Histochemical methods for the demonstration of Thomsen-Friedenreich antigen in cell suspensions and tissue sections.
1. Three different methods are described for the visualisation of the Thomsen-Friedenreich (TF) antigen on cell suspensions, formalin-fixed and paraffin embedded or frozen tissue sections: a) Rosette formation with chicken and sheep erythrocytes. b) Fluorescence-microscopy with fluorescein labelled peanut agglutinin. c) Autoradiography with 3H-labelled peanut agglutinin. 2. The TF antigen was shown, as far as presently investigated, to be exposed on various blood cells, glomerula of the kidney and normal mammary gland after neuraminidase treatment. Mammary gland was also shown to possess TF receptors without prior treatment with neuraminidase. 3. The exposure of this cryptantigen can be brought about by bacterial or viral neuraminidase and is followed by an antigen/antibody reaction, which can lead to possible pathological consequences.
D000818:Animals; D001345:Autoradiography; D001773:Blood Cells / Q000276:immunology; D001940:Breast / Q000166:cytology; D002645:Chickens; D004905:Erythrocyte Aging; D006801:Humans; D007519:Isoantigens / Q000032:analysis*; D037102:Lectins; D008856:Microscopy, Fluorescence; D009439:Neuraminidase; D012397:Rosette Formation; D012756:Sheep
D007519:Isoantigens; D037102:Lectins; D009439:Neuraminidase