title: L-Ascorbic acid and lysosomal acid hydrolase activities of guinea pig liver and brain.
abstract: The effects of L-ascorbic acid deficiency on guinea pig hepatic and brain lysosomal hydrolases were examined. In general, hepatic beta-N-acetylhexosaminidase, beta-D-glucoronidase, alpha-D-galactosidase, alpha-D-mannosidase, and acid phosphatase were elevated in scorbutic animals. This appears to be independent of the starved state. Brain beta-D-glucoronidase and acid phosphatase followed a similar pattern to that observed with the liver enzymes, but brain beta-N-acetylhexosaminidase was not affected by L-ascorbic acid decreased the activity of hepatic beta-N-acetylhexosaminiadase was unaffected by dietary treatments although the activity of beta-N-acetylhexosaminidase A tended to increase in the scorbutic animals. Subcellular fractions were obtained from the three groups of animals and the recoveries of protein, beta-N-acetylhexosaminidase, and glucose-6-phosphatase estimated.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001206:Ascorbic Acid Deficiency / Q000201:enzymology; D001921:Brain / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006168:Guinea Pigs; D006867:Hydrolases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D006867:Hydrolases; D000135:Acid Phosphatase; D006026:Glycoside Hydrolases; D001205:Ascorbic Acid