Procainized murine macrophages fail in the agglutination of opsonized isologous erythrocytes.
Murine peritoneal macrophages exhibit numerous slender microvilli and several invaginations of the cell membrane. By treatment with isotonic 2.10(-2) M procain-PBS the surface of macrophages is smoothed significantly. Isolated macrophages agglutinate opsonized isologous erythrocytes. Procainizing of macrophages inhibits this agglutination. The finding provides evidence for typing of macrophage receptors whether or not sensitive to procain treatment.
D000371:Agglutination / Q000187:drug effects*; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D004912:Erythrocytes / Q000276:immunology; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D009895:Opsonin Proteins; D011343:Procaine / Q000494:pharmacology*; D051381:Rats
D009895:Opsonin Proteins; D011343:Procaine