The mutagenic assay of some hair dye components, using the thymidine kinase locus of L5178Y mouse lymphoma cells.
Five hair dye components were tested for their mutagenicity at the TK +/- locus of L5178Y cells. Three of the components, m-phenylenediamine, 2 nitro-p-phenylenediamine, and 4-nitro-o-phenylenediamine, gave a positive response that was dose related; 2,4-diaminoanisole gave a questionable response; and 2,5-diaminoanisole gave a negative response. The assay was carried out in vitro without metabolic activation during a 24-hr chemical exposure period. It is concluded that further in vivo tests are necessary to establish the safety of the hair dyes.
D000818:Animals; D000840:Anisoles / Q000494:pharmacology; D002874:Chromosome Mapping; D003358:Cosmetics / Q000494:pharmacology*; D006202:Hair Dyes / Q000494:pharmacology*; D007739:L Cells; D051379:Mice; D009153:Mutagens; D010655:Phenylenediamines / Q000494:pharmacology; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism
D000840:Anisoles; D003358:Cosmetics; D006202:Hair Dyes; D009153:Mutagens; D010655:Phenylenediamines; D013937:Thymidine Kinase