Comparative studies of sheep immunoglobulins IgG1 and IgG2: amino acid sequence of carboxy-terminal cyanogen bromide fragments from their heavy chains.
Sheep immunoglobulin IgG1 and immunoglobulin IgG2 heavy chains were treated with cyanogen bromide. The fractions from the C-terminal end of the heavy chains were isolated and purified, and the amino acid sequences gamma1 and gamma2 heavy chains had the identical sequence: Met-His-Glx-Ala-Leu-His-Asx-His-Tyr-Thr-Glx-Lys-Ser-Ile-Ser-Lys-Pro-Pro-Gly. Comparison with the C-terminal peptides of other species, reported in the literature, suggests that the subclasses are the results of recent evolutionary processes. Residues at position 4 from the C-terminus may be phylogenetically related.
D000595:Amino Acid Sequence; D000818:Animals; D003488:Cyanogen Bromide; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G / Q000032:analysis*; D007143:Immunoglobulin Heavy Chains / Q000032:analysis*; D007142:Immunoglobulin gamma-Chains / Q000032:analysis*; D012756:Sheep / Q000276:immunology*
D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D003488:Cyanogen Bromide