Studies on the regeneration of the CFU-C population in blood and bone marrow of lethally irradiated dogs after autologous transfusion of cryopreserved mononuclear blood cells.
In a group of 8 lethally irradiated (1200 R) dogs, that were transfused autologously with cryopreserved mononuclear cells (MNC) derived from the peripheral blood by leucapheresis the concentration of colony-forming units in agar (CFU-C) in bone marrow and peripheral blood was estimated at regular intervals after irradiation and transfusion of MNC. The numbers of MNC transfused per kg body weight ranged from 0.32 x 10(9) to 1.63 x 10(9) with an incidence of CFU-C between 0.02 x 10(5) and 1.38 x 10(5). In 6 dogs the CFU-C levels in the bone marrow reached the normal pre-irradiation values between days 15 and 20. But in 2 dogs that had received the lowest CFU-C numbers the regeneration of the bone marrow CFU-C was markedly delayed. In general the time course of the bone marrow repopulation by CFU-C for single dogs was reflected by a corresponding regeneration pattern of the blood CFU-C. The time course of the curves for the blood CFU-C levels on the other hand was of the same kind as for the granulocyte values in the peripheral blood, thuations were seen in the blood CFU-C levels of single dogs before irradiation and after mononuclear leucocyte transfusion. Despite of such limitations the blood CFU-C content appeared to be a useful indicator of haematopoietic regeneration of the bone marrow.
D000818:Animals; D001772:Blood Cell Count; D001793:Blood Preservation; D001804:Blood Transfusion, Autologous; D001854:Bone Marrow Cells; D002999:Clone Cells; D003470:Culture Media; D004285:Dogs; D005260:Female; D005615:Freezing; D006410:Hematopoiesis / Q000528:radiation effects*; D006412:Hematopoietic Stem Cells; D007962:Leukocytes; D008297:Male; D009000:Monocytes; D011833:Radiation Injuries, Experimental / Q000628:therapy*
D003470:Culture Media