title: The application of immunofluorescence to the study of renal disease. I. Influences of various conditions of specimens and conjugates on the fluorescent patterns of kidney.
abstract: Some basic problems in immunofluorescence were reexamined, limiting the object to kidneys. As to thickness of sections, 4 mum was demonstrated to be the most reasonable also to kidney specimens. Treatment of PBS for a short time before staining was recommended not only for cleanup effect but also for the possibility to disclose covered antigenic determinants. It was also established that fluorescent antibody with adequate F/P molar ratio, as well as with strict specificity, should be used, in order to prevent "false positives".
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002415:Cats; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique / Q000379:methods*; D007668:Kidney / Q000276:immunology*; D007678:Kidney Glomerulus / Q000276:immunology; D051379:Mice