The effect of diphenylhydantoin upon enzyme release in relation to collagen degradation in cat palatal mucosa during organ culture.
The effect of diphenylhydantoin (DPH) on degradation of collagen was studied during 10 days in organ culture of cat palatal mucosa by measuring the release of hydroxyproline to the culture medium. In parallel, the activities of beta-glucuronidaase and lactate dehydrogenase (LDH) as markers for lysosomal and cytosokic enzymes, respectively, were registered in the tissues and in the culture medium and the glucose consumption was also measured. DPH caused a 36% inhibition of the cumulative release of hydroxyproline to the medium. The activities in the media of beta-glucuronidase and LDH showed a 23% and 30% reduction, respectively. The glucose consumption was unaltered by the drug. The results show that one way by which DPH may interfere with collagen degradation is by blocking enzyme release from the cells.
D000818:Animals; D002415:Cats; D003094:Collagen / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D009061:Mouth Mucosa / Q000201:enzymology / Q000378:metabolism*; D009924:Organ Culture Techniques; D010160:Palate, Soft / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010672:Phenytoin / Q000494:pharmacology*
D010672:Phenytoin; D003094:Collagen; D007770:L-Lactate Dehydrogenase; D005966:Glucuronidase; D005947:Glucose; D006909:Hydroxyproline