[Production of primary cell cultures on a semi-industrial scale. 1. Modification of the trypsinization procedure--possible means to improve cell yield].
To produce large quantities of primary cell suspensions on efficient technique is described which is named "Biomix-Method". By this technique first tissue mince is performed automatically using tissue amounts between 300 g and 500 g. Trypsinization takes place in a three-litre trypsinization vessel using a stirring mechanism according to a defined trypsinization mode. In regard to optimal cell seeding concentration and a justifiable productivity one kidney of newborn or suckling calf yields 6--9 1 cell suspension and that of piglet 1,8-2,3 1, respectively. Proliferation behaviour and effectivity of primary cells are discussed and compared with other cell systems.
D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D002417:Cattle; D002455:Cell Division; D002642:Chick Embryo; D003470:Culture Media; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D005347:Fibroblasts; D007668:Kidney; D013552:Swine; D014357:Trypsin
D003470:Culture Media; D014357:Trypsin
Zur Herstellung von Frischzellkulturen im halbindustriellen Mass-stab. 1. Mitteilung: Uber eine Modifikation des Trypsinationsprozesses--eine Möglichkeit zur Verbesserung der Zellausbeute.