Scintillation fluid shortens exposure times in autoradiography.
Premixed, commercially available scintillation fluids were used to reduce exposure time of tritium (3H) and iodine-125 (125I)-labeled whole cells, and of 3H-labeled Giemsa-banded chromosome preparations. Emulsion-coated slides were dipped into scintillator for no longer than 2 min and exposed in the dark at 4 degrees C. Maximal values for percentage of human diploid cell (WI-38) nuclei labeled with 3H-thymidine of moderate specific activity were obtained in 12 hr. Without scintillator the exposure time was 4 days. Exposure time for cells labeled with 125I-serum was reduced from over 90 days to 14 days. The shortened exposure time for banded chromosomes permitted successful prestaining with Giemsa, a sequence that is not possible without scintillator.
D001345:Autoradiography; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure*; D006651:Histocytochemistry; D006801:Humans; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D007621:Karyotyping; D008168:Lung / Q000196:embryology; D012588:Scintillation Counting; D014316:Tritium
D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D014316:Tritium