Isolation of messenger RNA coding for eggshell protein of the DNA-eliminating nematode Ascaris lumbricoides.
Poly(A)-containing RNA from polyploid uterine epithelial cells of Ascaris lumbricoides has been isolated by poly(U)-Sepharose chromatography. The bulk of poly(A)-containing RNA migrates as 18-S RNA in formamide/polyacrylamide gels. In a cell-free wheat germ system, this RNA directs the synthesis of a polypeptide with identical migration behavior in dodecylsulphate/urea/polyacrylamide gels as the polypeptide isolated from the proteinaceous eggshell. The two proteins reveal almost identical peptide patterns in fingerprint analysis. The authentic eggshell protein has been identified as a glycoprotein with a molecular weight of about 10000, as determined by dodecylsulphate/polyacrylamide gel electrophoresis. The apparent discrepancy between mRNA length and the required coding length for the protein is discussed.
D000818:Animals; D001200:Ascaris / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004527:Egg Proteins / Q000096:biosynthesis*; D004528:Egg Shell; D004848:Epithelium / Q000378:metabolism; D005260:Female; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D011061:Poly A; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism; D014599:Uterus / Q000378:metabolism
D004527:Egg Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA