[Potentiometric determination of hepatitis B surface antigen in biological fluids (author's transl)].
The present report describes a sensitive and reliable method for potentiometric determination of hepatitis B surface antigen in biological fluids, as an application of enzyme-linked immunoassay technique by a "sandwich" procedure. Specific antibodies are immobilized on a gelatine membrane which covers the sensor of an iodide-sensitive electrode. After immersion of the modified electrode in a dilute solution of antigen, the enzymatic activity is evaluated in a solution of peroxidase-labelled antibodies, in the presence of substrate and iodides. The electrode potential is a function of antigen concentration in the solution. This new procedure should find its application in the determination of substances present in very low concentration in biological fluids.
D000906:Antibodies; D004566:Electrodes; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D007454:Iodides / Q000032:analysis; D008027:Light; D008567:Membranes, Artificial; D008722:Methods; D010544:Peroxidases / Q000378:metabolism; D011199:Potentiometry; D013053:Spectrophotometry
D000906:Antibodies; D006514:Hepatitis B Surface Antigens; D007454:Iodides; D008567:Membranes, Artificial; D010544:Peroxidases
Détermination potentiométrique de l'antigène de surface de l'hépatite B dans les liquides biologiques.