Interaction between Rhizobium japonicum phage M-1 and its receptor.
The receptor for phage M-1 was present in the exopolysaccharide (EPS) of Rhizobium japonicum D211. The EPS was a heteropolysaccharide consisting of glucose, galactose, glucuronic acid, and glucosamine units. These monosaccharides prevented phage-cell attachment indicating that they may mimick the receptor. Phage-cell attachment was either stimulated or inhibited by Mg2+ and Ca2+ depending upon their concentration. An enzyme which depolymerized the exopolysaccharide releasing oligosaccharides was detected in the phage-infected cell lysates. A comparison of the properties of adsorption and those of the depolymerase enzyme indicated that the latter was a component of the phage and appeared to be involved in the phage-receptor interaction.
D000327:Adsorption; D001435:Bacteriophages / Q000201:enzymology / Q000254:growth & development*; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D005965:Glucuronates / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000378:metabolism; D012231:Rhizobium
D005965:Glucuronates; D011135:Polysaccharides, Bacterial; D006026:Glycoside Hydrolases; D005947:Glucose; D005944:Glucosamine; D005690:Galactose