Incorporation of galactose into galactosyltransferase.
Bovine skim milk galactosyltransferase (EC 2.4.1.22) retained its catalytic activity after partial enzymatic removal of sialic acid and galactose. Desialylated and degalactosylated galactosyltransferase was a galactosyl acceptor in the galactosyltransferase reaction. [14C]Galactose from UDP-[14C]galactose was incorporated into the carbohydrate-depleted galactosyltransferase by native galactosyltransferase. The results suggest that galactosyltransferase participates in the biosynthesis of its glycopeptides of the sialic acid-galactose-N-acetylglucosamine type.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D005690:Galactose / Q000378:metabolism*; D005700:Galactosyltransferases / Q000096:biosynthesis / Q000378:metabolism*; D006020:Glycopeptides / Q000096:biosynthesis; D008892:Milk / Q000201:enzymology*; D012794:Sialic Acids
D006020:Glycopeptides; D012794:Sialic Acids; D005700:Galactosyltransferases; D005690:Galactose