[Study of Vibrio cholerae fractions. II. A comparison of the immunochemical and biological properties of preparations of alkaline extracts (vibrionin) and the endotoxin of Vibrio cholerae].
Comparative study of immuno-chemical and biological properties of vibrionin and cholera vibrio endotoxin showed vibrionin to contain up to 73.5% of protein, whereas endotoxin preparations contained only 25% of protein. Use of disc electrophoresis in polyacrylamide gel permitted to determine the relative electrophoretic mobility of the protein component bands of both preparations. Residual quantities of O-antigenic cholera vibrio determinants were revealed serologically in the vibrionin preparations. Study of biological properties of vibrionin (cholera allergen) demonstrated a possibility of using vibrionin in the capacity of a new bacterial allergen.
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001688:Biological Products / Q000032:analysis / Q000276:immunology* / Q000633:toxicity; D002458:Cell Fractionation; D004731:Endotoxins / Q000032:analysis / Q000276:immunology* / Q000633:toxicity; D006168:Guinea Pigs; D007114:Immunization; D007928:Lethal Dose 50; D051379:Mice; D014734:Vibrio cholerae / Q000276:immunology*
D001426:Bacterial Proteins; D001688:Biological Products; D004731:Endotoxins
Izuchenie fraktsiĭ kholernogo vibriona. Soobshchenie II. Sravnenie immunokhimicheskikh i biologicheskikh svoĭstv preparatov shchelochnykh ékstraktov (vibrionina) i éndotoksina kholernogo vibriona.