Enzymatic hydrolysis of leucylaminoantipyrine in various tissues and subcellular fractions of rat liver and kidney.
Enzymatic hydrolysis of L-leucylaminoantipyrine occurs in homogenates of kidney, small intestine, liver, lung, brain, spleen, heart and blood plasma. The activity of the drug hydrolyzing enzyme was found in all tested subcellular fractions of liver and kidney. In contrast to L-leucylaminoantipyrine, neither its stereoisomer D-nor 4-L-(N,N-dimethylleucylamino)-antipyrine undergoes enzymatic hydrolysis in any tissue tested. The results suggest, that in vivo L-leucylaminoantipyrine could be very rapidly hydrolyzed to 4-aminoantipyrine.
D000632:Aminopyrine / Q000031:analogs & derivatives*; D000818:Animals; D006868:Hydrolysis; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000031:analogs & derivatives; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism
D000632:Aminopyrine; D007930:Leucine