[A fast and simple micromethod for estimating proteins].
The assay is based on the property of proteins to form a complex with bromophenol blue. In alkaline solution this complex shows an intensive blue colour which is well measurable at 598 nm. The method is especially useful for small volumes (5-50 microliter) with a content of 5-30 micrograms of protein. By modification the test can be also used for the measurement of higher amounts of protein.
D001978:Bromphenol Blue; D003124:Colorimetry / Q000379:methods; D007202:Indicators and Reagents; D008832:Microchemistry; D011506:Proteins / Q000032:analysis*
D007202:Indicators and Reagents; D011506:Proteins; D001978:Bromphenol Blue
Eine einfache und schnelle Mikromethode zur Proteinbestimmung.