Affinity chromatography of serum proteins using immobilized lectin from Vicia faba.
When human serum is applied to a column of Sepharose-insolubilized lectin from Vicia faba, some serum proteins are bound which can be eluted by means of 0.1 M glucose solution. These proteins are parts of the immunoglobulins IgA, IgG, IgM, and the alpha2-macroglobulin. These particular types of serum protein are bound specifically, due perhaps to some structural variation in the carbohydrate moieties they contain.
D001798:Blood Proteins / Q000276:immunology*; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D037102:Lectins; D037121:Plant Lectins; D010946:Plants, Medicinal; D011485:Protein Binding; D012639:Seeds
D001798:Blood Proteins; D007136:Immunoglobulins; D037102:Lectins; D037121:Plant Lectins