title: A fluorescent antibody technique for identification of Mycoplasma hyopneumoniae colonies.
abstract: Fluorescent antibody staining of Mycoplasma hyopneumoniae colonies is difficult because the colonies wash from the agar. Airdrying of the colonies grown on solid medium-overlayed glass microscope slides fixed the colonies in place, so that fluorescent antibody stain could be readily accomplished. Apparent loss or alteration of antigenicity did not result from the air-drying process. The technique is useful for the identification of M hyopneumoniae isolates.
mesh_terms: D003429:Cross Reactions; D005455:Fluorescent Antibody Technique / Q000379:methods*; D009174:Mycoplasma / Q000254:growth & development / Q000276:immunology*