[Bactericidal power of polymorphonuclear neutrophils. Trials for standardization of a micromethod using autoradiography (author's transl)].
A 50 mul sample of blood is placed successively in the presence of staphylococci, then of tritiated thymidine. Stripping film autoradiography permits identification of living intracellular bacteria which metabolize the marked precursor. In our experimental conditions, the percentage of marked intracellular bacteria is: -- 15,4 +/- 3,7% (x +/- 1s) among adults -- 14,6 +/- 3,2% among children.
D000328:Adult; D000900:Anti-Bacterial Agents; D001345:Autoradiography / Q000379:methods* / Q000592:standards; D001769:Blood / Q000382:microbiology; D001770:Blood Bactericidal Activity; D002648:Child; D004352:Drug Resistance, Microbial; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000502:physiology*; D013211:Staphylococcus aureus; D013223:Statistics as Topic
D000900:Anti-Bacterial Agents
Pouvoir bactéricide des polynucléaires neutrophiles. Essai de standardisation d'une microméthode utilisant l'autoradiographie