Selected metastable peak monitoring: a new specific technique in quantitative gas chromatography mass spectrometry.
Using the combined electrostatic analyser-magnet scan facility of a double focusing mass spectrometer, the daughter ion peak for the fragmentation, [M-57]+ leads to [M-57-76]+, of a 5alpha-dihydrotestosterone tert-butyl-dimethylsilyl ether was monitored during gas chromatography mass spectrometry. The detection limit was approximately 20 pg. The steroid was detected by this method in an extract of human blood plasma.
D002849:Chromatography, Gas / Q000379:methods*; D013196:Dihydrotestosterone / Q000097:blood; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012825:Silicon
D013196:Dihydrotestosterone; D012825:Silicon