title: Secretin/cholecystokinin-stimulated secretion of trypsinogen and trypsin inhibitor in pure human pancreatic juice collected by endoscopic retrograde catheterization.
abstract: Pure pancreatic juice was collected by endoscopic retrograde catheterization of the papilla of Vater from 10 fasting patients without known pancreatic disease. Secretin was given intravenously as a bolus dose at the beginning of the examination and cholecystokinin was given in the same way 15 min later. The juice was siphoned for 25 min and collected in 1-min fractions. Pancreatic secretory trypsin inhibitor, PSTI, and trypsinogen were measured by an immunochemical method. Secretin produced an increase in hydrogencarbonate and a decrease in chloride secretion. Cholecystokinin caused a prompt increase in the concentration of both PSTI and trypsinogen. The ratio between these was constant, suggesting a strictly parallel secretion. The effect of cholecystokinin on secretion of total protein when given as bolus dose was of short duration, with a half-life of action of about 3.5 min.
mesh_terms: D014670:Ampulla of Vater / Q000378:metabolism; D002404:Catheterization; D002766:Cholecystokinin; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D010189:Pancreatic Juice / Q000378:metabolism*; D012633:Secretin; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000378:metabolism*; D014362:Trypsinogen / Q000378:metabolism*
chemical_list: D014361:Trypsin Inhibitors; D012633:Secretin; D014359:Trypsin Inhibitor, Kazal Pancreatic; D014362:Trypsinogen; D002766:Cholecystokinin