The energy metabolism of the leucocyte. X. Kinetics of oxygen consumption during phagocytosis by polymorphonuclear leucocytes. A photometric method.
A simple micromethod for quantitative assay of the oxygen consumption by phagocytosing human neutrophils had been described. The continuous assay shows a lag time between the addition of zymosan and the maximal rate of oxygen uptake, which is a measure of the serum opsonic activity. Several factors can alter the oxygen uptake and also the delay of response.
D000654:Amobarbital / Q000494:pharmacology; D003080:Cold Temperature; D003847:Deoxyglucose / Q000494:pharmacology; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D009895:Opsonin Proteins; D010101:Oxygen Consumption; D010108:Oxyhemoglobins / Q000378:metabolism; D010587:Phagocytosis; D012969:Sodium Fluoride / Q000494:pharmacology; D013053:Spectrophotometry; D013997:Time Factors; D015054:Zymosan / Q000378:metabolism
D009895:Opsonin Proteins; D010108:Oxyhemoglobins; D012969:Sodium Fluoride; D015054:Zymosan; D003847:Deoxyglucose; D000654:Amobarbital