[Biosynthesis of hyaluronic acid in parietal pleura of the rabbit (author's transl)].
The parietal pleura of rabbits was incubated with 14C-glucosamine. It was found that 14C-glucosamine was incorporated into the fraction of crude glycosaminoglycans. Then the crude glycosaminoglycans were fractionated by using specific mucopolysaccharide-lyases (hyaluronidase from streptomyces hyalurolytics, chondroitinase AC and chondroitinase ABC). As a result, evidence was obtained that hyaluronic acid was synthesized in parietal pleura and was released into the surroundings.
D000818:Animals; D002250:Carbon Radioisotopes; D005260:Female; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D010994:Pleura / Q000378:metabolism*; D011817:Rabbits
D002250:Carbon Radioisotopes; D006025:Glycosaminoglycans; D006820:Hyaluronic Acid; D005944:Glucosamine
Die Biosynthese von Hyaluronsäure im Pleuragewebe des Kaninchens.