Spectrodensitometry of nanogram quantities of cholesterol.
In studies of hydrolysis and synthesis of cholesterol esters by aortic enzymes, conventional methods (aside from isotope methodology) are too insensitive to measure reaction products when few aortas are used. High-performance thin-layer chromatography coupled with spectrodensitometry of plates charred after spraying with a cupric acetate-phosphoric acid reagent permitted quantitation of 10-ng amounts of cholesterol, cholesterol oleate oleate, and oleic acid. Linear calibration curves were obtained after spectrodensitometry of chromatograms containing 10-200 ng of lipid. The results were verified by radioassay.
D000818:Animals; D001011:Aorta / Q000378:metabolism; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000032:analysis* / Q000096:biosynthesis; D002788:Cholesterol Esters / Q000032:analysis* / Q000096:biosynthesis; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D008297:Male; D008832:Microchemistry; D009829:Oleic Acids; D010179:Pancreas / Q000201:enzymology; D011817:Rabbits; D013053:Spectrophotometry / Q000379:methods; D002787:Sterol Esterase / Q000378:metabolism
D002250:Carbon Radioisotopes; D002788:Cholesterol Esters; D009829:Oleic Acids; D002784:Cholesterol; D002787:Sterol Esterase