The effect of label affinity on the sensitivity and specificity of a hapten radioimmunoassay: a comparison of three [125I]diphenylhydantoin radioligands with the 14C-labelled drug.
The effect on the sensitivity and specificity of a radioimmunoassay for diphenylhydantoin (DPH)has been investigated using three 125I-labelled tyrosine ester derivatives of DPH having different bridge lengths between the tyrosine moiety and the DPH moiety and 14C-labelled DPH. The results demonstrate that for a hapten which does not completely fill the antibody-binding sites, greatest sensitivity is achieved when the bridge of the iodine label is most dissimilar to that present in the original immunogen, when the hapten and label affinities are nearly equivalent. Greatest specificity is achieved with the label which most resembles the original immunogen. These results illustrate the difficulty of designing satisfactory labels for assays of both high specificity and sensitivity since minimal changes in label structure may produce greatly amplified changes in the subsequent affinity of the label for the antiserum.
D000345:Affinity Labels / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D003429:Cross Reactions; D006241:Haptens; D007457:Iodine Radioisotopes; D010672:Phenytoin; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000276:immunology; D014631:Valerates / Q000276:immunology
D000345:Affinity Labels; D002250:Carbon Radioisotopes; D006241:Haptens; D007457:Iodine Radioisotopes; D014631:Valerates; D012710:Serum Albumin, Bovine; D010672:Phenytoin