[Immunological and sedimentation analysis of UV-irradiated T4 phage DNA containing 5-bromouracil].
Conversions resulting from UV-irradiation of 5-bromouracil DNA of T4 phage were studied. The use of immunological and sedimentation techniques revealed that UV dose ranging from 1.4.10(2) to 1.4.10(3) erg/mm2 induced unwinding and single-stranded breaks in 5-bromouracil DNA which are not accompanied by debromination. Beginning with 4.10(3) erg/mm2 debromination but not degradation of DNA is detected. Doses higher than 4.10(3) erg/mm2 lead to extensive degradation of 5-bromouracil T4 DNA.
D001976:Bromouracil; D003090:Coliphages / Q000032:analysis*; D004279:DNA, Viral / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D007118:Immunoassay; D014461:Ultracentrifugation; D014466:Ultraviolet Rays
D004279:DNA, Viral; D001976:Bromouracil
Immunologicheskiĭ i sedimentatsionnyĭ analiz UF-obluchennoĭ DNK faga T4, soderzhashcheĭ 5-bromupatsil.