Role of oxygen radicals scavenging enzymes in the protoporphyrin induced photohemolysis.
Inhibition of superoxide dismutase by diethyldithiocarbamate or cyanide increases the rate of red blood cells lysis after irradiation in the presence of protoporphyrin IX. Catalase activity, which is decreased during the photohemolytic process, appears to be not essential for the lytic event. No relationship between catalase activity and hemolysis rate was found. Superoxide dismutase appears to prevent only in part catalase inactivation by singlet oxygen.
D000818:Animals; D001386:Azides / Q000494:pharmacology; D002417:Cattle; D003486:Cyanides / Q000494:pharmacology; D004285:Dogs; D004912:Erythrocytes / Q000187:drug effects / Q000528:radiation effects*; D006461:Hemolysis / Q000187:drug effects / Q000528:radiation effects*; D006736:Horses; D006801:Humans; D008027:Light; D051379:Mice; D011166:Porphyrins / Q000494:pharmacology*; D011524:Protoporphyrins / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D001386:Azides; D003486:Cyanides; D011166:Porphyrins; D011524:Protoporphyrins