title: Sulfated glycosaminoglycans of cells grown in culture: dermatan sulfate disappearance in successive fibroblast subcultures.
abstract: Sulfated glycosaminoglycan content of primary cultures of guinea pig embryo fibroblast in reported. It is shown that changes of sulfated glycosaminoglycans occur in successive subcultures: 1) disappearance of dermatan sulfate and 2) a slight increase in the proportion of heparitin sulfate. The sulfated glycosaminoglycan composition of the mammalian cell established lines: VERO, SIRK, Hep-2, MK-2, BGM, IB-RS-11 (S6) and 3T6 is also reported. It is shown that chondroitin sulfate AC and/or heparitin sulfate are the main glycosaminoglycans of the established cell lines (except 3T6). A possible role of these glycosaminoglycans in cell biology is discussed.
mesh_terms: D002460:Cell Line; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000378:metabolism; D003871:Dermatan Sulfate / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism
chemical_list: D002807:Chondroitin; D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006497:Heparan Sulfate