The effect of age on amino acid composition of human skin collagen.
To study the effect of age on amino acid composition of human skin collagen, skins from 8 women aged 0 to 93 were extracted with acetone and EDTA and then gelatinized. Gelatin was purified by extraction with chloroform-methanol and DEAE cellulose chromatography. Hydroxyproline was spectrophotometrically determined and its contents were essentially the same (12.6%). Amino acid analysis indicated no significant age-related variations in the contents of proline, hydroxyproline, lysine, and hydroxylysine over the range of 0 to 93 years of age. The content of cysteine was reasonably low (0.4 to 0.6 per 1,000 amino acid residues) except in the cases of those 0 and 93 years of age. Aldehyde content was slightly lower in young cases than in infant and old cases. These results indicate that the changes in cross-links derived from aldehyde may be responsible for the effect of age.
D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging; D000447:Aldehydes / Q000032:analysis; D000596:Amino Acids / Q000032:analysis*; D002648:Child; D003094:Collagen / Q000032:analysis*; D003545:Cysteine / Q000032:analysis; D005260:Female; D006801:Humans; D006901:Hydroxylysine / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D007231:Infant, Newborn; D008239:Lysine / Q000032:analysis; D008875:Middle Aged; D011392:Proline / Q000032:analysis; D012867:Skin / Q000032:analysis*
D000447:Aldehydes; D000596:Amino Acids; D006901:Hydroxylysine; D003094:Collagen; D011392:Proline; D008239:Lysine; D003545:Cysteine; D006909:Hydroxyproline