A modified non-radioisotope method for measurement of platelet production time.
Platelet production time (PPT), based on the measurement of malondialdehyde (MDA) production prior to and after the intake of acetylsalicylic acid (ASA), was determined in 20 healthy subjects. High MDA levels were produced by stimulating platelet lipid peroxidation with arachidonic acid, resulting in a reliable, sensitive and accurate technique. PPT correlated well with platelet survival time measured by 51Cr autologous labelled platelets when both methods were used simultaneously in the same patients. This modified non-radioisotope method might serve as a useful aid in the diagnosis of platelet disorders, thromboembolic diseases associated with increased platelet consumption and for the evaluation of drugs affecting platelet function.
D001241:Aspirin / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism; D002470:Cell Survival; D006410:Hematopoiesis; D006801:Humans; D050356:Lipid Metabolism; D008315:Malondialdehyde / Q000097:blood; D010979:Platelet Function Tests / Q000379:methods*; D013997:Time Factors
D008315:Malondialdehyde; D001241:Aspirin