Can cell morphology in monolayer reveal its behaviour in aggregates?
The morphology of HeLa cells was altered to stellate shape by 0.2-1.0 mM butyric acid. At similar concentrations butyric acid did not alter the ability of HeLa cells to aggregate, but did inhibit the remodelling of the aggregates. Papaverine at 10 micrometers, on the other hand, had no effect on cells in monolayer, but severely inhibited the ability of cells to aggregate and the remodelling of the aggregates. Our results show that the effect of a drug on the morphology of HeLa cells in monolayer does not predict its effect in aggregates of cells which more faithfully approximate the behaviour of cells in tissue than the monolayer.
D002087:Butyrates / Q000494:pharmacology; D002449:Cell Aggregation / Q000187:drug effects; D006367:HeLa Cells / Q000166:cytology* / Q000187:drug effects; D010208:Papaverine / Q000494:pharmacology
D002087:Butyrates; D010208:Papaverine