title: Stability of polyadenylated RNA in differentiating myogenic cells.
abstract: Three independent methods of measurement showed that cytoplasmic polyadenylated RNA from the differentiating myogenic cell line L8 consists of two main populations with regard to stability, one with a half-life of less than 4 h and the other with a half-life of 17--54 h. Similar results were obtained in the presence and absence of actinomycin D. During the fusion of mononucleated myoblasts into multinucleated fibers, there was an increase in both the steady-state pool of the more stable polyadenylated RNA and the proportion of stable polyadenylated RNA synthesized in pulse labelling.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002645:Chickens; D004247:DNA / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D004355:Drug Stability; D007700:Kinetics; D008433:Mathematics; D008970:Molecular Weight; D009124:Muscle Proteins / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*
chemical_list: D009124:Muscle Proteins; D003609:Dactinomycin; D011061:Poly A; D012313:RNA; D004247:DNA