Localisation of foldback DNA sequences in nuclei chromosomes of Scilla, Secale, and of mouse.
Foldback DNA, prepared from mouse and Scilla sibirica main band DNA, and from rye (Secale cereale) total DNA, was characterised by denaturation, renaturation, and electron microscopy. 3H-cRNA of this DNA was hybridised in situ to nuclei and chromosomes of the respective species. There is no universal labelling pattern among the three species. In mouse, highly repetitive foldback DNA is present in the whole chromatin including the satellite DNA-containing regions. In Scilla sibirica, on the contrary, the highly repetitive foldback sequences are excluded form the satellite DNA loci and are arranged in clusters in the remaining chromatin. In rye, there is a clear preferential labelling of the chromocenters in the interphase nuclei as well as metaphase chromosomes, indicating that highly repetitive foldback DNA is preferentially located among other highly repetitive sequences.
D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000648:ultrastructure; D004247:DNA / Q000235:genetics*; D007700:Kinetics; D051379:Mice / Q000235:genetics*; D009695:Nucleic Acid Renaturation; D010944:Plants / Q000235:genetics*; D010946:Plants, Medicinal / Q000235:genetics*
D004247:DNA