The use of immunoprecipitation to study the synthesis and cleavage processing of viral proteins.
Several factors have been considered in developing an immunoprecipitation procedure which minimizes the background of non-immunospecifically precipitated protein. The procedure evolved utilizes the technique of direct immunoprecipitation (specific antibody plus sufficient unlabeled antigen to form a precipitable complex). High speed centrifugation of the cell extract immediately prior to immunoprecipitation and the use of test tubes coated with silicone greatly reduced background precipitation.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000032:analysis; D002457:Cell Extracts / Q000276:immunology; D011232:Chemical Precipitation; D007106:Immune Sera / Q000032:analysis; D007158:Immunologic Techniques / Q000379:methods*; D051379:Mice; D011925:Rauscher Virus / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D000956:Antigens, Viral; D002457:Cell Extracts; D007106:Immune Sera; D014764:Viral Proteins