Cloning of soybean leghemoglobin structural gene sequences synthesized in vitro.
Double-stranded soybean leghemoglobin DNA was synthesized from leghemoglobin mRNA isolated from soybean nodules. The dsDNA was inserted into the Bam H1 site of plasmid pBR322 using the poly-dAT-joiner method. A cloned DNA fragment of one recombinant plasmid was isolated and characterized by restriction endonuclease digestion. The restriction cleavage map and the DNA sequence of a selected part of the inserted DNA are in complete accordance with the amino-acid sequence of soybean leghemoglobin.
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000096:biosynthesis; D005796:Genes; D006420:Hemeproteins / Q000096:biosynthesis*; D007874:Leghemoglobin / Q000096:biosynthesis*; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000378:metabolism*; D010957:Plasmids; D013025:Glycine max
D004274:DNA, Recombinant; D006420:Hemeproteins; D007874:Leghemoglobin; D004262:DNA Restriction Enzymes