The influence of vinblastine and colchicine on renin secretion in vivo and in vitro.
The effect of the microtubule inhibitors colchicine and vinblastine on renin release in vivo and in vitro was studied. Injection of 0.5 mg/100 g i.v. of colchicine or vinblastine to furosemide treated rats on a low salt diet resulted in a decrease of plasma renin as well as plasma angiotensinogen concentration during a 5 h observation period. Renin release from rat kidney slices was diminished by vinblastine (5 x 10(-5) M), when basal or stimulated (by isobutylmethylxanthine and isoproterenol) renin release was measured. Colchicine at 5 x 10(-5) M had no effect under these conditions. Renin release from the isolated perfused rat kidney was increased 2--3 fold by vinblastine (10(-5) M) or colchicine (10(-4) M). The maximal response of renin release to isoproterenol (10(-7) M) was not changed when vinblastine (10(-5) M) or colchicine (10(-4) M) were present in the perfusion medium. The contrasting results cast considerable doubts on the suitability of microtubule inhibitors in studies on renin secretion.
D000808:Angiotensinogen / Q000097:blood; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology*; D004039:Diet, Sodium-Restricted; D066298:In Vitro Techniques; D007606:Juxtaglomerular Apparatus / Q000502:physiology*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008870:Microtubules / Q000502:physiology; D010477:Perfusion; D051381:Rats; D012083:Renin / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology*
D000808:Angiotensinogen; D012965:Sodium Chloride; D014747:Vinblastine; D012083:Renin; D003078:Colchicine; D002118:Calcium