The effect of the DNA-suppressing factor (DSF) on host DNA synthesis in synchronized cell cultures.
Purified host DNA-suppressing factor (DSF) produced into culture fluid of HeLa C-9 cells infected with measles virus inhibited cellular DNA synthesis in HeLa cells. When purified DSF was added into cultures of synchronous HeLa cells at the early G1-phase, cellular DNA synthesis was irreversibly inhibited. However, DSF did not affect the stability of native double-stranded DNA nor the chain-elongation of single-stranded DNA in cells of the S-phase.
D002478:Cells, Cultured; D004279:DNA, Viral / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D006367:HeLa Cells / Q000378:metabolism; D008459:Measles virus / Q000378:metabolism; D013997:Time Factors
D004279:DNA, Viral