title: Qualitative and quantitative determination of metapramine and its metabolites in biological materials.
abstract: The qualitative and quantitative determination of metapramine and its metabolites, after selective solvent extraction from biological materials, are achieved by gas-liquid chromatography with or without an internal standard, carried out directly or after derivatization, spectrofluorimetry and one- or two-dimensional thin-layer chromatography. The gas chromatographic procedures are the most sensitive for the quantitative determination fo the unchanged compound: 15 and 35 ng/ml in plasma and 5 and 10 ng/ml in urine; the detection limit for thin-layer chromatography is about 100 ng. These techniques can be used for pharmacokinetic studies and therapeutic controls, and also in analytical toxicology.
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003984:Dibenzazepines / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D008722:Methods; D011619:Psychotropic Drugs / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D003984:Dibenzazepines; D011619:Psychotropic Drugs