A method for the recovery of DNA from agarose gels.
We describe a quick and versatile method for the isolation of DNA from agarose gels. The DNA is electrophoresed into a trough containing hydroxyapatite, where it is bound. The hydroxyapatite is taken out and the DNA eluted with phosphate buffer. By putting the hydroxyapatite on a small column of Sephadex G50, elution and subsequent removal of phosphate can be performed in one step. The DNA recovered can be used equally well in enzymatic incubations as DNA not purified through agarose gel electrophoresis. Several applications of this technique are described.
D003090:Coliphages; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005914:Globins / Q000235:genetics; D006882:Hydroxyapatites; D008970:Molecular Weight
D004270:DNA, Circular; D004279:DNA, Viral; D006882:Hydroxyapatites; D005914:Globins; D004247:DNA; D004262:DNA Restriction Enzymes