The use of filtration techniques for the lysis and study of red blood cells.
A filtration technique for the gentle lysis of erythrocytes has been developed using cellulose triacetate membranes. When cell suspensions are filtered under nitrogen pressure, lysis occurs at the surface of the filter in such a way that the cell ghosts are retained on the filter. The contents of the cell are extruded through the pores of the filter without mixing with the cell suspension. Cell ghosts and intact erythrocytes have been collected on membranes and examined by electron microscopy. These preparations have the advantage of being free of the structural artifacts that result from centrifugation. In addition, the filter facilitates preparation for electron microscopy by providing a support for the sample during fixation and then dissolving during the dehydration of the sample.
D000818:Animals; D002498:Centrifugation; D002645:Chickens; D004910:Erythrocyte Membrane / Q000648:ultrastructure; D004912:Erythrocytes / Q000648:ultrastructure*; D005374:Filtration / Q000379:methods; D006461:Hemolysis; D006801:Humans