title: Homozygous mouse embryos produced by microsurgery.
abstract: Homozygous mouse blastocysts have been produced following microsurgical removal of one pronucleus from the fertilized egg, diploidization in cytochalasin B, and culture in vitro. This combination of techniques should greatly shorten the time required to obtain homozygous strains of mice.
mesh_terms: D000818:Animals; D002467:Cell Nucleus; D002478:Cells, Cultured; D003571:Cytochalasin B; D005260:Female; D006720:Homozygote; D008722:Methods; D051379:Mice / Q000196:embryology*; D008866:Microsurgery; D010063:Ovum / Q000601:surgery*; D011003:Ploidies
chemical_list: D003571:Cytochalasin B