Inter-batch quality differences in purified human prolactin.
During the past four years, we secured six batches of purified human prolactin for iodination by the same lactoperoxidase technique. Two of these batches gave us more satisfactory results in terms of assay sensitivity and consistency than did the others, regardless of the antiserum used. Gel filtration of 125I-labeled hormone revealed that these two batches were composed of at least 55% "small" prolactin while the other four batches contained from 20 to 68% "big" prolactin. This qualitative difference was the most important contribution to assay discrepancies.
D006801:Humans; D007457:Iodine Radioisotopes; D011388:Prolactin / Q000302:isolation & purification*; D011863:Radioimmunoassay
D007457:Iodine Radioisotopes; D011388:Prolactin