[Molecular structure of aminopeptidase A from bovine kidney].
Using gel-filtration through Sephadex G-100 and polyacrylamide gel electrophoresis in the presence of 0,5% sodium dodecyl sulfate, it was found that aminopeptidase A has a molecular weight of 65 000 +/- 2000 and is made up of two subunits with mol. weights of 33 000 +/- 2000. Each subunit consists of two polypeptide chains with mol. weights of 22 000 +/- 2000 and 12 000. During enzyme dissociation into subunits the aspartylnaphtylamidase activity is lost, while the glutamylnaphtylamidase activity is retained.
D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D002417:Cattle; D007668:Kidney / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D013379:Substrate Specificity
D046911:Macromolecular Substances; D000626:Aminopeptidases
Molekuliarnaia struktura aminopeptideay A iz pochek byka.