Detection of concanavalin A receptors by affinity to peroxidase and iron dextran by scanning and transmission electron microscopy and x-ray microanalysis.
A method of cell surface mapping has been developed on spermatozoa. Concanavalin A binding sites have been simultaneously revelaed both by peroxidase DAB reaction and by iron dextran coupling. The areas are examined in scanning and transmission electron microscopes and submitted to electron probe X-ray microanalysis.
D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000648:ultrastructure; D004577:Electron Probe Microanalysis; D007505:Iron-Dextran Complex / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010544:Peroxidases / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D011952:Receptors, Concanavalin A / Q000032:analysis / Q000378:metabolism; D011955:Receptors, Drug / Q000032:analysis; D013094:Spermatozoa / Q000648:ultrastructure*; D014965:X-Rays
D000345:Affinity Labels; D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D007505:Iron-Dextran Complex; D010544:Peroxidases