A large-scale purification of phosphatidylethanolamine, lysophosphatidylethanolamine, and phosphatidylethanolamine, and phosphatidylcholine by high performance liquid chromatography: a partial resolution of molecular species.
Egg yolk phospholipids, on a 10 g scale, were resolved by high-performance liquid chromatography on an 8-m silica column with elution by a stepwise chloroformmethanol gradient into homogenous phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, and lysophosphatidylethanolamine fractions. Within these fractions, partial resolution on the basis of fatty acyl side chain composition was achieved.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004530:Egg Yolk; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D010713:Phosphatidylcholines / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000302:isolation & purification; D010716:Phosphatidylinositols / Q000302:isolation & purification; D010743:Phospholipids / Q000302:isolation & purification*
D005227:Fatty Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010743:Phospholipids