Enzymatic analysis of C27 sterol-accumulating yeast strains.
Several strain of bakers' yeast that accumulate only C27 sterols were analyzed for sterol methyltransferase activity, with no activity being found. Cholesta-5,7,22,24-tetraene-3beta-ol, one of the mutants' sterol products, was found to be an unacceptable substrate for in vitro transmethylation.
D004875:Ergosterol / Q000096:biosynthesis; D008715:Methionine / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis*
D013261:Sterols; D008715:Methionine; D008780:Methyltransferases; D004875:Ergosterol