Distribution of dengue-2 antigens by electron immunocytochemistry.
The distribution of dengue-2 antigens was studied in infected monkey kidney cells (LLC MK2) using an indirect, horseradish peroxidase-conjugated immunoglobulin technique. This procedure allowed both light and electron microscopic examination of serial-step sections of individual cells cut in a plane perpendicular to the monolayer. Both virion and nonvirion antigens were identified on the plasma membrane with this technique. A diffuse cytoplasmic reaction product was also present. The intensity and distribution of the cytoplasmic reaction product was related to disruption of the plasma membrane.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002462:Cell Membrane / Q000648:ultrastructure; D003716:Dengue Virus / Q000276:immunology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000276:immunology*; D051379:Mice; D008854:Microscopy, Electron
D000956:Antigens, Viral