A radioimmunoassay for rat serum parathyroid hormone using N-terminal of PTH.
Partially purified bovine parathyroid hormone (PTH) produced two kinds of antisera, GP-100 reacting with 125I-labeled bovine PTH (b-PTH 1-84) alone and GP-M reacting with 125I-labeled synthetic N-terminal portion of bovine PTH (b-PTH 1-34) as well as b-PTH 1-84.  Rat serum PTH reacted with the latter but not in the former, suggesting a similarity to b-PTH 1-34.  Rat serum PTH became undetectable after parathyroidectomy, and increased after Na2-EDTA injection and following renal injury with sodium sulfacetylthiazole.
D000818:Animals; D002417:Cattle; D004492:Edetic Acid / Q000378:metabolism; D006168:Guinea Pigs / Q000276:immunology; D007106:Immune Sera; D007457:Iodine Radioisotopes; D008297:Male; D010280:Parathyroid Glands / Q000601:surgery; D010281:Parathyroid Hormone / Q000097:blood* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D012964:Sodium / Q000378:metabolism
D007106:Immune Sera; D007457:Iodine Radioisotopes; D010281:Parathyroid Hormone; D004492:Edetic Acid; D012964:Sodium