A high performance liquid chromatographic system for the analysis of tetracycline drug standards, analogs, degradation products and other impurities.
This paper describes the high performance liquid chromatographic (HPLC) analysis of eight parent tetracycline standards: tetracycline, chlortetracycline, rolitetracycline, oxitetracycline, minocycline, doxycycline, democlocycline, methacycline; and three tetracycline epimers: epitetracycline, epianhydrotetracycline, and anhydrotetracycline. The HPLC system employs an octadecylsilane reverse phase column and an isopropanol-diethanolamine-phosphate-ammonium EDTA-water mobile phase. This system produced at least partial resolution of all eight parent compounds and many of their degradation products.
D002751:Chlortetracycline / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004318:Doxycycline / Q000032:analysis; D008690:Methacycline / Q000032:analysis; D008911:Minocycline / Q000032:analysis; D010118:Oxytetracycline / Q000032:analysis; D012016:Reference Values; D012382:Rolitetracycline / Q000032:analysis; D013237:Stereoisomerism; D013752:Tetracycline / Q000032:analysis; D013754:Tetracyclines / Q000032:analysis*
D013754:Tetracyclines; D013752:Tetracycline; D008911:Minocycline; D012382:Rolitetracycline; D008690:Methacycline; D004318:Doxycycline; D002751:Chlortetracycline; D010118:Oxytetracycline