A new spectrophotometric cuvette holder for low temperature studies; its application to the study of carbonmonoxyhemoglobin oxidation rate.
A new spectrophotometric cuvette holder to be used for subzero temperature is described. The device is easily adaptable to a commercial spectrophotometer and it was checked down to --40 degrees C. Satisfactory mixing of the reactants contained in the cuvette at low temperatures is attained using a special stirrer and suitable solution volumes. The rate of carbonmonoxyhemoglobin oxidation by K3Fe(CN)6 at different subzero temperatures has been studied using this apparatus; the results are in agreement with the extrapolated data at room temperature.
D002263:Carboxyhemoglobin / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D010108:Oxyhemoglobins / Q000378:metabolism; D013053:Spectrophotometry / Q000295:instrumentation* / Q000379:methods; D013696:Temperature
D006454:Hemoglobins; D010108:Oxyhemoglobins; D002263:Carboxyhemoglobin