title: On the role of thymus in hemopoietic differentiation.
abstract: This study was performed to investigate further the connection between the thymus and blood formation suggested by earlier findings. Bone marrow, after in vitro treatment with either anti-thy 1.2 (alpha Thy 1.2) or nonimmune serum and complement, was transplanted to two separate groups of lethally X-irradiated isogenic recipient mice of several different genotypes. Twelve studies were done, and macroscopic spleen-colony numbers were found to be similar in the two groups; however, differences were found when spleens were examined microscopically. The most striking and consistent finding was a decrease in granulopoietic colonies in recipients of alpha-Thy-1.2-treated marrow. There were less pronounced decreases in erythropoietic and in total colonies (all hemopoietic kinds). Ratios of erythropoietic to granulopoietic colonies were regularly increased. Anemia did not develop over a period of 4 months in chimeras given either kind of treated marrow.
mesh_terms: D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D005260:Female; D006400:Hematocrit; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011828:Radiation Chimera; D013950:Thymus Gland / Q000166:cytology*
chemical_list: D000961:Antilymphocyte Serum