title: Disassembly of tobacco mosaic virus by membrane lipid isolated from tobacco leaves and polyornithine.
abstract: In vitro disassembly of tobacco mosaic virus (TMV) virions occurred in the presence of both polyornithine and a lipid fraction isolated from tobacco leaf membrane. The latter could be replaced by lecithine. Disassembly of 10 microgram of TMV virions was attained in the presence of a 500-mg leaf equivalent of membrane lipid and 20 microgram of polyornithine in 1 ml of 0.01 M Tris-HCl buffer, pH 7.4 at 30 C. Similarity and dissimilarity between the in vitro disassembly and the in vivo uncoating  mechanisms are discussed.
mesh_terms: D008563:Membrane Lipids / Q000502:physiology*; D009952:Ornithine / Q000031:analogs & derivatives / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D010947:Plants, Toxic; D012367:RNA, Viral / Q000378:metabolism; D012260:Ribonucleases / Q000494:pharmacology; D014026:Nicotiana; D014027:Tobacco Mosaic Virus / Q000187:drug effects / Q000502:physiology*; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D008563:Membrane Lipids; D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins; C008973:polyornithine; D009952:Ornithine; D012260:Ribonucleases