Separation of two conjugates of clofibric acid (CPIB) found in the urine of subjects taking clofibrate.
1. Two main conjugates of CPIB (2-[chlorophenoxy]-2-methylpropionic acid) are present in the urine of subjects taking clofibrate. The metabolites can be separated by thin-layer chromatography (TLC). 2. Both conjugates are hydrolysed by dilute alkali, but only one is hydrolysed by the enzyme beta-glucuronidase. In eighty-five urine specimens this conjugate accounted for an average of 54.5% (range 25-70%) of the total CPIB, while 2.6-12.45% (mean 5.1%) was present as free CPIB.
D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D002994:Clofibrate / Q000302:isolation & purification / Q000652:urine*; D005965:Glucuronates / Q000652:urine; D005966:Glucuronidase; D006801:Humans; D006868:Hydrolysis
D005965:Glucuronates; D005966:Glucuronidase; D002994:Clofibrate