Liquid chromatographic analysis of sebum lipids and other lipids of medical interest.
A technique is described for the high-pressure liquid chromatographic (HPLC) analysis of sebum lipid classes. The lipid present in sebum are separated by gradient elution HPLC from a microparticulate silica column and detected using a moving-wire detector. The system described can be linked to a computer. Quantitation can be carried out by comparing peak areas obtained with those of an internal standard. Peak trapping for further investigations of the separated components, for example by gas chromatography-mass spectrometry, is very easy. Sebum lipids are separated into the following lipid classes: hydrocarbons and squalene, cholesterol esters and wax esters, fatty acids as their methyl esters, triglycerides, 1,3-diglycerides, 1,2-diglycerides, free cholesterol, monoglycerides and other polar materials. Besides to sebum, the method has been successfully applied to other lipid mixtures, such as serum lipids. Examples of other applications are shown.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008055:Lipids / Q000032:analysis* / Q000097:blood / Q000145:classification; D012629:Sebum / Q000032:analysis*; D012997:Solvents
D008055:Lipids; D012997:Solvents