Noncoordinate regulation of cytoplasmic RNA in compensatory renal hypertrophy.
To examine the regulatory role of mRNA in compensatory renal hypertrophy, the accumulation and decay of [3H]orotic acid in poly(A)-containing mRNA in mouse kidney was analyzed after unilateral nephrectomy during the period of maximal rRNA accretion. The distribution of radioactivity between newly synthesized poly(A)-containing and poly(A)-lacing polysomal RNA was altered, but no differences in mRNA half-life were observed in growth compared with effects of sham nephrectomy. Radioactivity in polysomal polyadenylated RNA was diminished by approximately 25% during growth where mice were labeled after nephrectomy, but if mice were labeled 18 h before operation, no difference was noted. Thus, accumulation of newly synthesized poly(A)-containing mRNA relative to RNAs that lack poly(A) is changed early in the course of renal hypertrophy. This noncoordinate regulation may represent a control mechanism effective early in induced cell growth involving mRNAs that lack poly(A).
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D006984:Hypertrophy; D007668:Kidney / Q000254:growth & development* / Q000378:metabolism; D008297:Male; D051379:Mice; D009392:Nephrectomy; D011061:Poly A; D011132:Polyribosomes / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA