Regulation of the beta-1,3-glucanase system in Penicillium italicum: glucose repression of the various enzymes.
The microscopic fungus Penicillium italicum when grown in a synthetic liquid medium produced at least three enzymes with beta-1,3-glucanase activity which were separated by diethylaminoethyl-Sephadex column chromatography. These were named beta-1,3-glucanases I, II, and III respective to their order of elution from the column. A tentative characterization of these three enzymes indicated that they have different modes of action; the first one is an endoglucanase, the second is an exoglucanase, and the third probably has both mechanisms of action. Glucose had a repressive effect on all three enzymes. Only small amounts of beta-1,3-glucanases II and III were present in the cells when they were actively growing in the presence of this sugar. However, when the cells were transferred to a medium low in glucose, a significant increase in the specific activity of beta-1,3-glucanase took place; this was due in part to a much more active production of beta-1,3-glucanases II and III and in part to the appearance of beta-1,3-glucanase I, which could only be detected after more than 12 h of incubation in this medium. The results are discussed in the context of possible beta-1,3-glucanase functions in the fungal cells.
D002852:Chromatography, Ion Exchange; D004794:Enzyme Repression; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007700:Kinetics; D010407:Penicillium / Q000201:enzymology*
D006026:Glycoside Hydrolases; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D005947:Glucose