Dissociation of rat colonic mucosa into single epithelial cells and their microscopic visualization.
Repeated incubation of rat colonic mucosa with phosphate-buffered mannitol yields morphologically intact single cell suspensions which are easily visualized microscopically with trypan blue. Cell dimensions are determined by means of a Nikon microcomparator.
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology*; D004847:Epithelial Cells; D005260:Female; D007413:Intestinal Mucosa / Q000166:cytology*; D008297:Male; D051381:Rats; D014343:Trypan Blue
D014343:Trypan Blue