Changes in responsiveness of preimplantation mouse embryos to serum.
Changes in uptake of radioactive uridine and its incorporation into RNA were determined in preimplantation mouse embryos, from the 2-cell to the blastocyst stage, as a measure of their responsiveness to extracellular conditions. Two media were tested, one contained serum and the other contained bovine serum albumen as a control. An increase in the acid-soluble pool occurred at the 8-cell stage and a marked increase in RNA synthesis occurred at the early blastocyst stage when the embryos were incubated with serum.
D000818:Animals; D003470:Culture Media; D004477:Ectogenesis; D004622:Embryo, Mammalian / Q000378:metabolism*; D051379:Mice; D012313:RNA / Q000096:biosynthesis
D003470:Culture Media; D012313:RNA