[Isolation and purification of bovine and porcine cerebral cathepsin D].
Cathepsin D was isolated from the grey matter of bovine and porcine large cerebral hemispheres and purified by affinity chromatography on haemoglobin--Sepharose. The isolation and purification of the enzyme also included: acidic extraction, precipitation by ammonium sulfate, dialysis, affinity chromatography, concentration and gel-chromatography on Sephadex G-100. The degree of purification of bovine cerebral enzyme was 3280. The Km value for the enzyme was 2,06 . 10(-5) M. The purified enzyme from bovine brain showed three major and two minor adjacent bands, possessing the cathepsin D activities. The purified enzyme from porcine brain showed only one protein band.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002403:Cathepsins / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity; D007700:Kinetics; D013045:Species Specificity; D013552:Swine
D002403:Cathepsins
Vydelenie i ochistka katepsina D iz kory golovnogo mozga byka i svin'i.