Release of endogenous serotonin from "encéphale isolé" cats. I - Technical aspects.
Ventricular perfusion, push-pull cannulae at the caudate level and cortical superfusion were used in "encéphale isolé" cats in order to estimate endogenous 5-HT release. The amine was measured using the radioenzymatic assay. These different methods are described and compared. Technical aspects and advantages of cortical superfusion under physiological conditions are discussed.
D000818:Animals; D002415:Cats; D002540:Cerebral Cortex; D002552:Cerebral Ventricles; D002555:Cerebrospinal Fluid; D003010:Clorgyline / Q000494:pharmacology; D003655:Decerebrate State / Q000378:metabolism*; D002523:Edible Grain / Q000187:drug effects / Q000378:metabolism; D005260:Female; D008297:Male; D010293:Pargyline / Q000494:pharmacology; D010477:Perfusion / Q000379:methods; D011189:Potassium Chloride / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism*; D013696:Temperature
D012701:Serotonin; D011189:Potassium Chloride; D010293:Pargyline; D003010:Clorgyline