Isolation and separation of alpha and beta-tubulin from chick-embryo brain, muscle and skin.
Two kinds of tubulin, alpha and beta, have been described in microtubules from many different systems. In the present study a new method is described for isolating and separating these two kinds of tubulin from chick-embryo brain, muscle and skin. The isolated tubulins were characterized by polyacrylamide-gel disc electrophoresis in the presence of urea and sodium dodecyl sulphate.
D000818:Animals; D001921:Brain / Q000196:embryology; D001923:Brain Chemistry; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D008722:Methods; D009132:Muscles / Q000032:analysis / Q000196:embryology; D012867:Skin / Q000032:analysis / Q000196:embryology; D014404:Tubulin / Q000302:isolation & purification*
D006023:Glycoproteins; D014404:Tubulin