Detection of antiplatelet antibody in immune thrombocytopenic purpura.
Two methods have been developed for the detection of antiplatelet antibodies in immune thrombocytopenic purpura (ITP). The first, a competitive RIA, is highly sensitive, reproducible and allows quantitation of platelet associated immunoglobulins (PAI) and specifically adsorbed antiplatelet antibodies from serum of patients with ITP. The second procedure developed is a simple and rapid qualitative test, where antiplatelet antibodies are detected through the formation of rosettes of protein A producing Staphylococcus aureus with sensitized human platelets.
D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D001666:Binding Sites, Antibody; D001792:Blood Platelets / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007223:Infant; D008297:Male; D008875:Middle Aged; D011696:Purpura, Thrombocytopenic / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods*; D012397:Rosette Formation / Q000379:methods*; D013205:Staphylococcal Protein A / Q000276:immunology
D001323:Autoantibodies; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A