title: The butanol-soluble proteins of Klebsiella aerogenes.
abstract: Membranes of Klebsiella aerogenes were extracted with acid butanol at pH 4.2 and the solubilized proteins were fractionated by precipitation at 0 degrees C and -20 degrees C. The -20 degrees C precipitate was examined by polyacrylamide gel electrophoresis in sodium dodecyl sulphate. Several enzyme activities associated with polysaccharide biosynthesis have been identified in the crude -20 degrees C precipitate and an attempt was made to separate the enzymes from other protein. The role of certain of these enzymes in a control mechanism for polysaccharide synthesis in K. aerogenes has been postulated.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis*; D000440:Butanols; D002462:Cell Membrane / Q000032:analysis; D007711:Klebsiella pneumoniae / Q000032:analysis* / Q000201:enzymology; D010770:Phosphotransferases / Q000032:analysis* / Q000378:metabolism; D012995:Solubility; D013729:Terpenes / Q000378:metabolism
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D000440:Butanols; D013729:Terpenes; D010770:Phosphotransferases