title: Absence of an inhibitory effect of oestradiol on LH-RH induced release of LH in vitro caused by inhibition of protein synthesis.
abstract: Pituitary glands of ovariectomized rats were incubated for four hours and the LH released into the medium was measured by radioimmunoassay at one-hour intervals. The effect of a maximally active dose of LH-RH (1000 mg/ml) on pituitary LH release was inhibited by oestradiol (15 ng/ml) added to the media. Although cycloheximide (25 mug/ml) and puromycin (54 mug/ml) in the medium did not affect LH-RH-induced LH release consistently, the inhibitory effect of oestradiol was prevented. It is concluded that this effect of oestradiol is dependent on de novo synthesis of protein.
mesh_terms: D000818:Animals; D002369:Castration; D003513:Cycloheximide / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects*; D011691:Puromycin / Q000494:pharmacology; D051381:Rats
chemical_list: D004965:Estrogen Antagonists; D007987:Gonadotropin-Releasing Hormone; D011691:Puromycin; D004958:Estradiol; D007986:Luteinizing Hormone; D003513:Cycloheximide