Physical properties and Tris inhibition of an insect trehalase and a thermodynamic approach to the nature of its active site.
The midgut from Rhynchosciara americana larvae display a trehalase (alpha,alpha'-trehalose glucohydrolase, EC 3.2.1.28) which is soluble with a molecular weight of 122 000 and pI 4.6. The optimum pH of the enzyme is 6.0, its apparent Km for trehalose is 0.67 mM and its energy of activation is 16.7 kcal/mol. Sulfhydryl reagents do not inhibit the trehalase. The results suggest the existence of two carboxyl groups in the active site, one of which has a very high (8.3) pK. The increase of the pK values of the essential groups of the free enzyme in the presence of increasing concentrations of dioxane supports the hypothesis that these groups are carboxyls. The purified enzyme hydrolyzes only alpha,alpha'-trehalose and it is competitively inhibited by several compounds.
D000818:Animals; D001665:Binding Sites; D004064:Digestive System / Q000201:enzymology; D004175:Diptera / Q000201:enzymology*; D005260:Female; D007700:Kinetics; D007814:Larva; D008970:Molecular Weight; D013379:Substrate Specificity; D013816:Thermodynamics; D014198:Trehalase / Q000302:isolation & purification / Q000378:metabolism; D014325:Tromethamine / Q000494:pharmacology
D014325:Tromethamine; D014198:Trehalase