Localization of smooth muscle-like contractile proteins in kidney by immunoelectron microscopy.
Contractile proteins of smooth muscle type were found in kidney cells by immunological methods. The reactions of rabbit anti-actin, anti-heavy meromyosin and anti-myosin antisera with rat kidney were investigated by immunoelectron microscopy. Anti-actin stained specifically the foot processes of epithelial cells in the glomerulus, the basal processes of tubular epithelial cells, and the cytoplasm of smooth muscle cells. Anti-heavy meromyosin stained the foot processes at their bases near the cell membrane and close to the basement membrane. Anti-myosin stained the cytoplasm of vascular smooth muscle cells. It is suggested that actin and heavy meromyosin-like proteins may act together to cause movement of foot process cytoplasm.
D000199:Actins / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis; D007668:Kidney / Q000032:analysis* / Q000648:ultrastructure; D007678:Kidney Glomerulus / Q000032:analysis; D009124:Muscle Proteins / Q000032:analysis*; D009130:Muscle, Smooth / Q000032:analysis; D015879:Myosin Subfragments / Q000032:analysis; D009218:Myosins / Q000032:analysis; D051381:Rats
D000199:Actins; D009124:Muscle Proteins; D015879:Myosin Subfragments; D009218:Myosins