Fate of mRNA following disaggregation of brain polysomes after administration of (+)-lysergic acid diethylamide in vivo.
Intravenous injection of (+)-lysergic acid diethylamide into young rabbits induced a transient brain-specific disaggregation of polysomes to monosomes. Investigation of the fate of mRNA revealed that brain poly(A+)mRNA was conserved. In particular, mRNA coding for brain-specific S100 protein was not degraded, nor was it released into free ribonucleoprotein particles. Following the (+)-lysergic acid diethylamide-induced disaggregation of polysomes, mRNA shifted from polysomes and accumulated on monosomes. Formation of a blocked monosome complex, which contained intact mRNA and 40-S plus 60-S ribosomal subunits but lacked nascent peptide chains, suggested that (+)-lysergic acid diethylamide inhibited brain protein synthesis at a specific stage of late initiation or early elongation.
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D066298:In Vitro Techniques; D008238:Lysergic Acid Diethylamide / Q000494:pharmacology*; D008297:Male; D011132:Polyribosomes / Q000187:drug effects* / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012270:Ribosomes / Q000187:drug effects; D009418:S100 Proteins / Q000096:biosynthesis
D012333:RNA, Messenger; D009418:S100 Proteins; D008238:Lysergic Acid Diethylamide