Autoradiographic studies on the incorporation of tritiated uridine, leucine and methionine into Gomori-positive glia of rat hypothalamus.
The relative level of metabolic activity in Gomori-positive (Gp) glia was estimated by comparing the incorporation rates of 3H-uridine, 3H-leucine and 3H-methionine into the nuclear macromolecules of the glia, neurons, ependyma and subcommissural organ (SCO). The uptake of 3H-uridine by Gp glia was somewhat lower than that by neurons and SCO but higher than that by ependyma. The incorporation of 3H-leucine was about the same in all studied types of cells, except for a lower uptake by ependyma. The rate of 3H-methionine incorporation into Gp glia and SCO was higher than that into neurons and ependyma. The neuron: Gp glia ratios obtained in the present study (0.9--1.4) are more or less within the range of the neuron: cerebral glia ratios reported by others for newly synthesized molecules of RNA and proteins. The results suggest that Gp glia have a relatively high metabolic activity as compared to that in neurons and perhaps SCO.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000166:cytology; D002467:Cell Nucleus / Q000378:metabolism; D007031:Hypothalamus / Q000166:cytology*; D007930:Leucine / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D014316:Tritium; D014529:Uridine / Q000378:metabolism*
D014316:Tritium; D008715:Methionine; D007930:Leucine; D014529:Uridine