title: Rapid accumulation of vitellogenin messenger RNA during secondary estrogen stimulation of Xenopus laevis.
abstract: Accurate quantitation of low concentrations of vitellogenin mRNA by hybridization to vitellogenin cDNA allows analysis of the accumulation of new vitellogenin mRNA sequences throughout secondary estrogen stimulation. Administration of a secondary injection of estradiol-17 beta to male Xenopus laevis which have been withdrawn from estrogen for 60 days results in synthesis of complete vitellogenin mRNA molecules in as little as 1 h after restimulation. Vitellogenin mRNA accumulates at a rate of 13 molecules/cell/min--at least four times the rate observed in primary estrogen stimulation and peaks at a level twice that observed in primary stimulation. Administration of estrogen to male Xenopus laevis evokes stable long lived changes in the pattern of vitellogenin gene expression and constitutes a type of cellular "memory effect."
mesh_terms: D000818:Animals; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005796:Genes; D008074:Lipoproteins / Q000096:biosynthesis*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*; D013997:Time Factors; D014819:Vitellogenins / Q000096:biosynthesis*; D014981:Xenopus / Q000378:metabolism*
chemical_list: D008074:Lipoproteins; D012333:RNA, Messenger; D014819:Vitellogenins; D004958:Estradiol