Use of a sequence-specific DNA-binding ligand to probe the environments of EcoRI restriction endonuclease cleavage sites.
The DNAs of bacteriophage lambda and adenovirus were incubated with the sequence-specific DNA-binding ligand 6,4'-diamidino-2-phenylindole. Digestion of the ligand-DNA complexes with EcoRI nuclease and subsequent agarose gel electrophoresis demonstrated that the ligand inhibited nuclease activity at some sites, but not at others. The results suggest that diamidino-2-phynylindole can be used to probe the immediate environments of the EcoRI cleavage sites.
D000256:Adenoviridae; D001665:Binding Sites; D002875:Chromosomes; D003090:Coliphages; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D007211:Indoles; D007700:Kinetics; D008024:Ligands
D004279:DNA, Viral; D007211:Indoles; D008024:Ligands; D004720:Endonucleases; D004262:DNA Restriction Enzymes