Testicular aromatization in immature rats: localization and stimulation after gonadotropin administration in vivo.
Aromatization was measured in testicular microsomal preparations obtained from rats treated 3--4 days with FSH, hCG, or vehicle, hCG, but not FSH, was found consistently to stimulate testicular aromatase activity at least 10-fold. As a marker for FSH action, epididymal androgen-binding protein was assayed and found to be 3 times higher in FSH-treated rats than in either hCG or control rats. hCG, but not FSH or vehicle, stimulated serum testosterone levels more than 100-fold. In all groups, aromatase activity in the microsomal fraction was at least 6 times higher than that found in the mitochondrial fraction. In experiments in which testicular compartments were separated, microsomal preparations from interstitial tissue of hCG-treated rats had 5--7 times more aromatase activity than microsomes from seminiferous tubules and 2--3 times more activity than microsomes from whole testes. It is concluded the hCG administered in vivo can stimulate testicular aromatase activity in immature rats, and the increase in activity is localized in the interstitial tissue.
D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000201:enzymology*; D013739:Testosterone / Q000097:blood
D006063:Chorionic Gonadotropin; D012738:Sex Hormone-Binding Globulin; D013739:Testosterone; D005640:Follicle Stimulating Hormone; D010088:Oxidoreductases; D001141:Aromatase