Isolation and analysis of the lipid A backbone. Lipid A structure of lipopolysaccharides from  various bacterial groups.
A degradation procedure of lipopolysaccharides was worked out which allows the isolation of the reduced backbone of lipid A in a total yield of between 20 and 30%. This procedure was applied to lipopolysaccharides of S forms (Salmonella minnesota, Shigella flexneri 5b, Escherichia coli 086, E. coli 0111, Xanthomonas sinensis, Rhodopseudomonas gelatinosa) and R mutants (Salmonella minnesota, Shigella flexneri, 5b, E. coli BB9 and E. coli EH 100). Chemical analysis, reaction with beta-N-acetyl-glucosaminidase and application of methylation analysis revealed that the lipid A backbone of all strains contains beta 1', 6-linked glucosamine disaccharides carrying two phosphate groups, one in glycosidic and one in ester linkage, a structure, identified previously in the Salmonella minnesota Re mutant.
D000117:Acetylglucosamine / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D012241:Rhodopseudomonas / Q000032:analysis; D012475:Salmonella / Q000032:analysis; D012763:Shigella flexneri / Q000032:analysis; D013045:Species Specificity; D013403:Sugar Phosphates / Q000032:analysis; D014974:Xanthomonas / Q000032:analysis
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D013403:Sugar Phosphates; D005944:Glucosamine; D000117:Acetylglucosamine