Synthesis of trinitrophenylaminolauric acid and the use of its glyceryl esters for assaying lipase by a spectrophotometric procedure.
Trinitrophenylaminolauric acid was synthesized from omega-aminolauric acid and trinitrobenzenesulfonic acid and then condensed with glycerol to yield mono-, di- and triacylglyceryl esters of this acid. Hydrolysis of these glycerides was followed by isolation of the yellow fatty acid with the aid of one solvent extraction step and estimating its content by spectrophotometry. This procedure was used to assay the activities of lipases from hog pancreas, rat bile, microsomes of rat brain and Rhizopus arrhizus delamar.
D000818:Animals; D001921:Brain / Q000201:enzymology; D005989:Glycerides; D007700:Kinetics; D007850:Lauric Acids / Q000138:chemical synthesis*; D008049:Lipase / Q000032:analysis*; D008861:Microsomes / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D051381:Rats; D012233:Rhizopus / Q000201:enzymology; D013053:Spectrophotometry / Q000379:methods; D013552:Swine; D014301:Trinitrobenzenes / Q000138:chemical synthesis
D005989:Glycerides; D007850:Lauric Acids; D014301:Trinitrobenzenes; C018410:trinitrophenylaminolauric acid; D008049:Lipase