Further studies on partially purified calf thymus DNA polymerase a.
Attempts to prevent the urea conversion of a 200-230,000 molecular weight DNA polymerase alpha to a 150-170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50-70,000 fragment or subunit from the DNA polymerase subunit. Addition of this 50-70,000 subunit to the polymerase subunit does not aid the binding of the enzyme to DNA, but does have an effect on the utilisation of synthetic template-initiator complexes by the polymerase subunit.
D000818:Animals; D002417:Cattle; D004257:DNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D013329:Structure-Activity Relationship; D013698:Templates, Genetic; D013950:Thymus Gland / Q000201:enzymology*
D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase