Simple method for the simultaneous determination of noradrenaline, dopamine and serotonin by stepwise elution from a short column of weak cation-exchange resin.
A simple method for the simultaneous determination of noradrenaline, dopamine and serotonin using a short column of Amberlite CG-50 is described. Noradrenaline and dopamine were eluted from the column with phosphate buffers containing 1.5 and 4.0% boric acid, respectively, and then serotonin was eluted with 1.0 N HCl. Catecholamines were determined by a modification of the ethylenediamine condensation method using potassium ferricyanide as oxidant and isobutanol for extraction of the fluorophores. Serotonin was measured by the acidic o-phthalaldehyde method. The method was applied to the simultaneous determination of noradrenaline, dopamine and serotonin in discrete regions of rat brain.
D000818:Animals; D001923:Brain Chemistry; D002411:Cation Exchange Resins; D002852:Chromatography, Ion Exchange / Q000379:methods; D004298:Dopamine / Q000032:analysis*; D007202:Indicators and Reagents; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis*; D051381:Rats; D012701:Serotonin / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014018:Tissue Distribution
D002411:Cation Exchange Resins; D007202:Indicators and Reagents; D009419:Nerve Tissue Proteins; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine