A practical proposal for arginine-vasopressin radioimmunoassay.
This work proposes the use of (a) a commercially available homologous system AVP antibody-AVP (Arginine Vasopressin) standard and (b) new acquisitions for the improvement of sensitivity for AVP radioimmunoassay by separate or simultaneous use of two-phase sequential incubation and epsilon-aminocaproic acid (EACA). The antiserum used in the system described is very specific since none even cross-reacted with lysine vasopressin (LVP) and has an apparent affinity constant (K) of 0.909 +/- 0.047 X 10(12) l/mol. This is sufficiently high to detect 0.5 +/- 0.2 pg/tube, which is theoretically expected of biological AVP. The total assay time is less than 48 h.
D015119:Aminocaproic Acid; D000918:Antibody Specificity; D001127:Arginine Vasopressin / Q000032:analysis* / Q000097:blood / Q000652:urine; D001984:Bronchial Neoplasms / Q000652:urine; D002606:Charcoal; D006801:Humans; D007457:Iodine Radioisotopes; D008722:Methods; D011863:Radioimmunoassay; D013997:Time Factors
D007457:Iodine Radioisotopes; D001127:Arginine Vasopressin; D002606:Charcoal; D015119:Aminocaproic Acid