The composition, structure and origin of proteose-peptone component 5 of bovine milk.
Proteose-peptone component 5 has been isolated from bovine milk. Molecular weight values within the range 12000--13500 were obtained by sedimentation equilibrium, dodecylsulphate/polyacrylamide gel electrophoresis and gel filtration in urea-containing buffers. A dansylation procedure showed that the sequence Arg-Glu occupied the N-terminal position while hydrazinolysis revealed C-terminal lysine. The latter was confirmed by experiments with carboxypeptidases B and C which indicated that a mixture of molecules was present, about 80% of which had a C-terminal sequence -(Ala-Met)-Ala-Pro-Lys while about 20% had an additional -His-Lys in the terminal position. These results, together with data on the overall composition, showed that this component of the proteose-peptone fraction of milk corresponded to a mixture of molecules representing residues 1--105 and 1--107 of the beta-casein molecule, a finding that was confirmed by peptide mapping. This demonstration that proteose-peptone components correspond to the N-terminal portions of the beta-casein molecule while the gamma-caseins represent the matching C-terminal portions provides strong evidence in favour of a proteolytic mechanism for the formation of these substances in vivo and in vitro.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002268:Carboxypeptidases / Q000378:metabolism; D002364:Caseins / Q000302:isolation & purification; D002417:Cattle; D005260:Female; D007700:Kinetics; D008892:Milk / Q000032:analysis; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis
D000596:Amino Acids; D002364:Caseins; D010446:Peptide Fragments; C018135:proteose-peptone; D002268:Carboxypeptidases