Comparison of chemical and immunological characteristics of some commercially obtainable conjugates.
A method is suggested for the chemical and immunological characterization of commercially obtainable conjugates. 19 antihuman IgG; IgA; IgM and beta1A-beta1C conjugates of various origine were compared by means of the method. The most surprising characteristics of the samples were the great differences in that quality. Experiments carried out with conjugates of poor quality will produce doubtful or contradictory results. Reliable characterization of conjugates is an essential and important part of immunofluorescent examination.
D000906:Antibodies / Q000032:analysis; D004396:Coloring Agents / Q000032:analysis; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D011506:Proteins / Q000032:analysis; D012997:Solvents
D000906:Antibodies; D004396:Coloring Agents; D005452:Fluoresceins; D011506:Proteins; D012997:Solvents