[Characteristic of sorption properties of skeletal muscle nuclei in the normal state and with E-avitaminotic muscular dystrophy].
Sorption properties of skeletal muscles nuclei in rabbits in normal state and with E-avitaminosis were studied using organic dyes: neutral red (cationic) and turquoise direct light-fast "K" (anionic) and the influence of calcium-modified membrane of nuclei on their sorption. The nuclear surface is established to have both positive and negative charged groups sorbing turquioise direct and neutral red, respectively. The maximum volume of the dyes binding and the dissociation constants of the membrane-dye complex are estimated. It is shown that with muscular dystrophy the number of charged groups of both signs on the nuclear surface decreases. Calcium ions decrease the cationic dye sorption both in the normal state and with dystrophy and insignificantly decrease the anionic dye with dystrophy.
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism; D006651:Histocytochemistry; D007700:Kinetics; D009137:Muscular Dystrophy, Animal / Q000209:etiology / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism; D011817:Rabbits; D014811:Vitamin E Deficiency / Q000150:complications / Q000378:metabolism*
D004396:Coloring Agents; D002118:Calcium
Kharakteristika sorbtsionnykh svoĭstv iader skeletnykh myshts v norme i pri E-avitaminoznoĭ myshechnoi distrofii.