[Detection of estrogen residues in meat by thin layer chromatography and fluorimetry (author's transl)].
A new method for the qualitative and quantitative determination of six anabolic substances is described. The analytical procedure consists of the following steps: comminution of the meat sample; homogenisation with tetrahydrofurane; liquid-liquid partition between sodium hydroxide solution and petroleum ether/benzene; chromatography of the estrogen containing fraction on Sephadex LH-20; derivatization with Dansyl chloride; and finally thin layer chromatography. The qualitative estimation on the TLC-plate is performed with 366 nm ultra violet light. The quantitative determination is carried out either by fluorimetry after elution of the substances from the plate, or by means of a remission spectral fluorimeter directly on the plate. The detection limit of the qualitative estimation in meat extracts was 5--10 ppb. A minimum concentration of 20--50 ppb is necessary for quantitative determination.
D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D004958:Estradiol / Q000032:analysis; D004967:Estrogens / Q000032:analysis*; D005470:Fluorometry; D008460:Meat / Q000032:analysis*; D008722:Methods; D015029:Zeranol / Q000032:analysis
D004967:Estrogens; D004958:Estradiol; D015029:Zeranol
Nachweis von Ostrogenrückständen in Fleisch durch Dünnschichtchromatographie und Fluorimetrie.