Alkaline phosphatase from Bacillus licheniformis. Solubility dependent on magnesium, purification and characterization.
The membrane-associated alkaline phosphatase (orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1) from Bacillus licheniformis MC14, a facultative thermophile, was purified to homogeneity in buffer containing 0.2 M Mg2+. The alkaline phosphatase purified in this manner is insoluble upon removal of the magnesium by dialysis. This insoluble alkaline phosphatase has been characterized and compared to the previously purified heat-solubilized enzyme (Hulett-Cowling, F.M. and Campbell, L.L. (1971) Biochemistry 10, 1364--1371).
D000469:Alkaline Phosphatase / Q000302:isolation & purification*; D001407:Bacillus / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology; D006358:Hot Temperature; D007700:Kinetics; D008274:Magnesium; D008970:Molecular Weight; D012995:Solubility
D000469:Alkaline Phosphatase; D008274:Magnesium