Growth curves and stabilization of fresh vaccine cultures of the Clemson University strain of Pastuerella multocida.
Growth curves at 37 C for the Clemson University strain of Pasteurella multocida were about the same in either brain-heart-infusion broth or in a recently developed broth called "special broth TV". Counts in both broths were usually greater than 1 x 10(9) viable bacteria per ml. With the addition of 5 g of dried skim milk to each 100 ml of broth culture in the stationary phase of the growth curve, only a slight decrease in viable counts occurred over the next 7 to 14 days when held at 4 C. Such stabilization of broth cultures of P. multocida gives added flexibility to the use of fresh cultures as a live oral turkey cholera vaccine.
D001428:Bacterial Vaccines; D003470:Culture Media; D010325:Pasteurella / Q000254:growth & development* / Q000276:immunology; D013696:Temperature
D001428:Bacterial Vaccines; D003470:Culture Media