[Separation of bone marrow cells of normal and anemic rabbits using isopycnic dextran density gradient].
Bone-marrow cells of normal and anemic (phenylhydrazine) rabbits were separated in the isopyknotic continuous 50-ml dextran density gradient. The separated cells were divided into 3 groups according the benzidine-peroxide-Giemsa staining after Brosook: nucleated, nonerythroid (A), nucleated, erythroid (B), and nonnucleated, erythroid (C). The highest proportion of erythroid, nucleated cells was found in fractions of medium density: in normal animals 20%, in anemic ones 45%. The group B/A ratio in the unseparated suspension is 0.7 in normal animals and 1.7 in anemic ones; and in the most favourable density fraction 0.3 and 1.9 respectively. Thus an unimportant accumulation of erythroid bone-marrow cells of rabbit is observed. In constrast, an accumulation of nucleated leukocytes of 80--90% can be reached in the low-density fractions.
D000740:Anemia / Q000139:chemically induced / Q000473:pathology*; D000818:Animals; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D010659:Phenylhydrazines; D011817:Rabbits
D010659:Phenylhydrazines
Trennung von Knochenmarkzellen normaler und anämischer Kaninchen im isopyknischen Dextran-Dichtegradienten.