title: Cytochemistry of marrow and extramedullary adipocytes in monolayer cultures.
abstract: The behavior of isolated, disaggregated white adipose cells obtained from marrow and extramedullar sites in the rabbit was compared in monolayer cultures. Both cell types transform within 2 weeks into fibroblast-like cells which can be maintained in subcultures. Both in freshly isolated state and in fibroblast-like state the marrow adipocyte contains esterase activities reacting with naphthol AS acetate and D-chloracetate. These activites are not present in extramedullary cells. In addition, alpha-naphthyl butyrate-reacting esterase, present in both cell types, is fluoride-resistant in marrow adipocytes but labile in extramedullar cells. These differences are consistent with heterogeneity of white adipose tissue and suggest different potentials for marrow and extramedullary adipocytes.
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D001853:Bone Marrow / Q000201:enzymology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004950:Esterases / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology; D006651:Histocytochemistry; D011817:Rabbits
chemical_list: D004950:Esterases