[An improved modification of the micro-method according to Niesel and Thews for the measurement of O2-hb binding curves in whole blood and concentrated Hb solutions (author's transl)].
An improved modification of the photometric micro-method according to Niesel and Thews is described, which employs the diffusion principle. The O2-Hb binding curves of whole blood or concentrated Hb solutions (0.1-30 g%) can be measured within 0.5 h. Sample volume is approximately 7 microliter, wavelength is 578 nm. Several reproductions of the curve may be measured with one sample. The reproducibility of P50 is 0.5 Torr. In comparison with the Van Slyke method no systematic error is found.
D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D010100:Oxygen / Q000097:blood*; D010783:Photometry / Q000379:methods*; D013997:Time Factors
D006454:Hemoglobins; D010100:Oxygen
Eine verbesserte Modifikation der Mikromethode nach Niesel und Thews zur Messung von O2-Hb-Bindungskurven in Vollblut und konzentrierten Hb-Lösungen.