Characterization of lipid A and polysaccharide moieties of the lipopolysaccharides from Vibrio cholerae.
Lipid A and polysaccharide moieties obtained by mild acid hydrolysis of the lipopolysaccharides from Vibrio cholerae 569 B (Inaba) and Vibrio el-tor (Inaba) were characterized. Heterogeneity of lipid A fractions was indicated by t.l.c. and by gel filtration of the de-O-acylated products from mild alkaline methanolysis of the lipids. Presumably lipid A contains a glucosamine backbone, and the fatty acids are probably bound to the hydroxyl and amino groups of glucosamine residues. Approximately equal amounts of fatty acids C16:0, C18:1 and 3-hydroxylauric acid were involved in ester linkages, but 3-hydroxymyristic acid was the only amide-linked fatty acid. Sephadex chromatography of the polysaccharide moiety showed the presence of a high-molecular-weight heptose-free fraction and a low-molecular-weight heptose-containing fraction. Haemagglutination-inhibition assays of these fractions showed the heptose-free fraction to be an O-specific side-chain polysaccharide, whereas the heptose-containing fraction was the core polysaccharide region of the lipopolysaccharides. Identical results were obtained for both organisms.
D002850:Chromatography, Gel; D005227:Fatty Acids / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D008050:Lipid A / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D014734:Vibrio cholerae
D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D005944:Glucosamine