[A simple method for evaluation of the lecithin/sphingomyelin ratio in amniotic fluid. Variation in normal and abnormal pregnancies (138 cases) (author's transl)].
The determination of the lecithin/sphingomyelin ratio in amniotic fluid is a useful index for assessing fetal pulmonary maturity. The authors propose a simple and specific method : the amniotic lipids are extracted and thin layer chromatography is carried out in an acetic acid containing medium. The L/S ratio was studied and discussed in 138 normal and pathological cases.
D000653:Amniotic Fluid / Q000032:analysis*; D002855:Chromatography, Thin Layer; D003720:Densitometry; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis; D005333:Fetus / Q000502:physiology; D006801:Humans; D006819:Hyaline Membrane Disease / Q000517:prevention & control; D007202:Indicators and Reagents; D007231:Infant, Newborn; D007455:Iodine; D008055:Lipids / Q000302:isolation & purification; D008168:Lung / Q000254:growth & development; D010713:Phosphatidylcholines / Q000032:analysis*; D010743:Phospholipids / Q000302:isolation & purification; D010758:Phosphorus / Q000032:analysis; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D011296:Prenatal Diagnosis; D013109:Sphingomyelins / Q000032:analysis*
D005231:Fatty Acids, Unsaturated; D007202:Indicators and Reagents; D008055:Lipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins; D010758:Phosphorus; D007455:Iodine
Evaluation simple du rapport lécithines/sphingomyélines du liquide amniotique. Variations au cours des grossesses normales et pathologiques (a propos de 138 cas)