Isolation of a human prostate carcinoma cell line (DU 145).
A long-term tissue culture cell line has been derived from a human prostate adenocarcinoma metastatic to the brain. The cell line, DU 145, has been passaged 90 times in vitro over a period of 2 years. The cells are epithelial, grow in isolated islands on plastic Petri dishes, and form colonies in soft agar suspension culture. Karyotypic analysis demonstrates an aneuploid human karyotype with a modal chromosome number of 64. Distinctive marker chromosomes (a translocation Y chromosome, metacentric minute chromosomes and three large acrocentic chromosomes) have been identified. Electron microscopy of the original tumor tissue and of the tissue culture cell line show a remarkable similarity in cell organelle structure.
D000135:Acid Phosphatase / Q000378:metabolism; D000230:Adenocarcinoma / Q000235:genetics / Q000648:ultrastructure; D000368:Aged; D000782:Aneuploidy; D001921:Brain / Q000648:ultrastructure; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology; D002460:Cell Line; D002869:Chromosome Aberrations; D046508:Culture Techniques; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000235:genetics / Q000648:ultrastructure
D000135:Acid Phosphatase