title: Activity of mouse macrophages purified by adherence to, and removal from, a plastic surface.
abstract: Mouse peritoneal exudate macrophages were allowed to adhere to plastic Petri dishes and, after washing, were removed by means of EDTA. Cells with the morphology of macrophages were recovered in a fair degree of purity (90-98%) but in low yield (31-34%). The macrophages recovered were fully active in the following ways: re-adherence to glass; pinocytosis of colloidal gold; phagocytosis of opsonized sheep erythrocytes; binding cytophilic antibody; production of lymphocyte activating factor; cytotoxic and cytostatic effects on tumour cells.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002448:Cell Adhesion; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D008222:Lymphokines / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D010587:Phagocytosis; D010873:Pinocytosis; D010969:Plastics
chemical_list: D000906:Antibodies; D008222:Lymphokines; D010969:Plastics