Molecular heterogeneity of secretory IgA anti-DNP antibodies.
Evidence is presented that demonstrates that the microscale sucrose isoelectric focusing technique can be used to assess the molecular heterogeneity of secretory IgA anti-DNP antibodies. The data indicate that substitution of the DNP group on the bacterial carrier, pneumococcus, can limit the structural complexity of secretory IgA anti-DNP antibodies. The data support the concept that factors governing secretory IgA antibody heterogeneity are analogous to those influencing the heterogeneity of serum IgG antibodies.
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens; D004136:Dinitrobenzenes / Q000276:immunology*; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007525:Isoelectric Focusing; D008968:Molecular Conformation; D009578:Nitrobenzenes / Q000276:immunology*; D011487:Protein Conformation; D011817:Rabbits
D000941:Antigens; D004136:Dinitrobenzenes; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D009578:Nitrobenzenes