Methadone induced lysis of mammalian cells.
Methadone induced lysis of human erythrocytes and mouse leukemic cells was studied. The cells lyse without prior swelling that is a necessary step of colloid osmotic lysis. Methadone is accumulated by both cell types, and is widely distributed intracellurly in mouse leukemic cells. The maximum lytic rate is roughly proportional to the amount of methadone uptake and the Q10 for lysis is equal to the Q10 for methadone partitioning between octanol and water. It is concluded that the cells lyse as a result of a non-specific disruption of the plasma membrane.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008691:Methadone / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D009996:Osmotic Fragility / Q000187:drug effects; D013347:Subcellular Fractions / Q000378:metabolism
D008691:Methadone