Testing of some benzidine analogues for microsomal activation to bacterial mutagens.
Analogues of benzidine were assayed for mutagenic activity towards Salmonella typhimurium TA 1538 in the presence and absence of a liver enzyme preparation. Purified 3,3'-dichlorobenzidine and the technical grade material had some direct mutagenic activity, but this was increased over 50-fold by addition of a liver mixed function oxidase preparation. In the presence of the liver preparation, 3,3'-dichlorobenzidine was approximately 10 times more active than benzidine, while 3,3',5,5'-tetrafluorobenzidine was of approximately equipotency. On the other hand, 3,3',5,5'-tetramethylbenzidine had no mutagenic activity alone or in conjunction with a liver preparation. 3,3'-Dianisidine had slight mutagenic activity in the presence of liver but none in its absence.
D015101:3,3'-Dichlorobenzidine / Q000494:pharmacology; D000818:Animals; D001560:Benzidines / Q000378:metabolism / Q000494:pharmacology*; D003962:Dianisidine / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism*; D009153:Mutagens; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects*
D001560:Benzidines; D009153:Mutagens; D015101:3,3'-Dichlorobenzidine; D003962:Dianisidine