[Fluorimetric determination of tranylcypromine in plasma as its 1-dimethylamino-naphthaline-5-sulfonic acid chloride by direct measurement of TLC-plates (author's transl)].
A fluorimetric method for analysis of tranylcypromine from plasma is described. After extraction from plasma tranylcypromine reacts with 1-dimethylamino-naphthaline-5-sulfonic acid chloride (DANS-Cl) in order to form the highly fluorescent DANS-tranylcypromine. After chromatographic separation on silicagel plates quantitation is achieved by in situ fluorescence determination. The recovery of tranylcypromine from plasma is 64% with a coefficient of variance of about +/- 6%.
D000328:Adult; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds; D006801:Humans; D007700:Kinetics; D008722:Methods; D013050:Spectrometry, Fluorescence; D014191:Tranylcypromine / Q000097:blood*
D003619:Dansyl Compounds; D014191:Tranylcypromine
Fluorimetrische Bestimmung von Tranylcypromin in plasma als 1-Dimethylamino-naphthalin-5-sulfonsäure-Derivat durch direkte quantitative Dünnschichtchromatographie.