[Comparative studies of two alphavirus by macroplaque assays and by a fluorescent focus method in Vero cell lines (author's transl)].
Immune anti-Sindbis and anti-Chikungunya ascitic fluids have been titrated by the classic way of plaque reduction and by a quick fluorescent focus method using an indirect staining. The results are comparable, allowing to consider the use of this quick and inexpensive technic for counting-assays by neutralization.
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D002460:Cell Line; D002646:Chikungunya virus / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D007668:Kidney; D012845:Sindbis Virus / Q000302:isolation & purification*; D010948:Viral Plaque Assay
Etude comparative de deux alphavirus par la méthode des plages et par comptage des foyers fluorescents sur cellules Véro