A new method of preparation and some properties of 3-hydroxyproline.
The hydrolyzate of Delonix Regia seed extract is fractionated sequentially on Dowex 50 X 8 resin and on QAE Sephadex A 25. Purification is completed by recrystallisation from ethyl alcohol. 3-hydroxyproline is destroyed by NO2H and by chloramine T, which prevents from using most of the colorimetric reactions in use for 4-hydroxyproline. For its characterization, 3-hydroxyproline may be clearly separated from 4-hydroxyproline by TLC chromatography and by high voltage paper electrophoresis.
D002845:Chromatography / Q000379:methods; D004590:Electrophoresis, Paper / Q000379:methods; D006909:Hydroxyproline / Q000032:analysis / Q000302:isolation & purification*; D010944:Plants / Q000032:analysis*; D012639:Seeds / Q000032:analysis
D006909:Hydroxyproline