title: Tissue culture cytotoxicity assay for cyclophosphamide metabolites in rat body fluids.
abstract: An in vitro cytotoxicity assay for cyclophosphamide metabolites in rat body fluids is described. Of the two tissue culture tumor cell lines employed, the Walker-256 rat carcinosarcoma was more sensitive to metabolite levels than the L-1210 mouse lymphocytic leukemia. The Walker-256 system detected cyclophosphamide metabolite levels two orders of magnitude lower than the commonly used 4-(p-nitrobenzyl)pyridine analytical procedure.
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001826:Body Fluids / Q000032:analysis*; D002279:Carcinoma 256, Walker; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000378:metabolism* / Q000633:toxicity; D005260:Female; D007939:Leukemia L1210; D008297:Male; D051381:Rats
chemical_list: D003520:Cyclophosphamide