[Change in the nonspecific cytotoxic activity of the lymphocytes depending on the method of their isolation].
The authors present the results of comparative study of nonspecific cytotoxicity of lymphocytes, obtained by different methods, on the cells of cultivated melanoma. The lymphocytes were obtained from defibrinated and heparinized blood of normal donors on a column made of cotton and of phycoll-verographine mixture. Lymphocytes obtained on the latter proved to possess greater cytotoxicity than those obtained on the former. Lymphocyte suspensions from the column, containing platelets were less toxic than lymphocyte suspensions without any platelets. Lymphocytes obtained on the column were more toxic against the melanoma cells of the 22nd--29th passage than against the cells of the 35th--41st passage. This regularity was more pronounced for lymphocyte-platelet suspensions.
D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D003602:Cytotoxicity, Immunologic; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008545:Melanoma / Q000276:immunology
Izmenenie nespetsificheskoĭ tsitotoksicheskoĭ aktivnosti limfotsitov v zavisimosti ot metoda ikh vydeleniia.