title: Tumor metastases and cell-mediated immunity in a model system in DBA/2 mice. VIII. Expression and shedding of Fc gamma receptors on metastatic tumor cell variants.
abstract: The expression of receptors for the Fc portion of IgG immunoglobin molecules was studied on tumor cell lines with high and low metastatic capacity. Two tumor cell lines from DBA/2 mice that had high metastatic activity, ESb and MDAY-D2, contained a high percentage of Fc receptor positive cells, as detected in a rosette assay with IgG antibody-coated erythrocytes (EA). In contrast, the low metastatic parental line Eb, from which ESb was derived, contained only a low percentage of EA-rosette-forming cells. ESb ascites tumor cells adapted to tissue culture in the presence of 2-mercaptoethanol (2ME) had a high expression of Fc receptors, whereas a cell line adapted to tissue culture in the absence of 2ME had a low expression of Fc receptors. "Soluble" Fc receptors were detectable by their ability to bind to EA and to cause blocking of rosette formation. They were found to be present in fluids from tumor-bearing animals, such as serum and cell-free ascites. Even animals with an ascites tumor of the low-metastatic line Eb contained "soluble" Fc receptors. The results are discussed with regard to their possible significance for tumor metastasis.
mesh_terms: D000818:Animals; D002460:Cell Line; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D008811:Mice, Inbred DBA; D009362:Neoplasm Metastasis / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology; D011961:Receptors, Fc / Q000378:metabolism*; D012397:Rosette Formation; D013154:Spleen / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D011961:Receptors, Fc