A polyethylene glycol radioimmunoprecipitation assay for human immunoglobulin G.
A polyethylene glycol (PEG) radioimmunoprecipitation assay for human IgG is described that is sufficiently sensitive to detect 0.5 ng of IgG. This model antibody-antigen system was also used to study the stoichiometries of PEG-precipitated complexes. Our results suggest that the presence of PEG may affect the stoichiometry of the complexes which precipitate from solution.
D011232:Chemical Precipitation; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008832:Microchemistry; D011092:Polyethylene Glycols; D011863:Radioimmunoassay / Q000379:methods*
D007074:Immunoglobulin G; D011092:Polyethylene Glycols