A radioimmunoassay for lithocholic acid conjugates in human serum and liver tissue.
A sensitive and specific radioimmunoassay for glycine and taurine conjugates of lithocholic acid (CLCA) has been developed. 3H-glycolithocholic acid (S.A. = 17Ci/mmol) was used as tracer. Separation of free from antibody-bound bile acid was carried out using ammonium sulphate (saturated solution). The antiserum showed high specificity for both glyco and tauro conjugated lithocholate (100% cross reaction) and lithocholic acid (25% cross reaction). The sensitivity of the assay (1 pmole/tube), was adequate for measuring CLCA in peripheral blood and hepatic tissue in man.
D000918:Antibody Specificity; D006801:Humans; D008095:Lithocholic Acid / Q000032:analysis* / Q000097:blood; D008099:Liver / Q000032:analysis*; D008107:Liver Diseases / Q000378:metabolism; D008722:Methods; D011863:Radioimmunoassay
D008095:Lithocholic Acid