Isolation and structure determination of enzymatically formed styrene oxide glutathione conjugates.
When styrene oxide was incubated with glutathione in the presence of rat liver cytosolic fraction, two conjugates were formed. Structural investigation by mass spectrometry (MS), proton magnetic resonance (PMR) analysis and chemical fragmentation showed the presence of two positional isomers, namely S-(1-phenyl-2-hydroxyethyl)glutathione and S-(2-phenyl-2-hydroxyethyl)glutathione in a ratio of approx. 60 : 40.
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D004852:Epoxy Compounds / Q000378:metabolism*; D004988:Ethers, Cyclic / Q000378:metabolism*; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats
D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D005978:Glutathione