Whole blood leukocyte culture technique for mammalian cytogenetic analysis.
In the past, the in vitro culture of leukocytes for cytogenetic analysis has required a slightly altered technique for each animal species. This study showed that a simple, yet standardized, in vitro culture system was readily applicable to growth of bovine, canine, equine, feline, ovine and porcine blood leukocytes. The major features of the standardized technique included: 1) the use of whole heparinized blood; 2) a minimum, unfortified culture medium; and 3) a vortex mixer for mixing materials during the hypotonic and fixative stages of the harvesting procedure.
D000818:Animals; D002415:Cats; D002417:Cattle; D002469:Cell Separation; D002478:Cells, Cultured; D003584:Cytological Techniques; D004285:Dogs; D006736:Horses; D007962:Leukocytes / Q000166:cytology*; D012756:Sheep; D013552:Swine