title: Medrogestone: analysis in serum by high-pressure liquid chromatography.
abstract: A method for the determination of 6,17-dimethylpregna-4,6-diene-3,20-dione (medrogestone) in serum is described using high-pressure liquid chromatography with UV detection. Interfering substances in human or dog serum blanks were removed selectively by reaction with oxalyl chloride followed by an aqueous alkaline borate "wash" of the extract. The method is specific for medrogestone and, based on 5 ml serum, has a limit of detection of 2 ng/ml.
mesh_terms: D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D008297:Male; D008524:Medrogestone / Q000097:blood*; D008722:Methods; D011245:Pregnadienes / Q000097:blood*; D013997:Time Factors
chemical_list: D011245:Pregnadienes; D008524:Medrogestone