Molecular species of interferon induced in mouse L cells by Newcastle disease virus and polyriboinosinic-polyribocytidylic acid.
Interferons were stimulated in mouse L cells by Newcastle disease virus (NDV) or by polyriboinosinic-polyribocytidylic acid poly(rI).poly(rC). These were fractionated by sequential affinity chromatography on bovine plasma albumin (BPA)-Sepharose and on omega-carboxypentyl (CH)-Sepharose. Based on their interaction with CH-Sepharose, interferon induced by NDV was resolved into three major bands of activity (L/NDV-1,2,3) and poly(rI).poly(rC)-interferon into two (L/rI:rC-1,2). These interferon components were purified to a specific activity of 3 X 10(7) to 4 X 10(7) units/mg protein by antibody affinity chromatography and examined by electrophoresis in SDS-polyacrylamide gels. A total of five molecular species was thus identified for NDV-induced interferon and three for poly(rI).poly(rC) induced interferon, as summarized in Table 1. We conclude from our observations that mouse interferons can be produced by L cells in multiple forms with specific physiochemical properties and in proportions determined by the type of agent employed for induction.
D000818:Animals; D002846:Chromatography, Affinity; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D007372:Interferons / Q000032:analysis / Q000096:biosynthesis* / Q000145:classification; D007739:L Cells / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009522:Newcastle disease virus / Q000254:growth & development*; D011070:Poly I-C / Q000494:pharmacology*
D007372:Interferons; D011070:Poly I-C