Purification of influenza A virus and preliminary characterization of virus proteins by polyacrylamide gel electrophoresis (PAGE).
Influenza A/ZSSR/053/74 (H3N2) from egg ellantoic fluid was partially purified by absorption and elution on chick erythrocytes and chromatography on Sepharose 2B. Concentrated and partially purified preparation of virus containing 5000 HAV and 80 micrograms of protein per ml was subjected to PAGE in 0.1% SDS. Eleven protein bands were selected of which three were identified as NA, NP and HA.
D004591:Electrophoresis, Polyacrylamide Gel; D009980:Influenza A virus / Q000032:analysis / Q000302:isolation & purification*; D008970:Molecular Weight; D014764:Viral Proteins / Q000032:analysis*
D014764:Viral Proteins