Conversion of schistosome cercariae to schistosomula in serum-supplemented media, and subsequent culture in vitro.
Both Schistosoma japonicum and S. mansoni cercariae, axenized by washing in NCTC 109, transformed to schistosomula when incubated directed in NCTC 109 containing 2--50% human or 50% rabbit serum at 37 degrees C. The resultant schistosomula were grown in vitro to adults which mated, although the females did not produce eggs. The cercaria-schistosomulum transformation took place at a slower rate in heat inactivated serum than in unheated serum.
D000818:Animals; D001769:Blood; D003470:Culture Media; D006801:Humans; D007814:Larva; D008675:Metamorphosis, Biological; D011817:Rabbits; D012547:Schistosoma / Q000254:growth & development*; D012550:Schistosoma mansoni / Q000254:growth & development
D003470:Culture Media