title: A micro liquid column chromatography procedure for twelve anticonvulsants and some of their metabolites.
abstract: Solvent extracts of 50 microliter volumes of serum were sufficient for the determination of twelve anticonvulsant drugs. The liquid chromatography procedure utilized a C-18 reversed-phase column and isocratic elution with 15% acetonitrile in water. No derivatization was required. Eluted anticonvulsants were detected by UV absorption at 195 nm and quantitated by drug--internal standard peak area ratios. The procedure provided linear working curves over the concentration range from 1 to 100 mg/l of drug in the serum. The procedure for serum provided recoveries of the drugs from 92 to 101%. Within-day precision was about 4% and day-to-day precision was about 6.5%. The procedures has been applied to urine samples to facilitate bioavailability studies. Data are also given for several metabolites. There is a discussion of many practical aspects of the procedure to improve the reliability of the results.
mesh_terms: D000927:Anticonvulsants / Q000097:blood* / Q000652:urine; D001682:Biological Availability; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D008832:Microchemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000927:Anticonvulsants