Cellular uptake and subcellular distribution of two non-steroidal anti-inflammatory drugs: indomethacin and ketoprofen.
The cellular uptake and intracellular localization of indomethacin and ketoprofen were studied on rat embryo fibroblasts in vitro. The two drugs are taken up by the cells, but to a lesser extent for ketoprofen than for indomethacin. Inside fibroblasts ketoprofen is rapidly metabolized whereas indomethacin seems accumulated as such inside the cells. After different incubation times, ketoprofen is partly associated to the cytosol and partly to another cellular component, probably the endoplasmic reticulum. Indomethacin on the other hand is almost exclusively distributed inside the cytosol. No clear association of the two drugs can be found with the lysosomes.
D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D007213:Indomethacin / Q000378:metabolism*; D007660:Ketoprofen / Q000378:metabolism*; D010666:Phenylpropionates / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism*; D013997:Time Factors
D010666:Phenylpropionates; D007660:Ketoprofen; D007213:Indomethacin