Uptake of fatty acids by cultured cardiac cells from chick embryo: evidence for a facilitation process without energy dependence.
Cultured heart cells from chick embryo accumulate fatty acids up to 50 fold at a steady-state level under defined conditions [ref.1]. Studies of fatty acid uptake as a function of different cellular parameters (intracellular ATP and pH, membrane potential and electrochemical gradients for monovalent and divalent cations) show the lack of effect of these factors. The rate of uptake is temperature-dependent. The maximum velocity V is affected with no change in the Km value of the saturable component; the activation energies were found to be 35.5 kJ.mol-1 for palmitate and 42 kJ.mol-1 for oleate. The results are in favour of a facilitation process which leads to an accumulation of fatty acids without energy dependence. The accumulation of fatty acids could be due to their association to intracellular membraneous and/or cytosolic components.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003486:Cyanides / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D007461:Iodoacetates / Q000494:pharmacology; D007700:Kinetics; D008744:Methylamines / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D009829:Oleic Acids / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology; D010169:Palmitic Acids / Q000378:metabolism; D013816:Thermodynamics
D000085:Acetates; D003486:Cyanides; D004140:Dinitrophenols; D005227:Fatty Acids; D007461:Iodoacetates; D008744:Methylamines; D009829:Oleic Acids; D009840:Oligomycins; D010169:Palmitic Acids