title: [Solid-phase radioimmunoassay for class specific immunoglobulins against tick-borne encephalitis virus (author's transl)].
abstract: By adsorbing purified Tick-borne encephalitis (TBE) virus antigen onto beads of polystyrene, a solid-phase radioimmunoassay (RIA) for specific antibodies of the IgM- and IgG-class in untreated and nonfractionated human sera could be developed. The antibodies were measured by antihuman-gamma and antihuman-mu globulin labelled with 125 I. The RIA was about 20 times as sensitive as the hemagglutination-inhibition test. About 10% more cases of TBE could be diagnosed with the RIA than with the 2-mercaptoethanol test.
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D008590:Meningoencephalitis / Q000276:immunology*; D011137:Polystyrenes; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007075:Immunoglobulin M; D007136:Immunoglobulins; D011137:Polystyrenes
vernacular_title: Festphasen-Radioimmunoassay zur Messung klassenspezifischer Immunglobuline gegen das Virus der Frühsommer-Meningoenzephalitis.