Stimulation of lymphocytes by concanavalin A. Temperature-dependent effect of fatty acid replacements.
The membrane fatty acyl composition of lymphocytes was altered by growth in lipid-depleted serum containing fatty acid supplements, as well as avidin to block endogenous synthesis of fatty acids. Under these growth conditions over 50% of the total fatty acid in membrane phospholipid were derived from the added fatty acid. Enrichment of lymphocyte membranes with oleate (cis C18:1) or elaidate (trans C18:1) shifted the optimum temperature for mitogenic stimulation by concanavalin A as measured by [3H]thymidine incorporation. These results suggest that the fluidity of the membrane lipid phase plays a role in the process of lymphocyte stimulation by lectins.
D000818:Animals; D001360:Avidin / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D005227:Fatty Acids / Q000378:metabolism*; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D009829:Oleic Acids / Q000378:metabolism; D013696:Temperature
D005227:Fatty Acids; D008563:Membrane Lipids; D009829:Oleic Acids; D003208:Concanavalin A; D001360:Avidin; D004247:DNA