Immunofluorescent localisation of cathepsin D in trophoblastic cells in tissue culture.
Cathepsin D was localised by immunofluorescence within four different types of cells derived from human trophoblast in monolayer culture. Cells were stained with freshly reduced Fab' fragments of sheep anti-(human cathepsin D) antiserum followed by FITC conjugate of pig anti-(sheep Fab') Fab' fragments. The green fluorescence seen in cytoplasmic droplets were characteristic of the appearance of lysosomes in other cells.
D002403:Cathepsins / Q000032:analysis*; D002478:Cells, Cultured; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D011247:Pregnancy; D014327:Trophoblasts / Q000201:enzymology*
D002403:Cathepsins