Acetylcholinesterase differentiation during myogenesis in early chick embryonic cells caused by an inducer RNA.
In the stage 4 chick blastoderm, an area located 0.6 mm posterior to Hensen's node, the post-nodal piece (PNP), consists of an undifferentiated population of cells, since the explants when cultivated in vitro in a variety of media do not develop into any histologically identifiable structures. However, addition of a specific low molecular weight RNA isolated from the 16-day-old chick embryonic heart promotes the appearance of a distinct mode of morphological and biochemical changes that is similar to that of embryonic cardiogenic process. The RNA-induced changes in the PNP also include a marked increase in acetylcholinesterase activity. The increase in enzymatic activity can be measured biochemically, as well as visualized histochemically.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001756:Blastoderm / Q000201:enzymology; D002454:Cell Differentiation / Q000187:drug effects; D002642:Chick Embryo; D006321:Heart / Q000196:embryology; D006651:Histocytochemistry; D009132:Muscles / Q000196:embryology* / Q000201:enzymology; D012313:RNA / Q000302:isolation & purification / Q000494:pharmacology*
D012313:RNA; D000110:Acetylcholinesterase