title: Translation of T7 RNA in vitro without cleavage by RNase III.
abstract: T7 early mRNA's are generated from a high-molecular-weight precursor RNA by site-specific RNase III cleavage. When T7 DNA is transcribed in vitro by Escherichia coli RNA polymerase, the transcript is a large, single-piece RNA equivalent to the in vivo precursor RNA. The T7 RNA synthesized in vitro can be translated as a polycistronic messenger without cleavage by RNase III. All T7 early proteins are synthesized in an RNase III-free, protein-synthesizing system directed by the uncleaved T7 RNA.
mesh_terms: D002474:Cell-Free System; D003090:Coliphages / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D008970:Molecular Weight; D009694:Nucleic Acid Precursors / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D009694:Nucleic Acid Precursors; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases