Preparation of various Tc-99m dimercaptosuccinate complexes and their evaluation as radiotracers.
The organ distributions of four different Tc-99m dimercaptosuccinate complexes ("Tc-DMS", Complexes 1 to 4) were determined using mice and were evaluated as renal imaging agents. The highest kidney uptake was observed with Complex 2:21.7% of dose, 3 hr after injection. The biologic distributions and gel chromatographic analyses using carrier Tc-99 and Sn-113 indicate that there is little possibility of mixed metal complexes of the type Tc-Sn-DMS: rather they contain only Tc + DMS. The labeling procedure for Tc-99m DMS as a renal agent proceeds in two steps: a rapid formation of Complex 1, and a slower, rate-determining step from Complex 1 to Complex 2. A reproducible lyophilized kit has been prepared. The yield of complex 2 greatly depends on the reconstitution volume of 99mTcO4-; yield averages 89% using 2 ml of 99mTcO4-eluate.
D000818:Animals; D002712:Chlorides; D002850:Chromatography, Gel; D005612:Freeze Drying; D007553:Isotope Labeling; D007668:Kidney / Q000000981:diagnostic imaging / Q000378:metabolism; D008297:Male; D051379:Mice; D011868:Radioisotopes; D011877:Radionuclide Imaging; D051381:Rats; D013667:Technetium; D014001:Tin; D014018:Tissue Distribution
D002712:Chlorides; D011868:Radioisotopes; D013667:Technetium; D014001:Tin