title: Calmodulin-binding proteins from brain and other tissues.
abstract: The calmodulin contents of rabbit brain, lung, kidney and liver, of bovine aorta and uterus, and of chicken gizzard have been determined. 2. The calmodulin in all of these tissues has been shown to be present in the form of very stable complexes with several other proteins. 3. A calmodulin-binding protein of mol.wt. 22 000 has been purified in high yield from bovine brain. It has been shown to interact with calmodulin and rabbit skeletal-muscle troponin C in a Ca2+-dependent manner. 4. The 22 000-mol.wt. protein inhibits the activation of bovine brain phosphodiesterase by calmodulin, but has very little affect on the activation of myosin light-chain kinase. 5. Calmodulin-binding proteins of mol.wts. 140000, 77000 and 61000 have also been partially purified from rabbit brain by affinity chromatography and have been shown to interact in a Ca2+-dependent manner with calmodulin. 6. The apparent molecular weights of the calmodulin-calmodulin-binding protein complexes, determined by gel filtration in the presence of 6M-urea, have been shown to be similar for most of the mammalian tissues examined. 7. By using 125I-labelled calmodulin, similar complexes have been demonstrated in rabbit skeletal muscle, although they are present at much lower concentrations.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D008970:Molecular Weight; D010726:Phosphodiesterase Inhibitors / Q000378:metabolism; D011817:Rabbits; D014018:Tissue Distribution; D014336:Troponin / Q000032:analysis
chemical_list: D000596:Amino Acids; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D002352:Carrier Proteins; D010726:Phosphodiesterase Inhibitors; D014336:Troponin