Isolation of the fourth component (C4) of rat complement.
The fourth component of rat complement was purified to homogeneity by sequential chromatography of rat plasma in benzamidine on QAE-A50, SP-C50, hydroxyapatite, and gel filtration on Bio-Gel A 1.5. The final material was homogeneous on SDS-PAGE analysis and had a calculated m.w. of 198,000. A monospecific antibody against rat C4 was obtained from immunized rabbits. The concentration of rat C4 in the plasma of normal 4-month-old Wistar rats was 190 +/- 34 microgram/ml (mean +/- 1 S.D.).
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003181:Complement C4 / Q000276:immunology / Q000302:isolation & purification*; D006168:Guinea Pigs; D006882:Hydroxyapatites; D007106:Immune Sera / Q000494:pharmacology; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats
D003181:Complement C4; D006882:Hydroxyapatites; D007106:Immune Sera