[Application of immobilized animal liver microsomal fraction to the reaction of 1,4-benzdiazepine-2-one conversion].
The albino rat strain Wistar and Shynashylla rabbit liver microsomal fraction was immobilized by incorporation into polyacrylamide gel. It is shown, that the microsomal fraction immobilized by such a method catalyzes the hydrolysis of 1,4-benzdiazepine-2 ones, 3-acetoxyderivatives, hydroxylation in position 3 of 1,4-benzdiazepine-2-ones and reduction of nitrazepame. The storage thermostability and stability of the immobilized preparations were studied. Immobilization of the animal liver microsomal fraction was conducted on sylochrome by cyanuric chloride and 2,4-toluylenediisocyanate.
D000818:Animals; D001570:Benzodiazepinones / Q000378:metabolism*; D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism*; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D011817:Rabbits; D013696:Temperature
D001570:Benzodiazepinones; D004800:Enzymes, Immobilized; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D006867:Hydrolases
Ispol'zovanie immobilizovannoĭ fraktsii mikrosom pecheni zhivotiykh dlia reaktsiĭ prevrascheniia 1,4-benzdiazepin-2-onov.