Establishment and characterization of three new continuous cell lines derived from human breast carcinomas.
Three continuous lines of mammary tumor cells (ZR-75-1, ZR-75-27, and ZR-75-30) have been established from malignant effusions of two women with breast cancer. Differentiated properties expressed by each cell line include: (a) epithelial morphology (by light and electron microscopy) resembling that of the parental tumors; (b) presence of receptors for estrogen and other steroid hormones; and (c) growth responsiveness to estrogen and/or progesterone. All three cell lines possess human karyotypes that differ from one another in modal chromosome number as well as in characteristic marker chromosomes. Two of the cultures (ZR-75-27 and ZR-75-30), although derived from the same patient, have stable differences in their karyotypes.
D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002364:Caseins / Q000096:biosynthesis; D002455:Cell Division; D002460:Cell Line; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007700:Kinetics; D007768:Lactalbumin / Q000096:biosynthesis; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental / Q000473:pathology; D009376:Neoplasms, Hormone-Dependent / Q000378:metabolism; D011987:Receptors, Steroid; D013936:Thymidine / Q000378:metabolism
D002364:Caseins; D011987:Receptors, Steroid; D007768:Lactalbumin; D013936:Thymidine