On the roles of calcium ion during potassium induced contracture in the smooth muscle cells of the rabbit main pulmonary artery.
The half decay time of the K-induced contracture of rabbit main pulmonary artery following pretreatment with Ca-free EGTA containing solution was 110 sec. A Ca-free K-solution did not generate contraction while noradrenaline, acetylcholine and prostaglandin F2alpha-containing solution did evoke contracture. The decays of the chemically induced mechanical response in Ca-free solution against the exposure times could be classified into three components (2 min, 28 min and over 100 min, respectively). When the membrane depolarization produced by excess K+ was simulated in Krebs solution by application of current, the generated mechanical response was smaller than that produced by 118 mM K+. When the membrane potential was clamped at the resting level before, during and after application of the excess K+, and excess K+ still evoked contracture. The amplitudes of contracture depended on [K]o. The effects of various [K]o on the length constant of the tissue were also observed in relation to the clamping condition. It is postulated that the mechanical response of the pulmonary artery induced by excess K is mainly due to influx of Ca++ and the depolarization plays only a minor role. This means that release of stored Ca by depolarization is not an essential factor in generation of K-induced contracture in this tissue.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology / Q000502:physiology; D011460:Prostaglandins F / Q000494:pharmacology; D011651:Pulmonary Artery / Q000502:physiology; D011817:Rabbits
D011460:Prostaglandins F; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium; D009638:Norepinephrine