Preparation of [32P]phosphatidylcholine and [32P]lysophosphatidylcholine by using soya beans.
This method describes a procedure that can be carried out easily to obtain large amounts of [32P]phosphatidylcholine and [32P]lysophosphatidylcholine. The method involves germinating soya beans in the presence of [32P]Pi. The yield was 0.58% for [P]phosphatidylcholine and 0.52% for [32P]lysophosphatidylcholine, and the specific radioactivity of both was 10(7) d.p.m./mumol.
D006868:Hydrolysis; D007553:Isotope Labeling / Q000379:methods*; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010761:Phosphorus Radioisotopes; D013025:Glycine max
D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010761:Phosphorus Radioisotopes