Convenient method for detecting 14CO2 in multiple samples: application to rapid screening for mutants.
A procedure is presented for the rapid screening of bacterial colonies to detect mutants unable to produce 14CO2 from a labeled precursor. The method is especially useful for mass screening for mutants that cannot be easily detected by their phenotypic characteristics.
D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism; D001431:Bacteriological Techniques; D002245:Carbon Dioxide / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D009154:Mutation; D013696:Temperature
D002245:Carbon Dioxide; D012437:Adenosylmethionine Decarboxylase