title: The oral apparatus of Tetrahymena pyriformis, strain WH-6. II. Cytochalasin B inhibition of oral apparatus morphogenesis.
abstract: The effects of cytochalasin B on oral apparatus morphogenesis and cell division were studied in synchronized Tetrahymena pyriformis, strain WH-6 syngen 1. Cytochalasin B brought about the rapid arrest of oral apparatus primordium development when added prior to the completion of oral apparatus membranelle differentiation. Cells arrested in development did not divide. When cytochalasin B was added after this transition point, oral apparatus morphogenesis and cell division were completed. The effects of cytochalasin B could be reversed by washing it from the medium. Even though cytochalasin B (at 400 mug/ml) reduced protein synthesis by 30%, the data are consistent with the interpretation that cytochalasin B prevents an assembly process during the membranelle differentiation phase of oral apparatus development.
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology*; D009024:Morphogenesis / Q000187:drug effects*; D013769:Tetrahymena pyriformis / Q000166:cytology / Q000187:drug effects*
chemical_list: D003571:Cytochalasin B