Neonatal rat hepatocytes in primary tissue culture free from density-dependent regulation of growth.
Studies employing [3H]thymidine and radioautography as well as colchicine and Feulgen staining of DNA showed that up to 19-fold increases in the degree of cell crowding in vitro, i.e. from 1.45 to 27.55 X 10(4) cells per specimen, did not change the rates of entry into DNA synthesis and mitosis of cultivated primary neonatal rat hepatocytes.
D000818:Animals; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003260:Contact Inhibition; D004247:DNA / Q000096:biosynthesis; D008099:Liver / Q000166:cytology*; D008938:Mitosis; D051381:Rats
D004247:DNA; D003078:Colchicine