title: Stimulation of gastric mucosal protein synthesis by different molecular forms of gastrin.
abstract: Protein synthesis in gastric mucosa was studied by measuring the incorporation of labeled amino acids into protein by isolated gastric mucosal ribosomes in a cell-free system. In 48-hour fasted rats, administration of the synthetic analogues pentagastrin, tetragastrin and gastrin-17 or naturally occurring molecular forms of human gastrin (G-14, G-34) markedly enhanced (23-123%) the capacity of the gastric mucosal ribosomes to synthesize endogenous mRNA-directed protein in a cell-free system. In the presence of exogenous mRNA (poly-U), the gastric mucosal ribosomes from the saline-treated controls showed a higher poly(U)-directed protein synthesis, compared to each fo the gastrin-treated groups. The protein/polyphenylalanine ratio which represents a ratio of polysomes to monosomes was found increased in ribosomes from the gastrin-treated groups.
mesh_terms: D000818:Animals; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D005755:Gastrins / Q000494:pharmacology*; D010418:Pentagastrin / Q000494:pharmacology; D014176:Protein Biosynthesis; D051381:Rats; D012270:Ribosomes / Q000378:metabolism; D013758:Tetragastrin / Q000494:pharmacology
chemical_list: D005755:Gastrins; D013758:Tetragastrin; D010418:Pentagastrin