Isolation of wheat ribosomes free of high molecular weight inhibitors of the natural messenger translation.
The cell-free extract from wheat germ contains an inhibitor interfering with translation of a natural template (BMV RNA). The inhibitor affects neither the translation of poly(U) nor the aminoacylation of tRNA. It exhibits the activity of protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37). The inhibitor is found in lipoprotein aggregates which can be separated from ribosomes on Sepharose 2B column. Ribosomes purified on the Sepharose are several times more active in translation of BMV RNA than those isolated by conventional methods.
D002850:Chromatography, Gel / Q000379:methods; D009029:Mosaic Viruses / Q000235:genetics; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral; D012270:Ribosomes / Q000032:analysis*; D014908:Triticum; D014461:Ultracentrifugation / Q000379:methods
D012333:RNA, Messenger; D012367:RNA, Viral; D011494:Protein Kinases