[Effect of the type of extraction solution and length of storage on the distribution of lactate dehydrogenase isoenzymes in piglet liver homogenate].
Type of extraction solution applied to preparation of homogenate of piglet liver is of negligible importance, if immediately follows electrophoretic separation of tissue material. --Storage of liver homogenates in frozen state over 30 days involves insignificant changes of distribution of LD isoenzymes in 0.25 M sucrose, distinct changes in 0.1 M Tris-buffer, pH 7,5, and important ones in mercaptoaethanol-containing extraction solution, applied to preparation of tissue homogenates.
D000818:Animals; D066298:In Vitro Techniques; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D013552:Swine / Q000378:metabolism*; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase
Der Einfluss der Art des Extraktionsmittels und der Dauer der Aufbewahrung auf die Verteilung der LDH-Isoenzyme im Homogenat der Ferkelleber.