[The content and types of sulfhydryl groups in muscle creatine kinase detected by their accessibility to different reagents].
Using three independent methods tte amperometric titration, Boyer's method and the method of Ellman it is shown that rabbit muscle creatine kinase contains 11-12 SH-groups, 3-6 of which are easily oxidized by atmospheric oxygen to form S-S-bonds. It is found that creatine kinase has four types of SH-groups distinguished by their accessibility to different SH-reagents. The first type related to the enzymatic activity is detected by the Ellman method (2 SH-groups), the first and the second ones taken together--by the Boyer method (4 SH-groups), the first, second and third ones--by the method of amperometric titration (6 SH-groups), all the 4 types together--when detecting SH-groups after protein denaturation--by any of the above methods (8-12 SH-groups).
D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D004220:Disulfides; D009132:Muscles / Q000201:enzymology*; D010084:Oxidation-Reduction; D011817:Rabbits; D013438:Sulfhydryl Compounds / Q000032:analysis*; D013439:Sulfhydryl Reagents
D004220:Disulfides; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D003402:Creatine Kinase
Soderzhanie i tipy sul'fgidril'nykh grupp kreatinkinazy, otlichaiushchikhsia po ikh dostupnosti dlia razlichnykh reagentov.