A perspicuous technique for directly visualizing radiation-damage artefacts in biological electron microscopy.
Levels of impairment of electron-microscopic images of biological specimens stemming from radiation damage are assessed in a rapid visual procedure that involves taking a pair of low-fluence micrographs of a specimen area before and after a fraction of the picture area has been more seriously damaged by applying a measured electron fluence. The pair of micrographs is treated as a mock-stereo pair and is given contrasting colours. Lateral displacements of specimen details appear as false relief and changes in electron lucency as false colour.
D000818:Animals; D002415:Cats; D002540:Cerebral Cortex / Q000528:radiation effects* / Q000648:ultrastructure; D003116:Color; D008854:Microscopy, Electron