[The influence of drugs on the purine salvage metabolism in human blood cells (author's transl)].
The influence of 1-phenyl-2-(p-hydroxyphenyl)-3,5-dioxo-4n-butylpyrazolidine-monohydrate (oxyphenbutazone) and N-(alpha,alpha,alpha-trifluoro-m-tolyl)-anthranilic acid (flufenamic acid) on the purine salvage pathway of erythrocytes and lymphocytes following incubation of heparinized whole blood with the drugs was investigated. As described in earlier experiments in vitro with enzymes derived from cell homogenates, the enzyme activities of whole cells were also decreased. Lymphocytes exhibited a reduction of purine-phosphoribosyltransferase (purine-PRT) activities (adenine-, guanine- and hypoxanthine-PRT) of about 20%. The effect on the enzyme activities in red blood cells was different. The A-PRT-activity was decreased to about 33%, the GH-PRT on the other hand, to about 10%. The possible significance of these results in vivo is discussed.
D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005439:Flufenamic Acid / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D010113:Oxyphenbutazone / Q000494:pharmacology*; D011687:Purines / Q000097:blood*
D011687:Purines; D005439:Flufenamic Acid; D010113:Oxyphenbutazone
Die medikamentöse Beeinflussung des Purin-Wiederverwertungsstoffwechsels in menschlichen Blutzellen.