title: Regulation of procollagen synthesis during the development of chick embryo calvaria. Correlation with procollagen mRNA content.
abstract: During the embryonic development of chick calvaria (membranous cranial bones), the relative rate of procollagen synthesis increased from about 12% of total protein synthesis on Day 10 to about 65% on Day 17. This increase is due to a 1.7-fold increase in the absolute rate of procollagen synthesis and a 3-fold decrease in the synthesis of noncollagenous proteins. The increase in procollagen synthesis is directly proportional to an increase in procollagen mRNA content per cell as measured either by cell-free translation or by hybridization with complementary DNA. The results indicate that translational control of procollagen mRNA does not play a substantial role during calvaria development and that the specialization in the synthesis of this protein is largely due to the loss or inactivation of mRNAs for noncollagenous proteins.
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D002642:Chick Embryo; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D011347:Procollagen / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D011347:Procollagen; D012333:RNA, Messenger