Synaptosomal plasma membrane glycoproteins. II. Isolation of fucosyl-glycoproteins by affinity chromatography on the Ulex europeus lectin specific for L-fucose.
After solubilization in sodium dodecyl sulphate, almost 90% of synaptosomal plasma membrane glycoproteins were separated from the bulk of synaptosomal plasma membrane proteins by sequential affinity chromatography on two immobilized lectins: concanavalin A and the Ulex europeus lectin specific for L-fucose. Four fractions were obtained and their sugar composition and electrophoretic patterns were determined. Fucosyl-glycoproteins contain more than 26% of protein and 85% of the protein-bound sugar of synaptosomal plasma membrane; hence they constitute a major class of glycoproteins in these membranes. The presence of some glucose in glycoproteins fractions obtained after affinity chromatography on the two lectins suggests that this sugar could be a structural component of some brain glycoproteins. Polyacrylamide gel electrophoresis revealed at least 28 major bands in fucosylglycoprotein fractions, and 11 in other fractions. Several of these major bands appear to contain more than one glycoprotein each. This heterogeneity appears to be mostly the result of the heterogeneity of the neuronal population in the central nervous system. Microheterogeneity of glycoprotein sugar chains and possible contamination of synaptosomal plasma membranes play, in our opinion, only a minor role.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D003208:Concanavalin A; D005260:Female; D005643:Fucose / Q000032:analysis* / Q000276:immunology; D005947:Glucose / Q000032:analysis; D006023:Glycoproteins / Q000302:isolation & purification*; D037102:Lectins; D008297:Male; D051381:Rats; D013570:Synaptic Membranes / Q000032:analysis*
D006023:Glycoproteins; D037102:Lectins; D003208:Concanavalin A; D005643:Fucose; D005947:Glucose