Pyrimidine dimer excision in human cells and skin cancer.
We compared three methods for estimating the induction and removal of UV-induced pyrimidine dimers from the DNA of human fibroblasts. Results indicate that after UV doses of 5-20 J/m2, 50% of the dimers are removed by 24 hours. Almost complete excision can be observed if the cells are incubated for periods not less than 72 hours after a dose of 5 J/m2. After higher doses, it probably takes even longer for such complete removal to doses, it probably takes even longer for such complete removal to be seen.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004260:DNA Repair / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000378:metabolism; D005347:Fibroblasts; D006801:Humans; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D011740:Pyrimidine Dimers / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism*; D013997:Time Factors; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000378:metabolism*
D004273:DNA, Neoplasm; D011740:Pyrimidine Dimers