The reactivity of human platelets in the 125I-labelled staphylococcal protein A test.
Stored platelets have been employed in the testing of various antiplatelet antibodies and platelet unrelated immune complexes. The antisera included sera containing ABO-,HLA- or platelet specific (ZWa) antibodies and serum from an adult patient with idiopathic thrombocytopenic purpura (ITP). Use was made of 125I-labelled staphylococcal protein A (IPA) for the detection of platelet bound IgG. The reactivity of cells with the ITP serum and sera from healthy blood donors increased after storage for a few days, and declined after a highly reactive period extending over some weeks. In some cases predilution of the serum increased the probability of discovering weak antibodies. The normal components of the serum modified the platelet-antibody reaction, but all the various kinds of platelet reactive activities could be detected by this single test.
D000017:ABO Blood-Group System; D000936:Antigen-Antibody Complex; D001792:Blood Platelets / Q000276:immunology*; D002470:Cell Survival; D002852:Chromatography, Ion Exchange; D005557:Formaldehyde / Q000494:pharmacology; D006680:HLA Antigens; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G / Q000032:analysis; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D009439:Neuraminidase / Q000494:pharmacology; D011696:Purpura, Thrombocytopenic / Q000276:immunology; D011863:Radioimmunoassay; D013205:Staphylococcal Protein A / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology
D000017:ABO Blood-Group System; D000936:Antigen-Antibody Complex; D006680:HLA Antigens; D007106:Immune Sera; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D013205:Staphylococcal Protein A; D005557:Formaldehyde; D009439:Neuraminidase; D014357:Trypsin