An insert for the reorienting gradient rotor for virus extraction from infected plants.
A device made of nylon and which is inserted in the bowl of the reorienting gradient rotor is described. This insert acts as a container for holding infected plant tissues for the purpose of separating virus by centrifugal force from intact and fresh plants, from frozen and thawed plants and from plant tissue disintegrated by mechanical means. The extracted fluid represents 80 to 90% of that present in the untreated plant tissue. Electron micrographs taken of concentrates of the viruses prepared by the centrifugation extraction procedure indicate that extraneous materials are reduced to a low level.
D002499:Centrifugation, Density Gradient / Q000295:instrumentation* / Q000379:methods; D005615:Freezing; D009029:Mosaic Viruses / Q000302:isolation & purification; D010944:Plants / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000302:isolation & purification