title: Kinetic processes in Escherichia coli membranes and cells. A laser photolysis study using derivatives of pyrene.
abstract: Pyrene and several derivatives of pyrene are used to investigate photo-induced kinetic processes in whole cells and membranes extracted from Escherichia coli. A mutant of E. coli was used which, under appropriate growth conditions, produced a complete or incomplete lipopolysaccharide in the outer membrane. The pyrene derivatives used were: pyrene sulfonic acid, pyrene butyric acid and the ester of pyrene butyric acid and 10-hydroxydecanoic acid. The pyrene chromophore was excited by the ultraviolet pulse from a Q switch, frequency-doubled, ruby laser. The lifetimes of the pyrene fluorescence in the presence of the quenchers O2, thallous ion (T1+), I-and CH3NO2 were measured and tabulated as second order rate constants. For the most part the quenching rate constants were much lower than the corresponding values observed in simple nonviscous solution, e.g. ethanol. This is interpreted as being due to the location of the probe within the membrane. The membrane inhibits the movement of the quenchers to the excited state. Cell membranes containing complete lipopolysaccharide showed significantly lower quenching rates for the probes pyrene and pyrene sulfonic acid than cell membranes with incomplete lipopolysaccharide. From an amalysis of the kinetic data it is suggested that pyrene and pyrene sulfonic acid are located near and under lipopolysaccharide and close to membrane proteins. On the other hand, no effect of lipopolysaccharide composition was observed for the probes pyrene butyric acid and pyrene butyroyl decanoic acid. This may suggest that these probes are located primarily in the lipid part of the membrane. A simple model for the outer membrane of E. coli is suggested that accounts for the observed laser-induced kinetic processes.
mesh_terms: D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D007834:Lasers; D008070:Lipopolysaccharides / Q000378:metabolism; D008954:Models, Biological; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011721:Pyrenes / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D011721:Pyrenes