[mRNA sequences complementary to the double-stranded regions of pre-mRNA. Hybridization experiments using RNA mercuriation and chromatography on sulfhydryl-Sepharose].
It was demonstrated that 4--6% of the sequences of mouse cell pure polysomal poly (A)mRNA are able to form stable duplexes during hybridization with the excess of double-stranded structures of nuclear pre-mRNA (double-stranded RNA). Hybridization of mRNA with the excess of mercuriated double-stranded RNA and chromatography of the hybrids on sulfhydril-sepharose was investigated. It was found that only 7--11% of the molecules of polysomal poly(A)+mRNA are able to form thermostable hybrids with sequences of double-stranded RNA. It may be supposed that in the population of mRNA there are relatively few molecules (about 5%) which contain sequences originated from long hairpin structures of pre-mRNA. Moreover, probably, there is a number of mRNA molecules (about 5% of the mRNA population) which are complementary in the entire length to "non-hairpin" double-stranded regions of pre-mRNA.
D001483:Base Sequence; D002846:Chromatography, Affinity; D008628:Mercury; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D011061:Poly A; D011132:Polyribosomes / Q000032:analysis; D012333:RNA, Messenger / Q000096:biosynthesis
D012333:RNA, Messenger; D011061:Poly A; D008628:Mercury
Posledovatel'nosti mPNK, komplementarnye dvuspiral'nym uchastkam pro-nRNK. Gibridizatsiia s ispol'zovaniem merkurirovaniia RNK i khromatografiia na SH-sefaroze.