In vitro production of sterioid cataract in bovine lens. Part I: measurement of optical changes.
Fresh calf lenses were incubated in nutritive media, to which was added ouabain and dexamethasone phosphate at concentrations of 1 X 10(-4) M. At the end of a three day incubation period, cortical opacification developed in the control series of lenses as well as those lenses incubated in both ouabain and dexamethasone phosphate. Using a light transmission device which quantitates lens opacification, it was noted that dexamethasone produced a level of cortical opacification significantly greater than that of the control series. Ouabain produced a level of cortical opacification statistically identical to that produced by the dexamethasone. It is suggested that the aforementioned light transmission device is an accurate and reproducible method of quantitating cataractous opacification.
D000818:Animals; D002386:Cataract / Q000139:chemically induced*; D002417:Cattle; D003907:Dexamethasone / Q000494:pharmacology*; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000187:drug effects*; D010042:Ouabain / Q000494:pharmacology*; D010783:Photometry / Q000295:instrumentation; D012107:Research Design
D010042:Ouabain; D003907:Dexamethasone