Organ distribution of immunocompetent cells in guinea pigs. II. Spleen, lymph node, bone marrow, or thymus cells in the restoration of the splenic plaque-forming cell response to sheep erythrocytes after irradiation.
The splenic plaque-forming cell response of inbred guinea pigs to sheep erythrocytes was abolished by whole-body X-irradiation with 600 rad. The minimum doses of various cell types from normal syngeneic donors to restore the response significantly were 100 X 10(6) spleen or lymph node cells or 500 X 10(6) bone marrow or thymus cells. The combination of 100 X 10(6) thymus and 100 X 10(6) bone marrow cells provided statistically significant restoration, but not much more than 100 X 10(6) bone marrow cells alone. The lack of thymus-marrow synergism suggests that in guinea pigs the antigen-reactive cells and antibody-forming cell precursors are not in the same way enriched in the thymus and bone marrow as in mice.
D000818:Animals; D000917:Antibody Formation / Q000528:radiation effects; D001853:Bone Marrow / Q000276:immunology*; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D006462:Hemolytic Plaque Technique; D008198:Lymph Nodes / Q000276:immunology* / Q000637:transplantation; D013154:Spleen / Q000276:immunology* / Q000637:transplantation; D013950:Thymus Gland / Q000276:immunology* / Q000637:transplantation; D014965:X-Rays