Effect of human uterine flushings collected at various stages of the menstrual cycle on mouse blastocysts in vitro.
Mouse blastocysts were cultured in vitro in a defined medium supplemented with uterine flushings (containing 500 microgram protein/ml) obtained from normal women at various stages of the menstrual cycle. With one exception (uterine flushing collected on the last day of a menstrual period) blastocyst hatching and attachment were not impaired by flushings collected before or after ovulation.
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D001826:Body Fluids; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008598:Menstruation; D051379:Mice; D013997:Time Factors; D014599:Uterus / Q000502:physiology*