[Isolation and function of platelets. I. Platelet rich plasma. Comparison between 2 methods: gel filtration and albumin density gradient centrifugation. II. A new method using total blood: metrizamide gradient centrifugation].
Human platelets separated from platelet rich plasma (PRP) by two different methods: gel filtraton and centrifugation on albumin gradient have been compared for yield, cellular and protein contamination, ultra-structure, platelet populations, shape change, aggregability and functional preservation. A new method for the separation of platelets from total blood on metrizamide gradients has been established. This technique is rapid and easy; it avoids the initial centrifugation of the PRP where about 30% of the platelets are lost and needs of blood 5 to 10 ml of blood.
D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D006801:Humans; D008793:Metrizamide; D008858:Microscopy, Phase-Contrast; D010974:Platelet Aggregation; D012310:Ristocetin / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism
D012310:Ristocetin; D012701:Serotonin; D008793:Metrizamide
Isolement et fonctions des plaquettes I. A partir de plasma riche en plaquettes. Comparaison de deux méthodes: filtration sur gel et centrifugation sur gradient d'albumine. II. Nouvelle méthode à partir de sang total: centrifugation sur gradients de métrizamide