Changes in chemical composition of chick embryos treated with a beta-xyloside and a lathyrogen.
Nine-day chick embryos were treated with 4-methylumbelliferyl beta-D-xyloside or beta-aminopropionitrile fumarate, and their gross chemical composition was examined one week later. Total DNA was 10--20% less in embryos treated with either drug than it was in control embryos. Xyloside-treated embryos showed marked increases in percent wet weight and in sodium/DNA and chloride/DNA ratios, and small decreases in protein/DNA, hydroxyproline/DNA and sulfate/DNA. None of these parameters was affected in embryos treated with beta-aminopropionitrile. Approximately 85% of the uronic acid of control embryos was present as chondroitin sulfate, with a degree of sulfation of 80% and charge density of 1.8; all of this chondroitin sulfate was covalently linked to peptide and had a number-average molecular weight of 29,300. In embryos treated with beta-xyloside, 90% of the uronic acid was present as chondroitin sulfate, with a degree of sulfation of 40% and charge density ranging from 1 to 2; 27% of this chondroitin sulfate, with an average molecular weight of 25,400, was peptide linked, while 73% was linked to 4-methylumbelliferone and had an average molecular weight of 22,900. The chemical differences between embryos treated with the xyloside and embryos treated with the lathyrogen reinforce the conclusion on morphological grounds that these are distinct syndromes involving different aspects of the extracellular matrix.
D000014:Abnormalities, Drug-Induced / Q000378:metabolism*; D000629:Aminopropionitrile; D000818:Animals; D001835:Body Weight; D002642:Chick Embryo / Q000032:analysis / Q000187:drug effects*; D002809:Chondroitin Sulfates / Q000032:analysis; D004247:DNA / Q000032:analysis; D005110:Extracellular Space / Q000187:drug effects; D006025:Glycosaminoglycans / Q000032:analysis; D006027:Glycosides; D006923:Hymecromone / Q000031:analogs & derivatives; D007842:Lathyrism / Q000139:chemically induced*; D014468:Umbelliferones / Q000031:analogs & derivatives
D006025:Glycosaminoglycans; D006027:Glycosides; D014468:Umbelliferones; D000629:Aminopropionitrile; D006923:Hymecromone; D002809:Chondroitin Sulfates; D004247:DNA