Carbohydrates of influenza virus. III. Nature of oligosaccharide-protein linkage in viral glycoproteins.
Four different glycopeptides can be distinguished after pronase digestion of influenza A virus glycoproteins: Ia and Ib, containing N-acetylglucosamine, mannose, galactose, and fucose, and IIa and IIb, containing mannose and N-acetylglucosamine. All glycopeptides yielded N-acetylglucosaminyl-asparagine after mild acid hydrolysis. There was no evidence for O-glycosidic bonds. Thus, the carbohydrate complement is linked to the polypeptide exclusively by N-glycosidic linkages between N-acetylglucosamine and asparagine.
D000117:Acetylglucosamine / Q000032:analysis; D001216:Asparagine / Q000032:analysis; D002236:Carbohydrate Conformation; D006020:Glycopeptides / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D009980:Influenza A virus / Q000032:analysis*; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis; D011487:Protein Conformation; D014764:Viral Proteins / Q000032:analysis*
D006020:Glycopeptides; D006023:Glycoproteins; D009844:Oligosaccharides; D014764:Viral Proteins; D001216:Asparagine; D000117:Acetylglucosamine