Biochemical markers of cultured human prostatic epithelium.
Medium from primary cultures of human prostatic cells was examined for polyamines and carcinoembryonic antigen. Spermine was elevated in medium from 5 of 17 epithelial cell cultures but was not found to be a specific marker for cultured human prostatic epithelial cells. Carcinoembryonic antigen was elevated in all 27 prostate epithelial-cell culture fluids tested, 24 of which had more than 20 ng. carcinoembryonic antigen per milliliter. Medium from other cultured human cells (prostate fibroblasts, genitourinary tumor and non-tumor cells), as well as control medium, contained less than 1 ng. carcinoembryonic antigen per milliliter. Carcinoembryonic antigen, as measured in this study, is a way to demonstrate the presence of human prostatic epithelial cells in culture.
D001322:Autoanalysis; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D004848:Epithelium / Q000032:analysis; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D011073:Polyamines / Q000032:analysis; D011467:Prostate / Q000166:cytology* / Q000378:metabolism; D011700:Putrescine / Q000032:analysis; D011863:Radioimmunoassay; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis
D002272:Carcinoembryonic Antigen; D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine