title: Purification of pancreatic lipase via its affinity for bile salts and apolar surfaces.
abstract: An improved method for the purification of pancreatic lipase in milligram amounts is described. Only one batch step and one chromatographic step are required to produce a lipase-colipase complex and a lipase largely free of colipase. Both species are lipid free; physical and kinetic data indicate that the predominant lipase species is lipase B.
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D003089:Colipases / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification* / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology*; D013657:Taurodeoxycholic Acid
chemical_list: D003089:Colipases; D005227:Fatty Acids; D013657:Taurodeoxycholic Acid; D008049:Lipase