DNA damage in liver, colon, stomach, lung and kidney of BALB/c mice treated with 1,2-dimethylhydrazine.
DNA single-strand breaks induced in various organs of BALB/c mice by treatment with a single dose of 1,2-dimethylhydrazine (DMH) were studied by means of the alkaline elution method modified in order to allow the evaluation of DNA damage in vivo with no need of radioactive prelabelling. DNA damage was detected in liver, lung, kidney, stomach and colon mucosa, with the liver showing the greatest amount of damage. Its degree was dependent on the dose and route of administration. A differential effect was evident in colon mucosa from Swiss and C57BL/6 mice which are respectively susceptible and resistant to the induction of bowel tumors by DMH. The higher degree of DNA damage found in liver in comparison with colon mucosa is consistent with the previously reported higher degree of DNA methylation, but does not correlate with the specificity of this carcinogen in inducing tumors of the large intestine in mice given repeated subcutaneous injections.
D000818:Animals; D003106:Colon / Q000187:drug effects*; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000378:metabolism; D004127:Dimethylhydrazines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007668:Kidney / Q000187:drug effects*; D008099:Liver / Q000187:drug effects*; D008168:Lung / Q000187:drug effects*; D008297:Male; D008745:Methylation; D008763:Methylhydrazines / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D013045:Species Specificity; D013270:Stomach / Q000187:drug effects*; D014018:Tissue Distribution
D004277:DNA, Single-Stranded; D004127:Dimethylhydrazines; D008763:Methylhydrazines; D004247:DNA