[The effect of silybin dihemisuccinate on cholesterol biosynthesis in rat liver homogenates (author's transl)].
The effect of silybin dihemisuccinate on the hepatic biosynthesis of cholesterol was studied by measuring the incorporation of [1-14C]-acetate and [2-14C]-mevalonate in the postmitochondrial supernatant of liver homogenates. 2. Under absolute in vitro conditions, silybin dihemisuccinate inhibits the biosynthesis of cholesterol in dependence on its concentration. With [1-14C]-acetate or [2-14C]-mevalonate the concentrations of silybin for 50% inhibition are 0.37 mM or 0.40 mM, resp. 3. 30 min after i.v. injection of 150.6 mg silybin dihemisuccinate/kg with both precursors the biosynthesis of cholesterol studied in vitro is diminished in comparison to controls. 60 min or 24 h after i.v. injection of silybin no effects on in vitro biosynthesis of cholesterol can be observed.
D000818:Animals; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000096:biosynthesis*; D005419:Flavonoids / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D012838:Silymarin / Q000008:administration & dosage / Q000494:pharmacology*
D002250:Carbon Radioisotopes; D005419:Flavonoids; D012838:Silymarin; D002784:Cholesterol
Die Wirkung von Silybin-dihemisuccinat auf die Cholesterinbiosynthese in Rattenleberhomogenaten.