[Photometric micro-determination of bilirubin in capillary plasma of newborns (author's transl)].
The determination of bilirubin in capillary plasma of 52 newborns was carried out with the diazo method according to Jendrassik and Grof and with two bilirubin spectrophotometers (BB-meter model 468 and Bilimeter). The results obtained by these instruments are correlated with those obtained by the diazo method. As far as the influence of hemolysis on the direct photometric analysis with the Bilimeter is concerned, up to 300 mg hemoglobin/100 ml can be compensated in the range of 20 mg bilirubin/100 ml, and up to 600 mg hemoglobin in the range of 15 mg bilirubin/100 ml. Increasing hemoglobin concentrations cause an elevation of the bilirubin data. However, in the range of 10 mg bilirubin/100 ml, hemoglobin concentrations greater than 300 mg/100 ml cause a decrease of the bilirubin data. The precision in the series is less than 3%. A linear relationship was found in the range of 2.5-20 mg bilirubin/100 ml.
D001663:Bilirubin / Q000097:blood*; D002196:Capillaries / Q000378:metabolism*; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D007231:Infant, Newborn; D008846:Micromanipulation / Q000379:methods; D010783:Photometry / Q000379:methods
D006454:Hemoglobins; D001663:Bilirubin
Die photometrische Mikrobestimmung von Bilirubin im unverdünnten Kapillarplasma von Neugeborenen.