Dansylaziridine-labeled troponin C. A new fluorescent "physiological" probe for calcium regulation by sarcoplasmic reticulum.
Dansylaziridine-labeled troponin C (TnCDANZ) undergoes a greater than 2-fold fluorescence enhancement with Ca2+ binding to the Ca2+-specific regulatory sites of troponin C. Hence, TnCDANZ serves as a very good indicator of Ca2+ in the range of 3.0 to 70 micron. Ca2+ uptake and release by sarcoplasmic reticulum can be easily and accurately monitored by the fluorescence changes in TnCDANZ. In this manner, we can monitor directly the removal of Ca2+ from troponin C by the sarcoplasmic reticulum. Thus, this reaction represents a useful analogue ot the Ca2+ "on-off" process in muscle.
D000818:Animals; D001388:Aziridines; D001389:Azirines; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D003619:Dansyl Compounds; D004285:Dogs; D007700:Kinetics; D009114:Murexide / Q000494:pharmacology; D009124:Muscle Proteins / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014336:Troponin / Q000378:metabolism
D001388:Aziridines; D001389:Azirines; D003619:Dansyl Compounds; D009124:Muscle Proteins; D014336:Troponin; D009114:Murexide; D002118:Calcium