Thymidine photoproducts of non-cyclobutane type as a crucial event in the conformation of UV-irradiated DNA.
The UV-light induced conformational effects in deoxyoligonucleotides and polynucleotides have been analyzed by CD measurements and isolation of the photoproducts. The results demonstrate that the essential photoproduct formed on irradiation of thymidylyl-thymidine at 254 nm is of non-cyclobutane type and may be correlated to the primary photoproduct formed in DNAs at low doses Formation of thymine dimers of cyclobutane-type structure appears to be a secondary product generated by treatment with formic acid.
D002854:Chromatography, Paper; D002942:Circular Dichroism; D003503:Cyclobutanes; D005561:Formates / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000528:radiation effects*; D009841:Oligonucleotides / Q000528:radiation effects*; D010777:Photochemistry; D011067:Poly dA-dT / Q000528:radiation effects*; D011089:Polydeoxyribonucleotides / Q000528:radiation effects*; D011740:Pyrimidine Dimers / Q000096:biosynthesis; D013936:Thymidine / Q000528:radiation effects*; D014466:Ultraviolet Rays
D003503:Cyclobutanes; D005561:Formates; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011089:Polydeoxyribonucleotides; D011740:Pyrimidine Dimers; D011067:Poly dA-dT; D013936:Thymidine