title: Lysis of C1Q-coated chicken erythrocytes by human lymphoblastoid cell lines.
abstract: Human lymphoblastoid cells lysed chicken erythrocytes (E) that carried cell surface bound human C1q. Antibody to E(A) was not required for the C1q-dependent reaction. The effect of C1q was inhibited by Fab'2 anti-C1q and by the serum C1q inhibitor. The action of the lymphoblastoid cells was inhibited by anti-metabolites and by pretreatment of the cells with trypsin which is known to destroy their C1q receptor. Lymphoblastoid cell lysate was inactive. The time course of the C1q-dependent lysis was comparable to that of the antibody-dependent cellular cytotoxic reaction of human K-cells. Lysis of EA by human peripheral lymphocytes was enhanced up to 50% by human C1q.
mesh_terms: D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000963:Antimetabolites / Q000494:pharmacology; D002460:Cell Line; D002645:Chickens; D003172:Complement C1; D003174:Complement C1 Inactivator Proteins / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D008214:Lymphocytes / Q000276:immunology*
chemical_list: D000963:Antimetabolites; D003172:Complement C1; D003174:Complement C1 Inactivator Proteins; D007140:Immunoglobulin Fab Fragments