[Radioimmunoassay of 125I-sagamicin (author's transl)].
A radioimmunoassay for an aminoglycoside antibiotic, sagamicin, was developed using antisera from rabbits injected with a sagamicin-bovine serum albumin conjugate. Sagamicin was iodinated by a modified Bolton & Hunter method. The standard curve was linear on a logit-log plot yielding an sensitivity of 0.5ng/tube. A correlation coefficient of 0.95 was obtained between the radioimmunoassay and a microbioassay for sagamicin in human sera. Cross-reaction occurred with gentamicin components and some of sagamicin subunits, but there was no cross-reactivity to neomycin, kanamycin and amikacin. Antibody was purified by affinity chromatography on sagamicin bound agarose.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000917:Antibody Formation; D002846:Chromatography, Affinity; D003429:Cross Reactions; D005839:Gentamicins / Q000032:analysis* / Q000276:immunology; D007457:Iodine Radioisotopes; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*
D000906:Antibodies; D005839:Gentamicins; D007457:Iodine Radioisotopes; C037545:micronomicin