title: Two immunologically distinct human acidic beta-galactosidase A isozymes.
abstract: Two acidic beta-galactosidase isozymes (designated A1 and A2) were separated by isoelectric focusing from beta-galactosidase A of human liver. Kinetic studies with 4-methylumbelliferyl-beta-D-galactopyranoside substrate revealed similar parameters for both. The Km value was 0.32 mmol/1 for A1 and 0.30 mmol/1 for A2 and Vmax values of 59.3 and mumol min-1 mg-1, respectively. The pH optimum was 4.2 for beta-galactosidase A1 and 4.5 for the A2 form. The A1 enzyme form was shown to be more heat labile than the A2. Significant differences were observed with antibody preparations against the two enzyme forms. Using the anti-A1 antibodies two precipitin arcs with residual enzymatic activity were obtained by immunoelectrophoresis of beta-galactosidase A whereas only one with anti-A2 antibodies. Anti-A1 precipated 85% of the original activity present in beta-galactosidase A and only 56% could be precipated by anti-A2. The possibility of common structural components is suggested.
mesh_terms: D000818:Animals; D005696:Galactosidases / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007118:Immunoassay; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology
chemical_list: D007527:Isoenzymes; D005696:Galactosidases