Differential in vitro sensitivity of marrow erythroid and granulocytic colony forming cells to chloramphenicol.
The effects of chloramphenicol (CAP) and thiamphenicol (TAP) on mouse and human in vitro erythroid (CFU-E) and granulocytic (CFU-C) colony forming units have been studied. Both drugs inhibited CFU-E growth in a concentration-dependent, stereospecific, manner. Complete inhibition of human CFU-E growth was observed at a CAP concentration of 10 microgram/ml while a concentration ofer 50 microgram/ml was required to inhibit CFU-C growth. Furthermore, whereas the inhibition of CFU-C growth could be blocked in vitro by high colony stimulating factor concentrations, inhibition of CFU-E growth was not affected by increased erythropoietin (ESF) levels. The lack of protection by ESF may account for the apparent vulnerability of erythroid cells to CAP in vivo.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology*; D002999:Clone Cells; D004305:Dose-Response Relationship, Drug; D004900:Erythroblasts / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology*; D006098:Granulocytes / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D013839:Thiamphenicol / Q000494:pharmacology
D004921:Erythropoietin; D002701:Chloramphenicol; D013839:Thiamphenicol