title: Cryopreservation of lymphocytes to standardize in vitro immune functions.
abstract: The responses of fresh and frozen lymphocytes to mitogens and antigens have been compared with the use of samples collected on five occasions from 1 normal donor. The day-to-day variation seen with the fresh cells was eliminated by the use of frozen cells. Thawed cells from 1 donor, collected once but studied on five occasions and compared with fresh cells on the same days, fluctuated from day to day as well, confirming that the day-to-day variation seen is due to technical and not biologic phenomena. Cryopreserved cells showed a decrease in responses to specific microbial antigens, a slight shift in the PHA dose-response curve, but no significant difference in responses to Con A or PWM. The relative proportion of lymphocyte subpopulations changed with freezing and thawing. The proportion of T- and B-cells increased and decreased, respectively.
mesh_terms: D003208:Concanavalin A / Q000494:pharmacology; D005615:Freezing; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008934:Mitogens / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012015:Reference Standards
chemical_list: D008934:Mitogens; D010835:Phytohemagglutinins; D003208:Concanavalin A