title: Effect of heat shock on the synthesis of low molecular weight RNAs in drosophilia: accumulation of a novel form of 5S RNA.
abstract: The synthesis and stability of low molecular weight RNAs following heat shock in Drosophilia melanogaster cell cultures have been examined. When cultures are raised from 25 degrees C to 37 degrees C, the synthesis of tRNA and at least two other low molecular weight RNAs continues at the 25 degree C rate. 5.8S ribosomal RNA and most of the low molecular weight nuclear RNAs are not synthesized. The synthesis of 5S ribosomal RNA is greatly reduced. A large amount of an RNA of about 135 nucleotides in length accumulates at 37 degrees C. Nucleotide sequence analysis reveals that this RNA is a novel form of 5S RNA with approximately 15 additional nucleotides at its 3' end.
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D004331:Drosophila melanogaster / Q000378:metabolism*; D006358:Hot Temperature; D008970:Molecular Weight; D009843:Oligoribonucleotides / Q000032:analysis; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis
chemical_list: D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D012313:RNA; D012343:RNA, Transfer