[Binding of butocin to serum proteins (author's transl)].
The binding of N-[-5-(6-purinylthio)-valeryl]-glycin ethylester (butocin, PVG) to serum proteins and pure human albumin was studied using the method of equilibrium dialysis. Its binding to protein in sera diluted 1:1 of 10 patients with malignant disease averaged 48.4 +/- 7.07%. At the butocin concentration of 20 micrograms/ml an average of 36% of butocin were bound to pure albumin. Only a small portion was bound to globulin fractions. Measurements of the saturation curve showed butocin to be bound to albumin molecule by one binding centre with a microscopic association constant kappa = 1.7 . 10(3) mol/l.
D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D015122:Mercaptopurine / Q000031:analogs & derivatives* / Q000097:blood*; D009369:Neoplasms / Q000097:blood; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism; D012712:Serum Globulins / Q000378:metabolism
D001798:Blood Proteins; D012709:Serum Albumin; D012712:Serum Globulins; D015122:Mercaptopurine
Die Bindung von Butocin an Serumeiweisskörper.