Class separation of bile lipids by thin-layer chromatography.
A thin-layer chromatography technique is described that permits separation of each class of bile lipid, such as cholesterol, free (unconjugated) bile acids, glycine- and taurine-conjugated bile acids and phospholipids, in a single run. The use of silica gel G-aluminium pre-coated sheets facilitates further processing, such as the extraction in situ of each class of separated bile lipids for determination by conventional methods.
D001646:Bile / Q000032:analysis*; D001647:Bile Acids and Salts / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer / Q000379:methods; D005704:Gallbladder / Q000032:analysis; D005998:Glycine / Q000302:isolation & purification; D006801:Humans; D010743:Phospholipids / Q000302:isolation & purification*; D013261:Sterols / Q000302:isolation & purification*; D013654:Taurine / Q000302:isolation & purification
D001647:Bile Acids and Salts; D010743:Phospholipids; D013261:Sterols; D013654:Taurine; D005998:Glycine