Antileukocyte antibodies in patients refractory to platelet transfusions.
Sera from 12 multitransfused patients who were refractory to random-donor platelets were tested for lymphocytotoxic and leukoagglutinating antibodies using panel cells from various volunteers whose HLA-A and -B antigens were known. All sera contained leukoagglutinins reactive with cells from at least one panel member, whereas only 33% had lymphocytotoxic antibodies. Patients whose sera reacted frequently with panel cells using the microcapillary agglutination technic seldom responded to HLA-matched paltelets, whereas those whose sera reacted infrequently usually responded satisfactorily. It is concluded that non-HLA antibodies may play a significant role in determining the responses to platelet transfusions in multi-transfused patients.
D000372:Agglutination Tests; D000906:Antibodies; D001792:Blood Platelets / Q000276:immunology*; D001803:Blood Transfusion; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D010983:Plateletpheresis
D000906:Antibodies; D006680:HLA Antigens