Spectrophotometric determination of vitamin A in oils and fats.
A spectrophotometric method for determining vitamin A based on interaction with 50% trichloroacetic acid solution in dichloromethane was developed. The blue reaction product had a maximum absorption at 620 nm and obeyed Beer's law over the concentration range of 0.5-5.0 microgram retinol/ml solution. The molar absorptivity of the reaction product was 1.58 X 10(5). As much as 100 microgram vitamin D2, and beta-carotene up to 12 times the vitamin A concentration, did not interfere with the determination. The results obtained from the determination of vitamin A in cod liver oil and butter showed excellent agreement with the Carr-Price method, 43.013(d).
D002079:Butter / Q000032:analysis*; D003060:Cod Liver Oil / Q000032:analysis*; D005395:Fish Oils / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods; D014801:Vitamin A / Q000032:analysis*
D005395:Fish Oils; D014801:Vitamin A; D003060:Cod Liver Oil; D002079:Butter