Production of specific antisera to human B lymphocytes.
Antisera have been prepared, in mice and rabbits, to membrane and sub-membrane fractions of human B lymphocyte derived lymphoid lines. Antisera to a protein subfraction were, after only minimal absorption, specific for human peripheral B lymphocytes, monocytes and B cell derived lymphoid lines. The antigen(s) recognised by these antisera were not the same as the previously described B-cell markers; immunoglobulin, Fc receptor, complement receptor and Ia antigens. The antigen(s) could not be removed from cells by lysostrip with anti- beta2 microglobulin.
D000042:Absorption; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens; D006801:Humans; D007104:Immune Adherence Reaction; D007106:Immune Sera / Q000302:isolation & purification; D051379:Mice; D009000:Monocytes / Q000276:immunology; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology
D006680:HLA Antigens; D007106:Immune Sera; D003165:Complement System Proteins