Neurochemical and behavioural effects of extended exposure to isopropanol vapour with simultaneous ethanol intake.
Exposure of male Wistar rats to 12.3 mumol/l (300 ppm) isopropanol vapour for 5-21 weeks, 5 days a week for 6 h daily with a simultaneous ethanol administration in drinking water (5% v/v) caused a significant increase in isopropanol removal as assessed by blood isopropanol and acetone determinations. Ethanol treatment caused a marked synergistic effect during early exposure. Neurochemical studies revealed decreased superoxide dismutase and azoreductase activities at the end of the exposure whereas increased protein degradation was found in glial cells isolated from ethanol-fed rats throughout the experiment. Analyses of spinal cord axon lipid composition showed increases in cholesterol content in relation to lipid phosphorus in animals exposed to isopropanol or to the isopropanol and ethanol combination. Behavioural tests indicated minor effects on emotional reactivity from the 10th week onwards with isopropanol exposure whereas caffeine-stimulated activity was augmented only in rats ingesting ethanol. Co-exposure to isopropanol vapour abolished the increased excitability. The data indicate that marked metabolic and functional adaptation towards the small-molecular-weight alcohols takes place at moderate dose levels.
D000433:1-Propanol / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity*; D000096:Acetone / Q000097:blood; D000818:Animals; D001369:Axons / Q000378:metabolism; D001391:Azo Compounds / Q000032:analysis; D001522:Behavior, Animal / Q000187:drug effects*; D002531:Cerebellum / Q000201:enzymology; D002784:Cholesterol / Q000032:analysis; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis; D009252:NADPH Dehydrogenase / Q000032:analysis; D009601:Nitroreductases; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism; D013482:Superoxide Dismutase / Q000032:analysis
D001391:Azo Compounds; D000096:Acetone; D000431:Ethanol; D000433:1-Propanol; D002784:Cholesterol; D013482:Superoxide Dismutase; D009247:NADH, NADPH Oxidoreductases; D009252:NADPH Dehydrogenase; D009601:Nitroreductases; C021106:azoreductase