title: Bile acids. LIX. Purification of 5 alpha-anhydrocyprinol by preparative high-performance liquid chromatography.
abstract: 26,27-Oxido-5 alpha-cholestane-3 alpha,7 alpha,12 alpha-triol can be obtained in a homogeneous state in gram quantities by passing it through one PrepPak-500/Silica cartridge mounted in a Waters Assoc. preparative liquid chromatograph. The elution solvent was methanol-chloroform (1:14). The isolated material was analyzed for purity by several chromatographic means and by elemental analysis, and was finally characterized by the usual spectroscopic means. Gas-liquid chromatography of its trimethylsilyl ether indicated the formation of a tetrakis-trimethylsilyl-26-chloro derivative, in addition to the expected tris-trimethylsilylated substance. The structure of the former compound is deduced from the fragmentation and isotope abundance in its mass spectrum and from chemical principles.
mesh_terms: D000818:Animals; D001646:Bile / Q000032:analysis; D002347:Carps; D002777:Cholestanols / Q000302:isolation & purification*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry
chemical_list: D002777:Cholestanols; C023378:anhydrocyprinol