In vitro C-20, 22 desmolase regulation of pregnenolone supply in first trimester human placenta.
Homogenates of first trimester human placentae were incubated with [26-14C]chloresterol. Using reverse-isotope dilution analysis [14C]isocaproic acid was isolated and characterized. A progressive increase in [14C]isocaproic acid formation with increasing gestation suggestive of a regulatory mechanism at the C-20, 22 desmolase site in the supply of placental pregnenolone was noted.
D002208:Caproates / Q000096:biosynthesis / Q000302:isolation & purification; D002784:Cholesterol / Q000378:metabolism; D003460:Crystallization; D005260:Female; D006801:Humans; D008190:Lyases / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011284:Pregnenolone / Q000378:metabolism*
D002208:Caproates; D011284:Pregnenolone; D002784:Cholesterol; D008190:Lyases