Replication of mouse mammary tumor cells in monolayer cultures stimulated with embryo extract.
A particulate fraction derived from homogenized chicken embryos was added to primary monolayer cultures of mouse mammary tumor cells. All culture media contained fetal calf serum (10%). After 4 days, the extract had stimulated a twofold increase (relative to controls lacking the extract) in (1) total DNA per culture; (2) the rate of incorporation of labeled DNA precursors; (3) epithelial cell number, and (4) total protein. This integrated growth activity is discussed in terms of the nature and exogenous regulation of neoplastic growth.
D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D002642:Chick Embryo / Q000502:physiology*; D003470:Culture Media; D046508:Culture Techniques; D004247:DNA / Q000378:metabolism; D005260:Female; D006133:Growth Substances; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D013997:Time Factors
D003470:Culture Media; D006133:Growth Substances; D004247:DNA