[Adaptation on the ABA-100 of the optimized method of measurement of serum aspartate aminotransferase activity].
The analytical modifications necessary for adaptation of the SFBC technic for measurement of the activity of TGO method and those obtained by the technic adapted on ABA 100 suggest that the modifications proposed have no influence on the result of the enzyme estimation. The activation of TGO by addition of its coenzyme was assessed using mathematical models uniting the values of TGO recorded with and without pyridoxal phosphate. It has been shown that the parabolic model is more suitable than the linear model to explain the relation between transaminase activity determined without pyridoxal phosphate and that measured in the presence of the coenzyme. Using estimations carried out in 850 blood donors, it was possible to show that the reference interval of TGO lay between 7 and 28 mUI/ml. In man, the value of TGO is statistically higher than in women. On the other hand between 20 and 60 years there does not seem to be any significant difference depending on age.
D000328:Adult; D000367:Age Factors; D000469:Alkaline Phosphatase / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000032:analysis / Q000097:blood*; D001322:Autoanalysis / Q000379:methods*; D004347:Drug Interactions; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008875:Middle Aged; D011732:Pyridoxal Phosphate; D012016:Reference Values; D012737:Sex Factors
D007202:Indicators and Reagents; D011732:Pyridoxal Phosphate; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase
Adaptation sur ABA-100 de la méthode optimisée de mesure de l'activité sérique de l'aspartate aminotransférase.