Specific direct fluorescent antibody identification of Mycobacterium leprae.
In vitro cultivation of M. leprae requires a rapid, specific identification procedure for monitoring the cultures. A method utilizing direct FITC-coupled lepromatous, specific serum globulin is described in detail with suggestions for improvement. After various purification and adsorption procedures, notably against human liver powder and M. tuberculosis, a fluorescent serum preparation is obtained which specifically reacts with M. leprae and not with other mycobacteria.
D000907:Antibodies, Bacterial; D000918:Antibody Specificity; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D009166:Mycobacterium leprae / Q000276:immunology* / Q000302:isolation & purification; D012712:Serum Globulins / Q000276:immunology
D000907:Antibodies, Bacterial; D003470:Culture Media; D012712:Serum Globulins