[Determination of serum iron; a comparison of two methods: atomic absorption and bathophenanthroline without deproteinisation (author's transl)].
Two methods for the assay of serum iron are compared; determination by atomic absorption after deproteinisation, and spectrophotometric determination after liberation of the iron by a detergent, reduction with dithionite, and chelation with bathophenanthroline disulfonate, without deproteinisation.


RESULTS
Regression: y = 0.996 x --0.41 (s: atomic absorption; y: bathophenanthroline method; mumol/1 Fe; r = 0.986. Precision (CV) for a series of identical samples with 14.3 mumol/1: 2.0% for atomic absorption and 1.3% for bathophenanthroline.
D001798:Blood Proteins; D006801:Humans; D007202:Indicators and Reagents; D007501:Iron / Q000097:blood*; D010618:Phenanthrolines; D013054:Spectrophotometry, Atomic / Q000379:methods
D001798:Blood Proteins; D007202:Indicators and Reagents; D010618:Phenanthrolines; C006686:bathophenanthroline; D007501:Iron
Serumeisenbestimmung; Methodenvergleich Atomabsorption/Bathophenanthrolin ohne Enteiweissung.