Electrophoresis of cells at the interface liquid/liquid.
A new method for measuring the mobility of cells in an electric field is described. The movement of cell samples is carried out at the interface: isotonic phosphate buffer (pH 7.37, mu = 2.214) with Ficoll--the same phosphate buffer without Ficoll. After the voltage is switched on, cells are moving in parallel along the interface. The movement is accomplished by sliding on the denser of these two layers in a flat cuvete. Several samples of cells may be applied and their relative displacement compared. The method was established for human erythrocytes and tannined human erythrocytes.
D002469:Cell Separation / Q000379:methods*; D004586:Electrophoresis / Q000379:methods; D004912:Erythrocytes; D006801:Humans