Comparative kinetics of arginine and lysine transport by epimastigotes and trypomastigotes from two strains of Trypanosoma cruzi.
Three-day-old cultures of Y and MR strains of Trypanosoma cruzi ahd a higher rate of lysine and arginine uptake than 10-day cultures. Amino acid uptake by cells of the MR strain was consistently higher than that of the Y strain. Flagellates separated on DEAE-cellulose columns have normal structure, motility, and infectivity; they have the higher rates of lysine and arginine uptake than the original 3- and 10-day cultures. In addition passage through DEAE-cellulose columns modified the kinetic behavior of amino acid transport systems in the flagellate membranes.
D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001693:Biological Transport, Active; D007700:Kinetics; D008239:Lysine / Q000378:metabolism*; D051379:Mice; D014349:Trypanosoma cruzi / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*
D001120:Arginine; D008239:Lysine