Purification of commercial acriflavine by Sephadex LH-20 column chromatography.
Acriflavine supplies commercially was purified by removing contaminating proflavine by chromatography on a column of Sephadex LH-20 eluded with 65% methanol. The purified acriflavine, recovered in a yield that was 52% of the theoretical maximum, had a visible absorption maximum of 452 nm with a band width of 47 nm. These spectral properties agreed with those of acriflavine purified by precipitation, repeated recrystallization and chromatography on aluminum oxide, which results in a yield of 13% at best.
D000166:Acridines / Q000302:isolation & purification*; D000167:Acriflavine / Q000302:isolation & purification*; D002850:Chromatography, Gel / Q000379:methods; D013053:Spectrophotometry
D000166:Acridines; D000167:Acriflavine