title: High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements.
abstract: Evidence is presented that cytoskeletal structures (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different classes of filaments or tubules can be identified both as purified protein polymers and as structured organelles within cryofractured or detergent-extracted cells.
mesh_terms: D000199:Actins; D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002645:Chickens / Q000033:anatomy & histology; D003599:Cytoskeleton / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000648:ultrastructure*; D009130:Muscle, Smooth / Q000648:ultrastructure; D009132:Muscles / Q000648:ultrastructure; D011817:Rabbits; D013552:Swine / Q000033:anatomy & histology
chemical_list: D000199:Actins