[Effect of estrogens on reconstructed nucleohistones].
The action of estron, 17beta-estradiol and estriol on the extent of histone binding with DNA in the reconstituted nucleohistones was studied; also the influence of these hormones on the thermal denaturation of nucleoproteins was investigated. As revealed, estrogens in a concentration of 10(-5) M failed to alter the protein/DNA weight ratio in the patially reconstituted nucleohistones F1, F2a, F2b, F3 and in the sum total nucleohistone reconstituted from the DNA and the equimolar complex of histones F2a, F2b, F3. Treatment with estrogens in the mentioned concentration did not alter the melting point of all the nucleohistones under study. The influence of estrogens on the thermal denaturation of the sum total nucleohistone and of the partially reconstituted nucleohistones F2a, F2b, and F3 consisted of the enhancement of hyperchromic effect of the first melting phase and a corresponding reduction of hyperchromicity of the rest of the phases. Estrogens failed to alter the heat denaturation profile of the partially reconstructed nucleohistone F1.
D004247:DNA; D004958:Estradiol; D004964:Estriol; D004967:Estrogens; D004970:Estrone; D006657:Histones; D009698:Nucleoproteins; D011489:Protein Denaturation
D004967:Estrogens; D006657:Histones; D009698:Nucleoproteins; D004970:Estrone; D004958:Estradiol; D004247:DNA; D004964:Estriol
Deĭstvie éstrogenov na rekonstruirovannye nukleogistony.