title: A stage-restricted secretory system in the submandibular gland of the neonatal rat.
abstract: Ribonuclease and amylase have been shown to undergo a transient increase in activity in the submandibular gland of the perinatal rat [5]. We report here that brief stimulation with isoproterenol in vitro selectively releases these enzymes. In addition, analysis by polyacrylamide gel electrophoresis showed that the 4-day old submandibular gland contains three other major protein species that are not present in the sublingual gland and two of which are not evident in the adult submandibular gland. These are also selectively released by the drug. Examination of the glands by light and electron microscopy showed that concurrent with release of these protein products, extensive degranulation occurs in the immature acini (terminal tubules). Our experiments suggest that the 4-day glands show a marked incorporation of 3H-leucine into the submandibular-specific, secreted protein species. This indicates that these proteins will provide convenient molecualr markers of early submandibular differentiation.
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D004589:Electrophoresis, Disc; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D010306:Parotid Gland / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D013363:Submandibular Gland / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
chemical_list: D012260:Ribonucleases; D000681:Amylases; D007545:Isoproterenol