The development of chick spinal cord in tissue culture. II. Cultures of whole chick embryos.
By using whole-chick-embryo cultures followed by fragment cultures of spinal-cord primordia, it was possible to reproduce in vitro the whole process of neuronal development beginning with its initiation and continuing up to and including the maturation of neurons. Normal whole embryos were developed to Hamilton-Hamburger stages 17 and 18 by growing embryos from the primitive streak stage on large (28-mm) glass rings. The advantage of whole-embryo cultures is that development can be staged accurately, which is especially important during the early stages when morphogenesis progresses very rapidly. By using such accurately staged embryos and tritiated thymidine, we have determined that some postmitotic neuronal precursor cells appear in chick embryos as early as Hamburger-Hamilton stages 4 and 5, i.e. the definitive streak stages before the neural tube has formed.
D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo; D046508:Culture Techniques / Q000379:methods*; D009474:Neurons / Q000166:cytology; D013116:Spinal Cord / Q000196:embryology*