[Content of SH-groups in some fractions of rat hemoglobin].
Eight fractions of rat hemoglobin obtained by electrophoresis in polyacrylamide gel were studied. Having used parachloromercuric benzoate (PChMB) and 5,5-dithio-bis (2-nitrobenzoic acid) it was established that fractions of 3 and 3a carboxyhemoglobin contain 6 available SH-groups and the rest one (1, 2, 2a, 4, 5, 6) about 4 SH-groups. In all fractions only 2 masked SH-groups are found. A problem is discussed on SH-groups localization in the polypeptide chains of certain hemoglobin fractions in rats.
D000818:Animals; D001797:Blood Protein Electrophoresis; D002729:Chloromercuribenzoates; D003545:Cysteine / Q000032:analysis*; D004228:Dithionitrobenzoic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins; D051381:Rats
D002729:Chloromercuribenzoates; D006454:Hemoglobins; D004228:Dithionitrobenzoic Acid; D003545:Cysteine
Soderzhanie SH-grupp v otdel'nykh fraktsiiakh gemoglobina krys.