[Analysis of volatile aldehydes by radio-gaschromatography (author's transl)].
Soya- and oats-lipoxygenase (E.C. 1.13.1.13.) are incubated by 14C-marked linoleic acid. The volatile aldehydes arising thereby are isolated. The activity of the components separated by gaschromatography is written down by a printing indicator and the impulses/min are registered and printed out by a ratemeter. Thus the aldehydes which are produced by the enzymatic oxydation with lipoxygenase from the molecule of the linoleic acid can be determined. The composition of the mixture of aldehyde is calculated in mol-% from the measured impulses per peak. A possible origin of pathway is indicated for the main reaction products hexanal (soya-lipoxygenase) and non-trans-2-enal (oats-lipoxygenase).
D000447:Aldehydes / Q000032:analysis*; D002384:Catalysis; D002849:Chromatography, Gas; D002523:Edible Grain; D008041:Linoleic Acids; D008084:Lipoxygenase; D010084:Oxidation-Reduction; D011854:Radiochemistry; D013025:Glycine max
D000447:Aldehydes; D008041:Linoleic Acids; D008084:Lipoxygenase
Radiogaschromatographische Analyse flüchtiger Aldehyde, die bei der mit Soja- und Hafer-Lipoxygenase katalysierten Linolsäure-Oxidation entstehen