Growth of Babesia bovis in bovine erythrocyte cultures.
Babesia bovis was cultured in a suspension of bovine erythrocytes incubated at 37 degrees C in Medium 199 with 50% bovine serum. The cells in culture were kept in suspension by slow stirring in spinner flasks and the medium was replaced at 24-hour intervals. Persistent multiplication of the parasite in a short series of subcultures suggests the feasibility of this approach for continuous culture.
D000818:Animals; D001403:Babesia / Q000254:growth & development*; D001404:Babesiosis / Q000469:parasitology*; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D004912:Erythrocytes / Q000469:parasitology*
D003470:Culture Media