Identification of genes for elongation factor Ts and ribosomal protein S2 in E. coli.
The structural gene for elongation factor EF-TS (tsf) and that for ribosomal protein S2 (rpsB) have been identified in E. coli. Both genes are carried by lambda transducing phages that have been isolated as dapD+polC+ transducing phages. Synthesis of both S2 and EF-Ts was demonstrated in ultraviolet light-irradiated E. coli cells infected with these phages. Experiments were also done using other transducing phages that carry dapD+ but not polC+. The data indicate that both the tsf and rpsB genes map near dapD at about 4 min on the E. coli genetic map. This location is different from the two chromosomal locations, the str-spc region and the rif region, where many ribosomal protein genes, the genes for RNA polymerase components, as well as other elongation factor genes (fus, tufA, and tufB) are located.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002874:Chromosome Mapping; D003090:Coliphages; D004926:Escherichia coli / Q000378:metabolism*; D005796:Genes; D009154:Mutation; D012269:Ribosomal Proteins / Q000096:biosynthesis*; D014161:Transduction, Genetic
D001426:Bacterial Proteins; D012269:Ribosomal Proteins