The first component of complement. A quantitative comparison of its biosynthesis in culture by human epithelial and mesenchymal cells.
Epithelial and mesenchymal cells synthesized and secreted all three subcomponents of the first component of complement (C1): C1q, C1r, and C1s. Quantitatively, however, columnar and transitional epithelial cells secreted 400--3,700 times more hemolytically active C1 than monocytes or fibroblasts. Only columnar epithelial cells synthesized C1 subcomponents with subunit structures similar to their serum counterparts. Transitional epithelial cells, fibroblasts, and monocytes produced C1q and C1s with subunits of apparent molecular weights larger than reported values. C1r from all cell lines was physiochemically similar to serum C1r.
D002478:Cells, Cultured; D003167:Complement Activation; D003172:Complement C1 / Q000096:biosynthesis* / Q000302:isolation & purification; D003513:Cycloheximide / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D008648:Mesoderm / Q000378:metabolism; D008970:Molecular Weight; D009000:Monocytes / Q000378:metabolism
D003172:Complement C1; D046911:Macromolecular Substances; D003513:Cycloheximide