Interaction of certain bovine immunoglobulins with complement in a single radial haemolysis system.
Bovine IgG1, IgG2, and IgM initiated haemolysis by bovine complement. With guinea pig complement bovine IgG1 and IgM appeared effective, but bovine IgG2 was much less effective. Single radial haemolysis systems using guinea pig complement to measure bovine antibody are likely to detect predominantly IgG1 and IgM. However, in vivo IgG2 should activate bovine complement.
D000818:Animals; D002417:Cattle / Q000276:immunology*; D003167:Complement Activation; D003165:Complement System Proteins / Q000276:immunology*; D006461:Hemolysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D003165:Complement System Proteins