Establishment of Marek's disease lymphoblastoid cell lines from transplantable versus primary lymphomas.
Six new Marek's disease (MD) lymphoblastoid cell lines were established in vitro by cultivation in a medium containing 2-mercaptoethanol (2-ME). Attempts using primary lymphoma cells were generally unsuccessful; only one of 28 lymphomas yielded a cell line and that one came from an experimentally immunosuppressed chicken. In contrast, two of seven low-passage, and two of two established MD transplantable lymphomas grew readily in vitro. A sixth line was obtained using buffy coat cells from a leukemic chicken. It was concluded that the use of transplantable tumor cells and a medium containing 2-ME provided a combination highly suited to the establishment of cell lines from MD.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002645:Chickens; D003470:Culture Media; D007106:Immune Sera; D008223:Lymphoma; D008380:Marek Disease; D008623:Mercaptoethanol; D008722:Methods; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental
D000956:Antigens, Viral; D003470:Culture Media; D007106:Immune Sera; D008623:Mercaptoethanol