Temperature dependence of sperm-egg fusion and post-fusion events in hamster fertilization.
The effects of temperature (4--37 degrees C) on sperm-egg fusion and the post-fusion events were studied. At 4--10 degrees C, acrosome-reacted spermatozoa bound to egg plasma membranes, but could not fuse with them. At 25 degrees C or above, both binding and fusion took place. The post-fusion events could occur over a broad temperature range (4--37 degrees C) but the events progressed faster with increasing temperature. An abnormal development of egg pronucleus, possibly due to an incomplete functioning of the meiotic spindle mechanism, was observed in eggs inseminated at 37 degrees C and cultured at 25 degrees C.
D000818:Animals; D002459:Cell Fusion; D006224:Cricetinae; D005089:Exocytosis; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D066298:In Vitro Techniques; D008297:Male; D008647:Mesocricetus; D013075:Sperm Capacitation; D013084:Sperm-Ovum Interactions; D013696:Temperature