Translational activity of messenger ribonucleic acid isolated from unstimulated and phytohaemagglutinin-activated lymphocytes.
Purified cytoplasmic poly(A)+ RNA isolated from unstimulated pig lymphocytes has the same ability to direct translation in a range of cell-free systems as the corresponding mRNA from 20h phytohaemagglutinin-activated lymphocytes. Additional methylation of the mRNA is not required for maximum protein synthesis in the wheat-germ cell-free system. Misleading results are obtained if the mRNA preparations used are not adequately purified, and a method suitable for routine assessment of the degree of purification achieved is described. Cell-free protein-synthesizing systems from unstimulated lymphocytes translate added lymphocyte mRNA with lower efficiency than do comparable systems from phytohaemagglutinin-activated lymphocytes, whatever the source of the mRNA used.
D000818:Animals; D002474:Cell-Free System; D003593:Cytoplasm / Q000032:analysis; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000032:analysis*; D010442:Peptide Chain Initiation, Translational; D010835:Phytohemagglutinins / Q000494:pharmacology; D011061:Poly A; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D013552:Swine
D010835:Phytohemagglutinins; D012333:RNA, Messenger; D011061:Poly A