The separation of X-and Y-spermatozoa with regard to the possible clinical application by means of artificial insemination.
With the sephadex gel-filtration method according to Steeno and Adimoelja (1974), X-spermatozoa have been successfully separated from the Y-spermatozoa in semen samples from 81 donors with the possibility of practical use with the aid of artificial insemination to promote pregnancy. A better quality of spermatozoa is obtained after this method of filtration, which will support these spermatozoa in having the potency of fertilization. There is indeed a decrease in concentration of spermatozoa after filtration, but the samples still fulfil the minimal concentration required in artificial insemination.
D002469:Cell Separation; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D007315:Insemination, Artificial; D008297:Male; D012730:Sex Chromosomes; D012732:Sex Determination Analysis; D013094:Spermatozoa / Q000648:ultrastructure*; D014960:X Chromosome; D014998:Y Chromosome