[Indication of halprowia (chlamydia) antigens by the direct immunoperoxidase method].
The authors modified and approbated the direct immunoperoxidase method for the indication in the infected L cells of the halprowia (chlamydia) antigens--etiological agents of trachoma, ocular and urogenital forms of paratrachoma, arthritis, meningopneumonia and enzootic abortion of sheep. Different types of localization of the group-specific halprowia antigen were revealed in the cytoplasm of the affected cells by light microscopy; electron microscopy demonstrated localization of this antigen in the membrane of the cell wall of the elementary and initial bodies. Specificity of the test-system used, no lesser sensitivity than the sensitivity of direct immunofluorescent method of indication of the same microbies, with a possibility of detection of stably stained antigens by widely accessible light microscopy underly practical usefulness of direct immunoperoxidase method of the diagnosis of halprowiosis (chlamydiosis) of man and animals.
D000942:Antigens, Bacterial; D002689:Chlamydia / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007739:L Cells / Q000276:immunology / Q000382:microbiology*
D000942:Antigens, Bacterial
Indikatsiia antigenov gal'proviĭ (khlamidiĭ) priamym immunoperoksidaznym metodom