Effect of corticosteroids on committed lymphocytes.
Human peripheral blood lymphocytes were cultured for 48 hr with concanavalin A. The amount of [3H]thymidine incorporated during the last 4 hr of culture, as well as the percentage of rosettes of activated lymphocytes generated, were assayed at the 48th hr. Adding 0.1 M alpha-methyl-D-mannoside (MAM) at progressively later times after the initiation of culture caused progressively less suppression because of the commitment phenomenon. This suppression was not exceeded by the addition of 10(-4) M preparations of corticosteroids and was statistically the same as that induced by a combination of both corticosteroids and MAM. The addition of PGE2 alone and in combination with methylprednisolone also failed to affect [3H]thymidine incorporation by committed lymphocytes.
D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000276:immunology; D008766:Methylmannosides / Q000494:pharmacology; D008775:Methylprednisolone / Q000494:pharmacology; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology; D012397:Rosette Formation
D000305:Adrenal Cortex Hormones; D008766:Methylmannosides; D011459:Prostaglandins E, Synthetic; D003208:Concanavalin A; D008775:Methylprednisolone