Ox kidney uricase: a new method of purification.
A purification procedure of uricase from ox kidneys has been worked out starting from a n-butanol extract. Gel filtration and ion-exchange chromatography give a highly purified enzyme, with a specific activity of 50, which corresponds to a 3,800 fold purification, and is almost homogeneous at the gel electrophoresis.
D000818:Animals; D002417:Cattle; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D007668:Kidney / Q000201:enzymology*; D013552:Swine; D014503:Urate Oxidase / Q000302:isolation & purification / Q000378:metabolism*
D014503:Urate Oxidase