A procedure for the fusion of cells in suspension by means of polyethylene glycol.
The nuclei of chick and human fibroblasts are readily distinguishable in Leishman stained heterokaryons. The use of these two types of cell has allowed development of a method for fusion in suspension based on the use of polyethylene glycol (PEG) of MW 1000 in Dulbecco's medium containing 15% dimethylsulfoxide (DMSO).
D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus; D006801:Humans; D008970:Molecular Weight; D011092:Polyethylene Glycols
D011092:Polyethylene Glycols