Rhodanese from Thiobacillus A2: determination of activity by proton nuclear magnetic resonance spectroscopy.
Rhodanese from Thiobacillus A2 was shown by proton nuclear magnetic resonance (NMR) spectroscopy to use dihydrolipoate or dihydrolipoamide as acceptor of the sulphane moiety of thiosulphate with the formation of alpha-lipoate or lipoamide respectively. Correlation is shown between assays of the enzyme activity by NMR spectroscopy and by ultraviolet spectrophotometry.
D000577:Amides / Q000096:biosynthesis; D002474:Cell-Free System; D009682:Magnetic Resonance Spectroscopy; D013466:Sulfurtransferases / Q000378:metabolism*; D013855:Thiobacillus / Q000201:enzymology*; D008063:Thioctic Acid / Q000096:biosynthesis; D013884:Thiosulfate Sulfurtransferase / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism
D000577:Amides; D013885:Thiosulfates; D008063:Thioctic Acid; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase