[Study of the Sporothrix schenkii (yeast forms) extract. Electrophoretic and immunoelectrophoretic analyses: characterization of enzymatic activities].
An extract from living yeast forms of S. schenckii was prepared. The yeasts originated from a shake culture in B.H.I. broth (Difco) incubated for 3 days at 35 degrees C in darkness; they were harvested, washed and disrupted with glass beads in a model MSK Braun mechanical cell homogenizer; a freezing-thawing was added to improve the extract. After electrophoretic separation in agarose gel, the extract's components were characterized by their enzymic activity; with this technique, 30 bands were revealed. These enzymic activities were also investigated on the antigenic fractions of the extract revealed by a rabbit hyperimmunserum: 16 among 22 immunoprecipitates are identified by their catalytic properties. Study of the earliest precipitating antibodies (appearing-order and enzymic caracterization) in rabbits just immunized completes this work. How to ameliorate the quality of the extract by culture and extraction conditions is also specified.
D002474:Cell-Free System; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003624:Darkness; D004586:Electrophoresis; D004950:Esterases / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D007122:Immunoelectrophoresis; D010088:Oxidoreductases / Q000378:metabolism; D013175:Sporothrix / Q000201:enzymology* / Q000254:growth & development; D013174:Sporotrichosis / Q000382:microbiology*
D010088:Oxidoreductases; D012321:DNA-Directed RNA Polymerases; D004950:Esterases; D006026:Glycoside Hydrolases; D005634:Fructose-Bisphosphate Aldolase
Etude d'un extrait de Sporothrix schenckii (forme levure). Analyse électrophorétique et immunoélectrophorétique; caracterisation des activités enzymatiques.