Xeroderma pigmentosum fibroblasts of the D group lack an apurinic DNA endonuclease species with a low apparent Km.
Apurinic DNA endonuclease activity from cultured human fibroblasts was resolved into two species by phosphocellulose chromatography. The species had sedimentation coefficients of 3.3 S and 2.8 S and apparent Km's for apurinic sites of 5 and 44 nM, respectively. The low Km species was absent from extracts of cell lines XP5BE, XP6BE and XP7BE of xeroderma pigmentosum complementation group D.
D001080:Apurinic Acid; D002460:Cell Line; D003851:Deoxyribonucleases / Q000172:deficiency* / Q000302:isolation & purification; D004720:Endonucleases / Q000172:deficiency* / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D013379:Substrate Specificity; D014983:Xeroderma Pigmentosum / Q000201:enzymology* / Q000235:genetics
D001080:Apurinic Acid; D003851:Deoxyribonucleases; D004720:Endonucleases