Direct hydroxylation in the biosynthesis of hydroxy fatty acid in lipid a of Pseudomonas ovalis.
It was found that Pseudomonas ovalis IAM 1177 had an abundance of hydroxy fatty acids such as 3-hydroxy-decanoic acid, 3-hydroxy-dodecanoic acid and 2-hydroxy-dodecanoic acid in the lipophilic part of the lipopolysaccharide fraction, which comprise 80% of total fatty acids. By using 18O2, it was shown that one oxygen atom from molecular oxygen was incorporated into 2-hydroxy-dodecanoic acid, but not into 3-hydroxy-decanoic acid. The incorporated oxygen atom was specifically located at the hydroxyl group of 2-hydroxy-dodecanoic acid. The biosynthetic pathways of these hydroxy fatty acids are discussed.
D005227:Fatty Acids / Q000096:biosynthesis; D006880:Hydroxy Acids / Q000096:biosynthesis*; D006900:Hydroxylation; D050356:Lipid Metabolism; D008954:Models, Biological; D010101:Oxygen Consumption; D011549:Pseudomonas / Q000378:metabolism*
D005227:Fatty Acids; D006880:Hydroxy Acids