title: Autoradiographic study of RNA synthesis during meiotic prophase in the human oocyte.
abstract: The incorporation of 3H-uridine in oogonia and oocytes during meiotic prophase I was studied in three human fetuses 13, 18, and 19 weeks old. Following a 40- or 60-min pulse, intense nuclear and nucleolar labeling was observed in oogonia. During the preleptotene chromosome condensation stage, the heteropycnotic masses were unlabeled, while numerous silver grains were seen on the filaments persisting around these masses. During leptotene, chromosomal and nucleolar RNA synthesis was significant, but less than that in the oogonia. The rate of incorporation declined rapidly during zygotene and fell to a very low level at early pachytene. Throughout pachytene no nucleolar RNA synthesis was observed. Chromosomal RNA synthesis progressively recovered during middle pachytene, was of moderate intensity at late pachytene, and increased again at early diplotene. Nucleolar RNA synthesis was very intense at early diplotene, at the same time as nucleolar size and basophilia increased.
mesh_terms: D001345:Autoradiography; D002877:Chromosomes, Human; D005260:Female; D006801:Humans; D008540:Meiosis; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D009867:Oogonia / Q000166:cytology / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000196:embryology; D010063:Ovum / Q000166:cytology*; D012313:RNA / Q000096:biosynthesis*
chemical_list: D012313:RNA