The effect of in vitro irradiation on PHA-mediated cytotoxicity and lymphocytes with receptors for the Fc part of Ig.
In vitro irradiation of purified human lymphocytes reduced PHA-mediated cytotoxicity (MICC) against chicken erythrocytes in a dose-dependent fashion. Lymphocytes with receptors for IgG present in both unfractionated and T-cell enriched cell preparations were depleted after X-ray exposure. T-cells with IgM-receptors, however, appeared to be more resistant. Different lymphocyte subpopulations seem to cooperate in MICC, and shifts of their proportions may be one explanation of the reduction of MICC after X-ray exposure.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000528:radiation effects; D001943:Breast Neoplasms / Q000532:radiotherapy; D002645:Chickens; D003602:Cytotoxicity, Immunologic / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011961:Receptors, Fc; D012397:Rosette Formation; D013997:Time Factors
D010835:Phytohemagglutinins; D011961:Receptors, Fc