[Fractionation of DNA containing single-stranded regions on a column with benzoylated DEAE-cellulose].
A possible use of a previously described method of fractionation of highly polymeric DNAs on a column with benzoylated DEAE-cellulose is discussed. The method can be used for separation of renaturated and hybrid DNA molecules as well as for detection of single-stranded structures within the DNA. Using the method in question it was shown that the DNA of hepatoma 27 contains about 6% of single-stranded DNA structures.
D000818:Animals; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D005399:Fishes; D008114:Liver Neoplasms, Experimental / Q000032:analysis; D051381:Rats
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D004247:DNA
Fraktsionirovanie DNK, soderzhashchikh odnonitevye uchastki, na kolonke s benzoilirovannoĭ DEAE-tselliulozoĭ.