X-ray studies of the binding of Cibacron blue F3GA to liver alcohol dehydrogenase.
The binding of Cibacron F3GA to orthorhombic crystals of liver alcohol dehydrogenase has been studied to 0.37-nm resolution. Similarities in the binding of this dye were found for rings B, C and D with the binding of the coenzyme NAD+. However, ring A of the dye and the nicotinamide ribose part of the coenzyme are quite differently bound to the enzyme.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D000873:Anthracenes / Q000378:metabolism*; D001665:Binding Sites; D004396:Coloring Agents / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008968:Molecular Conformation; D014227:Triazines / Q000378:metabolism; D014961:X-Ray Diffraction
D000873:Anthracenes; D004396:Coloring Agents; D014227:Triazines; C012842:Cibacron Blue F 3GA; D000429:Alcohol Oxidoreductases