[A technic for the differentiation of 2 metabolically different cell components, in homogenates or tissue sections, from the distribution of lactate dehydrogenase isoenzymes].
The authors report a mathematic-statistical calculation method for a differentiation analysis of LDH-isoenzyme distribution and the results obtained thereby. The necessity for the development of an improved method of analysis for isoenzymes stems from the fact that even homogenates of very small tissue samples (smaller than 1 mg wet weight) show remarkable statistical inhomogenicity in the distribution of the different fractions. This indicates that cells with very different isoenzyme distribution make up the homogenates and that a cellular inhomogenicity exists and that the origin of isoenzymes is non-uniform, respectively. The previously used description of LDH-isoenzymes is thus insufficient, mis-represented and needs a better differentiation. After representation of the theoretical basis for a differentiating method for analysis of two enzymatically different main cell components (A and B), results from embryonic, adult, degenerated, and malignant tissues are presented and discussed. It is shown that in normal and mature tissues there are always two cell components with different isoenzymes next to each other. It seems that via their metabolites there is a correlation between these apparently metabolically different cell components and they seem to be responsible for the biological homoeostasis over the period of life. Presumably the two cell components produce different glycosaminoglycanes (GAG) and collagen types. In connective tissue certain decoupling mechanisms between the cell components seem to be responsible for the self perpetuation and the chronic course of pathological processes.
D002458:Cell Fractionation / Q000379:methods*; D006651:Histocytochemistry; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008433:Mathematics
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase
Methode zur Differenzierung zweier metabolisch differenter Zellkomponenten in Homogenaten oder Gewebsschnitten aus der Isoenzymverteilung der Lactatdehydrogenase).