Comparison of benzo[a]pyrene metabolism by human peripheral blood lymphocytes and monocytes.
Here we report a more sensitive high-pressure liquid chromatographic (HPLC) assay of benzo[a]pyrene (BP) metabolites elaborated by human monocytes and lymphocytes using only 10 X 10(6) cells for HPLC analysis. The major metabolites formed by both lymphocytes and monocytes were 3-hydroxy-BP, 9-hydroxy-BP and quinones. BP-dihydrodiols were also found by HPLC analysis, the major one being BP-7,8-dihydrodiol for both cell types. In addition, a peak slightly more polar than 9,10-dihydrodiol was formed by lymphocytes. The 7,8-diol peak was eliminated by the addition of 1,1,1-trichloropropene oxide to the incubation mixture. The presence of alpha-napthoflavone resulted in an overall decrease in metabolite production.
D001580:Benzopyrenes / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004852:Epoxy Compounds / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009281:Naphthalenes / Q000494:pharmacology
D001580:Benzopyrenes; D004852:Epoxy Compounds; D005419:Flavonoids; D006843:Hydrocarbons, Chlorinated; D009281:Naphthalenes