Attempt to isolate antierythrocyte antibodies by the method of affinity chromatography. I. IgM--complete antibodies.
It follows from the results of the present study that erythrocyte stromata can serve as a suitable insoluble immunosorbent of complete antibodies. When using optimum conditions, such as laboratory incubation timperature, suitable buffers for both washing the column prior to absorption and for elution, it is possible to obtain, by means of the chromatographic column with 0.5 ml of erythrocyte stromata suspended in 40 g of silica glass beads (ballotini), fractions devoid of complete antibodies and subsequently to obtain the latter in purified form by elution. In complete anti antibodies, the elution yield amounted to an average of 35% of the initial amount of antibodies. Although the elution yield is not high and non-specific absorption has been demonstrated, the method can be recommended for the isolation of complete antibodies and thus for the production of monospecific sera because it is easy, rapid and inexpensive due to the regeneration ability of the mentioned columns and the relatively long duration of the immunoabsorption capacity of the incorporated erythrocyte stromata.
D000042:Absorption; D002021:Buffers; D002846:Chromatography, Affinity; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D007164:Immunosorbents; D007518:Isoantibodies / Q000302:isolation & purification*; D013696:Temperature
D002021:Buffers; D007075:Immunoglobulin M; D007164:Immunosorbents; D007518:Isoantibodies