Phytohemagglutinin-induced cytotoxic action of normal lymphocytes on cells in tissue culture using 14C-leucine incorporation.
Stimulation of normal peripheral blood lymphocytes (PBL) by phytohemagglutinin (PHA) and production of PHA-induced cytotoxicity were investigated. PBL transformation stimulated by PHA was measured by 3H-thymidine incorporation, and the cytotoxicity was assayed by measuring the reduction of 14C-leucine incorporation in to target cells after interaction with PHA-treated PBL. The cytotoxicity of PHA-treated PBL showed dose response curves which corresponded with that of PBL transformation. Reduced 14C-leucine incorporation of target cells caused by cytotoxic PBL would be a reliable measure of immune reactions.
D002250:Carbon Radioisotopes; D002470:Cell Survival; D046508:Culture Techniques; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism*; D008213:Lymphocyte Activation
D002250:Carbon Radioisotopes; D037102:Lectins; D004247:DNA; D007930:Leucine