[3H] 2-Amino-6,7-dihydroxy 1,2,3,4-tetrahydronapthalene (ADTN): a potential specific dopamine receptor ligand.
The characteristics of [3H] 2-amino-6,7-dihydroxy 1,2,3,4-tetrahydronapthalene (ADTN) binding to calf striatal membranes were investigated. [3H]ADTN was observed to bind with high affinity and stereospecificity to a preparation of crude synaptic membranes. A number of dopamine agonists and antagonists were studied for their ability to displace bound [3H]ADTN. Dopamine agonists were found to be more potent inhibitors of ADTN binding than were dopamine antagonists. Kinetic analysis indicates that [3H]ADTN binds to dopamine recognition sites in a manner comparable to dopamine itself. Overall, the data suggest that ADTN may be a useful ligand for the characterization of agonist requirements for recognition by dopamine binding sites.
D000818:Animals; D002069:Butaclamol / Q000494:pharmacology; D002417:Cattle; D003342:Corpus Striatum / Q000378:metabolism; D066298:In Vitro Techniques; D008024:Ligands; D008566:Membranes / Q000378:metabolism; D009284:Naphthols / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000187:drug effects / Q000378:metabolism*; D013237:Stereoisomerism; D013764:Tetrahydronaphthalenes / Q000378:metabolism
D008024:Ligands; D009284:Naphthols; D011954:Receptors, Dopamine; D013764:Tetrahydronaphthalenes; C011639:ADTN; D002069:Butaclamol