Isolation and partial characterization of normal and defective bacteriophages of gram-negative hydrogen bacteria.
Widespread defective lysogeny was detected in Alcaligenes eutrophus by electron microscopic analysis of cultures. Mitomycin C treatment of the cultures resulted in the production of defective (inco-) particles. Polysheaths were produced both with and without induction. With the simultaneous isolation technique six phages were isolated for hydrogen-oxidizing strains of the new species Pseudomonas pseudoflava. The phages were able to replicate under autotrophic conditions and were found to have a very restricted host range. Electron microscopic analysis allowed classification into two structural groups. Group I contained phages with contractile tails; group II contained phages with flexible, noncontractile tails. All but one (gb) of the new phages were shown to be temperate by isolation of lysogens and induction with mitomycin C.
D000421:Alcaligenes; D001435:Bacteriophages / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D003673:Defective Viruses / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D008242:Lysogeny; D011549:Pseudomonas; D014764:Viral Proteins
D014764:Viral Proteins