RNA methylation and control of eukaryotic RNA biosynthesis: processing and utilization of undermethylated tRNAs in CHO cells.
The role of RNA methylations in the control of tRNA production and utilization for protein biosynthesis has been investigated through a study of the effects in vivo of cycloleucine a specific and potent inhibitor of S adenosyl-methionine mediated methylation. During the cycloleucine treatment, the rate of appearance of newly synthetized tRNAs into the cytoplasm is markedly reduced (about 50%). These molecules are extensively (more than 90%) undermethylated and are integrated into polysomes, but at a slower rate than normally methylated tRNAs.
D002460:Cell Line; D003515:Cycloleucine / Q000494:pharmacology; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism*; D012359:tRNA Methyltransferases / Q000378:metabolism*
D003515:Cycloleucine; D012343:RNA, Transfer; D012359:tRNA Methyltransferases