An enzyme-polymer film prepared with the use of poly(vinyl alcohol) bearing photosensitive aromatic azido groups.
Photochemical reaction of poly(vinyl alcohol) bearing aromatic azido groups was applied for immobilization of beta-glucosidase (beta-D-glucoside glucohydrolase, EC 3.2.1.21.) in poly(vinyl alcohol) film. Photo-crosslinking and immobilization reactions proceeded by light irradiation for 25 min in air. The immobilized enzyme showed approx. 40% of its native enzyme activity with an apparent Michaelis constant of 3.9 mM. The Michaelis constant of the native enzyme was 2.3 mM. Some properties of the immobilized and native enzyme are compared.
D001386:Azides; D001565:Benzoates; D004800:Enzymes, Immobilized; D005959:Glucosidases / Q000378:metabolism; D007700:Kinetics; D010777:Photochemistry; D011142:Polyvinyl Alcohol
D001386:Azides; D001565:Benzoates; D004800:Enzymes, Immobilized; D011142:Polyvinyl Alcohol; D005959:Glucosidases