[Radiocompetitive estimation of 25-hydroxyvitamin D in human serum (author's transl)].
A radiocompetitive estimation of 25-hydroxyvitamin D in human serum is described. After extraction, a stage of purification by chromatography on a Sephadex LH 20 column permits separation of 25-hydroxyvitamin D from Vitamin D and other metabolites. The reaction of radiocompetition uses as tracer tritiated 25-hydroxycholecalciferol and as source of binding protein, rat renal cytosol. The characteristics of the binding protein and the presence in human plasma of significant quantities of 24,25-dihydroxyvitamin D render necessary the chromatographic stage. This technic permits one to measure simply and specifically 25-hydroxyvitamin D in the human serum, of which normal levels (average +/- standard deviation) are 12.88 +/- 5.42 microgram/l 3.2.10(-8) + 1.35.10(-8) mol/l).
D000328:Adult; D001667:Binding, Competitive; D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D006887:Hydroxycholecalciferols / Q000097:blood* / Q000302:isolation & purification; D008297:Male; D011863:Radioimmunoassay / Q000379:methods*
D006887:Hydroxycholecalciferols
Dosage radiocompétitif de la 25-hydroxyvitamine D dans le sérum humain.