[Effect of nerve growth factor and amizil on the viability and metabolism of cultured spinal ganglia].
Spinal ganlia of a 9-day chick embryo were cultivated by the method of "floating rafts" in common medium (control) and in the medium containing amizyl (100 microgram/ml) or a neuregrowth factor (50 microgram/ml). With the action of amizyl there proved to be an increase in the number of surviving neurons; the majority of these neurons contained monoaminoxidase; there was a rise of NAD-diaphorase activity, and, to a lesser extent, of lactic dehydrogenase and isocitric dehydrogenase activities. The neurogrowth factor caused an increase in the number of nerve cells with acetylcholinesterase; there was an elevation of NAD-diaphorase and some rise of malic dehydrogenase activities; the activity of lactic dehydrogenase became maximal; as to succinic dehydrogenase--its activity was somewhat suppressed.
D000818:Animals; D001535:Benactyzine / Q000494:pharmacology*; D002642:Chick Embryo; D002802:Cholinesterases / Q000378:metabolism; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000201:enzymology; D006651:Histocytochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008995:Monoamine Oxidase / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009924:Organ Culture Techniques
D009414:Nerve Growth Factors; D001535:Benactyzine; D007770:L-Lactate Dehydrogenase; D008995:Monoamine Oxidase; D008058:Dihydrolipoamide Dehydrogenase; D002802:Cholinesterases
Vliianie neĭrorostovogo faktora i amizila na perezhivanie i metabolizm kul'tiviruemykh spinal'nykh gangliev.