Simple disk-plate method for the biochemical confirmation of pathogenic Neisseria.
The carbohydrate fermentation test in cystine trypticase agar (BBL)-tubed medium was compared with a simple method using commercially available carbohydrate impregnated disks on culture inoculated Thayer-Martin medium with and without (vancomycin, colistimethate, and nystatin) inhibitors. There was 100% agreement between the two methods when a limited sample of clinical isolates of Neisseria gonorrhoeae and Nesseria meningitidis were tested.
D050260:Carbohydrate Metabolism; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D006069:Gonorrhea / Q000175:diagnosis; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification* / Q000378:metabolism; D009345:Neisseria meningitidis / Q000302:isolation & purification* / Q000378:metabolism