[Study by fluorescence of DNA treated by 7-methyl-benzo (a) anthracene in the presence of a microsomal oxidation system].
When calf thymus DNA is treated with 7 methyl benzo(a)anthracene is presence of NADPH requiring microsomal hydroxylating system, the fluorescent signal observed on DNA after purification of the macromolecule shows the presence of 2 kinds of the hydrocarbon metabolites. One would countain probably a conjugated system analogous to the parent hydrocarbon ones, the other corresponds to a reduced conjugated system.
D000818:Animals; D001551:Benz(a)Anthracenes / Q000378:metabolism*; D001711:Biotransformation; D002417:Cattle; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence; D013950:Thymus Gland
D001551:Benz(a)Anthracenes; D004247:DNA
Etude par fluorescence d'ADN traite par le méthyl 7 benzo(a) anthracène en présence d'un système microsomal d'oxydation