title: Phosphorylase kinase isoenzymes in deficient ICR/IAn mice.
abstract: ICR/IAn mice present a deficiency in phosphorylase kinase activity; the extent of this deficiency is less in some tissues [Lyon, S.B. Biochem. Genet. 4, 169--185 (1970)] than in skeletal muscle, where enzyme activity is 0.3% of normal [Cohen, P.T. W & Cohen, P. FEBS Lett. 29, 113--115 (1973)].  New-born mice of this strain were also reported (Lyon, 1970) to reveal a small amount of skeletal muscle enzyme activity. The properties of these residual phosphorylase kinases were compared to those of control C57 BL mice, with reference to control muscle and liver enzymes which were shown to be of different molecular species [Daegelen-Proux et al. Biochim. Biophys Acta, 452, 398--405 (1976)]. The properties investigated were the immunological reactivity against an antiserum raised against muscle phosphorylase kinase, the thermal stability and the Ca2+ dependency. The results suggest that the muscle enzyme from the new-born ICR/IAn mice and the heart enzyme from adult deficient mice are different to the muscle enzyme from adult normal mice, but they have properties in common with normal adult liver enzyme. These results lead to the conclusion that there exists in the muscle of I strain a "foetal form" of phosphorylase kinase, the activity of which decreases progressively after birth. Out work also confirmed the observations made by Cohen et al. [Eur. J. Biochem. 66, 347--356 (1976)] which showed that there is no evidence for the existence of a cross-reacting material in the muscle of adult deficient mice.
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000494:pharmacology; D003429:Cross Reactions; D004355:Drug Stability; D007527:Isoenzymes / Q000172:deficiency* / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D024510:Muscle Development; D009132:Muscles / Q000201:enzymology; D010764:Phosphorylase Kinase / Q000172:deficiency* / Q000378:metabolism; D011233:Precipitin Tests
chemical_list: D007527:Isoenzymes; D010764:Phosphorylase Kinase; D002118:Calcium