RNA-primed DNA synthesis by an enzyme preparation of calf thymus containing highly enriched RNA polymerase B and DNA polymerase.
RNA polymerase B and DNA polymerase alpha were highly enriched simultaneously from calf thymus. It was shown that the preparation exhibits RNA-synthesizing activity, which is able to stimulate in vitro DNA synthesis by DNA polymerase alpha by its preceding RNA synthesis. A part of the DNA was found to be covalently attached to RNA in Cs2SO4 equilibrium gradients after denaturation by formamide.
D000818:Animals; D002417:Cattle; D004257:DNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D012319:RNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D013950:Thymus Gland / Q000201:enzymology*; D014158:Transcription, Genetic
D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase