Mutagenicity of metal cations in cultured cells from Chinese hamster.
The mutations at the hypoxanthine-guanine phosphoribosyl transferase (HGPRTase) locus in Chinese hamster V79 cells induced by metal cations were examined by the development of resistance to 8-azaguanine (8AG). The spontaneous frequency of 8AG resistance was 5.8 per 10(6) cells, and the frequency was enhanced to 2-6 times that of the control by treatment of cells with the chlorides of beryllium and manganese. About 75% of 8AG-resistant colonies were sensitive to amethopterin, and 86% of the resistant colonies showed less than 3% of the HGPRTase activity of the wild-type cells. The mutant frequency in cultures treated with cobalt and nickel chlorides were slightly increased, and mutation induction was only detectable at very low rates of cell survival.
D000818:Animals; D001375:Azaguanine / Q000494:pharmacology*; D001608:Beryllium / Q000494:pharmacology; D002460:Cell Line; D002712:Chlorides / Q000494:pharmacology; D003035:Cobalt / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D007041:Hypoxanthine Phosphoribosyltransferase / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects*; D009532:Nickel / Q000494:pharmacology
D002712:Chlorides; D008670:Metals; D008769:Methylnitronitrosoguanidine; D003035:Cobalt; D008345:Manganese; D009532:Nickel; D007041:Hypoxanthine Phosphoribosyltransferase; D001608:Beryllium; D001375:Azaguanine