Purification of the cytosol oestradiol-receptor complex from foetal guinea-pig uterus using electrofocusing on polyacrylamide plates.
The 3H-oestradiol receptor complex obtained from the cytosol fraction of foetal guinea-pig uterus was purified by the following steps: column chromatography in Sephadex G-15 and Ultrogel, and by electrofocusing on polyacrylamide plates. In the final step a concentration of 15-17% of the foetal uterine oestradiol receptor protein was obtained. The isoelectric point (pl) of this receptor was determined to be 6.1-6.2.
D000180:Acrylic Resins; D000818:Animals; D002850:Chromatography, Gel; D003600:Cytosol / Q000032:analysis*; D004958:Estradiol / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D005333:Fetus; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007525:Isoelectric Focusing / Q000379:methods; D011247:Pregnancy; D011960:Receptors, Estrogen / Q000302:isolation & purification* / Q000378:metabolism; D014599:Uterus / Q000032:analysis / Q000166:cytology*
D000180:Acrylic Resins; D011960:Receptors, Estrogen; D004958:Estradiol