title: Evidence for an endogenous factor interfering with 3H-diazepam binding to rat brain membranes.
abstract: An in vitro "receptor" binding assay has been used to search for an endogenous compound which possibly interacts with benzodiazepine receptors in brain. Such an endogenous 3H-diazepam binding inhibitory factor (DIF) has been found. This compound is unevenly distributed in brain and in various peripheral organs. The partially purified compound appears to have a low molecular weight (below 500) and is not inactivated by proteolytic enzymes.
mesh_terms: D000818:Animals; D001688:Biological Products / Q000494:pharmacology / Q000502:physiology*; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D003975:Diazepam / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008566:Membranes / Q000378:metabolism; D051379:Mice; D009928:Organ Specificity; D051381:Rats; D014020:Tissue Extracts / Q000032:analysis / Q000494:pharmacology*
chemical_list: D001688:Biological Products; D014020:Tissue Extracts; D003975:Diazepam