Novel opioid peptides derived from casein (beta-casomorphins). I. Isolation from bovine casein peptone.
A material which displayed opioid activity in the guinea pig ileum longitudinal muscle-myenteric plexus preparation was extracted from an enzymatic casein digest into chloroform/methanol. The extract was roughly purified by adsorption/desorption procedures using charcoal and Amberlite XAD-2 resin as adsorbents. A high degree of purity was achieved by high-pressure liquid chromatography of the material on muBondapak C18 and mu-Porasil columns and finally by gel filtration chromatography on a Bio-Gel P-2 column. Several pronase-resistant compounds with opioid activity were obtained.
D000818:Animals; D001681:Biological Assay; D002364:Caseins; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004723:Endorphins / Q000302:isolation & purification* / Q000494:pharmacology; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D010446:Peptide Fragments / Q000302:isolation & purification
D002364:Caseins; D004723:Endorphins; D010446:Peptide Fragments; C021968:beta-casomorphins