Rat hepatocyte primary cell cultures. III. Improved dissociation and attachment techniques and the enhancement of survival by culture medium.
The conditions for obtaining representative, adult rat hepatocyte primary cell cultures were improved such that viable yields of 50% of the liver were produced which gave rise to cultures representing 30% of the liver. The survival of these cultures in various media was compared revealing that in complex media, particularly containing galactose, survival was improved.
D000818:Animals; D002448:Cell Adhesion; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D005690:Galactose / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver; D010477:Perfusion; D051381:Rats
D003470:Culture Media; D005690:Galactose