Steroid-binding proteins in follicular fluid and peripheral plasma from pigs, cows and sheep.
No unusual steroid-binding proteins that might react with the oocyte or its investments could be detected in follicular fluid. Corticosteroid-binding globulin occurred in follicular fluid from pigs, sheep and cows, and sex hormone-binding globulin occurred in follicular fluid from sheep and cows. The bulk of the steroid in follicular fluid is bound to albumin with low affinity, indicating that steroid molecules can readily be released, and oestrogen can react with the oocyte and granulosa cells in a manner analogous to that demonstrated for target cells bathed with interstitial fluid. Pigs lack a sex-hormone binding globulin in blood plasma and, hence, in follicular fluid. Because no proteins exist in follicular fluid that would compete with antibodies to bind steroids, direct radioimmunoassay of follicular steroids appears to be a valid technique.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000032:analysis; D005260:Female; D006854:Hydrocortisone / Q000032:analysis; D006080:Ovarian Follicle / Q000378:metabolism*; D011374:Progesterone / Q000032:analysis; D011863:Radioimmunoassay; D012738:Sex Hormone-Binding Globulin / Q000032:analysis*; D012756:Sheep; D013552:Swine; D013739:Testosterone / Q000032:analysis; D014156:Transcortin / Q000032:analysis*
D012738:Sex Hormone-Binding Globulin; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D014156:Transcortin; D006854:Hydrocortisone