Activation of long chain fatty acids with acyl carrier protein: demonstration of a new enzyme, acyl-acyl carrier protein synthetase, in Escherichia coli.
A soluble enzyme activity which catalyzes the synthesis of acyl-acyl carrier protein from acyl carrier proteins, a long chain fatty acid, and ATP has been demonstrated in E. coli. The reaction requires high concentrations of both Ca++ and Mg++ for activity, and cleaves ATP to AMP and PPi. The fatty acyl product has been identified as acyl-acyl carrier protein by its solubility, thioester linkage, molecular weight, charge, and biological activity. Several criteria indicate the enzyme is distinct from acyl-CoA synthetase. The fatty acid specificity of the enzyme suggests a role of acyl-acyl carrier protein synthetase in the incorporation of fatty acids into phospholipid.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D002118:Calcium; D002352:Carrier Proteins; D003597:Cytosine Nucleotides / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology*; D005227:Fatty Acids / Q000378:metabolism; D005796:Genes; D007700:Kinetics; D008025:Ligases / Q000378:metabolism*; D008274:Magnesium; D010168:Palmitates / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis; D013438:Sulfhydryl Compounds / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003597:Cytosine Nucleotides; D005227:Fatty Acids; D010168:Palmitates; D010743:Phospholipids; D013438:Sulfhydryl Compounds; D000255:Adenosine Triphosphate; D008025:Ligases; D008274:Magnesium; D002118:Calcium