Cross-linked informofers.
The proteins of 30S RNP particles containing pre-mRNA (hnRNA) were cross-linked with bifunctional reagents (dimethyl-suberimidate and dimethyl-3,3'-dithiobispropionimidate). Further treatment with 1 or 2 M NaCl dissociates all RNA from protein. However, a significant part of protein particles--informofers being cross-linked survived high salt treatment. Their sedimentation coefficients were close to those of original particles. No RNA could be detected in the informofers even after labeling the cells with a precursor for a long period of time. Sodium dodecylsulfate or urea dissociated cross-linked informofers into oligomeric polypeptides. They could be dissociated by beta-mercaptoethanol treatment if a reversible cross-linked reagent had been used. The resulting polypeptides were represented by informatin. RNP particles (30S RNP or poly-particles) were reconstituted upon mixing of cross-linked informofers with pre-mRNA and removal of 2 M NaCl.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D003432:Cross-Linking Reagents; D004120:Dimethyl Suberimidate; D004220:Disulfides; D004591:Electrophoresis, Polyacrylamide Gel; D007096:Imidoesters; D008099:Liver / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009698:Nucleoproteins / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D012261:Ribonucleoproteins / Q000378:metabolism
D003432:Cross-Linking Reagents; D004220:Disulfides; D007096:Imidoesters; D009698:Nucleoproteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D004120:Dimethyl Suberimidate; C007471:dimethyl dithiobispropionimidate