Composition and spectral properties of a cytochrome-enriched fraction obtained from chloroplasts by digitonin treatment.
A cytochrome-enriched fraction obtained from chloroplasts after treatment with the detergent digitonin contained cytochromes f, b-559LP and b-563 in the approximate proportions 1:1:2, close to those observed in unfractionated chloroplasts. The spectrum of cytochrome b-563 at temperature of liquid N2 showed a single asymmetrical alpha-band with a maximum at 561 nm.
D002736:Chloroplasts / Q000032:analysis* / Q000187:drug effects; D003580:Cytochromes / Q000032:analysis*; D004072:Digitonin / Q000494:pharmacology*; D013053:Spectrophotometry
D003580:Cytochromes; D004072:Digitonin