A safe blood transfusion procedure for immunization against major histocompatibility complex determinants in man.
A standardized procedure is proposed for deliberate immunizations against human major histocompatibility complex determinants. The data presented demonstrate its effectiveness and, by using a number of necessary precautions, this procedure has proven to be very safe. The following points are especially important: (1) exclusive utilization of regular blood donors as immunizers; (3) use of whole blood as an immunizing agent; and (3) use of small immunizing stimuli rather than large transfusions. This procedure can be recommended for the production of monospecific anti-HLA antisera and it may be useful if and when a deliberate transfusion policy for prospective kidney recipients is adopted.
D001803:Blood Transfusion / Q000379:methods; D005260:Female; D006680:HLA Antigens; D006801:Humans; D007114:Immunization / Q000379:methods*; D008285:Major Histocompatibility Complex
D006680:HLA Antigens