Oxidation of 3beta-hydroxyandrostenes by the 3beta-hydroxy-steroid oxidase (cholesterol oxidase) from Brevibacterium sterolicum prior to their analysis by gas-liquid chromatography-mass spectrometry.
The 3beta-hydroxysteroid oxidase from Brevibacterium sterolicum has been applied to the oxidation of a number of 3beta-hydroxyandrostenes, including polar steroids containing up to three other hydroxylic groups. The substrates, products, and derivatives thereof have been examined by gas-liquid chromatography. Retention index increments for these conversions, and for parallel transformations of other steroids, show considerable regularities, and together with mass spectrometric data afford characteristic structural information.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000737:Androstenols / Q000032:analysis*; D001951:Brevibacterium / Q000201:enzymology*; D002789:Cholesterol Oxidase / Q000378:metabolism*; D002849:Chromatography, Gas; D013058:Mass Spectrometry
D000737:Androstenols; D015096:3-Hydroxysteroid Dehydrogenases; D002789:Cholesterol Oxidase