Biosynthesis of gibberellins in Gibberella fujikuroi. Gibberellin A47.
Two minor radioactive products in cultures of G. fujikuroi strain ACC917 supplemented with labeled gibberellin A4 have been identified as the 2alpha-hydroxy derivative, gibberellin A47, and the hydrate, gibberellin A2. In addition, evidence was obtained by combined gas chromatography--mass spectrometry for the formation of 3-O-acetylgibberellin A1,3-O-acetylgibberellin A3, and gibberellin A20 by the culture. The time course of gibberellin synthesis in defined and complex media, as well as changes in the relative amounts of radioactive gibberellins formed when [4C]gibberellin A4 was added to cultures at different ages, suggest that the composition of the gibberellin mixture produced is determined by the balance between synthesis and decay of key enzymes in the branching pathway.
D005874:Gibberella / Q000378:metabolism*; D005875:Gibberellins / Q000096:biosynthesis* / Q000302:isolation & purification; D005947:Glucose / Q000378:metabolism; D006999:Hypocreales / Q000378:metabolism*; D007700:Kinetics; D013025:Glycine max
D005875:Gibberellins; D005947:Glucose