Ferredoxin biosynthesis in Euglena gracilis.
Analysis of ferredoxin content in cultures of Euglena gracilis grown in the presence of selective antibiotic inhibitors of protein synthesis resulted in the following conclusions: 1. Ferredoxin is synthesized from cytoplasmic (80s-type) ribosomes; cycloheximide, a potent inhibitor of 80s translation completely abolished the synthesis, while the inhibitors of 70s translation chloramphenicol and erythromycin were not effective. In addition, ferredoxin was detected in a streptomycin-bleached mutant that lacks the chloroplast structure and chloroplast DNA. 2. Ferredoxin's transcript is presumably of nuclear origin; rifampicin, an inhibitor of chloroplast DNA-dependent RNA polymerase did not inhibit synthesis, while the streptomycin-bleached mutant continued to synthesize ferredoxin without chloroplast DNA.
D000818:Animals; D001693:Biological Transport, Active; D002455:Cell Division / Q000187:drug effects; D002701:Chloramphenicol / Q000378:metabolism / Q000494:pharmacology; D002734:Chlorophyll / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D004917:Erythromycin / Q000494:pharmacology; D005056:Euglena gracilis / Q000187:drug effects / Q000378:metabolism*; D005288:Ferredoxins / Q000096:biosynthesis*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology
D005288:Ferredoxins; D002734:Chlorophyll; D004917:Erythromycin; D002701:Chloramphenicol; D003513:Cycloheximide; D007501:Iron; D005954:Glucosephosphate Dehydrogenase; D012293:Rifampin