[High-yield isolation of chloroplast DNA from Hordeum vulcare barley protoplasts].
A fraction of intact chloroplasts free of other cell components in isolated from barley leaf chloroplasts. Instead of mechanical desintegration of plant tissue, the method described includes the cellulysine treatment, thus increasing the yeild of chloroplast DNA. The mean content of DNA per barley chloroplast is found to be 1.10(-4) g. Base composition of barley chloroplast DNA is 39.8 mol.% of G+C. The treatment of chloroplast DNA with restriction endonuclease EcoRI results in the appearance of 17-19 bands under agarose gel electrophoresis.
D002482:Cellulose / Q000031:analogs & derivatives / Q000494:pharmacology; D002734:Chlorophyll / Q000032:analysis; D002736:Chloroplasts / Q000032:analysis* / Q000187:drug effects; D004247:DNA / Q000302:isolation & purification*; D002523:Edible Grain / Q000648:ultrastructure*; D004587:Electrophoresis, Agar Gel; D001467:Hordeum / Q000648:ultrastructure*; D008722:Methods; D011523:Protoplasts / Q000032:analysis
D002734:Chlorophyll; D002482:Cellulose; D004247:DNA
Vydelenie khloroplastnoĭ DNK s bol'shim vykhodom iz protoplastov iachmenia Hordeum vulcare.