Intercalary regeneration around the circumference of the cockroach leg.
Epidermal cells from different circumferential positions around the femur of Blabera craniifer can interact to form an intercalary regenerate. Removal of a longitudinal strip of integument (cuticle plus epidermis) from any position around the circumference leads to the cut edges healing, localized growth and intercalary regeneration of the missing section of the circumference, so that the resulting femur is approximately normal in size and pattern of cuticular structures. Grafting a longitudinal strip of femur integument into a different circumferential position on the host femur confronts epidermal cells from different positions along both the inner and outer longitudinal graft/host junctions. In numerous different situations this results in local growth and intercalary regeneration of that section of the circumference normally separating graft and host positions, by the shorter route around the circumference. Confrontation of opposite positions results in the intercalation of either of the intervening half circumferences. In one opposite confrontation, between mid-anterior and mid-posterior, there was also a third result where graft and host healed together, provoking no intercalary regeneration. Grafts made with reversed proximal/distal polarity show that a confrontation between different circumferential positions gives the same result, regardless of the proximal/distal levels involved, hence circumferential position is an independent aspect of position on the femur. These results strongly suggest that epidermal position is not specified with respect to two transverse axes running through the epidermis and internal tissue of the leg, but that there is a continuous circular sequence of positional values running around the circumference, in the epidermis. This is analogous to but independent of the sequence previously shown by Bohn (1967) and Bullière (1971) to run proximal/distal along a leg segment. Hence epidermal position on the femur is specified in two dimensions and can be represented in terms of the French, Bryant & Bryant (1976) polar co-ordinate model. Interactions along the edges of the strip-grafts conform to the Shortest Intercalation Rule (French et al. 1976). At the proximal and distal ends of strip-grafts intercalation restores normal sequences of positional values where possible. However, where the graft, together with the intercalary regenerates formed at the longitudinal graft/host junctions and the adjacent host tissue formed a complete sequence of circular values, then a supernumerary distal regenerate was formed, in agreement with the Complete Circle Rule of French et al. (1976). The problem of generating a continuous circular sequence of positional values by one or more circumferential gradients, is briefly discussed.
D000818:Animals; D002686:Chitin; D003058:Cockroaches / Q000502:physiology*; D004817:Epidermis / Q000637:transplantation; D005121:Extremities / Q000502:physiology*; D007814:Larva; D012038:Regeneration; D014182:Transplantation, Autologous
D002686:Chitin