title: RNA and protein synthesis in human peripheral blood polymorphonuclear leukocytes.
abstract: Polymorphonuclear leukocytes purified from human peripheral blood synthesized RNA and proteins when placed in cell culture. Autoradiography of the cultured cells revealed that a majority of mature PMNs were engaged in macromolecule synthesis, and an analysis of newly synthesized proteins by SDS-polyacrylamide gel electrophoresis showed that many different polypeptide chains were synthesized by these cells. The rate of [3H]uridine incorporation and the pattern of newly synthesized proteins were modulated by Con A and glucocorticoids. These results suggest that in spite of their short lifetime and a large performed enzymatic apparatus, mature PMNs retain a substantial capacity for RNA and protein synthesis; and, further, that modulation of macromolecule synthesis forms part of the mechanism by which PMNs respond to inflammatory and anti-flammatory stimuli.
mesh_terms: D001798:Blood Proteins / Q000096:biosynthesis*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*
chemical_list: D001798:Blood Proteins; D003208:Concanavalin A; D012313:RNA; D003907:Dexamethasone