[Application of sucrose density gradient in disk electrophoresis of proteins].
The methods are suggested when either fluid with the sucrose density gradient or the combination of sucrose with polyacrylamide gel are used as a zonal carrier in disk electrophoresis. Possibility is shown and conditions are selected for concentration and subsequent separation of proteins in the fluid with the sucrose density gradient in disk electrophoresis as narrow zones with clear boundaries. By the methods proposed the sarcoplasmic proteins of the rat myocardium and incorporated creatine kinase isoenzymes were separated.
D000818:Animals; D003402:Creatine Kinase / Q000032:analysis*; D004589:Electrophoresis, Disc / Q000379:methods; D007527:Isoenzymes / Q000032:analysis*; D009124:Muscle Proteins / Q000032:analysis*; D009206:Myocardium / Q000032:analysis / Q000201:enzymology; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000032:analysis; D013395:Sucrose
D007527:Isoenzymes; D009124:Muscle Proteins; D013395:Sucrose; D003402:Creatine Kinase
Primenenie gradienta plotnosti sakharozy pri disk-elektroforeze belkov.