[Comparative study of group A streptococcal antigens. II. Comparative characteristics of several physico-chemical and immunochemical properties of partially purified M-protein and cytoplasmic protective antigen].
The authors carried out a comparative physico-chemical and immunochemical study of antigen obtained from the fraction of streptococcus cytoplasm by precipitation with ammonium sulfate, and partially purified M-protein. They proved to be heterogeneous by molecular-weight characteristics when tested by the methods of sedimentation analysis, disc-electrophoresis in polyacrylamide gel, and gel filtration on Sephadex G-200. Results of immunoelectrophoresis and disc-electrophoresis pointed to the presence in these two antigens of significant structural differences. A common component in M-protein and the cytoplasmic antigen was revealed by the immunodiffuse test. Spectrophotometric data pointed to the fact that M-protein represented protein, and cytoplasmic antigen was chiefly a nucleoproteid.
D000942:Antigens, Bacterial; D002473:Cell Wall / Q000276:immunology; D004589:Electrophoresis, Disc; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D011233:Precipitin Tests; D013053:Spectrophotometry; D013297:Streptococcus pyogenes / Q000276:immunology*
D000942:Antigens, Bacterial
Sravnitel'noe izuchenie antigenov streptokokka gruppy A. Soobshchenie II. Sravnitel'naia kharakteristika nekotorykh fiziko-khimicheskikh i immunokhimicheskikh svoĭstv chastichno ochishchennogo M-proteina i tsitoplazmaticheskogo zashchitnogo antigena