A screening test for the malignant hyperpyrexia phenotype using suxamethonium-induced contracture of muscle treated with caffeine and its inhibition by dantrolene.
Suxamethonium induced a contracture in caffeine pretreated human muscle in vitro. The contracture was significantly greater (P less than 0.001) with MHS muscle compared with muscle from normal subjects. This reaction is now used as an additional screening test for the MHS phenotype. The contracture was prevented by pretreatment with dantrolene.
D000293:Adolescent; D000328:Adult; D002110:Caffeine / Q000494:pharmacology*; D002648:Child; D003620:Dantrolene / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D008305:Malignant Hyperthermia / Q000175:diagnosis* / Q000235:genetics; D008875:Middle Aged; D009119:Muscle Contraction / Q000187:drug effects*; D010641:Phenotype; D013390:Succinylcholine / Q000037:antagonists & inhibitors
D002110:Caffeine; D003620:Dantrolene; D013390:Succinylcholine