High-performance liquid chromatographic determination of theophylline and its derivatives with electrochemical detection.
Theophylline and its derivatives are determined by high-performance liquid chromatographic separation on a C8 reversed-phase column with electrochemical detection. The analyte is oxidized at 1.24 V (vs. the standard calomel electrode) on a wax-impregnated graphite electrode using classical d.c. and current sampled d.c. modes, and the results obtained compared with those of a 254 nm ultraviolet (UV) detector. Polarographic detection offers greater sensitivity for theophylline than UV detection; further, interferences which hamper UV detection may be selectively eliminated with polarographic detection.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004566:Electrodes; D011048:Polarography; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods; D013329:Structure-Activity Relationship; D013806:Theophylline / Q000031:analogs & derivatives* / Q000032:analysis*
D013806:Theophylline