title: A semi-automated Bratton & Marshall micromethod for determining acetylator phenotype of rabbits using the Abbott Bichromatic Analyzer-100.
abstract: A micromethod for the determination of acetylator phenotype using the method of Bratton & Marshall (1939) J. Biol. Chem. 128, 537--550) was adapted to the Abbott Bichromatic Analyzer (ABA-100). Advantages of this procedure include: (a) the requirement for small blood samples (20 microliter) making it a suitable pediatric method and eliminating the need for venipunctures; (b) elimination of tedious manual reading of absorbances of samples and mathematical manipulation of data by the automated capabilities of the ABA-100; (c) an increase in the accuracy of the assay by reading samples at two wavelengths thereby correcting for differences in background.
mesh_terms: D000107:Acetylation; D000818:Animals; D001322:Autoanalysis; D006207:Half-Life; D008722:Methods; D008832:Microchemistry; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010641:Phenotype; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D013411:Sulfadiazine / Q000097:blood
chemical_list: D004364:Pharmaceutical Preparations; D013411:Sulfadiazine