Assay of 3-carboxy-antipyrine in urine by capillary gas chromatography with nitrogen selective detection. Some preliminary results in man.
An assay procedure is described for 3-carboxy-antipyrine in urine using gas chromatography with a support coated open tubular capillary column (Carbowax 20M), a solid injection system and nitrogen selective detection. 3-Carboxy-antipyrine was analyzed after derivatization with diazomethane and using 4-bromo-antipyrine as internal standard. Following extraction of the urine samples with a mixture of organic solvents linear calibration curves were obtained in the concentration range of 1--50 microgram/ml. The precision was established as +/- 5.0% (n = 5), and the lowest measurable concentration was 1 microgram/ml at a signal-to-noise ratio of 10:1. Preliminary results in 5 human volunteers showed that after orl administration of 500 mg antipyrine, 3.3 +/- 0.8% of the dose was excreted as 3-carboxy-antipyrine in 52 hours' urine. The compound was excreted completely in the unconjugated form.
D000284:Administration, Oral; D000983:Antipyrine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000652:urine; D002264:Carboxylic Acids / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D008297:Male; D009584:Nitrogen
D002264:Carboxylic Acids; D009584:Nitrogen; D000983:Antipyrine