title: Purification of Bungarus multicinctus antivenin by affinity chromatography.
abstract: Bungarus multicinctus horse antivenin was purified by ammonium sulfate fractionation, gel filtration on Sephadex G-200 column, and affinity chromatography. The specific neutralizing capacity of the antibody preparations showed 2.21-fold, 3.12-fold, and 29.6-fold higher than that of the crude antivenin, respectively.
mesh_terms: D000818:Animals; D000997:Antivenins / Q000302:isolation & purification* / Q000633:toxicity; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D012911:Snakes
chemical_list: D000997:Antivenins