title: Effect of medium composition on the growth and asparaginase production of Vibrio succinogenes.
abstract: Asparaginase synthesis by Vibrio succinogenes is induced by ammonium ions. Synthesis occurs throughout exponential phase, and in early stationary phase asparaginase accounts for about 5% of the total soluble protein. The organism grows best when fumarate is provided as the terminal electron acceptor of the formate-oxidizing cytochrome system. Yeast extract or enzyme-hydrolyzed proteins are effective nutrient sources. In an ammonium formate-sodium fumarate medium, where maximum growth and asparaginase synthesis occurs, the total enzyme yield (international units per liter of culture) is about one-tenth that obtainable with a good asparaginase-producing strain of Escherichia coli. The energetic inefficiency of V. succinogenes appears to cause a low yield of cells and therefore low total enzyme yield. However, the levels of asparaginase accumulated within cells raise questions about the organism's protein synthesizing system.
mesh_terms: D001215:Asparaginase / Q000096:biosynthesis*; D003470:Culture Media; D005561:Formates / Q000378:metabolism; D005650:Fumarates / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D014733:Vibrio / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D005561:Formates; D005650:Fumarates; D000644:Quaternary Ammonium Compounds; D001215:Asparaginase