title: Human serum hemopexin: direct evidence for change of its isoelectric point upon heme binding. A new serum protein fractionation.
abstract: Human serum was submitted to a one step displacement-ligand exchange chromatography. Displacement removed serum albumin and part of gamma-globulins. Ligand exchange furnished an enriched heme-hemopexin fraction. An original, non denaturing human heme-hemopexin preparation is proposed.
mesh_terms: D001798:Blood Proteins / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D006418:Heme; D006466:Hemopexin / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007526:Isoelectric Point; D009994:Osmolar Concentration; D011485:Protein Binding
chemical_list: D001798:Blood Proteins; D006418:Heme; D006466:Hemopexin