Induction of sister-chromatid exchanges in human lymphocytes and Chinese hamster cells exposed to aflatoxin B1 and N-methyl-N-nitrosourea.
The effect of a 1-h exposure to aflatoxin B1 (AFB1) in inducing sister-chromatid exchange in Chinese hamster ovary (CHO) cells and human lymphocytes in the presence or absence of mixed function oxidase ("S9 mix") was compared. CHO cells were also exposed to a graded series of doses of N-methyl-N-nitrosourea, a powerful inducer of SCE whose action was independent of the presence or absence of S9 mix. CHO and human cells showed a close correlation in response to SCE induction by AFB1 and in both cell systems the additon of mixed function oxidases in the S9 mix resulted in a marked enhancement of action of AFB1.
D000348:Aflatoxins / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D002877:Chromosomes, Human / Q000187:drug effects*; D006224:Cricetinae; D003434:Crossing Over, Genetic; D005260:Female; D006801:Humans; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens / Q000037:antagonists & inhibitors; D010088:Oxidoreductases / Q000494:pharmacology*
D000348:Aflatoxins; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D010088:Oxidoreductases