title: Continuous-flow analysis for serum albumin, by use of the immediate reaction with bromcresol green.
abstract: Albumin reacts with bromcresol green reagent almost immediately, other serum proteins more slowly. This phenomenon can be exploited to improve the specificity of this routine method for determining serum albumin. I describe a 30-s reaction as adapted to continuous-flow analyzers, with no interference from other serum proteins. The method shows linearity to 72 g/liter. Results of this method correlate very well with those by the Laurell "rocket" and manual (30-s) bromcresol green methods. This procedure gives a considerable improvement in specificity for continuous-flow analyzers as compared with current bromcresol green methods.
mesh_terms: D001322:Autoanalysis / Q000295:instrumentation; D001961:Bromcresol Green; D003408:Cresols; D006801:Humans; D008722:Methods; D012709:Serum Albumin / Q000032:analysis*
chemical_list: D003408:Cresols; D012709:Serum Albumin; D001961:Bromcresol Green