Activation of the carcinogen N-hydroxy-2-acetylaminofluorene by rat mammary peroxidase.
A peroxidase preparation from rat mammary gland parenchymal cells, a target tissue of arylamine carcinogens, was shown to activate the carcinogen N-hydroxy-2-acetylaminofluorene via a nitroxyl free radical intermediate to the more active carcinogens nitrosofluorene and N-acetoxy-2-acetylaminofluorene. Hydrogen peroxide, cumene hydroperoxide, and linoleic acid hydroperoxide were effective as substrates. The antioxidants ascorbate, propyl gallate and reduced glutathione prevented the free radical activation route.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002273:Carcinogens; D003497:Cyclic N-Oxides; D005260:Female; D005449:Fluorenes; D005609:Free Radicals; D006881:Hydroxyacetylaminofluorene; D008321:Mammary Glands, Animal / Q000201:enzymology*; D051379:Mice; D010544:Peroxidases / Q000378:metabolism*
D000975:Antioxidants; D002273:Carcinogens; D003497:Cyclic N-Oxides; D005449:Fluorenes; D005609:Free Radicals; D006881:Hydroxyacetylaminofluorene; D010544:Peroxidases