DNA synthesis in vitro dependent upon phiX174 replicative form I DNA.
Extracts of Escherichia coli strains infected with bacteriophage phiX174 catalyze DNA synthesis dependent on double-stranded, circular phiX174 replicative form I (phiX RFI) by a semiconservative process. The reaction required Mg++, ATP, all four dNTP, and exogenous phiX RFI DNA as template and yielded phiX RFI and phiX RFII. The reaction was inhibited by nalidixic acid and novobiocin but not by rifampicin. DNA synthesis required the phiX174 gene A product and E. coli gene products dnaB, dnaC(D), dnaG, and rep.
D001426:Bacterial Proteins / Q000378:metabolism; D002474:Cell-Free System; D003090:Coliphages / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli; D005796:Genes; D007700:Kinetics; D009268:Nalidixic Acid / Q000494:pharmacology; D009675:Novobiocin / Q000494:pharmacology; D009690:Nucleic Acid Conformation
D001426:Bacterial Proteins; D004279:DNA, Viral; D009675:Novobiocin; D009268:Nalidixic Acid