Isolation and genetic characterization of a mutation affecting ribosomal resistance to cycloheximide in Tetrahymena.
A dominant mutation at a new locus affecting resistance to cycloheximide has been isolated by exploiting a synergistic relationship with a previously known mutation for cycloheximide resistance in Tetrahymena. The new mutation (ChxB) was induced in a line homozygous for ChxA and was recovered from that background by a new technique termed interrupted genomic exclusion. Segregation data from the interrupted genomic exclusion suggest that ChxA and ChxB are separate, linked loci showing 30% recombination. Minimal lethal doses of cycloheximide for the four possible combinations of the wild-type and mutant alleles of these two genes are: wild type 6 microgram/ml, ChxA 125 microgram/ml, ChxB 10 microgram/ml, ChxA-ChxB 175 microgram/ml.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005799:Genes, Dominant; D009154:Mutation; D010641:Phenotype; D014176:Protein Biosynthesis; D011995:Recombination, Genetic; D012270:Ribosomes / Q000187:drug effects; D013768:Tetrahymena / Q000235:genetics*
D003513:Cycloheximide