New electron-capture gas-liquid chromatographic method for the determination of mexiletine plasma levels in man.
A method for the determination of mexiletine in human plasma by gas-liquid chromatography with electron-capture detection is described. Plasma samples are extracted at pH 12 with dichloromethane after addition of the internal standard, the 2,4-methyl analogue of mexiletine. A derivative is obtained using heptafluorobutyric anhydride; according to gas chromatography-mass spectrometry it is a monoheptafluorobutyryl compound. The minimum detectable amount of mexiletine is 5 pg. Accurate determinations of human plasma levels were performed after oral or intravenous treatment.
D000284:Administration, Oral; D002849:Chromatography, Gas / Q000379:methods; D002853:Chromatography, Liquid; D006801:Humans; D007275:Injections, Intravenous; D008801:Mexiletine / Q000097:blood* / Q000652:urine; D011437:Propylamines / Q000097:blood*
D011437:Propylamines; D008801:Mexiletine