[Application of nitrocellulose Nitrocell S for preparation of mouse globin mRNA].
Two methods of chromatography were applied and compared to purify individual mRNA for mouse globin. In one case commercial product Nitrocell S was used, in another affinity chromatography on oligo-dT-cellulose was applied. Translation of purified mRNA was performed in the heterological wheat germ system in vitro. Products of translation were identified as alpha- and beta-chains of mouse globin. The data obtained permits us to recommend Nitrocell S as efficient and cheap chromatographic material for purifing poly-A-containing RNA.
D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives*; D002846:Chromatography, Affinity; D005914:Globins / Q000096:biosynthesis*; D051379:Mice; D011061:Poly A; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification*; D014908:Triticum / Q000378:metabolism
D012333:RNA, Messenger; D011061:Poly A; D005914:Globins; D002482:Cellulose
Ispol'zovanie nitrotselliulozy Nitrocell S dlia vydeleniia mRNK globina myshi.