Two improved methods for preparing ferritin-protein conjugates for electron microscopy.
Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin-antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.
D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D002234:Carbodiimides; D002850:Chromatography, Gel; D005293:Ferritins; D005976:Glutaral; D006041:Goats / Q000276:immunology; D007120:Immunochemistry; D007122:Immunoelectrophoresis; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007158:Immunologic Techniques / Q000592:standards; D007202:Indicators and Reagents; D007454:Iodides; D007457:Iodine Radioisotopes; D008854:Microscopy, Electron; D011817:Rabbits / Q000276:immunology; D013388:Succinimides
D000906:Antibodies; D002234:Carbodiimides; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007454:Iodides; D007457:Iodine Radioisotopes; D013388:Succinimides; D005293:Ferritins; D005976:Glutaral