title: EC-GLC determination of the herbicidal monohalogenated phenoxyalkyl acid mecoprop in tissues, urine and plasma after derivatization with pentafluorobenzylbromide.
abstract: An EC-GLC method for the determination of the systemic herbicide methylchlorophenoxypropionic acid (meco prop), a monohalogenated phenoxyalkyl acid, in 0.5 gram of rabbit tissues and in one ml human urine and plasma, is presented. Monohalogenated chlorophenoxyalkyl acids can reach a high sensitivity for electron-capture detection as pentafluorbenzylester derivatives; so their minimum detectable amount is about 5 pg. A test-tube micro-extraction method for MCPP fromurine, plasma and homogenized tissues with benzene, after acidification with 2 N HC1, the derivatization the residue, and the column clean-up over silicagel, are described in detail. Results of recovery studies with variation-coefficient determination are given by analysis of spiked tissues liver and brain, urine and plasma using p-chlorophenoxy-isobutyric acid (CPIB) as the internal standard. With this method other chlorophynoxyalkyl acid herbicides also can be determined in biological post-mortem material
mesh_terms: D008456:2-Methyl-4-chlorophenoxyacetic Acid / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas; D006016:Glycolates / Q000032:analysis*; D006540:Herbicides / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D008722:Methods
chemical_list: D006016:Glycolates; D006540:Herbicides; D008456:2-Methyl-4-chlorophenoxyacetic Acid