Cyto-enetic investigations of mammals. Comparison of the genetic activity of cytostatics in mammals.
Mutagenicity tests have been performed with CP, IP and TP. There is an increase of mutagenic activity from TP to IP to CP 8 h after the second intraperitoneal application. This has been confirmed by analysis of bone marrow chromosomes of mice, rats and Chinese hamsters. The maximum mutagenic activity of all three cytostatics was found 12 h after the second injection. Thereafter the aberration rate induced by CP decreased quickly. The inactivation of IP and TP and the elimination of chromosome aberrations was delayed. Dose-effect-relationships of CP in Chinese hamsters were investigated using the micronucleaus test and analysis of bone marrow chromosomes. Correlations between the two test systems could be demonstrated. Five applications of CP led to a drastic increase of chromosome aberrations in bone marrow of Chinese hamsters. In spermatogonia, on the other hand, no significant increase was detectable. These differences are being discussed.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D003520:Cyclophosphamide / Q000494:pharmacology; D003582:Cytogenetics; D007069:Ifosfamide / Q000494:pharmacology; D008297:Male; D051379:Mice; D009154:Mutation / Q000187:drug effects*; D051381:Rats; D013045:Species Specificity; D013091:Spermatogenesis / Q000187:drug effects
D003520:Cyclophosphamide; D007069:Ifosfamide