Release of hypoxanthine from and enzyme depletion in rat heart cell cultures deprived of oxygen and metabolic substrates.
In a monolayer cultures of neonatal rat heart cells deprived of oxygen and metabolic substrates we measured (1) the release of hypoxanthine, a product of ATP catabolism, and (2) enzyme depletion with creatine phosphokinase (CPK) and alpha-hydroxybutyrate dehydrogenase (alpha-HBDH). Taking samples each hour up to 7 h of anoxia we were able to demonstrate that hypoxanthine release precedes cellular enzyme depletion. The release rate of hypoxanthine is maximal in the second hour of anoxia and the depletion rate of CPK and alpha-HBDH is maximal in the fourth hour. These results suggest that for early diagnosis of damage to heart cells due to deprivation of oxygen and metabolic substrates the measurement of hypoxanthine release is preferred to that of enzyme release.
D000818:Animals; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D007042:Hypoxanthines / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009584:Nitrogen / Q000494:pharmacology; D051381:Rats
D007042:Hypoxanthines; D006884:Hydroxybutyrate Dehydrogenase; D003402:Creatine Kinase; D005947:Glucose; D009584:Nitrogen