Evaluation and improvement of different methods for the enrichment of antigen-binding cells of chickens.
Antigen-binding cells were enriched using bovine serum albumin immunized chickens as an experimental model. A comparison of density centrifugation, adherence to derivatized surfaces (polystyrol and gelatin) and affinity chromatography revealed that optimal enrichment was obtained using the latter procedure. Polyacrylamide was superior to agarose.
D000818:Animals; D000941:Antigens; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D002645:Chickens / Q000276:immunology*; D002846:Chromatography, Affinity; D006385:Hemagglutination Inhibition Tests; D008214:Lymphocytes / Q000276:immunology; D012397:Rosette Formation; D012710:Serum Albumin, Bovine; D013154:Spleen / Q000276:immunology
D000941:Antigens; D012710:Serum Albumin, Bovine