Stereochemical model for proflavin intercalation in A-DNA.
Linked-atom molecular modelling was employed to determine the steric and torsional requirements for intercalation of proflavine into a double-stranded region of DNA compatible with adjacent regions of cohelical A-DNA. The optimum intercalation conformation is characterized by the dihedral angles xi and psi becoming trans, with all sugars retaining the characteristics C3'-endo pucker. This extended conformation results in virtually no helical unwinding, suggesting it may be an appropriate model for an intercalative intermediary in mutagenesis by virtue of its similarity to standard helical DNA.
D000166:Acridines / Q000494:pharmacology*; D004247:DNA; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011370:Proflavine / Q000494:pharmacology*
D000166:Acridines; D004247:DNA; D011370:Proflavine