Composition and molecular organization of lipids and proteins in the envelope of mycoplasmavirus MVL2.
MVL2 virus was purified from culture supernatants of infected Acholeplasma laidlawii cells by differential centrifugation, followed by velocity centrifugation in sucrose gradients. The purified virus contained 0.08 to 0.1 mumol of lipid phosphorous per ml of viral protein. Thin-layer chromatography of viral lipids revealed the presence of phospho-, glyco-, and phosphoglycolipids identical with those found in the host cell membrane, but the relative amount of phosphatidylglycerol was much lower than that in the virus. The fatty acid composition of lipids incorporated into the virus included lipids synthesized before and after infection. The freedom of motion of spin-labeled fatty acids in MVL2 depended markedly on temperature and on the position of the nitroxide group on the hydrocarbon chain of the probe, suggesting that the local environment of the probe has the properties of a lipid bilayer. Nevertheless, the lipid hydrocarbon chains in MVL2 appear to be less mobile than those in membranes of the host cells. Polyacrylamide gel electrophoresis of purified MVL2 revealed four major and about five minor polypeptide bands. None of the polypeptide bands gave a positive periodic acid-Schiff reaction. Lactoperoxidase-mediated iodination, followed by proteolytic digestion of intact MVL2 particles, revealed that at least two major polypeptides are localized on the external surface of the viral envelope.
D000128:Acholeplasma laidlawii; D001435:Bacteriophages / Q000032:analysis* / Q000302:isolation & purification; D002462:Cell Membrane / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis*
D005227:Fatty Acids; D006017:Glycolipids; D008055:Lipids; D010743:Phospholipids; D014764:Viral Proteins