Spin-labelling of sialic acid in soluble and cell-surface glycoproteins.
A new, mild method is described for spin-labelling sialic acid residues in situ. The procedure involves the formation of C-1 sialamides and has been applied to a serum glycoprotein, a mucin, tissue sections from human colon, and erythrocyte membrane components. The selectivity of the method and its possible applicability to other types of labelling are discussed.
D003106:Colon / Q000032:analysis; D003497:Cyclic N-Oxides; D004910:Erythrocyte Membrane / Q000032:analysis; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D008722:Methods; D009077:Mucins; D010880:Piperidines; D012794:Sialic Acids / Q000032:analysis*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D006023:Glycoproteins; D009077:Mucins; D010880:Piperidines; D012794:Sialic Acids; D013113:Spin Labels