Temperature dependence of 31P-NMR spectra of chicken erythrocyte nucleosomes.
The shape of the 31P-NMR signal of DNA in nucleosomes isolated from chicken erythrocytes has been examined in the temperature range of 15--80 degrees C at 40.48 MHz. This resonance is composed of at least two partially overlapping peaks with different line-widths and chemical shifts. The relative intensities of the peaks were found to vary with temperature. Although a quantitative analysis of the components is difficult at this frequency, the presence of various peaks under the resonance signal is further confirmed by inspection of the partially relaxed spectra at 15 degrees C. A comparison of the thermal behavior of protein-free DNA isolated from nucleosomes with that of DNA bound to histones indicates that DNA segments in nucleosomes take on several conformational states.
D000818:Animals; D002645:Chickens; D004247:DNA / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D009707:Nucleosomes / Q000032:analysis*; D013696:Temperature
D009707:Nucleosomes; D004247:DNA