title: [Chemical and biological properties of a fluorescence derivative of gentamycin].
abstract: By reaction with 4-phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione a fluorescing gentamycin derivative was produced which is characterized by the following properties: High fluorescence intensity in UV light, which enables simple and sensitive determination up to 10(-9) M. In this way it is possible to determine the localization of this substance in tissues, cells and body fluids. When favorably stored, the derivative is chemically stable. At pH 8 and storage at +5 degrees C in the dark for 27 days the substance loses only 5% of its original fluorescence. In comparison with the nonsubstituted gentamyzin the derivative has a twofold higher binding capacity to serum albumin. The antimicrobial properties are retained; the range of antimicrobial effects is similar to that of gentamycin.
mesh_terms: D001419:Bacteria / Q000187:drug effects; D004355:Drug Stability; D005456:Fluorescent Dyes; D005839:Gentamicins / Q000032:analysis / Q000494:pharmacology; D007700:Kinetics; D008832:Microchemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D005839:Gentamicins
vernacular_title: Chemische und biologische Eigenschaften eines Fluoreszenzderivats des Gentamyzins.