Oncogenicity of a nude mouse cell line transformed by a human papovavirus.
Primary cultures of NIH nude mouse (nu/nu) kidney cells were transformed with a human papovavirus (MMV). The transformed cell line expressed T-antigen, and MMV DNA was found to be associated with the cell DNA. When NIH nu/nu mice were inoculated with the transformed cells, they developed tumors at the injection site but failed to generate detectable levels of T-antibody. A control group of nu/+ littermates rejected the tumor inoculum but mounted an antibody response to T-antigen. It was proposed that nude mouse cells may be a suitable system to test oncogenicity of in vitro transformed cells.
D000818:Animals; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002471:Cell Transformation, Neoplastic; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology; D027383:Papillomaviridae / Q000276:immunology; D027382:Polyomaviridae; D014184:Transplantation, Homologous
D000951:Antigens, Neoplasm; D000956:Antigens, Viral