A simple, large scale process for purifying human urinary kallikrein, based on reverse osmosis and ammonium sulfate precipitation.
A simple, large scale process for the purification of human urinary kallikrein is described which is based upon concentration by reverse osmosis, ammonium sulfate precipitation, and gel filtration on a column of Sephadex-G-150. The yield in protein is higher than any reported in the literature to this date; the purified enzyme seems to be identical to that reported by Figueiredo and Mares-Guia at the Kinin-Symposium in Paris, 1978, based on the procedure of Hial, et al., (1974).
D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D006801:Humans; D007610:Kallikreins / Q000302:isolation & purification / Q000652:urine*; D007700:Kinetics; D009995:Osmosis
D000596:Amino Acids; D007610:Kallikreins; D000645:Ammonium Sulfate