Distribution of lymphocyte messenger RNA during stimulation by phytohaemagglutinin.
Additional of phytohaemagglutinin to cultured lymphocytes results in a progressive increase in the rate of protein synthesis of up to 7-10 times the initial rate after 20 h. Between 2 h and 12 h after the addition of mitogen, the increase in the rate of protein synthesis can be accounted for by the transfer of mRNA from messenger ribonucleoprotein complexes to polysomes. Between 12 h and 20 h the increase also reflects the accumulation of mRNA and ribosomes. However, the proportion of mRNA associated with ribosomes in unstimulated lymphocytes is unexpectedly high and paradoxically decreases during the first 2 h after mitogen addition, although the rate of protein synthesis increases. A mechanism involving mRNA selection is suggested.
D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis; D037102:Lectins; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011072:Poly U / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013552:Swine
D001798:Blood Proteins; D037102:Lectins; D012333:RNA, Messenger; D011072:Poly U