Cleavage of the taurine conjugate of 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestan-26-oic acid by rat fecal bacteria.
This paper describes a method for the hydrolysis of the taurine conjugates of the 25R and the 25S diastereoisomers of 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestan-26-oic acid (THCA) with retention of original configuration of C-25. Rat fecal suspensions were incubated with the taurine conjugate of THCA for 5 and 60% of the free THCA was recovered. When bile from Alligator mississippiensis, which contains mostly the taurine conjugate of THCA, was analyzed by this method, THCA was obtained with the 25R configuration.
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D002777:Cholestanols / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D007700:Kinetics; D051381:Rats; D013237:Stereoisomerism; D013654:Taurine / Q000378:metabolism*
D002777:Cholestanols; D013654:Taurine