Elevated rate of 3H-uridine incorporation in regressing rat ventral prostate.
Ventral prostates from adult Sprague-Dawley rats were removed at intervals during the first 2 weeks postcastration. Incubation of tissue with 3H-uridine and 3H-leucine was performed to determine the incorporation rate of radioactivity into the RNA and protein fractions respectively. Prostatic wet weight and 3H-leucine incorporation rate into prostate protein diminished postcastration, whereas 3H-uridine incorporation rate remained relatively high. The data suggest that RNA synthesis is relatively active in comparison with other parameters during regression.
D000818:Animals; D001284:Atrophy / Q000209:etiology; D002369:Castration; D004247:DNA / Q000378:metabolism; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D008297:Male; D009929:Organ Size; D011467:Prostate / Q000378:metabolism* / Q000473:pathology; D012313:RNA / Q000378:metabolism; D051381:Rats; D014529:Uridine / Q000378:metabolism*
D012313:RNA; D004247:DNA; D007930:Leucine; D014529:Uridine