[Effect of colchicine on polarization of cells on narrow strips of an adherent substrate].
Effect of antitubulin colcemide on polarization of mouse fibroblast-like cells on anisotropic substrate was studied. Such a substrate was obtained by scratching narrow strips in lipid films, adsorbed on the glass. The control cells were seen spread only along the strips, and in 4-6 hours they approached the length of120-150 mcm. In colcemide-containing media, the cells remain in an unspread state for a long time; they extrude their outgrowths both along the strip and perpendicularly to it. Due to frequent refractions of outgrowths, two thirds of colcemide-treated cells were detached from the substrate. Possible mechanisms of these effects of antitubulins are discussed.
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects*; D003078:Colchicine / Q000494:pharmacology*; D051379:Mice
D003078:Colchicine
Vliianie koltsemida na poliarizatsiiu kletok na uzkikh poloskakh adgezivnogo substrata.