Interaction of coxsackievirus B-3 and peritoneal exudate cells of adult mice treated with cyclophosphamide.
Interaction of coxsackievirus B-3 with peritoneal exudate cells (p.e.c.) of adult mice treated with cyclophosphamide (CP) and of normal untreated mice were examined. The measurement of virus concentration in the supernates and the infective center assay of the virus-treated p.e.c. showed no significant differences in viral uptake between the p.e.c. of mice treated with CP for 1 and 24 hours, and the cells of normal untreated mice. However, a significant decrease in viral uptake was found in p.e.c. of mice collected 72 hours after treatment with CP. This relationship in viral uptake between the CP-treated and normal p.e.c. was reversed when the cells were exposed to antimacrophage serum (AMS). The p.e.c. from normal mice exposed to AMS inactivated significantly less virus than similar cells treated with normal rabbit serum. The peritoneal cell-free fluid of mice treated with CP and coxsackievirus B-3 for 24 hours, contained less virus than the fluid from mice injected with virus alone. It is therefore concluded that the reduction in viral uptake by the p.e.c. derived from mice 72 hours after CP-treatment may contribute to the severity of infection in CP-treated mice.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001202:Ascitic Fluid / Q000166:cytology; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000494:pharmacology*; D004770:Enterovirus / Q000276:immunology*; D007274:Injections, Intraperitoneal; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*
D000961:Antilymphocyte Serum; D003520:Cyclophosphamide