Application of the enzymic electric cell method to the activity assay of NAD-linked dehydrogenases.
An enzymic electric cell was constructed with a saturated calomel electrode (cathode) and an enzymic electrode (anode) which consisted of a glassy carbon electrode and a mixture containing an NAD-linked dehydrogenase, NAD+, Nomethylphenazonium methosulfate and a substrate, and the short-circuit current of the cell was measured...
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D004560:Electricity / Q000295:instrumentation; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008722:Methods; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013552:Swine
D000429:Alcohol Oxidoreductases; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases