title: DNA Degradation and reduced recombination following UV irradiation during meiosis in yeast (Saccharomyces cerevisiae).
abstract: Irradiation of meiotic yeast cells with moderate doses of ultraviolet irradiation (1,600 erg/mm2) leads to the arrest of premeiotic DNA synthesis, massive (5-40%) DNA degradation, and a 40-50% loss of cell viability. In contrast, such doses of UV irradiation had a minor effect on viability (15-20% loss) of logarithmically growing cells, and no comparable DNA degradation was observed in irradiated synchronized vegetative cells. Meiotic recombination is also affected by UV irradiation. When administered at a stage comparable to meiotic prophase, low doses of irradiation result in a reduction in recombination frequency without significantly affecting cell viability.
mesh_terms: D004247:DNA / Q000096:biosynthesis / Q000378:metabolism / Q000528:radiation effects*; D005838:Genotype; D008540:Meiosis / Q000528:radiation effects*; D011831:Radiation Genetics; D011995:Recombination, Genetic / Q000528:radiation effects*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D004247:DNA