Functional state of rat liver RNA polymerase IA and IB.
Phosphocellulose chromatography has been employed to characterize RNA polymerase I present in two different functional states in rat liver cells. The actively transcribing enzyme solubilized from nuclei appears to belong both to the IA and IB classes, whereas the non-transcribing enzyme present in the cytoplasmic fraction has been found to belong only to the IA class. Indirect and direct evidence indicates, however, that in isolated nuclei only the IB form is to be regarded as the physiological form of the enzyme, the IA form arising as a procedural artefact during the extraction process. It may, therefore, be concluded that rat liver IA and IB RNA polymerase are to be strictly regarded as the non-transcribing and transcribing form of the enzyme, respectively.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D003593:Cytoplasm / Q000201:enzymology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D012318:RNA Polymerase I / Q000378:metabolism*; D051381:Rats; D013696:Temperature; D014158:Transcription, Genetic
D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I