The role of blood clotting factor V in the conversion of prothrombin and a decarboxy prothrombin into thrombin.
Purified PIVKA-II exhibits some factor II (prothrombin) activity in the one-stage coagulation assay and this factor II activity does not come from residual amounts of factor II but originates from PIVKA-II itself. It is shown that PIVKA-II is converted by a normal prothrombinase complex (factor Va and factor Xa adsorbed onto a phospholipid interface) more readily than by phospholipids and factor Xa alone. This suggests that binding between PIVKA-II and factor Va is an essential feature in the formation of the enzyme . substrate complex and from this we infer that a direct interaction between factor Va and prothrombin plays a rôle in the prothrombinase . prothrombin complex.
D000818:Animals; D001777:Blood Coagulation; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D005165:Factor V / Q000378:metabolism*; D005170:Factor X / Q000378:metabolism; D007700:Kinetics; D010743:Phospholipids / Q000494:pharmacology; D011516:Prothrombin / Q000378:metabolism*; D013917:Thrombin / Q000378:metabolism*
D010743:Phospholipids; D005165:Factor V; D011516:Prothrombin; D005170:Factor X; D013917:Thrombin; D002118:Calcium