Acetylesterase isoenzymes in rat uterus and placenta.
The occurrence of acetylesterase activity in the uterus and placenta of the rat has been investigated using a general histochemical simultaneous coupling technique after separation on polyacrylamide gradient gels. apart from a complex band associated with serum esterases which was demonstrated in all the tissues studied, several other isoenzyme bands were demonstrable in differing degrees in the yolk sac and the virgin uterus. Two of these bands were evident in metrial gland up to day 16 of pregnancy, and a third became present by day 17. Unlike the other two bands, this new band did not seem to be associated with the large granules of the granulated metrial gland cells. None of these bands were detected in trophoblast. The metrial gland isoenzymes reacted as well at acid pH as at neutral pH. The yolk sac isoenzymes reacted either as well or slightly better at acid pH, and one extra band was demonstrable under acid conditions.
D000115:Acetylesterase / Q000032:analysis*; D000818:Animals; D005260:Female; D007527:Isoenzymes / Q000032:analysis*; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D051381:Rats; D014599:Uterus / Q000201:enzymology*
D007527:Isoenzymes; D000115:Acetylesterase