Instrumentation for direct observation of frozen hydrated specimens in the electron microscope.
Instrumentation and techniques are described for the transfer and observation of frozen hydrated specimens in the transmission electron microscope. The transfer is accomplished without the complexity of a vacuum transfer device but also without significant sublimation of specimen ice or frosting. Examples are given of the transfer and observation of thin sections of rapidly frozen muscle and of rapidly frozen thin film preparations of isolated cells.
D000818:Animals; D002417:Cattle; D005629:Frozen Sections; D008297:Male; D008854:Microscopy, Electron / Q000295:instrumentation*; D008867:Microtomy; D009132:Muscles / Q000648:ultrastructure*; D011817:Rabbits; D013094:Spermatozoa / Q000648:ultrastructure*