Enzymatic determination of triglycerides with a system Olli 3000 analyser.
We describe a totally enzymatic method for determining serum triglycerides adapted for a System Olli 3000 analyser. In this procedure triglycerides are determined by measuring free glycerol enzymatically after hydrolysis of a sample with lipase and esterase. The method utilizes two standards for calibration, includes a blank correction and requires 20 microliter of serum. Forty serum samples can be analysed in about 30 min. The procedure is linear up to a concentration of 8 mmol/l of triglycerides. The precision and sensitivity of the method are good. A comparison of this method with another enzymatic method gave the correlation coefficient 0.988 and the regression line y = 1.02x + 0.02 (n = 95).
D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods*; D005991:Glycerol Kinase; D006801:Humans; D014280:Triglycerides / Q000097:blood*
D014280:Triglycerides; D005991:Glycerol Kinase