Device for quantitative cytochemistry: a computerized scanning microdensitometer 'grain counter'.
A computerized scanning microdensitometer and autoradiographic grain counter was able to provide quantitative data on the cytochemical final reaction product formed within a single cell and also quantitate the kinetics of its formation. Optical density and area measurements were performed on hundreds of leukocytes from slides previously stained to demonstrate any one of a variety of reactions. These included cellular glycogen, lipids, peroxidase, esterases, alkaline phosphatase, and acid phosphatase. In addition to these slide studies, chamber studies with an adapted Dvorak-Stotler Chamber allowed the measurement of enzyme kinetics within single cells.
D001345:Autoradiography / Q000295:instrumentation; D003201:Computers; D004950:Esterases / Q000032:analysis; D006003:Glycogen / Q000032:analysis; D006651:Histocytochemistry / Q000295:instrumentation*; D006801:Humans; D007962:Leukocytes / Q000032:analysis / Q000201:enzymology; D008055:Lipids / Q000032:analysis; D010544:Peroxidases / Q000032:analysis
D008055:Lipids; D006003:Glycogen; D010544:Peroxidases; D004950:Esterases