Antigen-antibody complexes stimulate the synthesis and release of prostaglandins by mouse peritoneal macrophages.
Antigen-antibody complexes (Ag/Ab) formed at equivalence stimulate the release of arachidonic acid and synthesis of prostaglandin E2 and 6-keto-prostaglandin F1 alpha by resident mouse peritoneal macrophages. Prostaglandin synthesis and secretion is stimulated by submicrogram quantities of Ag/Ab which increases in a dose-dependent manner. This release is time-dependent and occurs in the absence of any loss of cell viability as indicated by increased cellular levels of lactate dehydrogenase without concomitant loss of this activity to the media and the continued secretion of a constitutive cellular product, lysozyme. The stimulated synthesis of prostaglandins by Ag/Ab is inhibited by indomethacin and physiological levels of antiinflammatory glucocorticoids.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000936:Antigen-Antibody Complex; D001095:Arachidonic Acids / Q000378:metabolism; D001202:Ascitic Fluid / Q000166:cytology; D046508:Culture Techniques; D007213:Indomethacin / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D009113:Muramidase / Q000378:metabolism; D011448:Prostaglandin Antagonists; D011458:Prostaglandins E / Q000096:biosynthesis* / Q000378:metabolism; D011460:Prostaglandins F / Q000096:biosynthesis* / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D000936:Antigen-Antibody Complex; D001095:Arachidonic Acids; D011448:Prostaglandin Antagonists; D011458:Prostaglandins E; D011460:Prostaglandins F; D007770:L-Lactate Dehydrogenase; D009113:Muramidase; D007213:Indomethacin