title: Regulation of nitrate reductase at the transcriptional and translational levels in Escherichia coli.
abstract: Nitrate reductase from Escherichia coli is induced by nitrate and derepressed by oxygen removal after a lag phase. Elimination of inducer, shift to aerobic conditions and addition of actinomycin D causes the decline in the rate of its synthesis, which eventually may stop. Kinetic analysis of the sensitivity of the biosynthetic process to oxygen, chloramphenicol, actinomycin D and rifampicin gave results which we interprete as evidence that oxygen (and possibly nitrate) affect simultaneously both the transcriptional and translational processes.
mesh_terms: D002455:Cell Division; D004790:Enzyme Induction / Q000187:drug effects; D004794:Enzyme Repression; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D009565:Nitrate Reductases / Q000096:biosynthesis*; D009566:Nitrates / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D012293:Rifampin / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D009566:Nitrates; D009565:Nitrate Reductases; D010100:Oxygen; D012293:Rifampin