Delta-aminolevulinic acid dehydratase--a sensitive indicator of lead exposure in Japanese quail.
Red blood cell delta-aminolevulinic acid dehydratase (RBC-ALAD) activity has proven to be a sensitive indicator of lead exposure in humans. The depressed enzyme activity and its negative correlation to blood lead concentrations are well-known effects of lead exposure in man. The sensitivity of RBC-ALAD activity in young Japanese quail exposed to low levels of lead as lead acetate was investigated. Two groups of nine birds each were fed purified diets containing either no added lead or 25 micrograms of lead per g. of diet. After 2 weeks, blood samples for hematocrit, hemoglobin, and enzyme determinations and renal, hepatic, duodenal, and tibial tissues were collected. There were no significant differences between controls and lead-fed birds in body, kidney, duodenal, and tibial weights, or in hematocrit and hemoglobin concentrations. However, the renal, hepatic, duodenal, and tibial lead concentrations were significantly (P less than 0.001) greater in the lead-treated birds. The activity of RBC-ALAD in the group fed lead was 45% of that in the control group; these values were significantly different (P less than 0.001). RBC-ALAD activity expressed as the log. base 10 showed significant (P less than 0.02) negative correlation with both hepatic and tibial lead. The study demonstrates that the activity of RBC-ALAD in the Japanese quail is a very sensitive indicator of lead exposure.
D000818:Animals; D003370:Coturnix / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D007668:Kidney / Q000378:metabolism; D007854:Lead / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D000623:Porphobilinogen Synthase / Q000097:blood*; D011784:Quail / Q000378:metabolism*; D013977:Tibia / Q000378:metabolism
D007854:Lead; D000623:Porphobilinogen Synthase