The ultrastructural localization of factor VIII-antigen in human platelets, megakaryocytes and endothelial cells utilizing a ferritin-labelled antibody.
By means of electron microscopy combined with the use of monospecific anti-factor VIII-antigen (VIIIR:AG) antibodies conjugated to ferritin, the subcellular localization of VIIR:AG in platelets, megakaryocytes and in endothelial cells has been established. The reported results suggest the possibility that the megakaryocyte is able to synthesize VIIIR:AG and also to secrete it by means of a microcanalicular system similar to that present in the endothelial cell. Platelets may derive their VIIIR:AG content partly from the megakaryocyte and partly from the plasma.
D000941:Antigens / Q000032:analysis*; D001792:Blood Platelets / Q000276:immunology* / Q000648:ultrastructure; D004727:Endothelium / Q000276:immunology* / Q000648:ultrastructure; D005169:Factor VIII / Q000276:immunology*; D005293:Ferritins; D006801:Humans; D008533:Megakaryocytes / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron
D000941:Antigens; D005169:Factor VIII; D005293:Ferritins