[Modification of drug oxidation in rat liver microsomal fractions by mitochondria].
In order to establish possible interaction between mitochondrial membranes and endoplasmic reticulum, the effects of succinate, fumarate and malate (10(-6)--10(-2) M) on N-demethylation of ethylmorphine in liver homogenate, liver homogenate, enriched by mitochondria and in liver sections of newborn animals were studied. In experiments with liver homogenate and liver mitochondria-enriched homogenate the substrates exerted no effect. Addition of mitochondria decreased the homogenate activity by 50%, when NADPH was used as cosubstrate. Studies on liver sections showed the increase of activity only in case of succinate. The activity of liver sections from newborn rate was 30% of that from adult animals. The enzyme activity was suppressed by the Krebs cycle substrates.
D000818:Animals; D000831:Animals, Newborn; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005037:Ethylmorphine-N-Demethylase / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D051381:Rats; D013386:Succinates / Q000378:metabolism
D005650:Fumarates; D008293:Malates; D013386:Succinates; D010089:Oxidoreductases, N-Demethylating; D005037:Ethylmorphine-N-Demethylase
Modifikatsiia mitokhondriiami okisleniia lekarstvennykh veshchestv v mikrosomnoĭ fraktsii pecheni krys.