Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock.
Ionic shock treatment in the presence of 10% glycerol is an efficient and selective method for extracting cell surface components from Raji cells and effecting the solubilization of up to 22 polypeptides. The majority of these shock-released polypeptides are accessible to lactoperoxidase radioiodination. Sera from rabbits immunized against these soluble extracts are reactive against Raji cell surface as indicated by indirect membrane immunofluorescence and agglutination assays.
D000372:Agglutination Tests; D002462:Cell Membrane / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007784:Lactoperoxidase; D008214:Lymphocytes / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D013347:Subcellular Fractions / Q000032:analysis
D010455:Peptides; D007784:Lactoperoxidase