Limitations of Culling PATS/KOH/PAS method of differential diagnosis of metastatic colorectal cancer.
Investigations of Culling et al. showed the possibility of using KOH/PAS effect (visualisation of C7 and C8O-acylated sialic acids characteristic to colon mucin) for differential diagnosis of metastatic colorectal cancer. Our investigations revealed, however, KOH/PAS positive mucin prevailing in some cases of gallbladder, pancreatic and gastric cancer. This makes serious limitation of Culling method in the routine diagnostic work.
D001984:Bronchial Neoplasms / Q000175:diagnosis; D003110:Colonic Neoplasms / Q000175:diagnosis*; D003937:Diagnosis, Differential; D005260:Female; D005706:Gallbladder Neoplasms / Q000175:diagnosis; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D009077:Mucins / Q000032:analysis*; D009362:Neoplasm Metastasis; D010190:Pancreatic Neoplasms / Q000175:diagnosis; D012004:Rectal Neoplasms / Q000175:diagnosis*; D013274:Stomach Neoplasms / Q000175:diagnosis; D014594:Uterine Neoplasms / Q000175:diagnosis
D009077:Mucins