Hyaluronidase release from guinea pig spermatozoa as affected by reproductive tract secretions and metabolic inhibitors.
Cauda epididymal sperm of mature guinea pigs were incubated (37 degrees, 5% CO2 in air). 10% of the total enzyme activity was released into the medium in 4 hr, 30% in 24 hr. Addition of lysolecithin resulted in rapid release of hyaluronidase. Vitamin C (0.54 mM), sodium fluoride (0.02 M), and cholesterol increased the rate of release whereas citrate (20 mM) diminished it. No effect upon hyaluronidase release was noted upon addition of KCN (10(-2)M), progesterone (250 microgram/ml), testosterone (500 microgram/ml), spermine (1.15 mg/ml), inositol (5.6 mM), or chloroquine phosphate (0.54 mM).
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002784:Cholesterol / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology; D005837:Genitalia, Male / Q000378:metabolism*; D006168:Guinea Pigs; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008297:Male; D013094:Spermatozoa / Q000201:enzymology*
D002951:Citrates; D002784:Cholesterol; D006821:Hyaluronoglucosaminidase; D001205:Ascorbic Acid