Synthesis of procollagen by odontogenic cells of rabbit tooth germ.
Studies were performed to determine whether cultured odontogenic cells from rabbit tooth germ (RP cell) could synthesize dentine-like collagen. When cells were cultured with [14C]proline, 33% of the total incorporated proteins present were collagenous. Cultured RP cells were labeled with [14C]proline in the presence of beta-aminopropionitrile. The resulting fractions, on analysis by CM-cellulose chromatography, contained three radioactive protein peaks, alpha 1(I), [alpha 1(III)]3, alpha 2. From the radioactive measurements, RP cells synthesized a significant amount of type III collagen, comparable to type I collagen. DEAE-cellulose chromatography was used to separate collagen molecules from collagen precursors. The results showed that 60% of total collagen precursor was type III precursor and the remainder was type I precursor. CM-cellulose chromatography of CNBr peptides of collagen from culture medium and cell extract revealed the presence of type I and type III collagen. Thus, the RP cell, which is a diploid cell, is unique in the predominance of type III collagen in culture, differing thereby from the character of collagen in vivo.
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000302:isolation & purification; D007180:Incisor; D008970:Molecular Weight; D009805:Odontogenesis; D010446:Peptide Fragments / Q000032:analysis; D011347:Procollagen / Q000096:biosynthesis*; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D014083:Tooth Germ / Q000378:metabolism*
D010446:Peptide Fragments; D011347:Procollagen; D003094:Collagen; D011392:Proline