The effect of inhibitors of glycosylation on interferon production in human lymphoblastoid cells.
Interferon production was inhibited in the Namalwa line of human lymphoblastoid cells by treatment with 2-deoxy-D-glucose or D-glucosamine. D-Glucosamine also inhibited protein synthesis and the cells were no longer viable, whereas 2-deoxy-D-glucose allowed protein and RNA synthesis to continue at control rates, and the cells remained fully viable. It is concluded that a glycosylation step is essential for production of lymphoblastoid interferon.
D002051:Burkitt Lymphoma; D002460:Cell Line; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D005944:Glucosamine / Q000494:pharmacology*; D006801:Humans; D007372:Interferons / Q000096:biosynthesis*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis
D003837:Deoxy Sugars; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D007372:Interferons; D003847:Deoxyglucose; D005944:Glucosamine