The fluorescence and bright field microscopic demonstration of cathepsin B in human fibroblasts.
Cathepsin B was demonstrated cytochemically in human fibroblasts with Z-Ala-Arg-Arg-2-(4-methoxy)naphtylamide as substrate. The enzyme was visualized in the bright field microscope with the diazonium salt Fast Blue B as coupling reagent and in the fluorescence microscope with 5-nitrosalicylaldehyde. With both methods cathepsin B was found in small granules distributed throughout the cytoplasm.
D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D002403:Cathepsins / Q000032:analysis*; D005347:Fibroblasts / Q000201:enzymology; D006651:Histocytochemistry; D006801:Humans; D008856:Microscopy, Fluorescence
D002403:Cathepsins