Structure of plant nuclear and ribosomal DNA containing chromatin.
Digestion of plant chromatin from Brassica pekinensis and Matthiola incana with staphylococcus nuclease leads to a DNA repeat of 175 plus or minus 8 and a core size of 140 base pairs. DNase I digestion results in multiples of 10 bases. Ribosomal RNN genes were studied as a model system for active plant chromatin because of their great redundancy and their high transcriptional activity in growing and differentiating tissues. The actively transcribed genes were identified by nascent RNA of ribosomal origin still attached to its matrix DNA. Hybridization techniques were used to demonstrate that even transcriptionally active gene sequences are present in nuclease generated chromatin subunits. Comparison of the DNase I kinetics of chromatin digestion with the amount of ribosomal RNA genes which is available for hybridization at the given times indicated that ribosomal RNA genes are digested, but not preferentially degraded by DNase I.
D001482:Base Composition; D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis* / Q000378:metabolism; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000302:isolation & purification; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000032:analysis* / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D012270:Ribosomes / Q000032:analysis* / Q000378:metabolism; D014158:Transcription, Genetic
D002843:Chromatin; D012313:RNA; D004247:DNA; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease