The maintenance affinities of KLF-1 proximal F merogenotes in Escherichia coli.
In conjugation with donor strains carrying proximal F merogenotes of KLF-1 type about 100-fold lower frequency of Leu+ or Lac+ recombinants was found. The determination of the level of beta-galactosidase synthesis during the initial period of mating indicated that the transfer process of plasmid DNA was not impaired. Among the recombinants selected a large fraction have not expressed the plasmic fertility functions. This phenomenon was found to be replicon specific and was observed only with proximal F merogenotes but not with classical F'lac and F'ORF-1 elements or R1-19 plasmid. The expression of KLF-1 plasmid functions in the cell seems to be affected by a chromosomal gene of the proximal F merogenote closely linked to leu marker.
D000667:Ampicillin / Q000494:pharmacology*; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology; D005111:Extrachromosomal Inheritance; D005696:Galactosidases / Q000096:biosynthesis; D009154:Mutation; D010403:Penicillin Resistance; D010641:Phenotype; D010957:Plasmids; D011995:Recombination, Genetic
D000667:Ampicillin; D005696:Galactosidases