A mouse model of Pasteurella haemolytica infection and its use in assessment of the efficacy of P haemolytica vaccines.
A method for infecting mice with Pasteurella haemolytica is described. Mice were inoculated intraperitoneally with P haemolytica incorporated in gastric mucin, killed at various time intervals thereafter and viable counts of bacteria were performed on liver suspensions. P haemolytica grew at an exponential rate in the livers of normal mice. Mice vaccinated against P haemolytica A1 were protected against homologous challenge and viable counts decreased rapidly in their livers. Mice given trivalent P haemolytica vaccine (types A1, A2 and A6) were protected against challenge with P haemolytica types A1 and A6, but were not protected against challenge with types A2 and A9. These results correlate with findings in sheep.
D000818:Animals; D001428:Bacterial Vaccines; D004195:Disease Models, Animal; D005260:Female; D008099:Liver / Q000382:microbiology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010325:Pasteurella / Q000276:immunology* / Q000302:isolation & purification; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D012376:Rodent Diseases / Q000382:microbiology / Q000517:prevention & control*; D013154:Spleen / Q000382:microbiology; D014611:Vaccination / Q000662:veterinary
D001428:Bacterial Vaccines