A method for the evaluation of 3-hydroxyproline in the urine.
After hydrolysis of the urine by 6 M HCl, 3-hydroxyproline is purified from many interfering substances by 2 steps of chromatography on Dowex 1 X8 resin equilibrated first in the acetate form and secondly in the OH- form. The amino acid is finally evaluated by chromatography on Dowex 50 M82 resin in a Beckman multichrom amino acid analyzer. In 23 normal adult subjects the mean level was 12.5 +/- 3.5 mumol/24 h. In 8 normal children the level was 6.0 +/- 5 mumol/24 h and in 8 teenagers 15.2 +/- 2.85 mumol/24 h. The ratio of 3-hydroxyproline to 4-hydroxyproline seems indicative of a semiological value of this evaluation in cases of basement membrane collagen deterioration.
D000293:Adolescent; D000328:Adult; D002648:Child; D006801:Humans; D006909:Hydroxyproline / Q000652:urine*; D008722:Methods
D006909:Hydroxyproline