Factors influencing the assessment of anticoccidial activity in cell culture.
A comparative study has been made of the factors influencing the assessment of anticoccidial potency in vitro against Eimeria tenella using established anticoccidials and arprinocid and some of its analogues. Drugs whose potency depended upon medium composition were amprolium, lasalocid and halofuginone. There was a difference in strain sensitivity with robenidine. Host cell type had an important effect on potency of monensin, decoquinate, arprinocid and its analogues. Arprinocid was active in chick liver cell systems but totally inactive in chick kidney cell systems, although its N-oxide was active in both cell types. Arprinocid-containing medium, conditioned by supporting the growth of chick embryo liver cell cultures, had an anticoccidial effect on E. tenella growing in chick kidney cells. It is deduced that the anticoccidial activity of arprinocid in the chick is due to a metabolite.
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001593:Benzyl Compounds / Q000494:pharmacology; D002642:Chick Embryo; D002645:Chickens; D003049:Coccidiostats / Q000378:metabolism / Q000494:pharmacology*; D003470:Culture Media; D046508:Culture Techniques; D004539:Eimeria / Q000187:drug effects*; D007668:Kidney; D008099:Liver
D001593:Benzyl Compounds; D003049:Coccidiostats; D003470:Culture Media; C016245:arprinocid; D000225:Adenine