Tumour-necrosis factor from the rabbit. II. Production by monocytes.
Mononuclear cells from normal rabbit blood were cytotoxic to a number of cell lines in vitro. The cytotoxic cells were contained in the monocyte-enriched fraction adherent to plastic. Supernatants of the monocyte-enriched fraction had the same cytotoxic specificity as the parent cells. The cytotoxic factor precipitated in 50% saturated ammonium sulphate solution and on gel filtration was heterogeneous with a mol.-wt. range of 30--50,000 u. On the basis of specificity and molecular characteristics, this cytotoxic factor closely resembles rabbit tumour-necrosis factor (TNF) suggesting that TNF or a closely related factor is a normal product of mononuclear phagocytes.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins; D007739:L Cells / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology*; D011817:Rabbits
D006023:Glycoproteins