title: Biological and biophysical characteristics of mouse adenovirus, strain FL.
abstract: Mouse adenovirus, strain FL (MAV), is best propagated in primary rather than secondary cultures of mouse kidney cells. Virus release from the cells into the medium is fairly efficient; the average yield per cell is about 1000 TCID50. Morphological and other biophysical characteristics are those of a typical adenovirus: the icosahedral shell with a diameter of 74 nm, fiber projections of 29 nm length, its localization inside the infected kidney cells, a buoyant density in CsC1 of 1.34 g/ml, inhibition of multiplication by inhibitors of DNA synthesis. The thermostability is higher than that of human adenoviruses, whereas, in contrast to these, MAV is inactivated to a great extent by trypsin. Complete or incomplete hemagglutinin or toxin-like activity were not detected. The virus shows no cross-neutralization with human adenoviruses and a one-side cross-reaction with another murine adenovirus strain K-87. Soluble complement-fixing antigen of MAX exhibits a sedimentation rate of 12S identical to the hexon component of human adenoviruses; both antigens show a partial antigenic relationship in tests with appropriate antisera.
mesh_terms: D000256:Adenoviridae / Q000254:growth & development / Q000276:immunology / Q000648:ultrastructure; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002213:Capsid; D002460:Cell Line; D003429:Cross Reactions; D046508:Culture Techniques; D003561:Cytarabine / Q000494:pharmacology; D006358:Hot Temperature; D006918:Hydroxyurea / Q000494:pharmacology; D007065:Idoxuridine / Q000494:pharmacology; D007668:Kidney; D051379:Mice; D014357:Trypsin / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000956:Antigens, Viral; D003561:Cytarabine; D014357:Trypsin; D007065:Idoxuridine; D006918:Hydroxyurea