Induction of differentiation in mouse neuroblastoma cells.
In the presence of 1--2% dimethyl sulfoxide (DMSO), mouse neuroblastoma cells are induced to differentiate morphologically as well as electrically. In addition, treatment of neurolbastoma cells with 2% DMSO results in a marked increase in the veratridine-activated K+ or Rb+ efflux. At 4% DMSO, neurite outgrowth is completely repressed and electrical activity is poorly developed. However, at this concentration, the cells have a relatively high resting potential which suggested that membrane components determining passive and active permeability properties are not necessarily under coordinated control. Induction of differentiation by 2% DMSO is also accompanied by an increase in a heavier molecular form of acetylcholinesterase sedimenting at 10.5S. The effect of other agents on the growth and differentiation of neuroblastoma cells is also presented.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology; D009447:Neuroblastoma / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D012964:Sodium / Q000378:metabolism
D007473:Ion Channels; D012964:Sodium; D000110:Acetylcholinesterase; D004121:Dimethyl Sulfoxide