Multilayer immobilized-enzyme filter reactors: urease bound to nylon fabric filters.
Urease was bound to commercially available nonwoven nylon fabric filters. Multilayer immobilized-enzyme filter reactors were constructed by packing varying numbers of urease-nylon filters in a column. Owing to the relatively open structure and high mechanical strength of the filter fabric, compaction and pressure drop effects were minimal. The reactors could be operated in a wide range of substrate concentrations and flow rates under conditions where mass-transfer limitations could be neglected. The kinetic behavior of the immobilized-enzyme filter reactors could be described by a linear form of the integrated Michaelis-Menten equation using a model based on the sequential action of the enzyme filters.
D000178:Acrylamides; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005374:Filtration / Q000295:instrumentation*; D007700:Kinetics; D008956:Models, Chemical; D009757:Nylons; D013784:Textiles; D014510:Urease / Q000378:metabolism*
D000178:Acrylamides; D004800:Enzymes, Immobilized; D009757:Nylons; D014510:Urease