Synergistic effect of alloantibodies or F(ab')2 and prednisone on murine split heart allograft survival.
Heterotopic grafting of split allogeneic mouse hearts across a strong H-2 barrier (A/Sn leads to A.CA) was used in a study of alloantibody or F (ab')2 induced enhancement. Strong specific binding of alloantibodies occurred in the graft. A marked synergistic effect on graft survival was observed after combined treatment of recipients with enhancing serum and prednisone. The highly dose-dependent enhancing effect of F (ab')2 was also strongly potentiated by supporting prednisone treatment. In vitro pre-incubation of the heart tissue with alloantiserum or F (ab')2 did not prolong graft survival; in contrast, C-mediated damage of the graft was suggested when serum was used.
D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D003601:Cytotoxicity Tests, Immunologic; D006084:Graft Rejection / Q000517:prevention & control*; D016027:Heart Transplantation; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007518:Isoantibodies; D051379:Mice; D011241:Prednisone / Q000627:therapeutic use*; D013997:Time Factors; D014022:Tissue Survival; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007518:Isoantibodies; D011241:Prednisone