Identification and quantitation of nucleosides, bases and other UV-absorbing compounds in serum, using reversed-phase high-performance liquid chromatography. II. Evaluation of human sera.
The reversed-phase mode of high-performance liquid chromatography was used to investigate the profiles of low-molecular-weight, UV-absorbing compounds in human serum. Identification techniques are described which allow for the identification of picomole amounts of the nucleosides, bases and other compounds in several microliters of serum ultrafiltrate. The sera from 31 normal subjects (17 males, 14 females) showed very consistent profiles. A total of 12 compounds were identified and quantified in normal serum. The analysis of sera from over 150 patients with various types of neoplasia and other diseases showed serum profiles significantly different from normal profile.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000097:blood; D009705:Nucleosides / Q000097:blood*; D011687:Purines / Q000097:blood; D011743:Pyrimidines / Q000097:blood
D009705:Nucleosides; D011687:Purines; D011743:Pyrimidines