Gas chromatography of 5-hydroxy-3-methylindole in human urine.
Electron capture gas chromatography was used to estimate the urinary 5-hydroxy-3-methylindole in epileptic patients and normal controls. The urine was filtered, concentrated in vacuo, extracted into benzene-ethanol and passed through a silica gel column. The dried eluate was dissolved in acetonitrile, tri-fluoroacetylated and estimated by gas chromatography as di-trifluoroacetylated 5-hydroxy-3-methylindole. This structure was confirmed by gas chromatography-mass spectrometry. 5-Hydroxy-3-methylindole was excreted at high concentrations in some epileptics and at low concentrations in normal control subjects.
D000293:Adolescent; D000328:Adult; D002849:Chromatography, Gas; D004827:Epilepsy / Q000652:urine; D005260:Female; D006801:Humans; D007211:Indoles / Q000652:urine*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D012862:Skatole / Q000031:analogs & derivatives
D007211:Indoles; C016504:5-hydroxy-3-methylindole; D012862:Skatole