Soluble HL-A antigens in serum. I. Isolation and purification.
A new method was developed which utilizes fractional salting out, gel filtration, ion exchange chromatography and polyacrylamide gel electrophoresis for the isolation and purification of soluble HL-A antigens from serum. The method produced purified antigens possessing HL-A 5,9 specificities with yield up to 60% of that detected in the original serum. Up to 150-fold increase in specific HL-A antigenic activity above that in the starting material could be achieved as measured by the ability of the purified antigens to specifically inhibit the cytotoxic activity of HL-A alloantisera against selected target cells. Electrophoretically purified HL-A antigens with different specificities appear to have a similar molecular size, i.e. 33 000, but differ in their electric charge properties, beta2-microglobulin (beta2m) seems to be noncovalently associated with HL-A antigens present in serum, but during purification beta2m in progressively lost until at the final purification by polyacrylamide gel electrophoresis, the material is completely devoid of beta2m.
D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006680:HLA Antigens / Q000302:isolation & purification*; D006649:Histocompatibility Antigens / Q000302:isolation & purification*; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight; D001613:beta 2-Microglobulin / Q000302:isolation & purification
D006680:HLA Antigens; D006649:Histocompatibility Antigens; D001613:beta 2-Microglobulin