Immunological detection of 20beta-hydroxysteroid dehydrogenase-synthesizing polysomes from Streptomyces hydrogenans.
Immunoprecipitation of polyribosomes from Streptomyces hydrogenans synthesizing 20beta-hydroxysteroid dehydrogenase was performed by immunoelectrophoresis. Only polyribosomes from cells induced by 11beta, 21-dihydroxy-4, 11(20)-pregnadien-3-one in vivo were precipitated by immunoglobulins specific for 20beta-hydroxysteroid dehydrogenase. No unspecific precipitations with polysomes from control cells cultivated in the absence of steroids were observed. Therefore, 11beta, 21-dihydroxy-4, 17(20)-pregnadien-3-one increases the number of biological active polyribosomes involved in 20beta-hydroxysteroid dehydrogenase synthesis, presumably by specific stimulation of mRNA synthesis in Streptomyces hydrogenans.
D015089:20-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D003349:Cortisone Reductase / Q000378:metabolism*; D007106:Immune Sera; D007136:Immunoglobulins; D011132:Polyribosomes / Q000201:enzymology*; D012333:RNA, Messenger; D011817:Rabbits / Q000276:immunology; D013302:Streptomyces / Q000201:enzymology / Q000276:immunology*
D007106:Immune Sera; D007136:Immunoglobulins; D012333:RNA, Messenger; D015089:20-Hydroxysteroid Dehydrogenases; D003349:Cortisone Reductase