[Simplified methods for the isolation and preservation of axenic Lamblia cultures].
The complexity and high cost of the existing methods of obtaining and preserving axenic cultures of Giardia (Meyer, 1970) have motivated the authors to look for new ways and means of simplifying the procedure. Solid media gave fair results which substantially simplified and accelerated the process and proved to be favourable for preserving the cultures longer without repassaging. Accordingly, the method recommended by the above authors facilitates the elimination of yeast from the cultures of Giardia in 15 to 24 days and the preservation of the axenic cultures over a period of 4 to 6 months without passaging, and within first two months at least the cultivation of new populations is guaranteed.
D000818:Animals; D003470:Culture Media; D005856:Germ-Free Life; D005872:Giardia / Q000302:isolation & purification*; D007422:Intestines / Q000469:parasitology; D011309:Preservation, Biological / Q000379:methods*; D011817:Rabbits; D013997:Time Factors
D003470:Culture Media
Uproshchennye sposoby dlia vydeleniia i sokhrameniia aksenicheskikh kul'tur liambliĭ.