Cholesterol biosynthesis and progesterone production by chorionic cells of the early sheep conceptus in vitro.
Dissociated trophoblast cells from the chorion of the 21-day sheep conceptus were shown to produce progesterone in vitro. Evidence that this represented synthesis de novo was provided by the prolonged production of progesterone in the absence of added precursor steroids. Radioactive cholesterol was isolated after incubation of dispersed chorionic cells with acetate-1-14C.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis*; D002823:Chorion / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D011247:Pregnancy; D011374:Progesterone / Q000096:biosynthesis*; D012756:Sheep / Q000378:metabolism
D000085:Acetates; D011374:Progesterone; D002784:Cholesterol