Preparation and fractionation of goat kappa-casein: analysis of the glycan and peptide components.
Whole goat kappa-casein was prepared by chromatography of whole casein on hydroxyapatite. Chromatography of whole kappa-casein on DEAE-cellulose separated 5 fractions. All of them were sensitive to chymosin. Their amino acid and carbohydrate composition, phosphate content and molecular weight were determined. Galactose, N-acetylgalactosamine, N-acetyl and N-glycolyl neuraminic acids were identified in whole kappa-casein. It appears that goat kappa-casein, like cow, buffalo and ewe kappa-caseins, is composed of several fractions having identical peptide chains and differing in their carbohydrate contents. The main fraction, devoid of carbohydrate, was treated with chymosin. The para-kappa-casein and caseinomacropeptide were isolated. Their amino acid composition and phosphate content were determined.
D000818:Animals; D002364:Caseins / Q000032:analysis* / Q000302:isolation & purification; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006041:Goats / Q000378:metabolism*; D008892:Milk / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*
D002364:Caseins; D010455:Peptides; D011134:Polysaccharides