Estrogen and the relaxant effect of intramural noradrenaline on calcium induced contractures in depolarized rat uterus.
The influence of intramural noradrenaline on calcium induced contractures was studied in isolated preparations of rat uterus. The depolarized (127 mM KCl) myometrium of oophorectomized rats responded with contraction followed by a transient relaxation when exposed to 3 mM calcium. The threshold concentration of calcium, where the transient relaxation began to appear, was between 0.25 and 0.5 mM. Blockade of the beta-adrenoceptors with propranolol or noradrenaline depletion with reserpine completely removed the transient relaxation, indicating that the latter was due to release of intramural noradrenaline. Estrogen treatment abolished the relaxant effect of intramural noradrenaline, whereas progesterone was ineffective in this respect. Preparations from rats in natural estrus responded like estrogenized tissues, and diestrus preparations behaved as uteri of oophorectomized rats without estrogen treatment.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D009126:Muscle Relaxation; D009638:Norepinephrine / Q000494:pharmacology*; D010053:Ovary / Q000502:physiology; D011374:Progesterone / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D012110:Reserpine / Q000494:pharmacology; D014590:Uterine Contraction / Q000187:drug effects*
D004967:Estrogens; D011374:Progesterone; D012110:Reserpine; D011433:Propranolol; D002118:Calcium; D009638:Norepinephrine