Comparative methods of titration of Silverwater virus, an arthropod borne virus.
Silverwater virus (SIL) was titrated in vivo and in vitro. Using a microculture technique, TCID50 was found to be much more sensitive than both the plaque forming assay and the LD50. The latter was the least sensitive mode of titration of Silverwater virus. The addition of DEAE dextran and trypsin to the viral inoculum increased the number and diameter of the plaques.
D000818:Animals; D001103:Arboviruses / Q000302:isolation & purification*; D001681:Biological Assay; D002478:Cells, Cultured; D003637:DEAE-Dextran / Q000494:pharmacology; D007928:Lethal Dose 50; D051379:Mice; D014357:Trypsin / Q000378:metabolism; D010948:Viral Plaque Assay / Q000379:methods
D003637:DEAE-Dextran; D014357:Trypsin