title: Hormone receptor levels and hormone, RNA and protein metabolism in the genital tract during the oestrous cycle of the ewe.
abstract: The concentrations of soluble oestradiol and progesterone receptor proteins and several metabolic activities were measured in the genital tracts of ewes killed on Days 0 (oestrus), 2, 5, 10 or 14 of the oestrous cycle. In caruncular endometrium and in whole uterus the concentrations of oestradiol receptor (pmol steroid bound/mg tissue DNA) were highest at oestrus and declined steadily thereafter to minimal values at Day 14. The concentrations of progesterone receptor were highest on Days 0-5, then declined to low levels on Day 10-14. There was little metabolism of either [3H]oestradiol or [3H]progesterone in minces of whole uterus and with either steroid the pattern of metabolism did not change at any stage of the cycle. The in-vivo rates of synthesis of protein in caruncular endometrium and in isthmic oviduct were highest at or shortly after oestrus (Days 0-2), then declined to low levels on Days 10-14. RNA:DNA ratios in these two tissues were also greatest at oestrus and subsequently fell to minimal values by Day 14. The results are discussed in relation to ovarian secretion of oestradiol and progesterone during the oestrous cycle of the ewe.
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000033:anatomy & histology; D004247:DNA / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D004971:Estrus; D005260:Female; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D012756:Sheep / Q000502:physiology*; D013997:Time Factors; D014599:Uterus / Q000378:metabolism*
chemical_list: D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011374:Progesterone; D004958:Estradiol; D012313:RNA; D004247:DNA