Serum protein depletion by cultured rat embryos (1).
Cultures of 10-day rat embryos depleted a protein band with a molecular weight of approximately 125,000 from the rat serum medium. Delayed centrifuged serum differed from immediately centrifuged serum by a reduction in the staining intensity of the 125,000 molecular weight protein band and by the absence of two high molecular weight (greater than 200,000) protein bands.
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002498:Centrifugation; D003470:Culture Media; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008970:Molecular Weight; D051381:Rats
D001798:Blood Proteins; D003470:Culture Media