title: Separation of the alpha chains of type I and III collagens by SDS-polyacrylamide gel electrophoresis.
abstract: A method for the separation of type III collagen from type I collagen by SDS-polyacrylamide gel electrophoresis has been developed. This is based on the observation that the presence of 3-4 M urea decreases the mobility of the alpha 1 [III] chain to a greater extent than those of the alpha 1[I] and alpha 2 chains, although the alpha 1[I] and alpha 1[III] chains move at the same rate in the absence of urea. An attempt to separate the alpha 1[II] chain of type II collagen from the alpha 1[I] chain was unsuccessful under the experimental conditions employed.
mesh_terms: D000818:Animals; D002417:Cattle; D003094:Collagen / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D010455:Peptides / Q000302:isolation & purification; D012867:Skin / Q000032:analysis
chemical_list: D046911:Macromolecular Substances; D010455:Peptides; D003094:Collagen