[Effect of phloridzin on photosynthetic electron transport].
Phloridzin (2',4',6',4-tetraoxyhydrochalcon-2'-glucoside) was used to study the localization of synthesis of ATP in the electrontransporting chain of photosynthesis. It was shown that phloridzin inhibits the rate of photoreduction of NADP+ by isolated pea chloroplasts by 40%, electron transport via cytochrome f by 100% and via plastocyanin--by 50%. The "crossover" experiments demonstrated that phloridzin inhibits ADP-induced photoreduction of cytochrome f, having no effect on plastocyanin under identical conditions. It is assumed that the site of ATP synthesis is localized on the reduced site of cytochrome f, while the carrier itself is located in the electron transporting chain coupled to phosphorylation. It is possible that only part of the plastocyanin molecules are located in the phosphorylating pathway of electron transport.
D000255:Adenosine Triphosphate / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D004579:Electron Transport; D007700:Kinetics; D010695:Phlorhizin / Q000494:pharmacology*; D010788:Photosynthesis / Q000187:drug effects*; D010944:Plants
D000255:Adenosine Triphosphate; D010695:Phlorhizin
Deistvie floridzina na fotosinteticheskiĭ elektronnyĭ transport.