[Use of liposomes for incorporation of alkylating derivatives of mono- and oligonucleotides into mammalian cells].
It was shown that within the liposomes mono- and oligonucleotides and their alkylating derivatives penetrate the cells of Ehrlich ascite carcinoma and peritoneal exudate of the mice. Inside the cells the alkylating reagents are mainly utilized for modification of proteins (42--76%), RNA (5--16%) and DNA (3--9%). Presumably DNA modification is largely dependent on the penetration of the reagents into the nuclei. No significant differences in alkylation of the cell components by oligoadenylate derivatives, capable of complementary interactions with nucleic acids and mononucleotide derivatives, incapable of such interactions, were observed.
D000477:Alkylating Agents / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D007700:Kinetics; D008081:Liposomes; D051379:Mice; D009711:Nucleotides / Q000378:metabolism*; D009841:Oligonucleotides / Q000378:metabolism*; D013329:Structure-Activity Relationship
D000477:Alkylating Agents; D008081:Liposomes; D009711:Nucleotides; D009841:Oligonucleotides
Ispol'zpvanie liposom dlia vvedeniia alkiliruiushchikh proizvodnykh monoi oligonukleotidov v kletki mlekopitaiushchikh.