Temperature-sensitive mutants of the slime mould Physarum polycephalum. II. Mutants of the plasmodial phase.
Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised amoebae and the properties of these are described. A scheme is outlined for the detection of specific mitotic cycle lesions amongst temperature-sensitive strains, and the properties of a presumptive mitotic cycle mutant are described.
D002455:Cell Division; D005821:Genetic Techniques; D005838:Genotype; D006358:Hot Temperature; D009154:Mutation; D009235:Myxomycetes; D010804:Physarum