Immunochemical studies of 5-bromodeoxyuridine.
Specific antibodies for BrdUrd and IdUrd have been produced and characterized by immunochemical studies. Studies as determined by hapten-inhibition showed a cross reactivity of less than 0.001% to thymidine, and no cross reactivity to bromodeoxycytidine, uridine or methyl cytosine. The equilibrium constant determination for anti-BrdU and IdUrd was 4.2 x 10(8)M-1. Unfractionated antiserum, produced under the conditions herein described, can be employed for the specific cytological detection of DNA replication.
D000818:Animals; D001973:Bromodeoxyuridine / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D003429:Cross Reactions; D004247:DNA / Q000096:biosynthesis; D005620:Freund's Adjuvant; D007114:Immunization; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013997:Time Factors
D012710:Serum Albumin, Bovine; D004247:DNA; D005620:Freund's Adjuvant; D001973:Bromodeoxyuridine