title: Utilization and cooxidation of chlorinated phenols by Pseudomonas sp. B 13.
abstract: Pseudomonas sp. B13 was grown in continuous culture on 4-chlorophenol as the only carbon source. Maximum growth rate of 0.4 h(-1) was observed at a substrate concentration of greater than 0.01 mM and less than 0.15 mM. In addition to the enzymes of phenol catabolism, high specific 1,2-dioxygenase activities with chlorocatechols as substrates were found. The isomeric monochlorinated phenols were also totally degraded by 4-chlorophenol grown cells. (+)-2,5-Dihydro-4-methyl- and (+)-2,5-dihydro-2-methyl-5-oxo-furan-2-acetic acid were formed in high yield as dead-end catabolites from cooxidation of cresoles. Several dichlorophenols except 2,6-dichlorophenol were removed from the culture fluid by chlorophenol grown cells. Ring cleavage of chlorinated catechols were shown to be one of the critical steps in chlorophenol catabolism. A catabolic pathway for isomeric chlorophenols is discussed.
mesh_terms: D002396:Catechols / Q000378:metabolism; D002474:Cell-Free System; D002733:Chlorophenols / Q000378:metabolism*; D003408:Cresols / Q000378:metabolism; D007535:Isomerases / Q000378:metabolism; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D012722:Sewage; D013237:Stereoisomerism; D014871:Water Microbiology
chemical_list: D002396:Catechols; D002733:Chlorophenols; D003408:Cresols; D012722:Sewage; D010105:Oxygenases; D007535:Isomerases