Circulating immune complexes in cutaneous vasculitis. Detection with C1q and monoclonal rheumatoid factor.
To investigate the pathogeneic significance of immune complexes in cutaneous vasculitis, 107 patients with various forms of cutaneous vasculitis, including 59 patients with necrotizing (leukocytoclastic) vasculitis (group 1), and 48 patients with lymphocytic vasculitis, or a predominately lymphocytic perivascular infiltrate (group 2), were studied. Immunoglobulins or complement components in cutaneous blood vessels were detected by direct immunofluorescence in high frequency in both groups (91 and 88%, respectively). Using two radioassays for circulating immune complexes, Clq or monoclonal rheumatoid factor (mRF) reactive material was detected in 68% of the patients with necrotizing vasculitis but only 44% of the patients in the lymphocytic-perivascular group. The mRF radioassay was elevated in 58% of the first group of patients and 41% of the patients in group 2, although Clq binding activity was increased in 54% of the patients with necrotizing vasculitis but only in 9% of the patients with a lymphocytic vasculitis or lymphocytic perivascular infiltrate. By using both sucrose density gradient ultracentrifugation and Sepharose 6B gel filtration, the Clq and mRF reactive material detected in some patients with necrotizing vasculitis eluted in high molecular weight fractions that were also anticomplementary. In one patient with necrotizing vasculitis and hepatitis B antigenemia, these heavy molecular weight Clq and mRF reactive fractions contained a two- to three-fold increase in hepatitis B surface antigen when compared with lighter molecular weight fractions. Heavy and light molecular weight mRF reactive material could be detected in selected patients in the lymphocytic-perivascular group as well as in the necrotizing vasculitis group. These studies suggest that cutaneous vasculitis, including acute necrotizing (leukocytoclastic) vasculitis and some forms of lymphocytic vasculitis, and perhaps some diseases characterized by a lymphocytic perivascular infiltrate, may represent cutaneous expressions of immune complex disease.
D000936:Antigen-Antibody Complex; D001681:Biological Assay; D003172:Complement C1 / Q000378:metabolism*; D003176:Complement C3 / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism; D003450:Cryoglobulins / Q000378:metabolism; D005337:Fibrin / Q000378:metabolism; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism; D012217:Rheumatoid Factor / Q000037:antagonists & inhibitors*; D018366:Vasculitis, Leukocytoclastic, Cutaneous / Q000276:immunology* / Q000378:metabolism
D000936:Antigen-Antibody Complex; D003172:Complement C1; D003176:Complement C3; D003450:Cryoglobulins; D007075:Immunoglobulin M; D005337:Fibrin; D003165:Complement System Proteins; D012217:Rheumatoid Factor