The effect of some cigarette smoke constituents and other compounds on the metabolism of benzo(a)pyrene in rabbit lung 9000 g supernatant.
1. The inhibitory effects, of a number of compounds, on the formation of two groups of benzo(a)pyrene metabolites in rabbit lung homogenates (9000 g supernatant) have been investigated. 2. The inhibitors comprised two groups: those inhibiting primarily benzo(a)pyrene hydroxylase activity, and those inhibiting the activity of the epoxide-metabolizing enzymes as well as hydroxylase activity. Phenol, 1-naphthol, nicotine and acetone belong to the former group; naphthalene, anthracene and chlorpromazine to the latter group. 3. The most active inhibitors also caused a significant decrease in the amount of benzo(a)pyrene bound covalently to tissue macromolecules.
D000096:Acetone / Q000494:pharmacology; D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism*; D002746:Chlorpromazine / Q000494:pharmacology; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D009538:Nicotine / Q000494:pharmacology; D010424:Pentobarbital / Q000494:pharmacology; D010947:Plants, Toxic; D011817:Rabbits; D012906:Smoke; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014026:Nicotiana
D001580:Benzopyrenes; D012906:Smoke; D000096:Acetone; D008748:Methylcholanthrene; D009538:Nicotine; D010424:Pentobarbital; D002746:Chlorpromazine