title: Improved resolution in cathodoluminescent microscopy of biological material.
abstract: This paper continues work reported in an earlier paper on modification of a Cambridge Stereoscan Mk IIA to improve the quality of cathodoluminescent micrographs of biological material. In the work presently described the microscope gun has been offset laterally by 2 mm, to prevent light from the filament passing down through the column to the specimen chamber. The electron beam is brought onto the column axis by deflection coils. This modification effectively eliminates background light in the chamber, and a full parabolic mirror is fitted to maximize light collection. Results for yeasts and wheat seed sections are described.
mesh_terms: D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*; D012568:Schizosaccharomyces / Q000648:ultrastructure; D012639:Seeds / Q000648:ultrastructure; D014908:Triticum / Q000648:ultrastructure