Incorporation of a fatty acid containing a photosensitive group into lipids of cultured cardiac cells from chick embryo.
Radioactive 12-(4-azido-2-nitrophenoxy)-stearic acid (NAP-stearate) was synthetized; it behaves as a competitive inhibitor of long-chain fatty acids for the entry into cultured cardiac cells from chick embryo. After uptake, [3H] NAP-stearate was incorporated by an energy-dependent process into neutral and polar lipids. Photoactivation as a function of time leads to a covalent labelling of the cells: up to 31 per cent of the radioactivity was recovered in the 105 000 g cell pellet, mainly in proteins. These experiments show that fatty acids containing photosensitive groups would potentially allow to localize the proteins involved in the binding and/or in the transport of fatty acids.
D000818:Animals; D001386:Azides; D001667:Binding, Competitive; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D005227:Fatty Acids / Q000378:metabolism*; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis*; D009206:Myocardium / Q000378:metabolism*; D013229:Stearic Acids / Q000494:pharmacology
D001386:Azides; D005227:Fatty Acids; D008055:Lipids; D013229:Stearic Acids