Production of antibody against T-2 toxin.
Antibody against T-2 toxin was obtained after immunization of rabbits with bovine serum albumin-T-2 hemisuccinate conjugate. The antibody had greatest binding efficiency for T-2 toxin, less efficiency for HT-2, and least for T-2 triol. Cross-reaction of antibody with neosolaniol, T-2 tetraol, and 8-acetyl-neosolaniol was very weak. Diacetoxyscirpenol, trichodermin, vomitoxin, and verrucarin A essentially gave no cross-reaction with the antibody. The sensitivity of the binding assay for T-2 toxin detection was in the range of 1 to 20 ng per assay. Detailed methods for the preparation of the conjugate and the production of immune serum and methods for antibody determination are described.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D055598:Chemical Phenomena; D002621:Chemistry; D003429:Cross Reactions; D007114:Immunization; D011817:Rabbits; D012717:Sesquiterpenes / Q000276:immunology*; D013605:T-2 Toxin / Q000276:immunology*; D014255:Trichothecenes / Q000276:immunology
D012717:Sesquiterpenes; D014255:Trichothecenes; D013605:T-2 Toxin