title: Genetic activity of the antimicrobial food additives AF-2 and H-193 in Saccharomyces cerevisiae.
abstract: The genetic activity of the antimicrobial food additives AF-2 and H-193 has been investigated in Saccharomyces cerevisiae. The strains chosen for the present studies were D5 for the induction of mitotic recombinational events and XV185-14C for the induction of reversion of the mutants lys1-1, his1-7 and homs3-7. When three concentrations (25, 50 and 100 mug/ml) of AF-2 were used in the reversion system of strain XV185-14C, there was an increase in the frequency of hom+ and his+ revertants as a function of incubation time, while the lysine mutant exhibited a very low frequency of induced reversion. When AF-2 and H-193 were compared at the same concentration and exposure time, AF-2 exhibited a higher genetic activity in both systems than H-193. However, H-193 was genetically more active in inducing revertants than AF-2, when the comparison was made at the same survival level. Cells of both haploid and diploid strains were found to be more sensitive to inactivation by AF-2 than by H-193 It should be pointed out that the solubility of H-193 was lower (about 4 mug/ml saturation) than the solubility of AF-2 (120 mug/ml saturation). The haploid strain was more sensitive to both compounds than the diploid strain.
mesh_terms: D000178:Acrylamides / Q000494:pharmacology*; D001549:Benzamides / Q000494:pharmacology*; D004171:Diploidy; D005503:Food Additives / Q000494:pharmacology*; D005787:Gene Frequency; D006238:Haploidy; D008938:Mitosis; D009154:Mutation; D009581:Nitrofurans / Q000494:pharmacology*; D011995:Recombination, Genetic / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects*
chemical_list: D000178:Acrylamides; D001549:Benzamides; D005503:Food Additives; D009581:Nitrofurans; C012111:H 193