Inhibition of influenza virus uncoating by rimantadine hydrochloride.
In freeze-thaw lysates of MDCK cells infected with 32P-labeled influenza virus A/WSN in the presence of added RNase, acid-precipitable radioactivity diminished to about 50% of initial values within 90 min after a 1-h virus adsorption period. A similar preparation containing rimantadine at a concentration of 50 micrograms/ml exhibited only a 10% reduction in acid-precipitable radioactivity. These findings suggest that rimantadine interferes with uncoating of influenza virus in infected cells.
D000218:Adamantane / Q000031:analogs & derivatives*; D000327:Adsorption; D000818:Animals; D002460:Cell Line; D004285:Dogs; D009980:Influenza A virus / Q000187:drug effects* / Q000254:growth & development; D007668:Kidney; D012260:Ribonucleases / Q000378:metabolism; D012299:Rimantadine / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D012299:Rimantadine; D012260:Ribonucleases; D000218:Adamantane