Demonstration of a delay time during aggregation of diluted solutions of deoxyhemoglobin S and hemoglobin CHarlem in concentrated phosphate buffer.
Diluted solutions (less than 100 mg/dl) of deoxygenated sickle hemoglobin (Hb S) and Hb CHarlem were found to aggregate with a clear demonstration of a delay time when dissolved in concentrated phosphate buffer. The delay time could be shortened by increasing the concentration of either hemoglobin or phosphate. Upon cooling or bubbling with carbon monoxide, the aggregates melted quickly. The logarithmic plot of reciprocal delay time versus hemoglobin or phosphate concentration shows a linear line with a slope of 2.7 for hemoglobin and 44 for phosphate. This suggests that diluted solutions of Hb S aggregate by the nucleation mechanism which is similar to that reported in the gelation of concentrated Hb S solution.
D002021:Buffers; D006444:Hemoglobin C; D006451:Hemoglobin, Sickle; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D010710:Phosphates
D002021:Buffers; D006451:Hemoglobin, Sickle; D046911:Macromolecular Substances; D010710:Phosphates; D006444:Hemoglobin C