title: alpha-Ketoglutarate dehydrogenase mutant of Rhizobium meliloti.
abstract: A mutant of Rhizobium meliloti selected as unable to grow on L-arabinose also failed to grow on acetate or pyruvate. It grew, but slower than the parental strain, on many other carbon sources. Assay showed it to lack alpha-ketoglutarate dehydrogenase (kgd) activity, and revertants of normal growth phenotype contained the activity again. Other enzymes of the tricarboxylic acid cycle and of the glyoxylate cycle were present in both mutant and parent strains. Enzymes of pyruvate metabolism were also assayed. L-Arabinose degradation in R. meliloti was found to differ from the known pathway in R. japonicum, since the former strain lacked 2-keto-o-deoxy-L-arabonate aldolase but contained alpha-ketoglutarate semialdehyde dehydrogenase; thus, it is likely that R. meliloti has the L-arabinose pathway leading to alpha-ketoglutarate rather than the one to glycolaldehyde and pyruvate. This finding accounts for the L-arabinose negativity of the mutant. Resting cells of the mutant were able to metabolize the three substrates which did not allow growth.
mesh_terms: D000085:Acetates / Q000378:metabolism; D001089:Arabinose / Q000378:metabolism*; D002952:Citric Acid Cycle; D006038:Glyoxylates / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism*; D007658:Ketone Oxidoreductases / Q000378:metabolism*; D009154:Mutation; D011773:Pyruvates / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics / Q000378:metabolism*
chemical_list: D000085:Acetates; D006038:Glyoxylates; D011773:Pyruvates; D001089:Arabinose; D007658:Ketone Oxidoreductases; D007655:Ketoglutarate Dehydrogenase Complex