Use of dextran 150 as a macromolecular agent to improve granulocyte yields on the intermittent flow blood cell separator (Haemonetics Model 30).
Leukaphereses were performed on the intermittent flow cell separator (Haemonentics Model 30) using citrated dextran 150 as a macromolecular agent to improve separation of granulocytes. A mean of 1.77 X 10(10) granulocytes and 7.73 X 10(11) platelets were obtained without steroid stimulation and 2.65 X 10(10) granulocytes and 8.91 X 10(11) platelets were obtained with steroid stimulation of donors. There were no significant adverse reactions to dextran.
D002469:Cell Separation / Q000379:methods*; D003911:Dextrans / Q000494:pharmacology*; D006098:Granulocytes; D006801:Humans; D007937:Leukapheresis
D003911:Dextrans