Angiotensin stimulates cortisol biosynthesis in human adrenal cells in vitro.
Adrenal glands obtained from patients undergoing therapeutic adrenalectomy were used to study the effects of angiotensin on human adrenal steroidogenesis. It was observed that angiotensin stimulated cortisol biosynthesis. Although this has been demonstrated to occur in canine and bovine adrenals, angiotens in-induced cortisol biosynthesis has not been established in man. The possibility that angiotensin merely stimulated glomerulosa cells to secrete precursor steroids which accumulated in the medium and then diffused into fasciculata cells to provide substrate for cortisol biosynthesis was excluded by demonstrating that 3beta-hydroxy-5-pregnen-20-one (pregnenolone) and progesterone (the only pertinent precursors) did not accumulate in angiotensin-stimulated cell suspension. In addition, angiotensin stimulated cortisol biosynthesis in a fasciculata cell suspension in which angiotensin did not stimulate aldosterone production. Therefore, in human adrenal cell suspensions angiotensin appeared to act directly to stimulate cortisol synthesis by fasciculata cells. In normal subjects pre-treated with dexamethasone, angiotensin infusions failed to stimulate an increase in plasma cortisol. The physiological importance of angiotensin as a regulator of cortisol secretion remains, therefore, to be established.
D000311:Adrenal Glands / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000450:Aldosterone / Q000096:biosynthesis; D000804:Angiotensin II / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D004347:Drug Interactions; D006801:Humans; D006854:Hydrocortisone / Q000096:biosynthesis* / Q000097:blood; D066298:In Vitro Techniques; D011284:Pregnenolone / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism
D000804:Angiotensin II; D000450:Aldosterone; D011374:Progesterone; D011284:Pregnenolone; D003907:Dexamethasone; D006854:Hydrocortisone