A comparison of the in vitro metabolism of estrone, estradiol-17 beta and estrone-3-sulfate by the livers of young virgin female, pregnant and female fetal guinea-pigs.
The metabolism, in vitro, of [3H]-estrone, [3H]-estradiol-17 beta and [13H]-estrone-3-sulfate by the livers of pregnant, young virgin female and female fetus guinea-pigs has been compared using 900 g supernatants and microsomes. The ability of the guinea-pig livers to synthesize polyhydroxylated estrogens has been fo-nd to be small. The major metabolites isolated were unconjugated estrone and estradiol-17 beta or their glucuronides. The percentage of sulfates was lower after incubations with [3H]-estrone than with [3H]-estradiol-17 beta. A kinetic study with microsomes has shown a direct conversin of estrone-sulfate to estradiol sulfate. Fetal microsomes have been found to exhibit a more active hydrogenation of estrone to estradiol-17 beta than microsomes from young female or pregnant animals.
D000818:Animals; D004958:Estradiol / Q000378:metabolism*; D004970:Estrone / Q000031:analogs & derivatives* / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000378:metabolism*; D006168:Guinea Pigs; D007700:Kinetics; D008099:Liver / Q000196:embryology / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal; D013347:Subcellular Fractions / Q000378:metabolism
D004970:Estrone; D004958:Estradiol; C017296:estrone sulfate