Antigen shedding by human breast-cancer cells in vitro and in vivo.
Human breast cancer cells were adapted to chemically defined medium in order to recover naturally shed glycoproteins. Sephadex G-150 chromatography of these glycoproteins revealed 1 major and 2 minor peaks. When the cells were grown in the presence of 3H-leucine, the radioactive shed proteins had a Sephadex profile identical to the unlabelled shed glycoproteins. PAGE analysis of these proteins showed 5 major bands. Antigenically similar proteins were found in the serum of female nude mice bearing BOT-2 tumours, but not in controls.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D001943:Breast Neoplasms / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000276:immunology; D051379:Mice
D000951:Antigens, Neoplasm; D006023:Glycoproteins