Concanavalin A is mitogenic for resident peritoneal macrophages.
Con A, a known T-cell mitogen, is also mitogenic for resident peritoneal macrophages. The stimulated cells morphologically resemble macrophages and are actively phagocytic. The concentration of con A (30 micrograms/ml) required to stimulate 3H-TdR incorporation is ten times that required for T-cell activation. Con A must be present throughout the entire culture period to produce the maximum effect, and con A-depleted supernatant fluids from con A-stimulated cells cannot replace the con A requirement. Stimulation of 3H-TdR incorporation occurs after a 48-hour lag period and is maximal on the fifth to seventh day of culture. At the peak of the response, 20-30% of the macrophages can be stimulated to incorporate 3H-TdR, but little or no increase in the total number of cells present in the culture occurs. This and pulse-chase experiments indicate that only a single cycle of replication occurs in the stimulated cells. Con A-responsive peritoneal macrophages appear to be a distinct subpopulation and might play a different role in the interaction with T cells and B cells in the immune response than the con A-non-responsive cells.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D005260:Female; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008362:Mannosides / Q000494:pharmacology; D051379:Mice; D013268:Stimulation, Chemical; D013936:Thymidine / Q000378:metabolism
D008362:Mannosides; D003208:Concanavalin A; D004247:DNA; D013936:Thymidine