[Analysis of brain chromatin subunit organization].
Autodigestion of chromosomal DNA does not take place during the brain nuclei incubation in the presence of Ca2+ and Mg2+. The kinetic of chromatin digestion in brain and liver nuclei by staphylococcal nuclease and the formation of DNP-fragments suggest that subnucleosomes are generated in both cases by digesting of monomer specific sites. This monomer contains 185--200 DNA base pairs and the most starting DNA going throughout it. However the quantity of nuclease-resistant DNA in brain chromatin is more and the rate of subnucleosome formation is less than in liver chromatin. Redigestion of isolated monomers of brain chromatin results in the appearance of subnucleosomes similar to those which are formed under limited digestion of nuclear chromatin. The incubation of brain nuclei in the presence of Ca, Mg-dependent endonuclease prepared from liver nuclei results in the appearance of fragment. DNA-spectra of these fragments are similar to those prepared under digestion of liver chromatin in situ. These data suggest definite resemblance of subunit organization in brain and liver chromatin.
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D002118:Calcium / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000032:analysis*; D004720:Endonucleases; D008099:Liver / Q000648:ultrastructure; D046911:Macromolecular Substances; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008836:Micrococcal Nuclease; D051381:Rats
D002843:Chromatin; D046911:Macromolecular Substances; D004720:Endonucleases; D008836:Micrococcal Nuclease; D008274:Magnesium; D002118:Calcium
Analiz subdedinichnoí organizatsiikhromatina mozga.