Middle-sized ninhydrin-positive molecules in uraemic patients treated by repeated haemodialysis. II. Chief peptide constituents of the faction.
The fraction of middle-sized molecules obtained from haemodialysate by chromatography on Dowex 50W x 8 resolves upon thin-layer electrophoresis on Sephadex G-25 into nine ninhydrin-positive fractions and one staining only with tolidine reagent. All ten electrophoretic fractions give similar filtration profiles with Bio-Gel P-6 in the presence of SDS and 2-mercaptoethanol. The chief peptide components common to each electrophoretic fraction have molecular weight approx. 3000 and are especially abundant in lysine, glycine, glutamine acid and serine, while aromatic amino acids are in very low concentration.
D000596:Amino Acids / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D009555:Ninhydrin; D010455:Peptides / Q000097:blood*; D006435:Renal Dialysis; D014511:Uremia / Q000097:blood*
D000596:Amino Acids; D010455:Peptides; D009555:Ninhydrin