The effect of anti-lymphocyte serum, mitogens and enzymatic treatment on the agglutination and surface charge of lymphoid cells.
Agglutination of thymocytes and B-cells with anti-lymphocyte serum subsequent to treatment of the cells with trypsin or neuraminidase was slightly and similarly increased, independent of the effect on the surface charge shown previously. The enzymatic treatment did not increase capping of antiserum binding sites. Agglutination caused by binding of IgG antibodies of anti-lymphocyte serum was not accompanied by change in the net surface charge. Neither did binding of the mitogens phytohaemagglutinin, concanavalin A or poke-weed mitogen influence the surface charge of the cells.
D000371:Agglutination / Q000187:drug effects; D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000494:pharmacology*; D009439:Neuraminidase / Q000494:pharmacology*; D010740:Phospholipases / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology*
D000961:Antilymphocyte Serum; D007074:Immunoglobulin G; D037102:Lectins; D008934:Mitogens; D003208:Concanavalin A; D008623:Mercaptoethanol; D010740:Phospholipases; D009439:Neuraminidase; D014357:Trypsin