title: Mating reaction in Saccharomyces cerevisiae. IX. Regulation of sexual cell agglutinability of a type cells by a sex factor produced by alpha type cells.
abstract: A diffusible sex-specific substance called alpha substance-I (alphaS-I) was isolated from culture filtrate of alpha type strains of the yeast Saccharomyces cerevisiae. The isolated alphaS-I, an oligopeptide, induced sexual cell agglutinability in inducible alpha type strains and enhanced the agglutinability in constitutive a type strains. The induction of sexual agglutinability was detected in 30 min and reached maximum in 90 min, when 0.2 mug/ml of alphaS-I was added to inducible a type cells. The a type-specific factor responsible for sexual cell agglutination, called a type agglutination factor (aAF), was shown to be produced during the induction or the enhancement of agglutinability of a type cells by alphaS-I. The aAF produced in response to alphaS-I was not different in the susceptibility to proteolytic enzymes and disulfide-cleaving agents from those produced constitutively in the absence of alphaS-I.
mesh_terms: D000371:Agglutination; D003513:Cycloheximide / Q000494:pharmacology; D010450:Endopeptidases / Q000494:pharmacology; D005827:Genetics, Microbial; D005854:Germ Cells / Q000502:physiology; D006868:Hydrolysis; D009842:Oligopeptides / Q000494:pharmacology*; D012098:Reproduction; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*; D013438:Sulfhydryl Compounds / Q000494:pharmacology
chemical_list: D009842:Oligopeptides; D013438:Sulfhydryl Compounds; D003513:Cycloheximide; D010450:Endopeptidases