Uptake of radiolabeled glucose analogues by organotypic cerebellar cultures.
Permeability of brain cells to radiolabeled glucose analogues with sucrose or inulin or L-glucose was studied in the living, intact state of the organotypic cerebellar cultures. In vitro uptake of 3H 3-O-methyl D-glucose and 3H 2-deoxy-D-glucose exhibited saturation  kinetics with increasing substrate concentrations. Each of the labeled sugars uptake could be self-inhibited in the presence of unlabeled (cold) substrate and cross-inhibited with cold glucose or xylose or phlorizin present in the incubating medium. The uptake was Na-independent and stereo-specific for D-form.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001812:Blood-Brain Barrier; D002531:Cerebellum / Q000378:metabolism*; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D009924:Organ Culture Techniques; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D013237:Stereoisomerism; D013395:Sucrose / Q000378:metabolism
D003837:Deoxy Sugars; D008757:Methylglucosides; D008759:Methylglycosides; D013395:Sucrose; D003847:Deoxyglucose; D012964:Sodium; D005947:Glucose