Demonstration of adenovirus associated endonuclease.
In HEp-2 and amnion cell cultures infected with type 1 adenovirus the DNase activity of cell extracts was measured on 32P-labelled Escherichia coli DNA substrate. Enzyme activity was demonstrated by the acid soluble nucleotides released from the 32P-DNA and by the decreased sedimentation rate of labelled DNA. High DNase activity was measured in both adenovirus infected and in untreated HEp-2 cell extracts. Nuclease activity of the amnion host cells being much lower than that of HEp-2 cells, virus-associated endonuclease activity was successfully demonstrated in them. Purified type 1 adenovirus decreased the sedimentation rate of 32P-labelled E. coli DNA. The phenomenon is explained by the virion-associated endonuclease activity. Trypsin inactivated and anti HEp-2 IgG failed to inhibit the virion nuclease. An association between endonuclease and trypsin sensitive penton is assumed.
D000256:Adenoviridae / Q000201:enzymology* / Q000254:growth & development; D002460:Cell Line; D002474:Cell-Free System; D046508:Culture Techniques; D004269:DNA, Bacterial / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D004926:Escherichia coli; D014357:Trypsin / Q000494:pharmacology
D004269:DNA, Bacterial; D003851:Deoxyribonucleases; D004720:Endonucleases; D014357:Trypsin