Preparation of radioactive iodinated cholylhistamine for use in the radioimmunoassay of cholic acid.
A major handicap in the development of simple and accurate radioimmunoassay procedures for bile acids has been the lack of a radioactive standard of high specific activity. To provide such a compound, we first synthesized cholylhistamine using the carbodiimide reaction. The hypothesized structure was confirmed by elemental analysis, thin-layer chromatography, infrared and mass spectral analysis. The cholylhistamine was then iodinated with 125I, using the choloramine-T method. The 125I-cholylhistamine was bound by antisera raised against a cholic acid-bovine serum albumin conjugate. This procedure should prove useful in preparing radioactive conjugates for all of the bile acids.
D002793:Cholic Acids / Q000032:analysis / Q000138:chemical synthesis*; D002855:Chromatography, Thin Layer; D006632:Histamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D007457:Iodine Radioisotopes; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods
D002793:Cholic Acids; D007457:Iodine Radioisotopes; C016306:cholylhistamine; D006632:Histamine