The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe.
The UGA suppressor tRNA produced by Schizosaccharomyces pombe strain sup3-e was purified to homogeneity. It can be aminoacylated with a serine by a crude aminoacyl-tRNA synthetase preparation from S. pombe cells. By combining post-labeling fingerprinting and gel sequencing methods the nucleotide sequence of this tRNA was determined to be: pG-U-C-A-C-U-A-U-G-U-C-ac4C-G-A-G-D-G-G-D-D-A-A-G-G-A-m2G2-psi-U-A-G-A-N-U-U-C-A-i6A-A-psi-C-U-A-A-U-G-G-G-C-U-U-U-G-C-C-C-G-m5C-G-G-C-A-G-G-T-psi-C-A-m1A-A-U-C-C-U-G-C-U-G-G-U-G-A-C-G-C-C-A OH. The anticodon sequence u ca is complementary to the UGA codon.
D000604:Amino Acyl-tRNA Synthetases; D001203:Ascomycota / Q000737:chemistry*; D001483:Base Sequence; D003062:Codon; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger; D012343:RNA, Transfer; D012260:Ribonucleases; D012568:Schizosaccharomyces / Q000737:chemistry* / Q000201:enzymology; D012694:Serine
D003062:Codon; D012333:RNA, Messenger; D012694:Serine; D012343:RNA, Transfer; D012260:Ribonucleases; D000604:Amino Acyl-tRNA Synthetases