Isolation of heavy satellite deoxyribonucleic acid from sea-urchin spermatozoa.
Two different methods were used to isolate high-molecular-weight heavy satellite DNA from the spermatozoa of the sea-urchin Lytechinus variegratus. Purification of satellite rDNA (ribosomal DNA) by selective denaturation of the spermatozoal DNA followed by a separation of the native and denatured DNA in an aqueous mixture of poly(ethylene glycol)/dextran is affected by the molecular weight of the spermatozoal DNA. Enrichment of satellite rDNA by selective precipitation of main-band DNA by poly-L-lysine is cumbersome but more suitable.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000302:isolation & purification*; D004276:DNA, Satellite / Q000302:isolation & purification*; D008297:Male; D009691:Nucleic Acid Denaturation; D011092:Polyethylene Glycols; D011107:Polylysine; D012617:Sea Urchins / Q000032:analysis*; D013094:Spermatozoa / Q000032:analysis*
D004276:DNA, Satellite; D011107:Polylysine; D011092:Polyethylene Glycols; D004247:DNA