Polarisation fluoroimmunoassay of gentamicin.
A polarisation fluoroimmunoassay has been developed for the routine determination of gentamicin levels in serum. The method employs fluorescein-labelled gentamicin which is easily, reproducibly and economically prepared and has excellent shelf-life. The assay is fast (minimum incubation time 2 min) and requires 1.25 mul of serum. There is no separation step prior to readout of results. Recovery experiments reveal no serum effects. Intra- and inter-assay coefficients of variation are of the order of 10% or less. Estimations of gentamicin levels in patient serum samples correlate closely with those obtained by bioassay (r = 0.93) and radioimmunoassy (r = 0.97).
D001681:Biological Assay; D005069:Evaluation Studies as Topic; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005839:Gentamicins / Q000097:blood*; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods
D005452:Fluoresceins; D005839:Gentamicins