[Double labeling method for electron microscopic autoradiography].
A method for investigation of the synthesis of two different substances of the RNA and protein in the same cell was described. Two specific tritium-labeled precursors--uridine and proline were administered to the animals. The difference in the RNA and protein labeling was based on the fact that biosynthesis of these macromolecules was localized in different cell parts. Comparison of the results obtained and those of the two control experiments in which one of the precursors was administered has shown the fitness of the present method for simultaneous investigation of the RNA and protein synthesis in the wound fibroblasts.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D006097:Granulation Tissue / Q000378:metabolism*; D051379:Mice; D008854:Microscopy, Electron; D009132:Muscles / Q000293:injuries; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012867:Skin / Q000293:injuries; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D014316:Tritium; D012313:RNA; D011392:Proline; D014529:Uridine
Metod dvoĭnoĭ metki v élektronno-mikroskopicheskoĭ avtoradiografii.