Studies on gonococcus infection. VIII. 125Iodine labeling of gonococci and studies on their in vitro interactions with eukaryotic cells.
Intact gonococci (GC) have been labeled with 125iodine by the lactoperoxidase plus hydrogen peroxide procedure. The specific activities of types 2, 4, and 4 GC have been determined and are found to show small differences as follows: T4 greater than T2 greater than T4. 125I-labeled GC have been studied for their associations with both leukocytes and tissue culture cells. 125I-labeled GCshow the following relative order of association with the leukocytes: T2 equals T4* greater than T4. This contrasts with the relative degree of interaction between the GC and tissue culture cells, which follows the relative order: T2 greater than T4 equals T4*. Trypsin pretreatment of GC markedly reduces the association of all three types (T2, T4 AND T4*) with leukocytes but does not alter the level of attachment of any of the gonococcal types with tissue culture cells.
D046508:Culture Techniques; D006069:Gonorrhea / Q000276:immunology*; D006367:HeLa Cells / Q000382:microbiology*; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D007962:Leukocytes / Q000382:microbiology*; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D014357:Trypsin
D007457:Iodine Radioisotopes; D014357:Trypsin