Purification and characterization of agrocin 84.
A procedure for the rapid purification of milligram quantities of agrocin 84, a bacteriocin-like compound produced by Agrobacterium radiobacter strain K-84, has been developed. This procedure, which employs charcoal adsorption, ion-exchange, sieving chromatography, and continuous-flow electrophoresis, can yield agrocin 84 which is 65% pure on a dry weight basis. The purest preparations were strongly ultraviolet absorbing, with a maximum at 264 nm (epsilon 7.0/264 = 22,675 cm2 - M-1) and a minimum at 227 nm (ratio of 264 to 227 nm, 6.00). As has been reported, agrocin 84 contains an unusual phosphoramidate or 6-N-acyl linkage to adenine. Adenine, glucose, and phosphate are present in a 1:1:2 molar ratio. The molecular weight was estimated to be 1,350.
D000227:Adenine Nucleotides / Q000302:isolation & purification*; D001430:Bacteriocins / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D012231:Rhizobium / Q000378:metabolism*
D000227:Adenine Nucleotides; D001430:Bacteriocins; C013799:agrocin 84