Instability of the tetrathionate respiratory chain of Citrobacter freundii.
The formate--tetrathionate reductase redox pathway of Citrobacter freundii is associated with the cytoplasmic membrane fraction. A high concentration of phosphate in the buffer used for cell disintegration assists in the preparing of membrane particles capable of efficient tetrathionate respiration. A part of this effect at least may be attributed to the high ionic strength of the buffer.
D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D002954:Citrobacter / Q000201:enzymology / Q000378:metabolism*; D005561:Formates / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D013776:Tetrathionic Acid / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism*
D005561:Formates; D013885:Thiosulfates; D013776:Tetrathionic Acid; D010088:Oxidoreductases