title: Synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in Veillonella, Selenomonas, and Bacteriodes species.
abstract: Evidence for reductive carboxylation of succinate to synthesize alpha-ketoglutarate was sought in anaerobic heterotrophs from the rumen and from other anaerobic habitats. Cultures were grown in media containing unlabeled energy substrates plus [14C]succinate, and synthesis of cellular glutamate with a much higher specific activity than that of cellular asparate was taken as evidence for alpha-ketoglutarate synthase activity. Our results indicate alpha-ketoglutarate synthase functions in Selenomonas ruminantium, Veillonella alcalescens, Bacteroides fragilis, Bacteroides vulgatus, Bacteroides uniformis, Bacteroides distasonis, and Bacteroides multiacidus. Evidence for this carboxylation was not found in strains representative of 10 other species.
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis; D000693:Anaerobiosis; D001439:Bacteroides / Q000378:metabolism*; D006089:Gram-Negative Anaerobic Bacteria / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000096:biosynthesis*; D007658:Ketone Oxidoreductases / Q000378:metabolism; D013045:Species Specificity; D013386:Succinates / Q000378:metabolism*; D014678:Veillonella / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D007656:Ketoglutaric Acids; D013386:Succinates; D007658:Ketone Oxidoreductases; C023096:2-oxoglutarate synthase