Impairment by interferon of mitogen-induced DNA synthesis in chick lymphocytes.
Chick interferon preparations inhibit mitogen-induced DNA synthesis of chicken peripheral and spleen lymphocytes. Mock interferon preparations showed also some inhibition of mitogen-induced DNA synthesis. The degree of inhibition is concentration dependent but varies also with the relative time of addition of homologous interferon and mitogen. Addition of the interferon preparations 6 h before mitogen resulted in a stronger inhibition of DNA synthesis than if the interferon preparations and mitogen were added at the same time. The interferon preparations seem to affect the metabolism of lymphocytes in a mroe general way and not only the trigger mechanism of the mitogenic response since addition of interferon even 24 h after the mitogen inhibits the induced DNA synthesis.
D000818:Animals; D002645:Chickens; D003208:Concanavalin A; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D007372:Interferons / Q000494:pharmacology*; D037102:Lectins; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice
D037102:Lectins; D003208:Concanavalin A; D004247:DNA; D007372:Interferons