title: Identification of the 5S RNA binding site in intermolecular complexes of wheat embryo ribosomal 5S and 18S RNA.
abstract: The nucleotide sequence in wheat embryo ribosomal 5S RNA that binds by complementary base pairing to ribosomal 18S RNA has been identified. The 5S--18S RNA complex was formed in vitro and the interacting regions of the two RNA chains were isolated by a combination of nuclease digestion and electrophoresis on polyacrylamide gels. The binding site is close to the 3'-end of the 5S RNA molecule and contains a maximum of 24 nucleotides.
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D046911:Macromolecular Substances; D009693:Nucleic Acid Hybridization; D009843:Oligoribonucleotides; D012335:RNA, Ribosomal / Q000378:metabolism; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis; D014908:Triticum / Q000032:analysis*
chemical_list: D046911:Macromolecular Substances; D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D012265:Ribonucleotides; D012260:Ribonucleases