Malignant transformation of cryopreserved early passage syrian golden hamster cells by 2-(2-furyl)-3-(5-nitro-2-furyl)-acrylamide.
2-(2-Furyl)-3-(5-nitro-2-furyl)-acrylamide (AF-2) induced the malignant transformation of secondary cultures of Syrian golden hamster embryo cells prepared from cryopreserved primary cells. Transformed cells grew in semi-solid agar medium and formed sarcomas when inoculated subcutaneously into non-immunosuppressed suckling hamsters.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D006224:Cricetinae; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005520:Food Preservatives / Q000633:toxicity*; D005668:Furylfuramide / Q000633:toxicity*; D008647:Mesocricetus; D009368:Neoplasm Transplantation; D009581:Nitrofurans / Q000633:toxicity*; D012513:Sarcoma, Experimental / Q000139:chemically induced; D014184:Transplantation, Homologous
D005520:Food Preservatives; D009581:Nitrofurans; D005668:Furylfuramide