Ways of collagen separation in pathologically altered tissue.
A system of chromatographic methods using two successive DEAE-cellulose chromatographic steps and two successive separations on Bio-Gel A-1.5 m has been worked out for the separation of individual collagen types. The success of the procedure is based on the preliminary removal of proteoglycans during the first DEAE-cellulose run. Alternatively it is possible to replace chromatographic steps, following the removal of proteoglycans, with fraction precipitation.
D002847:Chromatography, Agarose; D002848:Chromatography, DEAE-Cellulose; D003094:Collagen / Q000302:isolation & purification*; D006801:Humans; D007668:Kidney / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D011704:Pyelonephritis / Q000378:metabolism
D011509:Proteoglycans; D003094:Collagen