Turnover of circulating hematopoietic stem cells.
Short-term parabiosis of male and female CBA/CaJ mice was used to investigate the turnover of circulating hematopoietic stem cells. The exchange and subsequent disappearance of donor stem cells were monitored by spleen colony assay and chromosome analysis of individual colonies. The results revealed an exponential disappearance of pluripotent stem cells from blood with a characteristic half time of 1.7 h. Blood-borne stem cells were shown to be equilibrated with a subpopulation of marrow stem cells exhibiting a disappearance half time of 9.5 h. Splenectomy did not change the apparent rate of stem cell removal from the blood.
D000818:Animals; D001854:Bone Marrow Cells; D002452:Cell Count; D002453:Cell Cycle; D002875:Chromosomes; D003114:Colony-Forming Units Assay; D005070:Evans Blue; D005260:Female; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010227:Parabiosis; D013154:Spleen / Q000166:cytology; D013156:Splenectomy
D005070:Evans Blue