Purification of the protein crystal from Bacillus thuringiensis by zonal gradient centrifugation.
A method is described for the large-scale purification of the Bacillus thuringiensis protein crystal by zonal gradient centrifugation. NaBr gradients are employed in a Beckman J21-B centrifuge equipped with a JCF-Z rotor.
D001413:Bacillus thuringiensis / Q000032:analysis*; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D002501:Centrifugation, Zonal / Q000379:methods; D003461:Crystallography; D007306:Insecticides; D013171:Spores, Bacterial / Q000032:analysis
D001426:Bacterial Proteins; D007306:Insecticides