A simple and fast electrophoretic method for elution of nucleic acids from gels.
A very convenient electrophoretic procedure for DNA or RNA elution from agarose or polyacrylamide gels is described. The gel piece with nucleic acid to be eluted is contained in a dialysis bag filled with buffer and elution is carried out in a horizontal electrophoresis apparatus. The nucleic acid is recovered with a high yield and can be used, without prior treatment, in further enzymatic or chemical reactions. Results obtained with DNA are presented here.
D004247:DNA / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D012313:RNA / Q000302:isolation & purification*
D012313:RNA; D004247:DNA