Separation of retinoid receptors from cultured retinoblastoma cells.
Receptor proteins for [3H]retinol and [3H]retinoic acid in cultured human retinoblastoma cells have been separated rapidly and reproducibility by two different methods. By isoelectric focusing, the isoelectric point of the retinol receptor is at pH 4.0; the retinoic acid receptor has a higher isoelectric point of 4.3. Polyacrylamide slab gel electrophoresis revealed a slower migration rate for the [3H]retinoic acid receptor compared to the [3H]retinol receptor. The separate nature of the two proteins has thus been established in this unique human cell line.
D002352:Carrier Proteins / Q000302:isolation & purification; D002460:Cell Line; D009374:Neoplasms, Experimental / Q000032:analysis; D011955:Receptors, Drug / Q000302:isolation & purification*; D012175:Retinoblastoma / Q000032:analysis*; D014212:Tretinoin / Q000378:metabolism; D014801:Vitamin A / Q000378:metabolism*
D002352:Carrier Proteins; D011955:Receptors, Drug; D014801:Vitamin A; D014212:Tretinoin