Sensitive radioimmunological screening test for antithyroglobulin autoantibodies.
The test described here was designed as a screening test to be used in conjunction with the radioimmunoassay for human thyroglobulin. Anti-thyroglobulin autoantibodies, when present even in low concentrations, interfere with thyroglobulin determination. A 30-min incubation allows binding of 125I-thyroglobulin to endogenous anti-thyroglobulin autoantibodies. A subsequent 2-h incubation with goat anti-human gamma-globulin causes precipitation of such complexes. The immunoprecipitable radioactivity reflects the binding capacity of the sample for 125I-labeled thyroglobulin. Samples that are devoid of autoantibodies, and are therefore suitable for valid thyroglobulin determinations, bind less than 6% of the radiolabeled thyroglobulin. The sensitivity of this radioimmunoassay exceeds that of the tanned-erythrocyte hemagglutination test.
D001323:Autoantibodies / Q000032:analysis*; D001798:Blood Proteins / Q000378:metabolism; D006386:Hemagglutination Tests; D006801:Humans; D007457:Iodine Radioisotopes; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods; D013954:Thyroglobulin / Q000032:analysis / Q000097:blood / Q000276:immunology*; D013959:Thyroid Diseases / Q000097:blood / Q000276:immunology
D001323:Autoantibodies; D001798:Blood Proteins; D007457:Iodine Radioisotopes; D013954:Thyroglobulin