Recovery of lymphocytes from clotted blood for HLA typing.
A method to recover lymphoid cells from clotted blood for the microcytotoxicity test used in HLA typing is described. The procedure consists of disruption of the clotted blood followed by Ficoll-Hypaque gradient purification of the mononuclear cells. The results of HLA typing of lymphocytes from unclotted and clotted blood were identical.
D001777:Blood Coagulation; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens; D006650:Histocompatibility Testing; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology*
D006680:HLA Antigens