Site of iodination in rat mammary gland.
The ability of the mammary gland to take up and organically bind radioiodide was studied in non-pregnant, pregnant, and lactating rats. Autoradiography was used to determine whether duct cells or alveolar cells are responsible for iodination in the rat mammary gland. Iodination was not detected in mammary glands from non-pregnant rats, but occurred late in the twelfth day of gestation and continued throughout pregnancy and lactation. Protein-containing vacuoles in alveolar cells and casein-like proteins in milk were the major sites where iodination occurred within the gland. Milk proteins in the lumens of ductules adjacent to alveoli were also iodinated. In contrast, ducts, myoepithelial cells, fat cells, blood vessels and other histological components of the gland did not show iodinating capability. Cytochemistry was also used to identify endogenous mammary peroxidase activity in the same glands, and it was found that the presence and location of this enzyme was correlated with the ability to iodinate.
D000818:Animals; D005260:Female; D007454:Iodides / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007774:Lactation; D008321:Mammary Glands, Animal / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D008894:Milk Proteins / Q000378:metabolism; D010544:Peroxidases / Q000032:analysis; D011247:Pregnancy; D011270:Pregnancy, Animal; D051381:Rats; D014617:Vacuoles / Q000378:metabolism
D007454:Iodides; D007457:Iodine Radioisotopes; D008894:Milk Proteins; D010544:Peroxidases