Changes in glycoproteins of liver plasma membranes from rats treated with D-galactosamine.
D-Galactosamine administration to rats (400 mg/kg) by intraperitoneal injection induced biochemical alterations in liver plasma membranes. Alterations were studied 4, 16 and 24 h after D-galactosamine injection. Plasma membrane 5'-mononucleotidase activity decreased to 40% of control values. Carbohydrate composition was significantly changed. After 24 h D-galactosamine administration, the diminution in plasma membrane sialic acids and hexoses reached 30% of control values. As detected by SDS-acrylamide gel electrophoresis, high molecular weight glycoproteins of D-galactosamine-treated plasma membranes were modified. Moreover, the incorporation of [35S]-sulfate into membrane glycoproteins decreased after D-galactosamine administration (40--60% of control). The present results show that biochemical alterations in rat liver plasma membranes appear soon after D-galactosamine injection. Marked changes are observed in cell surface glycoproteins, especially in sialoglycoproteins and sulfated glycoproteins.
D000818:Animals; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005688:Galactosamine / Q000633:toxicity*; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009708:Nucleotidases / Q000378:metabolism; D051381:Rats; D013431:Sulfates / Q000378:metabolism
D006023:Glycoproteins; D013431:Sulfates; D005688:Galactosamine; D009708:Nucleotidases; D005944:Glucosamine