title: Euploid somatic recombinants with two active X or XY (1)Y(I) chromosomes isolated from cultured male Indian Muntjac cells after HVJ virus fusion, and their use for gene assignment.
abstract: Four diploid somatic recombinants were isolated from hybrids either between or within two diploid cell lines of a male Indian muntjac after HVJ virus-mediated cell fusion. Both parental lines had a normal male karyotype, 7,X,Y1,Y2, in which the largest autosomal pair was heteromorphic with respect to the size of the secondary constriction (1h+/1h-), C bands, and nucleolar organizers. Of the four recombinants, three showed a 6,XX,1h+/1h+ or 1h+/1h- karyotype, the remaining one a 7,XY1Y2,1h+/1h+. No late-replicating X chromosome was found in the XX recombinants, although it was demonstrated in the natural XX line, suggesting the presence of two active X chromosomes in the former. The G6PD, PGK, and HPRT activities were proportional to the number of active X chromosomes present in all cell types examined, indicating their X-linkage, whereas the same level of activities obtained for LDH and 6PGD indicated their autosomal linkage.
mesh_terms: D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D004261:DNA Replication; D003670:Deer; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D008297:Male; D008938:Mitosis; D010735:Phosphoglycerate Kinase / Q000235:genetics; D011003:Ploidies; D011995:Recombination, Genetic; D012730:Sex Chromosomes / Q000502:physiology*; D014960:X Chromosome / Q000502:physiology*; D014998:Y Chromosome / Q000502:physiology*
chemical_list: D005954:Glucosephosphate Dehydrogenase; D007041:Hypoxanthine Phosphoribosyltransferase; D010735:Phosphoglycerate Kinase