Extraction of daunorubicin and doxorubicin and their hydroxyl metabolites: self-association in aqueous solution.
The extraction of daunorubicin and doxorubicin and their hydroxyl metabolites daunorubicinol and doxorubicinol was studied using chloroform-1-pentanol (9:1) as the organic phase. Because of differences in acid dissociation constants, the pH for optimum extraction varied from 8.0 to 8.6 for the different compounds. Self-association in the aqueous phase significantly influenced the distribution ratio. Constants for the formation of dimers and tetramers in aqueous solutions were about 10(4.5) and 10(12), respectively.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003630:Daunorubicin / Q000032:analysis / Q000302:isolation & purification*; D004317:Doxorubicin / Q000032:analysis / Q000302:isolation & purification*; D008027:Light; D008956:Models, Chemical; D012995:Solubility; D012996:Solutions; D013053:Spectrophotometry; D014867:Water
D012996:Solutions; D014867:Water; D004317:Doxorubicin; D003630:Daunorubicin