Separation of polypeptide chains of ricin and the interaction of the A chain with Cibacron Blue F3GA.
From ricin bound to the galactomannan guar gum in a column, the nonbinding toxic A chain could be eluted by reduction with 2-mercaptoethanol and later the B chain by lactose. The presence of a nucleotide-binding domain on the toxic chain A could be demonstrated from its interaction with the blue dye Cibacron Blue F3GA.
D000873:Anthracenes; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D004396:Coloring Agents; D009711:Nucleotides; D010446:Peptide Fragments / Q000302:isolation & purification; D012276:Ricin; D014227:Triazines
D000873:Anthracenes; D004396:Coloring Agents; D009711:Nucleotides; D010446:Peptide Fragments; D014227:Triazines; C012842:Cibacron Blue F 3GA; D012276:Ricin