Dopamine-4-O-sulfate: a possible precursor of free norepinephrine.
Incubation of dopamine-4-O-sulfate with purified bovine dopamine-beta-hydroxylase led to the formation of free norepinephrine. The production of free norepinephrine was completely inhibited in the presence of 5.6 mM of fusaric acid, an inhibitor of dopamine-beta-hydroxylase. No sulfatase activity was detected in the incubation medium. The reaction of dopamine-4-O-sulfate with dopamine-beta-hydroxylase followed Michaelis-Menten kinetics, showing an apparent Km of 2.6 mM.
D000311:Adrenal Glands / Q000201:enzymology; D000818:Animals; D002417:Cattle; D004298:Dopamine / Q000378:metabolism*; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D005669:Fusaric Acid / Q000494:pharmacology; D007700:Kinetics; D009638:Norepinephrine / Q000096:biosynthesis*; D013379:Substrate Specificity; D013464:Sulfuric Acids / Q000378:metabolism
D013464:Sulfuric Acids; D004299:Dopamine beta-Hydroxylase; D005669:Fusaric Acid; D004298:Dopamine; D009638:Norepinephrine