Affinity chromatographic demonstration of a thrombin binding protein from the platelet plasma membrane.
Human platelet plasma membrane glycoprotein I with an apparent molecular weight of approximately 150,000 has been shown to be one of the proteins retained by thrombin immobilized on Sepharose 4B. The retained glycoprotein has been recovered by sodium dodecyl sulfate elution and characterized by SDS polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol.
D001792:Blood Platelets / Q000032:analysis* / Q000648:ultrastructure; D001798:Blood Proteins / Q000032:analysis*; D002352:Carrier Proteins / Q000097:blood*; D002462:Cell Membrane / Q000032:analysis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013917:Thrombin / Q000378:metabolism*
D001798:Blood Proteins; D002352:Carrier Proteins; D013917:Thrombin