Purification and properties of tyrosyl tRNA synthetase of rat liver.
Purification of rat liver tyrosine tRNA synthetase yields two protein fractions A and B and both fractions are required for charging of tyrosine to tRNAtyr. Fraction B catalyzes the activation of tyrosine. Fractions A and B have been purified to near homogeneity and they are composed of single polypeptide chains of 62,000 daltons each. Gel filtration studies suggest a molecular weight of 120,000 for the synthetase.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification; D051381:Rats; D013329:Structure-Activity Relationship; D014448:Tyrosine-tRNA Ligase / Q000302:isolation & purification*
D010455:Peptides; D000604:Amino Acyl-tRNA Synthetases; D014448:Tyrosine-tRNA Ligase