title: Nuclear magnetic resonance studies in liposomes: effects of steroids on lecithin fatty acyl chain mobility.
abstract: The effects of fourteen sterols on the NMR spectra of liposomes derived from egg yolk phosphatidylcholines were studied by continuous-wave and Fourier-transform measurements at 60 MHz. Sterols were compared for their ability to broaden the acyl methylene resonances of phosphatidylcholine, when incorporated into liposomes at 25% molar ratio. The ratio of the phosphatidylcholine peake heights (acyl methylene: choline N-methyl) was used as a criterion of the relative condensing activity for the different sterols. This ratio was inversely proportional to the molar volume of the incorporated sterol, as measured by the parachor of the compound. Small sterols had little condensing effect, and the larger sterols such as cholesterol and ergosterol had maximum condensing effects. The study confirmed the importance of the sterol side-chain at C-17 as a requirement for sterol-phospholipid interaction.
mesh_terms: D005947:Glucose / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010539:Permeability; D010713:Phosphatidylcholines; D013256:Steroids; D013261:Sterols; D013329:Structure-Activity Relationship
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D013256:Steroids; D013261:Sterols; D005947:Glucose