Detection and quantitation of adenovirus type 12 transforming DNA segments and their application in etiological studies of human neoplasia.
The oncogenic capacity of the tumour virus lies in a small region of the viral genome, and tumour cells and cells transformed by DNA tumour viruses often contain only a part of the viral genome, the portion including the transforming gene(s). The detection and quantitation of these gene(s) in tumour cell DNA can provide evidence for or against a possible etiological role of the virus in human neoplasia. In this communication, the isolation, purification and identification of the transforming segment of Ad 12 are described.
D000256:Adenoviridae / Q000235:genetics; D002460:Cell Line; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000302:isolation & purification*; D005814:Genes, Viral; D006801:Humans; D009369:Neoplasms / Q000209:etiology* / Q000235:genetics
D004279:DNA, Viral