[Effect of cycloheximide on the ultrastructure and several metabolic processes of the cells of an SPEV culture. II].
The action of cyclohexamide (in doses of 1 and 10 mkg/ml in the course of 24 hours) on porcine embryo kidney cells in culture is accompanied by a powerful suppression of protein and DNA synthesis, mitotic activity, a weaker suppression of RNA synthesis, a lowering of the activity of succinate-, lactate- and alpha-glycerophosphate-dehydrogenase, which leads to disorders in the ultrastructure of the cells. After incubation of cells in a fresh medium (in the course of 18, 24 hours) there occurs a total restoration of the ultrastructure of the nuclei, granular endoplasmatic reticulum, mitochondria, due to the repairing and strong intensification of synthetic processes, respiration and glycolysis, mitotic activity of the cells.
D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000648:ultrastructure; D003513:Cycloheximide / Q000506:poisoning*; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D008938:Mitosis; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D013552:Swine / Q000196:embryology; D013997:Time Factors
D002843:Chromatin; D012313:RNA; D004247:DNA; D003513:Cycloheximide
Deĭstvie tsiklogeksimida na ul'trastrukturu i nekotorye metabolicheskie protsessy kletok kul'tury SPEV. Soobshchenie II.