title: Sphincteric mechanism of the main pancreatic duct in the dog. An experimental model of the isolated sphincteric preparation.
abstract: This study describes an in vitro experimental model of the sphincter at the lower end of the main pancreatic duct in the dog. This model, employing a drop counter to measure the drop rate, monitors the perfusion rate of Tyrode through the sphincter. Acetylcholine (ACh), employed to establish the sensitivity and viability of the isolated sphincter, produced a contraction of the sphincter which was concentration-response related. Saline, as a control, atropine and hexamethonium did not affect the drop rate. Atropine (20 microgram) partially abolished the contraction produced by 50 microgram of ACh; hexamethonium (50 microgram) did not have an effect. Caerulein 50 microgram produced a relaxation of the sphincter; adrenaline 50 microgram a contraction. Both sympathetic and parasympathetic mechanisms seem to play a role in the regulation of sphincteric activity of the main pancreatic duct in dogs.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D014670:Ampulla of Vater / Q000502:physiology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004285:Dogs; D006584:Hexamethonium Compounds / Q000494:pharmacology; D008954:Models, Biological; D009119:Muscle Contraction / Q000187:drug effects; D010183:Pancreatic Ducts / Q000502:physiology*; D010477:Perfusion; D012965:Sodium Chloride / Q000494:pharmacology; D009803:Sphincter of Oddi / Q000502:physiology*
chemical_list: D006584:Hexamethonium Compounds; D012965:Sodium Chloride; D001285:Atropine; D000109:Acetylcholine