Bacterial flora of the small intestine and bile acid metabolism in patients with hepatico-jejunostomy Roux-en-Y.
Duodenal and jejunal bacterial flora and bile acid metabolism were investigated in 14 patients with hepatico-jejunostomy Roux-en-Y. Anaerobic culture procedures were based on the use of a glove-box with an oxygen-free atmosphere and pre-reduced, anaerobically sterilized media. Anaerobic transport was based on evacuation of atmospheric air from transport-tubes and transport-time less than 30 minutes. Bile acid metabolism was evaluated from concentrations of toal bile acids and deconjugated bile acids, glycine/taurine ratio and breath test. In 92 per cent of the patients an abnormal flora-containing anaerobic and/or aerobic bacteria was found in the most proximal part of the jejunum at the site of the entero-entero anastomosis (cf. Fig. 1 and Table 1). Total bile acid concentrations were low in half of the patients, whereas deconjugated bile acids or elevated glycine/taurine ratio was found in one patient only (cf. Table II). An abnormal breath test was found in 5 patients, but without any clear correlation between the breath test and the bacterial flora.
D000328:Adult; D000368:Aged; D001419:Bacteria / Q000302:isolation & purification*; D001647:Bile Acids and Salts / Q000378:metabolism*; D005260:Female; D006500:Hepatic Duct, Common / Q000601:surgery*; D006801:Humans; D007421:Intestine, Small / Q000382:microbiology*; D007583:Jejunum / Q000601:surgery; D008297:Male; D008722:Methods; D008875:Middle Aged
D001647:Bile Acids and Salts