Incorporation of labelled glucose and glycerol into phospholipids and triglycerides by rat and rabbit aorta.
[1-14C]Glycerol and [U1-14C]glucose were incorporated into aortic phospholipids and triglycerides by isolated rat and rabbit aorta. The major portion of radioactivity was found in the glycerol moiety of triglycerides and phospholipids. Within the range of concentrations studied the rate of incorporation of [1-14C]glycerol was dependent on substrate concentration, whereas the rate of incorporation of [U1-14C]glucose was not. The distribution of radioactivity in the glycerophosphatides was found mainly in phosphatidylserine and phosphatidic acid when [1-14C]glycerol was used as substrate. On the other hand, the major portion of radioactivity was found in phosphatidylcholine when [U1-14C]glucose was the substrate. The incorporation of [1-14C]glycerol and [U1-14C]glucose into aortic triglycerides and phospholipids was markedly increased by aortae obtained from rabbits fed an atherogenic diet as compared to aortae from control animals. Results from double-labeled glycerol containing both [2-3H]glycerol and [1-14C]glycerol indicated that the biosynthesis of triglycerides and phospholipids in the rat aorta did not use phosphatidic acid from the same pool.
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism*; D001161:Arteriosclerosis / Q000209:etiology / Q000378:metabolism; D002250:Carbon Radioisotopes; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D007553:Isotope Labeling; D008297:Male; D010743:Phospholipids / Q000096:biosynthesis*; D011817:Rabbits; D051381:Rats; D014280:Triglycerides / Q000096:biosynthesis*
D002250:Carbon Radioisotopes; D010743:Phospholipids; D014280:Triglycerides; D005947:Glucose; D005990:Glycerol