Development of a procedure for the assay of an experimental steroid drug in dog plasma.
Studies of the estimation of 16alpha-cyano-3beta-cyclopentyloxypregn-5-en-20-one (an experimental drug) in dog plasma are described. Extraction using a salt/solvent pair (ammonium carbonate/ethyl acetate) is followed by a rapid chromatographic procedure employing Lipidex 5000, which affords a substantially purified fraction. After preparation of the t-butyldimethylsilyloxime, quantification of the drug is performed by selected ion monitoring. The [2H9]cyclopentyloxyl analogue is used as an internal standard. In a preliminary experiment, the advantages (in terms of both sensitivity and selectivity) of the use of an open tubular GLC column are demonstrated.
D000818:Animals; D003517:Cyclopentanes / Q000097:blood; D004285:Dogs; D013058:Mass Spectrometry / Q000379:methods; D009570:Nitriles / Q000097:blood; D011283:Pregnenes / Q000097:blood*
D003517:Cyclopentanes; D009570:Nitriles; D011283:Pregnenes