Chemical synthesis of two deoxyribododecanucleotides for the attachment of restriction termini to an artificial minigene.
In order to permit in vivo cloning of an artificial minigene designed to code for a modified S-peptide, the phosphodiester method for the chemical synthesis of two dodecadeoxyribonucleotides is described. Each of the latter possesses antiparallel complementarity to one of the two minigene strands and to the single-stranded EcoRI-generated end. They can thus serve as cohesive termini ("splints") for polynucleotide ligase joining.
D001483:Base Sequence; D004274:DNA, Recombinant; D003854:Deoxyribonucleotides / Q000138:chemical synthesis*; D005815:Genetic Code; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011118:Polynucleotide Ligases / Q000378:metabolism; D012313:RNA / Q000235:genetics
D004274:DNA, Recombinant; D003854:Deoxyribonucleotides; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D012313:RNA; D011118:Polynucleotide Ligases