Preparation of [3beta-3H] labeled bile acids and bile alcohols.
[3beta-3H]-bile acids and bile alcohols may be useful for metabolic studies in man and animals because the 3-position is invulnerable to bacterial attack. A number of tritium labeled bile acids and bile alcohols were prepared by selective oxidation of the hydroxyl group at carbon-3 followed by reduction with NaBT4. In each case, the bile acids and bile alcohols epimeric at carbon-3 were resolved by analytical and preparative thin-layer chromatography and characterized by gasliquid chromatography. The average yield was 60--65% and specific activities of the final products were in the range of 7.4 x 10(7) dpm/mg.
D001647:Bile Acids and Salts / Q000138:chemical synthesis*; D055598:Chemical Phenomena; D002621:Chemistry; D002777:Cholestanols / Q000138:chemical synthesis*; D007553:Isotope Labeling / Q000379:methods; D014316:Tritium
D001647:Bile Acids and Salts; D002777:Cholestanols; D014316:Tritium