Fluorinated molecule as a tracer: difluoroserotonin in human platelets mapped by electron energy-loss spectroscopy.
The intracellular distribution of fluorine has been delineated in human platelets incubated with 4,6-difluoroserotonin, utilizing a scanning-transmission electron microscope equipped with an energy-loss spectrometer. Discrete intracellular structures corresponding in location to dense bodies contained high concentrations of fluorine. Electron energy-loss spectroscopy, which apparently can detect less than 10(-20) gram of fluorine in an area of 10 square nonometers, can thus localize fluorinated tracer molecules with biological activity.
D001792:Blood Platelets / Q000378:metabolism*; D004735:Energy Transfer; D005461:Fluorine; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods; D012701:Serotonin / Q000031:analogs & derivatives* / Q000097:blood; D013057:Spectrum Analysis / Q000379:methods
D005461:Fluorine; D012701:Serotonin; C016378:4,6-difluoroserotonin