title: Effect of squamous metaplasia on infection of hamster trachea organ cultures with Mycoplasma pneumoniae.
abstract: An organ culture system for hamster trachea was developed for maintenance of the ciliated respiratory epithelium during periods of extended cultivation (i.e., greater than 20 days). Evaluation of five serum types showed that horse serum and fetal calf serum were best for the maintenance of epithelial ciliary activity and morphology. Rings that were opened on one side ("split rings") had the best maintenance of the ciliated epithelium as judged by the retention of ciliary activity and normal histological appearance after 3 to 4 weeks in culture. The in vitro induction of squamous metaplasia was achieved by cultivating explants in Waymouth MAB 87/3 (vitamin A-free) medium, without serum. This system allowed a direct comparison of the effects of Mycoplasma pneumoniae infection in two epithelial types, ciliated pseudostratified columnar and keratinizing squamous. Attachment of 14C-labeled mycoplasmas was more than twofold greater in the normal epithelium. Pretreatment of explants with neuraminidase decreased attachment for both squamous and pseudostratified epithelial surfaces to a similar basal level. Recovery of viable organisms from infected tissue of both epithelial types indicated that the organism titer remained essentially constant during the infection period, but was significantly higher for the pseudostratified ciliated epithelium. These results suggest that specific receptor sites for M. pneumoniae are markedly reduced by the induction of squamous metaplasia and, hence, appear to be specific for the normal respiratory surface containing goblet cells and pseudostratified, ciliated epithelial cells.
mesh_terms: D000818:Animals; D001769:Blood; D002923:Cilia; D006224:Cricetinae; D003470:Culture Media; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D008679:Metaplasia; D009174:Mycoplasma / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology; D009924:Organ Culture Techniques; D014132:Trachea / Q000382:microbiology / Q000473:pathology*
chemical_list: D003470:Culture Media; D009439:Neuraminidase