Syncytia inhibition by immune lymphocytes: in vitro test for immunity to canine distemper.
A simple and rapid (24-h) assay for peripheral blood lymphocyte-associated immunity to canine distemper virus (CDV) is described. The test is based upon leukocyte-associated inhibition of CDV-induced syncytia formation in Vero cells. The technique quantitates the response morphologically, thereby eliminating the requirement for release of radiolabeled compounds. Positive results were determined from specific-pathogen-free and gnotobiotic dogs exposed to CDV via hyperimmunization, vaccination with a modified live virus vaccine, and after virulent virus infection. Preinoculation lymphocytes and lymphocytes from non-immune dogs did not inhibit CDV-induced syncytia formation. Maximum responses were observed 7 to 21 days after initial exposure and declined thereafter. The method can be used to further investigate the role of immune lymphocytes in the recovery from CDV infection.
D000818:Animals; D000914:Antibodies, Viral; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004216:Distemper / Q000276:immunology*; D004217:Distemper Virus, Canine / Q000276:immunology*; D004285:Dogs; D008214:Lymphocytes / Q000276:immunology*; D009500:Neutralization Tests; D014611:Vaccination; D014765:Viral Vaccines
D000914:Antibodies, Viral; D014765:Viral Vaccines