[Effect of temperature on the duration of mitosis in mammalian cells cultivated outside the body].
Nine cell strains of different origin were cultivated at 28--36 degrees with the interval of 2 degrees. During the phase of logarithmic culture growth, the duration of mitosis (Tm) was determined by means of colchicine method. A strict temperatural dependence Tm, obeyed to Arrenius' law was revealed. Temperature range within which Arreinius' law is valid in different cell strains is not alike. Cultivation of L cells and connective tissue cells from Chinese hamster to 39, 41and 42 degrees demonstrated their upper critical point Tm to be for L cells 39 degrees, for connective tissue cells from the Chinese hamster--41 degrees. Electron microscopic investigations demonstrated that cell cultivation within physiological (mitosis destroying) range of temperatures does not notably effect their ultrastructural organization.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D006801:Humans; D007739:L Cells; D008322:Mammals; D008938:Mitosis; D013696:Temperature; D013997:Time Factors
Vliianie temperatury na prodolzhitel'nost' mitoza kletok mlekopitaiushchickh, kul'tivireumykh vne organizma.