The interference of endogenous antibodies to bovine lactalbumin in the radioimmunoassay of human lactalbumin in serum.
Using a radioimmunoassay for the milk protein lactalbumin in human serum, we have found that falsely high results can be caused by cross-reacting human antibodies to bovine lactalbumin. A reliable method of circumventing this problem is described. When such interference had been eliminated, circulating lactalbumin was not detected in normal men or post-menopausal women, but could still be found in a proportion of non-pregnant, non-lactating pre-menopausal women.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000906:Antibodies; D002417:Cattle / Q000276:immunology; D003429:Cross Reactions; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007768:Lactalbumin / Q000097:blood* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay / Q000379:methods
D000906:Antibodies; D007768:Lactalbumin