Substrate activation of cell adhesion factor as a prerequisite for cell attachment.
Cell Adhesion Factor, complexed to insoluble collagen-coated tissue culture dishes, is required for the attachment of fibroblasts to this substrate. In solution, the factor has no demonstrable affinity for cells in suspension following trypsin-EDTA removal of cells from monolayer. Cell surface receptors for the factor are present during the assay period since cells allowed to recover for 1 h at 37 degrees C, 4 degrees C or in the presence of 10(-6) M cycloheximide show exactly the same kinetics of adhesion as control cells. It is demonstrated that Cell Adhesion Factor acquires affinity for the cell surface only following its binding to collagen.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002472:Cell Transformation, Viral; D003094:Collagen / Q000378:metabolism; D006224:Cricetinae; D005347:Fibroblasts / Q000378:metabolism; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D014357:Trypsin
D006023:Glycoproteins; D003094:Collagen; D014357:Trypsin