[Effect of additives on the amination of 2-oxoglutaric acid by gamma-radiation].
The effect of added substances was studied on the yield of glutamic acid produced by gamma-ray irradiation of 2-oxoglutaric acid and ammonia in aqueous solution. The contents of amino acids in the irradiated solutions were determined with amino acids analyzer. Sodium nitrate, allyl alcohol or sodium formate was used as an added substance. The yield of glutamic acid significantly decreased by the addition of nitrate, and it was little affected by the addition of allyl alcohol. In the presence of formate the yield increased from G = 0.4 (2-oxoglutaric acid 0.05M and ammonium hydroxide 2M) to G = 1.1. As a result, it was found that hydrated electron contributes on the formation of glutamic acid, but hydroxyl radical does not. The yield showed a maximum at ca. 0.1 M ammonium hydroxide concentration. These facts indicate that NH2 radical does not contribute to the formation of glutamic acid. As a reaction mechanism, it can be explained that 2-oxoglutaric acid which had been reduced by hydrated electron reacts with ammonia.
D000438:Alcohols; D000498:Allyl Compounds; D000586:Amination; D003037:Cobalt Radioisotopes; D005561:Formates; D005720:Gamma Rays; D005971:Glutamates / Q000138:chemical synthesis*; D007656:Ketoglutaric Acids / Q000528:radiation effects*; D009566:Nitrates; D000644:Quaternary Ammonium Compounds / Q000528:radiation effects*; D012964:Sodium
D000438:Alcohols; D000498:Allyl Compounds; D003037:Cobalt Radioisotopes; D005561:Formates; D005971:Glutamates; D007656:Ketoglutaric Acids; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D012964:Sodium