Purification of two forms of the associated 3-dehydroquinate hydro-lyase and shikimate:NADP+ oxidoreductase in Phaseolus mungo seedlings.
Two associated enzymes, 3-dehydroquinate hydro-lyase (EC 4.2.1.10) and shikimate:NADP+ oxidoreductase (EC 1.1.1.25), have been purified from Phaseolus mungo seedlings. These enzymes were purified 6900- and 9700-fold, respectively, but they were not separable. Moreover, two activity bands of the shikimate:NADP+ oxidoreductase were detected after polyacrylamide gel electrophoresis and the two peaks also have 3-dehydroquinate hydro-lyase activity. The two forms of the associated enzymes showed only small differences in molecular weight, Km value, pH optimum and the responses to some inhibitors.
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D006836:Hydro-Lyases / Q000302:isolation & purification* / Q000378:metabolism; D007527:Isoenzymes / Q000302:isolation & purification; D007700:Kinetics; D008970:Molecular Weight; D010944:Plants / Q000201:enzymology*; D011801:Quinic Acid / Q000031:analogs & derivatives; D012765:Shikimic Acid
D007527:Isoenzymes; D011801:Quinic Acid; D012765:Shikimic Acid; D000429:Alcohol Oxidoreductases; D006836:Hydro-Lyases