In vitro anti-tumour properties of peritoneal exudate cells of conventional germ-free and stimulated mice.
Adherent cells of peritoneal exudates were obtained from conventional, or from germ-free mice or from mice having received in intraperitoneal infection of a variety of phlogogenic substances such as Corynebacterium parvum (C. parvum) (Mérieux) 500 microgram, thioglycolate (Difco) 3 ml, Bayol (Esso) 0.50 ml + 0.50 ml culture medium, glycogene 1.2 ml. The cytotoxic properties adherent cells were studied in vitro by the chromium release technique (CRT) and their cytostatic properties by the inhibition of the incorporation of tritiated thymidine by YC8 lymphoma cells. C. parvum was found to be the most active agent in enhancing the cytotoxic properties of adherent cells, followed by BCG and Bayol. Glycogen peptone and other substrates were without effect. The unstimulated peritoneal macrophages of conventional mice were found to inhibit thymidine incorporation by tumour cells, whereas those of germ-free mice could not do so. C. parvum markedly increased the cytostatic property adhered cells from both germ-free and conventional mice.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology*; D001500:BCG Vaccine; D003601:Cytotoxicity Tests, Immunologic; D005856:Germ-Free Life; D007509:Irritants / Q000008:administration & dosage; D008223:Lymphoma / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000276:immunology; D011425:Propionibacterium acnes / Q000276:immunology; D013213:Starch / Q000276:immunology; D013864:Thioglycolates / Q000276:immunology
D001500:BCG Vaccine; D007509:Irritants; D013864:Thioglycolates; D013213:Starch