The primary isolation of Leishmania donovani from Iraq on different culture media.
Leishmania donovani was isolated by culturing bone marrow aspirates from kala-azar patients, on three types of culture media. The isolation was successful in 88% of the cases. The effects of pH, D-glucose, L-proline and antibiotic concentration in the isolation were investigated. The semi-solid medium used (pH 7.4) with L-proline, was better than the modified NNN media for the primary isolation.
D001853:Bone Marrow / Q000469:parasitology; D002675:Child, Preschool; D003470:Culture Media; D006801:Humans; D007223:Infant; D007493:Iraq; D007891:Leishmania / Q000254:growth & development / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000469:parasitology*
D003470:Culture Media