Creatine phosphokinase activity in dysgenic (mdg/mdg) mouse muscle.
The specific activity of creatine phosphokinase (CPK) was measured in the muscle of mdg/mdg and control embryos of 14-18 days' gestation. CPK specific activity values were similar in mutant and normal embryos at the earliest stages examined (14-15 days). However, after 15 1/2 days, the specific activity of the enzyme in the mdg/mdg embryos was approximately 50% lower than in the controls. The dysgenic and normal muscle extracts exhibited comparable stability after storage at -85 C. CPK activity levels in the muscle of adult heterozygotes (+/mdg) and wild-type (+/+) controls were found to be statistically identical. The findings suggest that the mdg mutation does not have a primary or direct effect on CPK activity.
D000818:Animals; D003402:Creatine Kinase / Q000235:genetics* / Q000378:metabolism; D004355:Drug Stability; D004622:Embryo, Mammalian; D005260:Female; D005804:Genes, Lethal; D005808:Genes, Recessive; D006579:Heterozygote; D006720:Homozygote; D051379:Mice; D009132:Muscles / Q000201:enzymology*; D011247:Pregnancy
D003402:Creatine Kinase