Physical studies of nucleosome assemble.
Biochemical, spectroscopic, and hydrodynamic studies were performed on the reconstituted complex of 140 base pair DNA and the arginine-rich histone tetramer (H3/H4)2. The histones bind to DNA in a 1:1 molar ration to form a stable particle which orients in an electric field with a rotational correlation time of 6.3 mus and a limitign reduced dichroism of --0.74. The complex was modeled hydrodynamically as a cylinder of dimensions 450 X 80 X 80 A containing approximately 1.5 superhelical turns. Addition of the lysinerich histones to this complex cause a condensation of the structure and results in physical properties nearly identical with those of a native nucleosomal particle.
D000818:Animals; D001120:Arginine; D002417:Cattle; D002467:Cell Nucleus / Q000648:ultrastructure*; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA; D004563:Electrochemistry; D006657:Histones; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D013057:Spectrum Analysis; D013950:Thymus Gland
D002843:Chromatin; D006657:Histones; D004247:DNA; D001120:Arginine