A method for biological testing of containment systems for viral agents.
A technique utilizing coliphage as the test material has been developed and employed to evaluate the effectiveness of a containment system for zonal centrifugation of hepatits viruses. An Andersen Viable Particle Sampler which had been loaded with plates containing a base layer of agar nutrient with an overlay of E. coli- agar suspension was used to sample the test air. The containment system, which includes a HEPA filter, was challenged with an aerosolized suspension of coliphage.
D000391:Air Microbiology; D001431:Bacteriological Techniques / Q000295:instrumentation*; D002501:Centrifugation, Zonal; D003090:Coliphages / Q000302:isolation & purification*; D004864:Equipment and Supplies / Q000592:standards; D005069:Evaluation Studies as Topic; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007757:Laboratory Infection / Q000517:prevention & control; D008722:Methods