[Immunofluorescence and immunoelectron microscopic study of changes occurring in secondary reaction of popliteal lymph nodes in young and old rats].
A comparative study of the secondary reaction of popliteal lymph nodes in young and adult rats was carried out. In one group of experiments secondary reaction was induced by bovine serum albumin (BSA), in the other one by peroxidase from horseradish. Antigen was administered with Freund adjuvant. It was established that the index of popliteal lymph nodes in a secondary reaction induced by BSA does not change with the increasing age. The incidence of antibody-producing cells in young and adult rats is the same. In the cytoplasma and mitochondria of the antibody-producing cells of the adult animals degenerative changes were observed. In the secondary reaction brought about by peroxidase intracytoplasmatic localization of the antibody in the young and adult animals did not reveal any differences.
D000367:Age Factors; D000921:Antibody-Producing Cells; D004721:Endoplasmic Reticulum / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006735:Horseradish Peroxidase; D008198:Lymph Nodes / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008928:Mitochondria / Q000276:immunology; D012710:Serum Albumin, Bovine
D012710:Serum Albumin, Bovine; D006735:Horseradish Peroxidase
Fiatal és öreg patkányok poplitealis nyirokcsomóinak secundaer secundaer reakciójában fellépö változások immunfluorescens- és immunelektronmikroszkópos vizsgálata