Changes in synthesis of specific proteins following axotomy: detection with two-dimensional gel electrophoresis.
Changes in protein synthesis during development and following axotomy were analyzed by two-dimensional gel electrophoresis. The two major postganglionic nerves emerging from the superior cervical sympathetic ganglia (SCSG) of adult rats were either cut or crushed unilaterally. At intervals ranging from 1 to 112 days after surgery both SCSG were removed and incubated for 1 hr in the presence of 14C-leucine. Proteins were extracted and subjected to two-dimensional electrophoretic separation and autoradiography. With this technique, proteins are separated on the basis of isoelectric point and molecular weight. Also, intact SCSG from 1, 2, 7, and 14 day old rats were labeled and analyzed. It was found that a minority of the separated proteins exhibited some detectable change in relative rate of synthesis following axotomy. Actin exhibited a slight (less than 20%) increase in relative synthesis rate while tubulin did not change significantly. There were small but significant differences in the protein patterns following nerve crush, as opposed to nerve cut. Comparison of protein synthesis patterns from developing rat SCSG with those from intact and from axotomized adult SCSG failed to demonstrate any marked similarity between the developmental and the axotomy patterns.
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D001369:Axons / Q000502:physiology*; D004586:Electrophoresis; D005727:Ganglia, Spinal / Q000378:metabolism*; D008297:Male; D009410:Nerve Degeneration; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D051381:Rats; D014404:Tubulin / Q000096:biosynthesis
D000199:Actins; D009419:Nerve Tissue Proteins; D014404:Tubulin