Platelet activation: a new biological activity of guinea-pig C3a anaphylatoxin.
3H-serotonin-release from labelled gp-platelets is established as a sensitive method for testing a new biological activity of gp-C3a anaphylatoxin in an autologous situation. Time-, dose- and temperature-dependent release reactions as well as specific inhibition by carboxypeptidase B and anti-C3a antibodies show that C3a is a potent and specific inducer of platelet activation. Inactive C3a does not induce 3H-serotonin-release but specifically inhibits the action of C3a on platelets.
D000706:Anaphylatoxins / Q000276:immunology*; D000818:Animals; D001792:Blood Platelets / Q000276:immunology*; D002860:Chromium Radioisotopes; D003176:Complement C3; D003169:Complement Inactivator Proteins / Q000494:pharmacology; D006168:Guinea Pigs; D010455:Peptides / Q000276:immunology*; D012701:Serotonin / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014316:Tritium
D000706:Anaphylatoxins; D002860:Chromium Radioisotopes; D003176:Complement C3; D003169:Complement Inactivator Proteins; D010455:Peptides; D014316:Tritium; D012701:Serotonin