High pressure liquid chromatographic determination of patulin in apple juice.
Patulin was extracted from apple juice with ethyl acetate and the extract was purified by elution from a silica gel column with ethyl acetate-toluene. The eluate was concentrated, and patulin was determined by reverse phase high pressure liquid chromatography using a 25 cm Partisil-10 ODS column. The lower detection limit was 1 microgram/L and the mean recovery of patulin added to apple juice was 82.6 +/- 2.8 %. The patulin content ranged from less than 1 to 220 microgram/L for the 140 samples analyzed.
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000032:analysis*; D008832:Microchemistry; D009664:Norway; D010365:Patulin / Q000032:analysis*; D011714:Pyrans / Q000032:analysis*
D011714:Pyrans; D010365:Patulin