Improved determination of beta-glucuronidase in serum.
With phenolphthalein beta-glucuronide as the substrate in the serum beta-glucuronidase assay, centrifugation at high speed after addition of alkali was required to minimize blanks. Optimal substrate concentration was 3--6 mM. Excess substrate was inhibitory.
D000328:Adult; D000818:Animals; D005966:Glucuronidase / Q000097:blood*; D006801:Humans; D008027:Light; D008297:Male; D008722:Methods; D051379:Mice; D008875:Middle Aged; D010635:Phenolphthaleins; D013053:Spectrophotometry
D010635:Phenolphthaleins; D005966:Glucuronidase