Evaluation of immune response in chronically hemodialysed carriers of HB, antigen.
The presence of anti-HBs antibodies was studied by immunoelectroprecipitation and the presence of anti-HBc antibodies by an indirect immunofluorescence technique in sera of 15 HBs antigen (HBsAg) carriers chronically haemodialysed due to uremia, of whom 7 had suffered from acute viral hepatitis B. In the latter group, PHA-induced blast transformation of lymphocytes and leukocyte migration inhibition in the presence of HBsAg was estimated. In none of the cases could anti-HBs antibodies be detected while anti-HBc antibodies were present in all studied persons. Mitotic indices showed normal reactivity of lymphocytes to PHA. Except for 2 cases, the HBsAg carriers who once suffered from acute viral hepatitis B contained in their blood no HBsAg-sensitized lymphocytes. In 12 examined individuals ayw subtype of HBs was demonstrated and 3 persons had adw subtype.
D000917:Antibody Formation; D002464:Cell Migration Inhibition; D002908:Chronic Disease; D006510:Hepatitis B Antibodies / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D007111:Immunity, Cellular; D007676:Kidney Failure, Chronic / Q000276:immunology*; D008213:Lymphocyte Activation; D006435:Renal Dialysis
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens