Participation of granulosa cell populations in radiation response of the follicular apparatus in the ovary of mice.
The aim of this paper was to investigate the fates of granulosa cells in three different types of the follicles during 10 hours after single irradiation with 100 resp. 200 R. Using flash labelling with 3H-thymidine, observations of the ovarian anatomical structures during 4--5 estruses after 150 R were made. It has been found, that the basic radiobiological event, influencing further fates of the follicles, is delay of granulosa cell proliferation. Consequently, the further growth and maturation of the follicles is retarded and a reduction of ovulation, a lengthening and an irregularity of estrus take place. The killing effect within granulosa cells is not significant and is of secondary importance.
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D005260:Female; D006107:Granulosa Cells / Q000528:radiation effects*; D051379:Mice; D008815:Mice, Inbred Strains; D006080:Ovarian Follicle / Q000528:radiation effects*; D014965:X-Rays