Effects of aminooxyacetate, alanine and other amino acids on protein synthesis in isolated rat hepatocytes.
Protein synthesis in isolated rat hepatocytes has been measured by the incorporation of [14C]valine at high concentration and constant specific activity (5 mmol/l and 315 muCi/l). Protein synthesis was stimulated by the addition of an amino acid mixture, and by a number of individual amino acids alone, most notably alanine. Energy substrates (lactate, pyruvate) stimulated protein synthesis to the same extent as alanine, suggesting that a major part of the amino acid effect could be due to the provision of energy. Aminooxyacetate, an inhibitor of glutamate transaminases, inhibited protein synthesis strongly (70%), and abolished the stimulatory effects of alanine and energy substrates. This could indicate that hepatocytic protein synthesis is subject to positive control by a transamination-dependent agent.
D000596:Amino Acids / Q000494:pharmacology*; D000625:Aminooxyacetic Acid / Q000494:pharmacology; D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats; D014633:Valine / Q000378:metabolism
D000596:Amino Acids; D000625:Aminooxyacetic Acid; D000641:Ammonia; D014633:Valine