Quantitative measurement of intestinal marker enzymes in intestinal metaplasia from human stomach with cancer.
Intestinal metaplasia in human stomach was distinguished macroscopically into sucrase-positive and trehalase-positive areas, and sucrase-positive and trehalase-negative areas, by location of these disaccharidase activities with TES-Tape. After location of these two areas with TES-Tape, tissues were taken from them for colorimetric measurement of sucrase, trehalase, leucine aminopeptidase (LAP), and alkaline phosphatase (ALP). Results showed that in the mucosa from sucrase-positive and trehalase-negative areas, trehalase activity was not detectable and the activities of sucrase, LAP, and ALP were lower than in sucrase-positive and trehalase-positive areas.
D000469:Alkaline Phosphatase / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D004386:Duodenum / Q000201:enzymology; D005753:Gastric Mucosa / Q000201:enzymology / Q000473:pathology; D006801:Humans; D007931:Leucyl Aminopeptidase / Q000032:analysis*; D008679:Metaplasia / Q000201:enzymology; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology; D013393:Sucrase / Q000032:analysis*; D014198:Trehalase / Q000032:analysis*
D000469:Alkaline Phosphatase; D014198:Trehalase; D013393:Sucrase; D007931:Leucyl Aminopeptidase