[Role of the components in the methylation system in the cobalamin-dependent gentamycin biosynthesis by a Micromonospora purpurea culture].
It was found that under conditions of a short-term cultivation of the Co-deficient mycelium of M. purpurea var. violaces 1935 in the synthetic medium the level of gentamicin biosynthesis increased on the average by 2--2.5 times when cyancobalamine, methylcobalamine, L-methionine or L-serine were added to the medium. Glycine and H2-pholate increased the gentamicin yield on the average by 1.5--1.8 times. When the concentration of L-methionine in the medium was optimal, production of gentamicin by the Co-deficient mycelium markedly increased on addition of extra amounts of cyancobalamine into the medium. An analogous high level of gentamicin biosynthesis was observed in the absence of L-methionine, when combinations of H2-pholate and L-serine or cyancobalamine, H2-pholate and glycine were added to the medium. The data of the study indicate that the pholate-dependent neogenesis of -CH3 group and the methyl-B12-dependent resynthesis of L-methionine play an important functional role in biosynthesis of gentamicin.
D003035:Cobalt / Q000378:metabolism; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005839:Gentamicins / Q000096:biosynthesis*; D008715:Methionine / Q000096:biosynthesis; D008745:Methylation; D008847:Micromonospora / Q000378:metabolism*; D014805:Vitamin B 12 / Q000378:metabolism*
D003470:Culture Media; D005839:Gentamicins; D003035:Cobalt; D008715:Methionine; D014805:Vitamin B 12
Issledovanie roli nekotorykh komponentov sistemy metilirovaniia v kobalaminzavisimom biosinteze gentamitsina kul'turoi Micromonospora purpurea.