High-resolution autoradiographic localization of 3-methylcholanthrene-3H in the skin of Balb/c mice after topical application.
Tritiated 3-methylcholanthrene (MC) was traced by electron microscope autoradiography in Balb/c mouse epidermis 24 h after topical application in vivo. Quantification of the autoradiographs indicated that the distribution of radioactivity was non-random. The frequency of silver grains was similar in both nuclei and cytoplasm of epidermal cells. A concentration of labelling was detected in association with nuclear and cell membranes but to a greater degree with the dermo-epidermal junction. Results are consistent with previous work on the binding of hydrocarbons to soluble proteins and nucleic acids. The significance of the association of radioactivity with the dermo-epidermal junction is discussed.
D000287:Administration, Topical; D000818:Animals; D001345:Autoradiography; D008748:Methylcholanthrene / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D012867:Skin / Q000378:metabolism* / Q000648:ultrastructure
D008748:Methylcholanthrene