A convenient method for the isolation of 3'-sialyllactose from normal human urine.
A simple method for the preparation of 3'-sialyllactose from normal human urine is described. The method for the collection of sialooligosaccharides is based on adsorption on charcoal followed by elution with a mixture of ethanol, pyridine, and water. The sialooligosaccharide mixture is then fractionated by means of gel filtration on Sephadex G-25 and the low molecular weight fraction is further fractionated on Dowex 1 (eluted with pyridinium acetate) to give five fractions. The last fraction contains almost pure 3'-sialyllactose.
D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D006801:Humans; D007785:Lactose / Q000031:analogs & derivatives* / Q000652:urine; D012794:Sialic Acids / Q000652:urine*
D012794:Sialic Acids; D007785:Lactose