Radioaffinity assay, a new approach to binding assays applied to the measurement of serum albumin.
The affinity adsorbent Cibacron Blue F3GA-Sepharose 4-B has been used to develop a binding assay for human serum albumin. The method is simple, accurate and precise. It agrees well with the 'rocket' immunoelectrophoretic method and is proposed as an alternative technique for the re-estimation of albumin levels in the low range (less than 30 g/l) where the bromocresol green dye-binding method overestimates. Bilirubin, several drugs, gammaglobulin, haemoglobin and heparin do not interfere with the estimations.
D001961:Bromcresol Green; D004396:Coloring Agents; D005069:Evaluation Studies as Topic; D006801:Humans; D007122:Immunoelectrophoresis; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis*; D012711:Serum Albumin, Radio-Iodinated
D004396:Coloring Agents; D012709:Serum Albumin; D012711:Serum Albumin, Radio-Iodinated; D001961:Bromcresol Green