title: [Use of the transmission electron microscope in the analysis of wine contamination by asbestos].
abstract: A method for assessing chrysotile asbestos fibers in wines is described. Particles are isolated from wines samples by means of microfiltration through Nuclepore membrane. Retained particles are entrapped in a carbon layer which is observed under a transmission electron microscope fitted with an energy dispersive spectrometer. Chrysotile fibers identification is based on the determination of three characteristics- namely, morphology, elemental composition and crystal structure. Fibers are counted and measured (length and diameter) on an aliquot of the preparation area: numerical concentrations and their statistical significance are calculated. Several factors affect the accuracy and precision of this method. The feasibility for numerous laboratories to use this method is discussed.
mesh_terms: D001194:Asbestos / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D008722:Methods; D008851:Micropore Filters; D008854:Microscopy, Electron; D014920:Wine / Q000032:analysis*
chemical_list: D001194:Asbestos
vernacular_title: Utilisation du microscope électronique à transmission pour l'analyse de la contamination des vins par l'amiante chrysotile.