A radioimmunoassay method for urinary catechol estrogens.
A radioimmunoassay method for urinary catechol estrogens is described; The specific nature of the antisera allows direct analyses of acid hydrolyzed urine. A LH-20 Sephadex column chromatography can be employed for individual determinations of 2-hydroxyestrone and 2-hydroxyestradiol. The excretion of catechol estrogens during menstrual cycles ranged from 14.48 to 50.15 microgram per 24 hours, whereas, during the last trimester of pregnancies, the values ranged from 129.30 to 1758. 20 microgram per 24 hours.
D000918:Antibody Specificity; D001205:Ascorbic Acid / Q000494:pharmacology; D003429:Cross Reactions; D004967:Estrogens / Q000652:urine; D004970:Estrone / Q000031:analogs & derivatives*; D005260:Female; D006801:Humans; D006894:Hydroxyestrones / Q000276:immunology / Q000302:isolation & purification / Q000652:urine*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D011863:Radioimmunoassay / Q000379:methods; D013696:Temperature
D004967:Estrogens; D006894:Hydroxyestrones; D004970:Estrone; D001205:Ascorbic Acid