Quantitation of mitochondrial injury in cultured rat heart endothelioid cells with nitroblue tetrazolium.
A sensitive method is presented for measurement of changes in the permeability of mitchondria in cultured cells. Rat heart endothelioid cells were used to determine the penetration rate of nitroblue tetrazolium (NitroBT) or other reactants into mitochondria in situ. Nitroblue formazan, produced as a consequence of succinate dehydrogenase activity in the mitochondria, was eluted and measured with a spectrophotometer. Prior injury of cells with hypo-osmolar solutions increased the rate of formazan production. Several methods are described or suggested for the statistical analysis of the data.
D000818:Animals; D002463:Cell Membrane Permeability; D003470:Culture Media; D004847:Epithelial Cells; D005562:Formazans; D008722:Methods; D008929:Mitochondria, Heart / Q000502:physiology*; D009580:Nitroblue Tetrazolium; D009994:Osmolar Concentration; D051381:Rats; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013778:Tetrazolium Salts
D003470:Culture Media; D005562:Formazans; D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium; D013385:Succinate Dehydrogenase