The relation between sodium transport and oxygen consumption in isolated perfused rat kidney.
A non-filtering isolated perfused rat kidney model was developed to determine renal oxygen consumption in the absence of tubular reabsorption. The oxygen consumption in the absence of filtration was 3.1 +/- 0.8 micron of O2/min/g. This "basal" value was substantially higher than that of 2.0 micron of O2/min/g calculated by extrapolation of a plot of sodium reabsorption vs. oxygen consumption in filtering kidneys. It was also found that ouabain 10(-4) M decreased oxygen consumption in non-filtering kidneys and acetazolamide increased it in a dose dependent fashion. Furosemide did not change "basal" oxygen consumption. In filtering kidneys, the ratio of sodium reabsorbed to oxygen consumed averaged 36.8 muEq of Na/mumole of O2. It is concluded that basal oxygen consumption is not a fixed quantity and can be changed by experimental manipulations thereby changing the relation between transport activity and oxygen consumption.
D000086:Acetazolamide / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D005665:Furosemide / Q000494:pharmacology; D066298:In Vitro Techniques; D007444:Inulin; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D010477:Perfusion; D051381:Rats; D012964:Sodium / Q000378:metabolism*
D010042:Ouabain; D005665:Furosemide; D007444:Inulin; D012964:Sodium; D000086:Acetazolamide