Release of alkaline phosphatase from membranes by a phosphatidylinositol-specific phospholipase C.
Purified phosphatidylinositol-specific phospholipase C from Staphylococcus aureus released a substantial proportion of the total alkaline phosphatase activity from a wide range of tissues from several mammalian species. Co-purification of the phospholipase C and alkaline phosphatase-releasing activities and the inhibition of both these activities by iso-osmotic salt solutions suggested that the releasing effect was unlikely to be due to a contaminant.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D006168:Guinea Pigs; D008566:Membranes / Q000187:drug effects / Q000201:enzymology; D010716:Phosphatidylinositols; D010740:Phospholipases / Q000494:pharmacology*; D011817:Rabbits; D013211:Staphylococcus aureus / Q000201:enzymology; D013552:Swine
D010716:Phosphatidylinositols; D010740:Phospholipases; D000469:Alkaline Phosphatase