Determination of 3-methoxy-4-hydroxyphenylpyruvic acid, 3,4-dihydroxyphenylethylene glycol, and 3,4-dihydroxyphenylmandelic acid in urine by mass fragmentography, with use of deuterium-labeled internal standards.
We report the determination of 3-methoxy-4-hydroxyphenylpyruvic acid, 3,4-dihydroxyphenylmandelic acid, and 3,4-dihydroxyphenylethylene glycol in urine, by use of gas chromatography/mass spectrometry in combination with a simple purification method and deuterium-labeled internal standards. Normal excretion values in terms of creatinine, expressed as a function of age, are given, together with results obtained for patients with neuroblastoma, pheochromocytoma, or parkinsonism treated with L-DOPA + peripheral decarboxylase inhibitor, and for a patient receiving dopamine. We were unable to identify 3, 4-dihydroxyphenyllactic acid in urine. The results obtained and their relation to other catecholamine metabolites and catecholamine-precursor metabolites in urine are discussed.
D000293:Adolescent; D000310:Adrenal Gland Neoplasms / Q000652:urine; D000328:Adult; D000368:Aged; D002396:Catechols / Q000652:urine; D002648:Child; D002675:Child, Preschool; D003903:Deuterium; D004298:Dopamine / Q000627:therapeutic use; D005026:Ethylene Glycols / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007980:Levodopa / Q000627:therapeutic use; D008333:Mandelic Acids / Q000652:urine*; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives; D008875:Middle Aged; D009447:Neuroblastoma / Q000652:urine; D010300:Parkinson Disease / Q000188:drug therapy / Q000652:urine; D010667:Phenylpyruvic Acids / Q000652:urine*; D010673:Pheochromocytoma / Q000652:urine; D012015:Reference Standards; D012016:Reference Values; D012770:Shock, Cardiogenic / Q000188:drug therapy
D002396:Catechols; D005026:Ethylene Glycols; D008333:Mandelic Acids; D010667:Phenylpyruvic Acids; C016827:vanilpyruvic acid; D007980:Levodopa; D008734:Methoxyhydroxyphenylglycol; D003903:Deuterium; C010117:3,4-dihydroxyphenylglycol; D004298:Dopamine