Cell cycle analysis and synchronization of the TN-368 insect cell line.
A cell cycle analysis of the Trichoplusia ni (TN-368) insect cell line is described. By means of autoradiography and percent labeled metaphase data, the cell cycle parameters were determined to be as follows: S, 4.5 hr; G2, 8.5 hr; M, 0.5 hr; G1, 1.0 hr; the total cell time being 14.5 hr. A synchronization procedure using 50 mM thymidine in a double block procedure was used to provide a method of obtaining a large number of cells in particular cell cycle phases, especially S and G2.
D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D007313:Insecta; D008677:Metaphase; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D004247:DNA; D013936:Thymidine