Growth and differentiation of aggregating fetal brain cells in a serum-free defined medium.
Aggregating cultures of mechanically dissociated fetal brain cells provide an excellent system for neurobiological studies of cellular growth and differentiation, but, in common with almost all culture systems, they have the disadvantage that crude serum is required in the medium. Although several cell lines have either been adapted to serum-free conditions or grown normally in serum-free media supplemented with hormones, trace elements and defined serum components, this approach has never been applied to differentiating primary cells of the central nervous system. We now describe the successful cultivation of aggregating fetal rat brain cells in a chemically defined, serum-free medium.
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D002449:Cell Aggregation; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D005971:Glutamates / Q000378:metabolism; D006133:Growth Substances; D051381:Rats
D003470:Culture Media; D005971:Glutamates; D006133:Growth Substances