Characterization of enhancing factor (EF) prepared from spleen of mice immunized with tetanus toxoid.
In cultures of spleen cells from tetanus toxoid-primed mice a soluble, nonspecific material appeared, which enhanced antibody response in vivo. The active material was purified by gel filtration on Sephadex-G-150 and by affinity chromatography on Con-A lectin. According to the immunological and physiochemical investigation the active material does not contain carbohydrate and its molecular weight is in the 100 000 dalton range.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D007114:Immunization; D007122:Immunoelectrophoresis; D051379:Mice; D008970:Molecular Weight; D013154:Spleen / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*
D000906:Antibodies; D013745:Tetanus Toxoid