Detection of sensitized human blood lymphocytes by agglutination with basic peptides: a possible test for malignant disease.
A simple rapid micro-agglutination test for detecting sensitized lymphocytes from human peripheral blood is described: the poly-L-lysine (PLL) 3400 agglutination test of lymphocytes (PAL test). The incubation of lymphocytes from 30 cancer patients, 30 patients with non-malignant disease and 40 healthy controls with PLL (mol. wt 3400) is evaluated. The test was positive in 83 percent of malignant and 20 percent of non-malignant cases. All healthy controls were negative. Other peptides tested showed no significant difference in reaction between malignant and non-malignant diseases. The mechanism of detection of sensitized lymphocytes from patients with malignant disease through agglutination induced by PLL 3400 is discussed.
D000372:Agglutination Tests; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008970:Molecular Weight; D009369:Neoplasms / Q000175:diagnosis*; D010455:Peptides / Q000494:pharmacology*; D011107:Polylysine / Q000494:pharmacology
D010455:Peptides; D011107:Polylysine