title: Comparison of lipid composition of Candida guilliermondii grown on glucose, ethanol and methanol as the sole carbon source.
abstract: The carbon and energy source for aerobically grown cultures of Candida guilliermondii profoundly influenced the neutral lipid content and the fatty acid composition of the individual lipid components. Methanol (0.80%, w/v) grown cells cultivated at 30 C in presence of 0.025% ammonium sulfate contained 12% total lipids, 67% of which was neutral lipids. Glucose (0.74%), w/v) or ethanol (0.53%, w/v) grown cells contained 21--22% total lipids, 80% of which was neutral lipids, under the same conditions. Methanol-grown cells contained a decreased 18:1 acid (52--54% of total fatty acids) and an increased 18:2 acid (23--25%), as compared with glucose- or ethanol-grown cells which contained 57--66% 18:1 acid and 8--14% 18:2 acid, in both neutral and polar lipid fractions. The relationship between methanol metabolism and desaturation of fatty acid in yeast was discussed.
mesh_terms: D002175:Candida / Q000378:metabolism*; D003470:Culture Media; D000431:Ethanol / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005989:Glycerides / Q000378:metabolism; D050356:Lipid Metabolism; D000432:Methanol / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D005989:Glycerides; D010743:Phospholipids; D000431:Ethanol; D005947:Glucose; D000432:Methanol