Progesterone receptor in the rat anterior pituitary: effect of estrogen priming and adrenalectomy.
The present study was done to determine if a progesterone receptor is present in rat pituitary. Cytosol was labeled with 3H-progesterone (3HP) or 3H-R5020 (3HR) and subjected to sucrose-glycerol density-gradient centrifugation. Serum progesterone was measured for correlation with progesterone receptor levels. Two 3HP-binding peaks (4S + 6S) were evident in uterine and pituitary cytosols. The 4S peak was eliminated by competition with unlabeled cortisol leaving a single 6S peak (progesterone receptor). Estradiol (E) priming of the male or female rat increased progesterone receptor levels in pituitary cytosol as demonstrated using 3HP and 3HR, and pituitary progesterone receptor bound 3HR with a higher affinity than 3HP. Following adrenalectomy of gonadectomized rats, progesterone receptor levels were increased in pituitary and uterine cytosol of both E-primed and unprimed groups. An inverse relationship was established between serum progesterone and progesterone receptor levels in the uterus and pituitary suggesting that stress-induced adrenal progesterone secretion significantly influences progesterone receptor levels in the rat. These results demonstrate an estrogen-inducible progesterone receptor in the rat pituitary with properties similar to those of the uterine progesterone receptor.
D000315:Adrenalectomy; D000818:Animals; D002369:Castration; D003600:Cytosol / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D008297:Male; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011374:Progesterone / Q000097:blood; D045167:Progesterone Congeners / Q000378:metabolism; D051381:Rats; D011980:Receptors, Progesterone / Q000187:drug effects / Q000378:metabolism*
D045167:Progesterone Congeners; D011980:Receptors, Progesterone; D011374:Progesterone; D004958:Estradiol