title: The influence of vinblastine and colchicine on renin secretion in vivo and in vitro.
abstract: The effect of the microtubule inhibitors colchicine and vinblastine on renin release in vivo and in vitro was studied. Injection of 0.5 mg/100 g i.v. of colchicine or vinblastine to furosemide treated rats on a low salt diet resulted in a decrease of plasma renin as well as plasma angiotensinogen concentration during a 5 h observation period. Renin release from rat kidney slices was diminished by vinblastine (5 x 10(-5) M), when basal or stimulated (by isobutylmethylxanthine and isoproterenol) renin release was measured. Colchicine at 5 x 10(-5) M had no effect under these conditions. Renin release from the isolated perfused rat kidney was increased 2--3 fold by vinblastine (10(-5) M) or colchicine (10(-4) M). The maximal response of renin release to isoproterenol (10(-7) M) was not changed when vinblastine (10(-5) M) or colchicine (10(-4) M) were present in the perfusion medium. The contrasting results cast considerable doubts on the suitability of microtubule inhibitors in studies on renin secretion.
mesh_terms: D000808:Angiotensinogen / Q000097:blood; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology*; D004039:Diet, Sodium-Restricted; D066298:In Vitro Techniques; D007606:Juxtaglomerular Apparatus / Q000502:physiology*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008870:Microtubules / Q000502:physiology; D010477:Perfusion; D051381:Rats; D012083:Renin / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology*
chemical_list: D000808:Angiotensinogen; D012965:Sodium Chloride; D014747:Vinblastine; D012083:Renin; D003078:Colchicine; D002118:Calcium