Studies on the properties of the singlet oxygen-like factor produced during lipid peroxidation.
The singlet oxygen reaction product of various trapping agents is observed during enzymic and nonenzymic peroxidation of microsomes as well as during the peroxidation of pure lipids extracted from microsomes. We now wish to report that purified fatty acid hydroperoxide alone, as well as peroxidized microsomal lipid and cumene hydroperoxide also form the singlet oxygen reaction product with 2,5-diphenylfuran. The reaction product (cis-1,2-dibenzoylethylene) was observed to be formed in an anaerobic system, with or without EDTA. The data indicate that a reaction of hydroxyl radicals with 2,5-diphenylfuran cannot account for the formation of dibenzoylethylene in these systems. These results are consistent with a hypothesis that the singlet oxygen-like factor was formed from the lipid peroxides per se and, in addition, supports the possibility that either the peroxides can react directly with diphenylfuran to produce dibenzoylethylene or that the self-reaction of organic peroxides may form an intermediate product which can react directly with singlet oxygen-trapping agents to produce substances which are identical to a reaction of the trapping agents with singlets oxygen.
D000818:Animals; D008042:Linolenic Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen; D010545:Peroxides / Q000378:metabolism*; D010655:Phenylenediamines / Q000494:pharmacology; D051381:Rats
D008042:Linolenic Acids; D010545:Peroxides; D010655:Phenylenediamines; D010100:Oxygen