Alkaline phosphatase phenotypes in tumour and non-tumour cell lines: not an invariable marker for neoplastic transformation.
The cytochemical localisation and presumed isoenzyme type (based on selective inhibition experiments) of alkaline phosphatase in 5 cell lines derived frrom normal human, rat, mouse and hamster tissues, 6 human lymphoblastoid lines and 6 human and mouse tumour-derived cell lines are described. Enzyme activity varied between the cell lines. An isoenzyme inhibited by L-phenylalanine was present in 3 normal lines, 3 lymphoblastoid lines and 2 tumour lines. The presence of this isoenzyme cannot be used as a marker of neoplastic transformation.
D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D002471:Cell Transformation, Neoplastic; D004721:Endoplasmic Reticulum / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D007978:Levamisole / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology; D009685:Nuclear Envelope / Q000201:enzymology; D010649:Phenylalanine / Q000494:pharmacology
D007527:Isoenzymes; D007978:Levamisole; D010649:Phenylalanine; D000469:Alkaline Phosphatase