Nucleosome dissociation and transfer in concentrated salt solutions.
We have examined the dissociation of nucleosomes into histones and free, 4.5S DNA over a range of sodium chloride concentrations between 0.25 and 1 M. We have also studied this dissociation as a function of nucleosome concentration at two salt concentrations, 0.8 M and 0.9 M. In addition, we have measured the kinetics of transfer of histone cores from nucleosomes onto recipient bacteriophage T7 DNA in 0.6, 0.7 and 0.8 M NaCl solutions. Although the mechanism of nucleosome transfer is unknown the data presented here are consistent with either a reversible dissociation of the nucleosome or DNA strand displacement by another DNA.
D000818:Animals; D002645:Chickens; D004247:DNA / Q000032:analysis*; D004279:DNA, Viral / Q000302:isolation & purification; D004912:Erythrocytes / Q000032:analysis; D006657:Histones / Q000032:analysis*; D007700:Kinetics; D009707:Nucleosomes / Q000032:analysis*; D009994:Osmolar Concentration; D011485:Protein Binding; D013604:T-Phages / Q000032:analysis
D004279:DNA, Viral; D006657:Histones; D009707:Nucleosomes; D004247:DNA