title: Cryo-ultramicrotomy after coating of the cut surface of the tissue block with carbon and metals before sectioning.
abstract: Working under improvised conditions it is shown that coating of the cut surface of a frozen tissue block with metal and carbon improves, when applied prior to each cutting, the sectioning properties and the handling of the subsequent ultrathin cryosection.
mesh_terms: D000818:Animals; D002244:Carbon; D007672:Kidney Cortex / Q000648:ultrastructure; D008670:Metals; D008854:Microscopy, Electron / Q000379:methods; D008867:Microtomy / Q000295:instrumentation / Q000379:methods*; D051381:Rats
chemical_list: D008670:Metals; D002244:Carbon