Common features of coated vesicles from dissimilar tissues: composition and structure.
Coated vesicles were purified and characterized from porcine brain and chicken oocyte. Electrophoresis on sodium dodecylsulphate (SDS) gels showed that coated vesicles from either source have three major proteins in common with apparent molecular weights of 180000, 120000, and 55000 Daltons. Negatively stained specimens from both sources appear to consist of a highly ordered array of short interconnected rods or ridges of material on the exterior surface of a membrane vesicle. Coated vesicles purified from porcine brain and chicken oocyte have mean external diameters of 75.0 and 85.0 nm, respectively. In coated vesicles of the appropriate orientation, portions of the coat material appear to be organized into hexagons and pentagons. Based on the observed variability of size and apparent structure, it is postulated that the basic structural subunits of coated vesicles are the short rods or ridges of material observed on the exterior membrane vesicle surface. It is suggested that multiples of the subunits can be assembled into many arrangements to yield coated vesicles of different sizes and coat structure. It is also proposed that the structural subunit is a complex of 3 proteins of molecular weight of 180000, 120000 and 55000 Daltons.
D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002645:Chickens; D003593:Cytoplasm / Q000032:analysis / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008565:Membrane Proteins / Q000032:analysis; D008854:Microscopy, Electron; D008970:Molecular Weight; D009865:Oocytes / Q000648:ultrastructure; D013552:Swine
D008565:Membrane Proteins