The effect of the strongly bound protein fraction on sugar transport in human erythrocyte ghosts.
The protein fraction released from human erythrocyte membranes with 90% acetic acid enhanced the transport of several sugar species when enclosed in erythrocyte ghosts. Both the influx and the efflux of D-glucose were increased so that permeation rather than sugar binding was involved. The permeation increase was selective, being found with D-glucose, D-galactose and D-xylose but not with L-glucose or lactose. The protein-dependent sugar transport was saturable and the incorporation of proteins into the ghost membrane brought Jmax to the level corresponding to intact erythrocytes, leaving Km unchanged.
D001692:Biological Transport; D001693:Biological Transport, Active; D001786:Blood Glucose / Q000378:metabolism; D002241:Carbohydrates / Q000097:blood*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008565:Membrane Proteins / Q000097:blood*
D001786:Blood Glucose; D002241:Carbohydrates; D008565:Membrane Proteins