The virus neutralizing activity of antibodies specific to the envelope and nucleocapsid of equine herpesvirus type 1.
Antibodies specific to the envelope or nucleocapsid of the Kentucky-D strain of equine herpesvirus type 1 were isolated from convalescent horse serum by immunoadsorption on cyanogen bromide-activated Sepharose conjugated with equine herpesvirus type 1 envelopes or nucleocapsids, with subsequent elution by glycine buffer. In double immunodiffusion and immunolectrophoresis reactions, the eluted proteins appeared to belong to the IgG fraction of horse serum. Antibodies directed against the viral envelope neutralized equine herpesvirus type 1 in a plaque neutralization test, while antibodies against the nucleocapsid showed no virus neutralizing activity.
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002213:Capsid / Q000276:immunology*; D006564:Herpesviridae / Q000276:immunology*; D006736:Horses / Q000276:immunology*; D009500:Neutralization Tests
D000914:Antibodies, Viral