An improved method for synthesising omega-stearoyldextran for the detection of plaque forming cells specific for the alpha-(1 replaces 6) determinant.
A method for synthesising omega-stearoyldextran for the detection of plaque forming cells specific for the alpha-(1 replaces 6) determinant is described. The omega-stearoyl derivative is obtained by reacting dextran with phenylboronic acid to give a 2,4-phenylboronate, which can be subsequently esterified in a pyridine solution with stearoyl chloride. Four different preparations of omega-stearoyldextran with increasing content of stearoyl were obtained. Chemical and immunochemical properties of these compounds are given.
D000818:Animals; D003911:Dextrans / Q000031:analogs & derivatives*; D006386:Hemagglutination Tests; D006462:Hemolytic Plaque Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D011234:Precipitins / Q000032:analysis; D013228:Stearates / Q000032:analysis / Q000138:chemical synthesis*; D013229:Stearic Acids / Q000138:chemical synthesis*
D003911:Dextrans; D011234:Precipitins; D013228:Stearates; D013229:Stearic Acids