Inhibition of haemopoietic stem cell proliferation by a diffusible product of bone marrow cells.
In a double diffusion chamber (DC) culture system bone marrow cells elaborated diffusible factor(s) that prevented spleen colony-forming cells (CFU-S), but not PHA stimulated lymphocytes, from entering cell cycle. Mature granulocytes and macrophages did not produce such factors(s). Various number of steady-state or regenerating mouse bone marrow cells were cultured in single diffusion chamber for periods up to 7 d. After the initial cell loss,the net growth of CFU-S was inversely related to both total cell number and CFU-S number in DC. Diffusible factors rather than cell-to-cell contact appeared to be involved in the inhibition, even though we were not able to demonstrate inhibition of net growth of CFU-S with the double chamber approach.
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D002999:Clone Cells; D005260:Female; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology