Early appearance and mitotic activity of multinucleated giant cells in mice after combined injection of talc and prednisolone acetate. A model for studying rapid histiocytic polykarion formation in vivo.
Combined intraperitoneal injection of talc and particulate prednisolone acetate resulted in the formation of significant numbers of multinucleated giant cells within 48 hours. Neither one of these two agents had a comparable effect when injected alone. Evidence is presented showing that the multinucleated giant cells arose by cell fusion. Synchronous nuclear division could be observed in a considerable proportion of newly formed polykarions. Although the late prophases were usually normal, high proportions of abnormal mitotic figures were observed at later stages of mitosis, particularly in anaphase and telophase. The chromosome abnormalities were so severe that it is highly improbable that mitosis led to successful nuclear multiplication within a given syncytium. Similar to prednisolone acetate, prednisone elicited the formation of multinucleated giant cells when injected with talc. No polykarion formation was observed, however, when prednisolone acetate was substituted for other steroid preparations of similar particle size such as cortexon acetate, cortisone, or testosterone isobutyrate.
D000818:Animals; D002459:Cell Fusion; D002467:Cell Nucleus / Q000187:drug effects; D003348:Cortisone / Q000494:pharmacology; D003900:Desoxycorticosterone / Q000494:pharmacology; D006101:Granuloma, Giant Cell / Q000139:chemically induced / Q000473:pathology*; D006644:Histiocytes / Q000473:pathology*; D051379:Mice; D008813:Mice, Inbred ICR; D008938:Mitosis; D011239:Prednisolone / Q000494:pharmacology*; D011241:Prednisone / Q000494:pharmacology; D013627:Talc / Q000494:pharmacology*; D013739:Testosterone / Q000494:pharmacology
D013627:Talc; D013739:Testosterone; D003900:Desoxycorticosterone; D011239:Prednisolone; D003348:Cortisone; D011241:Prednisone