Identification of gonadotropic cells in the human pituitary by immunoperoxidase technique.
Using specific antibodies against the beta-subunits of human LH and FSH, the pituitary cells which produce the two gonadotropins have been localized in the human pituitary by an immunoperoxidase technique. In serial semi-thin sections (2 mum), it was clearly shown that about 90% of gonadotropic cells contained both FSH and LH, whereas the remaining 10% of gonadotrophs were positive only for FSH. At the ultrastructural level, the gonadotrophs containing both LH and FSH were characterized by the presen-e of two types of secretory granules: the smaller ones which whowed a strong immunohistochemical reaction and the larger ones which generally presented a weaker reaction.
D000906:Antibodies; D005640:Follicle Stimulating Hormone / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007986:Luteinizing Hormone / Q000096:biosynthesis* / Q000276:immunology; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism
D000906:Antibodies; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone