[Use of N-benzoyl-phenylalanyl-valyl-arginine p-nitroanilide and hirudin for the detection of enzymatic activity under prothrombin acylation by citraconic anhydride].
Acylation of bovine prothrombin by cytraconic anhydride results in the formation of the thrombin active site, which provides the degradation of highly specific thrombin substrate, N-bensoyl-Phe-Val-Arg p-nitroanilide. This reaction is inhibited with hirudin, highly specific thrombin inhibitor.
D000215:Acylation; D000813:Anilides / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002949:Citraconic Anhydrides / Q000494:pharmacology*; D005663:Furans / Q000494:pharmacology*; D006629:Hirudins / Q000494:pharmacology*; D009842:Oligopeptides / Q000378:metabolism*; D011516:Prothrombin / Q000378:metabolism; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000813:Anilides; D002949:Citraconic Anhydrides; D005663:Furans; D006629:Hirudins; D009842:Oligopeptides; D011516:Prothrombin; D013917:Thrombin
Ispol'zovanie N-nitroanilida N-benzonl-fenilalanil-valil-arginina i girudina dlia vyiavleniia fermentativnoĭ aktivnosti pri atsilirovanii protrombina tsitrakonovym angidridom.