Biochemical and immunologic techniques for acid phosphatase measurement in the diagnosis of prostate cancer.
A counter immunoelectrophoresis procedure for the measurement of acid phosphatase is described. Acid phosphatase was purified from human prostate. A monospecific antiserum to the purified enzyme was produced in rabbits. With the specific antiserum and a sensitive chemical staining technique, a counter immunoelectrophoresis assay was developed for detection of serum prostate acid phosphatase. The isoenzymes of serum prostate acid phosphatase were also studied by an isoelectric focusing technique.
D000135:Acid Phosphatase / Q000032:analysis*; D003378:Counterimmunoelectrophoresis; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000201:enzymology
D007527:Isoenzymes; D000135:Acid Phosphatase