Immunoradiometric assay of salivary anti-c3 antibodies.
By an immunoradiometric assay IgA and IgG anti-C3 antibodies were found in adult parotid salivas. The binding of the IgA antibodies to complement coated red cells could be reversed by washing in buffer containing 0.01 M EDTA but IgG antibodies were unaffected. The binding of IgA antibodies was inhibited by small quantities of purified C3. This assay may prove useful in the detection of anti-C3 antibodies in other body fluids.
D000328:Adult; D000906:Antibodies / Q000032:analysis*; D003176:Complement C3; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007231:Infant, Newborn; D010306:Parotid Gland; D012463:Saliva / Q000276:immunology*
D000906:Antibodies; D003176:Complement C3; D007070:Immunoglobulin A; D007074:Immunoglobulin G