Immunologic and physiologic responses of calves inoculated with potassium thiocyanate extract of Pasteurella multocida type A.
Inoculation of calves with potassium thiocyanate (KSCN) extract of Pasteurella multocida type A in saline-tris buffer or in Freund's incomplete adjuvant or modified Freund's incomplete adjuvant resulted in the elicitation of agglutinating, hemagglutinating, bactericidal and homocytotropic antibodies. The antibody response was significantly (P = 0.05) higher in calves inoculated with the KSCN extract in either adjuvant than in those inoculated with the extract in saline-tris buffer. Hemagglutination also was observed if the KSCN extract of P hemolytica was used for sensitizing the tanned sheep red blood cells. Further, calf anti-P multocida extract antisera also was bactericidal to P hemolytica. The KSCN extract of P multocida was found to be nontoxic to calves at 2 doses tested, as judged by an evaluation of total and differential leukocyte counts, body temperature, and pulse rates at various intervals after inoculation.
D000372:Agglutination Tests; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001770:Blood Bactericidal Activity; D002417:Cattle / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D010325:Pasteurella / Q000276:immunology*; D011650:Pulmonary Alveoli / Q000502:physiology; D013861:Thiocyanates
D000942:Antigens, Bacterial; D013861:Thiocyanates