Isolation of specific and common antibodies to staphylococcal enterotoxins A and E by affinity chromatography.
Specific and common antibodies to staphylococcal enterotoxins A and E (SEA and SEE) were isolated from anti-SEA and anti-SEE antisera by affinity chromatography. Anti-SEA and anti-SEE antisera were passed through a column with the cross-reacting enterotoxin coupled to a CNBr-activated Sepharose 4B matrix. Specifically bound common antibodies were eluted with NaSCN. The isolated specific antibodies reacted only with the homologous enterotoxin, whereas the common antibodies gave a reaction of identify with both enterotoxins in double gel diffusion plates. The common antibodies had a higher titer against SEE than against SEA. The significance of the isolation of antibodies common to two separate protein molecules is discussed.
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004768:Enterotoxins / Q000276:immunology*; D011817:Rabbits; D013210:Staphylococcus / Q000276:immunology*
D000907:Antibodies, Bacterial; D004768:Enterotoxins