Nature of collagen in dermatofibrosarcoma protuberans.
The nature of collagen from 2 cases of dermatofibrosarcoma protuberans was studied. For this purpose, the tumor tissue was carefully separated from adjacent normal dermis. The collagen types comprised in the tumor were identified by CM-cellulose chromatographic and SDS-gel electrophoretic analysis of the component alpha-chains. Semiquantitative evaluation of the relative type III content was established by separation of the cyanogen bromide peptides on gels of 12% polyacrylamide in SDS. These studies showed that dermatofibrosarcoma protuberans contains alpha 1(I)-, alpha 2-, and alpha 1(III)-chains as well, and corresponding type I- and type III-related CNBr peptides. Comparing the collagen from dermatofibrosarcoma protuberans to that of normal skin, the relatively increased type III content in the case of dermatofibrosarcoma protuberans becomes apparent.
D000328:Adult; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005354:Fibrosarcoma / Q000032:analysis* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism; D012878:Skin Neoplasms / Q000032:analysis* / Q000378:metabolism / Q000473:pathology
D010455:Peptides; D003094:Collagen