The development of DT-diaphorase in rat liver and its induction by benzo(a)pyrene.
The development of cytosolic DT-diaphorase--NAD(P)H dehydrogenase, quinone, EC 1.6.99.2--and its induction by benzo(a)pyrene has been studied in rat liver. DT-diaphorase belongs to the late suckling cluster, because the largest increase in activity can be observed 18 days after birth. A considerable activity is present, however, in the neonatal period. The activity of the enzyme can be prematurely induced by benzo(a)pyrene. A lag phase of 10 h can be observed before the activity of DT-diaphorase starts to increase. This increase in activity proved to be sensitive to inhibitors of mixed-function oxydase and RNA and DNA synthesis.
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001580:Benzopyrenes / Q000494:pharmacology*; D003600:Cytosol / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis; D004790:Enzyme Induction; D008099:Liver / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis*; D011808:Quinone Reductases / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis; D051381:Rats
D001580:Benzopyrenes; D012313:RNA; D004247:DNA; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases