Investigation of the human macrophage. I. Collection and in vitro cultivation.
By means of blister formation produced by cantharidin, a certain number of human macrophages could be readily obtained from the blister exudate. In the presence of 50% cell-free exudate, these cells were found to proliferate in vitro. The proliferating cells possessed the morphology and functional characteristics of macrophages.
D000818:Animals; D001768:Blister / Q000139:chemically induced; D002193:Cantharidin; D002452:Cell Count; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D002645:Chickens; D006801:Humans; D008264:Macrophages / Q000166:cytology*; D010587:Phagocytosis; D011817:Rabbits; D011961:Receptors, Fc / Q000276:immunology
D011961:Receptors, Fc; D002193:Cantharidin