Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport.
A new method for studying membrane transport is presented. High resolution n.m.r. is used to measure the distribution of small molecules between the intracellular and extracellular compartments. The method uses spin-echo techniques and relies on a difference in the magnetic susceptibility of the media inside and outside of cells. It also provides simultaneous information on the metabolic status of the cell. The method is illustrated by a study of alanine and lactate transport in the human erythrocyte.
D000409:Alanine / Q000097:blood; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007773:Lactates / Q000097:blood; D009682:Magnetic Resonance Spectroscopy
D007773:Lactates; D000409:Alanine