title: Some hemodynamic determinants of immune complex trapping by the kidney.
abstract: This study was undertaken to help clarify the relationship between capillary hemodynamic events and the tissue uptake of circulating immune complexes (IC). In each of 23 dogs, bovine serum albumin (BSA) and rabbit antiBSA soluble IC labeled with 125I were given by constant i.v. infusion, and IC uptake by a normally perfused kidney was compared to that of the contralateral kidney in which renal blood flow (RBF) was changed by renal artery constriction or raised ureteral pressure. In these same animals, IC uptake in 15 other major organ systems was also measured simultaneously. During IC infusion microspheres of 85Sr were injected to measure cardiac output and tissue blood flow, and red cells labeled with 51Cr were infused to mark tissue vascular volume. At completion of the IC infusion, tissue samples were taken from the kidneys and the 15 other major organs systems. From the isotope content of each tissue, we determined IC content, blood flow rate, vascular transit time, and fractional uptake of IC (FIC). In addition, glomeruli were isolated from renal cortex to assess IC uptake in glomerular versus renal nonglomerular tissue. We found that 1) for kidney, IC delivery rate, capillary hydrostatic pressure, and capillary ultrafiltration rate are less important than the plasma IC concentration in determining IC uptake; 2) for each organ studied, the principal determinant of IC uptake per gram of tissue, at any given PIC, is vascular volume per gram of tissue; 3) tissue vascular volume per gram of tissue may determine IC uptake per gram of tissue because tissue vascular volume determines the capillary surface area in contact with circulating IC or because tissue vascular volume determines tissue vascular transit time, at any given tissue blood flow rate.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D001794:Blood Pressure; D004285:Dogs; D006439:Hemodynamics; D007668:Kidney / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D007672:Kidney Cortex / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D007678:Kidney Glomerulus / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D008833:Microcirculation; D012039:Regional Blood Flow; D012306:Risk; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D000936:Antigen-Antibody Complex