Utilization of liposomes for correction of the metabolic and bactericidal deficiencies in chronic granulomatous disease.
IgG-coated liposomes containing glucose oxidase (GO) in order to provide a means of generating H2O2 were prepared. Leukocytes from patients with chronic granulomatous disease (CGD) which are lacking a means of generating H2O2, ingested the IgG-coated liposomes and metabolic oxidative deficiencies of glucose-1-14C oxidation and iodination were normalized. Both of these activities have been shown to depend upon the availability of H2O2 within polymorphonuclear leukocytes. Improvement in the capacity to kill Staphylococcus aureus by chronic granulomatous disease leukocytes were observed under similar conditions. Thus, it is possible to restore the oxidative metabolic capacities to CGD leukocytes by the introduction of glucose oxidase containing liposomes to these cells.
D000328:Adult; D005949:Glucose Oxidase / Q000378:metabolism / Q000627:therapeutic use; D006105:Granulomatous Disease, Chronic / Q000188:drug therapy* / Q000201:enzymology / Q000276:immunology; D006600:Hexosephosphates / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000008:administration & dosage; D007962:Leukocytes / Q000201:enzymology / Q000276:immunology; D008081:Liposomes / Q000627:therapeutic use*; D010545:Peroxides / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects; D013211:Staphylococcus aureus / Q000276:immunology
D006600:Hexosephosphates; D007074:Immunoglobulin G; D008081:Liposomes; D010545:Peroxides; D005949:Glucose Oxidase