title: Cytotoxicity of human mononuclear cells against chicken and human red blood cells, induced by treatment of the effector cells with phospholipase C.
abstract: Human mononuclear cells from peripheral blood which were treated with phospholipase C (PLC), became cytotoxic against human or chicken red blood cells. PLC-induced cellular cytotoxicity against human red blood cells was further analyzed and compared to anti-D-mediated, antibody-dependent cellular cytotoxicity (ADCC), using the same target cells. ADCC, but not cytotoxicity of PLC-treated effector cells, was inhibited by free IgG. In addition, iodoacetate strongly enhanced PLC-induced cytotoxicity, but blocked ADCC completely. Addition of fetal calf serum or human AB serum impaired PLC-induced cytotoxicity. A similar inhibition was found by adding lecithin liposomes suggesting that the inhibitory effect of sera was also due to their phospholipid content. The data show that cytotoxicity of PLC-treated effector cells can be clearly distinguished from cellular cytotoxicity, occurring spontaneously or induced by target cell antibodies. We favor the notion that cytotoxicity of PLC-treated effector cells against human erythrocytes is due to the action of PLC, adsorbed to the effector cells.
mesh_terms: D000042:Absorption; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D001769:Blood; D002645:Chickens; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007461:Iodoacetates / Q000494:pharmacology; D010586:Phagocytes / Q000276:immunology*; D010713:Phosphatidylcholines / Q000494:pharmacology; D010740:Phospholipases / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000494:pharmacology*
chemical_list: D007461:Iodoacetates; D010713:Phosphatidylcholines; D010740:Phospholipases; D010738:Type C Phospholipases