title: Improved assay of neurotoxic esterase for screening organophosphates for delayed neurotoxicity potential.
abstract: The assay of neurotoxic esterase (NTE) in brains taken from dosed hens enables potential neurotoxicity of organophosphate pesticides, plasticers, etc. to be assessed. The original assay [Johnson, M.K. Biochem. J. 114, 711-717 (1969)] has been simplified to eliminate centrifugation and transfer steps and both the selectivity and the sensitivity have been increased. The procedures necessary to obtain stable reagent stocks are described.
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002645:Chickens; D004950:Esterases / Q000378:metabolism*; D007531:Isoflurophate / Q000031:analogs & derivatives / Q000138:chemical synthesis; D008722:Methods; D009943:Organophosphorus Compounds / Q000009:adverse effects*; D010261:Paraoxon
chemical_list: D009943:Organophosphorus Compounds; D007531:Isoflurophate; D004950:Esterases; D010261:Paraoxon