Rapid antibody production and sample preparation for radioimmunoassay of prostaglandin A.
A dialysis equilibrium method for plasma or tissue preparation for radioimmunoassay of prostaglandins is presented and compared to the silica gel column technique. The plasma or tissue is extracted, dried, redissolved in buffer and dialyzed for further purification before assay. Results comparing both methods shows that dialysis equilibrium greatly simplifies the silica gel column technique yet preserves specificity, sensitivity and reproducibility. In addition a rapid micromethod for the development of a specific antibody to prostaglandin A is detailed.
D000818:Animals; D000906:Antibodies; D002850:Chromatography, Gel / Q000379:methods; D003956:Dialysis; D006801:Humans; D008722:Methods; D008832:Microchemistry; D011454:Prostaglandins A / Q000097:blood* / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods
D000906:Antibodies; D011454:Prostaglandins A