Surfactant ion-pair high-performance liquid chromatography of tryptophan and some of its metabolites in biological fluids.
A rapid, sensitive assay for tryptophan and some of its metabolites in urine, plasma and saliva has been developed using sodium dodecylsulphate as a pairing ion in a surfactant ion-pair high-performance liquid chromatography technique. The method is highly selective for tryptophan which is separated from its main indoleamine metabolites, 5-hydroxytryptophan, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid, and from kynurenine. The usefulness of the assay has been demonstrated in plasma level and urinary excretion studies of orally administered tryptophan.
D001682:Biological Availability; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008297:Male; D008722:Methods; D012463:Saliva / Q000032:analysis; D014364:Tryptophan / Q000032:analysis* / Q000097:blood / Q000652:urine
D014364:Tryptophan