Membrane lipoteichoic acid of Streptococcus pyogenes and its stabilized L-form and the effect of two antibiotics upon its cellular content.
Membrane lipoteichoic acid continues to be synthesized by an osmotically fragile, stabilized L-form of Streptococcus pyogenes. Chromatographic and electrophoretic comparisons indicate that the lipid componenent of lipoteichoic acid in this L-form and its parental streptococcus is glycerophosphoryldiglucosyl diglyceride and not phosphatidylkojibiosyl diglyceride. Based upon dry weight determinations, the yield of lipoteichoic acid from the L-form is 0.19%, as compared with 0.97% from the streptococcus. When grown with bacitracin the L-form contains the same amount of teichoic acid as when grown without this antibiotic; however, its lipoteichoic acid content is reduced by 85%. Similarly, the L-form grown with novobiocin for 10 h contains only 17% of the teichoic acid found in control cells.
D001414:Bacitracin / Q000494:pharmacology*; D006017:Glycolipids / Q000096:biosynthesis*; D007740:L Forms / Q000378:metabolism*; D009675:Novobiocin / Q000494:pharmacology*; D013297:Streptococcus pyogenes / Q000378:metabolism*; D013682:Teichoic Acids / Q000096:biosynthesis*
D006017:Glycolipids; D013682:Teichoic Acids; D001414:Bacitracin; D009675:Novobiocin