Demonstration of a specific neutrophil receptor for a cell-derived chemotactic factor.
The crystal-induced chemotactic factor, a cell-derived chemoattractant for neutrophils, binds specifically to a site on human neutrophils but not to erythrocytes or lymphocytes, suggesting a relationship between the presence of specific binding sites on the neutrophils and the ability to be chemotactically activated. The Scatchard analysis revealed an equilibrium dissociation constant at 37 degrees C of 0.446 micrometer and the presence of approximately equal to 6.44 x 10(5) binding sites for 125I-crystal-induced chemotactic factor per cell. Binding was not displaced by the synthetic chemotactic factors F-Met-Leu-Phe and Gly-His-Gly or by complement-activated plasma providing evidence of the specificity of the receptor.
D001665:Binding Sites; D002634:Chemotaxis, Leukocyte; D006023:Glycoproteins / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism
D006023:Glycoproteins