Isolation by high-performance liquid chromatography and quantitation by radioimmunoassay of therapeutic concentrations of digoxin and metabolites.
A method combining high-performance liquid chromatography for separation of digoxin from three of its metabolites with subsequent quantitation of each compound by radioimmunoassay is described. The metabolites are shown to interfere with the radioimmunoassay procedure thus providing the need for separation prior to assay.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004077:Digoxin / Q000032:analysis* / Q000378:metabolism / Q000627:therapeutic use; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods
D004077:Digoxin