Separation of proximal tubule cells from suspensions of rat kidney cells by free-flow electrophoresis.
Suspensions of rat kidney cells obtained by disaggregation of the kidney with 0.25% trypsin were separated by electrophoresis. Previously, we found a correlation between cells with histochemically demonstrable alkaline phosphatase (HDAP) and cells with brush borders which established that HDAP is a useful marker for rat proximal tubule cells (Kreisberg et al., '77). The starting suspension of cells for electrophoresis consisted of 38.4 +/- 5.7% nucleated cells with HDAP, 39.8 +/- 5.7% nucleated cells without HDAP, and 20.8 +/- 9.2% red blood cells. After electrophoresis, the purest fraction contained 85.8 +/- 3.5% nucleated cells with HDAP, 8.4 +/- 2.2% nucleated cells lacking HDAP, and 5.8 +/- 2.8% red blood cells; 91.9 +/- 2.4% of the nucleated cells in the purest fractions had HDAP.
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004586:Electrophoresis; D007668:Kidney / Q000166:cytology; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000201:enzymology; D051381:Rats
D000469:Alkaline Phosphatase