title: Cleavage of a specific bond in troponin C by thrombin.
abstract: Limited proteolysis of rabbit skeletal troponin C with bovine thrombin yielded two fragments, TH1 (Mr = 11000) containing Ca2+ binding regions I--III and TH2 (Mr = 6000) containing region IV. Determination of the partial sequences of the fragments established the site of cleavage at Arg120-Ala121. Secondary cleavage by thrombin at other arginyl or lysyl residues in troponin C was ruled out by the sequence data and by the amino acid compositions of the two fragments.
mesh_terms: D000409:Alanine; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001120:Arginine; D002118:Calcium; D002268:Carboxypeptidases; D002352:Carrier Proteins; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D008239:Lysine; D009124:Muscle Proteins / Q000378:metabolism*; D010446:Peptide Fragments; D011817:Rabbits; D013917:Thrombin / Q000378:metabolism*; D014336:Troponin / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D002352:Carrier Proteins; D009124:Muscle Proteins; D010446:Peptide Fragments; D014336:Troponin; D001120:Arginine; D002268:Carboxypeptidases; D013917:Thrombin; D008239:Lysine; D000409:Alanine; D002118:Calcium