Evidence for energy migration from photosystem I to photosystem II and the effect of magnesium.
1. With Euglena chloroplasts 7722 nm light excites a fluorescence induction pattern, measured at l,u nm, typical of the Photosystem II reduction of Q. 2. Both methylviologen and phenazine methosulfate partially quench this fluorescence rise in the absence of electron transport between Photosystem II and Photosystem I, and in a manner consistent with their known role of stimulating Photosystem I photochemistry. 3. The variable fluorescence excited with 22 nm light is reduced to a greater extent than that excited by 638 nm light upon chlorophyll dilution during division in the dark. 4. These observations are interpreted to indicate that in Euglena chloroplasts light absorbed by Photosystem I is transferred energetically "uphill" to Photosystem II, where it can perform Photosystem II photochemistry. 5. Magnesium ions stimulate the variable fluorescence with both 638 nm and 722 nm light to a similar extent, which argues against the concept of magnesium ion interrupting energy transfer between Photosystem II and Photosystem I.
D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D004237:Diuron / Q000494:pharmacology; D005056:Euglena gracilis / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D008274:Magnesium / Q000494:pharmacology*; D010269:Paraquat / Q000494:pharmacology; D010619:Phenazines / Q000494:pharmacology; D010785:Photophosphorylation / Q000187:drug effects; D013050:Spectrometry, Fluorescence
D010619:Phenazines; D002734:Chlorophyll; D004237:Diuron; D008274:Magnesium; D010269:Paraquat