An evaluation of N-ethyl-maleimide-induced lipid peroxidation in platelet concentrates stored at 4 or 22 degrees C.
A method for the quality control of platelet viability, based on N-ethyl-maleimide (NEM)-induced lipid peroxidation, is reported. Duplicate platelet concentrates from individual donors were stored at 22 and 4 degrees C, respectively. At time 0 and at 24-hour intervals, pH, O2  and CO2 tensions, ADP-induced aggregation, glass adhesivity, hypotonic shock response (HSR) and NEM-induced lipid peroxidation were measured. A highly significant correlation between NEM-induced lipid peroxidation and HSR was established, which suggests that the former method can be used for the in vitro prediction of posttransfusion survival of preserved platelets.
D001792:Blood Platelets / Q000378:metabolism*; D003080:Cold Temperature; D005033:Ethylmaleimide / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans; D007038:Hypotonic Solutions; D050356:Lipid Metabolism; D008297:Male; D008315:Malondialdehyde / Q000096:biosynthesis; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D013696:Temperature
D007038:Hypotonic Solutions; D008315:Malondialdehyde; D005033:Ethylmaleimide