Template activity for histones of poly(A)-minus RNA fraction from different developmental stages of Artemia salina embryos.
Template activity for histones of RNA fractions derived from Artemia salina embryos at different developmental stages were measured in a Krebs ascites cell-free system. Appreciable amounts of acid-soluble polypeptides comigrating with Hela cell histone markers on acrylamide gel electrophoresis were detected only when RNA fractions from nauplii were used. Tryptic peptide analysis by high voltage electrophoresis of the translational products had a pattern qualitatively similar to that of in vivo labeled histone markers from Hela.
D000818:Animals; D002474:Cell-Free System; D033364:Decapoda / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006657:Histones / Q000096:biosynthesis* / Q000302:isolation & purification; D066298:In Vitro Techniques; D011061:Poly A; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D013997:Time Factors
D006657:Histones; D012333:RNA, Messenger; D011061:Poly A