[Method of isolating, identifying and quantitatively determining alkanes, cycloalkanes, monocyclic arenes and squalene in animal organs and tissues].
A method for analyzing hydrocarbons in animal organs and tissues has been developed. This is made up of the following stages: decomposition of the biological material through alkaline hydrolysis; extraction of non-saponifiable lipids fraction; separation by using preparative thin-layer chromatography of the alkanes and cycloalkanes, monocylic arenes and squalene from the non-saponifiable lipids fractions; determining the content of the alkanes and cycloakanes fraction and also ascertaining the alkanes of normal and isoprenoid structure, by means of gas-liquid chromatography, and, finally, quantification of monocyclic arenes, based on the UV spectra findings. The statistical characteristics obtained through a mathematical processing of the analyses results bear evidence to a sufficient accuracy and good reproducibility of the method stages.
D000473:Alkanes / Q000032:analysis / Q000302:isolation & purification*; D000818:Animals; D003516:Cycloparaffins / Q000032:analysis / Q000302:isolation & purification*; D008722:Methods; D013185:Squalene / Q000032:analysis / Q000302:isolation & purification*
D000473:Alkanes; D003516:Cycloparaffins; D013185:Squalene
Metod vydeleniia, identifikatsii i kolichestvennogo opredeleniia alkanov, tsikloalkanov, monotsiklicheskikh arenov i skvalena v organakh i tkaniakh zhivotnykh.