A method of increasing EA positive cells in mouse spleen lymphocytes.
Treatment of mouse spleen lymphocytes with a high concentration of trypsin (1 mg/ml) increases the number of EA positive cells to include nearly all the B lymphocytes. It seems that all B lymphocytes express Fc receptors but in some cells these are hidden by other proteins present on the cell surfaces.
D000818:Animals; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011402:Pronase / Q000494:pharmacology; D012397:Rosette Formation / Q000379:methods*; D013154:Spleen / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D014357:Trypsin; D011402:Pronase