[Synthetic medium for gamete survival and maturation and for culture of fertilized eggs].
A defined medium more similar to female genital tract fluids than to blood serum is tested on bull sperm survival, calf oocyte maturation and cleavage of Rabbit and Cow fertilized eggs: -- after incubation for 24 hrs. at 38 degrees C, 20 to 40 p. 100 of the spermatozoa remain motile in two-thirds of the samples; -- 95 p. 100 of immature calf oocytes reach metaphase II after 24 hrs. of culture in the presence of gonadotropins; -- 57 p. 100 of Rabbit morulae divide during 50 hrs. culture as compared to 40 p. 100 in HAM F/10 medium; -- 65 p. 100 of the Cow blastocysts hatch in vitro as compared to 18 p. 100 with Brinster medium.
D000596:Amino Acids; D000818:Animals; D000900:Anti-Bacterial Agents; D001205:Ascorbic Acid; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D008297:Male; D008540:Meiosis; D010063:Ovum; D011506:Proteins; D011817:Rabbits; D013081:Sperm Motility; D013094:Spermatozoa
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D003470:Culture Media; D011506:Proteins; D001205:Ascorbic Acid
Milieu synthétique pour la survie et la maturation des gamètes et pour la culture de l'aeuf fécondé