Isolation of chromatin subcore particles by chromatography on Biogel 1.5 m.
The method is described for separation and purification by chromatography on Biogel 1.5 m of subnucleosomal nucleoprotein particles obtained by extensive digestion of calf thymus nuclei with micrococcal nuclease.
D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000302:isolation & purification*; D002850:Chromatography, Gel / Q000379:methods*; D004247:DNA / Q000302:isolation & purification; D008836:Micrococcal Nuclease / Q000494:pharmacology; D009707:Nucleosomes / Q000032:analysis*; D013950:Thymus Gland / Q000032:analysis* / Q000648:ultrastructure
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA; D008836:Micrococcal Nuclease