Immunochemical and functional similarities and differences among iron-sulfur proteins involved in mammalian steroidogenesis.
An antibody prepared against bovine adrenodoxin has been employed to study possible similarities or differences among the iron-sulfur protein components of electron transport sequences associated with cytochrome P-450 function in the mitochondria of mammalian steroidogenic tissues. Although they are not identical, the "adrenodoxins" in mitochondria from the adrenals of a number of species, including man, are both immunochemically and functionally similar. The ability of the antibody to inhibit adrenal 11 beta-hydroxylase and cholesterol side-chain cleavage activities demonstrates the requirement for adrenodoxin in these mixed-function oxidations. The results presented also demonstrate the immunochemical and functional similarities among the iron-sulfur proteins which are involved in the oxidative cleavage of the cholesterol side-chain occurring in adrenal, ovarian and testicular mitochondria. The iron-sulfur protein involved in cholesterol side-chain cleavage activity in term placental mitochondria, however, appears to be immunochemically different from these other proteins.
D000302:Adrenal Cortex / Q000276:immunology; D000311:Adrenal Glands / Q000276:immunology / Q000378:metabolism; D000325:Adrenodoxin / Q000276:immunology*; D000818:Animals; D002415:Cats; D002417:Cattle; D002784:Cholesterol / Q000378:metabolism; D004285:Dogs; D004579:Electron Transport; D005260:Female; D006041:Goats / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D005779:Immunodiffusion; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000276:immunology / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010053:Ovary / Q000276:immunology; D010920:Placenta / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D051381:Rats; D013256:Steroids / Q000096:biosynthesis*; D013737:Testis / Q000276:immunology
D013256:Steroids; D000325:Adrenodoxin; D002784:Cholesterol; D006899:Mixed Function Oxygenases; D009251:NADPH-Ferrihemoprotein Reductase