Membrane immunoglobulin on mouse myeloma cells: discrepancies in the detection of membrane IG by two different methods.
Two different methods, immunofluorescence and lactoperoxidase-catalysed radioiodination, were used to investigate mIg on two different myeloma cell lines. The data indicate that, even though both techniques are highly sensitive, different results can be obtained in the same cell line depending on the technique employed.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis*; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D007784:Lactoperoxidase; D008722:Methods; D051379:Mice; D009194:Myeloma Proteins / Q000302:isolation & purification*; D011947:Receptors, Antigen, B-Cell
D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D009194:Myeloma Proteins; D011947:Receptors, Antigen, B-Cell; D007784:Lactoperoxidase