[Emerson-Trinder reaction: study of various chromogens and analyses of the principle interferences (author's transl)].
Several phenolic compounds and aromatic amines were tested with a system consisting of hydrogen peroxide, peroxidase and 4-aminophenazone for their chromogenic activity in the Emerson-Trinder reaction: sulphonated 2,4-diclorophenol had the greatest activity, about 4 times that of unsubstituted phenol. The mechanism of action of serum components (serum proteins, bilirubin and ascorbic acid) which can interfere in the Emerson-Trinder reaction was also investigated by the same analytical system. Binding of phenolic compounds to serum was found to be responsible for protein interference: this can be overcome by increasing the phenolic compounds concentration. The interference by bilirubin and ascorbic acid is due to hydrogen peroxide consumption, in competition with the chromogenic substrate.
D000632:Aminopyrine; D001205:Ascorbic Acid; D001663:Bilirubin; D001786:Blood Glucose / Q000032:analysis*; D002784:Cholesterol / Q000097:blood*; D004003:Dichlorophen; D006801:Humans; D006861:Hydrogen Peroxide; D008722:Methods; D010544:Peroxidases; D010783:Photometry; D014527:Uric Acid / Q000097:blood*
D001786:Blood Glucose; D000632:Aminopyrine; D014527:Uric Acid; D002784:Cholesterol; D006861:Hydrogen Peroxide; D010544:Peroxidases; D001205:Ascorbic Acid; D001663:Bilirubin; D004003:Dichlorophen
La reazione di Emerson-Trinder: studio dei vari cromogeni e analisi delle principali interferenze.