title: Genetic variation at a locus (TAM-1) for submaxillary gland protease in the mouse and its location on chromosome No. 7.
abstract: Electrophoretic and activity variants for a testosterone-induced esteroprotease have been discovered in submaxillary glands from inbred strains of mice. The enzyme is tentatively designated tamase [TAM-1] and the variant genetic locus is Tam-1. The alleles Tam-1a and Tam-1b determine electrophoretically distinct zones of tamase activity, while Tam-lc produces no detectable enzyme activity. Data from recombinant inbred strains and B6AF1 X B6 and B6D2F1 X B6 backcrosses established linkage of Tam-1 to glucose phosphate isomerase (Gpi-1), pink-eyed dilution [p] and beta-hemoglobin (Hbb) on chromosome 7. The gene order is Gpi-1--Tam-1--p--HBB. Analysis of congenic resistant strains indicates that Tam-1 is closely linked to the minor histocompatibility locus, H-4. TAM-1 was not cross-reactive with antisera to mouse nerve growth factor, submaxillary renin, or tamases A and D.
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D005796:Genes; D014644:Genetic Variation; D049671:History, 18th Century; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010447:Peptide Hydrolases / Q000235:genetics*; D013363:Submandibular Gland / Q000201:enzymology*
chemical_list: D010447:Peptide Hydrolases