title: Bacterial transformation using temperature-sensitive mutants deficient in peptidoglycan synthesis.
mesh_terms: D001419:Bacteria / Q000235:genetics*; D002118:Calcium / Q000494:pharmacology; D003001:Cloning, Molecular / Q000379:methods*; D009154:Mutation; D010457:Peptidoglycan / Q000096:biosynthesis / Q000235:genetics*; D013045:Species Specificity; D013696:Temperature; D014162:Transfection / Q000187:drug effects; D014170:Transformation, Genetic / Q000187:drug effects
chemical_list: D010457:Peptidoglycan; D002118:Calcium