The effects of 6-hydroxydopamine on the appearance of granulated vesicles in glomus cells of the rat carotid body.
The glomus cells of the rat carotid body reveal an intense fluorescence after exposure to paraformaldehyde vapor and contain catecholamines. After initial fixation in glutaraldehyde, many granulated vesicles are seen in the glomus cells. After initial fixation in osmium tetroxide, most of the vesicles are depleted of their dense interiors and granulated vesicles occur infrequently. Administration of 6-hydroxydopamine followed by initial fixation in osmium tetroxide leads to the reapperance of dense interiors in virtually all vesicles. 6-Hydroxydopamine apparently is taken up by the membrane pump of the glomus cell and is incorporated into the amine storage granules, thereby displacing the endogenous monoamines. Osmium tetroxide does not dissolve the 6-hydroxydopamine from the vesicles, as it apparently does for the normal vesicular contents. The 6-hydroxydopamine does not fluoresce, hence 6-hydroxydopamine administration results in a decreased intensity of formaldehyde induced fluorescence in the glomus cells. Administration of reserpine after 6-hydroxydopamine treatment (and subsequent initial fixation in osmium tetroxide) depletes the previously restored dense material from the vesicles of the glomus cells. 6-Hydroxydopamine acts like a monoamine in that it is taken up by the glomus cell, incorporated into the vesicles, and can be depleted from the vesicles by reserpine.
D000818:Animals; D002344:Carotid Body / Q000187:drug effects* / Q000648:ultrastructure; D005453:Fluorescence; D005557:Formaldehyde; D005976:Glutaral; D006892:Hydroxydopamines / Q000494:pharmacology*; D009940:Organoids / Q000187:drug effects / Q000648:ultrastructure; D009992:Osmium; D051381:Rats; D012110:Reserpine / Q000494:pharmacology
D006892:Hydroxydopamines; D005557:Formaldehyde; D009992:Osmium; D012110:Reserpine; D005976:Glutaral