[Determination of hexokinase isoenzymes in different tissues].
A modified method is described for estimation of hexokinase isoenzymes by polyacrylamide gel electrophoresis. The best conditions for detection of hexokinase activity were developed using immobilization of glucose-6-phosphate dehydrogenas: in gel, utilization of riboflavin as a catalyst of polymerization and stabilization by glucose. The method is simple, highly reproducible, comparatively rapid and economically reasonable.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004800:Enzymes, Immobilized; D005947:Glucose; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis*; D012256:Riboflavin
D004800:Enzymes, Immobilized; D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D005947:Glucose; D012256:Riboflavin
Ob opredelenii izofermentov geksokinazy v razlichnykh tkaniakh.