Ram sperm mitochondrial DNA.
Circular DNA was isolated from mitochondrial fractions of ram spermatozoa by SDS treatment followed by convex sucrose gradient centrifugation. The DNA had a contour length of 5.0 micron. Its buoyant density was 1.6983 g cm-3, which was smaller than two nuclear DNA components with buoyant densities of 1.6999 and 1.7156 g cm-3, found in ram spermatozoa. The Tm of the mitochondrial DNA was 69.7 degrees C. The mole fraction G+C calculated from the buoyant density and melting temperature was 39.1% and 38.6%, respectively.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004270:DNA, Circular; D004272:DNA, Mitochondrial; D008297:Male; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012756:Sheep / Q000502:physiology*; D013094:Spermatozoa / Q000032:analysis*
D004270:DNA, Circular; D004272:DNA, Mitochondrial