[Distribution of 5'-nucleotidase in fractions of rat yolk-sac after differential centrifugation and isopycnic sucrose gradient centrifugation].
The 5'-nucleotidase considered as a marker enzyme of the plasma membranes shows a highly specific acitvity in both nuclear and mitochondrial-lysosomal fractions of the rat yolk-sac. In isopycnic sucrose gradient fractionation of the mitchondrial-lysosomal fraction a relative accumulation of this enzyme could be shown in the density level of the phagolysosomes (d = 1,16--1,20).
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient; D005260:Female; D009708:Nucleotidases / Q000032:analysis*; D051381:Rats; D014461:Ultracentrifugation; D015017:Yolk Sac / Q000201:enzymology* / Q000648:ultrastructure
D009708:Nucleotidases
Verteilung der 5'-Nukleotidase in Fraktionen des Rattendottersacks nach Differentialzentrifugation und isopyknischer Saccharose-Gradientenzentrifugation.