Immunosuppression in new-born lambs.
A novel technique, based on cytotoxicity-neutralization, was developed for the in vitro titration of anti-sheep lymphocyte and anti-sheep macrophage sera. The titres obtained for a number of antisera were compared with those found in an agglutination assay. Anti-lymphocyte sera with a high cytotoxicity-neutralization titre very effectively suppressed the number of circulating lymphocytes in the peripheral blood of treated new-born lambs, thus indicating in vivo immunosuppressive activity.
D000372:Agglutination Tests; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000961:Antilymphocyte Serum / Q000494:pharmacology; D003520:Cyclophosphamide / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D007106:Immune Sera / Q000494:pharmacology; D007165:Immunosuppression Therapy; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D009500:Neutralization Tests; D012756:Sheep / Q000276:immunology*
D000961:Antilymphocyte Serum; D007106:Immune Sera; D003520:Cyclophosphamide