Genetic effects of crocidolite asbestos in Chinese hamster lung cells.
Chinese hamster lung cells cultured in the presence of crocidolite asbestos displayed inhibition of cell growth. Cell death was directly associated with the phagocytosis of larger fibres as observed with the aid of trypan blue. Water soluble components of crocidolite did not appear to inhibit cell growth. Chromosomal aberrations were induced by the asbestos. The aberrations were confined mainly to structural aberrations--breaks and fragments. Electron-microscopic preparation indicated that asbestos was readily contained in phagosomes. Phagocytosed asbestos appeared to be a weak mutagen in its ability to induce gene mutation at the hypoxanthine-guanine phosphoribosyl transferase locus.
D001194:Asbestos / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects; D005796:Genes; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D009153:Mutagens; D010586:Phagocytes / Q000648:ultrastructure; D010587:Phagocytosis
D009153:Mutagens; D001194:Asbestos; D007041:Hypoxanthine Phosphoribosyltransferase