[Measuring the activity of the placental isoenzyme of alkaline phosphatase: 3 methods compared (author's transl)].
Three methods were compared in human serum for determining the activity of the placental isoenzyme of alkaline phosphatase: 1. Heat inactivation at 65 degrees C for 10 minutes, 2. Differential inactivation with L-p-Bromotetramisole, and 3. an immunological precipation test. A good comparison between the three methods was found (correlation coefficient between 0.973 and 0.982). For long series or screening determinations for "Regan-isoenzyme" the differential inactivation with L-p-Bromotetramisole is preferred because of the short analysis time and the possibility of simple mechanisation.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D007118:Immunoassay; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008722:Methods; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013773:Tetramisole / Q000031:analogs & derivatives / Q000494:pharmacology
D007527:Isoenzymes; C006226:4-bromotetramisole; D013773:Tetramisole; D000469:Alkaline Phosphatase
Aktivitätsbestimmung des placentalen Isoenzyms der alkalischen Phosphatase im Serum: Ein Methodenvergleich.