[Effect of chemical carcinogens on the ultrastructure of cultured differentiating myogenic cells].
The electron microscope studies have been carried out on primary monolayer tissue culture obtained from body tissues of rat and C3H mouse embryos. The cells of tissue culture were mainly myoblasts and fibroblast-like cells. The cultures were treated with two different carcinogenic substances--benz(a)-pyrene (BP) and methylnitrosonitroguanidine (MNNG). The changes were uniform and showed some alterations in formation of cell complexes, the inhibition of development and maturing of muscle elements, and distrophy of cytoplasmic organelles. The revealed distinction in morphological reaction of myogenic cells to the effect of BP and MNNG were wave-like myofibrillar structures in MNNG-treated cultures.
D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D046508:Culture Techniques; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D051379:Mice; D008854:Microscopy, Electron; D009132:Muscles / Q000187:drug effects* / Q000196:embryology / Q000648:ultrastructure; D009210:Myofibrils / Q000187:drug effects; D009940:Organoids / Q000187:drug effects; D051381:Rats
D001580:Benzopyrenes; D008769:Methylnitronitrosoguanidine
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