Interaction of DNA with guanidylated BPTI.
It was shown that guanidylated BPTI complexed with DNA stablized the native structure of the latter. It was found that hyperchromism values of DNA-guanidylated BPTI complexes, dialyzed against increasing and redialized against decreasing concentrations of NaCl or urea, amount ot ca 50% of hyperchromism values of free DNA or nondialyzed complexes. All three geometric forms of DNA were detected in DNA-guanidylated BPTI complexes subjected to the transient action of NaCl or urea.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D006146:Guanidines; D009691:Nucleic Acid Denaturation; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000378:metabolism* / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000378:metabolism*
D006146:Guanidines; D014361:Trypsin Inhibitors; D004247:DNA; D014360:Trypsin Inhibitor, Kunitz Soybean