Counterimmunoelectrophoresis as a routine mycoserological procedure.
Counterimmunoelectrophoresis (CIE) has been compared in a diangostic laboratory with agar gel double diffusion (DD) as a routine procedure for detection of antibodies to pathogenic and allergenic fungi and actinomycetes. It was shown to be of particular value in detecting antibodies to Aspergillus fumigatus. Thus 72 of 106 sera in which precipitins were detected were positive by CIE alone. Some sera were positive only by CIE to antigens prepared from Histoplasma capsulatum, Allescheria boydii, Candida albicans and C. parapsilosis.
D000192:Actinomycetales / Q000276:immunology; D000907:Antibodies, Bacterial / Q000032:analysis*; D000908:Antibodies, Fungal / Q000032:analysis*; D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D001203:Ascomycota / Q000276:immunology; D001230:Aspergillus / Q000276:immunology; D001232:Aspergillus fumigatus / Q000276:immunology; D002175:Candida / Q000276:immunology; D002176:Candida albicans / Q000276:immunology; D003378:Counterimmunoelectrophoresis / Q000379:methods*; D005203:Farmer's Lung / Q000276:immunology; D006658:Histoplasma / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis / Q000379:methods*; D008848:Micromonosporaceae / Q000276:immunology
D000907:Antibodies, Bacterial; D000908:Antibodies, Fungal; D000942:Antigens, Bacterial; D000946:Antigens, Fungal