Improved method for the purification of hog cholera virus grown in tissue culture.
A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved by rate zonal centrifugation in a 7--35 per cent sucrose gradient. This procedure permits a recovery of approximately 40 per cent of viral infectivity. A specific infectivity of about 2 X 10(7) PFU/microgram of protein was achieved. An apparent density d=1.13 g/ml in sucrose and a Sw20 value of about 150 was determined for purified HC virions.
D000818:Animals; D002460:Cell Line; D006691:Classical Swine Fever / Q000382:microbiology; D005466:Fluorocarbons; D012328:RNA Viruses / Q000254:growth & development / Q000302:isolation & purification*; D013552:Swine; D014461:Ultracentrifugation
D005466:Fluorocarbons