Two simple methods for the quantitative evaluation of platelet adhesion to collagen.
14C serotonine labelled platetel rich plasma (PRP) incubated with fibrillar collagen is either gel filtered through a Sepharose 2B column (Sepharose test) or centrifugated on a 23% Ficoll layer (Ficoll test). In the Sepharose test, the adhesion is quantified by the determination of the per cent yield of free platelets eluted from the column and the release by a quantitative evaluation of the 14C serotonin. In the Ficoll test, the released seroton is measured from the distribution of radioactivity in the different layers (supernatant plasma, platelets adherent to collagen, Ficoll, platelet pellet) of the tube after centrifugation. Both methods are rapid and quite reproducible.
D002250:Carbon Radioisotopes; D002452:Cell Count; D002498:Centrifugation; D002850:Chromatography, Gel; D003094:Collagen / Q000502:physiology*; D005362:Ficoll; D006801:Humans; D008722:Methods; D010973:Platelet Adhesiveness; D012685:Sepharose; D012701:Serotonin / Q000378:metabolism
D002250:Carbon Radioisotopes; D005362:Ficoll; D012701:Serotonin; D003094:Collagen; D012685:Sepharose