High-pressure liquid chromatographic analysis of carbofuran and two non-conjugated metabolites in crops as fluorescent dansyl derivatives.
Carbofuran, and non-conjugated 3-hydroxycarbofuran and 3-ketocarbofuran were extracted from carrots, corn and potatoes with acetone and partitioned into hexane-methylene chloride. The organic extract was evaporated to a small volume for clean-up on a 2% deactivated Florisil column. All three carbamates were eluted with 15% acetone in hexane. The pesticide residues were hydrolysed to their corresponding phenols with 0.1 M sodium carbonate followed by derivatization with dansyl chloride in acetone. The derivatives were extracted and analysed by high-pressure liquid chromatography with fluorescence detection (excitation, 360 nm; emission, greater than 400 nm). Absolute recoveries for all three compounds were between 50 and 65% for spiked samples by the extraction method used. Detection limits approached 0.01 ppm in the foods studied.
D002235:Carbofuran / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014675:Vegetables / Q000032:analysis*; D003313:Zea mays / Q000032:analysis*
D003619:Dansyl Compounds; D007306:Insecticides; D002235:Carbofuran