title: Getting started with a scanning transmission electron microscope coupled to a small computer.
abstract: A scanning transmission electron microscope (STEM) equipped with a laser-heated gun was coupled to a small computer. Several alterations to the commercially obtained parts of this system are described. On-line operation procedures were developed aiming to reduce the amount of radiation of the specimen area of interest. The resulting system is capable of recording information at the 1.0 to 1.5 nm resolution level by taking advantage of the efficiency of a STEM in recording the information and in controlling the irradiation conditions. These features are important in the study of biological material.
mesh_terms: D003201:Computers; D007834:Lasers; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000592:standards*