Down regulation of insulin receptors in primary cultures of adult rat hepatocytes in monolayer.
Down regulation of insulin receptor number by insulin at physiological concentrating (10(-9) M) has been demonstrated in primary cultures of adult rat liver parenchymal cells in monolayer. However, because insulin is rapidly degraded in this culture system, special efforts are necessary to maintain the concentration of insulin in the medium in order to evoke the down regulation phenomenon.
D000818:Animals; D002478:Cells, Cultured; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D011972:Receptor, Insulin / Q000187:drug effects / Q000378:metabolism*
D007328:Insulin; D011972:Receptor, Insulin