Fibrinolysis induced by rat glioma cells. A mitosis-triggered process?
Experimentally induced brain glioma cell lines were studied in cell cultures for their fibrinolytic activity; simultaneously the mitotic index was determined. It was found that the most intense fibrinolytic activity of glioma cells coincides with mitotic waves. After 68 hr incubation of SD/G1 cells on a [125I]fibrin layer, [125I]fibrin digestion products were examined by gel chromatography. Hydroxy-urea and actinomycin D were used as inhibitors in cell cultures, investigating their effect on the expression of fibrinolysis. These studies reveal that [125I]fibrin digestion products are mainly low molecular weight (less than 12,000 daltons) compounds and that fibrinolysis is partially inhibited by the drugs added.
D000818:Animals; D001254:Astrocytoma / Q000032:analysis / Q000503:physiopathology; D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005342:Fibrinolysis; D005910:Glioma / Q000032:analysis / Q000503:physiopathology*; D006918:Hydroxyurea / Q000494:pharmacology; D008940:Mitotic Index; D009374:Neoplasms, Experimental / Q000503:physiopathology; D010960:Plasminogen Activators / Q000032:analysis; D051381:Rats; D009418:S100 Proteins / Q000032:analysis
D005338:Fibrin Fibrinogen Degradation Products; D009418:S100 Proteins; D003609:Dactinomycin; D010960:Plasminogen Activators; D006918:Hydroxyurea