The effect of retabolil and training on activity of RNA polymerase in skeletal muscles.
One hundred and twenty male albino rats weighing 180--200 g were used to determine the effect of anabolic steroid hormones on adaptive changes in the synthesis of ribosomal RNA both in sedentary animals and in animals involved in a training program. One injection of Retabolil (0.1 mg/100 g body weight) increased the alpha-amanitin insensitive RNA polymerase activity of nuclei from skeletal muscles. Fourteen h after this hormone injection the enzyme activity was 45% higher than in control animals and it remained at this level for 4 days. Under these conditions a selective binding of 19-nortestosterone with cytoplasmic proteins of skeletal muscle was found. Physical training increased the RNA polymerase activity by 50% (P less than 0.05). It was found that the testosterone binding capacity of a cytoplasmic extract from trained animals was 70% greater than that of the control animals (P less than 0.05). Four injections of Retabolil during training resulted in an additional increase of RNA polymerase activity of 40% (P less than 0.05) but reduced the testosterone binding capacity of the cytoplasmic proteins that occurred with training by 21%. The findings demonstrate the effect of anabolic hormones in the regulation of RNA synthesis in skeletal muscle nuclei in the process of their adaptation to systematic physical training.
D045930:Anabolic Agents / Q000494:pharmacology*; D000818:Animals; D003345:Corticosterone / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000201:enzymology*; D010806:Physical Education and Training; D012318:RNA Polymerase I / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D051381:Rats; D013739:Testosterone / Q000031:analogs & derivatives / Q000378:metabolism
D045930:Anabolic Agents; D012335:RNA, Ribosomal; D013739:Testosterone; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I; D003345:Corticosterone