title: An isochizomer of TaqI from Thermus thermophilus HB8.
abstract: A site-specific endonuclease has been isolated from Thermus thermophilus HB8 and named TthHB8I. It recognizes the same sequences as TaqI from Thermus aquaticus YT-1 does. The amount of TthHB8I in the cells was comparable to that of TaqI. T. thermophilus HB8 has an advantage over T. aquaticus YT-1 for preparation of a TaqI-like enzyme since it is easier to obtain T. thermophilus HB8 cells in quantity.
mesh_terms: D001665:Binding Sites; D003090:Coliphages; D004279:DNA, Viral / Q000378:metabolism; D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism*; D013824:Thermus / Q000201:enzymology*
chemical_list: D004279:DNA, Viral; D004720:Endonucleases