Simultaneous automated determination of free and total sulfisoxazole and sulfamethoxazole in plasma and urine.
A fully automated method for the determination of sulfisoxazole, N4-acetylsulfisoxazole, sulfamethoxazole, and N4-acetylsulfamethoxazole in human plasma and urine was developed. Untreated plasma is analyzed by automation of dialysis, hydrolysis, color development, and quantitation. The method has a sensitivyt limit of 2 microgram/ml of plasma and has been used successfully to determine sulfonamide levels following administration of sulfoxazole and a combination drug product containing sulfamethoxazole and trimethoprim in humans. Samples are processed at the rate of 40 per hour, with a minimum of sample handling, data reduction, and materials.
D001322:Autoanalysis; D003956:Dialysis; D006801:Humans; D006868:Hydrolysis; D008722:Methods; D013420:Sulfamethoxazole / Q000032:analysis* / Q000097:blood / Q000652:urine; D013444:Sulfisoxazole / Q000032:analysis* / Q000097:blood / Q000652:urine
D013444:Sulfisoxazole; D013420:Sulfamethoxazole