Radioimmunological quantitation of human group-II pepsinogens.
A solid-phase sandwich radioimmunoassay was developed to quantitate human group-II pepsinogens in plasma. The test detected pepsinogen II in a concentration range of 0.25--64.0 ng/ml using sample volumes of 125 microliter. Purified group I pepsinogens showed no response up to concentrations of 100 microgram/ml. In apparently healthy donors, we observed mean plasma concentrations of 20.3 ng/ml (males) and of 15.5 ng/ml (females).
D000906:Antibodies; D001666:Binding Sites, Antibody; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology; D006801:Humans; D008297:Male; D010435:Pepsinogens / Q000097:blood* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D012737:Sex Factors
D000906:Antibodies; D010435:Pepsinogens