Rapid assay for repair of damage induced by ultraviolet light in bovine lymphocytes.
Bovine peripheral blood lymphocytes exhibit a peak of deoxyribonucleic acid (DNA) repair, measured after exposure of cells to ultraviolet light, three to four days after phytohaemagglutinin addition to diluted blood cultures. This peak of DNA repair is induced coincidentally with that of DNA replication. DNA repair is determined in the presence of hydroxyurea which inhibits DNA replication. This assay provides a rapid screening method for deficits in DNA repair synthesis in cattle.
D000818:Animals; D002417:Cattle / Q000097:blood*; D004260:DNA Repair; D004261:DNA Replication / Q000528:radiation effects; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays