Comparison of alkaline phosphatase isoenzymes determined by an inhibition method and by electrophoresis.
A chemical inhibition procedure suitable for the routine determination of alkaline phosphatase (AP) isoenzymes in serum has been adapted for use with a fast kinetic analyzer, System Olli 3000. The results of this procedure are compared with the electrophoretic separation of alkaline phosphatase isoenzymes. The comparison of the results obtained indicates that the AP-urea/AP ratio can be used to differentiate between patients with bone and liver disease and that it is possible to estimate the relative bone and liver isoenzyme activities from this ratio quickly using two simple equations.
D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis* / Q000037:antagonists & inhibitors; D001842:Bone and Bones / Q000201:enzymology; D002648:Child; D002675:Child, Preschool; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006801:Humans; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis*; D008099:Liver / Q000201:enzymology; D008722:Methods; D011247:Pregnancy
D007527:Isoenzymes; D000469:Alkaline Phosphatase