Lipoprotein lipase of goat mammary tissue: comparison of extraction methods.
Mammary lipoprotein lipase of lactating goats was extracted by 3 methods: homogenization of tissue acetone-ether powders; aqueous homogenization of crude tissue using an Ultra-Turrax apparatus; aqueous homogenization of crude tissue using a Sorvall Omni-mixer microhomogenizer. Although there were differences between absolute values obtained by the 3 methods, each type of homogenate had a lipolytic activity with lipoprotein lipase characteristics (i.e. more than 90% inhibition by serum omission or NaCl addition). Furthermore, the 3 methods were highly correlated and presented similar variations with the stage of lactation, in parallel with long-chain fatty acid secretion into milk. Repeatability of the measure of homogenate lipolytic activities was about 8%, whereas day-to-day repeatability of enzyme extraction and assay was about 20% for each method.
D000818:Animals; D005260:Female; D006041:Goats / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000302:isolation & purification*; D008321:Mammary Glands, Animal / Q000201:enzymology*; D008722:Methods
D008071:Lipoprotein Lipase