A comparison of t-butyldimethylsilyl and trimethylsilyl ether derivatives for the characterization of urinary metabolites of prostaglandin F2alpha by gas chromatography mass spectrometry.
t-Butyldimethylsilyl and trimethylsilyl ether derivatives of a series of methylated prostaglandin F2alpha metabolites have been compared with respect to their gas chromatographic and mass spectrometric properties. The t-butyldimethylsilyl derivatives had considerably higher retention indices (approximately 2.2 C units per silyl group) than their trimethylsilyl ether counterparts when analysed on the (non-polar) OV-1 stationary phase. Electron impact induced fragmentation patterns were strongly dependent upon the type of silyl ether employed and on the nature of the prostaglandin omega sidechain; the mass spectra of pairs of t-butyldimethylsilyl and trimethylsilyl ether derivatives were found to differ appreciably in several respects and to afford complementatary structural information.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D004987:Ethers / Q000652:urine; D007783:Lactones / Q000652:urine; D008297:Male; D013058:Mass Spectrometry; D011460:Prostaglandins F / Q000652:urine*; D051381:Rats; D012825:Silicon; D014297:Trimethylsilyl Compounds
D004987:Ethers; D007783:Lactones; D011460:Prostaglandins F; D014297:Trimethylsilyl Compounds; D012825:Silicon