[Kinetic parameters of superhelical DNA cleavage by endonuclease S1].
Major kinetic parameters of endonuclease S1 were determined on superhelical bacteriophage PM2 DNA and on relaxed nicked circular PM2 DNA. At 37 degrees and 0,25 M NaCl, the Michaelis constants were respectively 1.7 . 10(-8) M and 1 . 10(-9) M, and catalytic constants were respectively 0.36 sec-1 and 1.2 . 10(-2) sec-1. The inhibition of the enzyme reaction by its product was detected.
D001236:Aspergillus oryzae / Q000201:enzymology; D001435:Bacteriophages; D004270:DNA, Circular; D004278:DNA, Superhelical; D004279:DNA, Viral; D003851:Deoxyribonucleases / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*; D007700:Kinetics; D011549:Pseudomonas
D004270:DNA, Circular; D004278:DNA, Superhelical; D004279:DNA, Viral; D003851:Deoxyribonucleases; D004720:Endonucleases
Kineticheskie parametry rasshchepleniia superspiral'noĭ DNK éndonukleazoĭ S1.