Identification of autoanti-Thi-1 antibody in the sera of BALB/c mice immune to P1798 lymphoma.
The specificity was established for the anti-thymocyte autoantibody which appeared in BALB/c mice (BAA) during immunization to the P 1798 lymphoma. The results indicate that BAA is specific for the Thy-1 alloantigen of murine T cells. By immunoprecipitation of 125I-labeled A/J thymocyte surface antigens from freeze-thaw lysates and electrophoretic resolution of the immunoprecipitates on sodium dodecyl sulfate polyacrylamide gels, the BAA and congenic anti-Thy-1.1 or 1.2 were shown to be specific for determinants on the same antigens. Specificity of BAA for Thy-1.2 was shown also quantitative absorption studies. Coating P 1798 lymphoma cells with either BAA or anti-Thy-1.2 reduced the absorptive capacity of these cells for the same or the reciprocal antiserum. Coating P 1798 with an antiserum to P 1798 which was indifferent to the Thy-1.2 alloantigen did not diminish the absorptive capacity of these cells for either BAA or anti-Thy-1.2. These studies have provided firm evidence that BAA is specific for determinants on the Thy-1 alloantigen.
D000042:Absorption; D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007106:Immune Sera; D007519:Isoantigens / Q000276:immunology; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D013950:Thymus Gland / Q000276:immunology*
D001323:Autoantibodies; D007106:Immune Sera; D007519:Isoantigens