Granulocyte progenitor cells (CFU-C) in peripheral blood of normal volunteers following cell collection by continuous flow centrifugation.
During leukapheresis, the donor undergoes cellular depletion which must be compensated. Moreover, the cellular suspensions obtained contain a certain proportion of granulocyte progenitors that form colonies in agar (CFU-C). These findings prompted us to investigate the effect of a donation by cytapheresis on the number of circulating CFU-C present in the donor. Our investigations were carried out on the day the donation was made, and at further fixed dates for a period of six months. Our study covers 25 cases, corticoid premedication was only given to granulocyte donors and not to platelet donors. In the majority of cases, there were no postdonation effects. A few donors had a less stable circulating CFU-C count than is generally considered normal for a healthy subject. In some, quite wide variations were observed but immature cells never appeared in the circulating blood.
D001792:Blood Platelets; D003114:Colony-Forming Units Assay; D006098:Granulocytes; D006801:Humans; D007937:Leukapheresis