Effect of light-adaptation on the photoreaction of bacteriorhodopsin from Halobacterium halobium.
Light-induced formation of the 410 nm intermediate was investigated on dark-and light-adapted bacteriorhodopsin. The amplitude of the light-induced absorption increase at 410 nm of the light-adapted bacteriorhodopsin was twice as large as that of the dark-adapted bacteriorhodopsin. The amount of protons released from bacteriorhodopsin in response to illumination was also enhanced by light-adaptation. The degree of the enhancement was independent of the temperature in the dark-adaptation. The relation between these photochemical events and the isomeric configurations of retinal is discussed.
D000222:Adaptation, Physiological; D001436:Bacteriorhodopsins / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003624:Darkness; D006217:Halobacterium / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D010777:Photochemistry; D012172:Retinaldehyde / Q000378:metabolism; D013237:Stereoisomerism
D002338:Carotenoids; D001436:Bacteriorhodopsins; D012172:Retinaldehyde