A convenient method for the determination of non-specific binding in commercially available solid phase iodine labeled radioimmunoassay kits.
57Co-cyanocobalamin is substituted for iodine labeled antigen in three commercially available solid phase radioimmunoassay kits to evaluate the extent of non-specific binding by determination of the supernatant and residual fraction of unbound ligand and thus obtain a value for corrected binding throughout the range of the calibrated standard curve. In the present state of technology correction appears to have a trifling effect on the overall accuracy of the assay; however, the method as described allows the laboratorian to confirm for himself when testing a new product.
D003037:Cobalt Radioisotopes; D004077:Digoxin / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay / Q000379:methods*; D011933:Reagent Kits, Diagnostic
D003037:Cobalt Radioisotopes; D007457:Iodine Radioisotopes; D011933:Reagent Kits, Diagnostic; D004077:Digoxin; D006854:Hydrocortisone