Characterization of a Friend virus-replicating target cell.
The FV-replicating target cell characterized in this study is an erythroid precursor cell, as shown by its response to bleeding and hypertransfusion. The target cell is not identical with the BFU-E compartment as demonstrated by different model velocities and different cell cycle characteristics as compared to BFU-E. Different sensitivity of FV-target cells and CFU-E to AMD, differences in the growth kinetics of both cell populations in bone marrow and spleen of mice after bleeding, and large quantitative differences of both cell populations in hemopoietic organs, suggest only partial identity or non-identity  of these two cell types. Finally it is shown that spleen colonies originating from FV-infected target cells are a primary erythroleukemic lesion, since they contain more Ep-independent CFU-E than do intercolony areas of the spleen.
D000818:Animals; D001803:Blood Transfusion; D001854:Bone Marrow Cells; D002472:Cell Transformation, Viral / Q000187:drug effects; D003114:Colony-Forming Units Assay; D003609:Dactinomycin / Q000494:pharmacology; D004920:Erythropoiesis / Q000187:drug effects; D005622:Friend murine leukemia virus; D006470:Hemorrhage; D006918:Hydroxyurea / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000209:etiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology
D003609:Dactinomycin; D006918:Hydroxyurea