title: Promotion of sporulation by caffeine pretreatment in Saccharomyces cerevisiae. I. Metabolism of nucleic acids and protein during sporulation.
abstract: Cells cultured in the presence of caffeine had high sporulation ability. The sporulation-promotive effect of caffeine was studied, special attention being paid upon changes in nucleic acid metabolism. When transferred to a sporulation medium, the breakdown of RNA, the synthesis of protein, RNA and DNA, commitment to sporulation and the appearance of mature asci took place in caffeine-treated cells significantly earlier than in control cells. Commitment to sporulation occurred before the completion of premeiotic DNA synthesis in both caffeine-treated and control cells.
mesh_terms: D002110:Caffeine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D005656:Fungal Proteins / Q000096:biosynthesis*; D009696:Nucleic Acids / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development; D013172:Spores, Fungal / Q000254:growth & development
chemical_list: D005656:Fungal Proteins; D009696:Nucleic Acids; D002110:Caffeine; D012313:RNA; D004247:DNA