Improved electrophoretic resolution of LDH isoenzymes in agar-agarose mixtures by utilizing its endosmotic properties.
A simple technique is described which gives a better separation of lactate dehydrogenase isoenzymes; by mixing a correct proportion of a gel with a high electroendosmotic property such as agar, and a gel with alow electroendosmotic property such as agarose. The different positions of the application slit in lactate dehydrogenase isoenzyme patterns and the effect on the positioning of the application slit after mixing gels with different electroendosmotic properties are discussed.
D000362:Agar; D004563:Electrochemistry; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D009995:Osmosis
D007527:Isoenzymes; D000362:Agar; D007770:L-Lactate Dehydrogenase