title: Chemotaxigenesis by cell surface components of Staphylococcus aureus.
abstract: In an attempt to delineate the staphylococcal cell surface components of importance in chemotaxigenesis, we incubated intact Staphylococcus aureus H, crude cell walls, purified cell walls, peptidoglycan, teichoic acid, and cell membranes with human sera. The results reported indicate that both crude cell walls and purified cell walls, as well as peptidoglycan, were potent chemotaxigens. These particles led to the generation in normal human serum of a factor that was chemotactic for human polymorphonuclear leukocytes. Cell wall peptidoglycan and teichoic acid both appeared to play a role in chemotaxigenesis. Kinetic studies employing C2-deficient serum and immunoglobulin-deficient serum revealed that optimal chemotaxigenesis required the presence of an intact classical complement pathway, as well as antibody. Granulocyte aggregometry studies showed that significant levels of C5a were generated in normal serum and that this activated complement component appears to be a major chemotactic factor produced in serum upon interaction with staphylococcal cell wall components.
mesh_terms: D002462:Cell Membrane / Q000502:physiology; D002473:Cell Wall / Q000502:physiology; D002634:Chemotaxis, Leukocyte; D003182:Complement C5 / Q000502:physiology; D003171:Complement Pathway, Classical; D006801:Humans; D009504:Neutrophils; D010457:Peptidoglycan / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000502:physiology* / Q000648:ultrastructure; D013682:Teichoic Acids / Q000494:pharmacology
chemical_list: D003182:Complement C5; D010457:Peptidoglycan; D013682:Teichoic Acids