[Synchronous conformational oscillations in the titer of sulfhydryl groups in protein solutions. Reversible oxidation as a possible cause of this phenomenon].
In solutions of creatine kinase, actin and lactic dehydrogenase oscillations of SH-groups are revealed by three independent methods using three different reagents AgNO3, PCMB, DTNB. The variations of the SH-group titre remain after denaturation of the individual protein solution portions. In the solutions of predenaturated proteins no titre oscillations are observed. Possible cause of the observed oscillations of SH-groups titre may be reversible SH--SS transformation.
D000199:Actins; D002729:Chloromercuribenzoates; D003402:Creatine Kinase; D006146:Guanidines; D006593:Hexokinase; D007770:L-Lactate Dehydrogenase; D010084:Oxidation-Reduction; D011487:Protein Conformation; D011489:Protein Denaturation; D011770:Pyruvate Kinase; D012996:Solutions
D000199:Actins; D002729:Chloromercuribenzoates; D006146:Guanidines; D012996:Solutions; D007770:L-Lactate Dehydrogenase; D006593:Hexokinase; D011770:Pyruvate Kinase; D003402:Creatine Kinase
Sinkhronnye konformatsionnye kolebaniia titra sul'fgidril'nykh grupp v rastvorakh belkov. Obratimoe okislenie kak vozmozhnaia prichina étogo iavleniia.