Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria.
Poly(A)-containing low molecular weight (7.5S) messenger RNA was isolated in a highly purified form from both polyribosomes and post-polysomal supernatant of rat liver mitochondria. Both mRNA's contain rather short poly(A) tracts (40-70 mononucleotides) according to a profile of their elution from poly(U)-Sepharose column with a gradient of formamide concentration. Both mRNA's when added to a preincubated mitochondrial lysate programmed the synthesis of a hydrophobic polypeptide of a molecular weight about 9000 daltons which was soluble in the neutral chloroform-methanol mixture.
D000818:Animals; D007700:Kinetics; D008930:Mitochondria, Liver / Q000032:analysis*; D008970:Molecular Weight; D011061:Poly A / Q000032:analysis*; D011132:Polyribosomes / Q000032:analysis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D013698:Templates, Genetic
D012333:RNA, Messenger; D011061:Poly A