[Determination of progesterone and delta 4-pregnen-20 alpha-ol-3-one contents in ovaries with two-dimensional thin-layer chromatography and spectrofluorimetry].
The method is developed for quantitative determination of progesterone and delta4-pregnen-20alpha-ol-3-one in ovaries. It includes treatment of the gland homogenate with NaOH, ethylacetate extraction, two-dimensional thin-layer chromatography on silicaged plate Silufol UV254 in benzol-ethylacetate (80:20) and chlorophorm-ethanol (98:2) and the subsequent spectrofluorimetry. For identification of the isolated steroids their chromatographical motility, light absorption, color reactions and fluorescence spectra were studied. The contents of progesterone and delta4-pregnen-20alpha-ol-3 one in the rat ovaries were determined by this method.
D000818:Animals; D002855:Chromatography, Thin Layer; D005260:Female; D007536:Isomerism; D008722:Methods; D010053:Ovary / Q000032:analysis*; D011374:Progesterone / Q000031:analogs & derivatives* / Q000032:analysis*; D051381:Rats; D013050:Spectrometry, Fluorescence
D011374:Progesterone
Opredelenie soderzhaniia progesterona i del'ta 4-pregnen-20al-fa-ol-3-ona v iaichnikakh posredstvom dvukhmernoĭ tonkosloinoĭ khromatografii i spektrofliuopimetrii.