[Preliminary lymphocyte activation by a mitogen as a condition for demonstrating the ability of the Fab-fragment of normal IgG to inhibit lymphocyte transformation].
Peculiarities attending inhibition of the PHA-induced blast-cell transformation of human lymphocytes by F(ab')2 fragment of rabbit IgG were studied. It was shown that the fragment did not affect the intensity of blast-cell transformation if the lymphocytes were preliminarily incubated with the fragment for 24 h at 37 degrees or 4 degrees C and then transferred to the fresh medium containing PHA. However, if the fragment was added to the cells 24 or 48 h following PHA it produced a significant inhibition of the blast-cell transformation. These data may indicate that F(ab')2 fragment interferes with the lymphocyte transformation only when the cells are already activated with PHA.
D000818:Animals; D001666:Binding Sites, Antibody; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D011817:Rabbits / Q000276:immunology; D013997:Time Factors
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D010835:Phytohemagglutinins
Predvaritel'naia aktivatsiia limfotsitov mitogenom kak uslovie dlia proiavleniia sposobnosti Fab-fragmentov normal'nogo IgG ingibirovat' transformatsiiu limfotsitov.