[Characteristics of human neutrophils obtained by the chamber method of "skin window"].
Viability, morphology and function of healthy human cells migrated into the chamber through the "skin window" were studied. After 18 to 20 hours there were 42.0 +/- 5.3 (x 10(6))/cm2 viable cells characterized by a high content of mature neutrophils (98.6 +/- 0.6%). Normal reactivity of migrated neutrophils was observed using the nitroblue tetrazolium reduction test. The chamber variant of the "skin window" method is recommended as a simple physiological technique for preparing pure populations of human neutrophils.
D002469:Cell Separation / Q000379:methods; D006801:Humans; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*
Kharakteristika neĭtrofilov cheloveka, poluchennykh kamernym metodom "kozhnogo okna".