title: Decreased incorporation of L-[U-14C]serine into phosphatidylserine by polymorphonuclear leukocytes during phagocytosis.
abstract: A decreased rate of L-[U-14C]serine incoroporation into phosphatidylserine of polymorphonuclear leukocytes exposed to starch granules was observed. L-[U-14C]serine uptake was also depressed under identical conditions. The degree of reduction in specific radioactivity of phosphatidylserine was parallel to that of L-[U-14C]serine uptake. Both uptake and efflux of 45Ca2+ were enhanced in cells with starch granules, but no significant change in cellular calcium levels was detected. These results suggest that the reduced L-[U-14C]serine incorporation into phospholipids may be attributable to decreased availability of this amino acid. The involvement of Ca2+ fluxes in phosphatidylserine synthesis in intact leukocytes cannot, however, be excluded.
mesh_terms: D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D006168:Guinea Pigs; D009504:Neutrophils / Q000378:metabolism*; D010587:Phagocytosis; D010718:Phosphatidylserines / Q000096:biosynthesis*; D010743:Phospholipids / Q000096:biosynthesis; D012694:Serine / Q000378:metabolism*
chemical_list: D010718:Phosphatidylserines; D010743:Phospholipids; D012694:Serine; D002118:Calcium