A method for the analysis of protein turnover characteristics. Indirect estimation of rates of protein degradation.
A method for the calculation of rate constants of degradation of a specific protein during a period of change in protein amount is described. The calculation uses measurements of rates of synthesis and amount of the protein, together with one estimate of the protein half-life. The method is demonstrated by using data from measurements made on the accumulation of fatty acid synthetase in hormonally treated explants of mammary gland from mid-pregnant rabbits, and data from other authors. These calculations demonstrate a regulated programme of co-ordinated changes in rates of synthesis and degradation during a period of change in protein mass. The role of such changes in protein degradation during protein accumulation is discussed.
D000818:Animals; D064429:Fatty Acid Synthases / Q000378:metabolism; D005260:Female; D006207:Half-Life; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000201:enzymology; D008722:Methods; D008954:Models, Biological; D011247:Pregnancy; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits
D011506:Proteins; D064429:Fatty Acid Synthases