In vitro synthesis and repression of argininosuccinase in Escherichia coli K12; partial purification of the arginine repressor.
Phi80dargECBH DNA has been used to direct cell-free synthesis of argininosuccinase, the argH gene product in Escherichia coli K12. In vitro enzyme synthesis is sensitive to repression by partially purified preparations from an argR+ strain but not by corresponding preparations from an argR- strain. Using DNA-cellulose chromatography, approximately seventyfold purification of repressor has been obtained. The partially purified preparation represses argininosuccinase synthesis but has no effect on beta-galactosidase synthesis.
D001120:Arginine / Q000378:metabolism*; D001123:Argininosuccinate Lyase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D004247:DNA / Q000032:analysis; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology*; D005696:Galactosidases / Q000378:metabolism; D005796:Genes; D008190:Lyases / Q000378:metabolism*; D009154:Mutation
D004247:DNA; D001120:Arginine; D005696:Galactosidases; D008190:Lyases; D001123:Argininosuccinate Lyase