Bioautographic characterization of antimicrobial substances after electrophoresis in agar gel.
Bioautography following electrophoresis on agar gel, a technique for the characterization of antibiotics, is described in detail and its possible uses and advantages discussed. Eleven antibiotics of the beta-lactamic group were characterized. Penicillin G and Penicillin V showed two components with anti-microbial activity. Attempts were made to identify the components by an adaptation of the technique and the results are discussed.
D000667:Ampicillin; D000900:Anti-Bacterial Agents; D001681:Biological Assay; D002509:Cephaloridine; D002511:Cephalosporins; D002512:Cephalothin; D004587:Electrophoresis, Agar Gel; D008712:Methicillin; D008851:Micropore Filters; D010400:Penicillin G; D010404:Penicillin V / Q000031:analogs & derivatives; D011343:Procaine
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D011343:Procaine; D000667:Ampicillin; D002509:Cephaloridine; D010400:Penicillin G; D008712:Methicillin; D002512:Cephalothin; D010404:Penicillin V