Warfarin-resistance genotype determination in the Norway rat, Rattus norvegicus.
The 2-stage determination is based on changes in blood coaggulation activity brought about both by the administration of warfarin in conjunction with vitamin K1 epoxide and by feeding a vitamin K-free diet for 4 days. When it was applied to laboratory-bred rats of known warfarin-resistance genotype, 35/35 homozygous susceptible, 44/44 homozygous resistant and 131/133 heterozygous rats were correctly classified. This method was equally effective in identifying the genotype of wild rats carrying the warfarin-resistance gene, Rw2. The procedure is rapid and accurate.
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D004351:Drug Resistance; D005260:Female; D006580:Genetic Carrier Screening; D005838:Genotype; D008297:Male; D010641:Phenotype; D051381:Rats / Q000097:blood* / Q000235:genetics; D014812:Vitamin K / Q000494:pharmacology; D014859:Warfarin / Q000494:pharmacology*
D014812:Vitamin K; D014859:Warfarin