Studies on pulse-labelled RNA during the mitotic cycle of Physarum polycephalum by subnuclear fractionation.
A method is described for the isolation of pulse-labelled RNA from nuclei and subnuclear fractions of Physarum polycephalum. At all times during interphase the nucleolar RNA consisted mainly of a 34-S rRNA precursor with only small amounts of 26-S and 19-S rRNAs. The nucleoplasmic RNA consisted of predominantly mature 26-S rRNA with small amounts of 19-S rRNA and a 30-S RNA species. The 30-S RNA component displayed different labelling kinetics from the rRNA precursors. A low molecular weight RNA fraction (4-8 S) appeared to accumulate in the nucleus as interphase progressed.
D002467:Cell Nucleus / Q000032:analysis; D008938:Mitosis; D009235:Myxomycetes / Q000378:metabolism*; D010804:Physarum / Q000166:cytology / Q000378:metabolism*; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D013347:Subcellular Fractions / Q000032:analysis
D012313:RNA