[Random immunoglobulin. I. Use of chaotropicions and fluorescence quenching for quantitating the non-specific adsorption in the interaction of human IgG with rhodamine B].
It is shown that, when excited in the visible range, fluorescence of the dye may be either quenched or enhanced, from its initial value Fo, by addition of increasing "normal" immunoglobulin (IgGH7 S), depending if the solution is "non-chaotropic " (NaF 0,1 M) or "chaotropic" KI 1 M). Let F be the fluorescence obtained at concentration P in protein, then the plot of the quantity P.F/(FO-F)against the quantity P.F/(FO-F) gives a straight line; the zero ordinate of this is the inverse of the non-specific interaction equilibrium constant. Quenching of fluorescence by increase of temperature, and by increase of concentration of salt (KI) are also quantitatively dealed upon in order to testify that this non-specific interaction is of the "adsorption" type. Concentration of dye is 10-6M; concentration of protein is varied from 0,05 % to 0,5 %.
D000327:Adsorption; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D011193:Potassium Iodide; D012235:Rhodamines; D014966:Xanthenes
D007074:Immunoglobulin G; D012235:Rhodamines; D014966:Xanthenes; D011193:Potassium Iodide
L'immunoglobuline au hasard. I. Utilisation des ions chaotropiques et de l'extinction de fluorescence pour l'evaluation quantitative de l'adsorption non spécifique a propos de l'interaction IgGH-rhodamine B