Storage of macrophages at -196 degrees C.
Oil induced mice peritoneal cells were stored at -196 degrees C for 3 months, using dimethyl sulfoxide (DMSO) as a cryoprotective medium. Recovery counts upon thawing were about 90%. Macrophages retained their phagocytic activity of SRBC in 89% and there were no significant differences in spontaneous migration, expression of membrane immune receptors and non-specific cytotoxicity of frozen-thawed cells compared with the controls.
D000818:Animals; D001665:Binding Sites; D002464:Cell Migration Inhibition; D003176:Complement C3; D003451:Cryoprotective Agents; D003602:Cytotoxicity, Immunologic; D005260:Female; D005615:Freezing; D007141:Immunoglobulin Fc Fragments; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D010587:Phagocytosis; D011309:Preservation, Biological / Q000379:methods*
D003176:Complement C3; D003451:Cryoprotective Agents; D007141:Immunoglobulin Fc Fragments