Yellow mutations alter chloroplast ribosomal proteins in Chlamydomonas reinhardii.
Ribosomes and ribosomal proteins from wild-type and three yellow mutants of Chlamydomonas reinhardii were analyzed and compared by two-dimensional gel-electrophoresis. Mixotrophycally grown mutants differ from wild-type cells in lowered chlorophyll content. Analytical ultracentrifugation analysis of cell extracts showed a reduced amount of 70S ribosomes and an increased level of 50S subunits in mutants y-27 and y-28. Two-dimensional gel electrophoresis indicated considerable alterations in the protein composition of 70S ribosomes of the mutants. Two proteins of 70S ribosomes were altered in the y-27 and y-28 mutants. Two proteins were absent from the electrophoretograms of the yellow-76 mutant and seven proteins were present in lowered concentrations. The mutations are inherited in a non-Mendelian manner. The protein alterations in 70S ribosome are most probably localized in the chloroplast DNA.
D002696:Chlamydomonas / Q000032:analysis*; D002734:Chlorophyll / Q000032:analysis; D002736:Chloroplasts / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009154:Mutation; D012269:Ribosomal Proteins / Q000032:analysis*; D012270:Ribosomes / Q000032:analysis
D012269:Ribosomal Proteins; D002734:Chlorophyll