title: DNA synthesis in subpopulations of blood mononuclear leucocytes in human subjects after vaccination against yellow fever.
abstract: After vaccination of five volunteers with yellow fever live vaccine, blood mononuclear cells were isolated and labelled with 3H-thymidine at intervals. DNA synthesis was measured by scintillation counting and autoradiography of rosetted cells. Rosetting with sheep erythrocytes (E-RFC) identified T cells, and such erythrocytes coated with IgM antibodies and complement (EAC-RFC) identified B cells and monocytes. DNA synthesis in the total mononuclear cell fraction, as well as in subfractions enriched in or deprived of E-RFC, displayed a sharp increase on day 10--11 after vaccination, remained high on day 13--14, and then returned to the prevaccination level. There was a corresponding morphological transformation, measured by size distribution and number of nucleoli per cell. The major fraction of DNA-synthesizing cells before, during and after the peak of activity was found among non-rosette-forming cells. However, during the activity peak the numbers and proportion of DNA-synthesizing E-RFC were increased while the response with regard to EAC-RFC was not obvious. Thus within a complex cellular response a transient T-cell response was identified.
mesh_terms: D000328:Adult; D001345:Autoradiography; D002466:Cell Nucleolus; D002472:Cell Transformation, Viral; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D012397:Rosette Formation; D014611:Vaccination; D014765:Viral Vaccines / Q000008:administration & dosage; D015004:Yellow Fever / Q000517:prevention & control*
chemical_list: D014765:Viral Vaccines; D004247:DNA