Pressure-jump relaxation studies of the association--dissociation reaction of E. coli ribosomes.
The association--dissociation kinetics of ribosomal particles from E. coli have been studied using a pressure-jump apparatus with optical detection. Experiments on isolated subunits yield two relaxation times of about 10 and 700 ms, respectively. With mixtures of 30 S and 50 S particles an additional relaxation time of about 100 ms is observed, which is assigned to the equilibrium 30 S + 50 S in equilibrium 70 S. The two other times are attributed to reversible equilibria between subunit monomers and subunit homo-associates.
D001665:Binding Sites; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008433:Mathematics; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism*; D011312:Pressure; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D008274:Magnesium