A selective medium (GAMA) for the isolation of somatic cell hybrids from HPRT- and APRT- mutant cells.
A medium is described which can be used for the selection of somatic cell hybrids between HPRT- and APRT- mutant cells. This medium, called GAMA, contains as its relevant constituents the following: the purines guanine and adenine and the purine biosynthetic pathway inhibitors mycophenolic adic, which blocks conversion of adenine ribonucleotides to guanine ribonucleotides, and azaserine, which blocks de novo purine synthesis.
D000228:Adenine Phosphoribosyltransferase / Q000172:deficiency / Q000378:metabolism; D000818:Animals; D001377:Azaserine / Q000494:pharmacology; D002459:Cell Fusion; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D006822:Hybrid Cells / Q000187:drug effects*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency / Q000378:metabolism; D009154:Mutation; D009173:Mycophenolic Acid / Q000494:pharmacology
D003470:Culture Media; D001377:Azaserine; D000228:Adenine Phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase; D009173:Mycophenolic Acid