title: An insect cell line persistently infected with a baculovirus-like particle.
abstract: A persistent infection by a baculovirus-like particle was found in the established lepidopteran (Heliothis zea) cell line, IMC-HZ-1. The virus caused CPE in less than 1% of the IMC-HZ-1 cells, as measured by phase-contrast and electron microscopy. Transmission tests showed that four lepidopteran cell lines were susceptible to the persistent virus (designated as IMC-HZ-I-NOV). In inoculated TN-368 cell cultures, 90--100% infection was achieved. The ultrastructure and development of IMC-HZ-I-NOV in cell cultures were similar to known baculoviruses, and it is probable that this persistent virus is a member of the family Baculoviridae. Two lepidopterous species (Estigmene acrea and H. zea) inoculated with IMC-HZ-1-NOV by intrahemocoelic injection and/or per os feeding of larvae were not susceptible.
mesh_terms: D002460:Cell Line; D002467:Cell Nucleus / Q000382:microbiology / Q000648:ultrastructure; D003588:Cytopathogenic Effect, Viral; D007304:Insect Viruses / Q000254:growth & development* / Q000648:ultrastructure; D007814:Larva / Q000382:microbiology; D007915:Lepidoptera / Q000382:microbiology