Decreased heparin sensitivity of cyclohexanedione-modified factor Xa.
Modification of 12 arginine residues of bovine Factor Xa with 1,2-cyclohexanedione has resulted in a decrease in heparin sensitivity of the reaction between enzyme and antithrombin-III, whereas the antithrombin-III sensitivity of modified Factor Xa was only slightly affected. It is suggested that heparin accelerates the Factor Xa-antithrombin-III reaction by interacting with Factor Xa.
D000818:Animals; D000990:Antithrombin III / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D002417:Cattle; D003510:Cyclohexanes / Q000494:pharmacology*; D003512:Cyclohexanones / Q000494:pharmacology*; D005170:Factor X / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics
D003510:Cyclohexanes; D003512:Cyclohexanones; C018031:1,2-cyclohexanedione; D000990:Antithrombin III; D005170:Factor X; D006493:Heparin