A radiochemical assay for glycine N-acyltransferase activity. Some properties of the enzyme in rat and rabbit.
We have developed a sensitive radiochemical assay of glycine N-acyltransferase activity, using phenylacetyl-CoA as the acyl donor and glycine as the acceptor. This assay measures formation of the product, phenylacetylglycine, instead of disappearance of the substrate, phenylacetyl-CoA, as did earlier assays. The subcellular location and some properties of the conjugating activity were determined in liver and kidney of the rabbit and the rat. Rabbit lung and intestine were also tested for activity.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D005260:Female; D005998:Glycine; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D011817:Rabbits; D011854:Radiochemistry; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D000217:Acyltransferases; C020680:glycine acyltransferase; D005998:Glycine