The effect of dietary fat or cholesterol and cholic acid on the rate of synthesis of rat liver glucose-6-P dehydrogenase.
The effect of dietary fat or cholesterol and cholic acid on the rate of synthesis of rat liver glucose-6-P dehydrogenase was measured by isolating hepatocytes from the livers of rats fed diets containing various amounts and types of lipid. Hepatocyte proteins were labeled with [3H]leucine and glucose-6-P dehydrogenase was immunoprecipitated with a specific antiserum. The immunoprecipitates were electrophoresed on sodium dodecyl sulfate-polyacrylamide gels and the radioactivity in the glucose-6-P dehydrogenase subunit was used to calculate the relative rate of synthesis of the enzyme. It is concluded that the activity of glucose-6-P dehydrogenase is primarily regulated by alterations in the rate at which the enzyme is synthesized. Dietary fat may decrease the rate of degradation of glucose-6-P dehydrogenase.
D000818:Animals; D002791:Cholesterol, Dietary; D002793:Cholic Acids / Q000494:pharmacology*; D004041:Dietary Fats / Q000008:administration & dosage*; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis* / Q000302:isolation & purification; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008297:Male; D009821:Oils / Q000494:pharmacology; D051381:Rats; D003313:Zea mays
D002791:Cholesterol, Dietary; D002793:Cholic Acids; D004041:Dietary Fats; D046911:Macromolecular Substances; D009821:Oils; D005954:Glucosephosphate Dehydrogenase