The effect of 1,1,3-tricyano-2-amino-1-propene (TCAP) on RNA synthesis in chick embryo sciatic nerves.
Injections of 1,1,3-tricyano-2-amino-1-propene (TCAP) into chick embryos increases the synthesis of the myelin enzyme 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP). Whether this increase is associated with altered RNA synthesis was investigated by examining pulse 3H-uridine labelled RNA from in ovo and in vitro TCAP treated sciatic nerves. Characterization of the resultant TCAP-RNAs by polyacrylamide gel electrophoresis revealed that they met several criteria of messenger-RNA.
D000588:Amines / Q000494:pharmacology; D000818:Animals; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D009570:Nitriles / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis*; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D000588:Amines; D009570:Nitriles; D012313:RNA