Effects of homologous O-antibody on host responses to lipopolysaccharide from Yersinia enterocolitica: neutralization of its pyrogenicity.
The pyrogenic activity of lipopolysaccharide (LPS) obtained from Yersinia enterocolitica was neutralized by the homologous O-antiserum at the optimal antigen/antibody ratio. The level of neutralization in either antigen excess or extreme antibody excess was significantly lower than that at the optimal ratio. These facts suggested that the structure of the so-called "lattice" of LPS/antibody complex might influence the neutralization of pyrogenic activity. Moreover, when LPS was gradually added to the antiserum to reach the optimal antigen/antibody ratio, the pyrogenic activity of LPS was only slightly neutralized by the antibody. The similar event to Danysz phenomenon that has been known in the diphtheria toxin-antitoxin system was also observed in the LPS-anti-LPS system.
D000818:Animals; D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D004731:Endotoxins / Q000276:immunology; D005260:Female; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009500:Neutralization Tests; D011749:Pyrogens / Q000494:pharmacology*; D011817:Rabbits; D015007:Yersinia / Q000276:immunology*
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D004731:Endotoxins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D011749:Pyrogens