title: The involvement of the bridging imidazolate in the catalytic mechanism of action of bovine superoxide dismutase.
abstract: The pulse-radiolysis method has been used to study the catalytic mechanism of O2 leads to dismutation by the Co(II)-substituted bovine erythrocuprein (superoxide dismutase, EC 1.15.1.1). Catalysis is accompanied by spectral changes that may be interpreted in terms of rapid protonation and deprotonation of the Cu-facing nitrogen atom of the imidazolate that bridges the Cu(II) and the Co(II) [or Zn(II)] in the oxidized enzyme. This rapid change permits the possibility that the imidazole is a proton donor in the catalytic reduction of O2 leads to.
mesh_terms: D000818:Animals; D002417:Cattle; D007093:Imidazoles; D007700:Kinetics; D011522:Protons; D011675:Pulse Radiolysis; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D007093:Imidazoles; D011522:Protons; D013482:Superoxide Dismutase