[Purification of the extracellular trypsin inhibitor from Actinomyces janthinus 118].
From the culture liquid filtrate of Actinomyces janthinus 118 a trypsin inhibitor (TI-AjI) was isolated by sulphate ammonium precipitation, DEAE-cellulose chromatography, QAE-Sephadex chromatography and two-fold crystallization. The resultant preparation was homogeneous during polyacrylamide gel electrophoresis and ultracentrifugation. As measured by gel filtration, the TI-AjI molecular weight was estimated to be 13,000+/-1,000. The culture liquid of Act. janthinus 118 was found to contain another substance with anti-trypsin activity that differed from TI-AjI in its molecular weight.
D003460:Crystallization; D008970:Molecular Weight; D013302:Streptomyces / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000032:analysis / Q000302:isolation & purification*
D014361:Trypsin Inhibitors
Ochistka vnekletochnogo ingibitora tripsina iz Actinomyces janthinus 118.