title: Scanning electron microscopy of postoperative M-K and moist chamber-stored corneas.
abstract: In the present study, using the in vivo test situation which all corneal donor material must meet, we performed penetrating keratoplastics utilizing corneal tissue stored for five days in either M-K medium or conventional moist chambers. Cat corneas stored for five days in M-K medium had a higher incidence of postoperative graft clarity along with an intact endothelial cell layer as determined by scanning electron microscopy than corneas stored in a conventional moist chamber. The endothelium of corneas immediately after moist chamber storage revealed minimal surface alteration which contrasted to the more severely stressed endothelium immediately after M-K storage. However, the M-K stored endothelium survived the trauma of penetrating keratoplasty much better than cells stored in moist chambers.
mesh_terms: D000818:Animals; D002415:Cats; D003315:Cornea / Q000648:ultrastructure; D016039:Corneal Transplantation; D004727:Endothelium / Q000648:ultrastructure; D012996:Solutions; D014021:Tissue Preservation / Q000379:methods*; D014184:Transplantation, Homologous
chemical_list: D012996:Solutions