Anti-oestrogen action: uterine nuclear retention of the CI-628 anti-oestrogen receptor complex in vitro.
Experiments were performed in vitro which compared the nuclear retention of the anti-oestrogen receptor complex formed by CI-628 and the nuclear retention of the oestrogen receptor complex formed by oestradiol-17beta. Uterine nuclei retained the CI-628 receptor complex regardless of whether uterine incubations were carried out in the continued in fresh medium. However, for oestradiol-17beta there was a rapid decrease in the amount of receptor complex retained in the nucleus with time. When extractions using KCI were performed on nuclei after maximal accumulation of receptor complex by oestradiol-17beta or CI-628 the oestrogen receptor complex was found to have a higher relative nuclear affinity than did the CI-628 receptor complex. These studies suggest different nuclear binding sites for these complexes.
D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D009594:Nitromifene / Q000378:metabolism* / Q000494:pharmacology; D011759:Pyrrolidines / Q000378:metabolism*; D051381:Rats; D011960:Receptors, Estrogen / Q000187:drug effects; D014599:Uterus / Q000378:metabolism*
D011759:Pyrrolidines; D011960:Receptors, Estrogen; D004958:Estradiol; D009594:Nitromifene