Removal of unbound sodium dodecyl sulfate (SDS) from proteins in solution by electrophoresis through triton x-100-agarose.
Residual sodium dodecyl sulfate (SDS) introduces artifacts into immuno- and counterimmunoelectrophoretic analysis of proteins which have been eluted from preparative SDS-polyacrylamide gels. Unbound SDS can be removed by electrophoretic passage of eluted solutions through a barrier of Triton X-100 in agarose in which the anionic and non-ionic detergents interact to form micelles.
D003378:Counterimmunoelectrophoresis; D004912:Erythrocytes; D006023:Glycoproteins / Q000032:analysis; D007122:Immunoelectrophoresis; D011092:Polyethylene Glycols; D011506:Proteins / Q000032:analysis*; D012685:Sepharose; D012967:Sodium Dodecyl Sulfate / Q000302:isolation & purification*
D006023:Glycoproteins; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D012685:Sepharose