Celite column chromatography followed by reversed-phase high-performance liquid chromatography: a simple, two-step method for separating 14 testicular steroids.
The separation of 14 major testicular steroids has been effected by a simple, two-step procedure involving Celite column chromatography followed by reversed-phase high-performance liquid chromatography. The technique does not alter the resolved steroids, which are then available for subsequent analysis.
D002851:Chromatography, High Pressure Liquid; D007692:Diatomaceous Earth; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D012997:Solvents; D013256:Steroids / Q000302:isolation & purification*; D013735:Testicular Hormones / Q000302:isolation & purification*
D007202:Indicators and Reagents; D012997:Solvents; D013256:Steroids; D013735:Testicular Hormones; D007692:Diatomaceous Earth