[Gas liquid chromatographic analysis for quantitative determination of mefruside and oxomefruside in blood plasma (author's transl)].
A specific and sensitive GLC-analytical method was developed for the determination of mefruside, its lactone and hydroxy acid metabolites in blood plasma. It requires the derivatization of the isolated compounds with dimethyl-formamide dimethylacetal and the quantization of the derivatives using a phosphorus nitrogen detector. The method has a sensitivity limit of 2 ng mefruside/ml, 10 ng lactone/ml and 10 ng hydroxy acid/ml, respectively, using 1 ml of blood plasma per assay. It was applied to the determination of plasma levels in a dog following a single oral dose of 10 mg mefruside/kg.
D000818:Animals; D002849:Chromatography, Gas; D004232:Diuretics / Q000097:blood*; D004285:Dogs; D005260:Female; D008529:Mefruside / Q000097:blood*; D008722:Methods; D010084:Oxidation-Reduction
D004232:Diuretics; D008529:Mefruside
Gaschromatographische Analysenmethode zur quantitativen Bestimmung von Mefrusid und Oxomefrusid im Blutplasma.