A reappraisal of the Coulter Counter technique for measuring calcium oxalate crystal size.
A modification of the technique for measuring crystal size using a Coulter Counter is described. The method differs from that previously published in that counts are taken at equal size increments rather than at constant threshold settings. This ensures that equal emphasis is placed on all particles, regardless of size, thereby preventing the contribution of the larger particles being artificially magnified in relation to the smaller particles as has previously been the case. A mixture of latex particles of three known sizes is used to demonstrate the improvement in resolution and accuracy afforded by the modified technique.
D003461:Crystallography / Q000295:instrumentation / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D007840:Latex; D008863:Microspheres; D010070:Oxalates / Q000032:analysis; D010316:Particle Size; D014545:Urinary Calculi / Q000652:urine*
D007840:Latex; D010070:Oxalates