A freeze-etch study of dense fibres in rat spermatozoa.
The presence of oblique striations on the cortex of the dense fibres of the rat spermatozoon is confirmed; they have a main periodicity of approximately 40 nm and a secondary one of about 20 nm. Disruption of spermatozoa by Triton X-100 and dithiothreitol and fixation in glutaraldehyde do not affect the pattern. The dense fibre cortex appears to consist of globular substructure units with a diameter of about 19 nm.
D000818:Animals; D005613:Freeze Etching; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D013094:Spermatozoa / Q000648:ultrastructure*