Description of a suicide technique in vitro for granulocytic stem cells (CFUc) by hydroxyurea on normal human bone marrow.
It has been possible, by studying 15 normal human bone marrow samples, to develop a technique for the suicide effect in vitro by hydroxyurea (H.U.), applied to human granulocytic stem cells (CFUc). This study suggests a contact time of medullary cells from 60 to 80 minutes in the presence of a hydroxyurea concentration 6 mM per 10(6) cells per ml in vitro. Preliminary results concerning the clusters are discussed.
D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002470:Cell Survival / Q000187:drug effects; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects*; D009994:Osmolar Concentration; D013997:Time Factors
D006918:Hydroxyurea