title: Reverse phase high pressure liquid chromatographic determination of some iodoamino acid contaminants in sodium liothyronine or sodium levothyroxine.
abstract: Some iodoamino acids have been separated and determined by high pressure liquid chromatography on octadecylsilane reverse phase packing with a mobile phase consisting of methanol, potassium phosphate monobasic, and orthophosphoric acid at 44 degrees C. The method separates and quantitates mixtures of 3,5-diiodothyronine, liothyronine, isoliothyronine, and levothyroxine. The procedure provides an accurate, sensitive, and rapid estimation of decomposition and/or impurities in standards in approximately 30 min, at the less than 75 pmole level, using an ultraviolet detector at 254 nm.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004104:Diiodothyronines / Q000032:analysis; D004340:Drug Contamination; D013974:Thyroxine / Q000032:analysis*; D014284:Triiodothyronine / Q000032:analysis*
chemical_list: D000596:Amino Acids; D004104:Diiodothyronines; D014284:Triiodothyronine; D013974:Thyroxine