In vitro translation of the Two RNAs of Nodamura virus, a novel mammalian virus with a divided genome.
Nodamura virus is a small ribovirus containing two RNA molecules. Both RNAs were found to be active messengers for protein synthesis in cell-free extracts prepared from wheat embryo or HeLa cells. RNA 2 directed synthesis of a 43,000-dalton product, p43, whose tryptic fingerprint was similar to that of the major viral coat protein, vp40 (molecular weight, 40,000). Though p43 appears to be a precursor of vp40, processing did not occur in the cell-free extracts. RNA 1 directed synthesis of a 105,000-dalton protein, p105. Its tryptic fingerprint revealed no evidence of coat protein sequences. Hence, the two RNAs represent genes with few, if any, redundant coding sequences.
D000596:Amino Acids / Q000032:analysis; D002474:Cell-Free System; D006367:HeLa Cells; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D010849:Picornaviridae / Q000032:analysis / Q000378:metabolism*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism*; D014908:Triticum; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D000596:Amino Acids; D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins