An improved screening method for 3-methoxy-4-hydroxy mandelic acid excretion in urine.
A screening method is described for urinary 3-methoxy-4-hydroxy mandelic acid using an initial ion exchange procedure, vanillin formation and extraction into toluene. The technique, is simple, rapid and specific, with a reference value up to 35 mumol (7.0 mg)/day established on 92 normal human subjects. Over 200 patients' urines were analysed by the method of which 18 gave values above the reference range. The majority of these increased excretions are attributed to the metabolic effects of drugs, one patient suffered from phaeochromocytoma and only 2 results remaining unexplained.
D002852:Chromatography, Ion Exchange; D005421:Flavoring Agents / Q000096:biosynthesis; D006801:Humans; D007202:Indicators and Reagents; D008333:Mandelic Acids / Q000652:urine*; D010673:Pheochromocytoma / Q000175:diagnosis*
D005421:Flavoring Agents; D007202:Indicators and Reagents; D008333:Mandelic Acids