Trace analysis of the MIF analogue pareptide in blood plasma by high-performance liquid chromatography and short-wavelength excitation fluorometry.
A high-performance liquid chromatographic procedure was developed and applied to analysis of the pharmacologically active MIF analogue pareptide in human plasma. The procedure involves formation of a fluorescent 7-chloro-4-nitrobenzyl-2-oxa-1,3-diazole (NBD-Cl) pareptide derivative followed by separation of the NBD derivative from plasma components on a 30-cm microparticle octadecylsilane bonded column. The separated derivative was quantitated using a short-wavelength excitation fluorometric detector. The detection limit of pareptide in plasma samples was 5 ng or 17 pmoles per ml of plasma. In the absence of plasma, the corresponding on-column detection limit was 0.5 pmoles.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009075:MSH Release-Inhibiting Hormone / Q000031:analogs & derivatives*; D009842:Oligopeptides / Q000097:blood*; D013050:Spectrometry, Fluorescence / Q000379:methods
D009842:Oligopeptides; C024569:pareptide; D009075:MSH Release-Inhibiting Hormone