Effect of phosphatidylcholine vesicles on the activity of DNA polymerase-alpha.
Phosphatidylcholine vesicles stimulate the activity of the DNA polymerase-alpha from calf thymus. This effect is dependent upon the way of addition to the Mg ions, and the extent of the 3H-dTTP incorporation is closely related to the concentration of the vesicles. A role of phospholipids on the activity of the DNA-related enzymes is suggested.
D000818:Animals; D002417:Cattle; D004257:DNA Polymerase II / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008567:Membranes, Artificial; D010713:Phosphatidylcholines / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000378:metabolism; D013950:Thymus Gland / Q000201:enzymology*
D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D013942:Thymine Nucleotides; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase; D008274:Magnesium