[Amination and biosynthesis of glutamate by R- and S-forms of Bacillus thuringiensis].
These studies were designed to determine the biochemical nature of the Bacillus thuringiensis growth being dependent on glutamate during cultivation in a minimal medium. This is possible to be due to the absence of enzymes which catalyze glutamic acid synthesis by direct amination of alpha-ketoglutaric acid, glutamate dehydrogenase and glutamate synthase, and a decrease in the activity of the enzyme catalyzing amination of pyruvic acid, alanine dehydrogenase. It has been shown that the lack of glutamate can be compensated by histidine and proline; in this case, the growth efficiency of R form is greater than that of S form which is consistent with an increased rate of protein synthesis of R form.
D000409:Alanine / Q000378:metabolism; D000410:Alanine Transaminase / Q000378:metabolism; D000586:Amination; D001413:Bacillus thuringiensis / Q000201:enzymology / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005970:Glutamate Synthase / Q000378:metabolism; D005971:Glutamates / Q000096:biosynthesis / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism
D005971:Glutamates; D006639:Histidine; D010088:Oxidoreductases; D005970:Glutamate Synthase; D005969:Glutamate Dehydrogenase; D000410:Alanine Transaminase; D000409:Alanine
Aminirovanie i biosintez glutamata u R- i S-form Bacillus thuringiensis.