Reconstitution of 5-hydroxytryptamine transport from cholate-disrupted platelet plasma membrane vesicles.
The bile acid cholate disrupts platelet plasma membrane vesicles and inactivates 5-hydroxytryptamine transport activity. The transporter appears not to be solubilized by this procedure, but remains associated with a high molecular weight aggregate. Merely removing cholate does not restore the vesicular structure or transport activity, but in the presence of added soybean phospholipids, cholate removal generates proteoliposomes which accumulate 5-hydroxytryptamine. This reconstituted transport activity is similar in its ionic requirements and inhibitor sensitivity to transport in native membrane vesicles and intact platelets.
D001692:Biological Transport; D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002793:Cholic Acids / Q000494:pharmacology*; D006801:Humans; D010743:Phospholipids / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology
D002793:Cholic Acids; D010743:Phospholipids; D012701:Serotonin; D012964:Sodium; D011188:Potassium