Staining of glycosaminoglycans in intervertebral disc cells.
Disc material from horse, ox, sheep, pig, dog and cat was stained by the Alcian-blue-critical electrolyte concentration technique and with the standard and two-step periodic acid Schiff methods. The effects of pretreatment with hyaluronidase and with chondroitinase was also evaluated. There appears to be a small increase in total cellular glycosaminoglycan content with age in all species: cellular material of high molecular weight however only increases in aged animals. The degree of sulphation of cellular glycosaminoglycans does not vary with age or with position in the disc.
D000423:Alcian Blue; D000818:Animals; D002415:Cats; D002417:Cattle; D002810:Chondroitinases and Chondroitin Lyases / Q000494:pharmacology; D004285:Dogs; D006025:Glycosaminoglycans / Q000378:metabolism*; D006651:Histocytochemistry; D006736:Horses; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D007403:Intervertebral Disc / Q000378:metabolism*; D010503:Periodic Acid-Schiff Reaction; D012756:Sheep; D013552:Swine
D006025:Glycosaminoglycans; D006821:Hyaluronoglucosaminidase; D002810:Chondroitinases and Chondroitin Lyases; D000423:Alcian Blue