Adaptation of the ammoniacal silver reaction to cytochemical demonstration of myelin basic protein.
A modification of Black and Ansley's ammoniacal silver reaction (ASR) for histones is proposed for visualizing myelin basic protien (MBP) in the nervous system. The reaction is performed on histological sections of tissues fixed in neutralized formalin-alcohol and delipidized in the course of the routine paraffin embedding. The deparaffinized sections are again treated with formalin in order to make the "unmasked" by the delipidization basic groups of MBP reactive to ammoniacal silver. After treatment with this reagent MBP of the myelin sheaths of the nerve fibres is impregnated brownish-black. Deparaffinized sections subjected to an extraction of MBP with hydrochloric acid exhibit a negative reaction at the level of the myelin sheaths the same reaction being preserved at the level of the nuclear histones. The reaction is positive in paper spots of nervous tissue extracts obtained with the same acid. These assays indicate the specificity of the modified ASR. The method can be used for studies on the processes of myelination and demylination in normal histogenesis and in pathology of the nervous tissue.
D000818:Animals; D002417:Cattle; D002531:Cerebellum / Q000032:analysis*; D006168:Guinea Pigs; D006651:Histocytochemistry / Q000379:methods*; D006657:Histones / Q000032:analysis; D009185:Myelin Proteins / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis; D011817:Rabbits; D012834:Silver; D013116:Spinal Cord / Q000032:analysis*
D006657:Histones; D009185:Myelin Proteins; D009419:Nerve Tissue Proteins; D012834:Silver