A radioimmunoassay of primary bile acid conjugates in human serum.
The fasting levels of chenodeoxycholic and cholic acid conjugates in various groups of patients [25 healthy subjects, 20 patients with chronic active liver disease (CALD), 21 patients with primary biliary cirrhosis (PBC) and 5 patients with cholesterol gallstones before and after chenodeoxycholic acid therapy] were determined by using a sensitive, accurate and precise radioimmunoassay. The results obtained with this method were compared with those of a gas-chromatographic method with methylation and acetylation of the bile acids using a 2 m x 0.4 cm glass column packed with 3% of AN 600 on Anakrom Q 110-120 mesh. The results obtained with both methods are in agreement; therefore the method used can be applied for quantitative analysis. In diagnosis, the measurement of serum bile acid levels, fasting or after meals, proved to be a very sensitive method, although further correlations are necessary with standard liver function tests.
D000818:Animals; D000918:Antibody Specificity; D002635:Chenodeoxycholic Acid / Q000097:blood*; D002769:Cholelithiasis / Q000097:blood; D002793:Cholic Acids / Q000097:blood*; D002849:Chromatography, Gas; D006801:Humans; D007106:Immune Sera; D008105:Liver Cirrhosis, Biliary / Q000097:blood; D008107:Liver Diseases / Q000097:blood; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods
D002793:Cholic Acids; D007106:Immune Sera; D002635:Chenodeoxycholic Acid