Biliary excretion of cyclohexylphenyl 4-[35S]sulphate in the guinea pig.
The metabolic fate and mode of excretion of cyclohexylphenyl 4-[35S]sulphate were studied in the guinea pig. Up to 54.8% of the dose appeared in the bile, the majority as unchanged ester. Substantial amounts of hydroxylated cyclohexylphenyl 4-[35S]sulphate were also excreted in the bile together with minor amounts of the corresponding glucuronic acid conjugate. When isolated guinea-pig livers were perfused with cyclohexylphenyl 4-[35S]sulphate the biliary components were the same as those in the intact animal, although the relative concentration of the hydroxylated derivative was significantly greater. When the hydroxylated derivative was re-injected into guinea pigs it was excreted almost entirely unchanged in the bile. However, in the rat, it was excreted in the bile as a glucuronic acid conjugate. These findings are discussed in relation to studies carried out in the rat [Hearse, Powell, Olavesen & Dodgson (1969) Biochem. Pharmacol. 18, 181--195] and to differences in enzyme activities in rat and guinea-pig liver. The results are also discussed in terms of the molecular-weight threshold for the excretion of anions in guinea-pig bile.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D003510:Cyclohexanes / Q000302:isolation & purification / Q000378:metabolism* / Q000652:urine; D005965:Glucuronates / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D051381:Rats; D013463:Sulfuric Acid Esters / Q000302:isolation & purification / Q000378:metabolism* / Q000652:urine; D013464:Sulfuric Acids / Q000378:metabolism*
D003510:Cyclohexanes; D005965:Glucuronates; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids