The copper coordination group in "blue" copper proteins: evidence from resonance Raman spectra.
Tunable dye laser excitation in the intense similar to 600-nm absorption band of azurin, plastocyanin, and ceruloplasmin provides resonance enhanced Raman spectra. They consist of a complex set of bands, at least three or four in number, between 350 and 473 cm-1, which are assignable to Cu-N or Cu-O bond stretching, and a weak band near 270 cm-1, which probably arises from Cu-S stretching. A weak band at 765 cm-1 found in plastocyanin may arise from C-S stretching. Analysis of the Raman intensity pattern, as well as of the nature of the resonant electronic transition, leads to a model of the "blue" copper site involving approximately trigonal-bipyramidal coordination, with a sulfur and two nitrogen ligands in the equatorial plane, and less strongly bound nitrogen or oxygen ligands at axial positions. This arrangement would be well poised for stabilization of Cu(I) upon reduction.
D001400:Azurin; D001426:Bacterial Proteins; D002570:Ceruloplasmin; D003300:Copper; D007834:Lasers; D008024:Ligands; D008956:Models, Chemical; D009584:Nitrogen; D010940:Plant Proteins; D010970:Plastocyanin; D011550:Pseudomonas aeruginosa / Q000032:analysis; D012542:Scattering, Radiation; D013057:Spectrum Analysis; D013455:Sulfur
D001426:Bacterial Proteins; D008024:Ligands; D010940:Plant Proteins; D001400:Azurin; D013455:Sulfur; D003300:Copper; D010970:Plastocyanin; D002570:Ceruloplasmin; D009584:Nitrogen