title: The pepsinogens of cat gastric mucosa and the pepsins derived from them.
abstract: Fundic mucosal extracts of the cat were examined by chromatography using DEAE-cellulose at pH 5.3. The peaks of proteolytic activity located by this method were shown to be heterogeneous on electrophoresis. By electrophoresis of individual homogenous chromatographic fractions 9 separate zones of proteolytic activity were detected. One zone did not migrate from the origin and is possibly a non-pepsinogen gastric protease. Simultaneous electrophoresis of pepsinogens and pepsins indicated that each pepsinogen gave rise to one pepsin. One zymogen gives rise to a pepsin active with the synthetic substrate acetyl-L-phenylalanyl-L-diiodotyrosine (APDT).
mesh_terms: D000818:Animals; D002415:Cats; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004587:Electrophoresis, Agar Gel; D005753:Gastric Mucosa / Q000032:analysis*; D010434:Pepsin A / Q000032:analysis*; D010435:Pepsinogens / Q000032:analysis*; D010447:Peptide Hydrolases / Q000032:analysis
chemical_list: D010435:Pepsinogens; D010447:Peptide Hydrolases; D010434:Pepsin A