An improved method for detection and titration of antibodies cytophilic for macrophages.
A new microtechnique for detection and titration of antibodies cytophilic for homologous macrophages is introduced and experiments designed to determine the optimum conditions for its performance reported. The technique employs a series of small chambers formed on a microscope slide by the application of a plastic film in which holes have been punched. The procedure uses 5 x 10(4) cells and 25 microliter of serum per chamber, and each test can be completed in 2 h. A permanent preparation results which can be examined as and when convenient.
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006168:Guinea Pigs; D008264:Macrophages / Q000276:immunology*; D012397:Rosette Formation; D013997:Time Factors
D000906:Antibodies