Effect of hormone on human mammary duct in vitro.
Human mammary ducts were maintained in median-term organ culture. DNA synthesis was assessed by the uptake of 3H-thymidine in epithelial cells; labeling indices were counted on autoradiographies. DNA synthesis grossly remained between starting values in the basic conditions without hormones. Insulin (5 microgram/ml) did not stimulate the uptake of the isotope as compared with the controls. Estradiol (0.001 microgram/ml) and progesterone (1 microgram/ml) highly significantly enhanced DNA synthesis; subsequent waves did occur.
D000328:Adult; D001345:Autoradiography; D001940:Breast / Q000166:cytology / Q000378:metabolism*; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology*
D007328:Insulin; D011374:Progesterone; D004958:Estradiol; D004247:DNA