Determination of disaccharides in feces by permethylation and gas chromatography: rapid screening for carbohydrate intolerance in children.
Disaccharides can be identified and determined quantitatively in fecal specimens by permethylation of the components in crude supernatants followed by isothermal gas chromatographic separation on OV-17 columns. The method provides a rapid screening procedure for detecting patients suspected of having a carbohydrate intolerance secondary to intestinal disaccharidase deficiency.
D002239:Carbohydrate Metabolism, Inborn Errors / Q000175:diagnosis*; D002648:Child; D002849:Chromatography, Gas; D004187:Disaccharides / Q000032:analysis*; D005243:Feces / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry; D008722:Methods
D004187:Disaccharides