[Short-time storage of human corneas in medium NCTC 135 (author's transl)].
20 human donor corneas were placed in a tissue culture medium (NCTC 135 with an additional content of 5% Actovegin resp. Solcoseryl) at +4 degrees C for 5 and 7 days. After that the temperature reversal effect was measured and the corneal endothelial cells were stained. The living cells were counted every 24 hours until no more cells could be seen. It could be shown, that compared with the MK-Medium 24% more living cells could be counted after 5 and 7 days storage in the medium. Possible causes of this improvement, practical consequences with including antibiotic prophylaxis are discussed.
D000183:Actihaemyl / Q000494:pharmacology; D003315:Cornea / Q000187:drug effects; D003470:Culture Media; D006801:Humans; D014015:Tissue Banks; D014021:Tissue Preservation / Q000379:methods*
D003470:Culture Media; D000183:Actihaemyl
Zur Kurzzeitlagerung menschlicher Hornhäute im Medium NCTC 135.