[Determination of the fibrinolytic activity of renal tissue in children].
A method for the determination of the fibrinolytic activity of renal tissue obtained by puncture biopsy is suggested. Three cryostate sections are placed on slides, covered by a film of a mixture of bovine fibrinogen and thrombine in saline (in the control section plasminogen activator is pre-destroyed), placed in a thermostate at 37 degrees C for 24 hours, stained with Ehrlich hematoxylin and embedded in glycerol-gelatine. The fibrinolytic activity is evaluated by calculating the ratio expressing the ratio between the lysis surface and the surface of the kidney cortex section. The advantage of the method lies in the technical simplicity combined with the objective evaluation of the results.
D002648:Child; D005342:Fibrinolysis; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000502:physiology*; D008722:Methods
Opredelenie fibrinoliticheskoĭ aktivnosti pochechnoĭ tkani u deteĭ.