Evidence against autorepression of the betabeta' operon in Escherichia coli.
By using rifampicin to increase the rate of beta and beta' synthesis in a heterodiploid strain of E. coli carrying the mutation rifpr (Km7), which codes for a rifampicin sensitive RNA polymerase to which the drug binds weakly, and the dominant mutation rifRD, which codes for a rifampicin resistant RNA polymerase, the concentration of these subunits in the cell was increased 1.6 fold. Measurements made after removal of rifampicin from the cells showed that the excess beta and beta' subunits did not reduce the rate of their own synthesis below normal.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects*; D005799:Genes, Dominant; D005809:Genes, Regulator; D006706:Homeostasis; D009154:Mutation; D009876:Operon / Q000187:drug effects*; D012293:Rifampin / Q000494:pharmacology*
D012321:DNA-Directed RNA Polymerases; D012293:Rifampin