[The influence of bacterial genes recB, recA, exr and polA on T4 phage DNA transfection of lysozyme spheroplasts of Escherichia coli].
The transfection efficiency of T4 phage DNA of Escherichia coli spheroplasts, carrying mutations in four genes, involved in recombination and reparation (recB, recA, exr, polA), is studied. RecB- and recA- mutations increased the phage yield during transfection, and exr- and polA- mutations decreased it. RecA- mutation is suggested to influence on spheroplasts membrane state, resulting in more efficient replication of transfecting phage DNA.
D003090:Coliphages; D004926:Escherichia coli; D005796:Genes; D009154:Mutation; D013045:Species Specificity; D013104:Spheroplasts; D014779:Virus Replication
Vliianie bakterial'nykh genov recB, recA, exr i polA na éffektivnost' transfektsii lizotsimnykh sferoplastov Escherichia coli DNK faga T4