Production and isolation of chitosan from Mucor rouxii.
A method for the lab-scale production and isolation of chitosan (polyglucosamine) from hyphal walls of Mucor rouxii was developed. Hyphal wall yields were generally 16 to 22% on a dry cell weight basis, of which 35 to 40% was glucosamine. Chitosan was readily extracted from purified, mycelial walls with acetic, formic, and hydrochloric acids; the last named was the most efficient. The yield of chitosan isolated ranged from 4 to 8% of the dry weight of the cell wall material.
D002473:Cell Wall / Q000032:analysis; D003513:Cycloheximide / Q000494:pharmacology; D005944:Glucosamine / Q000096:biosynthesis / Q000302:isolation & purification; D005947:Glucose / Q000378:metabolism; D009089:Mucor / Q000032:analysis / Q000378:metabolism*; D011108:Polymers; D011134:Polysaccharides / Q000096:biosynthesis*
D011108:Polymers; D011134:Polysaccharides; D003513:Cycloheximide; D005947:Glucose; D005944:Glucosamine