Effect of nitrofurazone on bacterial RNA and ribosome synthesis and on the function of ribosomes.
Exposure of E. coli B/r to nitrofurazone strongly inhibits the synthesis of all classes of RNA and both ribosomal sub-units. Polysome formation is likewise inhibited. However, in E. coli nfr-207 a mutant of B/r which lacks nitrofurazone-reductase I, the synthesis of RNA, ribosomal sub-units and formation of polysomes are not significantly affected. This result implies that a reduced metabolite of the drug rather than the drug itself is the active agent. The ability of ribosomes isolated from nitrofurazone-treated E. coli B/r to carry out poly-U directed polyphenylalanine synthesis was lower than that of ribosomes from untreated cells. 14C from labelled nitrofurazone was found to bind to ribosomal sub-units.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D009154:Mutation; D009583:Nitrofurazone / Q000494:pharmacology*; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012270:Ribosomes / Q000187:drug effects / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects*
D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium; D009583:Nitrofurazone