[Isolation and characteristics of DNA fragments bound to mitochondrial membrane proteins].
Fragments of rat liver mitochondrial DNA were isolated. In vivo these fragments were able to form the complexes with the proteins of inner mitochondrial membrane. The fragments represent unique DNA regions with the secondary structure, their A-T content being equal to 82%. With the aid of phosphomonoesterase, polynucleotidkinase and gamma-(32P)-ATP mtDNA fragments were labeled and analyzed for oligopyrimidine composition. It was shown that they were enriched in di- and tri-oligo-pyrimidine blocks. The fragments are shown to form in vitro a complex with the membrane proteins. A single protein m. wt. 40,000) was reisolated from the complex.
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D004272:DNA, Mitochondrial / Q000302:isolation & purification; D008565:Membrane Proteins / Q000302:isolation & purification; D008928:Mitochondria / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009695:Nucleic Acid Renaturation; D009711:Nucleotides / Q000032:analysis; D011485:Protein Binding; D051381:Rats
D004272:DNA, Mitochondrial; D008565:Membrane Proteins; D009711:Nucleotides
Vydelenie i kharakteristika fragmentov DNK, sviazannykh s belkami mitokhondrial'nykh membran.