A thermostable, sequence-specific restriction endonuclease from Bacillus stearothermophilus: BstPI.
A restriction endonuclease, BstPI, was purified from a strain of B. stearothermophilus, and its cleavage specificity was determined. The enzyme cleaves at palindromic sites of the general structure: (Formula: see text) where N.N' can be any base pair. It produces phosphorylated 5'-termini which are single stranded over a length of 5 nucleotides. Ends generated by cleavage with BstPI can be rejoined by DNA ligase.
D001482:Base Composition; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D013379:Substrate Specificity
D004279:DNA, Viral; D004262:DNA Restriction Enzymes