title: Dextran/glucan binding by Streptococcus mutans: the role of molecular size and binding site in agglutination.
abstract: 1) S. mutans strains of serotypes a, d and g were strongly agglutinated with soluble glucans and dextran T2000. Homologous glucan did not in all cases produce agglutination. 2) The quantity of low molecular weight dextrans bound (T20 and T70) does not correspond to the agglutination induced by glucan or T2000. 3) The agglutination and binding of high molecular weight glucan by B13 cells was sensitive to heat, trypsin, dextranase, EDTA, SDS and urea, whereas no inhibition of binding of T20 and T70 was seen. 4) Pretreatment of B13 cells with anti-d, or anti-glucan sera, or Con A, RCA I, or RCA II completely inhibited agglutination by T2000 and caused a significant reduction of the binding of glucan. No reduction in the binding of T20 and T70 occurred. 5) An agglutination-negative mutant was agglutinated by sucrose but not by T2000 or high molecular weight glucan. It bound normal levels of T20 and T70. 6) The results indicate that B13 cells possess multiple glucan binding sites and that the site responsible for agglutination consists of both polysaccharide and protein. 7) Inhibition studies on agglutination and adherence using B13 cells indicate that the two processes involve different mechanisms.
mesh_terms: D000907:Antibodies, Bacterial; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D003911:Dextrans / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism*; D037102:Lectins / Q000494:pharmacology; D008970:Molecular Weight; D009154:Mutation; D013295:Streptococcus mutans / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D000907:Antibodies, Bacterial; D003911:Dextrans; D005936:Glucans; D037102:Lectins