Effects of delta9-THC on the synaptosomal uptake of 3H-tryptophan and 3H-choline.
The in vitro addition of (-)-delta9-tetrahydrocannabinol (delta9-THC) resulted in a dose-responsive inhibition of the high-affinity specific synaptosomal uptake of both 3H-tryptophan and 3H-choline in mouse forebrain crude synaptosomal preparations. The approximate concentrations of delta9-THC required to cause a 50% inhibition of the uptake of 3H-tryptophan and 3H-choline were 33 and 16 muM, respectively. Kinetic analysis showed that inhibition of both compounds were consistent with a noncompetitive mechanism. The pretreatment of mice with doses of 10, 30 or 100 mg/kg delta9-THC had no effect on the subsequent in vitro synaptosomal uptake of either 3H-tryptophan or 3H-choline into forebrain synaptosomes.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002794:Choline / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D013759:Dronabinol / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium; D014364:Tryptophan / Q000378:metabolism*
D014316:Tritium; D013759:Dronabinol; D014364:Tryptophan; D002794:Choline