Determination of molsidomine in plasma by high-performance liquid column chromatography.
A high-performance liquid chromatographic method is described for the analysis of the anti-anginal compound 5-ethoxycarbonyl-3-morpholinosydnonimine (Molsidomine) in human and dog plasma. The drug was extracted from plasma into chloroform and the analysis was carried out on a reversed-phase column, the column effluent being monitored by UV absorption at 312 nm. The method is sensitive (2 +/- 0.3 ng/ml) and specific. The method was applied to a study in which human volunteers received an aqueous solution of the drug and then, on a separate occasion, a tablet formulation. Peak plasma levels of 20--30 ng/ml (tablet) and 10--19 ng/ml (aqueous solution) were obtained following a 2-mg oral dose.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009025:Morpholines / Q000008:administration & dosage / Q000032:analysis / Q000097:blood*; D010069:Oxadiazoles / Q000097:blood*; D012996:Solutions / Q000032:analysis; D013558:Sydnones / Q000008:administration & dosage / Q000032:analysis / Q000097:blood*; D013607:Tablets
D009025:Morpholines; D010069:Oxadiazoles; D012996:Solutions; D013558:Sydnones; D013607:Tablets