[Inhibition of the incorporation of tritiated thymidine in fetal rat liver in cell culture by a fractioned liver extract].
A dialyzed rat liver extract has been chromatographed on "Sephadex G 25" and then on "Sephadex G 50". Thus we found a fraction which inhibits the incorporation of tritiated thymidine and stimulates the incorporation of labelled orotic acid into foetal rat liver cells in culture, but has no effect on DNA synthesis in cell lines derived from lymphocytes and fibroblasts.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis; D003956:Dialysis; D005347:Fibroblasts / Q000187:drug effects; D008099:Liver / Q000378:metabolism*; D008110:Liver Extracts / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects; D009963:Orotic Acid / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D008110:Liver Extracts; D009963:Orotic Acid; D012313:RNA; D004247:DNA; D013936:Thymidine
Inhibition de l'incorporation de thymidine tritéé dans le foie foetal de rat en culture cellulaire par un extrait hépatique fractionné