Cation requirement of natural, in-vitro generated and antibody dependent killing exerted by human lymphocytes.
Three cytotoxic systems, NK, ADCC and the seemingly indiscriminative cytotoxicity of in vitro activated lymphocytes (cultivated with K 562) were found to require Ca++ and did not function in Mg++. The optimum concentration of Ca++ was identical for the three systems and for various lymphocyte subsets. 15 minutes after initiation of the interaction cell damage had already occurred in all three systems.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002118:Calcium / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008274:Magnesium / Q000276:immunology*; D011817:Rabbits
D008274:Magnesium; D002118:Calcium