Undersulfated urinary glycosaminoglycans in preterm newborns.
The glycosaminoglycans (GAG) isolated from pooled urine of preterm newborns were fractionated by stepwise elution from a Dowex 1-X2 column. Analytical reactions, cellulose acetate electrophoresis and enzymatic digestions with chondroitinases and testicular hyaluronidase were performed on each fraction. Chondroitin-4-sulfate and chondroitin-6-sulfate constitute about 60% of urinary GAG; heparan sulfate amounts to about 20%, while chondroitin represents 12% of total GAG; dermatan sulfate, hyaluronic acid and keratan sulfate are present in small traces. Approximately 30% of the total is constituted by nonsulfated GAG.
D002810:Chondroitinases and Chondroitin Lyases / Q000494:pharmacology; D002845:Chromatography; D003238:Connective Tissue / Q000378:metabolism; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006025:Glycosaminoglycans / Q000652:urine*; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D007231:Infant, Newborn; D007234:Infant, Premature; D008297:Male
D006025:Glycosaminoglycans; D006821:Hyaluronoglucosaminidase; D002810:Chondroitinases and Chondroitin Lyases