Intracellular distribution of virus-specific RNA in chick embryo cells infected with Japanese encephalitis virus.
Japanese encephalitis virus (JEV) infected chick embryo (CE) cells were treated with 4 mug actinomycin D/ml and 5 mM-D-glucosamine at 2 or 3 h before harvesting. Production of JEV was not affected by the short-time treatment of these drugs. The radioactivity in virus-specific RNA in the glucosamine-treated cells was a-parently higher than in non-treated cells. Nuclear and cytoplasmic extracts were prepared from the JEV-infected cells pulse-labelled with 3H-uridine at 15 h after infection. Analysis of virus RNA in nuclear extracts on sucrose density gradients showed that most of the radioactivity was in 23S RNA, 26S RNA and 8 to 12S RNA. The radioactivity of virus RNA in cytoplasmic extracts was found in 42S RNA and RNA fragments sedimenting at less than 8S.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002642:Chick Embryo; D046508:Culture Techniques; D003593:Cytoplasm / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D004664:Encephalitis Virus, Japanese / Q000378:metabolism*; D005944:Glucosamine / Q000494:pharmacology; D012367:RNA, Viral / Q000096:biosynthesis*; D014529:Uridine / Q000378:metabolism
D012367:RNA, Viral; D003609:Dactinomycin; D005944:Glucosamine; D014529:Uridine