[Molecular parameters of sensitization of tannin-treated erythrocytes with Salmonella d-flagellin].
A study was made of the molecular parameters of sensitization of tannin-treated sheep red blood cells with salmonella d-flagellin, depending on the protein dose, exposure, and temperature of the medium; the role played in this process by physical and chemical mechanisms was ascertained, the latter proving to determine the hemosensitization level. Senstivity of the erythrocytic diagnostic agent to the agglutinating action of the specific serum was maximal with a stable binding by a single red blood cell of about 200 000 flagellin molecules, although the limit of stable binding constituted 340 000 molecules per one red blood cell. On condition of saturation limit of the erythrocyte flagellin should block not over half of the cell surface. The temperature elevation during the diagnostic agent preparation is advantageous in technological respect, since it leads to reduction of flagellin expenditure and increases the sensitivity of the passive hemagglutination tests.
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D001666:Binding Sites, Antibody; D003470:Culture Media; D004912:Erythrocytes / Q000187:drug effects; D005408:Flagellin / Q000276:immunology*; D006386:Hemagglutination Tests; D008297:Male; D008970:Molecular Weight; D012485:Salmonella typhi / Q000276:immunology*; D012756:Sheep; D013634:Tannins / Q000494:pharmacology; D013696:Temperature; D014435:Typhoid Fever / Q000175:diagnosis
D001426:Bacterial Proteins; D003470:Culture Media; D013634:Tannins; D005408:Flagellin
Molekuliarnye parametry sensibilizatsii tanizirovannykh eritrotsitov sal'monelleznym d-flagellinom.