Characterization of the human peripheral effector cells mediating antibody dependent cellular cytotoxicity against allogenic cells.
The effector cell populations in human peripheral blood responsible for antibody-dependent cellular cytotoxicity against allogenic cells coated with HLA polyspecific antibodies were investigated using several separation techniques including preparative electrophoresis. Electrophoresis produced a marked effector cells enrichment in a range of 2--5 fractions which exhibited an intermediary electrophoretic mobility. Monocytic cells do not contribute an effector mechanism but minor subsets of polymorphonuclear cells and nylon wool non-adherent non-phagocytic lymphocytes displayed ADCC. Both effector cell populations were found to exhibit a similar electrical charge of cell surface centered around -1.05 micrometer . sec-1 V-1 cm. These observations provided a precise biophysical basis for the identification of effector cells in ADCC.
D000328:Adult; D000920:Antibody-Dependent Cell Cytotoxicity; D002469:Cell Separation; D004586:Electrophoresis; D004950:Esterases / Q000378:metabolism; D006801:Humans; D007694:Killer Cells, Natural / Q000201:enzymology / Q000276:immunology*; D008564:Membrane Potentials; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology
D004950:Esterases