Analysis of azanaphthalenes and their enzyme oxidation products by high-performance liquid chromatography, infrared spectroscopy and mass spectrometry.
Adsorption and reversed-phase high-performance liquid chromatography (HPLC) have been successfully used to separate metabolites from the parent heterocycles (isoquinoline, 3-methylisoquinoline, phthalazine, quinazoline, quinoxaline and cinnoline). Retention data are reported. The metabolites, hydroxyazanaphthalenes, which arise as a result of aldehyde oxidase catalysed oxidation, could be extracted in microgram quantities from incubation mixtures by n-butanol. Complete identification of the oxidation products was achieved by collecting fractions corresponding to each compound from the HPLC eluate and subjecting these to infrared and mass-spectral analysis.
D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D008099:Liver / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D009281:Naphthalenes / Q000032:analysis*; D010084:Oxidation-Reduction; D011817:Rabbits; D013055:Spectrophotometry, Infrared / Q000379:methods
D009281:Naphthalenes; D000445:Aldehyde Oxidoreductases