Satellite DNA (II) from sea urchin (Lytechinus variegatus) sperm.
When DNA isolated from freshly collected sperm of sea urchin (Lytechinus variegatus) is centrifuged to equilibrium in CsCl, 2 heavy satellite bands appear beside the main band DNA. Satellite DNA (II) appears in between the main band DNA (rho = 1.695 g/cm3) and the rDNA satellite (rho = 1.722 g/cm3). Satellite DNA (II) has a buoyant density 1.710 g/cm3, corresponding to 50% GC content. It is speculated that the satellite DNA (II), which appears to be of high mol. wt, might contain the sequences complementary to histone mRNA.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000302:isolation & purification*; D004276:DNA, Satellite / Q000302:isolation & purification*; D003720:Densitometry; D066298:In Vitro Techniques; D008297:Male; D012617:Sea Urchins / Q000032:analysis / Q000166:cytology*; D013094:Spermatozoa / Q000032:analysis*
D004276:DNA, Satellite; D004247:DNA