Characterisation of the antibodies detected by the film inhibition test for Mycoplasma bovis.
The class of antibody detected by the film inhibition test was determined. Density gradient ultracentrifugation and disulphide-bond reduction showed it to be 7S(IgG) immunoglobulin. Antibody absorption and ion exchange chromatography studies confirmed this and indicated that IgG2 is probably the major type of antibody involved.
D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D008722:Methods; D009174:Mycoplasma / Q000276:immunology*; D014461:Ultracentrifugation
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M