title: Effect of various hexoses on sperm capacitation and penetration of rat eggs in vitro.
abstract: Fertilization of rat eggs in vitro could not be achieved when epididymal spermatozoa were preincubated and eggs in clots incubated in a chemically defined medium without D-glucose. Very high proportions (84-100%) of eggs examined were undergoing fertilization when 2.78-8.34 mM-D-glucose were included in the medium. The substitution of D-fructose or D-galactose for D-glucose resulted in very poor penetration rates (0-4%), but D-mannose was effective for fertilization (59-99% penetration). Incubations for sperm capacitation and egg fertilization in different media containing the various hexoses showed that rat epididymal spermatozoa could be partly capacitated without hexose, that D-glucose, D-mannose or D-galactose but not D-fructose is effective for sperm capacitation and that only D-glucose and D-mannose supported penetration of eggs in vitro.
mesh_terms: D000818:Animals; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects; D005632:Fructose / Q000494:pharmacology; D005690:Galactose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008358:Mannose / Q000494:pharmacology; D051381:Rats; D013075:Sperm Capacitation / Q000187:drug effects*; D013084:Sperm-Ovum Interactions / Q000187:drug effects
chemical_list: D005632:Fructose; D005947:Glucose; D008358:Mannose; D005690:Galactose