[Ribosomal RNA degradation in rat skeletal muscle in the process of autolysis].
Comparative histochemical study of RNA content in normal and autolyzed within 72 hrs muscle tissue showed that the nucleic acid well retained during autolysis but the tissue was simultaneously destructed. Polymerization of rRNA in autolyzed tissue proved to depend on temperature of dead animals storage. An increase of temperature from 0 degrees to 21 degrees did not affect the rRNA yield. But amount of latent breaks, which were found in rRNA by methods of formamide and temperature treatment, was increased depending on increase in temperature and on length of animal storage after death. The latent breaks appear to be accumulated in animal tissues during autolysis.
D000818:Animals; D001329:Autolysis / Q000378:metabolism*; D005559:Formamides / Q000494:pharmacology; D009132:Muscles / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D013696:Temperature; D013997:Time Factors
D005559:Formamides; D012335:RNA, Ribosomal
Izuchenie degradatsii ribosomnoĭ RNK v skeletnoĭ myshtse krys v prptsesse avtoliza.