[Hepatitis B vaccine: characterization of the polypeptides of hepatitis B surface antigen (author's transl)].
In order to realize a new kind of hepatitis B vaccine, the polypeptide components of the outer-coat of the hepatitis B virus have been studied. The characterization of the polypeptides of purified hepatitis B surface antigen (HBsAg) was performed by SDS-polyacrylamide gel electrophoresis. Seven polypeptides were observed, designated P1 to P7, according to their increasing molecular weight: P1 (MW = 24,000), P2 (MW = 32,500) and P6 (MW = 78,000) represented the major components. When the reducing action was increased and followed by alkylation and citraconylation, a single polypeptide of low MW (5,000 less than MW less than 10,000) was isolated from HBsAg.
D004591:Electrophoresis, Polyacrylamide Gel; D006509:Hepatitis B / Q000517:prevention & control*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D010455:Peptides / Q000032:analysis*; D014765:Viral Vaccines
D006514:Hepatitis B Surface Antigens; D010455:Peptides; D014765:Viral Vaccines
Vaccin contre l'hépatite B: caractérisation des polypeptides constitutifs de l'enveloppe du virus de l'hépatite B.