A new rapid rosette-forming cell micromethod for the detection of antibody-synthesizing hybridomas.
The method reported here describes a microrosetting assay which allows the early detection of antibody-synthesizing hybridomas. This method is specific, requires very few hybridoma cells and avoids artifacts resulting from the presence of antibodies secreted by spleen cells in the primary hybridoma cultures. It is easy to perform and allows the screening of more than one hundred independent clones within a few hours.
D000818:Animals; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology*; D001666:Binding Sites, Antibody; D002459:Cell Fusion; D005362:Ficoll / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000276:immunology*; D012397:Rosette Formation; D012756:Sheep; D013997:Time Factors; D014301:Trinitrobenzenes / Q000276:immunology
D014301:Trinitrobenzenes; D005362:Ficoll