Establishment of mouse neuroblastoma clone N18, rat glioma clone C6, and rat striated muscle clone L6 in serum-free, chemically defined medium.
Mouse neuroblastoma clone N18, rat glioma clone C6, and rat striated muscle clone L6 were grown in a serum-free Dulbecco's modified Eagle's medium. Their doubling times were 48 hours, 40 hours, and 4 days, respectively. Morphologic features were similar to the original parent cell lines. Membrane components of N18 and C6 grown in serum-free medium were compared with the original parent cell lines. Defects of several membrane proteins were found with sodium dodecyl sulfate--polyacrylamide gel electrophoresis.
D000818:Animals; D002999:Clone Cells / Q000166:cytology; D003470:Culture Media; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000473:pathology; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D051381:Rats
D003470:Culture Media; D008565:Membrane Proteins