Effects of selected water toxicants on the in vitro activity of fish carbonic anhydrase.
Carbonic anhydrase (CA, carbonate hydro-lyase EC 4.2.1.1) from lysed red blood cells of the channel catfish (Ictalurus punctatus) was studied to determine physico-chemical kinetic properties and relative sensitivity to 56 chemicals of significance in water quality research. In general, enzyme activity was strongly reduced by heavy metal inorganic cations; less strongly by organometallic cations, some anions, and certain pesticides; and weakly inhibited by light metal cations and organometallic and organic compounds. Dose--response curves were qualitatively similar in most cases.
D000818:Animals; D002256:Carbonic Anhydrases / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology*; D005399:Fishes / Q000378:metabolism*; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014873:Water Pollutants / Q000506:poisoning*; D014874:Water Pollutants, Chemical / Q000506:poisoning*
D008670:Metals; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D002256:Carbonic Anhydrases