Simple and rapid high-pressure liquid chromatographic simultaneous determination of aspirin, salicylic acid, and salicyluric acid in plasma.
A rapid and sensitive high-pressure liquid chromatographic assay was developed for aspirin, salicylic acid, and salicyluric acid in plasma. The procedure involves the solvent extraction of these compounds from plasma and separation using a reversed-phase column eluted by acidified aqueous acetonitrile. Small quantitites of aspirin can be assayed directly in the presence of a large quantity of salicylic acid. The assay is also free from blank interference.
D001241:Aspirin / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006626:Hippurates / Q000097:blood*; D008722:Methods; D012459:Salicylates / Q000097:blood*; D013997:Time Factors
D006626:Hippurates; D012459:Salicylates; C000693:salicylurate; D001241:Aspirin