Use of [75Se]selenomethionine in immunoglobulin biosynthetic studies.
The gamma-emitting amino acid analog, [75Se]selenomethionine, has been used as a biosynthetic label for immunoglobulins secreted by plasmacytomas in tissue culture. The secreted products are structurally intact with respect to their antibody combining sites and their class and allotype antigenic specificities. A component of [75Se]selenomethionine preparations was found to bind to fetal calf serum proteins, in a manner releasable by mercaptoethanol, but not by sodium dodecyl sulfate (SDS) and urea. Methods for circumventing the problems caused by this binding are described.
D000818:Animals; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D007136:Immunoglobulins / Q000096:biosynthesis*; D007164:Immunosorbents; D051379:Mice; D008807:Mice, Inbred BALB C; D008814:Mice, Inbred NZB; D009101:Multiple Myeloma / Q000276:immunology; D010767:Phosphorylcholine / Q000378:metabolism; D011863:Radioimmunoassay; D012643:Selenium / Q000378:metabolism*; D012645:Selenomethionine / Q000378:metabolism*
D003470:Culture Media; D007136:Immunoglobulins; D007164:Immunosorbents; D010767:Phosphorylcholine; D012645:Selenomethionine; D012643:Selenium