Butanol extracts from myelin fragments-II. Some properties of 5-hydroxytryptamine binding.
The myelin fragments of rat brain stem were treated with butanol-water mixtures, and the butanol extracts (total extract=TE) were incubated with 5 x10(-7) M of C14.5-HT. After incubation, protein, lipid phosphorus and radioactivity were analyzed by Sephadex LH20 column chromatography. Two peaks of components eluted in chloroform-methanol 4:1 (peak I and II) showed the binding capacity for C14.5-HT. The displacement studies with unlabeled 5-HT (5 X 10(-4) M) suggested that peak II was the saturable binding component to 5-HT. On the other hand, butanol extracts from the synaptic membranes of rat brain stem did not show binding for C14.5-HT. Various compounds were studied to determine their inhibitory effects on the saturable binding of 5-HT to TE. The results indicated that acetylcholine, dopamine and tryptamine inhibited the 5-HT binding but LSD, reserpine, colchicine, vinblastine, 5-hydroxytryptophan (5-HTP) and 5-hydroxy-3-indole acetic acid (5-HIAA) had no effect.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000440:Butanols / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009186:Myelin Sheath / Q000378:metabolism* / Q000648:ultrastructure; D010743:Phospholipids / Q000032:analysis; D011485:Protein Binding / Q000187:drug effects; D011506:Proteins / Q000032:analysis; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism / Q000648:ultrastructure; D014020:Tissue Extracts / Q000378:metabolism*; D014363:Tryptamines / Q000494:pharmacology
D000440:Butanols; D010743:Phospholipids; D011506:Proteins; D014020:Tissue Extracts; D014363:Tryptamines; D012701:Serotonin; D000109:Acetylcholine; D004298:Dopamine