sheath architecture.
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure*; D005614:Freeze Fracturing; D006735:Horseradish Peroxidase / Q000378:metabolism; D007365:Intercellular Junctions / Q000378:metabolism / Q000648:ultrastructure*; D008116:Lizards / Q000033:anatomy & histology*; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000378:metabolism / Q000648:ultrastructure*; D012583:Schwann Cells / Q000378:metabolism / Q000648:ultrastructure*
D006735:Horseradish Peroxidase