[Interaction between tryptophanyl-tRNA synthetase and tryptophan analogs with modified alpha-amino group].
Analogs of L-tryptophane with modified alpha-amino groups (substituted for hydrogen or keto-group, or acylated) are competitive reversible inhibitors of the aminoacylation of tRNNATrp catalyzed by tryptophanyl-tRNA synthetase from beef pancreas. Compounds lacking alpha-amino group are weakly bound to the enzyme, whereas the Ki values for N-acylated L-tryptophane derivates and for beta-indolylpyruvic acid are similar and approximately two orders of magnitude higher than the KM value for L-tryptophane.
D000604:Amino Acyl-tRNA Synthetases / Q000037:antagonists & inhibitors*; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D007700:Kinetics; D010179:Pancreas / Q000201:enzymology; D011485:Protein Binding; D013329:Structure-Activity Relationship; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000494:pharmacology; D014369:Tryptophan-tRNA Ligase / Q000037:antagonists & inhibitors*
D014364:Tryptophan; D000604:Amino Acyl-tRNA Synthetases; D014369:Tryptophan-tRNA Ligase
Vzaimodeĭstvie triptofanil-TRNK-sintetazy s modifitsirovannymi po a-aminogruppe analogami triptofana