Fc receptors induced by herpes simplex virus. I. Biologic and biochemical properties.
Radioiodinated normal rabbit IgG was used to detect Fc receptors on the surface of HeLa S3-2 cells infected with herpes simplex virus (HSV). Unlike the Fc receptors present on most leukocytes, these virus-induced Fc receptors were found to be sensitivite to trypsin at concentrations of enzyme as low as 0.1 mg/ml. Treatment of infected cells with neuraminidase enhanced the binding of IgG. Yet the HSV-induced Fc receptor(s) is probably a glycoprotein because its synthesis was inhibited by 2-deoxy-D-glucose at concentrations inhibiting glycoproteins but not total proteins. Binding of radioiodinated IgG to Fc receptors on infected HeLa S3-2 cells was unaffected by F(ab')2 fragments prepared from antisera against uninfected HeLa S3-2 cells or against human beta2-microglobulin. By contrast, anti-HSV F(ab')2 or Fab' fragments decreased binding of radioiodinated IgG to HSV-infected cells by 85%, and binding of radioiodinated Fc was also inhibited by anti-HSV Fab'.
D000818:Animals; D001667:Binding, Competitive; D003847:Deoxyglucose / Q000494:pharmacology; D006367:HeLa Cells / Q000276:immunology; D006561:Herpes Simplex / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D009439:Neuraminidase / Q000494:pharmacology; D011817:Rabbits; D014357:Trypsin / Q000494:pharmacology
D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D003847:Deoxyglucose; D009439:Neuraminidase; D014357:Trypsin