title: Cell to cell relationships in the seminiferous epithelium in the mouse embryo.
abstract: Germ cells and Sertoli cells in embryonic mouse testes (day 14 to 20 of gestation) were examined by sectioning and freeze-fracture. Intercellular cytoplasmic bridges between the germ cells are observed in day 14 and older embryos. Membrane specializations with dense fuzzy material similar to the so-called "desmosome-like structures" are found between Sertoli cells and germ cells. A cell contact area with dense opposed membranes is also found between adjacent germ cells. Asymmetrical dense fuzzy lining of both Sertoli and germ cell membranes is noted. Pinocytotic pits or caveolae are frequently found in the Sertoli cell membrane. Between adjacent Sertoli cells, gap junctions of various sizes and focal meshworks of the occluding junctions are found. Most of the occluding junctional particles are located in the center of the grooves in the E face, and are similar to those in postnatal and adult Sertoli cell junctions. In addition, on both fractured faces there are ridges and grooves devoid of particles which are continuous with occluding junctions with particles, suggesting an initial stage in the formation of occluding junctions of the Sertoli cells. Particles gathered at the site of desmosome-like structures are present on the P face of the Sertoli cell.
mesh_terms: D000818:Animals; D005614:Freeze Fracturing; D007365:Intercellular Junctions / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D012670:Seminiferous Epithelium / Q000196:embryology* / Q000648:ultrastructure; D012708:Sertoli Cells / Q000648:ultrastructure*; D013094:Spermatozoa / Q000648:ultrastructure*; D013737:Testis / Q000196:embryology*