Initial investigations into adrenaline accumulation and adrenergic responsiveness in cultured neonatal rat heart cells.
1 Accumulation of tritiated adrenaline (and/or its products) has been studied by a variety of techniques in viable, attached and free-floating myoblasts cultured from neonatal rat heart cells. 2 Accumulation increased with the number of cells used, the culture age of cells, incubation time and the concentration of adrenaline in the incubation mixture. 3 Accumulation-concentration data were not linearly correlated with dose-response data. 4 Accumulation did not correlate well with beta-adrenoceptor responsiveness in a number of situations of varying responsiveness. 5 Accumulation was not blocked by a variety of uptake blocking agents or alpha- and beta-adrenoceptor antagonists. 6 A much smaller accumulation process was observed in fibroblasts which respond to adrenaline with reversible morphological changes.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004837:Epinephrine / Q000378:metabolism* / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
D004837:Epinephrine