Immunoaffinity purification of Schistosoma mansoni soluble egg antigens.
Schistosoma mansoni egg antigens were purified from a heterogeneous mixture of soluble egg antigens (crude SEA) with an immunoaffinity column that consisted of the specific anti-SEA antibodies contained in 16-week S. mansoni-infected mouse serum bound to Sepharose 4B. On sodium dodecyl sulfate (SDS) gel electrophoresis, the purified antigen fraction yielded at least eight bands staining with Coomassie blue and at least five bands staining with Coomaisse blue and at least five bands reacting with periodic acid-Schiff (PAS). All of the proteins in the antigenic fraction appear to contain carbohydrate residues. Upon immunoelectrophoresis the antigen yielded four precipitin arcs. The antigenic fraction isolated by means of the immunoaffinity column was then compared to various fractions obtained from concanavalin A (Con A) chromatography of SEA. The results of Ouchterlony immunodiffusion and immunoelectrophoresis indicate that the antigenic fraction isolated by immunoaffinity purification of SEA contains the major antigens found in the fractions obtained from Con A chromatography of SEA. The results of SDS gel electrophoresis indicate that the major PAS-reacting bands of the antigenic fraction isolated by immunoaffinity purification are found in the 3rd peak (bound fraction) resulting from Con A chromatography of SEA, whereas the major Coomaisse blue-staining band in the isolated antigenic fraction is found in the 2nd peak (unbound fraction) from Con A chromatography of SEA.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000941:Antigens / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D051379:Mice; D008808:Mice, Inbred CBA; D010063:Ovum / Q000276:immunology; D010455:Peptides / Q000302:isolation & purification; D010503:Periodic Acid-Schiff Reaction; D011817:Rabbits; D012550:Schistosoma mansoni / Q000276:immunology*; D012882:Skin Tests; D012995:Solubility
D000906:Antibodies; D000941:Antigens; D010455:Peptides; D003208:Concanavalin A