A simple method for the isolation of murine peripheral blood lymphocytes.
A simple, rapid and reproducible semi-microtechnique was developed for the isolation of murine peripheral blood lymphocytes. Murine peripheral blood was collected from brachial vessels and centrifuged through a Ficoll--Hypaque gradient in a microfuge tube yielding 1--1.5 X 10(6) lymphocytes per mouse.
D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005260:Female; D008214:Lymphocytes; D051379:Mice; D008805:Mice, Inbred A