[In-vitro evaluation of morphine dependence. III. Response of the rat ileum to ACh].
The kinetic of cholinergic receptor systems was studied in an in vitro model for opiate tolerance and dependence. The isolated ileum of rate chronically treated with morphine and sacrificed at different time intervals and in different conditions (with and without abstinence signs) was used. In evaluating the response of this preparation to doses of ACh ranging from 5.5 x 10(-10) to 5.5 x 10(-7), no statistically significant response was found to the cholinergic receptor system in conditions of abstinence and non-abstinence in vivo and in vitro. On the other hand, statistically significant differences are related to duration of treatment in vivo. The pattern for these responses is similar to the one observed in the fundus (Note I) and it can be considered as an expression of morphine-induced tolerance.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D006801:Humans; D007082:Ileum / Q000187:drug effects*; D009020:Morphine / Q000494:pharmacology*; D009021:Morphine Dependence / Q000175:diagnosis*; D051381:Rats; D011950:Receptors, Cholinergic / Q000187:drug effects*
D011950:Receptors, Cholinergic; D009020:Morphine; D000109:Acetylcholine
Valutazione in vitro della dipendenza da morfina. -III) Risposta all'ACh dell'ileo di ratto.