A simple and reproducible method to evaluate granulocyte adherence.
A simple method was devised to measure granulocyte adherence in whole blood. Columns of glass beads (4.5 mm diameter) in disposable plastic syringes were used. The assay showed great reproducibility when done in triplicate, the day to day variations in a given individual being minimal. Previous incubation of the blood with different ethanol concentrations diminished granulocyte adherence. The assay is easy to perform and does not require special equipment.
D002448:Cell Adhesion / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D005898:Glass; D006098:Granulocytes; D006801:Humans; D007962:Leukocytes
D000431:Ethanol