Correlation between in vitro and in vivo drug metabolism rate: oxidation of ethoxybenzamide in rat.
In vitro and in vivo correlations of the microsomal oxidation of drugs were examined, using ethoxybenzamide as a model drug. Ethoxybenzamide disappearance time course from rat plasma in vivo was analyzed by a two-compartment model assuming a Michaelis-Menten type elimination process. Ethoxybenazmide oxidation in vitro was measured by the appearance rate of salicylamide in rat liver microsomal suspension. Parameters obtained were Vmax = 3.46 and 3.77 mumoles/min/kg body weight and Km = 0.378 and 0.192 mM, in vitro and in vivo, respectively.
D000818:Animals; D001549:Benzamides / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism; D005019:Ethyl Ethers / Q000378:metabolism*; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000378:metabolism; D051381:Rats; D014018:Tissue Distribution
D001549:Benzamides; D001798:Blood Proteins; D005019:Ethyl Ethers