title: Volatile hydrocarbon and carbonyl products of lipid peroxidation: a comparison of pentane, ethane, hexanal, and acetone as in vivo indices.
abstract: A study was undertaken to determine whether respiratory hexanal and acetone as well as pentane and ethane could be measured as potential indices of lipid peroxidation in vivo. The tests of induction of lipid peroxidation in rats included injection of iron-dextran and the vitamin E deficiency status. Injection of 460 mg of iron/100 g body wt over a 28-day period increased pentane and ethane production 4- and 6-fold, respectively. Hexanal production was increased 7-fold after injection of 60 mg of iron/100 g body wt, and then it fell back to the preinjection level in spite of continued injection of iron-dextran. Acetone production was lower in iron-injected rats than in controls, and it was ca. 10-fold higher in fasted vitamin E-deficient rats than in vitamin E-supplemented rats, being ca 48 and 5 nmol/100 g/min, respectively. It was observed that halomethane injection did not increase hexanal production, while acetone and pentane production were increased. Pentane and hexanal, but not acetone, were found to arise from decomposition of linoleic acid hydroperoxide in vitro. It was concluded that hydrocarbon gases are better indices of lipid peroxidation than hexanal, which is enzymatically metabolized, and acetone, the production of which is dominated by factors such as altered carbohydrate metabolism.
mesh_terms: D000096:Acetone / Q000032:analysis*; D000818:Animals; D003911:Dextrans / Q000494:pharmacology; D006838:Hydrocarbons / Q000032:analysis*; D007501:Iron / Q000494:pharmacology; D008054:Lipid Peroxides / Q000032:analysis*; D008297:Male; D051381:Rats; D012119:Respiration; D014811:Vitamin E Deficiency / Q000378:metabolism
chemical_list: D003911:Dextrans; D006838:Hydrocarbons; D008054:Lipid Peroxides; D000096:Acetone; D007501:Iron