A new storage medium for canine blood.
A solution consisting of ascorbate phosphate, citric acid, sodium citrate, sodium phosphate, and dextrose was developed to extend the shelf life of canine blood stored for transfusion. The 24-hour poststorage viability remained above 70% for 6 weeks of storage at 4 C. The concentration of 2,3 diphosphoglycerate remained constant for 3 weeks, then declined slowly. After 6 weeks of storage, the 2,3 diphosphoglycerate content was still sufficiently high to allow adequate dissociation of oxygen from oxyhemoglobin in vivo. It was concluded that blood stored up to 6 weeks in this solution would be safe to use for transfusion.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001793:Blood Preservation / Q000379:methods / Q000662:veterinary*; D001804:Blood Transfusion, Autologous / Q000662:veterinary; D004285:Dogs / Q000097:blood*; D004905:Erythrocyte Aging; D005260:Female; D008297:Male; D010710:Phosphates / Q000494:pharmacology; D013997:Time Factors
D010710:Phosphates; D001205:Ascorbic Acid