Isolation of human T and B lymphocytes by rosette formation with 2-aminoethylisothiquronium bromide (AET) -treated sheep red blood cells with monkey red blood cells.
Purified human B and T lymphocytes were obtained by rosetting HPL with AET-SRBC or MRBC and separating the non-rosetted from the rosetted cells on Ficoll-Hypaque gradient. 92 +/- 5% of the B cells were fluorescent positive for mBlg and 95 +/- 2% of the purified T cells rosetted with AET-SRBC. 69 +/- 5% of the B cells and 61 +/- 8% of the T cells present in the unifractionated HPL were recovered in the purified fractions.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D000882:Haplorhini; D006801:Humans; D007104:Immune Adherence Reaction; D008251:Macaca; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology*
D000338:beta-Aminoethyl Isothiourea