[Colicin El synthesis in a cell-free system of paired transcription--translation].
Proteins were synthesized in the cell-free coupled transcription-translation systen (S30) under the control of the DNA-templates of plasmid ColEl, phages T2 and Sd. The most active templates were phage T2 DNA and closed plasmid DNA. Plasmid DNA induced synthesis of 8 individual proteins, one of which was a biologically active antibiotic of protein nature--colicin. The titre of colicin synthesized in such a cell-free system reached 1024 U/ml, this being about 100 times greater than the titre of this antibiotic in the colicinogenic bacteria cultures.
D002474:Cell-Free System; D003087:Colicins / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D010957:Plasmids; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D003087:Colicins; D004269:DNA, Bacterial
Sintez kolitsina El v beskletochnoĭ sisteme sparennoĭ transkriptsii--transliatsii