A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes.
Mg2+ was shown to affect the conformation of rRNA over the range of 0.03-1.2M-KCl. The species studies were Escherichia coli S-rRNA and L-rRNA (the RNA moieties of the smaller and larger subribosomal particles respectively) and rabbits S-rRNA and L-rRNA. 2. The addition of Mg2+ to rRNA in reconstitution buffer (0.35M-KCl0.01M-Tris/HCl, pH7.2) at 20 degrees C let to an increase in bihelical secondary structure through the formation of additional (mainly A-U) base-pairs (e.g. an additional approx. 58 A-U base-pairs per molecule of E. coli S-rRNA as judged by u.v. difference spectrophotometry...
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D004926:Escherichia coli / Q000648:ultrastructure; D008274:Magnesium / Q000494:pharmacology*; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D011189:Potassium Chloride / Q000494:pharmacology; D012335:RNA, Ribosomal; D011817:Rabbits; D012156:Reticulocytes / Q000648:ultrastructure; D012270:Ribosomes / Q000187:drug effects*; D013056:Spectrophotometry, Ultraviolet
D012335:RNA, Ribosomal; D000643:Ammonium Chloride; D011189:Potassium Chloride; D008274:Magnesium