Biotransformation of some pyrazole derivatives to glutathione conjugates in rat liver subcellular fractions.
During incubation of antipyrine, but not amidopyrine, 4-aminoantipyrine and 4-leucylaminoantipyrine, with rat liver microsomaland cytosol fractions in the presence of NADPH-generating system a reactive metabolite, which binds with glutathione is formed. The chemical nature of the metabolically activated intermediate is not known; it is suggested that the putative reactive metabolite responsible for this binding could be an epoxide.
D000818:Animals; D000983:Antipyrine / Q000378:metabolism; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004852:Epoxy Compounds / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D011720:Pyrazoles / Q000378:metabolism*; D051381:Rats; D013343:Styrenes / Q000378:metabolism
D004852:Epoxy Compounds; D011720:Pyrazoles; D013343:Styrenes; D003577:Cytochrome P-450 Enzyme System; D005978:Glutathione; D000983:Antipyrine