Characteristic proteins systhesized by suspensions of cells isolated from rat gastric mucosa.
The biochemical integrity of rat gastric mucosal cells after isolation with pronase was demonstrated by their synthesis of a wide range of proteins. Approximately 50 bands of pre-existing proteins were detectable by staining on SDS-polyacrylamide electrophoresis gels. Most bands appeared readiolabelled after incubating cells with 14C-leucine or 14C-proline and were detected by autoradiography and fluorography of the gels. Recent synthesis, indicated by the radioactivity of each band, was compared with historical synthesis indicated by the intensity of staining. Densitometric comparisons showed the relative specific radioactivity of certain bands, e.g., one of nominal molecular weight 25000, to be consistently higher than the integrated mean value.
D000818:Animals; D001345:Autoradiography; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005753:Gastric Mucosa / Q000378:metabolism*; D008970:Molecular Weight; D014176:Protein Biosynthesis; D051381:Rats