A correlative chemical and histochemical study of the O-acetylated sialic acids of human colonic epithelial glycoproteins in formalin fixed paraffin embedded tissues.
Procedures are described for the isolation and identification of epithelial glycoproteins from formalin fixed, paraffin embedded specimens of human large intestine. The side chain O-acetylation patterns of the sialic acids of these glycoproteins were surprisingly similar to those of purified glycoproteins prepared from epithelial cells obtained from the same tissue before fixation. These results were consistent with those obtained by histochemical procedures performed on representative sections taken from the same tissue blocks. The methodology described permits a direct correlation of chemical and histochemical results obtained from the study of colonic epithelial glycoproteins of both normal and diseased tissues. It eliminates some of the difficulties associated with interpretation of the results by either discipline and may provide new information which would be unavailable by either chemistry or histochemistry alone.
D000107:Acetylation; D000818:Animals; D003106:Colon / Q000032:analysis*; D004848:Epithelium / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D051381:Rats; D012794:Sialic Acids / Q000032:analysis*
D006023:Glycoproteins; D012794:Sialic Acids