Radioimmunoassay for hepatitis B surface antigen and antibody using a two-component immunoadsorbent.
A solid-phase radioimmunoassay is described for detection of hepatitis B surface antigen and antibody. The assay uses a two-site (antigen and antibody) immunoadsorbent capable of binding antigen and antibody. Antigen is detected by enhanced binding of [125U]antibody through sandwich formations of antibody--antigens--[125I]antibody on the immunoadsorbent. Antibody presence is revealed by inhibition of [125I]antibody binding.
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex / Q000494:pharmacology*; D001666:Binding Sites, Antibody; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D006801:Humans; D007164:Immunosorbents / Q000494:pharmacology*; D011863:Radioimmunoassay
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007164:Immunosorbents