Evaluation of genetic risks of alkylating agents. III. Alkylation of haemoglobin after metabolic conversion of ethene to ethene oxide in vivo.
Male CBA mice, exposed to air contaminated with [14C] labelled ethene, were able to metabolize this olefine to ethene oxide. The amount of epoxide formed was quantitatively determined from the degree of alkylation of cysteine and histidine in haemoglobin. These hydroxyethylated amino acids were determined by ion-exchange chromatography of the labelled products. In a separate experiment the formation of S-(2-hydroxyethyl) cysteine was verified by gas chromatography--mass spectrometry. In addition this cysteine derivative was determined in urine by thin-layer chromatography. For unknown reasons, uninduced mice varied strongly in the extent to which they converted ethene to epoxide.
D000336:Aerosols; D000393:Air Pollutants / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005027:Ethylene Oxide / Q000494:pharmacology*; D005030:Ethylenes / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009153:Mutagens
D000336:Aerosols; D000393:Air Pollutants; D005030:Ethylenes; D006454:Hemoglobins; D009153:Mutagens; D005027:Ethylene Oxide