title: Polymorphism of the migration of double-stranded RNA genome segments of reovirus isolates from humans, cattle, and mice.
abstract: A series of 94 isolates of reovirus from humans, cattle, and mice, showed extensive variability in the patterns of migration of the ten double-stranded RNA genome segments. This variation was found in all three serotypes, and involved all ten genome segments, including the segment responsible for serological specificity. Although a single pattern was present among several samples isolated from individuals and collected at a single time and place, there were often multiple genetic variants of a single serotype present in a population. Samples isolated from widely different geographic origins or different mammalian hosts showed different patterns; samples from a single species from the same area over a period of time showed more limited variations. Among most isolates, the migration of the slowest S segment, the segment that encodes the hemagglutinin and is responsible for serological specificity in laboratory strains, was similar to reference strains for type 1 and type 3 isolates. However, the type 2 isolates showed considerable variation in this segment.
mesh_terms: D001003:Anal Canal / Q000382:microbiology; D000818:Animals; D002417:Cattle / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D005814:Genes, Viral; D006801:Humans; D012089:Mammalian orthoreovirus 3 / Q000032:analysis; D051379:Mice / Q000382:microbiology; D010614:Pharynx / Q000382:microbiology; D011110:Polymorphism, Genetic; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012087:Reoviridae / Q000032:analysis*
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral