Genetic variation in the purine nucleoside phosphorylase activity of sheep red cells.
The purine nucleoside phosphorylase (NP) activity of sheep red cells was determined by starch gel electrophoresis and by a spectrophotometric assay technique. Some sheep had high activity (NP-high type) and some had low or zero activity (NP-low type). The enzyme deficiency is apparently confined to the red cell since other tissues from NP-low type animals had activities similar to those from NP-high type individuals. Family data indicated that NP activity is controlled by a pair of autosomal allelic genes, designated NPH and NPL. Sheep heterozygous for the NP genes had lower enzymic activities than homozygous high-type individuals. The frequency of NP types in different breeds of sheep was determined. Barbary and Mouflon sheep had activities similar to NP-high type domestic sheep; goats had high enzyme activities but their NP had a slower electrophoretic mobility than that of sheep.
D000818:Animals; D004912:Erythrocytes / Q000201:enzymology*; D005796:Genes; D014644:Genetic Variation; D006041:Goats; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D007908:Lens, Crystalline / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D010430:Pentosyltransferases / Q000097:blood*; D011683:Purine-Nucleoside Phosphorylase / Q000097:blood* / Q000172:deficiency; D012756:Sheep; D013045:Species Specificity; D013154:Spleen / Q000201:enzymology
D010430:Pentosyltransferases; D011683:Purine-Nucleoside Phosphorylase