125I-labeled cell surface as a marker in preparation of microsome fraction by gel filtration.
The microsomal fraction was isolated from homogenate of 125I-labeled leukemia L 1210 ascites cells by filtration of postmitochondrial supernatant through a Sepharose 4 B column. It was found that the particles are labeled with iodine and show 5'-nucleotidase activity suggesting the presence of cell membranes in the fraction. The soluble proteins fraction were retarded on the column showed lactate dehydrogenase activity, and low activity of soluble beta-D-glucuronidase.
D002460:Cell Line; D002850:Chromatography, Gel; D005966:Glucuronidase / Q000378:metabolism; D007457:Iodine Radioisotopes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007945:Leukemia, Lymphoid; D008861:Microsomes / Q000201:enzymology; D009708:Nucleotidases / Q000378:metabolism; D013347:Subcellular Fractions
D007457:Iodine Radioisotopes; D007770:L-Lactate Dehydrogenase; D009708:Nucleotidases; D005966:Glucuronidase