title: Purification and characterization of a lectin from the mushroom, Flammulina veltipes.
abstract: A lectin was purified to homogeneity from the mushroom, Flammulina veltipes, by zinc acetate treatment and CM-cellulose column chromatography. Its molecular weight was estimated to be 20,000 by gel filtration and polyacrylamide gel electrophoresis. The lectin does not contain carbohydrate, half-cystine, methionine, or histidine. On gel filtration sith Sepharose 6B in the presence of 6M guanidine-HCl, the purified lectin dissociated into two nonidentical subunits, FVA-L (molecular weight, 12,000) and FVA-S (8,000). The hemagglutinating activity was retained only in the FVA-L subunit. The lectin is mitogenic with respect to mouse spleen lymphocytes.
mesh_terms: D000363:Agaricales / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D006384:Hemagglutination; D037102:Lectins / Q000302:isolation & purification; D008213:Lymphocyte Activation; D051379:Mice; D008970:Molecular Weight; D011817:Rabbits; D013154:Spleen
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D037102:Lectins