Use of dithiothreitol for improved visibility of the F body in human Y-bearing spermatozoa.
Dithiothreitol was used to decondense the nuclear chromatin before staining human spermatozoa with quinacrine for the F body present in Y-bearing spermatozoa. In unseparated fractions and those enriched in X- or Y-bearing spermatozoa, the number of spermatozoa with F bodies increased by 2-9% after dithiothreitol treatment.
D002469:Cell Separation / Q000379:methods*; D004229:Dithiothreitol; D005144:F Factor; D006801:Humans; D008297:Male; D012730:Sex Chromosomes; D013094:Spermatozoa / Q000648:ultrastructure*; D014998:Y Chromosome
D004229:Dithiothreitol