The noncoordinate expression of muscle-specific proteins in mutant rat skeletal myoblasts and reinitiation of differentiation in hybrids.
In rat skeletal myoblasts which are resistant to 5-azacytidine and fusion-incompetent, the muscle-specific protein, creatine phosphokinase, is produced but muscle-specific myosin is not. In human diploid fibroblast X rat myoblast hybrids, myoblast-specific properties are extinguished. Clones can be selected from the hybrids after a number of doublings which reexpress fusion but do not produce creatine phosphokinase. The conclusion is drawn that the expression of muscle-specific phenotype is not coordinate and fusion of myoblasts is not an essential requirement for the appearance of myoblast-specific proteins.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D003402:Creatine Kinase / Q000096:biosynthesis*; D005809:Genes, Regulator; D006801:Humans; D009124:Muscle Proteins / Q000096:biosynthesis*; D009132:Muscles / Q000166:cytology; D009218:Myosins / Q000096:biosynthesis*; D051381:Rats; D011950:Receptors, Cholinergic / Q000096:biosynthesis
D009124:Muscle Proteins; D011950:Receptors, Cholinergic; D003402:Creatine Kinase; D009218:Myosins