[Trypsin immobilization on organo-silica surface].
The methods are suggested for immobilizing trypsin on the organo-silica surface by means of cyanuric chloride and maleic anhydride. The analysis of kinetics of the enzyme carrier coupling shows that at 22 degrees C the immobilization time on the surface of organo-silicas activated with cyanuric chloride and maleic anhydride is 70 and 35 min, respectively. The immobilized trypsin is shown to be more thermostable as compared to the soluble one. The half-life of the immobilized trypsin at 55 degrees C is about 40 h.
D004800:Enzymes, Immobilized / Q000378:metabolism*; D006207:Half-Life; D007700:Kinetics; D008722:Methods; D011485:Protein Binding; D013696:Temperature; D014357:Trypsin / Q000378:metabolism*
D004800:Enzymes, Immobilized; D014357:Trypsin
Immobilizatsiia tripsina na poverkhnosti organokremnezemov.