Vascular casting. A new method for the study of the arterial endothelium.
Vascular casts of the rat aorta were made using modified Batson's No. 17 anatomical corrosion compound. The outline of the silver-stained endothelial cell boundaries were clearly visible on the cast when examined using both scanning electron and light microscopy. The physical properties of the injectate were determined. The exothermic nature of the polymerization of the injectate did not grossly damage the endothelial cells. A method was developed for recovery, without damage, of the arterial tissue surrounding the cast. The technique of vascular casting appears to be a powerful tool for the study of the arterial endothelium which avoids shrinkage artifacts and maintains 3-dimensional geometry.
D000818:Animals; D001012:Aorta, Abdominal / Q000502:physiology* / Q000648:ultrastructure; D002370:Casts, Surgical; D004727:Endothelium / Q000502:physiology / Q000648:ultrastructure; D008722:Methods; D008855:Microscopy, Electron, Scanning; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D051381:Rats