Enzymatic sulphation of bile salts in human liver.
An enzyme catalyzing the transfer of the sulphate group from 3' -phosphoadenosine-5' -phosphosulphate to lithocholate and glycolithocholate is identified in the cytosol of human liver. The rate of sulphation was greatest with unconjugates lithocolate. Km values for lithocholate and glycolithocholate were 2 . 10(-6) and 3.3 . 10(-6) M, respectively. No enzyme activity was found in human kidney cytosol. A simple method for quantitative assay of the enzyme in percutaneous liver biopsy specimens is described.
D001647:Bile Acids and Salts / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D006801:Humans; D008095:Lithocholic Acid / Q000031:analogs & derivatives; D008099:Liver / Q000201:enzymology*; D010724:Phosphoadenosine Phosphosulfate; D013466:Sulfurtransferases / Q000378:metabolism*
D001647:Bile Acids and Salts; D010724:Phosphoadenosine Phosphosulfate; D008095:Lithocholic Acid; D013466:Sulfurtransferases