Affinity chromatography of proteases of hydroxyalkyl methacrylate gels with covalently attached inhibitors.
The efficient isolation of trypsin and chymotrypsin from a crude pancreatic extract was achieved by affinity chromatography on specific adsorbents prepared by coupling of both naturally occurring protease inhibitors and also synthetic low-molecular-weight protease inhibitors to hydroxyalkyl methacrylate gels. Specific sorbents prepared with synthetic inhibitors are stable and are suitable for the isolation of chymotrypsin and trypsin even on a large scale.
D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000302:isolation & purification*; D008689:Methacrylates; D010179:Pancreas / Q000201:enzymology; D014357:Trypsin / Q000302:isolation & purification*
D008689:Methacrylates; D002918:Chymotrypsin; D014357:Trypsin