Monolayer immune complex (MIC) fractionation of Fc receptor bearing human spontaneous killer cells.
A simple high recovery method is described for depletion and enrichment of Fc receptor bearing human spontaneous killer cells. The technique is called monolayer immune complex (MIC) fractionation and is based on the fact that Fc receptor positive cells will bind to immune complexes bound to plastic surface and that bound cells can be partially recovered by protein A elution.
D000818:Animals; D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D007694:Killer Cells, Natural / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000494:pharmacology; D013997:Time Factors
D000936:Antigen-Antibody Complex; D007141:Immunoglobulin Fc Fragments; D013205:Staphylococcal Protein A