Electrophoretic identification of the brain isoenzyme of creatine kinase following treatment with anti-BB antisera.
A procedure is described for accurately determining the presence of true creatine kinase isoenzyme BB by fluorescent staining following electrophoresis on either cellulose acetate or agarose. Treatment of sera with anti-BB inhibiting antibody prior to electrophoresis and subsequent enzymatic staining allows clear identification of creatine kinase BB in presence of interfering artifacts. The utilization of an immunological means of identifying BB following electrophoresis avoids the pitfall of associating an artifactual BB isoenzyme with a disease state. Using this technique, presence of creatine kinase BB in patients with carcinoma, cardiac disease and renal failure has been verified.
D000906:Antibodies; D001667:Binding, Competitive; D001921:Brain / Q000201:enzymology*; D003402:Creatine Kinase / Q000276:immunology / Q000378:metabolism*; D004588:Electrophoresis, Cellulose Acetate; D005453:Fluorescence; D006801:Humans; D007106:Immune Sera; D007527:Isoenzymes / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D007106:Immune Sera; D007527:Isoenzymes; D003402:Creatine Kinase