A rapid biosynthetic method for the preparation of radioactive phosphatidyl-CMP(CDP-diacylglycerol) of high specific activity.
A procedrue is described for the preparation of [32P]phosphatidyl-CMP(CDP-diacylglycerol) from rat pineal glands incubated with [32P]orthophosphate and DL-propranolol. The product is 95% radiopure and a high specific acitivity. The yield of liponucleotide is 0.4--0.9 micron Ci/mCi of [32P]orthophosphate in the medium. The same method can also be used for the biosynthesis and purification of [3H]phosphatidyl-CMP when [3H]cytidine is the precursor.
D000818:Animals; D003562:Cytidine / Q000378:metabolism; D003567:Cytidine Diphosphate Diglycerides / Q000096:biosynthesis* / Q000302:isolation & purification; D007553:Isotope Labeling; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D010761:Phosphorus Radioisotopes; D010870:Pineal Gland / Q000378:metabolism; D051381:Rats
D003567:Cytidine Diphosphate Diglycerides; D009702:Nucleoside Diphosphate Sugars; D010761:Phosphorus Radioisotopes; D003562:Cytidine