[Influence of autolysis on the immunohistology of tissue-bound immunglobulins in the kidney (author's transl)].
The influence of autolysis on the immunhistological detectability and the immunological properties of tissue-bound basement membrane antibodies were investigated in the rat kidney. The immunhistological demonstration of antibodies was not affected up to 3 days post mortem. The disturbing autofluorescence of the autolytic tissue can be avoided by using peroxydase labeled antibodies. Basement-membrane antibodies that were eluted from the kidneys after an autolysis period of 2 days showed a homogenous linear deposition in kidney basement membrane after reinjection into normal rats. Their antibody properties were not affected.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D001329:Autolysis; D001485:Basement Membrane / Q000276:immunology; D005453:Fluorescence; D006652:Histological Techniques; D007136:Immunoglobulins / Q000032:analysis*; D007668:Kidney / Q000276:immunology*; D010544:Peroxidases; D051381:Rats; D013997:Time Factors
D000906:Antibodies; D007136:Immunoglobulins; D010544:Peroxidases
Der Einfluss der Autolyse auf die immunhistologische Darstellbarkeit gewebegebundener Immunglobuline in der Niere