The rapid preparation of peroxisomes from rat liver by using a vertical rotor.
A method is described for the rapid preparation of peroxisomes from rat liver by using sucrose-density-gradient centrifugation in a vertical rotor. The preparation, shown to be virtually free of mitochondrial and microsomal contamination, can be used to study fatty acid metabolism by isolated peroxisomes.
D000818:Animals; D002499:Centrifugation, Density Gradient / Q000379:methods; D008099:Liver / Q000378:metabolism; D008297:Male; D008830:Microbodies / Q000378:metabolism; D009940:Organoids / Q000378:metabolism; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D051381:Rats
D010171:Palmitoyl Coenzyme A