[Formation of pyruvic and lactic acids in muscles diluted homogenate differing from the generally accepted glycolysis scheme].
When studying formation of pyruvic and lactic acids from fructose diphosphate the effect of dilution is found in the process of muscles homogenate incubation. Pyruvic acid accumulates only in samples with magnesium chloride added. The increase of lactic acid remains constant irrespectively of availability of conditions for pyruvate formation. Phosphoglycerate and phosphoenolpyruvate form pyruvic acid only in samples with magnesium chloride added without forming lactic acid. It is shown that in the muscles diluted homogenate (25 mg per 6 ml of mixture) lactic acid is formed under conditions unsuitable for pyruvic acid formation. Lactic acid is formed omitting the stages of 2-phosphoglycerate, phosphoenolpyruvate and pyruvate i.e. in the pathway different from a classical scheme of glycolysis.
D000818:Animals; D001001:Anura; D006019:Glycolysis / Q000187:drug effects; D007773:Lactates / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D009132:Muscles / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D011817:Rabbits; D051381:Rats
D007773:Lactates; D011773:Pyruvates; D008274:Magnesium
Obrazovanie pirovinogradnoĭ i molochnoĭ kislot v razvedennom Gomogenate myshts otlichnym ot obshchepriniatoĭ skhemy glikoliza putem.