Immunological specificity of anti-zona binding to zona pellucida.
Biochemically purified and F(ab')2 immunoglobulin fractions were prepared from several antisera, including a human serum, which contained anti-zona pellucida activity. Anti-zona activity was monitored either by the formation of the antibody produced precipitation layer on the zona following antiserum treatment and/or by the indirect fluorescent antibody method. No changes in anti-zona activity were observed when the purified and the digested immunoglobulin preparations from each antiserum were compared to their whole serum counterparts. It is included that anti-zona binding to the zona as well as the formation of the precipitation layer on the zona is the result of a specific antigen-antibody reaction.
D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D010063:Ovum / Q000276:immunology*; D011817:Rabbits; D013552:Swine; D015044:Zona Pellucida / Q000276:immunology* / Q000648:ultrastructure
D007140:Immunoglobulin Fab Fragments