Large-scale purification of hepatitis B surface antigen using affinity chromatography.
Large-scale purification of hepatitis B surface antigen, applicable to the preparation of potential vaccines for prevention of hepatitis B, is described. The method involves the following steps: precipitation of serum with polyethylene glycol 6000, affinity chromatography on concanavalin A-Sepharose and on omega-aminononyl-Sepharose, and isopycnic centrifugation.
D002500:Centrifugation, Isopycnic; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
D006514:Hepatitis B Surface Antigens; D003208:Concanavalin A; D012685:Sepharose