Histochemical demonstration of type IV collagen in the renal glomerulus.
Disulfide-groups are demonstrated in the basement membranes of glomerular capillaries, proximal convoluted tubules, and collecting ducts by means of a thiosulfathion/Alcian Blue +0.8 Mol MgCl2-staining sequence. It is suggested that the reaction shows type IV collagen of basal lamina material, which is characterized by a relatively high cystine content (8 half-cystine residues/1000).
D000818:Animals; D001485:Basement Membrane / Q000032:analysis; D003094:Collagen / Q000032:analysis*; D004220:Disulfides / Q000032:analysis; D006168:Guinea Pigs; D006651:Histocytochemistry; D007678:Kidney Glomerulus / Q000032:analysis*
D004220:Disulfides; D003094:Collagen