Pyruvate dehydrogenase activation in adipocyte mitochondria by an insulin-generated mediator from muscle.
Material in a chromatographic fraction from an extract of insulin-treated muscle stimulated pyruvate dehydrogenase activity in addipocyte mitochondria. This action was similar to insulin's activation of the enzyme in a plasma membrane-mitochondria mixture. Neither the chromatographic fraction nor insulin required adenosine triphosphate or magnesium ion (Mg2+), suggesting that both agents acted through a calcium-sensitive phosphatase. This fraction may contain a chemical mediator of insulin action.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*
D007328:Insulin; D011768:Pyruvate Dehydrogenase Complex; D000255:Adenosine Triphosphate; D008274:Magnesium