Improved method of purifying some radiolabeled glycopeptide hormones.
We describe a simplified method for purifying radiolabeled glycopeptide hormones (thyrotropin, lutropin, follitropin, and human choriogonadotropin), which results in preparations of over 95% immunoreactivity. This method is based on competitive elution from columns of the plant lectin, concanavalin A, and is applicable to glycopeptide hormones derived from several species.
D006063:Chorionic Gonadotropin / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A; D005640:Follicle Stimulating Hormone / Q000302:isolation & purification; D006020:Glycopeptides / Q000302:isolation & purification*; D006728:Hormones / Q000302:isolation & purification*; D007553:Isotope Labeling / Q000379:methods; D007986:Luteinizing Hormone / Q000302:isolation & purification; D013972:Thyrotropin / Q000302:isolation & purification
D006063:Chorionic Gonadotropin; D006020:Glycopeptides; D006728:Hormones; D003208:Concanavalin A; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin