[A change in the activity of the beta-phenylpropionic acid conversion enzyme in Escherichia coli under the influence of transmissive plasmids of Salmonella heidelberg].
The activity of the enzyme of the conversion of beta-phenylpropionic acid was studied in the strains of Escherichia coli serotypes 055:K59:H2 and O111:K58:H2 used as recipients via transmission of R- and Col-plasmids by conjugation in vitro. The activity of this enzyme was determined by modified method of Ben Hamida [3]. The wild type strain of Salmonella heidelberg carrying ColIb plasmid and preliminary obtained R1-19 plasmid from E. coli J 5-3 was used as a donor. The activity of the enzyme of conversion of beta-phenylpropionic acid in recombinants carrying R1-19ColIb plasmids was 3-5 times lower as compared with the original recipient. The colour reaction drived under the growth of original bacterial strains in the nutrient broth agar contained beta-phenylpropionic acid at the final concentration of 20 mg% was completely inhibited with that recombinants. The activity of this enzyme in recombinants carrying only R1-19 or ColIb plasmid remains unchanged.
D003227:Conjugation, Genetic; D004794:Enzyme Repression; D004798:Enzymes / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D010666:Phenylpropionates; D010957:Plasmids; D011995:Recombination, Genetic; D012475:Salmonella
D004798:Enzymes; D010666:Phenylpropionates
Izmenenie aktivnosti fermenta konversii beta-fenilpropionovoĭ kisloty shtammov Escherichia coli pod deĭstviem transmissivnykh plazmid Salmonella heidelberg