[Effect of hypothermia on metabolism in the liver during its preservation].
Twenty experiments were conducted on dogs. The effect of hypothermia of different degree (from 18 to 20 degrees C and from 4 to 6 degrees C) on the carbohydrate metabolism and the extent of solubilization of hepatic enzymes (lactate dehydrogenase, glutamate dehydrogenase, urokaninase, DNA-ase, glucose-6-phosphatase) in prefusion-free preservation of the liver was studied. The preservation efficacy was assessed during the subsequent two-hour normothermic perfusion. A marked solubilization of the enzymes under study followed preservation of the liver at 18--20 degrees C; this indicated the loss of intactness of the cell membranes during the preservation. A moderate expenditure of the glycogen stores in the liver, and of sugar in the perfusate followed preservation of the liver at a temperature of 4--6 degrees C; this suggested an even suppression of hepatic metabolism and the prevalence of normal tissue respiration over glycolysis in the restoration of circulation in the liver.
D000818:Animals; D050260:Carbohydrate Metabolism; D002463:Cell Membrane Permeability; D003080:Cold Temperature; D004285:Dogs; D005947:Glucose / Q000378:metabolism; D005958:Glucosephosphates / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008112:Liver Glycogen / Q000378:metabolism; D009926:Organ Preservation; D011773:Pyruvates / Q000378:metabolism
D005958:Glucosephosphates; D007773:Lactates; D008112:Liver Glycogen; D011773:Pyruvates; D005947:Glucose
Vliianie gipotermii na metabolizm v pecheni pri ee konservatsii.