Scanning electron microscopy of postoperative M-K and moist chamber-stored corneas.
In the present study, using the in vivo test situation which all corneal donor material must meet, we performed penetrating keratoplastics utilizing corneal tissue stored for five days in either M-K medium or conventional moist chambers. Cat corneas stored for five days in M-K medium had a higher incidence of postoperative graft clarity along with an intact endothelial cell layer as determined by scanning electron microscopy than corneas stored in a conventional moist chamber. The endothelium of corneas immediately after moist chamber storage revealed minimal surface alteration which contrasted to the more severely stressed endothelium immediately after M-K storage. However, the M-K stored endothelium survived the trauma of penetrating keratoplasty much better than cells stored in moist chambers.
D000818:Animals; D002415:Cats; D003315:Cornea / Q000648:ultrastructure; D016039:Corneal Transplantation; D004727:Endothelium / Q000648:ultrastructure; D012996:Solutions; D014021:Tissue Preservation / Q000379:methods*; D014184:Transplantation, Homologous
D012996:Solutions