[Viability, ATP and 2,3-DPG content of resuspended erythrocytes during several-week storage in cold].
Leukocyte-and thrombocyte-poor packed red cells obtained from ACD or. ACD-AG blood were resuspended to a hematocrit of about 55% and stored at 4 degrees C. The resuspension solution consisted of xylitol, inorganic phosphate, bicarbonate, adenine (A) and guanosine (G) solved in water. In one case glucose, citrate and sucrose were also added, in another one, sorbitol. The 2,3-DPG and the ATP level remained for a longer period in the sorbitol-xylitol-medium than in the glucose-xylitol-medium. The ATP content in the red cell suspension was higher than in packed cells. Higher ATP values were obtained in red blood cells from whole blood with adenine and guanosine. The survival rate of resuspended red blood cells in glucose-AG-citrate-sucrose medium was about 80--85% after 3 weeks of storage and 77% after 6 weeks with a higher range.
D000255:Adenosine Triphosphate / Q000097:blood*; D001793:Blood Preservation; D002470:Cell Survival; D003080:Cold Temperature; D004912:Erythrocytes / Q000378:metabolism*; D005994:Glycerophosphates / Q000097:blood*; D006801:Humans; D013535:Suspensions; D013997:Time Factors
D005994:Glycerophosphates; D013535:Suspensions; D000255:Adenosine Triphosphate
Verhalten von Lebensfähigkeit, ATP-und 2,3-DPG-Gehalt resuspendierter Erythrozyten während mehrwöchiger Kältelagerung.