[Detection of HBsAg and HBeAg in cleaning, rinsing and storage solutions of contact lenses and in tear fluid of carriers of HBsAg (author's transl)].
Hepatitis-B-surface antigen (HBsAg) was not detected by sensitive radioimmunoassays in the tear fluids of 6 HBsAg carriers with low and medium titers of HBsAg (less than 1:10,000) in the serum. However, HBsAg could be demonstrated in low concentrations in tear fluids of 5 of 6 HBsAg carriers with high serum titers (greater than 1:10,000). The concentration of HBsAg in the tear fluid was at least 100 times lower than in the sera of these 5 persons. Correspondingly HBsAg could be found in only 1 of the rinsing and in none of the storage solutions of the contact lenses of 7 persons with high titers of HBsAg in the serum (greater than 1:32,000). HBsAg was not adsorbed to smooth HEMA-lenses. Because of the low concentration of HBsAg in tear fluids and the dilution effect (about 5 x 10(-10)) the transmission of hepatitis B by multiple use of contact lenses by several persons during adaption is lighly unlikely. In addition, a special cleaning solution (Liprofin) can destroy nearly completely the antigenicity of HBsAg at 60 degrees C.
D000327:Adsorption; D002353:Carrier State / Q000276:immunology; D003261:Contact Lenses; D006511:Hepatitis B Antigens / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D011863:Radioimmunoassay; D012996:Solutions; D013666:Tears / Q000276:immunology*
D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens; D012996:Solutions
Zur Frage der Hepatitisübertragung durch Kontaktlinsen.