[Manufacture and possibilities of use of antisera against solubilized human erythrocyte membranes].
Antisera produced in rabbits against Triton X-100 solubilized human erythrocyte ghosts formed several several precipitation lines in immunoelectrophoresis and immunodiffusion tests against the solubilized ghosts. The antisera were used for detecting several membrane components during chromatographic separation. A membrane component was partially isolated using gel filtration on SEPHADEX G-200 (fraction F1/F2). Spectrin (Tektin A) was shown to be the main moiety of these fractions. Associated proteins (haemoglobin, GAPD) could not be detected by the antisera against whole ghost solubilisate.
D000373:Agglutinins; D002462:Cell Membrane / Q000276:immunology*; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D007106:Immune Sera; D007158:Immunologic Techniques; D037102:Lectins; D012995:Solubility
D000373:Agglutinins; D007106:Immune Sera; D037102:Lectins
Erzeugung und Anwendungsmöglichkeiten von Antiseren gegen solubilisierte menschliche Erythrozytenmembranen