Increased purine nucleotide binding, altered polypeptide composition, and thermogenesis in brown adipose tissue mitochondria of cold-acclimated rats.
Rapid increases in atractyloside-insensitive binding of purine nucleotides (ADP or GDP) and in a polypeptide of 32 000 occur in brown adipose tissue mitochondria of the rat during acclimation to cold. The increased binding is apparent within 1 h and reaches a maximum after 3--7 days of exposure to 4 degrees C. The increase in the 32 000 peptide occurs more slowly and reaches a maximum after 2--3 weeks. There is a simultaneous decrease in a polypeptide of 96 000, apparent after 1 day and reaching a maximum after 1--2 weeks. Results are interpreted in terms of the appearance of an increased amount of the purine nucleotide-sensitive proton conductance pathway in association with the development of an enhanced thermogenic capacity of brown adipose tissue mitochondria during acclimation of the rat to cold.
D000064:Acclimatization; D000244:Adenosine Diphosphate / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D003080:Cold Temperature; D004734:Energy Metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D011685:Purine Nucleotides / Q000378:metabolism*; D051381:Rats
D010455:Peptides; D011685:Purine Nucleotides; D006153:Guanosine Diphosphate; D000244:Adenosine Diphosphate