Adenine cycle in hepatopancreocytes of Helix pomatia (Gastropoda).
Intact hepatopancreocytes were obtained from hibernating or active purinotelic snails, H. pomatia (Gastropoda). When incubated with [14C]glycine or [14C]formate, they synthesized de novo purine compounds, including also adenylates, adenosine and adenine. Hepatopancreocytes resynthesized also adenylates and other purine compounds from [3H]adenine or from [3H]adenosine split by the H. pomatia cell enzyme to adenine; the resynthesis of ADP+ATP was proportional to adenine concentration. Thus all reactions of the postulated adenine cycle: AMP leads to adenosine leads to adenine leads to AMP occur in the intact hepatopancreocytes; this cycle could probably be responsible for maintenance of the high level of adenylates during winter sleep.
D000225:Adenine / Q000096:biosynthesis*; D000241:Adenosine / Q000096:biosynthesis*; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D005561:Formates / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology; D006372:Helix, Snails / Q000166:cytology*; D006605:Hibernation; D008099:Liver / Q000166:cytology*; D010179:Pancreas / Q000166:cytology*; D011685:Purine Nucleotides / Q000096:biosynthesis*
D003470:Culture Media; D005561:Formates; D011685:Purine Nucleotides; D000225:Adenine; D000241:Adenosine; D005998:Glycine