A simple procedure for the determination of carbamazepine in plasma by high pressure-liquid chromatography.
We describe a simple, rapid procedure for the estimation of carbamazepine in plasma. Protein is precipitated, and extraction is achieved by the addition of acetonitrile containing the internal standard N-acetyltryptophan ethyl ester. Separation is by reverse-phase high-pressure liquid chromatography with an acetonitrile: water mobile phase, and detection is by UV absorption at 280 nm. Total retention time is less than 7 minutes. Initial results gave within-batch and between-batch coefficients of variation of less than 2%, and mean recovery of 97%. The method is free from interference by other common anticonvulsant drugs.
D002220:Carbamazepine / Q000097:blood* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004827:Epilepsy / Q000097:blood / Q000188:drug therapy; D006801:Humans
D002220:Carbamazepine