A simplified procedure for in vitro immunization of dispersed spleen cell cultures.
Dispersed spleen cell suspensions from normal mice were immunized with sheep erythrocytes in flat bottom vial tubes containing a relatively small amount of tissue culture medium fortified with a 'nutrient cocktail'. The magnitude of the number of antibody plaque forming cells appearing in such tube cultures was equivalent or greater than that obtained in Marbrook vessels in the Mishell--Dutton system. Omission of the nutrient cocktail on the day of culture initiation, or the use of round bottom vials, resulted in much lower PFC responses.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D006462:Hemolytic Plaque Technique; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D013154:Spleen / Q000276:immunology*
D003470:Culture Media