A thin-layer chromatographic assay for measuring pineal hydroxyindole-O-methyltransferase activity.
A thin-layer chromatographic procedure for measuring pineal HIOMT activity is described, based on the methylation of NAS to melatonin. The method enables simple and accurate determination in small aliquots from a single pineal organ homogenate.
D000121:Acetylserotonin O-Methyltransferase / Q000032:analysis*; D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods; D007700:Kinetics; D008297:Male; D008780:Methyltransferases / Q000032:analysis*; D010870:Pineal Gland / Q000201:enzymology*; D051381:Rats
D008780:Methyltransferases; D000121:Acetylserotonin O-Methyltransferase