Cellular uptake of ribonucleic acids entrapped into liposomes.
Ribonucleic acids were entrapped into phospholipid vesicles (liposomes). After incubation of the liposomes containing RNA (L- RNA), the RNA was introduced into the cells. The kinetics of L- RNA uptake by the cells in culture were studied. The uptake of L- RNA is linear over a broad vesicle concentration range depending on temperature, and at 37 degrees C uptake levels reach a plateau after 3 hours. Inhibitors of cellular energy metabolism have little effect on the uptake, and thus fusion, as the main mechanism of uptake, is proposed.
D000085:Acetates / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D002460:Cell Line; D004705:Endocytosis; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D010743:Phospholipids; D012313:RNA / Q000378:metabolism*
D000085:Acetates; D001386:Azides; D008081:Liposomes; D010743:Phospholipids; D012313:RNA