title: The effect of delaying incubation in a C02-enriched environment on gonococci.
abstract: Neisseria gonorrhoeae were isolated from 1,019 (39.8%) of 2,558 male patients examined in a venereal disease clinic. Three specimens per patient were plated on Thayer-Martin medium. One plate was placed in a C02 -enriched environment and incubated immediately. The other two were held at room temperature in a standard atmosphere for 1-4 hours before similar processing. A statistically significant loss of N. gonorrhoeae was found when placement in a candle jar and incubation was delayed 2 or more hours. Morever, the appearance of colonies was delayed and colonial size and number were significantly reduced in specimens held 2 hours or longer before incubation in a candle jar.
mesh_terms: D001431:Bacteriological Techniques; D002245:Carbon Dioxide; D006069:Gonorrhea / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D008297:Male; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification*; D013048:Specimen Handling; D013997:Time Factors
chemical_list: D002245:Carbon Dioxide