Ozone sensitivity in aging WI-38 cells based on acid phosphatase content.
Young [16-19 population doubling level (PDL)] and senescing (50-53 PDL) WI-38 cell populations were exposed to 1 ppm ozone for 2 hr and the resultant extracellular and intracellular acid phosphatase concentration was measured. Dose-response curves were also determined for surviving populations of young and old cells after a 1 hr ozone exposure ranging in concentration from 0 to 1.00 ppm. Senescing cells released 8 times more acid phosphatase per million cells than the young cells. Both old and young cells showed a clear dose-response to the 1 hr ozone gradient exposure. However, the older cells demonstrated a consistent 17% average lower survival rate than the young cells. The higher acid hydrolase level in older WI-38 cells is probably related to the lower survival rate observed in the older cells in vitro.
D000135:Acid Phosphatase / Q000378:metabolism*; D000367:Age Factors; D002460:Cell Line; D006801:Humans; D008168:Lung / Q000201:enzymology*; D010126:Ozone / Q000633:toxicity*
D010126:Ozone; D000135:Acid Phosphatase