Biosynthesis of the major human red cell sialoglycoprotein, glycophorin A, in a continuous cell line.
During biosynthesis of glycophorin A in K562 cells a precursor is rapidly transferred through the endoplasmic reticulum membrane with the COOH-terminal remaining in the cytoplasm. This is glycosylated within the cell and appears at the cell surface after about 30 min. The biosynthetic pathway resembles that described for viral membrane glycoproteins.
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D006021:Glycophorins / Q000096:biosynthesis*; D006801:Humans; D007942:Leukemia, Experimental; D008861:Microsomes / Q000378:metabolism; D008970:Molecular Weight; D011498:Protein Precursors / Q000378:metabolism; D012795:Sialoglycoproteins / Q000096:biosynthesis*
D006021:Glycophorins; D011498:Protein Precursors; D012795:Sialoglycoproteins