title: Lysostaphin endopeptidase-catalysed transpeptidation reactions of the imino-transfer type.
abstract: The glycylglycine endopeptidase in lysostaphin has been found capable of catalysing both hydrolysis and transpeptidation reactions when acting on glycyl peptides. The ability of the enzyme to utilize dansyldiglycine (5-dimethylaminoaphthalene-1-sulphonylglycylglycine) as an acceptor molecule in transpeptidation reactions, although it is incapable of hydrolysing the peptide bond in this compound, indicates the enzyme must be capable of forming the equivalent of an imino-enzyme intermediate during the catalytic process.
mesh_terms: D003619:Dansyl Compounds; D010450:Endopeptidases / Q000378:metabolism*; D006033:Glycylglycine; D008248:Lysostaphin / Q000378:metabolism*
chemical_list: D003619:Dansyl Compounds; D006033:Glycylglycine; D010450:Endopeptidases; C021524:lysostaphin endopeptidase; D008248:Lysostaphin