[Uptake of L-(+)lactate by cell membrane (luminal and contraluminal) isolated from rat small intestine microvilli].
L-lactate uptake was measured in vesicles formed by intestinal brush border and baso-lateral membranes, using a rapid filtration technique. In the presence of a Na+ gradient directed into the vesicle, L-lactate can be transiently accumulated in brush border vesicles, but not in baso-lateral ones. The transient L-lactate accumulation does not occur in the presence of a KCl gradient. alpha-cyanocinammic acid strongly inhibits L-lactate uptake in brush border vesicles, but not in baso-lateral ones. These results support the existence of a carrier mediated, Na+ dependent, transport of L-lactate across the brush border membrane.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D002934:Cinnamates / Q000494:pharmacology; D007421:Intestine, Small / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism*; D027501:Monocarboxylic Acid Transporters; D009570:Nitriles / Q000494:pharmacology; D009994:Osmolar Concentration; D011189:Potassium Chloride; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D012965:Sodium Chloride
D002934:Cinnamates; D007773:Lactates; D027501:Monocarboxylic Acid Transporters; D009570:Nitriles; D011773:Pyruvates; C018253:alpha-cyanocinnamate; D012965:Sodium Chloride; D011189:Potassium Chloride
Uptake di L-(+)lattato in membrane plasmatiche (luminali e contraluminali) isolate de enterociti di intestino tenue di ratto.