An efficient method to eliminate streaking in the electrophoretic analysis of haptoglobin in bloodstains.
A bloodstain extraction procedure that improves the analysis of haptoglobin in dried bloodstains has been developed. The streaking of electrophoresis gels caused by deteriorated hemoglobin can be eliminated by incorporating chloroform in the bloodstain extraction procedure. The method is easier to execute than previously published techniques for eliminating the adverse effects of deteriorated hemoglobin on the analysis of haptoglobin. Bloodstains up to two years old were correctly phenotyped in haptoglobin by this method.
D001789:Blood Group Antigens; D001801:Blood Stains; D002725:Chloroform; D004591:Electrophoresis, Polyacrylamide Gel; D006242:Haptoglobins / Q000032:analysis*; D006801:Humans; D010641:Phenotype; D013784:Textiles; D013997:Time Factors
D001789:Blood Group Antigens; D006242:Haptoglobins; D002725:Chloroform