Enzymatic preparation of adenosine 5'-O-(3-[35S]thiotriphosphate) by thiophosphorylation of adenosine diphosphate.
The very useful radiolabeled ATP analog, adenosine 5-O-(3-[35S] thiotriphosphate) or [35S] ATPgammaS, has been prepared by a technique based on the thiophosphorylation of ADP that results in much higher yields of [35S] ATPgammaS than does a thiophosphate exchange method [1].
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D005986:Glyceraldehyde 3-Phosphate; D008722:Methods; D010766:Phosphorylation; D010770:Phosphotransferases; D013462:Sulfur Radioisotopes; D013873:Thionucleotides / Q000138:chemical synthesis*
D013462:Sulfur Radioisotopes; D013873:Thionucleotides; D005986:Glyceraldehyde 3-Phosphate; C022571:adenosine 5'-O-(3-thiotriphosphate); D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases