Characterization of two Salmonella newport bacteriophages.
Salmonella newport phages 16--19 and 7--11 have very long heads and are members of two rare and so far little-known phage groups. Both produce various morphological aberrations. Preparations of phage 7--11 contain numerous polyheads and about 0.4% short heads belonging to nine size classes. In addition, one giant phage particle was observed. The head of phage 7--11 seems to be an icosahedron which became elongated by adding successive rows of subunits. Phages 16--19 and 7--11 have buoyant densities in CsCl of 1.43 and 1.48 g/mL and particle weights of 103 and 204 x 10(6) respectively. Both viruses contain double-stranded DNA, internal proteins, and sugars. Phage 16--19 contains 46.5% DNA of 35 x 10(6) molecular weight, and glucose. Phage 7--11 contains 47.5% DNA of 108 x 10(6) molecular weight, and mannose. Base compositions of phage and S. newport DNAs were determined from buoyant densities, melting point, and acid hydrolysis. Phage 16--19 contains 5.4% 5-methylcytosine.
D001482:Base Composition; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006147:Guanine / Q000032:analysis; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D012475:Salmonella / Q000032:analysis; D012483:Salmonella Phages / Q000648:ultrastructure*; D014764:Viral Proteins / Q000032:analysis
D004269:DNA, Bacterial; D004279:DNA, Viral; D014764:Viral Proteins; D006147:Guanine; D003596:Cytosine; D005947:Glucose; D008358:Mannose