The influence of antigen properties on the conditions required to elute antibodies from immunoadsorbents.
Immunoadsorbents were used to purify a number of antibodies. Using pH 2.0 acid conditions alone it was possible to elute antibodies raised against human growth hormone (HGH) and Fc fragments from such immunoadsorbents with 50% or better recovery of antibody activity. However, to elute antibodies raised against triiodothyronine and cortisol required 6 M guanidine HCl, pH 2.0. The avidities of the purified antibodies were similar to those of the non-purified antibodies. The purified antibodies were stable in solution at 4 degrees C for at least six months.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D000941:Antigens; D013006:Growth Hormone / Q000276:immunology; D006854:Hydrocortisone / Q000276:immunology; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007164:Immunosorbents; D008722:Methods; D010527:Perissodactyla / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D014284:Triiodothyronine / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D007141:Immunoglobulin Fc Fragments; D007164:Immunosorbents; D014284:Triiodothyronine; D013006:Growth Hormone; D006854:Hydrocortisone