Prostaglandin production by methylcholanthrene-transformed mouse BALB/3T3. Requirement for protein synthesis.
Stimulation of prostaglandin synthesis in transformed mouse fibroblasts by serum, thrombin, and bradykinin was blocked by actinomycin D and cycloheximide. These RNA and protein synthesis inhibitors did not affect prostaglandin synthetase in vitro or in vivo; nor did they affect the acylation of arachidonic acid into phospholipids. Serum-stimulated release of arachidonic acid and prostaglandins from [3H]arachidonic acid-labeled cells also was inhibited by actinomycin D and cycloheximide. RNA and protein synthesis appear to be required for expression of phospholipase activity; a prerequisite for prostaglandin synthesis by these cells.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001095:Arachidonic Acids / Q000378:metabolism; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004640:Emetine / Q000494:pharmacology; D007700:Kinetics; D008748:Methylcholanthrene / Q000494:pharmacology*; D051379:Mice; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D014176:Protein Biosynthesis / Q000187:drug effects*; D014105:Tosyl Compounds / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D000900:Anti-Bacterial Agents; D001095:Arachidonic Acids; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D014105:Tosyl Compounds; D003609:Dactinomycin; D008748:Methylcholanthrene; D003513:Cycloheximide; D004640:Emetine