Supercooled water as medium for enzyme reactions at subzero temperatures.
A water-in-oil emulsion technique was employed to investigate enzyme-catalyzed reactions at sub-zero temperatures in the supercooled liquid state. The results obtained with a monooxygenase (bacterial cytochrome P-450) clearly indicate the potentialities as well as the technical problems of the procedure which might be successfully used to investigate enzyme systems sensitive to cosolvents.
D003080:Cold Temperature; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004655:Emulsions; D007700:Kinetics; D008722:Methods; D013053:Spectrophotometry; D013816:Thermodynamics
D004655:Emulsions; D003577:Cytochrome P-450 Enzyme System