Effect of alpha-tocopherol on the binding of benzo(a)pyrene to nuclear macromolecules.
The binding of metabolically activated 14C-benzo(a)pyrene (BP) to rat liver nuclei was studied. The nuclear framework structure, termed the nuclear matrix, was found to bind BP preferably. alpha-Tocopherol inhibited the binding of BP to nuclear macromolecules in the presence of phenobarbital (PB)-induced microsomes. alpha-Tocopherol decreased the level of BP metabolites in nuclei, though it did not inhibit the activation of BP by PB-induced microsomes.
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D046911:Macromolecular Substances; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D001580:Benzopyrenes; D046911:Macromolecular Substances; D014810:Vitamin E; D008748:Methylcholanthrene; D010634:Phenobarbital