Allergic drug reactions: an in vitro model using a mixed function oxidase complex to demonstrate antibodies with specificity for a practolol metabolite.
Reactive metabolite products of the drug practolol were generated in vitro using the rat liver mixed function oxidase complex. The metabolites were spontaneously coupled to a non-agglutinating rabbit antibody to human O red blood cells, so forming a metabolite-antibody reagent. This reagent was then used to passively sensitize human O red cells which subsequently acted as indicator cells for detecting anti-metabolite antibodies.
D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004342:Drug Hypersensitivity / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D011217:Practolol / Q000009:adverse effects / Q000276:immunology* / Q000378:metabolism; D051381:Rats
D001323:Autoantibodies; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D011217:Practolol