Quantitation of 25-hydroxycholecalciferol in human serum by high-pressure liquid chromatography.
An HPLC method for assaying 25(OH)D3 in extracts of human serum is described. The method involves extraction of serum with methanol-dichloromethane and chromatography on a column of Sephadex LH-20 prior to HPLC with quantitation by UV absorbance. Comparison with a CBP assay for 25(OH)D3 showed no difference between the two methods.
D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006887:Hydroxycholecalciferols / Q000097:blood*; D011485:Protein Binding
D006887:Hydroxycholecalciferols