title: A gene controlling the synthesis of non specific alkaline phosphatase in Saccharomyces cerevisiae.
abstract: Recessive mutants defective in the formation of non-specific alkaline phosphatase (EC 3.1.3.1) could be selected by staining colonies on a plate with p-nitrophenylphosphate after treatment with chloroform vapour. Since no complementation was observed among the nine mutants so far tested, all the mutations might occur in the same locus, phoH. The non-specific alkaline phosphatase was repressible, although a significant basal level of the enzyme activity was observed in the repressed condition.
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D002850:Chromatography, Gel; D003433:Crosses, Genetic; D004794:Enzyme Repression; D005809:Genes, Regulator; D005838:Genotype; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D000469:Alkaline Phosphatase