[Autoradiographic detection of tritiated choline chloride in brain tissue. A method of microscopic detection in paraffin sections].
It is presented a autoradiographic method for microscopical detection of 3H-choline chloride activity after intraventricular application in freeze-dried and paraffin-embedded brain sections. The 10 micrometer thick sections are treated with diethyl ether and than exposed during 90 days above calcium chloride at 4 degree C using the sandwich technique (with stripping film). After separate film explanation and section staining the film and the brain sections are mounted together.
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001923:Brain Chemistry; D002712:Chlorides / Q000032:analysis; D002794:Choline / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods*; D007276:Injections, Intraventricular; D008297:Male; D051381:Rats
D002712:Chlorides; D002794:Choline
Autoradiographischer Nachweis von tritiiertem Cholinchlorid im Hirngewebe. Eine Methode zum lichtoptischen Nachweis im Paraffinschnitt.