Composition and synthesis of three higher ganglioside homologs in bovine mammary tissue.
Three higher gangliosides were identified as constituents of bovine mammary gland. The structures of these three gangliosides were shown to be ceramide-glucose-galactose-(sialic acid)-N-acetylgalactosamine-galactose, ceramide-glucose-galactose-(sialic acid)2-N-acetylgalactosamine, and ceramide-glucose-galactose-(sialic acid)2-N-acetylgalactosamine-galactose. These gangliosides accounted for only a small fraction (less than 20%) of the lipid-bound sialic in mammary gland. White fatty acids with even carbon numbers from C14 to C26 were predominant in these gangliosides, they also contained C23 and C25 fatty acids. Mammary gland Golgi apparatus-rich fractions had all glycosyltransferases required for synthesis of these gangliosides starting with ceramide.
D000818:Animals; D002417:Cattle; D005260:Female; D005732:Gangliosides / Q000032:analysis* / Q000096:biosynthesis; D008321:Mammary Glands, Animal / Q000032:analysis* / Q000378:metabolism; D008892:Milk / Q000032:analysis
D005732:Gangliosides