Effects of estradiol-17beta in the male Xenopus laevis: isolation and translation of cytoplasmic messenger RNA populations.
Total Xenopus liver cytoplasmic RNA isolated following long-term estrogen administration (14 days) was fractioned using Sepharose 4B chromatography. One of the Sepharose 4B peaks was shown to contain RNA with a molecular weight reported for vitellogenin mRNA (approximately 34S). The presence of estrogen-induced vitellogenin mRNA in the peak 5 RNA was determined by translation of the RNA in the oocyte and analysis of the oocyte translational products by immunoprecipitation with anti-vitellogenin. Sepharose 4B peaks 2 and 3 were also observed to contain estrogen induced mRNA populations sedimenting between 9--18S. These findings suggest that Sepharose 4B chromatography might prove useful in separating different mRNA populations following estrogen-induced gene activation.
D000818:Animals; D003593:Cytoplasm / Q000032:analysis; D004527:Egg Proteins; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009865:Oocytes; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism; D014158:Transcription, Genetic; D014819:Vitellogenins; D014981:Xenopus
D004527:Egg Proteins; D012333:RNA, Messenger; D014819:Vitellogenins; D004958:Estradiol; D012313:RNA