Detection of labelled RNA species by contact hybridization.
An improved contact hybridization technique for the analysis of labelled RNA species is presented. The method combines high sensitivity of detection with the high resolution of polyacrylamide gel electrophoresis and should be especially useful for the characterization of transient RNA precursor molecules. Its application to gene mapping is illustrated.
D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones; D007668:Kidney / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D012617:Sea Urchins; D014981:Xenopus
D006657:Histones; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012313:RNA