Separation of individual sulfated bile acid conjugates as calcium complexes using reversed-phase partition thin-layer chromatography.
A method for separating individual monosulfated primary bile acid conjugates by reversed-phase partition thin-layer chromatography on octadecyl-bonded silica gel is described. The solvent system is acetonitrile containing calcium, probably as calcium carbamate. Excellent resolution of the 3- and 7-monosulfated glycine conjugates, as well as 3- and 7-monosulfated taurine conjugates of cholic and chenodeoxycholic acids is reported. A convenient class separation of sulfated from nonsulfated primary bile acid conjugates by adsorption thin-layer chromatography on low-polarity silica gel is also described.
D001647:Bile Acids and Salts / Q000302:isolation & purification*; D002118:Calcium; D002855:Chromatography, Thin Layer; D006801:Humans; D008722:Methods
D001647:Bile Acids and Salts; D002118:Calcium