A heterologous radioimmunoassay method for measurement of hamster growth hormone.
A heterologous radioimmunoassay (RIA) for determination of hamster growth hormone (GH) is described which involves the use of radioiodinated rat GH (NIAMDD Rat GH-I-2), antiserum to rat GH (NIAMDD Anti-Rat-GHS-3), a standard hamster anterior pituitary homogenate (SHAP), as well as purified hamster GH (AFP-1595-B). This RIA was shown to be a reliable, sensitive, and specific method for measurement of hamster GH. With this method we have determined the effects of stress and hypophysectomy on serum GH concentrations as well as changes in serum GH concentration, and pituitary GH content and concentration as a function of hamster age. In contrast to responses noted in rats, the stress of ether anesthesia and i.c. puncture caused significant increases in serum GH. A decrease in the serum GH concentration was observed in hamsters at the age 2 to 16 days. Pituitary GH content and concentration increased steadily between 9 and 120 days of age.
D000367:Age Factors; D000375:Aging; D000769:Anesthesia, Inhalation; D000818:Animals; D006224:Cricetinae; D004986:Ether; D005260:Female; D013006:Growth Hormone / Q000097:blood*; D007016:Hypophysectomy; D008297:Male; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000033:anatomy & histology / Q000502:physiology; D011863:Radioimmunoassay / Q000379:methods*; D013045:Species Specificity; D013312:Stress, Physiological / Q000097:blood
D004986:Ether; D013006:Growth Hormone