Control of blood group specific substances A and B and human chorionic gonadotropin in human immunoglobulins and albumins.
One hundred and thirty-six lots of normal human immunoglobulins and 95 lots of human albumins were tested for blood group substances A and B using haemagglutination inhibition assay. Blood group substances A and B were revealed in all the samples of commercial preparations from placental origin. The haemagglutination inhibition test is very suitable for routine control but since the only standard is the U.S. standard for blood group substances A and B, an international standard for these substances is urgently needed. Sixty-five lots of normal human immunoglobulins and 15 lots of human albumins were assayed against the Second International Standard for Human Chorionic Gonadotropin (HCG) using three biological and three immunological methods. Hormonal activity was revealed in all the preparations from placental origin. The results obtained by immunological methods were found to be considerably higher than those obtained by biological methods which indicate that the activities measured by the two groups of methods were different. The control of preparations is discussed. Antibodies to HCG were found in the immunoglobulin preparations as well.
D000017:ABO Blood-Group System; D006063:Chorionic Gonadotropin / Q000097:blood*; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D011786:Quality Control; D012015:Reference Standards; D012016:Reference Values; D012709:Serum Albumin / Q000032:analysis*
D000017:ABO Blood-Group System; D006063:Chorionic Gonadotropin; D007136:Immunoglobulins; D012709:Serum Albumin