I. An ultrastructural investigation on erythrocytes and lymphocytes coated with proteins: use of CrCl3 as an electron-dense marker.
Proteins (human immunoglobulin G and protein A of Staphylococcus aureus) coupled to the surface of erythrocytes (sheep, mice, man and chicken) and to mouse spleen lymphocytes by chromic chloride technique acquire electron-dense properties and may be visualized in electron microscopy. Sheep red blood cells coated with rabbit anti-erythrocyte antibodies and treated with chromic chloride showed continuous electron-opaque labelling of the cell membrane due to chelation of rabbit IgG with chromium atoms.
D000818:Animals; D002645:Chickens; D002857:Chromium / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000276:immunology / Q000648:ultrastructure; D004912:Erythrocytes / Q000648:ultrastructure*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008214:Lymphocytes / Q000648:ultrastructure*; D051379:Mice; D012756:Sheep; D013205:Staphylococcal Protein A / Q000378:metabolism*
D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D002857:Chromium