Investigations on the lectin-producing cells in the sponge Axinella polypoides (Schmidt).
The localization of two carohydrate binding proteins, so-called lectins, was studied in the sponge tissue of Axinella polypoides by light and immunofluorescence microscopy. They do not occur at the cellular surface of any cell type, but they are stored in vesicles of the "spherulous cells". After short formaldehyde fixation spherulous cells can be isolated and they release the active lectins upon lysis in distilled water. Electron microscopical studies of spherulous cells show that they contain almost nothing else but a small nucleus and vesicles of different size and number. Small vesicles are full of an electron dense material, whereas the content of large vesicles has a fluffy and fibrillar structure. Spherulous cells are large and tightly packed in the outer layer of the ectosome and in the meshwork of the spongin fibres of the central axis. The are small and scattered in the inner layer of the ectosome, and they are found throughout the choanosome. The function of the lectins is not clearly defined, and different alternatives such as participation in glycoprotein synthesis, immunological defense, or carbohydrate transport are possible.
D000818:Animals; D002469:Cell Separation; D006651:Histocytochemistry; D037102:Lectins; D008854:Microscopy, Electron; D011161:Porifera / Q000166:cytology / Q000378:metabolism*
D037102:Lectins