Bromodeoxyuridine induction of deoxycytidine deaminase activity in a hamster cell line.
The Syrian hamster cell line, RPMI 3460, was found to express barely detectable levels of the enzyme deoxycytidine deaminase. In contrast, the cell lines B4 and HAB, which are derived from 3460 cells and have approx. 60 and 100% bromodeoxyuridine substitution in DNA, respectively, show an approx. 50-fold higher enzyme activity. Deoxycytidine deaminase activity can be "induced" in 3460 cells by growth in 10(-5) M bromodeoxyuridine, as well as by the other halogenated pyrimidines, iododeoxyuridine and chlorodeoxy-uridine. The time required for maximal enzyme activity to accrue (approx. 8 days) suggests that new genetic expression is required for enhanced deoxycytidine deaminase activity and inhibition of induction in the presence of Ara. C shows that bromodeoxyuridine must be incorporated into DNA. In addition, the extent of enhanced deoxycytidine deaminase activity is directly related to the level of bromodeoxyuridine substitution in DNA. Another hamster cell line, BHK21/C13, which shows no detectable deoxycytidine deaminase activity, cannot be induced by bromodeoxyuridine. These results are discussed with respect to a mechanism by which bromodeoxyuridine may alter gene expression due to an altered binding of both positive and negative regulatory proteins to DNA.
D001973:Bromodeoxyuridine / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D003561:Cytarabine / Q000494:pharmacology; D003844:DCMP Deaminase / Q000096:biosynthesis*; D004247:DNA / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D007065:Idoxuridine / Q000494:pharmacology; D009709:Nucleotide Deaminases / Q000096:biosynthesis*
D003561:Cytarabine; D004247:DNA; D009709:Nucleotide Deaminases; D003844:DCMP Deaminase; D001973:Bromodeoxyuridine; D007065:Idoxuridine