Isolation of alpha-glucan and lipopolysaccharide fractions from Acetobacter xylinum.
A cellular phenol-water extract of Acetobacter xylinum NRC 17007 was fractionated on Sepharose 4 B. The fraction eluting with the void volume consisted to about 95% of glycogen-like material. The lipopolysaccharide fraction was of lower molecular weight and had the following composition (%, w/w): Mannose, 42; glucose, 7; galactose, 3.8; heptose, 2; 2-keto-3-deoxy-octonate, 1.2; glucosamine, 3.3; phosphate, 4.5; total fatty acids, 3.9. Among the fatty acids, 3-hydroxy-tetradecanoic acid was present, and 2-hydroxy-hexadecanoic acid predominated.
D002241:Carbohydrates / Q000032:analysis; D002847:Chromatography, Agarose; D005227:Fatty Acids / Q000032:analysis; D041822:Gluconacetobacter xylinus / Q000032:analysis*; D006539:Heptoses / Q000032:analysis; D007661:Ketoses; D008070:Lipopolysaccharides / Q000032:analysis / Q000302:isolation & purification*; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D013400:Sugar Acids / Q000032:analysis
D002241:Carbohydrates; D005227:Fatty Acids; D006539:Heptoses; D007661:Ketoses; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids