[Study of humoral immunity in human biharziasis. Value of E.L.I.E.D.A. (enzyme-linked immunoelectrodiffusion assay)].
With a view to sensitize the immuno-electro-diffusion test (I.E.D.) and determine the immunoglobulins involved in the antigen-antibody reaction, the authors have improved the I.E.D. test by coupling it with an enzymatic technique. The precipitated immune-complexes are treated with antibodies conjugated with an enzyme specific for each class of immuno-globulins. This association realizes the E.L.I.E.D.A. (enzyme-linked immuno-electro-diffusion-assay). The sensitivity and specificity of the method are excellent, and should allow to follow the qualitative evolution of the bilharzian antibody in natural and experimental affections.
D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D005069:Evaluation Studies as Topic; D006801:Humans; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D010544:Peroxidases; D012550:Schistosoma mansoni / Q000276:immunology; D012552:Schistosomiasis / Q000276:immunology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D010544:Peroxidases
Etude de l'immunité humorale dans la bilharziose humaine. Intérêt de l'E.L.I.E.D.A. (enzyme-linked-immuno-electro-diffusion-assay