In vivo and in vitro studies on endothelial regeneration.
Endothelial regeneration was studied in rabbit aorta after intra-arterial balloon catheterization. Most of the regenerated endothelium originated from existing branches which was assessed by the Evans-blue uptake pattern and confirmed by transmission and scanning electron microscopy. Glucocorticoid treatment enhanced re-endothelialization whereas hyperlipemic diet inhibited. Sera from minipigs fed an atherogenic diet consistently have less ability than sera from control pigs to stimulate in vitro the regeneration of wounded endothelium-like monolayers of 3T3-B cells. The deficiency is probably due to an inhibitor which appears and disappears with changes in the diet.
D000818:Animals; D001011:Aorta / Q000293:injuries / Q000502:physiology* / Q000648:ultrastructure; D002460:Cell Line; D002465:Cell Movement; D004727:Endothelium / Q000502:physiology / Q000648:ultrastructure; D006949:Hyperlipidemias / Q000097:blood; D007668:Kidney; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D012038:Regeneration; D013552:Swine / Q000097:blood