[Production of influenza virus recombinations].
Described in this paper is the preparation of influenza virus recombination partners, H3N2 and H3N1, by poly-infection of primary embryonic fowl cells with influenza virus A/Greifswald/6/74 (H3N2) or with A/Greifswald/1/76 (H3N2) and A/PR/8/34 (H0N1). Purification was based on four consecutive plaque isolations. The productivity of all recombination partners isolated in the embryonated fowl egg was higher than that of parent strain, H3N2. A recombination partner of H0N2 was obtained from poly-infection of isolated chorio-allantois membranes (CAM) with inactivated influenza virus A/PR/8/34 (H0N1) and A/Greifswald/6/74 (H3N2), the whole process being exposed to the action of serum anti-H3N1. Purification was undertaken via plaque passages, as well. The recombination partners could be characterised by determination of haemagglutination titres in the embryonated fowl egg and by reference to plaque diameters.
D000482:Allantois; D000818:Animals; D002642:Chick Embryo; D002823:Chorion; D005859:Germany, East; D009154:Mutation; D009975:Orthomyxoviridae / Q000235:genetics* / Q000302:isolation & purification; D011995:Recombination, Genetic; D014776:Virus Cultivation / Q000379:methods
Gewinnung von Influenzavirusrekombinanten.