Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxic complex.
Type A botulinum neurotoxin was purified from toxic crystals by adsorption to p-aminophenyl-beta-D-thiogalactopyranoside coupled to CH-Sepharose 4B. At pH 6.3, the toxic complex was held by the binding between the ligand and the hemagglutinin of the complex; the toxin is eluted selectively by dissociating the complex with buffer-saline of pH 7.9. The single-step affinity chromatography recovered 50 to 60% of applied toxicity as preparations of greater than 99% purity.
D000818:Animals; D001905:Botulinum Toxins / Q000302:isolation & purification* / Q000633:toxicity; D002846:Chromatography, Affinity; D003461:Crystallography; D006388:Hemagglutinins / Q000302:isolation & purification; D051379:Mice; D009498:Neurotoxins / Q000302:isolation & purification* / Q000633:toxicity
D006388:Hemagglutinins; D009498:Neurotoxins; D001905:Botulinum Toxins