Analysis of prazosin in plasma by a sensitive high-performance liquid chromatographic-fluorescence method.
A specific high-performance liquid chromatographic-fluorescence method for the quantitative analysis of prazosin in plasma at concentrations down to 0.2 ng/ml is described. The method involves the coextraction of drug and an internal standard from alkalinized plasma followed by a simple purification step prior to evaporation and high-performance liquid chromatographic-fluorescence analysis. The method is sufficiently sensitive to allow pharmacokinetic analyses of 1-mg doses through five half-lives, with a relative standard deviation of 12%.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008722:Methods; D011224:Prazosin / Q000097:blood*; D011799:Quinazolines / Q000097:blood*; D013050:Spectrometry, Fluorescence
D011799:Quinazolines; D011224:Prazosin