[Simian adenovirus type 7 (SA7) DNA fragmentation by specific R.Bgl II and R.Hind III endonucleases].
The effect of restricting R. BgIII and R. HindIII endonucleases on the genome of simian adenovirus type 7 (SA7) was studied. The R. BgIII endonuclease has 5 restriction sites on SA7 DNA and produces 6 fragments with molecular weights of 10.0 X 10(6), 5,3 X 10(6), 3.6 X 10(6), 1.8 X 10(6), 0.6 X 10(6), and 0.5 X 10(6) daltons. R. HindIII, having 3 restriction sites, hydrolyses SA7 DNA into 4 fragments with molecular weights of 7.6 X 10(6), 5.9 X 10(6), 5,2 X 10(6), 3,1 X 10(6) daltons. The R. BgIII and R. HindIII fragments are arranged in the SA7 genome in the following order: B-A-C-E-D-F and C-D-B-A.
D000256:Adenoviridae / Q000648:ultrastructure*; D000261:Adenoviruses, Simian / Q000648:ultrastructure*; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis / Q000378:metabolism*; D005814:Genes, Viral / Q000187:drug effects; D006868:Hydrolysis; D008970:Molecular Weight
D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Fragmentatsiia DNK adenovirusa obez'ian tipa 7 (SA7) spetsificheskimi éndonukleazami R.Bgl II i R.Hind III.