title: High-performance liquid chromatographic assay of cyclophosphamide in raw material and parenteral dosage forms.
abstract: A simple, specific, high-performance liquid chromatographic method is described for the assay of cyclophosphamide as the raw material and in parenteral dosage forms. The assay involves dilution, addition of an internal standard (methyl 4-aminobenzoate), injection onto a reversed-phase C18 column, and quantitation with a UV detector at 200 nm. Sensitivity is approximately 40 micrograms/ml. Decomposition products elute at the void volume and do not interfere.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003520:Cyclophosphamide / Q000032:analysis*; D004355:Drug Stability; D007263:Infusions, Parenteral; D007275:Injections, Intravenous; D008722:Methods
chemical_list: D003520:Cyclophosphamide