Toxicological studies of insecticides on Cullex quinquefasciatus Say and Aedes aegypti (L.).
Toxicity tests carried out on the larvae of A. aegypti and C. quinquefasciatus showed the former to be more tolerant of all insecticides tested, the order of toxicity being temephos greater than DDT greater than DDVP greater than malathion greater than lindane greater than carbaryl; also the second instar larvae of A. aegypti were more susceptible than fourth instar larvae. Enzyme kinetic studies on the total non-specific esterases and CarEs of adults and larvae of both species showed the Km values for total esterases of adult A. aegypti to be 0.333 mM vs 0.233 mM for C. quinquefasciatus; for adult CarEs it was 0.250 mM vs 0.220 mM. For total larval esterases of A. aegypti it was 0.112 mM vs 0.175 mM for C. quinquefasciatus: and for larval CarES it was 0.159 mM vs 0.213 mM respectively. Although some correlation between in vivo toxicity (LD50 values) and in vitro esterase inhibition (I50 values) between species could be discerned, overall correlation could not be established.
D000330:Aedes / Q000201:enzymology; D000818:Animals; D002219:Carbamates; D003465:Culex / Q000201:enzymology; D004950:Esterases / Q000378:metabolism; D005260:Female; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology; D007814:Larva / Q000201:enzymology; D007928:Lethal Dose 50; D008296:Malaysia; D009943:Organophosphorus Compounds
D002219:Carbamates; D007306:Insecticides; D009943:Organophosphorus Compounds; D004950:Esterases