Quantitative analysis of propranolol and metabolites by a gas chromatograph mass spectrometer computer technique.
A quantitative assay for urinary propranolol and six of its unconjugated metabolites by a gas chromatography mass spectrometry computer system has been developed. Conjugated metabolites are also determined following enzyme hydrolysis. These compounds are separated from murine by Amberlite XAD-2 resin column chromatography and are converted to methyl esters and trifluoroacetyl derivatives. Each substance is quantitated from reconstructed ion chromatograms using a specific mass or sum of masses for each compound by comparing its area with that of an appropriate internal standard. The minimum detectable concentrations are in the range of 50--100 ng ml-1. The calibrations are linear from 0.10--15.0 microgram ml-1 with recoveries ranging from 62--91%. This procedure yields a high degree of specificity, selectivity and reproducibility, as demonstrated by the analyses of urine from dogs receiving single 40 mg doses of propranolol orally.
D000818:Animals; D002849:Chromatography, Gas; D003201:Computers; D004285:Dogs; D006868:Hydrolysis; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D011433:Propranolol / Q000302:isolation & purification / Q000378:metabolism / Q000652:urine*; D014269:Trifluoroacetic Acid
D007477:Ions; D011433:Propranolol; D014269:Trifluoroacetic Acid