Different pools of free myoinositol in chick-embryo cells as indicated by infection with Newcastle-disease virus.
Infection of chicken fibroblasts with Newcastle-disease virus indicates that cellular inositol is compartmented in at least two pools. Only the smaller pool is directly connected with the biosynthesis of phosphatidylinositol. Entrance of exogenous inositol into this pool is inhibited by phlorizin but not by the virus. Three hours after infection Newcastle-disease virus blocks the entrance of inositol from the small pool into one (or more) subsequent larger pool(s). About five hours after infection the virus enhances the catabolism of phosphatidylinositol in chicken cells and about seven hours after infection the permeability of the plasma membrane increases.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D007294:Inositol / Q000378:metabolism*; D009522:Newcastle disease virus / Q000378:metabolism*; D010695:Phlorhizin / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D014779:Virus Replication
D010716:Phosphatidylinositols; D007294:Inositol; D010695:Phlorhizin