[Effect of the state of the lipid phase of the membrane on the effectiveness of photochemical modification of erythrocyte acetylcholinesterase].
Modification of the lipid phase structure of the erythrocyte membrane by phospholipases A2, C and D as well as the partial depletion of cholesterol was shown to be accompanied by the change of the acetylcholinesterase (AChE) UV-sensitivity. The ability of UV-light to change the catalytic properties (Km) of the membrane-bound AChE not observed for free AChE (constant value of Km) and known as the phenomenon of photochemical allotopy, is retained in the cholesterol depleted membranes and disappears after an enzymatic treatment of the membranes by phospholipases. The possible non-photochemical influence of the membrane lipid phase in response to UV-damage of membrane-bound AChE is discussed.
D000110:Acetylcholinesterase / Q000528:radiation effects*; D002784:Cholesterol / Q000502:physiology; D002942:Circular Dichroism; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes; D008081:Liposomes; D008563:Membrane Lipids / Q000502:physiology*; D008968:Molecular Conformation / Q000528:radiation effects; D010740:Phospholipases; D014466:Ultraviolet Rays
D007527:Isoenzymes; D008081:Liposomes; D008563:Membrane Lipids; D002784:Cholesterol; D010740:Phospholipases; D000110:Acetylcholinesterase
Vliianie sostoianiia lipidnoĭ fazymembrany na éffektivnost' fotokhimicheskoĭ modifikatsii éritrotsitarnoĭ atsetilkholinésterazy.