Decay-induced incorporation of tritium into nucleosides in aqueous solutions.
In view of environmental and biological implications, the decay-induced incorporation of tritium from T2 into nucleosides in aqueous solutions has been studied by chromatographic methods. It could be shown that at T2 concentrations of about 1 to 2 mCi/ml, labeling of thymidine or deoxyuridine mainly occurs via the 3HeT+ decay ion rather than via beta--radiolysis. In a saturated solution of thymidine, the number of thymidine molecules labeled per tritium decay (L-value) is only 8 X 10(-4), concomitantly 0.6 HTO molecules are also formed, together with CH3T (L = 0.2) and an unidentified organic product (L = 10(-3). Compared to identical concentrations of HT and HTO in aqueous deoxyuridine solutions, the labeling efficiency of T2 is an order of magnitude higher.
D003857:Deoxyuridine; D007553:Isotope Labeling; D009705:Nucleosides; D013936:Thymidine; D014316:Tritium
D009705:Nucleosides; D014316:Tritium; D013936:Thymidine; D003857:Deoxyuridine