Effect of influenza infection on the phagocytic and bactericidal activities of pulmonary macrophages.
The effect of mouse-adapted influenza A/PR/8/34 virus on pulmonary macrophage function was evaluated by using an in vitro system which allowed direct virus interaction with macrophages and then separate analysis of the steps required for bacterial clearance by macrophages. Infection of macrophages with this virus resulted in the appearance of a hemagglutinating activity on the macrophage surface; expression of this activity was inhibited by amantadine, 2-deoxyglucose, and cycloheximide and by pretreatment of the virus inoculum with ultraviolet light and specific antiserum. Since there was no release of extracellular virus, this growth cycle appeared to be incomplete (abortive). After influenza infection, net ingestion of viable Staphylococcus aureus by macrophage monolayers was unaltered and there was no change in the fraction of the monolayer which ingested cocci over a wide range of bacterial inputs. Influenza-infected macrophages also inactivated intracellular S. aureus at a rate indistinguishable from controls. Therefore, these in vitro studies do not support the hypothesis that the defect in pulmonary antibacterial mechanisms associated with influenza infections results from a direct effect of virus infection on either the phagocytic or bactericidal activity of resident pulmonary macrophages.
D000547:Amantadine / Q000494:pharmacology; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D003847:Deoxyglucose / Q000494:pharmacology; D005260:Female; D006388:Hemagglutinins / Q000096:biosynthesis; D007106:Immune Sera / Q000494:pharmacology; D066298:In Vitro Techniques; D009980:Influenza A virus / Q000254:growth & development; D008168:Lung / Q000166:cytology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D010587:Phagocytosis; D013211:Staphylococcus aureus / Q000276:immunology*; D014466:Ultraviolet Rays; D014779:Virus Replication
D006388:Hemagglutinins; D007106:Immune Sera; D003513:Cycloheximide; D003847:Deoxyglucose; D000547:Amantadine