Arangement of transfer-RNA -genes in yeast.
The redundancy and the arrangement of the genes for specific transfer ribonucleic acids in yeast were studied by the hybridization techniques developed by Birnstiel et al., e.g.[1]. The redundancy was found to be in the order of 10 genes for tRNA1Met, tRNA3Met, tRNA2Ser, and tRNA-Pro. High molecular weight yeast DNA was fractionated by density gradient centrifugation in cesium chloride and the [32p]tRNAs were hybridized to the single fractions. The results together with earlier findings [2] suggest that the cistrons for these tRNAs are arranged in tandem interspersed by 6 to 10 times longer segments of spacer DNA which varies in (G+C) content for the different tRNA species.
D004247:DNA; D005796:Genes; D008715:Methionine; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000032:analysis; D011392:Proline; D012343:RNA, Transfer; D012441:Saccharomyces cerevisiae; D012694:Serine
D009711:Nucleotides; D012694:Serine; D004247:DNA; D012343:RNA, Transfer; D011392:Proline; D008715:Methionine