title: Analysis of the kinetics of photoinduced crosslinkage of the protein and RNA components of the Escherichia coli 50 S ribosomal subunit.
abstract: The kinetics of photoinduced crosslinkage of the protein and ribosomal RNA components of the Escherichia coli 50 S ribosomal subunit were determined by two-dimensional gel electrophoresis. One group of ribosomal proteins was found to be reactive in the crosslinkage reaction at doses of 254 nm radiation of 2.9 X 10(3) ergs-mm-2. A second group of 50-S ribosomal proteins was found to be unreactive in the crosslinkage reaction at doses of 254 nm radiation less than 8.6 X 10(6) ergs-mm-2, but were reactive at higher doses.
mesh_terms: D004307:Dose-Response Relationship, Radiation; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000528:radiation effects*; D007700:Kinetics; D046911:Macromolecular Substances; D011485:Protein Binding; D012335:RNA, Ribosomal / Q000528:radiation effects*; D012269:Ribosomal Proteins / Q000528:radiation effects*; D012270:Ribosomes / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D046911:Macromolecular Substances; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins