title: Cell-cycle inhibition by misonidazole of human cells cultivated in vitro under aerobic conditions.
abstract: By means of flow cytometric recording of DNA histograms and counting of cells in synchronized populations, we have found that misonidazole (MIS) in clinically relevant concentrations induces cell-kinetic changes in human cells (NHIK 3025) cultivated in vitro under aerobic conditions. The effect seems to be a general lengthening of the cell cycle, affecting all phases. However, induction of this effect is phase-dependent, since only cells exposed to MIS during mitosis and/or early G1 will suffer significant cell-cycle prolongation. In exponentially growing populations this effect of MIS leads to a transient increase in the fraction of G1 cells and a corresponding decrease in the fraction of S cells. The possible significance of this effect for the clinical use of MIS is discussed.
mesh_terms: D000332:Aerobiosis; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000032:analysis; D006801:Humans; D007399:Interphase / Q000187:drug effects; D008920:Misonidazole / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D009593:Nitroimidazoles / Q000494:pharmacology*
chemical_list: D009593:Nitroimidazoles; D008920:Misonidazole; D004247:DNA