title: Isolation and purification of moniliformin.
abstract: A bulk purification procedure is described for moniliformin, a mycotoxin produced by Fusarium moniliforme. The method involves methanol extraction of suitably molded maize, aqueous extraction of the methanol-free residue, ion exchange chromatography with a NaCl concentration gradient, desalination, and crystallization. A pKa value of 1.70 as well as molar absorptivities of 19100 (lambda (H2O) max 229 nm), 5600 (lambda (H2O) max 260 nm), and 4700 (lambda (MeOH) max 260 nm) L/mole/cm are reported for moniliformin.
mesh_terms: D003503:Cyclobutanes / Q000302:isolation & purification*; D009183:Mycotoxins / Q000302:isolation & purification*; D003313:Zea mays / Q000032:analysis
chemical_list: D003503:Cyclobutanes; D009183:Mycotoxins; C008539:moniliformin