The effects of time and temperature of storage on the infectivity of third-stage larvae of Heligmosomoides polygyrus (= Nematospiroides dubius). 1. Effects on the development to the adult stage in mice.
The relationship between storage time and temperature on the infectivity of the third-stage larvae of Heligmosomoides polygyrus (= Nematospiroides dubius) was examined. Results showed that third-stage larvae lose their infectivity when they aged. This loss was steeper at 22 degrees C than at 4 degrees C. Results obtained at 15 degrees C seemed to be intermediate. Data also demonstrated that a maturation period was required for maximum infectivity. A longer maturation period was observed at + 4 degrees C than at 22 degrees C and the maximum was maintained for longer at 4 degrees C.
D000818:Animals; D003080:Cold Temperature; D007814:Larva / Q000254:growth & development; D051379:Mice; D013696:Temperature; D013997:Time Factors; D014251:Trichostrongyloidea / Q000254:growth & development / Q000472:pathogenicity*; D014252:Trichostrongyloidiasis / Q000209:etiology* / Q000469:parasitology