Platelet preservation. IV. Preparation and cryopreservation of platelet concentrates from fresh and overnight-stored human blood.
Two methods were used for the preparation of platelet concentrates from fresh and overnight-stored human ACD blood. In a two-step method prostoglandin E1 was added to platelet rich plasma and the mixture was centrifuged to obtain platelet concentrate. The IBM 2991 Blood Cell Processor was used in a one-step method. The concentrates were stored in the frozen state. Concentrates prepared with the two-step method had a higher platelet recovery and a lower leukocyte contamination than did concentrates prepared with the one-step method. Based on serotonin uptake volocity, response to hypotonic stress and available platelet factor 3, no essential differences were observed between platelet concentrates prepared from fresh and overnight-stored blood. Concentrates prepared with the one-step method had a higher serotonin uptake velocity than those prepared with the two-step method. This did not result in a better recovery after cryopreservation.
D001772:Blood Cell Count; D001792:Blood Platelets; D001793:Blood Preservation; D003080:Cold Temperature; D006801:Humans; D007038:Hypotonic Solutions; D007958:Leukocyte Count; D010977:Platelet Factor 3; D012701:Serotonin / Q000378:metabolism; D013997:Time Factors
D007038:Hypotonic Solutions; D012701:Serotonin; D010977:Platelet Factor 3