A cholecystokinin-like peptide in crude substance P from human and bovine brain.
The subfractions of crude substance P (SP), Fa, Fb, and Fc, were assayed for SP-like activity (SP-LA) and cholecystokinin octapeptide-like activity (CCK-8-LA) using radioimmunoassays (RIAs) and bioassays. Crude SP preparations from total human and bovine brains, cortex, and subcortex (amygdala, substantia nigra) contain CCK-8-LA as well as SP-LA. Most of SP-LA was contained in Fb, whereas most of CCK-8-LA was in Fc. Fa gave ambiguous results. Pharmacological results previously obtained with Fc may reflect the activity of CCK-like peptide(s).
D000818:Animals; D001681:Biological Assay; D001923:Brain Chemistry; D002417:Cattle; D002766:Cholecystokinin / Q000032:analysis* / Q000494:pharmacology; D005704:Gallbladder / Q000187:drug effects; D006168:Guinea Pigs; D006801:Humans; D007082:Ileum / Q000187:drug effects; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D010455:Peptides / Q000032:analysis*; D011863:Radioimmunoassay; D013373:Substance P / Q000032:analysis*; D014649:Vas Deferens / Q000187:drug effects
D007141:Immunoglobulin Fc Fragments; D010455:Peptides; D013373:Substance P; D002766:Cholecystokinin