Polyclonal activation of bone-marrow-derived lymphocytes from human peripheral blood measured by a direct plaque-forming cell assay.
A culture and assay system for the stimulation of human peripheral blood lymphocytes with polyclonal activators of bone-marrow-derived lymphocytes (B cells), such as pokeweed mitogen and Escherichia coli lipopolysaccharide, and subsequent measurement of single cell antibody production by a hemolysis-in-bel direct plaque-forming cell assay against sheep erythrocytes has been established. The critical culture requirements have been delineated and a new highly sensitive ultrathin gel assay method has been described. Under these conditions a substantial and highly reproducible plaque-forming cell response was detected in normal human peripheral blood. This system can be readily used to explore the complex events associated with activation of human B cells.
D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D001769:Blood; D003470:Culture Media; D004926:Escherichia coli; D006462:Hemolytic Plaque Technique; D006801:Humans; D037102:Lectins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D013997:Time Factors
D003470:Culture Media; D037102:Lectins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial