Dynamics of thymidine incorporation by spleen cells from rats infected with Plasmodium berghei.
The in vitro incorporation of thymidine by spleen cells of rat infected with Plasmodium berghei was higher than that of normal rat spleen cells. The incorporation was proportional to the number of cells in the system and was related to the day of infection, e.g. spleen cells from malarious rats incorporated up to forty times as much thymidine as did normal spleen cells 21 days after inoculation. At the same time, the response of the malarious spleen cells to phytohaemagglutinin (PHA) was only one-twentieth of that observed for normal spleen cells. The thymidine was incorporated by non-adherent spleen cells in a single pulse during the first 8 hr in culture.
D000818:Animals; D002448:Cell Adhesion; D066298:In Vitro Techniques; D037102:Lectins; D008213:Lymphocyte Activation; D008288:Malaria / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D010962:Plasmodium berghei; D051381:Rats; D013154:Spleen / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D013936:Thymidine / Q000378:metabolism*
D037102:Lectins; D013936:Thymidine