Absence of erythroblastic islands in plasma clot culture and their possible reconstitution after clot lysis.
Ultrastructural studies of erythroid colonies derived from human peripheral blood and growing in plasma clot culture have confirmed the absence of a macrophage inside each colony of erythroblasts. However, when macrophages and erythroblasts were liberated from the semisolid media by clot lysis, these two types of cells rapidly acquired intimate contacts, suggesting the reconstitution of any erythroblastic island. The possible significance of this phenomenon is discussed.
D002450:Cell Communication; D002478:Cells, Cultured; D004900:Erythroblasts / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure*; D006801:Humans; D008264:Macrophages / Q000648:ultrastructure; D008854:Microscopy, Electron