The development of a fluorometric method for the assay of subtilisins.
An ultra-sensitive fluorometric method has been developed for quantitating subtilisins in aqueous solution. Using detergent solutions as blanks, the analytical sensitivity was estimated to be 0.002 microgram of 100% active enzyme per milliliter. The required sampling time for quantitating subtilisins in air at their TLV was estimated to be approximately two minutes.
D002364:Caseins / Q000031:analogs & derivatives / Q000138:chemical synthesis; D003902:Detergents / Q000032:analysis; D005450:Fluorescamine; D006454:Hemoglobins / Q000138:chemical synthesis; D007202:Indicators and Reagents; D013050:Spectrometry, Fluorescence / Q000379:methods; D013381:Subtilisins / Q000032:analysis*; D013386:Succinates / Q000138:chemical synthesis
D002364:Caseins; D003902:Detergents; D006454:Hemoglobins; D007202:Indicators and Reagents; D013386:Succinates; D005450:Fluorescamine; D013381:Subtilisins