[Changes in lactate dehydrogenase isoforms in the process of oncogenesis].
Isoenzymes of lactate dehydrogenase were studied by disc-electrophoresis in polyacrylamide gel, and in the clinic--in 1% agar gel. Oncovirus A12 invasion of the culture of rat embryo fibroblasts (REF) was found to result in the increased percentage of the cathode fractions activity (LDG-4 and LD-5) and in the disappearance of LDG-1 yet during the first day of the experiment prior to hypoxia and enhanced proliferation, i. e. it is most likely to be primary. In the homogenates of cancerous tumor and large intestine polyps of man also a reliable increase of the cathode and a decrease or disappearance of the anode fractions accur. A correlation of the experimental and clinical data allowed a suggestion to be made that LDG isoenzymes changes are genetically conditioned and play an important role in the process of oncogenesis, providing conditions for the increased intensity of glycolysis and proliferation.
D000260:Adenoviruses, Human; D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003593:Cytoplasm / Q000201:enzymology; D004622:Embryo, Mammalian / Q000166:cytology; D004789:Enzyme Activation; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007414:Intestinal Neoplasms / Q000201:enzymology; D007417:Intestinal Polyps / Q000201:enzymology; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D009369:Neoplasms / Q000209:etiology*; D009858:Oncogenic Viruses; D051381:Rats; D013997:Time Factors
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase
Izmeneniia izoform laktatdegidrogenazy v protsesse onkogeneza.