Isolation and properties of a phage lytic for non-smooth Brucella organisms.
A phage for non-smooth cells of Brucella abortus was isolated from a mixture of three brucella-phages incubated with rough brucella cells in the presence of N-methyl-N'-nitro-N-nitrosoguanidine. It did not lyse smooth cells of Br. abortus strains nor those of other Brucella species, but during the course of its replication in rough organisms a small proportion of phage mutants were produced which were similar in properties to smooth-specific phages. The rough-specific phage, R, itself resembled Weybridge phage in its morphological and serological properties. Phage R was found to contain DNA and segregated into high and low density fractions, both with plaque-forming activity, on ultra-centrifugation in CsSO4 gradients. Unlike the smooth-specific phages it attached to heat-labile receptors present on non-smooth brucella cells.
D000327:Adsorption; D001434:Bacteriophage Typing; D001435:Bacteriophages / Q000032:analysis / Q000302:isolation & purification*; D002002:Brucella / Q000145:classification*; D002499:Centrifugation, Density Gradient; D007106:Immune Sera; D009500:Neutralization Tests; D011991:Receptors, Virus; D010948:Viral Plaque Assay; D014779:Virus Replication
D007106:Immune Sera; D011991:Receptors, Virus