Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test.
We have developed a rapid and simple indirect enzyme-labeled antibody procedure suitable for screening for antibodies to viral, bacterial, and parasitic antigens. Disposable microplates were utilized as antigen carriers and test vehicles. The test worked equally well with serum or whole unclotted blood. Using an automatic pipettor, 96 tests could be performed in 30 to 60 min. We report on the development of enzyme-labeled antibody procedures for the detection and surveillance of animal diseases.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000907:Antibodies, Bacterial / Q000032:analysis; D000914:Antibodies, Viral / Q000032:analysis; D002003:Brucella abortus / Q000276:immunology; D006691:Classical Swine Fever / Q000382:microbiology; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D006735:Horseradish Peroxidase / Q000276:immunology; D012328:RNA Viruses / Q000276:immunology; D012698:Serologic Tests / Q000379:methods*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D014234:Trichinella / Q000276:immunology
D000906:Antibodies; D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D006735:Horseradish Peroxidase