Long term perifusion of rabbit ovarian follicles.
Preovulatory follicles were dissected from ovaries of estrous rabbits. Four follicles were placed in each chamber of a perifusion system. Medium 199, containing 10% rabbit serum and antibiotics, eluted through the chambers at a rate of 1 ml/10 min. Every 6 h for 36 h, 1 microgram of LH and 50 ng of FSH were injected into each chamber. P4, T and E2 were measured in the medium. P4 exhibited a peak after each of the injections at 6, 12, 18, 24 and 30 h. E2 and T gave major peaks after the injection at 0 time, with minor peaks after the injection at 6 h; thereafter T and E2 secretion gradually decreased to levels below those of baseline.
D000818:Animals; D003338:Corpus Luteum / Q000378:metabolism; D003470:Culture Media; D046508:Culture Techniques; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006065:Gonadotropins, Pituitary / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000494:pharmacology; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis; D011817:Rabbits; D013739:Testosterone / Q000096:biosynthesis; D013997:Time Factors
D003470:Culture Media; D006065:Gonadotropins, Pituitary; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone