Disparate Na+-requirement of taurocholate and indocyanine green uptake by isolated hepatocytes.
Uptake of both taurocholate and indocyanine green (ICG) by isolated rat hepatocytes was saturable and obeyed Michaelis-Menten kinetics. Only uptake of taurocholate exhibited Na+-dependence, a phenomenon compatible with active membrane transport. These findings suggest that the mechanisms responsible for hepatocellular uptake of bile acids and anionic dyes differ fundamentally.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D066298:In Vitro Techniques; D007208:Indocyanine Green / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D012964:Sodium / Q000494:pharmacology*; D013656:Taurocholic Acid / Q000378:metabolism*
D013656:Taurocholic Acid; D012964:Sodium; D007208:Indocyanine Green