Application of electroimmunoassay to the study of plasma protein synthesis in cultured hepatocytes.
Electroimmunoassay has been applied to the study of plasma protein synthesis and secretion in liver cell cultures. The assay is performed on unconcentrated samples of culture medium containing the secreted plasma proteins and yields results within 2 hours. The characteristics of plasma protein production by the cultured hepatocytes coupled with the sensitivity of this assay permit the study of plasma protein in synthesis and its regulation by hormones and other agents without the routine use of radioisotopes.
D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis*; D002478:Cells, Cultured; D002642:Chick Embryo; D003378:Counterimmunoelectrophoresis; D007122:Immunoelectrophoresis; D008099:Liver / Q000378:metabolism*
D001798:Blood Proteins