Determination of heme and non-heme iron content of mouse erythropoietic organs.
A procedure is described for determining the content of heme and non-heme iron in organs and tissues of the mouse. Heme iron after homogenization and hemolysis of the samples is extracted as hemin by means of a mixture of ethyl acetate and glacial acetic acid, the extraction being followed by washing with hydrochloric acid. Total iron content is determined with commercial analytical sets exploiting color reaction with bathophenanthroline after mineralization of the samples. The presence of bone or other tissues does not distort the results. The procedure is relatively simple and suitable for serial analyses. The results obtained for the mouse by this method are in good agreement with recognized principles of ferrokinetics in mammals.
D000818:Animals; D001853:Bone Marrow / Q000032:analysis*; D006418:Heme / Q000032:analysis*; D006427:Hemin / Q000032:analysis / Q000302:isolation & purification; D007202:Indicators and Reagents; D007501:Iron / Q000032:analysis* / Q000097:blood; D008099:Liver / Q000032:analysis*; D008722:Methods; D051379:Mice; D008810:Mice, Inbred C57BL; D013053:Spectrophotometry; D013154:Spleen / Q000032:analysis*
D007202:Indicators and Reagents; D006418:Heme; D006427:Hemin; D007501:Iron