Fluorography of tritium-labelled proteins in immunoelectrophoresis.
A method is described for detecting 3H-labelled proteins in immunoelectrophoretic systems performed on agarose gels. The method is based on the incorporation of a polyacrylamide gel into the agarose gel after the electrophoresis. This mixed gel has the characteristics of a polyacrylamide gel, making it possible to use fluorography as has been described for polyacrylamide gels. The applicability of the fluorography method is demonstrated by analyzing 3H-labelled human serum albumin and 3H-labelled pig intestinal brush border proteins by quantitative immunoelectrophoresis.
D000818:Animals; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005453:Fluorescence; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007422:Intestines / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D008871:Microvilli / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis; D013552:Swine; D014316:Tritium
D008565:Membrane Proteins; D011506:Proteins; D012709:Serum Albumin; D014316:Tritium