Thermogenic responses of brown adipocytes to noradrenaline and glucagon in heat-acclimated and cold-acclimated rats.
Heat production of isolated brown-fat cells by addition of noradrenaline and glucagon was measured in warm-acclimated control, cold-acclimated and heat-acclimated rats by use of a twin-type conduction microcalorimeter. Noradrenaline and glucagon induced maximum heat production per 10(6) cells in dose of 1 microgram/ml. Heat produced by maximum thermogenic response to glucagon was twice as much as that to noradrenaline. Thermogenic response to noradrenaline was markedly increased in cold-acclimated brown adipocytes, while it was reduced in heat-acclimated ones. Thermogenic response to glucagon was significantly reduced in heat-acclimated brown adipocytes, while it was not affected in cold-acclimated brown adipocytes.
D000064:Acclimatization; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects*; D002001:Adipose Tissue, Brown / Q000187:drug effects*; D000818:Animals; D001833:Body Temperature Regulation / Q000187:drug effects*; D002151:Calorimetry; D003080:Cold Temperature; D004305:Dose-Response Relationship, Drug; D005934:Glucagon / Q000494:pharmacology*; D006358:Hot Temperature; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats
D005934:Glucagon; D009638:Norepinephrine