A simple solid-phase radioimmunoassay for the measurement of IgG secreted in vitro by human lymphocytes.
A radioimmunoassay is described for measuring IgG, based on the ability of immunoglobulins of this class to inhibit the binding of radioiodinated staphylococcal protein A to IgG linked to a solid phase. The solid phase is represented by ox erythrocytes coated with anti-ox erythrocyte rabbit IgG, a reagent used for detecting cells equipped with receptors for the Fc fragment of IgG. By this assay the IgG secreted in vitro by human peripheral blood lymphocytes stimulated with PWM and those present in samples of very diluted human sera were measured. It was found that the assay is a very rapid, simple and reproducible procedure for the detection of IgG immunoglobulin at the nanogram level.
D000818:Animals; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002417:Cattle; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D011863:Radioimmunoassay; D011961:Receptors, Fc / Q000276:immunology; D013205:Staphylococcal Protein A / Q000276:immunology
D007074:Immunoglobulin G; D011961:Receptors, Fc; D013205:Staphylococcal Protein A