[Typing ACP isoenzymes in blood stains: test limits and temperature dependancy (author's transl)].
Two series of test blood stains of known red-cell acid phosphatase types A, AB, AC, B, BC, and C are applied to two different types of stain carriers (glass and cotton) and stored at different temperature ranges: (a) 37 degrees C at high air humidity, (b) 22 degrees C, (c) 4 degrees C, and (d) - 20 degrees C. Electrophoresis is carried out either on cellulose-acetate-foils or on thin agarose layers. Visualisation of the isoenzyme sites is achieved using umbelliferyl phosphate as a substrate. The most important results are: Electrophoresis on "Cellogel"-foils turns out advantageous compared to agarose since (a) less stain material is required, (b) reproducibility is rather reliable, and (c) electrophoretic separation is clearer. Blood stains on glass are identified after longer periods of storage than stains on cotton. If stains are stored at room temperature the time limits of demonstration are in the range of 3 to 6 weeks. The size of the sample necessary for demonstrating varies widely. Approximately twenty to twenty-five per cent of a blood drop are needed. The method described is recommended as practicable and reliable.
D000135:Acid Phosphatase / Q000097:blood*; D001801:Blood Stains; D004587:Electrophoresis, Agar Gel; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D010641:Phenotype; D013696:Temperature; D013997:Time Factors
D007527:Isoenzymes; D000135:Acid Phosphatase
Zur ACP-Typendifferenzierung aus Blutflecken: Nachweisgrenzen und Temperaturabhängigkeit.