Mycotoxins in animal feedstuffs: sensitive thin layer chromatographic detection of aflatoxin, ochratoxin A, sterigmatocystin, zearalenone, and T-2 toxin.
Improvements have been made to a previously described multi-mycotoxin method that involved a membrane cleanup step. Using 2-dimensional thin layer chromatography and appropriate solvent systems, aflatoxin B1 can be detected in mixed feedstuffs and various ingredients at levels ranging from 0.1 to 0.3 microgram/kg. Corresponding detection limits for ochratoxin A and sterigmatocystin are 5 to 20 microgram/kg and for T-2 toxin and zearalenone 20 to 200 microgram/kg.
D000348:Aflatoxins / Q000032:analysis; D000821:Animal Feed / Q000032:analysis*; D002855:Chromatography, Thin Layer; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis; D013241:Sterigmatocystin / Q000032:analysis; D013605:T-2 Toxin / Q000032:analysis; D015025:Zearalenone / Q000032:analysis
D000348:Aflatoxins; D009183:Mycotoxins; D009793:Ochratoxins; D013241:Sterigmatocystin; D015025:Zearalenone; D013605:T-2 Toxin