[Study of the toxic factor in liver extracts].
Dissolution of mitochondrial fraction of ischemic liver was carried out using various detergents, the most effective of which was sodium dodecylsulphate (SDS). Excess of SDS was removed by dialysis or by sorption on anion exchange resin. The optically homogenous solution obtained was subsequently purified by gel filtration on Sephadex G-75. The preparation contained two fractions: the molecular weight of the major fraction corresponded to that of gamma-globulin and the molecular weight of the minor fraction--to that of cytochrome C. Interrelation was observed between the toxic activity and hydrophobic components of the fraction isolated.
D000818:Animals; D004285:Dogs; D007511:Ischemia; D008099:Liver / Q000098:blood supply; D008110:Liver Extracts / Q000633:toxicity*; D008930:Mitochondria, Liver / Q000032:analysis
D008110:Liver Extracts
Izuchenie toksicheskogo faktora pechenochnykh ékstraktov.