Macromolecular synthesis in cells infected by frog virus 3. VI. Frog virus 3 replication is dependent on the cell nucleus.
Previous evidence indicated that frog virus 3 (FV3), an icosahedral DNA virus, replicates exclusively in the cytoplasm. However, data presented here demonstrate that FV3 does not replicate in UV-irradiated or enuleated chicken embryo or BSC-1 cells and that virus-specific DNA synthesis is not initiated in such cells. Primary transcription was not detected in infected enucleated cells. These results demonstrate that a functional nucleus is essential for FV3 replication.
D000818:Animals; D001001:Anura / Q000382:microbiology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000382:microbiology / Q000528:radiation effects; D004267:DNA Viruses / Q000254:growth & development* / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis; D014466:Ultraviolet Rays; D014779:Virus Replication
D004279:DNA, Viral; D012367:RNA, Viral