[Comparative analysis of cytosolic and solubilized mitochondrial menadione reductases from rat liver].
The kinetic properties of cytosolic and solubilized mitochondrial menadione reductases (EC 1.6.99.2) from rat liver were compared. The mechanism of the reaction of cytosolic and mitochondrial menadione reductases with NADH and 4-anilino-5-methoxy-1,2-benzoquinone (AMOBQ) as substrates obeys the "ping-pong" kinetics. AMOBQ is a competitive inhibitor of cytosolic menadione reductase (Ki = 219 microM). Both menadione reductases have similar or identical values of true and effective kinetic constants and similar electrophoretic mobilities.
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D011808:Quinone Reductases / Q000378:metabolism*; D051381:Rats
D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases
Sravnitel'noe izuchenie tsitozol'noĭ i soliubilizirovannoĭ mitokhondrial'noĭ menadionreduktaz iz pecheni krysy.