[Comparative study of the activities of dehydrogenases and diaphorases. Basis of the technic].
In order to reveal dehydrogenase and diaphorase in spinal ganglia neurons of 12-day-old chick embryos in cryostat sections, the following modifications of the medium were used: for dehydrogenase - sodium salt substrate 50 mM, NAD or NADPh 0.75 mM, nitro-BT 0.61 mM, phosphate buffer pH 7.2 15 mM, NaCl 50 mM, MgCL2 5 mM, for diaphorase - NAD-N2 or NADHh-N2 0.78-0.66 mM, NaCl 100 mM. To compare relative activity of the enzymes (optic density of histochemical preparations determined cytophotometrically) it is suggested to calculate the values obtained during proportional development of the staining regarding the time unit (hour). The possibility to compare the data obtained with the results of biochemical investigations is discussed, as well as an attempt is made to represent graphically metabolic peculiarities of various cell types.
D000818:Animals; D002642:Chick Embryo; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000201:enzymology; D006651:Histocytochemistry / Q000379:methods; D010088:Oxidoreductases / Q000378:metabolism*
D010088:Oxidoreductases; D008058:Dihydrolipoamide Dehydrogenase
Sravnitel'noe izuchenie aktivnosti degidrogenaz i diaforaz; obosnovanie metoda.