Three-dimensional structure of the lipovitellin-phosvitin complex from amphibian oocytes.
Microcrystals of the lipoprotein-phosphoprotein complex which are found in the oocytes of Xenopus laevis were examined using electron microscopy. Analysis of Fourier transforms of the images of the (010) and (001) projections showed the space group to be P2(1)22(1). Ten projections were combined to produce a map of the complex having about 20 A resolution. The lipoprotein complex consists of two subunits related by a local twofold symmetry axis. The density was averaged around the local symmetry and reasonably well defined structural domains can be seen in the resulting model.
D000818:Animals; D003461:Crystallography; D004527:Egg Proteins; D005260:Female; D008074:Lipoproteins; D008854:Microscopy, Electron; D008958:Models, Molecular; D008968:Molecular Conformation; D009865:Oocytes; D010750:Phosphoproteins; D010774:Phosvitin; D011487:Protein Conformation; D014981:Xenopus
D004527:Egg Proteins; D008074:Lipoproteins; D010750:Phosphoproteins; D010774:Phosvitin; C001549:lipovitellin