Preparation and characterization of antithymocyte serum and globulin without stem cell activity.
Immunosuppressive antimouse and antihuman thymocyte sera and antihuman T lymphocyte sera, raised in rabbits, inhibited colony formation in vitro by bone marrow stem cells. Absorption of the antisera with fetal liver, or isolation of the gamma-globulin from the antisera, removed the inhibitory effect on bone marrow stem cells but left the anti T lymphocyte activity unimpaired. Absorption with cultured lymphoblasts (B cell lines) failed to remove the affinity of the globulins for B lymphocytes.
D000371:Agglutination; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification*; D000961:Antilymphocyte Serum / Q000302:isolation & purification* / Q000494:pharmacology; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells; D006801:Humans; D007104:Immune Adherence Reaction; D051379:Mice; D010587:Phagocytosis; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000961:Antilymphocyte Serum