Use of automated chromatography on the amino acid analyzer with lithium citrate buffers to separate nucleic acid bases, nucleosides, nucleotides and their precursors.
A simple and sensitive method has been developed to separate nucleic acid bases, nucleosides, nucleotides and their precursors by automated chromatography using the amino acid analyzer with lithium citrate buffers. The method is sensitive to a concentration of 5 nmol, linear in the range of 5--100 nmol, and resolves almost all the bases, nucleosides, nucleotides and their precursors of physiologic importance.
D001322:Autoanalysis / Q000379:methods; D002021:Buffers; D002852:Chromatography, Ion Exchange / Q000379:methods; D002951:Citrates; D008832:Microchemistry; D009705:Nucleosides / Q000302:isolation & purification*; D009711:Nucleotides / Q000302:isolation & purification*
D002021:Buffers; D002951:Citrates; D009705:Nucleosides; D009711:Nucleotides