Complete amino acid sequence of the precursor region of rat prolactin.
Rat preprolactin was translated in a wheat germ in vitro synthetic system in the presence of 3H-labeled amino acids. The amino-terminal sequence of the radiolabeled precursor was quantitatively identified by high pressure liquid chromatography. The 29 residue precursor sequence is: Met-Asn-Ser-Gln-Val-Ser-Ala-Arg-Lys-Ala-Gly-Thr-Leu-Leu-Leu-Leu-Met-Met-Ser-Asn-Leu-Phe-Cys-Gln-Asn-Val-Gln-Thr-. A discussion of the structure-function relationships of the precursor region and the microsequencing methods used to determine the sequence is presented. The amino-terminal 30 residue sequence of the secreted form of rat prolactin was determined and found to differ at positions 7 and 8 (-Gly-Asp-) from a previously reported rat prolactin sequence (Parlow, A.F., & Shome, B. (1.76) Fed. Proc., Fed. Am. Soc. Exp. Biol. 35, 219).
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002474:Cell-Free System; D011388:Prolactin / Q000032:analysis / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000032:analysis / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
D000596:Amino Acids; D011498:Protein Precursors; D012333:RNA, Messenger; D011388:Prolactin