Interaction between cell sodium and the amiloride-sensitive sodium entry step in rabbit colon.
Ouabain abolishes the short-circuit current (Isc) and decreases the transepithelial conductance (Gt) of rabbit colon. In contrast, amphotericin B elicits a maximum Isc and markedly increases Gt. However, in both instances the amiloride-sensitive Na entry step is completely blocked, presumably due to an increase in cell Na. Conversely, when Na-depleted tissues are suddenly exposed to 140 mM Na, the amiloride-sensitive Isc and the amiloride-sensitive component  of Gt (alphaGNa) increase abruptly to their maximum values and then decline to steady-state plateaus with a half time of approximately 6 min; throughout the decline (Isc/alphaGNa) = ENa is constant at a value of 95 mV. In the presence of amphotericin B, the Isc abruptly rises to the same maximum but does not decline. These findings indicate that in the presence of 140 mM Na the conductance of the amiloride-sensitive Na entry step can vary from a maximum value of approximately 1.6 mmhos/cm2 when cell Na is depleted, to zero when cell Na is abnormally elevated (e.g., in the presence of ouabain or amphotericin B). Our findings are consistent with a system in which the pathway responsible for transcellular Na transport parallels another cellular compartment with which it communicates. The Na capacity of the active transport pathway appears to be very small so that this compartment fills rapidly after exposure of Na-depleted cells to 140 mM Na, and active transepithelial Na transport is initiated and reaches steady-state levels quickly. The Na capacity of the second compartment is much larger; the Na content of this compartment appears to be responsible for the negative feedback effect on the permeability of the amiloride-sensitive entry step.
D000584:Amiloride / Q000494:pharmacology*; D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003106:Colon / Q000378:metabolism*; D004553:Electric Conductivity; D005260:Female; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D011719:Pyrazines / Q000494:pharmacology*; D011817:Rabbits; D012964:Sodium / Q000378:metabolism* / Q000494:pharmacology
D011719:Pyrazines; D010042:Ouabain; D000584:Amiloride; D000666:Amphotericin B; D012964:Sodium