Early influx of horseradish peroxidase into axons of the hypoglossal nerve during Wallerian degeneration.
Horseradish peroxidase (HRP) was applied around mouse hypoglossal nerves which were damaged by a crush or a ligature. HRP was then visualized distal to the lesions by light- and electron microscopic histochemistry. At the injury the enzyme entered axons and could also be detected several millimetres down in the distal segment. By 24 h reaction product (r.p.) was either diffusely distributed in the axoplasm or present in various vesicular organelles. Our results indicate that there is a rapid influx of macromolecules into axons after a lesion to a nerve. A similar uptake of 'wound substances' into axons distal to an injury might well have some relation to the process by which axonal breakdown is initiated during Wallerian degeneration.
D000042:Absorption; D000818:Animals; D002118:Calcium / Q000378:metabolism; D006651:Histocytochemistry; D006735:Horseradish Peroxidase / Q000378:metabolism; D007002:Hypoglossal Nerve / Q000378:metabolism / Q000473:pathology; D061228:Hypoglossal Nerve Injuries; D051379:Mice; D008854:Microscopy, Electron; D009410:Nerve Degeneration; D014855:Wallerian Degeneration
D006735:Horseradish Peroxidase; D002118:Calcium