title: Genome complexities of the three mRNA species of snowshoe hare bunyavirus and in vitro translation of S mRNA to viral N polypeptide.
abstract: The genome complexities of the principal intracellular viral complementary RNA species of the snowshoe hare bunyavirus have been analyzed by duplex analyses involving hybridization of complementary RNA to individual 32P-labeled viral RNA species (large, L; medium, M; and small, S), recovery of nuclease-resistant duplexes, and determination of the oligonucleotide fingerprints of the protected 32P-labeled viral sequences. The result for the M RNA (which codes for the glycoproteins G1 and G2; J. R. Gentsch and D. H. L. Bishop, J. Virol. 30:767-770, 1979) indicates that there is a single polycistronic M mRNA. Similar results were obtained for the L and S RNA species. In vitro translation studies with the S complementary RNA species of snowshoe hare virus as well as melted purified S duplexes substantiate earlier genetic and molecular studies (J. R. Gentsch and D. H. L. Bishop, J. Virol. 28:417-419, 1978; J. Gentsch, D. H. L. Bishop, and J. F. Obijeski, J. Gen. Virol. 34-257-268, 1977), which indicate that S mRNA codes for the virion nucleocapsid protein N.
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000032:analysis*; D002042:Bunyamwera virus / Q000032:analysis* / Q000378:metabolism; D002460:Cell Line; D002474:Cell-Free System; D006224:Cricetinae; D005814:Genes, Viral; D007668:Kidney; D009692:Nucleic Acid Heteroduplexes; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis* / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D009692:Nucleic Acid Heteroduplexes; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D012313:RNA