A new method of radioiodine labeling of polyanionic macromolecules for radioimmunoassay.
A method is described for radiolabeling polyanionic macromolecules by the formation of an electrostatic complex with a radioiodinated tyramine conjugated DEAE-dextran. Data is presented on the use of this technique for the detection and quantitation of immunospecific binding of polynitrophenylated proteins, deoxyribonucleic acids, and cell surface antigens.
D000838:Anions; D000906:Antibodies / Q000032:analysis; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis; D002462:Cell Membrane / Q000276:immunology; D003637:DEAE-Dextran; D004247:DNA / Q000276:immunology; D007158:Immunologic Techniques; D007457:Iodine Radioisotopes; D007518:Isoantibodies / Q000032:analysis; D007526:Isoelectric Point; D009578:Nitrobenzenes / Q000276:immunology; D011863:Radioimmunoassay; D014439:Tyramine
D000838:Anions; D000906:Antibodies; D000941:Antigens; D007457:Iodine Radioisotopes; D007518:Isoantibodies; D009578:Nitrobenzenes; D004247:DNA; D003637:DEAE-Dextran; D014439:Tyramine