title: On enzymatic clotting processes. I. Kinetics of enzyme-triggered coagulation reactions.
abstract: The kinetics of enzymatic clotting reactions such as the clotting of blood or milk, is analyzed. The appearance of a lag phase in the clotting is shown to be due to the difference in reaction order of enzymatic production and flocculation. The weight-average particle weight of the product formed is found to be a quadratic function of the reaction time. The condition for the clotting time is t square root of ksV/2 = C, where t is the clotting time, ks the flocculation rate constant, V the maximum rate of enzymatic product formation and C a constant. In agreement with this result double-logarithmic plots of t versus enzyme dilution are always observed to be linear over a wide range of enzyme concentrations. No statistical evidence could be produced for the widely held belief that the clotting time should be inversely proportional to the enzyme concentration. Varying exponents of the latter in its relation to the clotting time are discussed in terms of von Smoluchowski's theory of the slow coagulation of colloidal particles.
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D004798:Enzymes / Q000378:metabolism*; D005427:Flocculation; D007700:Kinetics; D008433:Mathematics; D008892:Milk / Q000378:metabolism*; D008970:Molecular Weight; D009391:Nephelometry and Turbidimetry
chemical_list: D004798:Enzymes