Cytotoxic effects of phallolysin on a human heteroploid cell line.
The effect of Phallolysin on cellular growth, macromolecular biosyntheses and cellular membrane structure was analysed using cells from the EUE line. Concentrations of the toxin that do not affect cellular growth, as determined by plating efficiency, have no effect on RNA or protein synthesis, but stimulate DNA synthesis. The doses of Phallolysin that inhibit cell survival do not affect macromolecular biosyntheses, but greatly increase the percentage of cells stainable with Trypan blue after 1 hour of incubation. At the same dose of the toxin the cells, analysed by electron microscopy, show increased vacuolization indicating an alteration of the membrane apparatus.
D000546:Amanitins / Q000633:toxicity*; D000782:Aneuploidy; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D002869:Chromosome Aberrations / Q000187:drug effects; D003603:Cytotoxins; D004305:Dose-Response Relationship, Drug; D006651:Histocytochemistry; D006801:Humans; D008854:Microscopy, Electron; D008938:Mitosis / Q000187:drug effects; D009696:Nucleic Acids / Q000096:biosynthesis; D014176:Protein Biosynthesis
D000546:Amanitins; D003603:Cytotoxins; D009696:Nucleic Acids; C008824:phallolysine