Inhibitory effect of ethanol on 5-hydroxytryptamine (serotonin) uptake in human blood platelets in vitro.
Ethanol in concentrations higher than 10(-2) M (or about 0.5% W/W) inhibited the uptake of 14C-5-hydroxytryptamine (serotonin) in human blood platelets in an artificial, protein-free medium, by a non-competitive mechanism. The inhibition was not influenced by the alcohol dehydrogenase inhibitor methylpyrazole (10(-3) M). In concentrations up to 0.1 M, ethanol had no effect on 5-HT efflux from the platelets. At higher concentrations, ethanol increased efflux. Inhibition of 5-HT uptake was found to increase progressively in the sequence methanol - ethanol - propanol - butanol.
D000433:1-Propanol / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D000440:Butanols / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D000432:Methanol / Q000494:pharmacology; D012701:Serotonin / Q000097:blood*
D000440:Butanols; D012701:Serotonin; D000431:Ethanol; D000433:1-Propanol; D000432:Methanol