The use of dipeptide-p-nitranilides for the study of aminopeptidase specificity.
The use of dipeptide-p-nitranilides for the study of 2 placental aminopeptidases separated on Sephadex G200 helped in establishing some regular features of their specifities. The high-molecular (320,000 daltons) one prefers Phe in position P'1 to Leu, whereas the lower-molecular aminopeptidase (145,000 daltons) prefers Leu. The high-molecular aminopeptidase splits very slowly the N-terminal Leu when Gly is in adjacent position. Leu-Gly-p-NA is therefore an inhibitor of this AP.
D000626:Aminopeptidases / Q000378:metabolism*; D000813:Anilides; D004151:Dipeptides; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013379:Substrate Specificity
D000813:Anilides; D004151:Dipeptides; D007527:Isoenzymes; D000626:Aminopeptidases