title: Immune reconstitution in the immunodeficient chicken by transfer of embryonic lymphoid cells.
abstract: Embryonic chickens were rendered immunodeficient by in ovo injection of homologous IgM on the 10th embryonic day. The immunodeficient embryos were intravenously given lymphoid cells taken from normal embryonic bursa, spleen or thymus on the 15th embryonic day. Gain of splenic or bursal weight, natural antibodies to sheep red blood cells (SRBC), frequencies of rosette forming cells (RFC) binding to SRBC or dinitrophenyl-SRBC (DNP-SRBC) and of immunoglobulin bearing cells (IBC) in the bursa and the spleen were investigated to assess the effect of transferred cells during the embryonic stage. Transferred bursal and splenic cells showed an ability to restore the frequency of RFC or IBC in the recipients. However, reversion from the immunodeficient state was not observed in the thymic cell transfer. These findings suggested that the cells derived from embryonic bursa and spleen contained stem cells which developed into RFC and also into precursors of IBC.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D002060:Bursa of Fabricius / Q000196:embryology / Q000276:immunology; D002642:Chick Embryo / Q000276:immunology*; D007075:Immunoglobulin M / Q000494:pharmacology; D007165:Immunosuppression Therapy; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000276:immunology; D009929:Organ Size; D013154:Spleen / Q000196:embryology / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014184:Transplantation, Homologous
chemical_list: D007075:Immunoglobulin M