Role of lipopolysaccharide in adsorption of coliphage T4D to Escherichia coli B.
Coliphage T4D was strongly adsorbed to intact lipopolysaccharides and alkaline and lipase-treated lipopolysaccharides from cells of Escherichia coli B, but was not so adsorbed to heat-treated cells. In contrast, coliphage T2h was not adsorbed to lipopolysaccharides and the heat-treated cells. Acid hydrolysate of lipopolysaccharides strongly inhibited the adsorption of phage T4D to acetone and ether-treated cells. The adsorption of phage T4D to the acetone and ether-treated cells was markedly inhibited by authentic D-glucosamine, N-acetyl-D-glucosamine, alpha-methyl-N-acetyl-D-glucosaminide, alpha-methyl-D-glucoside, and D-maltose. Authentic D-glucose and E,L-2,6-diaminopimelic acid also showed similar activity. These compounds did not affect the adsorption of phage T2h to the acetone- and ether-treated cells. Concanavalin A and wheat-germ agglutinin inhibited phage T4D adsorption to the acetone and ether-treated cells probably by blocking the phage-receptor sites on the cell wall. The blocking by concanavalin A and by wheat-germ agglutinin was reversed by alpha-methyl-D-glucoside and by alpha-methyl-N-acetyl-D-glucosaminide, respectively. Results suggested the possibility that coliphage T4D requires N-acetyl-D-glucosaminyl-glucose or glucosyl-D-glucosamine residues of the core of lipopolysaccharides for the initial attachment to the cell wall of Escherichia coli B.
D000096:Acetone / Q000494:pharmacology; D000327:Adsorption; D000596:Amino Acids / Q000494:pharmacology; D001665:Binding Sites; D002241:Carbohydrates / Q000494:pharmacology; D003090:Coliphages / Q000254:growth & development; D003208:Concanavalin A / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005019:Ethyl Ethers / Q000494:pharmacology; D006358:Hot Temperature; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000378:metabolism; D037121:Plant Lectins; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D014908:Triticum / Q000276:immunology
D000596:Amino Acids; D002241:Carbohydrates; D005019:Ethyl Ethers; D037102:Lectins; D008070:Lipopolysaccharides; D037121:Plant Lectins; D011135:Polysaccharides, Bacterial; D003208:Concanavalin A; D000096:Acetone