Tissue culture studies of possible uremic neurotoxins: myoinositol.
Organotypic cultures of dorsal root ganglion cells revealed neuronal toxicity when exposed to myoinositol at doses of 1090 to 109 micrometer (500 to 50 microgram/ml). The ultrastructural changes consisted of membranous cytoplasmic bodies at high concentrations and dense bodies at lower concentrations. There were no changes at 0 to 22 micrometer (0 to 10 microgram/ml). This range of neurotoxicity parallels the levels of myoinositol found in the serum of uremic patients. Myelin loss occurred in a pattern suggesting "secondary demyelination," similar to the pattern seen in uremic neuropathy. The relevance as a possible model for uremic neuropathy is discussed.
D000818:Animals; D046508:Culture Techniques; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure; D003711:Demyelinating Diseases / Q000139:chemically induced; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D007294:Inositol / Q000494:pharmacology*; D051379:Mice; D009498:Neurotoxins; D012583:Schwann Cells / Q000648:ultrastructure
D009498:Neurotoxins; D007294:Inositol