title: A model for vesicular exanthema virus, the prototype of the calicivirus group.
abstract: The structure of vesicular exanthema virus, the prototype member of the calicivirus group, has been studied in more detail. The RNA comprises 18% of mol. wt. of about 2.8 x 10(6), based on polyacrylamide gel electrophoresis experiments in the presence of formaldehyde. The virus contains one major polypeptide, mol. wt. 70 x 10(3) as determined by polyacrylamide gel electrophoresis and by chromatography on Sepharose 6B in the presence of 6 M-guanidine. Further evidence for the presence of a single major polypeptide was obtained by tryptic peptide analysis of 35S-methionine labelled virus. The mol. wt. of a protein oligomer produced by adjusting the pH of virus suspensions to 3.5 was c. 200 x 10(3). On the basis of these data we propose a T = 3 model for the virus capsid incorporating 180 copies of the virus protein.
mesh_terms: D000818:Animals; D002139:Caliciviridae / Q000648:ultrastructure*; D008961:Models, Structural; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D013552:Swine; D014720:Vesicular Exanthema of Swine / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis
chemical_list: D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins