Fusion and hybridization of marsupial and eutherian cells. Activity of heterokaryons.
Marsupial and eutherian nuclei in heterokaryons were shown to synthesize DNA and RNA, apparently at control levels, and heterokaryons were found to contain marsupial hypoxanthine-guanine phosphoribosyltransferase. The two types of nucleus in heterokaryons were also able to undergo synchronous chromosome condensation. our results provide no evidence for suppression of nucleic acid synthesis, gene expression or mitosis in marsupial x eutherian heterokaryons.
D000818:Animals; D002459:Cell Fusion; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism*; D002645:Chickens; D004247:DNA / Q000096:biosynthesis*; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000378:metabolism; D008394:Marsupialia / Q000235:genetics*; D051379:Mice; D008938:Mitosis; D012313:RNA / Q000096:biosynthesis*
D012313:RNA; D004247:DNA; D007041:Hypoxanthine Phosphoribosyltransferase