Purification of renal rat kallikrein and chemical relations with urinary rat kallikrein.
A highly purified kallikrein from rat kidney was obtained by a multi-step purification procedure including gel filtration on Sephadex G-100, chromatography on Whatman CM-32 and DE-32 celluloses and Sephadex G-100 superfine. Molecular weight of 40,000 was estimated by sodium dodecyl sulfate polyacrylamide disc gel electrophoresis. On the purified enzyme the aminoacid analysis was determined. Some chemical differences between renal kallikrein and the most abundant rat urinary kallikrein were illustrated.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004950:Esterases / Q000378:metabolism; D007610:Kallikreins / Q000302:isolation & purification* / Q000378:metabolism / Q000652:urine; D007668:Kidney / Q000032:analysis*; D008970:Molecular Weight; D051381:Rats
D000596:Amino Acids; D004950:Esterases; D007610:Kallikreins