Studies on the release of gonadotrophins during the superfusion of isolated rat pituitaries in a continuous flow system.
A continuous flow system has been developed to study the endocrine functions of isolated organs. The procedure has been used to investigate the effect of a synthetic gonadotrophin-releasing hormone (Gn-RH) upon the anterior pituitary gland of the adult male rat. A Radiometer blood gas/pH analyser was used to monitor the pH and partial pressures of oxygen and carbon dioxide in the perfusion medium. A series of six experiments with four pituitaries per flask established that the rate of gonadotrophin release under basal conditions was 646 +/- 301 (S.D.) ng LH/ml and 404 +/- 124 (S.D.) ng FSH/ml. The duration and intensity of the response to Gn-RH was assessed by measuring the areas under the curves according to the trapezoidal rule. A significant increase in the release of FSH and LH was obtained by the administration of 250 ng Gn-RH/ml medium, and dose-response curves were produced for up to 10 mug/ml.
D000818:Animals; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D010902:Pituitary Gland / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone