Genome analysis of Peromyscus (Rodentia, Cricetidae) VII. Localization of satellite DNA sequences and cytoplasmic poly(A) RNA sequences of P. eremicus on metaphase chromosomes.
A satellite DNA fraction from P. eremicus, having a buoyant density of 1.705 g/ml in neutral CsCl density gradients, was isolated. In situ hybridization experiments, using 3H-RNA complementary to this DNA fraction indicated that the short (heterochromatic) arms of most of the autosomes contained this sequence. Conversely, in situ hybridization using 3H-complementary DNA (cDNA) synthesized from the cytoplasmic poly (A) RNA of P. eremicus (comprising a substantial fraction of total messenger RNA) showed that the number of silver grains in the long arms (euchromatin) was significantly higher than that in the short arms. The X chromosomes showed a distinct localization pattern of both sequences.
D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000648:ultrastructure*; D003593:Cytoplasm / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004276:DNA, Satellite / Q000032:analysis*; D005260:Female; D006570:Heterochromatin / Q000032:analysis; D066298:In Vitro Techniques; D008677:Metaphase; D051379:Mice; D009693:Nucleic Acid Hybridization; D010542:Peromyscus / Q000235:genetics*; D011061:Poly A / Q000032:analysis*; D012313:RNA / Q000032:analysis*; D014960:X Chromosome / Q000032:analysis
D004276:DNA, Satellite; D006570:Heterochromatin; D011061:Poly A; D012313:RNA; D004247:DNA