Effect of temperature on the order of electrophoretic migration of influenza virus neuraminidase and nucleoprotein genes in acrylamide gels lacking denaturing agents.
When subjected to electrophoresis at 33 degrees C in 3% polyacrylamide gels with no urea added, the nucleoprotein and neuraminidase genes of an H2N2 and H3N2 virus migrate as RNA bands 5 and 6 respectively. If the temperature of electrophoresis is increased to 46 degrees C, however, this order of migration is reversed.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005814:Genes, Viral; D009980:Influenza A virus / Q000032:analysis / Q000235:genetics*; D009439:Neuraminidase / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*; D011995:Recombination, Genetic; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics*
D012367:RNA, Viral; D014764:Viral Proteins; D009439:Neuraminidase