Reconstitution of hybrid toxin from Fragment A of diphtheria toxin and a subunit of Wistaria floribunda lectin.
A hybrid protein was prepared in which Fragment A of diphtheria toxin was linked to a monovalent subunit of the lectin from Wistaria floribunda seeds by a disulfide bridge. The hybrid molecule was reconstituted effectively from a reduced mixture of Fragment A and the lectin subunit by oxidation with o-phenanthroline and CuSO4. The monovalent hybrid protein was partially purified and found to be toxic to L cells. The toxicity of the hybrid protein on the cells was blocked by the addition of either N-acetyl-D-galactosamine, which specifically binds to the lectin, or by anti-Fragment A antibody.
D000116:Acetylgalactosamine / Q000494:pharmacology; D000906:Antibodies; D004167:Diphtheria Toxin / Q000633:toxicity; D007739:L Cells / Q000187:drug effects; D037102:Lectins / Q000633:toxicity; D010446:Peptide Fragments / Q000276:immunology; D055503:Protein Multimerization
D000906:Antibodies; D004167:Diphtheria Toxin; D037102:Lectins; D010446:Peptide Fragments; D000116:Acetylgalactosamine