High-performance liquid chromatographic separation and detection of catecholamines and related compounds.
Four sets of chromatographic conditions are described for the separation and identification of selected catecholamines and related chemicals by high-performance liquid chromatography. Three mobile phases, three different columns and three detection systems, including ultraviolet absorption, fluorescence and electrochemical detection are reported. The use of detection response ratios as an additional means of identification is discussed and demonstrated. Nineteen compounds were studied and the retention times and detector responses are reported.
D002395:Catecholamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
D002395:Catecholamines