Purification of C-reactive protein on DEAE-cellulose by a simple two-step procedure utilizing the calcium-dependency of the protein.
A simple procedure for isolating C-reactive protein is presented. The method consists of two chromatographic separations on diethylaminoethyl (DEAE)-cellulose columns and utilized the difference between the binding of C-reactive protein to DEAE in the presence and absence of calcium. Electrophoretically pure C-reactive protein can be recovered from serum or ascitic fluid with a yield of approx. 50--60% within one day.
D002097:C-Reactive Protein / Q000302:isolation & purification*; D002118:Calcium; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D006801:Humans; D007118:Immunoassay; D007122:Immunoelectrophoresis; D011485:Protein Binding
D002097:C-Reactive Protein; D002118:Calcium