Nutritional depletion of the established stationary phase during growth of mouse mammary carcinoma cells in suspension culture.
The stationary phase of cell growth in suspension culture was analyzed with regard to nutritional depletion using FM3A cells originally derived from a spontaneous mammary carcinoma in a C3H mouse. When cells reached the stationary phase at the cell density of 5 X 10(5) cells/ml, cells began to lose the ability to form colonies and viability as determined by the dye exclusion test. When the amount of amino acids and vitamins in the medium decreased, cells reached the stationary phase at a low terminal density. Cells in the spent medium supplemented with amino acids, vitamins, glucose, and serum grew with the same growth rate as in the fresh medium. It is concluded that depletion of nutrients, especially amino acids and glucose, is the reason for the establishment of the stationary phase.
D000601:Amino Acids, Essential / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D014815:Vitamins / Q000378:metabolism
D000601:Amino Acids, Essential; D003470:Culture Media; D014815:Vitamins; D005947:Glucose