[Micromethod for studying lipid groups in the serum].
A microanalytical variant for the determination of lipids and lipid groups in serum is described, by the application of chromatographis technique and densitometric measurement of the separated zones. The variant scheme is as follows: lipid extraction, distribution of the extract for determination into total lipids, total cholesterine and chromatographic separation of the lipid mixture. Sulphonamic acid, embeded in the layer is used in the carbonization of the zones from the chromatogram. The conditions of carbonization and densitometry are indicated. The accuracy and reproducibility of the analytical determinations according to the scheme presented meet the requirements. The calculation of the results from the densitogram in the adopted mg per 100 ml is through the usage of test established correction factors. Seven lipid indices could be determined in 0.2 ml serum and manipulation time analysis for 10 samples is two hours. A special advantage of the method proposed is the possibility of accurately studying the dynamic of the lipid group in different morbid states or at experimental work. The method could be adopted, without principal changes for analysis of tissue lipids.
D002855:Chromatography, Thin Layer; D003720:Densitometry; D006801:Humans; D008055:Lipids / Q000097:blood*; D008832:Microchemistry / Q000379:methods*; D013046:Specific Gravity
D008055:Lipids
Mikrometod za izsledvane na lipidni grupi v serum.