An improved method for purification of lymphocytes.
A method that combines glass-bead column filtration, Ficoll-Hypaque gradient separation, discontinuous sucrose gradient, and drastic reduction of cell transfers is described. The procedure gives a high yield of pure human lymphocytes from small amounts of blood, good preservation of B cell/T cell ratio, and sufficient material for subsequent biochemical studies.
D000293:Adolescent; D002469:Cell Separation / Q000379:methods; D002498:Centrifugation; D002648:Child; D005374:Filtration; D005898:Glass; D006801:Humans; D008214:Lymphocytes