Graphical refinement of the amino acid molar ratio and of the molecular weights of peptides and proteins on the basis of amino acid analyses.
Corrected analysis results are handled graphically to find the most probable molar composition of polypeptides and proteins and to refine the molecular weights, even if only approximate molecular weights are available. The principle and properties of the graphical representation of idealized analyses are discussed first. Examples are given of the solution for a polypeptide consisting of 25 amino acids and for its not quite precise analysis and for a concrete protein - extracellular alkaline protease from Aspergillus flavus. The general character of the method is discussed.
D000596:Amino Acids / Q000032:analysis*; D008722:Methods; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
D000596:Amino Acids; D010455:Peptides; D011506:Proteins