[The effect of lead on cells cultivated in vitro. I. Acute effects (author's transl)].
Static suspension cultures of L-A cells, a subline of L929 mouse fibroblasts, were exposed to inorganic lead salts. Lead caused a growth inhibition which was independent of the anion used and which could be prevented by the addition of Ca-EDTA. The LD50 was approximately 1 mM when exposing the cells for 2 days and 2 X 10(-4) M when treating them for 7 days. The minimal growth inhibiting dose was in the range of 2 to 4 X 10(-5) M. Concomitant with the dose-dependent inhibition of proliferation the fraction of cells synthesizing DNA and the mitotic rate were lowered, suggesting a block in the G1 phase of the cell cycle. Energy metabolism appeared affected as evidenced by a rise in lactate production. Lactate dehydrogenase release was in the control range. Lead induced an increase in cell size and, at higher concentrations, cellular vacuolations.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004492:Edetic Acid / Q000494:pharmacology; D007739:L Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D007773:Lactates / Q000096:biosynthesis; D007854:Lead / Q000494:pharmacology*; D007928:Lethal Dose 50; D051379:Mice; D008940:Mitotic Index
D007773:Lactates; D007854:Lead; D004247:DNA; D004492:Edetic Acid; D007770:L-Lactate Dehydrogenase
Einfluss von Blei auf in vitro gezüchtete Zeller I. Akute Wirkungen