Gangliosides of cultured cells of a rat mammary carcinoma cell line.
Ganglioside content of rat mammary carcinoma-derived cells grown in layers in vitro was nearly as high as that of apical membrane-derived milk fat globule membrane and nearly four time higher than the content of normal, lactating mammary tissue on a protein basis. The major ganglioside of these carcinoma-derived cells was identified as GDla (sialic acid-Gal-GalNAc-sialic acid)-Gal-Glc-Cer. Relative to carcinoma-derived cells, rat mammary tissue and milk fat globule membrane had more complex ganglioside patterns but appeared to lack substantial quantities of GDla.
D000818:Animals; D002460:Cell Line; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000032:analysis*; D008325:Mammary Neoplasms, Experimental / Q000032:analysis*
D005732:Gangliosides