Derivation and maintenance of gnotobiotic dogs.
Revised techniques for derivation and maintenance of gnotobiotic dogs for research purposes were outlined. Gravid uteri were removed from pregnant beagle bitches and passed through a germicidal trap into a flexible plastic isolation unit. Pups were raised on a milk replacement diet and weaned to solid food by 4 weeks of age.
D000821:Animal Feed; D000818:Animals; D004285:Dogs / Q000254:growth & development / Q000601:surgery; D005260:Female; D005856:Germ-Free Life; D006799:Housing, Animal; D008722:Methods; D011247:Pregnancy; D014599:Uterus / Q000601:surgery