The use of a measles latex reagent for the determination of measles antibodies and in a specific test for multiple sclerosis.
Measles virus antigens covalently linked to latex spheres were used for measuring measles-specific antibodies in a direct agglutination test either in microtitre plates or as a rapid slide-agglutination test. The titres were compared to that obtained by conventional assays. The measles-latex spheres were also used as the antigen for a radio-immuno assay. By incorporating a 14C-radioactive marker into the measles-latex spheres their interaction with lymphocytes from multiple sclerosis and control patients was determined. Lymphocytes from multiple sclerosis patients reacted with a higher percentage of beads at low bead/lymphocyte ratios compared with controls, whereas the reverse was found when the ratio of beads was increased.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D003937:Diagnosis, Differential; D006801:Humans; D007840:Latex; D007841:Latex Fixation Tests / Q000379:methods; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D008457:Measles / Q000276:immunology*; D008459:Measles virus / Q000276:immunology*; D008863:Microspheres; D009103:Multiple Sclerosis / Q000175:diagnosis*; D011863:Radioimmunoassay
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007840:Latex