Evidence from UV transcription mapping that late adenovirus type 2 mRNA is derived from a large precursor molecule.
The UV inactivation of labeling of various late adenovirus type 2 mRNA's is as great or greater than the inactivation of labeling of the same sequences that are present in a large primary RNA transcript in the cell nucleus. Thus, the large nuclear adenovirus type 2-specific RNA appears to be the obligatory precursor of cytoplasmic mRNA. The majority of the mRNA's derived from the large precursor apparently require the synthesis of the whole nuclear molecule. A UV-sensitive target necessary for proper processing of all the mRNA's may exist near the end of the transcription unit.
D000260:Adenoviruses, Human / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004279:DNA, Viral / Q000528:radiation effects*; D006367:HeLa Cells; D009694:Nucleic Acid Precursors / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000528:radiation effects*; D014466:Ultraviolet Rays
D004279:DNA, Viral; D009694:Nucleic Acid Precursors; D012333:RNA, Messenger; D012367:RNA, Viral