Isolation by molecular cloning of a fragment in the split ovalbumin gene.
An EcoRI fragment of chicken DNA (fragment 'a') containing a sequence complementary to the 3' half of ovalbumin mRNA has been isolated by molecular cloning. Analysis of the cloned fragment proves conclusively that the chicken ovalbumin gene is split. Fragment 'a' contains no extensive sequence repeated elsewhere in the genome and represents the only type of organisation of this part of the split ovalbumin gene in chicken genome.
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002874:Chromosome Mapping; D003090:Coliphages / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000302:isolation & purification*; D005796:Genes; D010047:Ovalbumin / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012333:RNA, Messenger; D010047:Ovalbumin; D004262:DNA Restriction Enzymes