The presence of ribosomal glycoproteins. Agglutination of free and membrane-bound ribosomes from wheat germ by concanavalin A1.
Free and membrane-bound ribosomes prepared from wheat germ and extensively washed with 1% Triton X100-sodium deoxycholate, formed large aggregates in the presence of concanavalin A. The ribosomal aggregates were detected by sucrose density gradient centrifugation and immunoprecipitation. SDS-polyacrylamide gel electrophoresis of ribosomal proteins indicated that four ribosomal glycoproteins (MW about 14,000 to 24,000) were present in both free and membrane-bound ribosomes. However, three glycoproteins (MW about 25,000 to 15,000) appear to be underglycosylated or lacking in free ribosomes.
D002462:Cell Membrane; D003208:Concanavalin A; D006023:Glycoproteins / Q000378:metabolism; D046911:Macromolecular Substances; D008970:Molecular Weight; D037121:Plant Lectins; D010944:Plants; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D014908:Triticum
D006023:Glycoproteins; D046911:Macromolecular Substances; D037121:Plant Lectins; D012269:Ribosomal Proteins; D003208:Concanavalin A