Co-replication of satellite DNA of Chironomus melanotus with mainband DNA during polytenization.
The DNAs of five Chironomus species, C. plumosus, C. nuditarsis, C. thummi thummi, C. melanotus, and Camptochironomus pallidivittatus, were investigated in analytical neutral isopycnic CsCl density gradients. DNA was isolated both from larval brains ("diploid-DNA") and salivary glands ("polytene-DNA"). The buoyant densities of mainband DNAs were 1.692 g/cm3, with the exception of C. melanotus whose mainband had a density of 1.693 g/cm3. The densities correspond to a calculated GC content of 33% and 34% respectively. Only in C. melanotus was the DNA clearly resolved into mainband DNA and two distinct satellite shoulders: Satellite I, with a buoyant density of 1.684 g/cm3 (25% GC, calculated) and satellite II of 1.679 g/cm3 (19% GC, calculated). The two satellites comprise 15% of the total DNA in the "diploid-DNA"' and they also occur in the "polytene-DNA", where they make up 11%. The results are discussed in the general context of under- and overreplication in polyploid and polytene cells.
D000818:Animals; D001923:Brain Chemistry; D002500:Centrifugation, Isopycnic; D002875:Chromosomes / Q000032:analysis; D004247:DNA / Q000096:biosynthesis* / Q000302:isolation & purification; D004261:DNA Replication; D004276:DNA, Satellite / Q000096:biosynthesis* / Q000302:isolation & purification; D004175:Diptera / Q000235:genetics*; D008970:Molecular Weight; D012469:Salivary Glands / Q000032:analysis
D004276:DNA, Satellite; D004247:DNA