Purification and specificity of antibodies to inosine 5'-monophosphate.
Antibodies to inosine 5'-monophosphate elicited in rabbits by immunization with a conjugate of IMP (oxidized with periodate) and bovine serum albumin have been purified by affinity chromatography. By the use of two affinity columns, Sepharose-IMP and Sepharose-oligo(I), the antibodies have been fractionated into three fractions. By gel diffusion, the three fractions were found to react with the conjugates of bovine serum albumin and IMP, GMP and AMP respectively. The association constants for the binding of the Fab fragments purified on the Sepharose-oligo(I) column and several haptens have been deduced from fluorescence experiments. It is shown that the base and the phosphate group play an important part in the binding of IMP to Fab fragments. No reaction has been found between the antibodies and poly(I).poly(C) by gel diffusion. However, the antibodies interact with poly(I).poly(C) since they decrease the thermal stability of poly(I).poly(C).
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D004355:Drug Stability; D006241:Haptens; D006358:Hot Temperature; D007140:Immunoglobulin Fab Fragments; D007292:Inosine Nucleotides / Q000276:immunology*; D011070:Poly I-C; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000906:Antibodies; D006241:Haptens; D007140:Immunoglobulin Fab Fragments; D007292:Inosine Nucleotides; D011070:Poly I-C