Immunofluorescence of spirochetes with serum from swine recovered from swine dysentery using an indirect fluorescent antibody test.
Using an indirect fluorescent antibody test, immunofluorescence of large spirochetes was observed with serum from swine that had recovered from swine dysentery. The spirochetes were obtained from scrapings of the colonic mucosa on the first day of diarrhea which was the time when the spirochete population was observed to be the highest. Of 29 exposed nonmedicated swine which developed and recovered from a diarrhea characteristic of swine dysentery 27 had antispirochete serum titers which ranged from 1:2 to 1:16. None of the 50 nonexposes swine developed a titer. Of 19 swine with a serum titer and reexposed with infective swine dysentery inoculum, 18 did not develop a diarrhea and were presumed to be immune. Considering these findings it is possible that this test could be used to detect antispirochete antibody in unknown swine serum.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D004403:Dysentery / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D005455:Fluorescent Antibody Technique; D013142:Spirochaeta / Q000302:isolation & purification*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology*
D000907:Antibodies, Bacterial