[Isolation of hepatitis B (HBSAg) from donor blood plasma].
An original method for purification of HBsAg from the blood plasma of donors is described. Preparations have been obtained containing structures 22 nm in size the proteins of which form 9 bands in polyacrylamide gel electrophoresis and containing no detectable amounts of admixtures accompanying HBsAg. The purified HBsAg meets the requirements for test-antigens for radioimmuniassay and immune autoradiography. The reported method may be used for preparation of HBsAg under production conditions.
D002500:Centrifugation, Isopycnic; D002586:Cesium; D002712:Chlorides; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006509:Hepatitis B / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification*; D006801:Humans; D007122:Immunoelectrophoresis; D008722:Methods; D011863:Radioimmunoassay; D013395:Sucrose
D002712:Chlorides; D006514:Hepatitis B Surface Antigens; D002586:Cesium; D013395:Sucrose
Vydelenie poverkhnostnogo antigena gepatita B (HBSAg) iz plazmy krovi donorov.