The effect of refreezing previously frozen-deglycerolized red blood cells.
Frozen red blood cells, once thawed and washed, must be used within 24 hours or discarded. In this study, three units of blood underwent two freeze-thaw cycles. It was shown that red blood cell survival, ATP, and 2,3-DPG were not adversely effected. The data suggest that previously frozen red blood cells should be refrozen, rather than discarded, if not used within 24 hours of deglycerolization.
D000255:Adenosine Triphosphate / Q000097:blood; D004163:Diphosphoglyceric Acids / Q000097:blood; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000502:physiology*; D005615:Freezing; D005990:Glycerol / Q000378:metabolism*; D006801:Humans; D013997:Time Factors
D004163:Diphosphoglyceric Acids; D000255:Adenosine Triphosphate; D005990:Glycerol