title: Glycosylation of mouse embryonic hemoglobins in cultured yolk sac erythroblasts.
abstract: Embryonic hemoglobins of yolk sac nucleated erythroblasts were glycosylated in vitro by (3H)-glucose and the rate of glycosylation was maximal within 24 h of incubation. Isoelectric focusing of the glycosylated products revealed that both EI and EII embryonic hemoglobins have glycosylated components, EIg, EIIg, with pI very close to the native hemoglobins.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004900:Erythroblasts / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005865:Gestational Age; D005947:Glucose / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D007525:Isoelectric Focusing; D051379:Mice; D013997:Time Factors; D015017:Yolk Sac / Q000378:metabolism*
chemical_list: D006454:Hemoglobins; D005947:Glucose