[Separation of human X- and Y- chromosome-bearing spermatozoa by column chromatography (author's transl)].
Separation of X- and Y-chromosome-bearing spermatozoa has been attempted using Sephadex gel filtration. Fractions rich in X-chromosome-bearing spermatozoa were eluted in dependence on length of separation distance and on speed of elution. Spermatozoa remaining at the gel showed a higher percentage of Y-bodies compared with the untreated control. Sedimentation pattern and amount of eluted cells is influenced by eluation conditions.
D002469:Cell Separation / Q000379:methods; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D008297:Male; D012730:Sex Chromosomes; D013081:Sperm Motility; D014960:X Chromosome; D014998:Y Chromosome
Säulenchromatographische Trennung von humanen X- und Y-Spermien.