Formation of vaccinia virus DNA-protein complex in the presence of isatin beta thiosemicarbazone (IBT).
The association of newly synthesized vaccinia virus DNA with proteins in infected HeLa cells was followed. A shift from a high density to a low density complex occurred between 3 and 6 h after infection. This process was not affected by isatin beta thiosemicarbazone (IBT), an inhibitor of pox virus growth. At 22 h after infection in the absence of IBT, mature virions of high density were observed; however, in the presence of the drug, high density DNA-protein complexes, which lack the two main virus core polypeptides, were formed.
D004279:DNA, Viral / Q000096:biosynthesis; D006367:HeLa Cells; D007211:Indoles / Q000494:pharmacology*; D007510:Isatin / Q000031:analogs & derivatives / Q000494:pharmacology*; D010455:Peptides / Q000032:analysis; D013882:Thiosemicarbazones / Q000494:pharmacology*; D014616:Vaccinia virus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis
D004279:DNA, Viral; D007211:Indoles; D010455:Peptides; D013882:Thiosemicarbazones; D014764:Viral Proteins; D007510:Isatin