title: Cardiac lymph flow in conscious dogs.
abstract: The cardiac lymphatic duct was cannulated in dogs and the exteriorized cannula allowed chronic collection of lymph during the awake state for as long as 3 wk. The surgical methodology and inherent difficulties in the technique are describ:d. Cardiac lymph flow ranged from 0.45--5.6 ml/h in the control state in 14 dogs. An occluding device and flow probe were placed on the circumflex coronary artery (CFX); ultrasonic segment length crystals were placed in the left ventricular free wall in 4 dogs. Occlusion of the CFX in these conscious dogs caused lymph flow to fall as great as 46% below control during the 1st half-hour. Reperfusion of the occluded vessel caused an increase in lymph flow as great as 67% above control. The effect on cardiac lymph flow was demonstrated for a few select drugs that have known effects on the cardiovascular system. Cardiac lymph flow was altered from control as follows: isoproterenol, 42 +/- 11% increase; RO 2-2985, 118 +/- 8% increase; verapamil, 101 +/- 10% increase; propranolol caused no significant change. The conscious dog with cardiac lymph vessel cannulated should provide a model to further study the complexities of cardiac metabolism and physiology without interference of anesthesia and surgical stress.
mesh_terms: D000818:Animals; D004285:Dogs; D006321:Heart / Q000033:anatomy & histology / Q000502:physiology*; D007545:Isoproterenol / Q000494:pharmacology; D007832:Lasalocid / Q000494:pharmacology; D008196:Lymph / Q000502:physiology*; D008208:Lymphatic System / Q000033:anatomy & histology; D009200:Myocardial Contraction / Q000187:drug effects; D011433:Propranolol / Q000494:pharmacology; D012212:Rheology; D014700:Verapamil / Q000494:pharmacology
chemical_list: D011433:Propranolol; D014700:Verapamil; D007545:Isoproterenol; D007832:Lasalocid