Effect of non-fat dry milk on recovery of staphylococcal thermonuclease from foods.
Modification of the method of Tatini et al. (1976) by addition of non-fat dry milk (NFDM) to food samples and subsequent acid precipitation at pH 3.8 enhanced the recovery of staphylococcal thermonuclease (TNase) from most of 37 foods tested. The modified TNase assay method allowed detection of 10 ng (0.002 units) of the enzyme per gram of each of the following foods: ground beef, boiled egg products, whey powder, fruit-containing yogurt, dressings and spreads, potato and egg salads, and pastas, all of which gave false-negative results without NFDM.
D000818:Animals; D005506:Food Contamination; D005516:Food Microbiology; D006358:Hot Temperature; D008722:Methods; D008836:Micrococcal Nuclease / Q000032:analysis* / Q000302:isolation & purification; D008892:Milk; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000254:growth & development
D008836:Micrococcal Nuclease