Effect of dimethyl sulfate on the secondary structure of DNA.
Hydroxyapatite chromatography has been used to demonstrate that alkylation of DNA at neutral pH may lead to denaturation under conditions where no significant depurination occurs. Presence of salt has a preventive effect on such denaturation.
D000477:Alkylating Agents; D002845:Chromatography; D004247:DNA; D006882:Hydroxyapatites; D008697:Methane / Q000031:analogs & derivatives*; D009691:Nucleic Acid Denaturation; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids
D000477:Alkylating Agents; D006882:Hydroxyapatites; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids; D004247:DNA; C007482:dimethyl sulfate; D008697:Methane