A set of analytical electrophoresis experiments to predict the results of affinity chromatographic separations: fractionation of allergens from cow's hair and dander.
Analytical affinity electrophoresis experiments are described as analogues of column and batch affinity experiments. The are based on crossed immunoelectrophoresis with included affinity, here concanavalian A (con A) and con A-Sepharose. Reactions with serum proteins and allergens from cow's hair and dander are described, and a purification scheme for the latter is described.
D000485:Allergens / Q000302:isolation & purification*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000494:pharmacology; D006023:Glycoproteins; D006197:Hair / Q000276:immunology*; D006801:Humans; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D012685:Sepharose / Q000494:pharmacology
D000485:Allergens; D006023:Glycoproteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D003208:Concanavalin A; D012685:Sepharose