The use of agarose gels for the assay of the deoxyribonuclease activity associated with the soluble penton of adenoviruses.
Evidence is presented that the excess pool of penton complexes purified from adenovirus type 1-infected HEp-2 cells also carry an endonucleolytic activity. The effect was measured with both E. coli and adenovirus type 1 double-stranded DNA substrates. The use of agarose gels for assaying the endonuclease activity allowed the approximative calculation of the number of penton molecules which might produce one DNA double-strand break under the experimental conditions selected.
D000260:Adenoviruses, Human / Q000201:enzymology* / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D006868:Hydrolysis; D012995:Solubility; D014764:Viral Proteins / Q000378:metabolism*
D004269:DNA, Bacterial; D004279:DNA, Viral; D014764:Viral Proteins; D003851:Deoxyribonucleases; D004720:Endonucleases