Determination of urinary indolic metabolites.
A rapid mass spectral assay for tryptophol, 5-hydroxytryptophol, and 3-indoleacetic acid, employing stable-isotope labeled internal standards, is described. The compounds were extracted from urine or buffer with ethyl acetate and quantitatively measured by chemical-ionization mass spectrometry. The calibration curves were linear over a range of 0.06--2.8 microgram/ml. The technique was applied to the analysis of urine from patients with carcinoid tumors. In addition, the first synthesis of 5-methoxy-3-indoleacrylic acid is reported.
D002276:Carcinoid Tumor / Q000652:urine*; D006801:Humans; D007210:Indoleacetic Acids / Q000652:urine; D007211:Indoles / Q000652:urine*; D013058:Mass Spectrometry; D014364:Tryptophan / Q000031:analogs & derivatives / Q000652:urine
D007210:Indoleacetic Acids; D007211:Indoles; D014364:Tryptophan