Modification of the binding of sulphamidochlorobenzoic acid to human albumin by palmitic acid contamination of albumin.
Palmitic acid a common contaminant of albumin preparations, competitively inhibits the binding of sulphamidochlorobenzoicacid (SCBA) to human albumin thus decreasing its observed affinity. The effect of palmitic acid depends on its concentration, i.e. the purity and concentration of the albumin preparation used. The correct value for SCBA affinity was obtained by correcting the experimental data according to the palmitic acid concentration by use of a multiligand analysis method.
D000418:Albumins / Q000378:metabolism*; D001667:Binding, Competitive; D002723:Chlorobenzoates / Q000378:metabolism*; D004340:Drug Contamination; D006801:Humans; D066298:In Vitro Techniques; D010169:Palmitic Acids / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D013449:Sulfonamides / Q000378:metabolism*
D000418:Albumins; D002723:Chlorobenzoates; D010169:Palmitic Acids; D013449:Sulfonamides