Mammary gland prolactin receptor and pituitary prolactin secretion in lactating mice with different lactational performance.
SHN female mice, a high mammary tumour strain, are superior to SLN, a low mammary tumour strain, in lactational performance. Mammary gland prolactin receptor and pituitary prolactin secretion during lactation were compared between these strains. The binding activity, the number of receptor sites per mg tissue and the association constant were measured by the in vitro incubation of mammary gland slices with 125I-labelled bovine prolactin, and the pituitary and plasma levels of prolactin were assayed by homologous radioimmunoassay. There was only a slight difference between strains in any of the parameters for prolactin receptor and for prolactin secretion on either day 4 or day 9 of the first lactation. Almost all the correlation coefficients between each parameter for prolactin receptor and the pituitary or plasma level of prolactin were not statistically significant. These findings suggest that any parameter for prolactin examined in this study is not always directly indicative of lactational performance and further show that the individual variation in the pituitary prolactin secretion during lactation is not so great as to alter the prolactin receptor.
D000818:Animals; D001940:Breast / Q000378:metabolism*; D005260:Female; D007774:Lactation; D051379:Mice; D008815:Mice, Inbred Strains; D010298:Parity; D010902:Pituitary Gland / Q000378:metabolism*; D011247:Pregnancy; D011388:Prolactin / Q000378:metabolism; D011955:Receptors, Drug
D011955:Receptors, Drug; D011388:Prolactin