title: The binding of d-glucosyl-neoglycoproteins to the hepatic asialoglycoprotein receptor.
abstract: The binding of D-glucosyl-neoglycoproteins and D-galactose-terminated glycoproteins to the hepatic asialoglycoprotein receptor of rabbit liver membranes were characterized and compared. The binding of both types of glycoproteins showed the same dependence on calcium concentration, sensitivity to neuraminidase, and degree of inhibition by various carbohydrate derivatives. These results, along with the observation that the rabbit liver membranes bound both the D-glucosyl- and D-galactosyl-terminated glycoproteins to the same extent, indicated that both types of glycoproteins bound to the same receptor. To confirm this hypothesis, receptors were isolated from rabbit livers by affinity chromatography using D-galactosyl-bovine serum albumin or D-glucosyl-bovine serum albumin immobilized on Sepharose. These receptors were shown to be identical by several chemical and immunological criteria as well as in their ability to bind equal amounts of D-galactosyl- and D-glucosyl-terminated glycoproteins. The conclusion is that the rabbit hepatic asialoglycoprotein receptor cannot discriminate between D-galactosyl and D-glucosyl-terminated glycoproteins and binds both.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002462:Cell Membrane / Q000378:metabolism; D005960:Glucosides / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D009439:Neuraminidase; D011817:Rabbits; D011955:Receptors, Drug / Q000302:isolation & purification / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D005960:Glucosides; D006023:Glycoproteins; D011955:Receptors, Drug; D012795:Sialoglycoproteins; D009439:Neuraminidase