Polarity of actin filaments at the initial stage of myofibril assembly in myogenic cells in vitro.
The polarity of thin filaments in relation to thick filaments in developing muscle cells in vitro was investigated. The majority of thin filaments exhibited the right polarity and spatial position similar to that seen in mature myofibrils. It appears that the interaction between thick and thin filaments exists in the initial phases of myofibrillogenesis. Cortical microfilaments are found to have their polarities arranged randomly.
D000199:Actins / Q000032:analysis*; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D009132:Muscles / Q000166:cytology*; D009210:Myofibrils / Q000648:ultrastructure*
D000199:Actins