[Histochemical experiments on the action of clenbuterol on tracheal mucosa (author's transl)].
The effect of 4-amino-alpha-[(tert.-butylamino) methyl]-3,5-dichlorobenzyl alcohol hydrochloride (clenbuterol, Spiropent) on the tracheal mucosa was examined in 56 guinea pigs after i.p. administration during 5, 12 and 28 days, respectively. The alkaline phosphatases showed a minimum increase only in long-term tests. This increase concerned mainly the capillaries. It can be interpreted as a sign of enhanced transport processes. After administration of clenbuterol the total acid phosphatase in the mucosal epithelium rose. After a transient apical secretion of enzymes--together with consumption of enzymes at increased secretion--apparently more of new enzymes are produced. As a lysosomal enzyme the glucosaminidase showed also a similar reaction as did the acid phosphatase, while non-specific esterases increased only minimally or moderately. The effect of clenbuterol decreased the number of stainable goblet cells of the mucosa. It came to a secretory evacuation as a result of the secretolytic effect of the test substance in secretory evacuation. At the same time, the part of acid mucus components containing neuraminic acid rose so much that a qualitative change in the sense of the mucus components shifting in the direction to neuraminidasesensitive acid glycosaminoglycanes could be demonstrated.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002976:Clenbuterol / Q000494:pharmacology*; D004983:Ethanolamines / Q000494:pharmacology*; D006168:Guinea Pigs; D006651:Histocytochemistry; D009092:Mucous Membrane / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D014132:Trachea / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism
D004983:Ethanolamines; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000118:Acetylglucosaminidase; D002976:Clenbuterol
Experimentelle histochemische Untersuchungen zur Wirkung von Clenbuterol auf die Trachealschleimhaut.