Changes in the distribution of intramembranous particles in hen erythrocytes during cell fusion induced by the bivalent-cation ionophore A23187.
Incubation of hen erythrocytes with Ca2+ and the bivalent-cation ionophore A23187 induced slight cell fusion in 1 h at 37 degrees C, and extensive fusion during a subsequent 15 min at 47 degrees C. Redistributions of intramembranous particles were observed, possibly involving interactions between Ca2+ and phospholipids, which are discussed in relation to molecular mechanimss of cell fusion.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002459:Cell Fusion / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D002645:Chickens; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure*; D066298:In Vitro Techniques; D007476:Ionophores; D008854:Microscopy, Electron; D013696:Temperature; D013997:Time Factors
D000900:Anti-Bacterial Agents; D007476:Ionophores; D000001:Calcimycin; D002118:Calcium