A simple technique for nonsurgical embryo transfer in mice.
A simple method was developed for nonsurgical transfer of mouse embryos which enabled transfer to both uterine horns. Embryos were picked up in a modified capillary tube and transferred through the cervix into each uterine horn of an unanesthetized mouse. A glass speculum was used to facilitate location of the cervix. The technique was found to be as successful (up to 60% of embryos transferred developed to term) as surgical methods yet was simpler and eliminated surgical trauma to the recipient mouse.
D000818:Animals; D004624:Embryo Transfer / Q000295:instrumentation / Q000379:methods*; D005260:Female; D051379:Mice / Q000502:physiology*; D011247:Pregnancy