title: The nucleotide sequence at the termini of adenovirus type 5 DNA.
abstract: The sequences of the first 194 base pairs at both termini of adenovirus type 5 (Ad5) DNA have been determined, using the chemical degradation technique developed by Maxam and Gilbert (Proc. Nat. Acad. Sci. USA 74 (1977), pp. 560-564). The nucleotide sequences 1-75 were confirmed by analysis of labeled RNA transcribed from the terminal HhaI fragments in vitro. The sequence data show that Ad5 DNA has a perfect inverted terminal repetition of 103 base pairs long.
mesh_terms: D000256:Adenoviridae / Q000032:analysis*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D009843:Oligoribonucleotides / Q000032:analysis; D012260:Ribonucleases; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D009843:Oligoribonucleotides; D012260:Ribonucleases; D004262:DNA Restriction Enzymes