[Thomson's colchicine test in differential diagnosis of lymphocytic leukemia and non-Hodgkin lymphoma].
The studies were performed to estimate the sensitivity of chronic lymphocytic leukaemia (CLL) lymphocytes (32 patients), non-Hodgkin lymphoma (NCL) lymphocytes (3 patients) and normal human (NH) lymphocytes (20 persons) to antimitotic concentration of colchicine in the conditions of the in vitro Thomson's test. Significant differences were observed in this sensitivity test: for CLL lymphocytes the mean values was 79%, for N-HL-lymphocytes 37%, for NH-lymphocytes--12%. These data show the possibility of practical use of Thomson's test in differential diagnosis of increased lymphocytosis. A quantitative heterogeneity of colchicine sensitive (pathological) and non-sensitive ("normal") CLL lymphocyte populations was observed among different CLL patients. There was no correlation between the proportion of the above populations and the patient's age, duration of illness and the magnitude of total lymphocytosis.
D000328:Adult; D000368:Aged; D002470:Cell Survival / Q000187:drug effects; D003078:Colchicine; D003937:Diagnosis, Differential; D006801:Humans; D066298:In Vitro Techniques; D007945:Leukemia, Lymphoid / Q000097:blood / Q000175:diagnosis*; D007958:Leukocyte Count; D008214:Lymphocytes / Q000187:drug effects; D008218:Lymphocytosis / Q000175:diagnosis*; D008223:Lymphoma / Q000097:blood / Q000175:diagnosis*; D008875:Middle Aged
D003078:Colchicine
Kolchicynowy test Thomsona w diagnostyce róznicowej białaczki limfocytowej przewlekłej.