Glass capillary gas chromatography with electron-capture detection. Separation of prostaglandins.
Glass capillary gas chromatography of the prostaglandins was performed on a system including an all-glass, solventless injector; thermostable methylphenyl-polysiloxane glass capillary columns; and a conventional electron-capture detector fitted with a make-up gas tee. The principal stable metabolites of prostaglandin endoperoxide were separated as perfluorinated derivatives in 35 min. Detection limits equal or exceed those obtained for packed column separations and electron capture detection. Prostaglandin endoperoxide metabolic profiles from mammalian cell cultures were obtained using this system. These profiling studies are not possible with other chromatographic methods because of inferior resolution and sensitivity.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002849:Chromatography, Gas / Q000379:methods; D008214:Lymphocytes / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D011453:Prostaglandins / Q000302:isolation & purification*; D011463:Prostaglandins H / Q000378:metabolism; D013154:Spleen / Q000032:analysis; D013950:Thymus Gland / Q000032:analysis
D011453:Prostaglandins; D011463:Prostaglandins H