Purification of the lectin from Datura stramonium.
The lectin from Datura stramonium can be inhibited by oligomers of N-acetylglucosamine. This property was exploited to purify the lectin by affinity chromatography on Sepharosefetuin. The purified lectin is a glycoprotein in having subunits of 40 000 and 45 000 mol.wt.
D000117:Acetylglucosamine; D002846:Chromatography, Affinity / Q000379:methods; D013286:Datura stramonium / Q000032:analysis*; D037102:Lectins / Q000302:isolation & purification*; D037121:Plant Lectins; D010946:Plants, Medicinal; D010947:Plants, Toxic
D037102:Lectins; D037121:Plant Lectins; D000117:Acetylglucosamine