Quantitative determination of tiflorex in human fluids using electron-capture detection.
A procedure is described for the determination of tiflorex and its metabolite nortiflorex in biological specimens. The compounds are converted into their trichoroacetyl derivatives, which are separated on a glass column packed with 3% OV-17 on Gas-Chrom Q, and measured with an electron-capture detector. The mechanism was investigated by gas chromatography-mass spectrometry. The method is rapid, sensitive for concentrations of 1 ng/ml and has been used to measure tiflorex and its metabolite in rat plasma after intravenous administration and in human volunteers after administration by the oral route.
D000818:Animals; D001067:Appetite Depressants / Q000008:administration & dosage / Q000097:blood*; D002849:Chromatography, Gas; D005277:Fenfluramine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D051381:Rats
D001067:Appetite Depressants; D005277:Fenfluramine; C016624:nortiflorex