Use of the Hungate anaerobic technique in the isolation of phloroglucinol-negative mutants of Coprococcus species.
The Hungate anaerobic technique was used with a standard procedure for bacterial mutagenesis employing N-methyl-N-nitro-N'-nitrosoguanidine to obtain mutants of an obligate anaerobe. Three mutant strains were derived from a Coprococcus sp., strain Pe15, a rumen anaerobe capable of growing on phloroglucinol. The mutants did not grow on phloroglucinol but did degrade the compound in anaerobic washed-cell suspensions, producing the same end products in approximately the same proportions as the wild type. It was concluded that the mutants were blocked in a unique step or steps necessary for carbon skeleton or energy synthesis from phloroglucinol and not in formation of an enzyme involved in the pathway of phloroglucinol degradation.
D000693:Anaerobiosis; D000818:Animals; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D001431:Bacteriological Techniques; D002245:Carbon Dioxide / Q000096:biosynthesis; D005232:Fatty Acids, Volatile / Q000096:biosynthesis; D008769:Methylnitronitrosoguanidine; D009153:Mutagens; D009154:Mutation; D010696:Phloroglucinol / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*
D005232:Fatty Acids, Volatile; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D002245:Carbon Dioxide; D010696:Phloroglucinol