Inhibition of D-glyceraldehyde-3-phosphate dehydrogenase by ATP and quinaldate.
The inhibition of D-glyceraldehyde-3-phosphate dehydrogenase by ATP is of purely mixed type with respect to NAD (Ki=4.9 mM), purely uncompetitive with respect to D-glyceraldehyde-3-phosphate (Ki=9.4 mM) and partially uncompetitive with respect to inorganic phosphate (Ki=6.0 mM). Quinaldate is a purely mixed type inhibitor with respect to both NAD (Ki==10.0 mM) and D-glyceraldehyde-3-phosphate (Ki=15.3 mM), whereas purely non-competitive with respect to inorganic phosphate (Ki=11.0 mM). In the presence of quinaldate a lag period is observed in the time course of enzyme reaction. The duration of this lag period depends on both quinaldate and substrate concentrations.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002264:Carboxylic Acids / Q000494:pharmacology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D009132:Muscles / Q000201:enzymology; D011804:Quinolines / Q000494:pharmacology*; D013552:Swine
D002264:Carboxylic Acids; D011804:Quinolines; D000255:Adenosine Triphosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases