Preferential association of newly synthesized H3 and H4 histones with newly replicated DNA.
The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-density centrifugation in a gradient containing Metrizamide and 3-iodo-1,2-propanediol. Histones extracted from heavy and normal chromatin regions of the gradient were fractionated by acid-urea gel electrophoresis, and 3H/14C ratios of individual histones were compared. The results showed highly preferential association of newly synthesized H3 and H4 with newly replicated DNA.
D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004261:DNA Replication; D006657:Histones / Q000096:biosynthesis; D007065:Idoxuridine; D008239:Lysine / Q000378:metabolism
D002843:Chromatin; D006657:Histones; D004247:DNA; D008239:Lysine; D007065:Idoxuridine