Interrelations between uptake of [14C]orotic acid and labeling of UTP and RNA in rat and mouse liver after partial hepatectomy.
The uptake and utilization of [6-14C]orotic acid for UTP and RNA synthesis were studied in rat and mouse liver at 24 h after partial hepatectomy. Rat liver concentrated radioactivity relative to blood several-fold better than did mouse liver after both sham-operation and partial hepatectomy. The results showed that in mouse liver, contrary to rat liver, the orotic acid uptake was not increased after the partial hepatectomy. In rat liver, the precursor uptake and the labeling of UTP increased by about 75% whereas the specific radioactivity of RNA increased 2 to 3-fold after the operation, thus indicating an increased RNA synthesis. Mouse liver showed no increased [14C]orotic acid uptake or labeling of UTP or RNA at 24 h after partial hepatectomy.
D000818:Animals; D002250:Carbon Radioisotopes; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D051379:Mice; D009963:Orotic Acid / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D013045:Species Specificity; D014500:Uracil Nucleotides / Q000096:biosynthesis*; D014544:Uridine Triphosphate / Q000096:biosynthesis*
D002250:Carbon Radioisotopes; D014500:Uracil Nucleotides; D009963:Orotic Acid; D012313:RNA; D014544:Uridine Triphosphate