A haemolytic spot test for the estimation of alloantibody against antigens of the major histocompatibility complex.
A haemolytic technique for the assay of alloantibody has been developed. Titration of alloantiserum was accomplished by placing microdroplets of serum dilutions on agarose layers containing the appropriate erythrocytes. Complement-mediated lysis was procured after the addition of rabbit serum. The assay appeared specific for products of the major histocompatibility gene complex in the rat and in the mouse.
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D003165:Complement System Proteins; D004912:Erythrocytes / Q000201:enzymology; D006461:Hemolysis; D006649:Histocompatibility Antigens / Q000032:analysis*; D007106:Immune Sera / Q000494:pharmacology; D007111:Immunity, Cellular; D007158:Immunologic Techniques; D007518:Isoantibodies / Q000032:analysis*; D051379:Mice; D008811:Mice, Inbred DBA; D011817:Rabbits; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D016038:Skin Transplantation; D014184:Transplantation, Homologous
D006649:Histocompatibility Antigens; D007106:Immune Sera; D007518:Isoantibodies; D003165:Complement System Proteins