Electron microscopic localization of DNA-dependent RNA polymerase binding sites on DNA using enzyme immobilized on colloidal gold.
DNA dependent RNA polymerase has been coupled to gold granules of 60 A. The gold-polymerase complex (Au-P) has been demonstrated to bind to chick oviduct DNA and be capable of transcription as monitored by both biochemical activity and electron microscopy. Binding of Au-P to DNA is competable with free polymerase.
D001665:Binding Sites; D003102:Colloids; D004247:DNA / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004800:Enzymes, Immobilized; D006046:Gold; D008854:Microscopy, Electron; D014158:Transcription, Genetic
D003102:Colloids; D004800:Enzymes, Immobilized; D006046:Gold; D004247:DNA; D012321:DNA-Directed RNA Polymerases