title: Uteroglobin in the rabbit. II. Intracellular localization in the uterus after hormone treatment.
abstract: Rabbit uterine uteroglobin (UGL) was studied by electrophoretic and immunological methods following normal copulation, after ovariectomy and progesterone treatment, 17beta-oestradiol and combined progesterone treatment, 17 beta-oestradiol treatment alone and after HCG-induced pseudopregnancy. Electrophoretic studies show the amount of ULG in uterine secretions, the immunological investigations indicate the intracellular localization of ULG and the distribution of ULG-positive cells in the endometrium. No obvious differences were found between the uteri 7 days after injection with chorion-gonadotropin and those 7 days following normal copulation. No differences could be domonstrated between the uteri of animals 35 days following ovariectomy and subsequent progesterone treatment on Days 31-33 and those of normal 7 d. post coitum (p.c.) animals. Uteri from animals treated with progesterone on Days 2-5 p.c. contained more ULG-positive cells than controls. 17beta-oestradiol treatment with and without subsequent progesterone treatment resulted, in both gravid and ovariectomized animals, in the formation of a tall columnar endometrial epithelium. Treatment with 17 beta-oestradiol on Days 1 and 2 p.c. led to a decrease in the number of UGL-positive cells at 7 days p.c. Even after ovariectomy with 17 beta-oestradiol substitution, UGL-positive cells were still present in the endometrium. However a secretion of any magnitude could not be detected. The importance of differentiation between synthesis and secretion (= release) as distinct phases of the glandular response is especially emphasised by the latter findings.
mesh_terms: D000818:Animals; D002369:Castration; D006063:Chorionic Gonadotropin; D003307:Copulation; D004586:Electrophoresis; D004717:Endometrium / Q000032:analysis; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D007158:Immunologic Techniques; D011374:Progesterone / Q000494:pharmacology*; D011555:Pseudopregnancy; D011817:Rabbits; D013997:Time Factors; D014598:Uteroglobin / Q000032:analysis*; D014599:Uterus / Q000032:analysis*
chemical_list: D006063:Chorionic Gonadotropin; D006023:Glycoproteins; D011374:Progesterone; D004958:Estradiol; D014598:Uteroglobin