Solid phase activation of alternative pathway of complement by beta-1,3-glucans and its possible role for tumour regressing activity.
The efficiency of some anti-tumour polysaccharides, such as lentinan, pachyman, pachymaran, carboxymethylpachymaran and hydroxyethylpachyman to trigger the alternative pathway of complement activation (APC) was investigated in detail, in order to clarify whether solid phase activation of APC by these polysaccharides will occur or not. From the eight polysaccharides tested, all were found to be potent activators of the alternative pathway except for carboxymethylpachymaran, regardless of their potency of inhibition of sarcoma 180 (S 180) transplanted in mice. This activation was observed both for the insoluble as well as the soluble part of the same polysaccharide. The turnover of C3, C5 and factor B showed no difference among these seven active polysaccharides. The polysaccharide particles (PX), isolated after treatment with C4d GPS showed prominent C3 consuming activity, which disappeared during prolonged incubation at 37 degrees. The activity of the decayed enzyme could be regenerated by treatment with the purified factor B. The stability of the particulate enzyme PX was comparable to the zymosan-complex (ZX) previously described.
D000818:Animals; D002634:Chemotaxis, Leukocyte; D003167:Complement Activation; D003176:Complement C3; D003182:Complement C5; D011415:Complement Factor B; D011416:Complement Factor D; D003170:Complement Pathway, Alternative; D005936:Glucans / Q000276:immunology* / Q000627:therapeutic use; D006168:Guinea Pigs; D007700:Kinetics; D007912:Lentinan / Q000276:immunology; D051379:Mice; D012510:Sarcoma 180 / Q000276:immunology* / Q000628:therapy; D012995:Solubility; D013329:Structure-Activity Relationship
D003176:Complement C3; D003182:Complement C5; D005936:Glucans; D007912:Lentinan; D011416:Complement Factor D; C493491:complement factor D, mouse; D011415:Complement Factor B