[Androgen activity of dysgenesic rats' testis. Histochemical, ultrastructural studies and radioimmunoassay of testosterone].
Androgen activity of rat testis submitted to a radiomimetic, Misulban, during prenatal development has been explored with different technics. Histochemical method shows the presence, in the interstitial cells, of delta5-3beta-hydroxysteroid dehydrogenase (delta5-3beta-HSD), and others enzymes of cellular metabolism, particularly the glucose-6-phosphate dehydrogenase (G6PDH) which is a glucidic enzyme implicated in the specific metabolism of steroidogenesis. From ultrastructural point of view interstitial cells contain the organels proper to steroidogenetic cells (important smooth endoplasmic reticulum, many voluminous mitochondria with tubular cristae). The plasma testosterone concentration measured by radioimmunoassay, confirms the androgenic function of interstitial tissue in dysgenesic testicles.
D000818:Animals; D002066:Busulfan / Q000494:pharmacology; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006651:Histocytochemistry; D007985:Leydig Cells / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D011375:Progesterone Reductase / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D013737:Testis / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D013739:Testosterone / Q000032:analysis* / Q000097:blood
D013739:Testosterone; D011375:Progesterone Reductase; D005954:Glucosephosphate Dehydrogenase; D002066:Busulfan
Activité androgène de testicules dysgénésiques de rats.