title: A high performance liquid chromatographic system for the analysis of tetracycline drug standards, analogs, degradation products and other impurities.
abstract: This paper describes the high performance liquid chromatographic (HPLC) analysis of eight parent tetracycline standards: tetracycline, chlortetracycline, rolitetracycline, oxitetracycline, minocycline, doxycycline, democlocycline, methacycline; and three tetracycline epimers: epitetracycline, epianhydrotetracycline, and anhydrotetracycline. The HPLC system employs an octadecylsilane reverse phase column and an isopropanol-diethanolamine-phosphate-ammonium EDTA-water mobile phase. This system produced at least partial resolution of all eight parent compounds and many of their degradation products.
mesh_terms: D002751:Chlortetracycline / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004318:Doxycycline / Q000032:analysis; D008690:Methacycline / Q000032:analysis; D008911:Minocycline / Q000032:analysis; D010118:Oxytetracycline / Q000032:analysis; D012016:Reference Values; D012382:Rolitetracycline / Q000032:analysis; D013237:Stereoisomerism; D013752:Tetracycline / Q000032:analysis; D013754:Tetracyclines / Q000032:analysis*
chemical_list: D013754:Tetracyclines; D013752:Tetracycline; D008911:Minocycline; D012382:Rolitetracycline; D008690:Methacycline; D004318:Doxycycline; D002751:Chlortetracycline; D010118:Oxytetracycline