Methods for the determination of glandular kallikrein by means of a chromogenic tripeptide substrate.
A chromogenic peptide substrate H-D-Val-Leu-Arg-pNA (S-2266) has been used for the determination of glandular kallikrein derived from pancreas, urine and saliva. The conditions used have been optimized. The methods developed are simple and shown to have good reproducibility.
D006801:Humans; D007610:Kallikreins / Q000032:analysis* / Q000652:urine*; D007700:Kinetics; D009842:Oligopeptides; D010179:Pancreas / Q000201:enzymology*; D012463:Saliva / Q000201:enzymology*; D013053:Spectrophotometry / Q000379:methods
D009842:Oligopeptides; D007610:Kallikreins