Suppression of 3H-acetylcholine release from primary nerve cell cultures by tetanus and botulinum-A toxin.
Primary nerve cell cultures derived from embryonic rat central nervous system form [3H]ACh from exogenous [3H]Ch, and release it upon potassium depolarization. Pretreatment of the cultures with botulinum-A toxin or tetanus toxin diminishes the cellular accumulation of [3H]ACh. Poisoning the cultures during the period of [3H]Ch uptake fails to lower [H]ACh formation. Dependent on dosage, both toxins suppress the release of [3H]ACh upon potassium depolarization. Heat-denaturated toxins as well as tetanus toxin preincubated with tetanus antitoxin were without effect.
D000109:Acetylcholine / Q000096:biosynthesis / Q000378:metabolism*; D000818:Animals; D001905:Botulinum Toxins / Q000494:pharmacology*; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003864:Depression, Chemical; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D013744:Tetanus Toxin / Q000494:pharmacology*
D013744:Tetanus Toxin; D002795:Choline O-Acetyltransferase; D001905:Botulinum Toxins; D000109:Acetylcholine