title: Bone cells: a serum-free medium supports proliferation in primary culture.
abstract: Bone-cells isolated from embryonic rat calvaria increase in number two-to threefold when cultured at high, but not at low, population densities in a serum-free medium that contains albumin. Cultured cells respond to parathyroid hormone and exhibit a marked rise in alkaline phosphatase activity during proliferation, which suggests the progressive differentiation or preferential growth of osteoblast-like cells.
mesh_terms: D000818:Animals; D001846:Bone Development; D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D051381:Rats
chemical_list: D003470:Culture Media