Chromosome analysis of primary large bowel tumors: a new method for improving the yield of analyzable metaphases.
Colon tumors were processed immediately after surgical resection. Using L-Arterenol and sodium citrate in combination with standard chromosome culture techniques, 12 of 17 consecutive tumors (75%) had countable figures ranging from 5 cells to 59 cells. Seven of the 12 cases had over 25 countable cells. Chromosome counts did not vary more than +/- 4 for any one case. Seven tumors were karyotyped, 6 with banding techniques. One of these specimens had mosaic patterns. The chromosome counts varied: 2 were hypodiploid; 1 was pseudodiploid and 4 were hyperdyploid. There were some similarities in the karotypes although no identical karyotypes were seen in this series.
D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods*; D002951:Citrates; D003110:Colonic Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D009638:Norepinephrine
D002951:Citrates; D009638:Norepinephrine