A method of embedding tissue culture preparations in situ for transmission electron microscopy.
A method for embedding tissue culture preparations, grown on Millipore filters, in Araldite is described. Accurate light microscopic localization of areas suitable for ultrathin sectioning is possible. The plane of sectioning relative to the Millipore filter can be varied to suit individual experimental needs.
D002478:Cells, Cultured; D003584:Cytological Techniques; D008264:Macrophages; D008854:Microscopy, Electron