Inclusion of xylan in a medium for the enumeration of total culturable rumen bacteria.
The influence of the inclusion of xylan in a medium for enumeration of total culturable rumen bacteria was investigated. Maximum colony numbers were obtained on a medium, GCSX-2, which contained 0.033% each glucose and cellobiose and 0.067% each soluble starch and xylan. This medium gave higher colony counts than either medium 98-5 of Bryant and Robinson (J. Dairy Sci. 44:1446-1456, 1961), medium 98-5 of Chung and Hungate (Appl. Environ. Microbiol. 32:649-652, 1976), containing an added lucerne (hemicellulose + cellulose) fiber substrate, or medium GCSX-2 with the added lucerne (hemicellulose + cellulose) fraction. The time of collection of rumen fluid influenced the colony counts on the media containing the lucerne fiber substrate but was without effect on medium GCSX-2.
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D002482:Cellulose; D003470:Culture Media; D004187:Disaccharides; D005947:Glucose; D008297:Male; D011134:Polysaccharides; D012417:Rumen / Q000382:microbiology*; D012756:Sheep; D013213:Starch; D014990:Xylans
D003470:Culture Media; D004187:Disaccharides; D011134:Polysaccharides; D014990:Xylans; D002482:Cellulose; D013213:Starch; D005947:Glucose