title: Chronotropic effect of tyramine on rat heart cells cultured with sympathetic neurons.
abstract: Dissociated newborn rat superior cervical ganglion neurons in culture without exogenous nerve growth factor survive and extend processes on a monolayer of rat heart ventricular cells. An increase in the contraction rate of the heart cells was observed in 83% of the co-cultures treated with 5 X 10(-6) M tyramine. No increase was seen in heart cell cultures without neurons. These results are consistent with the assumed mode of action of tyramine--the release of catecholamines from nerve terminals--and suggest that functional interaction can occur in culture between sympathetic neurons and heart ventricular cells.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006339:Heart Rate / Q000187:drug effects*; D009474:Neurons; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D013268:Stimulation, Chemical; D013564:Sympathetic Nervous System / Q000187:drug effects; D014439:Tyramine / Q000494:pharmacology*
chemical_list: D009638:Norepinephrine; D014439:Tyramine