Lysogenization by bacteriophage lambda IV inhibition of phage DNA synthesis by the products of genes cII and cIII.
In direct measurements of phage lambda DNA synthesis, we have detected an inhibition caused by the cII and cIII gene products. This inhibition was more clearly observed when P amber phages were grown in a permissive host, presumably because of the limitation in DNA synthesis due to uncomplete suppression. The inhibition takes place in cells infected at high multiplicity, but not in cells infected at low multiplicity. To explain these findings, we propose a model in which the bacterial population is heterogeneous with respect to its ability to support phage DNA synthesis. An initial limitation caused by host factors would be amplified by the action of the cII and cIII products, at high multiplicity only, and the resulting inhibition would be essential in the "choice" towards lysogeny.
D002874:Chromosome Mapping; D003090:Coliphages / Q000378:metabolism*; D004261:DNA Replication; D004926:Escherichia coli / Q000378:metabolism*; D005796:Genes; D007700:Kinetics; D008242:Lysogeny; D014779:Virus Replication