A simple test for detection of sickle hemoglobin in the neonatal period.
The ease of denaturation by shaking of a solution of oxyhemoglobin S (oxy-Hb S) forms the basis for a simple and reliable test for confirmation or rapid detection of Hb S in children and adults. Comparison of results of this test with those from agar gel electrophoresis in capillary blood samples from 187 black neonates at 2 to 7 days of life showed no overlap of the amounts of hemoglobin precipitated between the group of neonates with Hb S (5.7--12.0%) and that without Hb S (0--4.7%). These results indicate that the shaking test, by virtue of being simple, reliable and inexpensive, may be used to detect and confirm Hb S in large numbers of neonates before their discharge from the nursery.
D000755:Anemia, Sickle Cell / Q000175:diagnosis; D004587:Electrophoresis, Agar Gel; D006451:Hemoglobin, Sickle / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis; D010108:Oxyhemoglobins; D013053:Spectrophotometry
D006451:Hemoglobin, Sickle; D010108:Oxyhemoglobins