Combined enzymatic assays for 3-hydroxybutyrate and lactate: end-point and kinetic methods.
The consecutive measurement of 3-hydroxybutyrate and lactate by enzymatic end-point and rapid kinetic methods is described. Using end-point analysis within batch coefficient of variation (C.V.) ranged from 0.5 to 6.5% depending on concentration, and between batch C.V. was 1.7 to 9.0%. In the rapid kinetic method C.V. ranged from 4.2 to 10.6%. Both assays showed absolute agreement with conventional spectrophotometric methods. The rapid kinetic method yields plasma values within 15 min of blood sampling and is suitable for use in emergency situations.
D006801:Humans; D006884:Hydroxybutyrate Dehydrogenase; D006885:Hydroxybutyrates / Q000097:blood*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase; D007773:Lactates / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D006885:Hydroxybutyrates; D007773:Lactates; D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase