The effect of phospholipase C on platelet factor 3 in human blood platelets.
Intact human platelets isolated by gel filtration have been treated with purified phospholipase C. The effect of the enzyme on available and total platelet factor 3 has been tested. The available procoagulant platelet factor 3 was very low. A further small reduction was observed after incubation with phospholipase C when the enzyme was washed away before testing. External attack on platelets by phospholipase C led to a marked inactivation of total platelet factor 3.
D001779:Blood Coagulation Factors; D001792:Blood Platelets / Q000187:drug effects*; D002850:Chromatography, Gel; D006801:Humans; D010740:Phospholipases / Q000494:pharmacology*; D010977:Platelet Factor 3
D001779:Blood Coagulation Factors; D010977:Platelet Factor 3; D010740:Phospholipases