Isoelectric focusing in polyacrylamide gel carried out with a simple device for power regulation. Application to mammalian growth hormones.
A unit is described to regulate the electrical power delivered to an isoelectric focusing cell. The device consists in a resistance set, in series with a power supply, which can be adjusted to a suitable resistance value in order to permit only minor changes in wattage. Simultanoeus monitoring of voltage and current is also carried out with a pen-recorder adaptor. The improvements in operative conditions achieved by these means are tested by the fractionation of mammalian growth hormones.
D000818:Animals; D001001:Anura; D002415:Cats; D004591:Electrophoresis, Polyacrylamide Gel; D013006:Growth Hormone / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing / Q000295:instrumentation*; D051381:Rats; D012756:Sheep; D013552:Swine
D013006:Growth Hormone