Preparative immunoabsorption electrophoresis.
A method for separating a component from a mixture of antigens is described. The component which may be a virus or subfraction of a virus, is isolated by driving the mixture by electrophoresis through a gel containing precipitating antibodies directed against the unwanted components. The method is illustrated by the isolation of hepatitis B antigen from whole serum and by the separation of wild cucumber mosaic virus from a strain of tobacco mosaic virus.
D000042:Absorption; D000818:Animals; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification; D006801:Humans; D007122:Immunoelectrophoresis / Q000295:instrumentation / Q000379:methods*; D009029:Mosaic Viruses / Q000302:isolation & purification / Q000648:ultrastructure; D011817:Rabbits
D006514:Hepatitis B Surface Antigens