Attempts to purify a membrane attached chromoid of bacteriophage lambda.
Using methods which proved successful for the isolation of E. coli chromosome in a folded form (the E. coli chromoid), we have attempted to purity the "native" form of bacteriophage gamma chromosome from gamma infected cells. Upon sedimentation of lysates we find that phage DNA separates into two fractions, one of which cosediments with the bacterial chromoid ; the other sediments nearer to the top of gradient. Both fractions probably contain membrane-bound phage DNA, and both support the in vivo synthesis of phage DNA. The heavier fraction contains more closed circular parental DNA molecules than the lighter fraction. Formation of the latter is blocked by certain phage mutations. Being relatively free of bacterial DNA, the lighter fraction is suitable for further analysis.
D002462:Cell Membrane; D002499:Centrifugation, Density Gradient; D002876:Chromosomes, Bacterial; D003090:Coliphages; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification*; D004926:Escherichia coli; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012293:Rifampin / Q000494:pharmacology; D013347:Subcellular Fractions; D014779:Virus Replication / Q000187:drug effects
D004269:DNA, Bacterial; D004279:DNA, Viral; D012293:Rifampin