A novel procedure for obtaining denuded sea urchin eggs and observations on the role of the vitelline layer in sperm reception and egg activation.
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D004229:Dithiothreitol; D004527:Egg Proteins / Q000096:biosynthesis; D005260:Female; D005306:Fertilization; D008722:Methods; D010063:Ovum / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D010312:Parthenogenesis; D010447:Peptide Hydrolases; D012617:Sea Urchins; D014633:Valine / Q000378:metabolism; D014817:Vitelline Membrane / Q000502:physiology*; D015053:Zygote / Q000378:metabolism
D004527:Egg Proteins; D000643:Ammonium Chloride; D010447:Peptide Hydrolases; D014633:Valine; D004229:Dithiothreitol