Influence of Mg2+ ions on the activity measurement of isoenzymes of alkaline phosphatase.
The activity of alkaline phosphatase isoenzymes from liver, bone and small intestine is differently influenced by Mg2+. The stimulation of isoenzymes from liver and bone is higher by Mg2+ ions than in the case of isoenzymes from small intestine. An obligatory preincubation of the serum sample in a buffer-Mg2+ mixture is necessary to avoid difficulties which may arise in the kinetic determination of alkaline phosphatase activity under extreme conditions, i.e. low Mg2+ concentration in serum, the necessity of dilution of the sample or the high isoenzyme content from liver or bone in the serum.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D001842:Bone and Bones / Q000201:enzymology; D006801:Humans; D007421:Intestine, Small / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology*
D007527:Isoenzymes; D000469:Alkaline Phosphatase; D008274:Magnesium