Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro.
E. coli dnaZ gene product is required for conversion of phiX174, fd, and ST-1 single-stranded phage DNAs to duplex DNAs in vitro. This protein has been purified about 5000-fold. It functions in the elongation of RNA- or DNA-primed single-stranded DNA that is catalyzed by DNA polymerase III(DNA nucleotidyltransferase; deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase; EC 2.7.7.7) in conjunctions with two other E. coli protein preparations referred to as DNA elongation factors I and III. It also functions in similar reactions catalyzed by DNA polymerase II in combination with E. coli DNA binding protein and DNA elongation factors I and III.
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002474:Cell-Free System; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005796:Genes
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004254:DNA Nucleotidyltransferases