[Effect of hormones on the metabolism of the swine cell line IB-RS-2].
The biological actions of hydrocortisone and insulin on the growth of swine cell line IB-RS-2 were studied. The data obtained showed that hydrocortisone at 2, 4 and 8 x 10(-6) M inhibits cell growth. This effect is directly related to the hormone concentration and to the time of exposure of the cells to the hormone. This inhibitory effect was found to be related to the glucose added to the maintenance medium. On the other hand insulin at 50, 100 and 200 ng/ml increased cell growth and antagonized the inhibitory effect of hydrocortisone. However at 500 ng/ml insulin inhibited cell growth and it was citotoxic.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000378:metabolism*; D004347:Drug Interactions; D004357:Drug Synergism; D005947:Glucose / Q000378:metabolism*; D006131:Growth Inhibitors / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D007668:Kidney; D013552:Swine
D006131:Growth Inhibitors; D007328:Insulin; D005947:Glucose; D006854:Hydrocortisone
Influéncia de hormônios sobre o metabolismo de células da linhagem suína IB-RS-2.