alpha-Amanithin-resistant mutants of Chinese hamster ovary (CHO) cells.
Spontaneous and EMS-induced alpha-amanitin-resistant CHO cells have been isolated and characterized. DNA-dependent RNA polymerase II in cell-free extracts from a mutant (ARM-1) was partially resistant to alpha-amanitin. Growing mutants for several generations in the presence or absence of alpha-amanitin did not change the pattern of inhibition. The mutants grew with a lag following transfer to medium with or without alpha-amanitin. The mutants have an altered RNA polymerase II, and possibly an altered cell membrane.
D000546:Amanitins / Q000494:pharmacology*; D002460:Cell Line; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D009154:Mutation; D010641:Phenotype; D012319:RNA Polymerase II / Q000378:metabolism*
D000546:Amanitins; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases