Estrogen receptor assay of carcinomas of the breast by a simplified dextran--charcoal method.
Carcinomas of the breast from 352 women were assayed for binding of tritiated estradiol by tumor cytosol with dextran--charcoal adsorption, saturation analysis, and two-point Scatchard plots; the level of saturable binding defined a cytosol as positive or negative for estrogen receptor. Valid assays were obtained on specimens as small as 120 mg. Assays of replicate samples of a cytosol were more reproducible than assays of replicate samples of the tumor itself. Occasional disparity of results between a primary mammary carcinoma and its axillary metastases could be related to differences in tumor cellularity. Saturable binding consistent with the presence of estrogen receptor was found in 59% of 305 primary carcinomas and in 57% of 47 metastatic or recurrent carcinomas. There was a significant negative correlation between the patient's age and saturable estrogen binding in the tumor. Serum estradiol levels of less than 250 pg/ml appeared to have a negligible effect on estrogen receptor content. A small subgroup of high-binding carcinomas had high dissociation constants, but the significance of this observation is not clear.
D000367:Age Factors; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism*; D002606:Charcoal; D003911:Dextrans; D004958:Estradiol / Q000097:blood; D005260:Female; D005615:Freezing; D006801:Humans; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011960:Receptors, Estrogen / Q000032:analysis*
D003911:Dextrans; D011960:Receptors, Estrogen; D002606:Charcoal; D004958:Estradiol