Isolation of immunoreactive components from experimental and human tumour tissues and serums by high-performance gel chromatography.
A method for the rapid separation of proteinous fractions by high-performance gel chromatography was described. Homogenates from tumorous and healthy tissues and blood were eluted by saline on a column packed with rigid hydrophilic macroporous particles of O-glucose--Spheron 300. Fractions were collected and subjected to further analyses. Their antigenic activity was determined by the leucocyte adherence inhibition test method. For the specific immunoactive fractions a dependence of leucocyte adherence inhibition test values on the clinical state of sample donators has been found.
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D005260:Female; D006801:Humans; D007120:Immunochemistry; D007957:Leukocyte Adherence Inhibition Test; D008297:Male; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis* / Q000097:blood; D009374:Neoplasms, Experimental / Q000032:analysis / Q000097:blood
D009363:Neoplasm Proteins