The binding of 8-anilinonaphthalene-1-sulphonate to a fixed concentration of unenergised and succinate-energised submitochondrial particles.
The effect of the approximately hyperbolic relationship between fluorochrome concentration and light absorbed on the interpretation of data for the binding of 8-anilinonaphthalene-1-sulphonate to unenergised and succinate-energised submitochondrial particles has been investigated. If this is taken into account plots of fluorescence against fluorescence x [fluorochrome]-1 do not tend towards a maximum fluorescence value. The significance of this findings is discussed.
D000817:Anilino Naphthalenesulfonates; D000818:Animals; D002417:Cattle; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013367:Submitochondrial Particles / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism*
D000817:Anilino Naphthalenesulfonates; D013386:Succinates