Comparative studies on the specificity of anticlastogenic action in human lymphocytes in culture.
Comparative studies on human lymphocyte cultures yielded a certain specificity of the anticlastogenic action of the SH compounds l-cysteine, cysteamine, and beta-aminoethylisothiouronium (AET) as well as of the amide l-asparagine and the amino acid l-methionine. This specific anticlastogenic activity manifested itself in specific changes of the spectrum of aberration types induced by the clastogens and of the pattern of intercellular distribution of the induced aberrations. It was clearly dependent on the concentration of the anticlastogens but was also influenced by the used clastogen. The use of different culture media yielded some quantitative influences on the anticlastogenic activity, but fundamental changes in the spectrum of anticlastogenic action have not been observed except with cysteamine. The patterns of activity ascertained for the different anticlastogens specifically differed from those changes in the spectrum and pattern of distribution of aberrations induced by a mere reduction of the concentration for instance of Trenimon. Therefore a direct reaction between the protectors and the clastogen Trenimon as the cause of the observed anticlastogenic action was again excluded. The presented data are also discussed under the aspects of the hypotheses of aberration induction as well as of their importance for further antimutagen research.
D001216:Asparagine / Q000494:pharmacology; D002066:Busulfan / Q000494:pharmacology; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D003470:Culture Media; D003543:Cysteamine / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008715:Methionine / Q000494:pharmacology; D009153:Mutagens / Q000037:antagonists & inhibitors; D015125:Oxyquinoline / Q000494:pharmacology; D014228:Triaziquone / Q000494:pharmacology; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology
D003470:Culture Media; D009153:Mutagens; D000338:beta-Aminoethyl Isothiourea; D015125:Oxyquinoline; D003543:Cysteamine; D001216:Asparagine; D008715:Methionine; D014228:Triaziquone; D002066:Busulfan; D003545:Cysteine