[Use of rat glomerular basement membrane (GBM) as antigen in indirect imunofluorescence test for detection of human anti-GBM antibodies (author's transl)].
The purpose of this paper is to verify if we may use the rat glomerular basement membrane (GBM) as antigen, in indirect immunofluorescent test in isolated GBM (IIT-BGM) to detect human anti GBM antibodies. Rabbit anti-human GBM serum (A-H-GBMS) were obtained by rabbit's immunization with human GBM or rat GBM respectively. The content of anti-GBM antibodies in rabbit's sera was determined in IIT-GBM. The titer of the A-H-GBMS was 1/10 when determined in IIT-GBM performed in human GBM and only weakly positive in IIT-GBM performed in rat GBM. The titer of A-R-GBMS was 1/60 and 1/20 when determined respectively in rat GBM and in human GBM. All the reactions performed with sera absorbed with autologous GBM were negative; the absorption of sera with heterologous GBM reduced sera titer.


CONCLUSIONS
a) rat and human GBM have common and different antigenic components; b) to detect human anti-GBM antibodies we must not employ rat GBM in IIT-GBM.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D000941:Antigens; D001485:Basement Membrane / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology*; D051381:Rats
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens
Emprego de membrana basal glomerular (MGB) de rato em reações de imunofluorescência indireta para pesquisa de anticorpo anti-membrana basal glomerular humana