Circadian clock in culture: N-acetyltransferase activity of chick pineal glands oscillates in vitro.
N-Acetyltransferase activity was measured in organ-cultured chick pineal glands. A circadian rhythm of enzyme activity persisted in cultured glands for up to 4 days. The phase of the rhythm in vitro closely approximates its phase in vivo. These observations demonstrate that the pineal gland of chicks contains (or is) a self-sustained circadian oscillator.
D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D002940:Circadian Rhythm; D003624:Darkness; D010870:Pineal Gland / Q000201:enzymology / Q000502:physiology*
D000123:Acetyltransferases