[Detergents and precipitation of serum lipoproteins by antisera (author's transl)].
By using gel diffusion methods, it was shown that during immunoelectrophoresis and double diffusion in two dimensions, anionic detergents (sodium oleate, sodium desoxycholate, sodium lauryl-sulfate and sodium decyl-sulfate), added to human serum at the final concentration of 0.5%, do not prevent the formation of an insoluble antigen-antibody complex (precipitation line) when specific antibodies react with serum lipoproteins: LDL, HDL and VLDL. In spite of the presence of anionic detergents, the antigen-antibody interaction in gel occurs even if the amount of lipoproteins in serum was highly reduced. On the contrary, the non ionic detergent tween 80, has a strong inhibitory effect on the specific precipitation of lipoproteins, especially in the case of HDL.
D000838:Anions; D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions / Q000187:drug effects*; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D007106:Immune Sera / Q000494:pharmacology*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008074:Lipoproteins / Q000097:blood; D009829:Oleic Acids / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D012964:Sodium; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D000838:Anions; D000936:Antigen-Antibody Complex; D003902:Detergents; D007106:Immune Sera; D008074:Lipoproteins; D009829:Oleic Acids; D011136:Polysorbates; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate; D012964:Sodium
Détergents et précipitation des lipoprotéines sériques par des immunsérums