Horizontal polyacrylamide gradient gel electrophoresis for the simultaneous phenotyping of transferrin, post-transferrin, albumin and post-albumin in the blood plasma of cattle.
A simple method of horizontal polyacrylamide gel electrophoresis was described for the simultaneous phenotyping of transferrin, post-transferrin, albumin and post-albumin in the blood plasma of cattle. A step gradient gel of 8, 4, 12 and 14% acrylamide concentration was used. The method enabled the detection of a new protein polymorphism in the post-transferrin region. Two alleles were observed. The transferrin phenotypes involving D1 and D2 alleles were clearly separated. The resolution of the post-albumin fractions was also better than described by earlier methods.
D000483:Alleles; D000818:Animals; D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000235:genetics; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D010641:Phenotype; D011110:Polymorphism, Genetic; D012710:Serum Albumin, Bovine / Q000235:genetics*; D014168:Transferrin / Q000235:genetics*
D001798:Blood Proteins; D014168:Transferrin; D012710:Serum Albumin, Bovine