An approach to the chemical quantitation of kinins for the assay of kininogenases.
A method was developed for the rapid isolation and identification of kallidin produced by hog pancreatic kallikrein on a partially purified bovine plasma kininogen preparation. By means of a reversed phase chromatography on a C18-silica gel column, kallidin was rapidly concentrated from the reaction mixture, and subsequently resolved on a thin-layer plate of silica gel 60. Fluorescamine was used to locate the kinin. Efforts to elute the kinin from the TLC plates for quantitation were not successful.
D000818:Animals; D002855:Chromatography, Thin Layer; D007610:Kallikreins / Q000032:analysis*; D007704:Kininogens; D007705:Kinins / Q000032:analysis*; D008722:Methods; D010179:Pancreas / Q000201:enzymology*; D013552:Swine
D007704:Kininogens; D007705:Kinins; D007610:Kallikreins