Application of 1,1'-carbonyldiimidazole-activated agarose for the purification of proteins. II. The use of an activated matrix devoid of additional charged groups for the purification of thyroid proteins.
The use of 1,1'-carbonyldiimidazole-activated agarose for biospecific affinity chromatography is described. Activation of agarose with this carbonylating reagent gives a matrix devoid of additional charged groups. Conditions for the coupling of a range of ligands and leashes have been evaluated. The efficient purification of bovine trypsin, human thyroglobulin and sheep thyroid membrane glycoproteins demonstrates the suitability of the new activated matrix for affinity chromatography.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D006023:Glycoproteins / Q000302:isolation & purification; D006801:Humans; D011506:Proteins / Q000302:isolation & purification*; D012756:Sheep; D013954:Thyroglobulin / Q000302:isolation & purification; D013961:Thyroid Gland / Q000032:analysis*; D014357:Trypsin / Q000302:isolation & purification
D006023:Glycoproteins; D011506:Proteins; D013954:Thyroglobulin; D014357:Trypsin