Effect of concanavalin A and its succinylated derivative on the oxidative metabolism of guinea pig peritoneal macrophages.
The effect of concanavalin A and its succinylated derivative on the metabolic regulation of guinea pig peritoneal macrophages was observed. The binding of tetravalent concanavalin A to the surface glycoproteins of macrophages caused a marked increase in the rate of oxygen consumption due to the activation of the hexose monophosphate shunt. Divalent succinylated concanavalin A, also induced a similar change in the rate of oxygen metabolism. The metabolic change induced by these two types of lectin was reversibly inhibited by alpha-methyl-D-glucoside, a haptenic inhibitor of these lectins, and was temperature dependent (observed at above 15 degrees C). It is suggested that the binding of these lectins to the surface glycoproteins, and not their cross-linking into caps, is required for the activation of oxygen metabolism of macrophages, and that highly fluid state of the plasma membrane seems to be an essential requirement for the transduction of glycoprotein perturbation on the macrophage surface into cellular interior via transmembrane control mechanism.
D000818:Animals; D002462:Cell Membrane; D003208:Concanavalin A / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006023:Glycoproteins / Q000378:metabolism; D006168:Guinea Pigs; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D010529:Peritoneal Cavity / Q000166:cytology; D011952:Receptors, Concanavalin A / Q000378:metabolism; D013329:Structure-Activity Relationship
D006023:Glycoproteins; D011952:Receptors, Concanavalin A; D003208:Concanavalin A