Estrogen withdrawal in chick oviduct. Evidence for continued expression of active unique genes using an "expressed" DNA probe.
We have analyzed the effect of estrogen on the kinds of unique DNA sequences which are transcriptionally expressed in chick oviduct with an "expressed" DNA probe. Steady-state nRNA in estrogen-stimulated chick oviduct represents about 25% of the complexity of total chick unique DNA. To purify this expressed DNA fraction, chick unique DNA was isolated, nick-translated, and hybridized to chemically mercurated oviduct nRNA (Hg-nRNA); the resulting hybrids were bound to sulfhydryl-Sepharose, and DNA was selectively recovered by thermal elution in formamide buffer. To compare the sequence homology between nRNAs isolated from oviduct before or up to 6 days after estrogen withdrawal, trace amounts of expressed DNA derived from estrogen-stimulated oviduct were hybridized in RNA-excess reactions. All nRNAs hybridized with equal efficiency. Furthermore, hybridization of expressed DNA to nRNA mixtures showed that nRNA from nonwithdrawn and withdrawn oviduct contained a similar set of unique sequences. The data indicate that, at most, only a small percentage (0--5%) of transcriptionally active unique DNA sequences are shut down when estrogen is removed from the circulation.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D004247:DNA / Q000378:metabolism*; D004355:Drug Stability; D005260:Female; D005796:Genes; D006589:Hexestrol / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D010057:Oviducts / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D013375:Substance Withdrawal Syndrome; D013696:Temperature; D014158:Transcription, Genetic / Q000187:drug effects*
D006589:Hexestrol; D012313:RNA; D004247:DNA