title: Demonstration of antibodies to soluble nuclear antigens by complement fixation test. An application of microtiter technique.
abstract: The complement fixation test utilizing the microtiter system was applied to study sera from patients with a variety of connective tissue diseases for the presence of antibodies to soluble nuclear antigens. A good correlation was found between microtiter complement fixation and hemagglutination for anti-RNP and anti-Sm antibodies. Anti-Ha antibodies were detected by complement fixation but not by hemagglutination. Different enzyme digestions of the soluble nuclear antigen prior to assay and use of a quantitative microcomplement fixation test were found to improve the analysis of sera with multiple antibodies.
mesh_terms: D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D002417:Cattle; D002467:Cell Nucleus / Q000276:immunology*; D003095:Collagen Diseases / Q000276:immunology; D003168:Complement Fixation Tests; D006386:Hemagglutination Tests; D006801:Humans; D012261:Ribonucleoproteins / Q000276:immunology
chemical_list: D000906:Antibodies; D012261:Ribonucleoproteins