Cytotoxicity of commonly used solvents at elevated temperatures.
At 43 degrees C (but not at 41 degrees C), organic solvents used to dissolve water-insoluble chemotherapeutic agents become themselves lethal to cells. This finding is not unique to Chinese hamster cells (HA-1); mouse mammary sarcoma cells (EMT-6) behave similarly. The solvent concentrations involved are in the range of those needed to make drug solutions. Hence experiments measuring drug-cell interactions at elevated temperatures must include controls which independently measure solvent effects.
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004126:Dimethylformamide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D006358:Hot Temperature; D012997:Solvents / Q000494:pharmacology
D012997:Solvents; D000431:Ethanol; D004126:Dimethylformamide; D004121:Dimethyl Sulfoxide