Failure to separate human x- and y-chromosome bearing spermatozoa by Sephadex gel-filtration.
Human spermatozoa has been filtered on Sephadex gel columns (G 50 fine powder). 50--70% of the spermatozoa put on the gel bed had been recovered within the filtrate, the remaining spermatozoa--not absorbed to gel particles--were found in the upper third of the column. The percentage of motile spermatozoa was higher (80--95%) in the first fraction as compared to the control samples (40--65%). This technique seems to be unsuitable for a successful separation of human x- and y-spermatozoa, an enrichment of x-spermatozoa couldn't be obtained within the first fractions of the filtrate.
D002469:Cell Separation / Q000379:methods; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D008297:Male; D012730:Sex Chromosomes; D013081:Sperm Motility; D013094:Spermatozoa / Q000648:ultrastructure*; D014960:X Chromosome; D014998:Y Chromosome