[Comparison of free ribonucleotide pool in the spleens of C57BL and DBA/2 mice and in leukemia cells sensitive and resistant to 5-fluorouracil].
The amount of free purine and pyrimidine ribonucleotides in the spleens of mice (C57Bl and DBA/2) and in lympholeukemia cells (La and L1210), sensitive and with induced resistance to 5-fluorouracil, was determined by chromatography on a column with DEAE-cellulose. It was found that the cytidine ribonucleotide pool in the spleens of DBA/2 mice is 2 times lower as compared to C57Bl mice. The lympholeukemia cells (La and L1210) isolated from the animals also differed in their uridine nucleotide pools. The development of leukemia was accompanied by a decrease in ATP and GTP. No significant changes in the total amount of pyrimidine nucleotides under developing resistance to 5-fluororuacil were observed.
D000818:Animals; D005472:Fluorouracil / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011685:Purine Nucleotides / Q000378:metabolism; D011742:Pyrimidine Nucleotides / Q000378:metabolism; D012265:Ribonucleotides / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism*
D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D012265:Ribonucleotides; D005472:Fluorouracil
Sravnenie fonda svobodnykh ribonukleotidov v selezenke mysheĭ C57BL i DBA/2 i v kletkakh leĭkozov, chuvstvitel'nykh i ustoĭchivykh k 5-ftoruratsilu.