Two-dimensional electrophoresis of surface glycoproteins of normal BHK cells and ricin resistant mutants.
The surface glycoproteins of baby hamster kidney (BHK) cells were iodinated by lactoperoxidase and submitted to a two-dimensional electrophoresis procedure involving isoelectric focusing in the first dimension and SDS gel electrophoresis in the second dimension. After autoradiography a complex but reproducible pattern was obtained. The technique was then applied to the study of three ricin-resistant mutant clones with reduced rates of cell-cell and/or cell-substratum adhesion. Abnormal patterns were observed in all three mutant clones indicating different mechanisms of ricin resistance and identifying glycoproteins which may be involved in cellular interactions.
D002448:Cell Adhesion; D002450:Cell Communication; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D004351:Drug Resistance; D004586:Electrophoresis; D006023:Glycoproteins / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000302:isolation & purification*; D012276:Ricin / Q000494:pharmacology*
D006023:Glycoproteins; D008565:Membrane Proteins; D012276:Ricin