A new approach to the autoradiographic study of proliferating lymphocytes.
An adaptation of a cell filtration method for the autoradiographic study of cultured lymphocytes has been developed. The percentages of labelled cells are very similar in the filtered cell population to those obtained from replicate cultures processed by centrifugation. The filter method permitted a higher recovery of cells from small numbers in culture with better cytological detail than seen when cells were washed by repeated centrifugation, then suspended and smeared.
D001345:Autoradiography; D002455:Cell Division; D002478:Cells, Cultured; D005374:Filtration / Q000379:methods; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*