Radioisotopic quantitation in microtitration plates by an autofluorographic method.
An autofluorographic record of drug titrations in microtitration plates has been obtained using cultures labelled with [35s] methionine after treatment with cytostatic drugs. By adding scintillation fluid directly to each culture well of the microtitration plate, and then centrifuging the evaporate the toluene and leave a flat even film of fluor, in situ scintillation is produced. When X-ray film is exposed to the plate the scintillation of individual wells is recorded as spots on the film. These may be interpreted by eye or by scanning densitometry.
D002460:Cell Line; D003720:Densitometry; D005470:Fluorometry / Q000379:methods*; D005472:Fluorouracil / Q000633:toxicity*; D005910:Glioma; D006801:Humans; D008715:Methionine; D014750:Vincristine / Q000633:toxicity*
D014750:Vincristine; D008715:Methionine; D005472:Fluorouracil