[Chitinolytic activity of Actinomyces kurssanovii under periodic and flow cultivation].
Chitinolytic activity of Actinomyces kurssanovii cultivated on the medium containing highly purified crab chitine as carbon source was studied. Under periodic cultivation the first to be accumulated were the (CHI)--enzyme that degraded ground chitine and beta-N-acetylglucosaminidase (EC 3.2.1.30). Then by the 15--16th hour of the producer growth chitinase-poly-(1,4-beta-(2-acetamido-2-deoxy-D-glucoside)-glucanohydrolase (EC 3.2.1.14) and beta-N-acetylglucosaminidase splitting N-N-diacetyl chitobiase were intensively accumulated. It was demonstrated that a highly active chitinolytic preparation of Act. kurssanovii can be obtained under flow cultivation at a dilution coefficient of D = 0.03--0.12 hr-1. A correlation between the respiratory activity of the culture and components of the chitinase system was established.
D000118:Acetylglucosaminidase / Q000096:biosynthesis; D000190:Actinomyces / Q000254:growth & development / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000096:biosynthesis; D003470:Culture Media
D003470:Culture Media; D002686:Chitin; D002688:Chitinases; D000118:Acetylglucosaminidase
Khitinoliticheskaia aktivnost' Actinomyces kurssanovii v usloviiakh pernodicheskogo i protochnogo kul'tivirovaniia.