The relation between purine metabolism and flavinogenesis in Eremothecium ashbyii. The identification of S-adenosylmethionine and S-adenosylhomocysteine accumulated in non-growing cells of E. ashbyii.
When adenine was added to the non-growing cell medium of Eremothecium ashbyii, riboflavin production of the mold was increasingly inhibited with increasing concentration of adenine. Under these conditions, a cationic compound was accumulated in the mycelia. The compound was isolated from the mycelia and highly purified. The purified compound was proved to be S-adenosylhomocysteine through IR analysis. In the control experiment, or in the addition of other purines which stimulated riboflavin production of the mold, another cationic compound was accumulated in the non-growing cells. The compound was largely accumulated in the presence of both adenine and methionine, isolated from the mycelia and purified. The purified compound was concluded to be S-adenosylmethionine through IR and NMR analyses. The significance of these compounds on the riboflavin biosynthetic pathway was argued under "Discussion".
D000225:Adenine / Q000494:pharmacology; D001203:Ascomycota / Q000378:metabolism*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D006710:Homocysteine / Q000031:analogs & derivatives*; D012256:Riboflavin / Q000096:biosynthesis*; D012435:S-Adenosylhomocysteine / Q000032:analysis / Q000096:biosynthesis*; D012436:S-Adenosylmethionine / Q000032:analysis / Q000096:biosynthesis*; D004718:Saccharomycetales / Q000187:drug effects / Q000378:metabolism*
D003470:Culture Media; D006710:Homocysteine; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; D000225:Adenine; D012256:Riboflavin