Metabolism of tritium-labeled 12-O-tetradecanoylphorbol-13-acetate by cells in culture.
The metabolism of [20-3H]-12-O-tetradecanoylphorbol-13-acetate ([3H]TPA) was studied in human and hamster cell cultures. Within 2 to 3 days after its addition to growing or confluent cultures of hamster embryo fibroblasts, no unchanged [3H]TPA remained in the medium as determined by thin-layer chromatography of the chloroform phase obtained by extraction of the medium with chloroform:methanol:H2O. In contrast, little or no metabolism of [3H]TPA occurred under identical conditions in cultures of human fibroblasts. The major metabolite formed from [3H]TPA in hamster cell cultures was [3H]phorbol-13-acetate. with both hamster and human cells, virtually all cell-associated radioactivity was unchanged [3H]TPA.
D000818:Animals; D002478:Cells, Cultured; D002725:Chloroform; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D010703:Phorbol Esters / Q000378:metabolism; D010704:Phorbols / Q000378:metabolism*; D013045:Species Specificity; D013755:Tetradecanoylphorbol Acetate / Q000032:analysis / Q000378:metabolism*; D014316:Tritium
D010703:Phorbol Esters; D010704:Phorbols; D014316:Tritium; D002725:Chloroform; D013755:Tetradecanoylphorbol Acetate