The separation of nucleic acids and related compounds on nucleobase-coupled cellulose.
The affinity chromatography on uracil-coupled cellulose was carried out for the separation of nucleosides, nucleotides and oligonucleotides. Adenine derivatives exhibited a high affinity to uracil-cellulose, and sequencial isomers of oligonucleotides containing adenine residue were resolved. Poly(A) was strongly bound to uracil-cellulose and recovered by the elution with 7M urea. This procedure was extended to the isolation of mRNA containing poly(A) sequences.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D008099:Liver / Q000032:analysis; D051379:Mice; D009705:Nucleosides / Q000302:isolation & purification*; D009711:Nucleotides / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000302:isolation & purification*; D011119:Polynucleotides / Q000302:isolation & purification*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D012335:RNA, Ribosomal / Q000302:isolation & purification; D012156:Reticulocytes / Q000378:metabolism
D009705:Nucleosides; D009711:Nucleotides; D009841:Oligonucleotides; D011119:Polynucleotides; D012333:RNA, Messenger; D012335:RNA, Ribosomal