Cleavage of elongation factor G into compact domains.
A limited trypsinolysis of native elongation factor G results in the formation of two large fragments resistant to further proteolysis. The fragments were isolated in homogeneous state in conditions when their native structure is retained. According to circular dichroism and scanning calorimetry data the formed fragments retain the stability and compact structure that they had in the whole protein.
D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D046911:Macromolecular Substances; D008970:Molecular Weight; D010445:Peptide Elongation Factors / Q000032:analysis*; D010446:Peptide Fragments; D014357:Trypsin
D046911:Macromolecular Substances; D010445:Peptide Elongation Factors; D010446:Peptide Fragments; D014357:Trypsin