[Remote changes in the course of mitosis in synchronized cultures of Chinese hamster cells following inhibition of RNA and protein synthesis].
The effect of inhibition of the synthesis of some types of RNA and proteins was determined in the synchronized culture of Chinese hamster cells at various stages of the interphase on the course of remote mitosis. Analysis of MI and some forms of pathological cell division showed that the action of different doses of AMD and pyromycin during the first part of the interphase provoked an identical effect--C-mitosis at the immediate and remote waves of cell division. Suppression of the synthesis of whole cell RNA and proteins during the second half of the interphase was accompanied by a metaphase cell delay with scattering chromosomes, this indicating derangement of the synthesis of the division spindle component. It is suggested that proteins (tubulins) and RNA participate in the organization of the division spindle during remote mitosis as a "reserve pool".
D000818:Animals; D002999:Clone Cells; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D003609:Dactinomycin / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects; D011506:Proteins / Q000502:physiology*; D011691:Puromycin / Q000494:pharmacology; D012313:RNA / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects
D011506:Proteins; D003609:Dactinomycin; D011691:Puromycin; D012313:RNA; D003078:Colchicine
Otdalennye izmeneniia techneniia mitoza v sinkhronizirovannoĭ kul'ture kletok kitaĭskogo khomiachka posle ingibirovaniia sinteza RNK i belkov.