A simple modification to an LKB 8600 reaction rate analyser for rapid measurement of enzymes showing lag phases in a coupled system.
An enzyme assay which utilizes a coupled enzyme system may show a lag phase. To help to overcome problems associated with the kinetic measurement of these enzymes a modification to an LKB 8600 reaction rate analyser is described. It has been applied successfully to a kinetic method for the measurement of 5'-nucleotidase activity in serum.
D055598:Chemical Phenomena; D002621:Chemistry; D004798:Enzymes / Q000097:blood*; D006801:Humans; D007700:Kinetics; D009708:Nucleotidases / Q000097:blood; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation; D013997:Time Factors
D004798:Enzymes; D009708:Nucleotidases