[Use of the method of double labeling with H3 and C14-thymidine for identification of cells in different periods of interphase].
For the identification of the position of individual cells in G1, S- and G2-periods of mitotic cycle in any heteroploid cell culture, it is suggested to use the Wimber and Quastler radioautographical method of double labeling of cells. It was shown that independent of the basal polidy of the cells by the successive impulse labeling of the cells with H3- and C14-thymidine with the time interval as long as G2 + M, the cells of the G1-period are unlabeled, S-cells are double labeled with C14- and C14 + H3, and G2-cells have only H3-label.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002250:Carbon Radioisotopes; D002453:Cell Cycle; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D007399:Interphase; D007553:Isotope Labeling / Q000379:methods*; D013936:Thymidine; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D013936:Thymidine
Ispol'zovanie metoda dvoinogo mecheniia 3H- i 14C-timidinom dlia identifikatsii kletok v raznykh periodakh interfazy mitoticheskogo tsikla.