Atomic absorption spectrometric determination of arsenic and selenium in offal and fish by hydride generation.
A method for determining As and Se in beef offal and fish was developed. The sample was digested by heating with a mixture of nitric, perchloric, and sulfuric acids. No pre-reduction of As and Se was necessary. Using a simplified generator, the metal hydrides were evolved by reduction with sodium borohydride pellets from sulfuric and hydrochloric acid media. The hydrides were swept by a flow of nitrogen into a nitrogen-hydrogen-entrained air flame. Absolute detection limit of the method was about 6 ng for As and 4 ng for Se, and absolute sensitivity for both metals was estimated to be 5 ng. Effects of the presence of several cations and anions in the matrix were investigated and some were found to have a suppressive effect on the atomic absorption signal. The analytical results obtained for samples of NBS No. 1571 Orchard Leaves and NBS No. 1577 Bovine Liver agreed well with certified values.
D000818:Animals; D001151:Arsenic / Q000032:analysis*; D001894:Borohydrides; D002417:Cattle; D005399:Fishes; D008099:Liver / Q000032:analysis; D012643:Selenium / Q000032:analysis*; D013054:Spectrophotometry, Atomic; D014866:Waste Products / Q000032:analysis
D001894:Borohydrides; D014866:Waste Products; D012643:Selenium; D001151:Arsenic