Megakaryocyte colony culture using a liver cell conditioned medium.
Megakaryocyte colonies may now be grown in semi-solid agar, agarose, and plasma clot systems using a variety of conditioned media. These include mitogen activated mouse spleen cells, L-cells (fibroblasts), and a myelomonocytic cell line. To these is now added a conditioned medium from BRL-3A, a rat liver cell line. Megakaryocytes show full maturation to cytoplasmic fragmentation in this system. It is suggested that colony stimulating factor for megakaryocytes may come from a variety of sources; the relationship of any of these to the physiological regulator or regulators remains quite unknown.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D007739:L Cells / Q000166:cytology; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice; D011043:Pokeweed Mitogens / Q000494:pharmacology; D051381:Rats; D013154:Spleen / Q000166:cytology
D003470:Culture Media; D011043:Pokeweed Mitogens