Template properties of human DNA-dependent RNA polymerase II.
DNA-dependent RNA polymerase II has been purified from lymphocytes of patients with chronic lymphocytic leukemia (CLL). Form II polymerase and Escherichia coli RNA polymerase have been used to study the transcription of human DNA and chromatin. The hybridization kinetics of the transcripts of DNA and chromatin by the human polymerase are quite different; chromatin transcripts hybridized to DNA at a much slower rate than DNA transcripts, whereas the transcripts of DNA and chromatin by bacterial polymerase have similar hybridization kinetics.
D002499:Centrifugation, Density Gradient; D002843:Chromatin; D002848:Chromatography, DEAE-Cellulose; D004247:DNA; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification; D004926:Escherichia coli; D006801:Humans; D007700:Kinetics; D007945:Leukemia, Lymphoid / Q000201:enzymology; D008214:Lymphocytes; D009693:Nucleic Acid Hybridization; D012319:RNA Polymerase II / Q000302:isolation & purification; D012995:Solubility; D013698:Templates, Genetic; D014158:Transcription, Genetic
D002843:Chromatin; D004247:DNA; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases