Glucose in vertebrate skeletal muscle proteins.
Neutral sugar was found in proteins extracted with acid chloroform/methanol from skeletal muscle of frog, normal and dystrophic chicken as well as in conventional preparations of frog tropomyosin and rabbit myosin. Whenever tested, the sugar content remained unaffected by two extractions with 5% trichloroacetic acid and by treatment with acid (0.1 N hydrochloric acid) or alkali (0.1 N sodium hydroxide, 60--90 min, 100 degrees C). These and other chemical properties favor the notion that sugar is present in covalent linkage to protein rather than as an attending contaminant. In every protein studied sugar profiles consisted of glucose only except rabbit myosin where it was accompanied by another sugar, presumably ribose.
D000818:Animals; D001001:Anura; D002241:Carbohydrates / Q000032:analysis; D002645:Chickens; D005947:Glucose / Q000032:analysis*; D009124:Muscle Proteins / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009137:Muscular Dystrophy, Animal / Q000378:metabolism; D009218:Myosins; D011817:Rabbits; D014335:Tropomyosin
D002241:Carbohydrates; D009124:Muscle Proteins; D014335:Tropomyosin; D009218:Myosins; D005947:Glucose