I. Increase in bone marrow CFU-S and CFU-C after in vivo phytohaemagglutinin (PHA) administration in mice.
An absolute increase in bone marrow CFU-S was observed within 4 hours after intraperitoneal administration of 3 mg PHA-M or 250 microgram PHA-P. At that time, there was no increase in spleen CFU-S, while a pronounced decrease in the number of circulating CFU-S was noted. A two to fourfold increase in bone marrow CFU-C was found within 12 hours after intraperitoneal administration of a similar dose of PHA-M or PHA-P. Addition of PHA-M or PHA-P at small concentrations resulted in a marked inhibition of granulocytic colony formation whereas macrophage colony growth became depressed only at very high concentrations.
D000818:Animals; D001772:Blood Cell Count; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006400:Hematocrit; D007958:Leukocyte Count; D051379:Mice; D008809:Mice, Inbred C3H; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012156:Reticulocytes; D013154:Spleen / Q000166:cytology
D010835:Phytohemagglutinins