title: Simultaneous determination of reserpine and hydrochlorothiazide in two-component tablet formulations by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic procedure is presented for the simultaneous determination of reserpine and hydrochlorothiazide in two-component tablet formulations. An aliquot of a tetrahydrofuran extract of the tablet, containing polylythiazide as an internal standard, is chromatographed on a microparticulate silica gel column using a mobile phase of 0.01% (v/v) diethylamine, 5% (v/v) chloroform, and 18% (v/v) 2-propanol in n-hexane. The relative standard deviations are 1.2 and 0.6% for the simultaneous determination of reserpine and hydrochlorothiazide, respectively. Seven commericial tablet formulations were found to contain 92.7--101.0% and 98.3--101.4% of the labeled amounts of reserpine and hydrochlorothiazide, respectively.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D006852:Hydrochlorothiazide / Q000032:analysis*; D008722:Methods; D012110:Reserpine / Q000032:analysis*; D013607:Tablets / Q000032:analysis
chemical_list: D004338:Drug Combinations; D013607:Tablets; D006852:Hydrochlorothiazide; D012110:Reserpine