[Use of a histological fixative for extraction of the RNA precursor pool from mollusk nerve tissue].
The incorporation of 3H-uridine into the nucleotide pool of the molluscan nerve tissue was calculated in a routine fixative solution (formalin--alcohol--acetic acid, 9:3:1). The optimal conditions for the precursor output from the tissue being studied, the results obtained well compare with those obtained by common biochemical techniques. Our method gives a possibility to use the same sample of tissue for historadiographical investigation of labeled RNA and for cytochemical determination of nuclear acid amounts.
D000818:Animals; D001345:Autoradiography / Q000379:methods; D005404:Fixatives; D006651:Histocytochemistry / Q000379:methods; D009417:Nerve Tissue / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D012908:Snails / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism*
D005404:Fixatives; D012313:RNA; D014529:Uridine
Ispol'zovanie gistologicheskogo fiksatora dlia ékstraktsii pula predshestvennika RNK iz nervnoĭ tkani molliuskov.