title: [Interaction of human creatine phosphokinase isoenzymes with rabbit antibodies and their Fab-fragments].
abstract: The interaction of human creatine phosphokinase isoenzymes with rabbit antibodies and their Fab has been studied. It has been shown that Fab of the antibodies against MM or BB isoenzymes preserve high specificity of intact antibodies and the ability to inhibit creatine kinase isoenzymes. Differences between antibodies and their Fab have been found to exist with respect to the kinetics of binding with homologous isoenzymes: the rate of the complex formation for Fab is significantly higher. The interaction of creatine kinase isoenzymes with intact antibodies and their Fab is not affected by the addition of creatine kinase substrates. The antibodies against MM and BB isoenzymes have been used to study the individual properties of each subunit of the M- and B-type in a hybrid dimer MB. It has been shown that such properties of these subunits as the Michaelis constants, pH dependence and inhibition by homologous antibodies are identical to those of non-hybrid MM and BB isoenzymes, respectively.
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D003402:Creatine Kinase / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007527:Isoenzymes / Q000276:immunology; D011817:Rabbits / Q000276:immunology
chemical_list: D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007527:Isoenzymes; D003402:Creatine Kinase
vernacular_title: Vzaimodeĭstvie izofermentov kreatinkinazy cheloveka s antitelami krolinka i kkh FAV-fragentami.