Transcriptional reactivation of isolated Xenopus erythrocyte nuclei: patterns of RNA synthesis.
Nuclei isolated from Xenopus erythrocytes can be transcriptionally reactivated by exposure to certain cytoplasmic proteins. The types of RNA synthesized during this reactivation have been studied and compared with those present in, or synthesized by, isolated nuclei not so reactivated or in entire Xenopus erythrocytes. In all cases, the pattern of transcription indicates the synthesis of a broad range of low molecular weight RNAs. Competitive hybridization demonstrates that the reactivated nuclei synthesize some transcripts not normally produced by the isolated nuclei and we have shown that a proportion of these possess amino acid-accepting activity. The significance of these results is discussed in relation to the control of gene activity in these cells.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D066298:In Vitro Techniques; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000096:biosynthesis; D014158:Transcription, Genetic; D014981:Xenopus / Q000097:blood
D000596:Amino Acids; D012313:RNA; D012343:RNA, Transfer