[Growth and morphologic characteristics of normal human endometrial cells in primary culture].
Monolayer cultures of endometrium were established from 59 normal women after hysterectomy. The technical procedure is described, allowing cells in monolayer to grow satisfactorily for more than 60 days, showing a doubling-time of about 4-5 days. During early passages, two cell populations are characterized by microscopic examination : confluent areas of epithelial polygonal cells and scattered fusiform fibroblastic cells. After the 8th passage, epithelial cells are no longer recognized : the monolayer is homogeneous and all cells are fibroblast-like. These cells are an interesting model system for steroid sex hormone studies.
D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured / Q000166:cytology*; D004717:Endometrium / Q000166:cytology*; D004847:Epithelial Cells; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D009940:Organoids / Q000648:ultrastructure
Caractères de croissance et morphologie des cellules endométriales normales humaines en primo-culture.