[A 3H-thymidine paper strip method for stimulation of lymphocytes. I. Culture conditions for human or murine lymphocytes and reproducibility of the paper strip method].
A simple method for stimulation of human or murine lymphocytes is described. As a possible more rapid test than the standard estimation of [3H]-thymidine uptake after solubilisation of cell sediments, paper strip counting in a liquid scintillation counter was studied in 80 individuals and 20 mice. Counting efficiency approaches 1%, the reproducibility is good within the acceptable 16% range.
D000818:Animals; D001692:Biological Transport; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008722:Methods; D051379:Mice; D010209:Paper; D013045:Species Specificity; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D013936:Thymidine
Die 3H-thymidin-Plättchen-Modifikation des Lymphozytentransformationstestes. I. Kultivierungsbedingungen für Menschen-sowie Müse-Lymphozyten und Reproduzierbarkeit der Papier-Plättchen-Technik.