The linkage of sugar phosphate polymer to peptidoglycan in walls of Micrococcus sp. 2102.
1. Protein-free walls of Micrococcus sp. 2102 contain peptidoglycan, poly-(N-acetylglucosamine 1-phosphate) and small amounts of glycerol phosphate. 2. After destruction of the poly-(N-acetylglucosamine 1-phosphate) with periodate, the glycerol phosphate remains attached to the wall, but can be removed by controlled alkaline hydrolysis. The homogeneous product comprises a chain of three glycerol phosphates and an additional phosphate residue. 3. The poly-(N-acetylglucosamine 1-phosphate) is attached through its terminal phosphate to one end of the tri(glycerol phosphate). 4. The other end of the glycerol phosphate trimer is attached through its terminal phosphate to the 3-or 4-position of an N-acetylglucosamine. It is concluded that the sequence of residues in the sugar 1-phosphate polymer-peptidoglycan complex is: (N-acetylglucosamine 1-phosphate)24-(glycerol phosphate)3-N-acetylglucosamine 1-phosphate-muramic acid (in peptidoglycan). Thus in this organism the phosphorylated wall polymer is attached to the peptidoglycan of the wall through a linkage unit comprising a chain of three glycerol phosphate residues and an N-acetylglucosamine 1-phosphate, similar to or identical with the linkage unit in Staphylococcus aureus H.
D002473:Cell Wall / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D005994:Glycerophosphates / Q000302:isolation & purification; D006868:Hydrolysis; D008837:Micrococcus / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism; D011485:Protein Binding; D013403:Sugar Phosphates / Q000378:metabolism*
D005994:Glycerophosphates; D010457:Peptidoglycan; D011108:Polymers; D013403:Sugar Phosphates