Calcium-dependent lipolytic acyl-hydrolase activity in purified plant mitochondria.
Ageing of isolated potato mitochondria induced by CaCl2 resulted in rapid enzymatic hydrolysis of the membrane phospholipids with the liberation of free fatty acids. The enzyme responsible for this effect was identified as a membrane bound lipolytic acyl-hydrolase which was unmasked by CaCl2. The presence of this lipolytic acyl-hydrolase induced severe functional impairments in the mitochondrial oxidative and phosphorylative properties.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008566:Membranes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology*; D010101:Oxygen Consumption; D010715:Phosphatidylglycerols / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D014675:Vegetables
D005227:Fatty Acids; D010715:Phosphatidylglycerols; D010743:Phospholipids; D013386:Succinates; D000244:Adenosine Diphosphate; D002265:Carboxylic Ester Hydrolases; D002118:Calcium