The effect of D-galactosamine on plasma protein synthesis by the perfused rat liver from turpentine-stimulated donors.
D-galactosamine (100 mg) was added to the reconstituted blood during 4h perfusion of livers isolated either from control rats or those injected with turpentine 20 h or 5 h earlier. This dose of galactosamine administered 30 min before [3H]lysine significantly inhibited the incorporation of the label into liver proteins, and even more into plasma proteins, but albumin and acute-phase reactants (fibrinogen, seromucoid fraction, Concanavalin A-adsorbed glycoproteins) were all similarly affected. When galactosamine was administered in vivo simultaneously with turpentine, and the liver was isolated 5 h later, trauma-induced fibrinogen synthesis was selectively inhibited. This can be explained either by a differential control of synthesis of various acute-phase reactants, or by augmentation of catabolism of fibrinogen in galactosamine-treated rats. Crossed immunoelectrophoresis of the full perfusate or Concanavalin A-adsorbed glycoproteins did not reveal any significant effect of galactosamine on the protein pattern obtained from control or turpentine-stimulated liver donors.
D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis*; D005340:Fibrinogen / Q000096:biosynthesis; D005688:Galactosamine / Q000494:pharmacology*; D006023:Glycoproteins / Q000096:biosynthesis*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D012709:Serum Albumin / Q000096:biosynthesis; D014425:Turpentine
D001798:Blood Proteins; D006023:Glycoproteins; D012709:Serum Albumin; D005688:Galactosamine; D005340:Fibrinogen; D014425:Turpentine