Clinical use of immunoelectrophoresis of split ejaculates. I. Variation of patterns due to antisera.
As a preliminary investigation for the clinical application of immunoelectrophoresis of ejaculate in the diagnosis of male reproductive organ diseases, variations of immunoelectrophoretic patterns in normal subjects due to the difference of antigenicity between split ejaculates were demonstrated with rabbit antisera. Fresh samples and antisera against them gave larger numbers of and more intense precipitin lines than in stored samples. The precipitin lines developed by acid phosphatase were most remarkable between first portion of ejaculate (F1) and anti-F1, whereas the line caused by protein from the seminal vesicle was most prominent between the remainder of the ejaculate following F1 (F2) and anti-F2.
D000818:Animals; D000941:Antigens / Q000032:analysis; D004542:Ejaculation; D006801:Humans; D007122:Immunoelectrophoresis; D008297:Male; D008722:Methods; D011234:Precipitins; D011467:Prostate / Q000378:metabolism; D011817:Rabbits / Q000276:immunology; D012661:Semen / Q000032:analysis*; D012669:Seminal Vesicles / Q000378:metabolism; D013997:Time Factors
D000941:Antigens; D011234:Precipitins