Inhibition by trioxalen (psoralen) plus near-ultraviolet light of the induction of ornithine decarboxylase in Chinese-hamster cells.
Trioxalen (trimethylpsoralen) plus near-u.v. light, a potent inhibitor of DNA and RNA synthesis, inhibits the induction of ornithine decarboxylase in stationary-phase V79 fibroblasts. It does not affect the translation of pre-existing mRNA. The method, in view of its high degree of specificity and precise timing, is a better choice for inhibiting RNA synthesis than the commonly used chemical inhibitors and precursor analogues.
D000818:Animals; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004790:Enzyme Induction / Q000187:drug effects / Q000528:radiation effects; D005347:Fibroblasts / Q000201:enzymology; D011564:Furocoumarins / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D014307:Trioxsalen / Q000494:pharmacology*; D014466:Ultraviolet Rays
D011564:Furocoumarins; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D014307:Trioxsalen