Stereospecific antibodies to methadone. II. Synthesis of d- and 1-methadone antigens.
Diastereomeric methadols were prepared by reduction of the methadone enantiomers. Resulting methadols, after purification and characterization by mass spectrometry, were succinylated. The hemisuccinyl derivatives were conjugated with thyroglobulin via carbodiimide condensation. The conjugates of d- and of 1-methadol hemisuccinyl-thyroglobulin were employed as the antigens for immunization of white New Zealand rabbits in order to obtain specific anti d- and anti 1-methadone antisera.
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens; D008691:Methadone / Q000031:analogs & derivatives / Q000276:immunology*; D010084:Oxidation-Reduction; D011817:Rabbits / Q000276:immunology; D013237:Stereoisomerism; D013386:Succinates / Q000138:chemical synthesis; D013954:Thyroglobulin
D000941:Antigens; D013386:Succinates; D013954:Thyroglobulin; D008691:Methadone