title: Catalysis of the peptide bond formation by 50 S subunits of E. coli ribosomes with N-(formyl) methionine ester of adenylic acid as peptide donor.
abstract: 50 S subunits of E. coli ribosomes catalyze the reaction of the 2'(3')-N-(formyl) methionine ester of adenosine 5'-phosphate and Phe-tRNA resulting in peptide bond synthesis. Cytidine 5'-phosphate stimulates this process on 50 S ribosomal subunits as well as on intact ribosomes. The obtained data show that the areas of the peptidyltransferase donor site which binds the 3'-terminal fragment of peptidyl-tRNA possess completely formed structures on 50 S ribosomal subunits.
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D000249:Adenosine Monophosphate; D002458:Cell Fractionation; D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008034:Lincomycin / Q000494:pharmacology; D008715:Methionine / Q000031:analogs & derivatives; D010458:Peptidyl Transferases / Q000378:metabolism*; D010649:Phenylalanine; D012343:RNA, Transfer / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D046249:Transfer RNA Aminoacylation
chemical_list: D012269:Ribosomal Proteins; D000249:Adenosine Monophosphate; D010649:Phenylalanine; D002701:Chloramphenicol; D012343:RNA, Transfer; D008715:Methionine; D008034:Lincomycin; D000217:Acyltransferases; D010458:Peptidyl Transferases