title: Correlation between the presence of tRNA His GUG and the erythropoietic function in foetal sheep liver.
abstract: Histidyl-tRNAs from foetal and adult sheep liver were compared to their reticulocyte counterparts. The combination of various techniques revealed the existence of two histidyl-tRNA species in reticulocytes, one of which was not retained on acetylated DBAE-cellulose columns and was guanylatable. Three histidyl-tRNA isoacceptors were identified in foetal liver. Two of these species were not adsorbed on acetylated DBAE-cellulose but only one was found to be guanylatable. An identical chromatographic behaviour on RPC-5 columns was observed for guanylated histidyl-tRNAs from both origins. These results suggest the occurrence of a GUG anticodon in these guanine-accepting tRNAs. In foetal liver the amount of guanylatable histidyl-tRNA was estimated to be 7% of the total tRNA population. This observation is in agreement with the erythropoietic function of liver during the foetal life.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D000818:Animals; D004920:Erythropoiesis; D005333:Fetus; D006147:Guanine; D006639:Histidine; D008099:Liver / Q000378:metabolism*; D009928:Organ Specificity; D012343:RNA, Transfer / Q000378:metabolism*; D012156:Reticulocytes / Q000378:metabolism; D012756:Sheep; D014166:Transferases / Q000378:metabolism
chemical_list: D006639:Histidine; D006147:Guanine; D012343:RNA, Transfer; D014166:Transferases; D000604:Amino Acyl-tRNA Synthetases