Sensitive gas chromatographic methods for the determination of vinyl epoxide synthetase activity using trichloroethylene as a model substrate.
Two specific and very sensitive methods for the determination of vinyl epoxide synthetase activity in liver microsomes are described. Trichloroethylene, which is used as a substrate, is converted into trichloroethylene oxide by a hepatic epoxide synthetase. Chloral hydrate, the final rearrangement product of trichloroethylene oxide, is determined by electron-capture gas chromatography, either after derivatization with pentafluorophenylhydrazine or after its conversion into chloroform under alkaline conditions. The kinetic parameters of the epoxidation reaction were determined on rat hepatic microsomal suspensions.
D000818:Animals; D002849:Chromatography, Gas; D004852:Epoxy Compounds; D008862:Microsomes, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D014241:Trichloroethylene; D014753:Vinyl Compounds
D004852:Epoxy Compounds; D014753:Vinyl Compounds; D014241:Trichloroethylene; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; C023289:vinyl epoxide synthetase