Studies on the biotransformation of paeonol by means of isotope tracer techniques.--Synthesis and physicochemical properties of carbon-13 and deuterium labeled compounds--.
In order to perform the metabolic study of paeonol (I) in human and animals by using isotope tracer techniques, synthesis of I and its urinary metabolites labeled with carbon-13 or deuterium was investigated. 2-Hydroxy-4-methoxy[d3]acetophenone, 2,5-dihydroxy-4-methoxy[d3]acetophenone, and resacetophenone[acetyl-13C2] were synthesized from methyl iodide-d3, dimethyl sulfate-d6, and acetic acid-1,2-13C2, respectively. Physicochemical studies by IR and NMR spectra showed that stable isotope was not eliminated during the synthetic process of each compound. For the quantification of the metabolites by GC-MS analysis, isotope effect on gas chromatography and fragmentation was studied.
D000098:Acetophenones / Q000032:analysis* / Q000378:metabolism; D002250:Carbon Radioisotopes; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D013055:Spectrophotometry, Infrared
D000098:Acetophenones; D002250:Carbon Radioisotopes; C013638:paeonol; D003903:Deuterium