Studies on the microcytotoxicity test. III. Comparison of  [75Se]selenomethionine with [3H]proline, Na2, 51CrO4 and [125I]iododeoxyuridine for pre-labelling target cells in long-term cytotoxicity tests.
Four intracellular radioisotope labels, [3H]proline, Na2 51CrO4, [75Se]selenomethionine and [125I]iododeoxyuridine, were evaluated for use in a pre-labelling long-term microcytotoxicity assay for cell-mediated immunity. Adherent rat tumour cells established in tissue culture were used as targets and the basic variables studied were labelling efficiency, toxicity and spontaneous release rates. [125I]Iododeoxyuridine was found unsuitable on account of its high toxicity and correspondingly high spontaneous release rate, and Na2 51CrO4 for its toxicity and low labelling efficiency. Of the two other radiolabels, [75Se]selenomethionine had the advantage over [3H]proline of higher labelling efficiency (especially in Ham's F10 medium), lower toxicity, and being a gamma-emitter. Furthermore, released 75Se was shown to be non-reutilisable and its retention by target cells provided an accurate measure of cell survival in an alloimmune system. Methods of calculating the results of pre-labelling cytotoxicity tests based on the total radioactivity in target cells at the beginning of the assay were found to be invalid.
D000818:Animals; D002840:Chromates / Q000378:metabolism*; D003601:Cytotoxicity Tests, Immunologic; D007065:Idoxuridine / Q000378:metabolism* / Q000633:toxicity; D007553:Isotope Labeling; D011392:Proline / Q000378:metabolism* / Q000633:toxicity; D051381:Rats; D012643:Selenium / Q000378:metabolism*; D012645:Selenomethionine / Q000378:metabolism* / Q000633:toxicity; D013997:Time Factors
D002840:Chromates; D012645:Selenomethionine; D011392:Proline; D012643:Selenium; D007065:Idoxuridine