Stability of transplanted murine tumour systems after storage of cells at -196 degrees C for up to 13 years.
Two murine lymphomas of spontaneous origin were stored in liquid N2 as cell suspensions in DMSO for 8 and 13 years respectively. Isogenic transplantation assays done some years before freezing and immediately after thawing indicated no measurable loss of clonogenic cells during freezing, storage or thawing; the number of cells required for 50% successful transplantation remained close to unity in both cases. The overall data revealed no evidence of an alteration of the receptivity of the mouse colonies over a period of 13 or 20 years. We attribute this remarkable stability to close supervision of the systems within a single laboratory.
D000818:Animals; D005260:Female; D005615:Freezing; D007942:Leukemia, Experimental; D008223:Lymphoma; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D013997:Time Factors; D014021:Tissue Preservation; D014185:Transplantation, Isogeneic