Homogeneous cell populations from rabbit kidney cortex. Proximal, distal tubule, and renin-active cell isolated by free-flow electrophoresis.
A single-cell suspension has been prepared from rabbit kidney cortex by using a Ca-binding medium and gentle mechanical forces. The suspension was subjected to carrier-free electrophoresis, and several cell fractions were obtained. Proximal and distal tubule cell populations could be identified by their morphology. Renin-containing cells were located by means of radioimmunoassay. The morphology of the cells and their vitality (uridine incorporation) are discussed.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D004586:Electrophoresis; D007672:Kidney Cortex / Q000166:cytology* / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology*; D007686:Kidney Tubules, Distal / Q000166:cytology*; D007687:Kidney Tubules, Proximal / Q000166:cytology*; D011817:Rabbits; D012083:Renin / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D012083:Renin; D014529:Uridine