The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography.
Phosphatidylserine has been prepared from phosphatidylcholine by a one-step transphosphatidylation catalyzed by phospholipase D in the presence of L-serine. The resulting mixture of phosphatidylserine and phosphatidic acid is easily and rapidly separated by CM-cellulose column chromatography using step=wise elution with solvents containing increasing percentages of methanol in chloroform. The over-all yield of the procedure is 40-50% depending on the scale of the preparation. CM-Cellulose column chromatography proved to be extremely useful in separating phospholipid mixtures obtained by phosphatidyltransferase reactions of phospholipase D and is also suitable for fractionation of other lipid extracts.
D002852:Chromatography, Ion Exchange; D004530:Egg Yolk; D005260:Female; D007700:Kinetics; D010713:Phosphatidylcholines; D010718:Phosphatidylserines / Q000096:biosynthesis* / Q000302:isolation & purification; D010740:Phospholipases; D010743:Phospholipids; D013329:Structure-Activity Relationship
D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D010743:Phospholipids; D010740:Phospholipases