Blockade of LH-RH induced ovulation in immature rats by LH-RH antiserum.
An in vivo method for the determination of biological potency of luteinizing hormone-releasing hormone (LH-RH) antiserum is described. The procedure involves antiserum blockade of LH-RH induced ovulation in immature rats primed with pregnant mare's serum. A modified method for the induction of LH-RH antibodies in rabbits is also detailed.
D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000037:antagonists & inhibitors* / Q000276:immunology; D006736:Horses / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D010060:Ovulation / Q000187:drug effects*; D011247:Pregnancy; D011817:Rabbits / Q000276:immunology; D051381:Rats
D000906:Antibodies; D007106:Immune Sera; D007987:Gonadotropin-Releasing Hormone