Isolation and partial characterization of a 67Ga-binding glycoprotein from Morris 5123C rat hepatoma.
A Glycoprotein, particularly high in tumors, has been extracted from Morris 5123C rat hepatomas and purified. The compound constitutes a major binding component for 67Ga in this hepatoma. It has a molecular weight of approximately 45,000. Its molecular weight was determined by sodium dodecyl sulfate:polyacrylamide gel electrophoresis and by Sephadex G-200 superfine gel filtration. The steps involved in its extraction and purification include ultrafiltration, gel filtration through Sephadex G-200 superfine, ion-exchange chromatography on diethylaminoethyl Sephadex A-50, and hydroxylapatite chromatography. The homogeneity of the compound was established by gel electrophoresis. The NH2-terminal residue, the percentage of nitrogen, the nonamino carbohydrate content, and the amino acid composition are reported.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D005260:Female; D005710:Gallium Radioisotopes; D006023:Glycoproteins / Q000302:isolation & purification*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D009584:Nitrogen / Q000032:analysis; D051381:Rats
D000596:Amino Acids; D002241:Carbohydrates; D005710:Gallium Radioisotopes; D006023:Glycoproteins; D009363:Neoplasm Proteins; D009584:Nitrogen