The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane.
A new procedure is established for the analysis of alkaline phosphatase isoenzymes. The electrophoretic separation on cellulose acetate membrane coupled with the detection of alkaline phosphatase activity with 4-methyl-umbelliferyl phosphate as a substrate is described. The proposed method would be useful for the analysis of sample of micro-scale quantities and low activities.
D000469:Alkaline Phosphatase / Q000097:blood*; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D006923:Hymecromone; D007527:Isoenzymes / Q000097:blood*; D013050:Spectrometry, Fluorescence
D007527:Isoenzymes; D006923:Hymecromone; D000469:Alkaline Phosphatase