Radioimmunoassay of amitriptyline and nortriptyline in body fluids.
A radioimmunoassay for the measurement of amitriptyline and nortriptyline in serum, saliva, and urine is described. The sensitivity of the assay is such that 0.5 ng/ml of drug can be measured, although the assay does not distinguish between amitriptyline and nortriptyline. Other tricyclic compounds cross-react with the antiserum to varying degrees, but the metabolites of amitriptyline did not significantly cross-react. Total tricyclic compounds were detected in serum and saliva after single oral doses of amitriptyline, although the absorption was slow.
D000639:Amitriptyline / Q000032:analysis*; D000818:Animals; D003429:Cross Reactions; D006801:Humans; D007099:Imipramine; D009661:Nortriptyline / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D012463:Saliva / Q000032:analysis; D012756:Sheep / Q000276:immunology
D000639:Amitriptyline; D009661:Nortriptyline; D007099:Imipramine