[Determination of the concentration of free magnesium ions in hemolysates of oxygenated and deoxygenated packed human erythrocytes].
Concentrations of ionized magnesium in hemolyzates of oxygenated and deoxygenated human erythrocytes were measured directly by means of an ion exchange method. At total concentrations of hemoglobin of 8 mmol/l H2O and total magnesium of 3.6 mmol/l H2O, mean values of [Mg2+] were found to be 0.45 in the oxygenated state and 0.57 mmol/l H2O in the deoxygenated state. The difference of 0.12 +/- 0.1 mmol/l H2O is considerably smaller than corresponding literature data obtained from equilibrium calculation.
D004912:Erythrocytes / Q000032:analysis*; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D007477:Ions; D008274:Magnesium / Q000097:blood*; D010100:Oxygen / Q000097:blood*
D007477:Ions; D008274:Magnesium; D010100:Oxygen
Bestimmung der Konzentrationen freier Magnesiumionen in Hämolysaten oxygenierter und desoxygenierter gepackter menschlicher Erythrozyten.