Somatostatin inhibition of insulin and glucagon secretion in rat islet culture: reversal by ionophore A23187.
Both insulin and glucagon release by monolayer cell cultures of newborn rat pancreas were increased when calcium concentration was raised from 1.8 mEq/1 to 7.2 mEq/1. In low calcium medium, the calcium ionophore A23187 (10 mug/ml) enhanced insulin and glucagon secretion. Incubation with somatostatin (1.0 mug/ml), which inhibited hormonal release in a low calcium environment, paradoxically caused augmented insulin and glucagon secretion when A23187 was also present.
D000818:Animals; D002478:Cells, Cultured; D005934:Glucagon / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007476:Ionophores / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D013004:Somatostatin / Q000494:pharmacology*
D007328:Insulin; D007476:Ionophores; D013004:Somatostatin; D005934:Glucagon