[Studies on the excitation-metabolism coupling mechanism in brown adipose tissues, 2; Effects of K+ and norepinephrine on the lipolysis in brown adipose tissues (author's transl)].
This investigation was undertaken to clarify the lipolytic response caused by K+ and norepinephrine in brown adipose tissues. 1. Lipolytic response was obviously observed at the concentration of 50 mM in the K+-induced stimulation, and at 1.0 microgram/ml in the norepinephrine-induced stimulation, respectively. 2. K+- and norepinephrine-stimulated lipolysis were inhibited in the Ca2+-deficient tissues, and were inhibited by the addition of Mg2+, Mn2+ and La3+. 3. In the K+-stimulated lipolysis Ca2+ could be substituted only by Sr2+, while in the norepinephrine-stimulated lipolysis the substitution was possible with both Sr2+ and Ba2+. 4. K+- and norepinephrine-stimulated lipolysis were inhibited by the addition of propranolol and procaine. Accordingly, these positive results suggest that K+- and norepinephrine-stimulated lipolysis are dependent on the presence of Ca2+, and are related to the movements of Ca2+ and cyclic AMP system in the cells.
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000502:physiology; D002413:Cations, Divalent; D005990:Glycerol / Q000378:metabolism; D066298:In Vitro Techniques; D007811:Lanthanum / Q000494:pharmacology; D050356:Lipid Metabolism; D008297:Male; D009638:Norepinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D011188:Potassium / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011343:Procaine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats
D002413:Cations, Divalent; D011343:Procaine; D007811:Lanthanum; D011433:Propranolol; D005990:Glycerol; D011188:Potassium; D002118:Calcium; D009638:Norepinephrine