Disulphates of 16-oxygenated ketonic C19 steroids as biliary metabolities of androsterone sulphate in female rats.
The chemical synthesis of 16beta-hydroxyandrosterone was described preparatory to studies of the disulphates of the 16-oxygenated ketonic C19 steroids present in the bile of female rats dosed with [3H]androsterone sulphate. The biliary metabolites were separated by chromatography on Sephadex LH-20 to afford monosulphate and diconjugate fractions. After solvolysis of the diconjugate fraction, the liberated steroids were separated by partition chromatography on Celite 545 and analyzed by gas chromatography-mass spectometry. In addition to 3alpha, 17beta-dihydroxy-5alpha-androstan-16-one isolated previously, 16beta-hydroxyandrosterone was identified as a disulphate.
D000738:Androsterone / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D001646:Bile / Q000378:metabolism*; D005260:Female; D051381:Rats
D000738:Androsterone