Use of a nitrogen detector for GLC determination of fluorouracil in plasma during single- and combined-agent chemotherapy.
A GLC assay for fluorouracil was developed and used to monitor plasma drug levels in patients on both single- and combined-agent chemotherapy. Fluorouracil is extracted from plasma, derivatized by flash methylation, and estimated using a thermionic nitrogen-phosphorus detector. The GLC determination was accurate at concentrations as low as Q.1 microgram/ml of human plasma. Other drugs commonly used in combination with fluorouracil did not interfere with the assay.
D000970:Antineoplastic Agents / Q000008:administration & dosage; D002849:Chromatography, Gas / Q000295:instrumentation; D004359:Drug Therapy, Combination; D005472:Fluorouracil / Q000008:administration & dosage / Q000097:blood*; D006801:Humans; D008722:Methods
D000970:Antineoplastic Agents; D005472:Fluorouracil