The stability of A1,B29-adipoylinsulin to chemical and enzymatic degradation.
The degradation of A1,B29-adipolyinsulin by partial hydrolysis, by disulphide-bond exchange, by chymotrypsin and by a cytosolic enzyme fraction of rat liver was measured and compared with that of A1,B29-diacetylinsulin and insulin. The cross-linked insulin is, in general, more resistant than insulin itself or the diacetylated derivative. However, in the case of enzymatic degradation, A1,B29-diacetylinsulin resembles more the cross-linked derivative than insulin. This provides evidence that the properties of A1,B29-adipoylinsulin could not in all circumstances be attributed to its 'proinsulin-like' structure.
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin; D003600:Cytosol / Q000201:enzymology; D004355:Drug Stability; D006868:Hydrolysis; D007328:Insulin / Q000031:analogs & derivatives / Q000032:analysis*; D051381:Rats
D007328:Insulin; D002918:Chymotrypsin