Gene transfer by means of cell fusion I. Statistical mapping of the human X-chromosome by analysis of radiation-induced gene segregation.
Hybrid cells were obtained by virus-induced fusion of hamster cells with irradiated human cells. The analysis of such hybrids permits a study of the effects of lethal doses of radiation on human cells and provides a method of sub-chromosomal genetic mapping that is independent of karyological analysis. Radiation-induced chromosome exchanges are shown to be extremely localized, and a map of 4 X-linked genes is presented.
D000818:Animals; D002459:Cell Fusion / Q000528:radiation effects; D002874:Chromosome Mapping; D006224:Cricetinae; D005347:Fibroblasts; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology / Q000648:ultrastructure; D007621:Karyotyping; D008214:Lymphocytes / Q000528:radiation effects; D012730:Sex Chromosomes / Q000528:radiation effects