title: Alterations in cell free brain protein synthesis following ethanol withdrawal in physically dependent rats.
abstract: The effects of ethanol withdrawal were determined on cell free brain protein synthesis in physically dependent rats. Following the development of physical dependence, ethanol abstinence for 24 h resulted in decreased protein synthesis in cerebral tissue. The observed inhibition of [14C]leucine incorporation into protein was found to be reversible after 7 days of ethanol withdrawal. Although the ribosomes from control, ethanol-treated and ethanol-withdrawn animals were highly responsive to polyuridylic acid stimulation, the ribosomes from the control group consistently exhibited higher activity. The determination of protein content of the ribosomal fraction showed a significant increase following ethanol administration and was further enhanced by ethanol abstinence. The results suggest that ethanol-induced changes at the ribosomal level may result in defective association of mRNA causing depression of brain protein synthesis.
mesh_terms: D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002474:Cell-Free System; D006801:Humans; D007930:Leucine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010649:Phenylalanine / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012270:Ribosomes / Q000378:metabolism; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*; D013997:Time Factors
chemical_list: D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011072:Poly U; D010649:Phenylalanine; D007930:Leucine