title: Transfer from Rhizobium japonicum to Azotobacter vinelandii of genes required for nodulation.
abstract: A mutant strain of Azotobacter vinelandii that is unable to fix N2 (Nif-) was transformed to Nif+ with DNA from Rhizobium japonicum. Of 50 Nif+ transformants tested, 3 contained the O antigen-related polysaccharide that is present on the cell surface of a nodulating R. japonicum strain, but is absent from a non-nodulating mutant strain.
mesh_terms: D000372:Agglutination Tests; D000942:Antigens, Bacterial / Q000032:analysis; D001395:Azotobacter / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005796:Genes; D009154:Mutation; D009586:Nitrogen Fixation; D011135:Polysaccharides, Bacterial / Q000032:analysis; D012231:Rhizobium / Q000235:genetics* / Q000276:immunology; D014169:Transformation, Bacterial
chemical_list: D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial