[Determination of a primary structure of abnormal D-like hemoglobin isolated from a blood donor].
Abnormal hemoglobin with contents 40% was isolated from the blood of a donor. It was purified, its chains were separated, and the defect was found to locate at beta-chain. Abnormal betaT13 peptide was found under the separation of tryptic hydrolysate. Data on its amino acid composition, electrophoretic mobility as well as attempts to determine its N-terminal amino acid and N-terminal sequence made possible to identify the location and the character of the amino acid substitution in the abnormal hemoglobin. The first case of the presence of hemoglobin D Penjab alpha2beta2(121Glu leads to Gln) among the Russian population is described.
D000595:Amino Acid Sequence; D001782:Blood Donors; D002852:Chromatography, Ion Exchange; D004586:Electrophoresis; D006455:Hemoglobins, Abnormal / Q000302:isolation & purification*; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis
D006455:Hemoglobins, Abnormal; D010446:Peptide Fragments
Ustanovlenie pervichnoĭ struktury anomal'nogo D-podobnogo gemoglobina, vyiavlennogo v krovi donora.