Separation of urinary ultraviolet-absorbing metabolites by high-pressure liquid chromatography using a commercially available analytical unit.
A high-pressure liquid chromatographic system for the separation of UV-absorbing and/or fluorescent urinary metabolites is presented. Thirty to forty UV-absorbing chromatographic peaks are obtained from a 200-microliters urine sample using a 100 cm x 2 mm column filled with a total porous ion-exchanger. The reproductibility of the separation has been studied and eighteen important substances have been located.
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D008875:Middle Aged; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014556:Urine / Q000032:analysis*