A soft-agar procedure measuring growth of human colonic carcinomas.
Cell suspensions from 5 human colonic carcinomas were fractionated by velocity sedimentation and plated in soft agar. Cluster formation was restricted to the purest fraction of epithelial cells, as had been determined by immuno- and histochemical criteria. Plating efficiencies for the 5 specimens were 1.0-4.5%. The effects of varying the incubation period and inoculum size upon growth were studied using unseparated cell suspensions from 6 specimens. Clusters were apparent after 3 weeks in culture, and maximum cluster formation was typically seen by 5 weeks. Cluster formation appeared concentration-dependent, and individual specimens varied with respect to the inoculum most conducive to growth. The maximum plating efficiencies for unseparated cells were unseparated cells were 0.4-1.7%.
D000362:Agar; D002452:Cell Count; D002478:Cells, Cultured; D002999:Clone Cells; D003110:Colonic Neoplasms / Q000473:pathology*; D003470:Culture Media; D006801:Humans; D008722:Methods; D013997:Time Factors
D003470:Culture Media; D000362:Agar