Limulus amebocyte lysate - a means to monitor inactivation of lipopolysaccharide.
A properly selected Limulus Amebocyte Lysate (LAL) has been demonstrated to have a good correlation with the in vivo United States Pharmacopeial pyrogen test when testing lipopolysaccharide (LPS) inactivated with ethylene oxide, 60 Co irradiation, and dry-heat treatments. Thus, LAL may be used to determine degradation or depyrogenation kinetics of LPS. A criteria for acceptance of an LAL lot for correlation with the USP pyrogen test is described.
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis; D001681:Biological Assay; D003037:Cobalt Radioisotopes; D004731:Endotoxins / Q000032:analysis; D005027:Ethylene Oxide; D005720:Gamma Rays; D006358:Hot Temperature; D007700:Kinetics; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D011749:Pyrogens / Q000032:analysis; D011817:Rabbits; D013329:Structure-Activity Relationship
D001427:Bacterial Toxins; D003037:Cobalt Radioisotopes; D004731:Endotoxins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D011749:Pyrogens; D005027:Ethylene Oxide