[Hexokinase activity in the soluble fraction of the nucleus and cytoplasm in cell cultures infected with human adenoviruses types 6 and 12].
A study was made of the activity of hexokinase in the soluble fraction of the nucleus and cytoplasm of fibroblasts of rat embryos (primary cultures) during contact with infectious (A6) and oncogenic (A12) human adenoviruses. A high activity of the enzyme was seen in the cytoplasmic fraction in comparison with the nuclear one both in the infectious adrenovirus of type 6 this value differed from the normal and in the experimental samples. With the action of the normal but insignificantly. Marked changes in the enzyme activity were observed in case of cell contact with the oncovirus. They occurred both in the nucleus and in the cytoplasm the first days of the virus inculcation, increasing gradually with the lapse of tiay; these disturbances were much greatert in the cytoplasm than in the nucleus. It is supposed that the changes revealed possibly associated with the impossibility to regulate the cell proliferation process.
D000256:Adenoviridae / Q000254:growth & development*; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology*; D003593:Cytoplasm / Q000201:enzymology*; D006593:Hexokinase / Q000378:metabolism*; D006666:History of Medicine; D009858:Oncogenic Viruses / Q000254:growth & development; D014779:Virus Replication
D006593:Hexokinase
Aktivnost' geksokinazy v rastvorimoĭ fraktsii iadra i tsitoplazme kultur kletok, zarazhennykh adenovirusami tipov 6 i 12