Testosterone metabolites in dog bile.
Testosterone-1,2-3H was injected intravenously into a male dog with a bile fistula and bile and urine collected. The radioactivity was excreted preponderantly in bile (52% of the injected dose) in 6 hours; only 12% appeared in the urine. Methods to study the biliary metabolites of testosterone in this and other animals were developed. Satisfactory conjugate patterns were obtained by fractionation on DEAE-Sephadex A-25 columns using two different elution systems. In addition to an unchanged fraction, six different monoglucuronide fractions were separated. No other conjugates were isolated. Lipidex 5000 column chromatography, TLC and paper chromatography were used for the isolation and purification of aglycone metabolites, which were further identified by co-crystallization methods. The biliary metabolites of testosterone were epiandrosterone (3beta-hydroxy-5alpha-androstan-17-one), etiocholanlone (3alpha-hydroxy-5beta-androstan-17-one), 5alpha-androstan-3beta, 17beta-diol, 5beta-androstan-3alpha, 17beta-diol and 5beta-androstan-3beta,17beta-diol.
D000728:Androgens / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D001646:Bile / Q000378:metabolism*; D002852:Chromatography, Ion Exchange / Q000379:methods; D004285:Dogs / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism; D008297:Male; D013739:Testosterone / Q000378:metabolism* / Q000652:urine
D000728:Androgens; D013739:Testosterone; D005966:Glucuronidase