[Biosynthesis of glycoconjugates in the arterial intima. VII. Isolation and purification of fucosyl-transferase from the aorta].
A fucosyl-transferase, catalysing the transfer of fucose, from GDP-fucose to proteinic or polyprenic acceptors, exists in microsomes of intima-media from aorta. Solubilization and purification of this enzyme is obtained by treatment of microsomes with Cemulsol NPT 12, column chromatography on DEAE-cellulose, Dowex 1 X 8 and electrofocusing. Only one molecular species is detected at pHi = 4.7.
D000818:Animals; D001011:Aorta / Q000201:enzymology*; D002417:Cattle; D005647:Fucosyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D006154:Guanosine Diphosphate Fucose; D006602:Hexosyltransferases / Q000302:isolation & purification*; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D012995:Solubility; D013501:Surface-Active Agents
D013501:Surface-Active Agents; D006154:Guanosine Diphosphate Fucose; D005647:Fucosyltransferases; D006602:Hexosyltransferases
Biosynthèse des glycoconjugués dans la paroi artérielle. VII. Isolement et purification de la fucosyl-transférase de l'aorte.