Isolation and characterization of a rubredoxin and two ferredoxins from Desulfovibrio africanus.
Rubredoxin and two distinct ferredoxins have been purified from Desulfovibrio africanus. The rubredoxin has a molecular weight of 6000 while the ferredoxins appear to be dimers of identical subunits of approximately 6000 to 7000 molecular weight. Rubredoxin contains one iron atom, no acid-labile sulfide and four cysteine residues per molecule. Its absorbance ratio A278/A490 is 2.23 and its amino acid composition is characterized by the absence of leucine and a preponderance of acidic amino acids. The two ferredoxins, designated I and II, are readily separated on DEAE-cellulose. The amino acid compositions of ferredoxins I and II show them to be different protein species; the greater number of acidic amino acid residues in ferredoxin I than in ferredoxin II appears to account for separation based on electronic charge. Both ferredoxins contain four iron atoms, four acid-labile residues per molecule. Spectra of the two ferredoxins differ from those of ferredoxins of other Desulfovibrio species by exhibiting a pronounced absorption peak at 283 nm consistent with an unusual high content of aromatic residues. The A385/A283 absorbance ratio of ferredoxins I and II are 0.56 and 0.62, respectively. The N-terminal sequencing data of the two ferredoxins clearly indicate that ferredoxins I and II are different protein species. However, the two proteins exhibit a high degree of homology.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D003901:Desulfovibrio / Q000032:analysis*; D005288:Ferredoxins / Q000302:isolation & purification; D007501:Iron / Q000032:analysis; D008970:Molecular Weight; D012416:Rubredoxins / Q000302:isolation & purification; D013053:Spectrophotometry
D000596:Amino Acids; D005288:Ferredoxins; D012416:Rubredoxins; D007501:Iron