A replica plating method for CHO cells using nylon cloth.
A new technique of replica plating for CHO cells has been developed in which the pattern of colonies growing in a tissue culture plate is reproduced on a nylon cloth. The efficiency of replica plating onto the nylon cloth is affected by the colony size and the time of cloth-to-plate contact. Maximum replica plating efficiency (80-90%) occurs using 5- to 6-day-old colonies and a contact time of 4 h. The method easily allows 10(4) colonies to be examined for mutant phenotypes. The effectiveness of the technique is demonstrated by isolation of a clone deficient in activity of glucose-6-phosphate dehydrogenase.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002999:Clone Cells; D006224:Cricetinae; D005260:Female; D005821:Genetic Techniques; D005955:Glucosephosphate Dehydrogenase Deficiency; D009154:Mutation; D009757:Nylons; D010053:Ovary / Q000166:cytology* / Q000201:enzymology
D009757:Nylons