[Mechanisms of contraction and relaxation in the response to 5'hydroxytriptamine of isolated rat ileum (author's transl)].
The action of 5-hydroxytryptamine (5-HT) in the rat ileum was analysed pharmacologically. In the isolated rat ileum, either mono or biphasic contraction was induced depending on the concentrations of 5-HT. The former was induced by lower concentrations of 5-HT (less than 6.25 X 10(-7)M) and the latter by higher concentrations of 5-HT (greater than 2.5 X 10 (-6)M). The monophasic contraction and the tonic contraction in response to 5-HT were blocked by methysergide (MTG). The phasic contraction in response to 5-HT after inhibition of muscular receptor with MTG was potentiated by physostigmine and blocked by mecamylamine (MC) or tetrodo-toxin (TTX). The response to 5-HT changed from a contraction to a relaxation by preincubation with MTG plus hyoscine. The relaxation was inhibited by TTX but not by MC and such was provoked even in the ileum from rats treated with 6-hydroxydopamine. Therefore, the inhibitory neuronal receptor for 5-HT does not appear to belong to the adrenergic nervous system. At lower concentrations of 5-HT, relaxation was induced with MTG which was only partially inhibited by TTX. These results indicate that the stimulatory response to 5-HT were caused by activations of muscular receptors and of intramural parasympathetic ganglion cells while inhibitory responses were caused by activations of non-adrenergic inhibitory nerve terminals and of non-neuronal elements.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006892:Hydroxydopamines / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D008464:Mecamylamine / Q000494:pharmacology; D008784:Methysergide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D009020:Morphine / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects*; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D010830:Physostigmine / Q000494:pharmacology; D051381:Rats; D011955:Receptors, Drug; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists; D013779:Tetrodotoxin / Q000494:pharmacology
D006892:Hydroxydopamines; D011955:Receptors, Drug; D012702:Serotonin Antagonists; D012701:Serotonin; D013779:Tetrodotoxin; D008464:Mecamylamine; D009020:Morphine; D010830:Physostigmine; D008784:Methysergide