Transfer of paraphenylenediamine delayed-type hypersensitivity: a comparative investigation in the guinea pig, using arteriovenous cross-transfusion and parabiosis.
Arteriovenous cross-transfusion and parabiosis were used to study transfer of paraphenylenediamine (PPDA) hypersensitivity in guinea pigs. Nine out of 10 transfusion experiments were successful, resulting in definite positive test reactions in the recipient animals, and the remaining experiment was evaluated as possibly positive. Six out of 10 parabiosis experiments were successful, a further 2 were negative and the remaining 2 were evaluated as possibly positive. The mean test reactions in the recipients were stronger in the transfusion experiments than in the parabiosis experiments (0.001 less than P less than 0.01 for 0.5% PPDA). In control experiments, where the "donors" were nonsensitized guinea pigs negative test reactions were obtained. It was concluded that the transfusion technique was the most reliable of the 2 methods, when studying transfer of PPDA hypersensitivity.
D000818:Animals; D001803:Blood Transfusion / Q000379:methods; D004342:Drug Hypersensitivity / Q000276:immunology; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000276:immunology*; D007116:Immunization, Passive; D010227:Parabiosis; D010655:Phenylenediamines / Q000276:immunology
D010655:Phenylenediamines