Partial purification of prorenin and activation by kallikreins: a possible new link between renin and kallikrein systems.
With the objective of identifying prorenin and its physiological activation mechanism, human plasma prorenin was completely separated from active renin for the first time. It was shown that prorenin has no activity whereas active renin has no potential for further activation. Urinary kallikreins were found to activate prorenin fractions freed from kallikrein inhibitors by affinity chromatography and treatment at pH 3.3. Human plasma kallikrein also exhibited weak activation.
D000803:Angiotensin I; D000818:Animals; D002846:Chromatography, Affinity; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D007610:Kallikreins / Q000097:blood / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology; D012083:Renin / Q000097:blood / Q000302:isolation & purification*; D013552:Swine
D004792:Enzyme Precursors; D000803:Angiotensin I; D007610:Kallikreins; D012083:Renin