Is antiglycolysis required for routine glucose analysis?
We obtained -68 pairs of simultaneously drawn serum and fluoride-oxalate plasma samples from patients and analyzed them by a continuous-flow (AutoAnalyzer II) glucose oxidase method. Glucose concentrations ranged from 370 to 3530 mg/L. Glucose concentrations for samples obtained in the fluoride-oxalate preservative averaged 42 +/- 35 mg/L (mean +/- SD) higher than serum. The magnitude of this difference was independent of glucose concentration. Linear-regression analysis of 270 pairs for which the time from collection to separation was recorded indicated that the difference between serum and plasma increased by 0.32 mg/L per minute of delay over a time span of 15 to 295 min. These differences are smaller than those described in standard textbooks. We conclude that, with the specimen-handling process used in our hospital, serum glucose determinations are clinically acceptable.
D001774:Blood Chemical Analysis / Q000592:standards; D001786:Blood Glucose / Q000032:analysis*; D001800:Blood Specimen Collection / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D005949:Glucose Oxidase; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D010070:Oxalates; D010949:Plasma / Q000032:analysis; D012969:Sodium Fluoride; D013997:Time Factors
D001786:Blood Glucose; D010070:Oxalates; D012969:Sodium Fluoride; D005949:Glucose Oxidase