title: The use of 1-fluoro-2,4-dinitrobenzene as an affinity label for the antigen receptor of delayed hypersensitivity.
abstract: The passive transfer of delayed contact sensitivity to dinitrophenyl can be specifically inhibited by brief treatment of lymph node cells (LNC) from sensitized guinea-pigs with 1-fluoro-2,4-dinitrobenzene (DNFB). Analysis of KC1 extracts of LNC previously treated with (3H)DNFB, using polyacrylamide gel electrophoresis, revealed a peak of radioactivity present in both sensitive and normal cell extracts as well as a peak found only in sensitive cell extracts. Cell-free extracts appeared capable of transmitting delayed-onset skin reactivity to non-sensitized recipients.
mesh_terms: D000345:Affinity Labels; D000818:Animals; D001666:Binding Sites, Antibody; D002474:Cell-Free System; D004136:Dinitrobenzenes / Q000276:immunology*; D004139:Dinitrofluorobenzene; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007111:Immunity, Cellular; D007116:Immunization, Passive; D007158:Immunologic Techniques; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D009578:Nitrobenzenes / Q000276:immunology*; D012882:Skin Tests
chemical_list: D000345:Affinity Labels; D004136:Dinitrobenzenes; D009578:Nitrobenzenes; D004139:Dinitrofluorobenzene