Methodical investigation of the production of antibodies towards 3,4-dimethoxyphenylethylamine.
In this study the synthesis of various antigens from 3,4-dimethoxyphenylethylamine (3,4-DMPEA) is described. Antigen A was formed by coupling the acylated side chain of 3,4-DMPEA to the protein, while antigen B was synthesized by introducing an acylated amino group to the benzene ring and linking this reactive group to the protein. With antigen A an antiserum with a titer of 1 : 16000 could be raised, while with antigen B coupled to human albumin and bovine gamma-globulin the antiserum had a titer of 1 :1000 and 1 : 4000. Antibodies formed by immunization with antigen A exhibited a high specificity for substituents of the benzene ring, but were less specific for substituents of the side chain. Antibodies formed by treatment with the antigen B were specific for both kinds of substituents. So far, it has not been possible to harvest any specific antibodies towards the corresponding antigens of dopamine.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000276:immunology; D004118:Dimethoxyphenylethylamine / Q000276:immunology*; D005260:Female; D006241:Haptens; D010627:Phenethylamines / Q000276:immunology*; D011817:Rabbits
D002352:Carrier Proteins; D004118:Dimethoxyphenylethylamine; D006241:Haptens; D010627:Phenethylamines