title: [Product of second-stage riboflavin biosynthesis in Pichia guilliermondii].
abstract: Dialyzed extracts of the mutants of Pichia guilliermondii RG80 (rib3, his-) and RA39 (rib3, ade-) in which the third step of flavinogenesis was genetically blocked converted GTP into 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate and 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine in the presence of NADPH2. Apparently, 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate is a true precursor of riboflavin whereas 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine is formed upon its hydrolysis.
mesh_terms: D000631:Aminopyridines / Q000096:biosynthesis; D001203:Ascomycota / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D009154:Mutation; D010843:Pichia / Q000378:metabolism*; D012256:Riboflavin / Q000096:biosynthesis*; D012267:Ribosemonophosphates / Q000096:biosynthesis
chemical_list: D000631:Aminopyridines; D012267:Ribosemonophosphates; D006160:Guanosine Triphosphate; D012256:Riboflavin
vernacular_title: O produkte vtorogo étapa biosinteza riboflavina u Pichia guilliermondii.