title: A simple, sensitive assay for determining DNA in mononuclear phagocytes and other leukocytes.
abstract: An assay for determining the DNA content of mononuclear phagocytes is described. The assay is efficient and very sensitive, measuring as little as 1 microgram of DNA. Content of DNA is a linear function of the number of mononuclear phagocytes in the sample. One million murine macrophages contain 10.1 +/- 0.36 microgram of DNA. Consequently, samples of as few as 100,000 macrophages can be accurately quantified to be related to other biochemical analyses.
mesh_terms: D004247:DNA / Q000032:analysis*; D008264:Macrophages / Q000032:analysis*; D008722:Methods; D008832:Microchemistry; D009000:Monocytes / Q000032:analysis*; D010586:Phagocytes / Q000032:analysis*
chemical_list: D004247:DNA