title: [Cytospectrophotometric study of GABA-transaminase in the rat cerebellar cortex].
abstract: Modification of the histochemical method for detection of GABA-transaminase activity is suggested. Optimal concentrations of the substrates and cofactors are chosen on the basis of kinetic study of the enzymatic reaction in the cryostat sections of the rat cerebellar cortex by the quantitative microspectrophotometric method. The method is intended for the quantitative histochemical analysis of GABA-transaminase activity in the brain sections.
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000032:analysis*; D000818:Animals; D002525:Cerebellar Cortex / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008297:Male; D051381:Rats; D013053:Spectrophotometry / Q000379:methods; D013385:Succinate Dehydrogenase / Q000032:analysis; D000637:Transaminases / Q000032:analysis*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D005680:gamma-Aminobutyric Acid; D013385:Succinate Dehydrogenase; D000637:Transaminases; D000612:4-Aminobutyrate Transaminase
vernacular_title: Tsitospektrofotometricheskoe issledovanie GAMK-transaminazy v kore mozzhechka krys.