A modified wire-loop method for quantitative electron microscopic radioautography.
A simplified method of emulsion coating and development for quantitative electron microscopic radioautography without any special instrument in introduced. Seven grids carrying ultrathin sections are attached to a glass block with double-coated Scotch tape. They are coated with a wire-loop, 2.5 cm in diameter. Ten glass blocks are then stuck on a slide, exposed, developed, fixed and stained simultaneously. Employing this method, the emulsion coating and the following procedures are easily accomplished under an identical condition.
D001345:Autoradiography / Q000295:instrumentation / Q000379:methods*; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods*