Trypsin treatment which elicits DNA synthesis, removes a high molecular weight glycoprotein from the plasma membrane of sea urchin embryonic cells.
SDS-polyacrylamide gel electrophoresis of plasma membrane proteins from sea urchin embryos at the swimming blastula stage allows the identification of fifteen-seventeen components, five of which are glycoproteins. One of these (molecular weight about 145,000) is almost completely absent in the plasma membrane of trypsin treated cells.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D010063:Ovum / Q000187:drug effects* / Q000378:metabolism; D012617:Sea Urchins; D014357:Trypsin / Q000494:pharmacology*
D006023:Glycoproteins; D008565:Membrane Proteins; D004247:DNA; D014357:Trypsin