[Effects of 6-methyluracil and exogenous nucleic acids on DNA-synthesis of bone-marrow cells of rats following X-irradiation and tretamine or cyclophosphamide treatment (author's transl)].
The in vitro incorporation of 14C-thymidine into DNA of bone-marrow cells as well as DNA content of spleen and thymus of male Wistar rats were increased significantly by 6-methyluracil (within an N-methyl-D-glucosamine-6-methyluracil complex) and/or heterologous, low-molecular nucleic acids, when given therapeutically at doses of 100--250 mg/kg b.w. following i.v. injection of tretamine. Maximum effects were reached as early as two days after the onset of experiment. When tretamine was replaced by X-irradiation or cyclophosphamide, the influence of the 6-methyluracil complex and nucleic acids was less significant. Whereas normal bone-marrow cells and cells damaged in vitro by X-irradiation, tretamine and/or inhibitors of protein synthesis could not be influenced by the 6-methyluracil complex, incorporation of 14C-L-phenylalanine into proteins of bone-marrow cells was enhanced by exogenous DNA or RNA (10--100 micrograms/ml). The present investigations underline the previously drawn conclusions that certain biological alkylating agents, nucleic acids and pyrimidines may interfere to some extent through the reticuloendothelial system.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D003520:Cyclophosphamide / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D008297:Male; D009696:Nucleic Acids / Q000494:pharmacology*; D009929:Organ Size; D010649:Phenylalanine / Q000378:metabolism; D051381:Rats; D013154:Spleen / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects; D014265:Triethylenemelamine / Q000494:pharmacology*; D014498:Uracil / Q000031:analogs & derivatives* / Q000494:pharmacology; D014965:X-Rays
D009696:Nucleic Acids; D010649:Phenylalanine; D014498:Uracil; C008378:6-methyluracil; D003520:Cyclophosphamide; D004247:DNA; D014265:Triethylenemelamine; D013936:Thymidine
Wirkung von 6-Methyluracil und exogenen Nukleinsäuren auf die DNS-Synthese von Knochenmarkszellen der Ratte nach Einwirkung von Röntgenstrahlen, Tretamin oder Cyclophosphamid.