Purification of 2-deoxy-2-dansylamido-D-glucose by affinity chromatography on a lectin-loaded agarose column.
A method is demonstrated to purify 2-substituted derivatives of glucose and sterically related sugars by affinity chromatography on Con A-sepharose. The method seems to be of rather general applicability.
D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A; D003619:Dansyl Compounds / Q000302:isolation & purification*; D005944:Glucosamine / Q000302:isolation & purification*; D012685:Sepharose
D003619:Dansyl Compounds; D003208:Concanavalin A; D012685:Sepharose; D005944:Glucosamine