Perfusion preservation of hearts for 6 to 9 days at room temperature.
The combination of a defined medium with single-pass perfusion has made possible long-term maintenance of beating rat hearts at 22 degrees C in vitro. The 6- to 9-day survival period appears to be the longest so far reported for hearts. This method provides a stable system which should be useful for investigating the role of single factors in myocardial preservation and evaluating the effects of exposure to pharmacological and toxicological agents.
D000818:Animals; D003470:Culture Media; D006321:Heart; D009200:Myocardial Contraction; D009206:Myocardium / Q000473:pathology; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion; D051381:Rats; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
D003470:Culture Media