title: Quantitative analysis of RNA, glycogen and nucleotides from different developmental stages of Ascaris lumbricoides (var. suum).
abstract: Cold acid phenol extractions (Kirby, K.S. (1968) in Methods in Enzymology, Vol. XII B, pp. 87--99, Academic Press, New York) of homogenates from oocytes, zygotes and larval stages of Ascaris lumbricoides yield predominantly glycogen, some glycogen . phosphate complexes and guanine nucleotides, but only small amounts of RNA. Oocytes contain 10.0 pg of extractable 19 S and 26 S rRNA and 1.1 pg 4 S and 5 S RNA per cell. During the maturation of the oocyte to the zygote stage, the amount of 19 S and 26 S rRNA increases by 57%, and that of 4 S and 5 S RNA by 130%. Larval stages contain 62% more 19 S and 26 S rRNA, and 47% more 4 S and 5 S RNA than zygotes. The amount of the extractable glycogen decreases by about 80% and nucleotides by about 70% from oogenesis to the larval stage. The Kirby extract from homogenates of spermatids contains only traces of glycogen, glycogen . phosphate complexes and nucleotides, and 115 times less RNA per spermatid than oocytes. The nucleotide pool sizes of oocytes, zygotes and larval stages were determined; the pools consist of 71--88% guanine nucleotides. Spermatids contain only adenine nucleotides and an unidentified, nucleotide-like compound.
mesh_terms: D000818:Animals; D001200:Ascaris / Q000502:physiology*; D005260:Female; D006003:Glycogen / Q000378:metabolism*; D007814:Larva; D008297:Male; D008675:Metamorphosis, Biological; D009865:Oocytes / Q000378:metabolism; D010053:Ovary / Q000378:metabolism / Q000648:ultrastructure; D012313:RNA / Q000378:metabolism*; D012265:Ribonucleotides / Q000378:metabolism*; D013087:Spermatids / Q000378:metabolism
chemical_list: D012265:Ribonucleotides; D012313:RNA; D006003:Glycogen