Liquid scintillation vial for radiometric assay of lymphocyte carbohydrate metabolism in response to mitogens.
We have demonstrated that mitogens--i.e., PHA and Con.A--stimulate lymphocyte carbohydrate metabolism using a liquid-scintillation vial with conventional liquid-scintillation detectors. The results showed that this enclosed system can be useful for development of rapid in vitro tests of lymphocytes immune responsiveness, as well as for radiometric detection of bacterial growth in various gaseous atmospheres.
D050260:Carbohydrate Metabolism; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology*; D008214:Lymphocytes / Q000378:metabolism*; D010084:Oxidation-Reduction; D012588:Scintillation Counting / Q000295:instrumentation*
D002250:Carbon Radioisotopes; D037102:Lectins; D003208:Concanavalin A