Role of modified nucleosides in tRNA: effect of modification of the 2-thiouridine derivative located at the 5'-end of the anticodon of yeast transfer RNA Lys2.
Yeast tRNA Lys2 codes preferentially for AAA and contains a 2-thiouridine derivative (U) at the 5'-position of the anticodon. Removal of the 2-thio group from U by treatment with CNBr did not affect the amino acid accepting activity of the modified tRNA Lys2. CNBr treated tRNA Lys2 was active in protein synthesis but with a much reduced efficiency. Although the modified tRNA Lys2 was recognized by elongation factor (EF) T, the EFT dependent binding to ribosomes to tRNA Lys2 (CNBr) was markedly decreased.
D000926:Anticodon; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D008239:Lysine; D010441:Peptide Chain Elongation, Translational; D010445:Peptide Elongation Factors; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism; D012260:Ribonucleases; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013891:Thiouridine / Q000032:analysis*
D000926:Anticodon; D010445:Peptide Elongation Factors; D013891:Thiouridine; D006160:Guanosine Triphosphate; D012343:RNA, Transfer; D012260:Ribonucleases; D008239:Lysine; D003488:Cyanogen Bromide