[The influence of cytostatic and immunosuppressive methyl hydrazones on myelo- and lymphopoiesis in vitro (author's transl)].
The cytostatic and immunosuppressive N'-methyl-N'-beta-chloroethylbenzaldehyde hydrazones B1 and B2 inhibit the colony growth of mouse bone marrow cells and PHA-stimulated human lymphocytes in vitro in a dose-dependent manner. In the presence of B1, however, in contrast to B2, the inhibition of 3H-thymidine (3H-Tdr) uptake by the bone marrow cells and lymphocytes is insignificant. Two further benzaldehydrazones CyB4 and EB4 show little or no influence both on clony growth and nucleoside uptake. On the other hand, CyB4 inhibits the 3H-Tdr uptake by ConA- or LPS-stimulated mouse spleen cells to a gretaer degree than does B1 or B2, although CyB4 unlike B1 or B2 does not display any immunosuppressive effects in the mouse. These findings demonstrate that the 3H-Tdr method is less sensitive than the colony assays and is hence only of limited value as a measure of the vitro proliferation of mammalian cells treated with cytostatics.
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000187:drug effects*; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D006835:Hydrazones / Q000494:pharmacology*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008763:Methylhydrazines / Q000494:pharmacology; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D009150:Mustard Compounds / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D006835:Hydrazones; D008763:Methylhydrazines; D008934:Mitogens; D009150:Mustard Compounds; D013936:Thymidine
Einfluss von cytostatischen und immunosuppressiven Methylhydrazonen auf die Myelo- und Lymphopoese in vitro.