[Acetylcholinesterase activity, phospholipids, cholesterol and sulphydryl group content in sarcolemma fragments].
The phospholipid, cholesterol, SH-group content and acethylcholinesterase activity in ultrasonic sarcolenima fragments precipitating at 105000 g were much greater than in fragments precipitating at 3000 g. On the basis of the results obtained it is suggested that the 3000 g sediment consists largely of network of collagen fibrils, while the 105 000 g sediment is mainly composed by the plasma membrane fragments. It is shown that the amount of SH-groups in intact sarcolemma available for 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNG) in the presence of sodium dodecylsulphate was about twice as much as that in the absence of sodium dodecylsulphate. As to the sonicated fractions of sarcolemma (sediment and supernatant at 105 000 g) and high ionic strength extract of sarcolemma the amount of SH-groups available for DTNB in the presence and in the absence of sodium dodecylsulphate was similar. A decrease was observed in acetylcholinesterase stability after sonication (sediment and supernatant at 105 000 g). The normal Michaelis kinetics was found for the acetylcholinesterase of sarcolemma fractions solubilized by sonication.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002458:Cell Fractionation; D002784:Cholesterol / Q000032:analysis*; D004228:Dithionitrobenzoic Acid; D007700:Kinetics; D009124:Muscle Proteins / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D011817:Rabbits; D012508:Sarcolemma / Q000032:analysis* / Q000201:enzymology; D013438:Sulfhydryl Compounds / Q000032:analysis
D009124:Muscle Proteins; D010743:Phospholipids; D013438:Sulfhydryl Compounds; D002784:Cholesterol; D004228:Dithionitrobenzoic Acid; D000110:Acetylcholinesterase
Atsetilkholinesteraznaia aktivnost' soderzhanie fosfolipidov kholesterina i sul'fgidril'nykh grupp vo fragmentakh sarkolemmy.