title: Physiological and pharmacological studies on isolated human bronchial preparations.
abstract: The contractile response of human bronchial smooth muscle preparations obtained after death (necrobronchi) from 15 patients was studied in vitro. Reproduceable responses sufficient for calculating dose-response curves, could be obtained up to 30 h after death. A number of smooth muscle stimulants were investigated: 1) carbacholine, 2) acetylcholine, 3) histamine and 4) electrical field stimulation. In addition to these bronchial smooth muscle relaxants such as adrenaline, salbutamol and terbutaline were also studied. These agents showed a dose-dependent relaxation in preparations previously treated with carbacholine. It can be concluded that in vitro testing of smooth muscle preparations obtained from human cadavers is possible.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000368:Aged; D000420:Albuterol / Q000494:pharmacology; D001980:Bronchi / Q000187:drug effects / Q000502:physiology*; D002217:Carbachol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D004837:Epinephrine / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D008722:Methods; D008875:Middle Aged; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D013726:Terbutaline / Q000494:pharmacology
chemical_list: D006632:Histamine; D002217:Carbachol; D013726:Terbutaline; D000109:Acetylcholine; D000420:Albuterol; D004837:Epinephrine