[Fractionation of "tricholysine" by isoelectric focusing].
A possibility to fractionate fibrinolytically and thrombolytically active complex "tricholysin" into five components, differing in iso-points and in enzymatic activity, is demonstrated by means of isoelectric focusing using ampholine solution within pH range 3.0-10.0. Homogenous fraction IV (isopoint 6.8-7.0) has a low caseinolytic activity and a high specific fibrinolytic and esterase activities. This fraction is characterized with a high ability of plasminogen activation. Serine, threonine, alanine and valine are found to prevail in amino acid composition of the fraction IV, its molecular weight being 39000.
D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D004798:Enzymes / Q000302:isolation & purification*; D004950:Esterases / Q000302:isolation & purification; D005343:Fibrinolytic Agents / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D003904:Mitosporic Fungi; D008970:Molecular Weight; D010447:Peptide Hydrolases; D010960:Plasminogen Activators / Q000302:isolation & purification
D000596:Amino Acids; D004798:Enzymes; D005343:Fibrinolytic Agents; D004950:Esterases; D010447:Peptide Hydrolases; C014864:tricholysine; D010960:Plasminogen Activators
Fraktsionirovanie "trikholizina" metodom izoelektricheskogo fokusirovaniia