Microtechnique for simultaneous determination of immobilizing and cytotoxic sperm antibodies. Methodological and clinical studies.
A microtechnique for simultaneous detection of immobilizing and cyctotoxic sperm antibodies was elaborated according to the principles of Terasaki and McClelland. The technique was found reproducible, easy to perform and thus suitable for large scale examinations. The test includes determination of titres for immobilizing and cytotoxic effects and provides at the same time an opportunity to observe both complement-dependent and independent activities. Furthermore, agglutination patterns can easily be recorded. The requirement of only small volumes of test samples makes the test suitable also in testing secretions from the genital tract. In these cases, inclusion of a control for anticomplementary activity of the sample is advisable. In serum from 288 men from infertile couples simultaneous occurence of immobilizing and cytotoxic antibodies was observed in eight cases; all of them also had sperm-agglutinins in serum. In two of these cases, immobilizing and, in one case, cytotoxic activity was revealed in seminal plasma using undiluted samples. Patients with negative findings in serum also revealed negative findings in seminal plasma. Immobilizing and cytotoxic antibodies were not disclosed in serum from 247 women from infertile couples.
D000372:Agglutination Tests; D000373:Agglutinins; D000906:Antibodies / Q000032:analysis*; D002464:Cell Migration Inhibition; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007246:Infertility / Q000276:immunology; D008297:Male; D012661:Semen / Q000276:immunology; D013081:Sperm Motility; D013094:Spermatozoa / Q000276:immunology*; D013997:Time Factors
D000373:Agglutinins; D000906:Antibodies; D003165:Complement System Proteins