The products of the "heat-shock" loci of Drosophila hydei. Correlation between locus 2-36A and the 70,000 MW "heat-shock" peptide.
Antibody was raised against total Drosophila hydei embryonic cellular protein with a molecular weight between 65,000 and 70,000 dalton. This antiserum reacted with the 70,000 MW "heat-shock" peptide found in "S labelled cell extracts of "heat-shocked" D. hydei tissue culture cells or salivary glands.--The antibody was coupled to Sepharose 4B and this material was used to absorb polysomes obtained from tissue culture cells incubated at 37 degrees C in the presence of tritiated RNA precursors. The relative concentrations of various RNA species complementary to the "heat-shock" loci 2-32A, 2-36A, and 2-48C in either bound, non-bound, or total polysomal material was then determined by in situ hybridization. The RNA species complementary to locus 2-36A was found to be enriched in the bound polysomal material.
D000818:Animals; D004330:Drosophila; D006358:Hot Temperature; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010455:Peptides; D011132:Polyribosomes; D012313:RNA / Q000096:biosynthesis*
D010455:Peptides; D012313:RNA