[Cultured cell isolates of the smooth muscle layer from the rat duodenum].
A culture obtained from rat duodenal smooth muscle layer is described. The cells were isolated by trypsinization (0.2%), and the medium used for culture was either MEM with glutamine and non-essential AA or RPMI, both containing 10% foetal calf serum. The cell culture contained both smooth muscle cells and fibroplasts in proportions varying with the age of the culture. At day 6, cell differentiation is important. At day 12, when the cells are confluent, the majority of the cells are fibroblasts. Although it is difficult, the transfer of cells is possible at least twice.
D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D004386:Duodenum / Q000166:cytology*; D005347:Fibroblasts; D009130:Muscle, Smooth / Q000166:cytology*; D051381:Rats; D013997:Time Factors
D003470:Culture Media
Culture de cellules isolées de la fraction musculaire lisse de duodénum de rat.