Transcription of the transforming region of adenovirus type 5 in a rat cell line transformed by a small restriction fragment.
The fraction of the adenovirus type 5 genome expressed as mRNA in a rat cell line, 5RK, transformed by the HsuI-G restriction fragment was analyzed by saturation hybridization using separated strands of 32P-labeled HindIII-G fragment. It was found that 45 to 50% of G fragment (about 8 X 10(5) daltons) is expressed as mRNA. The size of polyadenylic acid-terminated virus-specific cytoplasmic and nuclear RNA was determined by electrophoresis on polyacrylamide gels containing 98% formamide, followed by hybridization of gel slices with viral DNA. The major virus-specific RNA species present in the cytoplasm is about 14S, whereas the major virus-specific RNA present in the nucleus is 30 to 32S. The large size of nuclear virus-specific RNA suggests that host sequences are covalently linked to the viral transcript.
D000260:Adenoviruses, Human / Q000235:genetics*; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D002472:Cell Transformation, Viral; D003593:Cytoplasm / Q000032:analysis; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D012367:RNA, Viral