[A method for production of Coxiella burnetii antigen and cell walls by guanidiniumchloride extraction (author's transl)].
By use of 6-molar guanidinium chloride a potent Coxiella burnetii antigen could be produced for diagnostic purposes from infected yolk sacs, with little technical expense. This treatment did not only a cause remarkably purifying effect (Tab. 1) but also the extraction of soluble cytoplasmic substance. From guanidinium chloride-treated suspensions a highly purified and uniform suspension of cell walls could be separated by Saccharose Density Gradient centrifugation (Fig. 2). Guanidine extracted organisms retained their full antigenic potential with respect to Phase I and Phase II and lacked anticomplementary activity. Such preparations can be used for serological tests like complement fixation reaction or Enzyme Linked Immunosorbent Assay and are particularly suitable for biochemical studies of Phase antigens of Coxiella burnetii.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002473:Cell Wall / Q000276:immunology*; D002712:Chlorides; D003381:Coxiella / Q000276:immunology* / Q000648:ultrastructure; D006146:Guanidines; D008722:Methods
D000942:Antigens, Bacterial; D002712:Chlorides; D006146:Guanidines
Eine Methode zur Gewinnung von Antigen bzw. Zellwänden von Coxiella burnetii mittels Guanidiniumchlorid-Extraktion.