The identification and quantification of three new 6alpha-hydroxylated corticosteroids in human neonatal urine.
The 24 hours urines of six two days old fullterm newborn infants were investigated for polar corticosteroids. 6alpha-hydroxy-tetrahydrocortisone, 6alpha-hydroxy-20alpha-cortolone and 6alpha-hydroxy-20beta-cortolone were identified by gas chromatographic-mass spectrometric comparison of the urinary steroids to compounds synthesized previously. These 6alpha-hydroxylated corticosteroids as well as seven other polar corticosteroids were quantified by gas chromatography or mass fragmentography. It was shown that the newly identified steroids constituted a quantitatively important part of the neonatal urinary corticosteroids. The unconjugated- and glucuronic acid conjugated steroids were quantified separately. It was found that the extent of glucuronoconjugation decreased with increasing polarity of the steroid moiety.
D003348:Cortisone / Q000031:analogs & derivatives*; D005965:Glucuronates / Q000652:urine; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D006889:Hydroxycorticosteroids / Q000652:urine*; D007231:Infant, Newborn; D008297:Male; D013058:Mass Spectrometry; D011278:Pregnanes / Q000652:urine*; D013761:Tetrahydrocortisone / Q000031:analogs & derivatives* / Q000652:urine
D005965:Glucuronates; D006889:Hydroxycorticosteroids; D011278:Pregnanes; D013761:Tetrahydrocortisone; D003348:Cortisone; D006854:Hydrocortisone