Partial purification of centrioles from spleen cells.
Isolation of a fraction enriched with centrioles from bovine spleen cells is described. The method involves preparation of a nuclear-centriolar complex and detachment of centrioles from the complex by mechanical treatment in a homogenizer. Purification of centrioles was achieved by centrifugation through a sucrose concentration gradient after treatment by ultrasound, DNase I and Triton X-100. The resulting centrioles retain their in situ ultrastructure.
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002502:Centrioles / Q000648:ultrastructure; D009940:Organoids / Q000648:ultrastructure; D013010:Sonication; D013154:Spleen / Q000648:ultrastructure*; D013347:Subcellular Fractions