title: Gas-liquid chromatographic determination of mono- and diiodotyrosines in serum.
abstract: A sensitivie, reliable gas-chromatographic assay for monoiodotyrosine and diiodotyrosine in human serum is reported. The oxazolidinone-heptafluorobutyric anhydride derivatives allow the quantitation of both compounds in the linear range of 0.2 to 7.6 mg/L of serum. Analytical recovery averaged 88%, and mean accuracy and within-run precision were 98 and 2%, respectively. Concentrations of monoiodotyrosine in serum as low as 20 microgram/L and of diiodotyrosine as low as 100 microgram/L can be detected. Normal serum contains no detectable concentration of either compound, but the method is applicable as a diagnostic tool in the early prediction of thyroid disease. Both compounds were detected in the serum of a hypothyroid subject whose normal thyroid hormone concentrations were being maintained by therapy with desiccated thyroid extract.
mesh_terms: D002087:Butyrates; D002849:Chromatography, Gas / Q000379:methods; D004105:Diiodotyrosine / Q000031:analogs & derivatives / Q000097:blood*; D005466:Fluorocarbons; D006801:Humans; D007037:Hypothyroidism / Q000097:blood; D007202:Indicators and Reagents; D007470:Monoiodotyrosine / Q000031:analogs & derivatives / Q000097:blood*; D010080:Oxazoles
chemical_list: D002087:Butyrates; D005466:Fluorocarbons; D007202:Indicators and Reagents; D010080:Oxazoles; C007924:heptafluorobutyric anhydride; D004105:Diiodotyrosine; D007470:Monoiodotyrosine