title: Mercapturic acid occurrence in fish bile. A terminal product of metabolism of the herbicide molinate.
abstract: 1. Molinate sulphoxide, an oxidation metabolite of molinate, is cleaved in vitro by Japanese carp liver cytosol fraction, indicating the presence of GSH-S-transferase activity, since cleavage of the sulphoxide is dependent on the amount of supernatant protein and GSH in the assay medium. 2. Molinate mercapturate was identified as a major metabolite recovered from bile of carp treated with molinate. 3. The structure of the mercapturate was established by co-chromatography with an authentic standard in three t.l.c. systems and by mass spectrometry.
mesh_terms: D000111:Acetylcysteine / Q000378:metabolism*; D000818:Animals; D001381:Azepines / Q000378:metabolism*; D001646:Bile / Q000378:metabolism*; D001711:Biotransformation; D002219:Carbamates; D002347:Carps / Q000378:metabolism*; D003530:Cyprinidae / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006540:Herbicides / Q000378:metabolism*; D010084:Oxidation-Reduction; D013454:Sulfoxides / Q000378:metabolism; D013859:Thiocarbamates
chemical_list: D001381:Azepines; D002219:Carbamates; D006540:Herbicides; D013454:Sulfoxides; D013859:Thiocarbamates; C009821:molinate; D005978:Glutathione; D000111:Acetylcysteine