Improved thin-layer chromatographic method in the diagnosis of mannosidosis.
An improved thin-layer chromatographic method is described for the facile separation of neutral oligosaccharides excreted in the urine of patients with mannosidosis. The urine sample is treated with mixed-bed ion-exchange resin to remove charged species. The eluate is then chromatographed on silica gel thin-layer plates with n-propanol water as the developer. Eleven unique orcinol-positive components can thus be resolved. The advantages of this method over previously described techniques are the ease and rapidity of assay, better resolution of components, and clarity of resolution. It should be applicable to other disease states in which distinctive neutral carbohydrate products are produced.
D002239:Carbohydrate Metabolism, Inborn Errors / Q000175:diagnosis* / Q000652:urine; D002855:Chromatography, Thin Layer / Q000379:methods; D006801:Humans; D008358:Mannose / Q000378:metabolism*; D008361:Mannosidases / Q000172:deficiency*; D009844:Oligosaccharides / Q000652:urine*
D009844:Oligosaccharides; D008361:Mannosidases; D008358:Mannose