Sterol synthesis. High-pressure liquid chromatography of C27 sterol precursors of cholesterol.
Conditions have been described which permit the rapid (approximately 3.5 hr) column chromatographic separation of the acetate derivatives of a number of C27 sterol precursors of cholesterol differing only in the number and position of double bonds in the sterol nucleus. On columns containing muPorasil with hexane-benzene 9:1 as the eluting solvent, acetate derivatives of the delta5, delta8(14), delta8, delta7, delta8,7, delta 8,14, and delta7,14 sterols were separated.
D000085:Acetates; D002784:Cholesterol; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013261:Sterols / Q000302:isolation & purification*
D000085:Acetates; D013261:Sterols; D002784:Cholesterol