Cholesterol biosynthesis in a variety of cultured cells. Lack of correlation between synthesis and activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase caused by dexamethasone.
In a variety of cell lines grown in the presence of 10(-6) M dexamethasone, the incorporation of acetate into cellular cholesterol was partially suppressed. However, the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (mevalonate:NADP+ oxidoreductase (CoA-acylating), EC 1.1.1.34) was either significantly elevated or remained unchanged providing evidence that under certain conditions 3-hydroxy-3-methylglutaryl coenzyme A reductase activity is not a valid measure of cholesterogenesis.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis*; D003907:Dexamethasone / Q000494:pharmacology*; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D051381:Rats
D000085:Acetates; D003907:Dexamethasone; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases