title: Appearance of 15N-labeled intestinal microbial amino acids in the venous blood of the pig colon.
abstract: Two experiments were done to determine whether pigs possess the ability to absorb amino acids synthesized from urea nitrogen by indigenous microbes in the large intestine. Incorporation of [15N]urea into amino acid fractions of bacterial cells from the rectum and of the deproteinized incubated medium were examined in an experiment in vitro. The isotope was incorporated into 17 amino acids and the ammonia fraction of these samples. The absorption of the microbial amino acids from the colon was investigated by determination of the 15N concentration of the free amino acids in the venous blood of the colon after infusion of the 15N-labeled microorganisms into the cecum. The increase of 15N concentration was also observed in the plasma-free amino acids (threonine, isoleucine, phenylalanine, lysine, histidine, arginine, aspartic acid, serine, alanine, cystine) of the blood from the colic branch of the ileocolic vein. The results of these experiments indicated that pigs have the ability to utilize the microbial amino acids synthesized from urea nitrogen in the large intestine.
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis / Q000097:blood*; D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D002432:Cecum / Q000378:metabolism; D003106:Colon / Q000098:blood supply*; D007408:Intestinal Absorption; D007420:Intestine, Large / Q000382:microbiology; D008297:Male; D013552:Swine / Q000097:blood* / Q000382:microbiology; D014508:Urea / Q000378:metabolism; D014680:Veins
chemical_list: D000596:Amino Acids; D014508:Urea