An economical method for the recovery of albumin from outdated packed cells using polyethylene glycol and heat-fractionation.
Outdated packed cells were pooled and the red cells, together with a large fraction of unwanted globulins, were precipitated with polyethylene glycol. The albumin was then precipitated from the supernatant fluid as a crude paste containing about 85% albumin which was further fractionated using standard techniques.
D000418:Albumins / Q000302:isolation & purification*; D005591:Chemical Fractionation; D004912:Erythrocytes; D006454:Hemoglobins; D006358:Hot Temperature; D006801:Humans; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors
D000418:Albumins; D006454:Hemoglobins; D011092:Polyethylene Glycols; D012965:Sodium Chloride