[Effect of denaturation of DNA template on activity of RNA polymerase of class B].
The effect of the denaturation of homologous and calf thymus DNA on the RNA polymerase B activity purified from rat liver and spleen and Ehrlich ascites cells, was investigated in presence of either Mn2+ or Mg2+ and in presence or absence of alpha-amanitin. On the basis of the results here reported, we suggest: 1) denatured DNA is more effective than native as template for polymerase B; 2) denatured DNA template and cations might play a role in determining the extent of the reaction alpha-amanitin-polymerase B.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D004247:DNA; D012321:DNA-Directed RNA Polymerases / Q000032:analysis*; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009691:Nucleic Acid Denaturation; D012319:RNA Polymerase II / Q000032:analysis*; D051381:Rats
D000546:Amanitins; D008345:Manganese; D004247:DNA; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium
Effetto della denaturazione del DNA template sulla attivita' della RNA polimerasi della classe B.