Studies on lectins. XLI. Isolation and characterization of a blood group B specific lectin from the role of the powan (Coregonus lavaretus maraena).
A B-specific lectin from the roe of the powan (Coregonus lavaretus maraena), a fish of the Salmonidae family, was isolated by affinity chromatography on O-alpha-D-galactosyl polyacrylamide gel. From 630 g of the lyophilized roe, 346 mg of pure lectin was obtained in a single isolation step. The lectin is electrophoretically homogeneous, its sedimentation coefficient s20,w is 2.9S and molecular weight 25 000. The molecular weight of the subunits estimated by electrophoresis in the presence of dodecyl sulfate is 27 000 for both reduced and nonreduced substance. The lectin contains a large amount of cysteine, has a small content of aromatic amino acids, 10.4% of neutral sugar and 0.145% of Zn. It agglutinates specifically human B-group erythrocytes; the agglutination is stimulated by Zn2+ and Mg2+ ions and partially inhibited by EDTA. The most efficient carbohydrate inhibitors are methyl alpha-L-rhamnoside (6 micrometer), L-rhamnose (12 micrometer) and raffinose (0.1 mM). The association constant of the complex lectin . L-rhamnose is KA = 9.5 . 10(2) M-1, as determined by fluorimetric titration.
D000017:ABO Blood-Group System; D000373:Agglutinins / Q000302:isolation & purification*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000918:Antibody Specificity; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006388:Hemagglutinins / Q000302:isolation & purification*; D010063:Ovum / Q000032:analysis*; D012210:Rhamnose / Q000494:pharmacology; D012487:Salmonidae
D000017:ABO Blood-Group System; D000373:Agglutinins; D000596:Amino Acids; D006388:Hemagglutinins; D012210:Rhamnose