Isolation of kappa-casein-like proteins from milks of various species.
Kappa-Casein-like proteins were isolated from the milks of cow, goat, reindeer, horse, rat, and rabbit. When treated with rennin, all of the isolated kappa-casein components yielded para-kappa-casein-like bands on gel electrophoresis. The rate of cleavage of these components with rennin was determined by measuring material soluble in trichloroacetic acid (macropeptide). The curves were characteristic of a limited, specific attack by rennin on these proteins. The goat and reindeer kappa-caseins were nearly as bovine kappa-casein, but the cleavage of horse, rat, and rabbit kappa-casein-like components was much slower.
D000818:Animals; D002364:Caseins / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D012085:Chymosin / Q000378:metabolism; D005260:Female; D006041:Goats; D006736:Horses; D007700:Kinetics; D046911:Macromolecular Substances; D008892:Milk / Q000032:analysis*; D010446:Peptide Fragments; D011817:Rabbits; D051381:Rats; D012053:Reindeer; D013045:Species Specificity
D002364:Caseins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D012085:Chymosin