The effects of glycerol removal on cation concentration and morphology of chicken spermatozoa.
Cation concentrations and ultrastructural morphology were used to determine the effects of glycerol removal on chicken spermatozoa. The addition of 15% glycerol decreased magnesium and potassium concentrations of the spermatozoa. Glycerol removal by either centrifugation and resuspension or dialysis did not restore cation levels to the values obtained with untreated spermatozoa. The glycerol-treated spermatozoa appeared normal after glycerol addition but were bent or coiled after both glycerol removal procedures.
D000818:Animals; D002118:Calcium / Q000032:analysis; D002645:Chickens / Q000502:physiology*; D004573:Electrolytes / Q000032:analysis*; D005990:Glycerol / Q000494:pharmacology*; D008274:Magnesium / Q000032:analysis; D008297:Male; D011188:Potassium / Q000032:analysis; D013094:Spermatozoa / Q000032:analysis / Q000187:drug effects / Q000648:ultrastructure*
D004573:Electrolytes; D008274:Magnesium; D005990:Glycerol; D011188:Potassium; D002118:Calcium