Stimulation of calcium transport in inside-out vesicles of human erythrocyte membranes by a soluble cytoplasmic activator.
Transport of Ca2+ by inside-out vesicles requires both Mg2+ and ATP and can be linear over 16 min at 37 degrees C. This basal rate of transport may be doubled however by an activator found in membrane-free erythrocyte hemolysate. This activatior is probably the same protein (s) which has been shown to activate (Ca2+ + Mg2+)-ATPase in erythrocyte membrane fragments (Bond, G.H. and Clough, D.E. (1973) Biochim. Biophys. Acta 323, 592--599).
D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D012995:Solubility
D011506:Proteins; D002118:Calcium