Preparation of antisera to three individual rat neurophysins and their use for radioimmunoassays.
A number of antisera have been raised against individual rat neurophysins. One of these proved suitable for the specific radioimmunoassay of rat vasopressin-neurophysin and data validating such a use are presented. Use of this antiserum showed that vasopressin-neurophysin is present in rat neurohypophyses in equimolar amounts to vasopressin, while in the hypothalamus the assay detected twice as much neurophysin-like material as hormone. A number of partially specific antisera for rat oxytocin-neurophysin have been obtained and the best of these was raised against the minor neurophysin.
D000818:Animals; D000917:Antibody Formation; D007031:Hypothalamus / Q000032:analysis; D007106:Immune Sera; D009481:Neurophysins / Q000032:analysis / Q000276:immunology*; D010904:Pituitary Gland, Posterior / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D014667:Vasopressins / Q000032:analysis
D007106:Immune Sera; D009481:Neurophysins; D014667:Vasopressins