[Identification of 4-O-methyldopamine in rat tissues by reverded-phase liquid chromatography (author's transl)].
4-O-Methyldopamine was identified and assayed in tissues from L-dopa treated rats by reversed-phase high-performance liquid chromatography. The initial steps in the separation of catecholamines were performed by alumine, a weak cation-exchange resin, and thin-layer chromatographic techniques. After L-[3 H] dopa administration, the radiochromatogram was superimposed on the fluorochromatogram obtained with authentic marker 4-O-methyldopamine. This metabolite was detected in kidney but not in brain. The 4-O-methyldopamine:3-O-methyldopamine ratio was 0.032 in kidney. The influence of various treatments on this ratio was investigated. A 160% increase was found after L-dopa administration. This effect was potentiated by nialamide pretreatment (550% increase).
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003846:Deoxyepinephrine / Q000032:analysis*; D004298:Dopamine / Q000031:analogs & derivatives*; D007668:Kidney / Q000032:analysis; D007980:Levodopa / Q000494:pharmacology; D008297:Male; D051381:Rats
D007980:Levodopa; D003846:Deoxyepinephrine; D004298:Dopamine
Identification de la 4-O-méthyl dopamine dans les tissus de rat par chromatographie liquide en phase inverse.