Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid.
pML31, a plasmid chimera constructed to contain the replication genes of an Flac plasmid, has been studied by electron microscope methods. Heteroduplex analysis shows that the only F sequence present in pML31 is that with corrdinates 40.3-49.3F. This region has previously been identified as essential for plasmid maintenance. The sequence of pML31, which was derived originally from R6-5, carries the km gene(s) and an inverted duplication of a 1.0-kilobase sequence. On the basis of length measurements, the repeated sequence is different from IS1, IS2, IS3, and an inverted repeat associated with the km gene(s) of plasmid JR67.
D001483:Base Sequence; D003227:Conjugation, Genetic; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis; D004270:DNA, Circular / Q000032:analysis; D004277:DNA, Single-Stranded / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis* / Q000378:metabolism; D005144:F Factor; D005796:Genes; D008854:Microscopy, Electron; D008961:Models, Structural
D004269:DNA, Bacterial; D004270:DNA, Circular; D004277:DNA, Single-Stranded