[Mechanism of the stimulating effect of protamines on the infectivity of DNA phages].
The interaction of styrin (protamine from sturgeon milt) with E. coli spheroplasts was investigated with the aid of the fluorescent probe--5-dimethylamino-1-naphthalenesulfonyl-chloride (DNS). Not less than 40% of the total (15 mug ml-1) DNA-protamine concentration was bound to spheroplasts. A part of the protein was found in the isolated membranes and inside the cell. There was a correlation between the binding of protein with spheroplasts and its biological action. The dansylation somehow decreased the protein stimulating activity in transfection.
D000818:Animals; D003090:Coliphages / Q000187:drug effects*; D004279:DNA, Viral; D004926:Escherichia coli / Q000187:drug effects; D005399:Fishes; D011479:Protamines / Q000302:isolation & purification / Q000494:pharmacology*; D013104:Spheroplasts / Q000187:drug effects*; D013268:Stimulation, Chemical; D014170:Transformation, Genetic / Q000187:drug effects*
D004279:DNA, Viral; D011479:Protamines
K mekhanizmu stimuliruiushchego deĭstviia protaminov na infektsioznost' DNK fagov