title: One-tube oxidation-fermentation methods: limitations posed by atypical fermentative reactions.
abstract: Atypical fermentation in the two-tube method of Hugh and Leifson (1953), whereby acid was produced in the sealed tube but not in the open tube, was determined to be the result of a preferential metabolism of peptone over the reactant carbohydrate. These atypical fermentations are only partially detected by one-tube oxidation-fermentation methods, and as such they constitute a source of limitation to the routine use of these abbreviated techniques for oxidative-fermentation determinations.
mesh_terms: D000332:Aerobiosis; D001431:Bacteriological Techniques / Q000295:instrumentation; D002861:Chromobacterium / Q000145:classification / Q000378:metabolism*; D005285:Fermentation; D008320:Maltose / Q000378:metabolism; D010461:Peptones / Q000378:metabolism; D013213:Starch / Q000378:metabolism
chemical_list: D010461:Peptones; D008320:Maltose; D013213:Starch