Immunofluorescence test for IgM rubella antibodies in whole serum after absorption with anti-gammaFc.
A technique for detecting IgM rubella antibodies by immunofluorescence was developed. Total serum IgG was removed by prior immunoabsorption with anti-gammaFc. This prevents interference by rheumatoid factors in the serum. The test appeared sensitive and specific in sera of rubella convalescents and infants with congenital rubella.
D000042:Absorption; D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M / Q000032:analysis*; D007223:Infant; D012217:Rheumatoid Factor; D012409:Rubella / Q000151:congenital / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor