[Study of the chemoreceptor membrane of identified neurons of the gastropod mollusk Planorbarius corneus with different technics of applying chemicals].
Changes in the membrane potential caused by acetylcholine or gamma-aminobutyric acid when added to the perfusion fluid were compared with those induced by their local application to the neuron soma membrane from the micropipette (diameter 100-300 mu). With equal drug concentrations, the responses were found to be of similar amplitude with either method of application. The rate of changes in the membrane potential proved to be however several times higher in the case of local application. Using voltage--clamp technique and local drug application, the dose-response relationships were studied during selective activation either of two kinds of cholinoreceptors available on the identified neuron membrane. The saturation of the muscarinic receptors was shown to be attained at smaller agonist concentrations, the absolute value of maximal response being at least one order of magnitude lower than in case of nicotinic cholinoreceptors of the same cell.
D000109:Acetylcholine / Q000008:administration & dosage / Q000494:pharmacology*; D000613:Aminobutyrates / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D008974:Mollusca; D009474:Neurons / Q000187:drug effects; D011950:Receptors, Cholinergic / Q000187:drug effects*; D011976:Receptors, Muscarinic / Q000187:drug effects*; D011978:Receptors, Nicotinic / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000008:administration & dosage / Q000494:pharmacology*
D000613:Aminobutyrates; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D005680:gamma-Aminobutyric Acid; D000109:Acetylcholine
Issledovanie khemoretseptivnoĭ membrany identifitsirovannykh neĭronov briukhonogogo molliuska Planorbarius corneus pri raznykh sposobakh applikatsii veshchestv.