[Effect of the protein product of phage f1, gene 5, on heat denaturation of synthetic polynucleotides].
The influence of phage f1 gene 5 protein on melting of the synthetic polynucleotides has been investigated, using UV-spectroscopy. In our experiments we have varied the proteins concentration. It has been shown, that the protein lowers the melting temperature of the studied polynucleotides (d/A--Tn dAndTn, rAndTn, rAn.r n, dAn.rn). The melting temperatures and the shapes of melting curves of various polynucleotides differ when the same protein concentrations are used. We have shown that the protein binds to the double-stranded polynucleotides, containing ribo-ribo-, deoxyribo-ribo-chains. The difference in melting temperatures and shapes of melting curves was explained using the data about the differences in the secondary structure of these polynucleotides. Only for d/A-Tn renaturation was observed after sample cooling. It may reflect the single-stranded hairpin structure of this polynucleotide.
D001435:Bacteriophages / Q000235:genetics*; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation / Q000187:drug effects*; D011063:Poly A-U; D011067:Poly dA-dT; D011119:Polynucleotides; D014764:Viral Proteins / Q000235:genetics / Q000494:pharmacology*
D011119:Polynucleotides; D014764:Viral Proteins; D011063:Poly A-U; D011067:Poly dA-dT
Vliianie belka--produkta gena 5 faga f1--na teplovuiu denaturatsiiu sinteticheskikh polinukleotidov.