Separation of two forms of HeLa DNA polymerase alpha with different binding affinity to DNA.
Two forms of DNA polymerase alpha were separated from HeLa cells by DEAE-cellulose column chromatography and affinity chromatography using a DNA-cellulose column. They were eluted from a DEAE-cellulose column at 0.22M KCl (P-I) and 0.24M KCl (P-II). Upon cell fractionation between cytosol and nuclei, P-I was recovered from nuclei and P-II from cytosol. P-I was found to have a higher binding affinity to DNA than P-II by DNA-cellulose column chromatography.
D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D003600:Cytosol / Q000201:enzymology; D004247:DNA / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification*; D006367:HeLa Cells; D011485:Protein Binding; D012995:Solubility
D004247:DNA; D004259:DNA-Directed DNA Polymerase