[A study of the reaction between antibodies and DNA by the method of immune complex formation on the membranes of nitrocellulose filters].
The conditions were developed to measure antibodies to denatured and native DNA. It was shown that membrane filters treated with alkali adsorbed denatured DNA complexed with antibodies. Utilizing this method it was shown that antibodies to denatured DNA react with pyrimidines (probably thymine). This assay provides proof of the wide existence antibodies to native DNA in the sera of patients with systemic lupus erythematosus. DNA-binding of sera was significantly depended on reaction condition.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D002417:Cattle; D002482:Cellulose; D004247:DNA / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis; D005374:Filtration; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009574:Nitro Compounds
D000906:Antibodies; D004269:DNA, Bacterial; D009574:Nitro Compounds; D002482:Cellulose; D004247:DNA
Izuchenie reaktsii mezhdu antitelami i DNK metodom fiksatsii immunykh kompleksov na membrannykh nitrotselliuloznykh fil'trakh