Measurement of oestradiol receptors by five institutions on common tissue samples.
The soluble oestrogen-receptor content of common breast tumours has been measured by 5 different laboratories, each using their own assay procedure. Good agreement was achieved on whether a sample was positive or negative for oestrogen receptor. Qualitative differences between laboratories could be explained by differences in thiol-reagent content of assay medium and by the method of homogenization. Recommendations are made on some of the factors involved in the routine assay of receptors in breast tumours.
D001943:Breast Neoplasms / Q000032:analysis*; D004958:Estradiol; D005260:Female; D006801:Humans; D008722:Methods; D011960:Receptors, Estrogen / Q000032:analysis*; D013438:Sulfhydryl Compounds; D014021:Tissue Preservation; D006113:United Kingdom
D011960:Receptors, Estrogen; D013438:Sulfhydryl Compounds; D004958:Estradiol