The influence of rRNA and tRNA on the translation of avian globin mRNA in cell-free systems of protein synthesis.
Cell-free synthesis of globin chains in the presence of globin mRNA in an Ehrlich ascites-cell-free system is further stimulated by addition of 18- and 28-S rRNA but not of 4-S tRNA and 5-S rRNA. This stimulation can not be observed in the wheat germ cell-free system. When 125I-labelled globin mRNA was incubated in the two systems we have found after 60 min a 75% decrease of trichloroacetic acid precipitable polynucleotides in the ascites but only a 20% decrease in the wheat germ system. The RNAase action on mRNA can be reduced by the addition of 18- and 28-S rRNA but not by 5-S rRNA and 4-S tRNA. We suggest that the stimulating effect of the two rRNA species in the ascites cell-free system is due to a higher activity of a specific RNAase in this system and a competitive protection of mRNA from RNAase action.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002474:Cell-Free System; D005914:Globins / Q000235:genetics*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012335:RNA, Ribosomal / Q000494:pharmacology*; D012343:RNA, Transfer / Q000494:pharmacology*; D012260:Ribonucleases / Q000037:antagonists & inhibitors; D014908:Triticum
D012335:RNA, Ribosomal; D005914:Globins; D012343:RNA, Transfer; D012260:Ribonucleases