Ion-exchange chromatographic study of amino acid degradation during hydrolysis of avian protein.
The losses of amino acid during acid hydrolysis of oviduct magnum protein from the domestic fowl have been measured using a Technicon TSM-1 amino acid analyser. The results were subjected to statistical analysis and a linear regression was obtained for each amino acid investigated. Methionine, in the presence of oxygen, did not exhibit significant degradation. A loss of 5.5% was found for phenylalanine after 24 h of hydrolysis under nitrogen. It is suggested that the results could be applied to animal proteins from tissue of similar composition but not to analysis of protein from plant materials.
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002645:Chickens; D002852:Chromatography, Ion Exchange; D006868:Hydrolysis; D010057:Oviducts / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
D000596:Amino Acids; D011506:Proteins