Rapid method for identification and enumeration of oral Actinomyces.
Serotype-specific antisera prepared against whole cells of Actinomyces viscosus, A. naeslundii, and A. israeli were labeled with fluorescein dye and used to detect and quantitate antigenically related microorganisms in human dental plaque. By relating the DNA content of the dental plaque microflora to the number of Actinomyces present in the plaque samples, a reproducible method was developed for specifically enumerating five serotypic representatives of this genus found in human plaque.
D000190:Actinomyces / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D000918:Antibody Specificity; D003773:Dental Plaque / Q000382:microbiology*; D006801:Humans; D012703:Serotyping