Effects of phenobarbital administration on levels of physiological antioxidants in rat liver.
Administration of phenobarbital to rats over a period of 5 days was shown to increase hepatic lipid peroxidation concomitant with induction of cytochrome P-450 and cytochrome c reductase. The purpose of this study was to determine whether the increase in lipid peroxidation was due to a lowering in hepatic antioxidants. The results show that increased lipid peroxidation was not due to a decreased level of antioxidants since the fat-soluble antioxidants were unchanged and ascorbate, a water-soluble antioxidant, was elevated. The relationship of the increase in hepatic ascorbate to enhanced lipid peroxidation is discussed.
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D003579:Cytochrome Reductases / Q000096:biosynthesis; D004353:Drug Evaluation, Preclinical; D004790:Enzyme Induction / Q000187:drug effects; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D010084:Oxidation-Reduction; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats
D000975:Antioxidants; D003577:Cytochrome P-450 Enzyme System; D003579:Cytochrome Reductases; D001205:Ascorbic Acid; D010634:Phenobarbital