Hemoglobin biosynthesis in individual bursts in culture: studies of human umbilical cord blood.
We cultured human umbilical cord blood erythropoietic precursors in methyl cellulose clonal assay and analyzed the synthetic rates of Hb A and Hb F in individual erythropoietic bursts. Hemoglobin was labeled with 14C-amino acids in culture, separated by slab gel isoelectric focusing techniques, and quantitated by fluorographic methods. Almost all bursts exhibited both Hb A and Hb F in varying ratios. Frequencies of the individual bursts differing in percentage Hb F biosynthesis had normal distributions. Natal erythropoietic precursors appeared to be randomly committed to Hb F synthesis.
D002478:Cells, Cultured; D004920:Erythropoiesis; D005312:Fetal Blood / Q000502:physiology*; D005319:Fetal Hemoglobin / Q000096:biosynthesis*; D006441:Hemoglobin A / Q000096:biosynthesis*; D006451:Hemoglobin, Sickle / Q000096:biosynthesis; D006801:Humans
D006451:Hemoglobin, Sickle; D006441:Hemoglobin A; D005319:Fetal Hemoglobin