[Possibility of using enriched glial and neuronal cell fractions to study transport of neurotransmitter amino acids in old age].
Calculated on a protein basis the yield of neuronal and glial cell-enriched fractions obtained from the brain of aged and adult rats was found to be equal. In aged animals the high affinity uptake of 14C-GABA by neuronal fractions is reduced by 27.34% while that of glial fractions by 11.87%. At 0 degrees both age groups studied the uptake of 14C-GABA is greatly inhibited--by 66% in neurones and 92% in glia. The conclusion is drawn that glial cells are changed much less with age than neurones and thus their role as modulators of neuronal function increases with age.
D000375:Aging; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid
O vozmozhnosti ispol'zovaniia obogashchennykh fraktsiĭ kletok glii i neĭronov v izuchenii transporta neĭrotransmitternykh aminokislot v starcheskom vozraste.