Role of 24- and 28-hydroxylated intermediates in the metabolism of beta-sitosterol in the insect Tenebrio molitor.
1. [28-3H]Stigmast-5-ene-3 beta, 28-diol and [23,23,25-3H]stigmast-5-ene-3 beta, 24-diol were synthesized. 2. Each of the samples was mixed with beta-[4-14C]sitosterol and administered to Tenebrio molitor larvae. 3. The former compound is not utilized by the insect; the latter, although metabolized to 24(28)-ethylidene sterols and cholesterol, is not a beta-sitosterol metabolite. 4. The above results are discussed in relation to the mechanism of formation of the 24(28)-double bond in beta-sitosterol metabolism in T. molitor.
D006900:Hydroxylation; D007814:Larva / Q000378:metabolism; D008956:Models, Chemical; D010840:Phytosterols / Q000378:metabolism*; D012855:Sitosterols / Q000378:metabolism*; D013265:Stigmasterol / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000378:metabolism*; D013712:Tenebrio / Q000378:metabolism*
D010840:Phytosterols; D012855:Sitosterols; D013265:Stigmasterol