Proteins of yeast ribosomal subunits: number and general properties.
1. Saccharomyces cerevisiae at the early stationary phase of growth accumulate 80 S ribosomes, easily dissociating into subunits, which retain full activity in phenylalanine polymerization in vitro. A simplified and efficient technique for large-scale preparation of yeast ribosomal subunits is proposed. 2. Presence of 34 proteins in 40 S subunit and 42 proteins in 60 S subunit was demonstrated by two-dimensional acrylamide-gel electrophoresis. Both ribosomal subunits contain acidic proteins: three in 60 S and six or seven in 40 S subunit. It seems that two of them correspond to prokaryotic proteins, L7 and L12. The total number of yeast ribosomal proteins is similar to those obtained for other Eukaryota.
D002501:Centrifugation, Zonal; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation; D005656:Fungal Proteins / Q000302:isolation & purification*; D008722:Methods; D012343:RNA, Transfer / Q000032:analysis; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D005656:Fungal Proteins; D012269:Ribosomal Proteins; D012343:RNA, Transfer