[Comparative enzymatic study of two strains of Aspergillus fumigatus and of A. fischeri of sapropytic origin or isolated from human or animal lesions: practical diagnostic applications].
14 enzymatic activities (3 dehydrogenases and 11 hydrolases) were systematically investigated in the extracts of 2 strains of Aspergillus fischeri and 14 strains of A. fumigatus (8 isolated from human or animal lesions named 'pathogenic' strains and 6 strains of saprophytic origin). The enzymic composition of A. fumigatus appeared to be very different from one strain to another but no relationships could be established between enzymic differences and origin of the strains. In addition, the immunoelectrophoretic analysis of these antigenic extracts using rabbits immunsera and sera of patients with pulmonary asperigllosis was performed. The type II chymotrypsic activity, corresponding to C arc, was shown more frequently and gave stronger reaction when antigenic extracts from pathogenic strains were allowed to react with sera of patients. Moreover, when sera of patients revealed a same enzymic activity in a saprophytic strain and a pathogenic strain, the number of precipitin lines was significantly larger with the latter one. Accordingly, antigenic extracts used for precipitin tests had to be preferably prepared from pathogenic strains and tested, for value, against sera of patients with pulmonary aspergillosis.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001228:Aspergillosis / Q000201:enzymology; D001230:Aspergillus / Q000201:enzymology*; D001232:Aspergillus fumigatus / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D007122:Immunoelectrophoresis; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D011233:Precipitin Tests; D013045:Species Specificity
D010088:Oxidoreductases; D008291:Malate Dehydrogenase; D002374:Catalase; D006867:Hydrolases; D002265:Carboxylic Ester Hydrolases; D000469:Alkaline Phosphatase; D005959:Glucosidases; D010447:Peptide Hydrolases; D007931:Leucyl Aminopeptidase; D002918:Chymotrypsin
Etude enzymologique comparee de souches de Aspergillius fumigatus et de A. fischeri d'origine saprophytique ou isolees de lesions humaines ou animales. repercussions pratiques d'ordre diagnostique