title: Effect of castration and testosterone administration on the neuromuscular junction in the levator ani muscle of the rat.
abstract: The ultrastructure of the neuromuscular junction (n.m.j.) of the androgen-sensitive levator ani muscle was studied in normal adult male rats, in 8-month-old rats castrated at the age of one month and in castrated rats treated with testosterone propionate (TP). Castration does not result in significant changes of the n.m.j. The density of synaptic vesicles and the postsynaptic junctional folds remain practically normal inspite of marked atrophy of the muscle. TP administration for 7 days results in marked changes in pre- and postsynaptic structures. There is slow progressive depletion of synaptic vesicles, appearance of cisternae and coated vesicles in axon terminals, and coalescence of coated vesicles with the plasma membrane. Coated vesicles are also found inside Schwann cells and among junctional folds. Dense core vesicles appear both in the axon terminals and in the postsynaptic area. Collateral sprouting of terminal axons with the formation of new immature junctions is observed. After 35 days of TP administration depletion of synaptic vesicles continues. Glycogen beta-particles, mostly freely dispersed, occasionally seen in axon terminals 7 days after TP administration, subsequently increase in number. In the endplate zone of the muscle fibre increased protein synthesis is indicated by a rapid increase in ribosomes and irregularly located myofilaments and myofibrils. The appearance of n.m.j. after testosterone administration resembles that described after nerve stimulation; the degree of change is however less pronounced.
mesh_terms: D000818:Animals; D002369:Castration; D006003:Glycogen / Q000032:analysis; D008297:Male; D009132:Muscles / Q000294:innervation*; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000648:ultrastructure; D010502:Perineum / Q000033:anatomy & histology*; D051381:Rats; D013572:Synaptic Vesicles / Q000187:drug effects / Q000648:ultrastructure; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D013739:Testosterone; D006003:Glycogen