Determination of sodium phenobarbital in animal chow by high-pressure liquid chromatography.
An analytical procedure is described for determining residues of sodium phenobarbital in animal chow at levels as low as 0.14 ppm. The methanol extract is subjected to a liquid-liquid cleanup at pH 13 and 1, further cleaned up on a silica gel column and assayed by high-pressure liquid chromatography by using an ultraviolet absorption detector at 210 nm. Data concerning extraction efficiency, partition values and stability of the chemical in animal chow are also presented.
D000821:Animal Feed / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008832:Microchemistry; D010634:Phenobarbital / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D010634:Phenobarbital