title: Pseudomonas aeruginosa exotoxin: purification by preparative polyacrylamide gel electrophoresis and the development of a highly specific antitoxin serum.
abstract: Pseudomonas aeruginosa exotoxin has been purified to a specific activity of 12,000 to 16,000 mouse median lethal doses/mg of protein. Total recovery was about 25%, and the degree of purification was approximately 3,000-fold. Preparative polyacrylamide gel electrophoresis greatly facilitated purification. As judged by analytical disc gel electrophoresis, the purified toxin contained one major band of protein and only a negligible amount of contamination. Antiserum prepared against the purified toxin neutralized the lethal activity of crude toxin preprations and reacted by double immunodiffusion with a single component of concentrated broth cultures of P. aeruginosa isolates obtained from a clinical source.
mesh_terms: D000818:Animals; D000992:Antitoxins / Q000302:isolation & purification* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007928:Lethal Dose 50; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D014118:Toxins, Biological / Q000302:isolation & purification
chemical_list: D000992:Antitoxins; D014118:Toxins, Biological