title: The effect of long-term arterial occlusion on energy metabolism of the human quadriceps muscle.
abstract: The effect of 1.5 to 2.5 h tourniquet ischemia on energy metabolism of the quadriceps muscle was studied using percutaneous needle biopsy technique in sixteen patients operated on for an inveterated knee injury. During occlusion there was a moderate decrease in ATP with an increase in ADP and AMP. This change resulted in a decreased energy charge potential. At the same time phosphorylcreatine (PC) decreased markedly while creatine (Cr) increased giving a constant total creatine (TCr). An accumulation of lactate during occlusion with values up to 80 mmol/kg d.m. (dry muscle) was seen. A 15% reduction in glycogen was calculated. After release of the tourniquet the active phosphate concentration and the energy charge potential returned to basal levels within 5 min and most of the metabolites in the glycolytic sequence were also normalized. Muscle lactate content was normal after 30 min of intact circulation. The results suggest that longterm tourniquet ischemia induces marked changes in energy metabolism in skeletal muscle, but that the changes are rapidly and completely reversible with restoration of blood flow.
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D001158:Arteries / Q000502:physiology; D003401:Creatine / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D006801:Humans; D007511:Ischemia / Q000378:metabolism* / Q000503:physiopathology; D007718:Knee Injuries / Q000378:metabolism; D007773:Lactates / Q000097:blood; D008023:Ligaments, Articular / Q000293:injuries; D008297:Male; D009132:Muscles / Q000098:blood supply / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D013997:Time Factors; D014111:Tourniquets
chemical_list: D007773:Lactates; D010710:Phosphates; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003401:Creatine