Resolution of small G1 and G2 populations of L1210 leukemia by flow microfluorometric analysis aided by velocity sedimentation.
L1210 leukemic cells grown in vitro were subjected to kinetic analysis using a flow microfluorometer. A single broad peak was found for the DNA content distribution if unfractionated cells were used; prior fractionation using lg velocity sedimentation allowed the separation of small peaks with smaller (G1) and larger (G2) DNA contents from the dominant S phase peak with intermediate DNA content.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004273:DNA, Neoplasm / Q000032:analysis; D005470:Fluorometry; D066298:In Vitro Techniques; D007700:Kinetics; D007939:Leukemia L1210 / Q000032:analysis*
D004273:DNA, Neoplasm