Effect of lipid antioxidants on rat erythrocyte hemolysis.
Lipid antioxidants, DPPD, DAH, BHT, SQ, retinol and alpha-tocopherol were studied for their effects on normal rat erythrocytes. Retinol, BHT and SQ were found to induce hemolysis while DPPD, DAH and alpha-tocopherol were non-hemolytic. Further more the three antioxidants BHT, SQ and retinol showed a synergistic effect on the lysis. Retinol, BHT or SQ alone, and BHT or SQ along with retinol when administered to rats produced a marked modification of the erythrocyte membrane integrity, simultaneously lowering the levels of membrane bound enzymes--acetyl choline esterase and ATPase. It is concluded that the lipid antioxidants may therefore be classified on the basis of their lytic action in vitro.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D006873:Hydroquinones / Q000494:pharmacology; D008297:Male; D009996:Osmotic Fragility; D010655:Phenylenediamines / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D051381:Rats; D014801:Vitamin A / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
C026297:2,5-ditertiary amyl hydroquinone; D000975:Antioxidants; D006873:Hydroquinones; D010655:Phenylenediamines; D011804:Quinolines; D014801:Vitamin A; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; C004845:N,N'-diphenyl-4-phenylenediamine