Fluoremetric determination of methylergonovine maleate and ergonovine maleate in tablets and injections.
A column cleanup, followed by a shakeout and fluorometric determination, has been developed to determine methylergonovine maleate and ergonovine maleate in tablets and injections. The ether eluate is extracted with tartaric acid solution. An aliquot is diluted with sodium tartrate solution, and this solution is excited at 325 nm. The resulting fluorescence is measured at 432 nm. Results obtained using this method compare favorably with those from the official NF XIV procedure and a semiautomated procedure. Recoveries of 100 and 99% were obtained from one sample each of spiked excipient tablets of methylergonovine maleate and ergonovine maleate, respectively.
D004874:Ergonovine / Q000031:analogs & derivatives* / Q000032:analysis; D007267:Injections; D012996:Solutions / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013607:Tablets
D012996:Solutions; D013607:Tablets; D004874:Ergonovine