title: Ultrastructural radioautography of the incorporation of tritiated leucine by the rat adrenal medulla in vivo.
abstract: Sections of tissues from the adrenal medullae of young rats were subjected to radioautography after a single intravenous injection of L-leucine 4, 5(3)H to identify the sites of synthesis and follow the migration of newly-formed proteins in both adrenaline-storing (A) and noradrenaline-storing (N) cells. As early as 2 min after injection of leucine 3H, the label was highest in the rough endoplasmic reticulum (RER) of A and N cells, suggesting that cisternal ribosomes are sites of protein synthesis. By 5 and 10 min, much of the label had migrated from the RER into the Golgi complex of both cell types. Some label was already present over the secretory granule matrix (chromogranins) by 2 min but the peak was reached at 1 h in both A and N cells. By 4 h, the label over the secretory granules had diminished, indicating a release of newly-synthetized chromogranins outside the cells. The label over the hyaloplasm was relatively high at 2 min but it decreased rapidly to low levels. In contrast, the label over the cell surface continually increased to reach the highest levels among all organelles at 4 h in both cell types. The pattern of increment of the label over the cell surface suggests that the newly-formed proteins of these sites are also synthetized in the RER, pass through the Golgi complex and are transported in the hyaloplasm, before reaching the surface of A and N cells.
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D001345:Autoradiography / Q000379:methods; D002837:Chromaffin Granules / Q000378:metabolism / Q000648:ultrastructure; D002864:Chromogranins / Q000096:biosynthesis*; D004837:Epinephrine / Q000378:metabolism; D005260:Female; D007930:Leucine; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009638:Norepinephrine / Q000378:metabolism; D009940:Organoids / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats
chemical_list: D002864:Chromogranins; D009419:Nerve Tissue Proteins; D007930:Leucine; D009638:Norepinephrine; D004837:Epinephrine