A basic program to correct for split cell error in particles classified by the logarithm of their profile areas.
A program is presented, written in the BASIC computer language, to correct the errors which occur when cell profiles are counted in thin sections of tissue. Given the numbers of profiles in each of M size classes, and the thickness and area of the sections examined, the program calculates the number of cells in each of these classes per mm3 of tissue. The mathematical method is briefly described and it is shown that a useful level of accuracy may be obtained when the correction procedure is used in the study of non-spherical cells.
D002452:Cell Count / Q000379:methods*; D003201:Computers; D008433:Mathematics