[Sensitivity and specifitivity of the counterimmunoelectrophoresis for determination of precipitating antibodies (author's transl)].
Counterimmunoelectrophoresis (C.I.E.) is suitable for routine-work in clinical medicine to give quantitative evidence of precipitating antibodies. Sensitivity of this method is high (lowest level being 200 to 400 ng albumin/ml serum) and range of evidence being very broad (14-15 titer steps). The direction of migrations of proteins with anodal migration (Ig) is changed by carbamylation. The experience gained in more than 3,000 investigations, concerning methods and evaluation of the results, is reported in detail. Counterimmunoelectrophoresis is superior to immunoelectrophoresis and double diffusion (Ouchterlony) due to its higher sensitivity and to RAST or RIST by reason of its lower costs.
D000941:Antigens; D003377:Countercurrent Distribution; D003485:Cyanates; D007122:Immunoelectrophoresis / Q000592:standards*; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D011234:Precipitins / Q000032:analysis*; D012709:Serum Albumin
D000941:Antigens; D003485:Cyanates; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011234:Precipitins; D012709:Serum Albumin
Sensibilität und Spezifität der Gegenstromelektrophorese beim Nachweis präzipitierender Antikörper.