Inhibition of dog renin activity by pepstatin A.
The inhibitory effect of pepstatin A, an isovaleryl pentapeptide isolated from streptmyces, was studied. Pepstatin A inhibited the action of dog renin on homologus substrate. The Ki value was 7.7 X 10(-7) M and the mode of the inhibition was non-competitive. Pestatin A suppressed angiotensin I generation in dog plasma. Dose-dependent hypotensive effect of pepstatin A was observed in rats with sustained increase in blood pressure during continuous infusion of dog renin. It is concluded that pepstatin A inhibits the dog renin activity in vitro and in vivo.
D000803:Angiotensin I / Q000096:biosynthesis; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D009842:Oligopeptides / Q000494:pharmacology*; D010436:Pepstatins / Q000494:pharmacology*; D051381:Rats; D012083:Renin / Q000097:blood; D000092502:Renin Inhibitors
D000803:Angiotensin I; D009842:Oligopeptides; D010436:Pepstatins; D012083:Renin; D000092502:Renin Inhibitors