Purification of pancreatic lipase via its affinity for bile salts and apolar surfaces.
An improved method for the purification of pancreatic lipase in milligram amounts is described. Only one batch step and one chromatographic step are required to produce a lipase-colipase complex and a lipase largely free of colipase. Both species are lipid free; physical and kinetic data indicate that the predominant lipase species is lipase B.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D003089:Colipases / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification* / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology*; D013657:Taurodeoxycholic Acid
D003089:Colipases; D005227:Fatty Acids; D013657:Taurodeoxycholic Acid; D008049:Lipase