[Thiamine- and thiamine diphosphate-binding capacity of the hyaloplasmic fraction of rat liver].
Hyaloplasmic fraction of rat liver tissue did not bind 14C-thiamin and 14C-thiamin diphosphate in vitro as shown by means of gel filtration and equilibrium dialysis. All the thiamin and 2/3 of thiamin diphosphate were in vivo in free state or in a state unbound with proteins. Hyaloplasm lost readily the endogenous thiamin diphosphate during dialysis. Endogenous thiamin diphosphate (about 1/3 of the initial content), resistent to dialysis and gel filtration, was apparently included in transketolase in hyaloplasmic fraction of rat liver tissue.
D000818:Animals; D003593:Cytoplasm / Q000201:enzymology / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051381:Rats; D013831:Thiamine / Q000378:metabolism*; D013835:Thiamine Pyrophosphate / Q000378:metabolism*; D014174:Transketolase / Q000378:metabolism
D014174:Transketolase; D013835:Thiamine Pyrophosphate; D013831:Thiamine
Izuchenie tiamin- i tiamindifosfatsviazyvaiushcheĭ sposobnosti gialoplazmaticheskoĭ fraktsii pecheni krys.