Carbohydrate composition of purified human liver alpha-L-fucosidase.
Human liver alpha-L-fucosidase was purified to apparent homogeneity and analyzed for carbohydrate content primarily by gas-liquid chromatography (glc). The enzyme is about 7% carbohydrate by weight and contains the following sugars (residues per 50, 000 molecular weight subunit): mannose (8.3), glucosamine (4.3) (presumably N-acetylated), sialic acid (1.6) and glucose (1.6). Galactose (0.8) and L-fucose (1.8) were also found but their presence may be due to artifacts of the purification procedure.
D002241:Carbohydrates / Q000032:analysis*; D005944:Glucosamine / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006801:Humans; D008099:Liver / Q000201:enzymology*; D012794:Sialic Acids / Q000032:analysis; D005644:alpha-L-Fucosidase / Q000032:analysis*
D002241:Carbohydrates; D006601:Hexoses; D012794:Sialic Acids; D005644:alpha-L-Fucosidase; D005944:Glucosamine