Partial purification and characterization of two Cladosporium herbarum allergens.
Two important Cladosporium herbarum allergens (Ag-54 and Ag-32) were partly purified by means of diafiltration, gel filtration and isoelectric focusing. Modifications of quantitative immunological techniques, including autoradiography, were used as assay systems for allergenic activity and to provide a basis for evaluation of the immunological purity of the allergens. Ag-54 appeared to have a molecular weight of approximately 25,000 daltons and an isoelectric point of 5.0. The molecular weight of Ag-32 appeared to be approximately 13,000 daltons. It consisted of 5 molecular species differing in isoelectric points from 3.4 to 4.4 in intervals of approximately 0.25 pH units. These variants of Ag-32 seemed to be antigenically and allergenically identical.
D000485:Allergens / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002963:Cladosporium / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D007525:Isoelectric Focusing; D003904:Mitosporic Fungi / Q000276:immunology*; D008970:Molecular Weight; D011852:Radioallergosorbent Test
D000485:Allergens