Selection of a high performance liquid chromatographic cleanup procedure for the determination of organochlorine pesticides in fatty biological extracts.
Two different high performance liquid chromatographic cleanup methods, absorption chromatography and nonaqueous reversed-phase chromatography, were investigated and their respective adventages discussed. The first was performed on 5 cm silica and alumina columsn, while for the latter a 5 cm octadecylilica column was used. Optimum mobile phase moderator concentrations were selected in order to achieve complete separation between the most retained organochlorine pesticide (endosulfan sulfate) and lipids on absorption columns. Separation efficiency was controlled by monitoring the eluate at 205 nm. The specific application fields of the described approaches were indicated and their possibility to preclude extracts from up to mg of lipid substances proved.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D008722:Methods
D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D008055:Lipids