Translation of glutamate dehydrogenase mRNA in a reticulocyte cell-free system.
Messenger RNA template activity for glutamate dehydrogenase was detected in poly(A)-rich RNA extracted from rat liver polysomes. Enzyme synthesized in cell-free reticulocyte system was detected by measuring enzyme activity in the translation incubation mixture using dual wavelength spectrophotometric technique. The translation product was also identified by a partial purification of the labeled synthesized enzyme and by coelectrophoresis with the carrier enzyme preparation from mitochondrial matrix.
D000818:Animals; D002474:Cell-Free System; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D013347:Subcellular Fractions
D012333:RNA, Messenger; D005969:Glutamate Dehydrogenase