[Change in the karyotypic structure of mouse and rat rhabdomyosarcomas on their transplantation into the anterior chamber of the eye].
A study has been made of 7 transplatable lines of mice rhabdomyosarcomas and one line of rat rhabdomyosarcoma during their transplantation into the eye anterior chamber subcutaneous tissue. In all, 10 subcutaneous transplants and 15 transplants into the eye anterior chamber (EAC) were examined. Etanol fixed print smears were subjected to the Feulgen reaction to measure the DNA content using a cytophotometer MCPhU-1; 100 cells being measured in each transplant. In the majority of the EAC transplants, a statistically significant decrease of the karyotypic variability was found in additionto the augmentation to the diploid cell ratio as compared to subcutaneously proliferating populations of the same tumour lines. In some cases EAC transplants displayed exclusively diploid (periploid) populations of tumour myoblasts. Shifts in the karyotypic structure of populations towards diploidy, revealed during the cultivation of transplantable rhabdomyosarcomas, may be regarded as a phenomenon of the "karyotypical normalization" of tumour cells. The disappearance or sharp decrease of tetraploid or hypertetraploid classes of cells in EAC transplants may be due to the increase of their selective value in condition of immunological privilege of diploid, karyotypically normal cells, and of reduction of the genome mutation frequency in a diploid fraction of tumor myoblast populations.
D000818:Animals; D000867:Anterior Chamber; D004273:DNA, Neoplasm / Q000235:genetics; D004171:Diploidy; D005134:Eye Neoplasms / Q000235:genetics*; D007621:Karyotyping; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000235:genetics; D051381:Rats; D012208:Rhabdomyosarcoma / Q000235:genetics*
D004273:DNA, Neoplasm
Izmenenie kariotipicheskoĭ struktury rabdomiosarkom mysheĭ i krys pri ikh transplantatsii v peredniuiu kameru glaza.