Mutation assay in diploid human lymphoblasts: methodological aspects.
The protocol for a recently developed quantitative assay for mutation at the hgprt locus of human lymphoblasts is presented. Practical problems affecting ease of performance and reliability are discussed with the aim of making the assay available for assessment and possible use in other laboratories.
D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D004171:Diploidy; D005347:Fibroblasts; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D009152:Mutagenicity Tests