Automated system for fractionation of blood samples.
We describe a prototype system for preparing multiple fractions of blood components (plasma, washed erythrocytes, and hemolysates) by use of automated techniques. The procedure is based on centrifugal separation and sample transfer by induced pressure differentials in a blood-processing vessel (rotor) that has been designed to process 24 samples simultaneously. Erythrocytes are sedimented against the outer walls of individual sample chambers, and plasma is siphoned, by imposition of either a slight positive or negative pressure, into individual reservoirs in a collection ring. Cells are washed in situ; samples of washed cells, either packed or in saline solution, can be recovered. Cellular hemolysates are automatically prepared and transferred to individual, commercially available collection vials, ready for immediate analysis or for storage in liquid nitrogen. The system has potential application in any biomedical area in which many samples are to be expeditiously analyzed and in which one or more of the blood fractions will be used. A separate unit has been designed and developed for the semiautomated cleaning of the rotor.
D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods*; D005956:Glucose-6-Phosphate Isomerase / Q000097:blood; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D014461:Ultracentrifugation
D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase