title: Hexose shunt dehydrogenase activity in leukocytes isolated from bovine milk.
abstract: A spectrophotometric method for measuring small changes in light absorption at specific wavelengths from highly turbid suspensions of biological material (dual-beam spectrophotometry) has been used to measure glucose-6-phosphate oxidation and nicotinamide adenine dinucleotide phosphate (oxidized) reduction catalyzed by lysates of milk leukocytes. This reduction was correlated .84 with cellular protein concentration and, therefore, with cell concentration.
mesh_terms: D000818:Animals; D002417:Cattle; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis*; D008892:Milk / Q000166:cytology* / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology*; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis*; D013053:Spectrophotometry
chemical_list: D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase