Nature of inhibition of rat testicular alkaline phosphatase by isatin.
Isatin has been found to inhibit rat testicular alkaline phosphatase (EC 3.1.3.1). That the inhibition is non-competitive as well as non-allosteric is evident from a) the hyperbolic curve relating inhibition as a function of inhibitor concentration; b) the small change in enthalpy, free energy and entropy; c) the number of isatin molecules associating with 1 molecule of the enzyme (n=1.29); and, d) the decrease in the values of both Km and Vmax in the presence of isatin.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000818:Animals; D007211:Indoles / Q000494:pharmacology*; D007510:Isatin / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D051381:Rats; D013737:Testis / Q000201:enzymology*
D007211:Indoles; D007510:Isatin; D000469:Alkaline Phosphatase