Early biochemical liver changes following thiobenzamide poisoning.
Administration of thiobenzamide in a single dose (25 mg/100 g body wt by stomach tube) to male rats induced centrilobular necrosis, which became evident 10 h after the poisoning. In the meantime liver weight and water content underwent changes, glycogen was lost, triglycerides accumulated in the liver while decreasing in serum, [3H] leucine uptake in proteins was impaired and the activity of glucose-6-phosphatase and aminopyrine demethylase decreased. The activity of NADPH-cytochrome c reductase remained unchanged, whereas a reduction of the microsomal cytochrome P-450 occurred. The liver amount of reduced glutathione underwent no significant changes. Pretreatment of the animals with cobalt chloride or 20-methylcholanthrene decreased the liver damage caused by the drug. The in vitro addition of thiobenzamide to liver microsomes resulted in a spectral change. The appearance of conjugated dienes among microsomal lipids from drug-treated rats indicated for a lipoperoxidation taking place in vivo.
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001549:Benzamides / Q000506:poisoning*; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D007930:Leucine / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009336:Necrosis; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D013854:Thioamides; D013871:Thiones / Q000506:poisoning; D013997:Time Factors
D001549:Benzamides; D013854:Thioamides; D013871:Thiones; C015539:thiobenzamide; D006899:Mixed Function Oxygenases; D000410:Alanine Transaminase; D007930:Leucine