Immunoadjuvant activities of synthetic 6-O-acyl-N-acetylmuramyl-L-alanyl-D-isoglutamine with special reference to the effect of its administration with liposomes.
Addition of a lauroyl, stearoyl or docosanoyl group to the primary hydroxy group at the C-6 position of N-acetylmuramyl-L-alanyl-D-isoglutamine gave lipophilic derivatives that had definite adjuvancies in induction of delayed-type hypersensitivity and enhancement of antibody production against a test protein antigen, ovalbumin, when administered to guinea pigs as liposomes, that is without mineral oil. When administered as mineral oil-in-water emulsion, including Ribitype emulsions, rather than as water-in-mineral oil emulsions, N-acetylmuramyl-L-alanyl-D-isoglutamine and its 6-O-acyl derivatives showed only weak immunoadjuvancies.
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000917:Antibody Formation; D004655:Emulsions; D006020:Glycopeptides / Q000276:immunology*; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular; D008081:Liposomes; D009821:Oils; D013329:Structure-Activity Relationship
D000276:Adjuvants, Immunologic; D004655:Emulsions; D006020:Glycopeptides; D008081:Liposomes; D009821:Oils