Elemental analysis of frog outer segment and fuscin granule by means of x-ray microanalyzer.
Elemental analysis was made on the rod outer segments and fuscin granules in the frog eye by means of an energy-dispersive X-ray spectrometer combined with an electron microscope. The materials were prepared from the unfixed fresh retina by means of air-dried or freeze-dried cryosection, freeze-substitution, or the freeze-drying embedding method. An outer segment in the air-dried cryosection showed P, K, S, Cl, and Ca peaks. The dry section of freeze-substituted or freeze-dried embedded outer segment showed P, K, S, and Cl; K was lost in the wet section. The fuscin granules showed prominent peaks of Ca and Zn constantly, in addition to S, Cl, Mg, K, and occasionally Cu. The K peak was also lost in the wet section.
D000818:Animals; D001001:Anura; D001578:Benzopyrans / Q000032:analysis*; D002118:Calcium / Q000032:analysis; D003300:Copper / Q000032:analysis; D004577:Electron Probe Microanalysis; D010786:Photoreceptor Cells / Q000032:analysis* / Q000648:ultrastructure; D010857:Pigment Epithelium of Eye / Q000032:analysis* / Q000648:ultrastructure; D011188:Potassium / Q000032:analysis; D011898:Ranidae
D001578:Benzopyrans; D003300:Copper; D011188:Potassium; D002118:Calcium