In vitro effects of fatty acids on the actions of serum on rat and pig cartilage.
It has been reported that fatty acids preferentially inhibit serum-stimulated incorporation of sulfate by embryonic chick cartilage, suggesting that they may interfere with the effects of a proposed mediator (serum somatomedin) of the actions of growth hormone (GH). This was studied further in mammalian cartilage. Butyrate and octanoate at concentrations of 0.5 to 5 mM produced a concentration-dependent inhibition of both basal and serum-stimulated sulfate and thymidine incorporation by costal cartilage from hypophysectomized rats. Butyrate also inhibited basal and serum-stimulated sulfate incorporation in cartilage from normal pigs and normal sucklings rats. In all 3 test systems, oleate (0.2--5 mM) bound to serum albumin (4 g/dl) was ineffective. There was no evidence that fatty acids preferentially inhibited the stimulation of sulfate incorporation produced by serum.
D000818:Animals; D001790:Blood Physiological Phenomena; D002087:Butyrates / Q000494:pharmacology; D002210:Caprylates / Q000494:pharmacology; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007016:Hypophysectomy; D008297:Male; D051381:Rats; D013045:Species Specificity; D013431:Sulfates / Q000378:metabolism*; D013552:Swine; D013936:Thymidine / Q000378:metabolism
D002087:Butyrates; D002210:Caprylates; D013431:Sulfates; D004247:DNA; D013936:Thymidine