Simultaneous programmed temperature GLC assay of phenol, chloroxylenol, and lidocaine hydrochloride in topical antiseptic cream.
A simultaneous programmed temperature GLC assay for the active ingredients in a topical antiseptic cream is described. The sample is extracted directly using a dimethyl sulfoxide solution of p-cresol, 4-chlorophenol, and 2-amino-4-phenylthiazole as internal standards for phenol, chloroxylenol, and lidocaine hydrochloride, respectively. The resulting solution is chromatographed by temperature programming on an OV-225 column from 90 to 225 degrees. The internal standard calculation is accomplished using peak heights or peak areas. The relative standard deviation of all assays is less than 2%.
D000891:Anti-Infective Agents, Local / Q000032:analysis*; D002849:Chromatography, Gas; D004338:Drug Combinations; D008012:Lidocaine / Q000032:analysis*; D008722:Methods; D009824:Ointments; D010636:Phenols / Q000032:analysis*; D013696:Temperature; D014992:Xylenes / Q000032:analysis*
D000891:Anti-Infective Agents, Local; D004338:Drug Combinations; D009824:Ointments; D010636:Phenols; D014992:Xylenes; C007027:chloroxylenol; D008012:Lidocaine