Step-wise cross-linking of pig heart lactate dehydrogenase by a heterobifunctional reagent.
Cross-linking of the essential cysteine-165 of pig heart lactate dehydrogenase (L-lactate: NAD+ oxidoreductase, EC 1.1.1.27) with lysine-179 was achieved by stepwise reaction with N-(4-azidocarbonyl-3-hydroxy-phenyl)-maleimide. These two residues are connected by kind of a hydrophobic channel which suggests that the essential cysteine is not modified via the active center as previously assumed.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D003545:Cysteine; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase; D008239:Lysine; D008301:Maleimides; D009206:Myocardium / Q000201:enzymology*; D010446:Peptide Fragments; D013552:Swine
D000596:Amino Acids; D007202:Indicators and Reagents; D008301:Maleimides; D010446:Peptide Fragments; D007770:L-Lactate Dehydrogenase; D008239:Lysine; D003545:Cysteine