Difference in resistance of subunits A and B of Vibrio cholerae toxin (choleragen) to treatment with pronase.
Disc electrophoresis in sodium dodecyl sulphate, performed on choleragen after incubation with pronase, only showed the band corresponding to the B subunit, while the A subunit was lost. When examined in immunodiffusion, the digested choleragen was still able to precipitate with specific antibodies. On the other hand, toxicity was considerably reduced.
D002772:Cholera Toxin / Q000378:metabolism*; D006868:Hydrolysis; D046911:Macromolecular Substances; D011402:Pronase / Q000378:metabolism*
D046911:Macromolecular Substances; D002772:Cholera Toxin; D011402:Pronase