A simple and specific competitive protein binding assay for 24,25-dihydroxyvitamin D in human serum.
A sensitive and specific competitive protein binding assay for 24,25-dihydroxyvitamin D was developed using rat kidney cytosol as the binding protein. It also included a chromatographic step on a Sephadex LH 20 column to separate the metabolite specifically. The average value for 24, 25-dihydroxyvitamin D in serum in 19 male and female healthy subjects aged from 20 to 40 was found to be 8.24 +/- 0.34 ng/ml (1.98 +/- 0.08 . 10(-8) mol/l) mean +/- S.D. In hepatic insufficiencies the levels are higher (14.15 +/- 3.59 ng/ml) than in normal subjects. A comparison between 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D levels was made.
D000328:Adult; D001667:Binding, Competitive; D003600:Cytosol / Q000378:metabolism; D004100:Dihydroxycholecalciferols / Q000097:blood*; D005260:Female; D006801:Humans; D006887:Hydroxycholecalciferols / Q000097:blood*; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008297:Male; D011485:Protein Binding
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols