[Covalent C-terminal fixation of cyanogen bromide peptides in the liquid-phase-sequenator (author's transl)].
This paper describes the covalent fixation and hydrophilisation of homoserin lactone peptides enabling complete C-terminal sequencing in the squenator. Dimethylformamide, dimethylsulfoxide and 6M guanidine hydrochloride in water were used as solvents, ethylendiamine, hexamethylendiamine and histamine base as amino components. The diamine peptide derivative was reacted with the hydrophilic isothiocyanates I and IV, the fixed peptide was sequenced to the C-terminal amino acid, Histamine reacted particularly well and the program with 0.1N quadrol and the hydrophobic buffers was especially suitable for this derivative. The phenylthiohydantoin derivative of homoserine was proven in good yields. The application of this method is suggested.
D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D003488:Cyanogen Bromide; D006714:Homoserine; D007782:Lactoglobulins; D007783:Lactones; D008722:Methods; D010455:Peptides
D007782:Lactoglobulins; D007783:Lactones; D010455:Peptides; D006714:Homoserine; D003488:Cyanogen Bromide
Kovalente, C-terminale Fixierung von Bromcyanpeptiden im Flüssigphasen-Sequentor.