Synthesis of vaccinia viral antigens in HeLa cells in the presence of isatin beta-thiosemicarbazone.
Vaccinia virus antigens, in HeLa cells treated with the antipox virus drug isatin beta-thiosemicarbazone (IBT), were analyzed by immunoprecipitation, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The total radioactivity in the immunoprecipitate was decreased to almost 50% in the presence of the drug as compared to its absence. An inhibition also occurred with an IBT-dependent mutant (IBT(D)) when growing in the absence of IBT. However, similar levels of radioactivity were observed in the immunoprecipitates from an IBT-resistant mutant (IBT(R)) grown in either the absence or presence of the drug. When the antigens within the immunoprecipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it was shown that the inhibition observed in the wild-type-infected cells in the presence of IBT and in the IBT(D)-infected cells in the absence of the drug, was quantitative, affecting the amounts of the different polypeptides more or less equally.
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D007510:Isatin / Q000494:pharmacology*; D009500:Neutralization Tests; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D013882:Thiosemicarbazones / Q000494:pharmacology*; D013997:Time Factors; D014616:Vaccinia virus / Q000276:immunology*
D000956:Antigens, Viral; D007211:Indoles; D013882:Thiosemicarbazones; D007510:Isatin