A theoretical analysis of the use of zonal gel filtration in the detection and purification of protein-ligand complexes.
A simple model of zonal gel filtration is analysed numerically to establish semiquantitatively how the capacity of a protein-ligand complex to survive gel filtration intact depends on several experimental variables and on the kinetic characteristics of the complex. The design of gel-filtration experiments aimed at detecting and partially purifying binding proteins is discussed.
D002850:Chromatography, Gel; D007700:Kinetics; D008024:Ligands / Q000032:analysis*; D008956:Models, Chemical; D011506:Proteins / Q000032:analysis*
D008024:Ligands; D011506:Proteins