New types of streptomycin-resistant mutants of Escherichia coli.
Mutagenesis of Escherichia coli K12 cells with ethyl methanesulfonate and selection of streptomycin-resistant mutants after a long delay for phenotypic expression allowed us to isolate new types of streptomycin-resistant ribosomes. Misreading patterns of the ribosomes in the presence of streptomycin revealed that most of the streptomycin-resistant mutants isolated under these conditions differed from the four classical types of streptomycin-resistant mutants studied and characterized by Strigini, P. and Gorini, L. (1970) J. Mol. Biol. 47, 517-530.
D003090:Coliphages / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D009154:Mutation; D010641:Phenotype; D014176:Protein Biosynthesis / Q000187:drug effects; D012270:Ribosomes / Q000378:metabolism; D013045:Species Specificity; D013307:Streptomycin / Q000494:pharmacology*; D014161:Transduction, Genetic
D005020:Ethyl Methanesulfonate; D013307:Streptomycin