Nonsuppressible insulin-like activity in rat organs as detected by fluorescent antibody and radioimmunoassay techniques.
NSILA-S was localized by immunofluorescent antibody staining in the exocrine part of the pancreas and the submaxillary salivary gland of the rat, where part of the cells give positive reactions. To a lesser degree it was demonstrated in the kidney, where some--probably the actually functioning--nephrons give positive staining reactions within their tubular cells. Radioimmunoassayable NSILA-S was found in extracts of the pancreas (120-550 ng/g), of the submaxillary salivary gland (220-330 ng/g) and of the kidney (45 ng/g), whereas liver (and some other organs) contain practically no NSILA-S.
D000311:Adrenal Glands / Q000032:analysis; D000818:Animals; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000032:analysis; D007413:Intestinal Mucosa / Q000032:analysis; D007668:Kidney / Q000032:analysis; D008297:Male; D009928:Organ Specificity; D010179:Pancreas / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D013363:Submandibular Gland / Q000032:analysis; D013737:Testis / Q000032:analysis; D013002:Somatomedins / Q000032:analysis*
D013002:Somatomedins