The involvement of microfilaments and microtubules in the lateral mobility of lectin binding sites of normal and brachypod mouse limb mesenchyme.
It has been demonstrated that differences exist in the lateral mobility of Con A- and WGA-binding sites in the membranes of normal and brachypod mouse limb mesenchymal cells (Hewitt et al., 1978). The work presented here investigates the involvement of microtubules and microfilaments as mediators of binding site mobility in this system. Treatment of cells with colchicine suggests that microtubules are not involved in the mobility of either type of lectin-binding site. Disruption of microfilaments with cytochalasin B prevents the redistribution of Con A-binding sites but not those of WGA. the results were found to be the same for both genotypes. This suggests that the differences which have been found between genotypes are related to some mechanism of restraining the lateral mobility of lectin binding sites other than by attachment to microtubules and microfilaments.
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003593:Cytoplasm / Q000502:physiology*; D003599:Cytoskeleton / Q000502:physiology*; D005855:Germ Layers / Q000502:physiology*; D006614:Hindlimb / Q000196:embryology; D051379:Mice; D008870:Microtubules / Q000502:physiology*; D009154:Mutation; D011952:Receptors, Concanavalin A / Q000502:physiology*; D011955:Receptors, Drug / Q000502:physiology*
D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D003208:Concanavalin A; D003571:Cytochalasin B; D003078:Colchicine