New method for obtaining IgA-specific protease.
A simple method for obtaining an active preparation of IgA-specific protease from a bacterial source is presented. In this method Streptococcus sanguis was inoculated onto the surface of a dialysis membrane on nutrient agar. Following growth, the membrane was removed from the agar surface and washed in a small volume of buffer. A solution with protease activity against IgA1 monoclonal proteins was obtained by clarification of the wash and appeared to be similar to enzyme preparations obtained by other methods.
D000918:Antibody Specificity; D004591:Electrophoresis, Polyacrylamide Gel; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A; D010447:Peptide Hydrolases / Q000276:immunology*; D013298:Streptococcus sanguis / Q000276:immunology
D007070:Immunoglobulin A; D010447:Peptide Hydrolases