Heterogeneity of rat rib chondroitin sulfate and susceptibility to rat gastric chondrosulfatase.
Cartilage chondroitin sulfate isolated directly from rat rib or from in vitro culture of rat rib constitutes a population of glycosaminoglycans which is heterogeneous with respect to size, degree of sulfation and content of N-acetyl-galactosamine 4-sulfate. Fractions, elute from Dowex-1 in order of increasing molecular size and degree of sulfation up to a certain limit. Unsulfated disaccharides and disulfated disaccharides are present in both the undersulfated chondroitin sulfate fractions and in the average or more representative chondroitin sulfate. A small content of disccharide 6-sulfate is present in all fractions and appears to be an integral part of the chondroitin 4-sulfate molecules. Rat gastric chondrosulfatase hydrolyzes sulfate preferentially from the larger chondroitin 4-sulfate molecules, and the sulfate is removed primarily from the disaccharide 4-sulfate units.
D000818:Animals; D002356:Cartilage / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002807:Chondroitin / Q000031:analogs & derivatives; D002808:Chondroitin Lyases / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000302:isolation & purification / Q000378:metabolism; D002810:Chondroitinases and Chondroitin Lyases / Q000378:metabolism*; D002811:Chondroitinsulfatases / Q000378:metabolism*; D046508:Culture Techniques; D008297:Male; D051381:Rats; D012272:Ribs; D013270:Stomach / Q000201:enzymology; D013379:Substrate Specificity
D002807:Chondroitin; D002809:Chondroitin Sulfates; D002811:Chondroitinsulfatases; D002808:Chondroitin Lyases; D002810:Chondroitinases and Chondroitin Lyases