title: Influence of superoxide generating system, vitamin E, and superoxide dismutase on radiation consequences.
abstract: During studies of the mechanism by which hemolysis is induced in irradiated human erythrocytes in vitro, several inducements of membrane lipid peroxidation and protective effects of vitamin E (V.E) and superoxide dismutase (SOD) were investigated. Findings were: (1) Before hemolysis, K+ release from erythrocytes induced by radiation stimulated hemolysis but was inhibited by V.E or SOD. (2) Lipid peroxidation of mitochondria induced by Fe3+, ADP, and superoxide (O2-) generating system, and lipid peroxidation of microsome induced by O2- generating system, were also inhibited by V.E or SOD. (3) X-ray or 60Co gamma-ray radiation stimulated lipid peroxidation of liver homogenate, microsome, and liposome. Some of this peroxidation was inhibited by V.E. or SOD. These results suggest that O2- and/or OH formation by radiation induces membrane lipid peroxidation, which causes deterioration of membrane resulting in change of ion permeability and consequent hemolysis.
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D003037:Cobalt Radioisotopes; D005290:Ferric Compounds / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D005720:Gamma Rays; D006461:Hemolysis / Q000528:radiation effects*; D006801:Humans; D008054:Lipid Peroxides / Q000096:biosynthesis / Q000528:radiation effects*; D008099:Liver / Q000528:radiation effects*; D011837:Radiation-Protective Agents; D051381:Rats; D013482:Superoxide Dismutase / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*; D014965:X-Rays
chemical_list: D003037:Cobalt Radioisotopes; D005290:Ferric Compounds; D005296:Ferrous Compounds; D008054:Lipid Peroxides; D011837:Radiation-Protective Agents; D014810:Vitamin E; D000244:Adenosine Diphosphate; D013482:Superoxide Dismutase