Identification of the translation products of human fibroblast interferon mRNA in reticulocyte lysates.
Messenger RNA was purified from human foreskin fibroblasts FS11, a high interferon-producer line, after induction with synthetic double-stranded RNA. The mRNA was translated in a cell-free protein-synthesis system from rabbit reticulocytes. The translation products, containing biologically active human interferon, were immunoprecipitated by a serum from rabbits immunized against partially purified interferon. Analysis of the immunoprecipitate by polyacrylamide gel electrophoresis in dodecylsulfate shows that the product of human fibroblast interferon mRNA is a 23000-Mr polypeptide. Methods are described for the synthesis and rapid identification of this polypeptide, which should be useful for structural analysis of interferon and isolation of its mRNA.
D000818:Animals; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D007372:Interferons / Q000096:biosynthesis*; D008297:Male; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012156:Reticulocytes / Q000378:metabolism*; D012867:Skin / Q000378:metabolism
D012333:RNA, Messenger; D007372:Interferons