Studies on substrate specificity of X-prolyl dipeptidyl-aminopeptidase during new chromogenic substrates, X-Y-p-nitroanilides.
Substrate specificity of X-prolyl dipeptidyl-aminopeptidase (dipeptidyl aminopeptidase IV) was examined by using newly synthesized 8 chromogenic substrates, X-Y-p-nitroanilides. Homogeneous enzyme from human submaxillary gland hydrolyzed glycylproline p-nitroanilide almost specifically, except alanylalanine p-nitroanilide which had 11% activity.
D000813:Anilides; D004151:Dipeptides; D010450:Endopeptidases / Q000378:metabolism*; D006801:Humans; D009574:Nitro Compounds; D013363:Submandibular Gland / Q000201:enzymology; D013379:Substrate Specificity
D000813:Anilides; D004151:Dipeptides; D009574:Nitro Compounds; D010450:Endopeptidases