Methylthiohydantoin amino acids: chromatographic separation and comparison to phenylthiohydantoin amino acids.
Most phenylthiohydantoin (PTH) amino acids and most methylthiohydantoin (MTH) amino acids may be separated from one another by thin-layer chromatography (TLC) using the same sequential development technique with the same two solvents. Similarly, a single solvent system may be used in high-performance liquid chromatography (HPLC) to separate most PTH-amino acids and most MTH-amino acids. When both TLC and HPLC separations are performed on a sample, all MTH-and PTH-amino acids can be uniquely identified. Since many solid-phase protein sequencing techniques generate both MTH-and PTH-amino acids, these analytical systems simplify identification of the amino acid derivatives. Although the chromatographic properties of MTH-and PTH-amino acids are similar, they are not identical (contrary to a previous report).
D000596:Amino Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D006827:Hydantoins; D007202:Indicators and Reagents; D010455:Peptides; D010669:Phenylthiohydantoin; D011506:Proteins; D013867:Thiohydantoins
D000596:Amino Acids; D006827:Hydantoins; D007202:Indicators and Reagents; D010455:Peptides; D011506:Proteins; D013867:Thiohydantoins; D010669:Phenylthiohydantoin; C023447:methylthiohydantoin