Fertilization and preimplantation development in vitro of mouse eggs obtained following stimulation with different doses of pregnant mare serum: a comparison of the responses in two strains.
Unfertilized eggs were recovered from TO and (C57BL/10 X CBA)F1 females, induced to ovulate with 0.75--10.0 IU PMS and 5.0 IU HCG/mouse, and mixed with TO sperm in vitro. Among the groups of F1 eggs, there were no significant differences in either fertilization or development to the blastocyst stage in vitro. With TO eggs, however, there was significantly lower fertility in the 0.74 and 1.5 IU PMS groups than in the higher PMS dose groups. Conversely, preimplantation development was significantly better withe low doses. To determine whether polyploidy migh affect viability in vitro, digynic polyploidy was experimentally induced in both F1 and TO eggs with cytochalasin B. Development of polyploid F1 X TO embryos to the blastocyst stage did not differ significantly form that of untreated embryos. Development of polyploid TO X TO embryos obtained from eggs stimulated with 1.5 IU PMS was still higher than that for eggs stimulated with 7.5 IU PMS. This suggests that cytoplasmic inadequacies, rather than genome imbalance, are responsible for the reduced ability of the eggs stimulated with high doses of PMS to reach the blastocyst stage in vitro.
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010060:Ovulation / Q000187:drug effects; D011123:Polyploidy; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D013045:Species Specificity
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D003571:Cytochalasin B