title: Presence of a covalently linked protein on calicivirus RNA.
abstract: The infective RNA of the calicivirus, vesicular exanthema virus, has been shown to contain a protein which is apparently linked to the RNA by a covalent bond. The protein remained bound to the RNA after boiling with SDS-mercaptoethanol-urea or treating with formamide-dimethylsulphoxide but was removed by incubating with proteinase K. The mol. wt. of the protein was estimated to be about 1o X 1O(3) by electrophoresis in highly cross-linked polyacrylamide gels. The infectivity of the RNA was destroyed by removal of the protein with proteinase K.
mesh_terms: D000818:Animals; D002139:Caliciviridae / Q000032:analysis*; D002460:Cell Line; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D013552:Swine; D014720:Vesicular Exanthema of Swine / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins; D010447:Peptide Hydrolases