[Metabolism and pharmacokinetics of piprozoline in rat, dog and man. 2nd Communication: Determination of metabolic profiles with the aid of an HPLC-radioactivity detection system following direct injection of urine and bile onto a reversed-phase column (author's transl)].
This paper describes the determination of metabolic profiles of 14C marked ethyl (Z-3-ethyl-4-oxo-5-piperidino-thiazolidin-2-ylidene) acetate (piprozoline, Gö 919, Probilin) in rat, dog and human urine and rat bile with the aid of an HPLC-radioactivity detector system following the direct injection of large quantities onto an analytical C-18 column. Metabolite 1 and two glucuronic acid conjugates of metabolite I were determined quantitatively, isolated and identified. Following HPLC separation, the specific detection of radioactivity permits the determination of metabolic profiles without prior clean-up so that unstable metabolites can also be analyzed.
D000328:Adult; D000818:Animals; D001646:Bile / Q000378:metabolism; D002756:Cholagogues and Choleretics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D005965:Glucuronates / Q000652:urine; D006801:Humans; D008297:Male; D010880:Piperidines / Q000378:metabolism; D051381:Rats; D013844:Thiazoles / Q000378:metabolism*
D002756:Cholagogues and Choleretics; D005965:Glucuronates; D010880:Piperidines; D013844:Thiazoles
Metabolismus und Pharmakokinetik von Piprozolin bei Ratte, Hund und Mensch. 2. Mitteilung: Erstellung von Metabolitenprofilen mit Hilfe der Kopplung HPLC-Radioaktivitätsdetektor nach direkter Injektion von Urin und Galle auf eine Reversed-Phase-Säule