Characterization of uterine heterogeneous nuclear ribonucleic acid and the effect of oestradiol-17 beta on its synthesis.
An early response to the administration of oestradiol-17 beta to immature rats is a dramatic stimulation in the synthesis of uterine hnRNA (heterogenous nuclear RNA). High-molecular-weight fractions of the hnRNA were purified and subfractionated on poly(U)-Sepharose into fractions that differed in their poly(A) content and their size profile on polyacrylamide gels. Oestrogen treatment of the rats stimulated the synthesis of all three fractions of high-molecular-weight hnRNA, but the kinetics of synthesis, degree of stimulation and size distribution of the newly synthesize RNA differed in each fraction.
D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D008970:Molecular Weight; D012313:RNA / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D004958:Estradiol; D012313:RNA