Phenylalanyl-tRNA synthetase from baker's yeast: specificity and quantitation of affinity elution with tRNA.
TRNAPhe is able to elute phenylalanyl-tRNA synthetase from cation exchangers in a 1:1 ratio. Elution of phenylalanyl-tRNA synthetase in a 1:1 ratio is also observed for four noncognate tRNAs investigated, specific for valine, serine, isoleucine and tyrosine. If protein mixtures are subjected to affinity elution the cognate pair [tRNAPhe-phenylalanyl-tRNA synthetase] is eluted first, followed by noncognate pairs. The unspecific elution is not influenced by complexation of phenylalanyl-tRNA synthetase with an analog of phenylalanyl-adenylate.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004586:Electrophoresis; D005656:Fungal Proteins / Q000302:isolation & purification; D010652:Phenylalanine-tRNA Ligase / Q000302:isolation & purification*; D012343:RNA, Transfer / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012967:Sodium Dodecyl Sulfate
D005656:Fungal Proteins; D012967:Sodium Dodecyl Sulfate; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase