Gas-liquid chromatography of digitoxigenin and digoxigenin as acetates of their epoxygeninic acid methyl esters.
Gas chromatographic analysis of ditigoxigenin and digoxigenin, the genins of the cardenolide glycosides digitoxin and digoxin, cannot be done without derivatization. However, during the derivatization, side-reactions often present serious problems. A procedure has been found for transforming digitoxigenin and digoxigenin into the corresponding acetates of their epoxygeninic acid methyl esters, which are stable compounds and suitable for gas chromatographic analysis.
D000085:Acetates / Q000032:analysis; D002849:Chromatography, Gas; D004073:Digitoxigenin / Q000032:analysis*; D004076:Digoxigenin / Q000032:analysis*; D004077:Digoxin / Q000031:analogs & derivatives*; D004355:Drug Stability; D004852:Epoxy Compounds / Q000032:analysis; D004952:Esters / Q000032:analysis
D000085:Acetates; D004852:Epoxy Compounds; D004952:Esters; D004073:Digitoxigenin; D004077:Digoxin; D004076:Digoxigenin