Ribulose-peptide in human semen: I. Isolation.
A substance apparently related to a compound consisting of phospho-peptide and ribulose-peptide in the yolk of chick embryos was isolated from acid soluble fractions of human semen by means of Dowex 1 (OH) form column chromatography. The treated precipitate of human semen was examined with an analytical ultracentrifuge by means of synthetic boundary cells and found to be a single component. The sedimentation coefficient was 0.80. The sugar component in the precipitate was identified as ribulose by paper chromatography, color reaction, absorption spectrum of the reaction product in the orcinol, and enzymatic analysis. Amino acids detected in the acid hydrolysate of the precipitate were lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, and leucine. From the above, this substance was determined to be a ribulose-peptide.
D002854:Chromatography, Paper; D006801:Humans; D008297:Male; D010429:Pentoses / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*; D012661:Semen / Q000032:analysis*
D010429:Pentoses; D010455:Peptides