Lymphocyte stimulation in multiple sclerosis patients untreated and treated with transfer factor. Long-term studies on the effect of different stimulants on myo-(2-3H)inositol incorporation into phosphatidylinositol of lymphocytes.
The lymphocytes from controls, untreated MS and MS patients treated with transfer factor were stimulated with PHA, PWM, PPD, LPS and MA and the increased incorporation of myoinositol into phosphatidylinositides was determined. As compared with controls, the untreated MS patients revealed significantly lower index of stimulation with PHA, LPS, PPD and MA. The response of lymphocytes from MS patients treated with transfer factor was intermediate. No correlation between disease progression and stimulation of inositol incorporation was observed. Analysis of variance showed that the myoinositol test is not applicable in long-term studies to distinguished single subjects, however it is useful to determine differences between MS patients and controls.
D000328:Adult; D000956:Antigens, Viral / Q000008:administration & dosage; D005260:Female; D006801:Humans; D007294:Inositol / Q000378:metabolism*; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008459:Measles virus / Q000276:immunology; D008934:Mitogens / Q000494:pharmacology; D009103:Multiple Sclerosis / Q000276:immunology* / Q000628:therapy; D010716:Phosphatidylinositols / Q000378:metabolism*; D013997:Time Factors; D014165:Transfer Factor / Q000627:therapeutic use*; D014373:Tuberculin / Q000494:pharmacology
D000956:Antigens, Viral; D037102:Lectins; D008070:Lipopolysaccharides; D008934:Mitogens; D010716:Phosphatidylinositols; D014165:Transfer Factor; D014373:Tuberculin; D007294:Inositol