Degradation of phenylalanine:pyruvate transaminase after glucagon treatment.
Using a single-isotope and immune precipitation technique the half-life (t 1/2) of hepatic phenylalanine:pyruvate transaminase (aminotransferase, EC 2.6.1.--, Number not yet assigned) from glucagon-treated rats was determined to be 2.8 days, similar to that of the control rats (t 1/2 = 3.3 days). The half-life of rat liver total soluble proteins also remained unchanged after glucagon treatment (t 1/2 = 2.7 days in glucagon-treated rats; t 1/2 = 2.8 days in normal). Thus, glucagon has no effect on the degradation of phenylalanine:pyruvate transaminase. Furthermore, the degradation rates are similar for both the holoenzyme and the apoenzyme of phenylalanine:pyruvate transaminase.
D000818:Animals; D001051:Apoenzymes; D004790:Enzyme Induction; D005934:Glucagon / Q000494:pharmacology*; D006207:Half-Life; D008099:Liver / Q000201:enzymology*; D008297:Male; D010649:Phenylalanine; D011233:Precipitin Tests; D011773:Pyruvates; D051381:Rats; D012995:Solubility; D000637:Transaminases / Q000378:metabolism*
D001051:Apoenzymes; D011773:Pyruvates; D010649:Phenylalanine; D005934:Glucagon; D000637:Transaminases