title: Determination of clonazepam and its amino and acetamido metaoblites in human plasma by combined gas chromatography chemical ionization mass spectrometry and selected ion monitoring.
abstract: Our previously published gas chromatography chemical ionization mass spectrometry stable isotope dilution assay for clonazepam and its 7-amino metabolite in plasma has been modified to obtain more consistent recoveries of the amine metabolite and to permit the determination of the 7-acetamido metabolite of clonazepam. In addition, the manuscript describes the details of a method for a least squares analysis of the nonlinear standard curves associated with the use of certain stable isotope labeled analogs as internal standard.
mesh_terms: D000081:Acetamides; D000588:Amines; D001570:Benzodiazepinones / Q000097:blood*; D002849:Chromatography, Gas; D002998:Clonazepam / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D003201:Computers; D006801:Humans; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D000081:Acetamides; D000588:Amines; D001570:Benzodiazepinones; D007477:Ions; D002998:Clonazepam