title: A general method for the purification of restriction enzymes.
abstract: An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.
mesh_terms: D001419:Bacteria / Q000201:enzymology; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*
chemical_list: D004262:DNA Restriction Enzymes