title: The use of agarose gels for the assay of the deoxyribonuclease activity associated with the soluble penton of adenoviruses.
abstract: Evidence is presented that the excess pool of penton complexes purified from adenovirus type 1-infected HEp-2 cells also carry an endonucleolytic activity. The effect was measured with both E. coli and adenovirus type 1 double-stranded DNA substrates. The use of agarose gels for assaying the endonuclease activity allowed the approximative calculation of the number of penton molecules which might produce one DNA double-strand break under the experimental conditions selected.
mesh_terms: D000260:Adenoviruses, Human / Q000201:enzymology* / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D006868:Hydrolysis; D012995:Solubility; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D014764:Viral Proteins; D003851:Deoxyribonucleases; D004720:Endonucleases