Quantitative analysis of the amino-terminal residues of spectrin by use of the transamination reaction.
The metal ion-catalysed transamination reaction has been examined as a means of quantitative amino-terminal analysis of proteins. Application of this method to the erythrocyte membrane protein, spectrin, showed that this protein contained a single amino-terminal residue per 240,000 daltons. This value supports the hypothesis that spectrin is comprised of two polypeptide chains of approx. 220,000 and 250,000 daltons, respectively.
D000586:Amination; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D005069:Evaluation Studies as Topic; D006801:Humans; D046911:Macromolecular Substances; D008565:Membrane Proteins; D008722:Methods; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D012709:Serum Albumin; D013049:Spectrin
D000596:Amino Acids; D046911:Macromolecular Substances; D008565:Membrane Proteins; D010455:Peptides; D012709:Serum Albumin; D013049:Spectrin