A gas chromatography-mass fragmentographic method for the assay of ajmaline and its monochloroacetyl ester.
A gas chromatograph-mass fragmentography method for simultaneous assay of 17-monochloroacetyl-ajmaline (MCAA) and its hydrolysis product, ajmaline, is described. Recovery of both compounds from whole blood averaged 71%. About 5% of MCAA was hydrolyzed during the assay procedure. The method was accurate and precise to within a few percent. It was suitable for assays of blood levels in the dog after an i.v. dose of 2 mg/kg or an oral dose of 5 mg/kg.
D000284:Administration, Oral; D000404:Ajmaline / Q000008:administration & dosage / Q000097:blood*; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D004285:Dogs; D004952:Esters; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006868:Hydrolysis
D004952:Esters; D000404:Ajmaline