title: Primary structure of human intrinsic factor: progress report on cyanogen bromide fragmentation.
abstract: Human intrinsic factor purified by labile ligand affinity chromatography was cleaved with cyanogen bromide and fractionated by gel filtration. Four of the fragments were purified and sequenced to a total of eighty-four amino acid residues. Including the N-terminal amino acids this amounts to one third of the total amino acid sequence of human intrinsic factor. One of the fragments contained a tyrosine labelled only on iodination of intrinsic factor devoid of cobalamin.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D006801:Humans; D007437:Intrinsic Factor / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D007437:Intrinsic Factor; D003488:Cyanogen Bromide