Chicken lymphocyte stimulation by mitogens: a microassay with whole-blood cultures.
A micromethod is described that uses whole blood for studying mitogen stimulation of chicken lymphocytes. Procedures critical for obtaining a stimulatory response up to 200 times that of unstimulated control cultures include: 1) use of no more than 10 microliter of whole blood per culture; 2) incubation at 41 C for 72 hours in 0.2 ml of medium RPMI 1640 containing 100 microgram of stock phytohemagglutinin-P and 3) pulse labeling for 8 hours with 5-[125I] iodo-2'-deoxyruidine. The method is easy, rapid, and suitable for sequential studies of pathogenesis of disease in poultry.
D000818:Animals; D001769:Blood; D002645:Chickens / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D013154:Spleen / Q000276:immunology; D013696:Temperature
D003470:Culture Media; D037102:Lectins; D003208:Concanavalin A