Division of the spleen cells and popliteal lymph nodes in non-continuous BSA gradient of the bovine serum albumin.
Lymphocytes from the popliteal lymph nodes and the spleen of a rabbit were divided by means of rotating in the gradient of bovine albumin (BSA). Nine fractions were obtained, and the percentage of the T lymphocytes as well as the total number of cells were determined.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D011817:Rabbits; D012710:Serum Albumin, Bovine; D013154:Spleen / Q000166:cytology
D012710:Serum Albumin, Bovine