Competition between substrates for acetylcholinesterase and cholinesterase.
The kinetics of competition of pairs of two substrates for bovine erythrocyte acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) and horse serum cholinesterase (acylcholine acyl-hydrolase, EC 3.1.1.8) was studied so that the hydrolysis of only one substrate was measured at a time. The substrates were acetylthiocholine, were used as competiting substrates i.e. inhibitors. The substrate inhibition constants (Kss) and Michaelis constants for the reaction of a single substrate were also determined. It was concluded that the substrate inhibition site in the enzyme does not show up in the competition between two substrates.
D000109:Acetylcholine / Q000494:pharmacology; D000110:Acetylcholinesterase / Q000097:blood*; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002802:Cholinesterases / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology; D006736:Horses; D007700:Kinetics
D000110:Acetylcholinesterase; D002802:Cholinesterases; D000109:Acetylcholine