Enzymatic synthesis of trisialoganglioside by particulate fractions of rat liver.
Rat liver contains a particulate (membrane-bound) glycosyl transferase, concentrated in Golgi apparatus fractions, which catalyzes the synthesis of a trisialoganglioside from the ganglioside precursor disialohematoside (GD3). Sialic acid was not incorporated into exogenous GD1a from CMP-N-acetylneuraminic acid suggesting that GD1a is an endproduct of the monosialoganglioside pathway. Thus, the disialoganglioside pathway may be a primary source of trisialoganglioside and higher ganglioside homologs in adult rat liver.
D000818:Animals; D005732:Gangliosides / Q000096:biosynthesis*; D006056:Golgi Apparatus / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism; D012799:Sialyltransferases / Q000378:metabolism*; D014166:Transferases / Q000378:metabolism*
D005732:Gangliosides; D012794:Sialic Acids; C016806:trisialoganglioside; D014166:Transferases; D012799:Sialyltransferases