Studies on the uptake and metabolism of dibenz(b,f)-1,4-oxazepine (CR) by guinea-pig cornea.
The uptake and metabolic fate of the sensory irritant dibenz(b,f)-1,4-oxazepine (CR) have been investigated in intact cornea and corneal homogenates using 3H-labelled CR. The cornea metabolises CR to its lactam derivative (10,11-dihydro-10-oxo-dibenz(b,f)-1,4-oxazepine) by a process which appears to be located in the cell cytoplasm and which is easily saturated. Uptake studies with higher concentrations of [3H]CR (1 X10(-5) M) which saturate lactam biosynthesis, suggest that CR may bind, with low affinity, to sites within the corneal cells. The possible roles of CR metabolism and CR binding in chemical sensory irritation are discussed.
D000818:Animals; D003315:Cornea / Q000187:drug effects / Q000378:metabolism*; D003989:Dibenzoxazepines / Q000378:metabolism* / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008745:Methylation; D013997:Time Factors
D003989:Dibenzoxazepines