Glucose transport by horse kidney brush borders. I.--Transport properties of brush border membrane closed vesicles.
Brush border membranes isolated from horse kidney cortex as closed right-side out vesicles show selective permeability when analyzed on sucrose and dextran gradients. These vesicles can actively accumulate D-glucose. The preservation of the glucose transport system is demonstrated by the following features: (a) the uptake and release rates of D-glucose are higher in the presence of a sodium gradient, showing that D-glucose transport is a sodium-dependent process; (b) this transport, specific for the D-isomer, is inhibited by phlorizin; (c) the D-glucose transport system is saturable; (d) no inhibition of D-glucose transport is found with C-mannose; (e) the D-glucose uptake is sensitive to osmotic variations.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002241:Carbohydrates / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D006736:Horses; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism* / Q000648:ultrastructure; D008871:Microvilli / Q000378:metabolism*; D009997:Osmotic Pressure; D010695:Phlorhizin / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology
D002241:Carbohydrates; D012964:Sodium; D010695:Phlorhizin; D005947:Glucose