title: Subcellular localization of two long-chain acyl-coenzyme-A synthetases in Candida lipolytica.
abstract: Studies have been made on the subcellular localization of two long-chain acyl-coenzyme-A synthetases as well as glycerolphosphate acyltransferase and the acyl-CoA-oxidizing system in Candida lipolytica grown on oleic acid. Acyl-CoA synthetase I is distributed among different subcellular fractions, including microsomes and mitochondria where glycerolphosphate acyltransferase is located. On the other hand, acyl-CoA synthetase II is localized in microbodies where the acyl-CoA-oxidizing system is located. These results support our previous conclusion that acyl-CoA synthetase I is responsible for the production of acyl-CoA to be utilized for the synthesis of cellular lipids, while acyl-CoA synthetase II provides acyl-CoA that is exclusively degraded via beta-oxidation.
mesh_terms: D002175:Candida / Q000201:enzymology* / Q000648:ultrastructure; D002458:Cell Fractionation; D003066:Coenzyme A Ligases / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D006651:Histocytochemistry; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D003066:Coenzyme A Ligases