Relationship between cell proliferation, chromatin template activity and accumulation of nuclear proteins.
Trypsinization of confluent monolayers of WI-38 cells causes an extensive loss of nuclear proteins. The loss of nuclear proteins is restored only several hours after the cells have been replated at a lower density in 10% serum. When trypsinized fibroblasts are replated at a lower density in 10% serum, there is also a sustained progressive leading to DNA synthesis and cell division. If 0.3% serum is used instead of 10%, there is a modest increase in nuclear template activity, but not accumulation of nuclear proteins and no DNA synthesis or cell division.
D001769:Blood; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts; D009698:Nucleoproteins / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis; D013698:Templates, Genetic; D014357:Trypsin / Q000494:pharmacology
D002843:Chromatin; D003470:Culture Media; D009698:Nucleoproteins; D012313:RNA; D004247:DNA; D014357:Trypsin