Morphologic changes during incubation of renal slices.
The progression of ultrastructural changes was studied during various conditions of incubation of rat and mice renal cortex slices. The kidneys were sliced with a Stadie-Riggs microtome and incubated in various media (tris(hydroxymethyl) aminomethane (Tris), Tris-proline, and Hanks), in different oxygenation conditions (O2-CO2, 6 liters per minute; N2, 0.5 liter per minute), at different temperatures (37 degrees C, 23 degrees C.) and different time intervals (0 to 120 minutes). The most conspicious changes appear to involve plasma membranes and mitochondria. Early changes (0 to 30 minutes) are mainly confined to mitochondria cristae and matrix; there is also light swelling of the endoplasmic reticulum. After 30 minutes of incubation, the majority of mitochondria have a condensed form: dense matrix and slight dilation of the cristae. These modifications are more accentuated at 75 minutes and many mitochondria are swollen and contain dense material. The modification of the membrane takes place at 30 minutes by vesiculation and/or a diffuse dilation of brush border and is followed by myelinization. The antiluminal membrane appears less sensitive but the same modification pattern appears after 75 minutes. These changes appear more slowly at 23 degrees C. than at 37 degrees C. Kinetic studies were also carried out confirming findings of various authors; cellular uptake and accumulation of 14C-glycine still take place after 2 hours of incubation. The kidney cortex slicing technique does not seem to be suitable for the study of absorption at the luminal membrane. Furthermore, our observations suggest that the generally accepted ultrastructure of some organelles does not correspond to their appearance in the noramlly functioning state in vivo.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003470:Culture Media; D046508:Culture Techniques; D005260:Female; D005998:Glycine / Q000378:metabolism; D007672:Kidney Cortex / Q000378:metabolism / Q000648:ultrastructure*; D051379:Mice; D008928:Mitochondria / Q000648:ultrastructure; D008933:Mitochondrial Swelling; D010100:Oxygen; D051381:Rats; D013045:Species Specificity; D013696:Temperature
D003470:Culture Media; D010100:Oxygen; D005998:Glycine