Photoactivated covalent binding of [3H]bilirubin to human serum albumin.
A one-step procedure has been developed for the preparation of [3H]bilirubin IX-alpha in good yield from unlabelled bilirubin. Irradiation of an aqueous solution of [3H]bilirubin IX-alpha in the presence of human serum albumin results in the covalent attachment of the bilirubin to the protein. Preliminary degradation studies have been carried out to locate the site of attachment of the bilirubin to the albumin.
D001663:Bilirubin / Q000378:metabolism*; D001665:Binding Sites; D003488:Cyanogen Bromide / Q000494:pharmacology; D006801:Humans; D008027:Light; D011485:Protein Binding / Q000187:drug effects; D012709:Serum Albumin / Q000378:metabolism*; D014316:Tritium
D012709:Serum Albumin; D014316:Tritium; D003488:Cyanogen Bromide; D001663:Bilirubin