Correction factor for the estimation of plasma glucose synthesis from the transfer of 14C-atoms from labelled substrate in vivo: A preliminary report.
Based on a previously designed method for the estimation of the contribution of C-atoms by acetylcoenzyme A to the hepatic oxaloacetate pool, the loss of 14C-atoms on their way from a precursor to plasma glucose due to 'metabolic exchange' was estimated in normal and diabetic dogs and in normal rats. Due to this loss of 14C-atoms, the rates of gluconeogenesis when calculated from the transfer of 14C-atoms from precursors (other than glycerol) are underestimated by a factor of 2.2 +/- 0.07 in normal, 1.8 +/- 0.05 in diabetic dogs, and by 1.55 +/- 0.04 in normal rats.
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000096:biosynthesis*; D002250:Carbon Radioisotopes; D003920:Diabetes Mellitus / Q000378:metabolism; D004285:Dogs; D008722:Methods; D010071:Oxaloacetates / Q000378:metabolism; D051381:Rats
D001786:Blood Glucose; D002250:Carbon Radioisotopes; D010071:Oxaloacetates; D000105:Acetyl Coenzyme A