[Interaction of lambda bacteriophages with mammalian cells. II. Elucidation of the role of interacting components].
The study of 3H-thymidine labelled bacteriophage lambda C185757 uptake by HeLa, RH and Chinese hamster cell revealed the lack of cells or phage specificity in the phage interaction with cells. The phage uptake is shown to be an active process depending on the cell state. The mechanism of "protective" action of calcium chloride is found to be as follows: the calcium phosphate precipitate formed in phosphate-containing media absorbs the phage, thus increasing its concentration on the cell surface, which makes the pinocytosis more effective.
D000818:Animals; D001665:Binding Sites; D002122:Calcium Chloride / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology; D002460:Cell Line; D003090:Coliphages; D006224:Cricetinae; D005347:Fibroblasts; D006367:HeLa Cells; D006801:Humans; D007461:Iodoacetates / Q000494:pharmacology; D008278:Magnesium Sulfate / Q000494:pharmacology; D010873:Pinocytosis; D013696:Temperature
D002130:Calcium Phosphates; D007461:Iodoacetates; D008278:Magnesium Sulfate; D002122:Calcium Chloride
Vzaimodeĭstvie bakteriofaga lambda a kletkami mlekopitaiushchikh. Soobshchenie II. Vyiasnenie rolli vzaimodeisvuiushchikh komponentov.