title: Relationship between trinitrophenol and H-2 antigens on trinitrophenyl-modified spleen cells. III. Quantitative aspects of trinitrophenol binding on cells treated with trinitrobenzene sulfonic acid.
abstract: Splenic lymphoblasts or normal spleen cells were treated with varying concentrations of TNBS in order to assess whether cell membrane H-2 molecules were derivatized with TNP. Cells treated with high concentrations of TNBS had their cell membrane H-2 molecules derivatized and functioned antigenically as inhibitors in a cold target TNP-CML competition assay. In contrast, cells derivatized with lower concentrations of TNBS had a significant proportion of their membrane proteins derivatized with TNP but did not have their H-2 molecules derivatized. These latter cells were unable to block anti-TNP cytotoxic effector cells in the competition assay. When cells were treated with 3H-TNBS, it was observed that TNP couples to cell membrane H-2, Ia and Ig molecules, and an estimate of the number of TNP molecules bound per cell at varying concentrations of TNBS was determined. The data obtained are consistent with there being a requirement for TNP to directly derivatize H-2 molecules on the cell membrane in order to create antigenic determinants that can be recognized by cytotoxic anti-TNP effector cells. As an alternative, there may be a requirement for the presence of a high density of TNP molecules per cell rather than direct H-2 derivatization by TNP in order to account for activity.
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002462:Cell Membrane / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006649:Histocompatibility Antigens; D051379:Mice; D008805:Mice, Inbred A; D009578:Nitrobenzenes / Q000276:immunology* / Q000494:pharmacology*; D011817:Rabbits; D013154:Spleen / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology*; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*
chemical_list: D006649:Histocompatibility Antigens; D009578:Nitrobenzenes; D014301:Trinitrobenzenes; D014302:Trinitrobenzenesulfonic Acid