Selective inhibition of tubulin synthesis by amiprophos methyl during flagellar regeneration in Chlamydomonas reinhardi.
Amiprophos methyl (APM) is a strong, readily reversible and highly selective inhibitor of tubulin synthesis in Chlamydomonas reinhardi. The extensive induction of tubulin synthesis that accompanies flagellar regeneration in this organism is prevented by 3 to 10 micrometerAPM. When applied after induction has begun, APM causes a rapid cessation of tubulin synthesis. Translation studies in vitro indicate that the lack of tubulin production in APM-treated cells is not due to a direct inhibition of tubulin messenger RNA translation but rather to a selective depletion of tubulin messenger RNA.
D002696:Chlamydomonas / Q000648:ultrastructure; D003864:Depression, Chemical; D005260:Female; D005407:Flagella / Q000502:physiology / Q000648:ultrastructure; D006023:Glycoproteins / Q000096:biosynthesis*; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012038:Regeneration / Q000187:drug effects; D014404:Tubulin / Q000096:biosynthesis*; D015053:Zygote / Q000378:metabolism
D006023:Glycoproteins; D009946:Organothiophosphorus Compounds; D014404:Tubulin