Split dose cytotoxic experiments with misonidazole.
The toxicity of misonidazole (1-(2-nitroimidazol-1-yl)-3-methoxy-2-propanol) towards mammalian cells in vitro has been determined as a function of O2 tension. Misonidazole under hypoxic conditions (less than 10 Parts/10(6) O2) shows the greatest toxicity. Split-dose experiments indicate that lethal damage can be "repaired" by O2, the magnitude of this repair being time dependent and a function of O2 concentration, with maximum repair in air seen after 2 h at 37 degree C. Unlike radiation damage this repair is not inhibited by modest hyperthermia (41 degrees C) during the split-dose interval. The implication of these results as regards the mechanism of misonidazole toxicity under anaerobic conditions is discussed.
D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004334:Drug Administration Schedule; D008920:Misonidazole / Q000633:toxicity*; D009593:Nitroimidazoles / Q000633:toxicity*; D010100:Oxygen; D013696:Temperature; D013997:Time Factors
D009593:Nitroimidazoles; D008920:Misonidazole; D010100:Oxygen