[Amino oxyadsorbents. New type of adsorbents for affinity chromatography: purification of tRNA-methylases from rat nephron on aminooxybutylcellulose with immobilized tRNA].
A new type of sorbents for affinity chromatography is suggested and used to purify tRNA methylases. tRNA was immobilized on aminooxybutylcellulose via the oxidized 3'-end. In order to bind other enzymes specific for nucleic acids in general, e. g. nucleases, and to achieve a higher degree of purification the crude enzyme preparation was treated with rRNA immobilized on aminooxybutycellulose. The sequential application of two sorbents mentioned allows to get an approximately two hundred fold purification of total tRNA methylases. In a separate experiment the possibility of individual tRNA methylase fractionation by means of elution with a NACl gradient was shown; the degree of purification for some methylases was more than a thousand fold.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D009399:Nephrons / Q000201:enzymology*; D012343:RNA, Transfer; D051381:Rats; D012359:tRNA Methyltransferases / Q000302:isolation & purification*
D012343:RNA, Transfer; D012359:tRNA Methyltransferases
Aminooksiadsorbenty. 8. Novyĭ tip sorbentov dlia affinnoĭ khromatografii: ochistka tRNK-metilaz nefromy krysy na aminooksibytiltselliuloze s immobilizovannoĭ tRNK.