[Fragmentation of human adenovirus type 6 DNA by means of restrictase of E. co R I and BAM I].
The effect of restricting endonucleases of E. co RI and BAMI on DNA of human adenovirus type 6 was studied. E. co RI and BAMI restrictases cleave virus DNA into 4 fragments each. The molecular weight of E. co RI fragments was: A--17.5X10(6), B--3.63X10(6), C--1.56X10(6), D--1.05X10(6), those of BAMI fragments: A--9.7X10(6), B--7X10(6), C--4.09X10(6), D--3.1X10(6). By means of terminal nucleotidyltransferase and the procedure of partial DNA hydrolysis, the alteration of fragments obtained by the effect of E. co RI enzyme in adenovirus type 6 genome was determined and found to be A--D--C--B.
D000260:Adenoviruses, Human / Q000032:analysis* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D008722:Methods; D008970:Molecular Weight
D004279:DNA, Viral; D004262:DNA Restriction Enzymes
Fragmentatsiia DNK adenovirusa cheloveka tipa 6 s pomoshch'iu restriktaz E. co R I i BAM I