A routine screening method for the major metabolite of methyl phenidate in the urine of greyhounds.
A qualitative urine screening procedure for the determination of the major urinary metabolite of methyl phenidate, alpha-phenyl-2-piperidine acetic acid (ritalinic acid), has been devised. The method involves a salting-out extraction, conversion of the metabolite to the parent drug using methanol-hydrogen chloride, and simultaneous two-column gas-chromatographic detection. Final confirmation of the presence of the drug is by two-system thin-layer chromatography. The method is suitable for the detection of illicitly administered methyl phenidate in greyhounds.
D000818:Animals; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004285:Dogs / Q000652:urine*; D004300:Doping in Sports; D008722:Methods; D008774:Methylphenidate / Q000378:metabolism / Q000652:urine*
D008774:Methylphenidate