Epinephrine-sensitive adenylate cyclase of human fat cell ghosts. Modulation of enzyme activity by GTP.
Some of the effects of GTP on human fat cell adenylate cyclase activity were studied. This nucleotide caused a dose-dependent inhibition of basal activity without affecting the hormone-activated rate of cAMP formation. Maximal effects were observed at GTP-concentrations of about 1 x 10(-4) M. The relative extent of hormonal stimulation was about 1.5-fold increased in the presence of 0.1 mM GTP.
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism; D000273:Adipose Tissue / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D006801:Humans; D008274:Magnesium / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D006160:Guanosine Triphosphate; D000262:Adenylyl Cyclases; D008274:Magnesium; D004837:Epinephrine