title: Isolation and properties of Escherichia coli mutants which are nonpermissive for the growth of phage lambda.
abstract: By selecting survivors of lambda phage infection, mutants of Escherichia coli K12 that block reproduction cycle of the phage have been isolated. Fourteen of these phage-tolerant mutants (lam mutants) were chosen and characterized biochemically and genetically. It was shown that these mutants were tolerant to infection by all the lambdoid phages, except for few cases, but they were susceptible to infection by a non-lambdoid temperate phage (phi299), P1 or T phages. The mutants can be divided into at least three groups: (1) A mutant (lam 16) strain that seems to block normal penetration of phage DNA: (2) Three mutant (lam 64, lam 67 and lam 71) strains that block an "early" step(s) of phage growth, including phage DNA synthesis: (3) Six mutant (lam 24, lam 25, lam 26, lam 27, lam 646 and lam 6) strains that block normal functioning of the gene E products and produce unusual head structures. Some lambdoid phages and lambda mutants that overcome the interference by the lam mutations have been obtained, and were used as tools for characterizing the host mutations. Two (lam 12 and lam 13) mutant strains and one (lam 1) mutant were inferred as affecting the expression of "late" genes, and early gene, respectively, by this test.
mesh_terms: D003090:Coliphages / Q000254:growth & development*; D004279:DNA, Viral / Q000096:biosynthesis; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism; D005796:Genes; D005838:Genotype; D008242:Lysogeny; D009154:Mutation; D013696:Temperature
chemical_list: D004279:DNA, Viral