Quantitative verification of the existence of high molecular weight protein aggregates in the intact normal human lens by light-scattering spectroscopy.
The method of quasi-elastic light-scattering spectroscopy was used to establish quantitatively the concentration of high mmolecular weight (HMW) aggregates present in the normal human intact lens as a function of age. The concentration of HMW proteins increases monotonically with age. HMW proteins are absent in the infant lens, but represent 3% of the total soluble lens protein at age 60 years. The percent concentration of HMW proteins measured in intact lenses of various ages by quasi-elastic light scattering is in striking agreement with values determined biochemically.
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000818:Animals; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D003459:Crystallins / Q000032:analysis*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007908:Lens, Crystalline / Q000032:analysis*; D008027:Light; D008875:Middle Aged; D008970:Molecular Weight; D011817:Rabbits; D012542:Scattering, Radiation; D013057:Spectrum Analysis
D003459:Crystallins