Genetic characterization of a phi80 transducing bacteriophage carrying the histidine operon of Salmonella typhimurium.
A phi 80 transducing phage, phi 80imm lambdadhis, carrying the Salmonella his-gnd region, was characterized by immunity studies, tonB deletion analysis, and marker rescue analysis. Phi 80imm lambdadhis retains the phage immunity region of the phi 80-lambda hybrid phage from which it was derived. Bacterial genes replace most late phage genes. Deletion analysis shows the prophage gene order to be immlambda-his-gnd and indicates the orientation of the his operon to be hisOGDCBHAFIE-gnd. The structure of phi 80imm lambdadhis is remarkably similar to two independently isolated phi 80 phages that carry the his-gnd region of Escherichia coli and that, like phi80imm lambdahis, were derived by directed gene transposition to the tonB locus. A derivative of phi 80imm lambdadhis that is phi 80 immune is also reported.
D002874:Chromosome Mapping; D003090:Coliphages / Q000378:metabolism; D005796:Genes; D006639:Histidine / Q000096:biosynthesis*; D008242:Lysogeny; D009876:Operon; D012486:Salmonella typhimurium / Q000378:metabolism; D014161:Transduction, Genetic
D006639:Histidine