Intrarenal prostaglandins: effect of sodium and indomethacin on PGE2 and PGF2 alpha in rabbits.
The levels of prostaglandins (PGs) E2 and F2 alpha in different parts of the rabbit kidney were determined to observe the effect of sodium and indomethacin. After the pretreatment with injections of saline or with indomethacin, tissues from inner and outer medulla and cortex were separated, extracted, analyzed for PGE2 and PGF2 alpha by radioimmunoassay. In the normal rabbit kidney, the greatest amount of PG was found in the inner medulla. Saline injections appeared to increase PGE2 (but not PGF2 alpha), especially in the inner medulla. Repeated injections of saline, on the other hand, markedly reduced PGE2 in the inner medulla but increased outer medullary PGE2. Indomethacin reduced the production of PGs in all kidney segments. These results suggest the bidirectional effect of sodium on PG concentration in the rabbit kidney. Acute administration of sodium may directly stimulate the synthesis of PGE2 in the inner medulla but chronic stimulation with sodium may alter the pattern of PGE2 synthesis.
D000818:Animals; D007213:Indomethacin / Q000494:pharmacology*; D007668:Kidney / Q000032:analysis* / Q000187:drug effects; D007672:Kidney Cortex / Q000032:analysis; D007679:Kidney Medulla / Q000032:analysis; D008297:Male; D011453:Prostaglandins / Q000032:analysis*; D011458:Prostaglandins E / Q000032:analysis; D011460:Prostaglandins F / Q000032:analysis; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology*
D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D012964:Sodium; D007213:Indomethacin