Isolation and characterization of GD3 ganglioside having a novel disialosyl residue from rabbit thymus.
A major component of the di- and trisialoganglioside fraction of rabbit thymus was isolated. By neuraminidase treatment, methylation analysis, and CrO3 oxidation, it was identified as GD3 ganglioside with the novel disialosyl residue, NeuNGlyc(alpha, 2-8)NeuNAc(alpha, 2-3)Gal-(beta, 1-4)Glc(beta, 1-1)ceramide. Its concentration was 21.92 nmol/g of wet tissue (12.10% of the total gangliosides) and it contained 91.52% of the lipid-bound sialic acid in di- and trisialogangliosides of rabbit thymus.
D000818:Animals; D002554:Cerebrosides / Q000032:analysis; D005732:Gangliosides / Q000302:isolation & purification; D006017:Glycolipids; D011817:Rabbits; D012794:Sialic Acids / Q000032:analysis*; D013950:Thymus Gland / Q000032:analysis*
D002554:Cerebrosides; D005732:Gangliosides; D006017:Glycolipids; D012794:Sialic Acids