Simultaneous determination of endogenous norepinephrine and dopamine-beta-hydroxylase activity in biological materials by chemical ionization mass fragmentography.
A new clean-up procedure is described for the quantitation of biogenic amines and their metabolites by gas chromatography-chemical ionization mass fragmentography. The procedure is carried out in an acidic, non-aqueous medium. The metabolites as well as the amines are extracted with greater than 92% recovery. Using a CPG-10 column, the procedure is most suitable for the purification of these amines and their metabolites from the extract. It has been applied to the simultaneous determination of endogenous norepinephrine and dopamine-beta-hydroxylase (DBH) activity in rat adrenal by use of [2H2]dopamine as a substrate and [2H6]norepinephrine as an internal standard. The quantitation limit of the DBH activity is 0.1 pmole/mg of rat adrenal per hour.
D000311:Adrenal Glands / Q000032:analysis; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis; D004299:Dopamine beta-Hydroxylase / Q000097:blood / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D008722:Methods; D009638:Norepinephrine / Q000032:analysis* / Q000097:blood; D051381:Rats
D001679:Biogenic Amines; D004299:Dopamine beta-Hydroxylase; D009638:Norepinephrine