title: The determination of binding parameters when the total and free substrate concentrations are not approximately equal.
abstract: By using a standard graphical method values of Km and V may be found that are independent of the conditions and assumptions that the total substrate concentration approximates to its free concentration and that Km is much larger than the enzyme concentration. The procedure is also applicable to the determination of equilibrium binding parameters of a ligand to a macromolecule.
mesh_terms: D001665:Binding Sites; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D046911:Macromolecular Substances; D008722:Methods; D008956:Models, Chemical
chemical_list: D004798:Enzymes; D008024:Ligands; D046911:Macromolecular Substances