The effect of D-penicillamine on mitogen-induced human lymphocyte proliferation: synergistic inhibition by D-penicillamine and copper salts.
The effect of D-penicillamine on in vitro mitogen-triggered human peripheral blood lymphocyte DNA synthesis was examined. D-penicillamine caused a modest degree of inhibition of responsiveness. Since D-penicillamine avidly chelates copper, a possible additive effect of D-penicillamine and copper salts on mitogen-induced lymphocyte proliferation was investigated. The addition of various copper salts to D-penicillamine-containing cultures resulted in a marked augmentation in the degree of inhibition observed. Specificity for the synergy between D-penicillamine and copper was suggested by the failure of iron, zinc, or gold salts to augment the inhibition produced by D-penicillamine alone. However, a number of other thiols, but not disulfides, could substitute for D-penicillamine in inhibiting mitogen responsiveness in the presence of copper salts. Preincubation of peripheral blood mononuclear cells with D-penicillamine and CuSO4 resulted in diminished mitogen responsiveness on subsequent culture in the absence of the inhibitors. Data are presented to indicate that preincubation with D-penicillamine and CuSO4 directly affected the ability of the T lymphocytes to respond to mitogens but did not alter the capacity of the monocytes to act as accessory cells in this response.
D000085:Acetates / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology*; D006046:Gold / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008934:Mitogens / Q000494:pharmacology*; D010396:Penicillamine / Q000494:pharmacology*; D013431:Sulfates / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013997:Time Factors
D000085:Acetates; D002712:Chlorides; D008934:Mitogens; D013431:Sulfates; D013438:Sulfhydryl Compounds; D006046:Gold; D003300:Copper; D010396:Penicillamine