In vitro investigations on the effect of pilocarpine on the metabolism in pig lenses illustrated with some intermediate metabolites.
22 pairs of pig lenses were incubated in TCM 199 5 g, with pilocarpine 5 X 10(-4) M and 10(-2) M. 5 X 10(-4) M is the probable concentration in the aqueous during ordinary pilocarpine medication. The concentration of 1-lactate, pyruvate, RTP, RDP and AMP was determined in the lenses and the assimilation of glucose and the production of 1-lactate and pyruvate were measured. As far as pilocarpine 5 X 10(-4) M was concerned no significant differences were found, but pilocarpine 10(-2) M gave a significantly higher 1-lactate concentration in the lenses (118%) and increased the assimilation of glucose to 120% and also augmented the production of 1-lactate to 168% and the pyruvate to 184%, tending to show that pilocarpine 10(-2) M increased the glycolytic activity in the lenses. The concentrations of RTP, RDP and AMP were unchanged.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D005947:Glucose / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism*; D010862:Pilocarpine / Q000494:pharmacology*; D011773:Pyruvates / Q000378:metabolism; D012263:Ribonucleosides / Q000378:metabolism; D013552:Swine
D007773:Lactates; D011773:Pyruvates; D012263:Ribonucleosides; D010862:Pilocarpine; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D005947:Glucose