[Translocation mechanism of ribosomes].
The paper summarizes studies of the molecular mechanism of the dynamic function of the ribosome, i. e. translocation, performed in the author's laboratory during the past decade. The hypothesis of the locking-unlocking of the ribosomal subparticles and the kinematical model of the working ribosome, the processes of spontaneous (factor-free) and factor-dependent translocation, the sequence of events in the factor-dependent translocation, the energetics of translocation and the contribution of the elongation factors with GTP are considered. The following conclusions are made: (1) the translocation mechanism is intrinsic to the structural organization of the ribosome itself but not introduced by the protein elongation factors; (2) the transpeptidation reaction is one of the sources of energy for the work of the translocation mechanism; (3) the protein elongation factors with GTP impart additional energy to the ribosome, including that for translocation, and thus ensure excess power which is realized, in particular, in the increase of the translocation rate and its resistance against inhibitors and hindrances; (4) the promoting role of the elongation factors with GTP does not proceed by a direct conjugation of GTP hydrolysis with translocation, but through the affinity of the elongation factors to the ribosome, with a subsequent compensation of the affinity at the expense of GTP cleavage.
D006160:Guanosine Triphosphate / Q000502:physiology; D007700:Kinetics; D010441:Peptide Chain Elongation, Translational; D010445:Peptide Elongation Factors / Q000502:physiology; D012270:Ribosomes / Q000378:metabolism* / Q000502:physiology; D013816:Thermodynamics; D014178:Translocation, Genetic
D010445:Peptide Elongation Factors; D006160:Guanosine Triphosphate
Translokatsionnyĭ mekhanizm ribosomy.