[Structural characteristics and oxidative phosphorylation of the isolated mitochondria from the rabbit small intestine epithelium under normal conditions and under the effect of choleragen].
Mitochondrial fraction was isolated from the rabbit small intestine epithelium by differential centrifugation in isotonic sucrose medium. The fraction was insignificantly contaminated with fragments of other organellae and microvilli. Respiratory control coefficient of such mitochondria constituted 3-5, when malate-glutamate mixture was used as a substrate. Changes in the functional state of mitochondria were accompanied by stereotypical reconstruction of the "orthodox-condensed" configuration. Introduction of crude choleragen into the mitochondria incubation medium failed to induce any changes in the rate of oxygen consumption in states III and IV (after Chance).
D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology*; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D010085:Oxidative Phosphorylation; D011817:Rabbits
D002772:Cholera Toxin
Izuchenie strukturnykh osobennosteĭ i okislitel'nogo fosforilirovaniia izolirovannykh mitokhondriĭ epiteliia tonkoĭ kishki krolika v norme i pri vozdeĭstvii kholeragenom.