Determination of the absolute configuration of mono-saccharides in complex carbohydrates by capillary G.L.C.
The absolute configuration of neutral monosaccharides, 2-acetamido-2-deoxy sugars, and uronic acids can be determined by capillary g.l.c. on SE-30 after glycosidation with (-)-2-butanol and protection of the remaining polar groups. The method is illustrated by application to mixtures of the constituent sugars of the capsular polysaccharide from Klebsiella type 57, alpha 1-acid glycoprotein, mucus glycoproteins, and the methylated, acidic polysaccharide from the coccoliths of Emiliania huxleyi (Lohmann) Kamptner.
D000818:Animals; D002236:Carbohydrate Conformation; D002849:Chromatography, Gas / Q000379:methods; D006023:Glycoproteins; D006801:Humans; D007709:Klebsiella / Q000032:analysis; D013058:Mass Spectrometry; D009093:Mucus / Q000032:analysis; D009961:Orosomucoid; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial
D006023:Glycoproteins; D009961:Orosomucoid; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial