Dipeptidyl aminopeptidase IV, a glycoprotein from pig kidney.
Dipeptidyl aminopeptidase IV was purified 350 fold from pig kidney by chromatographic procedures including affinity chromatography with conjugates of Gly-Pro linked to Sepharose 4.B. Purified enzyme existed in a dimeric form as determined by sodium dodecyl sulfate polyacrylamide-gel electrophoresis using dimethyl suberimidate (a cross-linking reagent). The molecular weight of the subunit was estimated to be 100 000 by gel filtration with 6 M guanidine hydrochloride and to be 94 000 based on analysis of N-terminal residue (dinitrophenyl-serine). The amino acid composition of the purified enzyme was also determined. The enzyme contained 18.3% of carbohydrate consisting of mannose, galactose, fucose, glucosamine and sialic acid. The enzyme desialized with sialidase was found to still possess full enzyme activity.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D004151:Dipeptides; D010450:Endopeptidases / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D007668:Kidney / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D013552:Swine
D000596:Amino Acids; D002241:Carbohydrates; D004151:Dipeptides; D006023:Glycoproteins; D046911:Macromolecular Substances; D010450:Endopeptidases