Interaction of contractile proteins with DNA.
The interaction of contractile proteins (myosin, actin, tropomyosin and troponin) with DNA was studied in vitro using a nitrocellulose filter binding technique. The data indicate a high affinity of myosin and troponin for DNA, a lesser interaction between DNA and tropomyosin and the absence of binding of actin to DNA. When binding to DNA was detected, the interaction was higher with single-stranded DNA than with RNA or double-stranded DNA, although in some conditions myosin binds equally as well to native as to denatured eukaryotic DNA. Myosin binds better to eukaryotic than to phage native DNA.
D000199:Actins; D000818:Animals; D002118:Calcium; D003285:Contractile Proteins; D004247:DNA; D007700:Kinetics; D009218:Myosins; D011485:Protein Binding; D013552:Swine; D014335:Tropomyosin; D014336:Troponin
D000199:Actins; D003285:Contractile Proteins; D014335:Tropomyosin; D014336:Troponin; D004247:DNA; D009218:Myosins; D002118:Calcium