[Possible influence of plasma factors on lymphocyte blastigenesis and E rosette forming in rheumatoid arthritis (author's transl)].
The lymphocytes blastigenesis to PHA in culture with autologous or normal plasma, the formation of E rosettes and the presence of immunoglob-lins on the lymphocytes surface were evaluated in 51 patients with chronic primary polyarthritis. While the percentage of T and B cells was almost normal (52.1 +/- 2.1% and 18.1 +/- 2.1%, N.V. 55.6 +/- 2.0% and 16.2 +/- 0.8%), the blastigenesis was significantly reduced in cultures with a 25% autologous plasma (5399 +/- 709 cpm compared to 13,182 +/- 920 cpm of the normal standard) and returned towards normal values when it was replaced by the plasma of a normal subject (12,189 +/- 1081 cpm). The rheumatoid plasma was capable of reducing the blastigenesis in 11 normal subjects (7395 +/- 750 cpm). The preincubation for 90' of the lymphocytes of 14 controls in rheumatoid or autologous plasma induced a significant reduction in the formation of spontaneous rosettes (40.8 +/- 1.4 % and 56.2 +/- 1.7%). These results suggest that the reduced function of the T lymphocytes in this disease is due to plasma factors.
D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology* / Q000473:pathology; D002908:Chronic Disease; D005260:Female; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012397:Rosette Formation; D013268:Stimulation, Chemical
D037102:Lectins; D011947:Receptors, Antigen, B-Cell
Uber den möglichen Einfluss plasmatischer Faktoren auf die lymphozytäre Stimulierbarkeit und E-Rosetten-Bildung bei primär chronischer Polyarthritis.