Distribution of a platinum anti-tumour drug in HeLa cells by analytical electron microscopy.
The distribution of Pt in HeLa cell sections after cell treatment with cis-Pt(NH3)2Cl2 dissolved in dimethylsulphoxide was probed by analytical electron microscopy. Primary targets were the nucleolus and the inner side of the nuclear double membrane. Even after solvolysis in dimethylsulphoxide the drug reached similar sites. It is suggested that cell death may be due to Pt inhibition at the initiation sites of DNA synthesis.
D002945:Cisplatin / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D010984:Platinum; D011868:Radioisotopes; D013997:Time Factors
D011868:Radioisotopes; D010984:Platinum; D002945:Cisplatin