Detection and determination of N-nitrosamino acids by thin-layer chromatography using fluorescamine.
A novel procedure is described for the detection and determination of N-nitrosamino acids (NAAs) on activated silica gel thin-layer chromatographic plates. N-Nitrososarcosine, N-nitrosoproline, and N-nitroso-4-hydroxyproline could be detected as fluorophors at the 200-pmole level (20-30 ng) after being irradiated with ultraviolet light and sprayed with fluorescamine reagent. Spectrophotometric determination of the relative fluorescence of 0.4-40 nmoles of NAAs gave rise to similar calibration curves when plotted on a log-log scale. An application of this method to the detection of NAAs in uncooked bacon is described.
D000596:Amino Acids / Q000032:analysis*; D002855:Chromatography, Thin Layer; D005450:Fluorescamine; D006909:Hydroxyproline / Q000031:analogs & derivatives / Q000032:analysis; D008461:Meat Products / Q000032:analysis; D009602:Nitrosamines / Q000032:analysis*; D011392:Proline / Q000031:analogs & derivatives / Q000032:analysis; D012521:Sarcosine / Q000031:analogs & derivatives / Q000032:analysis; D013050:Spectrometry, Fluorescence
D000596:Amino Acids; D009602:Nitrosamines; C011317:N-nitroso-4-hydroxyproline; D005450:Fluorescamine; D011392:Proline; C010259:nitrosoproline; C013162:N-nitrososarcosine; D006909:Hydroxyproline; D012521:Sarcosine