Isolation and characterization of a heptaglycosylceramide from bovine erythrocyte membranes.
A heptaglycosylceramide was isolated from bovine erythrocyte membranes. The structure was characterized to be Gal(alpha 1-3)Gal(beta 1-4)GlcNAc(beta1-3)Gal(beta 1-4)Glc-NAc(beta 1-4)al(beta 1-4)GlcCer. A hexaglycosylceramide that has the same sequence except for the terminal alpha-galactosyl unit has also been isolated. We have previously found that gangliosides isolated from bovine erythrocyte membranes contain a keratan sulfate type repeating unit --[3Gal(beta 1-4)-GlcNAc beta]--n. This study shows that the keratan sulfate type repeating unit is also present in the neutral glycosphingolipids of bovine erythrocyte membranes.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D006026:Glycoside Hydrolases; D006028:Glycosphingolipids / Q000097:blood* / Q000302:isolation & purification; D013110:Sphingosine / Q000032:analysis
D005227:Fatty Acids; D006028:Glycosphingolipids; D006026:Glycoside Hydrolases; D013110:Sphingosine