[Stabilization of dextranase from Penicillium funiculosum and Fusarium solani during heating and freeze-drying].
Freeze-drying of highly purified dextranse from Penicillium funiculosum and Fusarium solani was accompanied by 90% losses of enzyme activity and solubility. Many carbohydrates were tested as stabilizers, e.g. glucose, maltose, lactose, polyglucine, dextranase hydrolyzate of polyglucine as well as mannitol and ammonium sulfate. Polyglucine, its hydrolyzate, and glucose proved most effective stabilizers. The stabilizing effect of polyglucine hydrolyzate of dextranase during its heating and freeze-drying was compared. The effective concentration of the stabilizer during freeze-drying was 10 times lower than during heating.
D003910:Dextranase / Q000302:isolation & purification* / Q000378:metabolism; D005612:Freeze Drying; D005670:Fusarium / Q000201:enzymology*; D006358:Hot Temperature; D007700:Kinetics; D010407:Penicillium / Q000201:enzymology*
D003910:Dextranase
Stabilizatsiia preparatove dekstranazy Penicillium funiculosum i Fusarium solani pri nagrevanii i liofilizatsii.