Altered phospholipids in human rheumatoid synoviocytes.
A specific cytochemical reaction for freely available phospholipids has shown a raised concentration of such phospholipids in the lining cells of human synovial membranes removed from rheumatoid joints. Quantitative measurement, by microdensitometry, of the amount of reaction-product per cell showed that the rheumatoid synoviocytes contained almost three times the amount of free phospholipids present in the equivalent nonrheumatoid cells; statistically the difference was highly significant. Evidence from studies in which the bound phospholipids were 'unmasked' by methanol-chloroform confirmed the view that the increased content of freely available phospholipids was related to altered lipid-protein binding rather than to an increase in total phospholipids.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000032:analysis*; D011485:Protein Binding; D013583:Synovial Membrane / Q000032:analysis* / Q000166:cytology
D010743:Phospholipids