title: Studies on ribosomal ribonucleic acid from yeast. III. Secondary structure of 18 and 26S yeast ribosomal RNA's and their complex: circular dichroism and infrared analyses.
abstract: Circular dichroism (CD) and infrared (IR) spectroscopic analyses were performed of 26 and 18S yeast ribosomal RNA's (rRNA) and their specific complex, 30S RNA. The molecular ellipticity coefficients [thota] of 18, 26, and 30S rRNA's were 2.72, 2.63, and 2.65X10(4) degree-cm2/decimole at 264 nm, respectively. The base-pairing contents of 18, 26, and 30S rna's determined by iC), and 70% (32% AU, 38% GC), respectively. These results suggest that 18 and 26S rRNA have very similar secondary structures, and that 30S rna may have a slightly higher base-pairing content than the estimated sum of those of 18 and 26S rRNA's. The biological significance of this phenomenon is discussed in this report.
mesh_terms: D001665:Binding Sites; D002942:Circular Dichroism; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D013055:Spectrophotometry, Infrared
chemical_list: D012335:RNA, Ribosomal