High-pressure liquid chromatographic determination of propylthiouracil in human plasma.
A high-pressure liquid chromatographic procedure was developed for the determination of prophylthiouracil in plasma. The method utilizes a reversed-phase C18 chromatographic column and UV detection at 280 nm. The method is sufficiently sensitive for most bioavailability and pharmacokinetic studies. Coinentrations between 0.1 and 5.0 microgram/ml were measured with a coefficient of variation of 0.6-5.3% for a given day. Methylthiouracil was employed as an internal standard.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008722:Methods; D011441:Propylthiouracil / Q000097:blood*
D011441:Propylthiouracil