title: Articular cartilage preservation and storage. II. Mechanical indentation testing of viable, stored articular cartilage.
abstract: Mature rabbit articular cartilage in the form of distal femoral condyles, composite osteoarticular structures, were incubated in the presence of alpha-tocopherol (200 micrograms/ml) over a period of time. Indentation testing and 35S uptake indicate preservation of sustained load carrying capacity and viability, respectively, in the presence of alpha-tocopherol for up to 30 days in organ culture. Condylar cartilage stored in the absence of alpha-tocopherol as well as frozen cartilage demonstrated progressive inability to resist sustained loading over time. Nonoptimal synthetic function apparently occurred in these latter two groups when compared to alpha-tocopherol stored material.
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000503:physiopathology / Q000637:transplantation*; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D004121:Dimethyl Sulfoxide; D004305:Dose-Response Relationship, Drug; D005615:Freezing; D006595:Hexosamines / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D011817:Rabbits; D013314:Stress, Mechanical; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014022:Tissue Survival; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D003470:Culture Media; D006595:Hexosamines; D014810:Vitamin E; D006909:Hydroxyproline; D004121:Dimethyl Sulfoxide