title: Hairy cell leukemia: enzyme-histochemical and ultrastructural investigation of one case.
abstract: The investigation was carried out on blood smears, bone marrow aspirates, one lymph node biopsy, and the surgically removed spleen of a 53-year-old man with hairy cell leukemia. In the blood smears stained with May-Grünwald-Giemsa, 60 to 70% of the hairy cells contained tubular inclusions that corresponded to the ribosome-lamella complexes demonstrated at electron microscopy. In blood smears, imprints and cryostatic sections of the lymph node and of the spleen, hairy cells revealed tartrate-resistant acid phosphatase, beta-glucuronidase and adenosine-triphosphatase activity. In the spleen neutral esterase and alkaline phosphatase demonstrated the numerical increase of the histiocytes, which ultrastructurally displayed phagocytic activity. The presence in the spleen of pseudosinuses lined by hairy cells was confirmed by electron microscopy as well as by cytoenzymology.
mesh_terms: D001773:Blood Cells / Q000648:ultrastructure; D001853:Bone Marrow / Q000648:ultrastructure; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000201:enzymology* / Q000648:ultrastructure; D008198:Lymph Nodes / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D013154:Spleen / Q000648:ultrastructure