[Effect of temperature on the interaction of cytochrome P-450 with a variety of amines].
The interaction of cytochrome P-450 of rat liver microsomes with six amines have been investigated in Tris-HCL buffer pH 7.4 within the temperature range of 20--37 degrees C by the differential spectrophotometry method. Dissociation constants for the amine-cytochrome-P-450 complexes have been determined. The interaction of type I substrate, 1,2,7-trimethyl-decahydroquinolone-4, is characterized by the value of Ks(I)=4.14 exp (--6250/RT) mole/1. A value of Ks(II)=10(-8) exp (+6500/RT) mole/1 has been obtained for type II substrate, monomethylaniline. Association of 1,2,7-trimethyldecahydroquinolone-4 to cytochrome P-450 decreases with temperature, where as with monomethylaniline the reverse tendency is observed. Thermodynamic parameters delta H, delta F and delta S characterising the interaction of amines with cytochrome P-450 are evaluated.
D000588:Amines; D000814:Aniline Compounds; D000818:Animals; D003577:Cytochrome P-450 Enzyme System; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats; D013696:Temperature; D013816:Thermodynamics
D000588:Amines; D000814:Aniline Compounds; D003577:Cytochrome P-450 Enzyme System
Vliianie temperatury na vzaimodeĭstvie tsitokhroma P-450 nekotorymi aminami.