Auxotrophic mutants in the selection of the rubomycin producer, Actinomyces coeruleorubidus.
A total of 351 auxotrophic mutants with different antibiotic activity, including several mutants with activity higher than that of the parent prototrophic strains were obtained under the effect of gamma-rays from 3 prototrophic strains of Act. coeruleorubidus. It was shown that most of the auxotrophic mutants did not preserve the property of biochemical insufficiency on passages on complete media. A mutant strain 1059-32 with activity 2 times higher than that of the prototrophic strain 2-39 and the parent auxotrophic culture was obtained from the revertants. Requirements in 29 growth factors including 17 amino acids, 4 nitrous bases, 8 vitamins and coenzymes were determined in 46 stable auxotrophic mutants isolated. The effect of the specific and non-specific growth factors on the culture antibiotic production was studied.
D003037:Cobalt Radioisotopes; D003470:Culture Media; D003630:Daunorubicin / Q000096:biosynthesis*; D005720:Gamma Rays; D008967:Molecular Biology / Q000528:radiation effects; D009154:Mutation / Q000528:radiation effects*; D012641:Selection, Genetic / Q000528:radiation effects; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D003037:Cobalt Radioisotopes; D003470:Culture Media; D003630:Daunorubicin
Auksotropnye mutanty v selektsii produtsenta rubomitsina----Actinomyces coeruleorubidus.