Effects on rat liver acetaldehyde dehydrogenases in vitro and in vivo by coprine, the disulfiram-like constituent of Coprinus atramentarius.
Coprine, the disulfiram-like constituent of the mushroom Coprinus atramentarius was found to inhibit the low-Km dehydrogenase in rat liver and to increase the acetaldehyde level in blood during ethanol metabolism in vivo. Coprine did not inhibit the low-Km enzyme in vitro, but the hydrolytic product of coprine, 1-aminocyclo-propanol, was a potent inhibitor both in vitro and in vivo. A rapid onset of inhibition was observed after administration of coprine and the inhibition was long-lasting. It is suggested that 1-aminocyclopropanol is responsible for the inhibition caused by coprine in vivo.
D000079:Acetaldehyde / Q000097:blood / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001487:Basidiomycota / Q000032:analysis; D003521:Cyclopropanes / Q000494:pharmacology; D000431:Ethanol / Q000378:metabolism*; D005260:Female; D005973:Glutamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology*; D051381:Rats; D013997:Time Factors
D003521:Cyclopropanes; D005973:Glutamine; C014423:coprine; D000431:Ethanol; D000445:Aldehyde Oxidoreductases; D000079:Acetaldehyde