[Comparative study of digoxin determination by E.L.I.S.A. and radioimmunoassay techniques (author's transl)].
This study is based on a double blind interlaboratory comparison between radioimmunoassay and E.L.I.S.A. digoxin determination. The correlation between digoxin values found with these two methods is good (r = 0.96 for therapeutic and toxic ranges 1,0 ng/ml - 5,5 ng/ml). The results indicate good repeatability, reproducibility and precision. The determination by E.L.I.S.A. can be performed with sera or plasma. The presence of haemolysis makes no appreciable difference in results. No variation is observed when different kits are used from an identical lot. However digoxin gives an important cross reactivity with digitoxin in Enzymeimmunoassay. Therefore it is necessary to know exactly the digitalis glycoside before the determination in order to avoid significant error.
D004077:Digoxin / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006461:Hemolysis; D008722:Methods; D011863:Radioimmunoassay
D004077:Digoxin
Etude comparative du dosage de la Digoxine ar méthodes Immunoenzymologique (E.L.I.S.A.) et radio-immunologique.