title: In vitro and in vivo effects of exogenous lipids on the enzymatic hydrolysis of rat bile phospholipids.
abstract: The addition of total phospholipids, phosphatidylcholines, triglycerides, cholesterol or glycerol to incubation media containing rat pancreatic juice and bile labeled with [9, 10 3H2] oleic acid (90% of the radioactivity present as phospholipids) had no effect on the hydrolysis of bile endogenous phospholipids. The introduction of 2 or 10 mg of phosphatidylcholines and 0.5 ml of bile (approximately 1.5 mg of phospholipids) into the rat upper duodenum decreased the rate absorption of native bile phospholipids. It was not followed by an increase of free fatty acids released from biliary phospholipids in the intestinal lumen. The introduction of bile (0.5 ml) and small amounts of triolein (1.4--3.5 mg) into the duodenum had little effect on the rate of hydrolysis and absorption of native bile phospholipids, but caused a reduced absorption of the free fatty acids released or those coming from initial nonphosphorus biliary lipids. The introduction of bile (0.5 ml) and large amounts of triolein (30 mg) into the duodenum increased the rates of hydrolysis and absorption of endogenous bile phospholipids. These observations suggest that luminal lipid components can modify the organization of luminal micelles and, consequently, the action of the pancreatic phospholipase A2 and the absorption of bile lipids.
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D008297:Male; D009829:Oleic Acids / Q000378:metabolism; D010189:Pancreatic Juice / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D014304:Triolein / Q000378:metabolism
chemical_list: D009829:Oleic Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D014304:Triolein; D002784:Cholesterol; D005990:Glycerol