Automated measurement of serum thyroxine with the "ARIA II," as compared with competitive protein binding and radioimmunoassay.
Two conventional serum thyroxine assays, run in separate laboratories, one by competitive protein binding and one by radioimmunoassay, were used to evaluate the automated ARIA II (Becton Dickinson Immunodiagnostics) serum thyroxine assay. Competitive protein binding as compared to ARIA II with 111 clinical serum samples gave a slope of 1.04 and a correlation coefficient of 0.94. The radioimmunoassay comparison to ARIA II with 53 clinical serum samples gave a slope of 1.05 and a correlation coefficient of 0.92. The ARIA II inter-assay coefficient of variation for 10 replicates of low, medium, and high thyroxine serum samples was 6.2, 6.0, and 2.9%, respectively, with an inter-assay coefficient of variation among 15 different assays of 15.5, 10.1, and 7.9%. The automated ARIA II, with a 2.2-min cycle per sample, gives results that compare well with those by manual methodology.
D000918:Antibody Specificity; D001667:Binding, Competitive; D003429:Cross Reactions; D006801:Humans; D008722:Methods; D011485:Protein Binding; D011863:Radioimmunoassay; D011864:Radioimmunosorbent Test; D013974:Thyroxine / Q000097:blood*
D013974:Thyroxine