The evaluation of a radioimmunoassay for phenothiazines and thioxanthenes using an iodinated tracer.
Antisera have been raised in rabbits to the immogen 2-trifluoromethylphenothiazine-10-beta-propionate bovine serum albumin. An [125I]-labelled tyrosine methyl ester derivative of the immunogen precursor has been synthesised and used with the antisera to develop a simple, precise and sensitive radioimmunoassay for phenothiazines and thioxanthenes bearing 2-trifluoromethyl substituents. The assay can detect 0.4 ng/ml of fluphenazine, trifluorperazine or (Z)-flupenthixol in 100 microliter of human serum without interference from their sulphoxide or 7-hydroxylated metabolites. An acceptable correlation between this assay and an established fluphenazine radioimmunoassay using commercial [3H]fluphenazine has been obtained for plasma samples.
D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D005475:Flupenthixol / Q000276:immunology; D005476:Fluphenazine / Q000276:immunology; D006801:Humans; D007457:Iodine Radioisotopes; D010640:Phenothiazines / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay; D013892:Thioxanthenes / Q000276:immunology*; D014268:Trifluoperazine / Q000276:immunology
D007457:Iodine Radioisotopes; D010640:Phenothiazines; D013892:Thioxanthenes; D014268:Trifluoperazine; D005475:Flupenthixol; D005476:Fluphenazine