The subcellular distribution and some properties of the cytochrome b component of the microbicidal oxidase system of human neutrophils.
A novel cytochrome b has recently been described in human neutrophils. The subcellular distribution of this cytochrome was investigated by analytical fractionation on continuous sucrose gradients and it was found to have a dual localization, the major component having a similar distribution to the plasma-membrane marker [3H]concanavalin A, and a denser peak located with the specific granules. The two components were separated on discontinuous gradients and studied independently.
D002499:Centrifugation, Density Gradient; D002500:Centrifugation, Isopycnic; D003208:Concanavalin A; D003580:Cytochromes / Q000097:blood*; D006801:Humans; D009504:Neutrophils / Q000201:enzymology*; D010088:Oxidoreductases / Q000097:blood; D010587:Phagocytosis; D013053:Spectrophotometry; D013347:Subcellular Fractions / Q000201:enzymology
D003580:Cytochromes; D003208:Concanavalin A; D010088:Oxidoreductases