Pancreatic and hepatic balances of endogenous insulin and of glucose in the anaesthetized dog during primed and non-primed infusions of glucose and of mannitol.
Simultaneous monitoring of pancreatic and hepatic blood flows and serial analyses of blood glucose and immunoreactive insulin activity in arterial, portal, intestinal and hepatic venous blood were performed in anaesthetized laparotomized dogs. Based on these measurements, the rates of pancreatic insulin secretion, of hepatic insulin uptake and the hepatic glucose balance were computed. Under basal conditions pancreatic output was about 40% of the total hepatic insulin input. 30% of hepatic insulin inflow were actually taken up in the liver. During a primed i.v. glucose infusion (steady blood glucose concentration about 330 mg/100 ml) pancreatic and hepatic blood flows increase rapidly and the insulin secretion exhibits a biphasic pattern. The relative part of hepatic insulin inflow taken up in the liver remained unchanged. A non-primed i.v. glucose infusion provoked a slow increase of blood flow and a rapid but monophasic increase of insulin release. Under these conditions the relative hepatic insulin uptake was transiently increased. During both loads hepatic glucose balance switched from release to uptake. However the non-primed infusion was more effective. When pancreatic and hepatic blood flows were increased without any blood glucose alteration by an infusion of mannitol, insulin secretion did not change; hepatic uptake of insulin decreased, but hepatic glucose output was clearly reduced. A correlation between the rates of insulin secretion, of hepatic insulin inflow and of insulin uptake was observed under all conditions. However, the hepatic glucose balance was correlated to the inflow of insulin or glucose and to the hepatic insulin uptake under certain conditions in individual experiments only.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D004285:Dogs; D005260:Female; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008353:Mannitol / Q000378:metabolism*; D009929:Organ Size; D010179:Pancreas / Q000378:metabolism*
D001786:Blood Glucose; D007328:Insulin; D008353:Mannitol; D005947:Glucose