Rapid purification of human erythrocyte 6-phosphogluconate dehydrogenase by means of affinity chromatography.
A rapid two-step procedure is reported by which homogeneous 6-Phosphogluconate dehydrogenase can be isolated from human erythrocytes. This method is based upon direct affinity chromatography of the hemolysates on adenosine 2',5'-bisphosphate-agarose (yielding a 4,500-fold purification), followed by anion exchange chromatography on a micro-column of DEAE-Sephadex. The present method represents a considerable simplification over previously available procedures for the purification of this enzyme protein from human erythrocytes.
D000244:Adenosine Diphosphate; D002021:Buffers; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D010734:Phosphogluconate Dehydrogenase / Q000097:blood*
D002021:Buffers; D000244:Adenosine Diphosphate; D010734:Phosphogluconate Dehydrogenase