Calcium-sensitivity of the microtubule reassembly system. Difference between crude brain extract and purified microtubular proteins.
Microtubule reassembly in crude extracts of porcine brain was inhibited by 10(-5) M Ca2+, wherease 10(-3) M Ca2+ was required for inhibition of the reassembly from purified microtubular proteins. This accounts for the apparent discrepancies between the results reported by other investigators. Furthermore, the Ca-sensitivity of the purified microtubular proteins was nearly completely recovered on addition of a fraction obtained from crude brain extract.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology*; D006023:Glycoproteins / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D013552:Swine; D014020:Tissue Extracts; D014404:Tubulin / Q000378:metabolism*
D006023:Glycoproteins; D014020:Tissue Extracts; D014404:Tubulin; D002118:Calcium