Establishment and characterization of a cell line derived from human colon adenocarcinoma (HuCCL-14).
A continuous human colon carcinoma cell line (HuCCL-14) was established whose cells possess an epithelial-like morphology and are capable of growing in soft agar and on monolayers of normal cells. HuCCL-14 cells yielded high levels of carcinoembryonic antigen, and the analysis of their chromosomal constitution revealed a mode of 70 chromosomes per cell. HuCCL-14 cells produce malignant tumors when injected into nude mice. Preliminary virologic studies indicate the release of RNA particles having a density of 1.15--1.19 g/ml.
D000230:Adenocarcinoma; D000818:Animals; D002272:Carcinoembryonic Antigen; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D002875:Chromosomes; D002999:Clone Cells / Q000166:cytology; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000209:etiology; D009858:Oncogenic Viruses; D012367:RNA, Viral; D012811:Sigmoid Neoplasms
D002272:Carcinoembryonic Antigen; D012367:RNA, Viral