Effect of interferon on dimethyl sulfoxide-stimulated Friend erythroleukemic cells: ultrastructural and biochemical study.
Treatment of dimethyl sulfoxide-stimulated Friend erythroleukemic cells (clone 745) with mouse interferon (50 U/ml) led to the following changes: (i) a net decrease (40 to 60%) in both the total number of apparently newly synthesized virion particles per cell section and in the average number of cell sections containing one or more virion particles; (ii) a large decrease (80 to 90%) in the number of particles released into the supernatant fluid, as assayed by reverse transcriptase activity; (iii) an initial increase in the number of "immature" or "enveloped A-type" virions followed by an increase in the accumulation of empty, core shell-like particles; and (iv) a decrease in the number of cytoplasmic vacuolar structures, which have been implicated as a major site of virus production and which we show here by serial sectioning to be, in several instances, invaginations of the plasma membrane. The effects on virus production were noticeable 2 h after interferon addition and reached their full extent by 13 h. We conclude from these observations that interferon acts upon the late stage(s) of virion maturation, leading both to a decrease in virion production as well as to the formation of defective particles. In contrast, a small but significant increase in the rate at which globin mRNA and hemoglobin accumulate is observed after interferon treatment.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005622:Friend murine leukemia virus / Q000254:growth & development* / Q000648:ultrastructure; D005914:Globins / Q000096:biosynthesis; D006454:Hemoglobins / Q000096:biosynthesis*; D007372:Interferons / Q000494:pharmacology*; D007942:Leukemia, Experimental; D008954:Models, Biological; D009024:Morphogenesis; D014779:Virus Replication
D006454:Hemoglobins; D005914:Globins; D007372:Interferons; D004121:Dimethyl Sulfoxide