Simple and rapid method for the determination of progesterone in rat plasma by gas-liquid chromatography with electron-capture detection.
A method for estimating progesterone in rat plasma using gas-liquid chromatography (GLC) with electron-capture detection is described. By means of a column chromatographic technique for the extraction of progesterone from plasma, it is possible to isolate the steroid in such a specific way that, contrary to previously published methods, no additional chromatographic purification and separation steps are needed prior to the final GLC analysis. As a derivative for electron-capture detection we used the 3,20-di-O-pentafluorobenzyloxime of progesterone. This derivative is superior to those used hitherto with regard to sensitivity and stability. The high overall recovery (mean value, 89.4%) makes this method especially convenient for research work when only little plasma is available, or when the concentration of progesterone is very low.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D007202:Indicators and Reagents; D008832:Microchemistry; D011374:Progesterone / Q000097:blood*; D051381:Rats; D013739:Testosterone / Q000097:blood
D007202:Indicators and Reagents; D013739:Testosterone; D011374:Progesterone