Effect of some glycolytic intermediates and palmitoyl-CoA on alpha-glycerophosphate dehydrogenase in mitochondria isolated from liver of triiodothyronine-treated rats.
alpha-Glycerophosphate dehydrogenase (EC 1.1.99.5) in mitochondria from liver of the triiodothyronine-treated rats is competitively inhibited by phosphoenolpyruvate, glyceraldehyde 3-phosphate and 3-phosphoglycerate, the apparent Ki values for phosphoenolpyruvate being 0.76 mM at pH 7.0, 1.7 mM at pH 7.4 and 3.5 mM at pH 7.7. The apparent Ki values for glyceraldehyde 3-phosphate and 3-phosphoglycerate are also pH-dependent. Other glycolytic intermediates, such as 2-phosphoglycerate, 2,3-diphosphoglycerate, pyruvate, glucose 6-phosphate, fructose 6-phosphate and fructose 1,6-diphosphate did not alter significantly alpha-glycerophosphate dehydrogenase activity. Palmitoyl-CoA is a competitive inhibitor of this enzyme, with Ki value of about 30 micron.
D000214:Acyl Coenzyme A / Q000494:pharmacology*; D000818:Animals; D005985:Glyceraldehyde / Q000031:analogs & derivatives / Q000494:pharmacology; D005988:Glyceric Acids / Q000494:pharmacology; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006980:Hyperthyroidism / Q000139:chemically induced* / Q000201:enzymology; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology*; D010728:Phosphoenolpyruvate / Q000494:pharmacology; D051381:Rats; D014284:Triiodothyronine
D000214:Acyl Coenzyme A; D005988:Glyceric Acids; D009943:Organophosphorus Compounds; D014284:Triiodothyronine; D010171:Palmitoyl Coenzyme A; D005985:Glyceraldehyde; D010728:Phosphoenolpyruvate; C005156:3-phosphoglycerate; D005993:Glycerolphosphate Dehydrogenase