Crystallization and preliminary crystallographic data of chicken gizzard G-actin . DNase I complex and Physarum G-actin . DNase I complex.
Smooth muscle G-actin from chicken gizzard and Physarum plasmodium G-actin both interact with DNase I and form 1 : 1 complexes. These complexes were crystallized by using polyethylene glycol 6000 as a precipitant. Both crystals belong to the same orthorhombic space group P2(1)2(1)2(1). The cell dimensions of chicken gizzard G-actin.DNase I complex are a=42.00 +/- 0.07 A, b=225.3 +/- 0.4 A, and c=77.4 +/- 0.1 A, while those of Physarum G-actin.DNase I complex are a=42 A, b=221 A, and c=77 A.
D000199:Actins / Q000302:isolation & purification; D000818:Animals; D002645:Chickens; D003460:Crystallization; D003851:Deoxyribonucleases / Q000302:isolation & purification; D005895:Gizzard, Avian / Q000201:enzymology*; D046911:Macromolecular Substances; D010804:Physarum / Q000201:enzymology*; D011487:Protein Conformation; D013045:Species Specificity; D014961:X-Ray Diffraction
D000199:Actins; D046911:Macromolecular Substances; D003851:Deoxyribonucleases