title: The effects of sonication, freezing and lyophilization on JMV leukosis strain.
abstract: JMV lymphoblastic leukemic cells were subjected to sonication, followed by freezing and lyophilization in an attempt to learn the tolerance of JMV cells to these treatments. Sonication experiments indicated that a high percentage of cell breakage (greater than 89%) is necessary for any decrease in lethality to be observed. Freezing experiments involving a wide range of cryoprotectors demonstrated 2M glycerol to be the best for JMV preservation. Subsequent freeze-drying of sonicated, frozen JMV preparations, of high titer, consistently resulted in all loss of lethality.
mesh_terms: D000818:Animals; D002470:Cell Survival; D002474:Cell-Free System; D002645:Chickens; D003451:Cryoprotective Agents / Q000494:pharmacology; D005612:Freeze Drying; D005615:Freezing; D008380:Marek Disease / Q000382:microbiology*; D013010:Sonication
chemical_list: D003451:Cryoprotective Agents