title: Subcellular localisation and identification of superoxide dismutase in the leaves of higher plants.
abstract: 1. The subcellular location of superoxide dismutase in the leaves of spinach and other C3 plants has been investigated. 2. Most activity appeared to be located within chloroplasts. These organelles contain a cyanide-sensitive (copper-zinc) superoxide dismutase, most of which is located in the stroma although some is bound to the thylakoids. 3. Intact chloroplast fractions also contain a cyanide-insensitive (manganese) superoxide dismutase, but this activity is located on the outside of the chloroplasts and may be adsorbed onto them during isolation. 4. Leaf mitochondrial fractions contain only a small percentage of total leaf superoxide dismutase activity, but there is more than can be accounted for by contamination with chloroplasts. 5. Mitochondria contain both a cyanide-sensitive dismutase, apparently located in the intermembrane space, and a cyanide-insensitive activity, apparently located in the matrix. 6. The microsomal fraction contains no superoxide dismutase activity.
mesh_terms: D002736:Chloroplasts / Q000201:enzymology; D003300:Copper; D003486:Cyanides / Q000494:pharmacology; D008345:Manganese; D008928:Mitochondria / Q000201:enzymology; D010944:Plants / Q000201:enzymology*; D013045:Species Specificity; D013347:Subcellular Fractions / Q000201:enzymology; D013482:Superoxide Dismutase / Q000302:isolation & purification / Q000378:metabolism*; D015032:Zinc
chemical_list: D003486:Cyanides; D008345:Manganese; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc