Denaturation of mouse satellite DNA upon melting of chromatin in solution.
The denaturation of mouse satellite DNA upon melting of chromatin in solution of low ionic strength has been studied. A procedure for preparation of partially denaturated chromatin was developed which enabled the isolation of double-stranded (non-denatured) DNA sequences according to their thermal stability in chromatin. The content of mouse satellite DNA in these DNA sequences was determined by hybridization with RNA, complementary to satellite DNA in order to find the temperature interval of denaturation of satellite DNA. It was found that the melting temperature of satellite DNA in chromatin was lower than that of the total DNA. The results are discussed in relation to previously reported anomalous behaviour of satellite DNA upon melting of chromatin on hydroxyapatite.
D000818:Animals; D001483:Base Sequence; D002286:Carcinoma, Ehrlich Tumor; D002843:Chromatin; D004247:DNA; D004276:DNA, Satellite; D005557:Formaldehyde; D006358:Hot Temperature; D006882:Hydroxyapatites; D051379:Mice; D009691:Nucleic Acid Denaturation; D009994:Osmolar Concentration; D011485:Protein Binding; D012996:Solutions
D002843:Chromatin; D004276:DNA, Satellite; D006882:Hydroxyapatites; D012996:Solutions; D005557:Formaldehyde; D004247:DNA