title: Fat emulsions with added free cholesterol or fatty acid cholesteryl esters. Studies on removal mechanisms in vivo and hydrolysis by lipoprotein lipase in vitro.
abstract: The fractional elimination rate of fat emulsions of soybean oil, emulsified with egg yolk phosphatides with 1% addition of cholesterol or various cholesteryl fatty acid esters, was studied in rabbits. The fractional removal rate k2%/min was the same after addition of free cholesterol or esters with fatty acids up to eight carbon esters. The k2 values were twice as high for emulsions with cholesteryl-stearate, three times higher with added cholesteryl-palmitate and four times higher when cholesteryl-linoleate was added. The triglyceride (TG) lipase activity was determined with human or rabbit post-heparin plasma and with purified bovine lipoprotein lipase. All these enzyme sources gave similar results. Addition of saturated cholesteryl esters did not affect the lipase activity, but addition of 1% cholesterol markedly decreased the lipase activity. Furthermore, addition of cholesteryl-linoleate and linolenate reduced post-heparin TG lipase activity.
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism*; D004655:Emulsions; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006868:Hydrolysis; D008071:Lipoprotein Lipase / Q000097:blood; D009821:Oils; D011817:Rabbits; D013025:Glycine max
chemical_list: D002788:Cholesterol Esters; D004041:Dietary Fats; D004655:Emulsions; D009821:Oils; D006493:Heparin; D002784:Cholesterol; D008071:Lipoprotein Lipase