The hydrolysis of human IgG with subtilisin.
Small amounts of subtilisin (0.1 mg. per ml.) in the absence of cysteine will instantaneously hydrolyze "enzyme-sensitive" IgG globulins. This procedure permits the identification of antibodies in whole serum and myeloma globulins in the ultracentrifuge with simplicity and speed unmatched by other technics.
D001963:Bromelains; D002918:Chymotrypsin; D006801:Humans; D006868:Hydrolysis; D007074:Immunoglobulin G; D009194:Myeloma Proteins / Q000302:isolation & purification*; D010196:Pancreatic Elastase; D010206:Papain; D012204:Rh-Hr Blood-Group System; D013381:Subtilisins; D014357:Trypsin
D007074:Immunoglobulin G; D009194:Myeloma Proteins; D012204:Rh-Hr Blood-Group System; D001963:Bromelains; D013381:Subtilisins; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin; D010206:Papain