The "Southmead System," a simple, fully-automated, continuous-flow system for immunoassays [Appendix: application to serum thyroxine radioimmunoassay].
A simple, fully-automated, continuous-flow system suitable for radioimmunoassay, enzyme immunoassay, and fluoro-immunoassay is described. The system is versatile, inexpensive, and requires only equipment and skills that already are available in most clinical chemistry laboratories. In this system, the antibody is covalently linked to solid-phase, porous particles of agarose (Sepharose, size 40-70 micrometer in the dry form). After sampling and mixing the assay reactants, the assay "cocktail" containing the solid-phase-bound antigen and the unbound fraction is directed to the separation block. Separation is achieved by use of a highly porous membrane (pore size, about 10 micrometer) and by controlling the volume of inflow and outflow a fixed and precise amount of fluid is filtered through the membrane containing only the free (unbound fraction), which can then be quantitated. The percentage of unbound fraction misclassified is negligible (less than 1.0%). Application of these principles to the assay of serum thyroxine is described in an appendix.
D001322:Autoanalysis / Q000295:instrumentation; D005069:Evaluation Studies as Topic; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D011863:Radioimmunoassay / Q000295:instrumentation*; D013974:Thyroxine / Q000097:blood*; D013997:Time Factors
D013974:Thyroxine