[Native and desensitized forms of L-type pyruvate kinase from rabbit kidney cortex].
A time-consuming procedure of isolation of pyruvate kinase isoenzymes from rabbit kidney cortex (more than 5 hrs) at 0-2 degrees led to obtaining of a desensitized form of "L" type, resembling the "M3" type of the enzyme from sceletal muscle. Rapid isolation of pyruvate kinase "L" type (within about 2.5 hrs) at 4-6 degrees provided the isoenzyme in the active (allosteric) form.
D000495:Allosteric Site / Q000187:drug effects; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005635:Fructosediphosphates / Q000494:pharmacology; D007527:Isoenzymes / Q000302:isolation & purification*; D007672:Kidney Cortex / Q000201:enzymology*; D008297:Male; D008722:Methods; D011770:Pyruvate Kinase / Q000302:isolation & purification*; D011817:Rabbits; D013696:Temperature; D013997:Time Factors
D000596:Amino Acids; D005635:Fructosediphosphates; D007527:Isoenzymes; D011770:Pyruvate Kinase
Nativnaia i desensibilizirovannaia formy L-tipa piruvatkinazy iz kory pochki krolika.