Histone-DNA interactions within chromatin. Isolation of histones from DNA-histone adducts induced in nuclei by UV light.
We have developed a method by which to isolate histones that have been crosslinked to DNA following irradiation of calf thymus nuclei by UV light. The procedure involves separation of protein-DNA adducts from uncrosslinked protein by Sepharose 4B chromatography under dissociating conditions. Histones which are crosslinked to DNA are released by chemical hydrolysis of the DNA and identified by SDS gel electrophoresis. The results indicate that, of the histones, H1 and H3 become crosslinked to the DNA most readily under our irradiation conditions.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000528:radiation effects*; D002843:Chromatin / Q000528:radiation effects*; D004247:DNA / Q000528:radiation effects*; D006657:Histones / Q000528:radiation effects*; D008970:Molecular Weight; D011485:Protein Binding; D013950:Thymus Gland; D014466:Ultraviolet Rays
D002843:Chromatin; D006657:Histones; D004247:DNA