[Synthesis and properties of carrier-fixed enzymes. VIII. Kinetic studies of the binding velocity of enzymes to macroporous carriers].
The kinetics of binding the enzymes glucose oxidase, trypsin, and peroxidase and also bovine gamma-globulin to the macroporous carriers dialdehyde cellulose, diazotized amino polystyrene, acrolein-acrylamide copolymer, and isothiocyanate groups carrying CPG-glass was studied. It was found empirically that the increase in protein content and--if there occurred no inactivation of enzyme during binding reaction--of enzymatic activity of the enzyme-carrier complex with the reaction time can be described by saturation curves (first order hyperbolas), which represent straight lines after suitable transformation. Thereby it is possible to calculate protein content or specific activity of the enzyme-carrier complex for any moment during binding reaction. From intercepts of the obtained straight lines with the ordinate and with the abscissa, respectively, one can determine the maximum binding capacity and the maximum specific activity and from their slope the reaction rate can be obtained. If there result no straight lines for plotting enzymatic activity of the enzyme-carrier complex against binding time after transformation, then this is an indication for partial inactivation of the free or already insolubilized enzyme. From kinetic measurements it is concluded that in all cases studied by use the preceding stage in chemical fixation of the enzyme to carrier is an adsorption equilibrium. Limitation of reaction rate by diffusion as in loading an ion exchanger could not be observed.
D005898:Glass; D005949:Glucose Oxidase; D010084:Oxidation-Reduction; D010544:Peroxidases; D011485:Protein Binding; D013997:Time Factors; D014357:Trypsin
D005949:Glucose Oxidase; D010544:Peroxidases; D014357:Trypsin
Synthese und Eigenschaften trägerfixieter Enzyme. VIII. Kinetische Untersuchunge über die Geschwindigkeit der Bindung von Enzymen an makroporöse Träger.