title: Acid releases motilin from human duodenum in vitro.
abstract: The effect of hydrochloric acid and sodium hydroxide on motilin release from human duodenal mucosa was studied in vitro. Tissue was incubated in 2 ml chambers continuously perfused with medium and was exposed repeatedly to the stimuli. Motilin released into the medium was determined by a specific radioimmunoassay. Whereas alkalinisation abolished basal motilin release, acidification resulted in a 14-fold increase of peptide secretion. The results indicate that motilin secretion can be stimulated in vitro and that the perfusion system described may be a useful tool in studying the mechanism of gastrointestinal hormone release.
mesh_terms: D004386:Duodenum / Q000187:drug effects / Q000502:physiology; D005768:Gastrointestinal Hormones / Q000378:metabolism*; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000502:physiology; D009037:Motilin / Q000378:metabolism*; D012972:Sodium Hydroxide / Q000494:pharmacology*
chemical_list: D005768:Gastrointestinal Hormones; D009037:Motilin; D012972:Sodium Hydroxide; D006851:Hydrochloric Acid