The structure of carbohydrate chains of blood-group substance. Isolation and elucidation of the structure of higher oligosaccharides from blood-group substance H.
Twenty individual higher reduced oligosaccharides, having from seven to eleven monosaccharide units, were isolated after sodium borohydride degradation of blood-group substance H from pig stomach linings. Anion-exchange high-pressure liquid chromatography appears to be a very convenient and effective method for this kind of higher oligosaccharide mixtures separation. The oligosaccharide structures were determined by means of periodate oxidation, methylation analysis, partial acid and enzymic hydrolysis. It has been found that all the oligosaccharides investigated can be divided into four series. The oligosaccharides belonging to each series have the common oligosaccharide fragment to which terminal L-fucose and/or N-acetyl-D-glucosamine residues are attached. Comparison of all the oligosaccharide structures, including tri, penta and hexasaccharides described earlier, shows that the lower oligosaccharides represent the structural element of the higher oligosaccharides.
D000017:ABO Blood-Group System; D000116:Acetylgalactosamine / Q000032:analysis; D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D008722:Methods; D008968:Molecular Conformation; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification; D013552:Swine
D000017:ABO Blood-Group System; D009844:Oligosaccharides; D005643:Fucose; D000116:Acetylgalactosamine; D000117:Acetylglucosamine; D005690:Galactose