Erythroid progenitors circulating in the blood of adult individuals produce fetal hemoglobin in culture.
Erythroid colonies, raised from erythroid stem cells circulating in the peripheral blood of normal adult individuals, synthesize considerable amounts of fetal hemoglobin. In cultures from persons with sickling disorders, amounts of hemoglobin F that are known to inhibit sickling in vivo are produced. The results provide evidence that primitive erythroid progenitors are able to express the hemoglobin F production program and that cultures of mononuclear cells of the adult blood can be used to investigate the mechanisms involved in regulation of gamma-globin gene switching.
D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood; D002454:Cell Differentiation; D002478:Cells, Cultured; D005319:Fetal Hemoglobin / Q000096:biosynthesis*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006441:Hemoglobin A / Q000096:biosynthesis; D006451:Hemoglobin, Sickle / Q000096:biosynthesis; D006801:Humans; D012156:Reticulocytes / Q000378:metabolism; D013789:Thalassemia / Q000097:blood
D006451:Hemoglobin, Sickle; D006441:Hemoglobin A; D005319:Fetal Hemoglobin