Preparation of cytosol for steroid receptor determinations with the Beckman "Airfuge".
Preparation of cytosols from several estrogen target organs was compared, with use of the Beckman Airfuge and a preparative ultracentrifuge. The specific estrogen-binding capacities of cytosols prepared with these instruments were indistinguishable. Similarly, the sedimentation profiles of estrogen-receptor species as determined by sucrose gradient centrifugation were identical. We conclude that the Airfuge provides a rapid, accurate, and inexpensive means of preparing cytosols for measurements of steroid receptors in the clinical laboratory.
D000818:Animals; D001943:Breast Neoplasms / Q000032:analysis*; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D003600:Cytosol / Q000032:analysis* / Q000648:ultrastructure; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000032:analysis*; D011247:Pregnancy; D051381:Rats; D011960:Receptors, Estrogen / Q000032:analysis* / Q000378:metabolism
D011960:Receptors, Estrogen; D004958:Estradiol