Some regulatory properties of purine biosynthesis de novo in long-term cultures of epithelial-like rat liver cells.
De novo synthesis of purine nucleotides and some regulatory properties of this pathway were studied in cultured epithelial-like rat liver cells. It was found that the physiological 5-phosphoribosyl 1-pyrophosphate (P-Rib-PP) concentration in these cells is limited for purine synthesis de novo. Increase of P-Rib-PP availability, achieved by activation of P-Rib-PP synthetase at high Pi concentration, resulted in acceleration of purine synthesis. The effects of increasing cellular ribose 5-phosphate (Rib-5-P) availability, by methylene blue-induced acceleration of the oxidative pentose phosphate pathway, on P-Rib-PP availability and on the rate of the novo purine synthesis were also studied. It was found that at the Pi concentration prevailing in the tissue at extracellular physiological Pi concentration, Rib-5-P availability is saturating for P-Rib-PP generation and therefore also for purine synthesis.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D005561:Formates / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008751:Methylene Blue / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D010754:Phosphoribosyl Pyrophosphate / Q000378:metabolism; D011685:Purine Nucleotides / Q000096:biosynthesis*; D051381:Rats; D012267:Ribosemonophosphates / Q000378:metabolism
D005561:Formates; D010710:Phosphates; D011685:Purine Nucleotides; D012267:Ribosemonophosphates; D010754:Phosphoribosyl Pyrophosphate; D001205:Ascorbic Acid; D008751:Methylene Blue