Ultrastructure of unfixed plasma membranes in ultrathin frozen sections.
Ultrathin sections of unfixed kidney tubular cells were obtained by cryo-ultramicrotomy using the 'wet' method. The results showed that extremely thin sections permit high resolution studies on unfixed plasma membranes. Structural units on the inner surface of the plasma membrane could be detected.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure*; D005629:Frozen Sections; D007687:Kidney Tubules, Proximal / Q000648:ultrastructure; D051379:Mice; D008867:Microtomy