Characterization of ineffective erythropoiesis in erythroleukaemia.
In 6 cases initially showing a symptomatology of erythroleukemia, the proliferation of erythroblasts was studied by combined Feulgen cytophotometry and autoradiography after labelling with 3H-TdR in vivo.  A proliferation disturbance was observed consisting of an accumulation of diploid and unlabelled cells and a decreased proportion of cells in S.  This defect occurred within the basophilic cell compartment in one case and within the early polychromatic cell compartment in all 6 cases.  The results indicate the existence of out-of-cycle cells, which may be responsible for the inefficacy for erythropoiesis in this disease.  The defect was present in all 4 cases with acute erythroleukaemia.  In one of the two cases showing no deterioration of the physical and haematological condition over a period of years, an additional defect was observed, consisting of a striking accumulation of tetraploid unlabelled cells.  Therefore, the technique used may be suitable as a diagnostic tool for evaluation of new types of ineffective erythropoiesis.
D000368:Aged; D001491:Basophils / Q000378:metabolism; D001772:Blood Cell Count; D004247:DNA / Q000096:biosynthesis / Q000097:blood; D004171:Diploidy; D004900:Erythroblasts / Q000378:metabolism; D004920:Erythropoiesis; D005260:Female; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D011123:Polyploidy
D004247:DNA