An immunoglobulin E assay using radiolabelled Fab' and ammonium sulfate.
An immunochemical assay is described in which a radiolabelled antibody fragment, Fab', is bound specifically to immunoglobulin E (IgE), and precipitated with ammonium sulfate. The radioactivity in the precipitate is a measure of the amount of IgE in the sample. Results for six serum samples are compared using the double antibody and ammonium sulfate methods as well as the PRIST.
D000645:Ammonium Sulfate / Q000494:pharmacology*; D000818:Animals; D000918:Antibody Specificity; D006801:Humans; D007073:Immunoglobulin E; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D011485:Protein Binding; D011817:Rabbits
D007457:Iodine Radioisotopes; D007073:Immunoglobulin E; D000645:Ammonium Sulfate