Hydrostatic pressure effects upon cellular leakage and active transport by Vibrio marinus.
Hydrostatic pressures greater than ca. 400 atm cause release of cellular components of Vibrio marinus MP-1 in the order protein greater than RNA greater than malate dehydrogenase greater than DNA greater than amino acids. Increased pressures also slow the rate of cycloleucine (non-metabolizable analogue of L-leucine acid L-valine) uptake but increase its efflux rate. Lineweaver-Burk plots (1/V versus 1/[S]) of cycloleucine uptake indicate that the Km increases with compression which suggests a decrease in affinity of the transport system for substrate at increased pressures.
D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001693:Biological Transport, Active; D003515:Cycloleucine / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D006874:Hydrostatic Pressure; D008291:Malate Dehydrogenase / Q000378:metabolism; D011312:Pressure; D012329:RNA, Bacterial / Q000378:metabolism; D012623:Seawater; D014733:Vibrio / Q000378:metabolism*; D014871:Water Microbiology
D000596:Amino Acids; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D003515:Cycloleucine; D008291:Malate Dehydrogenase