[On the physiology of growth and riboflavin overproduction of Eremothecium ashbyii. III. Investigations on the incorporation of radioactive labeled substrates in cell material and riboflavin (author's transl)].
The incorporation of glycine-2-14C and adenine-U-14C in cell material and riboflavin was investigated in order to determine the proportions metabolites were channeled in growth processes and product synthesis of Eremothecium ashbyii. Extraction- and measurement methods were developed to compare the incorporation of radioactive metabolites into different cell fractions. Young cells incorporate the labeled compounds in a high rate into the cell material. With increasing age of the culture the incorporation of radioactivity is more directed to riboflavin. 96 hours old cells incorporate about seven times more 14C-adenine into riboflavin than into the cell material.
D000225:Adenine / Q000378:metabolism; D001203:Ascomycota / Q000378:metabolism*; D005656:Fungal Proteins / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D009696:Nucleic Acids / Q000096:biosynthesis; D012256:Riboflavin / Q000096:biosynthesis*; D004718:Saccharomycetales / Q000254:growth & development / Q000378:metabolism*
D005656:Fungal Proteins; D009696:Nucleic Acids; D005947:Glucose; D000225:Adenine; D005998:Glycine; D012256:Riboflavin
Zur Physiologie des Wachstums und der Riboflavin-Uberproduktion von Eremothecium ashbyii. III. Untersuchungen über den Einbau von radioaktiven Substraten in Zellsubstanz und Riboflavin.