Visualization principles in thin-layer immunoassays (TIA) on plastic surfaces.
Macromolecules may adsorb firmly as a monolayer to plastic surfaces and still retain their property to react specifically with antibodies. In the present communication four different principles for visualization of such antigenantibody interactions on plastic surfaces are described. In addition, a diffusion-ingel method for quantitation of antibodies is presented. The described methods offer simple, sensitive and accurate means of assaying antigen-antibody reactions.
D000818:Animals; D000937:Antigen-Antibody Reactions; D006041:Goats; D006382:Hemadsorption; D007106:Immune Sera / Q000494:pharmacology; D007118:Immunoassay / Q000379:methods*; D007163:Immunosorbent Techniques; D010969:Plastics; D011817:Rabbits; D014867:Water / Q000378:metabolism
D007106:Immune Sera; D010969:Plastics; D014867:Water