Evaluation of antibiotic efficacy using electron microscopy: morphological effects of guanylureido cephalosporin, BL-P1654, and carbenicillin on Escherichia coli.
Early response of Escherichia coli to minimal inhibitory concentrations of 112883, BL-P1654, and carbenicillin was determined by [(14)C]leucine uptake and scanning electron microscopy morphology studies. [(14)C]leucine uptake was inhibited later by carbenicillin than by BL-P1654 or 112883. Cellular swelling at 30 min, septal region swelling, and lysis were the progressive morphological changes with BL-P1654 and 112883. Carbenicillin-treated cells showed septal region swelling, beaded chains, and lysis.
D002228:Carbenicillin / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D007930:Leucine / Q000378:metabolism; D008855:Microscopy, Electron, Scanning
D002511:Cephalosporins; D003470:Culture Media; D002228:Carbenicillin; D007930:Leucine