Human alveolar macrophages spontaneous reduction of BSPT salt.
Alveolar macrophages of non smoking and smoking human adults reduce BSPT salt spontaneously. The staining obtained is located on three cell membrane systems: the endoplasmic reticulum, the Golgi apparatus and the nuclear envelope. Methylene blue MB inhibits BSPT reduction. The smokers alvelolar macrophages have less positivity than those of the non smokers. The endogenous cell substrate revealed in this work is the initial common pathway of two different oxidative chains bounded to the microsome. One acts with cytochrome P 450 for chemical detoxification by hydroxylation, the other one acts with cytochrome B5 for lipid oxidation or peroxidation and both may be connected with the cell bactericidal system.
D052160:Benzothiazoles; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D006651:Histocytochemistry; D006801:Humans; D008264:Macrophages / Q000648:ultrastructure*; D009685:Nuclear Envelope / Q000648:ultrastructure; D010084:Oxidation-Reduction; D011650:Pulmonary Alveoli / Q000166:cytology; D012907:Smoking; D013778:Tetrazolium Salts
D052160:Benzothiazoles; D013778:Tetrazolium Salts; C024091:BSPT salt