Determination of edrophonium chloride injection USP in the presence of preservatives.
The determination of Edrophonium Chloride Injection involves a modification of a procedure for phenylephrine and offers an alternative to the compendial assay. Edrophonium chloride forms an ion-pair with di-(2-ethylhexyl) phosphoric acid which can be extracted with an immiscible solvent such as ether. When this procedure is coupled with partition chromatography, the drug can be isolated from preservatives and quantitatively determined by its ultraviolet absorbance. Recovery data on synthetic mixtures ranged from 99.8 to 101.9% and were in good agreement with the compendial method.
D004491:Edrophonium / Q000032:analysis*; D007267:Injections; D011310:Preservatives, Pharmaceutical; D012996:Solutions / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D011310:Preservatives, Pharmaceutical; D012996:Solutions; D004491:Edrophonium