Methylated nucleosides in polyadenylate-containing yeast messenger ribonucleic acid.
Yeast messenger RNA was found to be methylated. A calculation of the specific methylation showed that the average yeast messenger RNA molecule contains only two methylated nucleosides which occur in one alkali stable oligonucleotide. Similar to other eukaryotic messengers, the 5' terminus of yeast messenger RNA is blocked by 7-methylguanosine, linked through a di- or triphosphate bridge to a ribosemethylated nucleoside. Contrary to the messengers of high eucaryotic organisms, no additional base methylated constituents were found.
D002499:Centrifugation, Density Gradient; D008745:Methylation; D009843:Oligoribonucleotides / Q000032:analysis; D011061:Poly A / Q000032:analysis*; D011523:Protoplasts / Q000032:analysis; D012333:RNA, Messenger; D012263:Ribonucleosides / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D009843:Oligoribonucleotides; D012333:RNA, Messenger; D012263:Ribonucleosides; D011061:Poly A