Cytochemical and ultrastructural alteration of leukemic cells by a naturally cytotoxic factor from spleen.
Experiments were carried out to delineate the differences in cytocidal modality of natural splenic cytotoxic factor (30,000-50,000 daltons) and cyto-A against normal and human leukemic lymphocytes in vitro. It was found that the differences in the cytocidal action effected by cyto-A and splenic isolate were as follows: (1) the killing effect of cyto-A against leukemic cells was very rapid in contrast to the slowly acting spleen factor as evaluated by cytochemical and ultrastructural studies; (2) spleen factor was not cytotoxic against normal lymphocytes in contrast to the indiscriminatory cytotoxic cyto-A.
D002471:Cell Transformation, Neoplastic / Q000648:ultrastructure*; D003561:Cytarabine / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D006651:Histocytochemistry; D006801:Humans; D007668:Kidney / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012313:RNA / Q000378:metabolism; D013154:Spleen / Q000276:immunology*; D014020:Tissue Extracts / Q000494:pharmacology*
D010835:Phytohemagglutinins; D014020:Tissue Extracts; D003561:Cytarabine; D012313:RNA