Biological monitoring of exposure to styrene by analysis of combined urinary mandelic and phenylglyoxylic acids.
Human volunteers were exposed in an experimental chamber to styrene (4 or 8 hours at 40 to 200 ppm) in order to obtain a quantitative relationship between exposure and urinary elimination of the metabolites mandelic and phenylglyoxylic acids (MA and PGA). For the analysis of PGA a new GC-method was used, based on reductive transformation of the relatively instable PGA into MA, which is stable enough for shipping and handling until final processing. The analysis of the post-exposure elimination shows that spot urine sampled in the morning after exposure and analysed for the sum of MA and PGA is the most reliable index for reflecing a preceding exposure to styrene.
D000328:Adult; D004781:Environmental Exposure; D006038:Glyoxylates / Q000652:urine*; D006801:Humans; D008297:Male; D008333:Mandelic Acids / Q000652:urine*; D008452:Maximum Allowable Concentration; D008722:Methods; D008991:Monitoring, Physiologic; D013343:Styrenes / Q000378:metabolism*
D006038:Glyoxylates; D008333:Mandelic Acids; D013343:Styrenes; C012482:phenylglyoxylic acid