Biological properties and physical map of the genome of a new papovavirus, HD virus.
The superhelical DNA of the HD papovavirus is heterogeneous and consists of two discrete size classes with molecular weights of 3.45 X 10(6) and 3.25 X 10(6). Both size classes of DNA are encapsidated into HD virion particles. Their relative intracellular amounts differ, depending on the cell system. Vero-76 carrier cultures in which HD virus was detected contain both size classes of DNA, with the larger molecules prevailing by a factor of 10. Five clonal lines derived from Vero-76 cell cultures contain exclusively the larger DNA. On the other hand, after cocultivation of Vero-76 with CV-1 cells for several passages, minicircular DNA is accumulated such that both size classes are synthesized in equal amounts. Any of the originally viral DNA-producing cell lines may, upon subcultivation, cease yielding virus. The RITA cell line of Cercopithecus aethiops origin is the only cell line among numerous ones tested which upon infection permits the establishment of a one-step growth cycle. However, between 6 and 8 days after infection, viral DNA synthesis is discontinued, and a persistent viral infection cannot be established. Physical maps of the genomes were constructed, and it could be shown that the smaller, minicircular DNA had originated from the larger DNA as the result of a deletion. The sequences missing in the minicircular DNA are confined to the relative map position 0.15 to 0.21.
D002460:Cell Line; D004261:DNA Replication; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004278:DNA, Superhelical / Q000032:analysis / Q000096:biosynthesis; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D005814:Genes, Viral; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D027383:Papillomaviridae / Q000032:analysis* / Q000378:metabolism; D027382:Polyomaviridae
D004278:DNA, Superhelical; D004279:DNA, Viral; D004262:DNA Restriction Enzymes