A preserved leukocyte preparation for quality control.
A preparation technic for stabilized leukocytes for quality control is described. Human leukocytes are separated from 5 ml. of blood using dextran; the erythrocytes are lysed with isotonic NH4Cl-Tris buffer, pH 7.2. The washed leukocytes are resuspended in 50 ml. of cacodylate HCl buffer, pH 7.4. Five ml. of 2.5% glutaraldehyde were slowly added, and preservation is accomplished by storage overnight at 4 C. The washed leukocytes are resuspended in physiologic saline solution containing 1:1,000 sodium azide, then stored at 4 C and at room temperature. The preparations were highly stable at both temperatures for at least 3 months, as indicated by both manjal and electronic leukocyte counts.
D001386:Azides; D001793:Blood Preservation / Q000379:methods*; D002021:Buffers; D003911:Dextrans; D005976:Glutaral; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes; D008853:Microscopy; D011786:Quality Control
D001386:Azides; D002021:Buffers; D003911:Dextrans; D005976:Glutaral