[Effect of cytosol on RNA-synthesizing ability of isolated nuclei of rat hepatocytes].
The addition of cytozol to isolated nuclei of the liver is accompanied by a rise in the amount of a new synthetizing RNA and changes in its nucleotide composition. An increase in the intensity of [3H] ATP incorporation at the end of incubation evidences for polyadenylation of the reaction product. Under the effect of cytozol 30-32% of RNA synthetized de novo is released. Heating of cytozol (50 degrees C, 15 min) leads to a loss of its ability to activate RNA synthesis by isolated nuclei.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*
D012313:RNA; D000255:Adenosine Triphosphate
Vliianie tsitozola na RNK-sinteziruiushchuiu sposobnost' izolirovannykh iader gepatotsitov krys.