Morphologic effect of hydantoin drugs on mitosis and microtubules of cultured human lymphocytes.
A new class of mitotic inhibitors is described. Diphenylhydantoin and 5-(4-hydroxyphenyl)-5-phenylhydantoin (HPPH), its metabolite, increase the mitotic index of cultured human lymphocytes without affecting the second resting phase (G2). Mitotic inhibition by these compounds is time dependent, and not a property of hydantoins in general. HPPH proved to be more potent than diphenylhydantoin. Electron micrographs of HPPH-treated human lymphocytes showed changes in 45% of the cells in metaphase: a decrease in microtubules or the appearance of 100 A microfilaments. These changes resemble those induced by colchicine.
D002478:Cells, Cultured; D006801:Humans; D006827:Hydantoins / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D008677:Metaphase / Q000187:drug effects; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects*; D008938:Mitosis / Q000187:drug effects*
D006827:Hydantoins