Effect of fetal bovine serum on 3-methylcholanthrene-induced transformation of hamster cells in vitro.
Eighteen lots of fetal bovine serum were tested for their ability to support clonal growth and 3-methylcholanthrene-induced morphological transformation of hamster embryo cells in vitro. Most of them supported cloning efficiencies of over 11%. However, cloning efficiency alone was an inadequate criterion for selecting serum for transformation studies, since no transformation was observed with some lots, even though their cloning efficiencies were over 16%. This shows the importance of pretesting serum for its ability to support morphological transformation before it is used in mammalian cell carcinogenesis tests.
D000818:Animals; D002417:Cattle; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects*; D006224:Cricetinae; D046508:Culture Techniques; D004353:Drug Evaluation, Preclinical; D005312:Fetal Blood; D008168:Lung; D008748:Methylcholanthrene
D008748:Methylcholanthrene