Histochemical demonstration of mouse submandibular esterproteases with a new chromogenic substrate.
A new method for the histochemical demonstration of mouse submandibular esterproteases has been developed. The substrate is N-acetyl-L-methionine alpha-naphthyl ester. The main enzyme reaction was found in the apical region of the secretory tubules with a marked sex difference as expected. First attempts were made to differentiate histochemically between the various esterproteolytic enzymes.
D000818:Animals; D004950:Esterases / Q000032:analysis*; D005260:Female; D006651:Histocytochemistry; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives; D051379:Mice; D009280:Naphthaleneacetic Acids; D012737:Sex Factors; D013363:Submandibular Gland / Q000201:enzymology*; D013379:Substrate Specificity
D009280:Naphthaleneacetic Acids; C020854:N-acetylmethionine alpha-naphthyl ester; D008715:Methionine; D004950:Esterases