Use of chromosomal translocations with in situ DNA hybridisation to confirm localisation of human 5S ribosomal RNA genes.
Two cases of chromosomal translocations involving the long arm of chromosome 1 were investigated for 5S ribosomal gene localisation using in situ hybridisation. In the first family, there was an interstitial translocation of 1q25-32 to chromosome 5; the 5S genes remained on chromosome 1. In the second family, there was a translocation of 1q42-44 to chromosome 21q12; the 5S gene locus in this case was translocated. This shows that the 5S ribosomal genes are at position 1q42-44, confirming a previous assignment based on adenovirus-induced uncoiling and on a partial trisomy (Steffensen et al., 1977).
D002871:Chromosome Banding; D002874:Chromosome Mapping; D002900:Chromosomes, Human, 1-3 / Q000648:ultrastructure; D002904:Chromosomes, Human, 21-22 and Y / Q000648:ultrastructure; D002905:Chromosomes, Human, 4-5 / Q000648:ultrastructure; D005260:Female; D006801:Humans; D006824:Hybridization, Genetic; D008297:Male; D012335:RNA, Ribosomal / Q000235:genetics*; D014178:Translocation, Genetic
D012335:RNA, Ribosomal