title: Biosynthesis of protein by the retina. The properties of a cell-free system obtained from rat retina.
abstract: We describe the properties of a post-mitochondrial (30,000 g) supernatant fluid obtained from rat retina which incorporated (3H) leucine into an alkali-stable acid-insoluble fraction (protein). It contained a substance, possibly leucine, which interfered with the incorporation of (3H) leucine into acid-insoluble fractions and which could be removed by passage through Sephadex G-25 or by dialysis. Magnesium was essential for protein synthesis and potassium enhanced it. Radioactive leucine was incorporated in the protein only when both adenosine triphosphate and guanosine triphosphate were present. Puromycin inhibited protein synthesis. The amount of material required for this assay is small. The system can be used to study changes in protein synthesis in the retinas of rats with retinal dystrophies.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002474:Cell-Free System; D003864:Depression, Chemical; D005136:Eye Proteins / Q000096:biosynthesis*; D007930:Leucine / Q000096:biosynthesis*; D008274:Magnesium / Q000494:pharmacology; D010725:Phosphocreatine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D012160:Retina / Q000378:metabolism* / Q000473:pathology; D012162:Retinal Degeneration / Q000378:metabolism* / Q000473:pathology; D013268:Stimulation, Chemical; D013347:Subcellular Fractions; D014316:Tritium
chemical_list: D005136:Eye Proteins; D010725:Phosphocreatine; D014316:Tritium; D011691:Puromycin; D000255:Adenosine Triphosphate; D007930:Leucine; D008274:Magnesium; D011188:Potassium