Stability of mutations conferring temperature sensitivity on tobacco mosaic virus.
Among several temperature-sensitive mutants of tobacco mosaic virus, reversion to wild-type phenotype is an event sufficiently rare that essentially pure stocks of mutant virus can easily be maintained. The temperature-sensitive phenotype of many of these mutants is stringently expressed. Our data show that most of our mutants are suitable for use in biochemical experiments.
D009154:Mutation; D010641:Phenotype; D013696:Temperature; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000254:growth & development; D014779:Virus Replication