title: Insertion sequence IS2 near the gene for prophage lambda excision.
abstract: In this study we characterize a variant of the lambdacI857S7 prophage, designated lambdabi2cI857S7, which carries a DNA insertion. The insertion sequence is IS2, and it resides in the antipolar orientation II just upstream from the gene for prophage excision (xis) at 61.6%lambda. This bi2 insertion mutant could prove valuable for studies on possible recombination functions of IS2 DNA and of its effect on the lambda integration and excision functions.
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003090:Coliphages; D004279:DNA, Viral; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation
chemical_list: D004279:DNA, Viral