Studies on the biosynthetic pathway of human P erythrocyte antigen using genetic complementation tests between fibroblasts from rare p and Pk phenotype donors.
Using the indirect immunofluorescence technique, P antigen is not detected on skin fibroblasts from Pk1, Pk2 or p donors. P antigen is reexpressed on polykaryon cells obtained by fusion of Pk x p fibroblasts. On the contrary, P antigen is not expressed on polykaryon cells from Pk x Pk or p x p fusion. All these data suggest that in Pk and p fibroblasts, at least two distinct genes are modified, one in the p phenotype, and the other in the Pk phenotype. The two Pk phenotypes studied seem to be homogeneous from a genetic point of view, as do the five p phenotypes.
D000818:Animals; D001789:Blood Group Antigens; D002459:Cell Fusion; D004996:Ethidium; D005347:Fibroblasts / Q000276:immunology*; D005816:Genetic Complementation Test; D006041:Goats / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007519:Isoantigens / Q000096:biosynthesis*; D010127:P Blood-Group System; D010641:Phenotype; D012867:Skin / Q000166:cytology
D001789:Blood Group Antigens; D007106:Immune Sera; D007519:Isoantigens; D010127:P Blood-Group System; D004996:Ethidium