Blood preservation XXVII. Fructose and mannose maintain ATP and 2,3-DPG.
Mannose and fructose as well as glucose have been shown to be effective for maintaining ATP and thus viability of stored red blood cells. Normal 2,3-DPG levels are desirable in stored red blood cells to provide the needed oxygen transport upon transfusion. ATP levels in sotred concentrated red blood cells in the new preservative, CPD-adenine (citrate-phosphate-dextrose-adenine) become critically low in the 5th week. In this study two hexoses and two pentoses are compared with dextrose in their ability to maintain ATP and 2,3-DPG. ATP levels were best maintained by fructose, then dextrose and mannose. ATP levels had fallen to critically low levels by four weeks with ribose and xylose. Red blood cell 2,3-DPG concentrations were also maintained by hexoses, with mannose being best, dextrose and fructose being similar. When ribose was used in addition to dextrose in CPD-adenine, ATP maintenance was improved and under the same conditions xylose improved 2,3-DPG maintenance. Fructose and mannose may be as useful as dextrose in citrate-phosphate preservatives for maintaining ATP and 2,3-DPG levels. Also, ribose and xylose may help the maintenance of ATP and 2,3-DPG, respectively, in CPD-adenine.
D000136:Acid-Base Equilibrium; D000255:Adenosine Triphosphate / Q000097:blood*; D001793:Blood Preservation; D002951:Citrates / Q000032:analysis; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis; D005632:Fructose / Q000494:pharmacology*; D005947:Glucose / Q000032:analysis; D005958:Glucosephosphates / Q000032:analysis; D006601:Hexoses / Q000032:analysis*; D008358:Mannose / Q000494:pharmacology*; D010429:Pentoses / Q000032:analysis*; D010865:Pilot Projects; D012266:Ribose / Q000032:analysis; D014994:Xylose / Q000032:analysis
D002951:Citrates; D004163:Diphosphoglyceric Acids; D005958:Glucosephosphates; D006601:Hexoses; D010429:Pentoses; D005632:Fructose; D012266:Ribose; D000255:Adenosine Triphosphate; D014994:Xylose; D005947:Glucose; D008358:Mannose