Gel filtration of egg phosphatidylcholine vesicles.
The abilities of Sepharose 2B (Pharmacia), Controlled Pore Glass (Electro-Nucleonics) and Bio-Gel A150m (Bio-Rad) to purify small unilamellar vesicles prepared by sonication and the ethanol-injection methods were compared. The Bio-Gel causes complete aggregation of the sonicated vesicles and partial aggregation of the ethanol-injection vesicles. Both Sepharose and Controlled Pore Glass are acceptable for purifying vesicles from multilamellar liposomes; however, neither will separate the vesicles from sonication by-products which might be formed.
D002850:Chromatography, Gel / Q000379:methods; D004530:Egg Yolk; D005260:Female; D005782:Gels; D005898:Glass; D008081:Liposomes / Q000302:isolation & purification*; D010713:Phosphatidylcholines / Q000302:isolation & purification*; D012685:Sepharose
D005782:Gels; D008081:Liposomes; D010713:Phosphatidylcholines; D012685:Sepharose