In vitro culture of growing mouse oocytes.
A method is described for in vitro culture of naked growing mouse oocytes for at least four days. Tests for metabolic function indicate that the oocytes increase in volume, accumulate glucose-6-phosphate dehydrogenase, and maintain a steady level of 3H-uridine and 3H-leucine incorporation.
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D051379:Mice; D009865:Oocytes / Q000201:enzymology / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D014529:Uridine / Q000378:metabolism
D005954:Glucosephosphate Dehydrogenase; D007930:Leucine; D014529:Uridine