Evaluation of a DNA polymerase-deficient mutant of E. coli for the rapid detection of carcinogens.
Differential growth inhibition of two E. coli cultures was evaluated as a rapid screening technique for chemical carcinogens. Of the carcinogens tested, only "direct acting" carcinogens produced positive results. Furthermore, this test is not a quantitative assay in that neither was a dose--response relationship seen nor did potent carcinogens necessarily show a greater response than weaker carcinogens.
D002273:Carcinogens / Q000032:analysis* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D005069:Evaluation Studies as Topic; D008722:Methods; D009154:Mutation
D002273:Carcinogens; D004259:DNA-Directed DNA Polymerase