title: Survival of mouse embryos after freezing in medium containing dimethylsulphoxide and yolk extract.
abstract: The ability of the cryoprotective solution containing dimethylsulphoxide in combination with yolk extract (DYG 550) to protect mouse embryos at ultra low temperatures is reported. Of the embryos of 46.1% frozen to -196 C at various rates formed blastocysts in vitro compared to 41.2% and 23.2% survivals protected by 1M dimethylsulphoxide and 1M glycerol.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004530:Egg Yolk; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D005615:Freezing; D066298:In Vitro Techniques; D051379:Mice; D011309:Preservation, Biological; D013696:Temperature
chemical_list: D004121:Dimethyl Sulfoxide