title: Inhibitory and augmentative effects of oestradiol on LH-RH-induced release of LH by anterior pituitary glands from intact female rats in vitro.
abstract: Anterior pituitary glands from intact dioestrous female rats were incubated in vitro and the LH released into the medium was measured at hourly intervals. The effect of a supramaximally active dose of LH-RH was first inhibited and later augmented by oestradiol added to the medium. The augmented response also occurred when LH-RH was absent during the preceding phase of the incubation. Both effects of oestradiol could be blocked by cycloheximide, which agent, however, also partly inhibited the response to LH-RH.
mesh_terms: D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004031:Diestrus / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007700:Kinetics; D007986:Luteinizing Hormone / Q000097:blood*; D010902:Pituitary Gland / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D007986:Luteinizing Hormone; D003513:Cycloheximide