[9-(Adenylyl)alkylcobalamins as inhibitors of adenosylcobalamin-dependent glycerol dehydratase from Aerobacter aerogenes].
The behavior of two coenzyme analogs, [(5-aden-9-yl)methoxyethyl] cob (III) alamin and [(5-aden-9-yl)pentyl] cob (III) alamin modified at the nucleoside ligand sugar moiety was studied in the system of adenosyl-cobalamin-dependent glycerol dehydratase from Aerobacter aerogenes. It was shown that neither of the analogs possesses coenzyme properties and that both are strong competitive inhibitors for adenosylcobalamin (AdoCbl). The affinity of the two analogs for the apoenzyme is higher than that of AdoCbl. The data obtained are indicative of the essential role of the ribofuranoside fragment of AdoCbl in the manifestation of the coenzyme activity. The apoenzyme interaction with the analogs under study is discussed in terms of the Dreiding stereomodels for AdoCbl and its analogs.
D001051:Apoenzymes / Q000378:metabolism; D003067:Coenzymes / Q000378:metabolism*; D004754:Enterobacter / Q000201:enzymology*; D004755:Enterobacteriaceae / Q000201:enzymology*; D005990:Glycerol; D006836:Hydro-Lyases / Q000037:antagonists & inhibitors*; D013329:Structure-Activity Relationship; D014805:Vitamin B 12 / Q000378:metabolism* / Q000494:pharmacology
D001051:Apoenzymes; D003067:Coenzymes; D006836:Hydro-Lyases; C020671:glycerol dehydratase; D014805:Vitamin B 12; D005990:Glycerol
9-(Adenilil)Alkilkobalaminy kak ingibitory adenozilkobalamin-zavisimoĭ glitseroldegidratazy iz Aerobacter aerogenes.