[Fragmentation of human adenovirus type 1 DNA by the specific endonucleases R. SaLI, R. EcoRI and R. BamHI].
The restricting endonuclease R. EcoRI hydrolyses human adenovirus type I (Adl) DNA with formation of three fragments with molecular weights of 17.5 x 10(6), 3.7 x 10(6), and 1.55 x 10(6) dalton. Under the effect of R. SaLI restrictase DNA Adl disintegrates into 5 fragments with molecular weights: 8.0 x 10(6), 6.0 x 10(6), 4.6 x 10(6), 4.0 x 10(6), and 0.2 x 10(6) dalton. Treatment of DNA with R. BamHI produces 4 fragments with molecular weights 10.0 x 10(6), 6.8 x 10(6), 4.0 x 10(6), and 2.2 x 10(6) dalton. Fragments of R. SaLI and R. BamHI form sequences BECAD and ACBD, respectively.
D000260:Adenoviruses, Human / Q000032:analysis*; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D005814:Genes, Viral; D006868:Hydrolysis
D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Fragmentatsiia DNK adenovirusa cheloveka tipa 1 spetsificheskimi éndonukleazami R. SaLI, R. EcoRI i R. BamHI.