Kinetics of phagocytosis of Chlamydia psittaci by mouse fibroblasts (L cells): separation of the attachment and ingestion stages.
The kinetics of phagocytosis of Chlamydia psittaci (6BC) by monolayers of mouse fibroblasts (L cells) was studied with an assay that distinguished between the attachment and ingestion phases of phagocytosis. At multiplicities of 10 and 100 50% infectious doses (ID50) per L cell, virtually all of the inoculated C. psittaci had been attached and ingested after 60 min at 37 degrees C. At multiplicities of 500 to 5,000 ID50 per L cell, the initial rates of attachment and ingestion of C. psittaci to L cells increased with the multiplicity of infection, but phagocytosis stopped even though many chlamydial cells remained free in suspension and readily available for attachment to the host-cell monolayers. Phagocytosis probably ceased because the L cells were injured when they took up large numbers of chlamydial cells. This injury prevented direct determination of the number of potential binding sites for C. psittaci on each L cell. However, this number is large enough to make the rates of chlamydial attachment and ingestion predominantly dependent on the multiplicity of infection.
D001665:Binding Sites; D002691:Chlamydophila psittaci / Q000254:growth & development / Q000276:immunology*; D007700:Kinetics; D007739:L Cells / Q000276:immunology* / Q000648:ultrastructure; D010587:Phagocytosis