title: Variation of DNA polymerase activities in chick neural retina as a function of age.
abstract: The activities of DNA polymerases alpha, beta, and gamma and of thymidine kinase were determined in the chick neural retina at different stages of embryonic development (starting at seven days) and after hatching (up to five years). Crude extracts of neural retinae were fractionated by centrifugation on sucrose gradients and the enzymatic activities measured using specific assays. The DNA polymerase alpha activity decreases greatly between 7 and 11 days of incubation. This decrease parallels the decline in mitotic activity. However, a constant residual activity remains after hatching, even in the oldest animals. DNA polymerase beta activity increases slightly between 7 and 14 days of incubation; it then decreases slowly until seven days after hatching and remains constant thereafter. DNA polymerase gamma activity is maximal between 7 and 14 days of incubation and then decreases until hatching. The activity of thymidine kinase increases slightly during the embryonic life until hatching and remains almost constant thereafter. The implication of these enzymes in DNA replication and repair processes is discussed.
mesh_terms: D000375:Aging; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D004256:DNA Polymerase I / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism; D004258:DNA Polymerase III / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D012160:Retina / Q000201:enzymology* / Q000254:growth & development; D013937:Thymidine Kinase / Q000378:metabolism*
chemical_list: D013937:Thymidine Kinase; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase