Gas-liquid chromatographics determination of Bayer 73 in fish, aquatic invertebrates, mud, and water.
A gas-liquid chromatographic (GLC) method is described for determining residues of Bayer 73 (2-aminoethanol salt of 2',5-dichloro-4'-nitrosalicylanilide) in fish muscle, aquatic invertebrates, mud, and water by analyzing for 2-chloro-4-nitroaniline (CNA), a hydrolysis product of Bayer 73. Bayer 73 residues are extracted from fish muscle tissue, invertebrates, and mud with acetone-formic acid (98+2), and partitioned from water samples with chloroform. After sample cleanup by solvent and acid-base partitioning, the concentrated extract is hydrolyzed with 2N NaOH and H2O2 for 10 min at 95 degrees C. The CNA is then partitioned into hexane-ethyl ether (7+3) and determined by electron capture GLC. Average recoveries were 88% for fish, 82% for invertebrates, 82% for mud, and 98% for water at 3 or more fortification levels.
D000818:Animals; D002849:Chromatography, Gas; D004983:Ethanolamines / Q000032:analysis; D005399:Fishes / Q000378:metabolism*; D007448:Invertebrates / Q000032:analysis*; D009534:Niclosamide / Q000031:analogs & derivatives* / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D012989:Soil Pollutants / Q000032:analysis*; D012997:Solvents; D014873:Water Pollutants / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D004983:Ethanolamines; D010573:Pesticide Residues; D012989:Soil Pollutants; D012997:Solvents; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D009534:Niclosamide