Impaired enteric degradation of pancreatic endopeptidases in antibiotic-treated rats.
Conventional Sprague-Dawley rats were fed by gastric tube for 5 days with either benzylpenicillin, ampicillin, doxycycline, or clindamycin. In contrast to the pretreatment period fibrinolytic activity and active and immunoreactive trypsin and immunoreactive elastase were present in fecal extracts after 4 days of antibiotic administration. This is consistent with findings in germfree rats and represents an alteration in the intestinal microflora. The germfree characteristics persisted until a suspension of cecal contents from normal rats was administered by enema on the 26th day. In fecal extracts from the clindamycin-treated rats considerable amounts of active and immunoreactive enzymes were, however, still found 10 days after the enema. A possible explanation is that clindamycin or a metabolite remains in the intestinal tract for a long time. The pathophysiological significance of this finding is unknown.
D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000032:analysis / Q000276:immunology; D002981:Clindamycin / Q000494:pharmacology; D004318:Doxycycline / Q000494:pharmacology; D005243:Feces / Q000201:enzymology; D007422:Intestines / Q000201:enzymology*; D008297:Male; D010196:Pancreatic Elastase / Q000032:analysis / Q000276:immunology; D010400:Penicillin G / Q000494:pharmacology; D051381:Rats; D014357:Trypsin / Q000032:analysis / Q000276:immunology
D000900:Anti-Bacterial Agents; D002981:Clindamycin; D000667:Ampicillin; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin; D004318:Doxycycline; D010400:Penicillin G