Electrophoretic variation of enzymes: a further marker for genetic studies of the Eimeria.
Embryo-adapted strains of Eimeria mivati and E. mivati var. diminuta, differing in their sensitivity to sulphaquinoxaline and electrophoretic mobilities of lactate dehydrogenase, were crossed. E. mivati was sulphaquinoxaline-resistant and characterised by an electrophoretic form of the enzyme denoted lactate dehydrogenase-1 whereas E. mivati var. diminuta was sulphaquinoxaline-sensitive and characterised by lactate dehydrogenase-6. Progeny recovered from the cross were passaged in embryonating eggs given sulphaquinoxaline and the (drug-resistant) parasites recovered were characterised by both lactate dehydrogenase-1 and lactate dehydrogenase-6. Controls showed that those parasites characterised by the recombinant phenotype of drug-resistant and lactate dehydrogenase-6 had been produced by the cross-fertilisation of gametes.
D000818:Animals; D002642:Chick Embryo / Q000469:parasitology; D004352:Drug Resistance, Microbial; D004539:Eimeria / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D011995:Recombination, Genetic; D013170:Spores; D013428:Sulfaquinoxaline / Q000494:pharmacology
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D013428:Sulfaquinoxaline