Rabbit beta-globin mRNA production in mouse L cells transformed with cloned rabbit beta-globin chromosomal DNA.
Mouse thymidine kinase-negative L cells were transformed with a cloned rabbit chromosomal beta-globin gene linked to the clone thymidine kinase gene of herpes simplex virus type 1. Most thymidine kinase-positive cell lines contained one or more copies of rabbit beta-globin DNA and produced up to 2,000 copies of rabbit beta-globin RNA per cell indistinguishable from its authentic counterpart. No mouse beta-globin mRNA was detected.
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004274:DNA, Recombinant; D005914:Globins / Q000235:genetics*; D007739:L Cells; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D012333:RNA, Messenger; D005914:Globins; D013937:Thymidine Kinase