Hybridoma antibody immunoassays for the detection of parasitic infection: development of a model system using a larval cestode infection in mice.
A prototype immunodiagnostic assay has been developed using chronic infection with the larval cestode, Mesocestoides corti, as a model system in mice. The assay is highly sensitive, it appears to be absolutely specific for M. corti infection, and is based on the inhibition of binding (by sera from infected mice) of a radiolabelled anti-M. corti hybridoma antibody to a crude M. corti antigen extract. The hybridoma antibody binds to living M. corti larvae and is an IgG1 protein. In large scale experiments no false positives were detected and the only M. corti-infected mice not detected by the assay were hypothymic nude (nu/nu) mice. Only limited success has been achieved in attempts to convert the assay to one not requiring parasite antigen and based on the inhibition of binding of radiolabelled anti-parasite hybridoma antibody and a large pool of anti-idiotype antiserum. Monoclonal antibodies derived from anti-parasite antibody-secreting hybridoma cell lines will be of particular use in the development of new, highly specific, immunodiagnostic reagents for the detection of parasite infection, exposure and disease.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D002459:Cell Fusion; D002460:Cell Line; D002589:Cestoda / Q000276:immunology; D002590:Cestode Infections / Q000175:diagnosis*; D003937:Diagnosis, Differential; D004195:Disease Models, Animal; D005260:Female; D006822:Hybrid Cells / Q000276:immunology; D007814:Larva; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D051379:Mice; D010954:Plasmacytoma; D011863:Radioimmunoassay / Q000379:methods*; D013154:Spleen / Q000166:cytology
D000912:Antibodies, Neoplasm; D000954:Antigens, Surface