Direct implantation of Ph1 chromosome-positive myeloblasts into newborn hamsters.
Peripheral leukocytes from a male patient with chronic myelogenous leukemia in blast crisis were implanted directly into the intraperitoneal cavity of seven newborn hamsters treated with antilymphocyte serum. At sacrifice 17-19 days after implantation, three hamsters were found to have disseminated tumors. The other four hamsters were cannibalized. Chromosome analysis of cells from the enlarged lymph nodes revealed the presence of a human male karyotype. Most metaphase cells were pseudodiploid with the Ph1 chromosome and isochromosome 17, while a few metaphase cells were classically diploid. Identical chromosome constitutions were observed in the original peripheral leukocytes of the patient. The results strongly suggested simultaneous heterotransplantation of Ph1-positive leukemic and normal leukocytes.
D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002875:Chromosomes; D002904:Chromosomes, Human, 21-22 and Y; D006224:Cricetinae; D006801:Humans; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics; D008297:Male; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous