Inhibition of growth of ASL-1w murine leukemia cells by anti-thymus leukemia antigen (TL) serum in the absence of complement.
Previous studies have shown that the cell surface expression of thymus leukemia antigen (TL) on ASL-1w leukemia cells varies with the progression of the cells through the growth cycle. Expression of TL is maximal in S phase, and its quantitative expression varies directly with DNA synthesis. In the present study, the effect of anti-TL serum on the growth of ASL-1w cells was examined. The antiserum, tested in the absence of complement, affected the growth of these cells in biphasic manner. When the antiserum concentration was 0.1% or greater, there was a rapid decline in the rate of DNA synthesis, and after 5 to 7 hr, cell death. When the antiserum concentration was 0.067% or less, the decline in the rate of synthesis of DNA did not become apparent until 5 to 6 hr after treatment. Under these conditions, there was approximately a 20% increase in cell number after 24 hr of culture. The hypothesis that treatment of ASL-1w cells with the lesser concentration of anti-TL serum blocks the cells in G2 phase of the cell cycle is discussed.
D000818:Animals; D000951:Antigens, Neoplasm; D002471:Cell Transformation, Neoplastic; D003165:Complement System Proteins / Q000276:immunology*; D004247:DNA / Q000096:biosynthesis; D003703:Demecolcine / Q000494:pharmacology; D006168:Guinea Pigs; D007106:Immune Sera / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000276:immunology*; D051379:Mice; D008938:Mitosis; D013950:Thymus Gland / Q000276:immunology*
D000951:Antigens, Neoplasm; D007106:Immune Sera; D003165:Complement System Proteins; D004247:DNA; D003703:Demecolcine