[Immunodepressive activity of the different fractions of the antilymphocyte serum obtained after separation on DEAE-Sephadex].
Three protein fractions were obtained after the separation of the antilymphocytic sera (ALS). The first and the second ones chiefly consisted of gammaglobulins, and the third fraction contained the rest serum proteins. The second fraction possessed the greatest lymphocytotoxic activity in vitro, and also immunodepressive activity in vivo; administration of this fraction to mice caused depression of antibody-producing cells in the spleen to 9.1 +/- 2.8 (90 +/- 28.9 antibody-producing cells per 1 X 10(6) cells in control). The first fraction possessed a much lesser immunodepressive activity, and the third one practically failed to depress the immune response.
D000818:Animals; D000921:Antibody-Producing Cells / Q000187:drug effects; D000961:Antilymphocyte Serum / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D007166:Immunosuppressive Agents / Q000302:isolation & purification* / Q000494:pharmacology; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D011817:Rabbits; D013154:Spleen / Q000166:cytology
D000961:Antilymphocyte Serum; D007166:Immunosuppressive Agents
Immunodepressivnaia aktivnost' razlichnykh fraktsiĭ antilimfotsitarnoĭ syvorotki, poluchennykh posle razdeleniia na DEAE-sefadekse.