title: Growth and reproductive development in freemartins hormonally treated from 1 to 79 weeks of age.
abstract: Postnatal growth, steroid hormone profiles and response to steroid hormone treatment were studied in 18 freemartins and one normal female born co-twin with a bull. They were either treated postnatally with testosterone or estrone at one week of age, or left untreated until 50 weeks of age when they received silastic implants calculated to release either 12.9 microgram of estrone or 2.6 microgram of estradiol per day per kg of body weight. Later a dihydrotestosterone-treated group was added. Reproductive development was studied by palpation per rectum and by examination when animals were slaughtered at 79 weeks of age. Treated animals grew slightly faster than untreated animals. Testosterone in untreated freemartins averaged 76 and 87 pg/ml of blood plasma during weeks 1 to 48 and 52 to 56. Corresponding values for those animals with small testosterone implants (weeks 1 to 48) and with larger implants (weeks 52 to 56) were 130 and 272 pg/ml. Estrone and estradiol values appeared to fluctuate between 10 and 50 pg/ml but values are uncertain because they were below the sensitivity of the assay then available. Thus, circulating steroid hormone concentrations were similar to those reported for castrates. Testosterone stimulated clitoral development prenatally and postnatally. None of the treatments influenced vaginal depth, which averaged 4.0, 9.0 and 10.9 cm at 1, 24 and 52 weeks of age. Vaginal depth at birth was not a reliable indicator of freemartinism. Androgen may have inhibited udder development, whereas estrogen appeared to be stimulatory. The reproductive organs of the freemartin were characterized by differences in underdevelopment and the general presence of seminal vesicles. The latter structures, plus clitoral development at birth in 3 animals and postnatal response of the clitoris to testosterone is interpreted to indicate that the presence of androgens is one factor in abnormal development. Otherwise, gross morphology of the reproductive tract was not related to hormone treatment, postnatal gonadal histology, endocrinology or lymphocyte chromosomal karyotypes.
mesh_terms: D000818:Animals; D002417:Cattle / Q000254:growth & development*; D002987:Clitoris / Q000254:growth & development; D004958:Estradiol / Q000097:blood / Q000494:pharmacology; D004970:Estrone / Q000097:blood / Q000494:pharmacology; D005260:Female; D005611:Freemartinism / Q000503:physiopathology*; D005835:Genitalia / Q000254:growth & development*; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D008297:Male; D008321:Mammary Glands, Animal / Q000254:growth & development; D010053:Ovary / Q000254:growth & development; D012669:Seminal Vesicles / Q000254:growth & development; D013739:Testosterone / Q000097:blood / Q000494:pharmacology; D014621:Vagina / Q000254:growth & development
chemical_list: D012739:Gonadal Steroid Hormones; D004970:Estrone; D013739:Testosterone; D004958:Estradiol