title: A radioimmunoassay for 6-keto-prostaglandin F1alpha.
abstract: A radioimmunoassay for 6-keto-prostaglandin F1alpha has been developed. The assay is accurate and sensitive but since the antiserum cross-reacts 5-10% with prostaglandins (PGs) of the E and F series, solvent extraction and thin layer chromatography are required for absolute specificity. The assay has been validated by comparison with a radiochemical assay and by the use of an inhibitor of 6-keto PGF1alpha formation, 15-hydroperoxy arachidonic acid. 6-Keto PGF1alpha was found to have a low cross reaction with antisera directed against PGE2, PGF2alpha and thromboxane B2.
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D003429:Cross Reactions; D007106:Immune Sera; D008861:Microsomes / Q000378:metabolism; D011460:Prostaglandins F / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D011463:Prostaglandins H / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods*; D012756:Sheep; D013929:Thromboxane B2 / Q000032:analysis
chemical_list: D007106:Immune Sera; D011460:Prostaglandins F; D011463:Prostaglandins H; D013929:Thromboxane B2