Studies on the penetration of mammalian cells by deoxyribonucleoside-5'-phosphates.
We have tested the ability of [5'-32P]-deoxyribonucleoside monophosphates (dNMPs) to penetrate living mouse fibroblast L cells and human HeLa cells. Under the conditions of our experiments, small numbers of apparently intact dNMP molecules appeared to penetrate into the interior of L cells and be incorporated into DNA. This incorporation was not due to mycoplasma contamination nor to extracellular hydrolysis of the dNMPs followed by resynthesis inside the cell. Under these same conditions, penetration of HeLa cells by intact dNMPs did not occur to a significant extent. However, HeLa cells were capable of hydrolyzing extracellular dNMPs to Pi and deoxyribonucleosides at a much faster rate than L cells. These experiments provide a starting point for attempts to specifically label the DNA in intact, living eukaryotic cells with [32P]-dNMPs.
D000818:Animals; D002463:Cell Membrane Permeability; D003854:Deoxyribonucleotides / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007739:L Cells / Q000378:metabolism; D051379:Mice; D010761:Phosphorus Radioisotopes; D013938:Thymidine Monophosphate / Q000378:metabolism
D003854:Deoxyribonucleotides; D010761:Phosphorus Radioisotopes; D013938:Thymidine Monophosphate