title: Potency testing of pertussis immunoglobulins, relationship between neutralisation test and agglutination test.
abstract: Neutralisation tests were done with pertussis immunoglobulins and immunosera in which mice were immunised by intraperitoneal injection and challenged 4 hours later by intracerebral injection. The results of the neutralisation tests were compared with those of the agglutination tests done with the same preparations. For 2 pertussis immunoglobulins a correlation was found between the results of both tests, but generally no correlation exists between the agglutinating activity and the protective value of the preparations. There is a more than 100-fold difference in their relationship. Therefore a neutralisation test should be used for potency testing of pertussis immunoglobulins.
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D006801:Humans; D007106:Immune Sera; D007118:Immunoassay; D007136:Immunoglobulins / Q000032:analysis*; D051379:Mice / Q000276:immunology; D010567:Pertussis Vaccine / Q000032:analysis*; D011786:Quality Control; D012015:Reference Standards; D014917:Whooping Cough / Q000276:immunology*
chemical_list: D007106:Immune Sera; D007136:Immunoglobulins; D010567:Pertussis Vaccine