Reprecipitation phenomena arising during the preparation of demineralised sections. III Scanning electron microscopic examinations of secondary calcium phosphate deposits.
Sections of teeth partly demineralized in 10% formic acid were examined by X-ray diffraction, microradiography and scanning electron microscopy. In the undemineralized circumpulpal dentin, the tubules were empty, lying in a matrix containing hydroxyapatite. In the "plume" areas of remineralisation, the tubules were filled with mineral deposits. X-ray diffraction revealed the presence of brushite and monetite in these areas. In the outer layers of dentin the tubules were empty, lying in a matrix containing some residual hydroxyapatite. These findings confirmed that the remineralisation process occurred within the dentinal tubules.
D002130:Calcium Phosphates; D003648:Decalcification Technique; D003804:Dentin / Q000648:ultrastructure*; D006801:Humans; D066298:In Vitro Techniques; D008852:Microradiography; D008855:Microscopy, Electron, Scanning; D014070:Tooth / Q000000981:diagnostic imaging; D014074:Tooth Calcification; D014961:X-Ray Diffraction
D002130:Calcium Phosphates