Identification and separation of components of calf thymus DNA using a CsC1-netropsin density gradient.
Calf thymus DNA containing satellite components of various densities was used as a model to study the effect of netropsin on the density of DNA in a CsCl gradient. The binding of netropsin resulted in a decrease in density which depended upon the quantity of netropsin added and on the average composition of the DNA. Differences in density of DNA components were higher in CsCl - netropsin  gradients than in simple CsCl gradients. By use of netropsin a main band and four satellite bands could be  differentiated in calf thymus DNA. Satellite DNA's were isolated using preparativeCsCl - netropsin gradient centrifugation and were characterised by density and homogeneity in native and in reassociated state. Two of the satellite components, with densities of 1.722 and 1.714 g/cm minus 3, are probably of homogenous sequence, the other two components of densities 1.709 and 1.705 g/cm minus 3 appear to be heterogeneous.
D000818:Animals; D000900:Anti-Bacterial Agents; D001665:Binding Sites; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002586:Cesium; D004247:DNA / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000032:analysis; D008722:Methods; D010455:Peptides; D011485:Protein Binding; D013950:Thymus Gland / Q000032:analysis*
D000900:Anti-Bacterial Agents; D010455:Peptides; D002586:Cesium; D004247:DNA