Insulin activates pyruvate dehydrogenase by lowering the mitochondrial acetyl-CoA/CoA ratio as evidenced by digitonin fractionation of isolated fat cells.
D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D003065:Coenzyme A / Q000378:metabolism*; D004072:Digitonin; D004789:Enzyme Activation / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D051381:Rats
D000227:Adenine Nucleotides; D007328:Insulin; D011768:Pyruvate Dehydrogenase Complex; D000105:Acetyl Coenzyme A; D004072:Digitonin; D003065:Coenzyme A