title: A comparative study of different experimental protocols for mutagenesis assays with the 9-azaguanine resistance system in cultured Chinese hamster cells.
abstract: Both spontaneous and EMS-induced mutant frequencies were determined in cultured cells from V79 Chinese hamsters using three different experimental protocols. After optimal expression time was attained, mutation frequencies only remained constant when a protocol was used in whcih the cell density was maintained below critical values both before and during mutant selection. The identification of such a plateau allows, besides more reliable and reproducible estimates of mutation frequency, reduction in the size of experiments for quantitative evaluation of mutagenicity. Determination of mutation frequencies over a wide range of expression times becomes in fact unnecessary.
mesh_terms: D001375:Azaguanine / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D004351:Drug Resistance; D005821:Genetic Techniques; D009154:Mutation
chemical_list: D001375:Azaguanine