title: Discrepancy between one-stage and two-stage assay of factor VIII:C.
abstract: Two methods (one-stage and two-stage) are commonly used for the assay of factor VIII clotting activity (VIII:C). We present collected data from seven separate studies of VIII:C assay which show that these methods do not give the same result when comparing concentrates and plasma. On average, two-stage assays detect 20% more VIII:C activity in concentrates as compared to plasmas than do one-stage assays.
mesh_terms: D005169:Factor VIII / Q000032:analysis*; D005612:Freeze Drying; D006403:Hematologic Tests / Q000592:standards; D006801:Humans; D007391:International Cooperation; D008722:Methods
chemical_list: D005169:Factor VIII