Measurement of arachidonic acid in the plasma by gas-liquid chromatography-flame ionization using dihomo-gamma-linolenic acid as an internal standard.
A gas-liquid chromatography-flame ionization method is described for measuring arachidonic acid in plasma using dihomo-gamma-linolenic acid as an internal standard. We found this technique to -e reproducible, and quicker and superior to previously described techniques because of the similar physico-chemical properties of the unsaturated fatty acid internal standard and arachidonic acid. In addition, we observed that the use of the saturated fatty acid, n-tricosanoic acid, was unsatisfactory as an internal standard because of its poor extractability from plasma as compared to arachidonic acid.
D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives; D001095:Arachidonic Acids / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans
D001095:Arachidonic Acids; D015126:8,11,14-Eicosatrienoic Acid