Synthesis of ITP and ATP and regeneration of 2,3-DPG in human erythrocytes incubated with adenosine, pyruvate and inorganic phosphate.
In the stored, 2,3-DPG depleted human erythrocytes incubated for four hours in a medium containing adenosine (10 mM), pyruvate (10 mM), and inorganic phosphate (50 mM) the regeneration of 2,3-DPG reached the value of five times higher than the physiological concentration and the ATP synthesis exceeded four times the physiological level. It has been also found that these erythrocytes are able to synthesize hypoxanthine nucleotides, namely IMP to 58 and ITP to 61 mumoles per 100 ml of erythrocytes.
D000241:Adenosine; D000255:Adenosine Triphosphate / Q000097:blood*; D001793:Blood Preservation / Q000379:methods; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007292:Inosine Nucleotides / Q000097:blood*; D007293:Inosine Triphosphate / Q000097:blood*; D010710:Phosphates; D011773:Pyruvates
D004163:Diphosphoglyceric Acids; D007292:Inosine Nucleotides; D010710:Phosphates; D011773:Pyruvates; D007293:Inosine Triphosphate; D000255:Adenosine Triphosphate; D000241:Adenosine