[Preparation of deoxyribonucleic acid (DNA) form salmon milt by the phenol method].
The paper presents a modification of the Kirby method for the DNA preparation from salmon milt. The modified procedure includes ethanol pretreatment and subsequent homogenization in 0.01 M. Trylon B. The paper describes the conditions for DNA purification through alkaline treatment and renaturation at 66 degrees C. The modified method can be used to obtain two preparations of DNA viscous solution in water (DNA homogenate), employed as a raw material in the preparation of 5-deoxyribonucleotides, and a dry preparation.
D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D008297:Male; D008722:Methods; D009695:Nucleic Acid Renaturation; D010636:Phenols; D012474:Salmon; D013094:Spermatozoa / Q000032:analysis*
D010636:Phenols; D004247:DNA
Poluchenie preparatov dezoksiribonukleinovoĭ kisloty (DNK) iz molok lososevykh ryb fenol'nym metodom.