Determination of 1,2-benzisoxazole-3-acetamidoxime hydrochloride (PE-257) in plasma using electron-capture gas chromatography.
A gas chromatographic method has been developed that permits the accurate and specific determination of a new psychotropic agent, PF-257, in plasma. PF-257 is extracted with ethyl acetate from alkaline plasma and, after a clean-up procedure, derivatized with heptafluorobutyric anhydride to form 3-[(5-n-heptafluoropropyl-1,2, 4-oxadiazol-3-yl)methyl]-1,2-benzisoxazole (HOMB). The HOMB is assayed on a gas chromatograph equipped with an electron-capture detector. Accurate determinations of PF-257 are possible in the concentration range from 1-40 ng/ml with a relative standard deviation of 6.8%. The minimum detectable concentration in plasma is 0.1 ng/ml. Plasma levels of PF-257 in rats receiving intravenous or oral dosing (10 mg/kg) were determined.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D007555:Isoxazoles / Q000097:blood*; D010080:Oxazoles / Q000097:blood*; D010091:Oximes / Q000097:blood; D011619:Psychotropic Drugs / Q000097:blood*; D051381:Rats
D007555:Isoxazoles; D010080:Oxazoles; D010091:Oximes; D011619:Psychotropic Drugs; C006076:PF 257