Studies on the time and localization of the puberal desensitization to oestrogen in female rats.
Immature and postpuberal female rats were ovariectomized at 20 or 27 days of age or on the day of the first vaginal oestrus and chronically implanted with oestradiol benzoate (OB) and cholesterol at the ratios of 1 : 60, 1 : 120 or 1 : 240 on the day following castration. Autopsy was performed on day 6 after implantation and the plasma LH concentration determined by radioimmunoassay. Whereas 1 : 60 and 1 : 120 implants of OB and cholesterol placed into the hypothalamic ventromedial-arcuate region depressed the castration-induced elevation of the LH level before and after puberty, the 1 : 240 mixture was effective only in immature rats, but not after vaginal opening and the first ovulation had occurred. A similar trend was recorded after implantation of OB into the cortical amygdaloid nucleus (CAN). However, the oestrogen dose had to be doubled to get comparable results. Bilateral lesioning of the CAN or deefferentation of the mediocortical amygdala by transection of the stria terminalis did not distinctively influence the LH-suppressing effect of daily s.c. injections of 0.1 or 0.05 microgram OB/100 g b. w. in prepuberal rats. The findings demonstrate a sudden change in the hypothalamic threshold to the gonadotrophin-inhibiting effect of oestrogen over a narrow range of time near the onset of puberty. They furthermore suggest that the mediocortical amygdala is not involved in possible extra-hypothalamic control of the puberal desensitization process.
D000818:Animals; D002369:Castration; D002784:Cholesterol / Q000494:pharmacology; D004343:Drug Implants; D004958:Estradiol / Q000494:pharmacology*; D004971:Estrus; D005260:Female; D007986:Luteinizing Hormone / Q000097:blood; D011247:Pregnancy; D051381:Rats; D012741:Sexual Maturation / Q000187:drug effects
D004343:Drug Implants; D004958:Estradiol; D007986:Luteinizing Hormone; D002784:Cholesterol