Effects of experimental diabetes and food intake on rat intestine and serum alkaline phosphatase.
Alkaline phosphatase activity of rat serum was reduced 50% by fasting the animal for 24 hours. Diabetes, induced by alloxan or streptozotocin, increased serum alkaline phosphatase 3- to 5-fold in fed rats and the elevated activity was reduced by insulin administration. In the absence of insulin, fasting alone was able to reduce the serum alkaline phosphatase of diabetic rats to control values. The elevated serum isozyme was found to be of intestinal origin by the use of appropriate inhibitors and electrophoretic mobility following neuraminidase treatment. It is concluded that food intake, particularly the hyperphagia of diabetes, plays a major role in regulating the concentration of intestine and serum alkaline phosphatase in the rat.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001842:Bone and Bones / Q000201:enzymology; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004032:Diet; D005215:Fasting; D007328:Insulin / Q000494:pharmacology; D007421:Intestine, Small / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D009747:Nutritional Physiological Phenomena; D051381:Rats
D007328:Insulin; D007527:Isoenzymes; D000469:Alkaline Phosphatase