Analysis of RNA synthesized in isolated nuclei of Ehrlich ascites tumor cells.
The molecular size and poly-A content of RNA synthesized in isolated nuclei of Ehrlich ascites tumor cells were measured. KCl was found to be essential for synthesis of high molecular weight RNA: when 0.4 M KCl was added to the reaction mixture, the average molecular size of the RNA formed was 14S; without KCl the average molecular size was 5S. A significant amount of poly-A sequences was found in RNA synthesized in the presence of alpha-amanitin, suggesting that RNA polymerase I and/or III may synthesized some RNA containing poly-A in isolated nuclei.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D002474:Cell-Free System; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D008297:Male; D051379:Mice; D008970:Molecular Weight; D011061:Poly A / Q000096:biosynthesis*; D011189:Potassium Chloride / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis*; D012318:RNA Polymerase I / Q000378:metabolism; D012320:RNA Polymerase III / Q000378:metabolism; D012335:RNA, Ribosomal / Q000096:biosynthesis
D000546:Amanitins; D012335:RNA, Ribosomal; D011061:Poly A; D012313:RNA; D011189:Potassium Chloride; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I; D012320:RNA Polymerase III