Design, synthesis and characterisation of a 34-residue polypeptide that interacts with nucleic acids.
Based on secondary structure prediction rules and model building a neutral artificial 34-residue polypeptide with potential nucleic acid-binding activity was synthesised. This peptide and its covalent dimer showed strong interaction with cytidine phosphates and single-stranded DNA. The dimer had considerable ribonuclease activity with high preference for cleavage at the 3'-end of C.
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000138:chemical synthesis*; D002942:Circular Dichroism; D003597:Cytosine Nucleotides / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism*; D046911:Macromolecular Substances; D010455:Peptides / Q000138:chemical synthesis; D012260:Ribonucleases / Q000378:metabolism; D013329:Structure-Activity Relationship
D002352:Carrier Proteins; D003597:Cytosine Nucleotides; D004277:DNA, Single-Stranded; D046911:Macromolecular Substances; D010455:Peptides; D012260:Ribonucleases