Cytogenetic effects of busulfan in vivo on bone marrow cells and oocytes of adult mice and liver cells of transplacentally exposed embryos.
Adult female mice were twice treated orally with 15, 30, or 60 mg busulfan per kg of body weight. In bone marrow, a dose-dependent linear increase in the frequency of chromosome aberrations was observed. Similar to the effects of other alkylating agents, busulfan induced all types of aberrations, including exchanges. In metaphase II oocytes, only a few chromosome aberrations were observed. An intervening round of DNA-replication seems to be necessary to translate busulfan-induced lesions in oocytes into chromosome aberrations. In embryonic liver cells of transplacentally exposed mice, chromosome aberrations were induced.
D000818:Animals; D001853:Bone Marrow / Q000648:ultrastructure; D002066:Busulfan / Q000494:pharmacology*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D008099:Liver / Q000196:embryology; D051379:Mice; D009153:Mutagens; D009865:Oocytes / Q000648:ultrastructure
D009153:Mutagens; D002066:Busulfan