Reliable detection of cystic fibrosis in skin-derived fibroblast cultures.
The specific induction of alkaline phosphatase with Tamm-Horsfall glycoprotein, isoproterenol, and theophylline in skin-derived fibroblast cultures from patients with cystic fibrosis permits one to reliably discriminate between cystic fibrosis patients on the one hand, and heterozygotes and normals on the other. It was found the fibroblast-like and intermediary types of amniotic fluid-derived cells behave essentially like skin-derived fibroblasts. These findings imply that if different amniotic fluid cell types can be reliably separated, prenatal diagnosis of cystic fibrosis should become feasable in the near future.
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000175:diagnosis*; D004790:Enzyme Induction; D005260:Female; D005347:Fibroblasts / Q000201:enzymology*; D006023:Glycoproteins; D006579:Heterozygote; D006801:Humans; D007545:Isoproterenol; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012867:Skin / Q000166:cytology; D013806:Theophylline
D006023:Glycoproteins; D013806:Theophylline; D000469:Alkaline Phosphatase; D007545:Isoproterenol