The use of ultrasonication to remove non-specific precipitate.
The non-specific artifactual precipitate found in glycol methacrylate (GMA) embedded tissues, following acid phosphatase histochemical incubation, can now be eliminated with brief ultrasonication. A treatment of 1 to 2 min totally eliminated the artifactual residue, leaving the true histochemical reaction product and the physical integrity of the tissues intact. With this technique, most loosely bound precipitates, which clutter and mask GMA or conventionally embedded tissues, may now be treated and eliminated.
D000135:Acid Phosphatase / Q000302:isolation & purification*; D000818:Animals; D002642:Chick Embryo; D006651:Histocytochemistry; D007678:Kidney Glomerulus / Q000201:enzymology*; D011102:Polyhydroxyethyl Methacrylate; D013010:Sonication; D014465:Ultrasonics
D011102:Polyhydroxyethyl Methacrylate; D000135:Acid Phosphatase