[Use of the combined Kjeldahl-wet digestion/Berthelot-reaction for determining nitrogen in biological materials. 2. Design and testing of a partially automated apparatus for routine determination of nitrogen].
The authors describe the design and the mode of operation of a partially automated analyzer system, based on the combination of the Kjeldahl wet digestion with the Berthelot reaction, for the determination of nitrogen in biological materials. The results from three years of operation are discussed from the view-points of variability of the materials to be tested, of accuracy and of precision. This analytical technique (with an accuracy of 98% and a precision of 4%) is suited for determining amino and amido nitrogen as well as non-aromatically bound ring nitrogen. The statistical evaluation of the analytical results revealed that the deviation of the measured values is mainly caused by the high-temperature digestion.
D000577:Amides / Q000032:analysis; D000588:Amines / Q000032:analysis; D001322:Autoanalysis; D005504:Food Analysis / Q000379:methods; D005971:Glutamates / Q000032:analysis; D006358:Hot Temperature; D009584:Nitrogen / Q000032:analysis*; D011506:Proteins / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000032:analysis
D000577:Amides; D000588:Amines; D005971:Glutamates; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D009584:Nitrogen
Zur Anwendung der Methodenkombination Kjeldahl-Nassaufschluss/Berthelot-Reaktion bei der Stickstoffbestimmung in biologischen Materialien. 2. Mitt. Aufbau und Erprobung einer teilautomatisierten Apparatur zur routinemässigen Bestimmung des Stickstoffs.