title: Effect of rat stalk median eminence extracts on rat GH secretion in vivo.
abstract: Estradiol (0.2 mug), injected subcutaneously for 10 days to adult male rats, increased plasma growth hormone (GH) levels as compared with oil-treated controls. In estradiol-pretreated (10 days), urethane-anesthetized rats, the first as well as the second of two successive intracarotid injections, at 1-hour intervals, of one rat stalk median eminence equivalent evoked a significant rise in plasma radioimmunoassayable GH. Under the same conditions, cerebral cortex extracts (1 equivalent) induced a slight elevation whereas vasopressin (30 mU) or serotonin (200 ng) were ineffective. These results indicate that estrogen-primed, urethane-anesthetized rats can be used to demonstrate GH-releasing activity in rat SME extracts.
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000502:physiology; D004958:Estradiol / Q000494:pharmacology; D013006:Growth Hormone / Q000097:blood*; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology*; D008297:Male; D008473:Median Eminence / Q000502:physiology*; D051381:Rats; D012701:Serotonin / Q000494:pharmacology; D014020:Tissue Extracts; D014667:Vasopressins / Q000494:pharmacology
chemical_list: D014020:Tissue Extracts; D014667:Vasopressins; D012701:Serotonin; D004958:Estradiol; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone