Quantitative GLC determination of cis- and trans-isomers of doxepin and desmethyldoxepin.
A GLC method for the simultaneous quantitative determination of the cis- and trans-isomers of doxepin and desmethyldoxepin in human plasma was developed. The method involves the use of a capillary column for efficient separation of the four compounds and the internal standards, amitriptyline and nortriptyline. A high sensitivity is obtained with a nitrogen detector, enabling quantitation of the compounds in plasma of humans treated chronically with doxepin. Confirmation of the identity of the cis- and trans-isomers of doxepin and desmethyldoxepin in biological samples was carried out by selected ion monitoring.
D000328:Adult; D000368:Aged; D002849:Chromatography, Gas; D003640:Dealkylation; D004316:Doxepin / Q000031:analogs & derivatives* / Q000097:blood*; D005260:Female; D006801:Humans; D007536:Isomerism; D008722:Methods
D004316:Doxepin