Colorimetric determination of boric acid in prawns, shrimp, and salted jelly fish by chelate extraction with 2-ethyl-1,3-hexanediol.
Borate was directly chelate-extracted from foods with 5% 2-ethyl-1,3-hexanediol (EHD) in n-hexane-n-butyl acetate mixture (8 + 2), from which borate was selectively transferred into 1% NaOH, since EHD-chelated boron did not react with curcumin to develop color. Finally, an aliquot of the alkaline solution was acidified with HCl and reacted with curcumin in a rotary evaporator. Color development was increased by heating for 8 min at 80 degrees C under reduced pressure of 16 mm Hg. Frozen shrimp and prawns (peeled and with shells) and salted jelly fish were analyzed by the proposed method. Results were compared with the contemporary official method of Japan based on curcumin reaction on an incinerated sample. Over 90% of the boric acid was recovered by the proposed method when samples were fortified with 20 ppm boric acid. Recoveries were superior to those of the official method especially for shrimp and prawns with shells and salted jelly fish. Detection limit of boric acid is 1 ppm. Moreover, the method requires only about 1 hr for analysis of one sample, making it suitable for routine analysis.
D001888:Boric Acids / Q000302:isolation & purification*; D002614:Chelating Agents; D003124:Colorimetry; D033364:Decapoda / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D005520:Food Preservatives / Q000032:analysis*; D000441:Hexanols; D006358:Hot Temperature; D037401:Scyphozoa / Q000032:analysis; D012758:Shellfish / Q000032:analysis*
D001888:Boric Acids; D002614:Chelating Agents; D005520:Food Preservatives; D000441:Hexanols