title: Analysis of chicken ribosomal RNA genes and construction of lambda hybrids containing gene fragments.
abstract: The ribosomal RNA genes (ribosomal DNA) of chicken are present in approximately 200 copies and are cleaved into two fragments of molecular weight 5 X 10(6) and 12 to 14 X 10(6) by restriction endonuclease Eco RI. Recombinant phages have been constructed in vitro by joining the smaller fragment of ribosomal DNA and the outer arms of DNA from the vector lambdagtWES-lambdaC. In one of the recombinants, the coding strand of the cloned fragment is in the proper orientation for transcription with lambda "early" genes; in the other two the orientation is reversed, with the coding strand in the proper position for transcription with lambda "late" genes.
mesh_terms: D000818:Animals; D002645:Chickens; D002874:Chromosome Mapping; D003090:Coliphages; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000032:analysis* / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D005796:Genes; D008970:Molecular Weight; D012335:RNA, Ribosomal; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes