title: Metabolic effects of some electrofluorimetric dyes.
abstract: The effect of five electrofluorimetric dyes on mitochondrial metabolism was examined to determine their suitability for mitochondrial studies and other biological uses. The dyes merocyanine 540, 8-anilino-1-naphthalene sulfonic acid and bis(1,3-dibutyl barbituric acid-(5))-pentamethane oxonol were found to be inhibitors of the respiratory chain. However, the first two exerted their effect only at high concentrations. 3.3'-Dihexyl-2,2'-oxacarbocyanine was found to act as an uncoupler. 3,3'-Dipropyl-thiocarbocyanine inhibited beta-hydroxybutyrate respiration while dissociating succinate supported respiration from the phosphorylation of ADP. Merocyanine 540 and 8-anilino-1-naphthalene sulfonic acid may be the best suited for studies of membrane potentials in mitochondria since their effect on metabolism is negligible
mesh_terms: D000818:Animals; D004330:Drosophila; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006885:Hydroxybutyrates / Q000378:metabolism; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009930:Organic Chemicals; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats
chemical_list: D005456:Fluorescent Dyes; D006885:Hydroxybutyrates; D009930:Organic Chemicals; C017283:electrofluorimetric dyes