Isolation of influenza C virus recombinants.
Recombinants between two different influenza C viruses were isolated. In MDCK (canine kidney) cells, one strain, C/JJ/50, caused lytic plaques, whereas C/JHG/66 virus did not produce clear plaques. From a mixed infection of MDCK cells with C/JHG/66 virus and UV-inactivated C/JJ/50 virus, clones were isolated which possessed the clear-plaque phenotype. Fingerprint analyses indicated that the RNAs of parent viruses had different oligonucleotide patterns and that one of the clones derived from the mixed infection was formed by reassortment of parental genes. This recombinant clone most likely inherited RNAs 1, 2, 3, 6, and 7 from C/JGH/66 virus and RNAs 4 and 5 from C/JJ/50 virus.
D000818:Animals; D002460:Cell Line; D004285:Dogs; D005814:Genes, Viral; D007668:Kidney; D009843:Oligoribonucleotides / Q000032:analysis; D009975:Orthomyxoviridae / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D011995:Recombination, Genetic; D010948:Viral Plaque Assay
D009843:Oligoribonucleotides; D012367:RNA, Viral