Synthesis and metabolism of 5beta-[11,12-3H]cholestane-3alpha, 7alpha-diol.
5beta-[11,12-3H]Cholestane-3alpha,7alpha-diol was synthesized as follows. 5beta-Cholestane-3alpha,7alpha,12atriol 3,7-diacetate was treated with phosphorus oxychloride in pyridine solution and then the product, 5beta-cholest-11-ene-3alpha,7alpha-diol diacetate, was hydrogenated in acetic acid solution using platinum oxide as a catalyst under an atmosphere of tritium gas. 5beta-[11,12-3H]Cholestane-3alpha,7alpha-diol thus obtained was readily hydroxylated at C-26 by mitochondria in the presence of isocitric acid, magnesium chloride and potassium cyanide.
D000818:Animals; D002777:Cholestanols / Q000138:chemical synthesis* / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008722:Methods; D051381:Rats; D013250:Steroid Hydroxylases / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
D002777:Cholestanols; D013250:Steroid Hydroxylases