Production of anti-rubratoxin antibody and its use in a radioimmunoassay for rubratoxin B.
Rubratoxin B was coupled to ovalbumin using 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide HCl (ECDI) in high concentration at pH 8.0. Under these conditions it was possible to couple 13 moles of rubratoxin B per mole of ovalbumin. The conjugate was used for immunization of rabbits, and anti-rubratoxin antibody was produced. A radioimmunoassay for rubratoxin B was developed which could detect 0.1 microgram to 10 microgram of toxin using 0.21 microgram of [14C] rubratoxin (0.47 Ci/mole, 2.0 x 10(9) dpm/microgram) and 0.125 ml of anti-rubratoxin antibody.
D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D009183:Mycotoxins / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*
D000906:Antibodies; D009183:Mycotoxins