Transfection of Escherichia coli by Mu DNA.
Infectivity of Mu DNA was demonstrated in Ca+ +-treated Escherichia coli cells that lacked the nucleases Exo V and Endo I. The efficiency of transfection is about 10(-7) per phage equivalent. Infectivity is destroyed by denaturation of Mu DNA, and cannot be restored by renaturation.
D002118:Calcium / Q000494:pharmacology; D003090:Coliphages / Q000187:drug effects / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005838:Genotype; D007700:Kinetics; D010641:Phenotype; D013045:Species Specificity; D014170:Transformation, Genetic; D014779:Virus Replication
D004279:DNA, Viral; D002118:Calcium