title: Degradation of thyrotropin releasing hormone and a related compound by rat liver and kidney homogenate.
abstract: The inactivation of the hypothalamic hormone by rat liver and kidney homogenates was studied, using specific radioimmunoassays for the measurement of thyrotropin releasing hormone, pGlu-His-Pro-NH2 (TRH), and for an analogous peptide, pGlu-His-Pro-OH(TRH-OH), which has been proposed as a major metabolite of TRH [NAIR et al., 1971]. The inactivation of TRH and the free acid was found to be rapid. Heat lability and saturation kinetics suggest the involvement of enzymatic processes. In liver homogenate, TRH-OH production from TRH was observed. The accumulation of TRH-OH was substantial in experiments employing near-saturation concentrations of TRH. The liver and kidney are ascribed as major sites for breakdown of TRH in vivo.
mesh_terms: D000818:Animals; D003429:Cross Reactions; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*
chemical_list: D013973:Thyrotropin-Releasing Hormone