Affinity chromatography on concanavalin A-Sepharose of antigenic fractions of human seminal plasma.
Elution diagrams obtained on affinity chromatography show that the antigenic fractions of human seminal plasma studied, namely (a) the non-dialyzable components of human seminal plasma and (b) its trichloroacetic acid-soluble fraction, (c) the trichloroacetic acid-soluble fraction of whole human seminal plasma and (d) the pronase digested human seminal plasma, are complex mixtures of glycoproteins with minor amounts of polysaccharides. Some of these glycoproteins contain significant percentages of carbohydrates while others contain only trace amounts. Most of the glycoproteins carry non-reducing end-chain groups comprising alpha-D-glucopyranosyl, alpha-D-mannopyranosyl or sterically related residues.
D000941:Antigens / Q000032:analysis*; D002846:Chromatography, Affinity; D003208:Concanavalin A; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008297:Male; D011134:Polysaccharides / Q000032:analysis; D011402:Pronase; D012661:Semen / Q000032:analysis*; D012685:Sepharose; D014238:Trichloroacetic Acid
D000941:Antigens; D006023:Glycoproteins; D011134:Polysaccharides; D003208:Concanavalin A; D014238:Trichloroacetic Acid; D012685:Sepharose; D011402:Pronase