Transformation of Escherichia coli by R404 factor DNA and properties of the transformants.
Three r-determinants (Cb Cm Cf) out of eight present in R404 factor were transferred in transformation of Escherichia coli by the plasmid DNA isolated from minicells. The r-determinants acquired in this way formed the replicon tr404-1 and were untransferable by conjugation unless another self-transmissible plasmid was present in recipient. The evidences are presented in support of hypothesis that the transfer events consist in infection of transformant cells with sex factor, recombination between tr404-1 replicon and the sex factor and finally transfer of the new formed structure to the host cells of the sex factor.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003090:Coliphages / Q000254:growth & development; D003227:Conjugation, Genetic; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects*; D005144:F Factor; D008242:Lysogeny; D011815:R Factors; D011995:Recombination, Genetic; D012477:Salmonella enteritidis / Q000187:drug effects; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial