Isolation and characterization of DNA-dependent RNA polymerases from rabbit mammary gland.
RNA polymerases AI, AII, BI, BII, CI and CII were found in the mammary gland from lactating rabbits. The enzymes obtained from total cell homogenates were partially purified and separated by DEAE-Sephadex chromatography. Their chromatographic properties, alpha-amanitin-sensitivity, template specificity, ionic strength and divalent cation requirements are described.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology; D002852:Chromatography, Ion Exchange / Q000379:methods; D003593:Cytoplasm / Q000201:enzymology*; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification*; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000648:ultrastructure; D009691:Nucleic Acid Denaturation; D009994:Osmolar Concentration; D011247:Pregnancy; D011817:Rabbits; D013950:Thymus Gland
D000546:Amanitins; D012321:DNA-Directed RNA Polymerases