Delayed hypersensitivity to soluble antigens in mice. I. Analysis in vivo.
Delayed hypersensitivity to a soluble protein has been analyzed in mice. Ear swelling, 24 h after injection of a test dose of antigen, has been used to monitor the response to a subcutaneous tail injection of egg albumin in complete Freund's adjuvant. Maximal 24 h ear swelling was observed 8 days after sensitization with either 50 or 100 microgram of antigen. These delayed reactions, which were characterized by mononuclear infiltrates, were relatively uncomplicated by Arthus reactions. The latter, antibody-mediated reactions were apparent at later times after immunization. More intense 24 hr reactions were elicited in C57BL/6 than BALB/c mice. Passive transfer of sensitivity has been accomplished with lymph node cells.
D000818:Animals; D000941:Antigens; D003520:Cyclophosphamide / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D004423:Ear; D004487:Edema / Q000209:etiology; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007116:Immunization, Passive; D007700:Kinetics; D051379:Mice; D012995:Solubility; D013997:Time Factors
D000941:Antigens; D003520:Cyclophosphamide