title: Erythrocyte membrane autophosphorylation in Duchenne muscular dystrophy: effect of two methods of erythrocyte ghost preparation on results.
abstract: We have compared the effects of two slightly different methods of erythrocyte ghost preparation on membrane autophosphorylation in Duchenne muscular dystrophy. Addition of 1 mmol/l EDTA to two of the washing steps altered the differences between patients and controls. These data support the known sensitive interrelationship of Ca2+ and erythrocyte membrane autophosphorylation, underline the importance of strict adherence to methodology when comparing data, and have suggested a new direction of investigation.
mesh_terms: D002118:Calcium / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D006801:Humans; D008297:Male; D008722:Methods; D009136:Muscular Dystrophies / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D002118:Calcium