Fate of labeled choline administered intraruminally to pregnant ewes given manganese-deficient or -supplemented rations.
Tissue samples were taken from pregnant ewes and their 3-month fetuses 96 hours after intraruminal dosing of the ewes with tritiated choline. Starting 5 months before mating, one group of ewes had been given a manganese (Mn)-deficient ration and a second group had been given a Mn-supplemented (normal) ration. The results indicate that Mn depletion decreased the overall uptake of choline; the radioactivity of all tissues from the Mn-depleted group was lower than the radioactivity of the corresponding tissues from the group given a Mn-supplemented (normal) ration. The radioactivity of fetal tissues was also lower in the Mn-deficient group than it was in the Mn-supplemented group. Lower radioactivity concentrations were also observed in the whole plasma (sampled at various times after radioisotope dosing) and its lipid extract of Mn-depleted ewes than in the plasma and its lipid extract of Mn-supplemented ewes. There was no significant difference in the fatty acid composition of hepatic phosphatidyl choline between the two groups. However, the proportions of linoleic, stearic, and arachidonic acids were lower in fetal hepatic phosphatidyl choline than in maternal hepatic phosphatidyl choline.
D000821:Animal Feed; D000818:Animals; D002794:Choline / Q000008:administration & dosage / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D008345:Manganese / Q000378:metabolism* / Q000494:pharmacology; D011247:Pregnancy; D011270:Pregnancy, Animal; D012417:Rumen; D012756:Sheep / Q000378:metabolism*
D005227:Fatty Acids; D008345:Manganese; D002794:Choline