title: [Soluble high molecular weight derivatives of pancreatic trypsin inhibitor. Isolation and properties of the inhibitor bound to carboxymethyl cellulose and diethylaminoethyl dextran].
abstract: Soluble preparations of pancreatic trypsin inhibitor, which is bound to carboxymethyl cellulose- and diethylaminoethyl dextran activated by chlorine derivatives of s-triazine were isolated. The activity of the pancreatic inhibitor bound to carboxymethyl cellulose treated by 2-amino-4,6-dichloro-s-triazine was completely retained. Simultaneously, the reaction cyanuric chloride activation of water-soluble polysaccharide carriers in a non-aqueous solvent was studied by assaying the amount of chlorine atoms on the treated matrix. The gel-filtration experiments show that the cross-linkages between polysaccharide molecules appear during the activation reaction. A structural model of a carboxymethyl cellulose-bound pancreatic inhibitor is proposed.
mesh_terms: D002266:Carboxymethylcellulose Sodium; D003911:Dextrans; D008970:Molecular Weight; D011485:Protein Binding; D011487:Protein Conformation; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000302:isolation & purification; D014361:Trypsin Inhibitors / Q000302:isolation & purification
chemical_list: D003911:Dextrans; D014361:Trypsin Inhibitors; D014359:Trypsin Inhibitor, Kazal Pancreatic; D002266:Carboxymethylcellulose Sodium
vernacular_title: Rastvorimye vysokomolekuliarnye proizvodnye pankreaticheskogo ingibitora tripsina. Poluchenie i svoistva pankreaticheskogo ingibitora, sviazannogo s karboksimetiltselliulozoĭ i dietilaminoetildekstranom.