Effects on in vitro tumor growth of murine macrophages isolated from sarcoma lines differing in immunogenicity and metastasizing capacity.
Macrophages were isolated from the strongly immunogenic, non-metastasizing FS6 sarcoma transplanted in syngeneic C57BL/6 mice and from a weakly immunogenic, spontaneously metastasizing line (mFS6) of the same tumor. Macrophages isolated from the FS6 sarcoma non-specifically inhibited growth and DNA synthesis of tumor cells in vitro. After culture FS6 macrophages rapidly lost their cytotoxic activity and became capable of non-specifically stimulating tumor growth in vitro. Macrophages obtained from the mFS6 tumor non-specifically enhanced the proliferative activity of tumor target cells and this capacity was maintained when the macrophages were cultured.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D004273:DNA, Neoplasm; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D012513:Sarcoma, Experimental / Q000276:immunology*; D014185:Transplantation, Isogeneic
D004273:DNA, Neoplasm