Two initiation sites for adenovirus 5.5S RNA.
Adenovirus specific 5.5S RNA is heterogeneous at its 5' terminus. Complete pancreatic RNase digests of the RNA reveal a 5' terminal oligonucleotide (pp)pApGpCp in addition to the major 5' terminal (pp)pGpGpGpCp (1). Both 5' termini are detected early as well as late after adenovirus infection. In isolated nuclei, alpha-amanitin inhibits all 5.5S RNA transcription at a concentration of 200 microgram/ml, indicating that both initiation sites are recognized by RNA polymerase III.
D000227:Adenine Nucleotides; D000260:Adenoviruses, Human / Q000378:metabolism*; D000546:Amanitins / Q000494:pharmacology; D001483:Base Sequence; D006158:Guanosine Pentaphosphate; D006367:HeLa Cells; D008970:Molecular Weight; D012320:RNA Polymerase III / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D013997:Time Factors
D000227:Adenine Nucleotides; D000546:Amanitins; D012367:RNA, Viral; D006158:Guanosine Pentaphosphate; D012320:RNA Polymerase III