Sub-fractionation and characterization of lysine-rich histones from Oberling Guerin-Guerin's myeloma.
The H1 fraction in Oberling Guerin-Guerin's myeloma was isolated, and an electrophoretic study was carried out in order to establish its composition. The results obtained show that the H1 fraction is heterogeneous and is formed of 2 sub-fractions. These were separated by preparative gel electrophoresis. They differ in molecular weight, electrophoretic mobility, and primary structure. Each sub-fraction exists in 2 different electric states.
D000818:Animals; D005591:Chemical Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification*; D008239:Lysine / Q000032:analysis; D009374:Neoplasms, Experimental / Q000378:metabolism; D010954:Plasmacytoma / Q000378:metabolism*; D051381:Rats
D006657:Histones; D008239:Lysine