Classification of smooth muscle autoantibodies detected by immunofluorescence.
Three hundred and twelve sera containing antibodies to smooth muscle (SMA) wer analysed for the immunofluorescence patterns they produced in various tissues. A classification is described based on the three main appearances in rat kidney. Some sera, mainly of low titre, reacted only with vessel walls (SMA-V), some stained vessels and renal glomeruli (SMA-G) and high titre sera, mainly from patients with chronic active hepatitis stained vessels, glomeruli, and intracellular fibrils in renal tubules (SMA-T). Peripheral staining in hepatocytes or thyroid cells was not a regular feature. 41/43 polyclonal SMA-T and -G sera were absorbed out completely by actin, and this also removed the pericullular staining in liver and thyroid when present. High titre SMA-V antibodies could not be absorbed by actin, and the antigen remains to be identified.
D000818:Animals; D001323:Autoantibodies / Q000032:analysis / Q000145:classification*; D002417:Cattle; D005455:Fluorescent Antibody Technique; D006505:Hepatitis / Q000276:immunology; D006801:Humans; D007668:Kidney / Q000276:immunology; D007678:Kidney Glomerulus / Q000276:immunology; D007684:Kidney Tubules / Q000276:immunology; D008099:Liver / Q000276:immunology; D008107:Liver Diseases / Q000276:immunology; D051379:Mice; D009124:Muscle Proteins / Q000276:immunology; D009130:Muscle, Smooth / Q000276:immunology*; D009132:Muscles / Q000276:immunology; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D013961:Thyroid Gland / Q000276:immunology
D001323:Autoantibodies; D009124:Muscle Proteins