[Enzymatic determination of xylitol and sorbitol (author's transl)].
An enzymatic endpoint determination of xylitol and sorbitol by the use of L-iditol dehydrogenase is described. Despite the use of pH 9 and the addition of semicarbazide, the reaction goes only 80% to completion. Therefore a standard value has to be used. The lower limit for measurement of concentrations in serum is around 0.1 mmol/l. The most favorable range is between 0.4 and 1.2 mmol/l with a coefficient of variation of below 4%. Sensitivity and precision is higher than with the kinetic method described earlier. Further simplification for the routine control of infusion therapy with polyols is suggested.
D006801:Humans; D007700:Kinetics; D008722:Methods; D010088:Oxidoreductases; D010783:Photometry; D013012:Sorbitol / Q000097:blood*; D014993:Xylitol / Q000097:blood*
D013012:Sorbitol; D010088:Oxidoreductases; D014993:Xylitol
Enzymatische Bestimmung von Xylit und Sorbit nach dem Endwertverfahren.