Detection of endogenous salsolinol in neonatal rat tissue by a radioenzymatic--thin-layer chromatographic assay.
A sensitive radioenzymaticc--thin-layer chromatographic assay for the quantitative analysis of the tetrahydroisoquinoline alkaloid, salsolinol, in plasma and neonatal rat tissue is described. The assay involves the enzymatic O-methylation of salsolinol by catechol-O-methyltransferase in presence of [3H]S-adenosylmethionine, and subsequent separation by thin-layer chromatography of the resultant [3H]O-methyl-salsolinol from the O-methylated derivatives of dopamine, epinephrine and norepinephrine. The method allows the detection of as little as 100 pg salsolinol per g tissue, and the accurate quantitation of as little as 100 pg/ml plasma and 500 pg/g tissue. This assay permitted the detection of trace amounts of endogenous salsolinol in neonatal rat tissue (less than 500 pg/g tissue).
D000818:Animals; D000831:Animals, Newborn; D002394:Catechol O-Methyltransferase; D002855:Chromatography, Thin Layer / Q000379:methods*; D007546:Isoquinolines / Q000097:blood*; D051381:Rats; D012490:Salsoline Alkaloids / Q000097:blood*; D014316:Tritium
D007546:Isoquinolines; D012490:Salsoline Alkaloids; D014316:Tritium; D002394:Catechol O-Methyltransferase