Specific assay for pregnancy-specific-beta 1-glycoprotein (Sp1-beta). Preparation of antiserum specific for SP1-beta by absorption with a crossreacting high molecular weight serum protein.
Two pregnancy-associated proteins reacting with antibody against pregnancy-specific beta 1-glycoprotein (PS beta G or SP1) were separated by means of ammonium sulphate precipitation, size chromatography and preparative zone electrophoresis. The antibody preparation was made monospecific to the beta-mobile protein by liquid phase cross-absorption of anti-SP1 immunoglobulin preparation with the isolated alpha2-mobile high molecular weight protein. The specificity of the absorbed antibody was tested in line immunoelectrophoresis, rocket immunoelectrophoresis and crossed immunoelectrophoresis.
D000042:Absorption; D000918:Antibody Specificity; D001798:Blood Proteins / Q000276:immunology; D011232:Chemical Precipitation; D003429:Cross Reactions; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D007123:Immunoelectrophoresis, Two-Dimensional; D008970:Molecular Weight; D011247:Pregnancy
D001798:Blood Proteins; D006023:Glycoproteins; D007106:Immune Sera