title: Partial perfusion in awake neonatal lambs: a comparison of three methods.
abstract: A comparison of the effects of extended partial perfusion of conscious lambs was made between the use of a silicone-tube membrane oxygenator and a bubble oxygenator (Infant Temptrol). The survival time of the lambs was markedly shortened with the bubble oxygenator, although much longer than had been anticipated. A serious problem was uncontrollable bleeding from the cannulation wounds with a rapidly declining PCV. In a third group of experiments the suitability of Ancrod (Arvin-extract of the venom from the Malayan Pit Viper) as an anticoagulant for extended perfusion with a membrane oxygenator was assessed. In our hands bleeding from the cannulation wounds was marked and difficult to control. The fall in the haematocrit which accompanied the bubble and the arvin series was rapid and appeared to be greater than may have been expected from the loss alone. The decline in several of the plasma components appeared to be due largely to the fall in the haematocrit. Successful perfusion of conscious lambs for up to 96 hours was possible with a silicone-tube membrane oxygenator with Heparin anticoagulation.
mesh_terms: D000721:Ancrod / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001344:Autopsy; D001772:Blood Cell Count; D006400:Hematocrit; D006470:Hemorrhage / Q000473:pathology; D006493:Heparin / Q000494:pharmacology; D007440:Intubation; D008171:Lung Diseases / Q000473:pathology; D010107:Oxygenators, Membrane; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D012756:Sheep; D012826:Silicone Elastomers
chemical_list: D012826:Silicone Elastomers; D006493:Heparin; D000721:Ancrod