Hormonal effects on canine prostatic explants in organ culture.
Explants of canine prostate were cultured in a defined medium for periods of up to 5 days with and without added steroids. Testosterone and 5 alpha-dihydrotestosterone failed to maintain their histological integrity and induced a greatly increased formation of stromal elements. Epitestosterone and 5 alpha-dihydroepitestosterone were partially successful in maintaining epithelial height although secretory activity was not preserved. The only steroid that sustained epithelial height and secretory activity whilst keeping stromal growth at a minimum was 5 alpha-androstane-3 alpha, 17 alpha-diol. The three other epimeric androstanediols were ineffective.
D015113:Androstane-3,17-diol / Q000494:pharmacology; D000731:Androstanes / Q000494:pharmacology*; D000818:Animals; D013196:Dihydrotestosterone / Q000494:pharmacology; D004285:Dogs; D004845:Epitestosterone / Q000494:pharmacology; D008297:Male; D009924:Organ Culture Techniques; D011467:Prostate / Q000166:cytology / Q000187:drug effects*; D013739:Testosterone / Q000494:pharmacology
D000731:Androstanes; D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol; D013739:Testosterone; D004845:Epitestosterone