Serum requirement for growth and immunoglobulin synthesis by cultured human lymphocyte lines.
Swine serum is an inexpensive substitute for foetal calf serum for lymphocyte culture. It gives an increased yield of Ig from 1788 cells and permits the large scale production of cells for Ig mRNA isolation.
D000818:Animals; D001769:Blood; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D005312:Fetal Blood; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D013552:Swine
D003470:Culture Media; D007136:Immunoglobulins; D012333:RNA, Messenger