Preparative polyacrylamide gel electrophoresis of nuclear RNA.
A method is described for the separation of nuclear RNA by preparative polyacrylamide gel electrophoresis. Separation is performed on a slab gel. The elution chamber system is rectangular. Total nuclear RNA can be fractionated into the prominent RNA species 4 - 7S, 18S and 28S RNA. The less prominent RNA, especially the high molecular weight precursor molecules of ribosomal RNA 45S (Mr = 4.1 X 10(6)) and 32S (Mr = 2.1 X 10(6)) can be separated from the total nuclear RNA without contamination by other RNA. The time needed for a preparative run is 260 min.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008970:Molecular Weight; D012334:RNA, Neoplasm / Q000302:isolation & purification*
D012334:RNA, Neoplasm