On use of chromogenic substrates for studies of coagulation inhibitors.
Methodological problems encountered using chromogenic substrates on thrombin and Xa are discussed: (1) influence of substrate on inhibitor; (2) influence of heparin; (3) optimal concentrations; (4) specificity; (5) denatured enzymes, and (6) natural versus chromogenic substrates.
D000991:Antithrombins / Q000378:metabolism; D001777:Blood Coagulation; D006493:Heparin / Q000494:pharmacology; D013379:Substrate Specificity; D013917:Thrombin / Q000378:metabolism
D000991:Antithrombins; D006493:Heparin; D013917:Thrombin