Novobiocin; an inhibitor of the repair of UV-induced but not X-ray-induced damage in mammalian cells.
In addition to inhibiting replicative DNA synthesis in HeLa cells, novobiocin has a severe effect on the cellular response to UV irradiation, reducing the number of breaks made in pre-existing DNA by the excision repair process. The inhibition of UV repair by novobiocin is reflected in enhanced UV-killing of these cells. Rejoining of DNA after X irradiation is not impaired by novobiocin. The recognition and removal of UV damage may require unwinding of the DNA by gyrase, which--in bacteria--is the target for novobiocin.
D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects*; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D007700:Kinetics; D009675:Novobiocin / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014965:X-Rays
D009675:Novobiocin