title: Studies on cephalopod rhodopsin. Fatty acid esters of sucrose as effective detergents.
abstract: Squid rhodopsin was extracted with solutions of fatty acid esters of sucrose (monolaurate and monostearate) and purified by DEAE-cellulose and concanavalin A-Sepharose affinity chromatography. The purified rhodopsin (A280/A480 = 2.5) contained 2.3 mol of glucosamine and 1.2 mol of phospholipid per mol of rhodopsin. The photoproduct metarhodopsin was also stable in these detergent solutions as in digitonin solution. Concanavalin A had no affinity for retinochrome.
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D049832:Decapodiformes; D003902:Detergents; D004072:Digitonin; D005944:Glucosamine / Q000032:analysis; D007848:Laurates; D008970:Molecular Weight; D010743:Phospholipids / Q000032:analysis; D012168:Retinal Pigments / Q000302:isolation & purification; D012243:Rhodopsin / Q000302:isolation & purification; D013053:Spectrophotometry; D013228:Stearates; D013395:Sucrose / Q000031:analogs & derivatives*
chemical_list: D003902:Detergents; D007848:Laurates; D010743:Phospholipids; D012168:Retinal Pigments; D013228:Stearates; D013395:Sucrose; D012243:Rhodopsin; D004072:Digitonin; D005944:Glucosamine