[Use of the MUF-5 microspectrophotometer for enzymatic and chromatographic research on a microscale].
The use of MUV-5 as a recording microspectrophotometer for enzymatic and chromatographic analysis on miscroscale is proposed. The tuning of MUV-5 for working with a thermostated microcuvette is described. A microspectrophotometric method of the assay of lactate dehydrogenase activity in a single ovum of the mouse according to the kinetics of enzyme reaction is described.  The proposed method allows to analyse the enzyme quantity 470 times as small as that which can be measured with SPh-16. A method for chromatographic separation of RNA with poly-U-sepharose column is described. The use of MUV-5 for the procedure of chromatographic separation of poly-RNA permits to reduce the amount of RNA in the assayed sample to 50 ng.
D000818:Animals; D002845:Chromatography / Q000295:instrumentation; D004789:Enzyme Activation; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D008832:Microchemistry / Q000295:instrumentation; D010063:Ovum / Q000201:enzymology; D012685:Sepharose; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation*
D012685:Sepharose; D007770:L-Lactate Dehydrogenase
Ispol'zovanie mikrospektrofotometra MUF-5 dlia fermentativnykh i khromatograficheskikh issledovaniĭ v mikroshtabe.