title: Membrane lipid modifications: biosynthesis and identification of phosphatidyl-N-methyl-N-isopropylethanolamine in rat liver microsomes.
abstract: In previous studies on the modification of polar head groups of membrane phospholipids with the unnatural base analog, N-isopropylethanolamine, we reported an unidentified phospholipid in addition to phosphatidyl-N-isopropylethanolamine in the various membrane fractions of rat liver. The structure of this phospholipid has now been identified as phosphatidyl-N-methyl-N-isopropylethanolamine by nuclear magnetic resonance spectroscopy, and by chromatographic and enzymic analysis. In addition, we found that when rats were injected intraperitoneally with the N-methyl-N-isopropylethanolamine, 19% of the liver microsomal phospholipid was phosphatidyl-N-methyl-N-isopropylethanolamine.
mesh_terms: D000818:Animals; D004983:Ethanolamines / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000096:biosynthesis*; D008862:Microsomes, Liver / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis*; D051381:Rats
chemical_list: D004983:Ethanolamines; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; C017628:phosphatidyl-N-methyl-N-isopropylethanolamine