Characterization of immunoreactive angiotensin in canine cerebrospinal fluid as Des-Asp1-angiotensin II.
1. Immunoreactive angiotensin II was measured in cerebrospinal fluid of four normal dogs. 2. The migration of this immunoreactive angiotensin II on polyacrylamide-slab gel electrophoresis was identical with the migration of the heptapeptide, Des-Asp1-angiotensin II, in each case. 3. The biological activity of the material from canine cerebrospinal fluid in a pressor bioassay was similar to that of Des-Asp1-angiotensin II. 4. The pressor activity of the canine material was abolished by treating the pressor bioassay rat with a competitive antagonistic analogue, Sar1-Ala8-angiotensin II. 5. The results suggest that the biologically active immunoreactive angiotension II present in normal canine cerebrospinal fluid is composed mainly of the heptapeptide fragment of angiotensin II, Des-Asp1-angiotensin II.
D000804:Angiotensin II / Q000031:analogs & derivatives*; D000805:Angiotensin III / Q000134:cerebrospinal fluid* / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004285:Dogs / Q000134:cerebrospinal fluid*; D004591:Electrophoresis, Polyacrylamide Gel; D051381:Rats; D012504:Saralasin / Q000494:pharmacology
D000804:Angiotensin II; D000805:Angiotensin III; D012504:Saralasin