[Kinetic study of the in vitro penetration and metabolism of cholesterol sulfate in the human placenta : demonstration of a direct formation of pregnenolone sulfate].
The direct metabolism of cholesteryl-sulfate into pregnenolone sulfate is pointed out during in vitro perfusion of the whole organ and incubation of slices. The metabolite so formed represents a significant fraction of the recovered radioactivity. Cholesteryl-sulfate, which may be hydrolyzed into cholesterol, as well, is considered as a possible precursor of placental steroidogenesis and particularly of progesterone. Differences in the cellular permeability depending on the experimental model considered appear again during these experiments.
D002463:Cell Membrane Permeability; D002784:Cholesterol / Q000031:analogs & derivatives*; D002788:Cholesterol Esters / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D010477:Perfusion; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011284:Pregnenolone / Q000096:biosynthesis*; D011374:Progesterone / Q000096:biosynthesis; D013431:Sulfates
D002788:Cholesterol Esters; D013431:Sulfates; D011374:Progesterone; D011284:Pregnenolone; D002784:Cholesterol
Etude cinétique de la pénétration et du métabolisme du sulfate de cholestérol dans le placenta humain in vitro : mise en évidence d'une formation directe de sulfate de prégnènolone