Dissociation of fibronectin from gelatin-agarose by amino compounds.
Soluble fibronectin of human plasma was specifically dissociated at neutral pH from gelatin-agarose by several cationic amino compounds, notably the polyamines spermine, spermidine and putrescine, the basic amino acid arginine, and amino sugars. The neutral and acidic amino acids and the N-acetylated derivatives of amino sugars tested were ineffective. Gel-filtration experiments demonstrated that [14C]spermidine bound to fibronectin but not to gelatin.
D000596:Amino Acids; D000606:Amino Sugars; D005780:Gelatin; D006023:Glycoproteins / Q000097:blood; D011073:Polyamines; D011485:Protein Binding; D012685:Sepharose
D000596:Amino Acids; D000606:Amino Sugars; D006023:Glycoproteins; D011073:Polyamines; D005780:Gelatin; D012685:Sepharose