[Quantitative dansyl micromethod of amino acid analysis. Study of peptide maps of ribonuclease tryptic hydrolysate].
Some variants of the quantitative dansyl method in combination with thin-layer chromatography on polyamide plates have been compared, and principles of its optimization have been demonstrated. Sensitivity of the method is about 10(-11) mole, the error does not exceed 10%. Data are given on the analysis by the dansyl method of peptides of the tryptic hydrolysate of bovine pancreatic ribonuclease after their separation by peptide mapping procedure.
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds; D008722:Methods; D010179:Pancreas / Q000201:enzymology; D010446:Peptide Fragments / Q000032:analysis; D012260:Ribonucleases; D014357:Trypsin
D000596:Amino Acids; D003619:Dansyl Compounds; D010446:Peptide Fragments; D012260:Ribonucleases; D014357:Trypsin
Kolichestvennyĭ dansil'nyĭ mikrometod analiza aminokislot. issledovanie peptidnykh kart tripticheskogo gidrolizata ribonukleazy.