title: Application of macroscopic autoradiography to large animals in veterinary pharmacokinetics: the distribution of sodium selenite labelled with 75Se in the pig.
abstract: Macroscopic autoradiography has been applied to large animals, such as pigs. It consists in carrying out slides of different organs in well definite conditions (slide thickness, exposure rate...). Then, it is possible to compare all results with optical densitometry techniques. We have applied it to the metabolic study of sodium selenite labelled with 75Se in pigs. Autoradiography and quantification of organs as a function of time showed that for activities below 10(5) counts per minute and per gram, we get similar results. So, it is possible to use Ullberg's technique with large animals. This is very interesting in veterinary drug metabolism studies where we must determine acceptable residual levels for human consumers.
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000662:veterinary*; D003720:Densitometry; D007700:Kinetics; D004364:Pharmaceutical Preparations / Q000378:metabolism; D010600:Pharmacology / Q000379:methods*; D011868:Radioisotopes; D012643:Selenium / Q000378:metabolism; D013552:Swine / Q000378:metabolism*; D014730:Veterinary Medicine
chemical_list: D004364:Pharmaceutical Preparations; D011868:Radioisotopes; D012643:Selenium