In vitro characteristics of two established hepatoma cell lines.
The in vitro behaviours of a fibroblast-like (DENA-RH 13) and an epithelial-like (DENA-RH 13A) cell line, derived from a chemically induced hepatocarcinoma of rat are described. The DENA-RH 13A can be maintained and studied either in monolayer as a transplantable solid tumor or in fluid-suspension culture as ascites tumor in Wistar rats. The cytomorphology and growth pattern of the clones of the cell lines are described. While DENA-RH 13 cells produced undifferentiated tumors with mainly sarcoma-like structures in allogeneic hosts the epithelial-like cell line (DENA-RH 13A) grew in a carcinoma-like pattern in animals.
D000818:Animals; D002460:Cell Line; D004052:Diethylnitrosamine; D004848:Epithelium / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000473:pathology*
D004052:Diethylnitrosamine