[Growth of Micrococcus lysodeikticus bacteria on a deuterated medium].
The object of this work was to prepare deuterated growth media and to adapt Micrococcus lysodeikticus to a medium containing deuterated-substituted organic substances and deuterium oxide instead of water. M. lysodeikticus was grown on a medium prepared from the "deuterated-cells" of Chlorella, and was capable of absorbing selectively protons from such a medium containing high concentrations of deuterium. Its deuterated cells ("monsters") produced structures consisting of several (up to 8) smaller cells, angular in shape and having a thicker (2--3 times) cell wall. Apparently, adaptation to a deuterated medium is accompanied with changes in the cell wall biosynthesis as a result of which the separation of daughter cells is interfered with in the course of cell division, and the cells are more resistant to the action of lysozyme.
D000222:Adaptation, Physiological; D002708:Chlorella; D003470:Culture Media; D003903:Deuterium / Q000378:metabolism*; D008837:Micrococcus / Q000254:growth & development* / Q000648:ultrastructure; D010940:Plant Proteins / Q000378:metabolism
D003470:Culture Media; D010940:Plant Proteins; D003903:Deuterium
Vyrashchivanie bakteriĭ Micrococcus lysodeikticus na deĭterirovannoĭ srede.