D-Proline: stereospecificity and sodium chloride dependence of lethal convulsant activity in the chick.
Two- and five-day old chicks were injected intraventricularly with D-proline and structurally related compounds. D-proline produced convulsions and lethality, but was non-amnestic, whereas the naturally-occurring isomer, L-proline, was non-convulsant and non-toxic but amnestic. D-proline convulsions were accompanied by decreased high frequency in the EEG and increased slow wave activity. High amplitude spiking was not observed. The lethality of D-proline was saline-dependent. Control experiments ruled out possible toxic factors such as hypertonicity, pH pyrogens, injection volume, or needle misplacement. The results demonstrate that saline and distilled water are not equivalent injection vehicles. A sodium-free vehicle may lead to artifacts but is advantageous in experiments in which amino acid transport must be minimized.
D000818:Animals; D002645:Chickens; D003292:Convulsants; D004347:Drug Interactions; D004569:Electroencephalography; D008297:Male; D011392:Proline / Q000031:analogs & derivatives / Q000494:pharmacology* / Q000633:toxicity; D012965:Sodium Chloride / Q000502:physiology*; D013237:Stereoisomerism
D003292:Convulsants; D012965:Sodium Chloride; D011392:Proline