Relation of cytolytic activity of an ether-deoxy lysophosphatidylcholine analog to available membrane surface. Comparison of normal and tumor cells from mice.
1-Hexadecylpropanediol-3-phosphorylcholine, an ether-deoxy analog of lysophosphatidylcholine, has been employed to study the sensitivity of various types of mouse cells with respect to changes in membrane permeability induced by lysophosphatidylcholine. Cells used included erythrocytes, thymocytes, spleen cells and macrophage, as well as 4 different tumors (2 lymphomas, 1 Ehrlich acites and 1 methylcholanthren-induced fibrosarcoma). The sensitivity to the lysophosphatide (on a per-cell basis) of the above cell types varied by a factor of 65. When lytic concentrations were related to available membrane surface, this variation was reduced to a factor of 2.5. No principal difference was observed between the sensitivity of normal versus tumor cell membranes with respect to lysophosphatidylcholine lysis. Membrane surface, available for lysophosphatidylcholine, has been estimated from binding equilibria of 14C-labelled deoxy-lysophosphatidylcholine to the cells under standardized conditions. This method is based on the finding that binding equilibria of lysophospholipids to cells are predominantly determined by the available membrane surface.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008244:Lysophosphatidylcholines / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D009374:Neoplasms, Experimental / Q000503:physiopathology* / Q000648:ultrastructure; D011188:Potassium / Q000378:metabolism; D013045:Species Specificity; D013499:Surface Properties; D013696:Temperature
D008244:Lysophosphatidylcholines; D011188:Potassium