Immune response to an allogeneic progressively growing solid tumor.
A C57BL/6 (B6) methylcholanthrene-induced solid tumor grows progressively in BALB/c mice. The BALB/c mice fail to develop in their spleens cytotoxic lymphocytes directed against the histocompatibility (H-) antigens of the tumor. A B6 skin graft is rejected normally by mice with the progressively growing B6 tumor and skin graft rejection has no influence on the growth of the tumor. Tissue culture-carried tumor cells work satisfactorily as target cells in a 51Cr release assay with effector lymphocytes immunized against B6 H-antigens, but the tumor cells will not work as an immunogen for H-antigens for an in vitro immunization. "Fresh" tumor cells removed from their in vivo environment and separated on a discontinuous bovine serum albumin gradient also work as target cells. The conclusion is drawn that the host cytotoxic lymphocyte response against the tumor, as defined by a short-term 51Cr release assay, suffers from both sensitization and effector stage defects that probably do not involve serum factors or suppressor cells.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D006084:Graft Rejection; D006649:Histocompatibility Antigens; D007114:Immunization; D007942:Leukemia, Experimental / Q000209:etiology / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012513:Sarcoma, Experimental / Q000209:etiology / Q000276:immunology*; D016038:Skin Transplantation; D013154:Spleen / Q000276:immunology; D014184:Transplantation, Homologous
D006649:Histocompatibility Antigens