title: Specificity of Hpa II and Hae III DNA methylases.
abstract: The methylases M.HaeIII and M.HpaII recognize the tetranucleotide sequences [Formula: see text] and [Formula: see text] respectively, in DNA, and transfer a methyl group from S-adenosylmethionine to the 5-position of cytosine on each strand as indicated by the asterisks. Restriction endonuclease R.HaeIII does not cleave the methylated sequence [Formula: see text] but can cleave [Formula: see text] in which methylation is introduced on the unnatural external cytosine positions. Similarly, R.HpaII does not cleave [Formula: see text] but can cleave [Formula: see text].Images
mesh_terms: D001483:Base Sequence; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes; D006190:Haemophilus / Q000201:enzymology*; D008780:Methyltransferases; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D013045:Species Specificity; D013379:Substrate Specificity
chemical_list: D009838:Oligodeoxyribonucleotides; D008780:Methyltransferases; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes