Low-molecular weight nucleic acids in the blood of rats with Zajdela's hepatoma.
The phenolic method was employed to isolate 2--3 microgram/ml of nucleic acid from rat blood serum. Electrophoresis in an 8% polyacrylamide gel revealed 3 fractions corresponding to 7--9S RNA, and a highly polymer material retained at the beginning of the gel. The blood of the rats with transplanted Zajdela's hepatoma, pre-treated with CCl4, contained an immunodepressive nucleic factor (5S DNA) undetected in healthy animals. This factor may contribute to the suppression of antitumor immunity. The nucleic factor was not detected following treatment with CCl4 alone. It is suggested that the liver hinders nucleic factor transfer from the tumor environs into the blood.
D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000097:blood* / Q000276:immunology; D007165:Immunosuppression Therapy; D008099:Liver / Q000187:drug effects; D008114:Liver Neoplasms, Experimental / Q000097:blood* / Q000276:immunology; D008297:Male; D008970:Molecular Weight; D012334:RNA, Neoplasm / Q000097:blood*; D051381:Rats
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D002251:Carbon Tetrachloride