Enhancement of liver microsome epoxide hydratase activity in rodents by treatment with 2(3)-tert-butyl-4-hydroxyanisole.
Administration of the antioxidant 2(3)-tert-butyl-4-hydroxyanisole (BHA) in the diet caused a marked increase in the specific activity of epoxide hydratase (EC 4.2.1.63) in hepatic microsomes of CD-1 mice. The increases in epoxide hydratase activities produced by BHA were far greater (11-fold) than were those produced by the administration of well-known enzyme inducers such as 3-methylcholanthrene, phenobarbital, and Aroclor 1254 (2- to 3-fold). The near-maximal increase in epoxide hydratase activity was observed after feeding of the BHA diet for 3 days. When BHA was administered by gastric intubation, the level of increase was only 75% of that attained by feeding BHA in the diet. The increase in epoxide hydratase activity produced by BHA treatment of Sprague-Dawley rats was not as pronounced (less than 3-fold) as that observed in CD-1 mice.
D000818:Animals; D000840:Anisoles / Q000494:pharmacology*; D001580:Benzopyrenes / Q000378:metabolism; D001711:Biotransformation / Q000187:drug effects; D002083:Butylated Hydroxyanisole / Q000008:administration & dosage / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004851:Epoxide Hydrolases / Q000378:metabolism*; D005260:Female; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism
D000840:Anisoles; D001580:Benzopyrenes; D002083:Butylated Hydroxyanisole; D004851:Epoxide Hydrolases