The interaction of mammalian interferons with immobilized cibacron blue F3GA: modulation of binding strength.
Mouse, hamster, rabbit, horse, and human interferons bind to immobilized Cibacron Blue F3GA under appropriate solvent conditions. Three forms of the immobilized ligand have been investigated: Cibacron Blue F3GA-Sepharose 4B, Blue Dextran-Sepharose 4B and Blue Sepharose CL-6B. The strength of binding of an interferon depends critically on the sorbent: Cibacron Blue F3GA-Sepharose 4B is the weakest in the series and Blue Sepharose CL-6B the strongest. The use of Blue Dextran-Sepharose 4B--a sorbent of intermediate binding properties--allows the complete separation of hamster, mouse and human fibroblast interferons in a single chromatographic step. Indeed, both the resolution, as well as the recovery, of those interferons is complete--regardless of the relative complexity of the chromatographed preparation (containing either crude or purified interferons). Thus, these ligands should prove of considerable use when a facile chromatographic evaluation, both qualitative and quantitative of mammalian interferons is required.
D000818:Animals; D000873:Anthracenes; D002846:Chromatography, Affinity; D004396:Coloring Agents; D006801:Humans; D007372:Interferons; D008024:Ligands; D013045:Species Specificity; D014227:Triazines
D000873:Anthracenes; D004396:Coloring Agents; D008024:Ligands; D014227:Triazines; C012842:Cibacron Blue F 3GA; D007372:Interferons