[Method of studying the functional state of pulmonary alveolar macrophages during exposure to atmospheric pollutants].
On the basis of experimental research results a method to assess the functional state of pulmonary alveolar macrophages in rabbits and rats has been proposed as a criterion of the biological effect of chemical atmospheric pollutants. The test involves a cytological assay, determination of the viable cells quantity and of the phagocytic competence, and also the biochemical study of alveolar macrophages enzymes activity (acid phosphatase, beta-glucuronidase, lysozyme, beta-galactosidase, beta-glucosidase, N-acetyl-beta-D-glucosaminidase). It has been shown that this method is informative and reliably reproducible, and that it was reasonable to use it in environmental health and other branches of experimental biology and medicine.
D000135:Acid Phosphatase / Q000378:metabolism; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D002452:Cell Count; D005696:Galactosidases / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism; D008264:Macrophages / Q000201:enzymology* / Q000276:immunology; D009113:Muramidase / Q000378:metabolism; D010587:Phagocytosis; D011650:Pulmonary Alveoli / Q000166:cytology*; D011817:Rabbits; D051381:Rats
D000393:Air Pollutants; D000135:Acid Phosphatase; D005696:Galactosidases; D005959:Glucosidases; D006596:Hexosaminidases; D009113:Muramidase; D005966:Glucuronidase
Metod izucheniia funktsional'nogo sostoianiia al'veoliarnykh makrofagov legkikh pri vozdeĭstvii atmosfernykh zagriaznenii.