Regulation of proliferation of murine megakaryocyte progenitor cells by cell cycle.
The extent to which mouse megakaryocyte progenitor cells (colony-forming unit-megakaryocyte, CFU-M) can proliferate in semisolid cultures prior to endomitosis, and conditions that may regulate that differentiation step, were investigated. The proliferative capacity of CFU-M was determined by estimating the number of megakaryocytes per colony. A bimodal distribution was observed (modal values, 10-15 and 25-30 cells/colony), indicating that separate megakaryocyte progenitor cells may be biased in their capacity for proliferation versus endomitosis. Differences were observed in the cell cycle characteristics of CFU-M as determined in vivo and in vitro that suggest that maturation of CFU-M into megakaryocytes may be regulated within the marrow by control of the cell cycle of the megakaryocyte precursor cell.
D000818:Animals; D001854:Bone Marrow Cells; D002453:Cell Cycle; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D006098:Granulocytes / Q000166:cytology; D006918:Hydroxyurea / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011828:Radiation Chimera
D003470:Culture Media; D006918:Hydroxyurea