Griseofulvin-induced aggregation of microtubule protein.
Griseofulvin (7-chloro-2',4,6-trimethoxy-6'-methylspiro[benzofuran-2(3H),1'-[2]cyclohexene]-3,4'-dione) induces aggregation of microtubule protein at 0 degrees C. This aggregate contains approx. 90% of the microtubule-associated proteins originally present in the microtubule protein. The supernatant obtained after removal of the griseofulvin-induced aggregate does not form microtubules on warming at 37 degrees C. Addition of the griseofulvin-aggregated protein to this supernatant and warming to 37 degrees C gives rise to a limited amount of microtubule assembly. The possible involvement of griseofulvin-induced aggregation of microtubule protein at 0 degrees C in the inhibition by griseofulvin of microtubule assembly in vitro is discussed.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins; D006118:Griseofulvin; D046911:Macromolecular Substances; D008870:Microtubules; D009391:Nephelometry and Turbidimetry; D012756:Sheep; D013696:Temperature; D014404:Tubulin
D006023:Glycoproteins; D046911:Macromolecular Substances; D014404:Tubulin; D006118:Griseofulvin