Rabbit IgM: its isolation in high yield by a convenient procedure using serum from trypanosome-infected animals.
Rabbit sera contain little IgM. This is a major obstacle for its isolation. However, levels of IgM up to 10 mg/ml are found in rabbits infected with living trypanosomes. A simple procedure, involving gel filtration, standing in the cold, and agarose block electrophoresis is described which allows the isolation of IgM from these sera in 60% yield, with a purity of better than 90%.
D000818:Animals; D002850:Chromatography, Gel; D004587:Electrophoresis, Agar Gel; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007075:Immunoglobulin M / Q000302:isolation & purification*; D011817:Rabbits; D014352:Trypanosomiasis / Q000276:immunology*
D007075:Immunoglobulin M