Glycoprotein glycosyltransferases in male reproductive organs and their hormonal regulation.
The prostate glands of rats, mice, guinea pigs and hamsters were found to be a rich source of enzymes catalyzing the Mn2+-dependent transfer of galactose from UDP-galactose to glycoprotein acceptors such as ovomucoid and ovalbumin. The ventral prostate was also very active in promoting transfer of fucose from GDP-fucose to ovomucoid. The prostatic enzymes promoting both galactosyl and fucosyl transfers to glycoproteins were very largely membrane-bound, and were markedly activated by the non-ionic detergent Triton X-100. Castration of adult male resulted in a many-fold and roughly parallel decline in both glycosyltransferase activities over a period of two weeks, which was reversed by subsequent daily treatment with testosterone for 8 days. The very low galactosyltransferase of the ventral prostate of hypophysectomized rats was markedly enhanced by testosterone administration, whereas prolactin alone or in combination with androgen had no significant effect.
D000818:Animals; D002369:Castration; D006224:Cricetinae; D005260:Female; D005700:Galactosyltransferases / Q000378:metabolism*; D005837:Genitalia, Male / Q000201:enzymology*; D006154:Guanosine Diphosphate Fucose / Q000378:metabolism; D006168:Guinea Pigs; D006602:Hexosyltransferases / Q000378:metabolism*; D007016:Hypophysectomy; D007774:Lactation; D008297:Male; D008321:Mammary Glands, Animal / Q000201:enzymology; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D009929:Organ Size; D009928:Organ Specificity; D011247:Pregnancy; D011388:Prolactin / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D013739:Testosterone / Q000494:pharmacology*; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase / Q000378:metabolism*
D006154:Guanosine Diphosphate Fucose; D013739:Testosterone; D008345:Manganese; D011388:Prolactin; D005700:Galactosyltransferases; D006602:Hexosyltransferases; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase