Saccharide alterations in rat kidney associated with malignant transformation by injection of dimethylnitrosamine.
Renal tumours were induced in dietary-primed rats by injection of dimethylnitrosamine. Control and tumour tissue was excised at varying periods and maintained in short-term organ culture in the presence of 3H- or 14C-fucose. The plasma membranes were then isolated, and the isotopic profiles of normal kidney and renal tumour membrane proteins were established, using polyacrylamide-gel electrophoresis in dodecyl sulphate. Several fucose-containing glycoproteins of the plasma membranes were found to alter upon neoplastic transformation: 4 increased and 3 decreased. The probable identity of 2 of these proteins is indicated: alpha-foetoprotein is one of the glycoproteins which increased, whereas neutral endopeptidase decreased in the tumour membranes. Fluorescein-labelled lectin binding by the kidney tissue was also found to alter upon transformation. The most marked changes were an increase in sialic acid (neuraminidase-sensitive) and galactosamine (Ricinus communis agglutinin Type I) in the nuclei of some neoplastic cells and some hyperplastic-tubule cells.
D000818:Animals; D004128:Dimethylnitrosamine; D005260:Female; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D007680:Kidney Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D037102:Lectins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D009924:Organ Culture Techniques; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism
D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins; D012794:Sialic Acids; D005643:Fucose; D004128:Dimethylnitrosamine