[Effects of specific reagents and urea on the reactivity of non-heme iron and thiol groups of pea and corn ferredoxins].
The reactivities of the SH-groups of pea and corn ferredoxins were found to be different. One or two SH-groups in the molecule of pea ferredoxin and one SH-group in the molecule of corn ferredoxin are readily available for the thyol group specific reagents. Four SH-groups of both ferredoxins are completely masked, i. e. available for the thyol reagents only after protein denaturation in the presence of urea. The rates of SH-group interaction with the sulfhydryl reagents in corn ferredoxin are lower than those in pea ferredoxin. The non-haem iron of pea ferredoxin interacts with the complex formers far more rapidly as compared to corn ferredoxin. The ferredoxins tested differ in the amount of iron atoms. The latter require the presence of oxygen for their complete interaction with the complex formers.
D005288:Ferredoxins / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D010944:Plants / Q000378:metabolism*; D011485:Protein Binding; D013045:Species Specificity; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D014508:Urea / Q000494:pharmacology*
D005288:Ferredoxins; D013439:Sulfhydryl Reagents; D014508:Urea; D007501:Iron
Reaktsionnaia sposobnost' negemovogo zheleza i tiolovykh grupp ferredoksinov gorokha i kukuruzy pri deĭstvii spetsificheskikh reagentov i mocheviny.