[Indirect method of determining drug-metabolizing enzyme activity and its clinical application].
Activity of microsomal enzymes was studied using an indirect method for estimation of the rate of antipyrine secretion with saliva in 70 healthy persons at the age from 16 to 86 years. Distinct individual differences were observed in the activity of the enzymes: time of the antipyrine half-secretion varied from 6.4 hrs to 50.1 hrs. The time of antipyrine half-secretion was significantly higher in the older (60-86 years) than in younger persons (16-30 years), suggesting decreased activity of the microsomal enzymes in older people. The microsomal enzymes activity might be increased distinctly under effect of several drugs, especially of hypotensive drugs--reserpine and dibasole. The data obtained suggest that for effective medicinal therapy it is essential to control the initial activity of microsomal enzymes as well as its alterations in the course of medicamentous treatment; the activity of microsomal enzymes in a given person must be taken into consideration for correct individual selection of doses of the drugs.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000983:Antipyrine / Q000032:analysis; D001562:Benzimidazoles / Q000494:pharmacology; D001711:Biotransformation; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D008861:Microsomes / Q000201:enzymology*; D008875:Middle Aged; D010276:Parasympatholytics / Q000494:pharmacology; D012110:Reserpine / Q000494:pharmacology; D012463:Saliva / Q000032:analysis
D001562:Benzimidazoles; D010276:Parasympatholytics; D012110:Reserpine; D000983:Antipyrine
Nepriamoĭ metod opredeleniia aktivnosti fermentov, metaboliziruiushchikh lekarstvennye veshchestva, i ego primenenie v klinike.