Preparation of vascular endothelium for scanning electron microscopy: a comparison of the effects of perfusion and immersion fixation.
A comparison of currently used methods of tissue fixation for scanning electron microscopic study of vascular endothelium revealed that in situ fixation by intravascular perfusion is superior to immersion for the preservation of endothelial surface morphology.
D000818:Animals; D001158:Arteries / Q000648:ultrastructure*; D003584:Cytological Techniques; D004727:Endothelium / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D013552:Swine