Assignment of the gene for lactic dehydrogenase A to mouse chromosome 7 using mouse-human hybrids.
We have studied somatic cell hybrids between either mouse peritoneal macrophages or spleen cells and HT-1080-6TG human fibrosarcoma cells for the expression of mouse lactic dehydrogenase A (LDH-A). The hybrids were also studied for the expression of mouse glucose phosphate isomerase (GPI-1), the gene for which has been assigned to chromosome 7. Concordant segregation of the expression of mouse GPI-1 and LDH-1 was observed in 61 independent hybrid clones. These results indicate that the gene coding for LDH-A is located on mouse chromosome 7.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002874:Chromosome Mapping; D002999:Clone Cells; D005354:Fibrosarcoma / Q000201:enzymology; D005796:Genes; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology*; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008264:Macrophages / Q000201:enzymology; D051379:Mice; D013154:Spleen / Q000166:cytology
D007770:L-Lactate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase