Assessment by microflow fluorometry of the purity of interstitial cell suspensions from the rat testis.
The technique of microflow fluorometry (MFF) was used to identify the proportion of haploid cells (from the tubules) in interstitial cell suspensions. The MFF estimates of the degree of contamination by tubular elements correlated well with the numbers of cells with Leydig cell morphology and those staining positively for 3beta-hydroxysteroid dehydrogenase.
D000818:Animals; D002469:Cell Separation; D005470:Fluorometry; D007985:Leydig Cells; D008297:Male; D051381:Rats