Influence of monoethanolamine on activity measurements of the isoenzymes of alkaline phosphatase.
Monethanolamine, a frequent impurity of diethanolamine, inhibits the activity of the isoenzymes of alkaline phosphatase to various extents. Isoenzymes from liver and bone, in particular, are strongly inhibited. Inhibition is stronger at lower (25 degrees C) than at higher temperatures (37 degrees C).
D000469:Alkaline Phosphatase / Q000097:blood*; D001842:Bone and Bones / Q000201:enzymology; D002021:Buffers; D002849:Chromatography, Gas / Q000379:methods; D004983:Ethanolamines / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008099:Liver / Q000201:enzymology; D010783:Photometry / Q000379:methods; D013696:Temperature
D002021:Buffers; D004983:Ethanolamines; D007527:Isoenzymes; D000469:Alkaline Phosphatase