Lecithin agar for detection of microbial phospholipases.
Lecithin agar was developed on which phospholipase C produced turbid zones and phospholipase A produced clear zones. Reactions on lecithin agar agreed 74% of the time with reactions in egg yolk broth. On lecithin agar, interpretation was easier, phospholipase A was detectable, and opaque zones were visible 1 or 2 days earlier than on egg yolk agar. All constituents of the medium can be autoclaved.
D000362:Agar; D002118:Calcium / Q000494:pharmacology; D004530:Egg Yolk; D005260:Female; D010713:Phosphatidylcholines / Q000378:metabolism; D010740:Phospholipases / Q000096:biosynthesis* / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology*; D011551:Pseudomonas fluorescens / Q000201:enzymology
D010713:Phosphatidylcholines; D000362:Agar; D010740:Phospholipases; D002118:Calcium