[Protein and nucleic acid biosynthesis in tissue lymphocytes frozen at -196 degrees C with PEG-400].
DNA, RNA and protein synthesis was studied in splenocyte suspensions after superlow cooling (-196 degrees C) with PEG-400. It is shown that both standard two-step cooling and cooling with a holding temperature (at -28 degrees C) reduce precursors incorporation into DNA, proteins and particularly RNA. The splenogram analysis and supravital staining suggest that the observed changes are at least partially due to splenocytes death and the redistribution of cellular contents in the direction of the preferential survival of small lymphocytes. A lack of complete correlation between a reduction in biosynthesis of the macromolecules under study and cell viability testifies to the direct damage of proteinsynthetizing system components.
D000818:Animals; D002470:Cell Survival; D004247:DNA / Q000096:biosynthesis*; D005615:Freezing; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011092:Polyethylene Glycols / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D013154:Spleen / Q000166:cytology
D011092:Polyethylene Glycols; D012313:RNA; D004247:DNA
Biosintez nukleinovykh kislot i belkov v tkanevykh limfotsitakh, podvergnutykh zamorazhivaniiu DO -196 grandusov C pod zashchitoĭ polietilenglikolia-400.