Liquid chromatographic determination of daunorubicin and daunorubicinol in plasma from leukemic patients.
A method is given for the determination of daunorubicin and its main metabolite, daunorubicinol, in plasma from leukemic patients after administration of daunorubicin as the free drug or as a complex with DNA. Daunorubicin and daunorubicinol are extracted from 2 ml of plasma (pH 8.1) using a mixture of chloroform and 1-heptanol (9:1). After re-extraction into phosphoric acid (0.1 M), the separation is performed as reversed phase liquid chromatography on a LiChrosorb RP-2 (5 micrometer) column with a mobile phase of acetonitrile-water, acidified with phosphoric acid. The precision, by quantitation with a photometric detector, was better than 2% within the range 20 ng/ml to 200 ng/ml. Some determinations of plasma levels of daunorubicin and daunorubicinol are presented.
D002849:Chromatography, Gas / Q000379:methods; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000097:blood* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D007938:Leukemia / Q000097:blood* / Q000188:drug therapy; D012997:Solvents
D012997:Solvents; C000847:daunorubicinol; D003630:Daunorubicin