Complementation between mutants of CHO cells resistant to a variety of plant lectins.
Chinese hamster cell mutants resistant to the lectins PHA, WGA, RIC, LCA, and CON A were previously grouped into 8--10 distinct phenotypes on the basis of their unique patterns of lectin resistance and lectin-binding properties. All but one of these classes of lectin-resistant (LecR) mutants behave recessively in somatic cell hybrids. One ricin-resistant class (RicRII) behaves dominantly. Tests for complementation, by measuring the lectin-resistant properties of appropriate hybrids, show that seven distinct complimentation groups can be delineated among the phenotypically recessive mutants.
D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D004351:Drug Resistance; D005796:Genes; D005816:Genetic Complementation Test; D006822:Hybrid Cells; D037102:Lectins / Q000494:pharmacology*; D009154:Mutation; D010641:Phenotype
D037102:Lectins; D003208:Concanavalin A