Medium-chain fatty acid synthesis in lactating-rabbit mammary gland. Intracellular concentration and specificity of medium-chain acyl thioester hydrolase.
The concentration of medium-chain acyl thioester hydrolase and of fatty acid synthetase was determined by rocket immunoelectrophoresis in nine different particle-free supernatant fractions from lactating-rabbit mammary gland. The molar ratio of the hydrolase to fatty acid synthetase was 1.99 +/- 0.66 (mean +/- S.D.). A rate-limiting concentration of malonyl-CoA was required to ensure the predominant synthesis of medium-chain fatty acids when 2 mol of the hydrolase was added per mol of fatty acid synthetase. The interaction of the hydrolase with fatty acid synthetase was concentration-dependent, though an optimum concentration of hydrolase to synthetase could not be obtained. The lactating-rabbit mammary gland hydrolase altered the pattern of fatty acids synthesized by fatty acid synthetases prepared from cow, goat, sheep and rabbit lactating mammary glands, rabbit liver and cow adipose tissue.
D000818:Animals; D002417:Cattle; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D006041:Goats; D007122:Immunoelectrophoresis; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000201:enzymology; D007774:Lactation; D008316:Malonyl Coenzyme A / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000201:enzymology* / Q000378:metabolism; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D012756:Sheep; D013045:Species Specificity; D013379:Substrate Specificity; D013869:Thiolester Hydrolases / Q000378:metabolism*
D005227:Fatty Acids; D012710:Serum Albumin, Bovine; D008316:Malonyl Coenzyme A; D064429:Fatty Acid Synthases; D013869:Thiolester Hydrolases