Abnormal electrophoretic pattern of albumin in diabetic sera.
Human sera collected from normal subjects and diabetic patients were electrophoresed in an urea-containing gel. The albumin fraction separated into several bands. In normal fasting serum the fast-migrating Bands 1 and 2 were observed and the slower-migrating Bands 4 and 5 did not appear. After glucose administration Band 1 disappeared and Bands 4 and 5 appeared for the first time. In diabetic serum Bands 4 and 5 did not appear before or after glucose administration and this abnormality resembles the pattern in C57BL/KsJ-db/db mice but not C57BL/6J-ob/ob mice.
D003920:Diabetes Mellitus / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005951:Glucose Tolerance Test; D006801:Humans; D012709:Serum Albumin / Q000032:analysis*
D005230:Fatty Acids, Nonesterified; D012709:Serum Albumin