Failure of endotoxin to increase nonspecific resistance to infection of lipopolysaccharide low-responder mice.
In vitro and in vivo responses to lipopolysaccharide (LPS) and various other bacterial immunostimulants were compared in c3H/He low-responder mice. The principal findings were as follows. (i) Their splenic lymphocytes were stimulated by various gram-negative mitogens such as an Escherichia coli peptidoglycan, a detoxified derivative of LPS, and even endotoxins extracted by trichloroacetic acid that are known to contain protein; spleen cells of these mice were also transformed by two other B-cell mitogens extracted from acid-fast organisms. (ii) Their macrophages were refractory to LPS and weakly responsive to a mycobacterial prepartion. (iii) LPS failed to elicit nonspecific resistance in these mice against Klebsiella pneumoniae infection. (iv) Endotoxin extracted by trichloroacetic acid and a mycobacterial preparation that could increase nonspecific resistance to infection in other strains did not protect C3H/He mice against a challenge by K. pneumoniae, although both prepartions could evoke nonspecific responses of B cells in this low-responder subline.
D000818:Animals; D002452:Cell Count; D002470:Cell Survival; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli / Q000276:immunology; D007710:Klebsiella Infections / Q000517:prevention & control*; D007711:Klebsiella pneumoniae; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009161:Mycobacterium / Q000276:immunology; D009615:Nocardia / Q000276:immunology; D010457:Peptidoglycan; D012477:Salmonella enteritidis / Q000276:immunology; D014238:Trichloroacetic Acid
D004731:Endotoxins; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D014238:Trichloroacetic Acid