The incorporation of intrastriatally injected [3H]fucose into electrophoretically separated synaptosomal glycoproteins. II. The influence of passive avoidance training.
Following intrastriatal injections of [3H]L-fucose, male albino rats served as (a) trained subjects in a step-down passive avoidance task, (b) stress controls receiving inescapable shock or (c) handled controls. At a series of time points after treatment the animals were sacrificed and the P2 fraction of the injected neostriatum was isolated. This tissue was electrophoresed on SDS-polyacrylamide gels and radioactivity profiles were constructed from 1 mm gel slices. The profiles of trained subjects were compared to shocked and handled control subjects from the same time point group. No differences in total [3H]fucose incorporation into neostriatal glycoproteins were detected as a result of the behavioral treatment used, nor was an incorporation into the majority of electrophoresed peaks altered. Three radioactive gel peaks were significantly altered as a function of experience. At the one day time point, trained  subjects exhibited a significant increase in the tritium content of a 70,000 dalton fucosyl-glycoprotein peak. At the 5 day time point, increased label was detected in a 180,000 dalton peak in both trained and shocked subjects, while a significant increase in a 140,000 dalton peak was observed only in trained animals. The relation of the present findings to perviously reported training related differences in glycoprotein metabolism are discussed.
D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D003342:Corpus Striatum / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004597:Electroshock; D005643:Fucose / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006234:Handling, Psychological; D008297:Male; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*
D006023:Glycoproteins; D005643:Fucose