The aggregational status of cholera enterotoxin fragment A following biochemical fractionation.
Aggregates of frabment A of Vibrio cholerae enterotoxin were revealed following isoelectric focusing in 8 M urea of molecular sieve chromatography in 4% (v/v) formic acid. These aggregates consisted of dimers which required the presence of 10 M urea, 1% sodium dodecyl sulfate (SDS), 2 mM ethylenediaminetetracetic acid (EDTA) and heat (60 degrees C for 1 h) for complete dissociation. All aggregates were homogeneous when tested by standard analytical and SDS polyacrylamide gel electrophoresis or immunodiffusion analysis. Aggregates of fragment A were biologically active in the mouse Y1 adrenal cell assay.
D001681:Biological Assay; D002772:Cholera Toxin / Q000032:analysis*; D007526:Isoelectric Point; D046911:Macromolecular Substances; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D013329:Structure-Activity Relationship
D046911:Macromolecular Substances; D010455:Peptides; D002772:Cholera Toxin