title: Lack of gastrointestinal glucagon response to hypoglycaemia in depancreatized dogs.
abstract: Fasting (24 h) normal dogs and depancreatized dogs were injected intravenously with highly purified porcine insulin (Actrapid) in the doses of 0.2 U/kg and 0.5 U/kg, respectively. Blood glucose decreased from 152 +/- 41 (SEM) mg/100 ml to 39 +/- 7 mg/100 ml in the depancreatized dog and from 95 +/- 3 mg/100 ml to 42 +/- 4 mg/100 ml in the normal animal. Using a specific antiserum for "pancreatic" glucagon, the circulating level of glucagon immunoreactivity did not rise from the basal value of 247 +/- 31 pg/ml in the depancreatized group whereas it rose significantly from 223 +/- 24 pg/ml to 321 +/- 41 pg/ml in the normal group. In contrast intravenous infusion of 7 g of arginine increased "pancreatic" glucagon immunoreactivity in both groups. Thus, extrapancreatic glucagon of the pancreatic type does not respond to hypoglycaemia but to arginine infusion.
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism; D004285:Dogs; D005934:Glucagon / Q000276:immunology / Q000378:metabolism*; D004763:Glucagon-Like Peptides / Q000276:immunology / Q000378:metabolism; D007003:Hypoglycemia / Q000378:metabolism*; D010180:Pancreatectomy
chemical_list: D004763:Glucagon-Like Peptides; D005934:Glucagon; D001120:Arginine