Fractionation of mannose-labeled neutral glycopeptides by QAE-Sephadex chromatography.
Mannose-labeled cellular glycopeptides derived from human diploid fibroblasts (KL-2) were separated into two classes by QAE-Sephadex chromatography. High-voltage paper electrophoresis and Sephadex G-50 chromatography were used to characterize the glycopeptides further. At least five distinct neutral fractions were isolated with molecular weights ranging from 1050 to 2000 daltons. A linear gradient of ammonium acetate eluted a heterogeneous population of acidic glycopeptides. The use of QAE-Sephadex enables a single-step fractionation of both neutral and acidic glycopeptides on one column. The technique appears to be sensitive enough to distinguish growth-dependent alterations between growing and non-growing cells.
D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004590:Electrophoresis, Paper; D005347:Fibroblasts / Q000032:analysis; D006020:Glycopeptides / Q000302:isolation & purification*; D006801:Humans; D008358:Mannose / Q000032:analysis; D008722:Methods
D006020:Glycopeptides; D008358:Mannose