[RNA synthesis in the isolated nuclei of nerve cells treated with acetylcholine].
Acetylcholine modified the incorporation of radioactive precursors 3H-uridine-5-triphosphate, 14C-uridine and 14C-cytidine in RNA of nuclei isolated from the rat brain. This modification has two tendencies: either the activation or the suppretion. The character of the nuclear reaction depends upon the season and animal population. In the presence of actinomycin D (5.10(-5) M), acetylcholine does not influence the precursor incorporation into the nuclear RNA. In the system that involves nuclei+soluble cytoplasmic fraction acetylcholine exerts an effect that is opposite to that when the nuclei are alone. These results suggest that acetylcholine action is mediated by a cytoplasmic factor. Actinomycin D in the presence of cytoplasmic fraction did not influence effects of acetylcholine.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D005260:Female; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D012621:Seasons
D003609:Dactinomycin; D012313:RNA; D000109:Acetylcholine
Sintez RNK v izolirovannykh iadrakh nervnykh kletok pri vozdeĭstvii atsetilkholina.