[Sensitivity study of dog kidney membrane-bound ribosomes to the action of amphotericin B in vitro].
The effect of amphotericin B on the number of the ribosomes bound with the endoplasmic membranes in the dog kidney cells was studied in vitro. The binding of the ribosomes was evident from the RNA/protein ratio. It was found that the RNA/protein ratio in the rough membranes treated with amphotericin B in a concentration of Igamma/ml remained at the normal level. An increase in the antibiotic concentration up to 100 gamma/ml did not either change the number of the ribosomes bound to the membranes. On the basis of the results of the study it was possible to conclude that the toxic effect of amphotericin B on the kidneys was not associated with damaging of the ribosomal-membrane complexes in the kidney cells.
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D004285:Dogs; D004721:Endoplasmic Reticulum / Q000187:drug effects; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects*; D011485:Protein Binding / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects*
D000666:Amphotericin B
Issledovanie chuvstvitel'nosti membranosviazannykh ribosom pochek sobak k deĭstviiu amfoteritsina B in vitro.