The response to insulin by adipose tissue of insulin-dependent diabetics in vitro.
Subcutaneous adipose tissue was removed by surgical biopsy from 10 normal subjects and 4 insulin-dependent diabetics under exact intracutaneous anaesthesia. The adipose tissue fragments or isolated fat cells were incubated with a tracer amount of labeled glucose. In the presence of insulin (62.5 muU/ml) the production of carbon dioxide from glucose by adipose tissue fragments of nonobese controls as well as diabetics increased up to 286 +/- 62% and 198 +/- 14.7%, respectively. Furthermore, triglyceride synthesis was raised to 257 +/- 30% and 267 +/- 34%, respectively. There were no differences in the sensitivity of adipose tissue to insulin of healthy volunteers and juvenile diabetics.
D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology* / Q000627:therapeutic use; D014280:Triglycerides / Q000096:biosynthesis
D007328:Insulin; D014280:Triglycerides