title: Isolation of auxotrophs of Bacteroides fragilis.
abstract: Techniques employing ethane methanesulfonate and modified penicillin enrichment were developed whereby auxotrophs of Bacteroides fragilis could be readily isolated. Several auxotrophic phenotypes are described.
mesh_terms: D001441:Bacteroides fragilis / Q000235:genetics* / Q000378:metabolism; D005020:Ethyl Methanesulfonate; D005796:Genes; D009154:Mutation; D010400:Penicillin G
chemical_list: D005020:Ethyl Methanesulfonate; D010400:Penicillin G