DNA damage during the peroxidase-catalyzed aerobic oxidation of isobutanal.
The aerobic oxidation of isobutanal catalyzed by peroxidase, when carried out in the presence of DNA, produces alkali-sensitive bonds in this macromolecule. Neither the initial components of this reaction nor the final stable products are responsible for this effect. Since triplet acetone has been recently identified as an intermediate in this oxidation (Durán, N., Faria Oliviera, O.M.M., Haun, M. and Cilento, G. (1977) J. Chem. Soc. Chem. Commun., 442--443), this species is a likely candidate for the entity which brings about the lesions, via transfer of its electronic energy to DNA.
D000332:Aerobiosis; D000447:Aldehydes; D002460:Cell Line; D004247:DNA; D006735:Horseradish Peroxidase; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010544:Peroxidases
D000447:Aldehydes; D004247:DNA; C017439:isobutyraldehyde; D006735:Horseradish Peroxidase; D010544:Peroxidases