Ornithine decarboxylase activity in cultured endothelial cells stimulated by serum, thrombin and serotonin.
Ornithine decarboxylase (ODC) activity, the rate-limiting step in the synthesis of polyamines, can be demonstrated in cultured, bovine, aortic endothelial cells (EC). Serum, serotonin and thrombin produce a rise in ODC activity. The serotonin-induced ODC activity is significantly blocked by imipramine (10(-5) M) or Lilly 110140 (10(-6) M). Preincubation of EC with these blockers together almost completely depresses the 5-HT-stimulated ODC activity. These observations suggest a manner by which platelets may maintain EC structural and metabolic soundness.
D000818:Animals; D001011:Aorta / Q000166:cytology; D002262:Carboxy-Lyases / Q000378:metabolism*; D002417:Cattle; D004727:Endothelium / Q000201:enzymology*; D007099:Imipramine / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
D012701:Serotonin; D013917:Thrombin; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D007099:Imipramine