title: Transformation of primary rat kidney cells by DNA fragments of weakly oncogenic adenoviruses.
abstract: Primary cultures of baby rat kidney (BRK) cells were transformed by intact DNA and DNA fragments of weakly oncogenic human adenovirus types 3 and 7. The smallest fragment found to contain transforming activity was the left-terminal 4% endo R.HindIII fragment (for both adenovirus type 3 and 7 DNAs). The efficiency of transformation of this fragment was low, and no permanent cell line could be established. Left-terminal fragments ranging from 84 to 4,5% of the viral genome could all transform BRK cells with the same efficiency as intact viral DNA. A number of adenovirus type 7 DNA fragment-transformed lines were established and were found to contain persistent viral DNA sequences and adenovirus subgroup B-specific T antigen. Consequently, the transforming functions of adenovirus types 3 and 7 are located at the extreme left-hand end of the genome, and the minimum size for a DNA fragment with transforming activity is 1.0 X 10(6) daltons. These results do not rule out the possibility that viral genes located outside the transforming region may also influence transformation.
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics* / Q000502:physiology; D000818:Animals; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D007668:Kidney; D051381:Rats
chemical_list: D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D004279:DNA, Viral