title: Iron transport by isolated rat intestinal mucosal cells.
abstract: In vitro uptake of radioiron by suspensions of isolated rat duodenal mucosal cells has been examined. The cells bind iron avidly, with uptakes of 15 to 20% after 20 min of incubation. Uptake is dependent upon temperature, pH, and iron and cell concentration. It is unaffected by inhibitors of cellular respiration but is blocked in a concentration-dependent fashion by iron-chelating agents. Iron-deficient cells take up iron at the same rate as normal cells. Binding by brush borders appears to account for 50 to 60% of the total uptake by isolated cells. In vitro radioiron uptake by isolated cells occurs by passive diffusion, is not regulated by the animal's iron status, and is primarily a function of bioavailability of iron in the incubating medium. If the model reflects the behavior of cells in vivo, the initial uptake of iron by the mucosa is a passive process which may serve to concentrate dilute luminal iron at the mucosal surface in preparation for absorption.
mesh_terms: D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001149:Arsenates / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002245:Carbon Dioxide / Q000378:metabolism; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism; D004386:Duodenum; D005293:Ferritins / Q000378:metabolism; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007504:Iron Radioisotopes; D008297:Male; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats
chemical_list: D000963:Antimetabolites; D001149:Arsenates; D002614:Chelating Agents; D007504:Iron Radioisotopes; D002245:Carbon Dioxide; D005293:Ferritins; D003847:Deoxyglucose; D007501:Iron; D011190:Potassium Cyanide