Purification of a fibrinolysis inhibitor in serum from post-traumatic patients.
A fibrinolysis inhibitor was purified in serum from post-traumatic patients by the use of flat bed electrofocusing of serum desalted by gel chromatography followed by affinity chromatography on a column of matrix-linked plasminogen. Disc gel electrophoresis yielded one protein band. The inhibitor protein was also found in normal serum, but in a lower concentration.
D000933:Antifibrinolytic Agents / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D005337:Fibrin / Q000378:metabolism; D050723:Fractures, Bone / Q000097:blood*; D006801:Humans; D007525:Isoelectric Focusing; D008168:Lung / Q000378:metabolism*
D000933:Antifibrinolytic Agents; D005337:Fibrin