title: An investigation concerning tumor cell antigenicity of hamster malignant melanoma utilizing heterologous antiserum.
abstract: The antigenic potential of spontaneous malignant melanoma of the Syrian hamster was investigated. Antisera to the tumor were prepared in two groups of rabbits; one group was immunized with tumor homogenate material and the other with tissue cultured tumor cells. The antiserum was absorbed with normal hamster tissue extracts and serum to remove cross-reacting antibodies. The presence of tumor specific antibodies was demonstrated using immunodiffusion and fluorescent antibody staining techniques. Tumor specific antiserum prepared in this manner gave a single precipitin line against tumor tissue in gel-diffusion tests, and showed specific staining of tumor cells in the fluorescent antibody test.
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D006224:Cricetinae; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008545:Melanoma / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology*; D011817:Rabbits / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D007106:Immune Sera