Active enzyme sedimentation of pig heart fumarase.
Active band sedimentation studies of pig heart fumarase indicate that the enzyme is predominantly tetrameric at enzyme concentrations between 0.0125 and 0.25 mg/ml and at a fumarate concentration of 2.5 mM. At enzyme concentrations of 0.25--1.0 mg/ml and fumarate concentrations known to activate and inhibit the enzyme, the sedimentation band of fumarase becomes disperse and indicates the presence of polymers greater than tetramers.
D000818:Animals; D004789:Enzyme Activation; D005649:Fumarate Hydratase / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D005650:Fumarates / Q000494:pharmacology; D046911:Macromolecular Substances; D009206:Myocardium / Q000201:enzymology*; D013552:Swine; D014461:Ultracentrifugation
D005650:Fumarates; D046911:Macromolecular Substances; D005649:Fumarate Hydratase