Incomplete viral synthesis in Friend leukemia virus-induced reticulum cell sarcomas.
Tissue-culture-passaged, Friend leukemia virus (FV)-induced reticulum cell sarcomas from BALB/c mice (FVTCT-BALB) did not produce infectious FV, although retrieval of infectious FV occurred when these cells were co-cultivated with cell lines replicating non-defective murine leukemia viruses (MLVs). The level of FV expression in the FVTCT-BALB cell line was studied to understand better the process of FV retrieval. 3H-uridine labelling techniques and reverse transcriptase assays showed that FVTCT-BALB cells did not release C-type virus particles. Nucleic acid hybridization techniques demonstrated that the level of viral RNA synthesis in the FVTCT-BALB non-producer cell line was indistinguishable from that in cell lines productively infected with MLVs. These data suggest that in the FVTCT-BALB cell line the synthesis of FV is blocked in some late stage of virus assembly.
D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005622:Friend murine leukemia virus / Q000254:growth & development*; D049671:History, 18th Century; D016403:Lymphoma, Large B-Cell, Diffuse / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis*; D012513:Sarcoma, Experimental / Q000382:microbiology; D014779:Virus Replication
D004279:DNA, Viral; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases