Tumor induction with the N'-acetyl derivative of 4-hydroxymethyl-phenylhydrazine, a metabolite of agaritine of Agaricus bisporus.
N'-Acetyl-4-(hydroxymethyl)phenylhydrazine was administered as a 0.0625% solution in drinking water continuously for the life span of Swiss mice, from 6 weeks of age. Compared to that in untreated controls, in treated animals the lung tumor incidence rose from 15 to 34% in females and 22 to 48% in males, whereas the incidence of blood vessel tumors increased from 8 to 32% in females and from 5 to 30% in males. Histopathologically, the tumors were classified as adenomas and adenocarcinomas of the lungs and angiomas and angiosarcomas of the blood vessels. The commonly eaten mushroom Agaricus bisporus contains beta-N-[gamma-L(+)-glutamyl]-4-hydroxymethylphenylhydrazine, which under certain conditions yields 4-hydroxymethylphenylhydrazine and L-glutamic acid. Since 4-hydroxymethylphenylhydrazine is relatively unstable, its acetyl derivative was synthesized for this study. The possible environmental significance of the findings is discussed.
D000230:Adenocarcinoma / Q000139:chemically induced; D000236:Adenoma / Q000139:chemically induced; D000818:Animals; D001487:Basidiomycota / Q000378:metabolism*; D001808:Blood Vessels; D002273:Carcinogens; D005260:Female; D005971:Glutamates / Q000378:metabolism; D006391:Hemangioma / Q000139:chemically induced; D006394:Hemangiosarcoma / Q000139:chemically induced; D008175:Lung Neoplasms / Q000139:chemically induced*; D008297:Male; D051379:Mice; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009383:Neoplasms, Vascular Tissue / Q000139:chemically induced*; D010659:Phenylhydrazines / Q000378:metabolism / Q000633:toxicity*
D002273:Carcinogens; D005971:Glutamates; D010659:Phenylhydrazines; C015654:agaritine