Purification of milk-clotting protease from Bacillus mesentericus strain 76.
A two-step procedure for the isolation of pure enzyme from Bacillus mesentericus strain 76 is developed. Ion-exchange chromatography on CM-Sephadex-C50 is used as a second step after precipitation from ethanol. The pure enzyme preparation, obtained after gel filtration on Sephadex-G25 of the ion-exchange chromatographically separated product, possess the highest specific activity achieved. The homogeneity of the final preparation is proved by determination of the N-terminal amino acid (Arg) and by SDS-PAGDE (single disc).
D000818:Animals; D001407:Bacillus / Q000201:enzymology*; D002417:Cattle; D003611:Dairy Products; D008892:Milk; D010447:Peptide Hydrolases / Q000302:isolation & purification*
D010447:Peptide Hydrolases