title: The effects of clofibrate feeding on the metabolism of palmitate and erucate in isolated hepatocytes.
abstract: The metabolism of palmitate and erucate has been investigated in hepatocytes isolated from control rats and from rats fed 0.3% clofibrate. Clofibrate increased the oxidation of [1-14C]palmitate 1.5 to 2-fold while the esterification was decreased. At a high concentration of palmitate (1.5 mM), the total rate of fatty acid metabolism was stimulated. Clofibrate stimulated both the oxidation (3.5 to 5-fold) and the esterfication (1.7-fold) of [14-14C]erucate. Erucate undergoes chain-shortening in isolated liver cells. This chain-shortening was stimulated at least 2-fold by clofibrate feedings. The isolated mitochondrial fraction from clofibrate-fed rats showed an increased capacity for oxidation of short-chain acylcarnitines (including acetylcarnitine), while the oxidation of palmitoyl- and erucoylcarnitine showed little change. It is suggested that erucate is shortened by the recently detected beta-oxidation system of peroxisomes.
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D002994:Clofibrate / Q000494:pharmacology*; D004883:Erucic Acids / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010169:Palmitic Acids / Q000378:metabolism*; D051381:Rats
chemical_list: D004883:Erucic Acids; D005231:Fatty Acids, Unsaturated; D010169:Palmitic Acids; D000217:Acyltransferases; D002994:Clofibrate