A method for micro-scale isolation of sulfated glycopeptide from tissue.
Micro-scale isolation of sulfated glycopeptide from tissue was achieved by successive application of pronase digestion (Step 1), cetylpyridinium chloride-fractionation (Step 2), crude heparinase digestion or chondroitinase ABC digestion plus nitrous acid treatment (Step 3) and preparative cellulose acetate membrane-electrophoresis (Step 4). By this method, sulfated glycopeptide was obtained in a high yield from estrogen-treated rabbit uterus.
D000818:Animals; D004586:Electrophoresis; D005260:Female; D006020:Glycopeptides / Q000302:isolation & purification*; D008832:Microchemistry / Q000379:methods; D011817:Rabbits; D013431:Sulfates / Q000302:isolation & purification; D014599:Uterus / Q000032:analysis
D006020:Glycopeptides; D013431:Sulfates