Improved assay of neurotoxic esterase for screening organophosphates for delayed neurotoxicity potential.
The assay of neurotoxic esterase (NTE) in brains taken from dosed hens enables potential neurotoxicity of organophosphate pesticides, plasticers, etc. to be assessed. The original assay [Johnson, M.K. Biochem. J. 114, 711-717 (1969)] has been simplified to eliminate centrifugation and transfer steps and both the selectivity and the sensitivity have been increased. The procedures necessary to obtain stable reagent stocks are described.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002645:Chickens; D004950:Esterases / Q000378:metabolism*; D007531:Isoflurophate / Q000031:analogs & derivatives / Q000138:chemical synthesis; D008722:Methods; D009943:Organophosphorus Compounds / Q000009:adverse effects*; D010261:Paraoxon
D009943:Organophosphorus Compounds; D007531:Isoflurophate; D004950:Esterases; D010261:Paraoxon