A modified Marbrook chamber which is cheap and disposable.
The conventional Marbrook culture system has several disadvantages; the preparation and assembly of the chambers is time consuming, the size of the culture vessels limits the number of replicates that may be set up, and placing the cells in the inner chamber is a cumbersome and slow process. We have modified this system by the introduction of a glass ring to make the culture chamber; this is small enough to fit inside a plastic Petri dish which serves as the outer reservoir. The modified Marbrook cultures are easy to manipulate, cheap to produce, disposable, and give comparable results to those obtained with the conventional system.
D000818:Animals; D000917:Antibody Formation; D002478:Cells, Cultured; D004864:Equipment and Supplies; D051379:Mice