[Isolation and structure of an external glycoprotein of the cell wall of Torulopsis candida].
The treatment with ethylenediamine of Torulopsis candida yeast cell walls has permitted to isolate a mannoprotein. It has been possible to collect this glycoprotein in a pure state after separation of the different hydrosoluble products by gel filtrations and paper electrophoresis. This compound contains 62 per cent of mannose, 3 per cent of N-acetylglucosamine and 31 per cent of amino acids. The mannose units are attached by 1 leads to 2 linkage, short oligosaccharides are bound to serine of the peptide chain by O-glycosidic linkages while polysaccharides are attached to asparagine of this peptide chain by a N glycosidic linkages probably through the intermediate of chitobiose.
D000596:Amino Acids / Q000032:analysis; D002175:Candida / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000032:analysis*; D006023:Glycoproteins / Q000302:isolation & purification; D008358:Mannose / Q000032:analysis; D008968:Molecular Conformation
D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D008358:Mannose
Isolement et structure dune glycoprotéine superficielle de la paroi de Torulopsis candida.