The spectrin phosphorylation reaction in human erythrocytes.
Phosphorylation of spectrin in whole cells occurs on a single tryptic peptide and indicates a specific and limited modification of spectrin. Phosphorylation of spectrin in soluble extracts and isolated membranes is less specific. Diamide is an inhibitor of erythrocyte spectrin kinase and casein kinase. This agent also inhibits the restoration of biconcave morphology to metabolically depleted erythrocytes reincubated with adenosine.
D000255:Adenosine Triphosphate / Q000097:blood; D001391:Azo Compounds / Q000494:pharmacology*; D003958:Diamide / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004913:Erythrocytes, Abnormal / Q000187:drug effects; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000097:blood*; D013049:Spectrin / Q000378:metabolism*
D001391:Azo Compounds; D008565:Membrane Proteins; D047428:Protein Kinase Inhibitors; D003958:Diamide; D013049:Spectrin; D000255:Adenosine Triphosphate; D011494:Protein Kinases