Dopamine-beta-hydroxylase-induced changes in the electrical conductance of bimolecular lipid membranes.
Dopamine-beta-hydroxylase (DBH), an enzyme that catalyzes the conversion of dopamine (DA) to norepinephrine (NE) in adrenal medullary chromaffin granules, increases the electrical conductance of bimolecular lipid membranes. The conductances increase requires both DA and Ca2+ and occurs in discrete steps. The conductance, which increases as the square of the DBH concentration, is nonselective for cations over anions and requires the native conformation of DBH. NE cannot replace DA.
D000311:Adrenal Glands / Q000201:enzymology; D000818:Animals; D002118:Calcium; D002417:Cattle; D002784:Cholesterol; D004298:Dopamine; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D004553:Electric Conductivity; D008051:Lipid Bilayers; D011199:Potentiometry
D008051:Lipid Bilayers; D002784:Cholesterol; D004299:Dopamine beta-Hydroxylase; D002118:Calcium; D004298:Dopamine