Paired-ion reversed-phase high-performance liquid chromatography of phenol sulfates in synthetic mixtures, algal extracts and urine.
Phenol sulfate esters have been analyzed by paired-ion reversed-phase high-performance liquid chromatography. The method provided direct, rapid chromatography of phenol sulfates in crude extracts of the red alga (Polysiphonia lanosa (2,3-dibromo-4,5-dihydroxybenzyl alcohol 1',4-disulfate), of the brown alga Ascophyllum nodosum (1,2,3,5-tetrahydroxybenzene 2,5-disulfate), and in rat urine (resorcinol mono- and disulfates). Detector response (254 nm) was linear within the approximate range from 30--125 ng to 5--10 microgram. Semipreparative scale chromatography provided sufficient amounts of purified phenol sulfates for further analysis by paper electrophoresis.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056890:Eukaryota / Q000032:analysis*; D008832:Microchemistry; D000459:Phaeophyceae / Q000032:analysis*; D010636:Phenols / Q000032:analysis* / Q000652:urine; D051381:Rats; D000461:Rhodophyta / Q000032:analysis*; D013464:Sulfuric Acids / Q000032:analysis* / Q000652:urine
D010636:Phenols; D013464:Sulfuric Acids