Convenient method for the gas chromatographic analysis of hexosamines in the presence of neutral monosaccharides and uronic acids.
A convenient gas chromatographic method has been devised for the analysis of hexosamines in the presence of neutral and acidic sugars, which involves sequential derivatization reactions of nitrous acid deamination, mercaptalation, and trimethylsilylation. This method allows rapid, simultaneous determination of 0.1-1 micromole samples of hexosamines with coefficients of variation of less than 3%.
D000328:Adult; D000818:Animals; D001943:Breast Neoplasms / Q000652:urine; D002356:Cartilage / Q000032:analysis; D002849:Chromatography, Gas; D005260:Female; D006595:Hexosamines / Q000032:analysis* / Q000652:urine; D006801:Humans; D006868:Hydrolysis; D009005:Monosaccharides; D009077:Mucins / Q000032:analysis; D013552:Swine; D014470:Umbilical Cord / Q000032:analysis; D014574:Uronic Acids; D014907:Whales
D006595:Hexosamines; D009005:Monosaccharides; D009077:Mucins; D014574:Uronic Acids