Prostaglandin E and mitogenic stimulation of human lymphocytes in serum-free medium.
Sera used in cell cultures contain significant ammounts of prostaglandins (PGs). In order to avoid any effects of contaminating PGs, the present study employed a serum-free culture medium and confirmed the inhibitory effect of prostaglandin E (PGE) on the human lymphocyte activation which had been observed previously employing a serum-containing medium. PGE1 displayed a significantly stronger inhibitory effect on the cells than previously shown. Furthermore, reported enhancement of PGE synthesis by mitogen-activated lymphocytes could not be reproduced.
D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000378:metabolism*; D011458:Prostaglandins E / Q000096:biosynthesis / Q000494:pharmacology*
D003470:Culture Media; D037102:Lectins; D011458:Prostaglandins E