Marrow flask cultures -- a system for examining early erythropoietic differentiation events.
A technique for observing in flask cultures the differentiation and proliferation of murine CFU-S into each of the three myeloid lines is described. The differentiation of cells along the erythroid pathway is arrested at a very early stage making it possible to obtain large numbers of primitive erythropoietic cells derived from adult murine bone marrow. The pattern of differentiation obtained from these BFU-E suggests that the capacity for granulopoiesis is usually lost before a committment to either megakaryopoiesis or erythropoiesis is made.
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice