Production of Methotrexate antiserum in rabbits: the significance of immunogen solubility, hapten content, and mode of administration of the antibody response.
A method for raising specific antibodies of high avidity against Methotrexate in rabbits is described. The antibody response was tested after intramuscular and after multi-site intradermal adminstration of three different immunogens. Antisera of high specificity and avidity were obtained by multi-site intradermal administration of a water-soluble immunogen with high hapten content (40 mol hapten/mol protein). A water-soluble immunogen with low hapten content (15-18 mol hapten/mol protein) resulted in antisera of high specificity but low avidity. No antibody response was obtained with a water-insoluble immunogen with high hapten content (40 mol hapten/mol protein).
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage; D006241:Haptens / Q000008:administration & dosage; D007271:Injections, Intradermal; D007273:Injections, Intramuscular; D008727:Methotrexate / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay; D012995:Solubility; D014867:Water
D000941:Antigens; D006241:Haptens; D014867:Water; D008727:Methotrexate