Stimulation of protein synthesis in isolated hepatocytes by somatomedin.
Insulin (1 mU/ml) stimulated the incorporation of 14C-leucine into trichloroacetic acid-precipitable material by isolated hepatocytes from normal and hypophysectomized adult rats and 12-day-old rabbits. Somatomedin (200 ng/ml) purified from human plasma had an insulinlike effect in hepatocytes from hypophysectomized rats and baby rabbits but not from normal rats. This study suggests that, as well as being a site of somatomedin synthesis, the liver may be a target organ for this hormone.
D000818:Animals; D005260:Female; D007016:Hypophysectomy; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014176:Protein Biosynthesis; D011817:Rabbits; D051381:Rats; D013002:Somatomedins / Q000494:pharmacology*; D013268:Stimulation, Chemical
D007328:Insulin; D013002:Somatomedins