Cryo-ultramicrotomy after coating of the cut surface of the tissue block with carbon and metals before sectioning.
Working under improvised conditions it is shown that coating of the cut surface of a frozen tissue block with metal and carbon improves, when applied prior to each cutting, the sectioning properties and the handling of the subsequent ultrathin cryosection.
D000818:Animals; D002244:Carbon; D007672:Kidney Cortex / Q000648:ultrastructure; D008670:Metals; D008854:Microscopy, Electron / Q000379:methods; D008867:Microtomy / Q000295:instrumentation / Q000379:methods*; D051381:Rats
D008670:Metals; D002244:Carbon