Factors in the survival of stored corneas.
In studying the survival of stored corneas we wished to know if excising the cornea was the most critical step or if one storage medium was superior to another. Rabbit corneas were excised and stored in various media at 4 degrees C or 23 degrees C for four days. The storage media included M-K medium, balanced salt solution (BSS) and 10(-8)M concentration of hydrocortisone in M-K medium and BSS. Comparisons were made by measuring acid phosphatase as an index of autolysis. Results showed a reduced amount of autolysis at 4 degrees C as compared to 23 degrees C. No statistical difference between M-K medium and BSS was seen but steroid decreased autolysis. M-K medium plus hydrocortisone seemed to be the best of the solutions studied, but removal of the cornea seemed to be the most important factor in its survival.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001329:Autolysis; D003315:Cornea / Q000187:drug effects; D003470:Culture Media; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007552:Isotonic Solutions; D008297:Male; D009926:Organ Preservation / Q000379:methods; D009930:Organic Chemicals; D011817:Rabbits; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods; D014022:Tissue Survival / Q000187:drug effects
D003470:Culture Media; D007552:Isotonic Solutions; C015368:McCarey-Kaufman medium; D009930:Organic Chemicals; D000135:Acid Phosphatase; D006854:Hydrocortisone