[Microestimation of haemoglobins A2 and F by measurement of their peroxydase activity (author's transl)].
A microtechnique of evaluation for the haemoglobins A2 and F is presented, in which the final measurement is made more sensitive by evaluation of peroxidatic activity. Haemoglobin A2 is previously separated by chromatography on a very small column of DEAE cellulose and haemoglobin F is obtained through a microtechnique of alkaline precipitation. The sensitivity of this evaluation is increased by a factor of 20.25 microliter of hemolyzate are enough for a determination. Results obtained are in accordance with those given by the classical methods.
D005319:Fetal Hemoglobin / Q000032:analysis*; D006441:Hemoglobin A / Q000032:analysis*; D006452:Hemoglobinometry / Q000379:methods; D006801:Humans; D008832:Microchemistry / Q000379:methods; D010544:Peroxidases / Q000097:blood*; D012016:Reference Values
D006441:Hemoglobin A; D005319:Fetal Hemoglobin; D010544:Peroxidases
Microdosage des hémoglobines A2 et F par la mesure de leur activité peroxydasique.