Abnormal lipid-linked oligosaccharides in class E Thy-1-negative mutant lymphomas.
The glycosylation defect of Thy-1-mutant lymphomas of the class E complementation group has been identified as a block in the synthesis of the lipid-linked oligosaccharide precursor of the asparagine-linked oligosaccharides of glycoproteins. Two major lipid-linked oligosaccharides were isolated from the mutant cells. Both oligosaccharides were smaller than the lipid-linkid oligosaccharides of wild-type lymphomas and, in contrast to the lipid-linked oligosaccharides isolated from wild-type cells, both were resistant to digestion with endoglycosidase H. The oligosaccharides of newly synthesized polypeptides in class E Thy-1-cells were also resistant to endoglycosidase H digestion, providing strong evidence that they are derived from the abnormal lipid-linked oligosaccharides.
D000818:Animals; D002460:Cell Line; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D007519:Isoantigens / Q000235:genetics; D050356:Lipid Metabolism; D008223:Lymphoma / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009154:Mutation; D009363:Neoplasm Proteins / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*
D006023:Glycoproteins; D007519:Isoantigens; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009844:Oligosaccharides