GLC--mass spectrometry of several important anticancer drugs II: doxorubicin and daunorubicin aglycone analogs.
By using previously developed pertrimethylsilylation and methoxime formation procedures, the GLC--mass spectra of pertrimethylsilyl and pertrimethylsilyl methoxime derivatives of 12 doxorubicin and daunorubicin glycone analogs, mostly synthesized in this laboratory, were studied. The fragmentation pathways were readily interpretable, and the patterns were highly specific for corresponding structural changes, thus supporting the previous contention that this method has potential for the identification of doxorubicin and daunorubicin metabolites. Four aglycone metabolites were identified, on the basis of their GLC--mass spectra, in the hydrolysate of the 1-butanol extract of bile obtained from a doxorubicin-treated rabbit.
D000818:Animals; D001646:Bile / Q000378:metabolism; D002849:Chromatography, Gas; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D013058:Mass Spectrometry; D008722:Methods; D011817:Rabbits
D004317:Doxorubicin; D003630:Daunorubicin