Studies on the influence of different drugs upon the proliferative answer of ASMC and fibroblasts in culture depending on drug concentration and exposition time.
We investigated the proliferation of fibroblasts and A.S.M.C. in culture as a function of concentration and exposition time of D-Penicillamine, Prednisolone, Acetylsalicylic Acid and Chloroquine. For every drug we found a concentration that caused only small initial inhibition of cell growth but did not alter the kind of further proliferation compared with controls. This "optimal inhibiting drug concentration" was characteristic of the drug but uncharacteristic of the cell type. Lower concentrations lead to a slight increase of proliferation, higher concentrations to a decrease of proliferation and finally after specific times of exposure to cell degeneration. Chloroquine showed no proliferation increasing effect even in doses much lower than the equivalent human daily drug dose. The equivalent human daily drug dose for Chloroquine was much higher than the optimal inhibiting drug concentration whereas that for the other drugs was the same as the optimal inhibiting drug concentration. For each drug there was a linear correlation between the degree of inhibition of cell proliferation and the logarithm of drug concentration on every occasion the cell growth was examined. All the relationships demonstrated were true of both fibroblasts--and A.S.M.C.--cultures, which indicates that there is no connection between the type of cell and its reaction to these, specific, chemical, drugs. Our results show that culture methods are suitable for the study of cell drug interactions.
D000818:Animals; D001011:Aorta / Q000187:drug effects*; D001241:Aspirin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002738:Chloroquine / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008168:Lung / Q000196:embryology; D009130:Muscle, Smooth / Q000187:drug effects; D010396:Penicillamine / Q000494:pharmacology; D011239:Prednisolone / Q000494:pharmacology; D013552:Swine; D013997:Time Factors
D002738:Chloroquine; D011239:Prednisolone; D010396:Penicillamine; D001241:Aspirin