Organ culture studies on human skin and cholesteatoma epithelium. Contact with connective tissue and exposure to vitamin A.
In tissue culture trunk skin from 8-day-old chick embryos showed a consistent change from keratinizing into more columnar non-keratinizing epithelium under the influence of vitamin A acid using concentrations from 10 to 20 IU/ml. Human adult postauricular and ear canal skin, cholesteatoma membrane, and human embryonic skin retained their keratinizing properties when exposed to vitamin A acid at concentrations up to 150 IU/ml, when toxic changes in the epithelium became obvious. Treatment of cholesteatoma ears with vitamin A ear drops is unlikely to effect any change in the keratinizing properties of the epithelium.
D000818:Animals; D002642:Chick Embryo; D002781:Cholesteatoma; D003238:Connective Tissue / Q000187:drug effects; D003470:Culture Media; D046508:Culture Techniques; D004424:Ear Canal; D004848:Epithelium / Q000187:drug effects; D006801:Humans; D012867:Skin / Q000187:drug effects* / Q000196:embryology; D014212:Tretinoin / Q000494:pharmacology*; D014801:Vitamin A / Q000031:analogs & derivatives*
D003470:Culture Media; D014801:Vitamin A; D014212:Tretinoin