title: Effects of some chemical factors on flagellation and swarming of Vibrio alginolyticus.
abstract: Vibrio alginolyticus strains recently isolated from Dutch coastal seawater changed flagellar organization when cultivated in the presence of certain chemical agents. On agar media with more than 4.0% (w/v) NaCl the number of lateral flagella per cell decreased with increasing salt concentration. Both on agar media and in broth cultures with 6.0-9.0% (w/v) NaCl, cells with polar tufts of 2-4 sheathed or unsheathed flagella were frequently found. Cells grown on agar media with 7.3-9.8% (w/v) Na2SO4 had drastically reduced numbers of lateral flagella, but lacked polar tufts. EDTA suppressed growth, but did not affect flagellar arrangement. In the presence of 0.1-0.3% boric acid or 0.05-0.1% aluminum hydroxide, cells in liquid media tended to produce lateral, in addition to the polar flagella normally observed in broth cultures. Of a number of surface-active agents tested, Tween 80 and Na-taurocholate, even in high concentrations, did not affect flagellation. Bile salts (0.1%) and Na deoxycholate (0.05%) strongly reduced the number of both polar and lateral flagella. In agar cultures, Na-lauryl sulphate (0.01-0.1%) inhibited the formation of lateral, but increased the incidence of polar flagella. Teepol (0.05-0.2%) had a similar effect and also it had a deteriorating effect on the sheaths of the polar flagella. Concomitant with the reduction in the number of lateral flagella, induced by these agents, swarming on agar media was inhibited.
mesh_terms: D000362:Agar / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*; D014733:Vibrio / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D003470:Culture Media; D013501:Surface-Active Agents; D012965:Sodium Chloride; D000362:Agar; D004492:Edetic Acid; D012964:Sodium; D008274:Magnesium; D002118:Calcium