Topographic studies of glycoproteins of intact synaptosomes from rat brain cortex.
Glycoproteins in the external surface of intact synaptosomes from rat brain cortex have been studied by oxidation of exposed galactose and galactosamine groups by galactose oxidase followed by reduction with labeled sodium borohydride. Purified synaptosomes were labeled, disrupted by osmotic shock, and the particulate components were fractionated on diatrizoate to give four synaptosomal membrane fractions (A to D) and a mitochondrial pellet (E). Fractions A and B represent highly purified synaptosomal plasma membranes. After separation of their polypeptides by electrophoresis, 4/5 of the label was present in two bands: one about 72 000 and the other between 7800 and 3200 daltons. Seven other bands were labeled to various degrees: 160 000, 96 000, 53 000, 39 000, 34 000, 23 000 and 16 000 daltons. With isolated membranes (which incorporate 5--6 times more label) 4/5 of label was present in polypeptides in three ranges: 160 000--96 000, 70 000--40 000 and 7800--3200. The number of polypeptides that can be labeled by treatment of isolated membranes is very large. In comparison, glycoproteins whose topographical distribution permits interaction with large molecules at the synpatic surface are very limited. It is further suggested that the external synaptosome membrane involves a relatively tight network of interacting molecules that cannot be readily penetrated by large molecules.
D000818:Animals; D001894:Borohydrides; D001931:Brain Mapping; D002540:Cerebral Cortex / Q000032:analysis*; D005692:Galactose Oxidase; D006023:Glycoproteins / Q000032:analysis*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D051381:Rats; D013574:Synaptosomes / Q000032:analysis*
D001894:Borohydrides; D006023:Glycoproteins; D008565:Membrane Proteins; D005692:Galactose Oxidase