Formation of nuclei during delay time prior to aggregation of deoxyhemoglobin S in concentrated phosphate buffer.
The delay time prior to aggregation of deoxyhemoglobin S was found to be shortened significantly if monomeric deoxyhemoglobin S was added near the end of the expected delay time. This suggests the formation of a preaggregation complex of deoxyhemoglobin S, probably so-called "nuclei", near the end of the delay time. In fact, the molecular weight of deoxyhemoglobin S as measured by a low angle laser light scattering photometer increased exponentially during the delay time prior to the visible aggregation of deoxyhemoglobin S. These results support the nucleation-controlled aggregation mechanism for the aggregation of deoxyhemoglobin S in concentrated phosphate buffer.
D001704:Biopolymers; D002021:Buffers; D006451:Hemoglobin, Sickle; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D010710:Phosphates; D013997:Time Factors
D001704:Biopolymers; D002021:Buffers; D006451:Hemoglobin, Sickle; D010710:Phosphates