Comparison of avidin induction in the differentiated and undifferentiated chick oviduct by progesterone, actinomycin D and oviductal injury.
Various oestrogen (diethylstilboestrol, DES) pre-treatments were carried out on chicks and the production of avidin in the oviduct was induced by progesterone, actinomycin D or oviductal injury. Avidin induction was dose-dependent at doses between 5 mg and 40 mg progesterone/kg or 50 micrograns and 300 micrograms actinomycin D/kg respectively. The induction by oviductal injury correlated with the magnitude of tissue injury. First signs of avidin induction were seen at 4 h after oviductal injury, 12 h after progesterone or 12--16h after actinomycin D administration. Actinomycin D (200 micrograms/kg), when administered after progesterone injection, did not increase avidin induction by progesterone, this indicating that avidin induction by actinomycin D is not a "superinduction" effect. Evidence is presented here that the mechanism of avidin induction by oviductal injury and actinomycin D differs from that by progesterone. The differentiation of the oviduct caused by DES treatment was necessary for the induction by progesterone, whereas actinomycin D and oviductal injury also induced avidin in the undifferentiated or poorly differentiated oviduct. Simultaneous DES stimulation potentiated induction by progesterone but not by actinomycin D or oviductal injury. Furthermore, single prior DES stimulation increased avidin induction in the differentiated oviduct of DES-withdrawn chicks caused by progesterone but not that by actinomycin D or oviductal injury.
D000818:Animals; D001360:Avidin / Q000096:biosynthesis*; D002645:Chickens; D003609:Dactinomycin / Q000494:pharmacology*; D004054:Diethylstilbestrol / Q000494:pharmacology; D010047:Ovalbumin / Q000031:analogs & derivatives*; D010057:Oviducts / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*
D001360:Avidin; D003609:Dactinomycin; D011374:Progesterone; D004054:Diethylstilbestrol; D010047:Ovalbumin