N'-hydroxy-2-aminofluorene: the principal mutagen produced from N-hydroxy-2-acetylaminofluorene by a mammalian supernatant enzyme preparation.
A Salmonella typhimurium TA 1538 culture system wasused to monitor the production of mutagen from N-hydroxy-2-acetylaminofluorene by soluble liver enzymes. When benzene was used to extract the mutagen, no mutagenic activity remained in the liver enzyme preparation. The benzene extract contained approximately two-thirds of the total mutagenic activity produced by the liver enzyme preparation. Using thin layer and column chromatography to analyze the benzene extract, we deduced that N-hydroxy-2-aminofluorene accounted for the mutagenic activity which resulted from the incubation of N-hydroxy-2-acetylaminofluorene with soluble liver enzymes.
D015073:2-Acetylaminofluorene / Q000378:metabolism; D000818:Animals; D005449:Fluorenes / Q000378:metabolism*; D006881:Hydroxyacetylaminofluorene / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D006898:Hydroxylamines; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D009153:Mutagens; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects
D005449:Fluorenes; D006898:Hydroxylamines; D009153:Mutagens; D006881:Hydroxyacetylaminofluorene; C001368:N-hydroxy-2-aminofluorene; D015073:2-Acetylaminofluorene