[Optimization of immobilization conditions for acid proteinase from Aspergillus awamori].
To optimize the immobilization conditions for acid proteinase from Aspergillus awamori by covalent binding through glutaraldehyde, experiments were carried out using the Box-Wilson method. The optimization process was assessed on the basis of absolute activity A, coefficient of activity retention gamma and their product A gamma. The following conditions can be recommended: glutaraldehyde concentration 50--60 mg/g, enzyme concentration not less than 40 mg/g, time of glutaraldehyde treatment 2--2.5 hrs, immobilization time 2 hrs, pH about 4.0, and temperature 35--40 degrees C. Under these conditions A=220--230 U/g, gamma = 23--24% Agamma = 5,000--6,000.
D001230:Aspergillus / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D010447:Peptide Hydrolases / Q000378:metabolism*
D004800:Enzymes, Immobilized; D010447:Peptide Hydrolases
Optimizatsiia usloviĭ immobilizatsii kisloĭ proteinazy iz Aspergillus awamori.