Measuring IgG anti-A/B titres using dithiothreitol (DTT).
In comparing the use of the sulphydryl compounds 2-mercaptoethanol (2ME) and dithiothreitol (DTT) a rapid one-stage technique for titrating IgG anti-A and anti-B using DTT in an antiglobulin test was tried. The results correlated well with those obtained by either of the two-stage methods using 2ME or DTT.
D000017:ABO Blood-Group System; D003298:Coombs Test; D004229:Dithiothreitol; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M; D007518:Isoantibodies / Q000032:analysis*; D008623:Mercaptoethanol
D000017:ABO Blood-Group System; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies; D008623:Mercaptoethanol; D004229:Dithiothreitol