The vitamin K-dependent synthesis of gamma-carboxyglutamic acid by bone microsomes.
Microsomes prepared from embryonic chick bone contain a vitamin K-dependent carboxylating system which post-translationally converts glutamic acid residues in peptides to gamma-carboxyglutamic acid (gamma-CGlu). Glutamic acid residues in both endogenous chick bone microsomal protein and in the synthetic peptide Phe Leu-Glu-Glu-Val are gamma-carboxylated. These data suggest that bone cells have the capacity for de novo gamma-CGlu synthesis and may be responsible for synthesis of osteocalcin, the major gamma-CGlu protein in bone.
D015055:1-Carboxyglutamic Acid / Q000096:biosynthesis*; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D002642:Chick Embryo; D005971:Glutamates / Q000096:biosynthesis*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009842:Oligopeptides / Q000096:biosynthesis; D014812:Vitamin K / Q000494:pharmacology*; D014859:Warfarin / Q000494:pharmacology
D005971:Glutamates; D009842:Oligopeptides; D014812:Vitamin K; D015055:1-Carboxyglutamic Acid; D014859:Warfarin