An improved gas-liquid chromatographic procedure for the determination of amitriptyline and nortriptyline levels in plasma using nitrogen-sensitive detectors.
An improved gas-liquid chromatographic procedure for the plasma level determination of amitriptyline and nortriptyline using nitrogen-sensitive detectors is described. Derivatization of the secondary amines using trifluoroacetic anhydride greatly improves the response and reproducibility of the assay. Plasma samples containing as little as 5 ng/ml of amitriptyline and nortriptyline can be assayed precisely and reproducibly, using protriptyline as internal reference standard.
D000639:Amitriptyline / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D009584:Nitrogen; D009661:Nortriptyline / Q000097:blood*; D012015:Reference Standards
D000639:Amitriptyline; D009661:Nortriptyline; D009584:Nitrogen