title: Protein synthesis in isolated human skeletal muscle tissue: evaluation of an experimental model.
abstract: Amino acids were incorporated into soluble proteins, myosin and myoglobin at constant rates for at least 4 h on incubation of isolated human skeletal muscle fibres. The time course of incorporation of leucine into proteins not attached to ribosomes was approximately constant, suggesting a continuous termination of proteins. A comparison between estimated pool size of ribosomes and incorporation rate of leucine into proteins indicated that initiation of peptides occurred even in the absence of insulin.
mesh_terms: D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D001842:Bone and Bones / Q000378:metabolism*; D003080:Cold Temperature; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D008954:Models, Biological; D009132:Muscles / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D014176:Protein Biosynthesis; D011691:Puromycin / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D014443:Tyrosine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
chemical_list: D007328:Insulin; D014443:Tyrosine; D001312:Aurintricarboxylic Acid; D010649:Phenylalanine; D011691:Puromycin; D012313:RNA; D007930:Leucine; D014529:Uridine