In situ assay of poxvirus-induced and virion-contained deoxyribonucleases in DNA polyacrylamide gels.
Cowpox virus and vaccinia virus WR specific DNases were identified by an in situ assay using DNA-containing acrylamide gels. A DNase present in the isolated virions of strain cowpox and vaccinia WR was identified. Its properties as determined by the in situ assay resembled those of the well-characterized DNase V1. The assay was also used to follow the time course of 'acid' DNase induction by cowpox virus in primary chick embryo fibroblasts.
D002478:Cells, Cultured; D003851:Deoxyribonucleases / Q000032:analysis* / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D007700:Kinetics; D014616:Vaccinia virus / Q000201:enzymology*; D014771:Virion / Q000201:enzymology*
D003851:Deoxyribonucleases