A radioassay for pyrimidine-5'-nucleotidase activity.
A simple method for the determination of pyrimidine-5'-nucleotidase activity in red cells is described. The radioactive uridine released after incubation with [5-3H]] uridine 5-monophosphate (UMP) is separated on DEAE-cellulose paper and counted. This method does not require preliminary dialysis of the hemolysate and is 50-fold more sensitive than that based on the measurement of the inorganic phosphate released. One patient with pyrimidine-5'-nucleotidase deficiency was detected with this method.
D004912:Erythrocytes / Q000201:enzymology*; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D008722:Methods; D009708:Nucleotidases / Q000097:blood*; D010710:Phosphates / Q000097:blood; D011742:Pyrimidine Nucleotides; D014529:Uridine / Q000378:metabolism; D014542:Uridine Monophosphate / Q000378:metabolism
D006454:Hemoglobins; D010710:Phosphates; D011742:Pyrimidine Nucleotides; D014542:Uridine Monophosphate; D009708:Nucleotidases; D014529:Uridine