Mapping of restriction sites in the transforming HpaI-E fragment of adenovirus type 5 DNA.
Adenovirus type 5 (Ad5) DNA was degraded with endo R . HpaI; the left-terminal fragment, HpaI-E has recently been shown to be the smallest segment of Ad5 DNA, that can transform non-permissive cells. This fragment was labelled at its termini by limited exonuclease III digestion followed by repair synthesis with DNA polymerase and alpha-32P-labelled deoxynucleoside triphosphates. It was then further digested with each of the restriction endonucleases HpaII, HaeIII, AluI, HinfI and TaqI; the cleavage products thus obtained were ordered into a physical map.
D000256:Adenoviridae / Q000235:genetics*; D002472:Cell Transformation, Viral; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes
D004262:DNA Restriction Enzymes