title: A method of mass cultivation of Toxoplasma gondii in cell culture.
abstract: Large quantities of Toxoplasma gondii were cultivated in human larynx carcinoma cells (H.Ep 2, heteroploid). Virulent Toxoplasma gondii, strain RH, which were obtained from the peritoneal exsudate of mice 2 days after infection served as inoculum. As culture medium for the cells and for growth medium, Minimum Essential Medium (MEM) supplemented by 5% foetal calf serum and 200,000 IE/1 Penicillin plus 20 mg/1 Sulmycin was used. 2--3 days after inoculation, 150--200 times the amount of the inoculated Toxoplasmas could be harvested in one or several harvests.
mesh_terms: D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D006801:Humans; D007822:Laryngeal Neoplasms; D014122:Toxoplasma / Q000254:growth & development*
chemical_list: D003470:Culture Media