Anomalous migration of leghaemoglobin on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.
The estimate of the molecular weight of leghaemoglobin by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis is about 20% too low. This is due to an anomalously high limiting relative mobility. Leghaemoglobin binds 1.4 g of sodium dodecyl sulphate/g of protein with a concomitant decrease in the helical content from 71-72% to 49-51%.
D004591:Electrophoresis, Polyacrylamide Gel; D006420:Hemeproteins / Q000302:isolation & purification*; D007874:Leghemoglobin / Q000302:isolation & purification*; D008970:Molecular Weight; D012967:Sodium Dodecyl Sulfate
D006420:Hemeproteins; D007874:Leghemoglobin; D012967:Sodium Dodecyl Sulfate