Asynchronous globin chain synthesis in rabbit reticulocyte lystates induced by hemin-controlled repressor.
To define further the role of hemin-controlled repressor (HCR) in globin synthesis, we studied its effect on the synthesis of individual globin chains in a rabbit reticulocyte lysate cell-free system. In the presence of HCR there was a marked globin chain imbalance, resulting in a lowered alpha/beta ratio. These findings in vitro may have relevance to certain clinical heme deficiency states in which a similar globin chain imbalance has been observed.
D000818:Animals; D005260:Female; D005914:Globins / Q000096:biosynthesis*; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes; D013997:Time Factors
D006418:Heme; D006427:Hemin; D005914:Globins; D007930:Leucine