Identification of placental alkaline phosphatase in human sera using polyacrylamide gel electrophoresis.
Polyacrylamide gradient gel electrophoresis may be used to separate placental alkaline phosphatase from other types of serum alkaline phosphatase. This method, of which specificity is demonstrated by the thermostability and immunological reaction of the placental form, is sensitive enough to detect an activity of 0.02 mU in a sample, and seems suitable for use in investigating placental isoenzyme in serum.
D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood*; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008175:Lung Neoplasms / Q000201:enzymology; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
D007527:Isoenzymes; D000469:Alkaline Phosphatase