title: Equine anti-hapten antibody. IX. IgM anti-lactose antibodies.
abstract: The immune response to a bacterial vaccine of Streptococcus faecalis (strain N) was characterized in all of the seven horses studied by the sustained production of about 90% IgM anti-lactose antibody over a period of 44 weeks with maximum values of the total antibody ranging from 4 mg/ml of serum to 12 mg/ml of serum. With respect to the binding of a lactose-containing ligand the association constants of the antibodies purified from sera obtained between 5 and 44 weeks fell in the range of 1 times 10-5 M-1 to 2 times 10-5 M-1. Not only was there no significant indication of maturation of a-finity in this period but there was a selective limitation of affinity compared to that of 7S antibodies. It was inferred that the synthesis of IgM antibody involves the selective utilization of V-H and/or V-L genes.
mesh_terms: D000327:Adsorption; D000818:Animals; D000910:Antibodies, Heterophile / Q000032:analysis; D000917:Antibody Formation; D000918:Antibody Specificity; D000942:Antigens, Bacterial; D001391:Azo Compounds; D002250:Carbon Radioisotopes; D013293:Enterococcus faecalis / Q000276:immunology; D006027:Glycosides; D006241:Haptens; D006736:Horses / Q000276:immunology*; D007106:Immune Sera; D007115:Immunization Schedule; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007785:Lactose / Q000276:immunology*; D008024:Ligands; D011134:Polysaccharides; D014316:Tritium
chemical_list: D000910:Antibodies, Heterophile; D000942:Antigens, Bacterial; D001391:Azo Compounds; D002250:Carbon Radioisotopes; D006027:Glycosides; D006241:Haptens; D007106:Immune Sera; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D008024:Ligands; D011134:Polysaccharides; D014316:Tritium; D007785:Lactose