title: Presence of genomic polyadenylate and absence of detectable virion transcriptase in human coronavirus OC-43.
abstract: Human coronavirus RNA, prepared by extraction of purified virions with phenol-chloroform, consists of a major 15 to 55S class and a minor 4S class of RNA fragments. Polyadenylic acid [poly (A)] sequences are present in 15 to 55S but not in 4S RNA, suggesting different functions for each class. A stretch of poly (A) of approximately 19 adenosine monophosphate residues was obtained in sizing experiments after digesting OC-43 RNA with pancreatic and T1 ribonucleases. An OC-43 virion RNA transcriptase could not be detected with systems optimal for detecting the transcriptases of influenza and Newcastle disease virus.
mesh_terms: D001483:Base Sequence; D003332:Coronaviridae / Q000032:analysis* / Q000201:enzymology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D011061:Poly A / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D012367:RNA, Viral; D011061:Poly A; D012321:DNA-Directed RNA Polymerases