Amino acid sequence of the basic trypsin inhibitor from bovine splenic capsule. A new fluorescent reagent for protein sequence analysis.
The amino acid sequences of tryptic peptides from the performic acid-oxidized trypsin inhibitor were determined. The degradation was performed with a new reagent, 3-isothiocyanato-4-methoxy-4'-nitrostilbene, and compared with the dansyl-Edman technique. The amino acid sequence of the trypsin inhibitor from bovine splenic capsule was found to be the same as that of the basic trypsin inhibitor from bovine pancreas, established by Kress & Laskowski (J. Biol. Chem. 1967, 242, 4925-4928).
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D005453:Fluorescence; D007202:Indicators and Reagents; D017879:Isothiocyanates; D013154:Spleen / Q000378:metabolism; D013267:Stilbenes; D013861:Thiocyanates; D014361:Trypsin Inhibitors / Q000032:analysis*
C016528:3-isothiocyanato-4-methoxy-4'-nitrostilbene; D007202:Indicators and Reagents; D017879:Isothiocyanates; D013267:Stilbenes; D013861:Thiocyanates; D014361:Trypsin Inhibitors