Local anesthetics block induction of the Pseudomonas alk regulon.
The local anesthetics procaine and piperocaine blocked induction of the plasmid-determined enzymatic activities involved in the metabolism of n-alkanes in Pseudomonas putida. Procaine reversibly inhibited existing alkane hydroxylase activity. Induction of a soluble aliphatic amidase activity was not affected. These results support the hypothesis that induction of the plasmid-determined alkane metabolic system in P. putida involves a membrane component(s).
D000473:Alkanes / Q000378:metabolism; D000581:Amidohydrolases / Q000096:biosynthesis; D000779:Anesthetics, Local / Q000494:pharmacology*; D001565:Benzoates / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D010880:Piperidines / Q000494:pharmacology*; D010957:Plasmids; D011343:Procaine / Q000494:pharmacology*; D011549:Pseudomonas / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D000473:Alkanes; D000779:Anesthetics, Local; D001565:Benzoates; D010880:Piperidines; D011343:Procaine; D006899:Mixed Function Oxygenases; D000581:Amidohydrolases; C023039:piperocaine