Fructose-induced adenine nucleotide catabolism in isolated rat hepatocytes.
The mechanism of fructose-induced nucleotide catabolism was studied using isolated rat hepatocytes in which the adenine nucleotide pool was prelabelled with [14C]adenine. Incubation of these cells with fructose caused a rapid depletion of the adenine nucleotides and a corresponding increase in allantoin. There was no accumulation of radioactivity in adenosine in the presence or absence of the adenosine deaminase inhibitor 9-erythro-(2-hydroxy-3-nonyl)adenine. This confirms the previous hypothesis that fructose-induced adenine nucleotide catabolism occurs by way of AMP deaminase (AMP amino-hydrolase, EC 3.5.4.6).
D000659:AMP Deaminase / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000378:metabolism*; D000241:Adenosine / Q000378:metabolism; D058892:Adenosine Deaminase Inhibitors; D000481:Allantoin / Q000378:metabolism; D000818:Animals; D005632:Fructose / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D009709:Nucleotide Deaminases / Q000378:metabolism*; D051381:Rats
D000227:Adenine Nucleotides; D058892:Adenosine Deaminase Inhibitors; D005632:Fructose; D000481:Allantoin; D009709:Nucleotide Deaminases; D000659:AMP Deaminase; D000241:Adenosine