title: Secondary structure of histones and DNA in chromatin.
abstract: Laser Raman spectroscopy indicates that the inner histones which are bound to DNA in chromatin or in isolated nu bodies are similar in conformation to the inner histones which are dissociated from DNA in high-salt solutions. This structure contains, on the average, 51+/-5% alpha-helix and no substantial beta-sheet conformation. It is proposed that the protein core of the nu body has a high alpha-helix content.
mesh_terms: D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D011485:Protein Binding; D011487:Protein Conformation; D013059:Spectrum Analysis, Raman; D013696:Temperature
chemical_list: D002843:Chromatin; D006657:Histones; D004247:DNA