Absence of an inhibitory effect of oestradiol on LH-RH induced release of LH in vitro caused by inhibition of protein synthesis.
Pituitary glands of ovariectomized rats were incubated for four hours and the LH released into the medium was measured by radioimmunoassay at one-hour intervals. The effect of a maximally active dose of LH-RH (1000 mg/ml) on pituitary LH release was inhibited by oestradiol (15 ng/ml) added to the media. Although cycloheximide (25 mug/ml) and puromycin (54 mug/ml) in the medium did not affect LH-RH-induced LH release consistently, the inhibitory effect of oestradiol was prevented. It is concluded that this effect of oestradiol is dependent on de novo synthesis of protein.
D000818:Animals; D002369:Castration; D003513:Cycloheximide / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects*; D011691:Puromycin / Q000494:pharmacology; D051381:Rats
D004965:Estrogen Antagonists; D007987:Gonadotropin-Releasing Hormone; D011691:Puromycin; D004958:Estradiol; D007986:Luteinizing Hormone; D003513:Cycloheximide