Incorporation rates of [1-14C]glycerol into the molecular species of alkyl ether phospholipids of Ehrlich ascites tumor cells in vivo.
[1-14C]Glycerol was injected into the peritoneal cavity of mice bearing Ehrlich ascites tumor cells and the lipids were extracted from the cells after selected periods. 1-O-Alkyl-2-acyl-3-acetyl-glycerols and 1,2-diacyl-3-acetylglycerols were prepared from the ethanolamine and choline phosphoglycerides and fractionated by AgNO3-impregnated thin-layer chromatography into seven molecular species. Bands 1--7 obtained in this way can be designed as saturated, monoene, diene (11), diene (02), triene, tetraene and pentaene + hexaene species, respectively. Band 7, composed mainly of hexaene species, was predominant among those derived from ether phospholipids. The predominant species was band 4 (saturated/linoleate) in both diacyl ethanolamine and choline phospholipids. The specific radioactivities of the fractionated species were determined. In diacyl or alkyl ether compounds of ethanolamine and choline phosphoglycerides, disaturated species turned over very rapidly, while in alkyl ether ethanolamine phosphglyceride, hexaene + pentaene molecular species turned over rapidly. In diacyl compounds of the ethanolamine phosphoglyceride, hexaene molecular species also turned over at a high rate. In diacyl ethanolamine or choline phosphoglycerides, dienoic species turned over rather slowly.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D005990:Glycerol / Q000378:metabolism*; D008297:Male; D051379:Mice; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010714:Phosphatidylethanolamines / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis*; D013329:Structure-Activity Relationship
D005227:Fatty Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D005990:Glycerol