Glutamate decarboxylase activity in chick brain and retina. Inhibition of the immature enzyme by Triton-X-100.
We have studied the effect of Triton-X-100 on glutamate decarboxylase (GAD) activity in brain and retina from chick embryos of 12 and 16 days' incubation and from chicks 4--6 weeks old. GAD activity was measured in five different homogenization media. Triton-X-100 inhibited the enzyme by about 60% in both brain and retina of 12-day embryos and by about 50% in 16-day embryos, independently of the homogenization medium. In chicks only about 20% inhibition by the detergent was observed in brain whereas no effect was found in retina. These results indicate that the evaluation of the experimental conditions of enzyme assays at different ages is essential for developmental studies of GAD activity in nervous tissue.
D000375:Aging; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D002642:Chick Embryo; D002645:Chickens / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000037:antagonists & inhibitors*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012160:Retina / Q000187:drug effects / Q000201:enzymology*
D011092:Polyethylene Glycols; D002262:Carboxy-Lyases; D005968:Glutamate Decarboxylase