Suppression of CFU-s activity by rabbit-anti-mouse brain serum can be overcome by treatment with papain.
The suppression of CFU-s activity which is observed after incubation with rabbit-anti-mouse brain sera (RAMB) can be reversed by incubating the antibody coated cells with papain. Papain treatment does not alter the CFU-s membrane since pre-treatment with the enzyme does not protect CFU-s from the lethal effect of RAMB. This technique should allow the use of fluoresceinated or otherwise labelled RAMB for the selection of CFU-s.
D000818:Animals; D000906:Antibodies; D001921:Brain / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology*; D051379:Mice; D010206:Papain / Q000494:pharmacology*; D011817:Rabbits
D000906:Antibodies; D007106:Immune Sera; D010206:Papain