Incorporation of [18O]water in the formation of p-hydroxybenzyl alcohol by the p-cresol methylhydroxylase from Pseudomonas putida.
In the hydroxylation of the methyl group of p-cresol by an enzyme from Pseudomonas putida the oxygen atom is derived from water. Although a second reaction by the same enzyme converts the product, p-hydroxybenzyl alcohol, into the aldehyde, the alcohol is an enzyme-free intermediate.
D001592:Benzyl Alcohols / Q000378:metabolism*; D001593:Benzyl Compounds / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D003408:Cresols; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*; D014867:Water / Q000378:metabolism*
D001592:Benzyl Alcohols; D001593:Benzyl Compounds; D003408:Cresols; D014867:Water; C018966:4-hydroxybenzyl alcohol; D006899:Mixed Function Oxygenases