[Specific blast transformation of rabbit spleen cells induced by a bacterial polysaccharide and inhibitory effect of peripheral blood lymphocytes (author's transl)].
Following immunization with bacteria (i.e. Salmonella johannesburg), rabbit spleen lymphocytes developed a specific blast response when the lymphocytes were stimulated with polysaccharide, the haptenic moiety of lipopolysaccharide. A clear cut dissociation was noted in the blast response induced by polysaccharide compared with those induced by lipopolysaccharide and lipid A. There was no correlation between the magnitude of the cellular responses and that of the antibody response. Moreover, there was less specificity at the cellular level than at the level of antibody secreted by cells. A decrease of 3H-thymidine incorporation was often observed after immunization, at the level of peripheral blood lymphocytes. An inhibitory effect of these cells was shown on the blast response of spleen lymphocytes with polysaccharide. A high blast response to Salmonella polysaccharide which could be observed in some non-immunized rabbits might be related to a natural sensitization of animals with the same or related unknown antigens which could not be recognized by anti-S. johannesburg antibodies.
D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D007114:Immunization; D008050:Lipid A / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D011817:Rabbits; D012475:Salmonella / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013154:Spleen / Q000276:immunology*; D013997:Time Factors
D008050:Lipid A; D011135:Polysaccharides, Bacterial; D012710:Serum Albumin, Bovine
Transformation blastique spécifique des lymphocytes spléniques de lapins induite par un polyoside bactérien et effet inhibiteur des lymphocytes du sang périphérique.