Hepatotoxicity of salicylates in monolayer cell cultures.
The influence of graded doses of sodium salicylate on rat liver cells cultured in monolayers was assessed by measuring lactic dehydrogenase activity in a culture media after incubation. Morphological alterations were studied by electron microscopy. The influence of different albumin concentrations in the media on toxicity was also evaluated. Lactic dehydrogenase concentrations rose with increasing doses of salicylate up to 40 mg per dl. High concentrations of albumin were associated with reduced salicylate toxicity. These findings suggest that salicylate-induced hepatic injury is dose related and my be influenced by serum albumin levels
D000418:Albumins / Q000378:metabolism; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology*; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051381:Rats; D012459:Salicylates / Q000633:toxicity*; D012709:Serum Albumin / Q000378:metabolism
D000418:Albumins; D003470:Culture Media; D012459:Salicylates; D012709:Serum Albumin; D007770:L-Lactate Dehydrogenase