title: A set of analytical electrophoresis experiments to predict the results of affinity chromatographic separations: fractionation of allergens from cow's hair and dander.
abstract: Analytical affinity electrophoresis experiments are described as analogues of column and batch affinity experiments. The are based on crossed immunoelectrophoresis with included affinity, here concanavalian A (con A) and con A-Sepharose. Reactions with serum proteins and allergens from cow's hair and dander are described, and a purification scheme for the latter is described.
mesh_terms: D000485:Allergens / Q000302:isolation & purification*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000494:pharmacology; D006023:Glycoproteins; D006197:Hair / Q000276:immunology*; D006801:Humans; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D012685:Sepharose / Q000494:pharmacology
chemical_list: D000485:Allergens; D006023:Glycoproteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D003208:Concanavalin A; D012685:Sepharose