The genetic control of T cell-mediated immunity against the DBA/2 mastocytoma P-815. II. Low responsiveness in T cell-mediated cytotoxicity accompanied by the inability to produce antibodies in a secondary response.
Low responsiveness of DBA/1 mice in T cell-mediated cytotoxicity against the DBA/2 mastocytoma P-815 (H-2d) is genetically controlled and inherited as a recessive trait. For an analysis of the humoral anti-H-2d response of DBA/1 mice after immunization with P-815 mastocytoma cells both complement-dependent lysis (CDL) and the cytotoxicity inhibition assay (CIA) were used to demonstrate antibodies. Both assays show that DBA/1 mice are unable to produce anti-H-2d antibodies in a secondary response. Even high doses of immunizing cells give only a weak response. The response is fully recovered upon allogeneic interaction. A transient primary anti-H-2d activity can be found in the CIA but not by CDL. The data show that the genetically controlled determinant-specific defect in T cell-mediated cytotoxicity among DBA/1 mice has its correlate in a blockade of memory formation or expression in the humoral anti-H-2d response.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis; D000917:Antibody Formation; D003520:Cyclophosphamide / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005808:Genes, Recessive; D006649:Histocompatibility Antigens; D007111:Immunity, Cellular / Q000187:drug effects; D007156:Immunologic Memory; D007518:Isoantibodies / Q000096:biosynthesis; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000912:Antibodies, Neoplasm; D006649:Histocompatibility Antigens; D007518:Isoantibodies; D003520:Cyclophosphamide