Unsaturated and cobalamin saturated transcobalamin I and II in normal human plasma.
Insolubilized antibody against human transcobalamin I has been used as a specific and precise tool for the separation of transcobalamin I (and III) from transcobalamin II. Range and mean (in parentheses) for the unsaturated binding capacity for twenty samples are 40-190 (90) pmol/l and 220-1170 (560) pmol/l for transcobalamin I (and III) and transcobalamin II, respectively. The similar figures for the cobalamin saturated transcobalamins are 200-549 (320) pmol/l and 75-475 (160 pmol/l. On analyses of the cobalamins attached to each of the transcobalamins, it is shown that methylcobalamin accounts for most of the cobalamins attached to transcobalamin I whereas transcobalamin II carries most of the 5'-deoxyadenosylcobalamin.
D000906:Antibodies; D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay; D014155:Transcobalamins / Q000276:immunology / Q000378:metabolism*; D014805:Vitamin B 12 / Q000378:metabolism*
D000906:Antibodies; D001798:Blood Proteins; D014155:Transcobalamins; D014805:Vitamin B 12