title: The peptidoglycan crosslinking enzyme system in Streptomyces strains R61, K15 and rimosus.
abstract: The DD-carboxypeptidase-transpeptidase enzyme system in Streptomyces strain K15 consists of: (1) a membrane-bound transpeptidase capable of performing low DD-carboxypeptidase activity; and (2) a set of DD-carboxypeptidases: (a) membrane-bound, (b) lysozyme-releasable and (c) exocellular, having low transpeptidase activities in aqueous media and at low acceptor concentrations. The DD-carboxypeptidases are related to each other and may belong to the same pathway leading to enzyme excretion. A similar enzyme system occurs in Streptomyces strain R61 except that the membrane-bound DD-carboxypeptidase activity is low when compared with the membrane-bound transpeptidase activity. In Streptomyces rimosus the enzyme system consists almost exclusively of the membrane-bound transpeptidase and the levels of membrane-bound, lysozyme-releasable and exocellular DD-carboxypeptidases are very low.
mesh_terms: D002268:Carboxypeptidases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000302:isolation & purification / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism*; D013045:Species Specificity; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D010457:Peptidoglycan; D002268:Carboxypeptidases; D002267:Muramoylpentapeptide Carboxypeptidase