An electron microscopic study of pyruvate carboxylase.
Pyruvate carboxylases, purified to homogeneity from sheep and chicken liver, have been examined in the electron microscope and found to have a splayed tetrahedral structure which, in some views, appears similar to that described for this enzyme isolated from yeast. The square-planar tetrameric image previously ascribed to pyruvate carboxylases from vertebrate sources was shown to be that of a molecule which is not only larger than pyruvate carboxylase, but also devoid of biotin.
D000818:Animals; D002645:Chickens; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D011487:Protein Conformation; D011766:Pyruvate Carboxylase / Q000302:isolation & purification; D012756:Sheep; D013045:Species Specificity
D046911:Macromolecular Substances; D011766:Pyruvate Carboxylase