Reduction of Cu2+ by isolated rat liver mitochondria.
The present work provided evidence that isolated rat liver mitochondria reduce the larger part of the added CU2+ and that the reduction is of important for copper accumulation in mitochondria. The process is rapid, energy-independent and affected by steric factors. Both mitochondrial thiols and cytochrome c could participate in the Cu2+-reduction. Blocking of these mitochondrial components decrease sharply but does not abolish the reduction ability of mitochondria. Copper ions undergoing reversible reduction and oxidation in the presence of cysteine and ferrocytochrome c lead to the formation of O2- and H2O2. Superoxide radicals arise only from reoxidation of Cu+.
D000818:Animals; D002413:Cations, Divalent; D003300:Copper / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D013997:Time Factors
D002413:Cations, Divalent; D003300:Copper