title: Location of ribosome-binding sites on the tetracycline resistance transposon Tn10.
abstract: Eight ribosome-binding sites were located on the single-stranded Tn10 DNA loop which was formed after denaturation of lambda phage DNA containing the Tn10 transposon sequence. Ribosomes were bound only to the Tn10 loop contained on the R strand of lambda DNA but not to that on the L strand, suggesting that one of the two strands of Tn10 DNA is selectively transcribed. Six of the eight ribosome binding sites were located in one-half of the DNA loop. The maximum sizes of potential polypeptides were calculated for these genes to range between 9,500 and 84,000 daltons.
mesh_terms: D001665:Binding Sites; D003090:Coliphages / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004352:Drug Resistance, Microbial; D005796:Genes; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010957:Plasmids; D012270:Ribosomes / Q000378:metabolism*; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D013752:Tetracycline