title: Assessment of Dharmendra antigen. II. Standardisation of the antigens.
abstract: Dharmendra antigen with different bacterial counts (16, 12.5, 10, 7.5, 5 and 2.5 million/ml) have been utilized for determination of skin delayed hypersensitivity in leprosy patients. It has been noted that antigen with 10 million acid fast bacilli (AFB)/ml mounts a standard early (24 hrs) as well as late (3 weeks) reaction in patients. Lepromatous patients do not show any skin reaction with this dilution. Thus, a standard Dharmendra antigen has been prepared using a considerably smaller number of organisms as compared to the International standards for Mitsuda antigen.
mesh_terms: D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007916:Lepromin / Q000276:immunology; D007918:Leprosy / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012015:Reference Standards
chemical_list: D000942:Antigens, Bacterial; D007916:Lepromin