Immunochemical comparison of vitamin A binding proteins of rat.
Specific antibodies against cellular retinol-binding protein (CRBP), raised in rabbit, were detected by sucrose gradient centrifugation and gel filtration using tritium-labeled CRBP, prepared by reductive methylation. By means of radioimmunoassay, pure CRBP from liver and testis as well as CRBP present in a crude extract of liver were compared. All preparations showed identical immunoreactivity, suggesting CRBP is not tissue-specific. In contrast, two other vitamin A-binding proteins, cellular retinoic acid-binding protein, and serum retinol-binding protein, showed no cross-reaction in the radioimmunoassay.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002352:Carrier Proteins / Q000276:immunology; D008099:Liver / Q000032:analysis; D008297:Male; D009928:Organ Specificity; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D012177:Retinol-Binding Proteins / Q000097:blood / Q000276:immunology; D054840:Retinol-Binding Proteins, Cellular; D013737:Testis / Q000032:analysis; D014212:Tretinoin
D000906:Antibodies; D002352:Carrier Proteins; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D014212:Tretinoin