Purification and partial characterization of protein 35/7.7 a cytosol protein that is abundant in rapidly growing hepatomas.
Protein 35/7.7 is an abundant cytosol protein of Morris hepatoma 3924A and Novikoff hepatoma which was not found in normal liver. Protein 35/7.7 was isolated from the cytosol of Novikoff hepatoma ascites cells by ammonium sulfate precipitation and DEAE-cellulose chromatography. It migrated as a single major spot on two-dimensional isoelectric focusing-SDS polyacrylamide gels. The N-terminal hexapeptide is Val-Asx-Pro-Thr-Val-Phe and its carboxyl-terminal amino acid is phenylalanine.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001202:Ascitic Fluid / Q000032:analysis; D003600:Cytosol / Q000032:analysis; D008099:Liver / Q000032:analysis; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis; D051381:Rats
D000596:Amino Acids; D009363:Neoplasm Proteins; D010455:Peptides