Affinity chromatography of phenylalanine hydroxylase. The structure of a pteridine adsorbent.
1. Four independent methods have established that the structure of a previously reported pteridine affinity adsorbent, 6,7-dimethyl-5,6,7,8-tetrahydropterin--CH-Sepharose, is 5(CH-Sepharosyl)-6,7-dimethyl-5,6,7,8-tetrahydropterin. 2. A novel reaction, the carbodiimide-promoted coupling of a carboxyl group to N-5 of a tetrahydropterin, is described. 3. Two novel adsorbents, 5-formyl-tetrahydrofolate--AH-Sepharose and 5-methyl-tetrahydrofolate--AH-Sepharose, are described which may be useful not only in the study of phenylalanine hydroxylase but also in the study of folate-metabolizing enzymes.
D000327:Adsorption; D002846:Chromatography, Affinity / Q000379:methods; D010651:Phenylalanine Hydroxylase / Q000302:isolation & purification*; D011621:Pteridines
D011621:Pteridines; D010651:Phenylalanine Hydroxylase