Ultrastructural characteristics of dog and human platelets adherent to native and collagen-coupled cuprophan.
Adhesion of human and dog platelets to native and collagen-coupled Cuprophan under defined flow conditions was studied by scanning and transmission electron microscopy. Dog platelets, singly adherent to and uniformly distributed on both native and collagen-coupled Cuprophan, extend slender pseudopods across the surface without evidence of degranulation. Human platelets, while not adhering to native Cuprophan, formed irregularly shaped, semi-confluent cytoplasmic sheets on the collagen-coupled surface. Extensive cytoplasmic reorganization and degranulation suggests a post-release state of the human platelets. Aspirin had no apparent effect on either human or dog platelet adhesion or upon the apparent release state of the human platelets.
D000327:Adsorption; D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure*; D002482:Cellulose / Q000031:analogs & derivatives*; D003094:Collagen; D003300:Copper; D003593:Cytoplasm / Q000648:ultrastructure; D004285:Dogs; D006801:Humans; D008567:Membranes, Artificial; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011554:Pseudopodia / Q000648:ultrastructure; D013499:Surface Properties
D008567:Membranes, Artificial; D003300:Copper; D002482:Cellulose; D003094:Collagen; C009865:cuprammonium cellulose; D001241:Aspirin