Partial purification procedure for human urinary erythropoietin by preparative isotachophoresis.
Human urinary erythropoietic-active crude protein was isotachophoretic fractionated in a LKB Uniphor apparatus equipped with a plastic column using polyacrylamide as a stabilizing medium, phosphat ions as leading and glycinat ions as terminating electrolytes and Ampholine carrier ampholytes (pH: 5--7) as spacers. The exhypoxic polycythemic mouse method to assay erythropoietic activity showed that the majority of the hormon was sharply resolved. Under these conditions preparative isotachophoresis demonstrated a higher purification factor compared to other biochemical preparation-methods.
D000741:Anemia, Aplastic / Q000652:urine; D000747:Anemia, Hypochromic / Q000652:urine; D004586:Electrophoresis / Q000379:methods; D004921:Erythropoietin / Q000302:isolation & purification / Q000652:urine*; D006801:Humans
D004921:Erythropoietin