Mechanization of a micro complement fixation test for the serodiagnosis of human toxoplasmosis.
A description is given of a mechanized micro complement-fixation test for the serodiagnosis of human toxoplasmosis. Besides a reduction in the quantity of reagents by using a microplate a considerable advantage of the test is the mechanization of actually all steps. Examination of large numbers of sera can be realized in a relatively short period of time. In order to test the sensitivity of the micro CF test comparative studies were carried out with the macro CF test and the immunofluorescence (IF) test on 549 human sera. The micro CF test proved to be more sensitive than the macro CF test. The results obtained with the micro CF test were in better agreement with the IF test than those obtained with the macro CF test.
D001322:Autoanalysis; D003168:Complement Fixation Tests / Q000295:instrumentation / Q000379:methods*; D005455:Fluorescent Antibody Technique; D014123:Toxoplasmosis / Q000175:diagnosis*