Collection of granulocytes for transfusion. The effect of collection methods on cell enzyme release.
When granulocytes are collected by either discontinuous-flow centrifugation or filtration leukapheresis, lysozyme is released. More lysozyme is released during the filtration procedure than during the centrifugation procedure. A small amount of red cell lysis occurs during the centrifugation but not the filtration procedure. A rise in lactate dehydrogenase levels consonant with the amount of hemolysis is observed. These findings suggest that granulocytes collected by either filtration or discontinuous-flow centrifugation undergo degranulation but not lysis sufficient to cause cytoplasmic enzyme release during the donation.
D001803:Blood Transfusion; D002498:Centrifugation; D005374:Filtration; D006098:Granulocytes / Q000637:transplantation*; D006454:Hemoglobins; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007937:Leukapheresis; D009113:Muramidase / Q000097:blood; D013997:Time Factors
D006454:Hemoglobins; D007770:L-Lactate Dehydrogenase; D009113:Muramidase