Isolation and immunochemical characterization of the group-specific antigen of Streptococcus mutants 6715.
The group d antigen of Streptococcus mutans 6515 was isolated from a buffer (pH 7.3)-boiled extract of whole cells and analyzed immunochemically. Rabbits immunized in three different fashions with whole S. mutans 6715 each responded to the same antigenic cell surface component. This presumptive major antigen was found in culture supernatant, sonically treated supernatant, acid and buffer extracts of whole cells, and trichloroacetic acid extract of cell membranes. A crude preparation of this antigen could completely inhibit antibody-mediated cell (S. mutans 6715) agglutination in a spectrophotometric analysis. The antigen was purified from buffer-boiled extracts by gel filtration on columns of Sepharose 4B. The antigen did not migrate to the anode on electrophoresis nor did it contain appreciable quantities of phosphorus, glycerol, or ribitol. This suggested that the d antigenicity did not reside in a teichoic acid. The d antigen contained galactose and glucose as the sole saccharides, in a ratio of 5.9:1.0. Protein (9.5%) appeared to be a portion of the antigen, although Pronase-digested antigen retained the same electrophoretic mobility and could precipitate virtually all (98.6%) purified antibody directed to the intact antigen. The data obtained from hapten innvolved. Glucose also contributed to the immunodominant region. Antibody directed to the d antigen may be of importance in the inhibition of adherence phenomena manifested by S. mutans organisms of the d group.
D000143:Acids; D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002021:Buffers; D002850:Chromatography, Gel; D006601:Hexoses / Q000032:analysis; D007106:Immune Sera; D007120:Immunochemistry; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D011134:Polysaccharides / Q000494:pharmacology; D011233:Precipitin Tests; D011402:Pronase; D011817:Rabbits / Q000276:immunology; D013053:Spectrophotometry; D013291:Streptococcus / Q000276:immunology*; D014238:Trichloroacetic Acid
D000143:Acids; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D002021:Buffers; D006601:Hexoses; D007106:Immune Sera; D011134:Polysaccharides; D014238:Trichloroacetic Acid; D011402:Pronase