[Suppression of the damaging effect of amphotericin B on dog kidney lysosomes by amigluracyl].
The effect of amphotericin B and its combination with amigluracyl on the dog kidney lyzosomes was studied in vitro. It was found that on incubation of the lyzosomes with the antibiotic in a concentration of 1 gamma/ml the latter stimulated liberation of proteases from them. At the same time, when the lyzosomes were exposed to amphotericin B in combination with amigluracyl, a significant decrease in the rise of the proteolytic activity in the incubation medium due to the antibiotic was observed. It was found that the combined use of amphotericin B with amigluracyl resulted in an intensive inhibition of the enzyme activity; The data are indicative of the fact that amigluracyl decreases the damaging effect of the antibiotic on the dog kidney lyzosomes.
D000666:Amphotericin B / Q000037:antagonists & inhibitors*; D000818:Animals; D002175:Candida / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004285:Dogs; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D006657:Histones / Q000378:metabolism; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000648:ultrastructure*; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D008566:Membranes / Q000187:drug effects; D010447:Peptide Hydrolases / Q000378:metabolism; D010539:Permeability; D014498:Uracil / Q000031:analogs & derivatives* / Q000494:pharmacology
D002843:Chromatin; D006657:Histones; D014498:Uracil; D000666:Amphotericin B; D004247:DNA; D010447:Peptide Hydrolases; D005944:Glucosamine
Podavlenie amigluratsilom povrezhdaiushchego deĭstviia amfoteritsina B na lizosomy pochek sobak.