title: Measurement of testosterone with a high-performance liquid chromatograph equipped with a flow-through  ultraviolet spectrophotometer.
abstract: A technique is described for measuring nanogram amounts of testosterone using high-performance liquid chromatography with detection by a flow-through spectrophotometer. The addition of a non-radioisotopic internal standard (4-androsten-11 beta-ol-3,17-dione) to the biological specimen automatically corrects for testosterone losses due to extraction and non-quantitative sample injection into the high-performance liquid chromatograph. This new method, which can be performed by inexperienced personnel, is shown to be rapid, precise, accurate and specific for testosterone.
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D008297:Male; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation; D013737:Testis / Q000378:metabolism; D013739:Testosterone / Q000032:analysis* / Q000378:metabolism
chemical_list: D013739:Testosterone