title: A technique for detection and relative quantitative analysis of glucosephosphate isomerase isozymes from nanogram tissue samples.
abstract: An improved method for detecting and measuring the enzyme glucosephosphate isomerase after starch gel electrophoresis is described. Nitrocellulose filters are used in a gel overlay procedure which increases the sensitivity of the staining reaction and provides a simple means for accurate quantitation of the isozyme pattern. This staining technique may have wider application with other gel media and also with other enzymes.
mesh_terms: D000483:Alleles; D000818:Animals; D004592:Electrophoresis, Starch Gel; D005260:Female; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis* / Q000235:genetics; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D008297:Male; D051379:Mice; D008832:Microchemistry
chemical_list: D007527:Isoenzymes; D005956:Glucose-6-Phosphate Isomerase