The effect of diphenylhydantoin upon degradation of sulphated macromolecules in cat palatal mucosa in vitro.
The effect of diphenylhydantioin (DPH) upon the degradation of in vivo [35S]-sulphate-labelled proteoglycans was studied in cat palatal mucosa during organ culture. 8-week-old cats were injected intraperitoneally with [35S]-sulphate and 24 hours later the palatal mucosa was taken to organ culture. The release of radioactivity into the culture medium was taken as a measure of degradation of sulphated macromolecules, presumably proteoglycans, and the release of hydroxyproline as an indicator for collagen degradation. A parallel decrease in the release of radioactivity and in the release of hydroxyproline was observed when the culture was done in the presence of DPH (20 mg/l). Chromatography of the culture medium upon Sephadex G-25 revealed that the reduced release of radioactivity was due to a reduction of macromolecular degradation products leaving the amounts of free sulphate in the medium unchanged. The results were interpreted using a two compartment theory for proteoglycan degradation, extracellular breakdown of the protein core resulting in the production of macromolecular degradation products and intracellular lysosomal degradation resulting in free sulphate as the identifiable product. The results indicate that DPH inhibited the extracellular enzymatic degradation of proteoglycans without influencing their intracellular degradation.
D000818:Animals; D002415:Cats; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000378:metabolism; D005260:Female; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D009061:Mouth Mucosa / Q000378:metabolism*; D009924:Organ Culture Techniques; D010160:Palate, Soft / Q000187:drug effects / Q000378:metabolism*; D010672:Phenytoin / Q000494:pharmacology*; D011509:Proteoglycans / Q000378:metabolism*
D011509:Proteoglycans; D010672:Phenytoin; D003094:Collagen; D006909:Hydroxyproline