Action of ultrasonic irradiation on the DNA of cultivated bovine epithelial lens cell.
Cultivated epithelial lens cells have been submitted to a 20 kHz continuous ultrasonic irradiation. At relativity high intensity, destruction of the cells was observed. At lower intensities, where no cell destruction appeared, the molecular weight of the single strand DNA of these cells was monitored to determine whether breakage of DNA molecules was induced by the ultrasound. No breakage of the single strand DNA was observed for intensities below 0.1 and 0.4 W cm-2 with irradiation times of less than 20 minutes and 15 seconds, respectively. These intensities and their corresponding irradiation time are higher than those used in current diagnostic practice.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004247:DNA; D004847:Epithelial Cells; D007908:Lens, Crystalline / Q000166:cytology; D008970:Molecular Weight; D014465:Ultrasonics
D004247:DNA