Elongation factor Tu-induced conformational changes of ribosomes detected by iodination.
The effect of elongation factor (EF) Tu, bound to the ribosome with the help of poly(uridylic) acid, Phe-tRNA and guanyl-5'-yl methylene diphosphonate, on the conformation and/or chemical environment of ribosomal proteins has been examined using, as a probe, protein iodination. Ribosomes complexed only with poly(uridylic acid) and Phe-tRNA have been used as a control. EF-Tu on the ribosome significantly increases the iodination of proteins S7, S10 and L3 and decreases that of S21 and L18.
D007784:Lactoperoxidase / Q000378:metabolism; D010445:Peptide Elongation Factors / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
D010445:Peptide Elongation Factors; D012269:Ribosomal Proteins; D007784:Lactoperoxidase