Correlation of biochemical and morphologic manifestations of acute pulmonary fibrosis in rats administered paraquat.
Rats were administered 24 mg/kg of paraquat intraperitoneally. One, two, three, six and seven days after this injection, the rats were evaluated by several toxicologic, biochemical, and morphologic criteria. These included determinations of weight loss, visible lung hemorrhage, pulmonary edema, in vitro protein and collagen biosynthesis rates of lung tissue, and staining properties of lung sections. Morphologic evidence of mild edema was seen at day 1, while more severe edema was observed at days 2 through 7. Weight loss was apparent from day 1 throughout the experiment. In vitro protein biosynthesis rates by tissue were elevated above control values from day 3 onwards, while collagen biosynthesis rates were elevated from day 2 onwards. Increased staining with the Gomori reagent was observed from day 1, while increased staining with van Gieson's or Masson's trichrome appeared by day 2 or 3.
D000818:Animals; D001835:Body Weight; D003094:Collagen / Q000096:biosynthesis*; D006470:Hemorrhage / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D006651:Histocytochemistry; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D010269:Paraquat / Q000633:toxicity; D014176:Protein Biosynthesis; D011654:Pulmonary Edema / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D051381:Rats
D003094:Collagen; D010269:Paraquat