Rapid diagnosis of mumps virus infections by immunofluorescence methods.
Mumps and its complications, particularly meningoencephalitis, is an important disease problem, and more rapid diagnostic methods are desirable. A study was made of immunofluorescence methods for the early detection of mumps virus isolated in cell cultures, or adsorbed directly from clinical specimens onto guinea pig erythrocytes. A specific diagnosis could be made in hours to 2 or 3 days utilizing immunofluorescence methods, in contrast to about 6 days by standard methods. Details of the direct immunofluorescence methods are presented, to encourage wider application in clinical virology laboratories.
D000818:Animals; D002460:Cell Line; D002555:Cerebrospinal Fluid / Q000382:microbiology; D003937:Diagnosis, Differential; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs / Q000276:immunology; D006801:Humans; D009107:Mumps / Q000175:diagnosis*; D009109:Mumps virus / Q000302:isolation & purification; D010614:Pharynx / Q000382:microbiology; D014556:Urine / Q000382:microbiology; D014776:Virus Cultivation