Radioimmunoassays for androsterone, 5alpha-androstane-3alpha, 17beta-diol and 5alpha-androstane-3beta, 17beta-diol.
Androsterone (3alpha-hydroxy-5alpha-androstan-17-one), 5alpha-androstane-3alpha, 17beta-diol and 5alpha-androstane-3beta, 17beta-diol were conjugated at C-16 through sulfur to bovine and human serum albumin. Rabbits injected with these conjugates produced antibodies suitable for radioimmunoassays of these hormone metabolites. Samples were purified on Sephadex LH-20 columns. Levels of these steroids were measured in a rat blood serum pool and in ovarian tissue extract pools.
D015113:Androstane-3,17-diol / Q000032:analysis* / Q000097:blood; D000731:Androstanes / Q000032:analysis*; D000738:Androsterone / Q000032:analysis* / Q000097:blood; D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D005260:Female; D008968:Molecular Conformation; D010053:Ovary / Q000032:analysis; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D013237:Stereoisomerism; D014020:Tissue Extracts / Q000032:analysis
D000731:Androstanes; D014020:Tissue Extracts; D015113:Androstane-3,17-diol; D000738:Androsterone