[Synchronous growth in liquid medium of species related to "Mycobacterium rhodochrous": "Nocardia restricta" and "Jensenia canicruria" (author's transl)].
The synchronous development of nocardioform and coryneform populations had not yet been studied. We have observed that it is possible to obtain a synchronous growth of Nocardia restricta and Jensenia canicruria (species related to Mycobacterium rhodochrous) in a liquid synthetic medium by inoculating the medium with spherical cells taken during the advanced stationary phase, at the end of the cell division cycle. Good synchrony produces a series of breaks in the slope of culture absorbance and of cell protein content. DNA replication checked either by cell DNA content measurements or by pulse-incorporations of tritiated thymidine, is discontinous; peaks of incorporation of thymidine follow one another with a periodicity equal to the generation time. A study of the cell division cycle, hitherto unknown, is now possible.
D001426:Bacterial Proteins / Q000096:biosynthesis; D002455:Cell Division; D003470:Culture Media; D004261:DNA Replication; D004269:DNA, Bacterial; D009161:Mycobacterium / Q000254:growth & development* / Q000378:metabolism; D009615:Nocardia / Q000254:growth & development* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D013936:Thymidine
Croissance synchrone en milieu liquide d'espèces liées à Mycobacterium rhodochrous: Nocardia restricta et Jensenia canicruria.