[Biosynthesis of glycoconjugates in the arterial wall. VI. Purification and molecular mechanism of N-acetylglucosaminyl transferase].
Aortic intima N-acetyl-glucosaminyl-transferase has been purified by chromatography and isoelectric focusing, allowing her resolution into two insoenzymes, GN 1 (pHi = 4.2) and GN 2 (pHi = 8.5). The phenomenological analysis of this isoenzymes, involving two substrates and two products, showes one mechanism: Bi-Bi random.
D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000201:enzymology*; D001158:Arteries / Q000201:enzymology; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification; D008970:Molecular Weight
D007527:Isoenzymes; D005964:Glucosyltransferases; D000117:Acetylglucosamine
Biosynthèse des glycoconjugués dans la paroi artérielle. VI. Purification et mecanisme moleculaire de la n-acetyl-glucosaminyl transferase.