Laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613.
Conditions for the laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613 were studied. Thirty-five g acetoin plus diacetyl/50 g sucrose were obtained when fermentation was carried out in 2.5 liter medium containing 12.5 g peptone and 12.5 g yeast extract, at pH 7.0, in a 5 liter conical flask on a shaker (240 rpm) at 28-30 degrees C for 48 hr. Recovery of pure diacetyl was 85% of the total acetoin plus diacetyl.
D000093:Acetoin / Q000096:biosynthesis*; D002074:Butanones / Q000096:biosynthesis* / Q000378:metabolism*; D002241:Carbohydrates / Q000494:pharmacology; D003470:Culture Media; D003931:Diacetyl / Q000378:metabolism*; D004754:Enterobacter / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000378:metabolism*; D005285:Fermentation; D008965:Molasses; D013395:Sucrose / Q000378:metabolism
D002074:Butanones; D002241:Carbohydrates; D003470:Culture Media; D013395:Sucrose; D000093:Acetoin; D003931:Diacetyl