title: Selective breeding of mice for antibody responsiveness to flagellar and somatic antigens of salmonellae.
abstract: Selective breeding has been performed in mice for their high or low antibody responsiveness to Salmonella antigens (Ag). Two noncross-reacting Salmonellae (S. typhimurium and S. oranienburg) have been alternately used to immunize successive generations in order to avoid interference of maternally transmitted antibody. Both Salmonellae carry two independent antigens: flagellar (f) and somatic (s). Two two-way selections were carried out: one for agglutinin response to fAg and the other for agglutinin response to sAg (Selection Ags). The agglutinin response to the other independent Ag was also measured (Associated Ags). The phenotypic character chosen for selection is the maximal agglutinin titer in response to optimal immunization. In both selections, high and low responder lines diverged progressively. There was a 27-fold interline difference in the F13 generation of selection for fAg response and a 38-fold one in the F11 generation of selection for sAg response. This divergence and the continuous distribution of phenotypes in the foundation population indicate that the quantitative character investigated is subject to polygenic regulation. The realized heritability (h2) of the character, measured from the interline divergence, was 0.18 +/- 0.06 in both f and s selections. This means that about 20% of the phenotypic variance of the foundation population is additive. The modification of immune responsiveness to the selection Ag produced by selective breeding was accompanied by a parallel and equivalent effect on immune responsiveness to the noncross-reacting Associated Ag. This effect is therefore entirely nonspecific. A small but definite sex effect in favor of female antibody responsiveness was demonstrated. The results are discussed in comparison with other selection experiments made in mice for quantitative antibody response to other antigens.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial; D004306:Dose-Response Relationship, Immunologic; D005407:Flagella / Q000276:immunology; D005796:Genes; D051379:Mice; D012475:Salmonella / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology; D012737:Sex Factors
chemical_list: D000942:Antigens, Bacterial