title: [Effect of insulin on germination and ionic exchange in Raphanus sativus (author's transl)].
abstract: Hypoglycemic sulfamide BZ-55 activates or inhibits germination of Raphanus sativus, depending upon the dosis. Since this drug acts upon the glycemia by increasing the secretion and action of insulin, the influence of this hormone on germination and ionic changes (Na+-K+) between seeds and culture medium, were studied. Seeds were incubated during 72 h with different concentrations of insulin in 10 ml deionized water or in 10 ml 18 mM K+ (KCl) solutions at 37 degrees C in vapor saturated atmosphere. A solution of 0.125 IU insulin/ml in water increases the germination to 110% whereas 0.175 IU insulin/ml inhibits it to 40% against controls. Further increases in insulin concentration always inhibit germination. Similar results have been obtained with K+ containing media. Germination rate changes in a small concentration range suggest that insulin might affect an enzymatic activity in the seed.
mesh_terms: D001786:Blood Glucose / Q000494:pharmacology*; D002271:Carbutamide / Q000494:pharmacology*; D003470:Culture Media; D004789:Enzyme Activation; D007328:Insulin / Q000494:pharmacology*; D007474:Ion Exchange; D009994:Osmolar Concentration; D011188:Potassium / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000201:enzymology*; D012964:Sodium / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D003470:Culture Media; D007328:Insulin; D012964:Sodium; D002271:Carbutamide; D011188:Potassium
vernacular_title: Efectos de la insulina sobre la germinación e intercambio iónico del Raphanus sativus.