Affinity chromatography of thiol-containing purines and ribonucleic acid.
Evidence is presented for the specific interaction of 6-mercaptopurine with mercurated cellulose. Following from this, a new method is described for the affinity chromatography of thiol-containing molecules and of RNA containing incorporated 6-thioguanosine on columns of mercurated cellulose. This technique may find application in the study of RNA metabolism and gene expression.
D002482:Cellulose; D002846:Chromatography, Affinity / Q000379:methods; D015122:Mercaptopurine; D008628:Mercury; D012313:RNA / Q000302:isolation & purification*; D013872:Thionucleosides / Q000302:isolation & purification*
D013872:Thionucleosides; D012313:RNA; D002482:Cellulose; D015122:Mercaptopurine; D008628:Mercury