Preliminary studies with the sensitive cytochemical assay for parathyroid hormone.
A modification of the cytochemical assay for parathyroid hormone (PTH) is described which permits the measurement of as little as 0.1 fg ml-1. Using this assay circulating concentrations in normal humans range between 1 and 30 pg ml-1 using a bovine PTH standard. Elevated values are found in hyperparathyroidism with reduced concentrations in hypoparathyroidism. Where detectable, PTH concentrations measured by radioimmunoassay are on average fourteen-fold higher than by cytochemical assay. No cross reactivity was found with AVP, calcitonin and 1,25 dihydroxy vitamin D in concentrations normally found in health or disease.
D000328:Adult; D000368:Aged; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002118:Calcium / Q000097:blood; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D006961:Hyperparathyroidism / Q000097:blood; D007011:Hypoparathyroidism / Q000097:blood; D007686:Kidney Tubules, Distal / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000097:blood*
D010281:Parathyroid Hormone; D005954:Glucosephosphate Dehydrogenase; D002118:Calcium