Induction of aryl hydrocarbon hydroxylase in primary cultures of type II alveolar lung cells and binding of metabolically activated benzo[a]pyrene to nuclear macromolecules.
Alveolar-like structures cultured on a gelatin sponge substrate are composed of cells resembling type II alveolar pneumonocytes. These cells contain aryl hydrocarbon hydroxylase (AHH) as determined by fluorescent measurements of water-soluble product from the metabolism of polycyclic aromatic hydrocarbons (PAH). Of 3 compounds tested, benzo[a]pyrene (BP) stimulates the highest level of AHH activity. Activity reaches a peak in 6 day cultures and remains relatively stable for a culture period of 12 days. Metabolites of tritium labeled BP interact with nuclear macromolecules in these cells as determined by measurements of 3H binding to DNA, histone and non-histone chromosomal proteins. A preferential binding to the non-histone protein fraction occurs.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis*; D001580:Benzopyrenes / Q000378:metabolism / Q000494:pharmacology*; D001711:Biotransformation; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D009698:Nucleoproteins / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000378:metabolism; D051381:Rats
D001580:Benzopyrenes; D009698:Nucleoproteins; D004247:DNA; D001189:Aryl Hydrocarbon Hydroxylases