title: Electrophoretic mobility of human lymphocytes purified by nylon wool columns and spontaneous sheep red blood cells rosetting techniques correlation with immunofluorescence.
abstract: Cell electrophoresis allows separation of normal human lymphocytes into two principal groups which are a function of their relative rates of migration. In 42 healthy adults, 19.9% of the lymphocytes have a slower migration rate, and 80.1%, a faster migration rate than the reference speed (1 mum - sec-1 - cm). Two methods are used for the selection of the lymphocytic populations: spontaneous rosetting with sheeps red blood cells, a property of T-lymphocytes, and adherence to nylon wool columns, which preferentially selects B-lymphocytes. The cells which do not form spontaneous rosettes, but adhere to nylon wool columns show mainly slow migration. Cells which do not adhere to nylon columns show a faster migration rate. These findings affirm the T-nature of the rapidly migrating lymphocytes, and the B-nature of the slow-migrating lymphocytes. Results by the immunofluorescence technique confirm this.
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D002469:Cell Separation; D004586:Electrophoresis; D004903:Erythrocyte Aggregation; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007104:Immune Adherence Reaction; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009757:Nylons; D012756:Sheep / Q000276:immunology; D013997:Time Factors; D014935:Wool
chemical_list: D009757:Nylons