[New aspects in the development and standardization of a method for determining prostaglandins in biological fluids and tissues].
A new method is described for separation of prostglandines (PG) of the A, E and F groups using microcolumns with silicic acid. Various concentrations of methanol in chloroform (2.5%, 4% and 20%) were used for reproducible elution of PG. The new method provides considerable advantages as compared with the previously published procedure. The data on quantitative estimation of PG by the newly developed method are compared with the conventional methods of column chromatography. Optimal conditions for preparation and storage of biological samples, methods for extraction of PG from blood plasma and tissues are recommended on the basis of the data published in the literature and of the results obtained.
D001826:Body Fluids / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D004355:Drug Stability; D004356:Drug Storage; D006801:Humans; D011453:Prostaglandins / Q000032:analysis* / Q000302:isolation & purification; D012016:Reference Values; D012996:Solutions; D013997:Time Factors
D011453:Prostaglandins; D012996:Solutions
Novye aspekty v razvitii i standartizatsii metoda opredeleniia prostaglandinov v biologicheskikh zhidkostiakh i tkaniakh.