Stimulatory effect of adenosine on hepatic adenine nucleotide and energy charge levels in shocked rats.
In hemorrhagic shocked rats, the hepatic energy charge (ATP+1/2ADP)/(ATP+ADP+AMP) fell rapidly from 0.857 to 0.542 within 2 hours and was rapidly restored to normal levels after completing the reinfusion of shed blood. The total adenine nucleotide levels decreased slowly from 3.83 to 2.57 mumoles per g wet liver 2 hours after shock, and increased to normal levels 2 hours after reinfusion of shed blood. The intraperitoneal injection of 2 mg adenosine/100g body weight, 85 mg glucose/100 g body weight and 3.3 units insulin/100 g body weight, simultaneously with the reinfusion of shed blood, enhanced the recovery of energy charge and adenine nucleotide levels. The injection of 2 mg adenosine alone was ineffective in restoring the energy charge. However, administration of more than 6.7 mg adenosine/100 g body weight enhanced recovery of energy charge and adenine nucleotide levels, whether insulin was added or not.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D012769:Shock / Q000378:metabolism*; D012771:Shock, Hemorrhagic / Q000378:metabolism; D013997:Time Factors
D000227:Adenine Nucleotides; D007328:Insulin; D005947:Glucose; D000241:Adenosine