Hydrophobic esters of cellulose: properties and applications in biochemical technology.
We have investigated the utility for enzyme immobilization of several hydrophobic cellulose esters, as a function of solvent composition, extent of esterification, and enzyme. Phenoxyacetyl cellulose was also used for immobilization of rat liver microsomes, hydrophobic chromatography of proteins, and removal of Triton X-100 from protein solutions. Phenoxyacetyl groups esterified to cellulose were much less subject to enzymatic hydrolysis than soluble phenoxyacetyl esters.
D001665:Binding Sites; D001669:Biochemical Phenomena; D001671:Biochemistry; D002482:Cellulose; D002845:Chromatography / Q000379:methods; D004800:Enzymes, Immobilized; D004952:Esters; D008861:Microsomes / Q000201:enzymology; D010642:Phenoxyacetates; D013672:Technology
D004800:Enzymes, Immobilized; D004952:Esters; D010642:Phenoxyacetates; D002482:Cellulose