title: Intestinal response of sheep to intraperitoneal immunization.
abstract: The aim of the present study was to develop an immunization procedure which centrally stimulated the IgA system of sheep with the release of antibody-containing cells (ACC) of the IgA class into intestinal lymph. It was found that intraperitoneal injection of ovalbumin resulted in a substantial output of ACC in intestinal lymph. ACC of the IgA class reached a peak 8--9 days after intraperitoneal injection when they comprised 1.4% of cells in lymph. ACC of the IgM and IgG1 classes comprised 3.5 and 2.9% of cells in lymph at this time. The output of ACC of the IgA, IgM and IgG1 classes in lymph at the peak of the response was respectively, 3.7 X 10(6), 9.8 X 10(6) and 8 X 10(6) cells/h. In marked contrast to rats, virtually no ACC appeared in intestinal lymph of sheep following intraduodenal infusion of ovalbumin in animals primed 2 weeks earlier by intraperitoneal injection of antigen in Freund's complete adjuvant (CFA).
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D004386:Duodenum / Q000276:immunology; D007114:Immunization; D007070:Immunoglobulin A / Q000096:biosynthesis; D007274:Injections, Intraperitoneal; D007422:Intestines / Q000276:immunology*; D007958:Leukocyte Count; D008196:Lymph / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D012756:Sheep / Q000276:immunology*
chemical_list: D007070:Immunoglobulin A; D010047:Ovalbumin