[Fast and accurate method for theophylline analysis in preterm babies using gas chromatography and nitrogen selective detector (NFID) (author's transl)].
The combined utilization of (a) a weak polar OV-1 phase, (b) an on-column methylation using a methylating agent commercially available, (c) a nitrogen selective detector (NFID) and (d) a small volume of sample, allows the development of a fast (less than 20 min) and accurate method for theophylline analysis in preterm babies. With 50 microliter of capillary whole blood sample, its limit of sensitivity was 0.5 microgram/ml of theophylline. This method is most desirable for theophylline monitoring in treating apnea of premature infants.
D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007231:Infant, Newborn; D007235:Infant, Premature, Diseases / Q000378:metabolism*; D008722:Methods; D013806:Theophylline / Q000032:analysis*
D013806:Theophylline
Méthode de chromatographie en phase gazeuse-NFID pour la détermination rapide de la théophylline sanguine chez les enfants prématures.