Technical improvements in the clotted plasma droplet MIF assay in vitro.
The migration inhibitory activity of culture supernatants of rat and human lymphocytes stimulated with PHA and Con A-Sepharose was tested on cell migration from clotted plasma droplets. This technique was improved by using homologous as well as heterologous plasma and fibrinogen solutions for suspending the migratory cells, automatic micropipettes for performing the technique and purified cell populations as target. The effects of calcium chloride and of the cell concentration in the plasma droplets on the migration indices obtained in the MIF assay were tested.
D000818:Animals; D001777:Blood Coagulation; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D005340:Fibrinogen; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D010949:Plasma; D051381:Rats
D010835:Phytohemagglutinins; D003208:Concanavalin A; D005340:Fibrinogen