Radioimmunoassay of vinblastine and vincristine.
1 The cytotoxic agent, vinblastine, was conjugated to albumin, using the Mannich reaction. Rabbits immunized with two conjugates, containing differing amounts of hapten, produce antibodies which bound [3H]-vinblastine. 2 Antisera from one rabbit cross-reacted with both vinblastine and vincristine and were used to develop radioimmunoassays for measuring their concentration in plasma. 3 The antisera showed no cross-reactivity with other alkaloids or cytotoxic drugs and provided assays sensitive to a concentration of 2.1 ng vinblastine or 3.8 ng vincristine/ml of plasma added direct to the assay tubes. 4 This is sufficiently sensitive to permit the measurement of plasma vinblastine levels for up to 24 h after the intravenous administration of 15 mg of the drug.
D000818:Animals; D006801:Humans; D008722:Methods; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine; D013997:Time Factors; D014747:Vinblastine / Q000097:blood* / Q000276:immunology; D014750:Vincristine / Q000097:blood* / Q000276:immunology
D012710:Serum Albumin, Bovine; D014750:Vincristine; D014747:Vinblastine