Starch gel electrophoresis: an effective method for separation of pathogenic and nonpathogenic Naegleria strains.
Isoenzyme electrophoresis of 7 different enzyme systems was used to compare 24 strains of Naegleria fowleri and 6 strains of N. gruberi. The 30 strains could be grouped into 4 distinct categories based upon zymogram patterns. No interstrain band variation in all enzyme systems was demonstrated in pathogenic strains of N. fowleri. Three nonpathogenic high temperature-tolerant strains of Naegleria had similar zymograms. Four of the 5 remaining nonpathogenic Naegleria strains had no interstrain band variation. Based upon zymograms, the 22 pathogenic strains constitute a homogenous species. Similarly the high temperature-tolerant nonpathogenic strains formed a cohesive group. The remaining nonpathogenic strains could be separated into 2 groups.
D000135:Acid Phosphatase / Q000032:analysis; D000656:Amoeba / Q000145:classification / Q000201:enzymology / Q000472:pathogenicity*; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D002474:Cell-Free System; D004592:Electrophoresis, Starch Gel; D005969:Glutamate Dehydrogenase / Q000032:analysis; D006884:Hydroxybutyrate Dehydrogenase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D007931:Leucyl Aminopeptidase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D051379:Mice; D014774:Virulence
D007527:Isoenzymes; D006884:Hydroxybutyrate Dehydrogenase; D008291:Malate Dehydrogenase; D005969:Glutamate Dehydrogenase; D002265:Carboxylic Ester Hydrolases; D000135:Acid Phosphatase; D007931:Leucyl Aminopeptidase