Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.
A reliable method for the separation of free and esterified cholesterol in adipocyte extracts is described. The procedure uses Sephadex LH-20, a lipophilic dextran gel, with a solvent system of chloroform-hexane 55:45 (v/v). Interference by excess triglyceride, such as that encountered in adipocyte total lipid extracts, was not observed, and overall recovery of both sterols exceeded 98%.
D000273:Adipose Tissue / Q000032:analysis*; D000375:Aging; D000818:Animals; D002784:Cholesterol / Q000032:analysis*; D002788:Cholesterol Esters / Q000032:analysis; D002850:Chromatography, Gel; D008297:Male; D051381:Rats; D014280:Triglycerides / Q000032:analysis
D002788:Cholesterol Esters; D014280:Triglycerides; D002784:Cholesterol