[Biochemical studies on histones of the central nervous system. I. Characterization and partial identification of the label of histones from rat brain following intraventricular administration of 1-[14C]-acetate].
Rat brain histones were acetylated in vivo by intraventricular injection of [14C]-acetate. More than 90% of the label is the result of a true acetylation. Enzymatic proteolysis of the labelled histone fraction and subsequent chromatographic investigation of the digestion products showed about 60% of the recovered radioactive material to be epsilon-acetyl lysine, whereas 22% of the radioactivity was found in an unidentified spot.
D000085:Acetates / Q000378:metabolism; D000107:Acetylation; D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002250:Carbon Radioisotopes; D006657:Histones / Q000032:analysis*; D007553:Isotope Labeling; D008297:Male; D011402:Pronase; D051381:Rats; D014357:Trypsin
D000085:Acetates; D002250:Carbon Radioisotopes; D006657:Histones; D014357:Trypsin; D011402:Pronase
Biochemische Untersuchungen an Histonen des Zentralnervensystems. I. Charakterisierung und partielle Identifikation der Markierung von Histonen des Rattenhirns nach intraventrikulärer Gabe von 1-[14C]-Azetat.