Inhibition of mitochondrial DNA synthesis by aflatoxin B1 and dimethylnitrosamine.
The acute and subchronic effects of aflatoxin B1 and dimethylnitrosamine (DMN) on in vitro incorporation of thymidine-3H into DNA (mt-DNA) from mouse liver mitochondria were studied after single injection or after one month or three months of weekly injection. Both carcinogens induced 50% decreases in mt-DNA synthesis acutely, with a 50% decrease in synthesis of high molecular weight mt-DNA. Subchronic administration of aflatoxin B1 inhibited mt-DNA synthesis by 54% with elution profiles similar to the controls. DMN showed only a 29% inhibition of mt-DNA synthesis at one month increasing to 55% at three months.
D000348:Aflatoxins / Q000494:pharmacology*; D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D003864:Depression, Chemical; D004128:Dimethylnitrosamine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D009602:Nitrosamines / Q000494:pharmacology*; D013997:Time Factors
D000348:Aflatoxins; D009602:Nitrosamines; D004247:DNA; D004128:Dimethylnitrosamine