Microchromatographic quantitation of fetal hemoglobin in patients with sickle cell disease.
A microchromatographic procedure (Isolab Fast Hb Test System) which was developed for the quantitation of Hb AI (10) has been found useful for the quantitation of Hb F in samples that contain Hb S and/or Hb C but no Hb A (% Fmicro). This method has been evaluated through analyses of known mixtures of Hb F and Hb S. The Hb Fmicro levels in patients with sickle cell anemia and related conditions were compared with results obtained by alkali denaturation (% FAD) and conventional DEAE-cellulose chromatography (% FDE). This microchromatographic technique is a fast, simple, and sensitive method for Hb F quantitation in patients with Sickle cell disease.
D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood*; D002845:Chromatography / Q000379:methods; D002848:Chromatography, DEAE-Cellulose; D005319:Fetal Hemoglobin; D006451:Hemoglobin, Sickle; D006461:Hemolysis; D006801:Humans; D007231:Infant, Newborn; D013997:Time Factors
D006451:Hemoglobin, Sickle; D005319:Fetal Hemoglobin