High-pressure liquid chromatographic analysis of ticrynafen and one of its metabolites in urine and serum.
A method is described for the extraction of ticrynafen, a new hypotensive agent, and its reduced metabolite from serum and urine. Drug-related material is extracted from biological fluids with ether under strongly acidic conditions and then back-extracted into an alkaline aqueous phase, which is subjected to high-pressure liquid chromatographic analysis. Separations are performed on a reversed-phase column with a mobile phase consisting of phosphate buffer-acetonitrile. This accurate and reproducible method measures serum concentrations of ticrynafen and its reduced metabolite as low as 1.0 and 0.4 microgram/ml, respectively.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006016:Glycolates / Q000032:analysis*; D006801:Humans; D008722:Methods; D013989:Ticrynafen / Q000032:analysis* / Q000097:blood / Q000652:urine; D013997:Time Factors
D006016:Glycolates; D013989:Ticrynafen