The association between major sialoglycoprotein and spectrin of human erythrocyte membranes as detected by fluorescence resonance energy transfer.
The interactions among the major sialoglycoprotein and peripheral proteins of human erythrocyte membranes were investigated by the long range resonance energy transfer between different fluorescent moieties separately conjugated to proteins. Consequently, direct association between the major sialoglycoprotein and spectrin was observed and divalent cations were required for their association.
D004735:Energy Transfer; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D008565:Membrane Proteins / Q000032:analysis*; D012795:Sialoglycoproteins / Q000097:blood*; D013049:Spectrin / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D008565:Membrane Proteins; D012795:Sialoglycoproteins; D013049:Spectrin