[A method for the preparation of a Chlamydiae group-specific antigen on hela-229 cells infected with a strain of "Chlamydia trachomatis" for use in the complement fixation test (author's transl)].
A simple method for the preparation of a potent group-specific antigen on HeLa-229 cells infected with MRC-1 (LB) (TRIC/GB/MRC-1 Gf) strain of Chlamydia trachomatis is outlined. HeLa-229 cells are infected (MRC-1 strain, 102 inclusion-forming units per cell) with centrifugation at 4,000 g for 1 h in flat-bottomed vials. The cells are removed by brief trypsinization with 0.25% trypsin and put into 75 cm2 culture flasks (12 x 106 cells by flask) in BHK-21 medium supplemented with foetal bovine serum. The flasks are incubated for 5 days at 37 degrees C. The destroyed cell monolayer and the supernatant are centrifuged at 100,000 g for 1 h. The pellet is collected and resuspended in PBS and subjected to ultrasonic vibration for 30 min. This antigen may be used for detection of complement-fixing antibodies in lymphogranuloma venereum and ornithosis.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002689:Chlamydia / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000254:growth & development*; D003168:Complement Fixation Tests / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D008219:Lymphogranuloma Venereum / Q000175:diagnosis; D009956:Psittacosis / Q000175:diagnosis
D000942:Antigens, Bacterial
Préparation sur cellules HeLa-229 infectées par une souche de Chlamydia trachomatis d'un antigène de groupe des Chlamydiae fixant le complément.