Elevated glucose levels influence in vitro hatching, attachment, trophoblast outgrowth and differentiation of the mouse blastocyst.
A glucose concentration of 3.5 mg/ml is optimal for in vitro embryo attachment and trophoblastic cell outgrowth. Raising the concentration above 3.5 mg/ml does not improve embryo culture and can at certain concentrations be detrimental to embryo development.
D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media; D004622:Embryo, Mammalian / Q000187:drug effects / Q000502:physiology; D005947:Glucose / Q000494:pharmacology* / Q000502:physiology; D051379:Mice; D009924:Organ Culture Techniques; D014327:Trophoblasts / Q000502:physiology
D003470:Culture Media; D005947:Glucose