The detection of viruses by enzyme-linked immunosorbent assay (ELISA).
The use of enzyme-linked antibodies for the detection of two morphologically different plant viruses is described. The technique is extremely sensitive, enabling assay of the viruses at concentrations as low as 10 to 100 ng/ml both in purified preparations and in crude plant extracts.
D000469:Alkaline Phosphatase / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques; D010942:Plant Viruses / Q000302:isolation & purification*
D000469:Alkaline Phosphatase