Binding of polynucleotides to fibroblasts. Effects of complex formation with vinyl analogs of nucleic acids.
The binding of polynucleotides or of their vinyl analogs to human fibroblasts is changed when complexes are formed between these compounds. The following polymers have been studied: poly(1-vinylcytosine), poly(1-vinyluracil, poly(9-vinyladenine), polyuridylate, polyadenylate and polyinosinate. Only that complex formation (between poly(1-vinylcytosine) and polyinosinate) which is accompanied by aggregation leads to a considerable increase (30 fold) in binding to cells; all the other complex formations have only moderate effects (0.2-3 fold). Furthermore, a comparison of unordered complexes containing polyinosinate whows that enhanced binding to cells is paralleled by an increased cellular resistance to viral infection.
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D011063:Poly A-U / Q000031:analogs & derivatives / Q000378:metabolism; D011070:Poly I-C / Q000031:analogs & derivatives / Q000378:metabolism; D011119:Polynucleotides / Q000378:metabolism*; D011145:Polyvinyls / Q000378:metabolism*
D011119:Polynucleotides; D011145:Polyvinyls; D011063:Poly A-U; D011070:Poly I-C