Why HeLa cells do not produce interferon?
The investigation was carried out with HeLa cells which were not sensitive to antiviral action of human diploid cell interferon and do not produce interferon after induction with Newcastle disease virus. Identity of this line was confirmed by determination of stable genetic markers such as isoenzymes and histocompatibility antigens. On electrophoresis of lysed HeLa cells the following human red cell enzymes phenotypes were found: PGM (1-1), AP, EsD, and GTP. It was shown that on the surface of HeLa cells membranes HLA antigens are expressed: A28, BW35, A3. The results are in agreement with those which were obtained from Henrietta Lack's family study. Chromosome G-banding analysis revealed disproportion between the number of chromosomes connected with production and action of interferon and chromosomes which are supposed to control these processes.
D004912:Erythrocytes / Q000201:enzymology / Q000276:immunology; D005260:Female; D006680:HLA Antigens; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D007125:Immunogenetics; D007372:Interferons / Q000096:biosynthesis*; D007621:Karyotyping; D009522:Newcastle disease virus
D006680:HLA Antigens; D007372:Interferons