[Isolation and in vitro cultivation of tumor cells from human bronchial carcinoma transplanted to the nu/nu mouse].
Isolation of pure human tumor cell lines from surgical specimens is still a difficult task. As a result, only a limited number of such cell lines is available for experimental and diagnostic work. In contrast, isolation of malignant cells from human tumors serially transplanted in "nude" mice offers many advantages. The amount of tissue obtainable is theoretically unlimited and the percentage of viable cells is usually much larger than in human surgical specimens. However, no simple and reliable technique for isolation and purification of nu/nu-mouse-grown human tumor cells has been available up to now. This preliminary communication describes such a method. Its applicability has been successfully tested on 4 human bronchogenic carcinomas from which pure tumor cell lines could be established.
D000818:Animals; D002283:Carcinoma, Bronchogenic / Q000473:pathology*; D002469:Cell Separation; D046508:Culture Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation
Isolation und In-vitro-Kultivation von Tumorzellen aus menschlichen, in der nu/nu-Maus transplantierten Bronchuskarzinomen.