title: Location of the Aspartase Gene (aspA) on the linkage map of Escherichia coli K12.
abstract: The aspartase (L-asparatate ammonia-lyase, EC. 4.3.I.I) structural gene, aspA, was mapped by two-factor and three-factor transductional crosses using phage PI. Cotransduction frequencies between aspA and other markers were: ampA (69%); frdA (48 TO 67%) mel (35%); purA (17%); fdp (I-6%). The sequence of markers within the corresponding segment (91 to 95 min) of the Escherichia coli linkage map was shown to be mel-aspA-ampA-frdA-purA-fdp.
mesh_terms: D000642:Ammonia-Lyases / Q000096:biosynthesis*; D001220:Aspartate Ammonia-Lyase / Q000096:biosynthesis*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005796:Genes; D008040:Genetic Linkage; D005971:Glutamates / Q000378:metabolism; D009154:Mutation; D014161:Transduction, Genetic
chemical_list: D005971:Glutamates; D000642:Ammonia-Lyases; D001220:Aspartate Ammonia-Lyase