title: Liquid culture of Diplodia maydis.
abstract: Diplodia maydis, a Zea mays L. stalk rot causing fungus, was grown in Czapek-Dox broth and modifications of Fries liquid media using combinations of 1% cellulose, 1% sucrose and ammonium or nitrate-nitrogen. Conditions are defined to yield consistently maximum mycelial dry weight in two days in contrast to the usually reported five-day incubation period.
mesh_terms: D002482:Cellulose; D003470:Culture Media; D005947:Glucose; D003904:Mitosporic Fungi / Q000254:growth & development*; D009566:Nitrates; D010935:Plant Diseases; D000644:Quaternary Ammonium Compounds; D013395:Sucrose; D003313:Zea mays / Q000382:microbiology
chemical_list: D003470:Culture Media; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D013395:Sucrose; D002482:Cellulose; D005947:Glucose