Solubilization of bovine corpus-luteum adenylate cyclase in lubrol-PX, triton X-100 or digitonin and the stabilizing effect of sodium fluoride present in the solubilization medium.
1. Adenylate cyclase activity of the washed 600g sediment of bovine corpus-luteum homogenate was solubilized by Lubrol-PX, Triton X-100 and digitonin. Digitonin was the least destructive of NaF-stimulated activity. 2. NaF, present in the solubilization medium together with MgSO4, increased the percentage yields of soluble activity from untreated 600g sediment and 600g sediment which had been preincubated with p[NH]ppG (guanosine 5'-[betagamma-imido]triphosphate). The stabilizing influence of NaF was most marked with digitonin. However, the highest specific activities of soluble enzyme were obtained with Lubrol-PX as solubilizing agent, since digitonin solubilized more membrane protein than does Lubrol-PX, and less of the activity of the digitonin-dispersed 600g sediment was recovered in the 105000g supernatant. 3. p[NH]ppG also has a stabilizing effect when present during the solubilization, but less so than NaF. 4. Both NaF and MgSO4 alone have a stabilizing effect during solubilization. The greatest amounts of soluble activity were obtained with both agents present in the solubilization medium, there being a synergistic effect.
D000262:Adenylyl Cyclases; D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000201:enzymology*; D003902:Detergents; D004072:Digitonin; D005260:Female; D005459:Fluorides; D006165:Guanylyl Imidodiphosphate; D008274:Magnesium; D011092:Polyethylene Glycols; D012969:Sodium Fluoride; D012996:Solutions
D003902:Detergents; D012996:Solutions; D006165:Guanylyl Imidodiphosphate; D011092:Polyethylene Glycols; D012969:Sodium Fluoride; D000262:Adenylyl Cyclases; D008274:Magnesium; D004072:Digitonin; D005459:Fluorides