Formation of thermally stable derivatives of chlordiazepoxide and its desmethyl metabolite for GLC.
A derivatization procedure is described for the GLC determination of subnanogram amounts of chlordiazepoxide and nanogram amounts of N-desmethylchlordiazepoxide. Treatment with acetic anhydride at elevated temperature eliminates the highly polar and unstable nitrone group of these compounds by rearrangement and acetylation. The mass spectrometric fragmentation pattern of the acetyl derivatives is recorded and interpreted.
D002707:Chlordiazepoxide / Q000032:analysis*; D002849:Chromatography, Gas; D003640:Dealkylation; D004355:Drug Stability; D006358:Hot Temperature; D013058:Mass Spectrometry; D008722:Methods
D002707:Chlordiazepoxide