[Origin of stromal mechanocytes in the bone marrow cell cultures].
The method of indirect immunofluorescence with the use of the isolinear antiserum was applied to the study of the linear reference of fibroblasts growing in the monolayer bone marrow cultures from the semisyngenous heterotropic transplants and radiochimerae. As shown, fibroblasts from the bone marrow of complete radiochimerae were of the recipient origin, whereas the histiocytes-macrophages (in the same cultures) - of donor origin. All the fibroblasts in the bone marrow cultures of the heterotropic transplant belonged to the initial donor, whereas 80% of the histiocytes were recipent's. This is a direct proof of the fact that stromal mechanocytes and hemopoietic cells, and also macrophages belonged to different cell lins.
D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002460:Cell Line; D046508:Culture Techniques; D005347:Fibroblasts / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D006644:Histiocytes / Q000166:cytology*; D051379:Mice; D011828:Radiation Chimera; D014184:Transplantation, Homologous
Proiskhozhdenie stromal'nykh mekhanotsitov v kul'turakh kostnogo.