Use of a semiselective medium to culture Legionella pneumophila from contaminated lung specimens.
Legionella pneumophila was successfully isolated, using a semiselective medium, from two of three lung specimens heavily contaminated with other organisms. This medium is composed of charcoal yeast extract agar, supplemented with vancomycin and polymyxin B. L. pneumophila was observed at 8 days on plates containing less than or equal to 40 units of polymyxin B and less than or equal to 1 microgram of vancomycin per ml.
D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D002606:Charcoal; D003470:Culture Media; D006801:Humans; D007877:Legionnaires' Disease / Q000382:microbiology*; D008168:Lung / Q000382:microbiology*; D011112:Polymyxin B; D013045:Species Specificity; D014640:Vancomycin
D003470:Culture Media; D002606:Charcoal; D014640:Vancomycin; D011112:Polymyxin B