Cu2+-effects on mitochondrial resistance to sodium deoxycholate.
The effect of the preincubation of mitochondria with different doses of Cu2+ on the action of sodium deoxycholate (DOC) was studied. The amount of protein released from mitochondria upon their treatment with Cu2+ as an indice for the intactness of mitochondria was studied, too. On the account of Cu2+-influence on the level of mitochondrial SH groups Cu2+-effects on the action of DOC were compared with those of a thiol reagent p-chlormercuribenzoate (p-CMB). The results obtained show the following: Cu2+ in doses higher than 20 nmoles/mg protein induces conformational changes in mitochondrial membranes becoming more pronounced over 100 nmoles Cu2+/mg protein. These changes increase mitochondrial resistance to the action of DOC. Cu2+ in doses up to 100 nmoles/mg protein depending on the protein concentration of the suspension provokes a release of lower or higher amounts of protein from mitochondria. The higher resistance of mitochondria to DOC, as well as the release of protein from mitochondria after Cu2+-incubation are not due to mercaptide binding of SH groups as p-CMB does not affect these parameters.
D000818:Animals; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology*; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004347:Drug Interactions; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013696:Temperature
D002729:Chloromercuribenzoates; D011506:Proteins; D013438:Sulfhydryl Compounds; D003840:Deoxycholic Acid; D011189:Potassium Chloride; D003300:Copper