Extraction methods and test techniques for detection of vegetable proteins in meat products. I. Qualitative detection of soya derivatives.
Extracts of 3 soya bean preparations, used commercially in certain countries to replace part of the meat in popular meat products, were made by treatment with (i) sodium dodecyl sulphate, (ii) Triton-X100 or (iii) n-Butanol. Similar extracts were made from beef and pork. All extracts were examined by electrophoretic and immunological techniques. Stained polyacrylamide gels revealed distinctive protein bands after electrophoresis. The migration rates of corresponding bands differed between beef and pork extracts. However, the migration rates of vegetable bands revealed certain similarities, but differed very greatly from those of animal origin. Characteristic fast-migrating S-bands were distinguishable only in extracts of vegetable protein. Immunodiffusion tests, using antisera produced in rabbits against each extract, revealed varying degrees of similarity between extracts of vegetable origin, but the antisera were specific for either vegetable or animal protein.
D000818:Animals; D000440:Butanols; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D008460:Meat / Q000032:analysis*; D009124:Muscle Proteins / Q000032:analysis; D014674:Plant Proteins, Dietary / Q000032:analysis*; D011092:Polyethylene Glycols; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D013025:Glycine max / Q000032:analysis*; D013552:Swine
D000440:Butanols; D009124:Muscle Proteins; D014674:Plant Proteins, Dietary; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols