title: Growth of measles virus in continuous cell lines derived from the nervous tissues of human and rat.
abstract: Growth of two measles virus strains, the TYCSA and CAM, was compared in three continuous cell lines derived from the nervous tissues, human neuroblastoma IMR-32, human glioma 118MGC, and rat glioma C-6. The two human neural cells were shown to support the growth of both measles virus strains as efficiently as in the non-neural Vero cells. Different types of cytopathic effect (CPE) between the two virus strains were noticed in IMR-32 cells; the CAM strain induced strand-forming type CPE and the TYCSA strain giant-cell type CPE. As a difference of growth pattern between IMR-32 and 118MGC cells, virus antigen was demonstrated in both the nucleus and cytoplasm of 118MGC cells whereas virus antigen was present only in the cytoplasm of IMR-32 cells. In contrast to the productive infection in human neural cells, growth of both virus strains was restricted in rat glioma C-6 cells without showing CPE although the prolonged presence of virus antigens was demonstrated by the immunofluorescent technique.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001254:Astrocytoma; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D005910:Glioma; D006801:Humans; D007181:Inclusion Bodies, Viral; D008459:Measles virus / Q000254:growth & development* / Q000276:immunology; D009447:Neuroblastoma; D051381:Rats; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral