[14C-leucine incorporation by the nerve and glial cells of a cultured spinal ganglion].
Spinal ganglia of adult rabbits were cultured in the routine and protein synthesis precursors-enriched media. On days I and 4 of cultivation, the intensity of 14C-leucine incorporation in protein and in acid soluble fraction of nerve and glial cells was determined. The tissue of the spinal ganglion keeps incorporating 14C-amino acid, into neurons and glia, for all the tested periods of cultivation with both the media employed. The curves of incorporation into the above fractions of nerve and glial cells cultured in the routine medium display similar patterns of changes, whereas those obtained from the enriched medium observations appear to be anti-fasic. The enrichment of the medium results also in less pronounced fluctuations in the intensity of the labeled amino acid in protein and 14C-leucine pool, on the tested periods of cultivation, which may provide more stable conditions of the explant's survival.
D000818:Animals; D002250:Carbon Radioisotopes; D003470:Culture Media; D046508:Culture Techniques; D005727:Ganglia, Spinal / Q000166:cytology* / Q000378:metabolism; D007930:Leucine / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011817:Rabbits; D013997:Time Factors
D002250:Carbon Radioisotopes; D003470:Culture Media; D009419:Nerve Tissue Proteins; D007930:Leucine
Vkliuchenie 14C-leĭtsina nervnymi i glial'nymi kletkami kul'tiviruemogo spinal'nogo gangliia.