Glycerinated glycol methacrylate mounting medium in highlighting non-coated or glycerin-substituted microsamples under the scanning electron microscope.
Glycol methacrylate hardened with 10-15% glycerin showed no charging and gave dark contrast in the scanning electron microscope with an acceleration voltage of 25kV and a specimen current of 1x10(-10) A. Using human blood cells, it was shown that this conductively treated resin is a useful mounting substrate to highlight the non-coated microsamples or to obtain their highly contrasted images under the scanning electron microscope. It was also demonstrated that the glycerin-substituted blood cells can be scanned with little shrinkage.
D000179:Acrylates; D003584:Cytological Techniques; D004912:Erythrocytes / Q000648:ultrastructure; D005990:Glycerol; D006018:Glycols; D006801:Humans; D008689:Methacrylates; D008855:Microscopy, Electron, Scanning
D000179:Acrylates; D006018:Glycols; D008689:Methacrylates; D005990:Glycerol