Identification of a 48 S preinitiation complex in reticulocyte lysate.
When the polypeptide initiation sequence is interrupted by the peptide antibiotic edeine, the sedimentation coefficient of the native small ribosomal subunit is increased from 43 S to 48 S. Formation of the 48 SN particle is absolutely dependent on the binding of both Met-tRNAf and globin mRNA. These results support the concept that Met-tRNAf binds to the native small ribosomal subunit before mRNA, and that processes involved in mRNA binding to the 43 SN preinitiation complex may be the rate-limiting step of 80 S initiation complex formation.
D000818:Animals; D004486:Edeine / Q000494:pharmacology; D007700:Kinetics; D008715:Methionine; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D010448:Peptide Initiation Factors; D012343:RNA, Transfer / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D010448:Peptide Initiation Factors; D004486:Edeine; D012343:RNA, Transfer; D008715:Methionine