A rapid method for measuring the phospholipid synthetic activity of incubated lymphocytes.
A rapid and simple method for estimating the phospholipid synthetic activity of incubated lymphocytes by a Florisil column technique following the uptake of 14C-oleic acid was established. Stimulation of phospholipid synthesis by PHA and inhibitions caused by Tween 20 or Tween 80 and heating were evaluated easily with this method.
D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods; D006358:Hot Temperature; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D051381:Rats
D009829:Oleic Acids; D010743:Phospholipids; D010835:Phytohemagglutinins; D011136:Polysorbates