The amount of Zn, Fe, Cu, Mg and Ca in rat liver and their relation to cytosol proteins.
The amount of Zn, Fe, Cu, Mg and Ca in rat liver samples was analysed by atomic-absorption spectrophotometry and the association of Zn, Fe and Mg to cytosol proteins was studied by ultrafiltration. Nearly all of the Zn and Fe attached tightly to the protein molecules, while more than 30% of Mg was found to be "free" (ultrafilterable) in the cytosol.
D000818:Animals; D002118:Calcium / Q000032:analysis*; D003300:Copper / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D007501:Iron / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008274:Magnesium / Q000032:analysis*; D008297:Male; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D051381:Rats; D013054:Spectrophotometry, Atomic; D014462:Ultrafiltration; D015032:Zinc / Q000032:analysis*
D011506:Proteins; D003300:Copper; D007501:Iron; D008274:Magnesium; D015032:Zinc; D002118:Calcium