Rapid method for the assay of 4-aminobutyric acid (GABA), glutamic acid and aspartic acid in brain tissue and subcellular fractions.
The thin-layer electrophoretic separation at pH 4.8 of brain extracts and a procedure for fluorescent staining of the plates with fluorescamine are described for the rapid routine determination of 4-aminobutyric acid (GABA), glutamic acid and aspartic acid in brain extracts and in particulate fractions of brain tissue. Automated sample application, electrophoretic separation using two chambers, and quantitation by in situ fluorescence scanning allows the assay of 280 samples within three working days. The method is reproducible (S.D. less than 8% of the mean) within the range of 0.2--2 nmole per spot. The staining procedure can be applied to a variety of related analytical problems. The method has proved useful for the determination of the specific radioactivities of GABA, glutamic acid and aspartic acid in metabolic studies.
D000375:Aging; D000818:Animals; D001224:Aspartic Acid / Q000032:analysis*; D001921:Brain / Q000254:growth & development; D001923:Brain Chemistry; D002852:Chromatography, Ion Exchange / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004586:Electrophoresis / Q000379:methods; D005450:Fluorescamine; D005971:Glutamates / Q000032:analysis*; D008297:Male; D051381:Rats; D013347:Subcellular Fractions / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D005971:Glutamates; D001224:Aspartic Acid; D005450:Fluorescamine; D005680:gamma-Aminobutyric Acid