title: Culture of cloned cells from the mouse preputial gland tumor ESR 586.
abstract: Cells from the mouse preputial gland tumor ESR 586 have been cultured and cloned. Trypsin-ethylenediaminetetraacetate was used to obtain single cells. The cells are grown in a modified CMRL-1415 medium supplemented with 10% fetal calf serum. Clones tend to fall into two classes: Class 1, those that undergo morphological differentiation in liquid medium to form round bodies filled with lipid vaculoes; and Class 2, those that do not differentiate. Preliminary studies on the control of differentiation in Class 1 clones suggest that a minimum cell density is required before differentiation takes place. Analysis of the lipids from differentiating and nondifferentiating clones reveals the presence of sebaceous-type lipids in differentiating clones only. No requirement for testosterone was found for these cells.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008297:Male; D051379:Mice; D009374:Neoplasms, Experimental / Q000032:analysis / Q000473:pathology; D010412:Penile Neoplasms / Q000032:analysis / Q000473:pathology*; D012626:Sebaceous Gland Neoplasms / Q000032:analysis / Q000473:pathology*; D013739:Testosterone / Q000494:pharmacology
chemical_list: D008055:Lipids; D013739:Testosterone