Complementation of genetic disease: a velocity sedimentation procedure for the enrichment of heterokaryons.
Methodology is described to enrich for heterokaryons after mammalian cell fusion. A heterogeneous cell mixture can be separated on a Sta-Put apparatus into fractions of uniform size cells by sedimentation through a 1% bovine serum albumin-5% Ficoll gradient. Unfused RAG and LM/TK- cells, differing by 10% in diameter, have been sorted by size; following fusion, larger and faster sedimenting cells were shown to be hybrids. This methodology can be utilized in genetic complementation studies of human genetic diseases where selection procedures for proliferating hybrids do not exist. When fibroblasts from individuals with Tay-Sachs disease [deficient in hexosaminidase A (HEX A-)] and Sandhoff-Jatzkewitz disease (HEX A- and HEX B-) are fused, HEX A is generated, demonstrating complementation of two different mutations. After Sta-Put fractionation, the HEX A complementation product was associated with the faster sedimenting multinuclear cells and not with the mononuclear parental cells. This methodology will facilitate detection of genetic differences in fibroblasts from related inherited disorders.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000378:metabolism; D004592:Electrophoresis, Starch Gel; D005816:Genetic Complementation Test; D030342:Genetic Diseases, Inborn / Q000145:classification*; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D007942:Leukemia, Experimental; D008722:Methods; D051379:Mice; D012497:Sandhoff Disease / Q000235:genetics; D013661:Tay-Sachs Disease / Q000235:genetics
D004247:DNA