Decreased heparin sensitivity of cycholhexanedione-modified thrombin.
Modification of 5--6 arginine residues of thrombin with 1,2-cyclohexanedione has resulted in the selective abolition of the heparin sensitivity of the enzyme's reaction with antithrombin-III, whereas the antithrombin-III sensitivity of native and modified thrombin was indistinguishable. It is suggested that heparin accelerates the thrombin antithrombin-III reaction by interacting with thrombin.
D000818:Animals; D000991:Antithrombins; D001120:Arginine; D002417:Cattle; D003510:Cyclohexanes; D003512:Cyclohexanones; D006493:Heparin; D006801:Humans; D007700:Kinetics; D013917:Thrombin / Q000502:physiology
D000991:Antithrombins; D003510:Cyclohexanes; D003512:Cyclohexanones; D006493:Heparin; D001120:Arginine; D013917:Thrombin