title: A procedure for the fusion of cells in suspension by means of polyethylene glycol.
abstract: The nuclei of chick and human fibroblasts are readily distinguishable in Leishman stained heterokaryons. The use of these two types of cell has allowed development of a method for fusion in suspension based on the use of polyethylene glycol (PEG) of MW 1000 in Dulbecco's medium containing 15% dimethylsulfoxide (DMSO).
mesh_terms: D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus; D006801:Humans; D008970:Molecular Weight; D011092:Polyethylene Glycols
chemical_list: D011092:Polyethylene Glycols