The effects of decreased glucose concentrations on the in vitro development of the post-blastocyst mouse embryo in a fetal calf serum- or bovine serum albumin-supplemented medium.
Decreasing the glucose concentration from 1.0 mg/ml to 0.25 mg/ml has no detrimental effects on post-blastocyst embryo development when either dialyzed fetal calf serum serum (20%) or bovine serum albumin (4.0 mg/ml) is used to supplement Eagle's Basal Medium (BME). Development is reduced in both serum- and BSA-supplemented BME devoid of glucose in comparison to glucose controls. Serum-supplemented media support better overall development than BSA-supplemented media.
D000818:Animals; D001769:Blood; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media; D004622:Embryo, Mammalian / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D051379:Mice; D011247:Pregnancy; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D003470:Culture Media; D012710:Serum Albumin, Bovine; D005947:Glucose