Identification of vasopressor phospholipid in crude soybean lecithin.
The vasopressor phospholipid in crude soybean lecithin was isolated by column chromatography on Sephadex LH-20. It represented 0.1% of crude soybean lecithin. The isolated phospholipid was identified to be lysophosphatidic acid by gas chromatography-mass spectrometry analysis of TMS-deacylated product and acetolysis product. Nuclear magnetic resonance analysis favored the 1-monoacyl isomer over the 2-isomer. By enzymic determination with L-3-glycerophosphate dehydrogenase, the isolated phospholipid was identified as 1-monoacyl-L-3-glycerophosphate. Gas chromatographic examination revealed that it was composed of a large percentage of unsaturated fatty acids, especially linoleic acid. The activity of isolated lysophosphatidic acid was slightly less than that of synthetic 1-linoleoyl-L-3-glycerophosphate.
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002855:Chromatography, Thin Layer; D013058:Mass Spectrometry; D010713:Phosphatidylcholines / Q000302:isolation & purification*; D010743:Phospholipids / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D013025:Glycine max; D014662:Vasoconstrictor Agents / Q000302:isolation & purification
D010713:Phosphatidylcholines; D010743:Phospholipids; D014662:Vasoconstrictor Agents