Effects of Corynebacterium parvum vaccine on drug metabolism in the mouse.
Mice of the CBA strain were given a single intraperitoneal injection of C. parvum vaccine at 50 mg/m2. There followed a progressive increase in liver weight and in the duration of hexobarbitone sleeping time, accompanied by a marked decrease in liver microsomal protein and in cytochrome P-450/mg microsomal protein. That is to say, content of cytochrome P-450 was reduced out of proportion to the apparent loss of endoplasmic reticulum. These changes were paralleled by decreases in microsomal O-demethylation of p-nitroanisole and glucuronide formation with o-aminophenol measured in vitro; they were most marked at 13-14 days after dosing; they then slowly returned to control levels which they reached 20-30 days later. Changes in the dose of vaccine showed that the increase in liver weight and in hexobarbitone sleeping time were significant at 3.5 mg/m2, but that the decrease in the activities measured in vitro were not significant below 35 mg/m2.
D000818:Animals; D001428:Bacterial Vaccines / Q000494:pharmacology*; D004361:Drug Tolerance; D006591:Hexobarbital / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010634:Phenobarbital / Q000378:metabolism; D011425:Propionibacterium acnes / Q000276:immunology*
D001428:Bacterial Vaccines; D006591:Hexobarbital; D010634:Phenobarbital