The effect of magnesiumaspartate on preservation of the rat liver.
1. Isolated rat livers reperfused with an oxygenated isotonic Mg-aspartate or saline solution and stored 1 h at 36 degrees C, 4 h at 22 degrees C or 12 h at 6 degrees C were reperfused at 37 degrees C 150 min with a Krebs-Henseleit solution containing bovine albumin and erthrocytes gased with a 95% O2-5% CO2 gas mixture. 2. Light- and electronmicroscopic studies revealed minor changes after storage, whereas in the reperfused livers a focal distribution of well preserved and disintegrated hepatocytes was observed. 3. During storage the sum of adenine nucleotides in general decreased markedly paralleled by a significant rise of the lactate/pyruvate and hydroxybutyrate/acetoacetate ratios and pronounced breakdown of glycogen. Oxidative phosphorylation was resumed upon reperfusion resulting in de novo synthesis of ATP and ADP. The lactatelpyruvate and hydroxybutyrate/acetoacetate ratios normalized, whereas a resynthesis of glycogen was missing. 4. No conclusive evidence of a protective Mg-effect as observed in induced cardiac arrest has been obtained, probably due to structural and metabolic differences between the two organs and microcirculatory disorders as has been demonstrated by measurements of oxygen uptake using the multiwire electrode.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives*; D006019:Glycolysis / Q000187:drug effects; D007657:Ketone Bodies / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D011195:Potassium Magnesium Aspartate / Q000494:pharmacology*; D051381:Rats; D014022:Tissue Survival / Q000187:drug effects
D000227:Adenine Nucleotides; D007657:Ketone Bodies; D008112:Liver Glycogen; D001224:Aspartic Acid; D011195:Potassium Magnesium Aspartate