title: Rat hepatocyte primary cell cultures. III. Improved dissociation and attachment techniques and the enhancement of survival by culture medium.
abstract: The conditions for obtaining representative, adult rat hepatocyte primary cell cultures were improved such that viable yields of 50% of the liver were produced which gave rise to cultures representing 30% of the liver. The survival of these cultures in various media was compared revealing that in complex media, particularly containing galactose, survival was improved.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D005690:Galactose / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver; D010477:Perfusion; D051381:Rats
chemical_list: D003470:Culture Media; D005690:Galactose