Determination of prostaglandins and other products of arachidonate oxygenation in perfusates and during platelet aggregation.
Some analytical aspects of prostaglandin research are discussed. We present our latest results of the determination of the various products of arachidonate oxygenation in biological fluids and during platelet aggregation. These products were determined by (GLC) in combination with electron-capture detection or mass spectrometry (multiple-ion detection). The major component released from isolated perfused heart and lung was prostacyclin, determined as its hydrolysis product 6-oxo-PGF1alpha. Spleen released much thromboxane and monohydroxy acids. Normal rat platelets formed also much hydroxy acids during aggregation but at essential fatty acid deficiency, only very small amounts of product were detected.
D000818:Animals; D001095:Arachidonic Acids / Q000097:blood*; D006321:Heart / Q000502:physiology; D066298:In Vitro Techniques; D008168:Lung / Q000502:physiology; D010084:Oxidation-Reduction; D010477:Perfusion; D010974:Platelet Aggregation; D011453:Prostaglandins / Q000097:blood*; D011817:Rabbits; D051381:Rats
D001095:Arachidonic Acids; D011453:Prostaglandins