Divalent cation transport in kidney slices. I. Properties of calcium transport in slices of rat kidney cortex and the effects of diuretics.
Correlative studies on transports of Ca2+ and Na+ and the properties of Ca2+ transport were carried out in rat kidney cortex slices. Ouabain had no effect on the Ca2+ transport but did inhibit the Na+ transport extensively. With addition of 2,4-dinitrophenol to the incubation medium, or under the anaerobic conditions, the effluxes of Ca2+ and Na+ were inhibited while the Ca2+ influx was enhanced significantly. Sulfhydryl inhibitors such as ethacrynic acid, mersalyl and p-chloromercuribenzoic acid suppressed Ca2+ efflux and stimulated Ca2+ influx. When the slices of kidney cortex were treated with these inhibitors, there was a reduction in the content of cellular ATP. The present results suggest that Ca2+ transport may be partly independent of Na+ transport, and that Ca2+ efflux may require energy-yielding processes.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology*; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D051381:Rats; D012964:Sodium / Q000378:metabolism
D002413:Cations, Divalent; D002729:Chloromercuribenzoates; D004232:Diuretics; D000255:Adenosine Triphosphate; D012964:Sodium; D002118:Calcium