Plasmodium berghei: characteristics of a selected population of small free blood stage parasites.
The characteristics of a selected population of small blood stage parasites obtained by differential centrifugation of a population of P. berghei parasites freed by continuous flow sonication are described. About 10% of these free parasites are merozoites, many others are transitional forms having some merozoite characteristics. The parasite preparations are infectious and sufficiently resistant to incubation at 37 degrees C to be useful experimentally. Disc gel electrophoresis analysis indicates that these small parasites differ in composition from an unselected intraerythrocytic P. berghei population.
D000818:Animals; D000941:Antigens; D002458:Cell Fractionation; D004912:Erythrocytes / Q000469:parasitology; D008288:Malaria / Q000469:parasitology; D051379:Mice; D010962:Plasmodium berghei / Q000737:chemistry / Q000302:isolation & purification* / Q000472:pathogenicity; D011506:Proteins / Q000032:analysis; D051381:Rats; D013010:Sonication
D000941:Antigens; D011506:Proteins