A radioenzymatic method to measure picogram amounts of dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC) in small samples of brain tissue.
A radioenzymatic method for simultaneous determination of dopamine and DOPAC in small brain areas is described. By using this assay, 250 pg of dopamine and 150 pg of DOPAC can be estimated. The present method has been applied to compare the effect of different psychotropic drugs on the dopamine and DOPAC levels in the caudate nucleus, substantia nigra and medial basal hypothalamus.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002394:Catechol O-Methyltransferase; D002421:Caudate Nucleus / Q000032:analysis; D004298:Dopamine / Q000032:analysis*; D007031:Hypothalamus / Q000032:analysis; D008745:Methylation; D008832:Microchemistry / Q000379:methods; D010648:Phenylacetates / Q000032:analysis*; D051381:Rats; D013378:Substantia Nigra / Q000032:analysis
D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D002394:Catechol O-Methyltransferase; D004298:Dopamine