Chloride transport across isolated opercular epithelium of killifish: a membrane rich in chloride cells.
The opercular epithelium of Fundulus heteroclitus contains typical gill chloride-secreting cells at the high density of 4 X 10(5) cells per square centimeter. When isolated, mounted as a membrane, and short-circuited, it actively transports chloride ions from the blood side to the seawater side of the preparation. This preparation offers a useful approach to the study of osmoregulation in bony fishes.
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism*; D005399:Fishes / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology; D005880:Gills; D066298:In Vitro Techniques; D007695:Killifishes / Q000378:metabolism*; D008564:Membrane Potentials; D010042:Ouabain / Q000494:pharmacology; D013861:Thiocyanates / Q000494:pharmacology; D014882:Water-Electrolyte Balance
D001639:Bicarbonates; D002712:Chlorides; D013861:Thiocyanates; D010042:Ouabain; D005665:Furosemide