Attenuated live fowl cholera vaccine. I. Development of vaccine strain M3G of Pasteurella multocida.
A live cholera vaccine was developed from a virulent avian septicemia strain of Pasteurella multocida serotype 1. The virulent parental strain was mutagenized with N-methyl-N'-nitro-N-nitroso guanidine. Mutants were selected that had either smaller colonies at 37 C or temperature sensitivity for growth at 41 C. Four small-colony mutants and 2 temperature-sensitive mutants were studied. All the mutants were avirulent for turkeys. Sixteen days after turkeys were vaccinated with each mutant, both the vaccinates and unvaccinated controls were challenge-exposed to virulent P. multocida of the homologous serotype and the heterologous serotype 3. Two of the small-colony mutant strains protected against both homologous and heterologous challenge. Suggested for a live cholera vaccine is P. multocida M3G, a small-colony-forming mutant, innocuous for both mice and turkeys and stable against reversion.
D000818:Animals; D001428:Bacterial Vaccines; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009154:Mutation; D010325:Pasteurella / Q000187:drug effects / Q000254:growth & development* / Q000276:immunology; D010326:Pasteurella Infections / Q000517:prevention & control / Q000662:veterinary; D011201:Poultry Diseases / Q000517:prevention & control; D013696:Temperature; D014422:Turkeys; D014613:Vaccines, Attenuated
D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated; D008769:Methylnitronitrosoguanidine