Radioassays of blood group M, N and T (Thomsen-Friedenreich) antigens.
Radioassays employing the double-antibody or Farr techniques were developed for the M, N and T antigens. Blood group glycoproteins were isolated by butanol extraction of red cell stroma and iodinated by the chloramine-T technique. The final purity of glycoprotein was over 75% as judged by radioimmunoassay (RIA). T activation of glycoprotein was obtained with neuraminidase. A specific RIA was obtained for the M antigen and was sensitive to approximately 10 ng of glycoprotein or glycopeptide. In the RIA system rabbit anti-M displayed a higher affinity for M glycoprotein than for M glycopeptide. A RIA that was entirely specific for the N antigen, could not be obtained. A radioassay, obtained for the T antigen with peanut agglutinin in the Farr technique, was sensitive to approximately 100 ng of T antigen and was readily inhibitable by monosaccharides. A RIA, obtained for the T antigen with rabbit anti-T, was entirely specific and sensitive to approximately 1 ng of T activated glycoprotein or glycopeptide but was not inhibitable by monosaccharides.
D001789:Blood Group Antigens / Q000276:immunology*; D006020:Glycopeptides / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D037102:Lectins / Q000276:immunology; D008951:MNSs Blood-Group System / Q000276:immunology; D009005:Monosaccharides / Q000494:pharmacology; D009439:Neuraminidase / Q000494:pharmacology; D011863:Radioimmunoassay / Q000379:methods
D001789:Blood Group Antigens; D006020:Glycopeptides; D006023:Glycoproteins; D037102:Lectins; D008951:MNSs Blood-Group System; D009005:Monosaccharides; D009439:Neuraminidase