The kinetics of E. coli RNA polymerase.
Using an assay specific for chain elongation of E. coli RNA polymerase the kinetics of this propagation reaction have been studied. The kinetic behaviour is consistent woth the mathematical model formulated for this multisubstrate enzyme. The effect of increasing salt concentration on the kinetics of the reaction indicated that DNA unwinding is probably a necessary step in the propagation step, although this may not be the rate limiting step under all conditions.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D003597:Cytosine Nucleotides / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D007700:Kinetics; D008954:Models, Biological; D011189:Potassium Chloride / Q000494:pharmacology; D012329:RNA, Bacterial / Q000096:biosynthesis; D013696:Temperature; D014500:Uracil Nucleotides / Q000494:pharmacology
D003597:Cytosine Nucleotides; D004279:DNA, Viral; D012329:RNA, Bacterial; D014500:Uracil Nucleotides; D011189:Potassium Chloride; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D004247:DNA