Enzymatic synthesis of H-active pentaglycosylceramide by human bone marrow fucosyltransferase.
Synthesis of two fucoglycolipides I and II was obtained from GDP-fucose and lacto-N-neotetraosylceramide using either solubilized or particulate enzymic preparation from human bone marrow. Fucoglycolipid I on the basis of its chromatographic mobility and immunoprecipitation with Ulex europeus lectin was identified as H blood group active penta glycosylceramide. Fucoglycolipid II did not react with Ulex europeus and its fucosyl residue was hydrolyzed by alpha (1 leads to 3/4)-L-fucosidase of Trichomonas fetus. Most probably the fucose residue of glycolipid II was linked to N-acetyloglucosamine by 1 leads to 3 linkage.
D000017:ABO Blood-Group System; D001853:Bone Marrow / Q000201:enzymology / Q000378:metabolism*; D005647:Fucosyltransferases / Q000378:metabolism*; D005915:Globosides / Q000378:metabolism; D006028:Glycosphingolipids / Q000096:biosynthesis* / Q000097:blood / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D012995:Solubility; D013379:Substrate Specificity
D000017:ABO Blood-Group System; D005915:Globosides; D006028:Glycosphingolipids; D005647:Fucosyltransferases; D006602:Hexosyltransferases