Spectrophotometric and cytochemical studies on azures A, B and C.
The cytochemical use of azures A, B and C, propared with either N HCL and potassium metabisulphite or with sodium hydrosulphite in tissue sections were investigated. Both in situ absorption curves of nuclei stained with each of these dye-SO2 reagents as well as in vitro absorption data of acqueous solutions of the dyes are also presented. It has been pointed out that the mechanism of staining with azure A-SO2 and azure C Eosinate-SO2 is the same as that of the conventional Feulgen reaction with Schiff reagent but that of staining with azure B-SO2 is by the modified Feulgen reaction because this dye does not contain any primary amino group.
D000818:Animals; D001399:Azure Stains; D004247:DNA / Q000032:analysis; D006651:Histocytochemistry / Q000379:methods*; D008099:Liver / Q000032:analysis / Q000166:cytology; D010640:Phenothiazines; D051381:Rats; D013057:Spectrum Analysis / Q000379:methods*
D001399:Azure Stains; D010640:Phenothiazines; D004247:DNA