The effect of dietary protein levels in isocaloric diets on the composition of avian pancreatic juice.
A permanent indwelling cannula was placed in the main pancreatic duct of 10-week-old commercial strain male chickens. The birds were fed isocaloric corn-soybean meal diets containing either 11.5% or 18.5% crude protein for two 5-day periods in a reversal experimental design. Irrespective of diet, chickens consumed more feed and secreted more pancreatic juice during the daytime than during the nighttime (P less than .01). The concentrations of crude protein and chloride ion and the activities of trypsin, chymotrypsin, and amylase in the pancreatic juice were the same for the daytime and nighttime periods. Significant differences were observed between the two collection periods for the total quantities of these materials secreted (P less than .01). Although the chickens consumed equal amounts of the two rations, trypsin and chymotrypsin responded to the dietary change. Greater proteolytic enzyme activities were found in the pancreatic juice as a response to eating the 18.5% protein diet. The chickens remained in excellent condition throughout the experiment, the cannulas remained unobstructed, and the pancreata secreted up to seven weeks with no cessation of flow.
D000681:Amylases / Q000032:analysis; D000821:Animal Feed; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D002712:Chlorides / Q000032:analysis; D002918:Chymotrypsin / Q000032:analysis; D004044:Dietary Proteins / Q000378:metabolism*; D008297:Male; D010189:Pancreatic Juice / Q000201:enzymology*; D014357:Trypsin / Q000032:analysis
D002712:Chlorides; D004044:Dietary Proteins; D000681:Amylases; D002918:Chymotrypsin; D014357:Trypsin