Determination of (E)-2,3-dichloro-4-methoxyphenyl)-2-furanylmethanone-O-[2-(diethylamino) ethyl]oxime methanesulphonate (ANP-4364) in plasma using gas chromatography with electron-capture detection.
A gas chromatographic method has been developed that permits the accurate and specific determination of the antianginal agent ANP-4364 in plasma. ANP-4364 is extracted with n-hexane containing ethyl chloroformate and, after a clean-up procedure, derivatized to the trichloroethyl carbamate, which is assayed on a gas chromatograph equipped with an electron-capture detector. Accurate determinations are possible over a concentration range from 1 to 50 ng/ml of ANP-4364 in plasma with a relative standard deviation of 7.5%. The minimum detectable concentration is 0.5 ng/ml. Plasma levels of ANP-4364 in dogs receiving oral (10 mg/kg) or intravenous (0.1 mg/kg) dosing have been determined.
D000787:Angina Pectoris / Q000188:drug therapy*; D000818:Animals; D002849:Chromatography, Gas; D004285:Dogs; D004583:Electrons; D005663:Furans / Q000097:blood*; D007700:Kinetics; D008297:Male; D010091:Oximes / Q000097:blood*; D012997:Solvents; D013696:Temperature
D005663:Furans; D010091:Oximes; D012997:Solvents; C010826:diclofurime