Soluble endoribonuclease activity from vaccinia virus: specific cleavage of virion-associated high-molecular-weight RNA.
A soluble endoribonuclease activity was extracted from purified vaccinia virus cores by treatment with sodium-deoxycholate and dithiothreitol. The soluble enzyme readily cleaved purified virion-associated high-molecular-weight RNA to limit-sized fragments sedimenting at 8 to 12S. Purified virion-released 8 to 12S polyadenylated mRNA was not degraded by the enzyme extract. The soluble endoribonuclease did not require the presence of ribonucleoside triphosphates for activity.
D004720:Endonucleases / Q000378:metabolism*; D008970:Molecular Weight; D012367:RNA, Viral / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D012995:Solubility; D013379:Substrate Specificity; D014616:Vaccinia virus / Q000201:enzymology*
D012367:RNA, Viral; D004720:Endonucleases; D012260:Ribonucleases