Complement proteins and macrophages. 1. Quantitative estimation of factor B produced by mouse peritoneal macrophages.
Cobra venom factor was used for the detection of factor B synthesized by mouse peritoneal macrophages. This method was shown to be specific for factor B assay by neutralization by antimouse factor B antibody. The amount of factor B in the culture supernatant, assessed by this method, was found to be dependent on the medium used for cultivation of macrophages. The addition of 25% L cell-conditioned medium to minimal essential medium (LCM-MEM) enhanced the production of factor B and also of lysozyme. Kinetic analysis in LCM-MEM showed that factor B produced by 6 x 10(4) cells/cm2 increased up to 72 hr and reached a plateau at 96 hr. The amounts of factor B and lysozyme produced in LCM-MEM depended upon the number of macrophages. Production of factor B was completely inhibited by 1 microgram of cycloheximide per ml and was restored by its removal.
D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D011415:Complement Factor B / Q000096:biosynthesis* / Q000378:metabolism; D003165:Complement System Proteins; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004792:Enzyme Precursors / Q000096:biosynthesis*; D005643:Fucose / Q000494:pharmacology; D006461:Hemolysis; D007700:Kinetics; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice
D003470:Culture Media; D004792:Enzyme Precursors; D005643:Fucose; D003165:Complement System Proteins; D003513:Cycloheximide; D011415:Complement Factor B; D003078:Colchicine