Reversible effects on cellular metabolism and proliferation by trisodium phosphonoformate.
The antiviral compound trisodium phosphonoformate (PFA), which inhibits herpesvirus multiplication by 50% at a concentration of 10 muM, did not show any effects on macromolecular synthesis and cell proliferation in HeLa and human lung cells at this concentration. At the high concentration of 2 mM, PFA reduced DNA synthesis to 50% after 1 h of treatment, whereas no effects could be seen on RNA and protein synthesis. Treatment for 24 h with 1 mM PFA inhibited both DNA synthesis and cell proliferation to 50%. The inhibition of DNA synthesis and cell proliferation at 10 mM PFA was rapidly reversed by removing the drug from the cells.
D000998:Antiviral Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002477:Cells / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005561:Formates / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013997:Time Factors
D000998:Antiviral Agents; D005561:Formates; D009943:Organophosphorus Compounds; D012313:RNA; D004247:DNA