[Study of "bound insulin" of the blood sera of blood/donors and patients with diabetes mellitus by circular dichroism].
Circular dichroism was applied to the study of the structure of the insulin-transferrin complexes ("bound insulin") isolated from the blood sera of donors and patients suffering from diabetes mellitus of moderate severity. There proved to be a considerable (in comparison with the normal) reduction of the alpha-helix areas in the "bound insulin"molecule of the patients. A comparative study of the circular dichroism spectra in the area of absorption of aromatic amino acids permitted to suppose that the structural changes of the molecule of a complex isolated from the blood sera of patients could not be explained by alterations in the area of the aromatic amino acids.
D001782:Blood Donors; D002942:Circular Dichroism; D003920:Diabetes Mellitus / Q000097:blood* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007328:Insulin / Q000097:blood* / Q000378:metabolism; D011485:Protein Binding; D014168:Transferrin / Q000378:metabolism
D007328:Insulin; D014168:Transferrin
Izuchenie "sviazannogo insulina" syvorotok krovi donorov i bol'nykh sakharnym diabetom metodom krugovogo dikhroizma.