Modified bases characterized in intact DNA by mass-analyzed ion kinetic energy spectrometry.
Pyrolysis of DNA into a chemical ionization source yields protonated bases and other base-containing ions. Kinetic energy spectra allow the characterization of the bases 5-methylcytosine and 1-methyladenine from underivatized salmon sperm DNA. Isomeric bases are distinguishable with this technique.
D000818:Animals; D004247:DNA / Q000032:analysis*; D003838:Deoxyadenine Nucleotides / Q000032:analysis; D003845:Deoxycytosine Nucleotides / Q000032:analysis; D007536:Isomerism; D008297:Male; D013058:Mass Spectrometry; D008745:Methylation; D012474:Salmon; D013094:Spermatozoa / Q000032:analysis
D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D004247:DNA