title: Studies on Crocker sarcoma 180 under the effect of alkylating agents. IV. Morphological studies of sarcoma 180 during treatment with the antitumour drug cytoxan.
abstract: The effect of one massive dose of cytoxan (150 mg/kg), as an alkylating agent, on the morphology of sarcoma 180 cells was followed after 1, 3, 6, 12, 24, 48, 96, 120, and 168 h. After 1 h the cells of treated tumour showed clumping and a decrease in their size. In addition, statistical analysis manifested significant decrease in all phases of mitotic figures. All mitotic figures were completely stopped after 3 h from cytoxan treatment. After 6 and 12 h from cytoxan treatment the hyaline degenerations became more marked in the treated tumour tissue. Chromosomal bridge formation, nuclei aberrations and polynuclear cells were more prominent in 24, 48, and 96 h from cytoxan treatment. Increase in the nuclear size and hyaline degeneration were observed in treated section of 120 and 168 h.
mesh_terms: D000477:Alkylating Agents / Q000627:therapeutic use*; D000818:Animals; D002869:Chromosome Aberrations / Q000187:drug effects; D003520:Cyclophosphamide / Q000627:therapeutic use; D005260:Female; D008297:Male; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D009504:Neutrophils / Q000473:pathology; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000477:Alkylating Agents; D003520:Cyclophosphamide