Long-term perfusion of human corneas.
In twelve isolated human corneas (cadaver time 2 to 16 hr) stromal thickness could be maintained within 0.50 to 0.66 mm up to 20 hr by the use of an endothelial bathing medium consisting of glutathione-bicarbonate Ringer's solution, HEPES-buffer, and 20% human serum. During the first 3 to 5 hr of perfusion, temperature reversal occurred in all corneas. Wound healing processes were observed in nine corneas throughout the experiments after scraping off a 2.5 mm area of central epithelium, which was kept in a moist saline-saturated atmosphere.
D003315:Cornea / Q000166:cytology / Q000502:physiology* / Q000503:physiopathology; D004727:Endothelium / Q000166:cytology; D006801:Humans; D010477:Perfusion; D014945:Wound Healing