title: Competition of some biogenic amines for uptake into synaptic vesicles of the striatum.
abstract: Synaptic vesicles were isolated from the caudate nucleus of the pigs by differential centrifugation and incubated with labelled monoamines in the absence or in the presence of ATP-Mg(2+). Addition of ATP-Mg(2+) enhanced the uptake of (14)C-dopamine into the vesicles. Serotonin competitively inhibited the ATP-Mg(2+)-dependent uptake of (14)C-dopamine without influencing the uptake which took place in the absence of ATP-Mg(2+). Likewise, dopamine caused a dose-dependent inhibition of the ATP-Mg(2+)-dependent uptake of (14)C-serotonin without inhibiting the uptake in the absence of ATP-Mg(2+). Incubation of the vesicles with equal concentrations of(3)-dopamine and (14)C-serotonin revealed that the presence of the one amine competitively inhibited the ATP-Mg(2+)-dependent uptake of the other. Tyranimine competitively inhibited the ATP-Mg(2+)-dependent uptake of (14)C-dine, (14)C-serotonin and (14)C-noradrenaline into the vesicles; the uptake of the amines which took place in the absence of ATP-Mg(2+) was not impairedby tyramine. Analysis of the amine uptake by the ABC test showed that a mutual inhibition exists between dopamine and serotonin for the uptake into the synaptic vesicles. GABA did not influence the uptake of (14)C-dopamine either in the absence, or in the presence of ATP-Mg(2+)...
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D001679:Biogenic Amines / Q000378:metabolism*; D001692:Biological Transport; D002421:Caudate Nucleus / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D009655:Octopamine / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013552:Swine; D013572:Synaptic Vesicles / Q000378:metabolism*; D014439:Tyramine / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D001679:Biogenic Amines; D009655:Octopamine; D012701:Serotonin; D005680:gamma-Aminobutyric Acid; D000255:Adenosine Triphosphate; D008274:Magnesium; D004298:Dopamine; D009638:Norepinephrine; D014439:Tyramine