Insulin, S-sulphonate A and S-sulphonate B-chain receptor binding to human cultured lymphocytes.
The ability of insulin and the S-sulphonate A and B-chain derivatives to bind to a receptor on cultured human lymphocytes was evaluated. A receptor site for the S-sulphonate A-chain was identified and was strongly influenced by the intact insulin molecule. S-sulphonate A-chain weakly interfered with insulin binding. S-sulphonate B-chain showed no evidence of significant binding and did not interfere with insulin or S-sulphonate A chain binding. 14CO2 production from 14C-1-glucose was stimulated by insulin in cultured lymphocytes and this effect was blunted by S-sulphonate A-chain. The sulphhydryl blocking agent used in the production of insulin A-chain appears to be of critical importance.
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D011384:Proinsulin / Q000378:metabolism; D011972:Receptor, Insulin / Q000378:metabolism*; D013451:Sulfonic Acids / Q000378:metabolism*; D013552:Swine
D007328:Insulin; D007773:Lactates; D010446:Peptide Fragments; D013451:Sulfonic Acids; D002245:Carbon Dioxide; D011384:Proinsulin; D011972:Receptor, Insulin; D005947:Glucose