[Physico-chemical study of the nucleic acid of bacteriophage phiB].
A study of physico-chemical properties of the nucleic acid of phage Brevibacterium flavum was made. It was found that the phage nucleic acid is a double-stranded DNA. The content of GC pairs was determined by the temperature of DNA melting and by the chromatographic method. In both cases it was (52 +/- 2) mole %. It has been shown that endonuclease Eco RI treatment of phiB DNA forms 12 fragments. The molecular weight of phiB DNA has been determined by two independent methods: by measuring the contous length of DNA and by the sum of molecular weights of DNA restricts obtained after hydrolysis by endonuclease Eco RI and was found to be (30 +/- 1) . 10(6) dalton. Electron microscopy investigations detected rod and circular DNA in either monomeric or dimeric forms. The polarity of DNA arrangement in the phiB phage particle was determined by using the partial denaturation maps.
D001435:Bacteriophages / Q000032:analysis*; D001951:Brevibacterium; D055598:Chemical Phenomena; D002621:Chemistry; D002627:Chemistry, Physical; D004262:DNA Restriction Enzymes; D004270:DNA, Circular; D004279:DNA, Viral / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D008854:Microscopy, Electron; D008970:Molecular Weight; D013696:Temperature
D004270:DNA, Circular; D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Fiziko-khlimicheskoe izuchenie nukleinovoĭ kisloty bakteriofaga phiB.