Isolation of a growth-stimulating agent from human skin fibroblast cultures.
Cell-free supernatants were harvested from cultures of human skin fibroblasts, were applied on to DEAE-cellulose columns, and the first fraction eluted with phosphate-buffered saline contained the growth-stimulating agent. The eluted fraction was then passed through a series of amicon membranes. After passing through PM-10, the filtrate stimulated growth of bovine vascular endothelial, canine myocardial, and human mammary carcinoma cells.
D001808:Blood Vessels; D001943:Breast Neoplasms; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004727:Endothelium; D005347:Fibroblasts / Q000032:analysis*; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology; D006352:Heart Ventricles; D006801:Humans
D006133:Growth Substances