Studies on bovine insulin with methyl vinyl ketone. Isolation of crystalline A1-3-oxobutyl derivative of bovine insulin.
Reaction of methyl vinyl ketone with bovine insulin affords, amongst others, a crystalline A1-(3-oxobutyl)insulin. This retains full biological activity in the mouse convulsion assay. The presence of zinc ions changes the course of the above reaction, and illustrates the use of zinc as a "blocking function".
D000818:Animals; D002417:Cattle; D003460:Crystallization; D007202:Indicators and Reagents; D007328:Insulin / Q000031:analogs & derivatives / Q000302:isolation & purification*; D007659:Ketones; D051379:Mice; D015032:Zinc
D007202:Indicators and Reagents; D007328:Insulin; D007659:Ketones; D015032:Zinc