Purification of mouse interferon on specifically purified immunoadsorbent.
A one-step purification procedure using affinity chromatography on purified anti-interferon antibodies makes possible to obtain highly purified interferon preparations. Antibodies against purified mouse fibroblast interferon were covalently bound to activated CNBr-Sepharose 4B. This immunoadsorbent had a binding capacity of 125,800 mouse fibroblast interferon units per 4.8 ml of gel. The binding capacity for mouse leukocytes was similar while for serum interferon it was lower. When analyzed in polyacrylamide gel, the electrophoretic profiles of purified IF had a similar shape to that of unpurified IF. The results of this purification procedure are not influenced by volume, biological activity and/or purity of the starting material.
D000818:Animals; D000936:Antigen-Antibody Complex; D003488:Cyanogen Bromide; D007164:Immunosorbents; D007372:Interferons / Q000302:isolation & purification*; D007739:L Cells; D051379:Mice; D012685:Sepharose
D000936:Antigen-Antibody Complex; D007164:Immunosorbents; D007372:Interferons; D012685:Sepharose; D003488:Cyanogen Bromide