Effects of various media on tissular and cellular structures of the superior cervical ganglion of the rat.
The superior cervical ganglia of the rat have been incubated in vitro for 1 h in basal medium Eagle (BME) with Hanks' salts, BME with Earle's salts, Kreb's solution and NCTC 109 medium. Comparison of the cell areas, established by a semi-automatic quantitative method, shows that the three former induce a 30--35% neuronal retraction, whereas NCTC 109 has no effect. Thus this latter medium seems the best one for studies using incubation of these cells.
D000818:Animals; D003470:Culture Media; D005728:Ganglia, Sympathetic / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D010477:Perfusion; D051381:Rats
D003470:Culture Media