Radioisotopic labelling of endoalveolar surface-active phospholipidic fractions from rabbit pulmonary lavage.
A simple method of detecting endoalveolar tensioactive system fractions employing intravenously injected 131I-triolein has been devised. The tracer has been administered to 7 adult rabbit groups then sacrificed at different time intervals after the injection. Endoalveolar tensioactive system lipidic fractions collected by pulmonary lavage have been separated with thin-layer chromatography and their radioactivity evaluated by scanning plates. Endoalveolar tensioactive system fractions containing considerable amounts of C 18 fatty acids have been selectively labelled.
D000818:Animals; D002855:Chromatography, Thin Layer; D005260:Female; D007457:Iodine Radioisotopes; D008168:Lung / Q000032:analysis*; D008297:Male; D010743:Phospholipids / Q000032:analysis; D011817:Rabbits; D013501:Surface-Active Agents / Q000032:analysis*; D014304:Triolein
D007457:Iodine Radioisotopes; D010743:Phospholipids; D013501:Surface-Active Agents; D014304:Triolein