A modified quantitative precipitin test which is rapid, sensitive and reproducible.
A modification of the standard quantitative precipitin test is described. The new procedure, which utilizes polyethylene glycol (PEG) at both the 37 degrees C and 4 degrees C incubation stages, provides a convenient quantitative precipitin test assay which is more reliable and much faster than the standard assay. Moreover, the modified or PEG assay gives a quantitative measure of antibody concentration even when antisera of low titre are tested. The sensitivity of the capillary-tube test is also enhanced when the supernatant solutions contain PEG.
D000818:Animals; D000936:Antigen-Antibody Complex; D001667:Binding, Competitive; D002417:Cattle; D006241:Haptens / Q000276:immunology; D011092:Polyethylene Glycols; D011233:Precipitin Tests / Q000379:methods*; D011234:Precipitins / Q000276:immunology; D011817:Rabbits; D012015:Reference Standards; D012995:Solubility; D013696:Temperature; D013997:Time Factors
D000936:Antigen-Antibody Complex; D006241:Haptens; D011234:Precipitins; D011092:Polyethylene Glycols