Carnitine-induced uptake of L-cartinine into cells from an established cell line from human heart (CCL 27).
L-Carnitine is actively transported into Girardi human heart cells, an established cell line from human heart. The present study was undertaken to investigate the effect of different concentrations of L-Carnitine in the growth medium on the rate of uptake of L-[3H] carnitine. Increasing the concentration of L-Carnitine from 2 to 100 mumol/l in the growth medium of cells, increased the rate of uptake of L-[3H] carnitine by about 50%. The maximal effect was reached after approx. 72 h incubation. The increase in rate seemed to be caused by synthesis of increased number of carriers, as judged by the increase of V with unchanged apparent Km for the transport process. This effect of L-carnitine could be inhibited by cycloheximide, indicating the dependence on intact protein synthesis. The morphology of the cells was studied by electron microscopy. No myofilaments were found, thus the cells are dedifferentiated and no longer typical muscular cells.
D001693:Biological Transport, Active / Q000187:drug effects; D002331:Carnitine / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure
D003513:Cycloheximide; D002331:Carnitine