title: Sinefungin, a potent inhibitor of virion mRNA(guanine-7-)-methyltransferase, mRNA(nucleoside-2'-)-methyltransferase, and viral multiplication.
abstract: Sinefungin (A9145) and a related metabolite, A9145C, were found to be potent inhibitors of Newcastle disease virion and vaccinia virion mRNA(guanine-7-)-methyltransferase and vaccinia virion mRNA(nucleoside-2'-)-methyltransferase. Both Sinefungin and A9145C were competitive inhibitors of these S-adenosyl-L-methionine-dependent enzymes having inhibition constants substantially less than S-adenosyl-L-homocysteine. These compounds also inhibited plaque formation by vaccinia virus in mouse L-cells.
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D009522:Newcastle disease virus / Q000187:drug effects / Q000201:enzymology; D013329:Structure-Activity Relationship; D014616:Vaccinia virus / Q000187:drug effects / Q000201:enzymology; D014779:Virus Replication / Q000187:drug effects*; D012359:tRNA Methyltransferases / Q000037:antagonists & inhibitors*
chemical_list: D000935:Antifungal Agents; D000998:Antiviral Agents; C016918:A9145C; D012359:tRNA Methyltransferases; D000241:Adenosine