title: Hemolysis-in-gel and neutralization tests for determination of antibodies to mumps virus.
abstract: A hemolysis-in-gel test for the demonstration of antibodies to mumps virus is described. The results were compared with those of neutralization tests using a modified microtechnique. In the neutralization test viral replication was demonstrated by the hemadsorption of guinea pig erythrocytes, the visibility of which could be further enhanced by the use of o-tolidine. Good correlation was found between the results of the two techniques. The hemolysis-in-gel test was simple to perform, rapid, sensitive, and shown to be a useful test for the demonstration of mumps antibodies.
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D006462:Hemolytic Plaque Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D009107:Mumps / Q000175:diagnosis*; D009109:Mumps virus / Q000276:immunology*; D009500:Neutralization Tests; D014611:Vaccination
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M