Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells.
Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.
D000546:Amanitins / Q000494:pharmacology; D002453:Cell Cycle; D002467:Cell Nucleus / Q000201:enzymology*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006367:HeLa Cells / Q000201:enzymology*; D006657:Histones / Q000096:biosynthesis*; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D012319:RNA Polymerase II / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects
D000546:Amanitins; D006657:Histones; D012333:RNA, Messenger; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases