The collagen substratum influences in vitro hatching and attachment of the mouse blastocyst in a serumless medium.
When a serumless medium is used for the in vitro growth and development of post-blastocyst mouse embryos, a collagen substratum causes a delay in the hatching from the zona pellucida. However, the collagen substratum is essential for blastocyst attachment and trophoblast cell outgrowth after hatching has taken place.
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D003094:Collagen / Q000502:physiology*; D003470:Culture Media; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D051379:Mice; D011247:Pregnancy
D003470:Culture Media; D003094:Collagen