Effect of antibody avidity on the induction of renal injury in anti-glomerular basement membrane nephritis.
Antibodies to glomerular basement membrane (GBM) of the rat could be fractionated according to their avidity by elution from trypsin-digested GBM (bound to Sepharose) with increasing concentrations of KSCN. The percentage of kidney-fixing antibody in each fraction and the degree of proteinuria induced as determined 24 h after injection increased with the avidity of the antibody fraction when equal doses were administered.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000915:Antibody Affinity; D001485:Basement Membrane / Q000276:immunology; D007678:Kidney Glomerulus / Q000276:immunology*; D008297:Male; D009393:Nephritis / Q000276:immunology*; D011507:Proteinuria / Q000209:etiology; D051381:Rats
D000906:Antibodies