Crystallization studies in a urothelial-lined living test tube (the catheterized female rat bladder). I. Calcium oxalate crystal adhesion to the chemically injured rat bladder.
We developed an in vivo technique to study crystallization in a urothelial-lined "living test tube" by employing the catheterized female rat bladder. Calcium oxalate crystals adherent to the bladder urothelium were distinguished from free or intraluminal crystals. Chemical injuries of the urothelium induced by either 0.1 N HCL or 5 per cent Triton X 100 in saline resulted in marked calcium oxalate crystal adhesion to the injured urothelium. Control bladders without urothelial injury remained free of adherent crystals but formed large numbers of small free crystals relatively uniform in size. Histologic and fresh microscopic evaluations of urothelial injury and crystal adhesion are presented.
D000818:Animals; D002129:Calcium Oxalate / Q000378:metabolism* / Q000652:urine; D002404:Catheterization; D003460:Crystallization; D005260:Female; D006851:Hydrochloric Acid; D008722:Methods; D010784:Photomicrography; D011092:Polyethylene Glycols; D051381:Rats; D001745:Urinary Bladder Diseases / Q000139:chemically induced / Q000150:complications / Q000378:metabolism*; D014545:Urinary Calculi / Q000150:complications / Q000378:metabolism
D002129:Calcium Oxalate; D011092:Polyethylene Glycols; D006851:Hydrochloric Acid