The effects of ionophore A23187 on erythrocytes. Relationship of atp and 2,3-diphosphoglycerate to calcium-binding capacity.
The divalent cation ionophore, A23187, was employed as a means to load fresh human erythrocytes with calcium, and the capacity for accumulation was characterized. Erythrocytes exposed to A23187 in calcium-containing media rapidly accumulated calcium in millimolar quantities. The final cellular concentration was dependent upon medium calcium concentration and the size of the cellular organophosphate pool. When ATP and 2,3-diphosphoglycerate contents were depleted or repleted, the cellular calcium content changed proportionally. Calcium loading of fresh erythrocytes produced no discernible change in the cellular concentrations of ATP or 2,3-diphosphoglycerate. Calcium accumulation was also accompanied by loss of cellular potassium and H2O, deterioration of cell filterability, and spheroechinocytic transformation.
D000255:Adenosine Triphosphate / Q000097:blood*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001665:Binding Sites; D001693:Biological Transport, Active; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000097:blood*; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics
D000900:Anti-Bacterial Agents; D004163:Diphosphoglyceric Acids; D000001:Calcimycin; D000255:Adenosine Triphosphate; D002118:Calcium