Further improvements in the hepatocyte primary culture DNA repair test for carcinogens: detection of carcinogenic biphenyl derivatives.
DNA repair in hepatocyte primary cultures was induced by simultaneous treatment with the carcinogen and [3H] thymidine for 18 h beginning immediately after attachment of cells. The unscheduled DNA synthesis elicited by carcinogens was determined by counting grains with an automatic grain counter. In 5 biphenyl derivatives, a correlation was found between carcinogenicity and the ability to induce DNA repair. Hence, the method offers promise as a means of screening chemical carcinogens.
D001713:Biphenyl Compounds / Q000494:pharmacology*; D002273:Carcinogens; D002460:Cell Line; D004260:DNA Repair; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D008099:Liver / Q000378:metabolism
D001713:Biphenyl Compounds; D002273:Carcinogens