On the proper use of the Soret band for hemoglobin detection in erythrocytic cells.
Intracellular hemoglobin detection by light microscopy in the Soret band (414nm) is a sensitive means of correlating morphology and biochemical function in studies of erythrpoiesis. Correct application of the technique requires a light source with strong emission in the near ultra-violet, a filter with a pass-band at 414nm, a preparation of living cells or an unfixed smear, and a receiver that is sensitive to the Soret wavelength. The human eye is very insensitive to light at 414nm and quite sensitive to stray green light: it is consequently much inferior to a black and white television or camera film for viewing a Soret image. The appearance of hemoglobin during maturation of erythroid cells in human bone marrow can be detected with greater sensitivity by this method than by a peroxidase-benzidine stain and it is the only method applicable to living cells.
D000818:Animals; D001854:Bone Marrow Cells; D004912:Erythrocytes / Q000032:analysis*; D004920:Erythropoiesis; D006454:Hemoglobins; D006801:Humans; D008027:Light; D051381:Rats
D006454:Hemoglobins