title: A non-transmissible variant of RP4 suitable as cloning vehicle for genetic engineering.
mesh_terms: D002876:Chromosomes, Bacterial / Q000032:analysis; D002999:Clone Cells; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis; D005111:Extrachromosomal Inheritance; D014644:Genetic Variation; D008722:Methods; D008970:Molecular Weight; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial