Penicillin-induced lysis of Escherichia coli in medium that does not support sustained growth.
Exponentially growing cells of an Escherichia coli auxotroph that are washed and resuspended in buffer containing a carbon source undergo sufficient growth to render them susceptible to penicillin-induced lysis, even in the absence of the required metabolite. This lysis is probably responsible for the reduction in intracellular enzyme activity previously attributed to a direct effect of penicillin upon the enzyme.
D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D010406:Penicillins / Q000494:pharmacology*; D011392:Proline / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D010406:Penicillins; D011392:Proline; D005947:Glucose