Simple assay for 5-fluorocytosine in the presence of amphotericin B.
A simple method for the measurement of 5-fluorocytosine in the presence of amphotericin B is described. The antifungal activity of amphotericin B is abolished by heating serum at 100 C for 45 min. 5-Fluorocytosine is unaffected by this treatment, and serum levels can be subsequently assayed by either tube dilution or disk diffusion methods.
D000666:Amphotericin B / Q000097:blood / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods*; D002176:Candida albicans / Q000187:drug effects; D002177:Candidiasis / Q000097:blood; D003453:Cryptococcosis / Q000097:blood; D003596:Cytosine / Q000031:analogs & derivatives*; D005437:Flucytosine / Q000097:blood* / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D008581:Meningitis / Q000097:blood; D012441:Saccharomyces cerevisiae / Q000187:drug effects
D000666:Amphotericin B; D003596:Cytosine; D005437:Flucytosine