[Comparative study of six methods for lymphocyte isolation from several mammalian sources and determination of their carbohydrate composition (author's transl)].
The present paper deals with a comparative study on six methods for isolation of peripheral blood lymphocytes from various mammalian sources: Bos taurus L. (adult cow), Equus caballus L. (adult horse), Equus asinus L. (adult and young donkeys) and Sus scropha L. (adult pig). The following systems were used: a) Filtration through sand columns (a modification of Blaszczyszyn's method); b) Sodium metrizoate and "Ficoll 400" c) "Lymphoprep"; d) "Urovison" and dextran T150 (a modification of GILI et al.'s method); e) "Urografin" and dextran T150; f) "Ficoll-Paque". The final preparation of lymphocytes obtained by "Urovison" and dextran T150 (d = 1.081) procedure was free from platelets and erythrocytes; lymphocytes degree of purity was found to be 98%. The sialic acids, hexoses and hexosamines contents were determined.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002417:Cattle; D002469:Cell Separation / Q000379:methods*; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006654:Histology, Comparative; D006736:Horses; D008214:Lymphocytes / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis; D013552:Swine
D002241:Carbohydrates; D006595:Hexosamines; D006601:Hexoses; D012794:Sialic Acids
Estudio comparativo sobre seis procedimientos de aislamiento de linfocitos de mamíferos y determinación de su composición glucídica.