title: Extracellular fluid of the kidney preserved by the Collins technique.
abstract: Hilar drainage fluid of dog kidneys was analyzed as an approximation to renal extracellular fluid after preservation by flushing with chilled high K-low Na solution (Collins C4) followed by ice-cold storage for 24 and 48 hr in a bath of flushing medium. Compared with the medium, Na and Cl were increased to 30 mM/liter and K decreased slightly to 93 mM/liter. Glucose decreased, whereas lactate, lactic dehydrogenase, and creatine phosphokinase increased by significant amounts in both the drainage fluid and bath. The inulin space of the undrained kidney average 37% of wet weight. Calculated intracellular Na and Cl concentrations averaged 50 and 37 mM/kg cell water while K remained within normal limits. A significant fraction of red blood cells retained during initial flushing entered the effluent during storage. Bath and effluent composition of a human cadaver kidney approximated those of a dog.
mesh_terms: D000818:Animals; D004285:Dogs; D004322:Drainage; D005110:Extracellular Space / Q000032:analysis*; D006801:Humans; D007444:Inulin / Q000378:metabolism; D007668:Kidney / Q000033:anatomy & histology / Q000378:metabolism*; D009926:Organ Preservation; D009929:Organ Size; D013997:Time Factors; D014021:Tissue Preservation
chemical_list: D007444:Inulin