Concanavalin A binding sites on the luminal surface of ependymal cells of third ventricle.
Detection of the Concanavalin A binding sites within the luminal surface of ependymal cells of the third ventricle has been accomplished using perfusion techniques as a way of exposing the cells to reaction solutions. Parameters for this procedure were established. It was found that electron dense reaction products form a layer ranging from 25--40 nm in thickness on the luminal surface of investigated cells. The microvilli exhibited a slightly thiner layer, the reaction products formed small patches on cilia.
D000818:Animals; D001665:Binding Sites; D003208:Concanavalin A / Q000494:pharmacology*; D004805:Ependyma / Q000648:ultrastructure*; D006352:Heart Ventricles / Q000648:ultrastructure; D006735:Horseradish Peroxidase / Q000494:pharmacology; D008297:Male; D051381:Rats
D003208:Concanavalin A; D006735:Horseradish Peroxidase