A rapid polyethylene glycol assay for gastric intrinsic factor.
The use of polyethylene glycol (mol. wt 4000) in a rapid radioassay for intrinsic factor is reported. The assay is based on the observation that polyethylene glycol precipitates the intrinsic factor-vitamin B12 complex in the presence of serum from patients with pernicious anaemia having auto-antibodies against intrinsic factor. The assay was found highly reproducible (CV 1.8%) and well correlated to the classical method using coated charcoal in the separation step.
D000752:Anemia, Pernicious / Q000276:immunology; D001323:Autoantibodies; D005750:Gastric Juice / Q000032:analysis*; D006801:Humans; D007437:Intrinsic Factor / Q000032:analysis* / Q000276:immunology; D011092:Polyethylene Glycols; D011863:Radioimmunoassay / Q000379:methods*; D012463:Saliva / Q000032:analysis; D014805:Vitamin B 12 / Q000032:analysis
D001323:Autoantibodies; D011092:Polyethylene Glycols; D007437:Intrinsic Factor; D014805:Vitamin B 12