GLC determination of nanogram quantities of a new analgesic, nefopam, in human plasma.
A sensitive and specific method was developed for the quantitative GLC determination of plasma nefopam levels. The method includes a multiple-step solvent extraction of the analgesic drug and the internal mass standard, orphenadrine. The accuracy, expressed as the relative error, was--4,6,6, and 4% at 20, 40, 70, and 130 ng/ml, respectively. The precision, expressed as relative standard deviation, was 17, 7, 3, and 5% at these same concentrations, respectively. Quantitation of nefopam in human plasma is possible down to 20 ng/ml with a 2-ml plasma sample; the sensitivity can be increased by using larger plasma samples. The methods was applied successfully to the determination of plasma nefopam levels in humans in pharmacokinetic studies at therapeutic doses.
D002849:Chromatography, Gas; D006801:Humans; D007700:Kinetics; D008722:Methods; D008832:Microchemistry; D009340:Nefopam / Q000097:blood*; D010079:Oxazocines / Q000097:blood*
D010079:Oxazocines; D009340:Nefopam