Idiotypic analysis of anti-GAT antibodies. I. Presence of common idiotypic specificities in both responder and nonresponder mice.
The synthetic terpolymer of L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) induced anti-GAT antibodies in D1.LP mice. Some of these antibodies shared common idiotypic specificities. The interaction between idiotypic antibody and anti-idiotypic antiserum was specifically inhibited by GAT and the closely related polymer of L-glutamic acid and L-tyrosine (GT) but not by the copolymer of L-glutamic acid and L-alanine (GA). The binding of labeled GAT by the anti-GAT antibodies was also specifically inhibited by the anti-idiotypic antiserum. These results indicated a close association of idiotypic determinants with the antibody-combining sites. Furthermore, such active site-related common idiotypic specificities were found in individual sera of both responder mice (D1.LP) and nonresponder mice (DBA/1) immunized with GAT and GAT-MBSA, respectively, indicating that both responder and nonresponder mice carry B cells with common clonotypes.
D000409:Alanine / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D004306:Dose-Response Relationship, Immunologic; D005971:Glutamates / Q000276:immunology*; D006168:Guinea Pigs; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes / Q000302:isolation & purification; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011817:Rabbits; D014443:Tyrosine / Q000276:immunology*
D005971:Glutamates; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes; D014443:Tyrosine; D000409:Alanine