A simple method for detection and analysis of carcinogenic nitrofuran compounds and their metabolites by combining chromatography and spot mutation tests.
A simple, sensitive, and convenient method combining thin-layer chromatography and a spot test for mutagenicity of Salmonella typhimurium TA100 was utilized for the analysis of urine of rats fed N-[4-(5-nitro-2-furyl)-2-thiazolyl]-formamide (FANFT), a potent experimental urinary bladder carcinogen. Three metabolites of FANFT were detected in urine, and one of these, accounting for 33% of the urinary metabolites of FANFT, was identified as 2-amino-4-(5-nitro-2-furyl)thiazole (ANFT). ANFT was the only urinary metabolite that clearly demonstrated mutagenic activity, suggesting that ANFT may be a proximate vesical carcinogen of FANFT.
D000818:Animals; D002855:Chromatography, Thin Layer; D005260:Female; D008722:Methods; D009153:Mutagens / Q000302:isolation & purification*; D009581:Nitrofurans / Q000652:urine*; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000652:urine*
D009153:Mutagens; D009581:Nitrofurans