[Comparative studies on the detection of Y-chromatin in native and formalin-fixed tissue or organ material (author's transl)].
Frequency of detectable Y-bodies in interphase nuclei after mepacrin staining of paraffin embedded formalin-fixed tissue was compared with those of cryostat sections of the native material and organ smears with and without formalin fixing. In formalin fixed smears from males 42--70% of investigated cells showed the Y-body. Sections of formalin-fixed and paraffin embedded male organs demonstrated in 12--24% of their cells the typical fluorescence for the Y-chromatin. High significantly less fluorescent Y-like bodies were found in smears and sections of all proofed organs from women prepared in the same manner. Mepacrin staining in combination with investigation of Barr-bodies permits a fast determination of sex of both organ smears and sections, too.
D001921:Brain / Q000166:cytology; D005453:Fluorescence; D006652:Histological Techniques; D006801:Humans; D007668:Kidney / Q000166:cytology; D008099:Liver / Q000166:cytology; D008297:Male; D012728:Sex Chromatin / Q000032:analysis*; D013154:Spleen / Q000166:cytology
Vergleichende Untersuchung zur Nachweisbarkeit des Y-Chromatins in nativen und formalinfixierten Organproben