Electrophoretic separation of A gamma and G gamma human globin chains in Nonidet P-40.
Electrophoresis in cellulose acetate in the presence of 3% Nonidet P-40 can resolve two neutral genetic variants, A gamma and G gamma human fetal globin chains. The ratio of these two chains, determined by densitometry of the electrophoretic strips, is in excellent agreement with the Gly-Ala ratio obtained by chemical analysis of the cyanogen bromide fragment gamma CB3. It is suggested that the detergent binds preferentially to the hydrophobic amino acid segment 133-141 in the A gamma chain, thus masking either a Lys or an Arg residue at the two extremes.
D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D005319:Fetal Hemoglobin; D014644:Genetic Variation; D005914:Globins / Q000302:isolation & purification; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D046911:Macromolecular Substances; D017830:Octoxynol; D011092:Polyethylene Glycols
D046911:Macromolecular Substances; D011092:Polyethylene Glycols; D017830:Octoxynol; D005914:Globins; D005319:Fetal Hemoglobin; C010615:Nonidet P-40