Effect of a choline inhibitor (N-isopropylethanolamine) on cellular metabolism of L-M cells.
N-Isopropylethanolamine, a choline analog, is incorporated into L-M cell lipids as 1,2-diacyl-sn-glycero-3-phosphoisopropylethanolamine de novo and not by base exchange. In addition, the N-isopropylethanolamine effectively blocks choline uptake, which is a reversible process. The following specific time-dependent changes in cell metabolism also occur when N-isopropylethanolamine is present: (1) a decrease in total content of phosphatidylcholine, (2) inhibition of both the cellular uptake of [3H]choline and its incorporation into phosphatidycholine, (3) a decrease in the incorporation of [3H]thymidine into DNA as early as 2 h after initiating the N-isopropylethanolamine block, (4) inhibition of the cellular uptake of [3H]uridine and incorporation into RNA 16--24 h after addition of the N-isopropylethanolamine, and (5) stimulation of the cellular uptake of [3H]leucine and an inhibition of its incorporation into protein, which reached a maximum (68% of controls) 8 h after N-isopropylethanolamine treatment.
D002478:Cells, Cultured; D002794:Choline / Q000037:antagonists & inhibitors*; D004247:DNA / Q000096:biosynthesis*; D004983:Ethanolamines / Q000378:metabolism / Q000494:pharmacology*; D008861:Microsomes / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D011437:Propylamines / Q000378:metabolism / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D013997:Time Factors
D004983:Ethanolamines; D010743:Phospholipids; D011437:Propylamines; D012313:RNA; D004247:DNA; D002794:Choline