Affinity chromatography of lactate dehydrogenase and wheat germ lectin on new gels bearing carboxylic functions.
The suitability of two new functionalized copolymer gels for use in affinity chromatography has been examined. Both gels were substituted by two ligands, one being specific for lactate dehydrogenase and the other for wheat germ lectin. The derivatives thus obtained were used successfully for the purification of two proteins with different biological activities.
D000818:Animals; D002846:Chromatography, Affinity; D005782:Gels; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D037102:Lectins / Q000032:analysis*; D009132:Muscles / Q000201:enzymology; D037121:Plant Lectins; D011817:Rabbits; D014908:Triticum
D005782:Gels; D037102:Lectins; D037121:Plant Lectins; D007770:L-Lactate Dehydrogenase