Suppression of amphotericin B methyl ester induced growth stimulation in intraspecific mouse somatic cell hybrids.
Amphotericin B methyl ester (AME), the chemically modified derivative of amphotericin B, induced a concentration-dependent growth stimulatory effect on B82 mouse cells as indicated by increased 24- and 72-hour viable cell number, growth rate and DNA and RNA synthesis. In contrast, AME was not growth promoting toward RAG mouse cells or B82-RAG somatic cell hybrids, while hybrid cells exhibited the increased AME resistance pattern of B82 parental cells. A dissociation between the phenotypic expression of growth stimulation and polyene sensitivity was demonstrated in intraspecific mouse hybrids.
D000666:Amphotericin B / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002477:Cells / Q000187:drug effects* / Q000378:metabolism; D002860:Chromium Radioisotopes; D004247:DNA / Q000096:biosynthesis; D004351:Drug Resistance; D006822:Hybrid Cells; D051379:Mice; D012313:RNA / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014529:Uridine / Q000378:metabolism
D002860:Chromium Radioisotopes; D012313:RNA; D000666:Amphotericin B; D004247:DNA; D013936:Thymidine; D014529:Uridine