Human erythrocyte fraction in "Percoll" density gradients.
A new rapid method for the age fractionation of human erythrocytes by centrifugation through a "Percoll" density gradient is described. The fractionation is demonstrated by density-related changes in the volume, haemoglobin concentration, pyruvate kinase and acetylcholine esterase activities and potassium contents of the erythrocytes, and the distribution of reticulocytes on the gradients.
D000110:Acetylcholinesterase / Q000097:blood; D002469:Cell Separation; D002499:Centrifugation, Density Gradient / Q000379:methods*; D004905:Erythrocyte Aging; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000166:cytology*; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D011188:Potassium / Q000097:blood; D011770:Pyruvate Kinase / Q000097:blood; D012156:Reticulocytes / Q000166:cytology
D006454:Hemoglobins; D011770:Pyruvate Kinase; D000110:Acetylcholinesterase; D011188:Potassium