High-pressure liquid chromatography in polynucleotide synthesis.
Reverse phase high-pressure liquid chromatography (HPLC) using columns containing microparticulate materials with bonded octadecyl groups has been developed as a rapid and efficient method for the separation of nucleosides, nucleotides, and, in particular, of protected oligonucleotides which are standard intermediates in the stepwise synthesis of deoxyribopolynucleotides. Reported are extensive studies of the influence of the different purine and pyrimidine bases, of protecting groups, of the phosphate groups, and of the chain lengths of oligonucleotides on their retention on such columns. Further, the application of HPLC in the stepwise synthesis of an oligonucleotide, d(G-G-A-A-G-C-T-T-A-A-C), has been described. The methods, which are herein described, lend themselves to separations on a preparative scale and effect a marked reduction (up to 50%) in the time required for the synthesis of oligonucleotides.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000138:chemical synthesis*; D003853:Deoxyribonucleosides / Q000138:chemical synthesis; D003854:Deoxyribonucleotides / Q000138:chemical synthesis; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification
D003853:Deoxyribonucleosides; D003854:Deoxyribonucleotides; D009838:Oligodeoxyribonucleotides; D004247:DNA