title: Fine structure of reactive cells in injured nervous tissue labeled with 3H-thymidine injected before injury.
abstract: To examine the fine structure of blood mononuclear cells in injured nervous tissue, mice were given repeated injections of 3H-thymidine with the last injection at least 16 hours before injury. Under ether anesthesia the animals either were given a stab wound to the spinal cord or had their left hypoglossal nerve transected. The animals were killed at 2, 4, 8, or 16 days after injury. Tissue sections containing the spinal cord wound or both hypoglossal nuclei were prepared for electron microscopic radioautography, and all labeled cells were photographed. About half the labeled cells in the injured spinal cords and almost all the labeled cells in the nuclei of the injured hypoglossal nerves had nuclei with dark staining peripheral heterochromatin, dark cytoplasm with long cisternae of granular endoplasmic reticulum, and other ultrastructural features characteristic of the cells usually identified as microglia. The remaining labeled cells in the injured spinal cords were macrophages, fibroblasts, cells with pale nuclei, some of which contained cytoplasmic filaments, and vascular cells. Since uninjured nervous tissue has extremely few labeled cells and since 3H-thymidine should be available for only a short time following injection, most of the labeled cells in this experiment should be derived from blood mononuclear cells. However, the possibility is discussed that some or all of the labeled cells may be intrinsic cells proliferating in response to the injury and labeled through reutilization of labeled DNA precursor material.
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D007002:Hypoglossal Nerve / Q000648:ultrastructure; D061228:Hypoglossal Nerve Injuries; D007962:Leukocytes / Q000648:ultrastructure*; D051379:Mice; D008938:Mitosis; D009000:Monocytes / Q000648:ultrastructure; D009416:Nerve Regeneration; D009457:Neuroglia / Q000648:ultrastructure; D010587:Phagocytosis; D013119:Spinal Cord Injuries / Q000473:pathology*; D013936:Thymidine; D013997:Time Factors; D014316:Tritium; D014945:Wound Healing
chemical_list: D014316:Tritium; D013936:Thymidine