Temperature sensitivity of the erythrocyte membrane potential as determined by cyanine dye fluorescence.
We have used the cyanine dye fluorescence technique to measure the membrane potential of human erythrocytes as a function of temperature. With erythrocytes starved of glucose, there is an abrupt decrease in membrane potential centered at 38 degrees which is reversible up to 41 degrees, and irreversible at higher temperatures. With erythrocytes supplemented with glucose, the thermally induced transition is centered at 41 degrees and is reversible up to the highest temperature measured, 45 degrees. These results extend previous spectroscopic studies with erythrocyte membranes which demonstrated a thermally induced transition in protein tertiary or quaternary structure that is irreversible above 42 degrees.
D004058:Diffusion; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology*; D004912:Erythrocytes / Q000378:metabolism / Q000502:physiology*; D005453:Fluorescence; D005456:Fluorescent Dyes; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D013696:Temperature; D014634:Valinomycin / Q000494:pharmacology
D005456:Fluorescent Dyes; D008565:Membrane Proteins; D014634:Valinomycin; D005947:Glucose; D011188:Potassium