A simple assay technique for pancreatic lipase.
A simple and inexpensive procedure is described to include pancreatic lipase in the protocol for evaluation of pancreatic function by duodenal aspiration. The method involves dilution with an albumin solution, a 2-hr incubation with a commercially available substrate mixture, and manual titration with a burette. Results comparable to assay with a pH-stat were obtained. Assay for pancreatic lipase is recommended as a part of the diagnostic procedure of duodenal aspiration.
D000328:Adult; D004386:Duodenum; D006801:Humans; D008049:Lipase / Q000032:analysis*; D008297:Male; D008722:Methods; D010189:Pancreatic Juice / Q000032:analysis*
D008049:Lipase