Separation of human renal renin and pseudorenin by affinity chromatography on hemoglobin-Sepharose-2B.
Human renal renin (EC 3.4.99.19) and pseudorenin were easily separated in a single step by affinity chromatography on hemoglobin-Sepharose-2B. Renin and pseudorenin were monitored by their actions on crude and partially purified hog protein renin substrates at neutral and acidic pH and on synthetic labelled polymeric renin substrate. Under the conditions employed (0.1 M sodium acetate (pH 3.5)/1 M sodium chloride at 4 degrees C) renin does not bind to the affinity adsorbent while pseudorenin is effectively bound and can be eluted only after raising the pH to 6.5. Pseudorenin-free renin prepared by this method is devoid of proteolytic activity toward hemoglobin. The chromatographic behaviour of renal pseudorenin on hemoglobin-Sepharose-2B is similar to that of cathepsin D.
D002846:Chromatography, Affinity; D010450:Endopeptidases / Q000302:isolation & purification*; D006454:Hemoglobins; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D012685:Sepharose
D006454:Hemoglobins; D012685:Sepharose; D010450:Endopeptidases; C003241:pseudorenin