title: The effects of trans trans methyl linoleate on the concentration of prostaglandins and their precursors in rat.
abstract: Four groups of weanling male rats were fed a diet containing hydrogenated coconut oil (Treatment A); 9-trans, 12-trans linoleate (trans linoleate, (Treatment B); an equal mixture of 9-cis, 12-cis linoleate (cis linoleate) and trans linoleate (Treatment C); and cis linoleate (Treatment D); respectively for 12 weeks. The level of dietary fat was 11% of calories. Only trace amount of eicosatrienoic acid (C20:3w6) was detected in tissues, (liver, platelets) of rats in Treatments A and B. The level of C20:3w6 in platelet lipids of Treatments C and D was 0.1 and 0.33% respectively. The level of arachidonic acid in rats on Treatments A, B, C and D was 2.0, 1.4, 14.1 and 17.6% for platelet lipids, respectively. The serum levels of prostaglandin PGE1 for Treatments A, B, C and D were 1.10 +/- 0.24, 0.22 +/- 0.02, 3.51 +/- 0.58 and 5.69 +/- 0.59 ng/ml, respectively and 2.19 +/- 0.85, 0.15 +/- 0.03, 11.64 +/- 2.63 and 24.89 +/- 4.35 ng/ml for PGE2, respectively. Thus feeding trans linoleate to rats apparently caused decreased biosynthesis of PGs resulting from decreased levels of precursor acids. This was apparently due to the inhibition of conversion of cis linoleate to longer chain polyunsaturated fatty acids. The results indicate that the availability of precursor acids is one limiting factor in PG biosynthesis in rats, and small differences in the level of precursor acids, affected by dietary trans fatty acids may cause large variations in amounts of PGs synthesized.
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D004041:Dietary Fats / Q000008:administration & dosage; D005227:Fatty Acids / Q000032:analysis; D008041:Linoleic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000378:metabolism; D008297:Male; D011453:Prostaglandins / Q000096:biosynthesis* / Q000097:blood; D011458:Prostaglandins E / Q000096:biosynthesis / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D008041:Linoleic Acids; D008055:Lipids; D011453:Prostaglandins; D011458:Prostaglandins E