Effect of phospholipids on the activity of DNA polymerase I from E. coli.
Sphingomyelin, phosphatidylethanolamine and lecithin at concentrations which destabilize the DNA helix enhance the DNA polymerase activity, but sphingosine and phosphatidylserine have only a moderate effect. MgCl2 was shown to modify the action of sphingomyelin on the DNA polymerase activity. The role of phospholipids in the DNA replicating process was analyzed.
D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology*; D013109:Sphingomyelins / Q000494:pharmacology
D010714:Phosphatidylethanolamines; D010743:Phospholipids; D013109:Sphingomyelins; D004254:DNA Nucleotidyltransferases; D008274:Magnesium