Antiviral and interferon-inducing activities of a new peptidomannan, KS-2, extracted from culture mycelia of Lentinus edodes.
Oral (PO) administration of KS-2 to adult DDI mice resulted in a peak serum interferon (IF) titer of 800 units (U)/ml 20 hours after administration with detectable levels persisting until 30 hours. After intraperitoneal (IP) injection, a peak serum IF titer of 1,600 U/ml was detected and it followed the same time course as that of oral administration. The IF induced by KS-2 shared certain physico-chemical properties with the standard preparation of immune IF and was not neutralized by an antiserum against type I IF. In mice infected intranasally (IN) with influenza A2 (H2N2) virus, KS-2 was found to possess significant protective activities. Efficacy of the agent was evidenced by an increase in survivor number, a prolongation of mean survival time, an inhibition of the development of lung consolidation induced by the viral infection and a decrease in virus titer in lung tissues. Both PO and IP administrations of KS-2 protected mice against infection and significant antiviral activities were achieved not only by prophylactic but also chemotherapeutic administration. The protective activities of KS-2 against influenza virus infection in mice are discussed in view of the immunopotentiation of the host animals.
D000363:Agaricales / Q000378:metabolism*; D000818:Animals; D000998:Antiviral Agents; D005260:Female; D006020:Glycopeptides / Q000302:isolation & purification / Q000494:pharmacology; D007369:Interferon Inducers; D008168:Lung / Q000473:pathology; D008297:Male; D008351:Mannans / Q000627:therapeutic use; D051379:Mice; D008815:Mice, Inbred Strains; D009976:Orthomyxoviridae Infections / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control; D011509:Proteoglycans; D013997:Time Factors
D000998:Antiviral Agents; D006020:Glycopeptides; D007369:Interferon Inducers; D008351:Mannans; D011509:Proteoglycans; C010770:polysaccharide-K