[Normal values of the spontaneous cytochemical NBT test in adults].
The description is given of a working method adapted after Park, Fikrig and Smithwick, for testing the capacity of neutrophils to reduce tetrazolium nitroblue salts spontaneously. In contrast to the original technique, the present method uses : 10-20 units indigenous heparin/ml blood, leukocyte concentrate, other reaction times at 37 degrees C, contrast staining with a week Giemsa solution. Applied to young healthy subjects, the mean proportion of NBT-positive neutrophils was of 4.3 +/- 3.09 and the absolute number approximately less than 700/ml blood. The results obtained were consistent with the published data.
D000328:Adult; D006402:Hematologic Diseases / Q000175:diagnosis; D006801:Humans; D007958:Leukocyte Count; D009504:Neutrophils / Q000378:metabolism*; D009580:Nitroblue Tetrazolium; D010587:Phagocytosis; D012016:Reference Values; D013778:Tetrazolium Salts
D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium
Valori normale ale adultului la testul NBT citochimic spontan.