A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus.
A new restriction endonuclease has been isolated from Bacillus sphaericus R. The purification procedure includes Bio-Gel filtration, (NH4)2SO4 fractionation and phosphocellulose chromatography. After the phosphocellulose step the enzyme preparation is free of non-specific nucleases. Bsp cleaves double-stranded DNA with the same specificity as Bacillus subtilis (Bsu) and Haemophilus aegyptius (HaeIII) restriction endonucleases, as concluded from digests and double-digests of phiX174 replicative form DNA with Bsu and Bsp. The 5'-terminal nucleotide of the cleavage products was shown to be C. Bacillus sphaericus R produces Bsp in extremely large quantities and the enzyme can be easily purified in high yield.
D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000378:metabolism; D013379:Substrate Specificity
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes