Development of haematopoietic colonies on the macrophage layer formed in the peritoneal cavity of S1/S1d mice.
The colony-forming ability of haematopoietic cells was examined on the macrophage layer formed in the peritoneal cavity of S1/S1d mice. The bone marrow cells of the congenic +/+ mice formed many macroscopic colonies on the macrophage layer of the S1/S1d mice although they did not form macroscopic colonies in the spleens of the same S1/S1d recipients. The size and the differentiation pattern of colonies on the macrophage layer of the S1/S1d mice were comparable to those of the colonies on the macrophage layer of the +/+ mice. There are two possible explanations for these results: (a) The microenvironmental defect of the S1/S1d mice has a more prominent effect on the development of spleen colonies than that of macrophage-layer colonies because 'Steel' locus may not be expressed significantly in the peritoneal macrophages or (b) because the cells that make colonies on the macrophage layer may be more differentiated cells than the multipotential stem cells that make colonies in the spleen.
D000818:Animals; D002999:Clone Cells; D004920:Erythropoiesis; D005796:Genes; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008264:Macrophages; D051379:Mice; D009154:Mutation; D010529:Peritoneal Cavity / Q000166:cytology; D013154:Spleen