[Accumulation of 14C-nicotinate by membrane preparations].
The incorporation of nicotinic acid (NA) into cells and mitochondria depends on temperature and the presence of glucose, is coupled with the activity of Na, K-ATPase and is disturbed by the energy exchange inhibitors. The addition of a sucrose liver extract or protein-containing fractions of the extract stimulates the 14C-NA accumulation by liver mitochondria. The stimulating effect of the extract on 14C-NA incorporation into mitochondria is retained after dialysis, but is removed upon boiling of the extract. It is assumed that the 14C-NA transport is operated by proteins: NA is incorporated (at least, partially) into liver cells, where it exists both in a free and proteinbound states. It has been found that there exist at least two protein fractions which bind 14C-NA.
D000818:Animals; D001692:Biological Transport; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism; D009539:Nicotinic Acids / Q000378:metabolism*
D002250:Carbon Radioisotopes; D009539:Nicotinic Acids
Akkumuliatsiia 14-C-nikotinata membrannymi preparatami.