New laboratory scale purification of beta-amanitin from American Amanita phalloides.
A modern laboratory scale method for purifying beta-amanitin from the mushroom Amanita phalloides is described. The purification involves solubilizing the toxin from dried mushrooms, removing the lipids by chloroform extraction, desalting on Amberlite XAD-4, adsorption chromatography on Sephadex LH-20 at acidic and neutral pH, and ion-exchange chromatography on QAE-Sephadex. The resulting beta-amanitin is homogeneous by thin-layer chromatography and is biologically active.
D000363:Agaricales / Q000032:analysis*; D000545:Amanita / Q000032:analysis*; D000546:Amanitins / Q000302:isolation & purification*; D008722:Methods
D000546:Amanitins