title: A simple and inexpensive culture device for cell growth, quantitative microscopy and autoradiography.
abstract: An inexpensive culture chamber suitable for subsequent preparations for cytochemical or radiographic work is demonstrated. The chamber consists of two glass microscope slides suitably prepared for cell growth and a gasket of silicone rubber. The slides and gasket are sealed and held together with two ordinary spring letter clips or clothes-pins. Cells may be inoculated by piercing the gasket with a hypodermic needle, or by removing the top slide and adding cells directly. Long-term cultivation with medium replacement is easy and efficient and addition of isotopes or other materials may be done aseptically.
mesh_terms: D001345:Autoradiography; D046508:Culture Techniques / Q000295:instrumentation*; D008853:Microscopy