Distortion of endothelial repair. The effect of hypercholesterolaemia on regeneration of aortic endothelium following injury by endotoxin. A scanning electron microscope study.
Five young male New Zealand White rabbits were fed a semi-synthetic diet containing 0.2% cholesterol for 2 weeks and a control group of 5 animals was fed a normal stock diet. All animals were then injected intravenously with a single dose of endotoxin from Serratia marcescens (200 microgram/kg body weight) and continued on their respective diets for a further 4 weeks. The aortas were then stained with silver nitrate and fixed under pressure for Scanning Electron Microscopy (SEM). Argyrophilic endothelial cells were present in both groups of animals 4 weeks after endotoxin injections. In the cholesterol-fed animals, however, these cells were often covered with pits and craters. These findings suggest that the hypercholesterolaemia may affect the regeneration of arterial endothelial cells.
D000818:Animals; D001011:Aorta / Q000293:injuries* / Q000503:physiopathology; D001018:Aortic Diseases / Q000473:pathology* / Q000503:physiopathology; D002791:Cholesterol, Dietary; D004727:Endothelium / Q000473:pathology / Q000503:physiopathology; D004731:Endotoxins; D006937:Hypercholesterolemia / Q000503:physiopathology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D012038:Regeneration
D002791:Cholesterol, Dietary; D004731:Endotoxins