title: Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro.
abstract: Cells from the established cell line NHIK 3025 were synchronized by repeated mitotic selections. Survival of the synchronized cells after treatment with haematoporphyrin and near-UV light was measured by testing the capacity of the cells to form macroscopic colonies. The sensitivity to photodynamic inactivation was small in early G1, late S and G2. The sensitivity increased throughout late G1 and early S to a maximum in mid S. More than a 100-fold variation is found in the survival after 20 min irradiation in the presence of 4 X 10(-4)M haematoporphyrin.
mesh_terms: D002453:Cell Cycle; D002460:Cell Line; D006415:Hematoporphyrins / Q000494:pharmacology*; D008938:Mitosis / Q000528:radiation effects; D010778:Photochemotherapy; D014466:Ultraviolet Rays
chemical_list: D006415:Hematoporphyrins