Cell-mediated immunity in the lungs of rabbits using conjugated proteins.
A study using direct macrophage migration inhibition of cells from the lower respiratory tract of rabbits immunized with conjugated proteins, incorporated into a killed BCG-oil emulsion, revealed that the reaction was largely carrier specific. The only exception was when bronchoalveolar cells (BAC) from animals immunized with dinitrophenol coupled to ovalbumin (DNP-OA) were significantly immobilized with DNP coupled to bovine gamma-globulin (BGG) in which the ratio of DNP/BGG was high [DNP-BGG(H)]. This result could not be explained by a toxic effect or by a high net negative charge on DNP-BGG(H). In addition, inhibition of BAC from DNP-OA-immunized rabbits was not observed when the ratio of DNP to BGG was reduced approximately 5-fold.
D000818:Animals; D002352:Carrier Proteins / Q000276:immunology*; D002464:Cell Migration Inhibition; D005260:Female; D006241:Haptens; D007111:Immunity, Cellular; D008168:Lung / Q000276:immunology* / Q000473:pathology; D008297:Male; D011817:Rabbits
D002352:Carrier Proteins; D006241:Haptens