title: An in vitro study of the exposure of mixed populations of normal oropharyngeal bacteria to cigarette smoke.
abstract: Mixed populations of freshly isolated oropharyngeal bacteria growing in dextrose broth at 37 degrees C were subjected to smoke from four cigarettes over a 3 h period. Each cigarette, a commercially available brand, contained 23.0 mg of tar and 1.4 mg of nicotine. At hourly intervals eight puffs of smoke from a cigarette were passed over a 1.0 ml suspension of bacteria dispersed in a thin layer in a sterile tissue culture flask. Normal filtered air was in contact with the suspension between smoke treatments. Exposure to cigarette smoke resulted in a marked decrease in numbers of viable bacteria. Non-filter cigarette smoke had a greater detrimental effect than smoke from filter-tipped cigarettes. The predominant bacteria, non-haemolytic streptococci, and less numerous bacteria such as staphylocci, were equally sensitive to the toxic action of cigarette smoke.
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D006801:Humans; D009960:Oropharynx / Q000382:microbiology*; D010947:Plants, Toxic; D012906:Smoke; D013210:Staphylococcus / Q000254:growth & development; D013291:Streptococcus / Q000254:growth & development; D014026:Nicotiana
chemical_list: D012906:Smoke