Measurement of adriamycin (doxorubicin) and its metabolites in human plasma using reversed-phase high-performance liquid chromatography and fluorescence detection.
We describe a method for measuring adriamycin and its major metabolite, adriamycinol, in plasma, using reversed-phase high-performance liquid chromatography and fluorescence detection. The lower limit of detection is approximately 1 ng/ml for both compounds; within-day coefficients of variation are 3.6% and 4.4% for adriamycin and adriamycinol, respectively. A slight modification of this procedure also allows measurement of aglycone metabolites.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004317:Doxorubicin / Q000097:blood* / Q000378:metabolism / Q000627:therapeutic use; D005453:Fluorescence; D006801:Humans
D004317:Doxorubicin