Oestradiol-receptor complexes in subnuclear fractions of rat uterine tissue.
The subnuclear distribution of 3H-oestradiol-receptor complexes was studied in uterine tissue of ovariectomized adult rats. Nuclei were sonically disrupted and 8 different subnuclear fractions were isolated by discontinuous sucrose density gradient centrifugation. 3H-Oestradiol-receptor complexes, measured by hydroxylapatite column chromatography, were localized in a light chromatin fraction as well as in a heavy chromatin fraction. Using the hydroxylapatite chromatography technique it was possible to demonstrate three classes of oestradiol-receptor complexes which differ in affinity for the chromatin. Oestradiol-receptor complexes with a high affinity for the chromatin were predominantly localized in the heavy chromatin fraction, whereas complexes with a lower affinity for their acceptor sites were present in the lighter chromatin fraction.
D000818:Animals; D002369:Castration; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006570:Heterochromatin / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D011960:Receptors, Estrogen / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism* / Q000648:ultrastructure
D002843:Chromatin; D006570:Heterochromatin; D011960:Receptors, Estrogen; D004958:Estradiol; D012313:RNA