Time-course of actinomycin D inhibition of tobacco mosaic virus multiplication relative to the rate of spread of the infection.
The time-course of inhibition of tobacco mosaic virus synthesis in mechanically inoculated leaves by actinomycin D was not correlated with cell-to-cell spread of the infection, as previously shown to be the case with 2-thiouracil and guanidine inhibition. The amount of inhibition by actinomycin D declined after inoculation much more rapidly than the infection spread. An actinomycin-D-sensitive step or its product occurred in cells before they became infected.
D004279:DNA, Viral / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology*; D010947:Plants, Toxic; D013997:Time Factors; D014026:Nicotiana / Q000378:metabolism; D014027:Tobacco Mosaic Virus / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
D004279:DNA, Viral; D003609:Dactinomycin