[Action of cycloheximide on the ultrastructure and metabolic processes in a swine embryo kidney cell culture. I].
Cycloheximide treatment (for 24 hrs, concentrations 1 and 10 microgram/ml) strongly inhibits the intensity of protein and DNA synthesis and the mitotic activity in cells of a pig embryo kidney culture, to a lesser extent inhibits the RNA synthesis in nuclei and nucleoli, reduces the activity of succinate-, lactate- and alpha-glycerophosphate dehydrogenases. There is a condensation of chromatin, a distortion of the granular endoplasmic reticulum integrity, a partial release of its membranes from the ribosomes, changes in the structure of the Golgi complex, morphology and ultrastructure of mitochondria. All these changes are secondary ones and are connected with the suppression of protein synthesis in cells.
D000818:Animals; D001345:Autoradiography; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects*; D003513:Cycloheximide / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000378:metabolism / Q000648:ultrastructure; D007930:Leucine / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D013552:Swine; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014529:Uridine / Q000378:metabolism
D012313:RNA; D004247:DNA; D003513:Cycloheximide; D007930:Leucine; D013936:Thymidine; D014529:Uridine
Deĭstvie tsiklogeksimida na ul'trastrukturu i nekotorye metabolicheskie protsessy kletok kul'tury SPEV. Soobshchenie I.