[Immunelectrophoretical evidence on inactivation of neuraminidase by limulus polyphemus-amebocyte-lysate (author's transl)].
Neuraminidase in concentrations up to 0.5 nu/0.1 ml (0.05 nu/0.1 ml) added to Limulus amebocyte lysate (LAL) in equal volumes (each 0.1 ml) releases a gelation reaction. Human AB-serum - subjected to immunelectrophoresis - is used as an indicator system to show that neuraminidase is bond to the LAL (incorporated in the gel). If there is a free amount of neuraminidase immunelectrophoresis of the serum glycoproteins indicates the typical changes previously described by Müller (6, 7). LAL does not cause any immunelectrophoretically detectable changes on serum glycoproteins by itself in the temperature range from +4 degrees C to 37 degrees C. It must be assumed that neuraminidase in a certain amount is releasing the coagulation system.
D004791:Enzyme Inhibitors; D006023:Glycoproteins / Q000378:metabolism; D008033:Limulus Test; D009439:Neuraminidase / Q000378:metabolism*
D004791:Enzyme Inhibitors; D006023:Glycoproteins; D009439:Neuraminidase
Immunelektrophoretischer Nachweis der Neuraminidase-Inaktivierung durch Limulus polyphemus-Amöbocyten-Lysat.