Interaction of platelets with purified macromolecules of the arterial wall.
A method is described for the study of the interaction between human blood platelets and intercellular matrix macromolecules of connective tissues such as those found in the sub-endothelium. The method is based on the qualitative and quantitative evaluation of the elution pattern of a platelet suspension from a mixed Sepharose 2B-fibrous protein column containing standardized amounts of fibrous proteins such as elastin or structural glycoprotein-microfibrils (SGP-MF). No interaction could be demonstrated with this method between platelets and SGP-MF but there was a very distinct interaction between platelets and elastin fibers. This could be confirmed by direct microscopical demonstration of platelet clusters around elastin fibers in cryostat sections of the affinity column.
D001158:Arteries / Q000502:physiology*; D001792:Blood Platelets / Q000502:physiology*; D002845:Chromatography; D002846:Chromatography, Affinity; D003094:Collagen / Q000502:physiology; D003238:Connective Tissue / Q000502:physiology; D004549:Elastin / Q000502:physiology*; D006023:Glycoproteins / Q000502:physiology*; D006801:Humans; D046911:Macromolecular Substances / Q000502:physiology; D010974:Platelet Aggregation
D006023:Glycoproteins; D046911:Macromolecular Substances; D003094:Collagen; D004549:Elastin