Utilization by the isolated perfused rat liver of N-acetyl-D-[1-14C]galactosamine and N-[3H]acetyl-D-galactosamine for the biosynthesis of glycoproteins.
The isolated perfused rat liver system has been used to monitor the utilization of N-[3H]acetyl-D-galactosamine and N-acetyl-D-[1-14C]galactosamine for the biosynthesis of radiolabelled glycoproteins, which are subsequently secreted into the plasma. Both radiolabels appear in a number of different glycoproteins, predominantly as sialic acid and N-acetylglucosamine. The ratio of labelled sialic acid to labelled N-acetylglucosamine varies for different glycoproteins, but the bulk of N-acetyl-D-galactosamine is incorporated without deacetylation.
D000116:Acetylgalactosamine / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D005260:Female; D005688:Galactosamine / Q000031:analogs & derivatives*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000097:blood; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D014316:Tritium
D002250:Carbon Radioisotopes; D006023:Glycoproteins; D014316:Tritium; D005688:Galactosamine; D000116:Acetylgalactosamine