Oligo(A) and double-stranded segments in polyadenylated and non-polyadenylated RNA from cytoplasm and nuclei of chick embryo.
Chick embryonic RNA was fractionated by affinity chromatography on oligo(dT)-cellulose and poly(U)-Sepharose into three classes: poly(A)+RNA containing poly(A) segments of 100 and more residues, poly(A)-oligo(A)+RNA containing oligo(A) segments of about 25 residues, and poly(A)-oligo(A)-RNA which bound to neither of the beds used and which contained double-stranded segments of 300 and more base pairs. These three classes of RNA were found in cytoplasmic as well as in heterogeneous nuclear RNA. Double-stranded segments in hnRNA, unlike those in cytoplasmic RNA, were intermolecular in nature; this may explain the occurrence of "giant" molecules in hnRNA.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D005591:Chemical Fractionation; D002642:Chick Embryo; D002846:Chromatography, Affinity; D003593:Cytoplasm / Q000032:analysis*; D011061:Poly A / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000302:isolation & purification*; D012332:RNA, Heterogeneous Nuclear / Q000302:isolation & purification
D012330:RNA, Double-Stranded; D012332:RNA, Heterogeneous Nuclear; D011061:Poly A