title: The effects of enzymes on the blood factors associated with the FV system of bovine erythrocytes.
abstract: Treatment of cattle red cells (CRC) with proteases and neuraminidase revealed at least one trypsin- or pronase-sensitive type and a protease-resistant type of sialoglycoprotein. The antigen F itself and the antigen IM in the F/F and F/V type of CRC were associated with the protease-resistant glycoprotein. Moreover, they were demonstrable in the agglutination test, but only after treatment with proteases. The antigen V, allelic to F, was, however, associated with a pronase-sensitive protein and the antigen IM in the V/V type of CRC with a trypsin- or pronase-sensitive glycoprotein. The antigen N' was inactivated by chymotrypsin or pronase, irrespective of whether it was transmitted with the F or with the V. While the antigens IM, N' and the antigen reacting with rabbit serum, in addition to the antigen F in the F/F type of CRC, showed no or only very weak (titre 1:2) reactions, these same antigens, in addition to the antigen V in the V/V type of CRC, all showed high titres (1:32 to 1:128) in the anti-globulin test.
mesh_terms: D000818:Animals; D001789:Blood Group Antigens / Q000235:genetics / Q000276:immunology*; D002417:Cattle / Q000097:blood* / Q000235:genetics; D004912:Erythrocytes / Q000378:metabolism; D005799:Genes, Dominant; D009439:Neuraminidase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism*
chemical_list: D001789:Blood Group Antigens; D012795:Sialoglycoproteins; D009439:Neuraminidase; D010447:Peptide Hydrolases