Studies on the turnover of proteins of the rat erythrocyte membrane.
The membrane proteins of erythrocytes were labeled by injecting L-[14C]-leucine and later L-[3H]leucine into rats, the two injections being 31 days apart. Control animals received the two isotopic forms of L-leucine simultaneously. Deviations in labeling ratio from control patterns were found on sodium dodecyl sulfate-polyacrylamide gel electrophorograms in restricted regions suggestive of turnover or loss of a few small proteins from the membrane between the 31 days. Most of the ghost proteins show no turnover.
D000818:Animals; D002250:Carbon Radioisotopes; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D007553:Isotope Labeling; D007930:Leucine / Q000097:blood; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D051381:Rats; D013997:Time Factors; D014316:Tritium
D002250:Carbon Radioisotopes; D008565:Membrane Proteins; D014316:Tritium; D007930:Leucine