title: Biomembrane cooperative enzymes. In vivo modulation of rat erythrocyte acetylcholinesterase by insulin in normal and diabetic conditions.
abstract: The present study investigated the effect of insulin in vivo on the changes in the cooperativity of a membrane-bound enzyme. The allosteric inhibition by F- of the erythrocyte membrane acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) was studied during intravenous glucose tolerance tests in control and alloxan-induced diabetic rats. In the former group, the value of n decreased from 1.6 to 1.0 whereas it remained about 1.6 in the latter groups. Intravenous injection of insulin (30 U/kg) decreased the values of n in both groups. It is suggested that the in vivo insulin action on membrane cooperative enzymes could also take place in insulin target cells.
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011972:Receptor, Insulin
chemical_list: D007328:Insulin; D011972:Receptor, Insulin; D000110:Acetylcholinesterase