title: The adhesive interaction between polymorphonuclear leukocytes and endothelial cells in vitro.
abstract: The results of this investigation indicate an adhesive specificity between PMN and cultured endothelial cells. This was monitored by the mono-layer collection assay and by direct cell counts using the scanning electron microscope. Both techniques showed that significantly more PMN attached to endothelial cells than to a variety of other cell types. The interaction can be modulated by divalent cations and neuraminidase, implying a role for surface charge. In the presence of chemotactic agents, the number of PMN adhering to endothelial cells increases. This system presents a good model for studying the process of PMN margination which occurs in vivo during the acute inflammatory response.
mesh_terms: D001769:Blood; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002634:Chemotaxis, Leukocyte; D004727:Endothelium / Q000166:cytology*; D008274:Magnesium / Q000494:pharmacology; D009439:Neuraminidase / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology*; D010455:Peptides / Q000494:pharmacology
chemical_list: D010455:Peptides; D009439:Neuraminidase; D008274:Magnesium; D002118:Calcium