Determination of alkaline phosphatase (EC 3.1.3.1) on the SMAC using diethanolamine in a modified manifold.
The SMAC method for alkaline phosphatase was modified inorder to determine the enzyme activity according to the Scandinavian recommended method [1]. The method is not strictly 'Scandinavian', but the correlation with the strict Scandinavian method performed on the LKB 8600 Reaction rate analyzer was excellent. The modified method was linear up to at least 2000 U/l.
D000469:Alkaline Phosphatase / Q000097:blood*; D001322:Autoanalysis / Q000379:methods*; D004983:Ethanolamines; D006801:Humans; D007202:Indicators and Reagents; D012016:Reference Values; D012537:Scandinavian and Nordic Countries
D004983:Ethanolamines; D007202:Indicators and Reagents; C020283:diethanolamine; D000469:Alkaline Phosphatase