Effects of sample-diluent interactions on surface tension and pressures in continuous flow manifolds.
When a plasma sample is aspirated into a continuous flow analyser the internal pressure of the manifold increases. The pressure change is the result of insufficient detergent present to bind all the protein in the sample, and is a potential source of inaccuracy and imprecision in the assay. It is recommended that Brij 35 be used at a concentration 10 times above the binding capacity of the albumin in the sample, to minimise changes in pressure between samples.
D001322:Autoanalysis; D001774:Blood Chemical Analysis; D001798:Blood Proteins; D003902:Detergents; D006801:Humans; D011312:Pressure; D011485:Protein Binding; D013499:Surface Properties; D013500:Surface Tension
D001798:Blood Proteins; D003902:Detergents