title: A chamber attached to the SEM for fracturing and coating frozen biological samples.
abstract: A chamber for introducing, fracturing and coating frozen biological samples has been developed as an attachment to the sepcimen chamber of a scanning electron microscope. Together with a eucentric-tilt cold-stage, this chamber constitutes a complete system for viewing fractured biological surfaces of the type normally only seen by replica techniques. An air-lock on the chamber accepts a transfer module to allow insertion of the frozen sample without frost build-up. Fracturing is carried out with a precisely adjustable cooled knife under a 10--100X binocular microscope. The sample can tilt and rotate while being coated with carbon or metals evaporated from rechargeable sources introduced through the air-lock. Cooling in the chamber is provided by a cylindrical copper tank filled with liquid nitrogen. The chamber has its own LN2 trapped high vacuum system. After preparation the sample can be placed directly into the SEM through an isolation valve. The cold-stage utilizes a Joule-Thomson refrigerator. The sample can be kept below 103 K at all times though there are provisions for heating it in the fracturing and cold-stage positions. A system of controls, sensors and interlocks simplifies the operation of the system.
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D005614:Freeze Fracturing / Q000295:instrumentation*; D007687:Kidney Tubules, Proximal / Q000648:ultrastructure; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010944:Plants / Q000648:ultrastructure