Modified method of erythropoietin (ESF) bioassay in vitro using mouse fetal liver cells. I. Effect of serum iron on 59Fe incorporation into heme.
Investigations on the mouse fetal liver cell bioassay for erythropoietin (ESF) have revealed that iron present in test sera significantly dilutes the radiolabel (59Fe) and thus decreases 59Fe incorporation into heme. A method of correction for the influence of iron on the dose-response relationship of human sera is presented. Application of this method made it possible to assay human sera up to culture concentrations of 150 microliter/ml. It was shown that a corrected serum dose-response curve showed parallelism to the curve of an ESF standard preparation. This suggests similarity of the active principles and allows valid estimation of a potency ratio.
D004305:Dose-Response Relationship, Drug; D004921:Erythropoietin / Q000097:blood*; D006418:Heme / Q000378:metabolism*; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*
D004921:Erythropoietin; D006418:Heme; D007501:Iron