title: [Lipolytic activity from milk isolated strains of the species Thermoactinomyces vulgaris, Tsiklinsky 1899 (author's transl)].
abstract: Thirty strains of the species Thermoactinomyces vulgaris (Tsiklinsky, 1988) were isolated from milk and tested for their lipolythic activity. After incubation for 120 hours at 55 degrees C they formed distinct diffusion zones on culture media containing tributyrin and tween-80. In all strains exocellular lipases were found. Their action on the milk fat increased the number of the free fatty acids. Triglycerides, free fatty acids as well as diglycerides and monoglycerides were identified as split products chromatographically. The activity of the endolipases was lower. In four of the most active endolipases cholesterol was identified as split product of milk fat in addition to those mentioned above.
mesh_terms: D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000096:biosynthesis; D002855:Chromatography, Thin Layer; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis; D005516:Food Microbiology; D005989:Glycerides / Q000096:biosynthesis; D008049:Lipase / Q000378:metabolism; D050356:Lipid Metabolism; D008848:Micromonosporaceae / Q000378:metabolism*; D008892:Milk / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis
chemical_list: D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D014280:Triglycerides; D002784:Cholesterol; D008049:Lipase
vernacular_title: Lipolytische Aktivität aus Milch isolierter Stämme der Art Thermoactinomyces vulgaris, Tsiklinsky 1899.