[Influence of temperature on the preferential extraction of RNA polymerase I from hepatic nuclei of the rat].
RNA polymerase I has been extracted from rat liver nuclei by three consecutive washings at 0 degrees C with a medium of relatively low ionic strength (0.15 M KCl) containing Mg++ rather than by incubating the organelles at 37 degrees C in the same medium, as originally proposed by Chesterton and Butterworth. The modified technique, which has the advantage of preventing a temperature-mediated conversion of form IB to IA, gives similar yields of RNA polymerase I and retains the capacity of preferentially extracting the enzyme with respect to the other forms of nuclear RNA polymerase.
D000818:Animals; D002466:Cell Nucleolus / Q000201:enzymology; D002467:Cell Nucleus / Q000201:enzymology*; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification*; D008099:Liver / Q000166:cytology* / Q000201:enzymology; D012318:RNA Polymerase I / Q000302:isolation & purification*; D051381:Rats; D013696:Temperature
D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I
Influenza della temperatura sulla estrazione preferenziale di RNA poimerasi I dai nuclei epatici del ratto.