title: Derivation and maintenance of gnotobiotic dogs.
abstract: Revised techniques for derivation and maintenance of gnotobiotic dogs for research purposes were outlined. Gravid uteri were removed from pregnant beagle bitches and passed through a germicidal trap into a flexible plastic isolation unit. Pups were raised on a milk replacement diet and weaned to solid food by 4 weeks of age.
mesh_terms: D000821:Animal Feed; D000818:Animals; D004285:Dogs / Q000254:growth & development / Q000601:surgery; D005260:Female; D005856:Germ-Free Life; D006799:Housing, Animal; D008722:Methods; D011247:Pregnancy; D014599:Uterus / Q000601:surgery