Alterations in number of protofilaments in microtubules assembled in vitro.
Tubulin from bovine brain was polymerized in vitro using a variety of assembly conditions. Many of the formed microtubules were shown to contain 14 wall protofilaments. The number of microtubules containing 14 protofilaments increased with consecutive repetitions of cold-dissociation followed by reassembly in vitro.
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D002417:Cattle; D006651:Histocytochemistry; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure*