Neuronal adjustments in developing nuclear centers of the chick embryo following transplantation of an additional optic primordium.
Following transplantation of an additional optic primordium into the orbital mesenchyme of chick embryos of approximately 2 days of incubation age, the changes in cell number in the ciliary ganglion, accessory oculomotor and trochlear nuclei were studied at various stages of development. Cell counts were made at 1-day intervals from days 9 through 15 for ciliary ganglion, and from days 13 through 15 for the accessory oculomotor and trochlear nuclei. Cell counts for the ciliary ganglion on days 9 and 11 were similar on the operated and control sides which suggests that grafting of an additional optic primordium, and thus enlarging the periphery, is not involved in the control of proliferation. Comparison of the number of cells for the ciliary ganglia and the accessory oculomotor nuclei at days 13 and 15 showed an increase on the affected side ranging from 8 to 27%, and 9 to 33% respectively. We interpret this increase on the experimental side as a reduction in the number of degenerating cells that occur in normal development, as a result of an enlargement of the peripheral field of innervation. Three cases showed an increase in the number of cells in the trochlear nucleus ranging from 9 to 29%. This increase was attributed to an increase in the size of the superior oblique muscle of the operated side as determined by volumetric measurements. On the basis of the evidence we conclude that an enlarged periphery acts by regulating the level of naturally occurring cell death by reducing the amount of cell loss, leading to a corresponding increase in final cell number.
D000818:Animals; D002452:Cell Count; D002470:Cell Survival; D002642:Chick Embryo; D002924:Ciliary Body / Q000166:cytology / Q000196:embryology* / Q000294:innervation; D005123:Eye / Q000196:embryology / Q000637:transplantation; D005725:Ganglia, Autonomic / Q000166:cytology / Q000196:embryology; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology*; D009474:Neurons / Q000166:cytology*; D009802:Oculomotor Nerve / Q000166:cytology / Q000196:embryology; D013477:Superior Colliculi / Q000166:cytology / Q000196:embryology*; D014184:Transplantation, Homologous; D014321:Trochlear Nerve / Q000166:cytology / Q000196:embryology