Chromatographic separation of human salivary peroxidases.
A series of rapid and simple chromatographic purification procedures for peroxidase-like enzymes occurring in the human oral cavity is presented. Samples of whole saliva, parotid saliva, gingival exudate and various bacterial preparations contain peroxidases which were purified using molecular exclusion and ion exchange chromatography, and isoelectric focusing. Salivary lactoperoxidase can be easily separated from bacterial and leucocyte peroxidase activity by the methods presented.
D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003773:Dental Plaque / Q000201:enzymology; D005883:Gingival Crevicular Fluid / Q000201:enzymology; D006801:Humans; D007202:Indicators and Reagents; D007525:Isoelectric Focusing; D010544:Peroxidases / Q000097:blood / Q000302:isolation & purification*; D012463:Saliva / Q000201:enzymology*; D013048:Specimen Handling
D007202:Indicators and Reagents; D010544:Peroxidases