RNA synthesis in newly isolated and cultivated mesophyll protoplasts.
Tobacco leaf mesophyll protoplasts exhibit low incorporation of [3H]uridine and 32P into RNA, up to 12 h of cultivation, irrespective of the presence of phytohormones. After 24 h of cultivation a dramatic increase in RNA synthesis is observed; it is the highest in the heterogeneous nucleoplasmic RNA fraction. The protoplasts cultivated in the absence of phytohormones show lower incorporation of precursors.
D046508:Culture Techniques; D007553:Isotope Labeling; D010937:Plant Growth Regulators / Q000494:pharmacology; D010947:Plants, Toxic; D011523:Protoplasts / Q000378:metabolism*; D012332:RNA, Heterogeneous Nuclear / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics*; D014158:Transcription, Genetic; D014316:Tritium; D014529:Uridine
D010937:Plant Growth Regulators; D012332:RNA, Heterogeneous Nuclear; D012335:RNA, Ribosomal; D014316:Tritium; D014529:Uridine