title: Microdetermination of caffeine in blood by gas chromatography--mass spectrometry.
abstract: A micromethod for the quantitative analysis of caffeine present in small quantities (100 microliter) of whole blood is described. It is based on the gas chromatographic--mass spectrometric analysis of chloroform extracts of biological samples. The method is relatively simple, rapid, specific and sensitive; as little as 20 ng of caffeine can be measured.
mesh_terms: D000818:Animals; D002110:Caffeine / Q000097:blood*; D002849:Chromatography, Gas; D008297:Male; D013058:Mass Spectrometry; D008832:Microchemistry / Q000379:methods; D051381:Rats
chemical_list: D002110:Caffeine