In vitro hexosamine depletion of intact articular cartilage by E-prostaglandins: prevention by chloroquine.
Short-term incubations of intact canine articular cartilage slices with prostaglandins E1 and E2 caused significant losses of hexosamine from cartilage matrix compared to controls. Chloroquine, an inhibitor of DNA primer, prevented this prostaglandin-induced hexosamine depletion. These data suggest that E-prostaglandins may degrade articular cartilage directly through DNA-dependent RNA synthesis of cathepsin-like proteases. Catabolism of articular cartilage probably involves degradation of existing matrix in addition to inhibition of synthesis.
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism*; D002738:Chloroquine / Q000494:pharmacology*; D004285:Dogs; D006595:Hexosamines / Q000378:metabolism*; D066298:In Vitro Techniques; D007719:Knee Joint; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D013997:Time Factors
D006595:Hexosamines; D011459:Prostaglandins E, Synthetic; D012313:RNA; D002738:Chloroquine; D010447:Peptide Hydrolases