title: Liquid nitrogen storage of yeast cultures. I. Survival, and literature review of the perservation of fungi at ultralow temperatures.
abstract: All 20 yeast strains of 17 species tested survived 75 days (the length of the experimental period) in liquid nitrogen at -196 C. The components of the more protective of the two freezing media used were (w/v) malt extract 2.5%, yeast extract 0.25%, peptone 0.5%, calf serum 15% (v/v) and dimethyl sulfoxide 10% (v/v). Viability of the cells in this medium after rapid uncontrolled freezing and thawing in selaed plastic ampoules ranged from 2% to 98% (average 67%) compared with the viability of the cultures before freezing. In only 4 strains was survival lower than 50%. (90 references).
mesh_terms: D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005615:Freezing; D011309:Preservation, Biological; D013045:Species Specificity; D015003:Yeasts / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*
chemical_list: D003470:Culture Media; D004121:Dimethyl Sulfoxide