Neuronal and non-neuronal components in the overflow of labelled adenyl compounds from guinea-pig taenia coli.
Guinea-pig taenia coli preprations were pre-incubated in 3H-adenosine and the overflow of 3H-compounds to a superfusing medium was measured during transmural field stimulation in the presence and absence of tetrodotoxin. The fractional overflow of 3H-compounds on stimulation of taenia coli pre-incubated in 3H-adenosine at 10(-7) M was greater than after incubation at 10(-5) M. About half of the releasable store was blocked by tetrodotoxin. It is proposed that at lower concentrations, there is selective loading of a releasable store, while at higher concentrations thare is a greater loading of non-releasable stores, and that neuronal stores account for about 33% of the 3H-compounds released by transmural stimulation.
D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D003106:Colon / Q000294:innervation / Q000378:metabolism*; D004558:Electric Stimulation; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology
D013779:Tetrodotoxin; D000241:Adenosine; D009638:Norepinephrine