Isatin inhibition of rat testicular alkaline phosphatase. A non-allosteric phenomenon.
Isatin has been found to inhibit rat testicular alkaline phosphatase (EC 3.1.3.1) uncompetitively. The hyperbolic curve relating inhibition as a function of substrate concentration; the persistence of inhibition after the tertiary structure of the enzyme has been altered by heat denaturation, exposure to urea or papain digestion; the small changes in entropy, free energy and enthalpy in the presence of isatin, and the number of isatin molecules (n = 1.29) combining with one molecule of enzyme indicate the non-allosteric nature of inhibition.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000495:Allosteric Site; D000818:Animals; D007211:Indoles / Q000494:pharmacology*; D007510:Isatin / Q000494:pharmacology*; D008297:Male; D011487:Protein Conformation; D051381:Rats; D013737:Testis / Q000201:enzymology*; D013816:Thermodynamics
D007211:Indoles; D007510:Isatin; D000469:Alkaline Phosphatase