title: Cytotoxic effects of toximul MP8 in human lymphocytes in culture: a preliminary investigation.
abstract: Toximul MP8, a commercial emulsifier formulation, has been used for a number of years to spray forest trees infested with spruce budworm with the organophosphate insecticides, Fenitrothion and Phosphamidon. To assess its cytotoxic effects on human lymphocytes varying amounts of Toximul MP8 were added to human whole blood (0--50 ppm) and cultured along with phytohaemagglutinin for 52h. At the end of the culture period cytotoxicity to human lymphocytes was assessed by (1) mitotic index, (2) DNA labelling index and (3) the frequency of chromatid breaks. The cytotoxic effects were apparent even at the lowest concentration tested, i.e., 10 ppm. A significant dose-dependent decrease in the mitotic and DNA-labelling indicies was observed. Toximul MP8 also produced a significant level of chromatid breaks even at 10 ppm.
mesh_terms: D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008940:Mitotic Index; D009153:Mutagens; D009930:Organic Chemicals
chemical_list: D004655:Emulsions; D009153:Mutagens; D009930:Organic Chemicals; C019116:Toximul MP8