A method for studying plasma transport of vitamin D applicable to hypervitaminosis D.
In man, vitamin D is normally transported on a specific binding globulin (DBP) and on lipoproteins. In addition, binding to albumin occurs in the presence of vitamin D excess. Agarose gel electrohoresis was used to study the binding of radioactive vitamin D to plasma proteins in lipoprotein-free plasma (d greater than 1.21 g/ml). This method completely separates DBP from albumin and thus enables the quantification of vitamin D bound to these proteins in various clinical and experimental conditions. The same method can be used to study the transport of other vitamin D metabolites.
D001692:Biological Transport; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000097:blood; D004587:Electrophoresis, Agar Gel; D006801:Humans; D006887:Hydroxycholecalciferols / Q000097:blood; D008297:Male; D008722:Methods; D012712:Serum Globulins / Q000378:metabolism; D014807:Vitamin D / Q000097:blood* / Q000506:poisoning
D001798:Blood Proteins; D002352:Carrier Proteins; D006887:Hydroxycholecalciferols; D012712:Serum Globulins; D014807:Vitamin D