Effects of lead microparticles on the growth characteristics of the BHK21 fibroblast cell line.
BHK21 fibroblastic cells were exposed in vitro to lead microparticles produced by pyrolysis of the organic lead used as an antiknock agent in gasoline. Although non-cytolethal, 10 micrograms of lead/10(6) cells rapidly inhibited cell growth kinetics as well as [3H]thymidine and L-[3H]leucine incorporation by the BHK21 cell line. The growth rate inhibition provoked by chronic exposure to lead microparticles became reversible when the cells were cultured without the microparticulate pollutant. The cloning efficiency of BHK21 cells was impaired by lead concentrations above 1 mg/10(6) cells.
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D006224:Cricetinae; D005347:Fibroblasts / Q000187:drug effects; D007668:Kidney / Q000166:cytology; D007854:Lead / Q000633:toxicity*; D007930:Leucine / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D007854:Lead; D007930:Leucine; D013936:Thymidine