Production of labile Newcastle disease virus progeny after infection of chicken embryo cells in the presence of caffeine.
A study was undertaken to examine the effects of caffeine on Newcastle disease virus (NDV) infection of chicken embryo cells. Addition of 10 mM caffeine to the growth medium produced 95% reduction in progeny synthesis, 63% reduction in RNA synthesis, 45% reduction in protein synthesis, and 25% reduction in hemadsorption ability in NDV-infected cultures when compared with untreated, infected cultures. Purified NDV obtained from caffeine-treated, infected cultures was more sensitive to ultraviolet irradiation and to damage by freezing and thawing than was observed in untreated virus cultures. The SDS-polyacrylamide gel electrophoresis revealed that purified virions contained the same complement of polypeptides, but there was a significant variation in the quantities of several of the NDV polypeptides.
D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D009522:Newcastle disease virus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D010455:Peptides / Q000032:analysis; D012367:RNA, Viral / Q000096:biosynthesis; D013696:Temperature; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis
D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins; D002110:Caffeine