[New methods of determination of the functional peculiarities of autoantibodies in various types of autoimmune reactions].
Anderson's method (with our modification of Jerne's method) was applied in experiments on 574 albino female mice; a study was made of the affinity of autoantibodies in three groups of animals: intact, gamma (Co60) irradiated in a dose of 600 r, and with stimulation of auto-immune reaction by injection of a complete adjuvant (0.05 ml subcutaneously) or injection of living E. coli cells (subcutaneously 300 million) or hemostimulation (subcutaneously 0.1--0.3 or 0.5 ml of homologous blood). The maximal autoantibody indices were obtained in the irradiated mice, the least -- in the intact animals. Apart from the plaque-formation inhibition phenomenon, there were observed two more phenomena; these were an immune erythrocyte adherence to the immunocompetent cells and a marked increase in the formation of plaques of autoimmune hemolysis in the preparation with addition of diluted (1:10) lysate of erythrocytes obtained from mice with the administration of an adjuvant or homologous blood.
D000818:Animals; D001323:Autoantibodies / Q000032:analysis; D004927:Escherichia coli Infections / Q000276:immunology; D005260:Female; D005620:Freund's Adjuvant; D006462:Hemolytic Plaque Technique; D007104:Immune Adherence Reaction; D051379:Mice; D011833:Radiation Injuries, Experimental / Q000276:immunology*
D001323:Autoantibodies; D005620:Freund's Adjuvant
O novykh metodakh vyiavleniia funktional'nykh osobennosteĭ autoantitel pri razlichnykh vidakh autoimmunnykh reaktsiĭ