Lecithin/spingomyelin ratio procedure by thin-layer chromatography.
We assessed several modifications of thin-layer chromatography for evaluation of amniotic fluid lecithin/sphingomyelin ratios. For a procedure which is reliable, economical, and easy to perform, we preferred laboratory-prepared plates using SI-LICAR TLC-7GF. For spot detection, we preferred the iodine vapor method.
D000653:Amniotic Fluid / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D006819:Hyaline Membrane Disease / Q000175:diagnosis; D007231:Infant, Newborn; D010713:Phosphatidylcholines / Q000032:analysis*; D012127:Respiratory Distress Syndrome, Newborn / Q000175:diagnosis; D013109:Sphingomyelins / Q000032:analysis*
D010713:Phosphatidylcholines; D013109:Sphingomyelins