Simultaneous assay for L-tryptophan, serotonin, 5-hydroxyindoleacetic acid, norepinephrine and dopamine in brain.
A routine simultaneous assay for the title compounds is described, which uses a cation-exchange resin for separations, and standard fluorometric methods for analyses. Practicability of the ion-exchange chromatography is enhanced by means of a novel apparatus, and the procedure has the flexibility to permit extension to other endogenous compounds using published techniques.
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D004298:Dopamine / Q000032:analysis*; D006897:Hydroxyindoleacetic Acid / Q000032:analysis*; D008094:Lithium / Q000494:pharmacology; D008297:Male; D008722:Methods; D009638:Norepinephrine / Q000032:analysis*; D051381:Rats; D012701:Serotonin / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000032:analysis*
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D014364:Tryptophan; D008094:Lithium; D004298:Dopamine; D009638:Norepinephrine