Baculovirus replication in a mosquito (dipteran) cell line.
The baculovirus from the lepidopteran host Autographa californica (alfalfa looper) was shown to replicate in a dipteran cell line without the production of characteristic polyhedral inclusion bodies. The low level of replication could not be detected by 50% tissue culture infective dose titrations, but was apparent by [3H]thymidine labeling of the viral genome. Immunoprecipitation of the radioactive product confirmed baculovirus production.
D000330:Aedes; D000818:Animals; D002460:Cell Line; D007181:Inclusion Bodies, Viral; D007304:Insect Viruses / Q000254:growth & development*