Uptake and utilization of 1-14C palmitic acid by heart cells treated with fresh or thermally oxidized fats.
The effects of fractions isolated from thermally oxidized corn oil or olive oil on the metabolic activity of heart endothelial and muscle cells were studied. Rat heart cells in culture, exposed to thermally oxidized fat components, took up more exogenous 1-14C-palmitic acid and incorporated more of it into the cell triacylglycerol fraction than when the cells were treated with fresh fats. Particularly with the heated corn oil compared to fresh corn oil, much less of the radioactivity from the labeled palmitic acid was deposited in the  phospholipid fraction. Also, with heated corn oil when the incubation period was extended beyond 12 hr, there was a decline in the radioactivity retained in the triacylglycerol fraction of the heart muscle cells. When the fresh fats were compared for 14C-radioactivity incorporation into the heart cells, the olive oil gave much higher values, indicating a distinct difference in response to the proportion of fatty acids supplied.
D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport; D002478:Cells, Cultured; D004727:Endothelium / Q000378:metabolism; D007700:Kinetics; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010169:Palmitic Acids / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D051381:Rats; D014280:Triglycerides / Q000096:biosynthesis
D010169:Palmitic Acids; D010743:Phospholipids; D014280:Triglycerides