Isolation of dicarboxylic acid- and glucose-binding proteins from Pseudomonas aeruginosa.
Inducible binding proteins for C4-dicarboxylic acids (DBP) and glucose (GBP) were isolated from Pseudomonas aeruginosa by extraction of exponential-phase cells with 0.2 M MgC12 (pH 8.5) and by an osmotic shock procedure without affecting cell viability. DBP synthesis was induced by growth on aspartate, alpha-ketoglutarate, succinate, fumarate, malate, and malonate but not by growth on acetate, citrate, pyruvate, or glucose. Binding of succinate by DBP was competitively inhibited by 10-fold concentrations of fumarate and malate but not by a variety of related substances. GBP synthesis and transport of methyl alpha-glucoside by whole cells were induced by growth on glucose or pyruvate plus galactose, 2-deoxyglucose, or methyl alpha-glucoside but not by growth on gluconate, succinate, acetate, or pyruvate. The binding of radioactive glucose by GBP was significantly inhibited by 10-fold concentrations of glucose, galactose, and glucose-1-phosphate but not by the other carbohydrates tested. The binding of glucose by GBP or succinate by DBP did not result in any chemical alteration of the substrates.
D001426:Bacterial Proteins / Q000096:biosynthesis; D001667:Binding, Competitive; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D003470:Culture Media; D003998:Dicarboxylic Acids / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008757:Methylglucosides / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003470:Culture Media; D003998:Dicarboxylic Acids; D008757:Methylglucosides; D013386:Succinates; D005947:Glucose