Improved method for obtaining streptomycin-dependent mutants from Pasteurella multocida and Pasteurella haemolytica, using N-methyl-N'-nitro-N-nitrosoguanidine.
N-Methyl-N'-nitro-N-nitrosoguanidine was used in a modified and improved procedure to obtain streptomycin-dependent mutants of type A Pasteurella multocida and type 1 Pasteurella haemolytica. The relative virulence of these mutants was determined by mouse inoculation with and without streptomycin.
D000818:Animals; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D051379:Mice; D009154:Mutation / Q000187:drug effects*; D010325:Pasteurella / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D013307:Streptomycin / Q000494:pharmacology*; D013696:Temperature; D014774:Virulence
D008769:Methylnitronitrosoguanidine; D013307:Streptomycin