Total fasting and postprandial serum bile acids determination by 3 alpha-hydroxysteroid dehydrogenase in normal subjects.
Total serum bile acids (SBA) have been evaluated in 28 healthy subjects by an enzymatic-fluorimetric method using the enzyme 3 alpha-hydroxysteroid dehydrogenase. The healthy subjects showed a value of 2.78 +/- 1.84 microM/1 (mean +/- SD), ranging 0.5 to 4.7 microM/1. In addition, two hours postprandial evaluation of SBA was carried out in five of the above subjects. The mean value of SBA before and after the meal was 2.58 +/- 1.4 and 8.73 +/- 1.7 microM/1 respectively and the postprandial increase was found statistically significant (P less than 0.001). The present method for quantitative determination of SBA seems to be sensitive, reliable and useful as a routine clinical aid.
D015096:3-Hydroxysteroid Dehydrogenases; D000293:Adolescent; D000328:Adult; D000368:Aged; D001647:Bile Acids and Salts / Q000097:blood*; D003840:Deoxycholic Acid / Q000097:blood; D004435:Eating; D005215:Fasting; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D001647:Bile Acids and Salts; D003840:Deoxycholic Acid; D015096:3-Hydroxysteroid Dehydrogenases