Immune response in guinea pigs to two different lipid conjugates of bovine serum albumin.
A lipid conjugate of BSA was made by coupling dodecylamine to the COOH groups of BSA (DA-BSA). Studies on the immunogenicity of this material and one made by coupling dodecanoic anhydride to NH2 groups (D-BSA) demonstrated that cell-mediated immunity could preferentially be generated by lipid conjugation to an antigen as measured by delayed skin reaction, invitro blast transformation, and antibody formation. DA-BSA was found superior by both in vivo and in vitro tests. Both the conjugates retained the ability to suppress delayed type hypersensitivity, but only DA-BSA retained the ability to elicit optimum skin reaction in a sensitized animal and to precipitate anti-BSA antibody.
D000818:Animals; D000917:Antibody Formation; D003520:Cyclophosphamide / Q000494:pharmacology; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular; D007165:Immunosuppression Therapy; D008055:Lipids / Q000494:pharmacology*; D011233:Precipitin Tests; D012710:Serum Albumin, Bovine / Q000494:pharmacology*
D008055:Lipids; D012710:Serum Albumin, Bovine; D003520:Cyclophosphamide