A simple method for elimination of RNAase contamination from DNAase preparations.
RNAase which usually contaminates commercial pancreatic DNAase preparations can be removed by affinity chromatography on agarose-coupled anti-RNAase antibodies. RNA treated with purified DNAase can be re-isolated intact, as determined by polyacrylamide gel electrophoresis under denaturing conditions. This method might be applicable to purification of other preparations which are used in RNA research, such as PNPase (polynucleotide phosphorylase) and specific antibodies for polysome immunoprecipitation. The non-specific binding of DNAase in our system is less than 5% and the loss of specific activity of DNAase I is less than 1%.
D000818:Animals; D002846:Chromatography, Affinity; D003851:Deoxyribonucleases / Q000302:isolation & purification*; D010179:Pancreas / Q000201:enzymology; D012335:RNA, Ribosomal / Q000097:blood; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis; D012260:Ribonucleases / Q000302:isolation & purification*
D012335:RNA, Ribosomal; D003851:Deoxyribonucleases; D012260:Ribonucleases