title: Condensation of all human chromosomes in phase G2 and early mitosis can be drastically inhibited by 33258-Hoechst treatment.
abstract: Condensation of human chromosomes in phase G2 and early mitosis is inhibited by the fluorochrome 33258-Hoechst. This inhibitory effect is most apparent in primary diploid fibroblasts and lymphoblasts and least pronounced in peripheral blood lymphocytes. Condensation of the human Y chromosome, which contains a large heterochromatic region rich in A-T base pairs, is drastically inhibited by 33258-Hoechst treatment of fibroblasts and lymphoblasts. The difference in sensitivity of human chromosomes in different cell types to 33258-Hoechst probably reflects differences in the cell-membrane permeabilities to 33258-Hoechst.
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002877:Chromosomes, Human / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology; D006367:HeLa Cells / Q000166:cytology; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D008677:Metaphase / Q000187:drug effects*; D051379:Mice; D008938:Mitosis / Q000187:drug effects*; D014998:Y Chromosome / Q000187:drug effects
chemical_list: D001562:Benzimidazoles