The anti-proteolytic behavior of lathyrogens.
The lathyrogens, beta-aminopropionitrile and alpha-aminoacetonitrile inhibit both the esterolytic and proteolytic activity of trypsin at a concentration of 100 mM. Lineweaver-Burk plots demonstrate that inhibition is competitive, with alpha-aminoacetonitrile being the more potent inhibitor. The enzyme associated with and capable of digesting tropoelastin is inhibited by both lathyrogens when tested against its natural substrate, tropoelastin. Administration of alpha-aminoacetonitrile-HCl to the diet of young chicks (0.1% w/w) resulted in a 62% increase in the yield of tropoelastin and significant reduction in fatality as compared to beta-aminopropionitrile-fumarate.
D000097:Acetonitriles / Q000494:pharmacology*; D000607:Aminoacetonitrile / Q000494:pharmacology*; D000629:Aminopropionitrile / Q000494:pharmacology*; D000818:Animals; D001586:Benzoylarginine Nitroanilide / Q000037:antagonists & inhibitors; D001667:Binding, Competitive; D002642:Chick Embryo; D002645:Chickens; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D004549:Elastin / Q000031:analogs & derivatives*; D005650:Fumarates / Q000494:pharmacology; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D011480:Protease Inhibitors; D014333:Tropoelastin / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000494:pharmacology*
D000097:Acetonitriles; D005650:Fumarates; D011480:Protease Inhibitors; D014333:Tropoelastin; D014361:Trypsin Inhibitors; D000629:Aminopropionitrile; D000607:Aminoacetonitrile; D004549:Elastin; D001586:Benzoylarginine Nitroanilide; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin