Semiquantitative determination of soluble fibrin monomer complexes by chromatography and serial-dilution protamine sulfate test.
Plasmas were prepared from blood obtained from rabbits and from healthy individuals and patients with thrombotic disorders. Gel filtration (Sepharose-4B) chromatography and the serial-dilution protamine sulfate (SDPS) test were performed on each sample. For healthy individuals and rabbits, a narrow fibrinogen peak was obtained on chromatogram and was associted with a negative SDPS test. After administration of thrombin or endotoxin to rabbits and in plasmas from patients with intravascular coagulation, major shifts to earlier effluent volumes occurred. These higher-molecular-weight derivatives corresponded to soluble fibrin monomer complexes (FM). Positive SDPS tests were found in these plasmas. There was a highly significant correlation between percentage area occupied by FM on chromatogram and SDPS test titer. It is concluded that the SDPS test is a reliable semiquantitative measure of FM that can be used clinically as well as in experimental animal models.
D000818:Animals; D002850:Chromatography, Gel; D005340:Fibrinogen / Q000032:analysis; D006652:Histological Techniques; D006801:Humans; D008970:Molecular Weight; D011479:Protamines; D011817:Rabbits; D012995:Solubility; D013210:Staphylococcus
D011479:Protamines; D005340:Fibrinogen