title: [Study of the methods for detecting Salmonella in meat and viscera].
abstract: Studied were a toal 415 samples of viscera (musculature, liver, spleen, kidneys and a lymph node from skeletal musculature) taken from 83 emergency slaughtered pigs and cattle, and liver samples of 10 experimentally infected guinea pigs using direct immunofluorescent microscopy. All samples were preliminary kept in the tetrathionate-broth of Müller and Kaufmann, the broth for cultivation of Gramnegative bacteria, and in paraffin blocks, for 24 hours, in order to obtain more abundant growth of bacteria. The cultures were studied by the standard method of reseeding from liquid nutrient media at the 24th, 48th and 72nd hour. Highest per cent of correlation (89.4%) between the results of the immunofluorescence method and the cultural method was shown by enriching the growth in the tetrathionate-broth of Müller and Kaufmann. This suggests the use of immunofluorescence microscopy as a quick helpful method to detect Salmonella bacteria in the microbiologic inspection of meat.
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D002031:Bulgaria; D002417:Cattle; D005455:Fluorescent Antibody Technique; D005516:Food Microbiology; D006168:Guinea Pigs; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008460:Meat / Q000032:analysis*; D012475:Salmonella / Q000302:isolation & purification*; D013154:Spleen / Q000382:microbiology; D013552:Swine
vernacular_title: Prouchvane na metodite za otkrivane na salmoneli v meso i vutreshni organi