Application of charge shift electrophoresis to antigenic analysis of mycoplasmic membranes by two-dimensional (crossed) immunoelectrophoresis.
Both the anodic migration and resolution of membrane antigens of Mycoplasma arginini were greatly improved in two-dimensional immunoelectrophoresis by incorporating both a neutral detergent, Triton X-100, and an anionic detergent, sodium deoxycholate, in the agarose support for first phase electrophoresis. The mobility of cytoplasmic antigens was unaffected at concentrations of 0.1% or less of sodium deoxycholate.
D000942:Antigens, Bacterial / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D003840:Deoxycholic Acid; D007122:Immunoelectrophoresis / Q000379:methods*; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods*; D009174:Mycoplasma / Q000276:immunology*; D011092:Polyethylene Glycols
D000942:Antigens, Bacterial; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols