An improved microchromatographic procedure for separating HbA2 from HbS for the purpose of quantitation.
A microchromatographic method for separating hemoglobin A2 from hemoglobin S and their subsequent quantitation on DEAE-cellulose has been improved. The new procedure is more versatile, since the same system can be used when hemoglobin S is present or absent in the sample without changing to a longer column or another buffer system. Also, the technic is less sensitive to small changes in pH of buffers or resin than are other procedures.
D000755:Anemia, Sickle Cell / Q000175:diagnosis; D002848:Chromatography, DEAE-Cellulose / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006441:Hemoglobin A / Q000032:analysis*; D006451:Hemoglobin, Sickle / Q000032:analysis*; D006801:Humans; D012805:Sickle Cell Trait / Q000175:diagnosis; D013789:Thalassemia / Q000175:diagnosis
D006451:Hemoglobin, Sickle; D006441:Hemoglobin A