Modification by capsaicin and compound 48/80 of dye leakage induced by irritants in the rat.
Concentration-related dye leakage produced by intracutaneous injections of irritants was measured in rats by an Evans blue technique. 2 In rats pretreated with a total dose of 50 mg capasaicin over 4 days, the response to capsaicin, formalin, HCl, KCl, prostaglandin E1, bradykinin and bradykinin with prostglandin E1 (10(-6) M) were greatly reduced, the responses to histamine and 5-hydroxytryptamine were slightly reduced and those to adenosine 5'-triphosphate (ATP) and compound 48/80 were unaffected. 3 Pretreatment with intracutaneous injections of compound 48/80 (0.5 mug, 24 and 48 h previously) recuded the responses to ATP, compound 48/80, HCl, KCl, prostaglandin E1, and bradykinin but did not affect those to histamine, 5-hydroxytryptamine or bradykinin with prostaglandin E1 (10(-6) M). 4 Responses to capsaicin and formalin produced spotted blueing extending over a large area and were suppressed by compound 48/80 in the smaller pretreated area only. Capsaicin responses were reduced with larger doses of compound 48/80 (total dose 15 mug). 5 It is concluded that the production of neurogenic oedema involves both sensory nerves and mast cells.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001391:Azo Compounds / Q000378:metabolism*; D001920:Bradykinin / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology*; D004487:Edema / Q000503:physiopathology*; D005070:Evans Blue / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D006851:Hydrochloric Acid / Q000494:pharmacology; D008297:Male; D008954:Models, Biological; D011189:Potassium Chloride / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D051381:Rats; D012701:Serotonin / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D001391:Azo Compounds; D005231:Fatty Acids, Unsaturated; D011458:Prostaglandins E; D005557:Formaldehyde; D012701:Serotonin; D003189:p-Methoxy-N-methylphenethylamine; D005070:Evans Blue; D011189:Potassium Chloride; D006632:Histamine; D000255:Adenosine Triphosphate; D006851:Hydrochloric Acid; D002211:Capsaicin; D001920:Bradykinin