[Comparison of reactivities or uridine- and cytidine-2',3'-cyclophosphates as donors and uridine and cytidine as acceptors of phosphate under dinucleoside monophosphate synthesis catalyzed by RNAase A].
In order to determine the relative activity of pyrimidine nucleoside-2',3'-cyclophosphates as donors and nucleosides as acceptors of phosphate in the reaction of the internucleotide bond formation catalyzed by RNAase A (EC 3.4.1.22), a comparative synthesis of dinucleoside monophosphates UpU, UpC, CpU and CpC at three different enzyme concentrations (20, 40 and 70 mkg/ml) and two temperatures (0 degrees and -15 degrees) was carried out. The conversion rate of donor (U greater than p and C greater than p) during the synthesis and in the competitive reaction of hydrolysis strongly depends on the type of acceptor activity as compared to uridine. Based on the data of synthesis and simultaneous hydrolysis of U greater than p and C greater than p it may be concluded that in the both cases the latter donor is more reactive. The approaches to the determination of the substrate activity of the donors and acceptors for the evaluation of optimal conditions of the dinucleoside monophosphate synthesis depending on the donor--acceptor combination are discussed.
D003562:Cytidine; D003597:Cytosine Nucleotides; D004720:Endonucleases / Q000378:metabolism*; D007700:Kinetics; D009712:Nucleotides, Cyclic; D012260:Ribonucleases / Q000378:metabolism*; D013379:Substrate Specificity; D014500:Uracil Nucleotides; D014529:Uridine
D003597:Cytosine Nucleotides; D009712:Nucleotides, Cyclic; D014500:Uracil Nucleotides; D003562:Cytidine; D004720:Endonucleases; D012260:Ribonucleases; D014529:Uridine
Sravenenie reaktsionnoĭ sposobnosti uridini tsitidin-2',3'-tsiklofosfata kakdonorov, uridina i tsitidina kak aktseptorov fosfata v sinteze dinukleozidmonofosfatov, kataliziruemom RNKazoĭ A.