Nucleotide sequence and in vitro processing of a precursor molecule to Escherichia coli 4.5 S RNA.
A precursor molecule to the stable 4.5 S RNA species of Escherichia coli has been found to accumulate at 42 degrees in a strain thermosensitive for the function of ribonuclease P. The precursor molecule is 130 nucleotides long. Twenty-two extra nucleotides, starting with pppGp, precede the mature sequence at its 5' terminus. At least 1 extra uridine residue can be found at the 3' terminus. The precursor to 4.5 S RNA is cleaved in vitro by RNase P to generate a 5' end identical to that of the mature 4.5 S RNA.
D001483:Base Sequence; D004926:Escherichia coli / Q000378:metabolism*; D006860:Hydrogen Bonding; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000032:analysis; D012329:RNA, Bacterial / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism
D009843:Oligoribonucleotides; D012329:RNA, Bacterial; D012260:Ribonucleases