title: Human pancreatic islets in culture: effects of supplementing the medium with homologous and heterologous serum.
abstract: Islets of Langerhans, isolated from the human fetal pancreas and cultured in media supplemented with serums from human umbilical cord or adult donors, remained intact, free-floating, and functional for 2 to 5 months. Substitution of fetal calf serum for the human serum usually resulted in attachment of islets, monolayer formation, and relatively early (3 weeks) functional arrest.
mesh_terms: D001769:Blood; D002454:Cell Differentiation; D003470:Culture Media; D046508:Culture Techniques; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000196:embryology / Q000502:physiology; D013045:Species Specificity; D013806:Theophylline / Q000494:pharmacology; D013997:Time Factors
chemical_list: D003470:Culture Media; D007328:Insulin; D013806:Theophylline