The effect of various potential inhibitors, activators and inducers on the N-oxidation of 3-substituted pyridines in vitro.
1. The N-oxidation of pyridine, 3-methylpyridine and 3-chloropyridine was inhibited by SKF525A and DPEA. The C-oxidation of 3-methylpyridine was also inhibited by these compounds. 2. The N-oxidation of these pyridines was also inhibited by various other nitrogenous substrates including n-octylamine. 3. Incubation in an atmosphere of carbon monoxide resulted in inhibition of both C- and N-oxidation of 3-methylpyridine. 4. Any treatment of microsomes which resulted in a reduction of cytochrome P-450 also produced a concomitant fall in N-oxidation of the pyridines. 5. Pretreatment of animals with phenobarbitone resulted in an increase in the N-oxidation of the pyridines. Pretreatment with 3-methylcholanthrene had no appreciable effect on the N-oxidation of the pyridines in vitro.
D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology; D006224:Cricetinae; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D010634:Phenobarbital / Q000494:pharmacology; D011725:Pyridines / Q000378:metabolism*; D011817:Rabbits
D004791:Enzyme Inhibitors; D011725:Pyridines; D008748:Methylcholanthrene; D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital