ATP-independent DNA synthesis in vaccinia-infected L cells.
Mouse L cells can be made permeable to exogenous nucleotides by a cold shock in 0.01 M Tris . HCl pH 7.8, 0.25 M sucrose, 1 mM EDTA, 30 mM 2-mercaptoethanol and 4 mM MgCl2. DNA synthesis in permeabilized L cells requires ATP whereas DNA synthesis in permeabilized L cells that are infected with Vaccinia virus is ATP-independent. Permeabilized L cells that are infected with ultraviolet-irradiated virus show a marked suppression of DNA synthesis which is not corrected by an excess of deoxynucleoside triphosphates and ATP. The ATP-dependent and ATP-independent processes of DNA synthesis are inhibited to the same extent by Mal-Net, pHMB, ara CTP and phosphonoacetate. Concentrations of daunorubicin and cytembena, which cause marked inhibition of the ATP-dependent enzymes, only cause partial inhibition of the ATP-independent enzymes.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001085:Arabinofuranosylcytosine Triphosphate / Q000494:pharmacology; D002463:Cell Membrane Permeability; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis*; D003630:Daunorubicin / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D007739:L Cells; D008623:Mercaptoethanol / Q000494:pharmacology; D010746:Phosphonoacetic Acid / Q000494:pharmacology; D014616:Vaccinia virus / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D001085:Arabinofuranosylcytosine Triphosphate; D008623:Mercaptoethanol; D000255:Adenosine Triphosphate; D010746:Phosphonoacetic Acid; D005033:Ethylmaleimide; D003630:Daunorubicin