Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent.
A modified 125I-labelled DNA-binding test was developed. By using an anionic detergent, 0-025% sodium dodecyl sulphate, the non-specific binding of DNA by basic proteins was completely eliminated, while the specific binding of DNA by anti-DNA antibodies was not interfered with. The level of DNA-binding activity in normal human sera was decreased in the modified DNA-binding test, while its sensitivity was similar to that of the Farr assay in presence of sera from SLE patients.
D000906:Antibodies / Q000032:analysis*; D001666:Binding Sites, Antibody; D004247:DNA / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D007938:Leukemia / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D011485:Protein Binding / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D000906:Antibodies; D012967:Sodium Dodecyl Sulfate; D004247:DNA