A photodecomposition fluorimetric method for determination of riboflavin in the various constitutents of blood.
The photodecomposition fluorimetric method has been modified for determination of small quantities of whole blood, erythrocytes, leucocytes, platelets and plasma. The method is accurate in each constituent and contains a test of specificity in each determination. Automation of the method is described. In 13 normal subjects riboflavin in whole blood was range 14.5-24.2, mean 17.7 +/- 2.7 microgram %, in corresponding erythrocytes was range 21.0-44.0, mean 33.7 +/- 6.6 microgram%.
D001792:Blood Platelets / Q000032:analysis; D004912:Erythrocytes / Q000032:analysis; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D007962:Leukocytes / Q000032:analysis; D012256:Riboflavin / Q000097:blood*
D012256:Riboflavin