title: Structure and function of chloroplast-type ferredoxins.
abstract: Comparison of various chloroplast-type ferredoxin sequences, chemical and enzymic modifications, reconstitution experiments, and fluorescence measurement of chloroplast-type ferredoxins have led to the following conclusions. 1. Tyrosine, histidine, and tryptophan residues are not directly involved in the oxidation-reduction mechanism of ferredoxins. The four indispensible cysteine residues in spinach ferredoxin which constitutes a part of the iron-sulfur cluster are located at residues 39, 44. 47 and 77. Two out of six cysteine residues in Spirulina ferredoxin could be easily modified with vinylpyridine without the loss of reconstitutive ability i.e. the apoferredoxin could be converted to the holoform by the addition of iron and sulfide. 2. Spinach ferredoxin was digested with carboxypeptidase A and the terminal alanine could be removed without loss of the spectral properties of native ferredoxin. However, the removal of the terminal three residues gave rise to the loss of reconstitutive ability. 3. The amino groups of spinach ferredoxin were modified by acetic anhydride and four residues were acetylated. The acetylated preparation of ferredoxin had an unique spectrum. Upon the addition of high concentration of ions the spectrum of this derivative resembled the spectrum of native ferredoxin. Acetylferredoxin did not combine with ferredoxin-NADP reductase, but upon the addition of moderate concentrations of cations, it did bind to this enzyme.
mesh_terms: D000595:Amino Acid Sequence; D001120:Arginine / Q000032:analysis; D002268:Carboxypeptidases; D002736:Chloroplasts / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism; D008274:Magnesium; D009994:Osmolar Concentration; D011487:Protein Conformation; D012964:Sodium; D013045:Species Specificity; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D014364:Tryptophan / Q000032:analysis
chemical_list: D005288:Ferredoxins; D014364:Tryptophan; D001120:Arginine; D012964:Sodium; D002268:Carboxypeptidases; D008274:Magnesium