title: Marsupial cells in long-term culture.
abstract: Cell lines have been developed from several species of Australian marsupials and studied during long-term growth. Cell lines developed from macropodid skin or heart tissues all had reproducible finite life-spans. However, cell lines developed from dasyurids showed bariable behavior in culture: lines developed from Antechinus stuartii and Dasyurus viverrinus had finite life-spans, while lines developed from Sminthopsis crassicaudata had indefinite life-spans. S. crassicaudata lines usually became heteroplloid, but one was still diploid after 150 population doublings, while another contained a proportion (10%) of haploid cells. Other lines were developed from the peramelid, Perameles nasuta, and the phanlngerid, Trichosurus vulpecula.
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D004171:Diploidy; D008168:Lung; D008297:Male; D008394:Marsupialia; D009206:Myocardium; D012867:Skin; D013045:Species Specificity; D013737:Testis; D013997:Time Factors
chemical_list: D003470:Culture Media