[Respiratory system of Pichia guilliermondii yeasts with different levels of flavinogenesis].
The yeast Pichia guilliermondii was grown on media with different content of iron and its respiration system was studied. When the yeast was cultivated on a complete medium, its respiratory chain operated at the maximum rate in the exponential growth phase, i. e. all the three points of phosphorylation were involved; cytochrome oxidase was the only terminal oxidase. When the growth was decelerated and at the stationary phase, the alternative autooxidable cyanide-resistant pathway inhibited with salicyl hydroxamate partly functioned. Iron deficiency in the medium resulted in a two-three-fold decrease in the content of total and non-hemin iron in the cells, changes in the character and rate of growth, a decrease in the biomass yield, a high rate of flavinogenesis, a slight decrease in the respiration activity, though no drastic changes in the respiration system occurred. This system is represented, as in the case of cells grown on a complete medium, by a typical cytochrome system and an alternative autooxidable pathway. The absence of principal differences in the respiration systems of normal and iron-deficient cells, as well as the operation of the first point of coupling in flavinogenic cells, makes it doubtful that Fenh-proteins of the first segment of the respiratory chain are involved in the regulation of flavinogenesis.
D001203:Ascomycota / Q000378:metabolism*; D003580:Cytochromes / Q000032:analysis; D005415:Flavins / Q000096:biosynthesis*; D000090463:Iron Deficiencies; D010101:Oxygen Consumption; D010843:Pichia / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D011190:Potassium Cyanide / Q000494:pharmacology; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism
D003580:Cytochromes; D005415:Flavins; D011190:Potassium Cyanide
Dykhatel'naia sistema drozhzheĭ Pichia guilliermondii pri razlichnykh urovniakh flavinogeneza.