Effects of low temperatures on survival of frozen-thawed mouse embryos.
Almost no damage to mouse morulae was observed between 0 and -40 degrees C, and freezing damage to embryos in DMSO, glycerol or ethylene glycol occurred after exposure to -60, -50 or -50 degrees C, respectively. Cooling embryos in DMSO or glycerol to temperatures below -50 to -60 degrees C increased freezing damage. To the contrary, in the presence of ethylene glycol, no more damage occurred after exposure to temperatures below -50 degrees C.
D000818:Animals; D004121:Dimethyl Sulfoxide; D004622:Embryo, Mammalian / Q000502:physiology*; D005026:Ethylene Glycols; D005260:Female; D005615:Freezing; D005990:Glycerol; D051379:Mice; D011247:Pregnancy; D013696:Temperature
D005026:Ethylene Glycols; D005990:Glycerol; D004121:Dimethyl Sulfoxide