Cyclical transmission of Trypanosoma brucei rhodesiense and Trypanosoma congolense by tsetse flies infected with culture-form procyclic trypanosomes.
Culture procyclic forms of Trypanosoma brucei rhodesiense and Trypanosoma congolense were fed to Glossina morsitans morsitans through artificial membranes. A very high percentage of the flies so fed produced established midgut infections, a proportion of which went on to develop into mature metacyclic trypanosomes capable of infecting mammalian hosts. The method offers a safe, clean way of infecting tsetse flies with African trypanosomes which reduces the need for trypanosome-infected animals in the laboratory.
D000818:Animals; D001769:Blood; D005247:Feeding Behavior; D007303:Insect Vectors / Q000469:parasitology*; D007422:Intestines / Q000469:parasitology; D051379:Mice; D013045:Species Specificity; D014345:Trypanosoma / Q000254:growth & development*; D014346:Trypanosoma brucei brucei / Q000254:growth & development*; D014353:Trypanosomiasis, African / Q000635:transmission*; D014370:Tsetse Flies / Q000469:parasitology*