Partial purification and characterization of mammary stimulating factor, a protein which promotes proliferation of mammary epithelium.
This report describes the partial purification and characterization of mammary stimulating factor (MSF), a mitogenic peptide isolated from serum which initiates growth in mouse mammary epithelium. By using ion-exchange chromatography, gel filtration, and isoelectric focusing, MSF was purified 250-fold from porcine serum. It is a heat-stable protein of molecular weight 10,100--10,400 with an isoelectric point of 5.5--6.0. MSF initiates DNA synthesis in vitro in mammary epithelium to a greater extent than in mouse mammary tumor cells (CZF), 3T3 cells, or chick embryo cells. Comparison of the biological, physical, and immunological properties of MSF with other established growth-promoting peptides suggests that MSF is a unique serum factor.
D000818:Animals; D001681:Biological Assay; D001798:Blood Proteins / Q000302:isolation & purification*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004848:Epithelium / Q000378:metabolism; D005260:Female; D008099:Liver / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000302:isolation & purification* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D011955:Receptors, Drug / Q000378:metabolism
D001798:Blood Proteins; D008934:Mitogens; D011955:Receptors, Drug; D004247:DNA