Enzymatic sulfation of steroids. II. The sulfation of corticosterone by the glucocorticoid sulfotransferases of rat liver cytosol.
A radioisotopic assay for the cytoplasmic corticosterone sulfotransferase activity of rat liver was developed. The steroid inhibits the enzyme reaction. For reliable results, a complex assay method, using three different corticosterone concentrations, each studied with several different amounts of enzyme, was necessary. This "mosaic" assay compensates for observed biological, gonadal and seasonal enzyme fluctuations. Cytosols from female rats contain 6--9-times the enzyme activity found in males. The sulfation product with both sexes is corticosterone-21-sulfate. The effects of castration and of androgen administration on hepatic cortisol and corticosterone sulfation were compared in female rats. Ovariectomy resulted in 20--32% and 25--35% decreases of hepatic corticosterone and cortisol sulfotransferase activity, respectively. Androgen administration caused 37--55% and 40--60% decreases of sulfation of the two steroids. The data suggest the equivalence of hepatic cortisol and corticosterone sulfotransferases. Fractionation of cytosols from female rats, on DEAE-Sephadex A-50 columns, resolved three peaks of corticosterone sulfotransferase activity which eluted concurrently with the hepatic cortisol sulfotransferases I, II and III. They appear to be the same enzymes. Cytosol from males contained cortisosterone sulfotransferase activity due mostly to sulfotransferase III. Sulfotransferases I and II appear to have higher turnover numbers for hepatic cortisol than for corticosterone. The reverse is true for sulfotransferase III.
D000818:Animals; D002369:Castration; D003348:Cortisone / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005260:Female; D006854:Hydrocortisone; D008099:Liver / Q000378:metabolism*; D008297:Male; D010053:Ovary / Q000502:physiology; D051381:Rats; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000032:analysis / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology
D013739:Testosterone; D013466:Sulfurtransferases; D015238:Sulfotransferases; C019937:corticosterone sulfotransferase; D003348:Cortisone; D006854:Hydrocortisone