The biosynthesis of the thiazole moiety of thiamine in Salmonella Typhimurium.
The mechanism of biosynthesis of 4-methyl-5-beta-hydroxyethyl thiazole, the thiazole moiety of thiamine was studied in Salmonella typhimurium. Using the adenosine derepression technique the incorporation of various 14C-labeled precursors was determined. We found that;e1Me-14C]methionine, [2-14C]methionine, [U-14C]alanine, and [2-14C]glycine were not incorporated whereas [2-14C]tyrosine was incorporated. Degradation of the 4-methyl-5-beta-hydroxyethyl thiazole obtained after [2-14C]tyrosine incorporation revealed that all of the activity was located on carbon-2. These findings are discussed and compared with previous findings concerning 4-methyl-5-beta-hydroxyethyl thiazole biosynthesis.
D000409:Alanine / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism*; D013831:Thiamine / Q000096:biosynthesis*; D013844:Thiazoles / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
D013844:Thiazoles; D014443:Tyrosine; D008715:Methionine; D000409:Alanine; D005998:Glycine; D013831:Thiamine