title: The effect of protein deficiency on some rat liver lipid metabolic enzymes and CoA.
abstract: Two groups of male Wistar rats were fed normal (i.e., 18%) and protein-free diets, respectively, for 7 weeks. In vivo incorporation of [1-14C] acetate into palmitic, stearic, oleic, and arachidonic acids by the liver was reduced in the protein-deficient rats. In vitro incubation of liver microsomes with labeled palmitate or linoleate revealed no change in the specific activities of chain elongating or desaturating enzymes. Protein deficiency resulted in a decrease in specific activity of short chain acyl-CoA synthetase and in total CoA, accompanied by the virtual disappearance of acyl-CoA and an increase in free CoA. Furthermore, there was less microsomal fatty acid synthetase and mitochondrial beta-hydroxybutyrate dehydrogenase activity. These results are discussed in relation to fatty acid synthesis and the changes in liver fatty acid composition.
mesh_terms: D000818:Animals; D003065:Coenzyme A / Q000378:metabolism*; D003066:Coenzyme A Ligases / Q000378:metabolism*; D064429:Fatty Acid Synthases / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D011488:Protein Deficiency / Q000378:metabolism*; D051381:Rats
chemical_list: D005230:Fatty Acids, Nonesterified; D006884:Hydroxybutyrate Dehydrogenase; D064429:Fatty Acid Synthases; D003066:Coenzyme A Ligases; D003065:Coenzyme A