Identification of rosette-forming cells in permanent preparations.
A method is described for the identification of leucocytes in permanent cytocentrifuge rosette preparations made with the fluorescent methyl green pyronin-SITS (MPS) staining technique. The percentages of rosettes in stained slides agree well with the results obtained with the conventional haemocytometer method. Application of the MPS staining to cytocentrifuge preparations permits reliable morphological differentiation between free and rosetting leucocytes, and should be particularly valuable in studies on lymphocyte subpopulations in patients.
D002498:Centrifugation; D003165:Complement System Proteins; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D008739:Methyl Green; D011754:Pyronine; D012397:Rosette Formation; D013451:Sulfonic Acids
D013451:Sulfonic Acids; D008739:Methyl Green; D003165:Complement System Proteins; D011754:Pyronine