title: Lipoamide dehydrogenase in cultured human skin fibroblasts.
abstract: Lipoamide dehydrogenase was identified in cultured skin fibroblasts of normal individuals and patients with Friedreich's ataxia. The optimum conditions for its assay were defined. Data disclosed a normal range of 36--122 mumol/min/mg protein in control fibroblasts and 61--112 mumol/min/mg protein in patients fibroblasts. Numerous precautions should be taken in handling fibroblast cultures for lipoamide dehydrogenase determination.
mesh_terms: D002021:Buffers; D002478:Cells, Cultured; D002498:Centrifugation; D003067:Coenzymes / Q000378:metabolism; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology; D005621:Friedreich Ataxia / Q000201:enzymology; D006358:Hot Temperature; D006801:Humans; D009994:Osmolar Concentration; D011506:Proteins / Q000378:metabolism; D012016:Reference Values; D012867:Skin / Q000201:enzymology*; D013997:Time Factors
chemical_list: D002021:Buffers; D003067:Coenzymes; D011506:Proteins; D008058:Dihydrolipoamide Dehydrogenase