Mass fragmentographic determination of docosenoic acid in rapeseed oils.
A highly sensitive and accurate reference method for determination of docosenoic acid (mainly erucic acid, 22:1n-9) in different rapeseed oils is described. A fixed amount of [1-14C] erucic acid methyl ester (about 1 microgram) is added to a fixed amount of oil. After treatment with sodium methoxide/methanol reagent and extraction with hexane, the amount of unlabeled erucic acid is determined from the ratio between the recordings at m/e 320 and m/e 322 obtained after analysis with a combined gas chromatograph-mass spectrometer equipped with an MID (multiple ion detector). The two ions used correspond to the M-32 peak in the mass spectrum of unlabeled and [1-14C] labeled erucic acid methyl ester. The relative standard deviation of the method is about 1.8%. The method was compared with a gas chromatographic method for determination of erucic acid.
D001937:Brassica / Q000032:analysis*; D004883:Erucic Acids / Q000032:analysis*; D004952:Esters / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009821:Oils / Q000032:analysis*
D004883:Erucic Acids; D004952:Esters; D005231:Fatty Acids, Unsaturated; D009821:Oils