The effect of morphine tolerance on the incorporation of 3H-leucine into proteins of rat synaptic membranes.
The rate of incorporation of 3H-leucine into proteins of the synaptic junctional and nonjunctional membranes and synaptic vesicles of rat brain has been examined in control and morphine-tolerant rats. There are no discernible differences between control and tolerant animals in amount of protein as measured by densitometric tracings of dye-stained proteins separated by acrylamide gel electrophoresis from the three membrane fractions of whole brain areas. However, there are differences in the turnover of membrane protein: three vesicle protein bands and one junctional-membrane protein band are significantly more highly labeled, and one junctional-membrane protein is significantly less highly labeled by 3H-leucine in samples from tolerant rats. Of these, the two junctional-membrane proteins can be tentatively identified as components of the post synaptic densities, and one of the vesicle proteins as tubulin.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004361:Drug Tolerance; D007930:Leucine / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D009020:Morphine / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D051381:Rats; D013570:Synaptic Membranes / Q000187:drug effects / Q000378:metabolism*
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009020:Morphine; D007930:Leucine