Conformation of methylated sequences in HeLa cell 18-S ribosomal RNA: nuclease S1 as a probe.
18-S rRNA from HeLa cells was digested with nuclease S1. Under the conditions employed 15% of the total nucleotides and some 50% of the methylated nucleotides were released as low-molecular-weight products. The material which was precipitable by 70% ethanol after nuclease S1 digestion was subjected to further digestion by combined T1 plus pancreatic ribonucleases or by T1 ribonuclease alone, and fingerprints were prepared. It was found that the four sites which are modified late during ribosome maturation, and which contain base modifications, were all accessible to nuclease S1. By contrast fewer than one-half of the sites which are modified early during ribosome maturation, and which contain 2'-O-methyl groups, were accessible to nuclease S1; the remainder were protected, presumably by secondary or tertiary interactions within 18-S rRNA.
D001483:Base Sequence; D001665:Binding Sites; D006367:HeLa Cells; D008745:Methylation; D009843:Oligoribonucleotides / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis*; D012260:Ribonucleases / Q000378:metabolism*; D012265:Ribonucleotides / Q000032:analysis
D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D012265:Ribonucleotides; D012260:Ribonucleases