GLC determination of plasma concentrations of gamma-oxo metabolite of phenylbutazone.
A quantitative method for the gamma-oxo metabolite of phenylbutazone from plasma is described. The procedure involved an ethylene dichloride extraction of acidified plasma to which an internal standard, acenocoumarol, had been added. The extracted gamma-oxo metabolite and the internal standard were methylated and analyzed by GLC. Determination of 0.25 microgram of gamma-oxo metabolite/ml with a relative standard deviation of 6.5% was accomplished.
D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D010653:Phenylbutazone / Q000031:analogs & derivatives* / Q000097:blood; D013997:Time Factors
D010653:Phenylbutazone