The micro-ELISA technique in the serodiagnosis of visceral leishmaniasis.
The micro-scale Enzyme-linked Immunosorbent Assay (micro-ELISA) technique, with alkaline phosphatase as a marker enzyme, has been used for the serodiagnosis of human and canine visceral leishmaniasis, from foci in southern France. This technique which uses a soluble antigen is highly sensitive, with a degree of specificity slightly higher than that of the IFA. It is suggested that the micro-ELISA procedure can be added to the battery of serological techniques and would be particularly useful for large scale epidemiological studies of leishmaniasis.
D000469:Alkaline Phosphatase; D000818:Animals; D000906:Antibodies / Q000032:analysis; D004283:Dog Diseases / Q000175:diagnosis; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000276:immunology / Q000662:veterinary
D000906:Antibodies; D000469:Alkaline Phosphatase