Binding of IgG and complement protein by monosodium urate monohydrate and other crystals.
IgG adsorbed to negatively charged crystals of monosodium urate monohydrate was restricted to cationic species of immunoglobulin. Similar binding of smaller amounts of IgG was observed with crystals other than monosodium urate monohydrate. Heated monosodium urate monohydrate and amorphous sodium urate adsorbed greater amounts of IgG and other proteins. Complement activation as measured by electrophoretic conversion of C3, was not related to the amount of IgG adsorbed by the crystals studied. C1q was also adsorbed to some degree by all crystals studied but did not correlate with complement activation.
D003167:Complement Activation; D003176:Complement C3 / Q000378:metabolism*; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D014527:Uric Acid / Q000378:metabolism*
D003176:Complement C3; D007074:Immunoglobulin G; D014527:Uric Acid; D012964:Sodium; D011188:Potassium