[Feed value of straw materials in the in vitro system. 4. Influence of the additional pre-incubation with the rumen fluid of animals deprived of food on the in vitro rate of protein synthesis].
Rumen fluid was extracted from sheep after they had been deprived of food for 48 hours. This rumen fluid was either directly applied in an artificial rumen or after pre-incubation (up to 16 hours). The 35S-incorporation rate into the microbial protein was measured after the admixture of untreated straw meal, straw meal treated with NH3-water of with NaOH. Generally, the 35S-incorporation rates were highest for the blank experiments. The reason for this is that the straw meal admixtures withdraw from the microorganisms low-molecular, energetically utilisable substrates by absorption and adsorption. Pre-incubation could not much reduce the relatively higher 35S-incorporation rate of the blank experiments. The two treated straw variants were significantly superior to the untreated straw meal concerning the 35S-incorporation rate.
D000821:Animal Feed; D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis*; D005215:Fasting; D009747:Nutritional Physiological Phenomena; D012417:Rumen / Q000382:microbiology*; D012756:Sheep
D001426:Bacterial Proteins
Untersuchungen zur Prüfung des Futterwertes von Strohmaterialien im in vitro-System. 4. Mitteilung. Einfluss einer zusätzlichen Vorinkubation von Pansensaft genüchterter Tiere auf die in vitro-Eiweisssyntheserate.