Mutants of Sindbis virus. III. Host polypeptides present in purified HR and ts103 virus particles.
The amounts of host-encoded protein present in purified Sindbis virions of both the HR strain and of a mutant (ts103) which makes multicored particles were examined. Cells were labeled with [35S]methionine before infection and with [3H]methionine postinfection. Virions were purified by velocity sedimentation and isopycnic banding, and their polypeptides were examined by polyacrylamide gels in a sodium dodecyl sulfate-containing discontinuous buffer system. Host prelabeled material was found principally in a small number of discrete polypeptides in HR virions, which contained as little as 0.2% host-encoded protein. Virus-sized particles of mutant ts103 contained significantly more host material, and multiploid particles from ts103 infection contained up to 12% host prelabeled protein.
D000818:Animals; D002642:Chick Embryo; D046508:Culture Techniques; D005347:Fibroblasts / Q000032:analysis*; D009154:Mutation; D010455:Peptides / Q000032:analysis*; D012845:Sindbis Virus / Q000032:analysis* / Q000235:genetics / Q000254:growth & development; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics
D010455:Peptides; D014764:Viral Proteins