Improved separation of nucleosides, nucleotides, and aminoacyl tRNA a strong anion-exchange resin.
Nucleosides and nucleotides can be separated on a polystyrene-dinylbenzene anion-exchange resin, Chromex DA-X8-11 at 50 degree using isocratic elution with 1.0 M ammonium carbonate pH 8.8 or 0.05 M formic acid pH 2.4. The resin can also resolve aminoacyl tRNA on either the analytical or preparative scale.
D000818:Animals; D000837:Anion Exchange Resins; D002852:Chromatography, Ion Exchange; D006868:Hydrolysis; D009705:Nucleosides / Q000302:isolation & purification*; D009711:Nucleotides / Q000302:isolation & purification*; D012343:RNA, Transfer / Q000302:isolation & purification*; D051381:Rats
D000837:Anion Exchange Resins; D009705:Nucleosides; D009711:Nucleotides; D012343:RNA, Transfer