Reactivity of antibodies to guanosine modified by the carcinogen N-acetoxy-N-2-acetylaminofluorene.
N-(guanosin-8-yl) acetylaminofluorene (Guo-AAF) was prepared by the reaction of N-acetoxy-N-2-acetylaminofluorene (AAAF) and guanosine. Antibodies to Guo-AAF were elicited in rabbits by immunization with bovine serum albumin-Guo-AAF conjugate. The antibodies were purified by affinity chromatography on a Sepharose-Guo-AAF column. The reactivity of these antibodies towards several ligands was studied by radioimmunoassay. The antibodies have the same affinity for double stranded DNA-AAF and single stranded DNA-AAF. Thus the geometry of the regions of DNA substituted by AAF residues is the same in native and denatured DNA. The affinity of the antibodies is smaller for DNA-AAF than for Guo-AAF. This can be due in part to the stacking of AAF residues with the adjacent bases as shown by the study of the interactions between the antibodies and AAF-oligonucleotides. The circular dichroism spectra of AAF-oligonucleotides bound to the antibodies are reported.
D000099:Acetoxyacetylaminofluorene; D000818:Animals; D000906:Antibodies; D002942:Circular Dichroism; D004247:DNA / Q000276:immunology; D005449:Fluorenes; D006151:Guanosine / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013056:Spectrophotometry, Ultraviolet
D000906:Antibodies; D005449:Fluorenes; D006151:Guanosine; D000099:Acetoxyacetylaminofluorene; D004247:DNA