[Utilization of yeast mutants with a defect in the cellular membrane in the search for anticancer antibiotics].
The mutants of Saccharomyces cerevisiae, strain FL 599-I B with defect cell membranes are selectively inhibited by anticancer antibiotics with different molecular mechanisms of action on DNA. At the same time they are insensitive to antibacterial antibiotics. Such mutants may be used as test-cultures in isolation of active agents with antitumor effect from complex mixtures of natural substances. The above test-object makes it easier to perform separation of antimour antibiotics from accompanying bacterial inhibitors.
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects*; D004247:DNA / Q000037:antagonists & inhibitors; D004353:Drug Evaluation, Preclinical; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D015003:Yeasts / Q000187:drug effects*
D000903:Antibiotics, Antineoplastic; D004247:DNA
Ispol'zovanie mutantov drozhzheĭ s defektom kletochnoĭ membrany pri izyskanii protivorakovykh antibiotikov