Evidence for a DNA ligase change related to early cleavage in axolotl egg.
A definite change in the forms of DNA ligase appears when the axolotl egg enters cleavage. Sucrose gradient and phosphocellulose chromatography show that the a 6S form of DNA ligase exists before division, i.e. in unfertilised and fertilised egg, and a 8.2S form is present at the first division. N-ethylmaleimide sensitivity and heat stability are different for the two forms. The possible significance of this early change is discussed.
D000557:Ambystoma / Q000502:physiology*; D000818:Animals; D002455:Cell Division; D011088:DNA Ligases / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D006358:Hot Temperature; D010063:Ovum / Q000201:enzymology*; D011118:Polynucleotide Ligases / Q000378:metabolism*; D015053:Zygote / Q000201:enzymology*
D011088:DNA Ligases; D011118:Polynucleotide Ligases; D005033:Ethylmaleimide