Ion cluster techniques in drug metabolism: an example of the use of mixtures of the [12C]:[14C] isotopic forms of the synthetic prostaglandin cloprostenol ('Estrumate') to facilitate metabolite identification.
The synthetic prostaglandin analogue cloprostenol has been prepared radiolabelled with 14C. The isotopic abundance of 14C at position C-15 was greater than 90% of the theoretical maximum. We have utilizted the high abundance of the 14C isotope for metabolism studies by preparing mixtures of [12C]:[14C]cloprostenol such that fragments detected by mass spectrometry contained characteristic isotope clusters analogous to those often obtained using stable isotopes.
D000818:Animals; D002250:Carbon Radioisotopes; D003008:Cloprostenol / Q000378:metabolism / Q000652:urine*; D005260:Female; D013058:Mass Spectrometry / Q000379:methods*; D011461:Prostaglandins F, Synthetic / Q000652:urine*; D051381:Rats; D013678:Technology, Pharmaceutical; D014297:Trimethylsilyl Compounds
D002250:Carbon Radioisotopes; D011461:Prostaglandins F, Synthetic; D014297:Trimethylsilyl Compounds; D003008:Cloprostenol