[Interactions between various transition elements in their effect on pepsin activity].
Kinetic experiments were made with pepsin in the presence of Cu2+ ions and additional Fe2+, Ni2+ or Zn3+ ions as sulphates. As a parameter of the pepsin activity the turnover-rate curves were determined with haemoglobin as a substrate. An important activation of the pepsin was obtained by Cu2+ additions of 5-10(-4) mole/1. When Cu2+ and Fe2+ ions were added to the reaction mixture at the same time, an additive effect of both metal cations was observed. A competitive effect of both metal cations was found in the combination of Cu2+/Ni2+ ions. Zn2+ addition did not influence the activation of pepsin caused by Cu2+ ions.
D000818:Animals; D003300:Copper / Q000494:pharmacology; D004347:Drug Interactions; D006454:Hemoglobins / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology; D007501:Iron / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D009532:Nickel / Q000494:pharmacology; D010434:Pepsin A / Q000378:metabolism*; D013552:Swine; D015032:Zinc / Q000494:pharmacology
D006454:Hemoglobins; D008670:Metals; D003300:Copper; D009532:Nickel; D007501:Iron; D010434:Pepsin A; D015032:Zinc
Interaktionen zwischen einigen Ubergangselementen in ihrem Einflubeta auf die Pepsinaktivität