Histochemical demonstration of G6PDH- and SDH-activity on cultured fibroblastoid cells from rat pancreas using a "membrane technique".
The applied "membrane technique" represents a simple and time-saving method to provide evidence for presence of enzyme activities in single cultured cells. Substrates for G6PDH and SDH are filled on a semipermeable membrane stretched over a cylinder of plastic. The cells are cultured on a polyester foil and incubated for the enzyme staining reaction on the membrane. Enzyme products can be visualized under the microscope within the cells in definite areas.
D000818:Animals; D005347:Fibroblasts / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods*; D008567:Membranes, Artificial; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D013385:Succinate Dehydrogenase / Q000032:analysis*
D008567:Membranes, Artificial; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase