[Effect of benzodiazepines on the fluorescence of tryptophan in human serum albumin, hemoglobin and erythrocyte ghosts].
The binding of chlorodiazepoxide, demethyl-chlorodiazepoxide, demoxepam, medazepam, diazepam and oxazepam was investigated on human serum albumin (HSA), ghosts and hemoglobin by quenching the fluorescence of tryptophan. The tryptophan fluorescence is quenched in a dose-dependent manner by these benzodiazepines with the exception of demoxepan, diazepam and oxazepam on hemoglobin. The calculated binding constants are in the range of 10(4)1 . M-1. It is concluded from the quenching of tryptophan-fluorescence that benzodiazepines are bound in a tryptophan containing area of the proteins.
D001569:Benzodiazepines; D004910:Erythrocyte Membrane / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure*; D006454:Hemoglobins; D006801:Humans; D007700:Kinetics; D008565:Membrane Proteins / Q000097:blood; D011485:Protein Binding; D011487:Protein Conformation; D012709:Serum Albumin; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D014364:Tryptophan
D006454:Hemoglobins; D008565:Membrane Proteins; D012709:Serum Albumin; D001569:Benzodiazepines; D014364:Tryptophan
Die Beeinflussung der Tryptophanfluoreszenz von Humanserumalbumin, Hämoglobin und Erythrozytenmembranen durch Benzodiazepine.