Chemical and structural alterations at the cell surface of Candida tropicalis, induced by hydrocarbon substrate.
The surface-localized polysaccharide of alkane-grown cells of Candida tropicalis was identified as mannan containing approximately 4% covalently linked fatty acids. Glucose-grown cells lacked the mannan-fatty acid complex. The surface structure of alkane-grown cells showed a radial arrangement of the wall polymers, with protruding parts. The cell surface of glucose-grown cells was smooth, with a coherent outer limit. The mannan was localized by using concanavalin A. Masking of the mannan with concanavalin A reduced the binding affinity of the surface for alkane, indicating the involvement of the surface-localized mannan-fatty acid complex in the binding of alkanes.
D000473:Alkanes / Q000378:metabolism*; D002175:Candida / Q000378:metabolism* / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D005227:Fatty Acids / Q000378:metabolism; D005614:Freeze Fracturing; D005947:Glucose / Q000378:metabolism; D008351:Mannans / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D011402:Pronase
D000473:Alkanes; D005227:Fatty Acids; D008351:Mannans; D011134:Polysaccharides; D011402:Pronase; D005947:Glucose