The use of polyethylene glycol in radioimmunoassay of staphylococcal enterotoxins.
The double-antibody radioimmunoassay of enterotoxins A, B, and C was modified by the addition of aqueous polyethylene glycol which precipitated double-antibody bound staphylococcal enterotoxins with little or no precipitation of free enterotoxin. The precipitate formed appeared as a thin coating at the bottom of the test tube and was not removed by aspiration. The procedure obviates the need for normal rabbit serum and for an additional wash of the precipitate.
D004768:Enterotoxins / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008461:Meat Products; D011092:Polyethylene Glycols; D011863:Radioimmunoassay / Q000379:methods*; D013210:Staphylococcus / Q000032:analysis*
D004768:Enterotoxins; D011092:Polyethylene Glycols