In situ Fc receptor-bearing cells in two murine tumors. I. Isolation and identification.
The in situ inflammatory response was studied in 2 different tumors, the T1699 mammary adenocarcinoma in inbred DBA/2 mice and a sarcoma induced in inbred C57BL/6 mice by murine sarcoma virus. The presence of Fc receptors (FcR's) on infiltrating host cells was determined by rosette formation with antibody-coated sheep red blood cells (EA). Differences in FcR-binding activity among various cells were detected with the use of EA prepared with a range of antibody concentrations. Cells bearing FcR represented one-third or more of the inflammatory cell population in both tumors. The FcR-positive cells were heterogeneous in regard to both cell type and FcR activity. Monocytes and macrophages had greater FcR activity than did lymphocytes. Different populations of FcR-positive cells could be isolated by their EA-binding characteristics.
D000818:Animals; D001665:Binding Sites; D002469:Cell Separation; D005260:Female; D007111:Immunity, Cellular; D007141:Immunoglobulin Fc Fragments; D008325:Mammary Neoplasms, Experimental / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000276:immunology*; D012397:Rosette Formation; D012513:Sarcoma, Experimental / Q000209:etiology / Q000276:immunology; D014412:Tumor Virus Infections / Q000276:immunology
D007141:Immunoglobulin Fc Fragments