Affinity chromatography of the phosphatidylcholine exchange protein from bovine liver.
Affinity chromatography has been used to purify the phosphatidylcholine exchange protein from bovine liver. The affinity resin consisted of 1-acyl-2-(9-carboxy)nonyl-glycero-3-phosphocholine linked to AH-Sepharose 4 B via the carboxyl group. Application of a crude exchange protein fraction to the affinity column resulted in a complete adsorption of the phosphatidylcholine exchange protein. The exchange protein eluted with a buffer containing 0.15% sodium deoxycholate. The most active fraction was 130-fold purified and accounted for 62% of the activity.
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D008099:Liver / Q000032:analysis* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*
D002352:Carrier Proteins; D010713:Phosphatidylcholines