Self-association of lyophilized horse liver alcohol dehydrogenase.
The molecular weights of lyophilized and non-lyophilized horse liver alcohol dehydrogenase have been compared by quasi-elastic light scattering, and ultracentrifugation. Whereas the non-lyophilized enzyme has the expected molecular weight of 78 000, the lyophilized enz)me has an initial molecular weight of about 10(6) which increases with time by an endothermic process. This result shows that any physical measurement using lyophilized liver alcohol dehydrogenase to investigate the enzyme mechanism, which relies upon the molecular size, will be invalid.
D000429:Alcohol Oxidoreductases; D000818:Animals; D005612:Freeze Drying; D006736:Horses; D008099:Liver / Q000201:enzymology; D046911:Macromolecular Substances; D008433:Mathematics; D008970:Molecular Weight
D046911:Macromolecular Substances; D000429:Alcohol Oxidoreductases