Hybridization properties of human X-chromosomal RNA transcripts from murine--human hybrids.
A human--mouse hybrid cell line which has retained the human X chromosome and a fragment of chromosome 2 contains RNA sequences transcribed from human DNA. Nuclear RNA enriched for human sequences was prepared from this hybrid cell line by a multistep hybridization procedure to mouse and human DNA immoblized on nitrocellulose filters. The properties of this RNA were analyzed by RNA--DNA hybridization techniques. The results indicate that we have prepared a RNA fraction enriched for sequences homologous to repeated DNA sequences of the human X chromosome.
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics*; D012730:Sex Chromosomes; D014158:Transcription, Genetic; D014960:X Chromosome
D012313:RNA; D004247:DNA