Structural similarities of hog cholera virus with togaviruses.
Hog cholera virus grown in PK-15 cells was purified by centrifugation through a sucrose cushion followed by sucrose gradient centrifugation. Analysis of virus labeled externally with [3H]sodium borohydride on polyacrylamide gel electrophoresis revealed two glycoproteins, gp55 and gp46. A third structural polypeptide, p36, seems not to be glycosylated. The gp46 was also found in the virus-free supernatant of infected cells. It could be demonstrated by radioimmune precipitation of virus labeled with[35S]methionine that all three polypeptides are specific for hog cholera virions. Electron microscopically hog cholera virus appeared as a spherical particle with a diameter of 42 +/- 8 nm. The virus particles frequently displayed a fringe of projections with a length of about 6--8 nm. The similarities of hog cholera virus with Alphaviruses and Flaviviruses are discussed.
D000818:Animals; D001103:Arboviruses / Q000648:ultrastructure*; D002460:Cell Line; D006691:Classical Swine Fever / Q000382:microbiology*; D006023:Glycoproteins / Q000032:analysis; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D012328:RNA Viruses / Q000648:ultrastructure*; D011863:Radioimmunoassay; D013552:Swine; D014764:Viral Proteins / Q000032:analysis
D006023:Glycoproteins; D010455:Peptides; D014764:Viral Proteins