The effects of glucocorticoids on the distribution and mobilisation of arachidonic acid in fat cell ghosts.
1 The prostaglandin synthesizing enzymes were found to be present in fat cell ghosts isolated from rabbit adipose tissue. 2 Prostaglandin E2 (PGE2) and PGF2, were synthesized by ghosts after stimulation with adrenocorticotrophic hormone (ACTH). 3 Indomethacin was found to inhibit this synthesis but not the synthesis of lipoxygenase products. 4 When fat cell ghosts were stimulated by ACTH, fatty acid release was observed from both neutral lipids and phospholipids. 5 The arachidonic acid (AA) pool within the ghosts and identified: approximately 90% was present in the phospholipid fraction, 8.5% in the neutral lipids and 1.5% unbound. 6 The glucocorticoids were found to stimulate incorporation of [14C]-AA into neutral lipids and inhibit its incorporation into phospholipids. 7 When fatty acid release was stimulated with ACTH, the glucocorticoids were found to inhibit the mobilisation of [14C]-AA from the phospholipids and enhance its release from the neutral lipids. 8 The glucocorticoids inhibit prostaglandin formation in fat cell ghosts.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D066298:In Vitro Techniques; D008049:Lipase / Q000378:metabolism; D050356:Lipid Metabolism; D010743:Phospholipids / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism; D011817:Rabbits
D001095:Arachidonic Acids; D005227:Fatty Acids; D005938:Glucocorticoids; D010743:Phospholipids; D011453:Prostaglandins; D008049:Lipase