Effect of papaverine on Ca2+ uptake by a mitochondrial fraction isolated from rat uterine smooth muscle.
A mitochondrial fraction was isolated from rat uterine smooth muscle. The effect of papaverine (3 X 10(-6), 10(-5), 3 X 10(-5), 10(-4) M) on Ca2+ accumulation by the fraction was studied. The effect of papaverine was changed by using different substrates for Ca2+ accumulation. Papaverine inhibited the Ca2+ accumulation supported by glutamate plus ATP concentration-dependently. The Ca2+ accumulation supported by ATP alone was also inhibited. On the other hand, the Ca2+ accumulation supported by succinate and ATP was increased by papaverine. The effect was not concentration-dependent; papaverine (3 X 10(-5) M) was the most effective concentration for the effect with a shorter incubation (2 min).
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004347:Drug Interactions; D005260:Female; D005971:Glutamates / Q000494:pharmacology; D066298:In Vitro Techniques; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism*; D009130:Muscle, Smooth / Q000378:metabolism* / Q000648:ultrastructure; D010208:Papaverine / Q000494:pharmacology*; D051381:Rats; D013386:Succinates / Q000494:pharmacology; D013997:Time Factors; D014599:Uterus / Q000378:metabolism* / Q000648:ultrastructure
D005971:Glutamates; D013386:Succinates; D000255:Adenosine Triphosphate; D010208:Papaverine; D002118:Calcium