Binding of calcium by fragment 38-108 of pike parvalbumin.
Cyanogen bromide splitting of pike parvalbumin III was carried out and fragment 38-108 containing two calcium-binding domains was isolated. The obtained fragment binds calcium rather well (Kd = 1.6.10(-5) M), possesses a secondary structure, its CD spectrum changes after removal of calcium.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D005399:Fishes; D007700:Kinetics; D009124:Muscle Proteins; D010320:Parvalbumins; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding
D009124:Muscle Proteins; D010320:Parvalbumins; D010446:Peptide Fragments; D002118:Calcium