title: Possible involvement of putrescine in nucleolar formation in early embryos.
abstract: Continuous treatment of developing eggs of the polychete Ophryotrocha labronica with alpha-methylornithine, which inhibits synthesis of putrescine, led to arrest of development at gastrulation. The present ultrastructural analysis suggests that the arrest of development is due to failure to form nuclei, and thus reveals a possible role for putrescine in nucleolar formation. Further support for this contention was provided by means of electron-microscopical autoradiography. It was found that newly synthesized putrescine, derived from administered 3H-ornithine, labeled the nucleoli intensely at the time of their normal appearance during gastrulation, the time at which the rate of endogenous putrescine synthesis is maximal. These observations have led to the conclusion that putrescine synthesis may be directly involved in formation of nucleoli.
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D009952:Ornithine / Q000031:analogs & derivatives / Q000494:pharmacology; D010063:Ovum / Q000187:drug effects / Q000254:growth & development* / Q000648:ultrastructure; D011077:Polychaeta / Q000196:embryology*; D011700:Putrescine / Q000096:biosynthesis*
chemical_list: D009952:Ornithine; D011700:Putrescine