Sensitive multi-residue method for detection of anabolics in urine and in tisssues of slaughtered animals.
A routine procedure is described for the dependable detection of various anabolic residues in tissues or urine contaminated at levels as low as 0.5-10 ppb (10 parts per 10(9)). A suitable extraction and clean-up procedure was developed, permitting adequate recovery (60-80%) of various anabolics from tissue samples (50 g) or urine (50 ml). Following two-dimensional thin-layer chromatography, the presence of the anabolic residues are detected by sulphuric acid-induced fluorescence. The detection limit of most anabolics is of the order of 1-10 ng.
D000728:Androgens / Q000032:analysis*; D000818:Animals; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods; D004967:Estrogens / Q000032:analysis*; D011372:Progestins / Q000032:analysis*; D013256:Steroids / Q000652:urine; D013329:Structure-Activity Relationship
D000728:Androgens; D004967:Estrogens; D011372:Progestins; D013256:Steroids