Enzymic micro-assay for blood glutamine.
Blood glutamine is hydrolyzed by glutaminase; the resulting ammonia is dialyzed out and measured by use of a continuous-flow colorimetric microassay. Concentrations in the range of 10 to 1300 micromol of glutamine per liter can be determined in a 40-microliter sample. With slight modifications, higher concentrations, up to 5 mmol/liter, can be measured. Assay conditions and the qualities of the microtechnique were made optimal. Usual values obtained with this method for arterial and venous blood in man are, respectively, 443 +/- 140 and 466 +/- 142 micromol/liter (mean +/- 2 SD). Corresponding values for rats are 320 +/- 92 and 340 +/- 108 micromol/liter. Fasting causes a decreased blood glutamine.
D000328:Adult; D000368:Aged; D000758:Anesthesia; D000818:Animals; D005019:Ethyl Ethers; D005215:Fasting; D005260:Female; D005972:Glutaminase; D005973:Glutamine / Q000097:blood*; D006801:Humans; D008297:Male; D008722:Methods; D008832:Microchemistry; D008875:Middle Aged; D010424:Pentobarbital; D051381:Rats; D012016:Reference Values
D005019:Ethyl Ethers; D005973:Glutamine; D005972:Glutaminase; D010424:Pentobarbital