A non-barbital buffer for immunoelectrophoresis and zone electrophoresis in agarose gels.
We describe buffer for both immunoelectrophoresis and zone electrophoresis procedures in agarose gels, Tricine [N-tris(hydroxymethyl)methylglycine] and Tris [2-amino-2-(hydroxymethyl)-1,3-propanediol] being the main components. This buffer eliminates the procurement cost and inventory control associated with formulation of barbital buffers. We compare it with other common buffer formulations.
D001462:Barbital; D002021:Buffers; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D014325:Tromethamine
D002021:Buffers; D014325:Tromethamine; D001462:Barbital; D005998:Glycine