A simplified colorimetric micromethod for determination of serum cholesterol.
A simple and rapid micromethod is described for quantitation of cholesterol in 5-10 microliter of serum using uranyl acetate to deproteinize the serum and o-phthalaldehyde reagent to develop color. Independent analyses of serum samples by the present method and by two reference methods, Abell-Kendall and automated colorimetric method, indicated accuracy of the micromethod (r = 0.99). Use of capillary blood specimens makes this procedure applicable for screening infants and small children.
D000328:Adult; D002648:Child; D002675:Child, Preschool; D002784:Cholesterol / Q000097:blood*; D003124:Colorimetry / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D008832:Microchemistry; D014501:Uranium
D007202:Indicators and Reagents; D014501:Uranium; D002784:Cholesterol