title: Plasma protein immunocytochemistry and polysaccharide cytochemistry at the surface of alveolar and endothelial cells in the rat lung.
abstract: Ultrastructural immunocytochemistry of plasma proteins and cytochemistry of polysaccharides with ruthenium red and concanavalin A were combined with different types of fixation with or without prior vascular or airway washing, to study the surface of capillary endothelial and alveolar epithelial cells of the blood-air barrier in the rat lung. The endothelial and epithelial cell surface layers were found to have two components: a moveable part belonging to the cellular microenvironement made of plasma proteins and a deeper glucidic or anionic fixed part bound to the plasma membranes (cell coat).
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D004727:Endothelium / Q000032:analysis / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D008168:Lung / Q000032:analysis* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000032:analysis*; D011650:Pulmonary Alveoli / Q000032:analysis*; D051381:Rats
chemical_list: D001798:Blood Proteins; D011134:Polysaccharides