title: The origin of the protein synthesis mechanism.
abstract: The origin and development of the protein synthesis mechanism is considered in four successive steps. The genetic code is supposed to be controlled by the relative amount (availability) of various amino acids and nucleotides on the one hand, and utility on each amino acid in the polypeptide. on the other hand. Thus, more simple (inutile) and abundant amino acids tended to correspond to codons which were rich in the less frequent base species, G and C. Features of primitive tRNA in the discrimination of amino acid are discussed. Primitive tRNA is proposed to have a discriminator site for amino acid and, separated from it, an anticodon site for interaction with nucleotides. A hypothetical course of subdivision of various nucleic acid species is proposed. In the scheme, mRNA and ribosomal RNA (rRNA) were derived from more primitive insoluble RNA. DNA appeared in the late, not first, step of the development. Several other aspects of evolutionary development of the whole protein synthesis mechanism, e.g., role of the discriminator site on primitive tRNA, modification and subdivision of code catalogue into a more precise specification of amino acids, and possible primordial interactions between tRNA and tRNA-binding sites on insoluble rRNA, are discussed.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000378:metabolism; D008954:Models, Biological; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D013698:Templates, Genetic; D046249:Transfer RNA Aminoacylation
chemical_list: D003062:Codon; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012313:RNA; D004247:DNA; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases