Diazo replication of the lung: an improved methodology.
The methodology of diazo replication has been improved to provide replicas of 60 mum-thick lung sections with high contrast and sharp delineation of airways and air spaces. The newly introduced techniques are critical temperature control, embedment in isopentane, Bismarck brown staining, and parallel light, halogen lamp illumination.
D006652:Histological Techniques; D008168:Lung / Q000033:anatomy & histology*; D008722:Methods; D010781:Photography / Q000379:methods*