Studies of bile acid secretion by isolated rat hepatocytes.
Bile acid secretion was studied in isolated rat hepatocytes cultured in medium supplemented with cofactors and succinate. Succinate as a respiratory substrate was found to be essential for bile acid synthesis. Feedback mechanism was demonstrated with different inhibitory potency of different bile acids on bile acid synthesis in isolated hepatocytes.
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D002793:Cholic Acids / Q000378:metabolism; D005943:Gluconeogenesis; D066298:In Vitro Techniques; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D013656:Taurocholic Acid / Q000378:metabolism
D001647:Bile Acids and Salts; D002793:Cholic Acids; D007773:Lactates; D011773:Pyruvates; D013656:Taurocholic Acid