Amino acid sequence differences in pancreatic ribonucleases from water buffalo breeds from Indonesia and Italy.
The amino acid sequences of the pancreatic ribonucleases from river-breed water buffaloes from Italy and swamp-breed water buffaloes from Indonesia differ at three positions. One of the differences involves a replacement of asparagine-34, with covalently attached carbohydrate on all molecules, in the river-breed enzyme by serine in the swamp-breed enzyme. The ribonuclease content of the pancreas differs considerably between breeds and is lower in river buffaloes. A ribonuclease preparation from two swamp buffaloes contained a minor glycosylated component. Preliminary evidence was obtained that the amino acid sequence of this component has factors in common with the main component of the swamp-breed ribonuclease and with the river-breed enzyme.
D000595:Amino Acid Sequence; D000818:Animals; D002020:Buffaloes / Q000378:metabolism*; D007214:Indonesia; D007558:Italy; D010179:Pancreas / Q000201:enzymology*; D012260:Ribonucleases / Q000032:analysis*; D013045:Species Specificity
D012260:Ribonucleases