title: [DNA packing in the DNP fibrils of polytene chromosomes].
abstract: Isolated polytene chromosomes stained with acridine orange were stretched with micromanipulator needles. Changes of chromosome fluorescence polarization led to the conclusions: 1) almost complete orientation of DNP fibers is attained at very low levels of chromosome stretching (3--8 times) without irreversible disruption of chromomeres; 2) internucleosomal DNA in native DNP fibers is supercoiled and makes 30 degrees angle to the fiber axis.
mesh_terms: D000166:Acridines; D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA; D003852:Deoxyribonucleoproteins; D004175:Diptera; D005454:Fluorescence Polarization; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins
chemical_list: D000166:Acridines; D003852:Deoxyribonucleoproteins; D009698:Nucleoproteins; D004247:DNA
vernacular_title: Ukladka DNK v fibrillakh DNP politennykh khromosom.