title: The mitochondrial genome of Euglena gracilis.
abstract: Mitochondrial DNA from Euglena gracilis has been investigated in its chemical and physical properties. Its G + C content is equal to 25%; its buoyant density in a CsCl density gradient (1.690 g/cm3) is higher, by 5 mg/cm3, than expected for a bacterial DNA having the same base composition. The buoyant densities of denatured and renatured DNA are higher than that of native DNA by 10-12 mg/cm3 and 6 mg/cm3, respectively. The melting temperature, Tm, is 77 degrees C in standard saline citrate; the first derivative of the melting curve shows a striking multimodality. Degradation of the DNA by micrococcal nuclease indicates that about 40% of the DNA is formed by stretches lower than 10% in G + C. In all its properties the mitochondrial DNA from Euglena gracilis is strikingly similar to that of Saccharomyces cerevisiae.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D003596:Cytosine / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis; D005056:Euglena gracilis / Q000032:analysis*; D005796:Genes; D006147:Guanine / Q000032:analysis; D006358:Hot Temperature; D007700:Kinetics; D008836:Micrococcal Nuclease; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation
chemical_list: D004272:DNA, Mitochondrial; D006147:Guanine; D003596:Cytosine; D008836:Micrococcal Nuclease