Photoelectrode with a very short time-constant for recording intracerebrally Ca2+ transients at a cellular level.
In order to study calcium transients in the central nervous system of vertebrates a microphotoelectrode was designed. It is composed of three channels, through the first aequorin is perfused into the extracellular space under constant pressure, the second is an optical glass light-conducting channel, and the third a classical NaCl-filled recording pipette. The light emitted by the interaction of the injected aequorin with the local extracellular calcium is transmitted through the optic fiber to a photomultiplier of high sensitivity.
D000331:Aequorin / Q000008:administration & dosage*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002415:Cats; D002490:Central Nervous System / Q000378:metabolism*; D008164:Luminescent Proteins / Q000008:administration & dosage*; D008839:Microelectrodes; D008845:Microinjections / Q000295:instrumentation*; D009448:Neurochemistry / Q000295:instrumentation*; D010406:Penicillins; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism
D008164:Luminescent Proteins; D010406:Penicillins; D000331:Aequorin; D002118:Calcium