Evidence of androgen and estrogen receptors in the preen gland of male ducks.
Androgen receptors have been found in duck preen glands by using dextran-coated charcoal adsorption. They bound DHT with high affinity (KD = 0.2 nM), limited capacity (45 fmoles/mgP) and good specificity. They sedimented at 8 S in a sucrose gradient and were destroyed by pronase digestion and heating. An estrogen receptor having different binding specificity was also demonstrated. On the basis of a marked annual cycle of gonadal activity in ducks, this system appears appropriate for studying the regulation of sex steroid hormone receptors.
D000327:Adsorption; D000818:Animals; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D002606:Charcoal; D003600:Cytosol / Q000032:analysis / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism; D004372:Ducks / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D011944:Receptors, Androgen / Q000302:isolation & purification* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000302:isolation & purification* / Q000378:metabolism; D011987:Receptors, Steroid / Q000302:isolation & purification*; D012627:Sebaceous Glands / Q000032:analysis* / Q000166:cytology / Q000378:metabolism
D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011987:Receptors, Steroid; D013196:Dihydrotestosterone; D002606:Charcoal; D004958:Estradiol