Purification and the histones of Dictyostelium discoideum chromatin.
Dictyostelium chromatin has been purified from nuclei in high yield by differential centrifugation and nuclease cleaving. Its chemical composition has been assayed, and its histones have been analyzed by gel electrophoresis, peptide fingerprints, amino acid composition, and ion-exchange chromatography. The mass ratios of DNA/RNA/histone/nonhistone are 1.0:0.18:0.98:1.02. There are four histones including one unusual histone, H7, which is the most abundant histone in the slime mold. The H4-like protein is the most conserved protein, while the other histones show both similarities and differences with mammalian histones.
D000596:Amino Acids / Q000032:analysis; D002458:Cell Fractionation; D002843:Chromatin / Q000032:analysis* / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D004247:DNA / Q000032:analysis; D004023:Dictyostelium / Q000032:analysis*; D006657:Histones / Q000302:isolation & purification; D010446:Peptide Fragments / Q000032:analysis; D012313:RNA / Q000032:analysis
D000596:Amino Acids; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D010446:Peptide Fragments; D012313:RNA; D004247:DNA