Fluoride, fluoride resistance and glycolysis in cultured cells.
Long-term (several years) exposure to fluoride induced decreased production of lactate in cultured, fluoride resistant LS cells. In intact, sensitive cells, 6 mM NaF had no effect on glycolysis, whereas in homogenates, from both resistant and sensitive cells, lactate production was blocked by 6 mM NaF, indicating the cell membrane to be a barrier to fluoride. A lower intracellular rather than extracellular fluoride concentration was found in the sensitive cell with a ratio of 0.4, whereas fluoride resistant cells excluded fluoride from their intracellular milieu.
D000818:Animals; D002463:Cell Membrane Permeability; D002477:Cells / Q000378:metabolism*; D002478:Cells, Cultured; D004351:Drug Resistance; D005459:Fluorides / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects*; D007773:Lactates / Q000096:biosynthesis; D051379:Mice; D013997:Time Factors
D007773:Lactates; D005459:Fluorides