A simplified procedure for preparing myogenic cells for culture.
A procedure involving mechanical agitation referred to as vortexing is compared to a trypsin procedure for obtaining myogenic cells for culture. The vortex procedure appears to be at least as useful as the trypsin procedure and has several advantages including speed, the elimination of chemical disruptive agent, elimination of collagen coating of culture dishes and earlier onset of fusion.
D000818:Animals; D002459:Cell Fusion; D002467:Cell Nucleus; D002469:Cell Separation; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D003584:Cytological Techniques; D009119:Muscle Contraction; D009132:Muscles; D014357:Trypsin
D003470:Culture Media; D014357:Trypsin