[Modification of the local hemolysis method for the detection of antibody producing cells producing antibody to virus antigens].
A modification of Jerne and Nordin's method of local hemolysis for detection of antiviral antibody-synthesizing cells is described. CrCl3 was used as the coupling agent. This method is simple enough but it requires either highly purified virus antigen or viruses propagated in a heterologous culture.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000921:Antibody-Producing Cells / Q000276:immunology*; D000956:Antigens, Viral; D002857:Chromium; D006462:Hemolytic Plaque Technique / Q000379:methods*; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D013154:Spleen / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D002857:Chromium
Modifikatsiia metoda lokal'nogo gemoliza dlia obnaruzheniia kletok, sinteziruiushchikh antitela k virusnym antigenam