Bromodeoxyuridine 5'-monophosphate incorporation into yeast nuclear and mitochondrial deoxyribonucleic acid.
Standard laboratory yeast strains can be enriched for thymidine 5'-monophosphate (TMP) uptake derivatives that generate only a low percentage of respiratory-deficient colonies (petites) under inhibition of TMP biosynthesis. Such mutants incorporated bromodeoxyuridine 5'-monophosphate (BrdUMP) into both nuclear and mitochondrial deoxyribonucleic acid (mtDNA); however, they showed a selectivity for TMP over BrdUMP incorporation. The preferential incorporation of [3H]TMP or BrdUMP into mtDNA was strain dependent. The density increments after growth in the presence of BrdUMP reached 50 mg/ml for nuclear DNA and 22 mg/ml for mtDNA in CsCl gradients. Density shifts corresponding to 4% bromouracil substitution were easily detected. Preliminary density transfer experiments confirm that mtDNA does not replicate in synchrony with nuclear DNA.
D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D008928:Mitochondria / Q000378:metabolism; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000378:metabolism; D014500:Uracil Nucleotides / Q000378:metabolism*
D013942:Thymine Nucleotides; D014500:Uracil Nucleotides; D004247:DNA; D001973:Bromodeoxyuridine