[Photochemiluminescence of tryptophan-containing peptides and proteins during photooxidation. VII. Mechanism of sensitization by eosin of the photochemiluminescence of human serum albumin solutions].
Dependence of photochemoluminescence (PCL) intensity of serum albumin solutions with eosine initiated under light illumination with the wave length of 546 nm on oxygen concentration in solution has been studied. The luminescence intensity was shown to increase gradually with the increase of oxygen concentration. The relationship obtained is described by an empyric formula. Experimental data on the change of PCL intensity dependent on protein and dye concentrations have been obtained. A three-dimensional diagram of such relationship is plotted: this surface has a complex profile. An uncomplicated relationship between PCL intensity and protein concentration was analysed on the strength of the data obtained. The experimental, rated and literature data allowed a quantitative description of sensitized PCL of serum albumin solutions with eosine.
D005452:Fluoresceins; D006801:Humans; D066298:In Vitro Techniques; D049449:Luminescence; D012709:Serum Albumin
D005452:Fluoresceins; D012709:Serum Albumin
Fotokhemiliuminestsentsiia triptofansoderzhashchikh peptidov i belkov pri fotookislenii. VII. O mekhanizme sensibilizirovannoĭ éozinom fotokhemiliuminestsentsii rastvorov syvorotochnogo al'bumina cheloveka.