Isolation of human eosinophils and their lymphocyte-like rosetting properties.
A method for the isolation of large numbers of almost pure human eosinophils from peripheral blood using hypague-ficoll density gradients, gelatin sedimentation and carbonyl iron is described. Eosinophils were obtained from patients with parasitic infections, allergic reactions, undiagnosed eosinophilias and normal volunteers. Erythrocyte (E) and erythrocyte-antibody-complement (EAC) rosettes were formed by a number of eosinophils.
D002469:Cell Separation / Q000379:methods; D003165:Complement System Proteins; D004804:Eosinophils / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques; D007958:Leukocyte Count
D003165:Complement System Proteins