Kinetic studies of the phenol sulphate-phenol sulphotransferase of Aspergillus oryzae.
Arylsulphatase II of Aspergillus oryzae exhibits both hydrolytic and sulphotransferase activities. The kinetic data suggest the formation of an intermediate covalent enzyme-sulphate complex with transfer of sulphate from donor to acceptor proceeding via a Ping Pong mechanism. The unusual kinetic behaviour when 2-hydroxy-5-nitrophenyl sulphate is the substrate is also consistent with this mechanism.
D001236:Aspergillus oryzae / Q000201:enzymology; D002396:Catechols; D002855:Chromatography, Thin Layer; D006868:Hydrolysis; D007700:Kinetics; D009596:Nitrophenols; D013466:Sulfurtransferases; D014439:Tyramine
D002396:Catechols; D009596:Nitrophenols; D013466:Sulfurtransferases; D014439:Tyramine