Specific termination of RNA polymerase synthesis as a method of RNA and DNA sequencing.
Termination of RNA synthesis with 3'-O-Methylnucleoside 5'-triphosphates have been studied using E. coli RNA polymerase holoenzyme and poly [d(A-T)] as well as unfractionated T7 D delta III DNA as templates. It was shown that the termination can be used for DNA sequencing. A sequence of a part of RNA synthesized from AI promoter of the DNA have been determined. Syntheses of four 3'-O-Methylnucleoside 5'-triphosphates are described.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives; D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D003570:Cytidine Triphosphate / Q000031:analogs & derivatives; D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives; D008722:Methods; D008745:Methylation; D011067:Poly dA-dT / Q000378:metabolism; D012265:Ribonucleotides / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000031:analogs & derivatives
D004279:DNA, Viral; D012265:Ribonucleotides; D011067:Poly dA-dT; D003570:Cytidine Triphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases; D014544:Uridine Triphosphate