Pattern of segregation of chicken HPRT phenotype in Chinese hamster-chick red blood cell hybrids.
The pattern of segregation of hypoxanthine phosphoribosyltransferase (HPRT, E.C. 2.4.2.8) was determined in synchronized Chinese hamster-chick red blood cell hybrids. Three hybrid lines were synchronized at the G1-S boundary. Bromodeoxyuridine pulses were subsequently applied throughout the S phase, and the frequency of the segregant clones was determined. It was found that the segregation of the chicken-specific HPRT phenotype associated with the loss of a chromosome was potentiated by bromodeoxyuridine administered during the first hour following release of the block.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae / Q000235:genetics*; D003412:Cricetulus / Q000235:genetics*; D003470:Culture Media; D004912:Erythrocytes / Q000201:enzymology*; D006822:Hybrid Cells / Q000201:enzymology*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism
D003470:Culture Media; D007041:Hypoxanthine Phosphoribosyltransferase; D001973:Bromodeoxyuridine