Isolation of human urinary lysozyme.
For the isolation of human lysozyme from the urine of leukemia patients, a simple method has been established which involves precipitation of urinary proteins by 60% saturation with ammonium sulfate, fractionation of crude lysozyme on Sephadex G-50 and purification by CM-Sepharose chromatography. By this method approximately 60% of the lysozyme in the urine was isolated in a pure state in ten days.
D000596:Amino Acids / Q000032:analysis; D006801:Humans; D007700:Kinetics; D007938:Leukemia / Q000201:enzymology; D009113:Muramidase / Q000302:isolation & purification / Q000652:urine*
D000596:Amino Acids; D009113:Muramidase