Synthesis of a mammalian parvovirus in Brij-58-lysed cells.
An in vitro system was developed, prepared by lysis of parvovirus LuIII-infected cells with Brij-58. In this subcellular system, DNA of viral genome size and synthesized and subsequently packaged into particles with physicochemical properties of mature LuIII virions.
D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis*; D009024:Morphogenesis; D010321:Parvoviridae / Q000254:growth & development / Q000378:metabolism*; D013347:Subcellular Fractions; D013501:Surface-Active Agents / Q000494:pharmacology*; D014779:Virus Replication
D004279:DNA, Viral; D013501:Surface-Active Agents