[Removal of surface-active substances from proteins by reversible immobilization on water-insoluble carrier].
Techniques for removing of surface-active substances from cysteine and cystine-containing proteins preliminarily immobilized by thiol-disulfide exchange on water-insoluble carrier, followed by protein transfer into solution, as examplified in sodium dodecyl sulfate, cetyltrimethylammonium bromide and bovine serum albumin are described. This procedure may be used to remove any sort of material non-covalently bound to protein.
D002593:Cetrimonium Compounds; D002846:Chromatography, Affinity / Q000379:methods; D003545:Cysteine; D003553:Cystine; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate
D002593:Cetrimonium Compounds; D011506:Proteins; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D003553:Cystine; D003545:Cysteine
Osvobozhdenie belkov ot poverkhnostio-aktivnykh veshchestv metodom obratimoí immobilizatsii na nerastvorimom nostitele.