Use of laser-nephelometer to quantitate lipoprotein-bound immunoglobulins in human sera. Importance of the diluting reagent.
If the method using Hyland LAS-RTM kit reagent recommended by the manufactorer yielded excellent results to measure immunoglobulins free in serum, the same is not true when one quantitates immunoglobulins bound with antigens, especially with lipidic antigens. Since the specificity of Hyland antisera is not the cause of this difference, we have studied therefore the effect of the diluting buffer on the antisera, on the blank, and its consequences on the assay. The abnormalities encountered in the results were due to precipitation of lipoproteins and immune complexes by the diluting buffer yielded in the kit. We avoided entirely these errors by preparing a blank, not in NaCl 0.15 M, but in the kit reagent used to dilute the antisera.
D000936:Antigen-Antibody Complex; D006801:Humans; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007202:Indicators and Reagents; D007834:Lasers; D008074:Lipoproteins / Q000276:immunology*; D010783:Photometry; D011108:Polymers; D012996:Solutions
D000936:Antigen-Antibody Complex; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007202:Indicators and Reagents; D008074:Lipoproteins; D011108:Polymers; D012996:Solutions