Electron microscope study of 1-(2,4-dichlorobenzyl)-1H-indazole-3-carboxylic acid, an exfoliative antispermatogenic agent, in the rat testis.
We were interested in discovering whether the antifertility agent, DICA [1-(2,4-dichlorobenzyl)-1-H-indazole-3-carboxylic acid] induced Sertoli cell tight junction damage. Testis were fixed in 1% lanthanum nitrite cacodylate-buffered 2% gluteraldehyde at various times following a single oral 100 mg/kg dose of DICA. In control animals adluminal lanthanum was never seen. At one and ten days following DICA treatment adluminal lanthanum was seen. This suggests that the Sertoli cell tight junctions are more permeable to lanthanum following DICA treatment.
D000727:Androgen-Binding Protein / Q000097:blood; D000818:Animals; D000988:Antispermatogenic Agents / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D007191:Indazoles / Q000494:pharmacology*; D007365:Intercellular Junctions / Q000187:drug effects*; D008297:Male; D011720:Pyrazoles / Q000494:pharmacology*; D051381:Rats; D012708:Sertoli Cells / Q000187:drug effects* / Q000648:ultrastructure; D013094:Spermatozoa / Q000187:drug effects
D000727:Androgen-Binding Protein; D000988:Antispermatogenic Agents; D007191:Indazoles; D011720:Pyrazoles