Biochemical and physiological studies of androgen-binding protein in the reproductive tract of the ram.
The electrophoretic mobility, effect of pronase, temperature stability, affinity constant and specificity of androgen-binding protein (ABP) were compared in rete testis fluid (RTF), cauda epididymal plasma (CEP) and seminal plasma (SP) of the ram in which the levels of ABP, dihydrotestosterone (DHT), total protein and the number of spermatozoa were also measured. The characteristics of the ABP appeared to be almost identical in all 3 fluids. ABP was highly concentrated in the cauda epididymidis although 50-75% of it was utilized or destroyed during transit through the epididymis. The levels of ABP were higher in the breeding season and positively correlated with DHT in RTF and SP. It is concluded that ABP might be responsible for the increase in DHT in the reproductive tract of the ram during the breeding season and that ABP in the SP might serve as a useful marker of Sertoli cell function in the ram.
D000727:Androgen-Binding Protein / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D013196:Dihydrotestosterone / Q000032:analysis; D004586:Electrophoresis; D004822:Epididymis / Q000032:analysis; D006358:Hot Temperature; D008297:Male; D011402:Pronase / Q000494:pharmacology; D011506:Proteins / Q000032:analysis; D012621:Seasons; D012661:Semen / Q000032:analysis; D013076:Sperm Count; D013737:Testis / Q000032:analysis*
D000727:Androgen-Binding Protein; D002352:Carrier Proteins; D011506:Proteins; D013196:Dihydrotestosterone; D011402:Pronase