[Method for assaying methane monooxygenase activity by measuring methane uptake in the reaction vessel].
The reaction vessel has been designed to measure methane monooxygenase activity. An elastic membrane has been built into one of the walls of the vessel to take liquid samples, avoiding formation of the gaseous phase in the reaction volume. The methane content in the samples is measured in a gas-liquid chromatograph with a flame ionization detector in two ways: 1. by direct measurement of methane in the liquid sample, and 2. by measurement of methane in the gaseous phase after methane diffusion from the liquid sample into the gaseous space of another vessel. The method is simple, sensitive (with a lower limit of 0.1 nMole CH4), and well reproducible. This method permits measurement of the oxidation kinetics of methane and other gaseous hydrocarbons both by intact cells and cell-free preparations of methane oxidizing bacteria.
D002849:Chromatography, Gas / Q000379:methods; D019605:Euryarchaeota / Q000378:metabolism; D007700:Kinetics; D008697:Methane / Q000032:analysis*; D008832:Microchemistry; D010105:Oxygenases / Q000378:metabolism*
D010105:Oxygenases; C021555:methane monooxygenase; D008697:Methane
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