title: Cold-sensitivity of a double mutant strain combining two ribosomal mutations in the ascomycete Podospora anserina.
abstract: A double mutant strain combining two ribosomal mutations conferring resistance to cycloheximide exhibits a cold-sensitive phenotype. At low temperature the biosynthesis of the 60S subunit is impaired. Genetic analysis of cold-resistant revertants have shown that this double mutant strain can be used efficiently to isolate new ribosomal mutations.
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D003080:Cold Temperature; D003513:Cycloheximide / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D009154:Mutation; D010641:Phenotype; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012270:Ribosomes
chemical_list: D012313:RNA; D003513:Cycloheximide