Infrared stretching frequencies of CO in carbomonoxyhemoglobin from trout.
The infrared spectra of the carbomonoxy derivatives of the hemoglobin components I and IV from trout have been measured in the CO stretching frequency region using a high resolution infrared spectrometer. The CO stretching frequency of Hb I CO is very close to that of carbomonoxy human hemoglobin and is pH-independent. In contrast, the CO stretching frequency of Hb IV CO is higher and shows a small but significant pH dependence in the range 6.2-7.8. These results point to a decreased strength of the iron-CO bond in Hb IV CO at low pH, in agreement with the conclusions drawn from the reported difference spectra of Hb IV CO as a function of pH.
D000818:Animals; D002248:Carbon Monoxide; D002263:Carboxyhemoglobin; D006454:Hemoglobins; D006801:Humans; D013055:Spectrophotometry, Infrared; D014337:Trout
D006454:Hemoglobins; D002248:Carbon Monoxide; D002263:Carboxyhemoglobin