[Culture of normal and pathological human growth cartilage. Action of vitamin D derivatives and somatomedin].
Chondrocytes from normal cartilage of a 2 month old child have been cultured. In vitro the cells maintained their morphological identity and synthesised proteoglycans with electrophoretic properties similar to those synthesised by cartilage in vivo. Sulphation was stimulated by vitamin D metabolites and by somatomedin. This technique has been used to study biopsies of abnormal cartilage.
D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002807:Chondroitin / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007223:Infant; D011509:Proteoglycans / Q000378:metabolism; D013002:Somatomedins / Q000494:pharmacology*; D014807:Vitamin D / Q000378:metabolism*
D011509:Proteoglycans; D013002:Somatomedins; D014807:Vitamin D; D002807:Chondroitin
Culture de cartilage de croissance humain normal et pathologique. Action de dérivés de la vitamine D et de la somatomédine.