[Incorporation of glycine(14C) into liver proteins during low energy laser pulse irradiation].
The authors examined the action of irradiation with ruby lazer at lambda=6,948 A and level of energy 0.13 j/sm2. Liver of rats was irradiated through the surrounding tissues in five successive impulses. The skin in the region of action was stained with methylene blue. Changes in the incorporation of the label in the free amino-acids, in total cholesterol of liver and ribosomal protein as well as the proteins in the supernatant over ribosomes were examined. Glycine (14C) was identified by chromatographic was and autoradiographycaly. The relative radioactivity was determined by a scintillation radiometer. The data, estimated statistically, on the studies showed that 48 hours after lazer irradiation the incorporation of glycine was elevated over 12%. There were no apparent changes macroscopicaly and in the usual histologic preparations during the same period of time. Electronmicroscopic investigation revealed a certain alteration of the membrane structures, vacuolization at sites, but preserved ribosomal structures, accumulation of glycogen. The established changes were described as a manifestation of nonthermic effects of the type "striking wave" with successive increased course of reparative processes.
D000818:Animals; D005998:Glycine / Q000528:radiation effects; D007834:Lasers; D008099:Liver / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D014176:Protein Biosynthesis; D011506:Proteins / Q000528:radiation effects*; D051381:Rats; D012269:Ribosomal Proteins / Q000528:radiation effects
D011506:Proteins; D012269:Ribosomal Proteins; D005998:Glycine
Vkliuchvane na glitsin(14C) v chernodrobnite proteĭni pri niskoenergiĭno impulsno lazerno luchenie.