title: The occurrence of cadmium in sub-cellular particles in the kidney of the marine mussel, Mytilus edulis, exposed to cadmium. The use of electron microprobe analysis.
abstract: In mussels (Mytilus edulis) chronically exposed to cadmium, 85% of the Cd2+ was found to be associated with membrane-limited granular structures when elemental analyses were carried out on cryo-sectioned tissue by electron probe X-ray microanalysis. These granules also contained high concentrations of sulphur and phosphorus as well as other metalions, including Ca2+, iron and Zn2+. In contrast, after homogenisation and fractionation by differential centrifugation, the major proportion of the Cd2+ was found in the cytoplasmic fraction. However, many lysosomes were also ruptured by this treatment. Gel filtration chromatography of this fraction indicated the presence of a Cd2+-binding component of similar molecular weight to the metallothionein purified from the digestive gland of the same animals. It is therefore proposed that metallothionein may be associated with particulate structures which would thus reduce its cellular toxicity.
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D007668:Kidney / Q000378:metabolism* / Q000648:ultrastructure; D013052:Spectrometry, X-Ray Emission; D013347:Subcellular Fractions / Q000378:metabolism*
chemical_list: D002104:Cadmium