[Establishment of a new breeding colony of germfree CF no. 1 mice (author's transl)].
Baby CF no. 1 mice were obtained by hysterectomy and reared by hand feeding under aseptic condition. Temperature in the cage was kept at 33 degrees C for first 14 days of age. Artificial milk, diet RM was composed of rat milk (44%) collected from the stomach of killed baby rats, evaporated cow milk (16%) and vitamin mixture (2%). The diet was homogenized after sterilization and given mice using a latex gum stomach tube at intervals of four hours for 20 to 24 days of age. Feeding size a day (Y ml) was given by the following regression curve, Y = 0.412 X - 0.299, (X : body weights in gram). According to this procedure, 40 out of 74 germfree mice (54%) were weaned with good growth. From a male and a female mice, the germfree CF no. 1 mouse colony of Takeda was established in 1967 and the lines were distributed to several laboratories of this country.
D000821:Animal Feed; D000822:Animal Husbandry; D000818:Animals; D001835:Body Weight; D005260:Female; D005856:Germ-Free Life; D008297:Male; D008722:Methods; D051379:Mice; D008815:Mice, Inbred Strains; D012098:Reproduction