Simple procedure for production by group C streptococci of phage-associated lysin active against group A streptococci.
Phage-associated lysin of high potency was prepared by growing the host group C streptococcal strain 26RP66 in a semisynthetic medium. The lysin was stabilized by adding dithiothreitol and neutralized ethylenediaminetetraacetic acid (EDTA) to facilitate further concentration and partial purification. The lysin remained active when stored at -65 C for 1 year. Lysin was active against all strains of group A streptococci tested and was more active against living cells than heat-killed cells. The procedure outlined is practicable for most bacteriological research laboratories and does not require column purification or other complex biochemical procedures. It should be useful to any laboratory which requires small amounts of lysin to produce L-forms and protoplasts or to release streptococcal antigens.
D000645:Ammonium Sulfate; D001433:Bacteriolysis; D001435:Bacteriophages / Q000201:enzymology*; D011232:Chemical Precipitation; D004229:Dithiothreitol; D004355:Drug Stability; D004492:Edetic Acid; D004798:Enzymes / Q000302:isolation & purification; D013045:Species Specificity; D013291:Streptococcus / Q000187:drug effects; D013297:Streptococcus pyogenes / Q000187:drug effects; D014461:Ultracentrifugation
D004798:Enzymes; D004492:Edetic Acid; D000645:Ammonium Sulfate; D004229:Dithiothreitol