A continuous-flow method of organ culture.
A method of perfusion organ culture is described in which explants cultured at the air-medium interface are bathed by a continuous flow of nutrient medium. Morphological studies on the fetal rat lung indicate that explant development in this system is comparable to that obtained using standard organ-culture dishes. Medium supply is easily manipulated and continuous sampling of the effluent stream is possible without disturbing the immediate explant environment. The basic design facilitates secretory-response studies on cultured organ explants as demonstrated by a study of glucose-stimulated insulin release by the neonatal rat pancreas.
D000818:Animals; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D008168:Lung / Q000196:embryology; D009924:Organ Culture Techniques / Q000295:instrumentation / Q000379:methods*; D010179:Pancreas; D051381:Rats
D003470:Culture Media; D007328:Insulin; D005947:Glucose