Methylation analysis in glycoprotein chemistry. General procedure for quantification of the products of solvolysis of permethylated glycopeptides and glycoproteins.
A technique for the gas chromatographic analysis of the products of solvolysis of permethylated glycopeptides and glycoproteins has been developed. It involves methanolysis of a permethylated compound, quantitative transformation of methyl ethers of methyl glycosides into the corresponding O-trimethylsilyl(TMS)-O-methylalditols [2-deoxy-2-(N-methyl)acetamido-O-TMS-O-methylalditols in the case of hexosamine derivatives] and gas chromatographic quantification using a single column packed with 0.4% OV-225 on surface-modified Chromosorb.
D000117:Acetylglucosamine / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods; D005690:Galactose / Q000032:analysis; D006020:Glycopeptides; D006023:Glycoproteins; D007202:Indicators and Reagents; D008358:Mannose / Q000032:analysis; D008745:Methylation; D010047:Ovalbumin
D006020:Glycopeptides; D006023:Glycoproteins; D007202:Indicators and Reagents; D010047:Ovalbumin; D008358:Mannose; D000117:Acetylglucosamine; D005690:Galactose