Fluorescent 2'(3')-O-aminoacylnucleosides-acceptor substrates for ribosomal peptidyltransferase+.
2'(3')-O-L-Phenylalanylderivatives of fluorescent 1,N6-ethenoadenosine and 3,N4-ethenocytidine were prepared by chemical synthesis. Both compounds are good acceptor substrates in ribosomal peptidyltransferase reactions. Since these compounds cannot form Watson-Crick base pairs, the results indicate that the terminal aminoacyladenosine unit of AA-tRNA is bound to ribosomal protein on the acceptor site of peptidyltransferase and not to rRNA.
D000217:Acyltransferases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D005456:Fluorescent Dyes; D005815:Genetic Code; D010458:Peptidyl Transferases / Q000378:metabolism*; D012263:Ribonucleosides; D012270:Ribosomes / Q000201:enzymology*
D005456:Fluorescent Dyes; D012263:Ribonucleosides; D000217:Acyltransferases; D010458:Peptidyl Transferases