title: Thermal denaturation of B. subtilis DNA in H2O and D2O observed by electron microscopy.
abstract: In the present work we studied the initial part of thermal denaturation curves of B. subtilis DNA in normal and heavy water, observing, by electron microscopy, the "opening" of DNA as a function of temperature. The results support the hypothesis that D(2)O plays a stabilizing role on strands separation during thermal denaturation of DNA.
mesh_terms: D001412:Bacillus subtilis / Q000032:analysis*; D001665:Binding Sites; D004269:DNA, Bacterial / Q000302:isolation & purification; D003903:Deuterium; D006358:Hot Temperature; D008854:Microscopy, Electron; D009691:Nucleic Acid Denaturation; D014867:Water
chemical_list: D004269:DNA, Bacterial; D014867:Water; D003903:Deuterium