[Parent structures of influenza virus in infected cells].
The fate of influenza virus (A/FPV/Weilbridge) parental structures was studied in permissive (chick fibroblasts) and nonpermissive (Ehrlich ascitic carcinoma cells) cell systems. The cells were infected with the virus labeled with 3H-precursors of RNA of the pulse label and by polymerase reaction in vitro. In the cytoplasm, the functionally active parental structures were found in the area of nucleocapsids (40-50 S). Upon recentrifugation in cesium chloride gradient, some of these structures showed nucleocapsid density of 1.34 g/cm3, and some a higher density (1.40-1.41 g/cm3). In the nucleoplasm, the functionally active structures 2 hours after infection sedimented in the zones of 20-35 S and 3 hours postinfection in the zone of 70-90 S. Both in the cytoplasm and nucleoplasm the parental structures were unstable, undergoing rapid deproteinization. Similar parental structures were found in permissive and nonpermissive cell systems.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002466:Cell Nucleolus / Q000382:microbiology; D002467:Cell Nucleus / Q000382:microbiology; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000382:microbiology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005347:Fibroblasts; D009980:Influenza A virus / Q000302:isolation & purification; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases
Roditel'skie struktury virusa grippa v zarazhennykh kletkakh.