Antibodies to an NH2-terminal fragment of betaS globin. I. Preparation and radioimmunoassay.
Polypeptide fragments corresponding to the NH2-terminal 55 amino acids of betaS and betaA globins were prepared by cyanogen bromide treatment of globin and isolated by gel filtration on Sephadex G-50. Sheep were immunized with the isolated NH2-terminal fragments, and one of these sheep produced precipitating antibodies to the NH2-terminal fragment of betaS globin. These antibodies also reacted with betaA and betaS globin and hemoglobins A and S, as shown by immunodiffusion and quantitative precipitation studies. A radioimmunoassay was developed using the radioiodinated NH2-terminal fragment as tracer, and dextran-coated charcoal for separating bound and free peptide. The radioimmunoassay was used to characterize the interaction of the antibodies and the NH2-terminal fragment of betaS globin.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies; D001665:Binding Sites; D001666:Binding Sites, Antibody; D005914:Globins / Q000276:immunology*; D006451:Hemoglobin, Sickle; D006455:Hemoglobins, Abnormal; D006801:Humans; D005779:Immunodiffusion; D007700:Kinetics; D011233:Precipitin Tests; D011485:Protein Binding; D012756:Sheep / Q000276:immunology
D000596:Amino Acids; D000906:Antibodies; D006451:Hemoglobin, Sickle; D006455:Hemoglobins, Abnormal; D005914:Globins