title: A new well-characterized, purified allergen preparation from timothy pollen. I. Chemical properties.
abstract: A purified allergen preparation, Timothy N, from timothy pollen (Phleum pratense) was characterized with respect to chemical properties in comparison with a commercially available crude extract, Timothy O, from the same pollen material. In Timothy N about 94% of the protein and 99% of both hexoses and pentoses were removed during the purification. Timothy N contains a limited number of proteins with molecular weights of about 15,000 and 30,000. A Timothy N solution of 0.010 mg/ml will have an allergenic activity of 1 HEP.
mesh_terms: D000485:Allergens / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006632:Histamine / Q000378:metabolism; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010940:Plant Proteins / Q000032:analysis; D011058:Pollen; D012882:Skin Tests; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000485:Allergens; D010940:Plant Proteins; D006632:Histamine