[Synthesis of tyrosine and 3,4-dihydroxyphenylalanine by bacteria Citrobacter freundii].
Among facultative-anaerobic bacteria utilizing formic acid, a large number of strains having tyrosine phenol lyase were found. The enzyme can catalyze synthesis of tyrosine and 3,4-dihydroxy phenyl alanine (DOPA) from pyruvate, ammonium and, accordingly, phenol and pyrocatechol. These strains were identified as Citrobacter freundii. Cell suspensions of the most active strains synthesized up to 75 g/l tyrosine for 12 hr, up to 86 g/l tyrosine for 24 hr, and up to 29 g/l DOPA for 42 hr. A medium containing yeast autolysate grown on hydrocarbons can be recommended to produce cells having a high tyrosine phenol lyase activity.
D000641:Ammonia / Q000378:metabolism; D002954:Citrobacter / Q000378:metabolism*; D004295:Dihydroxyphenylalanine / Q000096:biosynthesis*; D010636:Phenols / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D013045:Species Specificity; D014443:Tyrosine / Q000096:biosynthesis*; D014447:Tyrosine Phenol-Lyase / Q000378:metabolism
D010636:Phenols; D011773:Pyruvates; D014443:Tyrosine; D004295:Dihydroxyphenylalanine; D000641:Ammonia; D014447:Tyrosine Phenol-Lyase
Sintez tirozina i 3,4-dioksifenilalanina bakterĭen Citrobacter freundii.