Serum galactose determination with centrifugal analyzers.
I describe a rapid quantitative centrifugal analysis for galactose in blood. The technique involves galactose dehydrogenase. As compared to the manual (o-toluidine) method for galactose determination, analysis time (4 min 45 s on the GEMSAEC, 4 min 10 s on the CentificCHEM) and sample volume  (5 microliter) were considerably reduced. Sample deproteinization is eliminated. A modified commercial kit was also tested. Results by the procedure correlate well with those by the more classical methods and analytical recovery is about 100%. The method is applied to the determination of galactose in intravenous galactose tolerance tests. Results of actual tests are reported.
D001322:Autoanalysis / Q000379:methods; D002498:Centrifugation; D005691:Galactose Dehydrogenases; D005693:Galactosemias; D006505:Hepatitis / Q000097:blood; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood
D005691:Galactose Dehydrogenases