Cloned synthetic lac operator DNA is biologically active.
A chemically synthesised duplex DNA fragment containing the sequence of the lac operator was cloned in E. coli using the vehicle pMB9. Clones containing lac-pMB9 hybrid DNA produced beta-galactosidase constitutively and the hybrid DNA bound the lac repressor specifically.
D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000138:chemical synthesis / Q000378:metabolism; D004274:DNA, Recombinant; D004794:Enzyme Repression; D004926:Escherichia coli; D005696:Galactosidases / Q000096:biosynthesis; D005809:Genes, Regulator; D005818:Genetic Engineering; D007785:Lactose / Q000378:metabolism*; D009876:Operon; D010957:Plasmids; D011485:Protein Binding; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D005696:Galactosidases; D007785:Lactose