[Reversible precipitation of the glucocorticoid receptor from rat liver by La3+ ions].
It was shown that La3+ ions are capable of precipitating the glucocorticoid receptor from rat liver. Treatment of cytosol containing either free receptor or the hormonereceptor complex with 0,005 M La(NO3)3 results in recptor precipitation. The pellet is readily dissolved in buffer with EDTA. Transcortin and transcortin-like proteins are not affected by La3+ ions. The lanthanium-treated receptor does not lose its ability to bind DNA and chromatin in vitro. It is suggested that precipitation by La3+ ions can be used for separation of the receptor from transcortin and transcortin-like proteins as well as for evaluation of binding parameters for steroid-receptor complexes in rat liver cytosol.
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D007700:Kinetics; D007811:Lanthanum; D008099:Liver / Q000378:metabolism*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification* / Q000378:metabolism; D011987:Receptors, Steroid / Q000302:isolation & purification*; D014156:Transcortin / Q000302:isolation & purification
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D007811:Lanthanum; D014156:Transcortin
Obratimoe osazhdenie retseptora gliukokortikoidnykh gormonov ionami La3+.