Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels.
A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.
D000936:Antigen-Antibody Complex; D002696:Chlamydomonas / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D008722:Methods; D014404:Tubulin / Q000032:analysis*
D000936:Antigen-Antibody Complex; D006023:Glycoproteins; D008565:Membrane Proteins; D014404:Tubulin