A method of orientation of embedded bacterial colonies prepared for ultrathin sections.
The described method of embedding of bacterial colonies into media used for electron microscopy allows the precise orientation of the material for the preparation of ultrathin sections. The colonies are covered by a strip of agar joined to the agar-layer with the cultivated colonies by means of a heated razor-blade. This method is especially useful for ultrastructural studies of L-colonies.
D001431:Bacteriological Techniques; D008722:Methods