Improved radioimmunoassay of urinary estriol.
We report a rapid double-antibody radioimmunoassay for urinary estriol. Advantages over other current methods include: (a) 30-min hydrolysis; (b) total incubation time, 55 min; (c) assay unaffected by urinary glucose; (d) no degradation of estriol evident during hydrolysis; (e) superior (85%) analytical recovery of estriol conjugates; (f) linear standard curve by logit-log extrapolation; (g) good correlation (r = 0.83) with total estrogen determination by a generally accepted colorimetric method; (h) only 20 muL of urine required; and (i) the detection range is 1.9 to 100.5 mg/24-h urine.
D003429:Cross Reactions; D004964:Estriol / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D011863:Radioimmunoassay / Q000379:methods
D004964:Estriol