Nondestructive distinction between aflatoxin B1 and ethoxyquin in thin-layer chromatography.
A rapid and simple method has been developed for the nondestructive distinction between aflatoxin B1 and the feed antioxidant, ethoxyquin. These two chemicals exhibit similar RF values in certain solvent systems and produce a similar bluish fluorescence under long UV (366 nm) radiation. The method involves the in situ generation of fluorescence spectra of the respective thin-layer chromatography spots. Since it is nondestructive, the method affords ancillary study of the separated aflatoxins.
D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D002642:Chick Embryo; D002855:Chromatography, Thin Layer; D005015:Ethoxyquin / Q000032:analysis*; D011804:Quinolines / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
D000348:Aflatoxins; D011804:Quinolines; D005015:Ethoxyquin