Selenium effects on the carcinogenicity and metabolism of 2-acetylaminofluorene.
Addition of 4 ppm Se to the drinking water of male albino rats fed diets containing 0.03% 2-acetylaminofluorene (AAF) provided protection against hepatic damage and also resulted in at least 50% reduction in liver tumor incidence. An in vitro assay system utilizing microsomes from Se supplemented or non-supplemented 3-methylcholanthrene (MC) induced rats was used to determine the effect of oral Se intake on the metabolism of AAF. Oral Se administration led to an increase in ring hydroxylation and a decrease in N-hydroxylation. Addition of Se to the microsomal assay system increased 3-OH AAF formation and decreased N-OH AAF formation, thus shifting the balance of metabolism toward detoxification pathways.
D015073:2-Acetylaminofluorene / Q000378:metabolism; D000818:Animals; D001711:Biotransformation / Q000187:drug effects; D006881:Hydroxyacetylaminofluorene / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D012643:Selenium / Q000494:pharmacology*
D006881:Hydroxyacetylaminofluorene; D008748:Methylcholanthrene; D015073:2-Acetylaminofluorene; D012643:Selenium