DNA degradation in isolated rat liver nuclei.
Purified rat liver nuclei were incubated at 4 degrees C. After different intervals of incubation aliquots of the nuclear suspension were taken and DNA was extracted by a common SDS-phenol-chloroform procedure. The fractionation of DNA by agar gel electrophoresis revealed large DNA fragments. It was shown that the well-known DNA degradation to monomers and its multiples is preceeded by an earlier breakdown of DNA into characteristic large fragments.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D003080:Cold Temperature; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D008099:Liver / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats
D004247:DNA