title: Further purification of group A streptococcal pyrogenic exotoxin and characterization of the purified toxin.
abstract: Streptococcal pyrogenic exotoxin (SPE) isolated from culture filtrates of strain NY-5 (type 10), and separated from other extracellular by differential solubility in ethanol and acetate-buffered saline, has previously been shown to exhibit a wide range of biological activities including erythrogenic activity, pyrogenicity, enhancement of susceptibility to endotoxin shock, blockage of the reticuloendothelial system immmunosuppression, and lymphocyte mitogenicity. Toxin prepared in this way was found to consist of hyaluronic acid and several proteins which could be distinguished by thin-layer polyacrylamide isoelectric focusing (IEF), SPE has been further purified by ion exchange chromatography on QAE-Sephadex columns. One of the fractions isolated from QAE-Sephadex, and shown to be a homogenous protein by thin-layer IEF and Ouchterlony with hyperimmune serum, was highly active erythrogenically, pyrogenically, and in enhancing susceptibility to endotoxin. This fraction was identified as exotoxin A. A second, less active fraction identified as SPE B showed similar activities, but differed from the other fraction antigenically and in net charge and molecular weight. These findings indicate that a single highly purified protein can mediate at least three of the biological activities attributed to SPE and NY-5 produces pyrogenic exotoxins A and B in vitro as well as in vivo.
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D006820:Hyaluronic Acid / Q000032:analysis; D011749:Pyrogens / Q000032:analysis*; D012882:Skin Tests; D013297:Streptococcus pyogenes / Q000032:analysis*; D013329:Structure-Activity Relationship; D014118:Toxins, Biological / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D011749:Pyrogens; D014118:Toxins, Biological; D006820:Hyaluronic Acid