A gas chromatographic method for the determination of di- and polyamines in human urine.
A gas chromatographic method for the determination of di- and polyamines in human urine has been developed. The di- and polyamines have been extracted from standard solutions or from urine by a butanolic method modified to improve its sensitivity in relation to the low concentration of the amines in human normal urine. The quantitation is controlled by using two internal standards: 1,6-diaminohexane for Putrescine and Cadaverine, l-ephedrine for Spermidine and Spermine. Distilled water was the final solvent injected into the GLC column and the detector response was linear between 5 and 100 ng.
D002103:Cadaverine / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D008297:Male; D011073:Polyamines / Q000652:urine*; D011700:Putrescine / Q000652:urine; D013095:Spermidine / Q000652:urine; D013096:Spermine / Q000652:urine
D011073:Polyamines; D013096:Spermine; D002103:Cadaverine; D013095:Spermidine; D011700:Putrescine