Structural requirements for the inhibition of phthalate ester hydrolysis in rainbow trout by methylenedioxyphenyl compounds.
1. The ability of a number of methylenedioxyphenyl compounds and paraoxon to inhibit the hydrolysis and oxidation of di-2-ethylhexyl phthalate by rainbow trout tissue preparations was examined. 2. In addition to paraoxon, only the methylenedioxyphenyl compounds with long side-chains (piperonyl butoxide and tropital) inhibited the hydrolysis of di-2-ethylhexyl phthalate by trout liver subcellular fractions and trout serum. 3. Paraoxon decreased the production of the major metabolite of di-2-ethylhexyl phthalate by trout liver microsomes (+ NADPH) suggesting that this metabolite arises via further metabolism of mono-2-ethylhexyl phthalate.
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D003864:Depression, Chemical; D004051:Diethylhexyl Phthalate / Q000378:metabolism*; D004148:Dioxolanes / Q000494:pharmacology*; D004149:Dioxoles / Q000494:pharmacology*; D006868:Hydrolysis; D008099:Liver / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D010795:Phthalic Acids / Q000378:metabolism*; D012487:Salmonidae / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014337:Trout / Q000378:metabolism*
D004148:Dioxolanes; D004149:Dioxoles; D010795:Phthalic Acids; D004051:Diethylhexyl Phthalate