Specificity of the cholesterol side-chain cleavage enzyme system of adrenal cortex.
Incubation of lanosta-8, 24-dien-3beta-o1-1,2-3H and lanost-8-en-3beta-o1-1,2-3H with an adrenocortical bovine mitochondrial acetone-dried preparation did not yield any significant (less than 0.01%) 3beta-hydroxy-4, 4, 14-trimethyl-5alpha-pregn-8-en-20-one. Under the same conditions cholesterol-1,2-3H yielded 8.3% pregnenolone. Incubation of (20S)-17alpha, 20-di-hydroxycholesterol-7-3H yielded 0.6 to 1.6% (20S,22R)-17alpha, 20, 22-trihydroxycholesterol, 1.0 to 3.2% of 17alpha-hydroxy-pregnenolone, but no significant (less than 0.02%) (20S,22S)-17alpha,20,22-trihydroxycholesterol. In another experiment incubation of cholesterol-1,2-3H yielded 5% pregnenolone, 0.5% 17alpha-hydroxypregnenolone, 0.2% (20R,22R)-20,22-dihydroxy-cholesterol, but no significant ( less than 0.01%) 17alpha-hydroxy-cholesterol, (20S)-17alpha, 20-dihydroxycholesterol or (20S,22R)-17alpha, 20,22-trihydroxycholesterol.
D000302:Adrenal Cortex / Q000201:enzymology* / Q000648:ultrastructure; D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000031:analogs & derivatives / Q000378:metabolism; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism*; D066298:In Vitro Techniques; D007810:Lanosterol / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism*; D011284:Pregnenolone / Q000031:analogs & derivatives / Q000096:biosynthesis; D013379:Substrate Specificity
D007810:Lanosterol; D011284:Pregnenolone; D002784:Cholesterol; D010088:Oxidoreductases; D002786:Cholesterol Side-Chain Cleavage Enzyme