In vitro system for induction of delayed early RNA of bacteriophage T4.
Concentrated lysates of Escherichia coli that had been infected with bacteriophage T4 in the presence of chloramphenicol show the same restriction of transcription in vitro as is found in vivo. Restricted lysates can be complemented with lysates from infected cells to induce production of delayed early RNA. Complementation takes place between the RNA polymerase of the restricted lysate and the DNA of the unrestricted lysate. We present evidence that delayed early RNA in these lysates is initiated at quasi-late (middle) promoters, and that such recognition is related to changes in the state of the template DNA.
D002474:Cell-Free System; D002701:Chloramphenicol / Q000494:pharmacology; D003090:Coliphages / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000648:ultrastructure; D005796:Genes; D005809:Genes, Regulator; D008566:Membranes / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis*; D012293:Rifampin / Q000494:pharmacology; D013997:Time Factors
D004279:DNA, Viral; D012367:RNA, Viral; D002701:Chloramphenicol; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin