title: A potential artifact in use of a new technique for rapid brain removal.
abstract: New techniques are are continuously being developed to obtain animal brain tissue that is as close as possible to its in vivo status. The brain-blowing technique provides a swift freezing of brain tissue (within 1 s) in which postmortem changes have been said to be minimal. Higher concentrations of glucose were present in brain samples removed by this method than have previously been found when the brain was frozen in situ, but at the same time the plasma levels of glucose were higher. It is suggested that, although the procedure did not appear to involve any stress, the hyperglycemia was stress related. Modifications of the procedure are described, by which these changes in blood glucose have been minimized.
mesh_terms: D000818:Animals; D001786:Blood Glucose; D001921:Brain / Q000378:metabolism*; D005615:Freezing; D005947:Glucose / Q000378:metabolism*; D008297:Male; D009482:Neurophysiology / Q000379:methods*; D051381:Rats; D013312:Stress, Physiological / Q000097:blood
chemical_list: D001786:Blood Glucose; D005947:Glucose