Tumorigenicity and intracisternal A-particle expression of hybrids between murine myeloma and lymphocytes.
Hybrids of BALB/c lymphocytes and a murine myeloma, a tumor that expresses intracisternal A-particles, were obtained with polyethylene glycol as the fusogen. The karyotype, tumorigenicity, and A-particle expression of the hybrid clones were assessed. All the hybrid clones analyzed were tumorigenic and expressed intracisternal A-particles even when they were the result of a fusion event between two lymphocytes and one myeloma cell in which no loss of chromosomes was detected. The tumors that developed following inoculation of hybrid cells into BALB/c mice (1 x 10(6) cells/mouse) were karyotypically identical to the inoculated cells. It appears that the two myeloma cell phenotypic traits analyzed (tumorigenicity and A-particle expression) are dominant.
D000818:Animals; D002875:Chromosomes; D005799:Genes, Dominant; D006822:Hybrid Cells / Q000382:microbiology*; D007181:Inclusion Bodies, Viral; D008214:Lymphocytes / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000235:genetics / Q000382:microbiology*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000382:microbiology; D014184:Transplantation, Homologous