title: Cell-free conversion of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine into an antibiotic with the properties of isopenicillin N in Cephalosporium acremonium.
abstract: Cell-free extracts of antibiotic-negative mutants of Cephalosporium acremonium converted delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (LLD-tripeptide) into an antibiotic that was destroyed by penicillinase. The enzymic activity of the extracts was destroyed by boiling, but was not inhibited by cycloheximide. LLL-Tripeptide was totally inactive as substrate. The product resembled isopenicillin N, but not penicillin N, in its antibacterial spectrum. We propose that isopenicillin N is the first product of cyclization of LLD-tripeptide.
mesh_terms: D000164:Acremonium / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D009842:Oligopeptides / Q000378:metabolism*; D010406:Penicillins / Q000096:biosynthesis* / Q000494:pharmacology
chemical_list: D009842:Oligopeptides; D010406:Penicillins