title: Phagocytosis and killing of Trypanosoma dionisii by human neutrophils, eosinophils and monocytes.
abstract: The cell-mediated resistance of human leucocytes to Trypanosoma dionisii, a bat parasite related to T. cruzi, was investigated. Human peripheral blood neutrophils and monocytes were cytotoxic to T. dionisii as assessed by electron microscopy and by induction of 99mTc release from trypanosomes pre-labelled with [99mTc] pertechnetate. The enhancement of cytotoxicity by specific antiserum varied considerably from one individual to another. Neither blood lymphocytes nor blood eosinophils induced 99mTc release from T. dionisii. The trypanosomes were readily phagocytosed by neutrophils and monocytes even in the absence of added antiserum but the rate was enchanced when antiserum was present. Eosinophils also phagocytosed T. dionisii but only in the presence of antiserum. Investigation by electron microscopy revealed that T. dionisii is rapidly destroyed in the phagocytic vacuole of enutrophils and monocytes and by eosinophils. Phagocytosis, ultrastructural damage and induction of 99mTc release occurred more rapidly in neutrophils than in monocytes.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002685:Chiroptera / Q000382:microbiology; D003602:Cytotoxicity, Immunologic; D004804:Eosinophils / Q000276:immunology*; D006801:Humans; D008854:Microscopy, Electron; D009000:Monocytes / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis; D013667:Technetium; D014345:Trypanosoma / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D013667:Technetium