DNA-dependent RNA polymerase III from Acanthamoeba castellanii: comparison of the catalytic properties of the trophozoite and cyst enzymes.
DNA-dependent RNA polymerase III was partially purified from trophozoites and immature cysts of the small soil ameba, Acanthamoeba castellanii. In contrast to the active modulation of the variety of transfer RNA species which are transcribed during encystment, no difference was found in the chromatographic or catalytic properties of the enzyme responsible for their transcription.
D000546:Amanitins / Q000494:pharmacology; D000656:Amoeba / Q000201:enzymology* / Q000235:genetics; D000818:Animals; D002384:Catalysis; D002413:Cations, Divalent; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D009994:Osmolar Concentration; D012320:RNA Polymerase III / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D012343:RNA, Transfer / Q000096:biosynthesis; D013698:Templates, Genetic; D014158:Transcription, Genetic
D000546:Amanitins; D002413:Cations, Divalent; D012343:RNA, Transfer; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III