[Intracellular structures of influenza virus].
The intracellular influenza virus-containing structures involved in RNA synthesis in the cytoplasm and in the nucleoplasm of infected chicken fibroblasts were studied. Two approaches were used: (1) short pulse labeling of infected cell with [3H]uridine; (2) determination in vitro of polymerase activity of intracellular virus-specific structures. Both methods revealed functionally active virus-specific structures in the nucleoplasm and showed that a functionally active virus-specific structure was localized in the nucleoplasm of infected cells. This structure contained proteins of the viral ribonucleoprotein, but sedimented somewhat faster (at 60--90S in velocity sucrose and glycerol gradients). Meanwhile, polymerase-containing structures in the cytoplasm of infected cells sedimented in the position of viral ribonucleoproteins (25--60S).
D002467:Cell Nucleus / Q000201:enzymology / Q000382:microbiology; D002478:Cells, Cultured; D003593:Cytoplasm / Q000382:microbiology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D009980:Influenza A virus / Q000032:analysis / Q000201:enzymology / Q000648:ultrastructure*; D014529:Uridine / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis*
D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases; D014529:Uridine
Vnutrikletochnye struktury virusa grippa.