Kinetic vitreous fluorophotometry in experimental diabetes.
Kinetic vitreous fluorophotometry was used to measure dynamic alterations in blood-retinal barrier function. Normal hooded rats were compared with diabetic animals before and after insulin treatment. Rats with streptozocin-induced diabetes demonstrated significantly longer (P less than .001) half-periods of fluorescein loss from the vitreous when compared with controls, and insulin treatment significantly reduced (P less than .001) the mean half-period toward control values without normalization of serum glucose. These results suggest that kinetic vitreous fluorophotometry is a good indicator of blood-retinal barrier function and that sufficient amounts of insulin may be more important than normal blood glucose levels in recovery of altered barrier function in diabetes.
D000818:Animals; D001693:Biological Transport, Active; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D003930:Diabetic Retinopathy / Q000209:etiology* / Q000503:physiopathology*; D005452:Fluoresceins / Q000008:administration & dosage / Q000378:metabolism; D007275:Injections, Intravenous; D008297:Male; D051381:Rats; D012171:Retinal Vessels / Q000503:physiopathology*; D013050:Spectrometry, Fluorescence; D014822:Vitreous Body / Q000378:metabolism*
D005452:Fluoresceins