Non-nucleolar transcription complexes of rat liver as revealed by spreading isolated nuclei.
Miller's technique was applied to isolated nuclei of rat liver. Both the usual nucleolar and non-nucleolar transcription complexes were visualized. In addition, an unusual type of putative non-ribosomal transcription unit was revealed. It was charcaterized by a high density of the lateral ribonucleoprotein (RNP) fibrils. Although these particular units exhibited a regular increase of fibril lengths, the length of the transcript-covered deoxyribonucleoprotein (DNP) fibres and the morphological aspect of the RNP fibrils distinguished them from the nucleolar 'Christmas-tree'-like figures. The linear and granular configuration of the transcripts and the absence of terminal knobs made them similar to non-nucleolar nascent RNP fibrils.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D004247:DNA; D003852:Deoxyribonucleoproteins; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins; D051381:Rats; D014158:Transcription, Genetic
D003852:Deoxyribonucleoproteins; D009698:Nucleoproteins; D004247:DNA