Isolation of 4.5-dihydroxyisophthalic acid an inhibitor of brain glutamate decarboxylase, produced by a Streptomyces species.
A potent inhibitor of brain glutamate decarboxylase (L-glutamate 1-carboxy-lyase, EC 4.1.1.15) was isolated from cultures of a Streptomyces species, and its structure was found to be 4,5-dihydroxyisophthalic acid. The metabolite inhibited beef brain glutamate decarboxylase 50% at a concentration of 0.12 microgram/ml (0.61 micron). The kinetic analysis indicated that 4,5-dihydroxyisophthalic acid was a competitive inhibitor having a Ki value of 0.18 micron.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D002417:Cattle; D005968:Glutamate Decarboxylase / Q000037:antagonists & inhibitors*; D007700:Kinetics; D010795:Phthalic Acids / Q000302:isolation & purification / Q000494:pharmacology*; D013302:Streptomyces
D010795:Phthalic Acids; C015820:4,5-dihydroxyisophthalic acid; D002262:Carboxy-Lyases; D005968:Glutamate Decarboxylase