Studies on a rat liver subcellular poly A-rich RNA fraction with glutamate dehydrogenase template activity.
A heterogeneous poly A-mRNA fraction was isolated from rat liver microsomes by phenol:chloroform extraction, millipore filtration, and poly U-agarose affinity chromatography. The fractions were characterized by their secondary structures and poly A contents. From translational studies, the isolated fraction was found to have high glutamate dehydrogenase template activity in cell-free systems containing microsomes or polysomes. A spectrophotometric procedure for following enzyme biosynthesis was also developed.
D000818:Animals; D002846:Chromatography, Affinity; D005969:Glutamate Dehydrogenase / Q000096:biosynthesis; D008862:Microsomes, Liver / Q000032:analysis*; D011061:Poly A / Q000302:isolation & purification*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification*; D051381:Rats; D013698:Templates, Genetic
D012333:RNA, Messenger; D011061:Poly A; D005969:Glutamate Dehydrogenase