title: [Purification of purine nucleoside phosphorylase activity on deoxyinosine (author's transl)].
abstract: Purified preparations of pigeon liver PNPase (E.C. 2.4.2.1) have been obtained by acid preparation of liver homogenates at pH = 5,followed by a fractionation with ammonium sulphate (25-50% saturation) and by a chromatographic adsorption on DEAE-cellulose. The preparation obtained shows a PNPase specific activity 325 times greater than that of the original homogenates. Kinetic studies carried out with homogenates and purified preparations of pigeon liver PNPase seem to suggest that inosine and deoxynosine react on the same catalytic site of the enzyme molecule.
mesh_terms: D000818:Animals; D010856:Columbidae; D003853:Deoxyribonucleosides / Q000378:metabolism; D007288:Inosine / Q000031:analogs & derivatives* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010430:Pentosyltransferases / Q000302:isolation & purification*; D011683:Purine-Nucleoside Phosphorylase / Q000302:isolation & purification*
chemical_list: D003853:Deoxyribonucleosides; D007288:Inosine; D010430:Pentosyltransferases; D011683:Purine-Nucleoside Phosphorylase; C012271:deoxyinosine
vernacular_title: Purificación de la purinnucleósido fosforilasa. Actividad frente a desoxinosina