Isolation and structural characterization of alkali-labile oligosaccharides from bovine milk-fat-globule membrane.
Treatment of sialoglycopeptides derived from bovine milk-fat-globule membrane with alkaline borohydride released a reduced oligosaccharide fraction from which a tetrasaccharide and two trisaccharides were isolated. Periodate-oxidation studies coupled with methylation analysis and g.l.c.-mass spectrometry established their structures as: N-acetylneuraminyl-(2 leads to 3)-beta-D-galactopyranosyl-(1 leads to 3)-[N-acetylneuraminyl-(2 leads to 6)]-N-acetyl-D-galactosaminitol, N-acetylneuraminyl-(2 leads to 3)-D-glactopyranosyl-(1 leads to 3)-N-acetyl-D-galactosaminitol and beta-D-galactopyranosyl-(1 leads to 3)-[N-acteylneuraminyl-(2 leads to 6)]-N-acetyl-D-galactosaminitol respectively.
D000818:Animals; D001894:Borohydrides; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D008566:Membranes / Q000032:analysis; D008892:Milk / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification; D012795:Sialoglycoproteins / Q000032:analysis
D001894:Borohydrides; D009844:Oligosaccharides; D012795:Sialoglycoproteins