Passive hemolysis inhibition test for quantitation of serologically active polysaccharides and oligosaccharides in micro amounts.
An improved quantitative micromethod is described for the assay of antigenically active bacterial lipopolysaccharides and their immunodeterminant oligosaccharides. The method which is capable of measuring antigens in nanogram quantities yields the same sensitivity as the microcomplement fixation without the limitations of this method. The use of the method was demonstrated on Shigella sonnei phase 1 and phase ii lipopolysaccharide antigens and immunodeterminants.
D000818:Animals; D003168:Complement Fixation Tests; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D006461:Hemolysis; D046911:Macromolecular Substances; D008832:Microchemistry; D009844:Oligosaccharides / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D012756:Sheep; D012764:Shigella sonnei / Q000276:immunology
D046911:Macromolecular Substances; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial