Adaptation of the influenza virus A2-pol 29/69 to the cells of the Chinese hamster lung (CHL) and to the cells BHK-21.
In the course of research of the possibilities of adapting the influenza virus serotype A 2-Pol 29/69 to BHK-21 and CHL cells its production was established in the investigated combinations. An indication of virus replication is an increase in infectious and haemagglutination titres within 48 h after infection and gradual increase in haemadsorption. Observation of the influence of trypsin (10 microgram/ml) indicates that trypsin increases the capability of viruses to penetrate the cells. The cytopathic effect was not observed in cells infected with influenza virus.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D006389:Hemagglutinins, Viral / Q000032:analysis; D009980:Influenza A virus / Q000254:growth & development* / Q000276:immunology; D007668:Kidney / Q000382:microbiology; D014357:Trypsin / Q000378:metabolism; D014779:Virus Replication
D006389:Hemagglutinins, Viral; D014357:Trypsin