title: Study of carboxyl groups on the human blood platelet membrane.
abstract: The authors have studied the whole carboxyl groups of the platelet membrane using liquid phase electrophoresis and an initial step of activation of the acid group by a water soluble carbodiimide (ethyl-l-diméthyl-aminopropyl-3-carbodiimide), followed by the action of a nucleophilic molecule. The calculation based on the theory of Gouy-Chapman have shown the existence of 24, 7.10(5) groups per cell. That is a number about 2.5 fold superior to the carboxyl groups of sialic acid as determined by the action of neuraminidase.
mesh_terms: D001792:Blood Platelets / Q000032:analysis*; D002264:Carboxylic Acids / Q000097:blood*; D002462:Cell Membrane / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D005022:Ethyldimethylaminopropyl Carbodiimide; D006801:Humans; D012794:Sialic Acids
chemical_list: D002264:Carboxylic Acids; D012794:Sialic Acids; D005022:Ethyldimethylaminopropyl Carbodiimide