title: An experimental assessment of macroscopic enzyme techniques for the autopsy demonstration of myocardial infarction.
abstract: Experimental cardiac infarction produced in dogs by coronary artery ligation has been used to investigate the value of macroscopic histochemical enzyme loss in the diagnosis of myocardial infarction at post-mortem. Creative phosphokinase and non-specific dehydrogenase methods gave the best results but became positive only 5-6 hr after infarction. It is concluded that the method is of limited value in autopsy practice.
mesh_terms: D000818:Animals; D001329:Autolysis / Q000201:enzymology; D001344:Autopsy; D004796:Clinical Enzyme Tests; D003402:Creatine Kinase / Q000378:metabolism; D004285:Dogs; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000201:enzymology; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D009580:Nitroblue Tetrazolium; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D003402:Creatine Kinase