The primary structure of tubulin. Sequences of the carboxyl terminus and seven other cyanogen bromide peptides from the alpha-chain.
The alpha-chain of calf brain tubulin was fragmented by treatment with cyanogen bromide and eight peptides together accounting for 108 residues were purified and sequenced. The COOH-terminal peptide contains a fractional amount (about 0.3 residues) of tyrosine at its COOH-terminus; this presumably represents tyrosine that is added post-translationally to alpha-tubulin. The beta-chain can be phosphorylated, and the probable site of this modification is identified also in the COOH-terminal peptide. Comparison of the sequences described here with the sequence of actin reveals no homology between actin and tubulin.
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D003488:Cyanogen Bromide; D006023:Glycoproteins; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases; D014404:Tubulin
D006023:Glycoproteins; D010446:Peptide Fragments; D014404:Tubulin; D010447:Peptide Hydrolases; D003488:Cyanogen Bromide