Regional levels of histamine in rat brain after microwave irradiation: evidence for artifacts in the enzymatic--isotopic assay.
Histamine levels in the brain, measured fluorimetrically, were similar for rats killed by decapitation and microwave irradiation. However, enzymatic--isotopic assay yields 5--20 times higher value for microwave irradiation versus decapitation. Thin layer chromatography of the methylated products in the enzymatic--isotopic assay indicated the formation of artifacts following microwave irradiation. The results strongly suggest that a combination of microwave irradiation and enzymatic--isotopic assay is not suitable for histamine analysis in the rat brain.
D000818:Animals; D001923:Brain Chemistry / Q000528:radiation effects*; D005470:Fluorometry; D006632:Histamine / Q000032:analysis* / Q000528:radiation effects; D008297:Male; D008722:Methods; D008745:Methylation; D008872:Microwaves; D051381:Rats
D006632:Histamine