title: A simple method for maintaining fastidious organisms.
abstract: Many strains of nutritionally fastidiuos organisms and anaerobes have been maintained in glycerol broth at-50 C for at least two years without loss of viability. This method of preservation offers convenience and reduces cost.
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media; D005990:Glycerol; D013696:Temperature
chemical_list: D003470:Culture Media; D005990:Glycerol