p-Aminohippuric acid transport into brush border vesicles isolated from flounder kidney.
p-Aminohippuric acid (PAH) transport was investigated in brush border vesicles isolated from renal proximal tubules of the winter flounder. Three characteristics of carrier-mediated transport were demonstrated: 1) unlabeled PAH inhibited the uptake of [3H]PAH; 2)[3H]PAH efflux from the vesicles was stimulated in the presence of unlabeled PAH in the extravesicular medium; and 3) PAH influx was inhibited by 2,4-dinitrophenol (DNP) and 4-acetamido-4'-isothiocyano-2,2'-disulfonic stilbene (SITS). D-Glucose plus a sodium gradient stimulated PAH uptake, as did a K2SO4 gradient plus valinomycin, suggesting that PAH is transported as an anion. In contrast, PAH uptake into a membrane fraction containing mainly basal-lateral plasma membranes exhibited a larger inhibition by probenecid but a smaller inhibition by unlabeled PAH and SITS. Thus, carrier-mediated transfer of PAH driven by the electrochemical potential difference for PAH is demonstrated in the brush border membrane of the flounder kidney.
D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D004140:Dinitrophenols / Q000494:pharmacology; D005399:Fishes / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism* / Q000502:physiology; D008564:Membrane Potentials; D008871:Microvilli / Q000378:metabolism*; D010130:p-Aminohippuric Acid / Q000378:metabolism* / Q000494:pharmacology
D000618:Aminohippuric Acids; D004140:Dinitrophenols; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D010130:p-Aminohippuric Acid