Anthramycin-induced sister-chromatid exchange and caffeine potentiation in the chromosomes of Indian muntjac.
Cell-cycle kinetics, sister-chromatid exchange (SCE) and chromosome aberrations have been studied from the skin fibroblasts of the Indian muntjac after treatment with 100 micrograms/ml of caffeine and 0.05 microgram/ml of anthramycin. The cultures were incubated for a period which was sufficient for the completion of two consecutive cell cycles and both the drugs appeared to produce a slight inhibitory effect. When anthramycin-treated cells were however post-treated with caffeine, the cells did not proceed beyond one cycle and exhibited a mitotic block. The SCE frequency in the control and the experiments with caffeine and anthramycin was 8.63, 18.32 and 34.88 per cell respectively. The SCEs were randomly distributed amongst all chromosomes unlike a non-random distribution within the X chromosomes. Caffeine and anthramycin produced only 0.5% and 3.1 cells with chromosome aberrations respectively. Potentiation of chromosome aberrations was observed when the anthramycin-treated cells were post-treated with caffeine. Caffeine potentiation presumably results from an inhibition of the cells to cycle and a failure to repair the effect of the mutagen on DNA.
D000818:Animals; D000876:Anthramycin / Q000494:pharmacology*; D001570:Benzodiazepinones / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D003670:Deer / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D007700:Kinetics; D012854:Sister Chromatid Exchange; D012867:Skin / Q000648:ultrastructure
D001570:Benzodiazepinones; D000876:Anthramycin; D002110:Caffeine