Release of migration inhibitory factor from mouse T and B cells activated by insoluble phytomitogens.
The production of macrophage migration inhibitory factor (MIF) was studied with mouse B spleen cells and with the low density subpopulation of thymus cells (T cells) stimulated by phytomitogens. MIF was released from mouse B cells when stimulated by Sepharose-PWM but was not released by Sepharose-Con A. MIF was produced by the low density subpopulation of thymus cells stimulated by Sepharose-Con A. MIF from B cells could not be distinguished from that of the low density subpopulation of thymus cells by gel filtration on Sephadex G-100 column. The molecular size of both MIF was calculated to be 45,000-55,000 dalton.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology*; D008263:Macrophage Migration-Inhibitory Factors / Q000378:metabolism*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D037102:Lectins; D008263:Macrophage Migration-Inhibitory Factors; D003208:Concanavalin A