Microcapillary agglutination assay for detection of specific antileukocyte reactivity in neutropenic patients.
Serum leukoagglutinating activity against the leukocytes of four patients with neutropenia was demonstrated using a modified microcapillary agglutination test. Cells from a panel of donors proved useful in attempting to define the identity of the antigens involved. In one instance anti-HLA-A9 activity could be demonstrated in a patient possessing HLA-A9. In the other three individuals no definite antigen assessment to HLA Series A and B antigens or the Lalezari series of neutrophil antigens could be made. Two of the patients' sera showed cross-reactivity and may be reactive with the same antigen or antigenic group. The microcapillary agglutination test appears to be useful in the evaluation of possible cases of autoimmune neutropenia.
D000328:Adult; D000368:Aged; D000372:Agglutination Tests / Q000379:methods*; D000380:Agranulocytosis / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis*; D001803:Blood Transfusion; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009503:Neutropenia / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology
D000941:Antigens; D001323:Autoantibodies; D006680:HLA Antigens