Induction by parathyroid hormone of ornithine decarboxylase in rabbit costal chondrocytes in culture.
The activity of ornithine decarboxylase (ODC) in rabbit costal chondrocytes in culture markedly increased after addition of parathyroid hormone (PTH), reaching a maximum 4 to 5 h after PTH addition. The effect of PTH was dose-dependent both in the presence and absence of fetal calf serum. Addition of cycloheximide or actinomycin D prevented the induction of ODC by PTH. Calcitonin, 1,25-dihydroxyvitamin D3, and vitamin A had no stimulatory effect on the activity of ODC, and did not inhibit the induction of ODC by PTH, when added with PTH.
D000818:Animals; D002116:Calcitonin / Q000494:pharmacology; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004100:Dihydroxycholecalciferols / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D011817:Rabbits
D004100:Dihydroxycholecalciferols; D010281:Parathyroid Hormone; D003609:Dactinomycin; D002116:Calcitonin; D003513:Cycloheximide; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase