Effect of extracellular K+ concentration on resting potential, caerulein-induced depolarization and amylase release from mouse pancreatic acinar cells.
Acinar cell membrane potentials and amylase release were measured from in vitro preparations of mouse pancreas. The effect of a 10-fold increase of the extracellular K+ concentration (to 47 mM) was studied on the resting membrane potential and amylase release as well as on the membrane depolarization and amylase release induced by the cholecystokinin-pancreozymin analogue, caerulein. In the presence of atropine (to exclude the effect of a possible release of endogenous acetylcholine), the increased K+ concentrations depolarized the cells from -45 to -20 mV without influencing the rate of the unstimulated release of amylase. Under these conditions, the depolarizing effect of caerulein was almost abolished, while the caerulein-induced amylase was not. It is concluded that caerulein-induced enzyme secretion from pancreatic acinar cells is independent of the level of the membrane potential as well as extracellular K+ concentration in the range from 4.7--47 mM.
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002108:Ceruletide / Q000494:pharmacology; D004347:Drug Interactions; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects*; D051379:Mice; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology*
D002108:Ceruletide; D000681:Amylases; D011188:Potassium