Isolated, blood-perfused canine arteries: different vasoconstrictor responses of internal and external carotid arteries to 5-hydroxytryptamine.
Canine carotid arteries were isolated, suspended in a bath and perfused under a constant flow rate with arterial blood led from a support dog. Resting perfusion pressure was set at a constant level over 50 mm Hg. Drugs were administered into the endothelial side of the artery through a cannulated tubing and the response was obtained as pressure changes. This preparation was proved to be superior to usual isolated arterial strips, since its reactivity was kept in a stable condition over 5-10 hours at 37 degrees C. Using this preparation, effects of 5-hydroxytryptamine (5-HT) on the internal and external carotid arteries were investigated in comparison with effects of norepinephrine. In the internal carotid artery, 5-HT caused a much more potent vasoconstriction than norepinephrine. On the other hand, in the external carotid artery, 5-HT caused only slight vasoconstriction, while norepinephrine produced a marked vasoconstriction.
D000818:Animals; D001158:Arteries / Q000187:drug effects*; D002342:Carotid Artery, External / Q000187:drug effects; D002343:Carotid Artery, Internal / Q000187:drug effects; D004285:Dogs; D066298:In Vitro Techniques; D009638:Norepinephrine / Q000494:pharmacology; D010477:Perfusion; D012701:Serotonin / Q000494:pharmacology*; D014662:Vasoconstrictor Agents
D014662:Vasoconstrictor Agents; D012701:Serotonin; D009638:Norepinephrine