Evidence for the incorporation of molecular oxygen, a pathway in biosynthesis of N-glycolylmuramic acid in Mycobacterium phlei.
The hypothesis that the biosynthesis of the glycolyl group of muramic acid in the peptidoglycan of Mycobacterium phlei is catalyzed by a N-acetyl hydroxylase is strongly supported by the experiments reported in this paper. 18O is incorporated into the N-substituent of muramic acid isolated from the peptidoglycan of M. phlei grown under pure oxygen enriched with the 18O isotope.
D002849:Chromatography, Gas; D007553:Isotope Labeling; D009112:Muramic Acids / Q000096:biosynthesis*; D009161:Mycobacterium / Q000378:metabolism*; D009168:Mycobacterium phlei / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010103:Oxygen Isotopes; D013400:Sugar Acids / Q000096:biosynthesis*
D009112:Muramic Acids; D010103:Oxygen Isotopes; D013400:Sugar Acids; D010100:Oxygen