Schistosoma mansoni: evidence for a role of serum factors in protecting artificially transformed schistosomula against antibody-mediated killing in vitro.
Artificially transformed schistosomula of Schistosoma mansoni develop a consistent but small protection against the lethal effects of antibody plus complement when cultured for 24 h in a chemically defined medium. In contrast, they become rapidly resistant to antibody plus complement, when cultured in the presence of a complex medium consisting of equal parts of heat-inactivated rabbit serum and Earle's/lactalbumin or in defined medium supplemented with small amounts of heat-inactivated rabbit serum. Sephadex G-200 gel filtration revealed that the protective factor in rabbit serum is a macromolecule with a molecular weight between 7 and 19 S. Parasites cultured at 10 degrees C or in the presence of 200 microgram of puromycin show less serum-induced protection against the lethal effects of antibody plus complement than do controls.
D000818:Animals; D000906:Antibodies; D002850:Chromatography, Gel; D003165:Complement System Proteins; D003470:Culture Media; D007106:Immune Sera / Q000032:analysis; D011691:Puromycin / Q000494:pharmacology; D012550:Schistosoma mansoni / Q000187:drug effects / Q000254:growth & development / Q000276:immunology*; D013696:Temperature; D013997:Time Factors
D000906:Antibodies; D003470:Culture Media; D007106:Immune Sera; D011691:Puromycin; D003165:Complement System Proteins