[Studies on the detection of clonazepam and its main metabolites considering in particular thin-layer chromatography discrimination of nitrazepam and its major metabolic products (author's transl)].
The article describes analytical methods concerning screening tests for clonazepam and nitrazepam and the 7-amino derivatives. Further a detailed method is reported for the separation and identificatin of the benzodiazepine pair de. The method described permits a sharp separation and the nitro compounds with TiCl3 on the plate and forming the 7-acetamido derivatives by subsequent separation in the second dimension with ethyl acetate/acetic anhydride. The method described permits a sharp separation and highly sensitive detection by diazotization and coupling with Bratton-Marshall reagent. Amounts as low as 0.02 microng per spot can be detected. Besides preparation methods are reported for 7-aminoclonazepam, 7-acetaminoclonazepam, 2-amino-2'-chloro-5-nitrobenzophenone and 2,5-diamino-2'-chlorobenzophenone. Also spectral data (UV, IR, MS) and a literature review are given.
D000586:Amination; D001570:Benzodiazepinones / Q000652:urine*; D001711:Biotransformation; D002855:Chromatography, Thin Layer; D002998:Clonazepam / Q000652:urine*; D013058:Mass Spectrometry; D008722:Methods; D009567:Nitrazepam / Q000652:urine*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D001570:Benzodiazepinones; D002998:Clonazepam; D009567:Nitrazepam
Untersuchungen zum Nachweis von Clonazepam und seinen Hauptmetaboliten unter besonderer Berücksichtigung der dünnschichtchromatographischen Unterscheidung von Nitrazepam und dessen wichtigsten Stoffwechselprodukten