Kinetic and metabolic studies on the priming effect of interferon in L cells.
In cultures primed by interferon pretreatment before stimulation by polyriboinosinic-polyribocytidylic acid interferon was detected one hour earlier, its production followed and enhanced pattern and became resistant to actinomycin D 30 min sooner than in unprimed cultures. The kinetics of development of the primed state was found to be a time-and dose-dependent phenomenon. The continuous presence of interferon during the pretreatment period was not required for the development of the primed state. Actinomycin D at a concentration inhibitory for nuclear RNA synthesis did not influence the development of priming. Higher concentrations of the drug and long term alpha-amanitin or cycloheximide pretreatments, inhibitory for heterogeneous nuclear RNA synthesis, prevented the establishment of the primed state.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007369:Interferon Inducers; D007372:Interferons / Q000096:biosynthesis*; D007739:L Cells / Q000187:drug effects; D051379:Mice; D011070:Poly I-C / Q000494:pharmacology*; D013995:Time
D000546:Amanitins; D007369:Interferon Inducers; D003609:Dactinomycin; D007372:Interferons; D003513:Cycloheximide; D011070:Poly I-C