An improved method for the extraction of cholesterol and phospholipids from the bovine spinal cord.
A pharmacopoeial grade of cholesterol and a separate phospholipid fraction can be obtained by this simple extraction procedure of bovine spinal cord. The tissue is collected in 5% (Wt/V) formalin. It is then separated from the formalin solution, minced and dried in a hot-air oven at 105 degrees C. Total lipids are extracted with petroleum ether. The cholesterol is separated from the phospholipids by extraction with a mixture of petroleum ether and acetone. The method allows for not only the recovery of the phospholipids but also a high yield of cholesterol without using saponification or tedious purification procedures.
D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000302:isolation & purification*; D013058:Mass Spectrometry; D008722:Methods; D008970:Molecular Weight; D010743:Phospholipids / Q000302:isolation & purification*; D013116:Spinal Cord / Q000032:analysis*
D010743:Phospholipids; D002784:Cholesterol