[The immunological characterization and isoelectric focusing of water-soluble proteins in the lens related to aging (author's transl)].
Immunelectrophoresis, antigen/antibody crossed electrophoresis and isoelectric focusing are applied to characterize lens crystallins. Using isoelectric focusing alpha-, beta- and gamma-crystallins are determined in bovine lens nucleus and equator. The data obtained are compared with those of rat and human lenses. In these three species a disappearance of specific beta- and gamma-crystallin components is observed depending on age. Further, the presence of crystallins is demonstrated in cornea and vitreous body.
D000375:Aging; D000818:Animals; D002417:Cattle; D002645:Chickens; D003315:Cornea / Q000032:analysis; D003459:Crystallins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D005865:Gestational Age; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007525:Isoelectric Focusing; D007908:Lens, Crystalline / Q000196:embryology / Q000648:ultrastructure*; D008970:Molecular Weight; D051381:Rats; D012995:Solubility; D014822:Vitreous Body / Q000032:analysis
D003459:Crystallins
Charakterisierung der wasserlöslichen Proteine der Linse mittels Immunologie und Isoelektrofokussierung und ihre Beziehungen zum Alterungsproze