Interferon synthesis in human diploid cells pretreated with insulin.
Interferon production by Newcastle disease virus in human diploid (WI-38) cells was greatly reduced in the absence of serum. Pretreatment of the serum-free cells with insulin in excess of 1.2 IU/ml for 2 h before induction considerably increased the amount of interferon produced. No enhancement of interferon production was seen when the cells were exposed to insulin 2 h after induction or later. The insulin's effect persisted for at least 6 h after its removal, but not for 24 h. A possible mechanism of insulin action is discussed.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004171:Diploidy; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007372:Interferons / Q000096:biosynthesis*; D009522:Newcastle disease virus / Q000187:drug effects; D013997:Time Factors
D007328:Insulin; D007372:Interferons