title: Effect of pentobarbital and urethane on the release of hypothalamic somatostatin and pituitary growth hormone.
abstract: Hypothalamic somatostatin release was investigated in the rat to elucidate the mechanism of anesthetic action on growth hormone (GH) release from the pituitary. Intraperitoneal injection of sodium pentobarbital (5 mg/100 gm B.W.) significantly elevated serum GH levels and increased hypothalamic somatostatin concentration from basal values of 0.98 +/- 0.01 to 1.21 +/- 0.06 ng/mg wet wt. In contrast, urethane (150 mg/100 gm B.W., IP) administration lowered serum GH levels and hypothalamic somatostatin concentration (0.64 +/- 0.04 ng/mg wet wt.). However, the mean concentration of pancreatic somatostatin showed no change in either case. In rats receiving passive immunization with 0.5 ml rabbit antiserum to somatostatin (SRIF-AS), serum GH levels were significantly increased (67.5 +/- 12.3 ng/ml) and did not differ from those in the group treated with normal rabbit serum (NRS) plus pentobarbital (101.3 +/- 18.5 ng/ml). However, serum GH levels in rats injected with SRIF-AS plus pentobarbital were increased to higher values than in rats given SRIF-AS alone. When urethane was administered to rats after passive immunization with SRIF-AS, urethane-induced suppression of serum GH levels was markedly inhibited (5.5 +/- 2.0 vs. 33.5 +/- 7.5 ng/ml). These results suggest a possibility that the changes in serum GH levels observed with pentobarbital or urethane administration may be induced at least in one part by somatostatin released from the hypothalamus.
mesh_terms: D000818:Animals; D013006:Growth Hormone / Q000097:blood*; D007031:Hypothalamus / Q000378:metabolism*; D007116:Immunization, Passive; D008297:Male; D010179:Pancreas / Q000378:metabolism; D010424:Pentobarbital / Q000494:pharmacology*; D051381:Rats; D013004:Somatostatin / Q000097:blood* / Q000276:immunology; D014520:Urethane / Q000494:pharmacology*
chemical_list: D014520:Urethane; D013004:Somatostatin; D013006:Growth Hormone; D010424:Pentobarbital