Interference by branched-chain amino acids in the assay of alanine with alanine dehydrogenase.
Commercial preparations of alanine dehydrogenase from Bacillus subtilis are contaminated to varying extents with activity towards branched-chain amino acids. The Km values for these amino acids are of the same order as for L-alanine (about 10(-3)M). The branched-chain amino acid dehydrogenase activity is lost on dialysis for 2--4h against water or 2mM-EDTA.
D000409:Alanine / Q000032:analysis*; D000594:Amino Acid Oxidoreductases; D000597:Amino Acids, Branched-Chain; D007651:Keto Acids / Q000032:analysis; D007700:Kinetics
D000597:Amino Acids, Branched-Chain; D007651:Keto Acids; D000594:Amino Acid Oxidoreductases; D000409:Alanine