title: Clean-up procedure for the extraction of soil samples in the determination of 2,3,7,8-tetrachlorodibenzo-p-dioxin.
abstract: This paper describes the method which was developed in relation to analytical work connected with microbial and physico-chemical degradation experiments on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Soil samples are best extracted with methanol plus methylene chloride. Microbial preparations are extracted with light petroleum after boiling with methanolic potassium hydroxide. The clean-up consists of a sulphuric acid treatment and chromatography on a multilayer column (Celite + H2SO4/silica gel) followed by alumina column chromatography. The clean-up procedure proved to be suitable for soil samples and microbial preparations even when large quantities of organic matter (hydrocarbons, oils, surfactants) were present.
mesh_terms: D001711:Biotransformation; D004147:Dioxins / Q000032:analysis*; D007220:Industrial Waste / Q000032:analysis; D007558:Italy; D000072317:Polychlorinated Dibenzodioxins / Q000032:analysis*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D004147:Dioxins; D007220:Industrial Waste; D000072317:Polychlorinated Dibenzodioxins; D012989:Soil Pollutants