Detection of double-stranded RNA in Semliki Forest virus-infected cells by an indirect solid phase radioimmunoassay: an assay for interferon.
An indirect solid phase radioimmunoassay using rabbit antibodies to the synthetic double-stranded RNA poly rI-rC and 125I-labelled sheep anti-rabbit IgG could detect 1 ng of poly rI-rC. This assay could also detect the presence of double-stranded RNA in Semliki Forest virus-infected chick fibroblasts and can be used as an assay for interferon activity.
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D046508:Culture Techniques; D007106:Immune Sera; D007372:Interferons / Q000032:analysis* / Q000494:pharmacology; D011070:Poly I-C / Q000276:immunology; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D011863:Radioimmunoassay / Q000379:methods*; D012672:Semliki forest virus / Q000032:analysis* / Q000378:metabolism
D007106:Immune Sera; D012367:RNA, Viral; D007372:Interferons; D011070:Poly I-C