Comparison of GLC-EMIT analysis for the assay of methadone and its major metabolite in urine.
The enzyme-multiplied immunoassay technique (EMIT) was compared with the gas-liquid chromatographic (GLC) method in the analysis of 130 urine samples from subjects receiving methadone treatment and non-methadone controls. The GC method allowed the quantitation of 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine, and major metabolite of methadone. A good correlation was found between the two methods. The advantages of using the EMIT system are discussed.
D002849:Chromatography, Gas / Q000295:instrumentation; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D008691:Methadone / Q000652:urine*; D008722:Methods
D008691:Methadone