Asparaginase production by human clinical isolates of Vibrio succinogenes.
Three human isolates of Vibrio succinogenes produced asparaginase. Apparent Km's were 87,220, and 320 microM. The rate of glutamine hydrolysis was between 2.8 and 3.5% of the rate of asparagine hydrolysis. Asparaginase production was not induced by ammonium ions, and enzyme yields were lower than those obtained with the rumen strain.
D001215:Asparaginase / Q000096:biosynthesis* / Q000378:metabolism; D001216:Asparagine / Q000378:metabolism; D002474:Cell-Free System; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D013045:Species Specificity; D013237:Stereoisomerism; D013379:Substrate Specificity; D014733:Vibrio / Q000201:enzymology*; D014735:Vibrio Infections / Q000382:microbiology*
D005973:Glutamine; D001216:Asparagine; D001215:Asparaginase