Mediators of passive lung anaphylaxis in the rat.
Passive lung anaphylaxis (PLA) was investigated in rats sensitized by the intravenous injection of high titre reaginic antiserum prepared in rats. 2 The effect of various pharmacological antagonists on anaphylactic bronchoconstriction in vivo were examined. An antihistamine (mepyramine), a kallikrein inactivator (aprotinin) or a prostaglandin synthesis inhibitor (aspirin) did not inhibit PLA, whereas an anti-5-hydroxytryptamine agent (methysergide) and an anti-slow reacting substance-A agent (FPL 55712) significantly reduced the response. 3 Isolated perfused lungs taken from sensitized rats released, on challenge with the sensitizing antigen, histamine, 5-hydroxytryptamine, slow reacting substance of anaphylaxis (SRS-A) and prostaglandins, but no rabbit aorta contracting substance (RCS). 4 Disodium cromoglycate inhibited both anaphylactic bronchoconstriction in vivo and the anaphylactic release of mediators in vitro. Inhibition in vivo was dose-related. 5 Mediators from the intestine, the primary shock organ of anaphylaxis in the rat, did not contribute to the lung response. 6 Vagal reflex pathways were found not to be important in PLA in vivo. 7 The relationship between the mediators released following antigen challenge of passively sensitized rat lung in vitro and passive lung anaphylaxis in vivo is discussed.
D000707:Anaphylaxis / Q000188:drug therapy / Q000276:immunology*; D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D001980:Bronchi / Q000187:drug effects; D004205:Cromolyn Sodium / Q000627:therapeutic use; D005260:Female; D006632:Histamine / Q000378:metabolism / Q000494:pharmacology; D006358:Hot Temperature; D007413:Intestinal Mucosa / Q000378:metabolism; D008171:Lung Diseases / Q000276:immunology*; D011453:Prostaglandins / Q000494:pharmacology; D051381:Rats; D011935:Reagins; D013189:SRS-A / Q000494:pharmacology; D014630:Vagus Nerve / Q000187:drug effects
D011453:Prostaglandins; D011935:Reagins; D013189:SRS-A; D006632:Histamine; D007611:Aprotinin; D004205:Cromolyn Sodium; D001241:Aspirin