Effects of a tumor-promoting agent on chondrogenesis.
Chondroblasts exposed to the phorbol ester, PMA, rapidly lose their polygonal morphology, and rapidly cease to synthesize or accumulate the chondroblast-specific Type IV sulfated proteoglycan. This striking effect is reversible if the cells are kept in PMA for up to 72 hours. A longer exposure induces irreversible effects and results in a population of cells, the vast majority of which lack the phenotypic properties of terminal chondroblasts.
D000818:Animals; D002273:Carcinogens; D002356:Cartilage / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D010704:Phorbols / Q000494:pharmacology*; D011509:Proteoglycans / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002273:Carcinogens; D010704:Phorbols; D011509:Proteoglycans; D013755:Tetradecanoylphorbol Acetate