Inhibitory effect of the antioxidant butylated hydroxyanisole on the activation of the carcinogen benzo(a)pyrene.
The effect of the antioxidant butylated hydroxyanisole (BHA) on benzo(a)pyrene (BP) metabolites binding to DNA, percentage of diols and phenols in the total amount of BP metabolites extracted to organic solvent, were studied in : incubation mixture of rat liver microsomes, cultured mouse embryo cells, human skin, and human lymphocytes. The amount of BP metabolites bound to DNA in mouse embryo cells and human skin decreased in the presence of BHA. No effect was found in human lymphocytes. Percent of phenols increased in mouse embryo cell cultures; percent of diols decreased in cultures of human skin and lymphocytes.
D000818:Animals; D000840:Anisoles / Q000494:pharmacology*; D001580:Benzopyrenes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D001711:Biotransformation / Q000187:drug effects; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D012867:Skin
D000840:Anisoles; D001580:Benzopyrenes; D002083:Butylated Hydroxyanisole; D004247:DNA