Enzyme determination and RAST inhibition assays for orchard grass (Dactylis glomerata): a comparison of commericial pollen extracts.
Pollens release enzymes when they are dropped into water. Orchard Grass extracts were tested by two methods. The Api-Zym System determined 19 enzymes and colorimetric amounts of Leucine Aminopeptidases, Acid phosphatase and trypsin. These were compared with RAST inhibition assays. A good correlation was shown between the techniques. Thus the enzyme dosage may be useful for the standardization of pollen extracts.
D004306:Dose-Response Relationship, Immunologic; D010936:Plant Extracts / Q000276:immunology*; D006109:Poaceae / Q000201:enzymology* / Q000276:immunology; D011058:Pollen / Q000201:enzymology; D011852:Radioallergosorbent Test
D010936:Plant Extracts