Establishment of a rat hepatoma cell line which has ornithine carbamoyltransferase activity and grows continuously in arginine-deprived medium.
The epithelial cell line, H4-II-E derived from Reuber hepatoma H35 has no significant activity of ornithine carbamoyltransferase (OCT, EC 2.1.3.3) and is not able to grow in arginine-deprived medium. A multi-step selection procedure is described which selects from HR-II-E populations, cells with OCT activity which can grow in arginine-deficient, ornithine-supplemented media.
D000818:Animals; D001120:Arginine / Q000008:administration & dosage*; D002460:Cell Line; D002869:Chromosome Aberrations; D003470:Culture Media; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism*; D051381:Rats
D003470:Culture Media; D001120:Arginine; D009954:Ornithine Carbamoyltransferase