Structure of chromatin containing extensively acetylated H3 and H4.
I have grown HeLa cells in 5 mM sodium n-butyrate leading to extensive in vivo histone acetylation, and have characterized the structure of chromatin containing the modified histones. Nuclear DNA in butyrate-treated cells is digested 5-10 fold more rapidly by DNAase I than the DNA of control cells. Staphylococcal nuclease degrades the two nuclear samples to acid-soluble material with identical rates; this nuclease, however, does excise nucleosomes with extensively acetylated histones from the nucleoprotein chain preferentially. The physical properties of unsheared chromatin and isolated core particles from control and butyrate-treated cells are closely similar, as are the rates of digestion of core particles from the two cell preparations by DNAase I. Determination of the relative susceptibilities of cleavage sites for DNAase I demonstrates that the site 60 bases from the ends of the DNA resistant in control cells, becomes susceptible to the nuclease in core particles containing acetylated histones. Similarly, the 5' terminal phosphate at the end of DNA in core prticles is removed by staphylococcal nuclease 2-3 fold faster in particles containing acetylated histones than in particles from control cells.
D000107:Acetylation; D002087:Butyrates / Q000494:pharmacology; D002467:Cell Nucleus / Q000032:analysis; D002843:Chromatin / Q000032:analysis*; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006358:Hot Temperature; D009690:Nucleic Acid Conformation; D011487:Protein Conformation
D002087:Butyrates; D002843:Chromatin; D006657:Histones; D004247:DNA; D003851:Deoxyribonucleases