Effect of succinylation on the oligomeric structure of arachin.
Arachin succinylated to various levels has been studied by the techniques of sedimentation velocity, viscosity, absorption and fluorescence spectra, optical rotation and circular dichroism. Succinylation up to 60% leads to the dissociation of the parent 14S molecule to 9S and 4S fractions; above 60% succinylation, 4S fraction occurs predominantly. The other measured properties also show a gradual change up to 50% succinylation and a marked change above this level. The observed changes suggest that a new organised structure is formed before the 4S fraction is denatured.
D010367:Arachis; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D010940:Plant Proteins; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013386:Succinates; D014461:Ultracentrifugation; D014783:Viscosity
D010940:Plant Proteins; D013386:Succinates