Rapid reversed-phase high-performance liquid chromatographic analysis of steroid products.
The optimisation of conditions for the application of reversed-phase high-performance liquid chromatography (HPLC) to the analysis of steroid products is discussed. The influence of temperature and flow-rate on column efficiency are described. At 60 degrees, the reduced velocity required to give maximum efficiency, is approximately twice that found at room temperature, allowing faster through-put. (hv) curves are reported for 5-micron Spherisorb-ODS and Hypersil-ODS material for corticosteroids at 60 degrees. On-column injection techniques are discussed and data presented, showing that for corticosteroids on-column loop injection can be efficient as septum injection using the techniques described.
D001623:Betamethasone / Q000031:analogs & derivatives / Q000032:analysis; D001624:Betamethasone Valerate / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002990:Clobetasol / Q000032:analysis; D004304:Dosage Forms; D013259:Steroids, Fluorinated / Q000032:analysis*
D004304:Dosage Forms; D013259:Steroids, Fluorinated; D001623:Betamethasone; D001624:Betamethasone Valerate; D002990:Clobetasol