[Complex of single-stranded phage DNA and protein--a product of bacteriophage fl gene 3. Distribution of gene 3 protein in the DNA molecule].
Upon a 50% isopropanol treatment of phage fl in a 1 M NaCl solution protein A (gene 3 product)--DNA complex is precipitated while protein B (gene 8 product) was still solubilized. After such a treatment the DNA--protein complex containing 10--40% of protein A and less than 0.0025% of protein B was obtained. Evidence was obtained that there was no non-specific rearrangement of protein A during the isolation procedure. The complex was treated with endonuclease R.HAC III, followed by electrophoresis of the  resulted fragments and estimation of the [14C] protein A (labeled with [14C]histidine) throughout the gel. The maximal radioactivity coincided with the DNA bands, being proportional to the DNA content in the respective bands. The data obtained indicate that protein A is iniformly arranged along the DNA molecule.
D055598:Chemical Phenomena; D002621:Chemistry; D003090:Coliphages / Q000235:genetics*; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases; D005814:Genes, Viral; D009690:Nucleic Acid Conformation; D014764:Viral Proteins
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins; D004720:Endonucleases
Kompleks odnonitchatoĭ fagovoĭ DNK s belkom--produktom gena 3 bakteriofage fl. Raspredelenie belka gena 3 na molekule DNK.