Escherichia coli mutants altered in murein lipoprotein.
Mutants with alterations in the structure, biosynthesis, or assembly of murein lipoprotein were selected by a procedure based on radiation suicide of wild-type organisms by [3H]arginine under conditions where the radioactive arginine was preferentially incorporated into lipoprotein. Further screening for the potential mutants among the survivors of [3H]arginine suicide was carried out by using a sensitive immunodiffusion test, followed by radioactive double-labeling experiments. Three mutants were obtained and partially characterized.
D000409:Alanine / Q000032:analysis; D001120:Arginine / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D003960:Diaminopimelic Acid / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D005944:Glucosamine / Q000032:analysis; D005971:Glutamates / Q000032:analysis; D008074:Lipoproteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D008769:Methylnitronitrosoguanidine; D009112:Muramic Acids / Q000032:analysis; D009153:Mutagens; D009154:Mutation; D010457:Peptidoglycan / Q000032:analysis / Q000096:biosynthesis*
D001426:Bacterial Proteins; D005971:Glutamates; D008074:Lipoproteins; D009112:Muramic Acids; D009153:Mutagens; D010457:Peptidoglycan; D008769:Methylnitronitrosoguanidine; D003960:Diaminopimelic Acid; D001120:Arginine; D005944:Glucosamine; D000409:Alanine