title: Factors in the movement of hexose across the intestine of Ascaris suum.
abstract: Trichloroacetic acid extractable carbohydrate of the intestine of Ascaris suum decreases rapidly when ribbons of the tissue are incubated in a basal salt solution. After 10 min incubation endogenous carbohydrate is 32% and after 80 min it is 19% of the "zero" time control value. In contrast, there is approximately a 2-fold increase in the endogenous carbohydrate of tissue that is incubated in saline with glucose. The increase occurs within the first 5 min and is maintained throughout an 80-min incubation period. Sac preparations of the intestine that are preincubated with glucose and incubated without glucose move 3-0-methylglucose from the luminal to the pseudocoelomic fluid at a rate that is comparable initially to the rate of movement measured for sac preparations that are incubated in saline with glucose. After 10 min the rate decreases. The addition of glycogen or trehalose to the saline bathing the pseudocoelomic side of sac preparations does not facilitated the movement of 3-0-methylglucose. Collectively, the results support the suggestion that the limited movement of  3-0-methylglucose across intestinal sac preparations that are incubated without glucose is due to the tissue's limited carbohydrate reserve and its rapid depletion in vitro.
mesh_terms: D000818:Animals; D001200:Ascaris / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000494:pharmacology; D006601:Hexoses / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D008757:Methylglucosides / Q000378:metabolism; D014199:Trehalose / Q000494:pharmacology
chemical_list: D006601:Hexoses; D008757:Methylglucosides; D006003:Glycogen; D014199:Trehalose; D005947:Glucose