Affinity chromatography. A new technique for partial purification of human leucocyte migration inhibitory factor.
Human leucocyte migration inhibitory factor (LIF) was subjected to affinity column chromatography on Sepharose-bound alpha-L-fucose. Following the addition of alpha-L-fucose to the running buffer adsorbed lymphokine was eluted almost quantitatively. A minimum of 70-fold purification of LIF was achieved.
D002846:Chromatography, Affinity; D005643:Fucose / Q000494:pharmacology; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008263:Macrophage Migration-Inhibitory Factors / Q000302:isolation & purification*; D012685:Sepharose / Q000494:pharmacology
D008263:Macrophage Migration-Inhibitory Factors; D005643:Fucose; D012685:Sepharose