title: [Detection of C-strain virus in pigs following vaccination against swine fever (author's transl)].
abstract: During and shortly after a mass vaccination campaign against swine fever with C-strain virus, samples were received from five vaccinated herds, which were found positive on examination with the cryostat immunofluorescent technique (IFT). In four cases fluorescence was due to C-virus antigen as determined by rabbit inoculation. The samples concerned were derived from 4 to 13 week old pigs which had been vaccinated 6 to 7 days prior to death. The fluorescence was notably bright and was confined to the epithelial lining of the tonsillar crypts. The persistence of C-virus following vaccination was studied in twelve-week-old pigs susceptible to swine fever. The animals were immunosuppressed by a concurrent treatment with corticosteroids. Virus antigen was detected by immuno-fluorescence in the tonsils and kidneys till 15 days following vaccination. The replication of virus in these organs is believed to be responsible for the spreading of C-virus observed in the field. Isolation of vaccine virus in rabbits was also possible till 15 days after vaccination. It is concluded that vaccination with C-virus may interfere with the laboratory diagnosis of swine fever by the fluorescent antibody technique applied to tissue sections for at least two weeks.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D006691:Classical Swine Fever / Q000382:microbiology* / Q000517:prevention & control; D012328:RNA Viruses / Q000276:immunology / Q000302:isolation & purification*; D013552:Swine; D014611:Vaccination / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral
vernacular_title: Aantonen van C-virus in varkens na vaccinatie tegen varkenspest.