A new method of carboxyhaemoglobin determination.
A quick and accurate method of determining the concentration of carboxyhaemoglobin (COHb) in blood has been developed. The method uses a dual wavelength double beam spectrophotometer in the 1st derivative mode, linked to a digital voltmeter (DVM), with the two beams set 3 nm apart around an isobestic point of reduced haemoglobin (Hbred) and carboxyhaemoglobin at 579 nm. The 1st derivative mode measures the slope, and this slope is proportional to the concentration of COHb.
D000818:Animals; D002263:Carboxyhemoglobin / Q000032:analysis*; D004285:Dogs; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D051381:Rats; D013053:Spectrophotometry / Q000379:methods
D006454:Hemoglobins; D002263:Carboxyhemoglobin