Purification of the JS-3 isolate of Herpesvirus ovis (Bovid herpesvirus 4) and some properties of its DNA.
A procedure incorporating the use of heparin was developed to purify Herpesvirus ovis. The viral DNA has a buoyant density of 1.706 +/- 0.001 g/cm3, and the sedimentation constant was estimated to be 47.5 +/- 1.5S; from the latter, the molecular weight was calculated as 67.3 +/- 5.4 X 10(6). Estimates of the guanine-plus-cytosine content made from the buoyant density and melting point (72 degrees C) gave levels of 47 and 46%, respectively.
D002499:Centrifugation, Density Gradient; D003596:Cytosine / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis*; D006147:Guanine / Q000032:analysis; D006564:Herpesviridae / Q000032:analysis / Q000302:isolation & purification*; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation
D004279:DNA, Viral; D006147:Guanine; D003596:Cytosine