High-speed steric exclusion chromatography of biopolymers.
Biopolymer separations were studied on Micropak TSK type SW columns, which contain an aqueous compatible steric exclusion support. Columns of two different pore sizes, designated 2000SW and 3000SW, were compared for separation of proteins and nucleic acids covering a molecular weight range of 13,500 to 340,000 and the effect of molecular shape and denaturation upon elution volume was investigated. Use of high-speed steric exclusion chromatography as a prefractionation step prior to ion-exchange chromatography in biopolymer purification schemes is discussed.
D000818:Animals; D001704:Biopolymers; D001798:Blood Proteins / Q000302:isolation & purification*; D002850:Chromatography, Gel / Q000379:methods*; D006801:Humans; D008099:Liver / Q000032:analysis; D008970:Molecular Weight; D009696:Nucleic Acids / Q000302:isolation & purification*; D012343:RNA, Transfer / Q000032:analysis; D011817:Rabbits
D001704:Biopolymers; D001798:Blood Proteins; D009696:Nucleic Acids; D012343:RNA, Transfer