Extraction and degradation of timothy pollen allergen during simulated in vivo conditions.
Extraction and degradation of timothy pollen in saline has been compared with extraction in nasal secretion and gastric fluid. By measuring the absolute amount of one important allergenic substance by quantitative immunoelectrophoresis and the total allergenic activity by RAST as a function of time it was shown  that the allergens were extracted extremely fast and that both the total allergenic activity and the concentration of one allergen reached maximum after about 20 min. Compared with saline, extraction under the simulated in vivo conditions gave a lower maximum level and a faster degradation of allergens.
D000485:Allergens / Q000302:isolation & purification*; D001826:Body Fluids / Q000032:analysis; D005750:Gastric Juice / Q000032:analysis; D006801:Humans; D006109:Poaceae; D011058:Pollen / Q000032:analysis*; D011852:Radioallergosorbent Test; D012965:Sodium Chloride / Q000494:pharmacology
D000485:Allergens; D012965:Sodium Chloride