A radioimmunoassay for 1,25-dihydroxycholecalciferol.
1. 1alpha,25-Dihydroxy-25-hemisuccinate cholecalciferol has been synthesized and conjugated to bovine serum albumin. 2. This conjugate is immunogenic; when injected into rabbits antibodies of high affinity for 1alpha,25-dihydroxycholecalciferol were obtained. 3. Vitamin D metabolites lacking the 1alpha-hydroxy group were of lower cross-reactivity with the antibodies. 4. By using these antibodies and 1alpha,25-[23,24-3H]dihydroxycholecalciferol as tracer a sensitive radioimmunoassay has been developed capable of detecting 20 pg of 1alpha,25-dihydroxycholecalciferol.
D000818:Animals; D000917:Antibody Formation; D003429:Cross Reactions; D004100:Dihydroxycholecalciferols / Q000032:analysis*; D006887:Hydroxycholecalciferols / Q000032:analysis*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D012710:Serum Albumin, Bovine
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D012710:Serum Albumin, Bovine