Corticosteroid determination in skin preparations by a reaction rate method.
A reaction rate method for the determination of beta-methasone, betamethasone valerate, triamcinolone acetonide, and fluocinolone acetonide is described. The method is based on a modification of the widely accepted blue tetrazolium reaction. Analysis times of 30--70 sec are required. Relative standard deviations of 0.3--1.9% are obtained, and the analytical working curves are linear. Analysis of pharmaceutical skin preparations by the new method gave results that correlated well with the time-consuming standard equilibrium method. Analysis of betamethasone and betamethasone valerate mixtures by measuring absorbance values at two different times was performed also.
D000287:Administration, Topical; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D002626:Chemistry, Pharmaceutical; D005938:Glucocorticoids; D007700:Kinetics; D008722:Methods; D009824:Ointments / Q000032:analysis; D013778:Tetrazolium Salts; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D009824:Ointments; D013778:Tetrazolium Salts