[The determination of the red cell isoenzymes 6-phosphogluconate dehydrogenase (E.C.1.1.1.44) and acid phosphatase (E.C.3.1.3.2) by means of agarosegel thinlayer electrophoresis (author's transl)].
Methods for the determination of the red cell isoenzymes 6-PGD and acP by means of agarosegel thinlayer electrophoresis are referred. Using these relatively simple techniques, results can be obtained after at least two hours. The quality of separation in both systems is higher than that obtained after starch gel electrophoresis.
D000135:Acid Phosphatase / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification*; D008722:Methods; D010734:Phosphogluconate Dehydrogenase / Q000302:isolation & purification*
D007527:Isoenzymes; D010734:Phosphogluconate Dehydrogenase; D000135:Acid Phosphatase
Die Darstellung der Systeme 6-Phosphogluconat-Dehydrogenase (E.C.1.1.1.44) und der sauren Erythrozyten-Phosphatase (E.C.3.1.3.2) in der Agarosegel-Dünnschicht-Elektrophorese.