Effects of storage conditions in vitro on the assay of chick liver glycogen.
1. The reproducibility of an enzymic method for assaying chick liver glycogen was found to be +/- 1.6%. 2. Storage of liver at either -21 or -70 degrees C for up to 4 weeks, after freezing in liquid nitrogen, had no significant effect on glycogen concentration. However, the concentration of free glucose increased by about 50% during this time. 3. Storage of liver in ice-cold saline for up to 3 h proved unsatisfactory because of a significant loss of glycogen. 4. There was no significant difference in immediate glycogen concentration in liver tissue that was freeze-clamped, frozen in liquid nitrogen or collected into ice-cold saline. The free glucose concentration was higher, when estimated immediately after, with the latter two methods of collection of liver tissue.
D000818:Animals; D002645:Chickens / Q000378:metabolism*; D005260:Female; D005615:Freezing; D005947:Glucose / Q000378:metabolism; D008112:Liver Glycogen / Q000032:analysis*; D008722:Methods; D014021:Tissue Preservation
D008112:Liver Glycogen; D005947:Glucose