An accurate, sensitive and reproducible method for the colorimetric estimation of free fatty acids in plasma.
A sensitive and reproducible method permitting the estimation of 0,030-3,000 mM free fatty acids (FFA) in plasma with a standard error of 0,006 5 was developed. The method is based on the extraction of phosphate-buffered plasma with a chloroform-heptane-methanol mixture, formation of the cobalt complex and on the subsequent determination of the metal with 1-nitroso-2-naphthol. The method was used to obtain normal values (mean 0,547 mM range 0,147-1,197 mM) for Merino wethers fed lucerne hay.
D000818:Animals; D003124:Colorimetry / Q000379:methods*; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D008297:Male; D010713:Phosphatidylcholines / Q000097:blood; D012756:Sheep / Q000097:blood
D005230:Fatty Acids, Nonesterified; D010713:Phosphatidylcholines