Transcription of the non-repetitive genome in liver hypertrophy and the homology between nuclear RNA of normal and 12 H-regenerating liver.
The percentage of the non-repetitive genome transcribed and the complexity of nuclear RNA were estimated in normal and 12 h-regenerating rat liver. Nuclear RNA from normal or 12 h-regenerating liver hybridizes with approximately 6.1% of non-repetitive DNA (12.2% of the single-copy genome, assuming assymetric transcription). The estimated complexity of either of these nuclear RNA populations is 7.6 . 10(10) daltons, which is approximately 7 times higher than that calculated for polysomal mRNA. Cross hybridization experiments did not show differences between the nuclear RNA populations of normal and 12 h-regenerating liver. The results indicate that liver hypertropy (without hyperplasia) may be brought about without a large increase in the proportion of the non-repetitive genome transcribed.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D006984:Hypertrophy; D007700:Kinetics; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008115:Liver Regeneration; D008297:Male; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D012332:RNA, Heterogeneous Nuclear / Q000096:biosynthesis*; D051381:Rats; D014158:Transcription, Genetic
D012332:RNA, Heterogeneous Nuclear; D004247:DNA