Enzyme immobilization on heparin.
We describe the preparation and some of the properties of heparin-bound alpha-chymotrypsin that were obtained via activation of heparin with water-soluble carbodiimide. Immobilized enzyme has unchanged kinetic characteristics toward low-molecular- weight and macromolecular substrates. The heparin-bound enzymes could have a wide range of medical applications.
D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors; D005022:Ethyldimethylaminopropyl Carbodiimide; D006493:Heparin
D004800:Enzymes, Immobilized; D006493:Heparin; D002918:Chymotrypsin; D005022:Ethyldimethylaminopropyl Carbodiimide