The synthesis of 25-hydroxycholecalciferol-23,23,24,24-t4 of high specific activity.
Catalytic tritium reduction of cholest-5-en-23-yne-3beta,25-diol diacetate (VIII) gave cholest-5-ene-3 beta,25-diol diacetate-23,23,24,24-t4 (IX) having a specific activity of 92 Ci/mmol. Bromination, dehydrobromination and hydrolysis of the labelled material gave cholesta-5,7-diene-3beta,25-diol-23,23,24,24-t4 (XI) which was photolyzed to the previtamin and then thermally equilibrated to 25-hydroxycholecalciferol-23,23,24,24-t4 (I).
D002384:Catalysis; D006887:Hydroxycholecalciferols / Q000138:chemical synthesis*; D007553:Isotope Labeling; D008722:Methods; D014316:Tritium
D006887:Hydroxycholecalciferols; D014316:Tritium