title: Migration inhibition factor-like activity in inflammatory synovial fluids might be due to proteases.
abstract: Using an assay of macrophage migration, where the cells emigrate from an agarose droplet, it was found that the neutral proteases trypsin, chymotrypsin, Pronase and elastase have MIF-like activity. Appropriate enzyme inhibitors counteract this effect. To twelve synovial fluids from patients with inflammatory arthritis, which have MIF-like activity (migration index between 0-3 and 0-7) protease indhibitors (Trasylol, ovomucoid and soybean inhibitor) were added. Ten of the fluids lost some of their MIF-like activity with at least one inhibitor. Phenylmethylsulphonylfluoride counteracted totally the MIF-like activity of the two fluids tested. It is concluded that MIF-like activity of inflammatory synovial fluids is due, at least partially, to proteases.
mesh_terms: D000818:Animals; D000941:Antigens; D002918:Chymotrypsin / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D008263:Macrophage Migration-Inhibitory Factors / Q000037:antagonists & inhibitors; D008264:Macrophages / Q000276:immunology; D051379:Mice; D010196:Pancreatic Elastase / Q000276:immunology; D010447:Peptide Hydrolases / Q000276:immunology*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011402:Pronase / Q000276:immunology; D013582:Synovial Fluid / Q000276:immunology*; D014357:Trypsin / Q000276:immunology
chemical_list: D000941:Antigens; D008263:Macrophage Migration-Inhibitory Factors; D010664:Phenylmethylsulfonyl Fluoride; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin; D011402:Pronase