Purification of an Endogenous polynucleotide phosphorylase from Brevibacterium JM98A.
Polynucleotide phosphorylase was purified form Brevibacterium JM98A (ATCC 29895). Homopolynucleotides were arsenolysed in the order polyadenylate greater than polycytidylic acid greater than polyuridylic acid greater than polyguanylate. The products were ribonucleoside 5'-monophosphates.
D000244:Adenosine Diphosphate / Q000378:metabolism; D001951:Brevibacterium / Q000201:enzymology*; D002474:Cell-Free System; D011119:Polynucleotides / Q000378:metabolism; D011117:Polyribonucleotide Nucleotidyltransferase / Q000302:isolation & purification* / Q000378:metabolism
D011119:Polynucleotides; D000244:Adenosine Diphosphate; D011117:Polyribonucleotide Nucleotidyltransferase