Differential borohydride assay for conjugated ketosteroids: improvement with propylene glycol.
A previously developed differential UV assay for 3-one-4-ene steroids involving reduction with sodium borohydride was frequently found to be less than 100% complete. Such incomplete reduction can be mimicked by additions of sodium metaborate, a product of the hydrolysis of sodium borohydride, to the reaction system. Complete reduction can be achieved by adding propylene glycol to the reduction system. The phenomenon was studied using halcinonide as a model.
D000287:Administration, Topical; D000893:Anti-Inflammatory Agents / Q000032:analysis; D001894:Borohydrides; D002855:Chromatography, Thin Layer; D005938:Glucocorticoids; D010084:Oxidation-Reduction; D011282:Pregnenediones / Q000032:analysis; D011409:Propylene Glycols
D000893:Anti-Inflammatory Agents; D001894:Borohydrides; D005938:Glucocorticoids; D011282:Pregnenediones; D011409:Propylene Glycols