Preferential neurotoxicity of pentobarbital on nerve and glial cells in culture.
The effect of pentobarbital was studied in a mixed population of nerve and glial cells dissociated from brains of 7-day chick embryos and maintained in culture. Pentobarbital-Na was added in various concentrations ranging from 5 X 10(-5) M to 1 X 10(-3) M. The neuronal density was monitored by counting the neurons, neuronal identity was established by staining for Nissl Bodies and acetylcholinesterase. Over a culture period of 3 weeks, it was found that the barbiturate exerts a preferential dose-dependent cytotoxic effect on neurons.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010424:Pentobarbital / Q000494:pharmacology*; D013997:Time Factors
D010424:Pentobarbital