Non-specific adsorption of interferon to immobilized serum immunoglobulin.
Human leukocyte interferon attaches to hyperimmune serum immunoglobulins, coupled to agarose, even though the antibodies are not specific for interferon. Unless it is first washed off with ethylene glycol, the attached interferon elutes together with immune-specific antigens during immuno-affinity chromatography.
D000327:Adsorption; D000906:Antibodies; D002846:Chromatography, Affinity; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D007372:Interferons / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D007136:Immunoglobulins; D007372:Interferons