A nuclear precursor to human gamma-globin messenger RNA.
RNA isolated from human foetal liver at an erythroid stage of development was transferred from methyl mercury hydroxide agarose gels to diazobenzyloxymethyl paper. A 16S RNA species containing gamma-globin mRNA sequences was identified by hybridisation to 32P-labelled human gamma-globin cDNA recombinant plasmid pHgamma GI. This RNA is found only in the cell nucleus and is polyadenylated. Human globin gene transcription is thus analagous to that in mouse: interventing sequences are transcribed and processed from the pre-mRNA in the nucleus after polyadenylation.
D000328:Adult; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism; D005260:Female; D005333:Fetus; D005914:Globins / Q000096:biosynthesis*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D012156:Reticulocytes / Q000378:metabolism; D014981:Xenopus
D004274:DNA, Recombinant; D012333:RNA, Messenger; D005914:Globins