Composition of proteins of mesenteric lymph chylomicrons in the rat and alterations produced upon exposure of chylomicrons to blood serum and serum proteins.
Protein composition was determined in mesenteric lymph chylomicrons from fat-fed rats. Among the proteins of intermediate molecular weight, apoproteins A-I and the arginine-rich apoprotein accounted for 31% and 4% of the total protein mass, respectively. Apoprotein B and apoprotein A-IV each accounted for about 10% and proteins of low molecular weight (C apoproteins and apoprotein A-II) accounted for most of the remainder. Apoprotein A-I also accounted for more than 30% of the protein mass of mesenteric lymph lipoproteins of density less than 1.006 g/ml ("small chylomicrons") obtained from rats fed glucose. Aproprotein A-I was partially dissociated from chylomicrons during brief ultracentrifugation. Both the arginine-rich apoprotein and the C apoproteins in rat blood serum were transferred to lymph chylomicrons from fat-fed rats during incubation in vitro. Content of arginine-rich apoprotein, determined immunochemically, increased six-fold when chylomicrons were diluted to a final concentration of 500 mg/dl in blood serum. Upon incubation of chylomicrons in equivalent volumes of ultracentrifugal fractions of serum, the increase of the arginine-rich apoprotein was: very low density lipoproteins, 1.5-fold; high density lipoproteins, 1.8-fold; density fraction greater than 1.006 g/ml, 5.0-fold; density fraction greater than 1.21 g/ml, 11-fold. Content of apoprotein A-I, also determined immunochemically, was not altered appreciably by exposure to serum or its ultracentrifugal fractions, whereas content of C apoproteins, estimated from intensity of staining of the low molecular weight protein component in polyacrylamide gel electropherograms, increased in all cases except for the density fraction greater than 1.21 g/ml. The fractional content of apoprotein A-I in the protein of chylomicrons fell after incubation, whereas that of the arginine-rich apoprotein remained constant or rose substantially. The fractional content of apoprotein A-IV in chylomicron-protein tended to follow that of apoprotein A-I, as judged from polyacrylamide gel electropherograms. Transfer of the arginine-rich and C apoproteins to chylomicrons from blood serum was directly related to the volume of serum in which the chylomicrons were diluted and occurred rapidly at room temperature or at 4 degrees C.
D000818:Animals; D001053:Apolipoproteins / Q000378:metabolism; D002914:Chylomicrons / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage; D008074:Lipoproteins / Q000097:blood*; D008196:Lymph / Q000378:metabolism*; D008297:Male; D008643:Mesentery; D008970:Molecular Weight; D011506:Proteins / Q000378:metabolism*; D051381:Rats
D001053:Apolipoproteins; D002914:Chylomicrons; D004041:Dietary Fats; D008074:Lipoproteins; D011506:Proteins