Location of a high-sulphur protein in developing wool follicles using peroxidase-labelled antibody.
Antiserum to a high-sulphur protein (SCMK-B2) from wool was purified to obtain the immunoglobin G fraction, and this was labelled with horse-radish peroxidase. The labelled antibody was then applied to sections of sheep skin embedded in glycol methacrylate in order to locate the distribution of high-sulphur protein within the lower hair shaft. Regions containing the highest concentration of high-sulphur protein were the cuticle of the hair shaft and the keratogenous zone of the cortex.
D000818:Animals; D000918:Antibody Specificity; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D011506:Proteins / Q000032:analysis*; D012756:Sheep / Q000378:metabolism*; D012867:Skin / Q000032:analysis; D014935:Wool / Q000032:analysis*
D011506:Proteins; D006735:Horseradish Peroxidase