[Effect of actinomycin D and sibiromycin on 3H-thymidine incorporation into the early developmental stages of Nereis virens Sars].
The Nereis virens embryos at the stages of 2, 8, 16 and 32 blastomeres end of cleavage and beginning of rotation were placed in the actinomycin D or sibiromycin solutions and the effect of antibiotics on 3H-thymidine incorporation during cleavage, at the beginning of rotation and in trochophore was determined by means of autoradiography after careful washing the embryos off. Under the effect of actinomycin D the intensity of 3H-thymidine incorporation during cleavage decreased insignificantly, at the gastrula stage somewhat exceeded that in the control, and at the stages of trochophore formation decreased twice. At the later stages it approached the normal level. In the experiments with sibiromycin which proved to have more distinct inhibitory effect, the stage of trochophore formation was also found to be the most sensitive to the antibiotic.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001345:Autoradiography; D004247:DNA / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology*; D011077:Polychaeta / Q000196:embryology* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D003609:Dactinomycin; D004247:DNA; D013936:Thymidine
Vliianie aktinomitsina D i sibiromitsina na vkliuchenie 3H-timidina na raznykh stadiiakh razvitiia Nereis virens Sars.