Effect of thiamine deficiency, pyrithiamine and oxythiamine on pyruvate metabolism in rat liver and brain in vivo.
Rats were fed either a thiamine-deficient diet of diets containing pyrithiamine or oxythiamine. When symptoms of thiamine deficiency appeared, the animals were injected intraperitoneally with [2-14C] pyruvate six to twelve minutes prior to sacrifice. Free glutamic and aspartic acids were isolated from liver and brain and degraded. The results indicate that, in thiamine-deficient or oxythiamine-treated rats, pyruvate metabolism in liver and brain is similar to that in normal animals. In contrast, pyrithiamine drastically decreases the oxidative decarboxylation of pyruvate by rat liver.
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D003653:Decarboxylation; D005971:Glutamates / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D010119:Oxythiamine / Q000494:pharmacology*; D011726:Pyridinium Compounds / Q000494:pharmacology*; D011745:Pyrithiamine / Q000494:pharmacology*; D011773:Pyruvates / Q000378:metabolism*; D051381:Rats; D013832:Thiamine Deficiency / Q000378:metabolism*; D013844:Thiazoles / Q000494:pharmacology*
D005971:Glutamates; D011726:Pyridinium Compounds; D011773:Pyruvates; D013844:Thiazoles; D010119:Oxythiamine; D001224:Aspartic Acid; D011745:Pyrithiamine