Preparation and properties of amphipathic enzyme-polymer conjugates.
Amphipathic enzyme-polymer conjugates were prepared by reaction of hydrophilic enzymes with an anhydride polymer partially substituted with pendant hydrophobic groups. The products formed non-covalent aggregates of high molecular weight, dissociable by non-ionic detergents and urea and subject to additional aggregation at high ionic strength. Oil-in-water emulsions formed in the presence of such conjugates were shown to possess enzyme activity associated with the surface of the water-immiscible liquid. Fluorochrome labelling of conjugates showed that binding occurred at the surface of solvent droplets, and the conjugates were also found to aggregate liposomes.
D000581:Amidohydrolases; D003902:Detergents; D004800:Enzymes, Immobilized; D008081:Liposomes; D046911:Macromolecular Substances; D009994:Osmolar Concentration; D010399:Penicillin Amidase; D011108:Polymers / Q000138:chemical synthesis; D013499:Surface Properties; D014357:Trypsin; D014508:Urea
D003902:Detergents; D004800:Enzymes, Immobilized; D008081:Liposomes; D046911:Macromolecular Substances; D011108:Polymers; D014508:Urea; D014357:Trypsin; D000581:Amidohydrolases; D010399:Penicillin Amidase