[Technical problems in platelet-concentrate preparation and their clinical significance].
To obtain platelet concentrates the following procedure is in routine use in the German Red Cross Blood Bank in Ulm: 1. Harvesting of donor blood in a double bag system; 2. two step centrifugation of a) whole blood, and b) platelet rich plasma at room temperature (22 degrees C) at 300 X g for 20 min, and 1200 X g for 30 min respectively; 3, resuspension of platelets in a minimum of 50 ml of plasma. Platelet yield averages 60%. The white cell content is less than 5% as compared to the original material. Although red cells are nearly absent the ABO blood group system and the D antigen are considered for compatibility. Platelet concentrates are stores up to 72 hours at 22 degrees C at constant slow rotation. Technical and biological variables affecting platelet yield and viability are discussed in some detail.
D000017:ABO Blood-Group System; D001792:Blood Platelets / Q000502:physiology; D001793:Blood Preservation; D001803:Blood Transfusion / Q000379:methods*; D002498:Centrifugation; D006801:Humans; D010974:Platelet Aggregation; D017713:Platelet Transfusion; D013696:Temperature; D014184:Transplantation, Homologous
D000017:ABO Blood-Group System
Technische Probleme der Thrombozytenkonzentratherstellung und ihre klinische Bedeutung.