The use of diatomite microcolumns for the chromatographic separation of steroids prior to radioimmunoassay.
Since most anti-steroid antibodies are not usually specific enough to permit direct radioimmunoassay of these compounds, chromatographic purification is required to achieve reliability. We have used Celite microcolumns for the separation of a wide range of steroids prior to radioimmunoassay. Depending on the polarity of the steroids to be separated, various mixtures of solvents are used as stationary and mobile phases. This system is rapid, easy, economical, and reliable.
D002845:Chromatography / Q000379:methods*; D056890:Eukaryota; D011863:Radioimmunoassay / Q000592:standards; D013256:Steroids / Q000302:isolation & purification*
D013256:Steroids