title: Preferential inhibition of lipid synthesis by the bacteriocin pyocin S2.
abstract: The effects of pyocin S2, a bacteriocin produced by Pseudomonas aeruginosa strain M47, on several processes in susceptible bacterium have been examined. Lipid synthesis, measured in terms of [32P]phosphate, [14C]acetate or [2-3H]glycerol incorporation into lipid fractions, was halted almost completely soon after pyocin S2 addition. When cell suspensions were treated with various amounts of pyocin S2, the extent of inhibition of lipid synthesis was proportional to the ratio of killed bacteria. Protein synthesis was not essential for the inhibition. Degradation of lipid due to pyocin S2 was not detected. Pyocin S2 also affected protein and nucleic acid syntheses, but these inhibitions appeared with a delay of about 10 min after the cessation of lipid synthesis. The effect of trypsin [EC 3.4.21.4] on the viability of cells which had adsorbed pyocin S2 was also investigated: the cells went through a period when the destruction of pyocin S2 by trypsin restored the colony-forming ability of the cells (stage I). Then transition to a second state in which the cells lost viability irrespective of trypsin treatment (stage II) took place. The transition from stage I to stage II depended on the energy metabolism of the cells and followed first-order kinetics with a rate proportional to the number of killing units of adsorbed pyocin S2. The residual capacity for lipid synthesis in cells which had adsorbed pyocin S2 after trypsin treatment at various times indicated that lipid synthesis was inhibited only in the cells at stage II of pyocin S2 action.
mesh_terms: D000085:Acetates / Q000378:metabolism; D001430:Bacteriocins / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D046911:Macromolecular Substances; D010101:Oxygen Consumption / Q000187:drug effects; D010710:Phosphates / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000378:metabolism*; D011709:Pyocins / Q000494:pharmacology*; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000085:Acetates; D001430:Bacteriocins; D008055:Lipids; D046911:Macromolecular Substances; D010710:Phosphates; D011709:Pyocins; D014357:Trypsin; D005990:Glycerol