Alpha and beta adrenergic effects on human atrial specialized conducting fibers.
We determined the effects of epinephrine on automaticity and action potential characteristics of right atrial specialized fibers (RAF) from human atria obtained during cardiac surgery. RAF were studied with standard microelectrode techniques during superfusion with Tyrode's solution at 37 degrees C. A biphasic response to epinephrine was seen, rate slowing at low agonist concentrations and increasing at high concentrations. The epinephrine-induced slowing of spontaneous rate was due to a decrease in the slope of phase 4 depolarization. At the high epinephrine concentrations RAF hyperpolarized. The alpha-adrenergic blocker, phentolamine, shifted the dose-response curve upward and to the left and enhanced the hyperpolarization of RAF. The beta blocker, propranolol, shifted the curve to the right and decreased the degree of hyperpolarization. Our study suggests the presence of alpha and beta receptors in RAF. The alpha response consists of a slowing of rate, the beta response of an acceleration of rate and hyperpolarization of RAF.
D000200:Action Potentials; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology*; D006325:Heart Atria / Q000187:drug effects*; D006331:Heart Diseases / Q000601:surgery; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D008875:Middle Aged; D010646:Phentolamine / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology
D011433:Propranolol; D004837:Epinephrine; D010646:Phentolamine