Lack of lysogenic induction in "diaminopimelic acid spheroplasts".
As part of an attempt to develop a semi-in vitro system of lysogenic induction, using spheroplasts of Escherichia coli K-12 lysogenic for prophage lambda, we prepared spheroplasts by depriving E. coli dap of diaminopimelic acid (DAP-spheroplasts). DAP-spheroplasts made from E. coli (lambda cI857) were thermally inducible. However, DAP-spheroplasts of E. coli (lambda) were not inducible by UV light. Thus, it appears that a functional cell wall is required for UV induction of prophage lambda.
D003090:Coliphages / Q000254:growth & development*; D003960:Diaminopimelic Acid / Q000378:metabolism; D006358:Hot Temperature; D008242:Lysogeny; D013104:Spheroplasts / Q000502:physiology*; D014466:Ultraviolet Rays
D003960:Diaminopimelic Acid