title: Protein synthesis in skeletal muscle following acute exhaustive exercise.
abstract: Cell-free and whole-tissue protein synthesis was studied in skeletal muscle of untrained male guinea pigs that had undergone a treadmill run to exhaustion. Experiments using explants from the gastrocnemius muscle maintained in organ culture demonstrated that the ability of the acutely exercised muscle to incorporate amino acids into protein had increased. Compared to polyribosomes prepared from several lower hind limb muscles of nonexercised guinea pigs, polyribosomes from the same muscle in exhausted guinea pigs had incorporated almost 50% more radioactive leucine into protein. However, the polysome profiles of control and exercised muscle were identical, and no difference in the total polysome RNA content could be detected. The efficiency of in-vitro protein synthesis using washed membrane-bound polyribosomes (microsomes) isolated from acutely exercised skeletal muscle was 50% greater than with microsomes from rested control muscle.
mesh_terms: D000818:Animals; D002474:Cell-Free System; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000378:metabolism; D009124:Muscle Proteins / Q000096:biosynthesis*; D009132:Muscles / Q000378:metabolism*; D005082:Physical Exertion; D011132:Polyribosomes / Q000378:metabolism
chemical_list: D009124:Muscle Proteins