[Biosynthesis of the cell wall polysaccharides, mannan and glucan, by Candida spec. H as indication of different pathways of glucose breakdown].
The catabolic and anabolic D-glucose transformation of the yeast Candida spec. H has been studied. By using 1-14C-D-glucose and 6-14C-D-glucose, measuring the 14CO2 liberation and the label of glucose and mannose isolated from glucan and mannan, the following results have been obtained.1. Beginning with 100 micromoles glucose . ml-1 in the batch growth medium, at first on an average 64% of the glucose having been catabolized to CO2 are directly decarboxylated to pentose phosphate by pentose phosphate pathway (PPW). Later on at an exogen concentration of 70 micromoles.ml-1 73% of glucose on an average having been catabolised to CO2 undergoes transformation via glycolyse and tricarbonacid cycle (G-TCC). 2. Only after getting this glucose concentration the maximal hexose incorporation rate into glucan and mannan can be obtained. 3. 20--40% of the hexose channeled into the polysaccharid-biosynthesis have been prepared by resynthesis from pentose phosphate via PPW. 4. The results are discussed in connection with the observed crabtree effect.
D002175:Candida / Q000378:metabolism*; D002245:Carbon Dioxide / Q000096:biosynthesis; D002473:Cell Wall / Q000378:metabolism; D002952:Citric Acid Cycle; D005285:Fermentation; D005936:Glucans / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D008351:Mannans / Q000096:biosynthesis*; D010428:Pentosephosphates / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis*
D005936:Glucans; D008351:Mannans; D010428:Pentosephosphates; D011134:Polysaccharides; D002245:Carbon Dioxide; D005947:Glucose
Biosynthese der Zellwand-Polysaccharide Mannan und Glucan als Spiegelbild unterschiedlicher Abbauwege der Glucose durch Candida spec. H.