A simplified method for rapid determination of porphyrins by thin-layer chromatography.
Free porphyrins, especially copro-, uro-, and protoporphyrin, were successfully separated by talc thin-layer chromatography (TLC), and were determined from the relationship of the weight and the area of porphyrins in the spot on the thin-layer plate. The results obtained from both the solvent extraction and the simple, rapid TLC method reported in this paper showed good correlation for coproporphyrin levels up to 400 microgram/1. The TLC method was applied to the determination of urinary coproporphyrin in lead workers and in rats.
D000328:Adult; D000818:Animals; D002617:Chemical Industry; D002855:Chromatography, Thin Layer / Q000379:methods; D003306:Coproporphyrins / Q000652:urine; D004781:Environmental Exposure; D006801:Humans; D007564:Japan; D007854:Lead; D009784:Occupational Diseases / Q000652:urine; D011166:Porphyrins / Q000652:urine*; D051381:Rats
D003306:Coproporphyrins; D011166:Porphyrins; D007854:Lead