[Isolation and some properties of homogenous nuclease S1 from alpha-amyloryzin].
A purification method for isolating homogeneous single-strand specific nuclease S1 from alpha-amyloryzin has been developed. The yield was about 16% and purification factor--9000. Nuclease S1 thus obtained was proved to be free of contaminations of any others nucleolytic enzymes. It is shown for the first time that ribo- and deoxy-dinucleosidemonophosphates are hydrolyzed by nuclease S1 to form 5'-nucleotides with pH optimum for ApA equal to 4.6.
D001236:Aspergillus oryzae / Q000201:enzymology; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012260:Ribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012260:Ribonucleases
Poluchenie i nekotorye kharakteristiki funktsional'no gomogennogo preparata nukleazy S1 iz al'fa-amilorizina.