[Primary mechanism of a reduction in the intensity of extremely weak mitochondrial luminescence in the process of chemical hepatic carcinogenesis].
The induced chemoluminescence in rat liver mitochondrial preparations was studied in the course of 2-acetylaminofluorene or N-nitrozodiethylamine induced hepatocarcinogenesis. As follows from the literature, the intensity of chemoluminescence is representative of catalase activity, i. e. of one of mitochondrial enzymes. Beginning from the stage of stimulation of pretumor cell proliferation, the course of carcinogenesis is caracterized by a progressive decrease in the intensity of chemoluminescence. An adrenomimetic noradrenaline induced a similar effect, whereas isoprenaline and alpha-adrenoblocator pirroxane stimulated chemoluminescence of mitochondria preparation in the intact rats. Ortobenzoquinone being oxidated with H2O2, noradrenaline and isoproteranol were deprived of oxidative activity. It is suggested that inhibition of mitochondrial catalase activity with endogeneous noradrenaline constitues a primary mechanism of the decrease in chemoluminescence intensity.
D015073:2-Acetylaminofluorene; D000818:Animals; D002374:Catalase / Q000378:metabolism; D004052:Diethylnitrosamine; D004789:Enzyme Activation / Q000187:drug effects; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000201:enzymology; D008163:Luminescent Measurements; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology; D009602:Nitrosamines; D051381:Rats
D009602:Nitrosamines; D004052:Diethylnitrosamine; D015073:2-Acetylaminofluorene; D002374:Catalase
O pervichnom mekhanizme snizheniia intensivnosti sverkhslabogo svecheniia mitokhondriĭ v protsesse khimicheskogo gepatokantserogeneza.