Identification of topical photosensitizing agents in humans.
A method is described for the detection of topical photosensitizers in humans. Test agents were applied to the untanned midback under an occlusive dressing for 6 hr and then exposed to broad-spectrum radiation containing UV-A and visible wavelengths from a Xenon arc source. Well-known topical photosensitizers were readily identified. It was found that with certain poorly-penetrating substances, such as water-soluble dyes, applications to scarified skin were necessary to reveal phototoxic activity. In addition, these dyes were activated by wavelengths in the visible region of the spectrum.
D000287:Administration, Topical; D000328:Adult; D004396:Coloring Agents / Q000009:adverse effects; D003358:Cosmetics / Q000009:adverse effects; D006801:Humans; D008722:Methods; D010476:Perfume / Q000009:adverse effects; D010636:Phenols / Q000009:adverse effects; D010787:Photosensitivity Disorders / Q000139:chemically induced*; D012867:Skin / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
D004396:Coloring Agents; D003358:Cosmetics; D010476:Perfume; D010636:Phenols