[RNA transport from rat liver cell nuclei in vitro. Release of rapidly labeled RNA from isolated nuclei in a cell-free system in the presence of RNAse affectors].
A cell-free system was developed and characterized, which supported RNA release from isolated rat liver cell nuclei. The RNA release in the system seemed to be dependent on the presence of ATP as an evergy source and of dialized cytosol as far as on the temperature level in the incubation mixture. In vitro effects of a number of RNase affectors from cytoplasm and of related exogenous compounds on the RNA release were studied. It was shown that RNase inhibitors such as heparin, PVS and rat liver cytoplasmic RNA were capable to stimulate the RNA release, while the natural inhibitor from liver cytosol failed to influence the RNA relase. Spermidine and PCMB lowered the rate of RNA release from nuclei. The results are discussed in connection with possible role of nuclear RNases and cytoplasmic factors in nucleocytoplasmic transport of RNA.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism*; D002474:Cell-Free System; D003600:Cytosol / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D012313:RNA / Q000378:metabolism*; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism*
D012313:RNA; D000255:Adenosine Triphosphate; D012260:Ribonucleases
Transport RNK iz iader kletok pecheni in vitro. Vykhod bystrometiashcheĭsia RNK iz izolirovannykh iader v beskletochnoĭ sisteme v prisutstvii effektorov RNKaz.