Microbiological titration of proteins and of single amino acid content in biological materials without purification and hydrolysis.
A method is described for the microbiological determination of the protein content of biological materials. This method can also be adopted to titrate the concentration of a single amino acid in the protein and has the following advantages: (1) titration can be done without purification and hydrolysis of proteins; (2) the titration graph is a straight line between 25 and 800 microgram/ml; (3) protein values agree with those obtained using the Kjeldhal method; and (4) each mutant requiring one amino acid may be used to titrate the concentration of a single amino acid of the protein. The leucine content of various kinds of flour was measured with this system.
D000596:Amino Acids / Q000032:analysis*; D001407:Bacillus / Q000254:growth & development*; D001681:Biological Assay; D007930:Leucine / Q000032:analysis / Q000378:metabolism; D009154:Mutation; D011506:Proteins / Q000032:analysis* / Q000378:metabolism
D000596:Amino Acids; D011506:Proteins; D007930:Leucine