A radio-gas chromatographic method for the determination of 11-oxotestosterone in fish plasma: its use in confirming estimations by radioimmunoassay.
A radio-gas chromatographic method has been devised for the estimation of 11-oxotestosterone (17beta-hydroxyandrost-4-ene-3, 11-dione) in fish plasma samples which provides an independent means of validating the radioimmunoassay described earlier. Estimates of the concentration of 11-oxotestosterone in a sample of male rainbow trout plasma by radio-gas chromatography using peak height and peak weight measurements were 6.9 microgram/100 ml and 7.4 microgram/100 ml respectively, in good agreement with that of 7.1 microgram/100 ml determined by radioimmunoassay.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D005399:Fishes / Q000097:blood*; D008297:Male; D011863:Radioimmunoassay; D013739:Testosterone / Q000031:analogs & derivatives* / Q000097:blood; D014316:Tritium; D014337:Trout / Q000097:blood
D014316:Tritium; D013739:Testosterone; C003600:11-ketotestosterone