Uptake and degradation of mast-cell granules by mouse peritoneal macrophages.
35S-labelled mast-cell granules isolated from mouse mastocytomas were added to mouse macrophages in vitro. The granules were avidly phagocytosed, and subsequently the radioactivity was released to the medium as inorganic [35S]sulphate. After pulse-labelling, a total of about 80% of the cell-associated radioactivity was thus released in the course of 24 h, indicating an extensive breakdown of the sulphated polysaccharides, mainly heparin, present in the granules. The uptake of the mast-cell granules caused pronounced, but reversible, spreading of the macrophages.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D003594:Cytoplasmic Granules; D006493:Heparin / Q000378:metabolism; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D008407:Mast Cells / Q000166:cytology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis
D006493:Heparin