title: Use of the overturn end point in goldfish to measure the interaction of diazepam and ethanol and the effects of the binding of diazepam to bovine serum albumin.
abstract: Over the ranges 2.8 X 10(-5) to 8.78 X 10(-5) M diazepam and 4.85 X 10(-2) to 1.22 X 10(-1) M ethanol, addition of the effects of these agents on the overturn end point in goldfish was observed. The addition of bovine serum albumin (1.56 X 10(-5) M) to aqueous solutions of diazepam modifies the diazepam effect by reducing the "free" drug concentrations.
mesh_terms: D000818:Animals; D002417:Cattle; D003530:Cyprinidae / Q000502:physiology*; D003975:Diazepam / Q000097:blood / Q000494:pharmacology*; D004347:Drug Interactions; D000431:Ethanol / Q000494:pharmacology*; D006054:Goldfish / Q000502:physiology*; D004856:Postural Balance / Q000187:drug effects*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D012710:Serum Albumin, Bovine; D000431:Ethanol; D003975:Diazepam