A fractionation of insoluble rat skin collagen.
After extraction of 1% of the total collagen of rat skin by 150 mM NaCl, a 1% SDS solution extracts 80% of the total collagen, while the residue is extracted by a mixture of beta mercapto-ethanol and SDS. These extractions, which respect the peptide linkages, cleave the allysyl cross-links. They provide an insight on the composition of some insoluble collagen molecules of the skin and on the importance of disulfide bridges in their architecture.
D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003094:Collagen / Q000032:analysis*; D008623:Mercaptoethanol; D008968:Molecular Conformation; D051381:Rats; D012867:Skin / Q000032:analysis; D012967:Sodium Dodecyl Sulfate
D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol; D003094:Collagen