Quantitation of radio-labelled vitamin K1 and vitamin K1 epoxide in plasma by high pressure liquid chromatography.
A convenient, accurate and reproducible high pressure liquid chromatographic method for the quantitation of radio-labelled vitamin K1 and vitamin K1 epoxide in plasma is described. The method involves the determination of total ether extractable radioactivity, and a chromatographic separation to determin the relative quantities of radio-labelled vitamin K1 and vitamin K1 epoxide. The method is useful over a wide range of ratios of the two compounds, and has a coeffcient of variation of approximately 5%.
D002851:Chromatography, High Pressure Liquid; D004852:Epoxy Compounds / Q000032:analysis*; D004988:Ethers, Cyclic / Q000032:analysis*; D006801:Humans; D008297:Male; D008722:Methods; D014812:Vitamin K / Q000032:analysis* / Q000097:blood; D014859:Warfarin / Q000627:therapeutic use
D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D014812:Vitamin K; D014859:Warfarin