Metabolism of prostaglandin E1 and of glutathione conjugate of prostaglandin A1 (GSH-prostaglandin A1) by prostaglandin 9-ketoreductase from rabbit kidney.
Rabbit kidney prostaglandin 9-ketoreductase was found to metabolize the glutathione conjugate of prostaglandin A1 (GSH-prostaglandin A1). Apparent Km (GSH-prostaglandin A1) 13 microM and apparent Km (prostaglandin E1) 200 microM. The cytosolic preparation was subjected to gelfiltration and isoelectric focusing, which revealed that metabolism of prostaglandin E1 and GSH-prostaglandin A1 occurs by means of the same fractions. Furthermore, prostaglandin E1 and GSH-prostaglandin A1 are competitive inhibitors of the enzyme, when GSH-prostaglandin A1 and prostaglandin E1 are tested as substrates, respectively. It si concluded, that GSH-prostaglandin A1 is a much better substrate for prostaglandin 9-ketoreductase from rabbit kidney than is prostaglandin E1.
D000818:Animals; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008297:Male; D011454:Prostaglandins A / Q000378:metabolism*; D011455:Prostaglandins A, Synthetic; D011458:Prostaglandins E / Q000378:metabolism*; D011817:Rabbits
D011454:Prostaglandins A; D011455:Prostaglandins A, Synthetic; D011458:Prostaglandins E; C021031:GSH-prostaglandin A1; D006911:Hydroxyprostaglandin Dehydrogenases; D005978:Glutathione