Fluorogenic substrates for sensitive and differential estimation of urokinase and tissue plasminogen activator.
Two fluorogenic peptide amides have been synthesized, i.e. BOC-L-valyl-glycyl-L-arginine 2-naphthylamide (I) and L-valyl-glycyl-L-arginine 2-naphthylamide (II). The kinetic parameters of plasmin, urokinase and human uterine tissue plasminogen activator on substrates I and II have been determined. Quite unexpectedly, the tissue activator appeared to require for its activity a blocked amino terminus on the substrate. This was further corroborated with other synthetic substrates. Plasmin and urokinase did not show this requirement.
D000577:Amides / Q000378:metabolism; D010450:Endopeptidases; D005453:Fluorescence; D006801:Humans; D007700:Kinetics; D010455:Peptides / Q000378:metabolism; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator
D000577:Amides; D010455:Peptides; D010450:Endopeptidases; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator