Activity and polymorphism of the cobalt-activated acylase in tissues of rodents during development.
The activity of cobalt-activated acylase, measured towards N-chloroacetyl- and N-butyryl-gamma-L-glutamyl-beta-naphthylamide, was found in all tissues of the adult animals. In the kidney, liver and small intestine of adult guinea-pig and rat two fractions differing in electrophoretic mobility (fractions 1 and 2) were present. The early foetus contained fraction 2, sometimes accompanied by fraction 3 which later disappeared; on further development of the foetus, fraction 1 appeared. Fraction 1 was distinctly activated by cobalt ions; fractions 2 and 3 were strongly inhibited by deaminated leucylphenylalanine. In the guinea-pig, the molecular weight of the three fractions ranged from 43000 to 59000.
D000818:Animals; D003035:Cobalt; D006224:Cricetinae; D003641:Deamination; D004789:Enzyme Activation; D005333:Fetus / Q000201:enzymology*; D006168:Guinea Pigs; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D051379:Mice; D008970:Molecular Weight; D011110:Polymorphism, Genetic; D011817:Rabbits; D051381:Rats
D003035:Cobalt