Labelling of cardiolipin in vitro.
A method for labelling the polar head groups of cardiolipin is described. Labelling was carried out on sonicated cardiolipin/water suspensions. The free hydroxyl group of cardiolipin was oxidised with an excess of p-(diazonium) benzenesulfonic acid (DABS) and then reduced with NaB3H4. Isopropanol was oxidised in the presence of DABS to test the reactivity of the diazonium salts, and the reaction product was analysed by means of gas-chromatography. Labelled cardiolipin, identified by thin-layer chromatography (TLC), was chromatographically pure and identical to untreated cardiolipin. The hydrolysis of cardiolipin confirmed that the labelling was at the level of polar head groups.
D002308:Cardiolipins / Q000032:analysis*; D002855:Chromatography, Thin Layer; D007553:Isotope Labeling / Q000379:methods; D014316:Tritium
D002308:Cardiolipins; D014316:Tritium