[Detection of trenbolone residues in meat by thin layer chromatography and fluorimetry (author's transl)].
An new method for the qualitative and quantitative determination of trenbolone residues in meat, liver and kidney is described. The analytical procedure consists of the following steps: comminution of the meat sample; homogenisation with tetrahydrofurane; liquid-liquid partition first between acetonitril and hexane then between sodium hydroxide solution and petroleum ether/benzene; chromatography on a silica gel column. The detection on the TLC-plate is performed with 366 nm ultra violet light. The quantitative determination is carried out by means of a remission spectral fluorimeter directly on the plate. The detection limit in meat extracts was 5 ppb.
D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D004963:Estrenes / Q000032:analysis*; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008460:Meat / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014204:Trenbolone Acetate / Q000032:analysis*
D004963:Estrenes; D014204:Trenbolone Acetate
Nachweis von Trenbolonrückständen in Fleisch durch Dünnschichtchromatographie und Fluorimetrie.