An easy and efficient procedure for the isolation of pure DNA restriction fragments from agarose gels.
A new procedure is developed to isolate DNA from agarose gels. Using a kind of blotting technique, DNA is isolated from the gel. It is shown that the isolated DNA can be used for fragmentation by restriction endonucleases, synthesis of complementary RNA by DNA-dependent RNA polymerase from Escherichia coli and nick translation. The procedure gives a high recovery and is easy to perform.
D002021:Buffers; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D006882:Hydroxyapatites
D002021:Buffers; D006882:Hydroxyapatites; D004247:DNA; D004262:DNA Restriction Enzymes