Immunofluorescent localization of bovine acrosin.
Acrosin, a trypsin-like proteinase, was localized in the acrosome of methanol-fixed bovine spermatoza by the indirect immunofluorescent technique. Anti-acrosin immunoglobulin was obtained from a rabbit immunized with highly purified bovine acrosin. The results of agarose-gel immunnodiffusion analyses indicated that although the antibody was specific for the acrosomal enzyme, it cross-related with ovine acrosin.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D010450:Endopeptidases / Q000276:immunology* / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique / Q000379:methods; D005779:Immunodiffusion; D008297:Male; D011817:Rabbits / Q000276:immunology; D013094:Spermatozoa / Q000201:enzymology* / Q000276:immunology
D010450:Endopeptidases