Correlation between glucocorticoid receptor and cytolytic response of murine lymphoid cell lines.
By fusion between murine thymoma lines which are either homozygous (r+/r+ or r-/r-) or hemizygous (r+/r-) for the glucocorticoid receptor structural gene r, hybrid clones have been obtained which carry one, two three, or four copies of the r+ allele. These hybrids contain different amounts of normal glucocorticoid receptor as the result of a r+ gene dosage effect. Measurements of the cytolytic response of these hybrids to dexamethasone indicate a tight correlation between receptor content and sensitivity.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology*; D004351:Drug Resistance; D005838:Genotype; D006822:Hybrid Cells / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D011987:Receptors, Steroid / Q000235:genetics; D013945:Thymoma / Q000188:drug therapy* / Q000378:metabolism; D013953:Thymus Neoplasms / Q000188:drug therapy* / Q000378:metabolism
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone