[Interference of some detergent micelles with the titration of protein].
We have examined the possible interferences with spectrophotometric protein determination of some detergents commonly used for the solubilization of membrane proteins. The results show that some mixtures of Triton X100 with SDS or DOC, at given concentrations, interfere with BSA determination by the method of Lowry.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003840:Deoxycholic Acid; D008623:Mercaptoethanol; D008722:Methods; D017830:Octoxynol; D000077423:Polidocanol; D011092:Polyethylene Glycols; D012710:Serum Albumin, Bovine / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents
D013501:Surface-Active Agents; D003840:Deoxycholic Acid; D000077423:Polidocanol; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D008623:Mercaptoethanol; D017830:Octoxynol
Interferenza di alcune miscele di detergenti con il dosaggio di proteine.