[Peculiarities of histones H1, H2b and H4 structure and aggregation in the presence of glycine].
The efficiency of intramolecular and intermolecular interactions of histons H1, H2b and H4 was studied as affected by the glycine concentration in the composition of the glycine-HCl buffer (pH 3.0). It is shown that the concentration of glycine less than 0.01m should be used in experiments with the glycine buffer. Glycine in a higher concentration evokes a considerable aggregation of the histon molecules. Sizes and shape of the aggregates differ in solutions with the lower and high ionic strength. In the presence of glycine the sizes of histone aggregates decrease in the series H1 greater than H2b greater than H4. Changes in the quantum yield of histon fluorescence reflect changes in the tertiary structure of their molecule but not in aggregation.
D000818:Animals; D002417:Cattle; D005998:Glycine; D006657:Histones; D007700:Kinetics; D046911:Macromolecular Substances; D009994:Osmolar Concentration; D011485:Protein Binding; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence
D006657:Histones; D046911:Macromolecular Substances; D005998:Glycine
Osobennosti struktury i agregatsii gistonov H1, H2b i H4 v prisutstvii glitsina.