[Uptake of acetate by Acinetobacter calcoaceticus].
The uptake of acetate by intact nongrowing cells of Acinetobacter calcoaceticus was studied in dependence on the C-source (acetate, n-alcanes, yeast extract, succinate, L-malate) and the growth phase. Single kinetic parameters of acetate uptake were determined. The best acetate uptake was observed with cells cultivated with acetate as the only C-source. Bacteria in the early growth phase were found to transfer acetate twice as fast as cells of the late logarithmic growth phase. The uptake of acetate can be described by a biphasic saturation kinetics with 2 Km values: the Km value for the first phase being 1.10(-5) M, and for the second one, 1.8 .10(-4) M. The corresponding maximal uptake rates are 8 and 37 mM/min/mg dry weight, respectively. Alpha-ketoglutarate, fumarate, L-malate, and oxalacetate inhibit the initial uptake of acetate. Uranylacetate, inhibitors of the respiratory chain and proton conductors in part completely inhibit the uptake of acetate.
D000085:Acetates / Q000378:metabolism*; D000150:Acinetobacter / Q000254:growth & development / Q000378:metabolism*; D003864:Depression, Chemical; D005650:Fumarates / Q000494:pharmacology; D007656:Ketoglutaric Acids / Q000494:pharmacology; D007700:Kinetics; D008293:Malates / Q000494:pharmacology; D010071:Oxaloacetates / Q000494:pharmacology
D000085:Acetates; D005650:Fumarates; D007656:Ketoglutaric Acids; D008293:Malates; D010071:Oxaloacetates
Aufnahme von Azetat durch Acinetobacter calcoaceticus.