Stereobiochemical aspects of warfarin isomers for inhibition of enzymatic alkylation of menaquinone-0 to menaquinone-4 in chick liver.
The inhibition of enzymatic activity by the isomers of warfarin for alkylation of menaquinone-0 to menaquinone-4 has been studied in chick liver. Injection of (6,7--3H2)-menaquinone-0 into chicks resulted in the biosynthesis of menaquinone-4 within 3 h. Microsomes of control chicks contained 78 per cent of the total menaquinone-4. (S) (-)-Warfarin and (R) (+)-warfarin isomers inhibited the biosynthesis of menaquinone-4 of about 67 and 17 per cent respectively. Fractionation of soluble microsomal proteins lead to the the isolation of a menaquinone-4 binding protein.
D000478:Alkylation; D000818:Animals; D002645:Chickens; D003600:Cytosol / Q000378:metabolism; D007536:Isomerism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D014166:Transferases / Q000378:metabolism; D014812:Vitamin K / Q000096:biosynthesis / Q000378:metabolism*; D014859:Warfarin / Q000494:pharmacology*
D014812:Vitamin K; D014859:Warfarin; D014166:Transferases