[Solid-phase enzyme immunoassay for specific immunoglobulin G and immunoglobulin M antibodies of rubella virus (author's transl)].
A sensitive solid-phase enzyme-immunoassay for the detection of rubella virus specific IgG and IgM antibodies has been developed. Wells of polystyrene trays were sensitized with purified rubella antigen. A substrate employed in enzyme-histochemistry, which gives a stable staining, was used to allow direct determination of titre values. One hundred and thirty-five human sera were tested. Antibodies titres, detected using this glucose oxidase immunoassay, correlated well with those obtained using a virus haemagglutination inhibition test. With this procedure sera can be tested without any pretreatment and evaluated within one day. Moreover, TIE is more sensitive and specific antibodies are detected earlier.
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D002648:Child; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D012409:Rubella / Q000175:diagnosis*; D012412:Rubella virus / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Titrage immunoenzymatique des anticorps IgG et IgM spécifiques de la rubéole.