Emergency gas-chromatographic assay of phenobarbital and phenytoin and liquid-chromatographic assay of theophylline.
Phenobarbital and phenytoin are extracted and concentrated in a single step, without solvent evaporation, followed by on-column methylation and gas-chromatographic quanitation. A similar extraction step for the assay of theophylline by high-pressure liquid chromatography is described. The extraction step can be completed in less than 2 min, the chromatographic step in less than 8 min. This extraction method yields clean chromatograms, avoids evaporating health-hazardous solvents, and is applicable to other drugs.
D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004630:Emergencies; D006801:Humans; D010634:Phenobarbital / Q000097:blood*; D010672:Phenytoin / Q000097:blood*; D013806:Theophylline / Q000097:blood*
D010672:Phenytoin; D013806:Theophylline; D010634:Phenobarbital