Simultaneous determination of retinol and retinyl esters in serum or plasma by reversed-phase high-performance liquid chromatography.
We propose a single-run liquid-chromatographic determination, with ultraviolet detection at 330 nm, for serum retinol and retinyl esters. The vitamin A derivatives are extracted according to the Bligh-Dyer procedure. With 200 microliter or serum, the lower detection limit is 50 microgram/liter for retinol and about 100 microgram/liter for retinyl esters. Within-run precision (CV) was 2.3% for retinol, 4.3% for retinyl palmitate. Day-to-day percision (CV, n = 20) for retinol was 4.9% during a month. The method can be used for the assessment of vitamin A absorption tests and for the determination of serum retinol (normal, subnormal, and above-normal concentrations). Serum retinyl esters can only be measured in conditions where concentrations exceed 100 microgram/liter.
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D004952:Esters; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D010168:Palmitates / Q000097:blood; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000097:blood*
D004952:Esters; D010168:Palmitates; D014801:Vitamin A