Microflowfluorometric evaluation of homeostasis in cultured epidermal cells.
In isolating and culturing in vitro populations of basal cells from adult guinea pig skin, it has been possible to show that these cells are sensitive to both G1 and G2 inhibitions. Only a small fraction (10 percent or less) of the G1 blocked cell population would be governed by G1 inhibitory messages released by suprabasal, maturing keratinocytes. As regards the G2 block in vitro experiments confirm that basal cells produce a G2 blocker to which about 9 per cent or less are susceptible. In conclusion basal cells in culture are sensitive to homeostatic regulation as in vivo.
D000818:Animals; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D000078404:Epidermal Cells; D004817:Epidermis / Q000502:physiology; D005470:Fluorometry; D006168:Guinea Pigs; D006706:Homeostasis; D008677:Metaphase / Q000187:drug effects; D008938:Mitosis
D004247:DNA; D003078:Colchicine