Quantitation of messenger RNA levels for rat liver 6-phosphogluconate dehydrogenase.
Liver poly(A)-containing RNA isolated from rats in different dietary states was translated in a cell free protein synthesizing system employing reticulocyte lysates. Immunoprecipitation of the cell-free reaction products with goat anti-6-phosphogluconate dehydrogenase followed by sodium dodecyl sulfate-urea-gel electrophoresis showed that the induction of this lipogenic enzyme was accompanied by a corresponding increase in the concentration of its specific translatable mRNA.
D000818:Animals; D002474:Cell-Free System; D004790:Enzyme Induction; D008099:Liver / Q000201:enzymology*; D008297:Male; D010446:Peptide Fragments; D010734:Phosphogluconate Dehydrogenase / Q000096:biosynthesis*; D011061:Poly A / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D013217:Starvation / Q000378:metabolism
D010446:Peptide Fragments; D012333:RNA, Messenger; D011061:Poly A; D010734:Phosphogluconate Dehydrogenase