Asymmetric distribution of concanavalin A binding sites on yeast plasmalemma and vacuolar membrane.
Isolated vacuoles of Saccharomyces cerevisiae did not bind Concanavalin A (labelled with tritium or with a fluorescent dye) unless the vacuoles were rendered permeable and their inner membrane surface made accessible. Yeast protoplasts, on the other hand, bound large amounts of Concanavalin A on their surface, and the number of binding sites was not increased after a gentle lysis expected to expose also the inner surface of the plasmalemma. It is concluded that both the plasmalemma and the vacuolar membrane carry Concanavalin A binding sites exclusively on the surface opposite to the cytoplasmic matrix.
D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology; D003208:Concanavalin A; D011523:Protoplasts / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D014617:Vacuoles / Q000276:immunology
D003208:Concanavalin A