[Chromatography of membrane proteins in Triton-X 100. II) Affinity chromatography of membrane proteins on Con-A linked to Sepharose in Triton-X 100].
The Authors have made chromatographs using Con-A Sefaroso columns, varying the percent of Triton-X 100 and the proteic concentration. With proteic concentrations varying from 0.4 to 4mg/ml and in solutions containing 0.5 to 1% of Triton-X 100 with the pH value remaining constant at 8 - solubilization of membrane protein does occur.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000302:isolation & purification*; D008861:Microsomes / Q000032:analysis; D017830:Octoxynol; D011092:Polyethylene Glycols; D051381:Rats; D012685:Sepharose; D012995:Solubility; D013687:Telencephalon / Q000032:analysis
D008565:Membrane Proteins; D003208:Concanavalin A; D011092:Polyethylene Glycols; D017830:Octoxynol; D012685:Sepharose
Cromatografia delle proteine di membrana in Triton-X 100. II) Cromatografia di affinità delle proteine di membrana su Con-A legata al Sefaroso in Triton-X 100.