Identification and determination of 25-hydroxyvitamin D3 in the blood and liver of vitamin D-deficient rats irradiated with ultraviolet light.
The intestinal calcium transport activity and serum calcium and phosphorous concentrations of vitamin D-deficient rats were increased by irradiation with an ultraviolet (UV) lamp. The existence of 25-hydroxyvitamin D3(25-OH-D3) in their bloods and livers was physicochemically confirmed by high-performance liquid chromatography (HPLC), gas-liquid chromatography (GLC) and mass fragmentography, whereas the compound could not be detected in the tissues of non-irradiated rats. The results strongly suggested that vitamin D3 in vivo generated in irradiated rat skin might be normally metabolized and utilized to prevent rickets. The level of 25-OH-D3 in the tissues was determined by a HPLC method.
D000818:Animals; D001692:Biological Transport; D001769:Blood / Q000528:radiation effects*; D002118:Calcium / Q000378:metabolism; D006887:Hydroxycholecalciferols / Q000097:blood / Q000378:metabolism / Q000528:radiation effects*; D008099:Liver / Q000528:radiation effects*; D008297:Male; D010758:Phosphorus / Q000097:blood; D051381:Rats; D012279:Rickets / Q000378:metabolism; D012867:Skin / Q000528:radiation effects; D014466:Ultraviolet Rays; D014808:Vitamin D Deficiency / Q000378:metabolism*
D006887:Hydroxycholecalciferols; D010758:Phosphorus; D002118:Calcium