Androgen aromatization by luteinized bovine granulosa cells in tissue culture.
Luteinized bovine granulosa cells in tissue culture contained an active 19-hydroxylase aromatase enzyme system which converted exogenous androstenedione and testosterone to oestradiol-17beta; no oestrone was detected. In the absence of exogenous androgens, the cells failed to synthesize oestrogens due to a limited capacity to synthesize androgen precursor. Theca-lutein cells, present in those CL which synthesize oestrogens, may provide androgen precursor for aromatization by the granulosa-lutein cells.
D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D002417:Cattle; D046508:Culture Techniques; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D006107:Granulosa Cells / Q000201:enzymology*; D008184:Luteal Cells / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis*; D013250:Steroid Hydroxylases / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D013250:Steroid Hydroxylases; D001141:Aromatase