Studies of a large transformation-increased membrane protein in the BHK21 cell system.
We have recently described in BHK cells a plasma membrane protein of molecular weight 177,000, which is significantly increased in Hamster Sarcoma Virus-transformed cells (Lage-Davila, A. and Montagnier, L. (1977) Biochem. Biophys. Res. Commun. 79, 577--584). We present now a study of proteins from purified plasma membrane fractions in the same pair of clones. Solubilization conditions, cross-linking experiments, metabolic labelling and enzymatic radioiodination allow to characterize this 177,000 transformation-increased protein as an integral membrane glycoprotein partially exposed at the outer cell surface. Additional information on other membrane proteins in this system is also given.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002472:Cell Transformation, Viral; D003300:Copper; D006224:Cricetinae; D005944:Glucosamine / Q000378:metabolism; D007454:Iodides / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008623:Mercaptoethanol; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009858:Oncogenic Viruses; D010618:Phenanthrolines; D011092:Polyethylene Glycols
D007454:Iodides; D008565:Membrane Proteins; D010618:Phenanthrolines; D011092:Polyethylene Glycols; D008623:Mercaptoethanol; D003300:Copper; D008715:Methionine; D005944:Glucosamine