Evaluation of the genetic effects induced by vinyl chloride monomer (VCM) under mammalian metabolic activation: studies in vitro and in vivo.
As part of a programme of investigations on the biological effects of the industrial compound vinyl chloride monomer (VCM), the raw material for the production of polyvinyl chloride (PVC), analyses on the genetic effects by this compound have been done by experiments (in vitro) which have taken mammalian metabolism into account. Vinyl chloride in the presence of purified microsomes (sedimented at 105,000 g) obtained from mouse liver was converted into an active metabolite(s) which produced gene mutations in the yeast Schizosaccharomyces pombe (forward mutation) and gene conversions in two loci of a diploid Saccharomyces cerevisiae. Moreover, the compound was active in the host-mediated assay, when mice were treated with an oral dose of 700 mg/kg. The role is discussed of mutagenicity tests for the prediction of both genetic and carcinogenic risks of chemical compounds in industrial use.
D000818:Animals; D001203:Ascomycota / Q000378:metabolism*; D001711:Biotransformation; D004171:Diploidy; D004305:Dose-Response Relationship, Drug; D005821:Genetic Techniques; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009153:Mutagens; D009154:Mutation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012568:Schizosaccharomyces / Q000378:metabolism*; D014752:Vinyl Chloride / Q000378:metabolism* / Q000494:pharmacology; D014753:Vinyl Compounds / Q000378:metabolism*
D009153:Mutagens; D014753:Vinyl Compounds; D008741:Methyl Methanesulfonate; D014752:Vinyl Chloride