Methotrexate induced alteration of glycolysis in L1210 cells in vitro.
Methotrexate, in non-lethal doses, greatly enhances anaerobic glycolysis in L1210 cells. Lactate/glucose ratio remains constant but intracellular levels of glycolytic enzymes are increased. ATP accumulating in the cells seems to reflect an alteration of regulation of the glycolysis.
D000818:Animals; D002478:Cells, Cultured; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D007939:Leukemia L1210 / Q000378:metabolism*; D008727:Methotrexate / Q000494:pharmacology*; D011770:Pyruvate Kinase / Q000378:metabolism
D007773:Lactates; D005954:Glucosephosphate Dehydrogenase; D011770:Pyruvate Kinase; D005634:Fructose-Bisphosphate Aldolase; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose; D008727:Methotrexate