Endothelial changes in rabbit corneas. Observation by specular microscopy during storage in McCarey-Kaufman (M-K) medium.
Corneal banks are beginning to screen donor tissue with a specular microscope to detect abnormal endothelium. To determine the appearance of normal corneal endothelium under storage conditions, the endothelium of healthy rabbit corneas was examined with a modified specular microscope while being stored in McCarey-Kaufman (M-K) medium at 4 degrees C. Black areas within endothelial cells and decreased clarity of the endothelial cell borders were observed during storage and were found to be reversible on warming of the M-K medium.
D000818:Animals; D003315:Cornea / Q000166:cytology* / Q000648:ultrastructure; D003470:Culture Media; D004727:Endothelium / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D014021:Tissue Preservation
D003470:Culture Media