title: The influence of dopamine on active sodium transport across frog skin measured as short circuit current.
abstract: The influence of dopamine (DA) on short circuit current (SCC) across isolated Rana esculenta skin was studied to determine whethera a DA receptor system exists in that cell membrane model and what is the influence of DA on SCC. Experiments were carried out with both alpha- and beta-adrenergic blockers and cocaine present in the Ringer solution. DA in cumulative doses added to the inner Ringer solution stimulated SCC in a dose-dependent manner. Apomorphine (10(-5) M) shifted the DA dose--response curve to the left and increased the maximum DA response. Haloperidol (10(-9) M) antagonized the effects of the DA depressing maximum response as well. Imidazole (10(-8) M and 10(-9) M) antagonized the influence of DA in a manner similar to haloperidol. It appeared that there were DA receptors in frog skin and that haloperidol as well as imidazole were DA antagonists which acted non-competitively.
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001001:Anura; D001058:Apomorphine / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D004298:Dopamine / Q000494:pharmacology*; D018492:Dopamine Antagonists; D004305:Dose-Response Relationship, Drug; D006220:Haloperidol / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D066298:In Vitro Techniques; D011954:Receptors, Dopamine; D012867:Skin / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
chemical_list: D018492:Dopamine Antagonists; D007093:Imidazoles; D011954:Receptors, Dopamine; D012964:Sodium; D006220:Haloperidol; D001058:Apomorphine; D004298:Dopamine