Development mutants of Streptomyces coeruleorubidus, a producer of anthracyclines: isolation and preliminary characterization.
The wild strain Streptomyces coeruleorubidus JA 10092 was found to segregate into two spontaneous morphological variants (spo-1 and bld-1) with a different ability to form aerial mycelium in media with glucose as the main carbon source. Six new types of developmental mutants were obtained from the bald variant bld-1 after treatment with mutagens (UV light, gamma radiation, nitrous acid) and after natural selection. Formation of the aerial mycelium was fully suppressed in the bld-2 type growing on media both with glucose and with starch. The other types were bald only on starch media, forming the aerial mycelium on media with glucose; types spo-2, spo-3, spo-4 and spo-5 differed in size, shape and surface structure of spores, the type whi formed asporogenous aerial hyphae.
D000617:Aminoglycosides / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D012641:Selection, Genetic; D013171:Spores, Bacterial / Q000254:growth & development; D013213:Starch / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
D000617:Aminoglycosides; D013213:Starch; D005947:Glucose