Alteration of brain chromatin and nuclear synthetic activity in morphine-tolerant rats.
3H-UTP incorporation by endogenous RNA-polymerase of intact, isolated rat brain nuclei was enhanced by chronic morphine treatment. Analgesic tolerance using the hot-plate assay was also evident. Fractionation proflies for brain chromatin on hydroxylapatite from morphine and vehicle-treated rats was different. These results suggest that morphine-tolerance in the rat may be accompanied by enhanced nuclear synthesis of a new species of RNA.
D000698:Analgesia; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000378:metabolism*; D002843:Chromatin / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D004361:Drug Tolerance; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D011489:Protein Denaturation; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D013816:Thermodynamics
D002843:Chromatin; D012313:RNA; D009020:Morphine