Synthesis of prostaglandin 6-keto F1alpha by cultured aortic smooth muscle cells and stimulation of its formation in a coupled system with platelet lysates.
Lysed aortic smooth muscle cells, when incubated with [14C] arachidonate, synthesized only one radioactive product, which was identified as 6-keto-PGF1alpha. Formation of this product from smooth muscle cell lysates was stimulated when human platelet extracts were added to the system, and further stimulation was observed when imidazole, a selective inhibitor of thromboxane synthesis, was added to this coupled system. These observations indicate that the cyclooxygenase of the smooth muscle cells was rate-limiting, that the prostacyclin synthetase of these cells can utilize endoperoxides produced by platelets, and that blocking of thromboxane synthesis might, under certain conditions, shunt arachidonate metabolism toward prostacyclin formation.
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D001095:Arachidonic Acids / Q000378:metabolism; D001158:Arteries / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism*; D011449:Prostaglandin Endoperoxides / Q000378:metabolism; D011460:Prostaglandins F / Q000096:biosynthesis*; D051381:Rats
D001095:Arachidonic Acids; D011449:Prostaglandin Endoperoxides; D011460:Prostaglandins F