Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis.
A new method is described which allows the purification of large quantities of nuclei (germinal vesicles) from Xenopus laevis vitellogenic oocytes of different developmental stages. From the isolated nuclei, purified nucleoli were obtained. The isolated germinal vesicles are transcriptionally active showing endogenous RNA polymerase activity as well as a high level of activity with an exogenously added template.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005260:Female; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014981:Xenopus
D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases