title: Estimation of Michaelis--Menten constants in microsomal enzyme systems by the direct linear plot and least squares regression.
abstract: 1. Data from in vitro liver microsomal metabolism of ethylmorphine aniline and benzpyrene in male rats and male and female quokkas (Setonix Brachyurus) were fitted to the Michaelis--Menten equation by Wilkinson's non-linear regression procedure and Eisenthal and Cornish-Bowden's direct linear plot. 2. Ethylmorphine and aniline metabolism in the male quokka were non-ideal, but in all other instances there were no differences in the performance of the two methods.
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism; D005036:Ethylmorphine / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D007614:Macropodidae; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats; D012044:Regression Analysis; D013045:Species Specificity; D013223:Statistics as Topic
chemical_list: D000814:Aniline Compounds; D001580:Benzopyrenes; D004364:Pharmaceutical Preparations; D005036:Ethylmorphine