Effects of cycloheximide on the ultrastructure of the zona fasciculata of the young rat adrenal.
The ultrasturctural changes induced in the cells of the young rat adrenal zona fasciculata by cycloheximide are studied. Thirty minutes after administration of doses producing inhibition of protein synthesis (1.5 and 4.5 mg/kg body weight) there was a significant increase in the relative volume of the nucleolus, as shown by stereologic methods, which was accompanied by extensive perinucleolar chromatin caps. In the cytoplasm, membranous whorls were observed in several organelles and the background cytoplasm, but the volume fractions of the organelles were not significantly altered. Nucleolar changes were interpreted as suggesting that the inhibition of cytoplasmic protein synthesis may be accompanied by an enhanced formation of nucleolar proteins.
D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure; D003513:Cycloheximide / Q000494:pharmacology*; D003593:Cytoplasm / Q000648:ultrastructure; D014176:Protein Biosynthesis; D051381:Rats
D002843:Chromatin; D003513:Cycloheximide