A simplified assay for neutrophil chemotactic activity of lymphocyte culture supernatants.
A simplified assay was developed to determine the chemotactic activity of lymphokines for neutrophils in guinea pigs. The neutrophils migrated by chemotaxis to lymphokines through a nucleopore membrane into the lower compartment of the Boyden's chamber and were situated on the bottom cover glass. The number of the cells was counted directly under an inverted microscope. This method is simple, because the staining of the membrane can be omitted. It is also possible to follow the number of responding cells with the time lapsed in each chamber. Furthermore, the method was proved to be sensitive, quantitative and reproducible. The results obtained by this method were compared with those by the conventional methods.
D000818:Animals; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D006168:Guinea Pigs; D008214:Lymphocytes; D008222:Lymphokines / Q000032:analysis; D008722:Methods; D009504:Neutrophils
D008222:Lymphokines