Evidence for a conformational change in tRNAPhe upon aminoacylation.
A conformational difference between the structure of tRNAPhe and Cbz-Phe-tRNAPhe from Escherichia coli was detected using the spin label method. A comparison of the respective rotational correlation time (tau c) values of three differently located spin labels, indicates that upon aminoacylation of tRNAPhe, the 4-thiouridine residue region and the miniloop region become more flexible, while the environment of the anticodon loop is not affected. These observations suggest that the main difference in structure between charged and uncharged tRNA resides in the release and exposure of the TpsiC loop for eventual binding to the ribosomes.
D004926:Escherichia coli / Q000378:metabolism*; D010649:Phenylalanine / Q000031:analogs & derivatives; D012343:RNA, Transfer / Q000378:metabolism*; D046249:Transfer RNA Aminoacylation
D010649:Phenylalanine; D012343:RNA, Transfer