[Production of staphylococcal alpha-hemolysin in a nutrient medium free of ballast substances].
A highly-active staphylococcus alpha-hemolysin was obtained in a synthetic nutrient medium containing inorganic salts, vitamins, glucose and casamine acids, with fractional addition of glucose, histidine, and NaHCO3 solutions into the medium during cultivation. The intensity of alpha-toxin production was directly proportional to the initial concentration in the medium of casamine acids (within the range of 0.2--2.0%). The presence of lecithin in the medium (0.04%) accelerated the appearance of alpha-hemolysin, and then suppressed its production.
D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000378:metabolism
D003470:Culture Media; D006460:Hemolysin Proteins
Produktsiia stafilokokkovogo al'fa-gemolizina v pitatel'noĭ srede, svbodnoĭ ot ballastnykh veshchestv.