title: A technique for the exposure and detection of inhaled aerosols in poultry.
abstract: The respiratory activity of poultry is highly susceptible to changes in respiratory dead space volume, airstream pressure, ambient temperature, and body position. A technique has been developed which will minimize these variables; yet permit the exposure and detection of inhaled aerosols. Monodispersed latex spheres of a known size are radioactively labeled following polymerization of iodine-127 onto the surface of the spheres. The bird is anesthetized and placed in a whole-body plethysmography. The liquid dispersed latex spheres are aerosolized from a nebulizer attached to a 10 p.s.i.g. compressed air line. The particles are electrostatically neutralized and routed to a plenum at near ambient pressure. The bird breathes at will through a unidirectional mask attached to the plenum. Following exposure the bird is euthanatized, the plethysmograph calibrated and the deposition in the respiratory tract measured at each of seven sections by a collimated, scintillation detector.
mesh_terms: D000336:Aerosols; D000818:Animals; D002645:Chickens / Q000502:physiology*; D008297:Male; D008722:Methods; D008863:Microspheres; D011137:Polystyrenes; D012119:Respiration
chemical_list: D000336:Aerosols; D011137:Polystyrenes