Growth and G1 arrest of sarcoma virus trnasformed cells in serum free media.
Rous-sarcoma transformed BHK cells can be continuously cultured in a medium containing Eagle's Minimal Essential Medium, iron and biotin. The rate of cell multiplication increased when serine, or serine plus other non-essential amino-acids were added to the medium. With biotin deleted from the medium there is a reduction in DNA synthesis and most cells are blocked in G1.
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001710:Biotin / Q000494:pharmacology; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D007399:Interphase; D007501:Iron / Q000494:pharmacology; D007668:Kidney
D000596:Amino Acids; D003470:Culture Media; D001710:Biotin; D004247:DNA; D007501:Iron