Induction of cell-mediated cytotoxicity by lipoprotein containing histocompatibility antigens.
Lipoprotein was isolated from tumour cells by sonication and ultracentrifugal flotation on KBr gradients. It contained H-2 antigen detectable by antibody binding and induced a primary or secondary cell-mediated cytotoxic response in vitro which was H-2 specific. In a syngeneic model only a secondary cell-mediated response was stimulated and no competitive inhibition of the effector step of cell-mediated lysis could be demonstrated. The implications of these findings are discussed.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D006183:H-2 Antigens; D007156:Immunologic Memory; D007694:Killer Cells, Natural / Q000276:immunology; D008074:Lipoproteins / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000276:immunology; D013347:Subcellular Fractions / Q000276:immunology; D014461:Ultracentrifugation
D006183:H-2 Antigens; D008074:Lipoproteins