Plasma glucose measurement with the Yellow Springs Glucose Analyzer.
The Yellow Springs Glucose Analyzer, a device for the quantitative measurement of glucose concentrations, involves the use of immobilized glucose oxidase, incorporated on a membrane covering a hydrogen peroxide sensor. Operation of the instrument is simple. After an initial calibration, 25 microliter of plasma is injected into a reaction chamber. At 45 s a digital result for glucose is displayed. The within-batch coefficient of variation for the method is 1.2% or less for glucose concentrations of 0.94 to 3.98 g/liter. The between-batch coefficient of variation is 5.8% or less for glucose concentrations of 0.29 to 2.91 g/liter. Concentration and readout are linearly related to at least 4.6 g/liter. Analytical recoveries ranged from 100 to 102%. Carry-over was negligible. Values for glucose concentration obtained with the instrument compared well (r = 0.997) with those obtained with the Beckman Glucose Analyzer.
D001322:Autoanalysis; D001786:Blood Glucose / Q000032:analysis*; D004566:Electrodes; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D006801:Humans; D008722:Methods
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase