Suppression of the IgE antibody response in mice to kentucky blue grass pollen allergens.
Reagins in murine strains A/HeJ, RF/J C3H/HeJ and B6D2F1 were induced by immunization, in the presence of A1(OH)3, with the retentate (R) obtained by dialysis from the aqueous extract of Kentucky blue grass pollen (KBG-Aq. ext.). Administration of doses of R ranging from 50-200 microgram resulted in IgE antibody responses which were sustained for periods of the order of 6 months. The possibility of suppressing the anti-R IgE antibody response in sensitized A/HeJ mice was tested by the administration into these animals for a period of 14 weeks, biweekly injections of a skin-active, immunogenic fraction (DI) isolated from the dialysate of KBG-Aq. ext. This treatment effectively suppressed the secondary IgE antibody response to R and markedly increased levels of hemagglutinating antibodies. On the basis of the fact that DI comprised only 1.5% of the parent fraction KBG-Aq. ext. and since it was capable of modulating the immune response to R as described, it is suggested that DI may prove to be a useful agent for the immunotherapy of allergies to KBG pollen.
D000536:Aluminum Hydroxide; D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D006386:Hemagglutination Tests; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007165:Immunosuppression Therapy; D007629:Kentucky; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D010323:Passive Cutaneous Anaphylaxis; D010936:Plant Extracts / Q000276:immunology; D006109:Poaceae / Q000276:immunology*; D011058:Pollen; D011852:Radioallergosorbent Test
D010936:Plant Extracts; D007073:Immunoglobulin E; D000536:Aluminum Hydroxide