Comparison of aldolase isozymes in placenta, HeLa cells, and human fibroblast cultures.
The aldolase specific activity of the human carcinoma cell line, HeLa, against fructose 1,6-diphosphate as substrate is 4- to 5-fold greater than the specific activity of diploid human fibroblast cultures derived from skin and lung. HeLa aldolase is isozyme is predominantly the A type and its substrate preferences resemble human placenta. These findings provide further support for the oncofetal enzyme consitution of HeLa cells.
D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D006367:HeLa Cells / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D012867:Skin / Q000201:enzymology
D007527:Isoenzymes; D005634:Fructose-Bisphosphate Aldolase