title: Analysis of deoxynivalenol from cultures of Fusarium species.
abstract: Eight isolates of Fusarium roseum and three of Fusarium colmorum were found to produce deoxynivalenol in rice cultures. Deoxynivalenol was extracted with aqueous methanol (40%) and purified by partitioning with ethyl acetate and acetonitrile-petroleum ether (boiling point, 60--70 degrees C). The toxin was identified by gas chromatography/mass spectrometry and quantified by gas-liquid chromatography. High recoveries (80%) of deoxynivalenol were obtained from rice cultures, and as low as 0.250 microgram of the toxin per g was detected.
mesh_terms: D003470:Culture Media; D005670:Fusarium / Q000032:analysis / Q000378:metabolism*; D012275:Oryza; D012717:Sesquiterpenes / Q000096:biosynthesis*; D013045:Species Specificity; D014255:Trichothecenes / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D012717:Sesquiterpenes; D014255:Trichothecenes