Relative rates of tumor, normal gut, liver, and fibrinogen protein synthesis in man.
Fractional protein synthesis rates of tumor, normal intestinal tissue, liver, and fibrinogen were measured in a series of patients with gastrointestinal malignancies. Protein synthesis rates were measured by the continuous infusion of 95+% [15N]glycine. Twelve to eighteen hours prior to the clinically indicated surgical excision of the tumor, 1-2 g of 95+% [15N]glycine was infused at a constant rate into each subject. During the surgical procedure, 0.05-2 g of tumor, normal intestinal tissue, liver, and 30 ml of venous blood were obtained. Protein synthesis rates were estimated from the ratio of 15N incorporated into tissue protein to the 15N enrichment of the tissue-free amino acid pool. The major findings were: i) the 15N enrichment of the tissue-free amino acids in malignant tissue was greater than and proportional to that in the corresponding normal tissue (P less than 0.02); ii) tumor protein synthesis rates were greater and proportional to the corresponding intestinal tissue rates (P less than 0.05); iii) the fibrinogen synthesis rate was greater than the liver protein synthesis rate (P less than 0.01), but there was no correlation between them.
D000328:Adult; D005340:Fibrinogen / Q000096:biosynthesis*; D005770:Gastrointestinal Neoplasms / Q000097:blood / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006801:Humans; D007263:Infusions, Parenteral; D007413:Intestinal Mucosa / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis
D009363:Neoplasm Proteins; D005340:Fibrinogen; D005998:Glycine