title: Organization and energy-dependent growth of microtubules in cells.
abstract: The organization and growth of microtubules in cultured mouse macrophages and fibroblasts were examined by indirect immunofluorescence microscopy with antibodies to microtubule protein. In macrophages, microtubules converged at a samll region at the cytocenter. During depolymerization, and repolymerization, this region acted as a microtubule organizing center. Microtubule growth was energy-dependent, but unaffected by dibutyryl-adenosine 3':5'-cyclic monophosphate, cholera toxin, or dibutyryl-guanosine 3':5'-cyclic monophosphate. Fibroblasts, which did not show such a simple microtubule organization as macrophages, contained mainly one or two, but occasionally as many as four, organizing centers during repolymerization. These microtubule organizing centers often appeared as fluorescent rings with a dark center.
mesh_terms: D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D003080:Cold Temperature; D003703:Demecolcine / Q000494:pharmacology; D004734:Energy Metabolism; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D008264:Macrophages / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D009712:Nucleotides, Cyclic / Q000494:pharmacology
chemical_list: D009712:Nucleotides, Cyclic; D003078:Colchicine; D003703:Demecolcine