[Analysis of the biorhythm frequency of bordered enterocytes during normal and inverted feeding regimens].
The study was performed during the period close to equinox in 236 male mice C57 Bl strain, sacrificed at equal intervals during 5 days. In paraffin sections, 5--6 mcm thick, stained after Dominici-Kedrovsky, with additional application of PAS-reaction and alcian blue, the cell height and width in the small intestine border enterocyte zone were determined. Calculation of autocorrelative function with the following determination of spectral density, infradian, circadian and ultradian biorhythmical components were estimated, as well as their changes under the influence of feeding regime inversion. At evening feeding, the greatest width of the brush border coinsides the beginning and the end of the digestion period. The width of the dark apical zone, where mitochondria are concentrated, synchronously changes at the end of digestion with the brush border, and then- with the cell basal zone. At morning feeding, by the degree of circadian phase change, the most liable are the indices reflecting organoid status, which are closely involved in absorbtion processes. Ultradian rhythm produces, on the background of circadian changes, additional raisis and abatements. Spectral composition, under inadequate feeding, is demonstrated to become more complex, mainly at the expense of ultradian rhythms, that speaks of their importance for the processes of adaptive rhythmical rearrangement.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002940:Circadian Rhythm; D004247:DNA / Q000096:biosynthesis; D004435:Eating; D005260:Female; D007413:Intestinal Mucosa / Q000166:cytology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010507:Periodicity; D011247:Pregnancy; D013057:Spectrum Analysis
D004247:DNA
Analiz chastoty bioritmov sostoianiia kaemchatykh énterotsitov pri adekvatnom i invertirovannom kormlenii.