Enzymic analysis of bile acids.
1. The use of enzymes and thin-layer chromatography (TLC) in quantifying individual bile acids from bile or serum is reviewed. 2. Both 3alpha-hydroxysteroid dehydrogenase (commercially available P. testosteroni enzyme) and 7alpha-hydroxysteroid dehydrogenase (E. coli) can be used for spectrophotometric or fluorometric measurement of individual bile acids in TLC eluates and to estimate pool size and half-life of serially labelled samples.
D001647:Bile Acids and Salts / Q000097:blood*; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases; D008722:Methods; D011549:Pseudomonas / Q000201:enzymology; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D001647:Bile Acids and Salts; D006913:Hydroxysteroid Dehydrogenases