[Use of potassium chloride hypertonic saline solution (3M KC1) for extraction of soluble antigens from Candida albicans].
Soluble antigens have been obtained from Candida albicans cultures of different age treated with hypertonic salt solution (3M KC1). The antigens reached against anti-Candida immune sera in double diffusion agar test, also after adsorption of immune serum on Candida cells. Electron microscopic pictures revealed a marked reduction of the outer layers of 3M KC1 extracted cells.
D000946:Antigens, Fungal / Q000302:isolation & purification*; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000175:diagnosis; D006982:Hypertonic Solutions; D007106:Immune Sera; D008722:Methods; D011189:Potassium Chloride
D000946:Antigens, Fungal; D006982:Hypertonic Solutions; D007106:Immune Sera; D011189:Potassium Chloride
Utilizzazione di soluzione salina ipertonica di cloruro di potassio (3M KC1) per l'estrazione di antigeni solubili da Candia albicans