[Activity alterations of unspecific esterases during wound healing. Investigations by means of electrofocusing (author's transl)].
Electrofocusing of skin-wound extracts on polyacrylamide flat gels is appropriate for zymographic separation of the esterase isoenzymes. In animal experiments and corpse material most fractions indicated non-synchronous, wound-age depending alterations of activity. As early as 15 minutes after incision obvious activity increases of A1-3-isoenzymes could be demonstrated, followed by a transitory decrease and another maximum. Important activities of the A4-isoenzymes became evident not before the 1st (human) resp. 3rd hour (guinea pig) after incision. There are no sufficient correlations between activity alterations and wound age, to allow a fairly exact time interval classification.
D000328:Adult; D000818:Animals; D004950:Esterases / Q000032:analysis*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D012867:Skin / Q000293:injuries; D013997:Time Factors; D014945:Wound Healing; D014947:Wounds and Injuries / Q000201:enzymology*
D007527:Isoenzymes; D004950:Esterases
Aktivitätsveränderungen der unspezifischen Esterasen im Wundheilungsprozess. Untersuchungen mit Hilfe der Elektrofokussierung.