Purification of Channel Catfish virus, a fish herpesvirus.
Channel Catfish virus was highly purified from host cells by precipitation with PEG-6000 and isopycnic centrifugation in a metrizamide gradient. As calculated from reconstruction experiments, only 0.09 and 0.05% respectively of the host DNA and the host proteins were recovered at the position of the viral band. The final recovery of infectivity was about 30%. Electron microscopy showed mostly intact viral particles.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient / Q000379:methods; D005399:Fishes; D006564:Herpesviridae / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D008793:Metrizamide
D008793:Metrizamide