Carcinogenesis in tissue culture. 29: Neoplastic transformation of a normal human diploid cell strain, WI-38, with Co-60 gamma rays.
WI-38 normal human diploid fibroblasts were exposed to Co-60 gamma rays 4 times at a total dose of 1400 rads and transformed into neoplastic cells in culture. The transformed WI-38 cells which are growing steadily without showing aging phenomena at the present time showed epithelial-like morphology, abnormal karyology, B-type isoenzyme pattern of glucose-6-phosphate dehydrogenase (G6PD), and produced sarcomas when transplanted into cheek pouches of hamsters treated with anti-hamster thymocyte serum.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D002869:Chromosome Aberrations; D003037:Cobalt Radioisotopes; D006224:Cricetinae; D046508:Culture Techniques; D004171:Diploidy; D004307:Dose-Response Relationship, Radiation; D006801:Humans
D003037:Cobalt Radioisotopes