[Effect of medium components on the metabolism of Brevibacterium ammoniagenes-producer of 5'-inosinic acid].
The effect of different carbon and nitrogen sources on the biosynthesis of 5'-inosinic acid (5'-IMP) by the culture Brevibacterium ammoniagenes 225-5 was studied. With respect to the yield of 5'-IMP, glucose was found to be the best carbon source and urea--the best nitrogen source. The proper ratio of concentrations of carbon and nitrogen sources in the nutrient medium was very important for a high 5'-IMP accumulation. The study of the effect of phosphate and magnesium concentrations on 5'-IMP biosynthesis demonstrated that their high concentrations were needed for intensive 5'-IMP salvage-synthesis from hypoxanthine and that they did not influence the de novo synthesis of hypoxanthine.
D001951:Brevibacterium / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D007042:Hypoxanthines / Q000378:metabolism; D007291:Inosine Monophosphate / Q000096:biosynthesis*; D007292:Inosine Nucleotides / Q000096:biosynthesis*; D008274:Magnesium / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D003470:Culture Media; D007042:Hypoxanthines; D007292:Inosine Nucleotides; D010710:Phosphates; D007291:Inosine Monophosphate; D014508:Urea; D008274:Magnesium; D005947:Glucose
Vliianie komponentov sredy na metabolizm kul'tury Brevibacterium ammoniagenes-produtsenta 5'-inozinovoĭ kisloty.