title: Effect of irradiation on the erythrocyte membranes of homozygous Gunn rat sucklings.
abstract: Erythrocyte membranes were isolated from irradiated Gunn's strain of jaundiced rat sucklings, and photodynamic action of bilirubin in vivo was investigated using sodium dodecyl sulfate-polyacrylamide-gel electrophoresis. The effectiveness of irradiation was verified in terms of decrease of plasma bilirubin concentrations and cerebellar bilirubin contents, and also by recovery from the cellbellar hypoplasia, a characteristic feature of the homozygotes (j/j). The cross-linking of erythrocyte membrane proteins by photodynamic action of bilirubin was demonstrated in vitro in rat erythrocytes as reported for human erythrocytes by Girotti (Biochemistry 14: 3377, 1975). The phenomenon, however, was not observed in vivo. Instead, a protein band with a mol. wt. of 32,000 was increased by the irradiation both in the homozygous (j/j) and heterozygous (j/+) Gunn rats.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001663:Bilirubin / Q000097:blood / Q000378:metabolism; D001921:Brain / Q000528:radiation effects; D002531:Cerebellum / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000528:radiation effects*; D004912:Erythrocytes / Q000528:radiation effects*; D006720:Homozygote; D006801:Humans; D007231:Infant, Newborn; D007567:Jaundice, Neonatal / Q000378:metabolism / Q000628:therapy*; D008565:Membrane Proteins / Q000378:metabolism; D010789:Phototherapy; D051381:Rats
chemical_list: D008565:Membrane Proteins; D001663:Bilirubin