Effect of pyridoxine, glyoxylic acid and pyridoxylate on oxidative metabolism in vitro and phosphorylated energy-rich compounds studied in rat brains during acute hypoxia and ischaemia.
Pyridoxine (1-8 mmol/l) did not change significantly the cerebral oxygen nor the hypoxic or ischaemic degradation of phosphocreatine and ATP. Glyoxylic acid (1-8 mmol/l), an inhibitor of the citric acid cycle, depressed the electrically stimulated oxygen uptake of brain slices to a lesser extent than did pyridoxylate. Moreover, at concentrations of 0.66 mmol/l, pyridoxylate predominantly delayed the hypoxic or the ischaemic breakdown of creatine phosphate and of ATP compared with glyoxylic acid (0.66 mmol/l). These findings paralleled clearly the prominent hypoxic and post-hypoxic protection afforded by pyridoxylate upon rat brain electrogenesis, reported in the preceding paper.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D004558:Electric Stimulation; D006038:Glyoxylates / Q000494:pharmacology*; D002534:Hypoxia, Brain / Q000378:metabolism*; D007539:Isonicotinic Acids / Q000494:pharmacology*; D007773:Lactates / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D011735:Pyridoxic Acid / Q000494:pharmacology*; D011736:Pyridoxine / Q000494:pharmacology*; D051381:Rats
D006038:Glyoxylates; D007539:Isonicotinic Acids; D007773:Lactates; D010710:Phosphates; D010725:Phosphocreatine; D011735:Pyridoxic Acid; D000255:Adenosine Triphosphate; D011736:Pyridoxine; D010100:Oxygen