title: Subunit association and heterogeneity of Limulus polyphemus hemocyanin.
abstract: The molecular weights of the 6S, 24S, 36S, and 60S components of Limulus polyphemus hemocyanin were determined by high speed sedimentation equilibrium to be 69 400, 856 000, 1 690 000, and 3 160 000. The behavior of this hemocyanin appears to be similar to that of other arthropod hemocyanins where the first aggregation step is the formation of a hexamer of the 6S monomer. Here the larger aggregated states (24S, 36S, and 60S) are successive dimers of an unobserved hexamer (16S). The 24S-36S-60S association was found to be heterogeneous, suggesting that 24S components of different composition may be present.
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D006433:Hemocyanins; D006737:Horseshoe Crabs / Q000378:metabolism*; D046911:Macromolecular Substances; D008433:Mathematics; D008970:Molecular Weight; D014461:Ultracentrifugation
chemical_list: D046911:Macromolecular Substances; D006433:Hemocyanins