[Investigation of the interaction of carbamazepine or promazine with bovine serum albumin, using a new dialysis device (author's transl)].
The construction and operation of a new dialysis device are described. The unbound fraction of a drug in a buffered solution of albumin can be determined after 10-15 minutes. The usefullness of this device was demonstrated by the determination of the unbound fraction of Carbamazepine or Promazine in buffered solutions of bovine serum albumin. The results agreed with those obtained by control methods (equilibrium dialysis and gel filtration). Furthermore, the binding of Carbamazepine to bovine serum albumin was characterised by determining the overall binding constant (K1), the apparent binding constant (k+), the number of binding sites per molecule of albumin (n), the free reaction energy deltaFdegrees, and the unbound fraction of the drug.
D001665:Binding Sites; D002220:Carbamazepine; D003956:Dialysis; D011395:Promazine; D011485:Protein Binding; D012710:Serum Albumin, Bovine; D013816:Thermodynamics
D012710:Serum Albumin, Bovine; D002220:Carbamazepine; D011395:Promazine
Untersuchung der Wechselwirkung von Carbamazepin und Promazin mit Rinderserumalbumin mit Hilfe einer neuen Dialysevorrichtung.