[Electrophoretic patterns of differently prepared fibrinogen subunits in sodium dodecyl sulfate containing polyacrylamide gels].
The electrophoretic behaviour of mercapto, carboxamidomethyl, carboxymethyl and thiosulfonic acid derivatives of rabbit fibrinogen subunits was investigated electrophoretically in sodium dodecyl sulfate containing polyacrylamide gels. Comparing carboxymethyl, carboxamidomethyl and thiosulfonic acid derivatives with the corresponding mercapto subunits divergent electrophoretic patterns were observed. Especially, the position of the Bbeta-chain was strongly dependent on the method of preparation. Similar results were obtained from investigating electrophoretic mobilities of albumin with differently substituted SH-groups after reduction with mercaptoethanol.
D000418:Albumins / Q000032:analysis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000032:analysis*; D006801:Humans; D008623:Mercaptoethanol; D008722:Methods; D010446:Peptide Fragments / Q000032:analysis; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate
D000418:Albumins; D010446:Peptide Fragments; D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol; D005340:Fibrinogen
Elektrophoresemuster unterschiedlich präparierter Fibrinogen-untereinheiten in dodezylsulfathaltigen Polyakrylamidgelen.