Isolation of pyrimidine isostichs from chick erythrocyte DNA.
The isolation of pyrimidine oligonucleotides (isostichs) from chick erytyrocyte DNA was accomplished on a preparative scale with the goal of providing starting material for further chemical modifications. The DNA was degraded by the method of Burton and Petersen and the isostichs were obtained via paper chromatography and ion-exchange chromatography. The various isostichs were further fractionated into base compositional isomers, the frequency of which was determined as mole percent of total pyrimidines. Although this work was not intended to be an analytical study, the large quantities of DNA used allowed the measurement of most compositional isomers with an accuracy of 5% of the listed values. The data for long oligothymidylates are less reliable. The results of the present study revealed the anticipated bias in the distribution of pyrimidine isostichs in favor of longer chain lengths as compared to that expected for a random distribution. Isostichs above chain length 10 occur in amounts approximately 7 times greater than calculated.
D000818:Animals; D002645:Chickens; D002852:Chromatography, Ion Exchange / Q000379:methods; D002854:Chromatography, Paper / Q000379:methods; D004247:DNA / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis*; D009841:Oligonucleotides / Q000097:blood / Q000302:isolation & purification; D011742:Pyrimidine Nucleotides / Q000097:blood* / Q000302:isolation & purification
D009841:Oligonucleotides; D011742:Pyrimidine Nucleotides; D004247:DNA