Isoenzymes of N-acetyl-beta-glucosaminidase from human liver and serum: separation by electrofocusing in thin layers of polyacrylamide gel.
Isoenzymes of N-acetyl-beta-glucosaminidase can be separated by electrofocusing in thin layers of polyacrylamide gel. Patterns of isoenzymes from human liver tissue and serum show changes in liver diseases. Preliminary results indicate, that N-acetyl-beta-glucosaminidase isoenzyme A is increased in liver tissue from patients with chronic liver diseases in relation to the activity of mesenchyme reaction. The described method will be discussed in comparison with known separation techniques.
D000118:Acetylglucosaminidase / Q000097:blood / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006596:Hexosaminidases / Q000302:isolation & purification*; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology*; D008103:Liver Cirrhosis / Q000201:enzymology
D007527:Isoenzymes; D006596:Hexosaminidases; D000118:Acetylglucosaminidase