Resolution of desmosterol, cholesterol, and other sterol intermediates by reverse-phase high-pressure liquid chromatography.
A highly efficient technique has been developed for the resolution of several sterols that are intermediates in the biosynthesis of cholesterol and that differ only by one carbon-carbon double bond or by one methyl group. The technique described utilizes reverse-phase high-pressue liquid chromatography on a micronBondapak-C18 column with acetonitrile as eluting solvent. This procedure is capable of measuring the enzymatic conversion of desmosterol to cholesterol. This chromatographic separation can be conducted by reverse-phase high-pressure liquid chromatography in approximately 10 min, whereas other procedures can require several days.
D000818:Animals; D002784:Cholesterol / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003600:Cytosol / Q000378:metabolism; D003684:Dehydrocholesterols / Q000302:isolation & purification; D003897:Desmosterol / Q000302:isolation & purification*; D005260:Female; D007810:Lanosterol / Q000302:isolation & purification; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D013261:Sterols / Q000302:isolation & purification*
D003684:Dehydrocholesterols; D013261:Sterols; D007810:Lanosterol; D003897:Desmosterol; D002784:Cholesterol