Effect of actinomycin D or puromycin on microsomal testosterone hydroxylase activity enhanced by testosterone in female rat liver.
The injection of testosterone propionate for 4 successive days into female rats resulted in an increase of the in vitro conversion of the hydroxylated testosterones from testosterone by the hepatic microsomal fraction, but no change in the content of microsomal cytochrome P-450 occurred. Actinomycin D or puromycin, which was administered for 4 days with injections of testosterone propionate, prevented the enzyme induction.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D008862:Microsomes, Liver / Q000201:enzymology*; D011691:Puromycin / Q000494:pharmacology*; D051381:Rats; D013250:Steroid Hydroxylases / Q000096:biosynthesis*; D013739:Testosterone / Q000494:pharmacology*
D003609:Dactinomycin; D013739:Testosterone; D011691:Puromycin; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases