Biochemical effects of gum arabic, gum tragacanth, methylcellulose and carboxymethylcellulose-Na in rat heart and liver.
Repeated oral administration of commonly used suspending media, gum arabic, gum tragacanth, methylcellulose, and carboxymethylcellulose-Na to rats caused uncoupling of oxidative phosphorylation in liver and heart mitochondria and partial inhibition of mixed function oxidases of liver endoplasmic reticulum, as measured by 2-biphenylhydroxylation and 4-biphenylhydroxylation. There were considerable differences between the compounds with regard to potency and reversibility of these effects. Only methylcellulose at a concentration of 0.5% did not alter mitochondrial function and mixed function oxidases. It is recommended as suspending medium for the use in pharmacological and toxicological experiments.
D000045:Acacia; D000818:Animals; D001713:Biphenyl Compounds; D002266:Carboxymethylcellulose Sodium / Q000633:toxicity*; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005260:Female; D008747:Methylcellulose / Q000031:analogs & derivatives* / Q000633:toxicity*; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism*; D010592:Pharmaceutic Aids / Q000633:toxicity*; D011134:Polysaccharides / Q000633:toxicity*; D051381:Rats; D013535:Suspensions; D014144:Tragacanth / Q000633:toxicity*
D001713:Biphenyl Compounds; D010592:Pharmaceutic Aids; D011134:Polysaccharides; D013535:Suspensions; D014144:Tragacanth; D008747:Methylcellulose; D010088:Oxidoreductases; D002266:Carboxymethylcellulose Sodium