Immunization of mice against Fusobacterium necrophorum infection by perenteral or oral administration of vaccine.
Immunization of mice against Fusobacterium necrophorum infection was attempted by using 3 vaccination procedures: (1) intraperitoneal (IP) injection of F necrophorum cells in saline solution, (2) IP injection of cells with added aluminum hydroxide adjuvant, and (3) feeding of a powdered mouse diet containing lyophilized cells. One or 2 weekly IP injections of the bacteria cells (in saline solution) for 3, 6, or 12 weeks resulted in protection of 48.7% to 64.5% of the mice against challenge exposure. Of the 2 control groups (given saline solution only), 100% and 97.4% became infected. Weekly IP injections of bacterial cells in an aluminum hydroxide adjuvant for 3, 6, or 12 weeks resulted in protectivity of 54.1% to 77.5%. Of the control mice (given adjuvant only), 97.5% became infected. Bacterial cells fed to mice at a dose level of 1.5 mg (dry weight)/g of powdered diet for 30 days (4 or 5g of diet each day) resulted in only a delay in the mean time of death as compared with the rapid death of the control mice. The feeding dose of 0.15 mg of cells/g of diet did not delay the mean time of death.
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000284:Administration, Oral; D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D005260:Female; D005674:Fusobacterium Infections / Q000517:prevention & control*; D005675:Fusobacterium necrophorum / Q000276:immunology*; D007114:Immunization; D007274:Injections, Intraperitoneal; D051379:Mice
D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D000536:Aluminum Hydroxide