Quantitative determination of 3-ethyl-5-hydroxy-4,5-dimethyl-delta3-pyrrolin-2-one in urine using gas-liquid chromatography.
A method is presented for the quantitation in urine of 3-ethyl-5-hydroxy-4,5-dimethyl-delta3-pyrrolin-2-one, a substance associated with hepatic porphyria and psychiatric disorders. The method involves gas-liquid chromatography following solvent extraction using one of two other heterocyclic compounds as internal standards. Quantitation of this substance in the urine of a group of normal subjects is also reported.
D002849:Chromatography, Gas; D003404:Creatinine / Q000652:urine; D006801:Humans; D007536:Isomerism; D008722:Methods; D011758:Pyrroles / Q000652:urine*
D011758:Pyrroles; D003404:Creatinine