Ribonuclease H activity in cultured plant cells.
Ribonuclease H (RNAase H) was extracted from cultured plant cells, strain GD-2 and characterized. RNAase H activity in logarithmical growing cells is much higher than that of stationary cells, and the response of RNAase H activity was very similar to that of DNA polymerase after culture. The activities of RNAase, DNAase, phosphodiesterase and alkaline phosphatase decrease parallel with the increase in growth, and increase to stationary phase, contrasting with those of DNA polymerase and RNAase H.
D002455:Cell Division; D002478:Cells, Cultured; D007700:Kinetics; D010944:Plants / Q000201:enzymology*; D012260:Ribonucleases / Q000378:metabolism*
D012260:Ribonucleases