title: Spectrodensitometry of nanogram quantities of cholesterol.
abstract: In studies of hydrolysis and synthesis of cholesterol esters by aortic enzymes, conventional methods (aside from isotope methodology) are too insensitive to measure reaction products when few aortas are used. High-performance thin-layer chromatography coupled with spectrodensitometry of plates charred after spraying with a cupric acetate-phosphoric acid reagent permitted quantitation of 10-ng amounts of cholesterol, cholesterol oleate oleate, and oleic acid. Linear calibration curves were obtained after spectrodensitometry of chromatograms containing 10-200 ng of lipid. The results were verified by radioassay.
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000032:analysis* / Q000096:biosynthesis; D002788:Cholesterol Esters / Q000032:analysis* / Q000096:biosynthesis; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D008297:Male; D008832:Microchemistry; D009829:Oleic Acids; D010179:Pancreas / Q000201:enzymology; D011817:Rabbits; D013053:Spectrophotometry / Q000379:methods; D002787:Sterol Esterase / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D002788:Cholesterol Esters; D009829:Oleic Acids; D002784:Cholesterol; D002787:Sterol Esterase