title: The action mechanism of the purified platelet aggregation principle of Trimeresurus mucrosquamatus venom.
abstract: The minimal concentration of the platelet aggregation principle (Platelet Aggregoserpentin, PAS) necessary to induce platelet aggregation was 10 ng/ml, about one-hundredth of that of the crude venom. PAS induced the release of platelet factors 3 and 4 from platelets, but the released platelet factor 3 was easily inactivated by the anti-phospholipid effect of PAS. Pretreatment of platelets with neuraminidase potentiated PAS-induced platelet aggregation. PAS-induced platelet aggregation was independent on released ADP; it could occur in the ADP-removing systems, such as apyrase or a combination of phosphoenolpyruvate and pyruvate kinase. However, PAS-induced platelet aggregation could be inhibited by adenine nucleotides and adenosine. PAS-induced platelet aggregation was inhibited by some anti-inflammatory agents, antimalarial drugs, local anesthetics, antihistamine and smooth muscle relaxants. After deaggregation of PAS-treated platelets, thrombin and sodium arachidonate could further induce platelet aggregation, but ADP and second dose of PAS could not. It is concluded that PAS-induced platelet aggregation is due to prostaglandin synthesis. Recent literatures on the mechanism of platelet aggregation were surveyed and the actions of PAS were discussed.
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D001081:Apyrase / Q000378:metabolism; D001095:Arachidonic Acids / Q000494:pharmacology; D009439:Neuraminidase / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010977:Platelet Factor 3; D010978:Platelet Factor 4; D011817:Rabbits; D012910:Snake Venoms / Q000494:pharmacology*
chemical_list: D000962:Antimalarials; D001095:Arachidonic Acids; D012910:Snake Venoms; D010977:Platelet Factor 3; D010978:Platelet Factor 4; D010728:Phosphoenolpyruvate; D009439:Neuraminidase; D001081:Apyrase