Selective potentiation of hyperthermic killing of hypoxic cells by 5-thio-D-glucose.
The modification of cell lethality at elevated temperatures by 5-thio-D-glucose, the nearest analog of D-glucose was studied with the use of HeLa cells in the presence or absence of oxygen. When the cells were exposed to temperatures ranging from 40.5-42 degrees for 0.5 to 4 hr in the presence of 5-thio-D-glucose (5 mM), there was a selective potentiation of hyperthermic killing of hypoxic cells. No enhanced killing of oxic cells by 5-thio-D-glucose was seen at these temperatures. These results indicate that glucose metabolism may be involved in influencing the heat sensitivity of hypoxic cells. The low toxicity of 5-thio-D-glucose and its specificity to hypoxic cells suggest that it may be a useful adjuvant to hyperthermic therapy.
D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D005947:Glucose / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006358:Hot Temperature; D006979:Hyperthermia, Induced; D000860:Hypoxia; D009369:Neoplasms / Q000628:therapy; D010100:Oxygen
D005947:Glucose; D010100:Oxygen