title: Histochemistry of carbohydrates in the epithelium lining the bulbourethral gland of the rat.
abstract: The histochemistry of carbohydrates has been studied in the epithelium lining the bulbourethral gland of the rat by means of light- and electron-microscopic methods. The results obtained show that the cytoplasms of the epithelial cells contain neutral and acidic carbohydrates. The neutral carbohydrates exhibited positive reactions with periodic acid-Schiff and periodic acid-thiosemicarbazide-silver proteinate, whereas the acidic carbohydrates reacted positively with alcian blue (AB; pH 1.0 and 2.5) and dialyzed iron. Most neutral carbohydrates were found to be glycoproteins localized within the secretory granules. The acidic carbohydrates consist of at least two types, AB (pH 1.0)-reactive sulfated and AB (pH 2.5)-reactive nonsulfated carbohydrates; most nonsulfated carbohydrates were determined to be sialic acid. The acidic carbohydrates were also localized within the secretory granules.
mesh_terms: D000818:Animals; D002030:Bulbourethral Glands / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000032:analysis; D004848:Epithelium / Q000032:analysis; D006003:Glycogen / Q000032:analysis; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D051381:Rats
chemical_list: D002241:Carbohydrates; D006003:Glycogen