A restriction cleavage map of phiX 174 DNA by pulse-chase labelling using E. coli DNA polymerase.
A pulse chase labelling technique using E. coli DNA polymerase I has been used to determine a number of restriction endonuclease cleavage maps of ØX 174 DNA. In addition to verifying the accuracy of the method by confirming some previously published maps, the Hha I and Alu I maps have also been constructed.Images
D002874:Chromosome Mapping; D003090:Coliphages / Q000032:analysis*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D006193:Haemophilus influenzae / Q000201:enzymology
D004279:DNA, Viral; D004254:DNA Nucleotidyltransferases; D004262:DNA Restriction Enzymes