Zonal centrifuge purification of human rabies vaccine obtained on bovine fetal kidney cells. Biological results.
An inactivated human rabies vaccine prepared on bovine fetal kidney cells is concentrated and purified by zonal centrifugation. The peak of rabies particles is monitored by hemagglutination. Immunogenicity of the purified particles was evaluated by titration of specific antibodies from vaccinated animals. Protective activity of the vaccine was assayed on guinea pigs challenged with street rabies. Biological results were compared with those obtained with other tissue culture vaccines.
D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral / Q000032:analysis; D002501:Centrifugation, Zonal; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006801:Humans; D051379:Mice; D009500:Neutralization Tests; D011819:Rabies Vaccines / Q000276:immunology / Q000302:isolation & purification*; D014613:Vaccines, Attenuated
D000956:Antigens, Viral; D011819:Rabies Vaccines; D014613:Vaccines, Attenuated