RNA chain elongation on a chromatin template.
The rate of RNA chain elongation has been measured with DNA and chromatin as template. RNA propagation on chromatin is about 50% of the rate found with DNA. Kinetic experiments demonstrate that the inhibition is not due to interference with the addition of the nucleoside triphosphates. Analysis of the dependence of propagation on the Tm of DNA shows that the chromatin proteins interfere with the translocation of the RNA polymerase along the DNA template.
D000818:Animals; D001665:Binding Sites; D002645:Chickens; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli; D006657:Histones / Q000378:metabolism; D007700:Kinetics; D011189:Potassium Chloride / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis*; D012265:Ribonucleotides / Q000494:pharmacology; D013696:Temperature; D013698:Templates, Genetic
D002843:Chromatin; D004269:DNA, Bacterial; D006657:Histones; D012265:Ribonucleotides; D012313:RNA; D011189:Potassium Chloride; D004247:DNA