HLA membrane antigens: sequencing by intrinsic radioactivity.
Radiochemical sequence data are presented for the amino termini of mixed HLA antigens A1 and B8 isolated from a human lymphoblastoid cell line. Cells were labeled intrinsically in small-scale tissue culture with 14C-labeled amino acids and [14C]pyruvate. The specific activities obtained were sufficiently high and uniform to permit direct radiochemical sequencing of the antigens. HLA antigens were isolated by adsorption to a solid-phase anti-beta2-microglobulin immunoadsorbent followed by electrophoresis. The method should be generally applicable and useful for sequencing all parts of the molecule.
D000595:Amino Acid Sequence; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006680:HLA Antigens / Q000032:analysis* / Q000302:isolation & purification; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D007164:Immunosorbents; D008565:Membrane Proteins / Q000032:analysis; D008722:Methods; D001613:beta 2-Microglobulin
D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007164:Immunosorbents; D008565:Membrane Proteins; D001613:beta 2-Microglobulin