title: Liquid holding recovery in stationary and log phase cultures of diploid yeast exposed to gamma and alpha radiations.
abstract: The kinetics of liquid-holding recovery (LHR) in diploid yeast after gamma and alpha irradiation is studied. In case of stationary phase culture the rate and extent of LHR is found to be greater for gamma-ray-induced damage than for alpha-ray-induced damage. At 10% survival level, the half-time for recovery is 5.2 h for gamma-ray damage and 12 h for alpha-ray damage. Further, while the recovery factor for alpha damage reaches saturation at 5% survival level, that for gamma damage continues to increase as survival level decreases. Oxygen is required for the recovery process during LH after gamma irradiation. The cells can recover to the same extent from both oxygen-dependent and oxygen-independent components of damage. Log phase cells containing a high per cent of budding cells, however, exhibit negative liquid holding effect after gamma irradiation.
mesh_terms: D000512:Alpha Particles; D003037:Cobalt Radioisotopes; D003476:Curium; D004260:DNA Repair; D004171:Diploidy; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D010100:Oxygen; D012441:Saccharomyces cerevisiae / Q000528:radiation effects*
chemical_list: D003037:Cobalt Radioisotopes; D003476:Curium; D010100:Oxygen