Transformation of arachidonic acid into thromboxane B2 by the homogenates of activated macrophages.
The homogenates of activated macrophages obtained from liquid paraffin-injected guinea pig peritoneum were incubated with [14C]arachidonic acid or with radioactive prostaglandin endoperoside [14C]prostaglandin H2. The major radioactive metabolite in both cases was thromboxane B2, which was identified by NaBH4 reduction, rechromatography and autoradiography.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D006168:Guinea Pigs; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D010232:Paraffin / Q000494:pharmacology*; D011463:Prostaglandins H / Q000378:metabolism; D013929:Thromboxane B2 / Q000096:biosynthesis*; D013931:Thromboxanes / Q000096:biosynthesis*
D001095:Arachidonic Acids; D011463:Prostaglandins H; D013931:Thromboxanes; D013929:Thromboxane B2; D010232:Paraffin