[Histoenzymatic reactions in the cells of the reticuloendothelial system of the liver and spleen and hepatocytes in acute Morfamquat dichloride poisoning].
Histoenzymatic changes in cells of the reticulo-endothelial system of liver and spleen and in hepatocytes were studied in the course of acute poisoning with Morfamquat dichloride, without and under the screen of vitamin E (to mice tissue restrictive respiration compound). This herbicide was given to mice in single, intraperitoneal injection of 100 mg/kg b.w. The choice of the enzymes was based on the so-called markers of the lysosomes structures of a cell (acid phosphatase-APh-EC. 3.1.3.2. and beta-glucuronidase-beta-GU-EC. 3.2.1.31) and Golgi apparatus (thiamin pyrophosphatase-TPP-EC. 3.5.99.2) It was stated that Morfamquat dichloride caused clear stimulation in the reticuloendothelial system of the organism, with occurrence of the high activity of APh and beta-GU in the lysosome and phagolysosome structures, growth of the lysosome membranes permeability and passing their content to cytoplasm. Furthermore, the increased activity of TPP-ase in Golgi apparatus hepatocytes of animals liver may point to disturbances of the pirogroniam decarboxylation and also to disturbances of the pentose-phosphate cycle. The application of vitamin E to mice for 5 days before the poisoning, protects animals distinctly against the toxic activity of the herbicide.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005966:Glucuronidase / Q000378:metabolism; D006540:Herbicides / Q000506:poisoning*; D008099:Liver / Q000473:pathology*; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D051379:Mice; D011726:Pyridinium Compounds / Q000506:poisoning*; D013154:Spleen / Q000473:pathology*; D013831:Thiamine; D013834:Thiamine Pyrophosphatase / Q000378:metabolism
D006540:Herbicides; D011726:Pyridinium Compounds; C008547:morfamquat; D000135:Acid Phosphatase; D005966:Glucuronidase; D013834:Thiamine Pyrophosphatase; D013831:Thiamine
Odczyny histoenzymatyczne w komórkach układu siateczkowo-śródbłonkowego watroby i śledziony oraz hepatocytach w przebiegu ostrego zatrucia Morfamquatem dichloride.