Experimental gynogenesis in the newt species Pleurodeles waltlii and P. poireti.
Eggs of diploid females of Pleurodeles waltlii, inseminated by genetically inactivated sperm of Salamandra salamandra, have been treated by heat-shock or increased hydrostatic pressure during the first hour of their development. The resulting viable gynogenetic individuals show different degrees of ploidy although they mostly are diploid. The use of females with a pericentric inversion as a marker chromosome allow the chromosomal constitution to be clarified. Eggs of females heterozygous for a recessive semilethal mutation (ascite caudale, ac) subjected to the same experimental treatment gave 50% ac/ac embryos. Experiments with Pleurodeles poireti gave the same results as those with P. waltlii. These observations prove that gynogenetic Urodeles can be produced in large numbers. In their offspring the detection of inherent or spontaneous recessive mutations is greatly facilitated.
D000782:Aneuploidy; D000818:Animals; D002875:Chromosomes; D004625:Embryo, Nonmammalian / Q000648:ultrastructure; D005260:Female; D006358:Hot Temperature; D006874:Hydrostatic Pressure; D007621:Karyotyping; D012455:Salamandridae / Q000235:genetics*; D013045:Species Specificity; D014562:Caudata / Q000235:genetics*; D015053:Zygote / Q000648:ultrastructure