In vitro spheroplast and L-form induction within the pathogenic nocardiae.
Six strains of Nocardia asteroides, two strains of N. caviae, and two strains of N. braziliensis were grown in medium supplementted with glycine, lysozyme, D-cycloserine, glycine plus lysozyme, and glycine plus D-cycloserine. It was shown that three strains of N. asteroides, and two strains of N. caviae, readily formed spheroplasts and/or protoplasts when grown in the presence of glycine plus either lysozyme or D-cycloserine. This process was studied by both phase contrast microscopy and electron microscopy. The induced cultures were then plated on hypertonic medium for the isolation of L-forms. It was shown that the organisms differed greatly in their ability to produce spheroplasts and subsequently grew as L-forms or transitional-phase variants.
D003470:Culture Media; D003523:Cycloserine / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology; D007740:L Forms / Q000302:isolation & purification / Q000648:ultrastructure*; D009113:Muramidase / Q000494:pharmacology; D009615:Nocardia / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure*; D009616:Nocardia asteroides / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure; D013104:Spheroplasts / Q000302:isolation & purification / Q000648:ultrastructure*
D003470:Culture Media; D003523:Cycloserine; D009113:Muramidase; D005998:Glycine