Stereoconfiguration of phosphatidylglycerol in the membrane of bacteriophage PM2 and in its host, Pseudomonas BAL-31.
Turnover of the acylated and unacylated glycerol moieties of phosphatidylglycerol was examined during infection of Pseudomonas BAL-31 by bacteriophage PM2. No turnover of either glycerol moiety was observed in infected or inunfected cells. The stereochemical configuration of phosphatidylglycerol from both virus and host was determined and proved to be 3-sn-phosphatidyl-1'-sn-glycerol. These results exclude a mechanism of mobilizing lipids for the virus by acylation and deacylation of 3-sn-phosphatidyl-1'-sn-glycerol in the host membrane to form 1-sn-phosphatidyl-3'-sn-glycerol in the membrane of PM2.
D001435:Bacteriophages / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D010715:Phosphatidylglycerols; D011549:Pseudomonas / Q000378:metabolism*; D013237:Stereoisomerism; D014779:Virus Replication
D008563:Membrane Lipids; D010715:Phosphatidylglycerols