title: The 5' ends of yeast killer factor RNAs are pppGp.
abstract: The 5' nucleotides of the double-stranded RNAs of yeast killer factor have been isolated by digestion with pancreatic, T1 and T2 RNase followed by two-dimensional electrophoresis. They were identified by bacterial alkaline phosphatase and snake venom phosphodiesterase digestions. Both the larger double-stranded RNA (L, of 2.5 x 10(6) daltons) and the smaller double-stranded RNA (M, of 1.4 x 10(6) daltons) have the 5' end groups pppGp. These 5' ends are dissimilar to those of the double-stranded RNAs of animal viruses but may be characteristic of the 5' ends of the double-stranded RNAs of fungal viruses.
mesh_terms: D006150:Guanine Nucleotides / Q000032:analysis*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000032:analysis* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000378:metabolism / Q000502:physiology
chemical_list: D006150:Guanine Nucleotides; D012333:RNA, Messenger; D012313:RNA