Experimental studies on mice subcutaneously challenged with heat-killed cells of Pseudomonas aeruginosa.
Mice were found to be generally refractory to sc challenge with heat-killed cells of Pseudomonas aeruginosa and did not die unless unusually high concentrations were employed. Approximately 38.6% of the animals receiving a single, sublethal dose of 1 X 10(10) dead cells developed black, crusty, necrotic skin lesions within 3 to 5 days. No major gross and histopathological changes were detected in internal organs. If the animals were sc administered sublethal doses of either live or dead cells of P aeruginosa 16 days prior to sc challenge, then the incidence of black lesions rose to 78.6 and 50% of the animals, respectively. Of several antineoplastic agents tested, only methotrexate significantly affected the 72-hr LD50 resulting in a drop to 1.8 X 10(9) afrom 3.4 X 10(10) cells. However, both methotrexate and actinomycin D decreased the incidence of the black lesions.
D000818:Animals; D003348:Cortisone / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004731:Endotoxins; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D011552:Pseudomonas Infections / Q000209:etiology* / Q000473:pathology; D011550:Pseudomonas aeruginosa; D014750:Vincristine / Q000494:pharmacology
D004731:Endotoxins; D003561:Cytarabine; D003609:Dactinomycin; D014750:Vincristine; D003348:Cortisone; D004837:Epinephrine; D008727:Methotrexate