title: Effect of papaverine on renin release in dogs in vivo and in vitro.
abstract: The effect of papaverine on renal function and renin release was investigated in dogs in vivo and in vitro. Intrarenal arterial infusion of 0.1 mg/kg/min of papaverine for 10 minutes caused a significant rise in renal blood flow, a significant decrease in renal vascular resistance, clearance and extraction ratio of creatinine and PAH and in the amount of filtered sodium, without altering arterial blood pressure. There was a significant increase in sodium excretion and in the excreted percentage of filtered sodium (TRFNa). Renin activity (PRA) of arterial blood and renal venous blood, veno-arterious PRA-difference and renin secretion increased significantly after papaverine infusion. In order to eliminate the effect of hemodynamic changes on renin secretion, the effect of papaverine (10(-5), 10(-4)M) was investigated in vitro in surviving canine kidney cortex slices. Papaverine caused a significant increase in renin release and in tissue cAMP concentration. This supports the assumption that the increase in renin secretion might be due to a direct effect on the juxtaglomerular apparatus, by blocking phosphodiesterase activity and by increasing the renal cAMP level.
mesh_terms: D000818:Animals; D003404:Creatinine / Q000378:metabolism; D004285:Dogs; D005260:Female; D006439:Hemodynamics / Q000187:drug effects; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000502:physiology; D008297:Male; D010208:Papaverine / Q000494:pharmacology*; D012083:Renin / Q000097:blood / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D014867:Water / Q000378:metabolism; D010130:p-Aminohippuric Acid / Q000378:metabolism
chemical_list: D014867:Water; D012964:Sodium; D003404:Creatinine; D010208:Papaverine; D012083:Renin; D010130:p-Aminohippuric Acid