[Using a combined transcription-translation system for determining the role of cryptic plasmids of Bacillus thuringiensis].
The possibility of entomocyde crystal protein synthesis was studied using a heterological cell-free system with Bacillus thuringiensis plasmid DNA as template. The high level of template activity is usual for Bac. thuringiensis plasmid DNA. Immunochemical studies of the in vitro synthesized polypeptides showed that Bac. thuringiensis plasmid DNA does not direct crystal protein synthesis.
D001413:Bacillus thuringiensis / Q000378:metabolism*; D007700:Kinetics; D010452:Peptide Biosynthesis; D010957:Plasmids; D014176:Protein Biosynthesis; D013698:Templates, Genetic; D014158:Transcription, Genetic
Ispol'zovanie sovmeshchenoĭ sistemy transkriptsii--transliatsii dlia vyiusneniia roli kripticheskikh plazmid Bacillus thuringiensis.