Long-term effect of glucagon administration on rat liver L-type pyruvate kinase.
After 5 h of treatment with glucagon, liver L-type pyruvate kinase (ATP: pyruvate 2-0-phosphotransferase; EC 2.7.1.40) showed a significant decrease of K0.5 and the Hill coefficient (nH) in the absence of fructose 1,6-diphosphate. However, in the presence of fructose 1,6-diphosphate, liver enzymes from treated rats showed a slight decrease of K0.5 but nH remained unchanged. In both circumstances, no changes of Vmax were observed after treatment. These changes in the kinetic properties of liver L-type pyruvate kinase are consistent with the dephosphorylation of the enzyme caused by insulin release in response to treatment with glucagon.
D000818:Animals; D005260:Female; D005635:Fructosediphosphates / Q000378:metabolism; D005934:Glucagon / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D011770:Pyruvate Kinase / Q000378:metabolism*; D051381:Rats
D005635:Fructosediphosphates; D005934:Glucagon; D011770:Pyruvate Kinase