Complement receptors: specific detection by molecular complexes.
Immune complexes of BSA-anti BSA (IgG F(ab')2) will activate human complement by the alternate pathway. The C3 bound to these complexes binds to human peripheral blood mononuclear cells which bear complement receptors. This interaction is visualized by fluoresceinated antigen (BSA-FITC) or fluorescein conjugated antisera directed at the C3 component of complement. The assay appears to be more sensitive than the usual IgMEAC rosette technique and correlates well with the rosette method.
D000818:Animals; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D001665:Binding Sites; D002417:Cattle; D003176:Complement C3; D003181:Complement C4; D003168:Complement Fixation Tests; D003165:Complement System Proteins; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D012710:Serum Albumin, Bovine / Q000276:immunology
D000936:Antigen-Antibody Complex; D003176:Complement C3; D003181:Complement C4; D007140:Immunoglobulin Fab Fragments; D012710:Serum Albumin, Bovine; D003165:Complement System Proteins