Alterations of rat intestinal disaccharide absorption by bile or conjugated bile acids.
Taurocholate showed different effects on the jejunal sucrose absorption, depending on the concentration of the bile acid: 3 mmol/l taurocholate inhibited to a small extent (-7%), 10 mmol/l taurocholate had no effect and 30 mmol/l taurocholate increased the sucrose absorption by 17%. The sucrose hydrolysis was augmented by 20% in the presence of 30 mmol/l taurocholate. 2 mmol/l taurochenodeoxycholate caused an increase of sucrose hydrolysis(+ 37%) as well as of sucrose absorption (+ 12%) in the jejunum. Pooled rat bile induced a 21% inhibition of the sucrose hydrolysis, while the sucrose absorption was unchanged. The jejunal maltose hydrolysis was unaffected by 3 mmol/l taurocholate, whereas 10 or 30 mmol/l caused a minimal increase (+ 3%). The absorption of the resulting glucose was reduced to 84--92%. In the ileum 10 mmol/l taurocholate did not change the sucrose hydrolysis and absorption, whereas 2 mmol/l taurochenodeoxycholate induced a 30% decrease. The investigated bile acids had no effect on the microscopic structure of the small intestinal mucosa. Hypertonic solutions caused an accumulation and exfoliation of PAS-positive material at the top of the villi, independent of the presence or absence of bile acids.
D000818:Animals; D001646:Bile; D001647:Bile Acids and Salts / Q000494:pharmacology*; D004187:Disaccharides / Q000378:metabolism*; D005856:Germ-Free Life; D007082:Ileum / Q000502:physiology; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000166:cytology / Q000502:physiology; D007421:Intestine, Small / Q000502:physiology*; D007583:Jejunum / Q000502:physiology; D008297:Male; D008320:Maltose / Q000378:metabolism; D051381:Rats; D013395:Sucrose / Q000378:metabolism; D013655:Taurochenodeoxycholic Acid / Q000494:pharmacology; D013656:Taurocholic Acid / Q000494:pharmacology
D001647:Bile Acids and Salts; D004187:Disaccharides; D013655:Taurochenodeoxycholic Acid; D013395:Sucrose; D013656:Taurocholic Acid; D008320:Maltose