Factors affecting glucosyl and mannosyl transfer to dolichyl monophosphate by liver cell-free preparations.
GDP-mannose and UDP-mannose (each at less than 1 micrometer) markedly inhibit glucosyl transfer from UDP-glucose (1.6 micrometer( to dolichyl phosphate in liver microsomal preparations. The biphasic response suggests the presence of two glucosyl transferases only one of which is inhibited. The inhibition appears to be a property of the intact nucleotide phosphate sugars and not due to competition for a limited pool of dolichyl phosphate. UDP-galactose and UDP-xylose cause a less marked inhibition of the same enzyme. The failure of UDP-glucose to inhibit mannosyl transfer suggests that the pool of dolichol monophosphate used by mannosyl transferase is not available to the glucosyl transferase. The relationship between the degree to which an exogenous prenol phosphate acts as an acceptor of mannose and the degree to which it inhibits mannosylation of endogenous dolichyl monophosphate varies among different prenyl phosphates. Mannosyl transferase exhibits two pH optima.
D000818:Animals; D002413:Cations, Divalent; D004287:Dolichol Monophosphate Mannose / Q000096:biosynthesis*; D004288:Dolichol Phosphates / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D007700:Kinetics; D008364:Mannosyltransferases / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology*; D009702:Nucleoside Diphosphate Sugars / Q000494:pharmacology; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D051381:Rats; D012265:Ribonucleotides / Q000494:pharmacology; D013552:Swine
D002413:Cations, Divalent; D004288:Dolichol Phosphates; D009702:Nucleoside Diphosphate Sugars; D011105:Polyisoprenyl Phosphate Sugars; D011106:Polyisoprenyl Phosphates; D012265:Ribonucleotides; D004287:Dolichol Monophosphate Mannose; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D008364:Mannosyltransferases