Sterilization of hepatitis B surface antigen-contaminated materials.
The sterilizing conditions of HBs Ag-contaminated materials were studied by means of the inactivation of the antigenicity (infectivity being thought to be lost when HBs Ag is inactivated). HBs Ag-coated beads and the serum from an HBs Ag-positive patient were used. After each sterilizing procedure, the antigenicity was studied by radioimmunoassay. HBs Ag-positive serum is easily inactivated by boiling and by steam under pressure; however, HBs Ag-coated beads require increased steam under pressure. For sterilizing HBs Ag-contaminated materials, 30 minutes at 132 degrees C is recommended.
D001769:Blood / Q000276:immunology; D002495:Central Supply, Hospital / Q000379:methods; D004864:Equipment and Supplies; D005027:Ethylene Oxide / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D006514:Hepatitis B Surface Antigens; D011312:Pressure; D011863:Radioimmunoassay; D013227:Steam; D013242:Sterilization / Q000379:methods*; D013997:Time Factors
D006514:Hepatitis B Surface Antigens; D013227:Steam; D005557:Formaldehyde; D005027:Ethylene Oxide