Evidence that the fetal mouse liver cell assay detects erythroid regulatory factors (ERF) not measured with a standard in vivo assay.
A simplified modification of the fetal mouse liver cell (FMLC) assay has been used to investigate the relationship between in vivo and in vitro detectable Erythroid Regulatory Factors (ERF) in the serum of dogs exposed to hypoxia. The results suggest that the FMLC assay is a useful technique for following changes in serum ERF. However, the data obtained cannot be directly equated with the in vivo erythrocythemic mouse assay unless a correction factor is applied. This is necessary because the in vitro assay, in general, detects 50% more ERF than does the standard mouse assay.
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D004285:Dogs; D004921:Erythropoietin / Q000097:blood*; D005260:Female; D005333:Fetus; D000860:Hypoxia / Q000097:blood; D008099:Liver / Q000166:cytology; D051379:Mice
D004921:Erythropoietin