title: DNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro.
abstract: The in vitro synthesis of ribosomal protein L12 has been obtained in a coupled system with DNA extracted from the transducing phage lambdarifd18 as template. In addition, a second protein (molecular weight of 16,000) with immunological, chemical, and ribosome-binding characteristics similar to L12 is formed in this in vitro system. The synthesis of both proteins is depressed by one-half when guanosine-5'-diphosphate-3'-diphosphate is added to the reaction mixture.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis*; D002474:Cell-Free System; D003090:Coliphages; D004269:DNA, Bacterial / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli; D006150:Guanine Nucleotides / Q000494:pharmacology; D007700:Kinetics; D008970:Molecular Weight; D011485:Protein Binding; D012269:Ribosomal Proteins / Q000096:biosynthesis*; D012270:Ribosomes / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004279:DNA, Viral; D006150:Guanine Nucleotides; D012269:Ribosomal Proteins