[Genetic control of the RecF recombination pathway. II. Effect of recL- and uvrE- mutations].
It was found earlier that the effect of the uvrE- mutation on the yield of recombinants formed via RecF pathway of recombination was mainly due to the decreased viability of the recB-C-sbcB-uvrE- recipient. The results of the present paper show the uvrE- and recL- mutations reduce the recipient ability of the recB-C- sbcB- strains to the same extent. We were unable to find any evidence for complementation between the recL- and uvrE- mutations in transient as well as in stable uvrE-/recL- merozygotes. The recL152 as well as the uvrE502 mutation is lethal for the strains deficient in DNA polymerase I. Both recL- and uvrE- mutations reduce in the recB-C- sbcB- strains the probability of inheritance of the proximal non-selective donor markers as well as selective markers located close to the leading end of the donor chromosome. The former effect however could not be completely due to the latter.
D000483:Alleles; D004926:Escherichia coli / Q000502:physiology*; D009154:Mutation; D011995:Recombination, Genetic
Izuchenie geneticheskogo kontrolia RecF-puti rekombinatsii. Soobshchenie II. Effekt mutatsiĭ recL- i uvrE-