Membrane attachment of folded chromosome of Escherichia coli.
By fractionation of the envelope part of membrane-associated folded chromosomes it is shown that only the outer membrane is bound to the DNA. Experiments with the M-band technique suggest the presence of attachment points for the membrane also in membrane-released folded chromosomes.
D001433:Bacteriolysis; D001665:Binding Sites; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000648:ultrastructure*; D008274:Magnesium; D012521:Sarcosine / Q000031:analogs & derivatives; D013696:Temperature
D004269:DNA, Bacterial; D008274:Magnesium; D012521:Sarcosine