The effect of time of death on extravascular tissue/blood secobarbital concentration ratios in the rat.
Extravascular liver/blood and brain/blood ratios were found to be an average of 6% and 1% higher, respectively, in all experiments than total liver/blood and brain/blood ratios. This difference may be informative in establishing true tissue levels. There was a significant time effect (P less than 0.05) with the extravascular liver/blood ratios but not with the extravscular brain/blood ratios. Extravascular liver/blood ratios were slightly higher in phenobarbital-pretreated animals than in non-pretreated animals. Tissue secobarbital levels in pretreated and non-pretreated animals are not different at 1/4 or 1 h, even though pretreated animals received higher doses than non-pretreated animals. Tissue levels are significantly higher (P less than 0.01) in pretreated animals than in non-pretreated animals at 4 h. It is possible that, at this time period, the barbiturate-metabolizing enzymes have become saturated or exhausted.
D000818:Animals; D001810:Blood Volume / Q000187:drug effects; D001921:Brain / Q000098:blood supply / Q000378:metabolism; D001923:Brain Chemistry; D003643:Death; D007928:Lethal Dose 50; D008099:Liver / Q000032:analysis* / Q000098:blood supply; D008297:Male; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D012631:Secobarbital / Q000008:administration & dosage / Q000032:analysis / Q000097:blood* / Q000494:pharmacology; D013997:Time Factors
D012631:Secobarbital; D010634:Phenobarbital