Synthesis and metabolism of prostaglandins E2, F2alpha and D2 by the rat gastrointestinal tract. Stimulation by a hypertonic environment in vitro.
Whole cell preparations of rat stomach corpus, jejunum, and colon were incubated and the released prostaglandin E2 (PGE2), PGF2alpha, PGD2, 15 keto-13,14 dihydro PGE2, and 15 keto-13, 14 dihydro PGF2alpha were measured by combined gas chromatography-mass spectrometry. All regions made PGD2 and possessed a high capacity for production 15 keto-13,14 dihydro derivatives of both PGE2 and PGF2alpha. Hypertonic sucrose solutions resulted in concentration-dependent increases in prostaglandin release, particularly of PGE2 and its metabolite. It is suggested that PG's may play a role in the local effects of luminal hyperosomolarity on digestive tract functions.
D000818:Animals; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism*; D006982:Hypertonic Solutions / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D011453:Prostaglandins / Q000378:metabolism*; D011457:Prostaglandins D / Q000096:biosynthesis / Q000378:metabolism*; D011458:Prostaglandins E / Q000096:biosynthesis / Q000378:metabolism*; D011460:Prostaglandins F / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors
D006982:Hypertonic Solutions; D011453:Prostaglandins; D011457:Prostaglandins D; D011458:Prostaglandins E; D011460:Prostaglandins F; D013395:Sucrose