title: Preparation of ruthenium(II) and ruthenium(III) myoglobin and the reaction of dioxygen, and carbon monoxide, with ruthenium(II) myoglobin.
abstract: Ruthenium myoglobins have been prepared by the reconstitution of horse heart apomyoglobin with either ruthenium(II) or ruthenium(III) mesoporphyrin IX (MpIX) derivatives. The ruthenium(II) and -(III) myo globins (RuMb and RuMb+, respectively) contain one ruthenium porphyrin/heme binding site; the species are readily interconverted using dithionite for reduction and bromine for oxidation. RuMb binds carbon monoxide to give the known carbonyl complex. Reversible oxygenation occurs readily with protein-free RuII(MpIX) species in dimethylformamide, but RuMb in phosphate buffer is irreversibly oxidized by dioxygen to give RuMb+ via an outer sphere electron transfer mechanism.
mesh_terms: D000818:Animals; D002248:Carbon Monoxide; D003035:Cobalt; D004126:Dimethylformamide; D006736:Horses; D007700:Kinetics; D009206:Myocardium; D009211:Myoglobin; D010084:Oxidation-Reduction; D012428:Ruthenium; D013053:Spectrophotometry
chemical_list: D009211:Myoglobin; D003035:Cobalt; D002248:Carbon Monoxide; D012428:Ruthenium; D004126:Dimethylformamide