Role of cell shape in growth control.
Tissue culture plastic adhesivity was precisely varied by applying different concentrations of poly(2-hydroxyethyl methacrylate). The extent of cell spreading was thus accurately controlled so that cells cultured on these substrata could be held at any one of a graded series of quantitated cell shapes. Cell shape was found to be tightly coupled to DNA synthesis and growth in nontransformed cells. These findings suggest a mechanism that is important in growth control of mammalian cells, and provide a more fundamental interpretation of such phenomena as density dependent inhibition of cell growth and anchorage dependence.
D002448:Cell Adhesion; D002453:Cell Cycle; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D002478:Cells, Cultured / Q000166:cytology* / Q000378:metabolism; D003260:Contact Inhibition; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D006133:Growth Substances / Q000097:blood; D011102:Polyhydroxyethyl Methacrylate
D003470:Culture Media; D006133:Growth Substances; D011102:Polyhydroxyethyl Methacrylate; D004247:DNA