[Radiolabeling (125I) with high specific activity of the 1-34 N-terminal derivative of bovine parathormone without loss of biological activity].
Radiolabelling of 1-34 N terminal synthetic biological active fragment of bovine parathormone (1-34 bPTH) with high specific activity and without loss of biological activity was studied. Chloramine T and lactoperoxydase methods were used. To assess biological activity of 125I 1-34 bPTH labelled fragment, we use their ability to activate renal cortical adenylate cyclase. Our results show that enzymatic labelling method preserved the biological activity. With the chloramine T method it is possible to keep the biological activity by using DMSO.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002700:Chloramines; D004121:Dimethyl Sulfoxide; D006728:Hormones; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D007672:Kidney Cortex / Q000201:enzymology; D007784:Lactoperoxidase; D010281:Parathyroid Hormone / Q000502:physiology*; D010446:Peptide Fragments; D011485:Protein Binding; D051381:Rats
D002700:Chloramines; D006728:Hormones; D007457:Iodine Radioisotopes; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D007784:Lactoperoxidase; D000262:Adenylyl Cyclases; D004121:Dimethyl Sulfoxide
Radiomarquage (125I) a haute activité spécifique du dérivé 1-34 N-terminal de la parathormone bovine sans perte de l'activité biologique