Quantitative determination of acetaminophen in plasma.
A simple method is described for the rapid quantitative analysis of acetaminophen in plasma. The acetaminophen and its conjugates present in the plasma following drug administration are hydrolyzed with 4 N HCl to p-aminophenol. This compound is coupled with 5% vanillin reagent to form a stable yellow color whose concentration is determined spectrophotometrically at 395 nm. Application of this method to a study of three dogs treated with 650 mg of acetaminophen is described.
D000082:Acetaminophen / Q000097:blood*; D000818:Animals; D004285:Dogs; D008027:Light; D008722:Methods; D013053:Spectrophotometry
D000082:Acetaminophen