Repeating restriction fragments of human DNA.
Human DNA digested with Hae III showed multiple repeats of a 170 base pair fragment. The most prominent band was the 340 base pair dimer, estimated to be 0.8% of the entire genome. Eco R1 and Hha I yielded fragments with similar electrophoretic mobility to the Hae III dimer. In each case this band was markedly enriched in DNA reassociating at a 0t of less than or equal to 1. Hybridization of the Hae III dimer to gels eluted on to filters demonstrated that the multiple Hae III fragments and Eco R1 fragments contained compatible sequences. These sequences may comprise a distinct subclass of DNA.
D000328:Adult; D001483:Base Sequence; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000032:analysis; D004247:DNA; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite; D004622:Embryo, Mammalian; D004926:Escherichia coli / Q000201:enzymology; D005260:Female; D006193:Haemophilus influenzae / Q000201:enzymology; D006801:Humans; D008297:Male; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D010920:Placenta / Q000032:analysis; D011247:Pregnancy
D004276:DNA, Satellite; D004247:DNA; D004262:DNA Restriction Enzymes