[Filtration-fluorescence determination of DNA sedimentation profiles].
A method for determination of DNA sedimentation profiles in density gradients of sucrose or salts is proposed. The method consists in isolation and purification of DNA from the fractions by molecular filtration and a subsequent determination of DNA content by the fluorescence of the DNA-ethidium bromide complex.
D000818:Animals; D002499:Centrifugation, Density Gradient / Q000379:methods; D004247:DNA / Q000032:analysis*; D004996:Ethidium; D006801:Humans; D008970:Molecular Weight; D013050:Spectrometry, Fluorescence / Q000379:methods
D004247:DNA; D004996:Ethidium
Fil'tratsionno-fluorestsentnoe opredelenie profileĭ sedimentatsii DNK.