[Method for the intavital of the isolated pancreatic islands of Langerhans in rats].
The authors describe a method of vital detection and isolation of individual islands of Langerhans in rats. This method is based on the capacity of the beta-cells of the islands to accumulate the stain during life. Since the stain (0.05% neutral red solution) is harmless and it is rapidly eliminated by the cells this method permits vital marking on the surface of the pancreas of individual islands and their distinguishment under steroscopic microscope; these islands can be isolated from the surrounding parenchyma of the pancreas with the aid of a microknife. Thus obtained islands of the pancreas could be used as material for experiments with implantation and for other experimental studies.
D000818:Animals; D003584:Cytological Techniques; D007515:Islets of Langerhans / Q000166:cytology*; D008853:Microscopy; D009499:Neutral Red; D051381:Rats; D012394:Rosaniline Dyes; D013997:Time Factors
D012394:Rosaniline Dyes; D009499:Neutral Red
Metod prizhiznennogo vyiavleniia izolirovannykh ostrovkov Langergansa v podzheludochnoĭ zheleze u krys