A radioimmunoassay for chicken avidin. Comparison with a [14C]biotin-binding method.
A double-antibody solid-phase radioimmunoassay for chicken avidin is reported. Avidin was labelled with 125I by the chloramine-T method. The bound and free avidin were separated with a second antibody bound to a solid matrix. In the logit-log scale the standard curve was linear from 1-2 to 100-200ng of avidin/ml. Cross-reaction of ovalbumin was less than 0.015%. Saturation of biotin-binding sites of avidin with an excess of biotin decreased radioimmunoassay values by about 15%. Recovery studies indicated that avidin can be assayed from all chicken tissues studied with radioimmunoassay, whereas the [14C]biotin/bentonite method gave poor recoveries for avidin in the liver and kidney. Radioimmunoassay and the [14C]biotin/bentonite method gave similar concentrations for oviduct avidin.
D000818:Animals; D001360:Avidin / Q000032:analysis*; D001546:Bentonite; D001710:Biotin; D002645:Chickens; D005260:Female; D010047:Ovalbumin / Q000031:analogs & derivatives*; D010057:Oviducts / Q000032:analysis; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods; D014018:Tissue Distribution
D001546:Bentonite; D001360:Avidin; D001710:Biotin; D010047:Ovalbumin