The synthesis of presumptive procollagen messenger ribonucleic acid in the calvaria of the developing chick embryo.
The presumptive messenger RNAs for type I procollagen were isolated from chick embryo calvaria at various stages of development. Poly(A)-containing RNA fractions from denaturing sucrose gradients directed protein synthesis in a cell-free system derived from wheat germ. Procollagen mRNA activity was detected in a region of about 26 S. Approx. 80% of the labeled proline incorporated into cell-free product was susceptible to digestion by purified bacterial collagenase. The synthesis of procollagen mRNAs was followed during development. Comparison of the in vitro labeled mRNAs from calvaria of day 12--16 embryos indicated that the 26 S component was most pronounced at day 13 and decreased progressively towards day 16. In addition, incubation of calvaria with tritiated nucleosides for 1.5--25 h revealed that 26 S mRNA was significantly labeled only after prolonged periods. The results suggest that procollagen mRNA is a relatively stable species with a prolonged half-life compared to the majority of mRNAs in this tissue.
D000818:Animals; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism*; D002474:Cell-Free System; D002642:Chick Embryo; D046508:Culture Techniques; D011061:Poly A / Q000378:metabolism; D011347:Procollagen / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification
D011347:Procollagen; D012333:RNA, Messenger; D011061:Poly A