title: Purification and properties of the hemagglutinin from Maclura pomifera seeds.
abstract: Maclura promifera seeds contain a protein which agglutinates human erythrocytes at concentrations as low as 4 ng/mL. This property is related to its ability to bind with high specificity various alpha-D-galactopyranosides. The agglutinin, which was pruified by affinity adsorption, exhibits one band on immunoelectrophoresis and displays one peak during ultracentrifugation, isoelectric focusing, and gel permeation chromatography. The active protein has a molecular weight of 40 000-43 000 and contains two dissimilar polypeptide chains of 12 000 and 10 000, respectively.
mesh_terms: D000017:ABO Blood-Group System; D000373:Agglutinins / Q000302:isolation & purification; D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates; D004912:Erythrocytes / Q000276:immunology; D006026:Glycoside Hydrolases / Q000302:isolation & purification; D006388:Hemagglutinins / Q000302:isolation & purification; D006801:Humans; D037102:Lectins / Q000302:isolation & purification; D008970:Molecular Weight; D037121:Plant Lectins; D012639:Seeds / Q000201:enzymology
chemical_list: D000017:ABO Blood-Group System; D000373:Agglutinins; D000596:Amino Acids; D002241:Carbohydrates; D006388:Hemagglutinins; D037102:Lectins; D037121:Plant Lectins; D006026:Glycoside Hydrolases