title: Alterations in small-bowel microflora in acute intestinal obstruction.
abstract: Small-bowel content was examined bacteriologically whilst fresh and after storage for 24, 48, and 72 h in a modified Stuart's medium. There was little alteration in the viable count of individual intestinal organisms. For accurate quantitative analysis, 0-3 ml of intestinal content was enough. Specimens of small-bowel content were obtained by needle aspiration, and a qualitative and quantitative study was made of the microflora of patients with acute intestinal obstruction and of a control group of patients. Results in the control group confirmed the findings of the results of intubation studies by other workers, that a quantitative gradient of aerobic and anaerobic organisms exists from jejunum to distal ileum. In acute small-bowel obstruction and acute large-bowel obstruction there was a loss of the normal gradient and an increase in the absolute numbers of organisms present; this was particularly marked for anaerobic organisms in large-bowel obstruction.
mesh_terms: D000208:Acute Disease; D000332:Aerobiosis; D000693:Anaerobiosis; D001419:Bacteria / Q000302:isolation & purification*; D001439:Bacteroides / Q000302:isolation & purification; D002452:Cell Count; D003013:Clostridium / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D005658:Fungi / Q000302:isolation & purification; D006801:Humans; D007082:Ileum / Q000382:microbiology; D007415:Intestinal Obstruction / Q000382:microbiology*; D007420:Intestine, Large; D007421:Intestine, Small / Q000382:microbiology*; D007583:Jejunum / Q000382:microbiology; D007778:Lactobacillus / Q000302:isolation & purification; D013210:Staphylococcus / Q000302:isolation & purification; D013291:Streptococcus / Q000302:isolation & purification