Molecular relationships of degradative plasmids determined by in situ hybridisation of their endonuclease-generated fragments.
Plasmid inter-relationships were studied by hybridisation of a radioactively labelled DNA probe to endonuclease-derived fragmentation patterns of plasmids bound to a nitrocellulose filter. The degradative plasmids SAL and NAH were found to be very closely related, but probably one did not give rise to the other by just a single deletion or insertion. Relationships between SAL and other degradative plasmids are complex; substantial homology was found with TOL and other plasmids encoding toluate dissimilation and significant homology was found with OCT.
D004269:DNA, Bacterial / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000378:metabolism; D008722:Methods; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011549:Pseudomonas / Q000648:ultrastructure
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004720:Endonucleases