title: The responsiveness of efferent lymph cells to phytohaemagglutinin during the response of the popliteal node to dinitrophenylated bovine serum albumin.
abstract: Cells, recovered from the efferent lymphatics of the popliteal nodes of sheep during in vivo responses to dinitrophenylated bovine serum albumin (DNP-BSA), were examined for their responsiveness to phytohaemagglutinin (PHA) in vitro during the first 4--5 days of the immune response. The response was almost totally depressed when cells collected throughout the response were cultured with supra-optimal doses of the mitogen. Optimal and suboptimal doses of PHA resulted in greatly enhanced responses in cells collected in the first two periods (0--6 h; 6--12h) of the response; the presence of high doses of DNP-BSA in the culture media prevented the enhancement in the second period but had no adverse effects on the cells collected during the first 6 h. Efferent cells collected after 12 h generally showed decreased responses, this depression being maximal in cells collected 2--3 days after antigenic stimulation. Later in the response the cells again exhibited enhanced responses. The possible interpretations of these results in terms of regulation of the response are discussed.
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D004136:Dinitrobenzenes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D008196:Lymph / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D009578:Nitrobenzenes / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000276:immunology*; D012756:Sheep; D013997:Time Factors
chemical_list: D000941:Antigens; D004136:Dinitrobenzenes; D009578:Nitrobenzenes; D010835:Phytohemagglutinins; D012710:Serum Albumin, Bovine