Evaluation of the discriminative power of lipidophoresis by discriminant analysis using a simple quantitative method of agarose lipidophoresis.
A simple, reproducible and quantitative method for separation of lipoproteins in agarose is presented. The validity of the results is shown by comparison with gravimetric analysis of fractions separated by ultracentrifugation in a discontinuous NaCl-KBr gradient. Results for a large group of normals and of patients with various lipid disorders are presented, and discriminant analysis is used to evaluate objectively the potential of lipidophoresis in the classification of hyperlipoproteinemias. It is shown that quantitative lipidophoresis gives as much information as the determination of cholesterol and triglycerides, for classification purposes.
D000328:Adult; D001797:Blood Protein Electrophoresis / Q000379:methods; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008875:Middle Aged; D014461:Ultracentrifugation
D008074:Lipoproteins