Two-step binding of eukaryotic ribosomes to brome mosaic virus RNA3.
Although brome mosaic virus RNA3 has only one translatable cistron, it can bind two 80S ribosomes at initiation. One ribosome binds at the first AUG codon (base 92-94). The other binds nearer the 5' end at an entry or holding site. Disome formation is thus unrelated to a silent cistron approximately 1,000 bases downstream.
D001483:Base Sequence; D001665:Binding Sites; D002474:Cell-Free System; D003062:Codon; D009029:Mosaic Viruses / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D003062:Codon; D012333:RNA, Messenger; D012367:RNA, Viral