title: Distribution of surface coat material on fusing neural folds of mouse embryos during neurulation.
abstract: Fusing and non-fusing regions of neural folds from mouse embryos were examined during neurulation for the distribution of extracellular macromolecules (surface coats) prior to and at the time of closure. Ruthenium red staining of 10th day ICR/DUB mouse embryos was used to detect the distribution of surface coat material. Light microscopic examination of fusing and non-fusing regions in the midbrain, hindbrain, and spinal cord showed a consistent increase in ruthenium red positive material immediately prior to closure. Heavy deposits of positive staining material were present along apical neural fold borders and overlying ectoderm cells. This staining pattern was consistent in the three regions examined, but the pattern of initial contact between opposing neural folds differed. In mid- and hindbrain areas contact was initiated by overlying ectoderm, whereas in spinal cord regions contact was first established by neuroepithelial cells. Once contact between opposing neural folds was initiated a decrease in stainable material was observed.
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000196:embryology*; D004847:Epithelial Cells; D005110:Extracellular Space; D005260:Female; D046911:Macromolecular Substances; D008297:Male; D051379:Mice / Q000196:embryology*; D011247:Pregnancy; D013499:Surface Properties
chemical_list: D046911:Macromolecular Substances