Studies of microhigh-performance liquid chromatography. IV. Application of the micro pre-column method to the analysis of corticosteroids in serum.
The micro pre-column method, which is a useful method for the pre-treatment of samples, was applied to the analysis of corticosteroids in serum. Corticosteroids were concentrated in a micro pre-column packed with porous styrene-divinylbenzene copolymer particles and separated in a micro separation column packed with porous silica particles. Concentrations of corticosterone, cortisone and cortisol in the 20-130 ppb range were determined individually by pre-treatment of only 0.2 ml of serum.
D000305:Adrenal Cortex Hormones / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D006736:Horses; D008832:Microchemistry; D011817:Rabbits
D000305:Adrenal Cortex Hormones