title: Glutathione S-transferase in human lymphoid cell lines and fractionated peripheral leucocytes.
abstract: Glutathione S-transferase activity was identified in cytosol from human lymphoid-cell lines and peripheral leucocytes (polymorphonuclear-leucocyte/monocyte and small-lymphocyte fractions) and compared with human liver enzyme. The findings of closely similar elution volume in gel filtration, substrate (1-chloro-2,4-dinitrobenzene) and inhibitory (probenecid) kinetics indicate that the liver, leucocyte and lymphoid-cell transferases are closely related. The interaction of reduced glutathione and 1-chloro-2,4-dinitrobenzene was shown to occur in intact-lymphoid-cell culture, to be linear with time and quantity of cells and to have kinetics similar to those of the enzyme reaction catalysed by cytosol.
mesh_terms: D002460:Cell Line; D002850:Chromatography, Gel; D003600:Cytosol / Q000201:enzymology; D004137:Dinitrochlorobenzene / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology*; D011339:Probenecid / Q000494:pharmacology
chemical_list: D004137:Dinitrochlorobenzene; D005982:Glutathione Transferase; D005978:Glutathione; D011339:Probenecid