An artifact of liquid emulsion autoradiography.
The localization of 3H-opiates in the myenteric plexus of the guinea pig ileum is subject to systematic artifact when stretch preparations of the myenteric plexus are dipped into liquid Kodak NTB-3 emulsion for autoradiography. The cause of the artifact was determined to be a discontinuous distribution, or retraction, of emulsion over plexuses. The apposition of frozen freeze-dried ileal sections to dried photographic emulsion avoids this source of error.
D000818:Animals; D001345:Autoradiography; D004655:Emulsions; D007082:Ileum / Q000032:analysis; D009294:Narcotics / Q000032:analysis; D010781:Photography
D004655:Emulsions; D009294:Narcotics