[Possibilities for Ellman reaction application in the presence of detergents].
Triton X-100, triton X-305, twin-80 and sodium deoxycholate in definite concentrations lower the colour intensity of the solution which contains the product of the Ellman reaction. In the case of triton X-100 the colour disappearance is due to resynthesis of the Ellman reagent from 5-thio-2-nitrobenzoate with the presence of detergent in the concentration which is higher than the critical concentration of micelle-formation.
D000110:Acetylcholinesterase / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D003124:Colorimetry / Q000379:methods; D003840:Deoxycholic Acid; D004228:Dithionitrobenzoic Acid; D007202:Indicators and Reagents; D007700:Kinetics; D008823:Micelles; D009579:Nitrobenzoates; D011092:Polyethylene Glycols; D011136:Polysorbates
D007202:Indicators and Reagents; D008823:Micelles; D009579:Nitrobenzoates; D011136:Polysorbates; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D004228:Dithionitrobenzoic Acid; D000110:Acetylcholinesterase
Vozmozhnosti primeneniia reaktsii Ellmana v prisutstvii detergentov.