title: Apparent trans control of murine beta-glucuronidase synthesis by a temporal genetic element.
abstract: A difference in the heat-inactivation kinetics between the beta-glucuronidases of C3HeB/FeJ and C57B1/6J mice was utilized to assess the mode of action of a temporal genetic element in controlling the expression of the beta-glucuronidase structural gene Gus. The heat-inactivation kinetics of liver and kidney beta-glucuronidase from F1 C3HeB/FeJ X C57B1/6J animals were intermediate with respect to the parental enzyme patterns, suggesting that equal concentrations of the two allelic products were present in beta-glucuronidase tetramers of F1 progeny. beta-glucuronidase heteropolymers assembled in vivo under conditions where equal concentrations of the two structural alleles of the enzyme were known to be present also exhibited intermediate heat-inactivation kinetics. These observations are consistent with a trans mode of action of a genetic element that controls the rate of murine beta-glucuronidase synthesis.
mesh_terms: D000483:Alleles; D000818:Animals; D005796:Genes; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D049671:History, 18th Century; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013045:Species Specificity
chemical_list: D005966:Glucuronidase