ATP concentration in Escherichia coli during oxygen toxicity.
Escherichia coli, strain E-26, grown in defined salts medium with glucose as the sole carbon and energy source, contained 1.50+/-0.16-10(6) molecules of ATP/cell. ATP was extracted with HC104 and assayed with a Dupont Luminescence Biometer using the luciferin-luciferase assay. Exposure during exponential growth at 37degreesC to 4.2 atm of oxygen resulted in complete growth cessation within 5 min, and to cyclic changes in cellular ATP concentration over a 2 h period. However, significant decrease in cellular ATP concentration occurred after growth inhibition in hyperbaric oxygen; hence, lack of ATP was not the cause of growth inhibition from oxygen toxicity.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000332:Aerobiosis; D000693:Anaerobiosis; D004121:Dimethyl Sulfoxide; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008163:Luminescent Measurements; D010100:Oxygen / Q000633:toxicity*; D013386:Succinates / Q000378:metabolism
D013386:Succinates; D000255:Adenosine Triphosphate; D005947:Glucose; D010100:Oxygen; D004121:Dimethyl Sulfoxide