[Extracellular lipases of the fungus Rhizopus microsporus UzLT-1].
Lipase biosynthesis by the fungus Rhizopus microsporus UzLT-1 was investigated. Under certain conditions the fungus could produce two forms of extracellular lipases. The paper describes the process of purification of the enzyme L-I,N- and C-terminal amino acids of the lipases have been determined. Identity of terminal amino acids of both enzymes has been demonstrated. It has been shown that both forms of lipase from Rhizopus microporus hydrolyze predominantly alpha-ester bonds.
D000596:Amino Acids / Q000032:analysis; D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification / Q000378:metabolism*; D012233:Rhizopus / Q000201:enzymology*; D013379:Substrate Specificity
D000596:Amino Acids; D008049:Lipase
Izuchenie vnekletochnykh lipaz griba Rhizopus microsporus, UzLT-1.