Membrane abnormalities of pyruvate kinase deficient red cells.
Two experimental systems have been applied to the study of PK-deficient red cells in an attempt to reveal possible membrane abnormalities of these erythrocytes: (1) the glycoprotein self-digestion of intact erythrocytes during in vitro incubation at 37 degrees C; (2) the red cell sensitivity to the lytic action of mouse macrophages. PK-deficient erythrocytes display a more precocious than normal membrane glycoprotein self-digestion and are much more susceptible than normal to the cytotoxic activity of mouse macrophages. This latter effect is more pronounced in young than in old PK-deficient red cells. These observations indicate the existence of membrane abnormality in PK-deficient red cells other than those so far suspected.
D000255:Adenosine Triphosphate / Q000097:blood; D000328:Adult; D000818:Animals; D003602:Cytotoxicity, Immunologic; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology*; D004913:Erythrocytes, Abnormal; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D011770:Pyruvate Kinase / Q000172:deficiency*; D013696:Temperature
D006023:Glycoproteins; D000255:Adenosine Triphosphate; D011770:Pyruvate Kinase