Metabolite levels, redox states, and gluconeogenic enzyme activities in livers of rats fed diets containing 1,3-butanediol.
Fat-free diets containing 1,3-butanediol (BD) were fed to rats. The concentration of metabolites in quick-frozen liver and the activities of kidney and liver gluconeogenic enzymes were examined. The free pyridine and adenine nucleotide ratios were calculated from measured intermediary metabolites. The concentrations of lactate, pyruvate, alpha-oxoglutarate, and glucose were significantly decreased in rats fed BD, while the acetoacetate and beta-hydroxybutyrate concentrations were increased in the BD-fed rats. The ratios of the free cytoplasmic [NAD+]/[NADH] and [NADP+]/[NADPH] were significantly decreased. Phosphoenolpyruvate carboxykinase activity was significantly increased in both kidney and liver of rats fed BD. These changes in metabolite levels and enzyme activities paralleled the effects seen in mild starvation, and were similar to reported changes observed when dietary fat was present.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002072:Butylene Glycols / Q000494:pharmacology*; D004032:Diet; D004435:Eating / Q000187:drug effects; D005943:Gluconeogenesis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D010085:Oxidative Phosphorylation / Q000187:drug effects; D051381:Rats
D000227:Adenine Nucleotides; D002072:Butylene Glycols