[Use of gas-liquid chromatography for the study of nitrilases and amidases (author's transl)].
A gas-liquid chromatographic procedure is described for the determination of nitrilasic and amidasic activities. This method allows to monitor the kinetics of the hydrolysis of volatile nitriles and amides and because of its sensitivity, to determine the Michaelis constants Km of the acetonitrilase and the acetamidase of Brevibacterium R 312. For these enzymes, a correlation is shown between the kinetics monitored by proton nuclear magnetic resonance spectroscopy and gas-liquid chromatography.
D000581:Amidohydrolases / Q000032:analysis*; D000619:Aminohydrolases / Q000032:analysis*; D001951:Brevibacterium / Q000201:enzymology; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D007700:Kinetics; D009570:Nitriles
D009570:Nitriles; D000581:Amidohydrolases; D000619:Aminohydrolases; C021867:nitrilase
Application de la chromatographie en phase gazeuse à l'étude des nitrilases et amidases.