title: Physical map of bacteriophage BF23 DNA: restriction enzyme analysis.
abstract: Cleavage maps of bacteriophage BF23 DNA have been constructed for the restriction endonucleases SalI (3 fragments), BamHI (5 fragments), EcoRI, (8 fragments), BalI (13 fragments), and HpaI (49 fragments, 32 of which have been ordered). The maps were determined by (i) analysis of deletion mutants, (ii) digestion with two endonucleases, (iii) digestion of isolated fragments with a second enzyme, (iv) analysis of partial digests, and (v) digestion after treatment with lambda exonuclease.
mesh_terms: D002874:Chromosome Mapping; D003090:Coliphages / Q000032:analysis / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D005814:Genes, Viral; D009154:Mutation
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes