[Preservation at -195 degrees C of trypsinized organs for primary cultures].
Trypsinized cells from rabbit kidney can be kept at -195, 8 degrees C indefinitely. When necessary, propagation in monolayer cultures is readily obtained as primary cultures, provided that some conditions be respected: 1) only glass culture flask must be used. Falcon plastic vessels do not allow cell attachment in this type of primary culture 2) the lag period is particularly long: it taken about 20 days before the beginning of cell multiplication 3) fetal calf serum is toxic for the cells in the 2nd and 3rd change of the culture medium, during the lag period.
D000818:Animals; D001793:Blood Preservation; D002478:Cells, Cultured; D046508:Culture Techniques; D005615:Freezing; D006801:Humans; D007668:Kidney; D009926:Organ Preservation; D011817:Rabbits; D014021:Tissue Preservation; D014357:Trypsin
D014357:Trypsin
Conservazione a -195,8 degrees C di organi tripsinizzati per colture primarie.