[Structure of nuclear pre-mRNA. IX. Hybridization of double-stranded portions of pre-mRNA with excess mRNA].
The hybridization of double-stranded regions of pre-mRNA from mouse Ehrlich ascites carcinoma cells, rabbit bone marrow cells and primary culture of rabbit kidney cells with an excess of total poly(A)+-mRNA of mouse or rabbit globin mRNA respectively was studied. The hybrids were detected as RNAase-stable acid precipitable material or by adsorbtion of the hybrid complexes of poly(U)-sepharose. The sizes of the hybrid complementary sequences and their thermal stability were estimated.
D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000032:analysis; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins; D007668:Kidney / Q000032:analysis; D051379:Mice; D009693:Nucleic Acid Hybridization; D011061:Poly A; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011817:Rabbits
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011061:Poly A; D005914:Globins
Struktura iadernoĭ pre-mRNK. IX. Gibridizatsiia dvuspiral'nykh uchastkov pre-mRNK s izbytkom mRNK.