Growth characteristics of influenza virus type C in avian hosts. Brief report.
Influenza virus type C could be propagated to high yield in primary chick embryo kidney cell culture (PCEK) provided that trypsin (2 microgram/ml) was used as a medium supplement. The virus could also be titrated by plaque assay using PCEK host cells and influenza C virus that had been plaque-purified in PCEK cells could then be serially passaged to high titer using the allantoic route of 10--11-day-old embryonated eggs.
D002478:Cells, Cultured; D003470:Culture Media; D009975:Orthomyxoviridae / Q000254:growth & development*; D014357:Trypsin; D010948:Viral Plaque Assay; D014776:Virus Cultivation
D003470:Culture Media; D014357:Trypsin