Genetic and biochemical studies of phosphomannose isomerase deficient mutants of Saccharomyces cerevisiae.
Three mannose-negative mutants of Saccharomyces cerevisiae have been isolated. These mutants showed growth inhibition when mannose was added to a growth medium containing glycerol or fructose. Crosses between wild type mutants showed segregation of 2+/2-. Crosses between the mutants themselves showed that they were closely linked. Two mutants (XM3 and D2) showed characteristics of allelic structural alteration of phosphomannoseisomerase. Mutant D4 had a deficiency of phosphomannoseisomerase activity, but with a normal thermostability. Revertants from D4 had a normal thermostability.
D000225:Adenine / Q000378:metabolism; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D004171:Diploidy; D005947:Glucose / Q000378:metabolism; D008358:Mannose / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification
D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D002238:Carbohydrate Epimerases; D005947:Glucose; D000225:Adenine; D008358:Mannose