Use of logit paper in determination of superoxide dismutase activity in human blood cells.
Superoxide dismutase activity of the human blood cells was determined by the capacity to inhibit nitrite formation from hydroxylammonium chloride. Relationship between percentage of nitrite formation and amount of purified human erythrocyte superoxide dismutase was plotted on conventional graph paper, double log and logit papers. A comparison of the results showed that a linear relationship was obtained only with the logit paper, and thus a more accurate and simple estimation of the activity can be made on a single determination of the specimens.
D000643:Ammonium Chloride / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D008433:Mathematics; D009573:Nitrites / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood*
D009573:Nitrites; D000643:Ammonium Chloride; D013482:Superoxide Dismutase