Isolation of amiloride-resistant clones from dog kidney epithelial cells.
The diuretic drug amiloride has been used to select variants from the dog kidney cell line MDCK which are partially resistant to killing by the drug. Evidence is presented suggesting that resistance to killing by amiloride is conferred by decreased uptake of amiloride, which reduces the intracellular drug concentration below the toxic level.
D000584:Amiloride / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002450:Cell Communication; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D004848:Epithelium / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D009154:Mutation; D011719:Pyrazines / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism
D011719:Pyrazines; D000584:Amiloride; D012964:Sodium