Radioimmunoassay of oxfendazole in bovine, equine, or canine plasma or serum.
A simple radioimmunoassay was developed for the determination of oxfendazole in plasma. Oxfendazole N-1(3)-valerate was coupled to polylysine via a carbodiimide reaction, and antiserum was developed in rabbits after inoculation with oxfendazole--polylysine conjugate. The assay was developed so that oxfendazole could be measured directly in a 0.1-ml aliquot of diluted or undiluted plasma. With the developed procedure, 200 pg of oxfendazole/ml of plasma can be determined quantitatively. Cross-reactivity was determined for closely related compounds and metabolites. The method was used to determine plasma concentration--time profiles in dogs and calves.
D000818:Animals; D000918:Antibody Specificity; D000969:Antinematodal Agents / Q000097:blood*; D001562:Benzimidazoles / Q000097:blood*; D002219:Carbamates / Q000097:blood*; D002417:Cattle; D004285:Dogs; D006736:Horses; D008722:Methods; D010949:Plasma / Q000032:analysis; D011863:Radioimmunoassay
D000969:Antinematodal Agents; D001562:Benzimidazoles; D002219:Carbamates; C011030:oxfendazole