[Messenger RNA metabolism in E. coli under amino acid starvation conditions].
In E. coli Hfr Kavalli (met-) turnover of mRNA was studied in normal state and in methionine deficiency. This strain of E. coli was shown to have the 'loose' type of regulation of RNA synthesis (RC-). Without methionine in E. coli Hfr the rate of the protein synthesis was about 7-8% of that found in the control culture. In studies of the total mRNA turnover the half-life of matrices was equal to 2 min at 37degree, if a medium did not contain methionine; the same value was observed in the control culture. At the same time in methionine starvation an increase in amount of rapidly labelled RNA was observed; the RNA remained stable under simultaneous effect of actinomycin D. This rapidly labelled, stable RNA was the messenger-RNA, associated with cytoplasmic membranes. The hypothesis is advanced that membrane-associated and cytoplasmic RNA are those two types of matrices, which differed in their stability when the protein synthesis was inhibited.
D000596:Amino Acids / Q000172:deficiency*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002462:Cell Membrane / Q000378:metabolism; D003470:Culture Media; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D008715:Methionine / Q000172:deficiency; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D003470:Culture Media; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D008715:Methionine
Obmen informatsionnoĭ RNK u E. coli v usloviiakh aminokislotnogo golodaniia