title: A transducing bacteriophage lambda carrying the structural gene for elongation factor Ts.
abstract: A specialized transducing bacteriophage lambdadpolCdap D-9 has been isolated that carries the structural gene for EF-Ts1 (tsf). The presence of EF-Ts among the proteins synthesized under the direction of this phage in UVL-inactivated cells has been detected by two-dimensional gel electrophoresis and has been verified by antibody precipitation. In an induced lysogen of this phage the relative rate of synthesis of EF-Ts is increased 4-fold. Evidence is presented which suggest that the structural genes for ribosomal protein S2 (rpsB) and RNA polymerase sigma factor (sit) also lies on lambdadpolCdap D-9.
mesh_terms: D003090:Coliphages / Q000302:isolation & purification; D004267:DNA Viruses; D012321:DNA-Directed RNA Polymerases; D004586:Electrophoresis; D004926:Escherichia coli / Q000528:radiation effects; D005796:Genes; D010445:Peptide Elongation Factors; D012329:RNA, Bacterial; D012269:Ribosomal Proteins; D014161:Transduction, Genetic; D014466:Ultraviolet Rays; D014764:Viral Proteins
chemical_list: D010445:Peptide Elongation Factors; D012329:RNA, Bacterial; D012269:Ribosomal Proteins; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases