The effect of diamide on amino acid transport by rat renal cortex slices.
Diamide directly added to renal cortical slices inhibits the uptake of amino acids. Steady-state kinetic analysis indicates an inhibition of alpha-amino acid influx without effect on efflux. The effect could be reversed by addition of pyruvate to the incubation medium. Although there was a good correlation of the transport effect of diamide with its ability to decrease cellular reduced glutathione concentration, there did not appear to be a necessary connection between them. This was shown by the fact that renal cortical slices stored at 4 degrees C have no alteration in amino acid uptake despite the fact that GSH concentration is as low as that seen with diamide. Diamide was shown to have a direct effect on the uptake of glycine by isolated renal brush border membrane vesicles.
D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D003958:Diamide / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008757:Methylglucosides / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D014633:Valine / Q000378:metabolism
D000596:Amino Acids; D000621:Aminoisobutyric Acids; D001391:Azo Compounds; D008757:Methylglucosides; D011773:Pyruvates; D003958:Diamide; D005978:Glutathione; D014633:Valine; D005947:Glucose; D005998:Glycine