Immunoglobulins on the surfaces of tetrathyridia of Mesocestoides corti Hoeppli 1925 (Cestoda), from laboratory infections of ICR mice.
7Sgamma2b antibody was detected, by fluorescein labeled antibodies, on the body surfaces and wall of excretory bladder of tetrathyridia of M. corti removed from the peritoneums of 5--24-week laboratory infections of ICR/TIMCO mice. 7Sgamma1, 7Sgamma2a, 7Sgamma3, IgM, IgA, and C3 were not found by use of the same techniques. Tetrathyridia maintained in culture medium to which pooled normal mouse, infected mouse, and horse (control) serums containing all known classes and subclasses of immunoglobulins had been added showed only mouse 7Sgamma2b on their surfaces and wall of the excretory bladder, and then only when incubated in pooled serum from infected mice. No other mouse immunoglobulin classes or subclasses were found attached to the worms, nor was any reaction obtained from tetrathyridia incubated in culture medium alone, normal mouse serum, or horse serum. This suggests a highly selective nonspecific absorption of 7Sgamma2b, or more likely demonstrates the specificity of the antibody for the parasite surfaces and wall of the excretory bladder. The possibility that 7Sgamma2b may be acting in an enhanceing mode is discussed.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002589:Cestoda / Q000276:immunology*; D002590:Cestode Infections / Q000276:immunology*; D007136:Immunoglobulins / Q000032:analysis*; D051379:Mice; D008813:Mice, Inbred ICR
D000906:Antibodies; D007136:Immunoglobulins