A simple apparatus for performing short-time (1--2 seconds) uptake measurements in small volumes; its application to D-glucose transport studies in brush border vesicles from rabbit jejunum and ileum.
An automated procedure allows uptake measurements with incubation times as short as 0.5 s and with volumes of 10--20 microliter. Using this technique the kinetic parameters Km and V of D-glucose transport in brush border vesicles from rabbit small intestine could be determined from unidirectional fluxes. A comparison of the data obtained from jejunum and from ileum shows that the Km for D-glucose is the same in both parts of the intestine, whereas the maximum flux is significantly larger in the jejunum.
D000818:Animals; D001692:Biological Transport; D002474:Cell-Free System; D005374:Filtration; D005947:Glucose / Q000378:metabolism*; D007082:Ileum / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism*; D007700:Kinetics; D008722:Methods; D008871:Microvilli / Q000378:metabolism; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology
D012964:Sodium; D005947:Glucose