Fluorometric assay for urinary indapamide.
A sensitive fluorescence method for the determination of indapamide was developed. Reaction of indapamide with sodium hydroxide at 100 degrees yielded a fluorescent product, and addition of formaldehyde to the fluorescent product increased its fluorescence intensity by a factor of three. The assay is sensitive to levels of indapamide of 0.025 microgram/ml in an aqueous solution, and a linear response between 0.025 and 2.0 microgram/ml was observed. The procedure was adapted to the analysis of intact indapamide in urine. Concentrations of indapamide of 0.05 microgram/ml can be detected in dogs given 20 mg of the drug.
D000818:Animals; D002855:Chromatography, Thin Layer; D004232:Diuretics / Q000652:urine*; D004285:Dogs; D004355:Drug Stability; D005069:Evaluation Studies as Topic; D007190:Indapamide / Q000652:urine*; D008297:Male; D008722:Methods; D013050:Spectrometry, Fluorescence
D004232:Diuretics; D007190:Indapamide