Different nucleosome structures on transcribing and nontranscribing ribosomal gene sequences.
Monomeric DNA lengths from Physarum nuclear chromatin occur in two subunit forms which differ from each other and from higher oligomers of nucleosomes in content of transcribed ribosomal DNA sequences. Labeled DNA restriction fragments from ribosomal RNA coding regions reanneal most rapidly with DNA from a monomeric subunit fraction. A particles, isolated from growing plasmodia and containing 144 base pairs of DNA in an extended conformation. Higher oligomers of nucleosomes are depleted in sequences from transcribing gene regions but are enriched in sequences from the nontranscribed central spacer of the ribosomal DNA palindrome. Nucleosome configuration on two 26S gene intervening sequences resembles that on adjacent coding regions.
D002466:Cell Nucleolus / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure; D005796:Genes; D008040:Genetic Linkage; D009707:Nucleosomes / Q000648:ultrastructure*; D010804:Physarum; D012335:RNA, Ribosomal / Q000235:genetics*; D014158:Transcription, Genetic
D002843:Chromatin; D009707:Nucleosomes; D012335:RNA, Ribosomal