Efficient suppression of the requirement for N function of bacteriophage lambda by a Rho-defective E.coli suA mutant.
The E. coli suA mutant (T82), which is a suppressor of polarity by virtue of its impaired transcription termination factor (rho) activity, is shown to be an efficient suppressor of lambdaN- mutants. The relief of the N requirement by this host is reflected in a substantial restoration of growth and N-dependent beta-galactosidase expression as well as in a partial relief of polarity of N-defective phages.
D003090:Coliphages / Q000201:enzymology / Q000254:growth & development*; D004926:Escherichia coli; D005696:Galactosidases / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D012234:Rho Factor; D013489:Suppression, Genetic; D014157:Transcription Factors; D014158:Transcription, Genetic; D014764:Viral Proteins
D012234:Rho Factor; D014157:Transcription Factors; D014764:Viral Proteins; D005696:Galactosidases