Immobilized enzymes in continuous-flow analysis.
Glucose oxidase, uricase, and urease were immobilized on the interior surface of activated polyamide tubing. The shelf-life of such enzyme bearing tubes was at least six months. The tubes were used for continuous-flow analysis of glucose, uric acid, and urea with conventional systems and with hybrid micro-scale systems in which modules of different manufacture were combined. The length of enzyme-bearing tube required for each system was ascertained empirically. Each tube could be used for several thousand assays, but glucose oxidase-bearing tubes were more stable than urease- or uricase-bearing tubes. Results for patients' samples correlated well with results obtained by accepted methods.
D001322:Autoanalysis; D001786:Blood Glucose / Q000032:analysis*; D004355:Drug Stability; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D006801:Humans; D008722:Methods; D008832:Microchemistry; D014503:Urate Oxidase; D014508:Urea / Q000032:analysis*
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D014508:Urea; D005949:Glucose Oxidase; D014503:Urate Oxidase