Somatic cell genetic assignment of the human gene for mitochondrial NADP-linked isocitrate dehydrogenase to the long arm of chromosome 15.
A double-immunodiffusion method has been developed to detect human mitochondrial NADP-linked isocitrate dehydrogenase (EC 1.1.1.42; designated as IDH-2) using rabbit antiserum against the relevant enzyme. The method allows one to distinguish human IDH-2 from its mouse counterpart in extracts from human-mouse somatic cell hybrids. A correlation was found between the expression of human IDH-2 and the presence of human chromosome 15 in a "panel" of eight independent hybrid clones. Analysis of human marker enzymes for 37 different clones revealed a syntenic relationship between IDH-2 and mannose phosphate isomerase (EC 5.3.1.8; MPI), which has been assigned to chromosome 15 (1). These results permit the assignment of the structural gene for human IDH-2 to human chromosome 15. IDH-2 and human cytoplasmic IDH (IDH-1) were found to be asyntenic. Evidence from hybrid clones carrying a human X/15 translocation chromosome indicates that the human IDH-2 gene can be localized to the q11-qter region of chromosome 15.
D000818:Animals; D002874:Chromosome Mapping; D002901:Chromosomes, Human, 13-15; D002999:Clone Cells; D005260:Female; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D007521:Isocitrate Dehydrogenase / Q000235:genetics*; D051379:Mice; D014178:Translocation, Genetic; D014960:X Chromosome
D007521:Isocitrate Dehydrogenase