A new micromethod for colorimetric determination of zinc in serum.
A simple micromethod with a colorimetric reagent of high sensitivity was developed for the determination of zinc in serum. For the color reagent 2-(2-pyridylazo)-5-dimethylaminophenol (PADAP) was used. Coefficients of variation of the within-day precision were 6.65 and 2.24% for zinc standard at concentrations of 0.1 and 0.5 microgram/ml, respectively. The mean recovery by this method was 101.2% (S.D.: 3.56%). Zinc concentrations measured by the above method showed good agreement with those by atomic absorption spectrophotometry, with a correlation coefficient of 0.934. Serum zinc values of 50 healthy adults (38 females, 12 males) ranged from 63 to 116 microgram/100 ml (mean: 89.68 microgram/100 ml, Sd.: 13.28 microgram/100 ml).
D000293:Adolescent; D000328:Adult; D001391:Azo Compounds; D005260:Female; D006801:Humans; D008027:Light; D008297:Male; D008722:Methods; D008832:Microchemistry; D008875:Middle Aged; D012016:Reference Values; D013053:Spectrophotometry; D013997:Time Factors; D015032:Zinc / Q000097:blood*
D001391:Azo Compounds; D015032:Zinc