Modification of the Reichert FC-2 cryoattachment to improve ultrathin sectioning reproducibility.
Modifications of the Reichert FC-2 cryomicrotome attachment which facilitate sectioning of frozen material are described. Most valuable was the construction of a specimen holder chuck made of brass and connected to the specimen arm with a glass rod. Other changes include improved shielding from heat influx, a constant nitrogen pressure cooling system and a stable cryochamber mount. These modifications considerably improve reproducibility.
D000818:Animals; D005629:Frozen Sections; D008854:Microscopy, Electron; D008867:Microtomy / Q000295:instrumentation*; D010902:Pituitary Gland / Q000648:ultrastructure; D014337:Trout / Q000033:anatomy & histology