title: Structural transitions of chromatin induced by netropsin.
abstract: Structural changes of chromatin induced by interaction of netropsin (Nt) with DNA has been examined by analysis of CD and electromicroscopic measurements. The results demonstrate the existence of a transition from the condensed globular state of chromatin into nucleosomal fibres generated by extremely low Nt concentration up to 1 mole Nt per 200 nucleotides. A second transition occurs at high Nt ratio per DNA phosphate (v' = 0.3). involving disorganization of nucleosomal particles. The interference of the Nt binding with chromatin proteins maintaining the sub- and superstructure will be discussed.
mesh_terms: D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002843:Chromatin / Q000648:ultrastructure*; D002942:Circular Dichroism; D004247:DNA; D006146:Guanidines / Q000494:pharmacology; D007700:Kinetics; D008854:Microscopy, Electron; D009429:Netropsin / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D013950:Thymus Gland
chemical_list: D002843:Chromatin; D006146:Guanidines; D009429:Netropsin; D004247:DNA