title: [GLC method for microdetermination of catecholamines (author's transl)].
abstract: A gas liquid chromatographic method for determination of catecholamines in small sample is described. The method involves homogenization of biological material into 0,1 M formic acid 5 mM ascorbic acid and subsequent conversion in their trimethylsilyl derivatives with BSA reagent and determined on 3% OV-1 column in a isothermal condition at 220 degrees C with the cromatograph equipped with hydrogen flame ionization. With respect to specificity, reproducibility and recovery, the results were satisfactory.
mesh_terms: D002395:Catecholamines / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*
chemical_list: D002395:Catecholamines
vernacular_title: Método de cromatografia a gás para a microdeterminação de catecolaminas.