Excretion of newly synthesized DNA by tonsil lymphocytes.
The main part of newly synthesized DNA is preferentially released in vitro both by non-stimulated or phytohemagglutinin-stimulated tonsil lymphocytes. Freshly isolated cells excrete DNA faster than phytohemagglutinin-stimulated ones. The acid-precipitable 3H-thymidine lost can be accounted for in the culture medium as labeled, double stranded DNA isolated on hydroxyapatite column.
D002470:Cell Survival; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D014066:Palatine Tonsil / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology
D010835:Phytohemagglutinins; D004247:DNA