Studies on the action of the nuclear factor promoting actinomycin D-binding capacity of chromatin.
It is well known that actinomycin D binds to C-G pairs of DNA. The amount of actinomycin D bound to chromatin thus depends directly on the demasked sites of chromatin DNA. The actinomycin D binding of rat liver chromatin, obtained by the method of Dingman and Sporn, was studied in the presence and absence of liver and kidney nuclear extracts (NE). The actinomycin D binding of liver chromatin increases greatly under the action of liver nuclear extract. No changes occur in liver chromatin actinomycin D binding capacity after the action of kidney NE. The removal of protein or RNA from liver NE removes its ability to change the actinomycin D binding capacity of the liver chromatin. According to the obtained results it may be assumed that the nuclear extract contains the factor which plays a role in controlling cell differentiation.
D000818:Animals; D001665:Binding Sites; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000502:physiology*; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D003609:Dactinomycin / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000494:pharmacology; D066298:In Vitro Techniques; D007668:Kidney / Q000502:physiology; D008099:Liver / Q000502:physiology; D009928:Organ Specificity; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology
D002843:Chromatin; D003609:Dactinomycin; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases