Antibodies of surface antigens of lymphocytes and lymphoblastoid cells in cold-agglutinin-positive sera from patients with Mycoplasma pneumoniae infection.
IgM antibodies reacting in the cold with surface antigens of normal human blood lymphocytes were demonstrated by indirect immunofluorescence (IFL) in cold-agglutinin-positive sera from 21 patients with Mycoplasma pneumoniae (MP) infection. Twenty to fifty per cent of the lymphocytes were stained. MP sera reacted also with 75%-100% of cells from two lymphoblastoid cell lines and one Burkitt lymphoma line and with about 80% of peripheral blood lymphocytes from one patient with chronic lymphatic leukaemia. The IFL reaction was negative with cells from leukaemic lymphoid T-cell line (Molt-4). Lymphocyte fractionation experiments gave no evidence of a selective reactivity of MP sera and B or T lymphocytes of peripheral blood. Removal of cold agglutinins from the MP sera by absorption with human O erythrocytes significantly reduced the IFL reactivity with lymphocytes and lymphoblastoid cells, indicating the presence of I antigen on these cells. Furthermore, lymphoblastoid cells but not Molt lymphoid cells absorbed out most of the cold erythroagglutinins.
D000373:Agglutinins / Q000276:immunology; D000906:Antibodies / Q000032:analysis; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis*; D002051:Burkitt Lymphoma / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D003080:Cold Temperature; D005455:Fluorescent Antibody Technique; D006801:Humans; D007048:I Blood-Group System; D007075:Immunoglobulin M / Q000032:analysis*; D037102:Lectins / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013997:Time Factors; D014373:Tuberculin / Q000494:pharmacology
D000373:Agglutinins; D000906:Antibodies; D000941:Antigens; D007048:I Blood-Group System; D007075:Immunoglobulin M; D037102:Lectins; D011947:Receptors, Antigen, B-Cell; D014373:Tuberculin