Inhibitor-neutralisation assay and electro-immuno assay of human factor IX (Christmas factor).
A rabbit antibody specifically precipitating human factor IX has been used in the assay of factor IX antigen. The results obtained with two different methods (inhibitor-neutralisation assay and electro-immunoassay) have been compared in a group of healthy individuals and in a group of hemophilia B patients and carriers. In general, identical results are obtained with both methods, except in some hemophilia B+ carriers and patients, where the electroimmuno assay gives 1.5-2.0 times higher levels. Results obtained by electroimmuno assay are more accurate and reproducible than those obtained by inhibitor-neutralisation assay, which is of importance for its potential use in carrier detection.
D000328:Adult; D000818:Animals; D000918:Antibody Specificity; D000941:Antigens; D005069:Evaluation Studies as Topic; D005164:Factor IX / Q000032:analysis* / Q000276:immunology; D005260:Female; D002836:Hemophilia B / Q000097:blood; D006801:Humans; D007106:Immune Sera; D007118:Immunoassay; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D009500:Neutralization Tests; D011817:Rabbits / Q000276:immunology
D000941:Antigens; D007106:Immune Sera; D005164:Factor IX