The migration of fibroblasts into an in vitro wound.
A method for the study in vitro of fibroblast migration in wounds is described. This consists of producing standard fibroblast monolayers (derived from subcultures of explants of flank skin from young male rats) in microtitre plates, wounding the monolayer in a linear fashion with razor blade, removing the monolayer from one side of the linear wound and retaining the other side for migration studies. Preliminary results using the method are described which illustrate its reproducibility and ease of performance.
D000818:Animals; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003470:Culture Media; D005347:Fibroblasts / Q000502:physiology*; D008297:Male; D008937:Mitomycins / Q000494:pharmacology; D051381:Rats; D012867:Skin / Q000166:cytology; D013696:Temperature; D013997:Time Factors; D014945:Wound Healing
D003470:Culture Media; D008937:Mitomycins; D003078:Colchicine