Determination of trimethoprim and sulfamethoxazole (co-trimoxazole) in body fluids of man by means of high-performance liquid chromatography.
A high-performance liquid chromatographic method for the determination of trimethoprim, sulfamethoxazole and its metabolite and a series of structurally related sulfonamides is described. The half-life time of elimination of sulfamethoxazole and its metabolite N4-acetylsulfamethoxazole is 9 h for both compounds. The renal excretion rate of sulfamethoxazole depends strongly on the urinary pH. The renal excretion rate of the metabolite N4-acetylsulfamethoxazole is not dependent on the urinary pH.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D006801:Humans; D013420:Sulfamethoxazole / Q000032:analysis* / Q000097:blood / Q000652:urine; D014295:Trimethoprim / Q000032:analysis* / Q000097:blood / Q000652:urine
D004338:Drug Combinations; D014295:Trimethoprim; D013420:Sulfamethoxazole