[Effect of 0.2--0.3M sodium chloride solutions on the properties of the chromatin of lymphocytes in different stages os maturation].
The response of lymph node lymphocytes stimulated to proliferation at different stages of maturation by cell seeding at the high cell density was studied. The binding of acridine orange (A3) and 3H-actinomycin D (3H-AMD) to the cell nuclei was used as a criterion of the functional state of lymphocyte chromatin. The "crowding" stimulation of the lymph node lumphocytes resulted in the increase of AO and 3H-AMD binding by the cells of different sizes, i.e. at different stages of maturation. The degree of binding differs in cells of different size and the increase is expressed to a greater extent in small lymphocytes. The cell treatment with 0.2--0.3M sodium chloride solutions eliminates the differences in AO and 3H-AMD binding between the activated an non-activated lymphocytes of all sizes what appears to be due to the removal of some weakly bound non-histone proteins from the activated cells. The differences in AO and 3H-AMD binding between the cells of different sizes are retained after this treatment. Hence, the proteins removed from the chromatin under the effect of such a treatment are not responsible for the chromatin changes accompanying the cell maturation.
D000166:Acridines / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000187:drug effects / Q000502:physiology*; D004247:DNA / Q000032:analysis; D003609:Dactinomycin / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000032:analysis / Q000187:drug effects / Q000648:ultrastructure*; D009698:Nucleoproteins / Q000502:physiology*; D009994:Osmolar Concentration; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology
D000166:Acridines; D002843:Chromatin; D009698:Nucleoproteins; D003609:Dactinomycin; D012965:Sodium Chloride; D004247:DNA
Vliianie 0.2--0.3 M rastvorov khloristogo natriia na svoĭstva khromatina limfotsitov, nakhodiashchikhsia na raznykh stadiiakh sozrevaniia.