Differential effect of ethanol ingestion on the protein synthetic activities of free and membrane-bound ribosomes from liver of the weanling rat.
The influence of chronic ethanol ingestion on the protein synthetic activities of free and membrane-bound ribosomes was studied in vitro in the liver of young rat. Ethanol administration caused about 120% increase in the amino acid incorporative activity of free ribosomes. In contrast, membrane-bound ribosomes from the livers of ethanol-fed animals exhibited a 25% decline in their protein synthetic activity. This decline in the activity of membrane-bound ribosomes was abolished after dissolution of membranes by Triton X-100. Evidence was obtained suggesting that the observed alterations in protein synthesis due to ethanol intake are attributable to changes in pH5 enzyme fraction, altered amount of ribosome-associated mRNA and a damage to the endoplasmic reticulum membranes.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008566:Membranes / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D012269:Ribosomal Proteins / Q000096:biosynthesis*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*
D000596:Amino Acids; D012269:Ribosomal Proteins; D000431:Ethanol; D011092:Polyethylene Glycols