Formation in vivo of deuterated methylthio metabolites of naphthalene from L-methionine (methyl-d--3).
Studies were carried out with deuterated methionine (methyl-d--3) to determine if methylthio metabolites of naphthalene were formed in vivo by reaction with methionine-derived metabolites. Rats were maintained on a methionine-free diet for nine days followed by eight days on the same diet supplemented with L-methionine-d--3 prior to administration of naphthalene. When naphthalene metabolites isolated from urine were characterized by GC and GC-MS procedures, it was found that the incorporation of deuterium into the methylthio metabolites, as well as into a catechol methyl ether, was approximately 40%. These results show that the methyl group was derived from methionine.
D000818:Animals; D002849:Chromatography, Gas; D004032:Diet; D008297:Male; D013058:Mass Spectrometry; D008715:Methionine / Q000172:deficiency / Q000378:metabolism*; D008968:Molecular Conformation; D009281:Naphthalenes / Q000378:metabolism*; D009355:Neomycin / Q000494:pharmacology; D051381:Rats
D009281:Naphthalenes; D008715:Methionine; D009355:Neomycin