The acidic amino acid transport system of the baby hamster kidney cell line BHK21-C13.
The uptake of L-glutamate into BHK21-C13 cells in culture has been studied. This amino acid appears to be transported via a relatively high affinity, low capacity, Na+-dependent transport system capable of the rapid accumulation of substrate amino acids. Kinetic studies of the inhibition of L-glutamate uptake has provided information as to the substrate and the molecular configuration required for transport via the glutamate transport system. This system exhibited marked substrate specificity and was only capable of transporting L-glutamate and aspartate and certain closely related acidic amino acid analogues.
D001224:Aspartic Acid / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D003544:Cysteic Acid / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D007700:Kinetics; D010042:Ouabain / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D005971:Glutamates; D013439:Sulfhydryl Reagents; D001224:Aspartic Acid; D010042:Ouabain; D012964:Sodium; D003544:Cysteic Acid