title: Expression of TEM-induced damage to postmeiotic stages of spermatogenesis of the mouse during early embryogenesis. II. Cytological investigations.
abstract: After treatment of postmeiotic stages of spermatogenesis of the mouse with TEM (0.2 or 0.4 mg/kg), dose- and stage-of-spermatogenesis-dependent frequencies of cytogenetic aberrations can be observed in early embryos. The frequencies of first-cleavage metaphases exhibiting structural aberrations (all of the chromosome type), the frequencies of 2 cell embryos and of 4--8 cell embryos containing nuclei accompanied by micronuclei or nuclei connected by bridges show a close correlation to frequencies of preimplantation loss of embryos recorded in a dominant lethal test. The frequencies of morulae/blastulae exhibiting blastomeres with micronuclei show a close correlation to the frequencies of total (pre- and post-implantation) loss of embryos. This indicates delayed expression of TEM-induced chromosomal damage which could persist undetected in very early stages of embryogenesis.
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D005260:Female; D005787:Gene Frequency; D005799:Genes, Dominant; D005804:Genes, Lethal; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009153:Mutagens; D013090:Spermatocytes / Q000187:drug effects*; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D014265:Triethylenemelamine / Q000494:pharmacology*
chemical_list: D009153:Mutagens; D014265:Triethylenemelamine