Two-step modification of aspartate aminotransferase with 1,5-difluoro-2,4-dinitrobenzene. Cross-link localization.
At pH 7, the apoenzyme of carboxymethylated and acylated aspartate aminotransferase reacts selectively with 1,5-difluoro-2,4-dinitrobenzene to form a single intramolecular covalent bond with the epsilon-amino group of the functional lysine residue located within the active centre. On shifting the pH to 9, the second fluorine atom of the bifunctional reagent is substituted with the sterically adjacent side groups of cysteine and tyrosine residues. The modified apoenzyme was subjected to partial proteolysis with pronase, and the digest was used to obtain and isolate the labeled products and to localize amino acid residues involved in the reaction. The established structures of several peptides containing Cys-2,4-dinitrobenzene-Lys and Tyr-2,4-dinitrobenzene-Lys allowed the identification of the amino acid residues involved in the reaction with the bifunctional reagent as Lys 258, Cys 390 and probably Tyr-70. The residues of Cys and Tyr are thus located at a distance of approximately 5 A (the length of the dinitrophenylene bridge) from the lysine residue forming an aldimine bond with pyridoxal 5'-phosphate in the active site.
D000818:Animals; D001219:Aspartate Aminotransferases; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives; D009206:Myocardium / Q000201:enzymology; D009578:Nitrobenzenes / Q000031:analogs & derivatives; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D013552:Swine
D009578:Nitrobenzenes; D010446:Peptide Fragments; D004139:Dinitrofluorobenzene; D001219:Aspartate Aminotransferases