Demonstration of high molecular weight poly (adenosine diphosphate ribose).
An electrophoretic system was established that resolves poly(adenosine diphosphate ribose), enzymatically synthesized polymer from NAD+, by size difference of one residue on polyacrylamide gel. The existence of a polymer of at least 65 residues was demonstrated by band counting in this system. The polymer showed a heterogeneous size distribution on the electrophoregram, and the molecular weight of the largest polymer was deduced to be more than 4.5 X 10(5) daltons. The discrepancy between the size, estimated by electrophoresis, and the chain length, determined by the ratio of total radioactivity to that derived from the terminus, suggests that the polymer has a branched structure.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008099:Liver / Q000032:analysis; D008970:Molecular Weight; D009702:Nucleoside Diphosphate Sugars / Q000032:analysis*; D011064:Poly Adenosine Diphosphate Ribose / Q000032:analysis*; D051381:Rats; D013950:Thymus Gland / Q000032:analysis
D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose