title: Protein-protein interactions studied by counter-current distribution. I. Theoretical computations.
abstract: Many biological macromolecules are known to interact either with themselves, with other macromolecules or with small compounds. A simple equilibrium method for detecting and quantifying these interactions is to study the mutual influence of the molecules on their respective counter-current distribution in liquid-liquid biphasic systems. The theoretical counter-current distribution patterns for the components in an interacting system, A + B in equilibrium AB, have been calculated for two models in order to establish the boundary conditions and to optimize the experimental procedure. The patterns have been calculated for a range of association constants, partition coefficients and initial concentrations of the two reactants.
mesh_terms: D003377:Countercurrent Distribution; D008956:Models, Chemical; D011485:Protein Binding; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012995:Solubility
chemical_list: D011506:Proteins