Rapid detection and isolation of mycoplasmas from cell cultures.
An improved medium for the isolation of Mycoplasma species from contaminated cell cultures is reported. A modification of the mycoplasma detection method using the uridine/uracil uptake ratio method is described. Results obtained using this method with mycoplasma contaminated cell cultures and with contaminated cell cultures treated with the antibiotic Lincomycin are presented.
D000818:Animals; D001431:Bacteriological Techniques; D002478:Cells, Cultured / Q000378:metabolism / Q000382:microbiology*; D003470:Culture Media; D006736:Horses; D006801:Humans; D008034:Lincomycin / Q000494:pharmacology; D009174:Mycoplasma / Q000187:drug effects / Q000302:isolation & purification*; D014498:Uracil / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D003470:Culture Media; D014498:Uracil; D008034:Lincomycin; D014529:Uridine