[Human fetal antehypophysis in vitro. II. Immunocytological study and radioimmunoassay of LH and FSH (author's transl)].
Human fetal antehypophysis (16 males, 16 females and 4 unknown sex) were cultivated during several weeks. By immunocytochemistry LH gonadotroph cells were determined with anti-hTSH and anti-pLH serum. The release in vitro of LH and FSH was studied by radioimmunoassay. At the first medium change, the quantity of LH and FSH release was related to the gestational age and sex. A rapid decline of both LH and FSH occured over the 10 first days. There after, a basal release of LH was maintained during several months; the release of FSH was generally maintained at the lower limit of the assay. After 1 month in vitro, the level of LH could not be related to the sex. Release of LH was stimulated by synthetic LRF. A significant increase was observed independently of the sex and age of the fetuses studied.
D046508:Culture Techniques; D005260:Female; D005333:Fetus; D005640:Follicle Stimulating Hormone / Q000032:analysis*; D005865:Gestational Age; D006801:Humans; D007120:Immunochemistry; D007986:Luteinizing Hormone / Q000032:analysis*; D008297:Male; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000196:embryology*; D011247:Pregnancy; D011863:Radioimmunoassay; D012737:Sex Factors
D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
L'antéhypophyse foetale humaine en culture. II. Etude immunocytologique et radioimmunologique de LH et FSH.