Quantitation of dexamethasone in biological fluids using high-performance liquid chromatography.
A sensitive and specific method for the quantitation of dexamethasone in plasma and urine is described. The specificity of the method is obtained using adsoprtion chromatography on a high-performance liquid chromatograph. The dexamethasone is detected with a variable-wavelength UV detector. An internal standard technique is used for quantitation of dexamethasone with a minimum sensitivity of 15 ng. Preliminary results of the application of the method to pharmacokinetic studies of dexamethasone in humans are reported.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003907:Dexamethasone / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans
D003907:Dexamethasone