title: Methylamine dehydrogenase of Pseudomonas sp. J. Reconstitution from its subunits.
abstract: Conditions for the restoration of catalytic activity from heavy and light subunits which had been isolated from methylamine dehydrogenase of Pseudomonas sp. J were investigated in vitro. Maximal restoration of the activity was obtained in 0.8 M potassium phosphate buffer, pH 7.0-9.3, at 30 degrees C with equimolar concentrations of the two subunits. Under the optimal conditions, the recovery of enzyme activity was 86% and the time required for half-maximal recovery was about 3 min. Addition of bovine serum albumin or p-chloromercuribenzoate to the incubation mixture had no effect on the rate or extent of recovery of the enzyme activity. When the heavy subunit was added to the light subunit, the absorption spectrum of the light subunit changed to a form similar to that observed for the native enzyme. The concentration of methylamine required to change the spectrum of the light subunit was greatly decreased in the presence of the heavy subunit. Reconstituted enzyme was prepared from the isolated subunits and purified by gel chromatography. The reconstituted enzyme resembled the native enzyme in specific activity, molecular weight, substrate specificity, reaction mechanism, Michaelis constants for methylamine and phenazine methosulfate, susceptibility to inhibitor, and absorption, fluorescence and ESR spectra. However, it was less stable than the native enzyme to thermal and pH treatments. The CD spectrum was also slightly different from that of the native enzyme.
mesh_terms: D002942:Circular Dichroism; D004789:Enzyme Activation; D007700:Kinetics; D046911:Macromolecular Substances; D008744:Methylamines; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*; D013053:Spectrophotometry
chemical_list: D046911:Macromolecular Substances; D008744:Methylamines; C021567:methylamine dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors