[Obtaining ribosome crystals in homogenates].
Chick embryos are homogenized in order to analyse ribosome crystallization. Ribosome crystallization has been induced by hypothermic treatment in chick embryos homogenate. Tetramers and crystals were produced by gradually inducing the temperature over a span of 10 h to 4 degrees C. It has been observed that the concentration of KCl in the buffer is a critical point. It is suggested that the nuclear fraction is engaged in ribosome crystallization.
D000818:Animals; D002642:Chick Embryo; D003460:Crystallization; D008854:Microscopy, Electron; D011189:Potassium Chloride; D012270:Ribosomes / Q000648:ultrastructure*
D011189:Potassium Chloride
Ottenimento di cristalli ribosomiali in omogenato.