Study of nuclear mannosyl-transferase: lipids intermediates.
Mannosyl-transferase of rat liver nuclei catalyzed the transfer of mannose, from GDP-mannose, to endogeneous lipids and proteins. Two solubility-different glycolipids were characterized: a mannosyl-phosphoryl-dolichol and an oligosaccharide-lipid. The labeling of the two lipids was consistent with a role in mannose transfer to nuclear glycoproteins, but not according to the Lennarz' scheme.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D004287:Dolichol Monophosphate Mannose / Q000096:biosynthesis / Q000302:isolation & purification; D006017:Glycolipids / Q000096:biosynthesis* / Q000302:isolation & purification; D006023:Glycoproteins / Q000096:biosynthesis; D006602:Hexosyltransferases / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008358:Mannose / Q000378:metabolism; D008364:Mannosyltransferases / Q000378:metabolism*; D009711:Nucleotides / Q000494:pharmacology; D051381:Rats
D006017:Glycolipids; D006023:Glycoproteins; D009711:Nucleotides; D004287:Dolichol Monophosphate Mannose; D006602:Hexosyltransferases; D008364:Mannosyltransferases; D008358:Mannose