Side products of routine plasma fractionation. I. Serum cholinesterase (EC 3.1.1.8).
Cholinesterase (EC 3.1.1.8) for intravenous use has been obtained from fraction IV, a by-product of ehtanol fractionation. A four-step procedure has been developed associating precipitation by Rivanol and PEG 4000 with chromatography on DEAE-cellulose. The yield corresponds to 30--35% of the activity found in fraction  IV. Vials containing 5000 U of CHE stabilized by 2.5% albumin have been prepared. The pyrogen-free product is well tolerated in the human.
D002498:Centrifugation; D005591:Chemical Fractionation; D002802:Cholinesterases / Q000097:blood*; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D004975:Ethacridine / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D010949:Plasma; D011092:Polyethylene Glycols / Q000494:pharmacology
D000431:Ethanol; D011092:Polyethylene Glycols; D002802:Cholinesterases; D004975:Ethacridine