title: Modulating effects of interferon preparations on an antibody response in vitro.
abstract: The effect of mouse interferon preparations on the primary in vitro antibody response of mouse spleen cells was studied. Concentrations of interferon greater than 8 units per ml significantly inhibited the antibody response while low concentrations of 0-08-0-8 units per ml could be shown to be mildly enhancing. Various treatments which affected the antiviral activity of the interferon preparations reduced the immunosuppressive activity to a similar extent. Interferon acts during the first few hours of a response but the effect is not apparent for at least 50 h. Interferon had no effect when added after 48 h. The kinetic data has been interpreted as demonstrating interferon-sensitive and interferon-resistant components of the in vitro response. The results from investigations of the polyclonal response to lipopolysaccharide support the view that interferon acts mainly on B cells alone, although effects on T-B interactions cannot be excluded.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007166:Immunosuppressive Agents; D007372:Interferons / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008808:Mice, Inbred CBA / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
chemical_list: D007166:Immunosuppressive Agents; D007372:Interferons