[Origin of invaginations and vacuoles in liposomes under the effect of endocytosis inducers including oxidants].
The action of various substances on the morphology of multilayer membranes made of lipids of egg yolk was studied. It has been shown that electroneutral and anionic compounds scaresly affected liposomes, whereas substances with pronounced basic properties, i.e. peroxidase, hemoglobin, cytochrome c, and RNAase, as well as lanthanium ions, induced the formation of invaginations, vesicles and aggregation of liposomes. Metals with variable valency: Cu2+, Cu4+, Ru6+, including lipid oxidants Fe3+ and UO+, produced similar morphological changes more intensely and moreover destroyed liposomal membranes. The activator of lipid peroxidation, namely ascorbic acid, intensified while antioxidizers such as alpha-tocopherylacetate and ionol removed the action of Fe3+ on liposomes. A protective effect was displayed by Ca2+ and Mg2+ ions and due to an increase in pH medium. Since many tested substances with the basic properties stimulate endocytosis of cells the processes of lipid peroxidation and electrostatic interactions are supposed to be part of endocytosis mechanism which does not involve the metabolic energy. It is also assumed that endocytosis may arise at the stage of protocells in terms of evolutions.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D004530:Egg Yolk / Q000187:drug effects; D004705:Endocytosis / Q000187:drug effects*; D005260:Female; D008081:Liposomes / Q000032:analysis*; D008563:Membrane Lipids / Q000032:analysis; D009940:Organoids / Q000187:drug effects*; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D014617:Vacuoles / Q000187:drug effects*
D008081:Liposomes; D008563:Membrane Lipids; D010083:Oxidants, Photochemical
Voznikhovenie invaginatsiĭ i vezikul u liposom pod vliianiem induktorov éndotsitoza, v tom chisle oksidantov.