title: Electrophoretic analyses of lactate dehydrogenase C4 in testes and vesicular glands of normal and male sterile translocation mice.
abstract: Lactate dehydrogenase (LDH) C, activity was observed in testis extracts from normal mice but was progressively reduced in mice carrying the male-sterile translocations T31H, T32H, T37H, T38H, T40H and T42H, with no detectable activity being observed in the last two mice. None of the vesicular gland extracts from these male-steriles showed LDH-C4 activity, unlike normal mice. The differential LDH-C4 activity in male-sterile testes is interpreted as reflecting the varying stages of the spermatogenic defect during meiosis. In general, early meiotic defects exhibited no LDH-C4 activity whereas late stage (usually after metaphase-1 stage) defect animals exhibited some activity. The results also provide evidence for contaminating sperm being the source of normal vesicular gland LDH-C4 activity.
mesh_terms: D000818:Animals; D007248:Infertility, Male / Q000201:enzymology* / Q000235:genetics; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012669:Seminal Vesicles / Q000201:enzymology*; D013094:Spermatozoa / Q000201:enzymology; D013737:Testis / Q000201:enzymology*; D014178:Translocation, Genetic
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase