Failure of tumor promoter 12-O-tetradecanoylphorbol-13-acetate to displace estradiol from its specific receptor.
A permanent cell line derived from rat endometrium which contains a specific, low capacity, high affinity estrogen binding protein in cytosol and nuclear fractions (estrogen receptor) is available. Extracts of cells from this line did not appreciably bind the tumor promoter, 12-0-tetradecanoylphorbol-13-acetate. The result suggests that the action of the promoter does not involve translocation to the cell nucleus via binding to the specific estrogen receptor.
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004717:Endometrium / Q000187:drug effects*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D010704:Phorbols / Q000494:pharmacology*; D051381:Rats; D011960:Receptors, Estrogen / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002273:Carcinogens; D010704:Phorbols; D011960:Receptors, Estrogen; D004958:Estradiol; D013755:Tetradecanoylphorbol Acetate