[Rhythm of protein concentration and incorporation of 3H-lysine into hepatocytes in vitro].
"In vitro" incubated slices of the rat liver, by the method of interferometry combined with radioautography, have demonstrated hour's rhythmic fluctuations in protein concentration and 3H-lysine incorporation into proteins. The biochemical method has demonstrated fluctuations in protein synthesis rate in the same cells with an average period of about one hour. The possibility to apply the interferometric method in combination with radioautography for revealing quantitative changes of protein in slices of organic cultures is demonstrated.
D000818:Animals; D001345:Autoradiography; D008099:Liver / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008297:Male; D008857:Microscopy, Interference; D009924:Organ Culture Techniques; D010507:Periodicity; D011506:Proteins / Q000378:metabolism*; D051381:Rats
D011506:Proteins; D008239:Lysine
Ritm kontsentratsii belkov i vkliucheniia 3H-lizina v gepatotsity in vitro.