[Evaluation of the mutagenic activity of chemical substances in an in vitro human peripheral blood lymphocyte culture after their activation in the body of mammals].
To evaluate mutagenic activity of human metabolites a method is suggested for treating the culture of human lymphocytes with whole mammalian blood after their exposure to a chemical. This method was used to study the cytogenic activity of cyclophosphamide metabolites available in blood of CBA female mice after intraperitoneal injection of different doses of the chemical.
D000818:Animals; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000097:blood / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008677:Metaphase / Q000187:drug effects; D051379:Mice; D008808:Mice, Inbred CBA; D009153:Mutagens / Q000494:pharmacology*; D013997:Time Factors
D009153:Mutagens; D003520:Cyclophosphamide
Otsenka mutagennoĭ aktivnosti khimicheskikh veschestv v kul'ture limfotsitov perifericheskoĭ krovi cheloveka in vitro posle ikh aktivatsii v organizme mlekopitaiushchikh.