The covalent structure of calf skin type III collagen. IV. The amino acid sequence of the cyanogen bromide peptide alpha 1(III)CB5 (positions 552--788).
The cyanogen bromide peptide alpha 1(III)CB5 is 237 amino acid residues in length and occupies position 552--788 along the alpha 1(III) chain. For sequence analysis alpha 1(III)CB5 was fragmented with hydroxylamine, protease from Staphylococcus aureus V8, trypsin and the arginine-specific enzyme from mouse submaxillary gland. The peptides obtained were separated using molecular and ion exchange chromatography and sequenced with the automated Edman degradation procedure.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003094:Collagen; D003488:Cyanogen Bromide; D006898:Hydroxylamines; D046911:Macromolecular Substances; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases; D012867:Skin / Q000032:analysis*; D013211:Staphylococcus aureus / Q000201:enzymology; D013363:Submandibular Gland / Q000201:enzymology; D014357:Trypsin
D006898:Hydroxylamines; D046911:Macromolecular Substances; D010446:Peptide Fragments; D003094:Collagen; D010447:Peptide Hydrolases; D014357:Trypsin; D003488:Cyanogen Bromide