Translation of endogenous message in embryonic chick erythroid cell polysomes in a cell-free protein-synthesizing system.
Polysomes from 5.5-day and 6.5-day embryonic chick erythroid cells contain messenger RNA (mRNA) which can be translated into products in a cell-free protein-synthesizing system. The products of the cell-free system coelectrophoresed with carrier globin chains from the cells from which the polysomal pellet was isolated. Wheat germ S30 fraction increases by 1.5--2.0 fold [3H] leucine incorporation into trichloroacetic acid-precipitable material directed by the endogenous mRNA on chick erythroid cell polysomes. The wheat germ probably provides a specific factor or factors absent in a shortage in the incubation medium.
D000818:Animals; D002474:Cell-Free System; D002642:Chick Embryo; D004912:Erythrocytes / Q000378:metabolism*; D005914:Globins / Q000096:biosynthesis; D007700:Kinetics; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000097:blood*
D012333:RNA, Messenger; D005914:Globins