The chromatographic separation of factor VIII on aminohexyl sepharose.
A new method of factor VIII purification has been devised which involves chromatographic separation on aminohexyl-substituted agarose. Relatively large volumes of starting material can be processed compared to the volume of agarose employed and satisfactory yields are obtained. Factor-VIII-clotting activity is separated from the other related substances and resultant products appear to be stable. While separation of clotting activity occurs with relative ease, the antigen and ristocetin co-factor are hardly segregated, if at all. This provides a little more information about the interrelation of these substances. Results suggest that ion-exchange is involved in the mechanism of separation but additional hydrophobic or steric effects cannot be ruled out.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D002847:Chromatography, Agarose; D005169:Factor VIII / Q000302:isolation & purification*; D006801:Humans; D008722:Methods; D013552:Swine
D000941:Antigens; D005169:Factor VIII