title: Agarose microdroplet leucocyte migration technique for detection of cell-mediated hypersensitivity to PPD and renal carcinoma antigen.
abstract: The agarose microdroplet leucocyte migration technique is an in vitro technique for detection of cell-mediated hypersensitivity in man, using only 10-15% of the volume of blood required for other leucocyte migration techniques. The technique is described in detail and results are presented from migrations with leucocytes from hypernephroma patients and controls tested with hypernephroma extract and normal kidney extract, and from tuberculin-positive and tuberculin-negative persons tested with PPD.
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology; D000368:Aged; D000951:Antigens, Neoplasm; D002464:Cell Migration Inhibition / Q000379:methods*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007680:Kidney Neoplasms / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012685:Sepharose; D014373:Tuberculin / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D014373:Tuberculin; D012685:Sepharose