Fluorescence energy transfer between epsilon-ATP at the nucleotide binding site and N-(4-dimethylamino-3,5-dinitrophenyl)-maleimide at Cys-373 of G-actin.
The method of fluorescence energy transfer is used to measure the distance from the nucleotide binding site to Cys-373 of G-actin. The fluorescent ATP analogue 1-N6-ethenoadenosine 5'-triphosphate was used as donor and N-(4-dimethylamino-3,5-dinitrophenyl)-maleimide was used as acceptor. From the measurements of the efficiency of fluorescence energy transfer by both static and time resolved fluorometries, the distance between nucleotide binding site and Cys-373 residue of G-actin was calculated to be about 30 A.
D000199:Actins; D000595:Amino Acid Sequence; D001665:Binding Sites; D003545:Cysteine; D004735:Energy Transfer; D004985:Ethenoadenosine Triphosphate; D008301:Maleimides; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence
D000199:Actins; D004985:Ethenoadenosine Triphosphate; D008301:Maleimides; D003545:Cysteine