Rotenone inhibition of tubulin self-assembly.
Rotenone effectively inhibits the in vitro formation of microtubules from tubulin containing or lacking microtubule-associated proteins. In both cases a concentration of rotenone equal to that of tubulin present completely blocks assembly. The inhibition can be reversed by the addition of dimethylsulfoxide or by removing rotenone with charcoal.
D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D002606:Charcoal / Q000494:pharmacology; D003078:Colchicine / Q000037:antagonists & inhibitors; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006023:Glycoproteins / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D012402:Rotenone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism*
D006023:Glycoproteins; D014404:Tubulin; D012402:Rotenone; D002606:Charcoal; D003078:Colchicine; D004121:Dimethyl Sulfoxide