title: Nonspecific esterases in normal human blood cells: characterization of isoenzymes by isoelectric focusing in polyacrylamide gel.
abstract: Using the high resolution technique of isoelectric focusing in polyacrylamide gel, isoenzymatic components of nonspecific esterase were resolved in esterase-rich extracts obtained from normal human monocytes, lymphocytes, granulocytes and platelets. In the case of each cell type, different isoenzymatic patterns of nonspecific esterase activity could be visualized. These studies extend the cytochemistry of nonspecific esterases by detection of previously undescribed isoenzymatic components, and suggest that the elaboration of nonspecific esterase isoenzymes may be a function of cellular differentiation.
mesh_terms: D001773:Blood Cells / Q000201:enzymology*; D002454:Cell Differentiation; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification
chemical_list: D007527:Isoenzymes; D004950:Esterases