High-performance liquid-chromatographic determination theophylline in plasma.
A high-performance liquid-chromatographic method is reported for monitoring theophylline in plasma. Samples are deproteinized with 2.5 volumes of acetonitrile and the supernates chromatographed on a reversed-phase column. Absorption at 275 nm is monitored. One can accurately measure 1.5 mg of theophylline per liter, in as little as 10 microliter of plasma, and only about 7 min is required per sample. No interference was found in plasma samples from asthmatic patients. This method is particularly useful for routine therapeutic monitoring of both pediatric and adult patients.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D013806:Theophylline / Q000097:blood*
D013806:Theophylline