[Structural peculiarities of the nucleic factor from ascitic fluid of tumors].
Several characteristics of the nucleic factor of tumors which is thought to play an important role in suppression of tumor immunity was investigated. This nucleic factor differs from native double-stranded DNA of eukaryotic nuclei by base composition (A: T: G : C = 1: 1.44 : 0.91: 0.69); its affinity to GAP and hyperchromic effect were lower than those in native DNA. Heating above 80 degrees in 1 N KOH destroyed the nucleic factor to fragments that could not be detected in 8% PAK electrophoresis. The results of this and previous reports lead us to a hypothesis that the nucleic factor is a DNA molecule consisting of single- and double-stranded regions, as well as short insertions of RNA. The double-stranded regions in this molecule possess non-parrallel breaks.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D002467:Cell Nucleus / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism; D004277:DNA, Single-Stranded; D004591:Electrophoresis, Polyacrylamide Gel; D011687:Purines / Q000032:analysis; D011743:Pyrimidines / Q000032:analysis
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D011687:Purines; D011743:Pyrimidines
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