Automatic column chromatographic analysis of saccharides and uronic acids.
Using n-proponal/water as the eluting solvent, a flow rate of 50 ml/h and a 90 degrees C column temperature, the separation of a complex mixture of 11 monosaccharides could be much improved over the procedure described by v. Wilm and Sordé using isopropanol/water, 70--100 ml/h flow rate and temperatures of 83--90 degrees C. With a small total error of 3%, the saccharide analysis lies in the region of reproducibility that has also been reached in amino acid analysis. The limit of detection for saccharides lies at 1 x 10(-9) mole/ml.
D001322:Autoanalysis; D002845:Chromatography / Q000379:methods; D004187:Disaccharides / Q000032:analysis*; D006801:Humans; D009005:Monosaccharides / Q000032:analysis*; D014574:Uronic Acids / Q000032:analysis*
D004187:Disaccharides; D009005:Monosaccharides; D014574:Uronic Acids