Enrichment in spontaneous and complement dependent rosette forming cell populations using Ficoll-Hypaque gradient centrifugation.
Peripheral blood human T (TL) and B (BL) lymphocytes were separated by continuous Ficoll-Hypaque (FH) gradients. BL were found at the 1050 density interfase (70.5% EAC rosettes) with no TL (0% EAC rosettes); while at the 1068 density interfase there was an enrichment of TL (68% E rosettes) with very few BL (8.5% EAC rosettes).
D001402:B-Lymphocytes; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D005362:Ficoll; D006801:Humans; D007158:Immunologic Techniques; D013601:T-Lymphocytes
D005362:Ficoll