In vivo distribution of Tc-99m(Sn)pyridoxylideneisoleucine: effects of storage and labeling conditions. Concise communication.
Various conditions for the storage of the stannous pyridoxylideneisoleucine (Sn-P.isoL) kit reagent, and also for the preparation of Tc-99m(Sn)P. isoL, have been evaluated experimentally using rats. Both the frozen (-30 degrees C) and the freeze-dried state were found to be suitable for storage of the kit reagent. For the labeling procedure, at least 40 min incubation time (Sn-P.isoL + 99mTcO4-) was necessary at room temperature, but the same superior results could be obtained quickly by heating the mixed solution for 2 min in a boiling-water bath. The pertechnetate could be added in a volume up to four times that of the kit reagent. MoVI) and/or AI(III) in the pertechnetate solution did not interfere with the formation of Tc-99m(Sn)P.isoL up to the highest tested concentration of 40 micrograms/ml each.
D000818:Animals; D004356:Drug Storage; D007532:Isoleucine / Q000031:analogs & derivatives* / Q000378:metabolism; D007553:Isotope Labeling; D015609:Organotechnetium Compounds; D011730:Pyridoxal / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D011933:Reagent Kits, Diagnostic; D013667:Technetium / Q000378:metabolism*; D014018:Tissue Distribution
D015609:Organotechnetium Compounds; D011933:Reagent Kits, Diagnostic; C023593:technetium Tc 99m (Sn)pyridoxylideneisoleucine; D007532:Isoleucine; D011730:Pyridoxal; D013667:Technetium