Studies on the high-mobility-group non-histone proteins from hen oviduct.
Nuclear high-mobility-group (HMG) proteins were isolated from hen oviduct. These were proteins HMG-1, -2, -3, -14 and -17, which are equivalent to the classification of calf thymus HMG proteins. Hen oviduct proteins HMG-1 and -2 were individually isolated by HCIO4.extraction and CM-Sephadex chromatographic separation. Their mol.wts. were determined as 28 000 and 27 000, respectively. The proteins have a high content of acidic and basic amino acids. The association of proteins HMG-1 and -2 with the genome of hen oviduct nuclei was probed by a limited digestion with nucleases. Hen oviduct nuclei were incubated with deoxyribonuclease I or micrococcal nuclease until 10% of the DNA was digested. The nuclear suspension was centrifuged and the contents of proteins HMG-1 and -2 in the supernatant and sediment fractions were analysed by polyacrylamide-gel electrophoresis. HMG proteins were found to be preferentially released by micrococcal-nuclease digestion rather than by deoxyribonuclease I.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000032:analysis; D002645:Chickens; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification; D003851:Deoxyribonucleases; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008836:Micrococcal Nuclease; D010057:Oviducts / Q000032:analysis*; D013950:Thymus Gland / Q000032:analysis
D000596:Amino Acids; D002868:Chromosomal Proteins, Non-Histone; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease