Protection of rats by phospholipase C from Bacillus cereus against the effects of intravenous infusions of purified tissue thromboplastin.
Infusions of purified tissue thromboplastin in rats cause the accumulation of fibrin and platelets in the lungs and produce marked changes in the platelet count and in the coagulation factors V, VII and VIII. Tissue thromboplastin in a dose corresponding to less than 2 mug of protein per rat is lethal when given as a bolus injection. Simultaneous i.v. administration purified phospholipase C effectively prevents all these changes and protects rats from otherwise lethal doses of tissue thromboplastin. The necessary doses of phospholipase C are well below the toxic level for phospholipase C alone.
D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D001772:Blood Cell Count; D001779:Blood Coagulation Factors / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects; D005337:Fibrin / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D007275:Injections, Intravenous; D008297:Male; D010740:Phospholipases / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D011652:Pulmonary Circulation; D051381:Rats; D013925:Thromboplastin / Q000008:administration & dosage / Q000633:toxicity; D013927:Thrombosis / Q000517:prevention & control*; D013997:Time Factors
D001779:Blood Coagulation Factors; D005337:Fibrin; D005340:Fibrinogen; D013925:Thromboplastin; D010740:Phospholipases