[Use of the antiglobulin test for the purpose of detecting antibodies to enteropathogenic bacteria in biological fluids].
Use of IHAT with the addition of antiserum to immunoglobulins G, M, A increased the frequency of detection of antibodies to enteropathogenic bacteria in various biological fluids (blood, saliva, human milk, feces). At the same time the antiglobulin test permitted determination of the immunological class of these antibodies. In the substrates where the antibodies were subjected to an intensive action of proteolytic enzymes there was seen a decrease of the efficacy of the modified IHAT, but by results it exceeded the common IHAT. Antiglobulin test with the addition of antisera to immunoglobulins G, M and A could be recommended for the immunological study of the blood sera and of the external secretions for the purpose of the most reliable detection and determination of the immunological class of antibodies to enteropathogenic bacteria.
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial / Q000032:analysis*; D004405:Dysentery, Bacillary / Q000276:immunology; D004756:Enterobacteriaceae Infections / Q000276:immunology*; D005243:Feces / Q000276:immunology; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007410:Intestinal Diseases / Q000276:immunology*; D008895:Milk, Human / Q000276:immunology; D012463:Saliva / Q000276:immunology; D012764:Shigella sonnei
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G
Primenenie antiglobulinovoĭ proby s tsel'iu vyiavleniia v biologicheskikh zhidkostiakh antitel k énteropathogennym bakteriiam