title: The detection of Y chromosomes in bloodstains--a reevaluation.
abstract: A method has been described for detecting Y chromosomes in the leukocytes of human bloodstains prepared on a variety of substrates. The factors that influence the proportion of chromosomes exhibiting a Y spot (the Y cell index) in a bloodstain are considered, including the subjective nature of assessment of the Y chromosome fluorescence, the substrate, and the age of bloodstain. In contrast to previous workers no decay in Y cell index with the age of the stain was observed. The results of a blind trial involving stains derived from case work, where from other evidence there was no doubt as to the sex of the donor, are presented. Sixty-five percent of the male bloodstains were correctly identified and no females were wrongly reported as male.
mesh_terms: D001801:Blood Stains; D002467:Cell Nucleus / Q000032:analysis; D004311:Double-Blind Method; D005260:Female; D005453:Fluorescence; D005898:Glass; D003368:Gossypium; D006801:Humans; D008297:Male; D009757:Nylons; D012730:Sex Chromosomes; D012732:Sex Determination Analysis / Q000379:methods; D013997:Time Factors; D014998:Y Chromosome
chemical_list: D009757:Nylons