[Study of the chemical receptor for diphtheria toxin in cell membranes].
Adsorption of diphtheria toxin on immobilized cell membrane preparations in the presence of gangliosides was studied. A mixture of gangliosides completely inhibited the adsorption of diphtheria toxin on the cell membranes, both sensitive (HeLa cells, macrophages) and resistant (L-cells) to its action. Neuraminidase-treated gangliosides were less effective. Immobilized gangliosides adsorbed diphtheria toxin effectively in the presence of a protective colloid and detergent triton X-100. The results obtained suggest gangliosides to be diphtheria toxin receptors.
D000327:Adsorption; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D004167:Diphtheria Toxin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005732:Gangliosides / Q000494:pharmacology; D006168:Guinea Pigs; D006367:HeLa Cells / Q000187:drug effects; D007739:L Cells / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D011955:Receptors, Drug / Q000187:drug effects*
D004167:Diphtheria Toxin; D005732:Gangliosides; D011955:Receptors, Drug
Izuchenie khimicheskogo resteptora dlia difteriĭnogo toksina v kletochnykh membranakh.