title: Iso-renin secretion from rat brain cortex slices in vitro under the influence of ions and electrical stimulation.
abstract: The aim of this study was to obtain additional data concerning the influence of changes in osmolarity and ionic composition on iso-renin secretion from rat brain cortex slices. The slices were isolated and superfused in vitro according to the technique of Pull and McIlwain (1972). The secretion of iso-renin was strongly stimulated by an increase of Na+ concentration. Iso-renin output almost doubled upon raising NaCl from 120 to 240 mM. Decrease of the NaCl concentration to 60 mM resulted in a reduced iso renin secretion while addition of tetrodotoxin (TTX) (60 micron) did not significantly alter the iso-renin content of brain slices. Changes in osmotic pressure were without significant influence. Electrical stimulation or elevation of extracellular K+ concentrations enhanced iso-renin secretion. Furthermore, Ca2+ ions seem to increase both the content and the release of cerebral iso-renin.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002540:Cerebral Cortex / Q000378:metabolism* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009994:Osmolar Concentration; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D012083:Renin / Q000378:metabolism* / Q000502:physiology; D012964:Sodium / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D012965:Sodium Chloride; D012964:Sodium; D012083:Renin; D011188:Potassium; D002118:Calcium