[Nature of the humoral factor from bone marrow stimulating antibody production].
An active factor doubling the antibody synthesis in the culture of immune lymph node cells was isolated from the supernatant of the cultures of bone marrow cells from intact donors. Gel chromatography was used for this purpose. The stimulant of antibody producers (SAP) was eluated at the site of cytochrome C exit; the factor is heat-stable and has the mol wt of 1300 daltons. SAP production needs RNA and protein synthesis, but does not require DNA synthesis. The radioactive label showed that SAP includes ribonucleotides and amino acids. It is suggested that SAP plays an important role in the regulation of antibody synthesis.
D000818:Animals; D000917:Antibody Formation; D001853:Bone Marrow; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D006824:Hybridization, Genetic; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008937:Mitomycins / Q000494:pharmacology; D013268:Stimulation, Chemical; D014020:Tissue Extracts / Q000494:pharmacology*
D008937:Mitomycins; D014020:Tissue Extracts; D003609:Dactinomycin; D003513:Cycloheximide
Izuchenie prirody gumoral'nogo faktora kostnogo mozga, stimuliruiushchego produktsiiu antitel.