The anemia of chronic renal failure: studies of the effect of organic solvent extraction of serum.
CRF serum contains a material(s) which is inhibitory to erythropoiesis in vitro. The chemical nature and mechanism of action of this material are unclear at the present time. To further study this problem, we extracted NL serum and CRF serum samples with two organic solvents, chloroform and petroleum ether. The serum samples were studied before and after extraction in a tissue culture system in which dog erythroblasts were stimulated by EP to synthesize heme. Before extraction, cells suspended in CRF serum synthesized less heme than cells cultured in NL serum. Extraction of CRF serum with chloroform, but not petroleum ether, resulted in an improvement in its ability to support heme synthesis. Studies of the material extracted by chloroform from CRF serum showed that it was inhibitory to erythropoiesis. We propose that the erythropoietic inhibitory substance in uremic serum in soluble in chloroform but not in petroleum ether, suggesting that it is a polar lipid.
D000740:Anemia / Q000097:blood* / Q000209:etiology; D001806:Blood Urea Nitrogen; D002725:Chloroform; D004920:Erythropoiesis; D004987:Ethers; D006418:Heme / Q000096:biosynthesis; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000150:complications; D010578:Petroleum; D012997:Solvents
D004987:Ethers; D010578:Petroleum; D012997:Solvents; D006418:Heme; D002725:Chloroform