Radioimmunoassay of minoxidil in human serum.
A simple, sensitive, and specific radioimmunoassay for determining minoxidil was developed. Antiserums to two minoxidil haptens were compared for cross-reactivity and assay levels on human serums. One antiserum had little cross-reactivity with minoxidil metabolites. The radioimmunoassay is specific for determining minoxidil directly in serum without extraction. Human serum minoxidil levels were determined from a single oral dose.
D000818:Animals; D000918:Antibody Specificity; D006801:Humans; D008914:Minoxidil / Q000032:analysis / Q000097:blood* / Q000276:immunology; D011743:Pyrimidines / Q000097:blood*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012685:Sepharose; D012710:Serum Albumin, Bovine; D013997:Time Factors
D011743:Pyrimidines; D012710:Serum Albumin, Bovine; D008914:Minoxidil; D012685:Sepharose