Preferential binding of S-phase proteins to temporally-characteristic units of replication in Physarum polycephalum.
Synchronous plasmodia of Physarum polycephalum were pulse-labeled with 3H-thymidine in early or late portions of the S-phase, and the binding capacity of the replicated DNA for isochronous S-phase plasmodial proteins assessed by nitrocellulose filter binding assay. Replication units replicating during the first one-third of the S-phase preferentially bind cytosol proteins present in plasmodia engaged in early S DNA replication, while late S replicating DNA exhibits a corresponding preferential binding of plasmodial proteins present only in late S plasmodia. Temporally-characteristic nascent replication units were isolated by Hydroxylapatite column chromatography and were found to contain binding sites for isochronous proteins.
D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification; D004261:DNA Replication; D005656:Fungal Proteins / Q000096:biosynthesis*; D006657:Histones; D007700:Kinetics; D008938:Mitosis; D008970:Molecular Weight; D009235:Myxomycetes / Q000378:metabolism*; D010804:Physarum / Q000378:metabolism*; D011485:Protein Binding; D013997:Time Factors
D005656:Fungal Proteins; D006657:Histones; D004247:DNA