An experimental model of osteogenesis: a closed in vivo culture system using diffusion chambers.
Part of young mouse radii were isolated and transplanted into an intraperitoneal Millipore diffusion chamber. The survival of the implants was followed for 22 days, using morphological criteria, and by autoradiography with tritiated thymidine. Periosteal cells and epiphyseal plate chondrocytes survived well for one to two weeks. DNA synthesis was noted up to the twenty-second day of culture. Osteogenetic cells proliferated well in transplants of shorter duration, but in the third week there was overgrowth by fibroblasts. The method seems well suited for the culture of osteogenetic cells as whole-bone pieces for at least one to two weeks.
D000818:Animals; D001345:Autoradiography; D001842:Bone and Bones / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005260:Female; D008297:Male; D051379:Mice; D008954:Models, Biological; D010012:Osteogenesis; D011884:Radius / Q000166:cytology
D004247:DNA