Isolation and characterization of the predominant protein in nuclear ribonucleoprotein particles from rat liver.
The predominant protein of the nuclear ribonucleoprotein particles of rat liver was isolated by polyacrylamide gel electrophoresis. The polypeptide represented 35% to 40% of the total mass of the protein moiety. Its molecular weight was estimated to be 38 000 and its NH2-terminal residue was found to be threonine. The amino acid composition is unique in having a high content of glycyl residues (20%) and NG-dimethylarginine (14% of total arginyl residues).
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D008099:Liver / Q000648:ultrastructure; D008970:Molecular Weight; D009698:Nucleoproteins / Q000302:isolation & purification*; D011506:Proteins / Q000032:analysis; D051381:Rats; D012261:Ribonucleoproteins / Q000302:isolation & purification*
D000596:Amino Acids; D009698:Nucleoproteins; D011506:Proteins; D012261:Ribonucleoproteins