Calorigenic effects of noradrenaline and glucagon on white adipocytes in cold- and heat-acclimated rats.
Calorigenic actions of noradrenaline and glucagon on isolated epididymal fat cells from warm-acclimated controls, cold-acclimated and heat-acclimated rats were measured by the use of a twin-type conduction microcalorimeter. Both noradrenaline and glucagon stimulated heat production in isolated adipocytes maximally in doses of 1 microgram/ml and 10 microgram/ml, respectively. Maximal responsiveness of adipocytes per unit cell to noradrenaline was not influenced by cold acclimation, while it was reduced by heat and acclimation. Maximal response in total epididymal fat cells to noradrenaline was increased in cold acclimation and not changed in heat acclimation at increased numbers of adipocytes in both cold-acclimated and heat-acclimated animals. Maximal response per unit cell as well as per total epididymal fat cells to glucagon was increased in cold acclimation and reduced in heat acclimation. The present results indicate that the modified responses of target adipocytes to noradrenaline and glucagon are involved in the development of temperature acclimation.
D000222:Adaptation, Physiological; D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D001833:Body Temperature Regulation / Q000187:drug effects*; D003080:Cold Temperature; D004305:Dose-Response Relationship, Drug; D005934:Glucagon / Q000494:pharmacology*; D006358:Hot Temperature; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats
D005934:Glucagon; D009638:Norepinephrine