Changes in active transport, intracellular adenosine 5'-triphosphate levels, macromolecular syntheses, and glycolysis in an energy-uncoupled mutant of Escherichia coli.
The temperature-sensitive Escherichia coli mutant ecfts metC (Lieberman and Hong, 1974), previously shown to be defective in the coupling of metabolic energy to active transport, is also altered in a wide variety of cellular activities at the nonpermissive temperature. These alterations include a lowering of intracellular adenosine 5'-triphosphate levels, an alteration of glucose metabolism such that large quantities of pyruvate and dihydroxyacetone phosphate are excreted into the medium, excretion of accumulated potassium ions, and a cessation of deoxyribonucleic acid, ribonucleic acid, and phospholipid synthesis. Since these effects closely mimic the action of colicins E1 and K on E. coli cells, the possibility that the ecf gene product is the primary biochemical target for these colicins is discussed.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D009154:Mutation; D010743:Phospholipids / Q000096:biosynthesis; D011188:Potassium / Q000378:metabolism; D012329:RNA, Bacterial / Q000096:biosynthesis*; D013696:Temperature; D013862:Thiogalactosides / Q000378:metabolism*; D013865:Thioglycosides / Q000378:metabolism*
D000596:Amino Acids; D010743:Phospholipids; D012329:RNA, Bacterial; D013862:Thiogalactosides; D013865:Thioglycosides; D000255:Adenosine Triphosphate; D005947:Glucose; D011188:Potassium