Damage of DNA and its recovery in AH-109A cells treated with carboquone in vivo.
A single injection of carboquone at a dose of 0.1 mg/kg body weight induced damage of DNA of AH-109A cells as revealed by alkaline and neutral sucrose density gradient centrifugation. A significant decrease in the sedimentation velocity of DNA on alkaline sucrose density gradient centrifugation was observed 15 min after the injection, and it returned to that of control after 60 min. Thereafter, the size of DNA decreased progressively. When the cells were lysed with 2% sodium dodesyl sulfate solution and analyzed on neutral sucrose density gradient centrifugation, a similar change in the sedimentation velocity was observed. The results obtained from the studies of intraperitoneal growth of AH-109A cells pretreated with carboquone in vivo and the survival of host animals well corresponded to the extent of the damage of DNA as revealed by alkaline and neutral sucrose density gradient centrifugation.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001389:Azirines / Q000494:pharmacology*; D002225:Carbazilquinone / Q000494:pharmacology*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004273:DNA, Neoplasm / Q000032:analysis; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D051381:Rats
D000970:Antineoplastic Agents; D001389:Azirines; D004273:DNA, Neoplasm; D002225:Carbazilquinone