[Detection of rubella specific IgM on gel filtration through Sephadex G 200: use of dithiothreitol and limits with MnCl2-heparine pretreatment (author's transl)].
The detection of rubella haemagglutination inhibiting antibody, in the IgM fraction of the serum, on gel filtration through Sephadex G 200, needs precautions to exclude false results. Treatment with dithiothreitol is a satisfactory method for confirming the content of rubella IgM antibody. The failure of MnCl2-heparin pretreatment to remove non specific inhibitors of rubella hemagglutinin is unfrequent (7/108) and so do be repeated. Rarely (1/108) aggregated IgG fractionates with IgM and yield false positive results.
D000914:Antibodies, Viral / Q000032:analysis* / Q000494:pharmacology; D002850:Chromatography, Gel; D003864:Depression, Chemical; D004229:Dithiothreitol / Q000276:immunology; D006384:Hemagglutination / Q000187:drug effects; D006493:Heparin / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D008345:Manganese / Q000276:immunology; D012412:Rubella virus / Q000276:immunology*
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D008345:Manganese; D006493:Heparin; D004229:Dithiothreitol
Détermination des anticorps rubéoliques de classe IgM par gel-filtration sur Sephadex G 200. Utilisation du dithiothréitol et limites du traitement à l'héparine-chlorure de manganèse.