Emergency determination of acetaminophen.
I describe a senstive colorimetric micromethod in which a pH 11.0 carbonate buffer is used to induce acetaminophen to react with Folin-Ciocalteau reagent at room temperature. These reagents and a simple solvent extraction favor formation of a stable indophenol dye with acetaminophen, giving this procedure a good degree of specificity. Results correlate well with those by liquid-chromatographic procedures, and day-to-day precision is less than 8%.
D000082:Acetaminophen / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003124:Colorimetry / Q000379:methods; D004630:Emergencies; D006801:Humans; D007202:Indicators and Reagents
D007202:Indicators and Reagents; D000082:Acetaminophen