[Effect of pellet size on respiratory activity and motility of cryopreserved spermatocytes].
The effect of different pellet volumes at freeze preservation in human semen was studied. It was measured the motility of spermatozoa during an incubation time of several hours at 37 degrees C and the alteration of oxygen consumption after adding rotenone, succinate, digitonine or 2,4-dinitrophenol. - The motility of cryopreserved spermatozoa does not show significant differences after preservation procedure. In respect of oxygen consumption the smallest differences from native material were obtained at a pellet volume of 0,2 to 0,3 cm3. Explanations for this behaviour are discussed.
D004072:Digitonin / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D012034:Refrigeration; D012402:Rotenone / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects; D013090:Spermatocytes / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D013386:Succinates / Q000494:pharmacology
D004140:Dinitrophenols; D013386:Succinates; D012402:Rotenone; D004072:Digitonin
Einfluss der Pelletgrösse auf die Atmungsaktivität sowie Motilität kryokonservierter humaner Spermienzellen.