Direct radioimmunoassay of plasma aldosterone in normal subjects.
A direct radioimmunoassay of plasma aldosterone concentration using an antibody of high specificity is described and compared to a previous chromatographic radioimmunoassay. A significant correlation was found between the plasma aldosterone concentration determined by this direct radioimmunoassay and by a radioimmunoassay including paper chromatography in 38 plasma samples obtained from patients with hypertension of various etiology. This direct radioimmunoassay of plasma aldosterone concentration is simpler and less time-consuming. A significant and similar correlation between plasma aldosterone concentration and plasma renin activity (active renin) and renin concentration (total renin) was found in normal subjects on a regular and a low sodium intake. Plasma aldosterone concentration in normal subjects was significantly and negatively related to age and to the 24-h urinary sodium excretion.
D000328:Adult; D000367:Age Factors; D000368:Aged; D000450:Aldosterone / Q000097:blood*; D002854:Chromatography, Paper; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D008875:Middle Aged; D011863:Radioimmunoassay; D012083:Renin / Q000097:blood; D012964:Sodium / Q000494:pharmacology
D000450:Aldosterone; D012964:Sodium; D012083:Renin