Influence of hexobendine, dipyridamole, dilazep, lidoflazine, inosine and purine riboside on adenosine uptake by the isolated epithelium of guinea pig jejunum.
The uptake of (14C)adenosine by isolated epithelium of guinea pig jejunum, administered on the blood side, was inhibited by hexobendine, dipyridamole, dilazep and lidoflazine. On the lumen side, however, weak inhibition was observed with lidoflazine only and no significant change was recorded with hexobendine, dipyridamole or dilazep. This difference was not altered when the degradation of hexobendine by the jejunal epithelium was blocked by physostigmine. When adenosine uptake was already reduced by purine riboside, further addition of hexobendine, dipyridamole, dilazep or lidoflazine caused divergent changes depending on the side of administration. Adenosine uptake was further diminished on the blood side, but raised towards control values on the lumen side. By contrast, inosine inhibited adenosine uptake on both sides of the epithleium. The results suggest that the mechanism of adenosine uptake is different on either side with respect to inhibition characteristics, corresponding to differences in morphology and function of the two sides of the intestinal epithelium.
D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D004109:Dilazep / Q000494:pharmacology; D004176:Dipyridamole / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism; D006168:Guinea Pigs; D006592:Hexobendine / Q000494:pharmacology; D007288:Inosine / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects; D007583:Jejunum / Q000378:metabolism*; D008013:Lidoflazine / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology*
D010879:Piperazines; D011687:Purines; D014665:Vasodilator Agents; D007288:Inosine; D004176:Dipyridamole; D006592:Hexobendine; D004109:Dilazep; D008013:Lidoflazine; D000241:Adenosine