[Properties of the rat liver DNA-dependent RNA-polymerase stored at -25 degrees C in the presence of glycerol or polyethyleneglycol-400].
The effect of slow freezing (1-2 degrees C/min) was studied as applied to the template activity of DNA in the RNA-polymerase reaction. It was found out that the activity of each RNA-polymerase form decreases after 24-hour storage by freezing at -25 degrees C in the presence of glycol or polyethylene glycol-400. The study of the enzyme in the glycerol concentration gradient showed sedimentation constant changes.
D000818:Animals; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004356:Drug Storage; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats
D011092:Polyethylene Glycols; D012321:DNA-Directed RNA Polymerases; D005990:Glycerol
Svoĭstva DNK-zavisimoĭ RNK-polimerazy iz pecheni frys posle khraneniia pri minus 25 gradusov C v prisutstvii glutserina i polietilenglikolia-400.