Analysis of A, B, E, and F prostaglandins as pentafluorobenzyl esters by electron-capture gas-liquid chromatography.
A new and sensitive method is described for the simultaneous analysis of a mixture containing PGE1, PGE2, PGF1alpha, and PGF2alpha by electron-capture gas-liquid chromatography. During derivatization of the mixture, PGE1 and PGE2 were converted to PGB1 and PGB2, respectively, yielding a mixture of PGB1, PGB2, PGF1alpha, and PGF2alpha trimethylsilyl ether pentafluorobenzyl esters. Gas chromatographic resolution of all four derivatives is sufficient for quantitation of each prostaglandin. The A prostaglandins were analyzed by similar conversion to the respective B prostaglandin derivatives. Minimum detection limits for the B and F prostaglandin derivatives were 10 pg and 1 pg, respectively. Samples of rabbit kidney medulla were incubated and analyzed for A, B, E, and F prostaglandins. The results indicate that the method is capable of high recovery and reproducibility.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D002853:Chromatography, Liquid; D004951:Esterification; D004952:Esters / Q000032:analysis; D007668:Kidney / Q000032:analysis; D011453:Prostaglandins / Q000032:analysis*; D011454:Prostaglandins A / Q000032:analysis; D011456:Prostaglandins B / Q000032:analysis; D011458:Prostaglandins E / Q000032:analysis; D011460:Prostaglandins F / Q000032:analysis; D011817:Rabbits
D004952:Esters; D011453:Prostaglandins; D011454:Prostaglandins A; D011456:Prostaglandins B; D011458:Prostaglandins E; D011460:Prostaglandins F