Demonstration of the presence of M-creatine kinase in mammalian myogenic cell lines.
Unequivocal identification of M-CK in cell extracts from fused cells of myogenic cell lines is difficult due to almost identical behaviour of the M-CK and a contaminating enzyme activity in electrophoresis. If CK dimers present in cell extracts were subjected to dissociation and reassociation in the presence of exogenous B-CK subunits, the formation of easily identifiable MB-CK was demonstrated, indicating the presence of M-CK in the myogenic rat cell lines.
D002454:Cell Differentiation; D002459:Cell Fusion; D002460:Cell Line; D003402:Creatine Kinase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000201:enzymology*
D007527:Isoenzymes; D003402:Creatine Kinase