The effect of experimental uveitis on the uptake of prostaglandin E1 in the rabbit iris-ciliary body.
Disruption of the blood-aqueous barrier in rabbits was elicited by infrared irradiation of the iris or by alpha-melanocyte stimulating hormone (alpha-MSH) given subcutaneously. One group of animals was pretreated with topical imidazole before the injection of alpha-MSH. The aqueous flare response was followed and the rabbits were killed at the expected height of the uveitis. The uptake of 3H-prostaglandin E1 in the iris with the ciliary body was then determined and found to be significantly decreased in the rabbits in which alpha-MSH had caused a severe damage of the blood-aqueous barrier. When alpha-MSH caused a more moderate aqueous flare response the prostaglandin uptake was on the contrary significantly increased. Pretreatment of the animals with topical imidazole enhanced parallelly the prostaglandin uptake and the aqueous flare response to alpha-MSH. Topical imidazole per se was found to increase the accumulation of prostaglandin. The prostaglandin uptake values were, however, unchanged in eyes in which infrared irradiation of the iris induced a moderate flare response.
D000287:Administration, Topical; D000818:Animals; D002924:Ciliary Body / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D007093:Imidazoles / Q000008:administration & dosage / Q000494:pharmacology; D007259:Infrared Rays; D007279:Injections, Subcutaneous; D007498:Iris / Q000378:metabolism* / Q000528:radiation effects; D009074:Melanocyte-Stimulating Hormones / Q000008:administration & dosage / Q000494:pharmacology; D011458:Prostaglandins E / Q000378:metabolism*; D011817:Rabbits; D014605:Uveitis / Q000378:metabolism*
D007093:Imidazoles; D011458:Prostaglandins E; D009074:Melanocyte-Stimulating Hormones