Studies on lectins. XXXV. Water-soluble O-glycosyl polyacrylamide derivatives for specific precipitation of lectins.
By copolymerization of acrylamide and allyl glycosides of various sugars, O-glycosyl derivatives of polyacrylamide copolymers were prepared. The sugar content of the copolymers can be varied in the range 0--40%, their sedimentation coefficient shows the vales of 2.5-5.7 S; the molecular weight of an O-alpha-D-mannopyranosyl polyacrylamide copolymer (29% mannose, so20,w = 2.9 S) was estimated as 44 500. Copolymers with incorporated glycosyl residues interacting specifically with lectins yield precipitates with them upon immunodiffusion in cellulose acetate. The quantitative precipitin curves obtained with these copolymers are similar to those produced by quantitative precipitation of lectins with natural polysaccharides. The copolymers may serve as model substances of natural polysaccharides.
D000178:Acrylamides; D011232:Chemical Precipitation; D003208:Concanavalin A; D006027:Glycosides; D037102:Lectins / Q000302:isolation & purification; D008970:Molecular Weight; D011108:Polymers; D012995:Solubility; D014461:Ultracentrifugation
D000178:Acrylamides; D006027:Glycosides; D037102:Lectins; D011108:Polymers; D003208:Concanavalin A