A constant-volume ultrafiltration technique for the calculation of equilbrium binding data.
A constant-volume ultrafiltration technique is described, and details of its assessment presented. The retention characteristics of two membranes were evaluated using molecules of known molecular weight. The technique is rapid, precise, economical of material and yields equilibrium data. In these respects, it compares favourably with conventional techniques such as equilibrium dialysis.
D000418:Albumins / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D006874:Hydrostatic Pressure; D008024:Ligands; D008970:Molecular Weight; D011485:Protein Binding; D014462:Ultrafiltration / Q000379:methods*
D000418:Albumins; D008024:Ligands; D002118:Calcium