[Comparison between cytochemical and gelelectrophoretic demonstration of acid phosphatases from leukocytes (author's transl)].
The cytochemical and gelelectrophoretic demonstration of acid phosphatases with substrates alpha-naphthyl phosphate and naphthol AS-BI phosphate and with couplers fast garnet GBC and hexazotized pararosanilin is described. Cytochemically best results are achieved wit naphthol AS-BI phosphate and pararosanilin, electrophoretically however with alpha-naphthyl phosphate and pararosanilin. This discrepancy ist explained by a different substrate affinity and membrane penetration of substrates.
D000135:Acid Phosphatase / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods; D006651:Histocytochemistry; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification; D007962:Leukocytes / Q000201:enzymology*
D007527:Isoenzymes; D000135:Acid Phosphatase
Vergleich zwischen zytochemischer und gelelektrophoretischer Darstellung der sauren Phosphatasen aus Leukozyten.