title: A study of the transmission and structure of double stranded RNAs associated with the killer phenomenon in Saccharomyces cerevisiae.
abstract: Killer strains contain two double stranded RNAs, L and M. The M dsRNA appears to be necessary for production of a toxin and for resistance to that toxin. Mutant strains have been found that are defective in their ability to kill and in their resistance to toxin. These sensitive, non-killer strains have altered dsRNA composition. One class has no M dsRNA. Another class of sensitive, non-killers called suppressives has no M dsRNA but instead has smaller dsRNAscalledS. Indiploidsresulting from a cross of a wild-type killer by a suppressive the transmission of the M dsRNA is suppressed by the S dsRNA. When a suppressive is crossed by a strain with no M dsRNA, the diploids and all four meiotic spores have the S dsRNA characteristic of the parental suppressive strain. Suppressive strains do not suppress each other. Intercrosses between two different suppressives yields diploids with both parental S dsRNAs. These two S dsRNAs are transmitted to all 4 meiotic progeny. Another class of mutants has been found which is defective for one of the traits but retains the other. One type, temperature-sensitive killers, has a normal dsRNA composition but is unable to kill at 30 degrees. The other type, immunity-minus, has a complex dsRNA pattern. The immunity-minus strain is extremely unstable during mitotic growth and segregates several different types of non-killers. Analysis of the dsRNAs from wild type and the mutants by electron microscopy shows that the L, M, and S dsRNAs are linear. All strains regardless of killer phenotype appear to have the same size L dsRNA.
mesh_terms: D002455:Cell Division; D003433:Crosses, Genetic; D008854:Microscopy, Electron; D008968:Molecular Conformation; D008970:Molecular Weight; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D012313:RNA / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014118:Toxins, Biological / Q000378:metabolism*
chemical_list: D014118:Toxins, Biological; D012313:RNA