title: Indirect selection for auxotrophic mutants of Saccharomyces cerevisiae using the antibiotic netropsin.
abstract: The small basic oligopeptide antibiotic, netropsin, can be successfully employed as an effective counterselecting agent in Saccharomyces cerevisiae. The use of the drug results in approximately a 35-fold enrichment of auxotrophic mutants in a mutagenized culture of yeast. The experimental procedure is quite simple and less time consuming than other presently used methods for indirect mutant selection in yeast.
mesh_terms: D000906:Antibodies / Q000494:pharmacology*; D005821:Genetic Techniques; D006146:Guanidines / Q000494:pharmacology*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development; D012641:Selection, Genetic; D013997:Time Factors
chemical_list: D000906:Antibodies; D006146:Guanidines