A general method for enumerating single cells secreting antigen: albumin-secreting hepatocytes detected as plaque-forming cells.
Sheep erythrocytes, artificially coated with purified antibody specific for mouse serum albumin (anti-MSA Ab-E) were agglutinated and lysed by mouse serum albumin. Mouse liver cells, plated with anti-MSA Ab-E formed hemolytic plaque in a modified plaque-forming cell (PFC) assay. The same cells tested with anti-MSA Ab-E did not rosette in modified immunocyto-adhesion assay. Thus, liver cells secreted molecules (albumin) which were not detectable on the cell membrane. This PFC assay with Ab-E is probably a general method for enumerating antigen-secreting cell.
D000371:Agglutination; D000818:Animals; D000906:Antibodies; D000941:Antigens; D004912:Erythrocytes / Q000276:immunology; D006461:Hemolysis; D006462:Hemolytic Plaque Technique; D008099:Liver / Q000166:cytology*; D051379:Mice; D012397:Rosette Formation; D012709:Serum Albumin / Q000276:immunology*; D012756:Sheep
D000906:Antibodies; D000941:Antigens; D012709:Serum Albumin