[Synthesis and properties of insolubilized enzyme. XI. Trypsin-carrier-complexes on the basis of macroporous glasses by interposing the hydrophilic polymer dextran].
A procedure for preparing derivatives of dialdehyde dextran-glass-carriers and their use for immobilization of trypsin is described. The dextran to the support as well as the trypsin to dextran were covalently immobilized via dialdehyde groups. The relative activity of immobilized trypsin in these enzyme-carrier-complexes amounted 17.3% and 20,4 (determined with alpha-N-Benzoyl-DL-arginine-p-nitroanilid) and 8,2% and 11.6% (determined with casein).
D003911:Dextrans; D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D005898:Glass; D008722:Methods; D013379:Substrate Specificity; D014357:Trypsin / Q000378:metabolism
D003911:Dextrans; D004800:Enzymes, Immobilized; D014357:Trypsin
Synthese und Eigenschaften trägerfixierter Enzyme. XI. Trypsin-Träger-Komplexe auf der Basis makroporöser Gläser unter Zwischen-schaltung des hydrophilen Polymers Dextran.