title: Protected nucleotide sequences in nuclear ribonucleoprotein.
abstract: The rapidly labeled nuclear ribonucleic acid in human carcinoma cells which is protected by protein from digestion by staphylococcal nuclease (EC 3.1.4.7) has been investigated. A simple and discrete sequence specificity was not found, but the protected RNA fragments are rich in G + C and were shown by fingerprinting to comprise a nonrandom subset of all heterogeneous nuclear ribonucleic acid (hnRNA) sequences enriched in the sequences AGC, GGC, AGGC, and GAGC. There was no detectable enrichment for dougble-stranded RNA in the protected fraction. These data provide the first evidence that the association of any protein with hnRNA is nonrandom with respect to nucleotide sequence.
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis*; D002843:Chromatin / Q000032:analysis; D003110:Colonic Neoplasms; D006801:Humans; D008836:Micrococcal Nuclease; D009698:Nucleoproteins; D012332:RNA, Heterogeneous Nuclear / Q000302:isolation & purification; D012261:Ribonucleoproteins; D012265:Ribonucleotides / Q000032:analysis
chemical_list: D002843:Chromatin; D009698:Nucleoproteins; D012332:RNA, Heterogeneous Nuclear; D012261:Ribonucleoproteins; D012265:Ribonucleotides; D008836:Micrococcal Nuclease