[A rapid method for the isolation and quantitative analysis of 3,4-benzpyrene [benzo(a)pyrene] in food (author's transl)].
A method for the isolation and the quantitative determination of benzo(a)pyrene in food is described. The material is suspended in petroleum-ether. Benzo(a)pyrene is extracted from the organic phase as a water-soluble caffeine-complex. After re-extracting and purifying by column chromatography on silica gel the quantitative evaluation of benzo(a)pyrene is carried out by thinlayer chromatography and fluorimetric scanning of the cellulose acetate plates. To test the method benzo(a)pyrene was added to several products in concentrations of 100 to 1000 ng. A recovery of about 80% was achieved. Each analysis takes a total of 5 h.
D001580:Benzopyrenes / Q000032:analysis*; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D005470:Fluorometry; D005504:Food Analysis / Q000379:methods*
D001580:Benzopyrenes
Schnellmethode zur Isolierung und Bestimmung von 3,4-Benzypyren in Lebensmitteln.