African trypanosomes: cultivation of animal-infective Trypanosoma brucei in vitro.
Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37 degrees C. The organisms grown in this system were infective to mammalian hosts, retained the  morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces.
D000818:Animals; D000941:Antigens / Q000032:analysis; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D007739:L Cells / Q000469:parasitology; D008928:Mitochondria / Q000201:enzymology; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000254:growth & development* / Q000502:physiology
D000941:Antigens; D003470:Culture Media; D008058:Dihydrolipoamide Dehydrogenase