The quantitative determination of 5-S-cysteinyldopa and dopa in normal serum and in serum from patients with malignant melanoma by means of high-pressure liquid chromatography.
A method is described for quantitative determination of 5-S-cysteinyldopa and Dopa in serum involving high-pressure liquid chromatographic analysis (HPLC) and electrochemical detection. The chromatographic system allows estimation of injected amounts corresponding to 25 pg of 5-S-cysteinyldopa and Dopa. In normal subjects the mean serum 5-S-cysteinyldopa concentration was 2.8 ng/ml (range 0.4--12 ng/ml) and the mean serum Dopa 6.3 ng/ml (range 4--10 ng/ml). Patients with melanoma metastases showed increased serum concentrations of 5-S-cysteinyldopa.
D002851:Chromatography, High Pressure Liquid; D003548:Cysteinyldopa / Q000097:blood*; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000097:blood*; D004355:Drug Stability; D004563:Electrochemistry; D006801:Humans; D008545:Melanoma / Q000097:blood*; D008722:Methods
D003548:Cysteinyldopa; D004295:Dihydroxyphenylalanine