[Evidence of donoroganspecific humoral antibodies in hyperacutely and acutely rejected canine lungallotransplants (author's transl)].
A method to eluat donororganspecific antibodies enabling direct and specific access to the pattern of humoral rejection after lung allotransplantation is demonstrated. The antibodies were evaluated quantitatively and qualitatively by elution of lung-grafts immunoglobulins and by a consequent investigation of the eluates using the passive haemagglutination- and indirect immunflourescence- test against donors' lung-antigen. Humoral antidonorlung-antibodies could be proved in all rejected grafts belonging to sensitized as well as to unsensitized recipients. There can be seen a highly significant correlation when comparing the results of the passive haemagglutination and indirect immunfluorescence test (r = 0,93, p less than or equal 0,01). On the other hand negative results of the investigations of eluates by lungantigens of other dogs show the specifity of humoral graft rejection. The suprising fact that there does not exist an essential difference between the results of sensitized and nonsensitized animals reveals a humoral immunresponse in hyperacute as well as in acute rejection.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D004285:Dogs; D005455:Fluorescent Antibody Technique; D006084:Graft Rejection; D006386:Hemagglutination Tests; D008168:Lung / Q000276:immunology; D016040:Lung Transplantation; D009928:Organ Specificity; D014184:Transplantation, Homologous
D000906:Antibodies
Nachweis der spenderorganspezifischen humoralen Antikörper in hyperakut und akut abgestossenen Lungenallotransplantaten am Hund