Isolation and characterization of a novel ganglioside, monosialosyl pentahexaosyl ceramide from human brain.
A novel monosialosyl ganglioside was isolated from human brain in a yield of 2.1 nmol of the lipid-bound sialic acid per g of wet weight (0.09% of total lipid-bound sialic acid). The ganglioside was resistant to the action of Vibrio cholerae neuraminidase. By treatment with N-acetylhexosaminidase, methylation analysis, CrO3 oxidation, and partial acid hydrolysis, the structure of this ganglioside was determined to be; GalNAc(beta, 1-4)Gal(beta, 1-3)GalNAc(beta, 1-4)Gal(beta, 1-4)Glc(beta, 1-1)ceramide. (formula: see text). The treatment of the ganglioside with 1 N formic acid at 80 degrees C for 2 h produced mainly gangliotetraosyl ceramide, suggesting that the terminal N-acetylgalactosamine was more labile on formic acid treatment than galactose at nonreducing terminal.
D000328:Adult; D001923:Brain Chemistry; D002241:Carbohydrates / Q000032:analysis; D002518:Ceramides / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005732:Gangliosides / Q000302:isolation & purification; D006801:Humans; D008745:Methylation; D008968:Molecular Conformation; D012794:Sialic Acids / Q000032:analysis
D002241:Carbohydrates; D002518:Ceramides; D005227:Fatty Acids; D005732:Gangliosides; D012794:Sialic Acids