[Application of the immunofluorescent (antibody technique) in clinical immunology I. Technical Considerations (author's transl)].
The main problem of fluorescent antibody techniques is the elimination of "non-specific-staining (NSS)". To assure specificity of the reactions the following points have to be taken into account. The preparation of monospecific antisera, the coupling and purification of fluorescent conjugates to achieve optimal molar fluorescein-protein (F/P)-ratios and of appropriate specificity controls. Furthermore the testing of the optimal method for tissue preparation or the investigation of isolated cells is necessary. The paper shows that there is need for a standardization of the fluorescent antibody technique to achieve comparable results in different laboratories.
D000906:Antibodies / Q000032:analysis; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D007106:Immune Sera; D066298:In Vitro Techniques
D000906:Antibodies; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007106:Immune Sera
Die Anwendung der Immunfluoreszenz (fluoreszierende Antikörpertechnik) in der klinischen Immunologie. I. Erläuterungen zur Methodik