Serum levels of 8-methoxypsoralen in psoriasis patients using a new fluorodensitometric method.
A method is described for the determination of 8-methoxypsoralen in human serum using a fluorodensitometric method. The fluorescence densitometry was carried out directly on the thin layer plate. The developing fluid consisted of chloroform/methanol. In patients with psoriasis after oral application of 8-methoxypsoralen the peak serum levels are mostly found between 2 and 3 h. One milliliter serum is sufficient to determine 8-methoxypsoralen levels. The results are in accordance with those obtained with gas chromatographic methods.
D002855:Chromatography, Thin Layer; D003720:Densitometry; D005453:Fluorescence; D006801:Humans; D008730:Methoxsalen / Q000097:blood* / Q000627:therapeutic use; D011565:Psoriasis / Q000097:blood* / Q000188:drug therapy
D008730:Methoxsalen