Effect of sodium fluoride on protein and DNA synthesis, ornithine decarboxylase activity, and polyamine content in LS cells.
Sodium fluoride exhibited a dose dependent inhibitory effect on protein and DNA synthesis at concentrations from 1.3 mM in growing LS cells. The activity of ornithine decarboxylase (ODC) was slightly stimulated by 0.5 mM-NAF, but inhibited at 1.3 mM and above. The reduced enzyme activity seemed to be due to a reduced de novo formation of the enzyme caused by an inhibition of the protein synthesis. In spite of a reduction of ODC-activity, fluoride had no effect on the cellular polyamine content during the experimental period (10 hours).
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism; D005459:Fluorides / Q000494:pharmacology*; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D014176:Protein Biosynthesis; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000494:pharmacology*
D003470:Culture Media; D011073:Polyamines; D012969:Sodium Fluoride; D004247:DNA; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D005459:Fluorides