Enhanced identification of lethal yellow (Ay/Ay) mouse embryos by means of delayed development of four-cell stages.
At present, experimental studies on the primary genetic lesion in Ay/Ay embryos are restricted by the absence of techniques designed to identify Ay homozygotes prior to the onset of secondary defects. This paper describes a methodology to enrich the yields of Ay homozygotes in experimental populations by taking advantage of an early homozygous Ay expression-lagging development in 4-cell embryos.
D000818:Animals; D005260:Female; D005804:Genes, Lethal; D006720:Homozygote; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology* / Q000235:genetics; D011247:Pregnancy