Single ionic channels observed in tissue-cultured muscle.
The extracellular patch clamp technique developed by Neher et al. to record the responses of single channels in skeletal muscle has provided firm evidence for the two-state nature of the conductance event in nicotinic endplate channels. We report here the use of the extracellular patch technique to record single-channel responses from tissue-cultured chick skeletal muscle cells. The temperature dependence of channel conductance and gating kinetics shows no evidence of discontinuous behaviour between 17 and 37 degrees C.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002794:Choline / Q000031:analogs & derivatives / Q000494:pharmacology; D004553:Electric Conductivity; D007473:Ion Channels / Q000502:physiology*; D007700:Kinetics; D009132:Muscles / Q000502:physiology*; D011950:Receptors, Cholinergic / Q000502:physiology*; D011978:Receptors, Nicotinic / Q000502:physiology*; D013696:Temperature
D007473:Ion Channels; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D002794:Choline