The use of deep frozen, stored bovine blood for in vitro feeding of tsetse flies.
A method of blood preparation is described which resulted in the successful rearing of Glossina p. palpalis with in vitro feeding. Cells of defibrinated bovine blood were washed in 0.89% NaCl solution prior to deep-freezing. Cells and serum were stored (--28 degrees C) for up to four months. For feeding, both components were mixed in the volumetric proportion 1 : 1. Adenosine triphosphate was added at a concentration of 10-3 M to stimulate uptake of blood. Survival rate and mean weight of puparia remained constant over three generations while productivity increased.
D000255:Adenosine Triphosphate; D000821:Animal Feed; D000818:Animals; D000830:Animals, Laboratory; D001769:Blood; D001947:Breeding; D002417:Cattle; D005615:Freezing; D011679:Pupa / Q000254:growth & development; D014370:Tsetse Flies / Q000254:growth & development*
D000255:Adenosine Triphosphate