title: Heterogeneous response of disseminated human ovarian cancers to cytostatics in vitro.
abstract: Cell suspensions from nine human ovarian primary cancers, their metastases and ascitic cells were treated in vitro with amethopterin and melphalan. Effects were measured by incorporation of H3-TdR or H3-UdR into the cells. There was significant heterogeneity of cytostatic effects on cells from the three sources in a given patient. Ascitic cells did nt represent a "mean" of the cancer cell clones. The implications of these findings should be considered if cytostatic in vitro prediction tests are used to guide cytostatic treatment of patients.
mesh_terms: D001202:Ascitic Fluid / Q000166:cytology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008558:Melphalan / Q000494:pharmacology / Q000627:therapeutic use*; D008727:Methotrexate / Q000494:pharmacology / Q000627:therapeutic use*; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D010534:Peritoneal Neoplasms / Q000188:drug therapy / Q000556:secondary*
chemical_list: D008558:Melphalan; D008727:Methotrexate