5'-Terminal structure and mRNA stability.
Reovirus mRNAs with 5'terminal m7GpppGm or GpppG are more stable than mRNA containing unblocked ppG 5'-ends when injected into Xenopus laevis oocytes or incubated in cell-free protein synthesising extracts of wheat germ and mouse L cells. The greater stability of mRNA with blocked 5' termini is not dependent upon translation but seems to result from protection against 5'-exonucleolytic degradation.
D000818:Animals; D001483:Base Sequence; D002474:Cell-Free System; D005092:Exonucleases / Q000378:metabolism; D005260:Female; D006150:Guanine Nucleotides / Q000378:metabolism; D007739:L Cells / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism; D008970:Molecular Weight; D009865:Oocytes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D012087:Reoviridae; D012156:Reticulocytes / Q000378:metabolism; D013329:Structure-Activity Relationship; D014908:Triticum; D014981:Xenopus
D006150:Guanine Nucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D008780:Methyltransferases; D005092:Exonucleases