Isoelectric focusing in agarose.
We describe here the use of a new agarose for isoelectric focusing of body fluids and tissue proteins. Macromolecular proteins even when significantly greater in size than 1 X 10(6) mol.wt. were easily and rapidly focused.
D000230:Adenocarcinoma / Q000032:analysis; D001202:Ascitic Fluid / Q000032:analysis; D001798:Blood Proteins / Q000302:isolation & purification*; D003110:Colonic Neoplasms / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D011134:Polysaccharides; D012685:Sepharose
D001798:Blood Proteins; D009363:Neoplasm Proteins; D011134:Polysaccharides; D012685:Sepharose