Rapid development of immunoprecipitins in agarose gel.
A procedure for the rapid development of immunoprecipitins in agarose gels has been developed using polyanion precipitation after immunoelectrophoretical separations. Excellent results are obtained for serum apolipoproteins, immunoglobulins and other proteins. It is shown that quantitative determination of apolipoprotein B in hyperlipemic serum and isolated lipoproteins is affected by immunoprecipitated complexes when rocket immunoelectrophoresis is used. This effect can be avoided by addition of polyvinylpyrrolidone.
D001053:Apolipoproteins / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D007070:Immunoglobulin A / Q000032:analysis; D008074:Lipoproteins / Q000032:analysis; D008345:Manganese; D010772:Phosphotungstic Acid; D011205:Povidone; D011234:Precipitins / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis
D001053:Apolipoproteins; D001798:Blood Proteins; D007070:Immunoglobulin A; D008074:Lipoproteins; D011234:Precipitins; D012709:Serum Albumin; D010772:Phosphotungstic Acid; D008345:Manganese; D011205:Povidone