Differential frequency of human X and Y chromatin as related to cell density in vitro.
The relationship between cell density in vitro and the frequency of fluorescent X chromatin and/or Y chromatin-positive interphase nuclei was studied in cultured cells from three human male and female subjects. It was found that altough the frequency of fluorescent X chromatin-positive cells was proportional to cell density, the frequency of fluorescent Y chromatin-postitive cells was independent of cell density. Our results are compared with those of other investigators.
D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D005453:Fluorescence; D006801:Humans; D008297:Male; D012728:Sex Chromatin / Q000648:ultrastructure*; D012730:Sex Chromosomes / Q000648:ultrastructure*; D014960:X Chromosome / Q000648:ultrastructure*; D014998:Y Chromosome / Q000648:ultrastructure*