Thiourea reverses cross-links and restores biological activity in DNA treated with dichlorodiaminoplatinum (II).
Cis and trans dichlorodiaminoplatinum (II) compounds bind to DNA and form DNA cross-links, which are usually considered to be irreversible. Thiourea can reverse these cross-links without any apparent breakdown of the DNA. In addition, cis- and trans-Pt (II) treatment of lambda decreases its transfectivity. After suitable incubation with thiourea, full transfectivity of Pt(II)-treated lambda DNA can be restored.
D000818:Animals; D002460:Cell Line; D003090:Coliphages; D004247:DNA / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D007939:Leukemia L1210; D009944:Organoplatinum Compounds / Q000037:antagonists & inhibitors*; D013329:Structure-Activity Relationship; D013890:Thiourea / Q000494:pharmacology*
D004273:DNA, Neoplasm; D004279:DNA, Viral; D009944:Organoplatinum Compounds; D004247:DNA; D013890:Thiourea