title: The assay of influenza virus structural antigens in vaccines by rocket immunoelectrophoresis.
abstract: The internally situated influenza virus structural antigens (matrix and nucleoprotein) and the glycoprotein hemagglutinin were assayed using rocket immunoelectrophoresis or single radial diffusion following disruption of purified virus with 1% w/v sodium sarcosyl detergent. Electrophoresis was carried out for 1-4 h in 0.05 M barbitone buffer at pH 8.6. The potency estimations of HA and M activity were similar for the two methods for bivalent beta-propiolactone inactivated whole virus vaccines.
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D007106:Immune Sera; D007122:Immunoelectrophoresis / Q000379:methods*; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000032:analysis*; D011420:Propiolactone; D012521:Sarcosine / Q000031:analogs & derivatives
chemical_list: D000956:Antigens, Viral; D007106:Immune Sera; D007252:Influenza Vaccines; D011420:Propiolactone; D012521:Sarcosine