An autoradiographic study of satellite cell differentiation into regenerating myotubes following transplantation of muscles in young rats.
Satellite cells were traced autoradiographically during the regeneration of skeletal muscle in young Sprague-Dawley rats. Approximately 31% of the satellite cells in uninjured muscles appeared labelled after three injections of tritiated thymidine; none of the myonuclei were labelled in the same muscles. Four to six days after transplanting the radioactive muscles to non-radioactive littermates, regenerating myotube nuclei in the host appeared labelled. Thus, this study confirms that satellite cells in young rats can differentiate into multinucleated myotubes following muscle injury.
D000818:Animals; D001345:Autoradiography; D002454:Cell Differentiation; D005260:Female; D008297:Male; D008854:Microscopy, Electron; D009132:Muscles / Q000502:physiology / Q000637:transplantation* / Q000648:ultrastructure; D051381:Rats; D012038:Regeneration; D013997:Time Factors; D014184:Transplantation, Homologous