A solid phase radioimmunoassay for pancreatic glucagon.
A solid phase radioimmunoassay procedure has been applied for the determination of glucagon and some variables of the assay procedure were studied. The sensitivity of the assay was 10 pg of glucagon for samples assayed in triplicate. This assay method which has a high precision and is technically simple as compared with other radioimmunoassays, was found to be suitable for studies on glucagon release in vitro.
D000818:Animals; D000917:Antibody Formation; D001666:Binding Sites, Antibody; D002210:Caprylates / Q000494:pharmacology; D005934:Glucagon / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera; D007515:Islets of Langerhans / Q000378:metabolism; D010179:Pancreas / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D002210:Caprylates; D007106:Immune Sera; D005934:Glucagon