title: Studies on isolated cloned populations from irradiated human embryonic cell cultures.
abstract: The recovery of substrains with stable chromosome aberrations from irradiated fibroblast culture are reported. Four human fetal cell strains were exposed to 600 rad of gamma rays at 200 rad/min. The efficiency of recovering viable cloned subpopulations was approximately 87%, and the frequency of clones with abnormal chromosomes was 40/100 colonies. G-band chromosome analyses for 34 abnormal substrains are described. Karyotypes of some of the clones with complex rearrangements are also presented. Analyses of a total of 47 aberrant events in the 34 abnormal substrains revealed at 7:1 and a 9:1 translocation-inversion and translocation-deletion ratios, respectively. Five of the abnormal substrains were continuously cultured; all except one showed signs of sensecence toward the end of 44 +/- 10 doublings. Unusual prolonged proliferation capacity was observed in substrain FFS-1-9. The significance of this finding is discussed.
mesh_terms: D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000528:radiation effects*; D002999:Clone Cells; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D007621:Karyotyping; D008168:Lung / Q000648:ultrastructure; D012867:Skin / Q000648:ultrastructure