Bioaffinity chromatography of thyrotropin using immobilized concanavalin A.
The behavior of crude preparations of whale and bovine thyrotropins was studied on an affinity column packed with concanavalin A-Sepharose. Only a small portion of the proteins applied was adsorbed to the column and eluted quantitatively with 0.5 M methyl-alpha-D-glucoside or -mannoside. Immunoreactive as well as hormonally active thyrotropin was recovered exclusively in the absorbed fraction. The usefulness of this chromatographic procedure for the group separation of pituitary glycoprotein hormones is discussed.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A; D013045:Species Specificity; D013972:Thyrotropin / Q000302:isolation & purification*; D014907:Whales
D003208:Concanavalin A; D013972:Thyrotropin