Decreased serum responsiveness by primary monolayer cultures of preneoplastic and neoplastic mammary epithelial cells.
Monolayer cultures of normal, preneoplastic and neoplastic murine mammary epithelial cells were exposed to various types of mammalian serum. A progressive decline in levels of thymidine incorporation together with a change in the ordering of sera which stimulates optimal incorporation was observed in the transformed cells.
D000818:Animals; D001769:Blood; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D051379:Mice; D011230:Precancerous Conditions / Q000378:metabolism*; D011817:Rabbits; D012756:Sheep; D013552:Swine; D013936:Thymidine / Q000378:metabolism
D003470:Culture Media; D013936:Thymidine