Enzymes from Micrococcus luteus involved in the initial steps of excision repair of spontaneous DNA lesions: uracil-DNA-glycosidase and apurinic-endonucleases.
Uracil-DNA-glycosidase that releases free uracil from single-stranded or double-stranded deaminated DNA and poly d(A-U) has been partially purified from Micrococcus luteus. The enzyme has a molecular weight of about 16,000 and can be separated from uracil-endonuclease and endonucleases (AP-endonucleases) specific for apurinic and apyrimidinic sites. Uracil-DNA-glycosidase does not act on guanine residues opposite uracil in double-stranded DNA and on xanthine in deaminated DNA. The glycosidase generates apyrimidinic sites which can serve as substrate sites for different AP-endonucleases from M. luteus.
D001080:Apurinic Acid; D001665:Binding Sites; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides; D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism*; D008837:Micrococcus / Q000201:enzymology*; D009699:N-Glycosyl Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D011089:Polydeoxyribonucleotides / Q000378:metabolism
D004269:DNA, Bacterial; D003856:Deoxyuracil Nucleotides; D011089:Polydeoxyribonucleotides; D001080:Apurinic Acid; D003851:Deoxyribonucleases; D004720:Endonucleases; D009699:N-Glycosyl Hydrolases