[Duration of the mitotic cyle of mouse liver cells at different stages of carcinogenesis induced by ortho-aminoazotoluene].
Orthoaminoazotoluol was administered to mice for a period of 9 months. The duration of the G2-and S-periods of the mitotic cycle determined by the autoradiographic method, and mitotic duration--by the stathmokinetics technique with the use of colchicine in the intact liver, the cells of the adenoma nodes, and primary hepatomas. The duration of the S-period of the intact hepatocytes and adenoma nodes proved to be equal (13.8 hours, respectively). As to the primary hepatoma cells--it decreased to 12.8 hours. The duration of the G2-period showed no essential change during the carcinogenesis, and was equal to 2.2--2.7 hours. An average mitotic duration in the course of 24 hours was also constant and was approximately equal to 1 hour. A rise in the number of mitoses and PNA-synthesizing cells in the hepatomas occurred as a result of entering in mitosis and the S-period of a greater number of cells, but not as a result to prolongation of the duration of the S-and M-periods of the mitotic cycle.
D000818:Animals; D001345:Autoradiography; D001391:Azo Compounds; D008099:Liver / Q000187:drug effects*; D008113:Liver Neoplasms / Q000139:chemically induced*; D051379:Mice; D008938:Mitosis / Q000187:drug effects*; D009374:Neoplasms, Experimental / Q000139:chemically induced; D013997:Time Factors; D009762:o-Aminoazotoluene
D001391:Azo Compounds; D009762:o-Aminoazotoluene
Prodolzhitel'nost' mitoticheskogo tsikla kletok pecheni mysheĭ na razlichnykh stadiiakh kantserogeneza, indutsirovannogo ortoaminoazotoluolom.