title: The substrate constant for the ammonium ion of growing Saccharomyces cerevisiae.
abstract: The relation between ammonium concentration and growth rate was studied in steady state continuous cultures of Saccharomyces cerevisiae in nitrogen-limited glucose ammonium medium. This relation could be described by the Monod equation. A maximum specific growth rate of 0.41 h-1 and a substrate constant for ammonium of 5-11 muM were calculated. Ammonium was determined by a modification of the phenol hypochlorite method. A discussion of the results in view of literature data on the substrate constants for other nutrients is given.
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007202:Indicators and Reagents; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism
chemical_list: D007202:Indicators and Reagents; D000641:Ammonia; D005947:Glucose