[Hydrolysis of diphosphatidylglycerol in situ in E. coli by activation of specific phospholipase D].
E coli cells are harvested at early stationary phase and, first, incubated in phosphate buffer without energetic metabolites, conditions which invole the formation of an excess of diphosphatidylglycerol. If, in a second stage, they are cultivated again in normal medium, the diphosphatidylglycerol is metabolized very fast. It is mainly hydrolyzed, by activation of a specific phospholipase D, with formation of phosphatidylglycerol and phosphatidic acid. The latter is immediatly metabolized in bacteria in phosphatidylglycerol and phosphatidylethanolamine, both about equal in amount.
D003470:Culture Media; D004734:Energy Metabolism; D004926:Escherichia coli / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*
D003470:Culture Media; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010743:Phospholipids; D010740:Phospholipases
Hydrolyse du diphosphatidylglycérol in situ chez E. coli par activation d'une phospholipase D spécifique