The adenylate cyclase system of planaria Polycelis tenuis: activation by serotonin and guanine nucleotides.
The particulate fraction prepared after homogenization of planaria Polycelis tenuis in a buffer containing 3 mM EDTA and 15 mM 2-mercaptoethanol possesses an adenylate cyclase activity which was enhanced two-fold by serotonin and 20-fold by the nucleotide analog guanosine 5'-(beta-gamma-imino)triphosphate, Gpp(NH)p; when present together, the two activators exhibited a marked synergistic effect. The effect of serotonin was dose dependent, with a KA of 2 micrometer and a Hill coefficient of 0.4. In the presence of 10 micrometer Gpp(NH)p, these values became 45 nM and 1.5, respectively. The effect of serotonin was due to an increase in the maximal velocity of the enzyme and was specifically inhibited by methiotepin. The effect of methiotepin was half-maximal at 0.2 micrometer in the absence of Gpp(NH)p and at 5.0 micrometer in its presence. Planaria thus appear to be the lowest organisms in which guanine nucleotides are active upon adenylate cyclase. As serotonin is normally present in planaria, it is postulated that a serotonin-dependent regulation of adenylate cyclase activity plays a physiological role in this species.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D004357:Drug Synergism; D004789:Enzyme Activation / Q000187:drug effects; D006150:Guanine Nucleotides / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008719:Methiothepin / Q000494:pharmacology; D010932:Planarians / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology*; D013045:Species Specificity; D014419:Turbellaria / Q000378:metabolism*
D006150:Guanine Nucleotides; D012701:Serotonin; D006165:Guanylyl Imidodiphosphate; D008719:Methiothepin; D006160:Guanosine Triphosphate; D000262:Adenylyl Cyclases