Ayalysis of translational parameters in cultured cells.
A method is described for the measurement of protein synthesis parameters in cultured cells. Ribosome transit times, polyribosome size distribution and relative synthetic rates are measured on individual cultures of cells. This method is applied to an analysis of cycloheximide (1 micrometer) inhibition of protein synthesis in cultured mouse hepatoma cells.
D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D007700:Kinetics; D008722:Methods; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D012270:Ribosomes / Q000378:metabolism*
D003513:Cycloheximide