Effects of dicyclohexylcarbodi-imide on proton translocation coupled to fumarate reduction in anaerobically grown cells of Escherichia coli K-12.
The addition of dicyclohexylcarbodi-imide to anaerobic cells of Escherichia coli K12 decreases both the observed extent of proton translocation coupled to fumarate reduction by endogenous substrates and the t 1/2 of proton re-entry after such translocation, but does not affect fumarate uptake. Dicyclohexylcarbodi-imide also inhibits fumarate reductase activity in cell extracts.
D000693:Anaerobiosis; D002234:Carbodiimides / Q000494:pharmacology*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects; D004926:Escherichia coli / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism*; D011522:Protons
D002234:Carbodiimides; D005650:Fumarates; D011522:Protons; D004024:Dicyclohexylcarbodiimide