[Effect of Ca2+ ions on the pyruvate kinase isoenzymes from rabbit kidney cortex].
Ca2 ions showed the various effect on isoenzymes of pyruvate kinase from rabbit kidney cortex. Ca2 activated the "L" type of pyruvate kinase at low concentrations of PEP and inhibited -- at high concentrations of the latter. "M2" type of pyruvate kinase was inhibited by Ca2 under all the conditions studied. In presence of Ca2+ the activating effect of PDP on "L" and "M2" types of pyruvate kinase was absent; the inhibitory action of ATP on the "M" type of pyruvate kinase was increased at all the concentrations above 1.3 mM. The effect of Ca2+ on the pyruvate kinase isoenzymes depended on content of Mg2+ in the medium.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002413:Cations, Divalent; D004305:Dose-Response Relationship, Drug; D005635:Fructosediphosphates / Q000494:pharmacology; D005943:Gluconeogenesis / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects; D007527:Isoenzymes / Q000378:metabolism*; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008345:Manganese / Q000494:pharmacology; D010728:Phosphoenolpyruvate / Q000494:pharmacology; D011770:Pyruvate Kinase / Q000378:metabolism*; D011817:Rabbits
D002413:Cations, Divalent; D005635:Fructosediphosphates; D007527:Isoenzymes; D008345:Manganese; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D011770:Pyruvate Kinase; D002118:Calcium
Vliianie ionov Ca2+ na izofermenty piruvatkinazy iz kory pochek krolikov.