Localization of inorganic ions by precipitative freeze dissolution.
A new method for localization of inorganic diffusible ions in tissue is introduced. It has been applied to localization of Tl+ and Rb+ in barley roots and is probably also suited for Cs+, Ca2+, Cl-, Br-, PO 3/4- and perhaps K+. Its principle consists of dissolution of the ice from frozen tissue in a concentrated aqueous solution of a precipitating agent that is kept at a temperature just above its melting point.
D000838:Anions; D002412:Cations; D005615:Freezing; D006651:Histocytochemistry; D001467:Hordeum / Q000378:metabolism; D008722:Methods; D010944:Plants / Q000378:metabolism* / Q000648:ultrastructure
D000838:Anions; D002412:Cations