A multi-end point in vitro system for detection of new antitumor drugs.
By utilizing new types of producing microorganisms and isolating these on rather unusual growth media, we hope to produce new classes of antitumor drugs. In the detection system, we included the highly sensitive L1210 in vitro assay. But be requiring additional antimicrobial activity, we were able to eliminate rather early most of the previously known drugs from further work-up. The screening protocol was arranged so as to detect antimetabolites of a few rationally selected compounds.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification / Q000494:pharmacology; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001216:Asparagine / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002478:Cells, Cultured; D003470:Culture Media; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005973:Glutamine / Q000378:metabolism; D007939:Leukemia L1210 / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D000964:Antimetabolites, Antineoplastic; D000970:Antineoplastic Agents; D003470:Culture Media; D005973:Glutamine; D001216:Asparagine