[Presence of genes of ribosomal and transfer RNAs in kinetoplast DNA from two Crithidia species].
The coding properties of kinetoplast DNA from two respresentatives of the order Kinetoplastidae--Crithidia oncopelti and C. fasciculata--were studied. Experiments on hybridization of the whole network and fraction of minicircles with labelled 23S and 16S rRNA and with tRNA isolated from kinetoplasts of C. oncopelti clearly demonstrated the presence of the genes for these RNAs in the whole network and their absence in the minicircles. It may be thus concluded that the genes of ribosomal and transfer RNAs are localized in the maxicircular molecules. Similar efficiency of hybridization of rRNAs from C. oncopelti with kDNA from C. fasciculata and C. oncopelti revealed significant conservativity of ribosomal genes in the protozoa under study.
D000818:Animals; D003421:Crithidia / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005796:Genes; D007700:Kinetics; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000096:biosynthesis*; D013045:Species Specificity; D014158:Transcription, Genetic
D012335:RNA, Ribosomal; D004247:DNA; D012343:RNA, Transfer
O nalichii genov ribosomnykh i transportnykh RNK v kinetoplastnoĭ DNK dvukh vidov kritidiĭ.