The spin method for recovering tissue larvae and its use in evaluating C57BL/6 mice as a model for the study of resistance to infection with Ascaris suum.
A centrifugation method for recovering tissue larvae is described and is proven to be superior to both Baerman and tissue digest methods for recovering larvae of Ascaris suum from the liver and lungs of infected C57BL/6 mice. Using the spin method for harvesting larvae, C57BL/6 mice were evaluated for their suitability in studies on specific host immunity to infection with A. suum.
D000818:Animals; D001196:Ascariasis / Q000276:immunology* / Q000469:parasitology; D001200:Ascaris / Q000302:isolation & purification*; D002498:Centrifugation; D004195:Disease Models, Animal; D007114:Immunization; D007814:Larva / Q000302:isolation & purification; D008099:Liver / Q000469:parasitology*; D008168:Lung / Q000469:parasitology*; D008722:Methods; D051379:Mice; D008810:Mice, Inbred C57BL