Mode of binding of pyridoxal 5'-phosphate in rat liver ornithine aminotransferase.
1. Pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate were effective for the association of apo-form II of ornithine aminotransferase [EC 2.6.1.13]; whereas other B6 derivatives, such as pyridoxal, pyridoxamine, pyridoxine and pyridoxine 5'-phosphate, had no effect on form II of this apoenzyme. 2. The pyridoxal 5'-phosphate contents of the native enzyme, and reconstituted forms I and II were determined by two different methods to be 1.5 moles, 2.5 moles and 3.3 moles of pyridoxal 5'-phosphate/mole of enzyme, respectively.
D000818:Animals; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D009943:Organophosphorus Compounds / Q000494:pharmacology; D009953:Ornithine-Oxo-Acid Transaminase / Q000378:metabolism*; D011485:Protein Binding; D011730:Pyridoxal / Q000494:pharmacology; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D011733:Pyridoxamine / Q000031:analogs & derivatives / Q000494:pharmacology; D011736:Pyridoxine / Q000494:pharmacology; D051381:Rats; D000637:Transaminases / Q000378:metabolism*
D046911:Macromolecular Substances; D009943:Organophosphorus Compounds; D011730:Pyridoxal; D011732:Pyridoxal Phosphate; D011733:Pyridoxamine; D000637:Transaminases; D009953:Ornithine-Oxo-Acid Transaminase; D011736:Pyridoxine