A sensitive radioimmunoassay for a component of mouse casein.
Mouse casein (m.w. 22,000 daltons) has been purified by employing Sephadex G-100 and DEAE-cellulose column chromatographies. A sensitive radioimmunoassay method has been developed by using [125I]-labelled casein and antiserum elicited in rabbits after injection of glutaraldehyde-treated casein. The assay method is capable of detecting as little as 0.1 ng of casein. The use of the present radioimmunoassay method in detecting casein production in cultured mouse mammary explants has also been demonstrated.
D000818:Animals; D001667:Binding, Competitive; D002364:Caseins / Q000276:immunology / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D005260:Female; D007106:Immune Sera; D008321:Mammary Glands, Animal; D051379:Mice; D011388:Prolactin / Q000494:pharmacology; D011863:Radioimmunoassay; D013997:Time Factors
D002364:Caseins; D007106:Immune Sera; D011388:Prolactin