The Arrhenius plot behaviour of rat liver 5'-nucleotidase in different lipid environments.
The plasma membrane enzyme 5'-nucleotidase (EC 3.1.3.5) was prepared from rat liver as a complex with sphingomyelin or in detergent-solubilized forms. Each preparation exhibited a Km and Arrhenius break temperature indistinguishable from that of 5'-nucleotidase in plasma membranes. Measurement of fatty acid profiles, cholesterol and phospholipid content however showed a very wide variation between these preparations. We conclude that the biphasic nature of the Arrhenius plot of 5'-nucleotidase may be a property of the enzyme rather than its lipid environment.
D002462:Cell Membrane / Q000201:enzymology*; D002784:Cholesterol / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009708:Nucleotidases / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis; D013109:Sphingomyelins; D013696:Temperature
D005227:Fatty Acids; D010743:Phospholipids; D013109:Sphingomyelins; D002784:Cholesterol; D009708:Nucleotidases