Mutagenicity and toxicity of amitrole. III. Microbial tests.
Amitrole (3-amino-1,2,4-triazole) inhibits bacterial growth both in Escherichia coli and Salmonella typhimurium at a concentration of 0.5% in minimal medium. Repression of growth already occurs at a concentration of 0.1% of amitrole in this medium. In complete medium the bacteria tolerate concentrations of amitrole as high as 1.7-2.4% before growth ceases. Mutagenicity was tested by differential growth comparisons on E. coli strains W 3110 thy pol A1, defective in DNA polymerase I, and its revertant pol A+. Known mutagens (MMS, NTG, mitomycin C) were used as positive controls. Analogous negative results were also obtained in a revertant test when several trp mutant strains of Salmonella were used.
D000640:Amitrole / Q000494:pharmacology*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004260:DNA Repair / Q000187:drug effects; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D009153:Mutagens; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D014230:Triazoles / Q000494:pharmacology*
D004269:DNA, Bacterial; D009153:Mutagens; D014230:Triazoles; D004254:DNA Nucleotidyltransferases; D000640:Amitrole