High-performance liquid chromatographic assay of sulfapyridine and acetylsulfapyridine in biological fluids.
A high-pressure liquid chromatographic method for the sensitive, rapid, and specific determination of sulfapyridine and its N-acetyl derivative in plasma and saliva was developed. A cyano-bonded, reversed-phase, high efficiency column was used. The system detected these sulfonamides in serum to 0.25 mg/liter and within only 6 min. Sulfapyridine was separated from its acetyl derivative with little interference from other drugs. The assay reproducibility was within 3%. The assay was highly useful for routine monitoring of patients receiving sulfasalazine for inflammatory bowel disease.
D000107:Acetylation; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D005069:Evaluation Studies as Topic; D006801:Humans; D008722:Methods; D012463:Saliva / Q000032:analysis; D013053:Spectrophotometry; D013424:Sulfanilamides / Q000032:analysis*; D013427:Sulfapyridine / Q000032:analysis* / Q000097:blood
D013424:Sulfanilamides; D013427:Sulfapyridine