Native and reconstituted chromosome fiber fragments.
The structure of hen erythrocyte chromatin fibers was studied with the electron microscope. Chromatin fiber fragments with a length of about 5,000 A and an average diameter of 320 A are composed of 13 globular subunits (superbeads) which contain different numbers of nucleosomes. Their number average corresponds to 17 nucleosomes. - The interaction of lysine-rich histones with nucleosome chains was investigated by reconstitution experiments and was found to be semi-cooperative.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000378:metabolism; D004912:Erythrocytes / Q000648:ultrastructure; D005260:Female; D006657:Histones / Q000378:metabolism; D008836:Micrococcal Nuclease / Q000378:metabolism; D009707:Nucleosomes / Q000648:ultrastructure*; D011485:Protein Binding
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D004247:DNA; D008836:Micrococcal Nuclease