Effect of mercury nitrate on the histochemical activity of some dehydrogenases in primary cultures of rat kidney tubular cells.
Direct effect of sublethal and lethal doses of mercury nitrate on histochemical activity of G6PDH, LDH and SDH was investigated in primary cultures of rat tubular cells. Enzyme activities were studied histochemically after administration of mercury nitrate for periods extending from 5 min. to 24 hrs and also after 2,3,4 and 5 following days. The sublethal doses of mercury nitrate containing 1 microgram and 5 microgram of mercury were found to decrease histochemical activity of the studied dehydrogenases as early as 10 and 15 minutes. Their inhibitory effect was much stronger after 24 h and depended on the dose of mercury compound. The lethal doses of mercury nitrate containing 15 microgram and 20 microgram of mercury depressed the activity of dehydrogenases within 5 min. after administration. The loss of enzyme activities usually preceded the appearance of necrotic signs in the cultured cells. It was also found that the cultured cells of kidney tubules treated with sublethal doses of mercury nitrate were usually able to regain the normal level of the enzymic activity within 2-5 days.
D000818:Animals; D002478:Cells, Cultured; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors*; D006651:Histocytochemistry; D007684:Kidney Tubules / Q000201:enzymology*; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors*; D008628:Mercury / Q000494:pharmacology*; D051381:Rats; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors*; D013997:Time Factors
D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D008628:Mercury