In vitro metabolism of lidoflazine by rat and dog liver fractions.
Lidoflazine was metabolized very rapidly by 16000 g supernatant fractions of rat and dog liver. The rate and the extent of metabolism were considerably superior for rat liver. Lidoflazine metabolites were purified by extraction and thin-layer chromatography and identified by mass spectrometry. The main pathways of the in vitro metabolism by rat and dog liver fractions were the same. Oxidative N-dealkylation was the most important. An incubation of major metabolite 1-[4,4-bis(4-fluorophenyl)-butyl]piperazine, with rat liver fraction was performed. A hydroxylated metabolite and a ketopiperazine metabolite were detected only in the dog experiments.
D000818:Animals; D004285:Dogs; D005260:Female; D066298:In Vitro Techniques; D008013:Lidoflazine / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D013058:Mass Spectrometry; D010879:Piperazines / Q000378:metabolism*; D051381:Rats; D013045:Species Specificity; D013347:Subcellular Fractions / Q000378:metabolism
D010879:Piperazines; D008013:Lidoflazine