Influence of hCG injection and steroid treatment on prostaglandin metabolism by rabbit uterus and oviduct.
Metabolism of PGE-2 and PGF-2 alpha by cytosolic fractions (100 000 g supernatant) of rabbit uterus, oviduct and lung was measured in vitro. Metabolism of PGE-2 was greater than that of PGF-2 alpha for oviduct and uterus. After an ovulating injection of hCG metabolism of both PGE-2 and PGF-2 alpha by lung and uterus declined linearly up to 72 h (during the time of ovum transport). The amount of PG metabolism by the oviduct did not change significantly during this period, but the percentage changes of PGE-2 and PGF-2 alpha metabolism from oestrous values did differ, and perhaps indicated a change in the ratio of intracellular PGs. No change of metabolism of either PG by lung, uterus or oviduct occurred at 24 or 72 h after an injection of 250 micrograms oestradiol cyclopentylpropionate given concomitantly with the hCG (a treatment regimen which causes 'tube-locking' of ova). However, progesterone treatment, in a regimen known to cause accelerated transport of ova through the oviduct, caused significantly enhanced metabolism of both PGE-2 and PGF-2 alpha by uterus and oviduct, but not lung, 30 and 48 h later except for PGE-2 by uterus at 30 h. These results suggest that changes in metabolism of PGE-2 and PGF-2 alpha by the oviduct may be involved in the mechanisms controlling ovum transport.
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D005187:Fallopian Tubes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D010066:Ovum Transport; D011374:Progesterone / Q000494:pharmacology; D011458:Prostaglandins E / Q000378:metabolism*; D011460:Prostaglandins F / Q000378:metabolism*; D011817:Rabbits; D013997:Time Factors; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D011458:Prostaglandins E; D011460:Prostaglandins F; D011374:Progesterone; D004958:Estradiol