Estimation of Mycoplasma pneumoniae inoculum size by rate of tetrazolium reduction.
The rate of appearance of red-colored formazan indicating reduction of 2,3,5,-triphenyltetrazolium chloride by Mycoplasma pneumoniae was dependent on the inoculum size. The test permits estimation of M. pneumoniae cells in preparations that cannot be counted by dilution methods.
D001431:Bacteriological Techniques; D002452:Cell Count; D005562:Formazans / Q000096:biosynthesis; D007202:Indicators and Reagents; D009174:Mycoplasma / Q000302:isolation & purification* / Q000378:metabolism; D010084:Oxidation-Reduction; D013778:Tetrazolium Salts / Q000378:metabolism
D005562:Formazans; D007202:Indicators and Reagents; D013778:Tetrazolium Salts