[Phosphoric acid ester poisoned rats after antidote therapy. 2. Determination of serum enzyme activity].
Paraoxon in doses of one LD50 (0.426 mg/kg), eight times and eighty times LD 50 was applied s.c. to female Sprague-Dawley rats. After 3, 6, 10, 24 and 36--48 h the activities of enzymes GOT, GPT, GLDH, SDH, CPK and ChE were measured, once after i.m. antidote application of Toxogonin only, of Toxogonin + atropine and the next one after application of combination Toxogonin + atropine + Solcoseryl (low-molecular components of deproteinized blood from young calves. The values obtained showed that in spite of treatment with Toxogonin or Toxogonin + atropine the activities of the enzymes increased; this enhancement could be prevented by addition of Solcoseryl to Toxogonin + atropine. The ChE-activity after 36 h was equivalent to that of the control value. The effect of paraoxon in the initial phase of poisoning was discussed in connection with hypoxia and acidosis resulting from a respiratory insufficiency as well as the inhibition of ATPase-activity with restriction of the energy metabolism following: consequently the effect of Solcoseryl was interpreted as an activation of the disturbed energy metabolism.
D000110:Acetylcholinesterase / Q000097:blood; D000183:Actihaemyl / Q000494:pharmacology*; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D004798:Enzymes / Q000097:blood*; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007928:Lethal Dose 50; D010261:Paraoxon / Q000506:poisoning*; D051381:Rats; D013385:Succinate Dehydrogenase / Q000097:blood; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology*
D004798:Enzymes; D014020:Tissue Extracts; D000183:Actihaemyl; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase; D000110:Acetylcholinesterase; D010261:Paraoxon
Befunde an Phosphorsäureester-vergifteten Ratten nach Antidottherapie. II. Aktivitätsbestimmungen einiger Serumenzyme.