title: Lymphocyte subpopulations in man: no selective loss during isolation from defibrinated blood.
abstract: Human lymphocytes isolated from defibrinated blood are characterized especially in relation to the total yield of lymphocytes and their subpopulations identified by membrane markers. The defibrination per se gives a significant loss of monocytes and granulocytes but no loss of lymphocytes, indicating that no selective loss of lymphocytes occurs. In a comparison of heparin-stabilized and defibrinated blood no difference in yield is found during every single step of the isolation procedure. Quantitation of E-RFC and SmIg-positive lymphocytes gives no differences in comparing the respective isolated suspensions of mononuclear cells. The observed difference in EA- and EAC-RFC can freely be ascribed to the difference in monocyte contamination. Further, no correlation is found between total lymphocyte yield and the relative number of the subpopulations identified, i.e. E-RFC, SmIg-positive, Fc-receptor- and complement-receptor-bearing lymphocytes. Hence it is concluded that defibrinated blood is optimal as blood source for the isolation of mononuclear cells when lymphocyte subpopulations are studied and enumerated.
mesh_terms: D002469:Cell Separation; D005337:Fibrin; D006493:Heparin; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell; D012397:Rosette Formation
chemical_list: D011947:Receptors, Antigen, B-Cell; D005337:Fibrin; D006493:Heparin