Immobilized-enzyme membrane sandwich reactor used in automated micro-scale assay of plasma uric acid.
We describe the automated microassay of plasma uric acid by use of an immobilized uricase-membrane sandwich reactor. Hydrogen peroxide, formed when uric acid is oxidized, oxidatively couples two molecules of p-hydroxyphenylacetic acid in the presence of peroxidase to produce a highly fluorescent compound. The specificity of the uricase reaction is coupled with a significantly lower cost of analysis.
D001322:Autoanalysis; D004800:Enzymes, Immobilized; D006454:Hemoglobins; D006801:Humans; D008567:Membranes, Artificial; D014503:Urate Oxidase; D014527:Uric Acid / Q000097:blood*
D004800:Enzymes, Immobilized; D006454:Hemoglobins; D008567:Membranes, Artificial; D014527:Uric Acid; D014503:Urate Oxidase