Immune adherence: a quantitative and kinetic analysis.
Quantitative and kinetic analysis of the immune-adherence reaction (IA) between C3b fragments and IA receptors as an agglutination reaction is difficult. Analysis is possible, however, by use of radio-iodinated bovine serum albumin as antigen at low concentrations (less than 200 ng/ml) and optimal concentration of antibody to avoid precipitation of antigen--antibody complexes with human erythrocytes without participation of complement. Antigen and antibody are reacted at 37 degree C, complement is added, the mixture incubated and human erythrocytes added; after further incubation, ice-cold EDTA containing buffer is added and the erythrocytes centrifuged and assayed for radioactivity. Control cells reacted with heated guinea pig serum retained less than 5% of the added radioactivity. The method facilitates measurement of IA reactivity and permits more detailed analysis of the mechanism underlying the reaction.
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003165:Complement System Proteins; D007104:Immune Adherence Reaction; D007700:Kinetics; D012710:Serum Albumin, Bovine / Q000276:immunology; D013997:Time Factors
D000906:Antibodies; D000936:Antigen-Antibody Complex; D012710:Serum Albumin, Bovine; D003165:Complement System Proteins