Mutagenic effect of orally given AF-2 on embryonic cells in pregnant Syrian hamsters.
Pregnant hamsters were given various doses of AF-2 by stomach tube; then the cells of their embryos were isolated and cultured in normal medium. Chromosome preparations were made within 24 h after the start of primary culture, and examined for chromosomal aberrations. Marked chromosomal abnormalities were observed in cells of embryos of animals treated with AF-2 at over 20 mg/kg. Samples of surviving cells were also cultured in normal medium for 48 h, and then selected in medium containing 8AG or 6TG. This treatment with AF-2 caused marked dose-dependent induction of 8AG- or 6TG-resistant mutations: mutant colonies were even obtained after a single treatment with 2 mg of AF-2 per kg. These results show that this is a sensitive and useful mammalian system for detecting environmental mutagens.
D000284:Administration, Oral; D000818:Animals; D001375:Azaguanine / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000187:drug effects*; D005260:Female; D005520:Food Preservatives / Q000009:adverse effects*; D005668:Furylfuramide / Q000008:administration & dosage / Q000009:adverse effects*; D009154:Mutation; D009581:Nitrofurans / Q000009:adverse effects*; D011247:Pregnancy; D013866:Thioguanine / Q000494:pharmacology
D005520:Food Preservatives; D009581:Nitrofurans; D005668:Furylfuramide; D013866:Thioguanine; D001375:Azaguanine