Analysis of the effectiveness of sodium chloride in dissociating non-histone chromatin proteins of cultured hepatoma cells.
We examined the ability of NaCl (at 0.15 to 3 M) to release non-histone proteins from chromatin of cultured rat hepatoma cells. The percentage of the non-histones released increased with increasing NaCl concentrations up to 0.75 M; 1 and 3 M NaCl were not significantly more effective. A maximum of 50% of the non-histone protein was recovered free of DNA. The release of non-histones from sheared and unsheared chromatin was similar. The electrophoretic patterns of the non-histone proteins released by NaCl resembled that of the non-histones released by sodium dodecyl sulfate, which indicates that many of the detectable components were at least partially released by NaCl. Some non-histones (especially low molecular weight polypeptides) were fully released by NaCl and other proteins were relatively resistant to NaCl release. Higher recoveries of NaCl-dissociated non-histones were obtained with sucrose gradient centrifugation than with centrifugation in the absence of sucrose.
D000818:Animals; D002460:Cell Line; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D008114:Liver Neoplasms, Experimental / Q000032:analysis; D046911:Macromolecular Substances; D051381:Rats; D012965:Sodium Chloride
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D046911:Macromolecular Substances; D012965:Sodium Chloride