Evaluation of methods for estimating the dissociation constant of tight binding enzyme inhibitors.
D000818:Animals; D002645:Chickens; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005493:Folic Acid Antagonists; D006801:Humans; D008099:Liver / Q000201:enzymology; D008433:Mathematics; D008722:Methods; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D009374:Neoplasms, Experimental / Q000201:enzymology; D011485:Protein Binding; D051381:Rats; D012513:Sarcoma, Experimental / Q000201:enzymology
D004791:Enzyme Inhibitors; D005493:Folic Acid Antagonists; D008727:Methotrexate