Isoelectric focusing pattern of acid hydrolases in cultured fibroblasts, leucocytes and cell-free amniotic fluid.
The activities of 9 acid hydrolases were determined in cell-free amniotic fluid, leucocytes and cultured fibroblasts using fluorogenic substrates. The specific activities of beta-glucosidase, alpha-fucosidase, beta-hexosaminidase, and arylsulphatase A and B were found to be in the same range in cell-free  amniotic fluid and in leucocyties. The isoenzyme pattern of these 5 hydrolases as well as that of acid phosphatase and alpha-mannosidase showed some similarities in all three specimens studied; the pattern of alpha- and beta-galactosidase obtained by isoelectric focusing was different in the 2 types of cells studied and in the cell-free amniotic fluid.
D000135:Acid Phosphatase / Q000378:metabolism; D000653:Amniotic Fluid / Q000201:enzymology*; D001192:Arylsulfatases / Q000378:metabolism; D002478:Cells, Cultured; D005347:Fibroblasts / Q000201:enzymology*; D005696:Galactosidases / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D007525:Isoelectric Focusing; D007962:Leukocytes / Q000201:enzymology*; D008361:Mannosidases / Q000378:metabolism; D012867:Skin; D005644:alpha-L-Fucosidase / Q000378:metabolism
D006867:Hydrolases; D000135:Acid Phosphatase; D001192:Arylsulfatases; D005696:Galactosidases; D005959:Glucosidases; D006596:Hexosaminidases; D008361:Mannosidases; D005644:alpha-L-Fucosidase