Immunofluorescent localization of secretin in pancreatic monolayer culture.
Immunofluorescent cells to synthetic secretin were identified in monolayer culture of neonatal rat pancreas. No cross reaction of anti-secretin was observed with either glucagon, somatostatin or gastrin. The presence of cells containing secretin or a secretin-like peptide adds a new cell type to the three already characterized (insulin, glucagon and somatostatin containing cells) in monolayer culture.
D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000032:analysis* / Q000166:cytology; D051381:Rats; D012633:Secretin / Q000032:analysis*
D012633:Secretin