Platelet interaction with modified articular cartilage. Its possible relevance to joint repair.
During studies concerned with the platelet-collagen interaction, it was observed that platelets did not adhere to bovine or human articular cartilage and that cartilage did not induce platelet aggregation in vivo or in vitro. To study the mechanism responsible for this observation, the role of proteoglycans was examined. Purified cartilage collagen proved to be fully active as a platelet aggregant. Addition of small amounts of proteoglycan subunit (PGS) blocked platelet aggregation, whereas chondroitin sulfate, a major glycosaminoglycan component of cartilage matrix, impaired platelet aggregation only at concentrations which resulted in a marked increase in viscosity. Moreover, PGS abolished aggregation of platelets by polylysine but did not prevent aggregation by ADP, suggesting that PGS may block strategically placed lysine sites on the collagen molecule. Treatment of fresh articular cartilage with proteolytic enzymes rendered the tissue active as a platelet aggregant. In vivo experiments demonstrated that surgical scarification of rabbit articular cartilage does not result in adhesion of autologous platelets. Treatment of rabbit knee joints with intraarticular trypsin 1 wk before the injection of blood resulted in adhesion and aggregation of platelets on the surface of the lesions. Since there is evidence from other studies that some degree of cartilage healing may take place after initiation of an inflammatory response, it is postulated that induction of platelet-cartilage interaction may eventuate in cartilage repair.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology; D002417:Cattle; D002809:Chondroitin Sulfates / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D006801:Humans; D007718:Knee Injuries / Q000139:chemically induced; D010206:Papain; D010974:Platelet Aggregation / Q000187:drug effects; D011107:Polylysine / Q000494:pharmacology; D011509:Proteoglycans / Q000494:pharmacology; D011817:Rabbits; D014357:Trypsin
D011509:Proteoglycans; D011107:Polylysine; D000244:Adenosine Diphosphate; D002809:Chondroitin Sulfates; D003094:Collagen; D014357:Trypsin; D010206:Papain