A simple, sensitive method for the quantitation of kanamycin in tissue and biological fluids.
A method is described for the quantitative determination of kanamycin in blood, urine, cochlear perilymph and kidney. The method is simple, involving only protein precipitation, chromatographic separation and fluorometric determination following reaction with fluorescamine. The method is sensitive, determining as little as 80--100 ng/ml of kanamycin. The method is reliable, recovering better than 90% of the kanamycin added to the fluids and tissues mentioned. It is also less cumbersome and less expensive than other methods currently used.
D000818:Animals; D001826:Body Fluids / Q000032:analysis; D002852:Chromatography, Ion Exchange; D005450:Fluorescamine; D005470:Fluorometry; D006168:Guinea Pigs; D007612:Kanamycin / Q000032:analysis* / Q000097:blood / Q000652:urine; D007668:Kidney / Q000032:analysis; D008722:Methods; D010498:Perilymph / Q000032:analysis
D005450:Fluorescamine; D007612:Kanamycin