title: Immunization of pigs against experimental Ascaris suum infection by feeding ultraviolet-attenuated eggs.
abstract: Pigs fed Ascaris suum eggs attenuated by short-wave ultraviolet radiation developed a resistance to challenge infections that varied according to the protocols used. Two doses of eggs irradiated at total exposures (ET) of 150 muW-min/cm2 resulted in a reduction of adult worm burden in 3 groups challenged with 50, 100, or 500 eggs by 45, 35, and 49%, respectively. However, these results were not statistically significant (P greater than 0.05). Three other groups were fed eggs as follows: Group 1, 3 doses irridated at ET's of 150, 150 and 150; Group 2, 3 doses irradiated at ET's of 150, 150, 100; and Group 3, 3 doses irradiated at ET's of 150, 100, and 75. All of these regimens provided significant protection: Group 1 (P less than 0.02), Group 2 (P less than 0.05), Group 3 (P less than 0.001). An analysis of the lengths of worms recovered showed that in controls fed varying doses of eggs, worm sizes were inversely related to the number of eggs in the challenge dose. Measurements of worms from vaccinated animals showed that 23 of 116 adult worms recovered were probably survivors of one or more of the vaccinating doses. A ratio of 18 female to 5 male worms indicated that males are more susceptible to ultraviolet radiation than females.
mesh_terms: D000818:Animals; D001196:Ascariasis / Q000517:prevention & control / Q000662:veterinary*; D001200:Ascaris / Q000276:immunology* / Q000528:radiation effects; D005260:Female; D008297:Male; D010063:Ovum / Q000528:radiation effects; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*; D014612:Vaccines
chemical_list: D014612:Vaccines