title: Purification and chemical characterization of polysaccharides obtained from Lampteromyces japonicus by concanavalin A-sepharose affinity chromatography.
abstract: Two classes of neutral polysaccharide which could not be separated from each other by conventional methods were isolated from the fungus, Lampteromyces japonicus, by affinity chromatography using concanavalin A-Sepharose. The polysaccharide retained on the concanavalin A-Sepharose column was eluted with 0.05 M methyl alpha-D-mannopyranoside and appeared to be alpha-mannan, while that which passed through the column was virtually all beta-glucan. Both polysaccharides were subjected to Smith-type degradation, methylation, acetolysis and glucosidase treatment. The results indicated that the alpha-mannan contained predominantly alpha-(1 leads to 2)-linked side chains branching from an alpha-(1 leads to 6)-linked backbone at the (1 leads to 2,6)-linked mannopyranosyl residues. Galactose was attached to approximately one-quarter of the non-reducing mannose terminals. The beta-glucan seemed to contain mainly (1 leads to 6)-linked side chains branching from a (1 leads to 3)-linked backbone at the (1 leads to 3,6)-linked glucopyranosyl residues.
mesh_terms: D000363:Agaricales; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D003208:Concanavalin A; D005690:Galactose / Q000032:analysis; D008358:Mannose / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification*; D012685:Sepharose
chemical_list: D011134:Polysaccharides; D003208:Concanavalin A; D012685:Sepharose; D008358:Mannose; D005690:Galactose