Production of cellulases by Trichoderma reesei QM 9414 in fed-batch and continuous-flow culture with cell recycle.
The scope in improving enzyme productivities from the cellulose fermentation process is examined in laboratory-scale fermentors. The maximum productivity (30 IU/liter hr) is attained in a continuous-culture process with cell recycle using modified medium containing 0.5% cellulose. Optimum dilution rate and recycle ratio are determined as 0.025 hr-1 and 1.2, respectively, for the process. The system is analyzed and steady-state equations for predicting enzyme protein concentrations in the fermentor are developed. In fed-batch cultures, slow addition of cellulose at high concentrations can improve enzyme productivity by as much as 33% over a batch process. The scope and results of using modified medium for cellulase production are also presented.
D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose / Q000378:metabolism; D005285:Fermentation; D007700:Kinetics; D008722:Methods; D003904:Mitosporic Fungi / Q000201:enzymology*; D014242:Trichoderma / Q000166:cytology / Q000201:enzymology*
D002482:Cellulose; D002480:Cellulase