Isolation of a crystalline A14-(N-methylpyridinium) derivative of bovine insulin.
Reaction of insulin with 2-chloro- or 2-iodo-N-methylpyridinium iodide affords, amongst others, an insulin analogue modified at tha A14-tyrosine residue. Degradative procedures which pinpoint the exact position of modification are described. Highest yields of the derivative are obtained using the 2-iodo-N-methylpyridinium iodide as reagent. In the mouse convulsion assay this derivative retains 72% of its activity.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D002417:Cattle; D002918:Chymotrypsin; D003460:Crystallization; D007328:Insulin; D046911:Macromolecular Substances; D011485:Protein Binding; D011726:Pyridinium Compounds; D014443:Tyrosine
D000596:Amino Acids; D007328:Insulin; D046911:Macromolecular Substances; D011726:Pyridinium Compounds; D014443:Tyrosine; C014677:1-methylpyridinium; D002918:Chymotrypsin