Estimation of correlation of lactate dehydrogenase subunits mole quota based on differences in substrate inhibition.
A kinetic method of estimating the mole quota ratios of the human lactate dehydrogenase (LDH) H and M subunits based on differences in substrate inhibition of LDH isoenzymes by lactate is porposed. Stability of kinetic constants for a prolonged period of time is demonstrated. The dependence of the activity ratios on the contribution of the mole quota of the M-subunit of LDH is studied under conditions of low and high substrate concentrations. The experimental and theoretical values show the following correlation: r = 0.998; p less than 0.001. A comparison of the method proposed with the electrophoretic method of LDH subunit estimation is made, the values obtained being in good agreement. No effect of the components of human diploid cell homogenate and only an insignificant effect of the blood serum components on the kinetic constants of LDH isoenzymes are shown. The applicability of the method to the estimation of the quantitative content of both LDH subunits in natural samples is demonstrated. The informational value of the method is compared to that of other standard methods of LDH isoenzyme estimation.
D004586:Electrophoresis; D006801:Humans; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000097:blood; D007773:Lactates / Q000494:pharmacology; D008099:Liver / Q000201:enzymology; D046911:Macromolecular Substances; D009206:Myocardium / Q000201:enzymology
D007527:Isoenzymes; D007773:Lactates; D046911:Macromolecular Substances; D007770:L-Lactate Dehydrogenase