Effect of a low dose of vincristine on platelet production in mice.
The effect of a single low dose (0.1 mg/kg) of vincristine (VCR) on platelet production was investigated in C57B1 mice. A parallel increase of circulating platelets and (75Se)-selenomethionine (Se-Met) uptake was observed. The total megakaryocyte count decreased insignificantly 6 hours after VCR injection, followed by an increase after 18 hours. Plasma taken from mice 24 hours after VCR injection was tested for thrombopoietic activity. The post-VCR plasma caused a significant thrombocytosis, increased Se-Met uptake and increased protein synthesis of the platelets, indicating an active overproduction of platelets. These results suggest that the thrombocytosis induced by a low dose of VCR is mediated by a thrombopoietin-like substance.
D000818:Animals; D001772:Blood Cell Count; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D001798:Blood Proteins / Q000096:biosynthesis; D006410:Hematopoiesis / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D008533:Megakaryocytes / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D012645:Selenomethionine / Q000378:metabolism; D013922:Thrombocytosis / Q000139:chemically induced; D014750:Vincristine / Q000008:administration & dosage / Q000494:pharmacology*
D001798:Blood Proteins; D014750:Vincristine; D012645:Selenomethionine