Toxic and transforming effects of polycyclic hydrocarbons in fetal hamster lung-cell cultures. I. Benzo(alpha)pyrene.
When seeded at a low cell density in Petri dishes not pre-conditioned with feeder layers, fetal hamster lung cells at 2-4 passages grew with greatly varied plating efficiencies (PE). Addition of insulin to the serum-supplemented medium gave relatively constant PE. However, the presence of insulin in the medium enhanced the toxicity of benzo(alpha)pyrene (BP) on these cells. Cytotoxic and cell transforming effects of BP in the presence of insulin were studied.
D001580:Benzopyrenes / Q000633:toxicity*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002999:Clone Cells; D007328:Insulin / Q000494:pharmacology
D001580:Benzopyrenes; D007328:Insulin