[Incorporation of radioactive protein and RNA precursors into animal liver polysomes under hydroxythiamine injection].
Effect of hydroxythiamine, B1 antivitamin, on the incorporation of labelled protein precursor (14C-hydrolysate of chlorella protein, 14C-leucine) and 14C-orotic acid into polysomes and RNA fractions of rat and mouse liver is investigated. Single subcutaneous injection of hydroxythiamine (400 mg/kg) is found to inhibit in 24 hours the synthesis of 28S and 18S rRNAs, polysome profile being unchanged and the incorporation of the label into protein being increased. Hydroxythiamine is proposed to induce the synthesis of TDP-dependent enzyme proteins responsing the decrease of their activity.
D000818:Animals; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D009963:Orotic Acid / Q000378:metabolism*; D010119:Oxythiamine / Q000494:pharmacology*; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D051381:Rats; D013844:Thiazoles / Q000494:pharmacology*
D011506:Proteins; D012335:RNA, Ribosomal; D013844:Thiazoles; D010119:Oxythiamine; D009963:Orotic Acid; D007930:Leucine
Vkliuchenie radioaktivnykh predshestvennikov belka i RNK v polisomby pecheni zhivotnykh pri vvedenii oksitiamina.