The identification of actin as a major lymphocyte component.
Lymphocytes derived from pig mesenteric lymph node and mouse spleen have been shown to contain a major protein conponent which a) co-migrates on one-dimensional (SDS-PAGE with purified pig muscle actin, and b) possesses the same isoelectric point as muscle actin on two-dimensional gel electrophoresis. Further, limited proteolytic digestion of the lymphocyte actin-comigrating band by S. aureus V8-protease revealed extensive primary sequence hemology with purified muscle actin. Based on the virtual identity of molecular size charge, and primary sequence, we conclude that the actin-comigrating band represents lymphocyte actin. The appearance of lymphocyte actin as a nonspecific "contaminant" of immune precipitates has also been discussed.
D000199:Actins / Q000302:isolation & purification*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes; D008715:Methionine / Q000378:metabolism; D051379:Mice; D010447:Peptide Hydrolases / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D013211:Staphylococcus aureus / Q000201:enzymology; D013552:Swine
D000199:Actins; D008715:Methionine; D010447:Peptide Hydrolases