title: RNA and protein synthetic patterns during germination of Polysphondylium pallidum microcysts.
abstract: During microcyst germination in the cellular slime mould Polysphondylium pallidum, an immediate rapid increase in the rate of protein synthesis ([3H]leucine incorporation) is observed within 15 min after the initiation of germination. The data, corrected for amino acid pool changes, reveal that the rate of protein synthesis reaches its peak at 1 1/2 h, after which it decreases. A low level of RNA synthesis ([3H]uridine incorporation) is observed after 1 h and this rate increases markedly after 2 h. Analysis of the RNA species shows a low level of synthesis of all ribosomal RNA's which begins between 1 and 2 h and increases after 2 h. The synthesis of a heterogeneously distributed, poly(A)-containing fraction of RNA (presumptive mRNA) is initiated some time after 2 h and the synthesis of a small molecular weight species in the 4-5S region is observed after 3 h. Thus, it seems that Polysphondylium microcysts show sequentially initiated synthesis of RNA during germination.
mesh_terms: D005656:Fungal Proteins / Q000096:biosynthesis*; D007700:Kinetics; D009235:Myxomycetes / Q000378:metabolism* / Q000502:physiology; D011061:Poly A; D012313:RNA / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis*
chemical_list: D005656:Fungal Proteins; D012335:RNA, Ribosomal; D011061:Poly A; D012313:RNA