[A method for radio-immunological determination of g-strophanthin (author's transl)].
For the purpose of estimating g-strophanthin radio-immunologically the following steps are described: the preparation of g-strophanthin antigen, the production of antibodies in the rabbit, the presentation of calibration curves, and the possibility of a quantitative determination of heterogenous glycosides. According to Malaprades reaction, g-strophanthin adheres to human albumin. With this antigen thus obtained antibodies are produced in rabbits. For the presentation of calibration curves, the addition of antiserum and g-strophanthin have to be matched in quantity; the calibration curves correspond to mathematical data. An increased sensitivity of the method is possible to attain by reducing the amount of labelled antigen and antiserum. The slight cross-reactivity of antibodies can be used to determine other glycosides when these are incubated labelled with tritium instead of 3H-g-strophanthin.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003429:Cross Reactions; D006027:Glycosides / Q000032:analysis; D010042:Ouabain / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay
D006027:Glycosides; D010042:Ouabain
Eine Methode zur radioimmunologischen Bestimmung von g-Strophanthin.