[Relation between ATP and the sum of ATP + ADP levels in rat muscle and liver and NH4Cl levels in fodder].
The experiment was carried out on 85 female breed Wistar rats divided into five groups. All groups of animals except 1 got NH4Cl in the feed in increasing amounts: 2-nd group 1%, 3-rd 2%, 4-th 4% and 5-th group 8%. After two weeks the animals were killed and then the slides of muscles and liver were taken. The levels of ATP, ADP and sum of ATP and ADP in the tissues were marked. In both tissues it was shown that there was an increase of ATP level in the case of higher level of NH4Cl up to 2% and a decrease in cases of lower concentration. Probably the decrease was caused by augmented hydrolysis of ATP to ADP, which is confirmed by the increase of ADP level. We could suppose, that small amounts of NH4Cl (1--2%) accelerate the synthesis of ATP in muscles and liver. The disposal of ADP levels by augmented concentration NH4Cl in fodder is different in both tissues. In the case of muscles there was a decrease of their level to 3-rd group (2% NH4Cl) and an increase to 5-th group. We could presume that further rise of level depends on the penetration of ADP from mitochondria to cytoplasma. The disposal of ADP level in liver is similar to the disposal of ATP. We can see a rise to 3-rd group and a fall to 4-th group. This decrease probably is connected with the production of phosphocreatine needed as a source of energy for the work of muscles.
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000643:Ammonium Chloride / Q000032:analysis* / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D005260:Female; D008099:Liver / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D051381:Rats
D000643:Ammonium Chloride; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate
Zalezność poziomu ATP i sumy ATP + ADP w mieśniach i watrobie szczurów od poziomu NH4Cl w paszy.