Measurement of the differential melting profile of a promoter-containing fragment of T7 DNA by means of a microspectrophotometer.
A double-beam microspectrophotometer with a 5 microliter cell has been used to study denaturation of DNA in an aqueous solution. This instrument enables measurement of high-resolution differential melting profiles simultaneously at several wavelengths with 0.5 to 1 microgram of DNA. Therefore it becomes possible to study nucleic acids which are difficult to obtain in large amounts. The techniques have been employed to measure the differential melting profiles of T7 DNA and of a fragment of this DNA 1000 base pairs long which contains the four early promoters.
D001483:Base Sequence; D001665:Binding Sites; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005809:Genes, Regulator; D005814:Genes, Viral; D006358:Hot Temperature; D008904:Miniaturization; D009691:Nucleic Acid Denaturation; D013053:Spectrophotometry / Q000295:instrumentation
D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases