title: A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood.
abstract: An improved and short method is described for the isolation of intact eosinophilic leukocytes from horse blood with high yield (1--1.5 g/20 l). Viability and purity of the preparations were verified by light and electron microscopy and by the trypan blue exclusion test. Isolated eosinophils were 98--100% pure, intact and viable, and they could be shown to phagocytise immune-complexes.
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods; D002498:Centrifugation; D004804:Eosinophils / Q000166:cytology*; D006736:Horses