Nuclear labelling of leukaemic blast cells with tritiated thymidine triphosphate in 35 patients with acut leukaemia.
A new technique which detects the presence of DNA polymerase and primer-template DNA by measuring the in vitro incorporation of [3H]thymidine-5-triphosphate (3H-TTP) into nuclei of leukaemic blast cells (LBC) was used in 35 patients with acute leukaemia. The 3H-TTP labelling index (3H-TTP LI) exceeded the fraction in DNA synthesis by a factor 1.4-24.3. The values of 3H-TTP labelling in the bone marrow always exceeded those obtained in the blood. In addition 10 normal bone marrows were studied; here, the 3H-TTP LI either exceeded or equalled the fraction of the proliferative pool in DNA synthesis.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D002467:Cell Nucleus / Q000378:metabolism; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D007938:Leukemia / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation; D008297:Male; D008722:Methods; D008875:Middle Aged; D008938:Mitosis; D013942:Thymine Nucleotides / Q000378:metabolism
D004273:DNA, Neoplasm; D013942:Thymine Nucleotides; D004259:DNA-Directed DNA Polymerase