title: Selection of auxotrophic mutants in Saccharomyces cerebisiae by a snail enzyme digestion method.
abstract: Yeast cells in the stationary phase of growth are relatively resistant to snail enzyme digestion. This resistance was shown to decrease abruptly in the course of only 3-5 duplications, when stationary cells were allowed to grow in a fresh medium. The selective digestion of growing prototrophs from a mutagenized culture in minimal medium by snail enzyme was applied to increase the proportion of auxotrophs which remained relatively resistant.
mesh_terms: D002455:Cell Division; D004798:Enzymes / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008722:Methods; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D012908:Snails / Q000201:enzymology
chemical_list: D004798:Enzymes; D005947:Glucose; D009584:Nitrogen