Chemical modification studies on the D-galactopyranosyl binding lectin from the mistletoe Viscum album L.
The role of amino, sulfhydryl, disulfide, carboxyl, phenolic, imidazole and indole groups on the agglutination of human erythrocytes by the lectin from Viscum album has been determined using specific chemical modification techniques. The results indicate that tyrosine residues participate in the hemagglutination reaction. Subunits of the lectin possess only reduced hemagglutinating ability.
D001894:Borohydrides / Q000494:pharmacology; D001974:Bromosuccinimide / Q000494:pharmacology; D002234:Carbodiimides / Q000494:pharmacology; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D005557:Formaldehyde / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology*; D008027:Light; D014784:Mistletoe / Q000032:analysis*; D037121:Plant Lectins; D010946:Plants, Medicinal; D013774:Tetranitromethane / Q000494:pharmacology
D001894:Borohydrides; D002234:Carbodiimides; D007093:Imidazoles; D037102:Lectins; D037121:Plant Lectins; D005557:Formaldehyde; D004228:Dithionitrobenzoic Acid; D013774:Tetranitromethane; D001974:Bromosuccinimide; D004047:Diethyl Pyrocarbonate