A specific radio-immunological assay for prednisolone in plasma.
A new, specific radio-immunological assay (R.I.A.) for prednisolone in plasma is described. The R.I.A. incorporates a procedure whereby the endogenous interfering hydrocortisone (in de-proteinized plasma) reacts directly with Girard Reagent T, resulting in a derivative which does not cross react with the specific antibody for prednisolone. The assay is sensitive (quantitation levels are in the 5-10 ng/ml range). The specificity compares favorably with that of a high performance liquid chromatographic procedure.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D006801:Humans; D011239:Prednisolone / Q000097:blood* / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D013997:Time Factors
D000906:Antibodies; D011239:Prednisolone