[Proposed new method of separation of Hb A2 for standardization of the screening of microcythemias].
The AA. suggest a dose-method of Hb A2, which implies the adoption of tris-EDTA-Glycin tampon with a different concentration anode-cathode; the electrophoretic bands, obtained by cellulose acetate electrophoresis, are eluted in the same tampon, but it is suggested to use 15 cc of tampon for A fraction elution and 1.5 cc for A2 fraction elution. The lecture must be done by spectrophotometry to 415 nm. By using this method the normal values of Hb A2 ranges from 1.7% to 3.5% and so there isn't any confusion with pathologic values. The AA. compare this method with that recommend a densitometric lecture of the strips and with those that imply the adoption of cromathographic columns, and they underline its advantages. In conclusion, the AA. suggest the adoption of an unified method for microcytemic screening that could be used in all laboratories.
D004913:Erythrocytes, Abnormal; D006402:Hematologic Diseases / Q000175:diagnosis*; D006441:Hemoglobin A / Q000302:isolation & purification*; D006443:Hemoglobin A2 / Q000302:isolation & purification*; D006801:Humans; D008403:Mass Screening / Q000592:standards; D013789:Thalassemia / Q000175:diagnosis
D006441:Hemoglobin A; D006443:Hemoglobin A2
Proposta di un nuovo metodo di separazione dell'Hb A2 per l'unificazione dello screening delle microcitemie.