Gas chromatography chemical ionization mass spectrometric analysis of diminazene in plasma.
A quantitative and sensitive method was developed  for the determination of diminazene in plasma. The assay involves the reduction of diminazene to 4-aminobenzamidine and 4-hydrazinobenzamidine. The latter is further reduced to give an additional mole of 4-aminobenzamidine which is extracted, acetylated and condensed with hexafluoroacetylacetone to form a volatile derivative that is subsequently analyzed by gas chromatography chemical ionization mass spectrometry. 4-Aminobenzylamidine was synthesized and used as an internal standard. The method is reproducible and its sensitivity limit using 1 ml of plasma is 0.1 microgram diminazene ml-1. This sensitivity limit is sufficient to detect plasma levels in cattle following therapeutic doses of the drug.
D000578:Amidines / Q000097:blood*; D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas / Q000379:methods; D004133:Diminazene / Q000097:blood* / Q000627:therapeutic use; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods; D013997:Time Factors
D000578:Amidines; D007477:Ions; D004133:Diminazene