Production of [14C]rubratoxin B.
[14C]rubratoxin B was produced by culturing Penicillium rubrum Stoll for 13 days at 22 degrees C in medium containing [14C]glucose. The most efficient incorporation of glucose into rubratoxin occurred when Raulin-Thom medium enriched with 2.5% malt extract was supplemented with 2.5% added glucose. The presence of 1.0 mCi of radioactivity in 50 ml of medium with 2.5% added glucose resulted in the production of 38 mg of labeled, chromatographically pure rubratoxin with a specific activity of 0.47 Ci/mole.
D000818:Animals; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D008168:Lung / Q000382:microbiology*; D009183:Mycotoxins / Q000096:biosynthesis*; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism*; D014422:Turkeys / Q000382:microbiology*
D003470:Culture Media; D009183:Mycotoxins; D005947:Glucose