Effects of renal fuels on uptake of PAH and uric acid by separated renal tubules of the rabbit.
A rapid-filtration procedure was used to examine the effects of a wide variety of renal fuels on the uptake of p-aminohippuric acid (PAH) and uric acid (UA) by separated rabbit renal tubules. PAH and UA uptakes in 15 min over a range of substrate concentrations of 0.01-10.2 mM were determined. All tricarboxylic acid cycle intermediates and pyruvate showed biphasic stimulation of PAH and UA uptake. alpha-Ketoglutarate produced a 320 +/- 54% increase in PAH uptake and a 192 +/- 60% increase in UA uptake at 0.16 mM, the concentration at which uptake was maximal, while causing 20 +/- 3 (PAH) and 35 +/- 7% (UA) inhibition at 10.2 mM. Citrate produced a 373 +/- 19% increase in PAH uptake and a 246 +/- 41% increase in UA uptake at 0.64 mM. PAH and UA uptake were also stimulated by acetate, glucose frutose, phosphoenolypyruvate and L-glutamic acid. The data indicate a direct relationship between stimulation of PAH and uric acid transport and stimulation of renal cortical oxidative metabolism.
D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002951:Citrates / Q000494:pharmacology; D004734:Energy Metabolism; D005632:Fructose / Q000494:pharmacology; D005650:Fumarates / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D007656:Ketoglutaric Acids / Q000494:pharmacology; D007684:Kidney Tubules / Q000378:metabolism*; D008293:Malates / Q000494:pharmacology; D008297:Male; D010071:Oxaloacetates / Q000494:pharmacology; D011817:Rabbits; D013386:Succinates / Q000494:pharmacology; D014527:Uric Acid / Q000378:metabolism*; D010130:p-Aminohippuric Acid / Q000378:metabolism*
D000618:Aminohippuric Acids; D002951:Citrates; D005650:Fumarates; D007656:Ketoglutaric Acids; D008293:Malates; D010071:Oxaloacetates; D013386:Succinates; D014527:Uric Acid; D005632:Fructose; D005947:Glucose; D010130:p-Aminohippuric Acid