title: Isolation of human germ-line DNA suitable for recombinant DNA studies.
abstract: Dna from human sperm cells can be isolated with size and purity sufficient for use in recombinant DNA research. The DNA averages 100 000 base pairs (bp) in size (about 70.10(6) daltons) and is free of somatic cell, bacterial, and viral DNA. It can therefore be cloned under P2 + EK2 conditions as stipulated in the 1978 NIH Guidelines for recombinant DNA research.
mesh_terms: D002499:Centrifugation, Density Gradient; D003001:Cloning, Molecular; D003264:Containment of Biohazards; D004247:DNA / Q000302:isolation & purification*; D004274:DNA, Recombinant; D006801:Humans; D008297:Male; D008970:Molecular Weight; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D004274:DNA, Recombinant; D004247:DNA