[Technics for isolation of ATP from microorganisms].
A technique for isolation of ATP from microorganisms is suggested. The technique is based on increasing permeability of the cell membrane for substances of low molecular weight during dehydration. Treatment of the dry biomass with boiling water gives a higher yield of ATP from the cells of eukaryote and prokaryote microorganisms as compared with other techniques, and permits to register the amount of APT at the moment of experiment.
D000255:Adenosine Triphosphate / Q000302:isolation & purification*; D001395:Azotobacter / Q000032:analysis; D001419:Bacteria / Q000032:analysis*; D002175:Candida / Q000032:analysis; D002463:Cell Membrane Permeability; D003454:Cryptococcus / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis; D005658:Fungi / Q000032:analysis*; D006358:Hot Temperature; D008722:Methods; D012441:Saccharomyces cerevisiae / Q000032:analysis; D004718:Saccharomycetales / Q000032:analysis
D000255:Adenosine Triphosphate
O metodakh vydeleniia ATF iz mikroorganizmov