[Enzymatic character and subcellular localization of the transformation reaction of 18 hydroxycorticosterone to aldosterone].
The authors study in vitro the conversion of 18 hydroxycorticosterone into aldosterone in duck adrenal subcellular fractions. The enzymatic nature of this conversion is studied by classical enzymologic tests. All results are in favor of an enzymatic conversion and this enzyme is strictly located in the mitochondrial fraction. Results concerning this localisation are expressed as specific activity and relative specific activity. Preliminary results on further experimental data point out that this conversion depends on the energetic state of mitochondria. Complete electron transfer blokage inhibits this transformation whereas respiratory chain blokage with a NADPH generating system and ATP supplementation does not seem to produce any inhibition. Oligomycine and guanidine stimulation suggest that ATP or its precursors may be involved in this reaction.
D015069:18-Hydroxycorticosterone / Q000378:metabolism*; D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D000450:Aldosterone / Q000096:biosynthesis*; D000818:Animals; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D003345:Corticosterone / Q000031:analogs & derivatives*; D004372:Ducks; D007460:Iodoacetamide / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013696:Temperature
D002729:Chloromercuribenzoates; D000450:Aldosterone; D015069:18-Hydroxycorticosterone; D003300:Copper; D003345:Corticosterone; D007460:Iodoacetamide
Caractère enzymatique et localisaiton subcellulaire de la réaction de transformation de la 18 hydroxycorticostérone en aldostérone.