Temperature effects in affinity chromatography of alanine aminotransferase.
The effects of temperature on the elution parameters of alanine aminotransferase and albumin were studied on substituted agaroses designated for the affinity chromatography of the enzyme. The elution volume of alanine aminotransferase depended logarithmically and and the elution volume of albumin linearly on temperature. Both decreased when the temperature increased. It was concluded that the observed elution volume of alanine aminotransferase was due to two types of retardation mechanisms: specific (the logarithmic mode) and non-specific (the linear mode), both of which were additive. Thermodynamic parameters were estimated for the specific mode and the calculation resulted in delta Ho and delta So values of ca. -40 kJ/mole and -140 J/mole . degrees K, respectively.
D000410:Alanine Transaminase / Q000032:analysis*; D000418:Albumins; D002846:Chromatography, Affinity; D012685:Sepharose; D013696:Temperature; D013816:Thermodynamics
D000418:Albumins; D012685:Sepharose; D000410:Alanine Transaminase