title: Multicellular tumor spheroid formation by breast cancer cells isolated from different sites.
abstract: Fourteen breast cancer lines (8 human, 5 rat, and 1 mouse) have been studied in terms of their ability to form multicellular tumor spheroids (MTS) with the agar-base method. Only 8 of the lines formed MTS in contrast to a 100% efficiency in a series of 11 varied tumors reported in the initial studies with this method. We have compared the lines that do and do not form MTS in terms of a variety of characteristics (e.g., estrogen receptors, time in serial passage, growth in nude mice, etc.), and only one characteristic, the source of the original tumor cells, was predictive of MTS-forming ability. All 8 of the breast cancer lines (and the original 11 lines) that formed MTS had been obtained from solid growths (primaries or metastases), while the 6 breast cancer lines that did not form MTS were all derived from pleural effusions. Similarly, artificial selection for an ascites variant of the MTS-forming rat 13762 adenocarcinoma line produced the 13762-A line, which could no longer form MTS. These results suggest that breast cancer cells derived from pleural effusions are genetically different from the bulk of the tumor cells in solid breast cancer samples, that they are unable to grow in true solid form, and that these differences persist in spite of prolonged propagation in tissue culture.
mesh_terms: D000222:Adaptation, Physiological; D000362:Agar; D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology* / Q000503:physiopathology; D002449:Cell Aggregation; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000473:pathology* / Q000503:physiopathology; D051379:Mice; D009362:Neoplasm Metastasis / Q000473:pathology; D009374:Neoplasms, Experimental / Q000473:pathology; D010996:Pleural Effusion / Q000166:cytology; D051381:Rats
chemical_list: D000362:Agar