Effects of ageing, surface sialic acid and glycopeptides of erythrocytes on auto-rosettes in man.
Human red blood cells can bind in vitro to autologous peripheral blood lymphocytes forming auto-rosettes. The percentage of rosett-forming cells (A-RFC) depends on erythrocyte ageing in vivo. Old untreated cells given an A-RFC percentage lower as compared with the young ones. The same age groups of cells treated with neuraminidase show a parallel increase of A-RFC as compared with untreated cells. No significant difference is found between young and old cells with high concentrations of neuraminidase. The rosetting formation is inhibited by the pre-treatment of lymphocytes with erythrocyte glycopeptides released by trypsin. This suggests that auto-rosetting is mediated by erythrocyte surface glycopeptides in which sialic acid plays a role directly or not.
D000017:ABO Blood-Group System; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D006020:Glycopeptides / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D009439:Neuraminidase; D011955:Receptors, Drug / Q000276:immunology; D012397:Rosette Formation; D012794:Sialic Acids / Q000276:immunology
D000017:ABO Blood-Group System; D006020:Glycopeptides; D011955:Receptors, Drug; D012794:Sialic Acids; D009439:Neuraminidase