Simultaneous determination of biliary cile acids in rat: electron impact and ammonia chemical ionization mass spectrometric analyses of bile acids.
Bile acids in the rat bile were fractionated into unconjugated, glycine- and taurine-conjugated fractions by employing piperidino-hydroxypropyl Sephadex LH-20 ion-exchange chromatography. Subsequently, these fractions were analyzed by gas-liquid chromatography (GLC) and GLC-mass spectrometry using a Silicone AN-600 column. Not only lithocholic acid, deoxycholic acid, chenodeoxycholic acid, hyodeoxycholic acid, ursodeoxycholic acid and cholic acid, but also alpha- and beta-muricholic acids were quantitatively and simultaneously detectable in conjugated and unconjugated fractions, respectively. In the unconjugated and conjugated fractions, varying amounts of the unidentified bile acid were detected upon GLC. The electron impact and ammonia chemical ionization mass spectrometric results and catalytic hydrogenation on the compound indicate that this bile acid seems to be a derivative of beta-muricholic acid having a double bond in the side chain. The present method is suitable to the simultaneous and quantitative determination of unconjugated and glycine- and taurine-conjugated bile acids in the rat bile.
D000818:Animals; D001646:Bile / Q000032:analysis*; D001647:Bile Acids and Salts / Q000032:analysis*; D002635:Chenodeoxycholic Acid / Q000032:analysis; D002793:Cholic Acids / Q000032:analysis; D003840:Deoxycholic Acid / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008095:Lithocholic Acid / Q000032:analysis; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D051381:Rats; D014580:Ursodeoxycholic Acid / Q000032:analysis
D001647:Bile Acids and Salts; D002793:Cholic Acids; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; C004821:muricholic acid; D008095:Lithocholic Acid; D014580:Ursodeoxycholic Acid