Localization of concanavalin A binding sites on the cell membrane of Herpetomonas samuelpessoai: influence of growth conditions.
Herpetomonas samuelpessoai agglutinates when incubated with concanavalin A (Con A). Agglutination involved binding of Con A to specific receptors as it was inhibited by alpha-methyl-D-mannoside. The pattern and the intensity of the agglutination as well as the localization of Con A-binding sites were influenced by the composition of the growth medium, growth temperature and time of incubation. Con A-binding sites were detected by using the Con A-horseradish peroxidase-diaminobenzidine technique.
D000371:Agglutination; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003208:Concanavalin A / Q000276:immunology; D003470:Culture Media; D056890:Eukaryota / Q000254:growth & development / Q000276:immunology / Q000648:ultrastructure*; D011952:Receptors, Concanavalin A / Q000032:analysis*; D011955:Receptors, Drug / Q000032:analysis*
D003470:Culture Media; D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D003208:Concanavalin A