title: Short chain fatty acids in plasma and brain: quantitative determination by gas chromatography.
abstract: A reliable and practicable method for the determination of short chain fatty acids in plasma and brain tissue is presented. The sample preparation by partition chromatography on silicic acid allows subsequently a quantitation of short chain fatty acids without interference by methylmalonic acid, or other more polar compounds. With the gas-chromatographic system 2-methylbutyrate is separated from isovalerate. Reference values are given for plasma. The system is also useful in combination with mass spectrometry.
mesh_terms: D000328:Adult; D001923:Brain Chemistry; D002087:Butyrates / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods*; D005069:Evaluation Studies as Topic; D005227:Fatty Acids / Q000097:blood* / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D008764:Methylmalonic Acid / Q000097:blood; D008875:Middle Aged; D011422:Propionates / Q000097:blood; D014631:Valerates / Q000097:blood
chemical_list: D002087:Butyrates; D005227:Fatty Acids; D011422:Propionates; D014631:Valerates; D008764:Methylmalonic Acid