title: Histidine production by a regulatory mutant of Streptomyces coelicolor.
abstract: Streptomyces coelicolor mutant RF-59, isolated as a revertant of a histidine auxotroph after mutagenic treatment with N-methylN'-nitro-N-nitrosoguanidine, was found to accumulate L-histidine. The mutant was sensitive to 2-thiazo-lealanine and L-2,4-diaminobutyric acid and partially sensitive to alpha-methylhistidine but resistant to 1,2,4-triazolealanine, indicating that repression of the histidine operon was modified in the mutant. Culture conditions were investigated, and optimal media for L-histidine production were developed, resulting in L-histidine accumulation of 2.1 to 3.5 g/liter.
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D003470:Culture Media; D004794:Enzyme Repression; D005809:Genes, Regulator; D006639:Histidine / Q000096:biosynthesis* / Q000494:pharmacology; D008762:Methylhistidines / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine; D009153:Mutagens; D009154:Mutation; D013237:Stereoisomerism; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
chemical_list: D003470:Culture Media; D008762:Methylhistidines; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D006639:Histidine; D000409:Alanine