Platelet aggregation induced by antilymphocyte serum. I. Differences between washed and unwashed platelets.
Horse antilymphocyte serum (ALS) induced active release of nucleotides and aggregation of washed human platelets. It also caused lysis of platelets in the presence of plasma. The sequence of lytic processes was studied by electron microscopy. Aggregation induced by ALS was not mediated through ADP in either washed or unwashed platelets, but was inhibited by those inhibitors of secondary platelet aggregation. In unwashed platelets heparin inhibited the cytolysis and aggregation completely, while prostaglandin E1 (PGE1) did not; in contrast, PGE1 completely inhibited the aggregation and release of nucleotides from washed platelets, while heparin did not. The possible mechanisms of ALS-induced aggregation are discussed.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure; D002122:Calcium Chloride / Q000494:pharmacology; D003176:Complement C3; D004492:Edetic Acid / Q000494:pharmacology; D006041:Goats / Q000276:immunology; D006493:Heparin / Q000494:pharmacology; D006736:Horses / Q000276:immunology; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D008634:Mersalyl / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D011458:Prostaglandins E / Q000494:pharmacology; D013743:Tetanus Antitoxin / Q000494:pharmacology
D000961:Antilymphocyte Serum; D003176:Complement C3; D011458:Prostaglandins E; D013743:Tetanus Antitoxin; D008634:Mersalyl; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006493:Heparin; D004492:Edetic Acid; D008274:Magnesium; D002122:Calcium Chloride; D001241:Aspirin