title: Kinetics of the early insulin response of the perfused rat pancreas to various metabolites and tolbutamide.
abstract: The collection every 15 seconds of the portal effluent of the isolated and perfused rat pancreas permitted the following observations: while the insulin response to tolbutamide (250 or 125 mug/ml) occurred immediately, that to glucose (15 mM), or to 1-leucine (15 mM) was delayed by 60-90 seconds. The response to d-glyceraldehyde occurred slightly but significantly more quickly than that of glucose. The response to alpha-keto-isocaproate was an intense and immediate one. Isovalerate elicited no insulin discharge. These results suggest that d-glucose and l-leucine have to be metabolized to stimulate insulin secretion and that alpha-keto-isocaproate seems to be a direct and specific signal.
mesh_terms: D000818:Animals; D002208:Caproates / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D005985:Glyceraldehyde / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007930:Leucine / Q000494:pharmacology*; D008297:Male; D051381:Rats; D013268:Stimulation, Chemical; D013997:Time Factors; D014044:Tolbutamide / Q000494:pharmacology*; D014631:Valerates / Q000494:pharmacology*
chemical_list: D002208:Caproates; D007328:Insulin; D014631:Valerates; D005985:Glyceraldehyde; D014044:Tolbutamide; D007930:Leucine; D005947:Glucose