Isolation and partial characterization of a mitochondrial deoxyribonucleic acid-protein complex from sea urchin embryos.
Lysis of mitochondria from sea urchin embryos with Triton X-100 led to a complete conversion of DNA-containing mitochondrial residues into protein-DNA complex with a density higher than 1.22 g/cm3 in sucrose solutions. This complex banded isopycnically in metrizamide gradients at a density of 1.26 g/cm3. Exposure to mixtures of Triton X-100 with Tween 80 resulted in progressively less delipitated and disorganized mitochondria over Tween/Triton weight ratios from 1 to 2, with the retention of the starting buoyant density in sucrose of approximately 1.16 g/cm3 at Tween/Triton ratios above 2.5. The DNA-internal protein complex sedimented with the bulk of the surviving mitochondrial structure under all conditions studied. No free DNA could be detected under any conditions of membrane removal.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D003852:Deoxyribonucleoproteins / Q000302:isolation & purification*; D003902:Detergents; D004625:Embryo, Nonmammalian / Q000032:analysis*; D007425:Intracellular Membranes / Q000032:analysis; D008928:Mitochondria / Q000032:analysis; D009698:Nucleoproteins / Q000302:isolation & purification*; D011485:Protein Binding; D012617:Sea Urchins; D013347:Subcellular Fractions
D004272:DNA, Mitochondrial; D003852:Deoxyribonucleoproteins; D003902:Detergents; D009698:Nucleoproteins