A sensitive and specific determination of gamma aminobutric acid in brain.
A sensitive and specific method for the determination of gamma aminobutyric acid in small (30 mg) brain samples is described. It is based on preparation of the dansyl-butyrolactam (DBL), subsequent separation by thin layer chromatography and measurement of fluorescence after elution. Recovery is checked by routinely adding 14C labelled GABA.
D000613:Aminobutyrates / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds; D007769:Lactams; D051379:Mice; D051381:Rats
D000613:Aminobutyrates; D003619:Dansyl Compounds; D007769:Lactams