[Fragmentation of the DNA of simian adenovirus type 38 by using restriction endonucleases].
The effect of restricting endonucleases Eco R I, BgI II and Sal I on simian adenovirus type 38 (SV-38) DNA was studied. Bgl II restrictase cleaves the virus DNA into 4 fragments, A, B, G, and D, with molecular weights 9.3 x 10(6), 3.3 x 10(6), and 2.9 x 10(6) daltons, respectively. After restriction with Eco R I and Sal I SV-38 DNA cleaves into 5 and 6 fragments, respectively. The molecular weights of Eco R I fragments are A--8.2 x 10(6), B--6.5 x 10(6), C--4.0 x 10(6), D--1.27 x 10(6), and of Sal I fragments: A--6.5 x 10(6), B--5.4 x 10(6), C--4.2 x 10(6), D--2.8 x 10(6), E--2.5 x 10(6), and F--0.25 x 10(6). By restriction of DNA-protein compex by means of partial DNA hydrolysis, combined Bgl II and Eco R I hydrolysis and secondary restriction of a fragment eluted from the agar gel, the alternation of Eco R I and Bgl II fragments in SV-38 genome was determined.
D000256:Adenoviridae / Q000187:drug effects*; D000261:Adenoviruses, Simian / Q000187:drug effects*; D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005814:Genes, Viral / Q000187:drug effects; D006868:Hydrolysis; D008970:Molecular Weight
D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Fragmentatsiia DNK adenovirusa obez'ian tipa 38 s pomoshch'iu restriktiruiushchikh éndonukleaz.