Electrophoresis of lymphoid cells. Differences in the cell membrane structure of murine thymocytes, T and B cells revealed by enzyme and formalin treatment.
Low concentrations of protease and trypsin reduced the electrophoretic mobility (EPM) of thymocytes; with higher concentrations it was normal or above. Differences in membrane structure of thymocytes, T and B cells was found as B cells showed no reduction while T cells gave intermediate values. Further the reduction was greater with protease than with trypsin. Formalin fixation increased the EPM of all normal cell types to a similar degree. The EPM of proteolytically treated thymocytes and B cells was increased to a similar level and to a greater degree than neuraminidase-treated thymocytes. Small amounts of sialic acid were detected in the supernatant after proteolytic treatment of thymocytes. Protease reduced the binding of anti-lymphocyte serum, while no definite effect was obtained with trypsin. Neither sublytic doses of phospholipase C nor ribonuclease appeared to change the EPM.
D000818:Animals; D000961:Antilymphocyte Serum; D001402:B-Lymphocytes / Q000648:ultrastructure*; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000032:analysis / Q000187:drug effects / Q000648:ultrastructure; D002465:Cell Movement; D004586:Electrophoresis; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde / Q000494:pharmacology; D008297:Male; D051379:Mice; D010447:Peptide Hydrolases / Q000494:pharmacology; D010740:Phospholipases / Q000494:pharmacology; D012260:Ribonucleases / Q000494:pharmacology; D012794:Sialic Acids / Q000032:analysis; D013601:T-Lymphocytes / Q000648:ultrastructure*; D014357:Trypsin / Q000494:pharmacology
D000961:Antilymphocyte Serum; D012794:Sialic Acids; D005557:Formaldehyde; D010740:Phospholipases; D012260:Ribonucleases; D010447:Peptide Hydrolases; D014357:Trypsin