A reference system in crossed radioimmunoelectrophoresis.
A reference system for crossed radioimmunoelectrophoresis (CRIE) has been developed. The system takes into account the variable quality of the 125I-labeled anti-IgE and permits a direct comparison of the results from one experiment to another. Simultaneously with the preparation of the CRIE slides, a standard series of small paper discs are prepared containing varying, defined amounts of 125I-labeled anti-IgE. These paper discs are laid out for autoradiography together with the CRIE slides. After short periods of exposure, only paper discs with large amounts of isotope are visible on the X-ray films. Longer exposure times permit paper discs with smaller amounts of isotope to be visible. Each precipitate is assigned to a Crie class depending on the number of paper discs visible when the precipitate is detected for the first time.
D000941:Antigens; D006801:Humans; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007073:Immunoglobulin E; D011852:Radioallergosorbent Test; D011863:Radioimmunoassay
D000941:Antigens; D007106:Immune Sera; D007073:Immunoglobulin E