Isolation of cells that retain differentiated functions in vitro: properties of clonally isolated type II alveolar pneymonocytes.
We have isolated byclonal culture techniques a diploid cell strain (L-2) from adult rat lung. These cells appear to retain differentiated functions that are present in type II alveolar epithelial cells of intact lung. The L-2 cells are diploid, epithelial cells, they contain osmiophilic lamellar bodies in their cytoplasm and they synthesize lecithin by the same de novo pathways as whole lung.
D000818:Animals; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D002999:Clone Cells; D003584:Cytological Techniques; D005260:Female; D005615:Freezing; D050356:Lipid Metabolism; D008715:Methionine / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D013997:Time Factors
D010713:Phosphatidylcholines; D008715:Methionine; D002794:Choline