Intrachromosomal gene mapping in man: the gene for tryptophyl-tRNA synthetase maps in region q21 leads to qter of chromosome 14.
A gene for tryptophanyl-tRNA synthetase (EC 6.1.1.2), the enzyme which attaches tryptophan to its tRNA, has previously been assigned to human chromosome 14 by analysis of man-mouse somatic cell hybrids. We report here a method for the electrophoretic separation of Chinese hamster and human tryptophanyl-tRNA synthetases and its application to a series of independently derived Chinese hamster-human hybrids in which part of the human chromosome 14 has been translocated to the human X chromosome. When this derivative der (X),t(X;14) (Xqter leads to Xp22::14q21 leads to 14qter) chromosome carrying the human gene for hypoxanthine-guanine phosphoribosyltransferase was selected for and against in cell hybrid lines by the appropriate selective conditions, the human tryptophanyl-tRNA synthetase activity was found to segregate concordantly. These results provide additional confirmation for the assignment of the tryptophanyl-tRNA synthetase gene to human chromosome 14 and define its intrachromosomal location in the region 14q21 leads to 14qter. Our findings indicate that the genes for tryptophanyl-tRNA synthetase and for ribosomal RNA are not closely linked on chromosome 14.
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D002874:Chromosome Mapping; D002901:Chromosomes, Human, 13-15; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D012335:RNA, Ribosomal / Q000235:genetics; D014178:Translocation, Genetic; D014369:Tryptophan-tRNA Ligase / Q000235:genetics*; D014960:X Chromosome
D012335:RNA, Ribosomal; D000604:Amino Acyl-tRNA Synthetases; D014369:Tryptophan-tRNA Ligase