Bovine plasma as a reference standard for the factor VIII assay.
Definition of one unit of factor VIII procoagulant activity may be imprecise, for a number of reasons. Levels in individual normal plasmas differ sufficiently that small pools do not have equivalent activities. Large pools cannot be prepared without loss of activity because of the lability of factor VIII. Human factor VIII may not be stable at --20 C. Lyophilized standards may vary in activity because of difficulties in dissolving the materials. The authors have prepared standards that are stable at --20 C for years using beef plasma diluted in outdated human blood bank plasma. The level of activity of this working standard is verified by repeated assays of lyophilized national standards and of small pools of normal donor plasma. The standard is equally applicable to one- or two-stage assays for factor VIII.
D000818:Animals; D002417:Cattle / Q000097:blood; D005169:Factor VIII / Q000032:analysis*; D005615:Freezing; D006801:Humans; D010949:Plasma / Q000032:analysis; D012015:Reference Standards
D005169:Factor VIII