Effects of low concentrations of cetyltrimethylammonium bromide on diluted bovine spermatozoa.
Cetyltrimethylammonium bromide (CTAB) at a concentration of 10 mg/l in a citrate buffer partially protected sperm against the effects of cold shock treatment. CTAB concentrations of 10 and 20 mg/l also increased in vitro sperm livability when semen was diluted in Caprogen and incubated at 37 degrees C (50 h v. 60 and 57 h). Concentrations of CTAB exceeding 50 mg/l reduced in vitro sperm livability. The addition of catalase (4.5 mg/l) to Caprogen produced greater increases in in vitro sperm livability (50 h v. 110 h), but did not eliminate the toxic effect of the higher CTAB concentrations. The addition of CTAB (10 mg/l) to the Caprogen-catalase diluent significantly increased the non-return rate of the diluted semen in only one of four large-scale field trials and did not alter non-return rates with deep-frozen semen.
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002417:Cattle / Q000502:physiology*; D002470:Cell Survival; D002593:Cetrimonium Compounds / Q000494:pharmacology*; D003080:Cold Temperature; D005615:Freezing; D008297:Male; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012661:Semen; D013094:Spermatozoa / Q000187:drug effects*
D002593:Cetrimonium Compounds; D000644:Quaternary Ammonium Compounds; D002374:Catalase