Nuclear ribonucleoprotein particles in the cellular slime mold Dictyostelium discoideum.
The nuclear ribonucleoprotein (RNP) particles containing rapidly labeled RNA were isolated from interphase cells of the cellular slime mold Dictyostelium discoideum and characterized. The size of the isolated RNP particles was small (10S to 50S) in comparison with that of nuclear RNP particles found in higher eukaryotes. These small RNP particles do not seem to be artifacts due to degradation during the preparation of nuclear extracts. The rapidly labeled RNA of the nuclear RNP particles was heterogeneous in size and a considerable amount contained polyadenylic acid sequences. Synthesis of RNA in the nuclear RNP particles was resistant to a relatively high concentration of actinomycin D. The protein component of the RNP particle consists of at least four proteins with molecular weights of 80,000, 66,000, 60,000, and 42,000. Thus it is suggested that almost all of the nuclear RNP particles containing rapidly labeled RNA in interphase cells are RNP complexes consisting of Heterogeneous nuclear RNA and several protein species.
D002467:Cell Nucleus; D003609:Dactinomycin / Q000494:pharmacology; D004023:Dictyostelium; D008970:Molecular Weight; D009235:Myxomycetes; D009698:Nucleoproteins / Q000378:metabolism*; D011061:Poly A; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D012261:Ribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D014498:Uracil / Q000378:metabolism
D009698:Nucleoproteins; D012261:Ribonucleoproteins; D003609:Dactinomycin; D011061:Poly A; D014498:Uracil; D012313:RNA