Synthesis of combined protein antigens. Cross-linking of Hg-(p-chloromercuribenzoate) and--SH(thiolactone) substituted proteins.
The method of coupling of -Hg (p-chloromercuribenzoate) substituted proteins with protein substituted by -SH (thiolactone) groups was designed to obtain AnBm type copolymers not associated with An or Bm homopolymers. Reaction of p-chloromercuribenzoate with human serum albumin (HSA) was performed in 1.47 M imidazole. The conjugate was treated with thiolated proteins (human immunoglobulin G or human serum albumin). Disulfide linking of thiolated proteins was negligible under the conditions used. The total yield of polymerization reached the value 35%-75% of proteins taken for the reaction. It depended on the amount of reactive groups substituted and ratio of proteins A/B, used for cross-linking. The results were confirmed by theoretical calculations based on Bernoulli distribution.
D000941:Antigens; D055598:Chemical Phenomena; D002621:Chemistry; D002729:Chloromercuribenzoates; D006801:Humans; D007074:Immunoglobulin G; D009941:Organomercury Compounds; D011108:Polymers; D012709:Serum Albumin / Q000276:immunology; D013438:Sulfhydryl Compounds
D000941:Antigens; D002729:Chloromercuribenzoates; D007074:Immunoglobulin G; D009941:Organomercury Compounds; D011108:Polymers; D012709:Serum Albumin; D013438:Sulfhydryl Compounds