Metrizamide gradient purification of mouse tumor cells.
A one-step isopycnic density gradient centrifugation procedure is described for purification of highly viable and homogeneous tumor cells from a variety of solid mouse tumors. Mechanically suspended cells are layered onto preformed continuous gradients of medium 199-buffered 7--33% metrizamide (density range 1.05--1.20 g/cu. cm) isoosmotic with mouse plasma and centrifuged for 30 min. Large numbers of tumor cells, generally 85--95% viable and free from 80--95% of contaminating host lymphoid and phagocytic cells and erythrocytes, were consistently recovered from fractionated thymomas, melanomas, and fibrosarcomas. By a variety of criteria, cell surface and other biological properties of gradient-purified tumor cells were normal.
D000818:Animals; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic; D002499:Centrifugation, Density Gradient; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology; D008175:Lung Neoplasms / Q000556:secondary; D008214:Lymphocytes / Q000276:immunology; D008545:Melanoma / Q000276:immunology; D008793:Metrizamide / Q000494:pharmacology*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology; D010586:Phagocytes / Q000276:immunology; D013945:Thymoma / Q000276:immunology
D008793:Metrizamide