Radioimmunoassay of 2-hydroxyestrone.
Under the protection of ascorbic acid a 2-hydroxyestrone bovine serum albumin conjugate was prepared containing intact 2-hydroxyestrone as determined by gas chromatographymass spectometry. Using this antigen highely specific antibodies were raised in rabbits. Cross-reactivity for 2-hydroxyestradiol and 2-hydroxyestriol was 26 and 4.5%, respectively. An assay procedure of 2-hydroxyestrone in human plasma is described. Using special precautions the assay allows the determination of 2-hydroxyestrone in plasma samples of women (50-95 pg/ml), pregnant women (105-220 pg/ml), men (45-65 pg/ml) and children(20-40 pg/ml).
D000328:Adult; D000937:Antigen-Antibody Reactions; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D004970:Estrone / Q000031:analogs & derivatives*; D005260:Female; D006801:Humans; D006894:Hydroxyestrones / Q000097:blood*; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods
D006894:Hydroxyestrones; D004970:Estrone