Establishment of the origin of a porcine cell line by chromosome banding techniques and scanning.
Use of cultured cells in diagnostic virology implies the necessity to establish the genetic origin of the cells. Chromosome analysis, performed on a cell line of pig embryo kidney cells (PEK), made the porcine origin of the cells plausible. Definite proof, however, was furnished by chromosome banding and scanning. A comparison was made between PEK-cells and normal pig chromosomes. One of the chromosomes No. 1 had an interstitial deletion, which provided an useful marker for identification purposes.
D000818:Animals; D002460:Cell Line; D002872:Chromosome Deletion; D002875:Chromosomes; D005821:Genetic Techniques; D007621:Karyotyping; D007668:Kidney / Q000166:cytology; D013552:Swine