title: Integration of alkaline phosphatase in the intestinal brush border membrane.
abstract: Alkaline phosphatase has been solubilized from porcine intestinal mucosa by two different methods: treatment of the mucosa by Emulphogen BC 720 and papain hydrolysis of enterocyte brush border membrane vesicles. Two differnt enzyme forms have been obtained by these methods. The two enzyme forms ('detergent form' and 'papain form') have been purified to homogeneity by similar techniques and exhibit closely related molecular characteristics. However, the detergent form displays a hydrophobic behaviour and aggregates in media free of detergent. The two forms can be differentiated by their electrophoretic mobility on polyacrylamide gel in the absence of sodium dodecyl sulphate. By electrophoresis on polyacrylamide gel in the presence of sodium dodecyl sulphate, it has been shown that the detergent and papain forms of alkaline phophatase are dimers consisting of two apparently identical subunits whose molecular weights are 64 000 and 61 000, respectively. The difference between these molecular weights has been attributed to the existence of a hydrophobic region in the detergent form which is present on each subunit.
mesh_terms: D000469:Alkaline Phosphatase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology*; D003902:Detergents; D007082:Ileum / Q000201:enzymology; D007413:Intestinal Mucosa / Q000201:enzymology*; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007583:Jejunum / Q000201:enzymology; D008871:Microvilli / Q000201:enzymology*; D008970:Molecular Weight; D013552:Swine
chemical_list: D003902:Detergents; D007527:Isoenzymes; D000469:Alkaline Phosphatase