Preparation of rabies fluorescein isothiocyanate-labeled immune globulin from mouse hyperimmune ascitic fluids.
Immunization conditions for the production of mouse immune ascitic fluids to be used for the preparation of rabies fluorescent antibody (FA) conjugate are presented. The use of optimal concentrations of ammonium sulfate for precipitation of gamma globulin resulted in a fraction consisting of 75% gamma globulin and 25% alpha-beta globulins with no detectable albumin. Dialysis labeling of the globulin fration with fluorescein isothiocyanate produced a specific rabies FA conjugate with negligible nonspecific background staining. This procedure represents a simple means of producing rabies FA conjugate.
D000818:Animals; D000914:Antibodies, Viral; D001202:Ascitic Fluid / Q000276:immunology*; D001921:Brain / Q000276:immunology; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D007120:Immunochemistry; D007136:Immunoglobulins; D051379:Mice / Q000276:immunology; D011818:Rabies / Q000175:diagnosis; D011820:Rabies virus / Q000276:immunology*
D000914:Antibodies, Viral; D005452:Fluoresceins; D007136:Immunoglobulins