Continuous-flow analysis for serum albumin, by use of the immediate reaction with bromcresol green.
Albumin reacts with bromcresol green reagent almost immediately, other serum proteins more slowly. This phenomenon can be exploited to improve the specificity of this routine method for determining serum albumin. I describe a 30-s reaction as adapted to continuous-flow analyzers, with no interference from other serum proteins. The method shows linearity to 72 g/liter. Results of this method correlate very well with those by the Laurell "rocket" and manual (30-s) bromcresol green methods. This procedure gives a considerable improvement in specificity for continuous-flow analyzers as compared with current bromcresol green methods.
D001322:Autoanalysis / Q000295:instrumentation; D001961:Bromcresol Green; D003408:Cresols; D006801:Humans; D008722:Methods; D012709:Serum Albumin / Q000032:analysis*
D003408:Cresols; D012709:Serum Albumin; D001961:Bromcresol Green