Separation of the natural retinoids by high-pressure liquid chromatography.
A reverse phase high-pressure liquid chromatography system for rapid separation of various retinoids (vitamin A and its analogs) with little or no degradation is described. This method permits detection of as little as 22 pmol of retinoic acid. The procedure has been applied to the study of retinoic acid metabolism in vitamin A-deficient hamsters.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006224:Cricetinae; D008647:Mesocricetus; D008968:Molecular Conformation; D012172:Retinaldehyde / Q000302:isolation & purification; D014212:Tretinoin / Q000302:isolation & purification; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D014802:Vitamin A Deficiency / Q000378:metabolism
D014801:Vitamin A; D014212:Tretinoin; D012172:Retinaldehyde