title: Use of sodium salicylate as a blocking agent for cortisol-binding-globulin in a radioimmunoassay for cortisol on unextracted plasma.
abstract: This report describes investigations into the use of sodium salicylate as a cortisol-binding-globulin blocking agent and the subsequent development of a radioimmunoassay for cortisol on unextracted plasma. Cortisol antiserum was raised against a cortisol 3-O-(carboxy-methyl) oxime-bovine serum albumin conjugate. A 125I-labelled cortisol-tyrosine methyl ester conjugate was also prepared for use in the assay. The radioimmunoassay developed involved no pre-treatment of extraction of the samples before analysis and was extremely simple to perform. Comparison with another radioimmunoassay for cortisol and with the Mattingly fluorimetric assay gave good correlation.
mesh_terms: D000817:Anilino Naphthalenesulfonates; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods*; D012712:Serum Globulins / Q000378:metabolism*; D012980:Sodium Salicylate; D013849:Thimerosal
chemical_list: D000817:Anilino Naphthalenesulfonates; D012712:Serum Globulins; D013849:Thimerosal; D006854:Hydrocortisone; D012980:Sodium Salicylate