title: Proposals for quality control methods of bacterial vaccines for immunostimulation. II. In vivo and in vitro stimulation of lymphocytes by BCG.
abstract: An in vitro test for measuring the reaction of the draining lymph node after BCG vaccination and an in vitro test based on the stimulation of lymphocytes by concanavalin A were used to demonstrate immunostimulating properties of different BCG vaccines. A marked difference was seen between vaccines grown as a surface pellicle and ground in a ball mill, and those which were prepared from dispersed homogeneous cultures. In both systems the ball mill preparations were the less active.
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000494:pharmacology* / Q000592:standards; D003208:Concanavalin A / Q000494:pharmacology; D008198:Lymph Nodes / Q000187:drug effects / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D011786:Quality Control; D013936:Thymidine / Q000378:metabolism
chemical_list: D001500:BCG Vaccine; D003208:Concanavalin A; D013936:Thymidine