Effect of topical acid on duodenal pepsinogen secretion in the rat.
The effect of topical acid on duodenal pepsinogen secretion was studied in the anesthetized rat. Perfusion of a 5-cm segment of the proximal duodenum with normal saline or buffered saline (pH 7.2 or 6.0) elicited no detectable pepsinogen response. Perfusion with 10, 25, and 100 mN HCl resulted in a graded increase in pepsinogen output. Acetylcholine bromide, 500 microgram/ml, in buffered saline, pH 7.2, also stimulated pepsinogen secretion. The pepsinogen response to 100 mN HCl and to acetylcholine was completely abolished by atropine. Secretin, 2 units/kg, did not alter pepsinogen output during perfusion with buffered saline or acid, while secretin, 75 units/kg, increased pepsinogen output. These observations suggest that topical acid stimulates duodenal pepsinogen secretion through a cholinergic reflex and that secretin is not a significant stimulant of duodenal pepsinogen secretion in the rat within the dose range employed (1--2 units/kg).
D000109:Acetylcholine / Q000494:pharmacology; D000287:Administration, Topical; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004386:Duodenum / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D006851:Hydrochloric Acid / Q000008:administration & dosage / Q000494:pharmacology*; D007441:Intubation, Gastrointestinal; D008297:Male; D010275:Parasympathetic Nervous System / Q000502:physiology; D010435:Pepsinogens / Q000378:metabolism*; D051381:Rats; D012018:Reflex; D012633:Secretin / Q000494:pharmacology; D012636:Secretory Rate / Q000187:drug effects; D013268:Stimulation, Chemical
D010435:Pepsinogens; D012633:Secretin; D001285:Atropine; D000109:Acetylcholine; D006851:Hydrochloric Acid