title: Detergent-containing glucose oxidase reagent for use with the Beckman glucose analyzer.
abstract: We describe a modified glucose oxidase reagent for use in the polarographic determination of glucose with the Beckman "Glucose Analyzer." The reagent contains 1 mL/L of a surfactant (Brij-35 250 g/L solution) as the wetting agent instead of glycerol. Precipitation of components associated with the formulation recommended by Fischl et al. [Clin. Chem. 21, 760 (1975)] does not occur with this reagent. It can be used immediately after preparation. When compared to analytical performance of the commercially prepared reagent, the precision was unchanged by the modified reagent, but the upper limit of accurate response was diminished (7.5 g/L vs. 6.7 g/L). The modified reagent is less expensive than are commercially prepared reagents.
mesh_terms: D001322:Autoanalysis; D001786:Blood Glucose / Q000032:analysis*; D003902:Detergents; D005949:Glucose Oxidase; D006801:Humans; D011048:Polarography / Q000379:methods
chemical_list: D001786:Blood Glucose; D003902:Detergents; D005949:Glucose Oxidase