A simple method to remove hemoglobin from antiserum.
Hemoglobin contamination in antiserum occurs frequently in immunohematology. The hemoglobin obscures the ring test for the observation of antigen-antibody reaction. A simple method to remove hemoglobin from antiserum is described in this paper. An appropriate amount of CM-Sephadex was added to antiserum containing hemoglobin and the mixture was packed into a cellulose tube, followed by dialysing against 0.01 M monobasic sodium phosphate solution (pH approx. 6). Equilibrating with stirring, the hemoglobin was completely adsorbed with CM-Sephadex so that hemoglobin-free antiserum was prepared without dilution of the antiserum. This method is useful for immunohematology.
D006405:Hematology / Q000379:methods*; D006454:Hemoglobins; D006801:Humans; D007106:Immune Sera; D007158:Immunologic Techniques
D006454:Hemoglobins; D007106:Immune Sera