Determination of indicine N-oxide and indicine in plasma and urine by electron-capture gas-liquid chromatography.
A sensitive and specific method is described for the quantitative analysis of indicine N-oxide and indicine in plasma and urine. Indicine N-oxide is reduced to the tertiary amine with zinc--acetic acid (plasma) or zinc--ammonium chloride (urine). The indicine is derivatized with pentafluoropropionic anhydride to provide electron-capture detection. Assay of rabbit and human plasma and urine samples can detect 100 ng/ml (plasma) and 200 ng/ml (urine), and the method was applied to the analysis of indicine N-oxide and indicine in rabbits and one patient following administration of indicine N-oxide.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D002853:Chromatography, Liquid; D003497:Cyclic N-Oxides / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D008832:Microchemistry; D011763:Pyrrolizidine Alkaloids / Q000032:analysis* / Q000097:blood / Q000652:urine; D011817:Rabbits
D003497:Cyclic N-Oxides; D011763:Pyrrolizidine Alkaloids; C018336:indicine