Isolation and characterization of a plaque-forming lambda bacteriophage carrying a ColE1 plasmid.
A plaque-forming lambdaimm434 bacteriophage carrying the entire genome of colicinogenic factor E1 has been isolated and characterized. This phage, lambdaimm434ColE1, can lysogenize as a stable plasmid within a recombination-deficient Escherichia coli cell that lacks the normal attachment site for lambda phage. Furthermore, it has been found that lambdaimm434ColE1 phage carrying amber mutations in the O and P genes of the lambda genome, i.e., lambdaimm434OamPamColE1, behaves as a plaque-forming phage, and this finding suggests that the ColE1 factor DNA permits replication of the DNA of the plaque-forming phage.
D003086:Bacteriocin Plasmids; D003090:Coliphages / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D005814:Genes, Viral; D008242:Lysogeny; D009154:Mutation; D010957:Plasmids