[Morphologic changes in the ovary upon exposure to low temperatures (-196 degrees C) using cryoprotection].
The biological action of deep freezing (-196 degrees C) on the ovary with the purpose of establishing the possibility of using low temperatures for long-term storage of this tissue was studied. The use of polyethylene oxide of molecular weight 400 at an optimal concentration (20%) for freezing at a rate of 1 degree per minute to -8 degrees C, then 10 degrees/min to --196 degrees C permits to retain the functional activity of the ovary and restoration of its function after transplantation.
D000818:Animals; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003460:Crystallization; D005260:Female; D005615:Freezing; D006651:Histocytochemistry; D009926:Organ Preservation / Q000379:methods; D010053:Ovary / Q000033:anatomy & histology* / Q000187:drug effects / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism; D010477:Perfusion; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats
D003451:Cryoprotective Agents; D011092:Polyethylene Glycols; D010088:Oxidoreductases
Morfologicheskie izmeneniia v iaichnike v usloviiakh nizkikh temperatur (-196 gradusov C) s primeneniem krioprotektsii.