Determination of L-alanosine in plasma and urine by reversed-phase high-performance liquid chromatography of the Dns derivative.
L-Alanosine is an antitumour antibiotic that has recently been placed in clinical trial. We have developed a relatively rapid and specific assay for urinary and plasma alanosine, based on formation of the Dns derivative and separation of this from other Dns compounds by reversed-phase high-performance liquid chromatography. Dns-Alanosine is detected by its absorption at 254 nm, since alanosine is atypical in that it forms a Dns derivative with very low fluorescence. The lower limit of detection of alanosine in plasma is 0.1 microgram/ml. The assay has been used to measure the levels of alanosine in the plasma and urine of rabbits and of man.
D000409:Alanine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds; D005260:Female; D006801:Humans; D008297:Male; D008832:Microchemistry; D009602:Nitrosamines / Q000032:analysis / Q000097:blood / Q000652:urine; D011817:Rabbits
D000903:Antibiotics, Antineoplastic; D003619:Dansyl Compounds; D009602:Nitrosamines; C000082:alanosine; D000409:Alanine