title: Extensive modification of protein amino groups by reductive addition of different sized substituents.
abstract: The amino groups of ovomucoid, lysozyme and ovotransferrin have been extensively alkylated by reacting the proteins with various carbonyl reagents in the presence of sodim borohydride. The extent of modification ranged from 40 to 100%. Essentially monosubstitution was obtained with acetone, cyclopentanone, cyclohexanone and benzaldehyde, while 20--50% disubstitution was obtained with N-butanal and nearby 100% disubstitution was obtained with formaldehyde. Both the methylated and isopropylated derivatives of all three proteins were soluble and retained almost full biochemical activities, but introduction of the larger substituents caused precipitation with lysozyme and ovotransferrin.
mesh_terms: D000478:Alkylation; D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D003207:Conalbumin; D004527:Egg Proteins; D007202:Indicators and Reagents; D009113:Muramidase; D010059:Ovomucin; D010084:Oxidation-Reduction
chemical_list: D000596:Amino Acids; D004527:Egg Proteins; D007202:Indicators and Reagents; D003207:Conalbumin; D010059:Ovomucin; D009113:Muramidase