Steroid-protein interactions. 40. The effect of fatty acids on progesterone binding to human serum albumin.
Human serum albumin was delipidated by solvent extraction or by treatment with charcoal. Progesterone complexes formed with these albumin preparations had higher association constants than those formed with the untreated samples. The charcoal method of delipidation resulted in somewhat higher affinity constants than extraction with chloroform/methanol. Addition of 5 mol lauric acid per mol albumin reduced the association constant of the progesterone complex by approx. 50%. Studies with lauric, myristic, and palmitic acid showed that the decrease of binding affinity for progesterone was proportional to the amount of fatty acid added to albumin, and to its chain length. These results confirm and extend our previous findings of inhibition of progesterone binding to human albumin by long-chain fatty acids.
D001665:Binding Sites; D003864:Depression, Chemical; D005227:Fatty Acids / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D011374:Progesterone / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D012709:Serum Albumin / Q000378:metabolism*; D013329:Structure-Activity Relationship
D005227:Fatty Acids; D012709:Serum Albumin; D011374:Progesterone