In vitro processing of E. coli tRNA precursors.
Using E. coli tRNA precursors isolated from an RNAase P mutant strain, we have studied the steps required for the formation of tRNAs having a mature primary sequence in vitro. Our results suggest that at least three different enzymatic activities can participate in the processing of tRNA precursors.
D004926:Escherichia coli / Q000378:metabolism*; D009694:Nucleic Acid Precursors / Q000378:metabolism*; D012329:RNA, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000032:analysis / Q000096:biosynthesis*; D012260:Ribonucleases / Q000378:metabolism
D009694:Nucleic Acid Precursors; D012329:RNA, Bacterial; D012343:RNA, Transfer; D012260:Ribonucleases