[Replication and reassociation kinetics of nuclear matrix DNA from regenerating rat liver].
Fraction 1, containing 65--70% of nuclear DNA (nDNA) was extracted from isolated rat liver nuclei. Then nuclear matrix fraction, containing 30--35% of nDNA was extracted with 1.2 M NaCl. About 1% of nDNA war discovered in residual matrix. Specific activity of residual matrix labelled DNA within 1 and 3 min. after the injection of 14C-orotic acid into the liver portal vein (24 hours after hepatectomy) was respectively in 70 and 50 times higher, and that of matrix DNA--in 45 and 20 times as high as in fraction 1 DNA. Thus, replication begins from nuclear matrix DNA. The latter is enriched with unique sequences by 10%, as compared with total nDNA, and its reassociation kinetics does not change at different stages of the cell cycle (0 and 24 hours after partial hepatectomy). It is suggested, that DNA does not migrate with respect to nuclear matrix under replication.
D000818:Animals; D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism*; D004261:DNA Replication; D006498:Hepatectomy; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration; D009695:Nucleic Acid Renaturation; D009963:Orotic Acid / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D009963:Orotic Acid
Replikatsiia i kinetika reassotsiatsii DNK iadenogo matriksa Regeneriruiushchei pecheni krysy.