Purification and properties of tuberculin-active protein from Mycobacterium tuberculosis.
When Mycobacterium tuberculosis was grown on Sauton medium, intracellular tuberculin-active protein was produced. This product was purified by chromatography on DEAE-cellulose and Sephadex G-200 and was obtained in crystalline form. The crystals were plates, somewhat irregular in shape, about 50 mum in length and 25 mum in width. The yield corresponded to a 1.5% over-all recovery of total protein. Ultracentrifugal analysis showed only one major component with a calculated molecular weight of 9700. Sedimentation velocity analysis gave a sedimentation coefficient at 20 degrees (see article) of 1.73 S. The estimated specific activities of tuberculin-active protein were 6.33 times 10-9 tuberculin units per mg of protein-nitrogen for sensitized guinea pigs and 6.33 times 10-11 tuberculin units per mg of protein-nitrogen for humans. This is the most potent tuberculin-active protein that has yet been obtained.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001500:BCG Vaccine; D001426:Bacterial Proteins / Q000302:isolation & purification; D001681:Biological Assay; D002241:Carbohydrates / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D003460:Crystallization; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006168:Guinea Pigs / Q000276:immunology; D006801:Humans; D008970:Molecular Weight; D009163:Mycobacterium bovis; D009169:Mycobacterium tuberculosis / Q000032:analysis*; D012867:Skin / Q000276:immunology; D014373:Tuberculin / Q000302:isolation & purification; D014461:Ultracentrifugation
D000596:Amino Acids; D001500:BCG Vaccine; D001426:Bacterial Proteins; D002241:Carbohydrates; D014373:Tuberculin; D005688:Galactosamine; D005944:Glucosamine