Relative merits of two electrophoretic and two column-chromatographic kets for determining serum creatine kinase isoenzyme MB activity.
Prospective analysis (by use of commercially available kits) of 339 validated admission specimens from patients consecutively admitted to a cardiac monitoring unit revealed sensitivities of 68 and 63% for two electrophoretic assays (Bioware and Helena) and up to 73 and 77% for two chromatographic assays (Roche and Worthington). Specificities were 94.8, 96,6, 90.2, and 89.7% and efficiencies were 90.2, 90.7, 87.0, and 87.0, respectively. Although these results differ from early reports, they agree well with several recent studies. However, 86 (25%) of the specimens gave at least one discrepant result based on clinical evaluation of the patients, and 68 (20%) gave at least one discrepant result between methods.
D002852:Chromatography, Ion Exchange; D003402:Creatine Kinase / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D004588:Electrophoresis, Cellulose Acetate; D005069:Evaluation Studies as Topic; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D009203:Myocardial Infarction / Q000175:diagnosis; D011933:Reagent Kits, Diagnostic
D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D003402:Creatine Kinase