A new RNA-RNA crosslinking reagent and its application to ribosomal 5S RNA.
The synthesis of a new RNA specific bifunctional crosslinking reagent, 1.4-phenyl-diglyoxal, is described which reacts exclusively with guanosines. The properties of the crosslinked products enabled us to develop a straightforward method for identifying the reacted nucleotides. Results obtained with ribosomal 5S RNA of Escherichia coli demonstrate the formation of an intramolecular crosslink between guanosine-2 and guanosine-112 in the stem region.
D000447:Aldehydes / Q000031:analogs & derivatives; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D006037:Glyoxal / Q000031:analogs & derivatives; D006151:Guanosine; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal; D013056:Spectrophotometry, Ultraviolet
D000447:Aldehydes; D012335:RNA, Ribosomal; D006151:Guanosine; D006037:Glyoxal