Ovarian steroidogenesis in rats following inhibition of DNA synthesis after administration of mitomycin C.
Suppression of delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta-OHD) and glucose-6-phosphate dehydrogenase (G-6-PD) activities were observed in the rat ovarian tissues following treatment with Mitomycin C (MC), an antibiotic which depresses DNA synthesis. The same treatment also resulted in accumulation of cholesterol and ascorbic acid in the ovaries and a decrease of uterine weight. The atretic changes in the treated ovaries were judged from the activity of the lysosomal enzyme leucine amino-peptidase (LAP). The results suggest a diminution in ovarian steroid biogenesis following an alteration of DNA synthesis.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D003338:Corpus Luteum / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006651:Histocytochemistry; D008937:Mitomycins / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013256:Steroids / Q000096:biosynthesis*; D014599:Uterus / Q000187:drug effects / Q000502:physiology
D008937:Mitomycins; D013256:Steroids; D004247:DNA; D002784:Cholesterol; D015096:3-Hydroxysteroid Dehydrogenases; D005954:Glucosephosphate Dehydrogenase; D001205:Ascorbic Acid