The beta-glucuronidase release from macrophages activated by immune complexes of varying antigen/antibody ratio.
When unstimulated rat peritoneal macrophages are exposed in vitro to IC formed with BSA and specific rat anti-BSA IgG antibodies, an exocytosis of the lysosomal beta-G occurs. The maximal release of beta-G into the serum-free medium is induced, without cell lysis, by IC after a 6-h contact with the adherent cell population. This phenomenon is dose-dependent, and the percentage of beta-G in the medium is higher with IC in Ab excess than with other types of IC. In this homologous model (rat macrophages and rat antibodies) the Ag/Ab ratio of IC seems to represent an important factor of macrophage activation.
D000818:Animals; D000936:Antigen-Antibody Complex; D002417:Cattle; D005966:Glucuronidase / Q000378:metabolism*; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology; D051381:Rats
D000936:Antigen-Antibody Complex; D005966:Glucuronidase