Chymotryptic and tryptic peptides of fragment alpha 1-CB3 from bovine corneal collagen. Pinpointing the sites of hexose attachment.
Tryptic peptides of citraconylated fragment alpha1-CB3 and chymotryptic peptides of fragment alpha1-CB3 of bovine corneal collagen were prepared, isolated and characterized. Their amino acid compositions were consistent with the amino acid sequence of fragment alpha1-CB3 from calf skin collagen. Two glycoside sites were identified in bovine corneal fragment alpha1-CB3, one of them being the first located in the overlap region of collagen. The results are related to the uniformly narrow collagen fibres found in cornea and essential for its transparency.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002918:Chymotrypsin; D003094:Collagen / Q000032:analysis*; D003315:Cornea / Q000032:analysis*; D006027:Glycosides / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis*; D014357:Trypsin
D006027:Glycosides; D010446:Peptide Fragments; D003094:Collagen; D002918:Chymotrypsin; D014357:Trypsin