A micromethod for stimulation of chicken lymphocytes in vitro using whole blood.
A simple, rapid and reproducible micromethod for determination of in vitro mitogenic responses of chicken peripheral blood lymphocytes is described. The test utilizes 5 mul volumes of heparinized whole blood, 125I-labelled 5-iodo-2'-deoxyuridine with 5-fluoro-2'-deoxyuridine in place of [3H]thymidine and a multiple cell-culture harvester. The method permits a close follow-up of the mitogenic responses in the chicken.
D000818:Animals; D001769:Blood; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D013696:Temperature