title: Characterization of ferritin from human placenta. Implications for analysis of tissue specificity and microheterogeneity of ferritins.
abstract: Mammalian ferritins can be resolved into multiple components by isoelectric focusing, and each tissue contains a characteristic subset of isoferritins. Ferritin isolated from human liver was compared to acidic ferritin isolated from mid-gestational human placenta to define a structural basis for ferritin heterogeneity. Placenta ferritin contained several major bands with isoelectric points in the range of pI = 4.7-5.0 which were more acidic than the predominant isoferritins of human liver. Ferritin from each tissue was resistant to denaturation by 10 M urea and appeared to be identical by electron microscopy. Circular dichroism measurements revealed that placenta ferritin had substantially less ordered secondary structure than liver ferritin. Both types of ferritin contained only two subunits when analyzed by electrophoresis in sodium dodecyl sulfate gels, but isoelectric focusing of dissociated subunits in urea revealed 6-7 different components. In this system, placenta ferritin was enriched in the more acidic subunits and it completely lacked the most basic subunits noted in liver ferritin; placental ferritin had no unique components. Differences in isoelectric points among assembled ferritins from these two tissues appear to result from different proportions of these acidic and basic subunits.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D005293:Ferritins / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D008099:Liver / Q000032:analysis; D009928:Organ Specificity; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy
chemical_list: D005293:Ferritins