The detection of mutation in human diploid fibroblasts after mutagen treatment using non-selective cloning and enzyme electrophoresis.
In an attempt to study mutagenesis in human diploid fibroblasts, clones derived from mass cultures treated with mutagen have been examined by starch-gel electrophoresis for 43 different enzyme loci. A technique of mutagen treatment was devised which facilitated the cloning and which enabled the cells to be exposed to very high doses of EMS and MNNG. Two alterations in phenotype, presumably the result of mutation, were observed, one involving peptidase D (PEPD) and the other phosphoglucomutase (PGM1).
D002999:Clone Cells; D004171:Diploidy; D004592:Electrophoresis, Starch Gel; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens; D009154:Mutation
D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D005020:Ethyl Methanesulfonate