Stable derivatives for the gas chromatographic determination of synthetic anabolic stilbene residues (diethylstilbestrol, dienestrol and hexestrol) in meat and organs of treated cattle in the sub-parts per billion (10(9)) level.
A method for the determination of diethylstilbestrol and the related compounds dienestrol and hexestrol residues in meat and organs of treated cattle is described. After extraction and clean-up, these synthetic estrogens are subjected to reaction with pentafluorobenzoyl chloride, which gives very stable perfluoro esters that are suitable for gas chromatographic determination using an electron-capture detector. With the careful clean-up and the very sensitive response of these derivatives, it is possible to reach a limit of detection in the sub-parts per billion (10(9)) range starting with only 5 g of sample.
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D004028:Dienestrol / Q000032:analysis*; D004054:Diethylstilbestrol / Q000032:analysis*; D004951:Esterification; D006589:Hexestrol / Q000032:analysis*; D013058:Mass Spectrometry; D008460:Meat / Q000032:analysis*; D008722:Methods; D010636:Phenols / Q000032:analysis*
D010636:Phenols; D006589:Hexestrol; D004054:Diethylstilbestrol; D004028:Dienestrol