Lysine tRNAs from cytoplasm, chloroplasts and mitochondria of the lupin seedling cotyledons.
1. The lupin cotyledons contain two cytoplasm-specific lysine tRNAs, one chloroplast-specific, one mitochondria-specific species, and three species common to all the cell compartments investigated; the lysine tRNAs were separated by reversed-phase chromatography (RPC-5). 2. The two cytoplasmic lysine tRNAs are predominant in the tRNALys isolated from the whole tissue. Their level and relative ratios change during germination (Augustyniak & Pawełkiewicz, 1978, Phytochemistry, 17, 15-18). 3. The organellar tRNA constitutes about 20% of the total tRNA in the cotyledons of 7-day-old lupin seedlings.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification; D002736:Chloroplasts / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D007887:Fabaceae / Q000032:analysis*; D008928:Mitochondria / Q000032:analysis; D010946:Plants, Medicinal; D012343:RNA, Transfer / Q000032:analysis*
D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases