Use of the ultracentrifuge vertical rotor in the detection of rubella-specific IgM on a sucrose density gradient.
A comparison was made of the performance of swing-out and vertical ultracentrifuge rotors in the detection of rubella-specific IgM on a sucrose density gradient. Tests were performed on 30 sera, of which 11 were found to contain rubella-specific IgM by both methods. The centrifugation time for the swing-out rotor was 16 hours at 35,000 rpm. This was reduced to 2 hours using the vertical rotor at 50,000 rpm. Routine use of the vertical rotor would allow a reduction in the time taken to perform the test, increase the number of sera tested each time, and reduce wear on the ultracentrifuge.
D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D002499:Centrifugation, Density Gradient / Q000379:methods; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D012409:Rubella / Q000276:immunology; D012412:Rubella virus / Q000276:immunology*; D013997:Time Factors
D000914:Antibodies, Viral; D007075:Immunoglobulin M