Preparation of highly purified human somatotropin (growth hormone).
A method for preparation of highly purified human somatotropin on large-scale basis is described. Starting from deep-frozen pituitary gland, less time is needed to obtain highly purified hormone than with other published methods for preparation of human somatotropin. The hormone obtained in this fasion is chromatographically and electrophoretically homogeneous; it shows high biological and radioimmunological growth hormone activity and is free of other pituitary hormone activities. The effects of various experimental conditions upon aggregation of somatotropin are critically evaluated.
D000818:Animals; D001681:Biological Assay; D002356:Cartilage / Q000187:drug effects; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D004305:Dose-Response Relationship, Drug; D013006:Growth Hormone / Q000302:isolation & purification*; D006801:Humans; D010902:Pituitary Gland; D051381:Rats
D013006:Growth Hormone