Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction.
We have established 16 independent hybrid lines derived from a fusion between an AKR thymoma and mixed leukocyte reaction-stimulated C57BL/6 spleen cells. The hybrids express both parental H-2 alleles and show hybrid glucose-6-phosphate isomerase activity. Two of the lines showed Fc receptor activity not expressed in the parental thymoma. The AKR Thy-1.1 allele was expressed in all hybrid lines, but no Thy-1.2 could be detected. No killing activity was retained in the hybrid cells, probably because Sendai virus-mediated fusion of killer cells resulted in the lysis of the thymoma fusion partner.
D000818:Animals; D001666:Binding Sites, Antibody; D002459:Cell Fusion; D003602:Cytotoxicity, Immunologic; D004912:Erythrocytes / Q000276:immunology; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D006648:Histocompatibility; D006822:Hybrid Cells; D007141:Immunoglobulin Fc Fragments; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology*; D013945:Thymoma
D007141:Immunoglobulin Fc Fragments; D005956:Glucose-6-Phosphate Isomerase