[Comparative biochemical and histological studies of the effect of magnesium and profound hypothermia on the preservation of the isolated perfused rat liver (author's transl)].
In order to determine, if magnesium aspartate has the same favorable effect on the liver as on the arrested heart, the isolated rat liver preperfused with isotonique Mg-aspartate or saline solution 15 minutes and stored 1 hour at 36 degrees C or 12 hours at 36 degrees C has been reperfused 150 minutes at 37 degrees C. During the storage the concentrations of ATP and ADP decrease significantly whereas AMP, lactate and beta-hydroxybutyrate increase remarkably, accompanied by a pronounced breakdown of glycogen. Upon reperfusion a de novo synthesis of ATP and ADP is resumed. Lactate, beta-hydroxybutyrate and AMP approach normal values whereas a resynthesis of glycogen is missing. A focal distribution of well preserved and disintegrated hepatocytes is being observed and seems rather to be due microcirculatory disorders. No conclusive evidence of a protective Mg-effect as observed in induced cardiac arrest has been obtained. Several concentrations of Mg-aspartate should be studied, in order to elucidate the different acting mechanism of Mg++ on the two organs.
D000244:Adenosine Diphosphate / Q000096:biosynthesis; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D007036:Hypothermia, Induced; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008112:Liver Glycogen / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D051381:Rats
D008112:Liver Glycogen; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D008274:Magnesium
Etude comparative biochimique et histologique de l'action du magnésium et de l'hypothermie profonde sur la conservation du foie de rat isolé.