RNA methylation in vaccinia virus-infected chick embryo fibroblasts treated with homologous interferon.
Interferon-pretreatment of vaccinia-infected chick embryo fibroblasts resulted in a greater than 50% decrease in ribose methylation of the penultimate "cap" nucleotide in virus-specific mRNA. However, in contrast to results obtained with cell-free systems, in intact infected cells there was (a) no detectable reduction in methylation of the 5'-ultimate m7G of viral mRNA; (b) a virus specificity of the interferon-induced change in mRNA "CAP"-methylation seems unlikely and (c) analysis of the ribosomal and transfer RNA fractions isolated from interferon-treated and control cells revealed identical patterns of methylated nucleotides. Thus, the interferon-induced change in methylation is specific for mRNA "CAPS".
D002478:Cells, Cultured; D007372:Interferons / Q000494:pharmacology*; D008745:Methylation; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D014615:Vaccinia / Q000378:metabolism*; D014616:Vaccinia virus / Q000378:metabolism
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012367:RNA, Viral; D011061:Poly A; D007372:Interferons; D012343:RNA, Transfer