Lipid composition of cells and its variation during degradation of a culture of Streptococcus diacetilactis.
The process of dying of a culture of Str. diacetilactis under conditions of prolonged culturing is accompanied by substantial changes in the composition of the cell lipids. In the lipids of young cells the basic components are mono- and diglucosyldiglycerides, as well as phospholipids--two glucophospholipids, cardiolipin, and phosphatidylglycerin; triglycerides and sterols are present in negligible amounts. As the Str. diacetilactis culture develops and undergoes degradation, an increase is observed in the total amount of extractable lipids. In this case the fraction of phospholipids increases (chiefly on account of cardiolipin), while the fraction of neutral glucolipids decreases. It was shown that in cell homogenates there is a negligible peroxidation of lipids, which is intensified as the culture dies off. There is a parallel acceleration of the uptake of O2 by cell suspensions from cultures of different ages. The detected changes in the lipids are correlated with the morphological and physiological indices of the state of the culture.
D002241:Carbohydrates / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D008054:Lipid Peroxides / Q000096:biosynthesis; D008055:Lipids / Q000032:analysis*; D008563:Membrane Lipids / Q000032:analysis; D010101:Oxygen Consumption; D010743:Phospholipids / Q000032:analysis; D013261:Sterols / Q000032:analysis; D013291:Streptococcus / Q000032:analysis* / Q000378:metabolism
D002241:Carbohydrates; D005227:Fatty Acids; D008054:Lipid Peroxides; D008055:Lipids; D008563:Membrane Lipids; D010743:Phospholipids; D013261:Sterols; D005990:Glycerol