[Examinations of aminoacids in aqueous humour after intravitreous injection of ascorbic acid (author's transl)].
The ascorbic acid concentration of the vitreous of rabbits was experimentally increased to about 20 times the normal value. 24 h after the intravitreal injection of ascorbic acid 13 out of 16 free amino acids were decreased, only Glutamicacid, Glycine and Alanine were increased. 48 h after the injection 13 amino acids were lowered. On the fourth day 8 amino acids were decreased in relation to normal values. After 4 weeks the concentrations of 5 amino acids were raised further compared to the normal values, but 10 amino acids were concentrated lower than the normal values. Also Glu-acid, Gly and Ala had fallen down to the range of the normal values. The total amino acids include the free amino acids and the amino acids, which we find after 6 h hydrolysis. On the first day 11 amino acids showed elevations, 7 amino acids were decreased. After 4 weeks Taurin, Lysine and Histidin were higher than the normal values, 15 amino acids were lower. In single cases the differences were statistically significant. We suppose, that the changes of amino acids are caused by the ascorbic acid or their derivates. There is an anterior drainage after intravitreal injection. We come to the conclusion, that there is a reversible disturbance of the diffusion and active transport of amino acids by ascorbic acid.
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000378:metabolism*; D007267:Injections; D011817:Rabbits; D014822:Vitreous Body
D001205:Ascorbic Acid
Aminosäureuntersuchungen im Kammerwasser nach intravitrealer Injektion von Ascorbinsäure.