Analysis of enzyme induction in bacteria.
The theoretical relations between the induced initiation and accumulation of lac mRNA and its translation are derived, taking the kinetics of repressor-operator dissociation and enzyme maturation into account. These relations are used to evaluate observed data on lac induction and to estimate a number of parameters that characterize the transcription and translation of the beta-galactosidase gene in the bacterium Escherichia coli B/r growing at three different rates (0.7-2.1 doublings/h).
D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology*; D005696:Galactosidases / Q000096:biosynthesis; D007700:Kinetics; D008954:Models, Biological; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D005696:Galactosidases