Factors that influence the process of 51chromium labeling of human granulocytes isolated from blood by counterflow centrifugation.
The uptake of 51chromium by human granulocytes isolated from blood by counterflow centrifugation is influenced by the composition of the suspension medium, the incubation period, the temperature and the amount of 51chromium used. Counterflow centrifugation-elutriation isolated approximately 98 per cent of the granulocytes from the blood. Acid-citrate-dextrose (ACD) plasma, citrate-phosphate-dextrose (CPD) plasma and heparinized plasma as resuspension media produced similar labeling results, whereas phosphate-buffered saline resulted in three times greater uptake in 51chromium. The greatest uptake of 51chromium (4%) was achieved after incubation of 37 C for one hour in autologous plasma, and granulocyte integrity and metabolic function in vitro were not adversely affected.
D001786:Blood Glucose; D001793:Blood Preservation; D002469:Cell Separation; D002498:Centrifugation; D002860:Chromium Radioisotopes; D002951:Citrates / Q000097:blood; D006098:Granulocytes / Q000378:metabolism*; D006493:Heparin / Q000097:blood; D006801:Humans; D010101:Oxygen Consumption; D010710:Phosphates / Q000097:blood; D013696:Temperature; D013997:Time Factors
D001786:Blood Glucose; D002860:Chromium Radioisotopes; D002951:Citrates; D010710:Phosphates; D006493:Heparin