Evidence for a single species of DNA ligase localized in nuclei of rat liver.
DNA ligase activity was distributed mainly in the extract of nuclei rapidly isolated from rat liver and about 20% of the nuclear activity in the cytoplasmic fraction. The DNA ligases of nuclear extract and cytoplasmic fraction showed similar gel-filtration patterns and the same sedimentation coefficient of 5.5 S. The appearance of 4-S enzyme from nuclei or nuclear extract was partly inhibited by phenylmethylsulfonyl fluoride. These results suggest that the major activity of DNA ligase in rat liver is due to a single species of the enzyme localized in nuclei and that 4-S nuclear DNA ligase reported previously is an artifact arising from 5.5-S enzyme during the isolation procedure.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D011088:DNA Ligases / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology*; D011118:Polynucleotide Ligases / Q000302:isolation & purification*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D011088:DNA Ligases; D011118:Polynucleotide Ligases