Improved iodination of peptides for radioimmunoassay and membrane radioreceptor assay.
Generally applicable methods for iodinating and purifying small peptide radiolabels for radioimmunoassay and membrane radioreceptor are described in detail. Resulting improvements in radioreceptor assay and radioimmunoassay, as well as results of analyses of specific activity, separation from unlabeled peptide, and storage characteristics, are presented for luliberin, corticotropin, melanotropin, and calcitonin.
D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D010455:Peptides / Q000138:chemical synthesis* / Q000302:isolation & purification; D011863:Radioimmunoassay; D011869:Radioligand Assay; D013997:Time Factors
D007457:Iodine Radioisotopes; D010455:Peptides