title: Physical properties of Tamm-Horsfall glycoprotein and its glycopolypeptide.
abstract: The molecular weight of the constituent glycopolypeptide chain of T-H glycoprotein was determined by sedimentation equilibrium under two entirely different sets of denaturing conditions. For both sets of denaturing conditions, the average molecular weight estimated for T-H glycopolypeptide was 74,000. The gel chromatographic behavior in 6M guanidium chloride of T-H glycopolypeptide with disulfide bonds intact as compared with its gel chromatographic behavior with disulfide bonds broken indicated that the glycopolypeptide is highly constrained by intrachain disulfide bonds.
mesh_terms: D002850:Chromatography, Gel; D002942:Circular Dichroism; D004586:Electrophoresis; D006020:Glycopeptides / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins