Comparison of the kininogenase activity of human pancreatic trypsins and porcine Kallikrein on Met-Lys-bradykinin and human plasma kininogen.
Human trypsins 1 and 2 both converted Met-Lys-bradykinin to bradykinin and released bradykinin from kininogen in human plasma as measured by bioassay with the isolated guinea pig ileum. Porcine kallikrein did not act on Met-Lys-bradykinin and released kallidin from human kininogen. Since human trypsin 1 is only partially and trypsin 2 completely inhibited by soybean trypsin inhibitor, these data show that the criterion of susceptibility to soybean trypsin inhibitor cannot be used to discriminate between trypsin and kallikrein of different species.
D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives*; D002417:Cattle; D006801:Humans; D007610:Kallikreins / Q000378:metabolism*; D007700:Kinetics; D007704:Kininogens / Q000097:blood; D008239:Lysine; D008715:Methionine; D010179:Pancreas / Q000201:enzymology*; D013045:Species Specificity; D013552:Swine; D014357:Trypsin / Q000378:metabolism*
D007704:Kininogens; C018103:bradykinin, Met-Lys-; D008715:Methionine; D007610:Kallikreins; D014357:Trypsin; D008239:Lysine; D001920:Bradykinin