Evolution of the transcription complex during sporulation of Bacillus subtilis.
Ribonucleic acid polymerase activity in partially purified extract of cells of Bacillus subtilis harvested at different times (t-1, to, t1, and t2) was studied by zone centrifugation. During the course of sporulation, vegetative sigma-factor activity decreased and the transcription complex lost some of its affinity for active sigma factor. The complex underwent a two-stage change in sedimentation value, from 14.5S in vegetative growth phase to a 13S species very early in sporulation to a 16S species at later times. Two SpoO mutants have been studied by zone centrifugation. One strain, a rifampin-resistant (RfmR) mutant, failed to show any modification of the transcription complex, whereas the other, a Rfms strain, underwent a partial evolution of the transcription complex after to.
D001412:Bacillus subtilis / Q000201:enzymology / Q000254:growth & development*; D002474:Cell-Free System; D002501:Centrifugation, Zonal; D003090:Coliphages; D004279:DNA, Viral / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects; D009154:Mutation; D011119:Polynucleotides / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology; D013171:Spores, Bacterial / Q000201:enzymology / Q000254:growth & development*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004279:DNA, Viral; D011119:Polynucleotides; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin