Studies on human tonsillar lymphocyte membranes. I. Isolation and characterization of a membraneous glycoprotein fraction from human lymphocytes.
A microsomal fraction containing high amounts of plasma membrane has been isolated from human tonsillar lymphocytes. The subcellular fractions were characterized on the basis of their chemical composition and enzyme activities. A glycoprotein fraction solubilized by lithium-diiodo-salicylate (LIS) was purified by ion-exchange chromatography. The purified glycoprotein was analyzed by various methods and its carbohydrate content was determined. The glycoprotein contained large amounts of hexoses and sialic acid and this fraction represented 3--4% of the whole plasma  membrane protein. The fraction was shown to consist of three individual proteins (4 X 10(4), 4.6 X 10(4) and 4.8 X 10(4) daltons) determined by SDS-polyacrylamide gel electrophoresis.
D002241:Carbohydrates / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000648:ultrastructure; D014066:Palatine Tonsil / Q000032:analysis*; D012397:Rosette Formation
D002241:Carbohydrates; D006023:Glycoproteins