Structure-function relationship: biological activities of the lipopolysaccharides and lipid A from Vibrio cholerae.
The biological activities of lipopolysaccharides and the lipid A moiety from five different strains of Vibrio cholerae have been investigated. Lipid A was biologically the most active component of lipopolysaccharides from these bacteria. Immunization of rabbits with lipid A from Inaba (66/64) strain led to the production of specific antibodies to lipid A. On fractionation of the immunoglobulins with Sephadex G-200 column chromatography, antibodies against lipid A were detected in the IgM and IgG fractions.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002642:Chick Embryo / Q000187:drug effects; D003169:Complement Inactivator Proteins; D007114:Immunization; D007070:Immunoglobulin A / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008050:Lipid A / Q000494:pharmacology / Q000633:toxicity; D008070:Lipopolysaccharides / Q000494:pharmacology* / Q000633:toxicity; D051379:Mice; D011749:Pyrogens; D013329:Structure-Activity Relationship; D014734:Vibrio cholerae / Q000032:analysis*
D000907:Antibodies, Bacterial; D003169:Complement Inactivator Proteins; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D008050:Lipid A; D008070:Lipopolysaccharides; D011749:Pyrogens