In vitro inhibition of human peripheral blood lymphocyte transformation by an extract of Pseudomonas putida.
An extract prepared from a psychrophilic strain of Pseudomonas putida was found to cause a dose-dependent inhibition of [H3]TdR incorporation into human peripheral blood lymphocytes stimulated with PHA, ConA, PWM, or in a mixed lymphocyte reaction. The inhibition was found not to be the result of cytotoxicity, culture medium depletion of a component necessary for lymphocyte transformation, or interference with label uptake by blast lymphocytes. The extract was most effective when added prior to 48 h of mitogen stimulation. The inhibitory material was photeolytic enzyme degradable, heat-stable and non-dialyzable.
D002457:Cell Extracts / Q000494:pharmacology*; D003470:Culture Media; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D011549:Pseudomonas; D013936:Thymidine / Q000378:metabolism; D014020:Tissue Extracts / Q000494:pharmacology*
D002457:Cell Extracts; D003470:Culture Media; D014020:Tissue Extracts; D013936:Thymidine