[RNA synthesis in bone marrow hematopoietic stem cells].
The effect of inhibitors of DNA-dependent RNA synthesis (sibiromycin and actinomycin D) and radio toxic doses of 3H-uridine on the bone marrow stem hemopoietic cells in adult mice was studied by means of spleen colonies. The short-term in vitro preincubation of the bone marrow cell suspension with the antibiotics and 3H-uridine resulted in the practically complete inhibition of colony formation. The bone marrow stem hemopoietic cells incorporated 3H-uridine intensively. In the normal adult animals the number of stem cells with the high level of RNA synthesis attained 40% whereas the rest 60% of cells were capable to activate the ribonucleic metabolism upon their in vitro preincubation.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001854:Bone Marrow Cells; D003609:Dactinomycin / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000378:metabolism* / Q000528:radiation effects; D006824:Hybridization, Genetic; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D012313:RNA / Q000096:biosynthesis*; D014316:Tritium / Q000009:adverse effects
D000903:Antibiotics, Antineoplastic; D014316:Tritium; D003609:Dactinomycin; D012313:RNA
Sintez RNK v stvolovykh krovetvornykh kletkakh kostnogo mozga.