Identification of basophilic granulocytes in human leukocyte suspensions subjected to immunofluorescence procedures with anti-IgE.
A method is described by which human leukocyte preparations obtained by Ficoll-Isopaque centrifugation are stained and fixed in such a way that the basophilic granulocytes in these preparations can be identified when the latter are subjected to immunofluorescence procedures with anti-IgE. Capping, blocking and absorption experiments confirmed the specificity of the test.
D000888:Antibodies, Anti-Idiotypic; D001491:Basophils / Q000276:immunology*; D001667:Binding, Competitive; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006801:Humans; D007073:Immunoglobulin E; D007962:Leukocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D000888:Antibodies, Anti-Idiotypic; D011947:Receptors, Antigen, B-Cell; D007073:Immunoglobulin E