[Biochemistry and clinical significance of oral exocrine pancreatic function test by means of fluoresceinedilaurate (author's transl)].
Column chromatography of extracts and secretions of the pancreas as well as of sera from patients suffering from inflammations of this organ yields three esterases different from each other in molecular size. These enzymes could be shown to be not identical with lipase. They may be classified as aryl-esterases considering their activities in hydrolyzing synthetic substrates such as esters of fluoresceine with fatty acids. Fluoresceinedilaurate, therefore, proved to be very advantageous in an orally performed test of exocrine pancreatic function. Healthy persons show in this procedure relative excretion of fluoresceine during ten hours of 66.3 +/- 30.4%, patients suffering from pancreatic diseases only 12.7 +/- 9.8% of the dye.
D004950:Esterases / Q000378:metabolism*; D005452:Fluoresceins; D006801:Humans; D008970:Molecular Weight; D010179:Pancreas / Q000201:enzymology*; D010182:Pancreatic Diseases / Q000201:enzymology
D005452:Fluoresceins; D004950:Esterases
Zur Biochemie und klinischen Bedeutung des oralen Pankreasfunktionstests mit Fluoresceindilaurinsäureester.