Analysis of mono- and disaccharides by high-performance liquid chromatography of the benzyloxime-perbenzoyl derivatives.
A group of biologically important mono- and disaccharides are separated by high-performance liquid chromatography of the benzyloxime-perbenzoyl derivatives on a normal-phase microparticulate column with a hexane-dioxane mixture as the eluting solvent. A single, quantifiable derivative of each sugar is formed easily. These derivatives are detected by UV absorption at either 230 or 254 nm, with a sensitivity in the picomole range at the former wavelength. The sugars in the residues from the evaporation of small aliquots of biologic fluids (10-100 microliter) are derivatized without prior isolation and are determined quantitatively by the use of appropriate internal standards. The analyses could be performed routinely with a simple, inexpensive instrument.
D001565:Benzoates / Q000032:analysis; D001593:Benzyl Compounds / Q000032:analysis; D001786:Blood Glucose / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000032:analysis* / Q000097:blood; D006801:Humans; D009005:Monosaccharides / Q000032:analysis* / Q000097:blood; D010091:Oximes / Q000032:analysis
D001565:Benzoates; D001593:Benzyl Compounds; D001786:Blood Glucose; D004187:Disaccharides; D009005:Monosaccharides; D010091:Oximes