Synthesis of chromogranins and dopamine beta-hydroxylase by perfused bovine adrenal glands.
The incorporation of [3H]leucine into chromogranins and into soluble and membrane-bound dopamine beta-hydroxylase (DBH) was studied in isolated perfused adrenal glands. [3H]chromogranins and [3H]DBH were quantitatively determined by immunoprecipitation. The amounts of soluble [3H]DBH formed were about equal to the amounts of membrane-bound [3H]DBH whereas the amounts of [3H]chromogranin were 5- to 20-fold greater than that of soluble [3H]DBH. On continuous sucrose density gradients, [3H]chromogranin was unimodally distributed after 2- to 20-h chase periods and accumulated in a vesicle having a lower buoyant density than mature chromaffin vesicles. At 2 h both membrane and soluble [3H]DBH were both bimodally distributed whereas after a 20-h chase period the distribution of soluble and membrane [3H]DBH was essentially unimodal and paralleled the distribution of [3H]chromogranin. These studies indicate that [3H]chromogranin, soluble [3H]DBH, and membrane [3H]DBH are synthesized concomitantly, but that each is transported into chromaffin vesicles at different rates.
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000378:metabolism*; D002838:Chromaffin System / Q000378:metabolism*; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D005260:Female; D008565:Membrane Proteins / Q000096:biosynthesis; D008970:Molecular Weight; D011092:Polyethylene Glycols; D014176:Protein Biosynthesis; D012995:Solubility; D013347:Subcellular Fractions / Q000378:metabolism
D008565:Membrane Proteins; D011092:Polyethylene Glycols; D004299:Dopamine beta-Hydroxylase