[Determination of ribonuclease reductase activity by thin layer chromatography on ECTEOLA-cellulose].
A rapid and sensitive method of measuring activity of ribonucleotide reductase has been developed. The method involves separation of ribonucleotides and desoxyribonucleotides in the ECTEOLA-cellulose thin layer, elution of desoxyribonucleotides and spectrophotometric assay of eluate extinction. The method utilizes an enzyme extracted from Propionibacterium shermanii.
D002855:Chromatography, Thin Layer / Q000379:methods; D012264:Ribonucleotide Reductases / Q000032:analysis*
D012264:Ribonucleotide Reductases
Opredelenie aktivnosti ribonukleotidreduktazy metodom tonkosloĭnoĭ khromatografii na EKTEOLA-tselliuloze.