title: Quality and rate of extracellular polysaccharides produced by Rhizobium meliloti and their inducing effect on polygalacturonase production in legume roots as derived from the presence of extrachromosomal DNA.
abstract: The ability of extracellular polysaccharides of different strains of Rhizobium meliloti to induce the production of polygalacturonase by roots of Medicago sativa seedlings has been studied. Some strains showed a high inducing activity while those derived from them, after treatment with acridine orange and in which extrachromosomal DNA was absent, did not show this character. A comparative study of polysaccharide production and preliminary studies on the chemical composition of the active fractions obtained after Sephadex G-25 filtration indicated that the monomers which form the active fractions are qualitatively and quantitatively different according to their origin.
mesh_terms: D000165:Acridine Orange / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D000455:Medicago sativa / Q000201:enzymology*; D010957:Plasmids / Q000187:drug effects; D011096:Polygalacturonase / Q000096:biosynthesis*; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000096:biosynthesis / Q000494:pharmacology*; D012231:Rhizobium / Q000187:drug effects / Q000378:metabolism*; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D011135:Polysaccharides, Bacterial; D006026:Glycoside Hydrolases; D011096:Polygalacturonase; D000165:Acridine Orange