[A radioimmunoassay for plasma 11-desoxycortisol and its use in the rapid metopirone test].
A radioimmunoassay for the measurement of 11-deoxycortisol in plasma is described. Antiserum against 11-deoxycortisol was produced by immunizing rabbits with the 21-hemisuccinate of 11-deoxycortisol coupled to bovine serum albumin. The method does not require chromatography but instead makes use of a simple extraction procedure which, in combination with the antibody characteristics, is relatively specific for the 11-deoxycortisol determination. The smallest amount measurable is 5 pg. The intra-assay coefficient of variation was 6.3% before metopirone and 7.2% after metopirone. The inter-assay coefficient of variation was 12.5% before metopirone and 10.3% after metopirone. Pituitary-adrenal reserve was evaluated in control and hypopituitary subjects by a simple midnight metopirone test.
D015065:17-Hydroxycorticosteroids / Q000097:blood*; D003350:Cortodoxone / Q000097:blood*; D006801:Humans; D007018:Hypopituitarism / Q000175:diagnosis*; D008797:Metyrapone; D011863:Radioimmunoassay
D015065:17-Hydroxycorticosteroids; D003350:Cortodoxone; D008797:Metyrapone
Ein Radioimmunoassay für 11-Desoxykortisol im Plasma und die Anwendung beim Metopironkurztest.