Effect of pyridoxine deficiency on cholesterogenesis in rats fed different levels of protein.
Hepatic cholesterol contents in rats fed a 70% or 20% casein diet with or without pyridoxine was determined. In the case of the 70% casein group, pyridoxine-deficient rats showed a higher content than the control. The increment was mainly due to the accumulation of an ester form of the cholesterol. On the other hand, pyridoxine-deficient rats in the 20% casein group showed a slightly lower content. The cholesterol content in liver microsomal fractions was lower in the 20% casein pyridoxine-deficient group and serum cholesterol level was lower in the 70%-casein pyridoxine-deficient group than those in respective control groups. Incorporation of [14C]acetate into cholesterol was studied using liver slices, and significant stimulation was observed in pyridoxine-deficient rat fed a 20% or 70% casein diet. There was no difference in intestinal cholesterogenesis between the control and the deficient groups.
D000818:Animals; D002784:Cholesterol / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D002940:Circadian Rhythm; D046508:Culture Techniques; D004044:Dietary Proteins / Q000008:administration & dosage*; D005215:Fasting; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000032:analysis / Q000378:metabolism; D008297:Male; D011736:Pyridoxine / Q000378:metabolism*; D051381:Rats; D026681:Vitamin B 6 Deficiency
D004044:Dietary Proteins; D002784:Cholesterol; D011736:Pyridoxine