DNA ligase activity in UV-irradiated monkey kidney cells.
The DNA ligase activity of monkey kidney CV-1 cells has been measured at different stages of culture growth and after different time intervals following ultraviolet irradiation. Results indicate that: - The level of enzyme activity is about twice higher in non synchronous, rapidly dividing cells than in confluent cultures. - UV-irradiation of cells induces a "de novo" synthesis of DNA ligase. - This induction is dose dependent in its extent and kinetics, and may lead to a DNA ligase level in UV-irradiated stationary cultures of the same order as observed in unirradiated exponentially growing cells. - This induction seems to be independent of semiconservative DNA synthesis since it is not affected by fluorodeoxyuridine.
D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D011088:DNA Ligases / Q000378:metabolism / Q000528:radiation effects*; D004790:Enzyme Induction / Q000528:radiation effects; D007700:Kinetics; D011118:Polynucleotide Ligases / Q000528:radiation effects*; D014466:Ultraviolet Rays
D011088:DNA Ligases; D011118:Polynucleotide Ligases