[Phospholipid distribution and metabolism in the neurons and neuroglia].
Ratio and intensity of metabolism of phospholipid separate fractions were studied in neurons and neuroglia, isolated from brain cortex by differential ultracentrifugation in density gradient of sucrose and Ficoll with the rate of enriching up to 90% and 80%, respectively. Neurons were characterized by the higher ratio of monophosphoinositides and lysophosphatidyl cholines as compared with neuroglia. Content of phosphatide acids and sphingomyelins was higher in neuroglia. The most specific radioactivity was observed in phosphatide acids both in neurons and neuroglia if 2(-14)C-acetate was administered. Neurons were shown to have higher metabolism of the separate fractions of phospholipids.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002540:Cerebral Cortex / Q000378:metabolism; D008055:Lipids / Q000302:isolation & purification; D008297:Male; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010743:Phospholipids / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D014461:Ultracentrifugation
D008055:Lipids; D010743:Phospholipids
Raspredelenie i metabolizm fosfolipidov neĭronov i neĭroglii.