Mitochondrial DNA of Tetrahymena pyriformis strain ST contains a long terminal duplication-inversion.
1. We have studied denatured Tetrahymena mtDNA by electron microscopy using the formamide technique. 2. After denaturation all DNA is single stranded, but within a few minutes single-stranded circles with a duplex tail are formed. 3. The duplex tail is 1.3 mum long, i.e. 8 percent of the length of native mtDNA, and it often contains a small single-stranded eye. 4. Digestion of the duplex DNA with exonuclease III of Escherichia coli abolishes its ability to form circles and duplex tails after denaturation. 5. Renaturation of denatured mtDNA leads to the formation of duplex circles with single-stranded section and/or duplex tails. In addition, a minority of duplex circles without apparent tails is formed, but these circles contain a small ambiguous section. 6. We conclude that this mtDNA contains a long terminal duplication-inversion, that could be involved in the replication of this linear mtDNA.
D000818:Animals; D004270:DNA, Circular; D004272:DNA, Mitochondrial; D003851:Deoxyribonucleases; D004926:Escherichia coli / Q000201:enzymology; D005092:Exonucleases; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D013769:Tetrahymena pyriformis / Q000032:analysis*
D004270:DNA, Circular; D004272:DNA, Mitochondrial; D003851:Deoxyribonucleases; D005092:Exonucleases