Studies on the tissue specificity of the high-mobility-group non-histone chromosomal proteins from calf.
The high-mobility-group (HMG) non-histone chromosomal proteins from calf thymus, liver, spleen and kidney were extracted, and fractionated by CM-Sephadex chromatography and trichloroacetic acid precipitation. The isolated proteins HMG 1, HMG 2 and HMG 17 from the tissues were compared by polyacrylamide-gel electrophoresis, isoelectric focusing and amino acid analysis. The results show that the three proteins are very similar in the tissues studied, implying a lack of tissue specificity.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009928:Organ Specificity; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
D000596:Amino Acids; D002868:Chromosomal Proteins, Non-Histone