title: The calcium current of Helix neuron.
abstract: Calcium current, Ica, was studied in isolated nerve cell bodies of Helix aspersa after suppression of Na+ and K+ currents. The suction pipette method described in the preceding paper was used. Ica rises to a peak value and then subsides exponentially and has a null potential of 150 mV or more and a relationship with [Ca2+]o that is hyperbolic over a small range of [Ca2+]o's. When [Ca2+]i is increased, Ica is reduced disproportionately, but the effect is not hyperbolic. Ica is blocked by extracellular Ni2+, La3+, Cd2+, and Co2+ and is greater when Ba2+ and Sr2+ carry the current. Saturation and blockage are described by a Langmuir adsorption relationship similar to that found in Balanus. Thus, the calcium conductance probably contains a site which binds the ions referred to. The site also appears to be voltage-dependent. Activation and inactivation of Ica are described by first order kinetics, and there is evidence that the processes are coupled. For example, inactivation is delayed slightly in its onset and tau inactivation depends upon the method of study. However, the currents are described equally well by either a noncoupled Hodgkin-Huxley mh scheme or a coupled reaction. Facilitation of Ica by prepulses was not observed. For times up to 50 ms, currents even at small depolarizations were accounted for by suitable adjustment of the activation and inactivation rate constants.
mesh_terms: D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D003035:Cobalt / Q000494:pharmacology; D006372:Helix, Snails; D066298:In Vitro Techniques; D007811:Lanthanum / Q000494:pharmacology; D008839:Microelectrodes; D009431:Neural Conduction; D009474:Neurons / Q000378:metabolism*; D009532:Nickel / Q000494:pharmacology; D010477:Perfusion; D011188:Potassium / Q000502:physiology; D012964:Sodium / Q000502:physiology
chemical_list: D002104:Cadmium; D003035:Cobalt; D007811:Lanthanum; D009532:Nickel; D012964:Sodium; D011188:Potassium; D002118:Calcium