[A cell-culture assay for the detection of mycotoxins. I. Investigations with pure toxins (author's transl)].
For the detection of mycotoxins by a biological assay cells were seeded in Microtiter-plates together with toxin. After 24 h and 6 days of culture cells were examined visually for cytocidal effects, growth inhibition, and morphological aberrations. Three human and 2 porcine cell-lines were compared as to their reaction with 16 mycotoxins and 9 organic chemicals. The results indicate that the toxin-activating systems differ with type and origin of cells. This suggests the use of several selected cell-lines in parallel for testing of microbially derived food additives for possible contamination by mycotoxins.
D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D009183:Mycotoxins / Q000032:analysis*; D013552:Swine
D009183:Mycotoxins
Ein Zellkulturtest für den Nachweis von Mykotoxinen I. Untersuchungen an Reinsubstanzen.