Electron probe microanalysis of elemental composition of mouse cardiac myocytes during post-natal maturation.
Elemental concentrations in the cytoplasm and nucleus of t tests post-natal mouse myocytes were measured at 2, 4, 8, 16, 32 days and in adults using electron probe X-ray microanalysis. Using an analysis of variance test, significant age dependent changes were found in intracellular potassium, sulfur, phosphorus, and chlorine concentrations (mg/kg dry weight), while sodium and magnesium concentrations did not show significant changes. The application of  t tests following linear regression analyses (age versus concentration for each element) did, however, give a significant slope for cytoplasmic sodium, potassium, sulfur, and phosphorus values. The findings correspond closely with an emission spectroscopy-titrimetric study of whole heart ventricle of the same developmental period (Hazelwood and Nichols, '70).
D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D004577:Electron Probe Microanalysis; D005260:Female; D008274:Magnesium / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism
D002712:Chlorides; D010758:Phosphorus; D013455:Sulfur; D012964:Sodium; D008274:Magnesium; D011188:Potassium