Purine metabolism in rat skeletal muscle.
1. Rat skeletal muscle can synthesize purine nucleotides de novo. 2. Nucleotide synthesis de novo was inhibited by approximately 90% in the presence of 1 mM azaserine. 3. Purine nucleotides can be synthesized via "salvage" pathways from purine bases and nucleosides. Under the conditions employed incorporation rates were adenosine adenine inosine/hypoxanthine guanosine guanine. 4. Xanthine was not utilized for nucleotide synthesis.
D000818:Animals; D001377:Azaserine / Q000494:pharmacology; D002250:Carbon Radioisotopes; D005561:Formates / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D011687:Purines / Q000378:metabolism*; D051381:Rats; D012265:Ribonucleotides / Q000096:biosynthesis
D002250:Carbon Radioisotopes; D005561:Formates; D011687:Purines; D012265:Ribonucleotides; D001377:Azaserine; D005998:Glycine