Release of [3H-noradrenaline from the motor adrenergic nerves of the anococcygeus muscle by lysergic acid diethylamide, tyramine or nerve stimulation.
1 A method is described for labelling the neuronal noradrenaline (NA) stores of rat anococcygeus with [3H]-NA and detecting subsequent release of 3H from the superfused tissue by nerve stimulation or drugs. 2 Lysergic acid diethylamide (LSD) or tyramine but not barium chloride or carbachol increased the efflux of 3H although each drug produced an equivalent contractile response. This confirms that LDS has an indirect sympathomimetic action. 3 LSD was found to produce a proportionately smaller reduction of the nerve-induced efflux of 3H than of the accompanying contractile response. 4 The inhibition of nerve-induced contractile responses by LSD was shown to be independent of the neuronal uptake of noradrenaline and any post-junctional inhibition demonstrated to be non-specific.
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002415:Cats; D003042:Cocaine / Q000494:pharmacology; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008238:Lysergic Acid Diethylamide / Q000494:pharmacology*; D009046:Motor Neurons / Q000378:metabolism*; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000378:metabolism* / Q000502:physiology; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D014439:Tyramine / Q000494:pharmacology*
D008238:Lysergic Acid Diethylamide; D002217:Carbachol; D003042:Cocaine; D009638:Norepinephrine; D014439:Tyramine