title: Proton transport by bacteriorhodopsin through an interface film.
abstract: Interface films of purple membrane and lipid containing spectroscopically intact and oriented bacteriorhodopsin have been used as a model system to study the function of this protein. Small positive charges in surface potential (less than 1 mV) are detected upon illumination of these films at the air-water interface. These photopotentials are not affected by overlaying the interface film with a thin layer (0.3 mm) of decane. However, they are dramatically increased when lipid soluble proton carriers FCCP or DNP are added to the decane. The polarity of the photopotential indicates that, in the light, positive charges are transported through the interface from the aqueous to the organic phase. The action spectrum of the photopotential is identical to the absorption spectrum of bacteriorhodopsin. Since bacteriorhodopsin molecules are oriented with their intracellular surface towards the aqueous subphase, the characteristics of the photopotential indicate that in the light bacteriorhodopsin translocates protons from its intracellular to its extracellular surface. The kinetics of the photopotential reveal that the rate and extent of proton transport are proportional both to the fraction of bacteriorhodopsin molecules excited and to the concentration of proton acceptor. The photopotentials result from changes in the ionic distribution across the decane-water interface and can be cancelled by lipid soluble anions.
mesh_terms: D000388:Air; D000473:Alkanes; D001436:Bacteriorhodopsins / Q000378:metabolism*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002338:Carotenoids / Q000378:metabolism*; D004140:Dinitrophenols / Q000494:pharmacology; D004579:Electron Transport; D006217:Halobacterium; D008027:Light; D008564:Membrane Potentials / Q000187:drug effects; D008567:Membranes, Artificial; D008954:Models, Biological; D010713:Phosphatidylcholines; D011522:Protons; D013025:Glycine max; D014867:Water
chemical_list: D000473:Alkanes; D004140:Dinitrophenols; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D011522:Protons; D014867:Water; D002338:Carotenoids; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D001436:Bacteriorhodopsins