Isolation of a granulocyte colony inhibitory factor derived from human polymorphonuclear neutrophils.
An inhibitory factor obtained from mature human granulocytes which suppresses granulocyte and monocyte-macrophage colony formation by an action on the endogenous colony stimulating factor-producing cells has been partially purified and characterized. The methods for purification consisted of a combination of ultracentrifugation, DEAE-Sephadex chromatography, SDS polyacrylamide gel electrophoresis and isoelectric focusing. The material had a molecular weight range of 102--128 000 and an isoelectric point between pH 6.2 and 6.4. The inhibitory factor was found to be heat stable and glycoprotein in nature.
D002454:Cell Differentiation / Q000187:drug effects; D003864:Depression, Chemical; D006023:Glycoproteins / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D008264:Macrophages / Q000166:cytology; D009504:Neutrophils / Q000032:analysis*; D011506:Proteins
D006023:Glycoproteins; D008074:Lipoproteins; D011506:Proteins; C016313:colony inhibiting factor