Abolition of plasma growth hormone response to stress and of the circadian rhythm in pituitary-adrenal function in female rats with preoptic-anterior hypothalamic lesions.
The chronic effects of lesions placed in the medial or lateral preoptic-anterior hypothalamic area (PO-AHA) on plasma growth hormone (GH) and corticosterone (cpd B) responses to stress, non-stress levels of these hormones and somatic growth were studied in adult female rats. Responses to 3-min immobilization--blood withdrawal stress uere tested at 2 and 11 weeks and to 3-min ether--blood withdrawal at 5 weeks after surgery. Non-stress plasma GH levels were further assessed in serial blood samples collected at 4-h intervals during a 24-h period at 15 weeks, and cpd B levels were studied similarly for a 44-h period at 22 weeks. Rats with lateral PO-AHA lesions showed normal GH suppression and cpd B elevation in response to both stress procedures. In contrast, neither stressor suppressed plasma GH levels in rats with medial PO-AHA lesions, but both produced normal increments in cpd B. The 24-h mean non-stress level of plasma GH in the group with medial lesions was higher and that of the group with lateral lesions was lower than those of controls. Cpd B levels were reduced during the 44-h study, and circadian rhythmicity was abolished in rats with medial lesions. The group with lateral lesions showed normal rhythmicity in plasma cpd B levels, Linear growth was not affected in either lesioned group. The GH findings suggest that the medial but not lateral PO-AHA is essential for stress-induced inhibition of GH secretion and that this region may exert a tonic inhibitory influence on non-stress GH secretion. The data also suggest that the medial PO-AHA is importantly involved in producing the circadian rhythm in pituitary-adrenal function.
D000818:Animals; D002940:Circadian Rhythm; D003345:Corticosterone / Q000097:blood; D005260:Female; D013006:Growth Hormone / Q000097:blood*; D006801:Humans; D007031:Hypothalamus / Q000502:physiology*; D007032:Hypothalamus, Anterior / Q000502:physiology*; D010913:Pituitary-Adrenal System / Q000502:physiology*; D011301:Preoptic Area / Q000502:physiology*; D051381:Rats; D013315:Stress, Psychological / Q000502:physiology*
D013006:Growth Hormone; D003345:Corticosterone