Gas-liquid chromatography of some alkyl derivatives of 5-fluorouracil.
Various procedures for converting 5-fluorouracil into its methyl, butyl and hexyl derivatives are described. Structures were established as the N,N'-dialkyl derivatives using mass spectrometry or combined gas-liquid chromatography-mass spectrometry. The reaction conditions, i.e., the amount of derivatization reagents and reaction time, were optimized. Gas-liquid chromatographic characteristics of the derivatives were investigated on different stationary liquid phases, and 2% or 3% SP-2250, 5% XE-60 and 5% OV-1 were found to be superior. With 5-chlorouracil as the internal standard a linear response for the various derivatives was observed in the microgram range. The applicability of the different dialkyl derivatives in the measurement of 5-fluorouracil in biological materials is discussed.
D000478:Alkylation; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D005472:Fluorouracil / Q000032:analysis* / Q000097:blood; D006801:Humans; D013058:Mass Spectrometry; D008722:Methods; D008745:Methylation
D005472:Fluorouracil