3H-Spiroperidol labels dopamine receptors in pituitary and brain.
3H-Spiroperidol of high specific radioactivity labels dopamine receptors in membranes of bovine caudate nucleus and anterior pituitary. The saturation and kinetic properties of 3H-spiroperidol binding are similar in the two tissues. In both caudate and pituitary 3H-spiroperidol displays very high affinity with a dissociation constant of 0.2-0.3 nM. The relative potencies of numerous dopamine agonists and antagonists in competing for 3H-spiroperidol binding are closely similar in anterior pituitary and caudate.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002090:Butyrophenones; D002417:Cattle; D003864:Depression, Chemical; D066298:In Vitro Techniques; D010902:Pituitary Gland / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000378:metabolism*; D013134:Spiperone / Q000378:metabolism
D002090:Butyrophenones; D011954:Receptors, Dopamine; D013134:Spiperone