Analysis for salicylic acid in serum by high-performance liquid chromatography.
We report a specific and sensitive quantitative assay for salicylate in serum or plasma. As little as 3 microliter of sample can be analyzed by an external standard procedure and larger volumes by diluting with the internal standard (o-methoxybenzoic acid). No extraction or derivatization is necessary and salicylic acid and the internal standard elute in 6 and 3.75 min, respectively. Peak-height ratios are linearly related to concentrations between 10 and 500 mg/liter. By comparison with an existing fluorometric method, our procedure is faster, more specific, and more sensitive.
D001241:Aspirin / Q000097:blood*; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007223:Infant; D008832:Microchemistry; D012459:Salicylates / Q000097:blood*
D012459:Salicylates; D001241:Aspirin