[Glycosylation and isoelectric properties of complete and defective rabies viruses (author's transl)].
From a structural point of view an essential distinction between complete and defective rabies viruses is difference in size. In addition, isoelectric properties differ. The complete virus has an isoelectric point approaching neutrality, whereas the defective virus focuses between pH 3-4.7. The isoelectric points of the glycoprotein from complete and defective viruses differ in a corresponding fashion. The Pasteur virus cultivated on BHK21C13 cells, contains glycoproteins, the glycopeptides of which have a structure containing the following five monosaccharides: sialic acid, D-galactose, (N-acetyl)D-glucosamine, D-mannose and L-fucose. The glycosylation of the glycoproteins is different, at least in so far as the relative sialic acid/glucosamine ratio is concerned.
D000117:Acetylglucosamine / Q000032:analysis; D002478:Cells, Cultured; D003673:Defective Viruses / Q000378:metabolism*; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism*; D007526:Isoelectric Point; D008358:Mannose / Q000032:analysis; D011820:Rabies virus / Q000378:metabolism*; D012794:Sialic Acids / Q000032:analysis
D006023:Glycoproteins; D012794:Sialic Acids; D005643:Fucose; D008358:Mannose; D000117:Acetylglucosamine; D005690:Galactose
Glycosylation et propriétés isoélectriques des virus rabiques complets et défectifs.