A granulocyte test for detection of cattle heterozygous for mannosidosis.
alpha-Mannosidase and beta-N-acetylglucosaminidase activities were found to differ between lymphocytes and granulocytes isolated from bovine blood. Activities of both enzymes in granulocyte preparations were found to be related to the eosinophil content of the preparations. A procedure is described that allows definition of the mannosidosis genotype of adult cattle by giving consideration to the influence of eosinophil content of granulocyte preparations upon the activity of alpha-mannosidase relative to that of beta-N-acetylglucosaminidase.
D000118:Acetylglucosaminidase / Q000097:blood; D000818:Animals; D002239:Carbohydrate Metabolism, Inborn Errors / Q000235:genetics / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D006580:Genetic Carrier Screening; D006027:Glycosides / Q000378:metabolism*; D006098:Granulocytes / Q000201:enzymology*; D007962:Leukocytes / Q000201:enzymology; D008361:Mannosidases / Q000097:blood; D008362:Mannosides / Q000378:metabolism*; D008722:Methods; D013997:Time Factors
D006027:Glycosides; D008362:Mannosides; D008361:Mannosidases; D000118:Acetylglucosaminidase