[Heterogeneity of leghemoglobin from yellow lupine nodules].
A possibility is demonstrated to separate summary lupine leghemoglobins (which are salted out within 55--90% of ammonium sulphate saturation) into Lb I and Lb II components by means of ionic exchange chromatography on DEAE-cellulose. Lb I is eluted at lower ionic strength buffer than LbII, and differs from the latter in the form and the size of crystals. Both components have the same electrophoretic mobility and contain N-terminal glycine. LbII and LbI precipitate under gradual salting out within 55--75% and 78--90% of saturation respectively.
D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D006420:Hemeproteins / Q000302:isolation & purification*; D007874:Leghemoglobin / Q000302:isolation & purification*; D010944:Plants
D006420:Hemeproteins; D007874:Leghemoglobin
O geterogennosti leggemoglobinov iz kluben'kov zheltogo liupina.