The in vitro reassembly of rough endoplasmic reticulum: ribosome binding capacity.
Rough endoplasmic reticulum membranes were dissolved in 1% deoxycholate and the deoxycholate was then dialysed out for five days. Well-defined bilayer vesicles were formed only if the dialysis was performed at room temperature for the first six hours. The vesicles were separated into a pelleted fraction (Fraction 1) and a fluffy layer (Fraction II) by centrifugation. As measured by amino acid incorporation ability, Fraction II bound polysomes, while Fraction I did not. When smooth endoplasmic reticulum was assembled, it was found that Fraction II so derived had a polysome binding capacity which was more sensitive to increased KCl concentrations (25 mM - 100 mM KCl) and that it bound significantly more monosomes than the corresponding fraction derived from rough membranes. The SDS-polyacrylamide polypeptide patterns of the various fractions were compared.
D001665:Binding Sites; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D008566:Membranes / Q000378:metabolism; D008567:Membranes, Artificial; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D012270:Ribosomes / Q000378:metabolism*
D008567:Membranes, Artificial; D010455:Peptides; D011189:Potassium Chloride