[Improvement of the method of collection of blood flowing from the adrenal glands in the experiment on dogs].
Blood from the adrenal gland can flow in two directions--into the caudal vena cava and by anastomoses into the portal vein. A method of multiple blood procuring from these vessels in dogs for the purpose of studying the adrenal gland function is suggested. Polyethylene catheters, 0.9 mm in diameter and 35--40 mm in length glued to capron plates, mandrins, Gordeev's needles and blood-drawing needles are necessary. Transperitoneal surgical approach to the caudal and mesenteric veins is to be provided. Purse-string suture is applied on the wall of the mesenteric vein. The catheter is washed with heparin, the mandrin is introduced into it and it is inserted into the Gordeev's needle. A puncture is made in the centre of the purse-string suture with this needle, which is introduced into the vein together with the catheter. Gordeev's needle is withdrawn and the catheter is pushed through to the portal vein. The purse-string suture is tightened, and the capron plate is fixed in the wall of the vein. The mandrin is recovered from the catheter, heparin is administered into its canal, polyethylene plug is inserted and withdrawn through the abdominal wall outside with the aid of the blood-drawing needle. The catheter is introduced into the caudal vena cava in the same way. Blood samples can be obtained on the 14th postoperative day.
D000311:Adrenal Glands / Q000098:blood supply*; D000818:Animals; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods*; D002404:Catheterization / Q000295:instrumentation; D004285:Dogs; D008642:Mesenteric Veins; D011169:Portal Vein; D014682:Vena Cava, Inferior
O sovershenstvovanii metoda vziatiia krovi, ottekaiushcheĭ ot nadpochechnikov, v eksperimente na sobakakh.