Metabolism of [14C] amitraz in larvae of Boophilus microplus.
Amitraz, 1, 5-di(2, 4-dimethylphenyl)-3-methyl-1, 3, 5-triazapenta-1, 4-diene, labelled with 14C in the 2-methyl groups was applied to B. microplus larvae by an immersion technique. The chemical penetrated readily but never appeared in large amounts internally due to rapid cleavage to N-2, 4-dimethylphenyl-N'-methylformamidine. The expected complementary cleavage product 2, 4-dimethylformanilide was not produced in equivalent quantity. However, large amounts of polar metabolite(s) were produced. Small quantities of 2, 4-dimethylaniline and an unidentified non-polar metabolite were also produced. Of the identified chemicals only amitraz and N-2, 4-dimethylphenyl-N'-methylformamidine were toxic to larvae. Piperonyl butoxide applied simultaneously with amitraz had only a slight effect on metabolism but had a three-fold synergistic effect. SKF 525-A similarly applied had a negligible effect on both metabolism and toxicity.
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D004351:Drug Resistance; D004357:Drug Synergism; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D010882:Piperonyl Butoxide; D011335:Proadifen; D013987:Ticks / Q000378:metabolism*; D014052:Toluidines / Q000378:metabolism*
D007306:Insecticides; D014052:Toluidines; D002245:Carbon Dioxide; C014983:amitraz; D011335:Proadifen; D010882:Piperonyl Butoxide