Synthesis of heterogeneous nuclear RNA in full-grown oocytes of Xenopus laevis (Daudin).
At various times following injection of either 3H-GTP or 32PO4 into full-grown (stage 6) Xenopus laevis oocytes, RNA has been extracted and fractionated on polyacrylamide gels. Based on size, base composition and incorporation data, we have defined  the kinetics of synthesis and accumulation of ribosomal RNA (40S, 28S, 18S), heterogeneous RNA of high molecular weight (greater than 40S) and heterogeneous RNA migrating with molecular weights of from 4S to 40S. Nuclear isolations have been performed to determine the cellular distribution of these classes of RNA as a function of time. Evidence is presented which shows that stage 6 oocytes synthesize RNA which by virtue of its size, base composition, rapid turnover and nuclear location is equivalent to the heterogeneous nuclear RNA observed in somatic cells. In addition, the data suggest synthesis of a class of nuclear RNA with a half-life of several hours. A small fraction (5%) of the nuclear RNA is stable, enters the cytoplasm and may represent RNA  added to the stockpile of maternal transcripts known to be present in stage 6 oocytes.
D000818:Animals; D003596:Cytosine / Q000032:analysis; D005260:Female; D006147:Guanine / Q000032:analysis; D006207:Half-Life; D007700:Kinetics; D008970:Molecular Weight; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D012313:RNA / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D012335:RNA, Ribosomal / Q000096:biosynthesis; D014542:Uridine Monophosphate / Q000032:analysis; D014981:Xenopus
D012335:RNA, Ribosomal; D006147:Guanine; D012313:RNA; D003596:Cytosine; D014542:Uridine Monophosphate