Structure of the anion-transport protein of the human erythrocyte membrane. Further studies on the fragments produced by proteolytic digestion.
The topology of the human erythrocyte membrane anion-transport protein (band 3) has been investigated by isolation and peptide 'mapping' of the major and minor fragments derived from proteolytic cleavage of the lactoperoxidase 125I-labelled protein in erythrocytes and erythrocyte membranes. The content, in each fragment, of lactoperoxidase 125I-labelled sites (which have a known location in the extracellular or cytoplasmic domain of the protein), together with the location of the sites of proteolytic cleavage yielding the fragments, has allowed us to determine the alignment of the fragments on the linear amino acid sequence and to infer the topology of the polypeptide in the membrane. The results suggest that a region in the C-terminal portion of the polypeptide forms part of the cytoplasmic domain of the protein in addition to a large N-terminal segment. The membrane-bound regions of the protein are located in the C-terminal two-thirds of the molecule. In this region the polypeptide chain traverses the membrane at least four times and an additional loop of polypeptide is either embedded in the membrane or also penetrates through it to the other surface. The location of the lectin receptors on the protein and the site of binding of an anion-transport inhibitor have also been studied.
D000595:Amino Acid Sequence; D000838:Anions; D001798:Blood Proteins; D002268:Carboxypeptidases; D002352:Carrier Proteins / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans; D037102:Lectins; D008565:Membrane Proteins / Q000097:blood*; D008958:Models, Molecular; D010446:Peptide Fragments / Q000097:blood*; D011487:Protein Conformation; D013820:Thermolysin; D014357:Trypsin
D000838:Anions; D001798:Blood Proteins; D002352:Carrier Proteins; D037102:Lectins; D008565:Membrane Proteins; D010446:Peptide Fragments; D002268:Carboxypeptidases; D014357:Trypsin; D013820:Thermolysin