Increased uptake and desulphation of heparin by mouse macrophages in the presence of polycations.
Heparin uptake and desulphation by cultured macrophages were investigated. Histones, polyamino-acids, protamine and eosinophil-basic protein stimulated both heparin uptake and desulphation, processes found to be non-related. Poly-L-ornithine and poly-DL-lysine increased the heparin uptake by about 33-fold, and histone produced up to 7.5-fold increase in the desulphation. The same polycations inhibited heparin desulphation by macrophage extracts.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001798:Blood Proteins / Q000494:pharmacology; D002478:Cells, Cultured; D004804:Eosinophils; D006493:Heparin / Q000378:metabolism*; D006657:Histones / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D010455:Peptides / Q000494:pharmacology; D011479:Protamines / Q000494:pharmacology; D013464:Sulfuric Acids / Q000378:metabolism
D001798:Blood Proteins; D006657:Histones; D010455:Peptides; D011479:Protamines; D013464:Sulfuric Acids; D006493:Heparin