[Isolation of active gonadotropin fractions from sturgeon hypophysis and procurement of specific immune sera to them].
The proteins of sturgeon hypophysis form 12 fractions when being separated by disc-electrophoresis. The maturation of the Rana esculenta or Bufo viridis oocytes in vitro served as a criterion of gonadotropic activity of fractions. The gonadotropic activity was shown to reside in two glycoprotein fractions with the mobilities 0.26 and 0.34. The immune serum to the former fraction is monospecific under immunodiffusion and suppresses the oocyte maturation in vitro. The immune serum to the latter fraction is characterized by the antigonadotropic activity as well, but expressed to a lesser extent.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D005399:Fishes / Q000378:metabolism*; D006023:Glycoproteins / Q000494:pharmacology; D006065:Gonadotropins, Pituitary / Q000302:isolation & purification*; D007106:Immune Sera; D005779:Immunodiffusion; D009865:Oocytes / Q000187:drug effects; D010902:Pituitary Gland / Q000032:analysis*
D006023:Glycoproteins; D006065:Gonadotropins, Pituitary; D007106:Immune Sera
Vydelenie aktivnykh fraktsiĭ gonadotropina iz gipofiza osetra i poluchenie k nim spetsificheskikh immunykh syvorotok