Production and characterization of antibody against aflatoxin M1.
Antibody against aflatoxin M1 was obtained after immunization of rabbits with bovine serum albumin-afla M1 oxime conjugate. The antibody has greatest binding efficiency for afla M1, and was less efficient for afla B1. Cross-reaction of antibody with aflatoxin Q1, aflatoxicol, and aflatoxin B2a was weak. Aflatoxin B2, G1, and G2 and afla B1-guanine adducts showed almost no cross-reaction with the antibody. The sensitivity of the binding assay for aflatoxin M1 detection is in the range of 1-10 ng per assay. Detailed methods for the preparation of the conjugate, production of immune serum, and methods for antibody determination are described.
D000348:Aflatoxins / Q000276:immunology*; D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D011817:Rabbits / Q000276:immunology; D012709:Serum Albumin
D000348:Aflatoxins; D000906:Antibodies; D012709:Serum Albumin