title: A novel nucleotide implicated in the response of E. coli to energy source downshift.
abstract: When E. coli cells are subjected to energy source downshift, the accumulation of RNA (and overall cell growth) is drastically restricted within 1 to 2 min. However, the identity of the primary metabolic signal for this adjustment is a mystery. Earlier studies, and further evidence presented here, show that there is no satisfactory correlation between the sudden adjustment of RNA accumulation and the kinetics of changes in the levels of prospective signalling compounds, such as glycolytic intermediates, ppGpp, ATP, or the three adenylate nucleotides. We have discovered an unusual nucleotide, which we call the phantom spot, whose level decreases dramatically within a minute of downshift, correlating well with the adjustment of RNA accumulation. Preliminary characterization of the phantom spot indicates that it is a triphosphate derived from the guanylate pathway, and suggests that it is a form of GTP with a modification of the imidazole portion of the purine ring. We postulate that this nucleotide serves as a regulatory facsimile of ATP, linking the rate of RNA accumulation and other anabolic processes to the overall rate of phosphorylation.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005947:Glucose; D006019:Glycolysis; D006150:Guanine Nucleotides / Q000378:metabolism*; D006151:Guanosine / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis; D013386:Succinates
chemical_list: D001426:Bacterial Proteins; D006150:Guanine Nucleotides; D012329:RNA, Bacterial; D013386:Succinates; D006151:Guanosine; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D005947:Glucose