title: Delayed-type hypersensitivity measured as an increase of ear thickness in guinea pigs.
abstract: Determination of swelling at an intracutaneous test site in the pinna of the ear of guinea pigs immunized with protein antigens in complete Freund's adjuvant was found to be a more sensitive assay of delayed-type hypersensitivity than the measurement of flank skin erythema and induration. In fact, 100 times less specific antigen was needed to detect 24-hour reactivity in the pinna of the ear. Reactivity early after sensitization (cutaneous basophil hypersensitivity), however, was best revealed as an erythematous lesion of the flank skin 24 h after testing.
mesh_terms: D000818:Animals; D004168:Diphtheria Toxoid / Q000276:immunology; D004423:Ear; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000175:diagnosis*; D007114:Immunization; D008297:Male; D012710:Serum Albumin, Bovine / Q000276:immunology; D012882:Skin Tests / Q000379:methods*
chemical_list: D004168:Diphtheria Toxoid; D012710:Serum Albumin, Bovine