Detection of antibody to rubella virus by enzyme-linked immunosorbent assay.
Titers of antibody to rubella virus in 68 human sera were compared by hemagglutination inhibition and enzyme-linked immunosorbent assay (ELISA). In general, the titers measured by ELISA were higher than those found by hemagglutination inhibition. Although the titers differed, the two methods showed parallel trends. Use of purified rubella virus and addition of 1% bovine serum albumin to the test wash solution aided in reducing false-positive titers in ELISA.
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D011817:Rabbits; D012412:Rubella virus / Q000276:immunology*
D000914:Antibodies, Viral