An economic and rapid diagnostic procedure for the detection of salmonella/shigella using the polyvalent salmonella phage O-1.
An easy, rapid and economic two-step procedure is described for the detection of Salmonella/Shigella. In the first step the susceptibility of suspected colonies for the phage O-1 of FELIX and CALLOW is tested. Positive cultures are serologically confirmed. The test is performed on Triple Sugar Iron Agar and lasts 4-6 hrs. Phage negative cultures which are lactose- and sucrose negative are tested for lysine decarboxylase and, if Shigella is possible (i.e. in human material on primary plates), for indol production and motility in a semisolid tryptophane agar. Of 22880 Salmonella straine 21977, i.e. 96.1% were phage-sensitive. Strains belonging to certain O-groups (OE) or species are lysed at a lower percentage. However, since they are lysine decarboxylase positive they are not lost and can be submitted to a serological examination.
D002262:Carboxy-Lyases / Q000378:metabolism; D007211:Indoles / Q000096:biosynthesis; D008239:Lysine; D012475:Salmonella / Q000145:classification / Q000302:isolation & purification*; D012483:Salmonella Phages; D012760:Shigella / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism
D007211:Indoles; D002262:Carboxy-Lyases; D008239:Lysine