[Mapping of lengthy changes in the genomes of lambda-phages by using restriction endonucleases].
Fragmentation and mapping of DNA of two derivatives of lambda-phage, lambdapgal and lambda delta were done using restricting endonucleases R. EcoRI, R. BamHI, and R. SmaI. In DNA of lambdapgal phage a fragment of chromosome lambda of the wild type with molecular mass of 3.7--4.7 megadaltons to the left of the integration site was replaced with a fragment of chromosome of E. coli with the molecular mass of 2.3--3.3 MD containing a complete galactose operon. In the chromosome of lambda delta phage as compared with lambda-phage of the wild type there is a deletion of 5.2 MD in size. The localization of the deletion was determined. Possible ways of using DNA of lambdapgal in genetic engineering studies as the "pure" source of gal-operon for its transplantation using various restricting endonucleases are discussed.
D003090:Coliphages / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D005690:Galactose / Q000235:genetics; D005814:Genes, Viral; D006868:Hydrolysis; D008970:Molecular Weight; D009154:Mutation; D009876:Operon
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D005690:Galactose
Kartirovanie protiazhennykh izmeneniĭ genomov lambda-fagov s pomoshch'iu restriktiruiushchikh éndonukleaz.