Radiometric studies of Mycobacterium lepraemurium.
The radiometric method has been applied for studying the metabolism of M. lepraemurium and the conditions which might force or inhibit its metabolic activity in vitro. These organisms assimilate and oxidize (U-14C) glycerol, and (U-14C) acetate, but are unable to oxidize (U-14C) glucose, (U-14C) pyruvate, (U-14C) glycine and 14C-formate. When incubated at 30 degrees C M. lepraemurium oxidizes (U-14C) acetate to 14CO2 faster than 37 degrees C. The smae effect was observed with increasing concentrations of polysorbate 80 (Tween 80), or the 14C-substrate. No change in metabolic rate was observed when the organisms were kept at -20 degrees C for 12 days. Although tried several times, it was not possible to demonstrate any "inhibitors" of bacterial metabolism in the reaction system. The radiometric method seems to be an important tool for studying metabolic pathways and the influence of physical and biochemical factors on the metabolism of M. lepraemurium in vitro.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000096:biosynthesis; D002250:Carbon Radioisotopes; D005260:Female; D005615:Freezing; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D008099:Liver / Q000382:microbiology; D051379:Mice; D008815:Mice, Inbred Strains; D009167:Mycobacterium lepraemurium / Q000378:metabolism*; D009829:Oleic Acids / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D011773:Pyruvates / Q000378:metabolism
D000085:Acetates; D002250:Carbon Radioisotopes; D009829:Oleic Acids; D011136:Polysorbates; D011773:Pyruvates; D002245:Carbon Dioxide; D005947:Glucose; D005990:Glycerol; D005998:Glycine