Animal model of Fusarium solani keratitis.
Because of the frequency of fungal keratitis due to Fusarium solani, we needed a sustained, progressive infection in an animal model to determine the mechanisms of pathogenicity and to evaluate the new antifungal agents. Pigmented rabbits interlamellarly injected with actively germinating conidia from lyophilized temperature-tolerant strains of F. solani produced sustained culture-positive ulcers in high percentage of eyes at two and three weeks, pretreatment with subconjunctival corticosteroids was necessary. Histopathology, although a poor index of infectivity since some corneas with plentiful hyphal fragments had negative cultures, simulated human fungal pathology.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D001232:Aspergillus fumigatus / Q000472:pathogenicity; D001431:Bacteriological Techniques; D003315:Cornea / Q000382:microbiology* / Q000473:pathology; D046508:Culture Techniques; D004195:Disease Models, Animal; D005670:Fusarium; D000882:Haplorhini; D007634:Keratitis / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology; D009181:Mycoses / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology; D009824:Ointments; D011239:Prednisolone / Q000008:administration & dosage / Q000627:therapeutic use; D011817:Rabbits; D014221:Triamcinolone / Q000008:administration & dosage / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D009824:Ointments; D014221:Triamcinolone; D011239:Prednisolone