title: Identification of lysis protein E of bacteriophage phiX174.
abstract: The product of gene E, the lysis gene of phiX174, has been identified as a distinct band in a sodium dodecyl sulfate-gel electropherogram. The position of the band is consistent with the molecular weight of 10,589 calculated from the nucleotide sequence of the gene. The band is eliminated by a nonsense mutation in gene E. It is estimated that roughly 100 to 300 molecules of E protein are made in an infected cell; this appears to be less than one-tenth the amount of protein made by gene D, in which gene E is wholly contained.
mesh_terms: D003090:Coliphages / Q000032:analysis / Q000235:genetics*; D005814:Genes, Viral; D008242:Lysogeny; D008970:Molecular Weight; D009154:Mutation; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: D014764:Viral Proteins