Tartrate-resistant (band 5) acid phosphatase activity measured by electrophoresis on acrylamide gel.
A tartrate-resistant acid phosphatase was isolated from a huma spleen infiltrated with reticulum cells of leukemic reticuloendotheliosis. The purified enzyme was used to establish the optimal conditions for quantitative analysis of this enzyme by electrophoresis on acrylamide gel. As little as 0.1 unit of enzyme activity (or 1 ng of the purified enzyme protein) could be quantitatively detected when it was mixed with 4 mg of albumin before electrophoresis.
D000135:Acid Phosphatase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000201:enzymology; D009994:Osmolar Concentration; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013154:Spleen / Q000201:enzymology; D013644:Tartrates; D013997:Time Factors
D013644:Tartrates; D012710:Serum Albumin, Bovine; D000135:Acid Phosphatase