Conversion of 7-ketolithocholic acid to ursodeoxycholic acid by human intestinal anaerobic microorganisms: interchangeability of chenodeoxycholic acid and ursodeoxycholic acid.
Chenodeoxycholic acid, ursodeoxycholic acid and 7-ketolithocholic acid were incubated with human intestinal bacteria (source: 4 healthy males) at 37 degrees C for 72 hours in an anerobic condition. The bile acids of the products in culture medium were identified by three independent methods, thin layer chromatography, gas-liquid chromatography and GLC-mass spectrometry. Lithocholic acid, ursodeoxycholic acid and 7-ketolithocholic acid were observed in the culture of chenodeoxycholic acid. Lithocholic acid, chenodeoxycholic acid and 7-ketolithocholic acid were observed in the culture of ursodeoxycholic acid. Chenodeoxycholic acid and ursodeoxycholic acid were produced from 7-ketolithocholic acid. These data may suggest that chenodeoxycholic acid and ursodeoxycholic acid are interconvertible via 7-ketolithocholic acid by the mixed culture of human intestinal microorganisms under an anaerobic condition.
D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism*; D002635:Chenodeoxycholic Acid / Q000378:metabolism*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003840:Deoxycholic Acid / Q000031:analogs & derivatives*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008095:Lithocholic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D014580:Ursodeoxycholic Acid / Q000378:metabolism*
D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; C023020:7-ketolithocholic acid; D008095:Lithocholic Acid; D014580:Ursodeoxycholic Acid