Employment of bis-intercalating dyes for the "in situ" study of DNA composition.
Spectrofluorometric characteristics of DNA-RO4 complexes have been studied in order to evaluate the possibility of employment of RO4 (a bis-intercalating dye) for the determination of the DNA base composition. The results have shown the usefulness of this compound for DNA with AT percentage between 30% and 60%. RO4 has been also applied to the study of the chromosome banding mechanism.
D000609:Aminoacridines; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002875:Chromosomes / Q000032:analysis; D004247:DNA; D004269:DNA, Bacterial; D006651:Histocytochemistry; D007364:Intercalating Agents; D007700:Kinetics; D010944:Plants; D011089:Polydeoxyribonucleotides; D011687:Purines / Q000032:analysis*; D011743:Pyrimidines / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013950:Thymus Gland
D000609:Aminoacridines; D004269:DNA, Bacterial; D007364:Intercalating Agents; D011089:Polydeoxyribonucleotides; D011687:Purines; D011743:Pyrimidines; D004247:DNA