[Simultaneous enzyme determination of C-14 glucose and C-14 fructose in human body fluids].
A new enzymatic method for simultaneous estimation of radioactivity of 1-14C-glucose and 1-14C-fructose is described. It is based on the isomerisation of 1-14C-fructose to 1-14C-glucose by phosphoglucoseisomerase (EC 5.3.1.9) and on its enzymatic removal as ribulose-5-phosphate and 14CO2. The method is specific, reproducible, and gives over 97% recoveries of glucose and fructose concentrations up to 11 mmol/1 in aquos and biological solutions.
D001826:Body Fluids / Q000032:analysis*; D005632:Fructose / Q000032:analysis*; D005947:Glucose / Q000032:analysis*; D005956:Glucose-6-Phosphate Isomerase; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods
D007202:Indicators and Reagents; D005632:Fructose; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose
Gleichzeitige enzymatische Bestimmung von 14C-Glukose und 14C-fruktose in menschlichen Körperflüssigkeiten.