The site of 25-hydroxycholecalciferol stimulated protein synthesis in the rat kidney.
Autoradiographs of the kidneys of rachitic rats dosed in vivo with 250 pmoles 25-hydroxycholecaliferol (25-OHD3) and 3H-leucine showed increased grain counts in portions of proximal renal tubules. On incubation of kidney slices the synthesis of only 1 cytosol protein was found to be stimulated by the steroid. On disc gel electrophoresis it had the characteristics of renal calcium binding protein.
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D006887:Hydroxycholecalciferols / Q000494:pharmacology*; D066298:In Vitro Techniques; D007684:Kidney Tubules / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats
D006887:Hydroxycholecalciferols; D007930:Leucine