The preparation and analysis of myelin from small quantities of central nervous tissue: regional studies of the quaking mouse.
Myelin of considerable purity may be isolated from small (minimum 1 mg wet weight) samples of central nervous tissue, using a 4-step centrifugation procedure. The separation of myelin proteins by micro-linear gradient polyacrylamide gel electrophoresis yields similar results to those obtained by macro-scale (homogeneous) gel systems. These techniques have been employed for a preliminary study of the regional composition of myelin fractions from the Quaking mouse.
D000818:Animals; D001923:Brain Chemistry; D002498:Centrifugation / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D051379:Mice; D008821:Mice, Quaking / Q000502:physiology*; D008832:Microchemistry / Q000379:methods; D009185:Myelin Proteins / Q000032:analysis; D009186:Myelin Sheath / Q000032:analysis*; D011510:Proteolipids / Q000032:analysis; D013116:Spinal Cord / Q000032:analysis*
D009185:Myelin Proteins; D011510:Proteolipids