Physical association of histocompatibility antigens and tumor-associated antigens on the surface of murine lymphoma cells.
Serologic and immunochemical techniques were used to characterize plasma membrane antigens of the murine lymphoma 6C3HED. Syngeneic C3H/HeJ anti-6C3HED antisera were prepared and were shown by cytotoxicity, absorption, and immunoprecipitation tests to be specifically reactive with the lymphoma cells. Alloantisera to H-2.23 were found to be unreactive with intact lymphoma cells by cytotoxicity and absorption assays. However, H-2.23 antigens were detectable in tumor cell lysates by inhibition of cytotoxicity and immunoprecipitation. SDS-PAGE electropherograms of immunoprecipitates of radiolabeled tumor cell extracts obtained with anti-6C3HED and anti-H2.23 antisera were essentially identical; proteins with Mr of approximately 70,000, 45,000, and 12,000 were apparent in both. An immuno-adsorbent of insolubilized anti-6C3HED removed H-2.23 antigens detectable in lymphoma extracts, but had no effect on H-2.23 antigens in normal splenocyte extracts. These data indicate that H-2.23 and TAA are physically associated on 6C3HED cells.
D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D004591:Electrophoresis, Polyacrylamide Gel; D006183:H-2 Antigens; D007106:Immune Sera / Q000494:pharmacology; D007164:Immunosorbents / Q000494:pharmacology; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006183:H-2 Antigens; D007106:Immune Sera; D007164:Immunosorbents