Cobalamins and cobalamin-dependent enzymes in Candida utilis.
Candida utilis has been shown to contain 4.7 pmol of cobalamin per g of wet cell paste. Purification of the cobalamin showed it to be a mixture of methylcobalamin and adenosylcobalamin. Two cobalamin-dependent enzyme systems have been found in the yeast: methylcobalamin-dependent methionine biosynthesis and leucine 2,3-aminomutase. The cobalamin extracted from the yeast is as effective as authentic adenosylcobalamin in stimulating leucine 2,3-aminomutase.
D000591:Amino Acid Isomerases / Q000032:analysis* / Q000378:metabolism; D002175:Candida / Q000032:analysis* / Q000378:metabolism; D002474:Cell-Free System; D019751:Intramolecular Transferases; D007930:Leucine / Q000096:biosynthesis; D008715:Methionine / Q000096:biosynthesis; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology
D008715:Methionine; D000591:Amino Acid Isomerases; D019751:Intramolecular Transferases; C021507:leucine 2,3-aminomutase; D007930:Leucine; D014805:Vitamin B 12