A microtitre technique for the assay of malignant catarrhal fever virus and neutralising antibody.
A microtitre technique for the quantal assay of a cell-free strain of malignant catarrhal fever virus was developed, using serially passaged bovine embryonic kidney cells. End-points were determined after 12 days' incubation and the mean titre recorded for a single virus stock stored at -70 degrees C over a six-month period was 10(5.5) +/- 0.2 (SD). In neutralisation tests serum/virus mixtures were best held at 37 degrees C for 1 h in microtitre trays before the addition of cells; assays were highly reproducible, figures of 10(1.5) +/- 0.2 being obtained for a single reference serum.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D006564:Herpesviridae / Q000254:growth & development / Q000276:immunology; D008304:Malignant Catarrh / Q000382:microbiology*; D009500:Neutralization Tests; D011817:Rabbits; D014776:Virus Cultivation
D000914:Antibodies, Viral