title: Plasma membrane alterations as a result of heat activation in Dictyostelium spores.
abstract: At the end of heat activation the distribution of spore plasma membrane particles between the two fracture faces (PF and EF) is drastically changed. While in dormant spores the particle number ratio of PF/EF was about 1;1, it increased up to 9:1 in heat activated sproes, indicating a subtle change in plasma membrane properties. The permeability of spores increased within 30 min following heat activation as determined by efflux measurements of radioactively labelled spores. At the onset of swelling this efflux was accelerated. During germination the osmotically active material within the spores increased, part of which could be recovered from the supernatant. The combined experiments point to the plasma membrane as possible target site of heat activation in this system.
mesh_terms: D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002463:Cell Membrane Permeability; D004023:Dictyostelium / Q000254:growth & development* / Q000648:ultrastructure; D006358:Hot Temperature; D009235:Myxomycetes / Q000254:growth & development*; D009994:Osmolar Concentration; D013172:Spores, Fungal / Q000254:growth & development / Q000648:ultrastructure