[Biosynthesis of the labelled rifamycin B in the presence of different 14C- and 3H-precursors].
The results of studying the effectiveness of incorporation of the label from different 14C- and 3H-precursors into the molecule of rifamicin B during its biosynthesis are presented. The regularities of the label incorporation into the antibiotic composition as dependent on the time of the precursor addition were investigated. A radiochemically pure preparation of 14C-rifamicin B with specific radioactivity of 3 mcurie/mg was obtained.
D000085:Acetates / Q000378:metabolism; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism; D011422:Propionates / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism*; D012294:Rifamycins / Q000096:biosynthesis*; D012588:Scintillation Counting; D013997:Time Factors; D014316:Tritium; D014466:Ultraviolet Rays
D000085:Acetates; D002250:Carbon Radioisotopes; D003470:Culture Media; D011422:Propionates; D011498:Protein Precursors; D012294:Rifamycins; D014316:Tritium; D005947:Glucose
Biosintez mechenogo rifamitsina B v prisutstvii razlichnykh 14C- i 3H-predshestvennikov.