The effect of glucose on the synthesis of prostaglandins by the renal papilla of the rat in vitro.
Renal pappillae from rats were incubated in vitro. The release of prostaglandin by this tissue was found to be inversely related to the glucose concentration of the buffer. Estimates of prostaglandin release were determined by a rat stomach strip bioassay, and in some instances, gas-chromatography and mass spectrometry. When incubated in the presence of C14-arachidonic acid, the specific activity of prostaglandins E2 and F2 alpha released by the tissue was lower at the lower glucose concentration. Provision of 625 micrograms/ml of exogenous arachidonic acid in the buffer obliterated the effect of glucose on prostalglandin release. These data indicate that increasing amounts of glucose suppresses prostaglandin synthesis in the renal papilla of the rat, and that the mechanism of this phenomenon is related to the release of arachidonic acid from its storage pools in tissue lipids.
D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D001681:Biological Assay; D002478:Cells, Cultured; D002849:Chromatography, Gas; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology*; D007679:Kidney Medulla / Q000378:metabolism*; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D013058:Mass Spectrometry; D009994:Osmolar Concentration; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D051381:Rats
D001095:Arachidonic Acids; D003470:Culture Media; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D008353:Mannitol; D005947:Glucose