title: Responses of mammalian metaphase chromosomes to endonuclease digestion.
abstract: Digestion of fixed metaphase chromosomes by endonucleases (micrococcal nuclease and DNase II) under optimal digestion conditions followed by Giemsa staining produces sharp banding patterns identical to G-bands. In 3H-thymidine labeled, synchronized metaphase cells of the chinese hamster (CHO line), the band induction is accompanied by the removal of DNA. The single strand specific nuclease S1 and DNase I do not produce such banding patterns.
mesh_terms: D000818:Animals; D001399:Azure Stains; D002415:Cats; D002417:Cattle; D002453:Cell Cycle; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure*; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism*; D006801:Humans; D008677:Metaphase; D012589:Sciuridae / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D001399:Azure Stains; D004247:DNA; D004720:Endonucleases