[Effect of cultivation temperature on thermal stability of lipolytic enzymes of the fungus Rhizopus microsporus, UzLT-1].
The yield, activity and thermal stability of lipolytic enzymes obtained from the culture fluid filtrates of Rhizopus microsporus, UzLT-1, cultivated at 28, 38 and 48 degrees C (preparations I, II and III, respectively) were investigated. Maximal lipolytic activity was found in Prepartion II, and maximal yield and thermal stability in Preparation III. By disc electrophoresis and DEAE-cellulose chromatography, the presence of three lipolytically active enzymes in the preparations was demonstrated.
D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification* / Q000378:metabolism; D008053:Lipid Mobilization; D012233:Rhizopus / Q000201:enzymology*; D013696:Temperature
D008049:Lipase
Vliianie temperatury kul'tivirovaniia na termostabil'nost' lipoliticheskikh fermentov griba Rhizopus Microsporus, shtammu UzLT-1.