Rapid and sensitive gas chromatographic quantitation of morphine codeine and O6-acetylmorphine in illicit heroin using an electron capture detector.
Morphine, codeine and O6-acetylmorphine are reacted with heptafluorobutyric anhydride, rendering them suitable for electron capture detection and quantitation. The fluorinated derivatives are extracted from an acetonitrile-sodium bicarbonate solution into light petroleum in a rapid one-step extraction procedure. The derivatives are chromatographed on a stationary phase of 3% OV-17 on Gas-Chrom Q. Morphine, codeine and O6-acetylmorphine can be readily quantitated in heroin at levels as low as 0.001%, 0.01% and 0.01% respectively. Reproducibility, linearity and recovery studies are described.
D002849:Chromatography, Gas; D003061:Codeine / Q000032:analysis*; D003932:Heroin / Q000032:analysis*; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis; D009020:Morphine / Q000032:analysis*; D009022:Morphine Derivatives / Q000032:analysis*
D013287:Illicit Drugs; D009022:Morphine Derivatives; D003932:Heroin; D009020:Morphine; D003061:Codeine