[Mechanism of methylation of DNA bases by symmetrical dimethylhydrazine].
The effect of disulfiram on the alkylation of purine bases of the liver and colon DNA in rats exposed to 1,2-dimethyl-hydrazine-3H has been studied. The pretreatment of rats with disulfiram which inhibits the DMH N-oxydation was found to prevent the methylation of guanine with DMH. However, the radioactivity was readily incorporated into normal (non-methylated) purine bases during synthesis. The lack of DNA methylation in animals pretreated with disulfiram indicated carbonium ion rather than methyl radical to alkylate DNA.
D000818:Animals; D004247:DNA / Q000378:metabolism*; D004127:Dimethylhydrazines / Q000494:pharmacology*; D004221:Disulfiram / Q000494:pharmacology; D004347:Drug Interactions; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008745:Methylation; D008763:Methylhydrazines / Q000494:pharmacology*; D051381:Rats
D004127:Dimethylhydrazines; D008763:Methylhydrazines; D004247:DNA; D004221:Disulfiram
Mekhanizm metilirovaniia osnovaniĭ DNK simmetrichnym dimetilgidrazinom.