A two-stage enzymatic method for determination of uric acid and hypoxanthine/xanthine.
A method is described by which plasma or serum concentrations of uric acid and hypoxanthine/xanthine may be determined in a two-step sequential method. The principle of the method is that hydrogen peroxide, liberated by the action of uricase and xanthine oxidase on their substrates, is coupled to the production of a coloured product.
D006801:Humans; D007042:Hypoxanthines / Q000097:blood*; D007511:Ischemia / Q000097:blood; D008722:Methods; D014503:Urate Oxidase / Q000378:metabolism; D014527:Uric Acid / Q000097:blood*; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000097:blood*
D007042:Hypoxanthines; D014970:Xanthines; D014527:Uric Acid; D014969:Xanthine Oxidase; D014503:Urate Oxidase