Preparation of specific antisera to Drosophila acid phosphatase without rigorous protein purification.
Extracts from an acid phosphatase CRM- null mutant of Drosophila melanogaster were used to eliminate contaminating antibodies in a nonspecific preparation of anti-acid phosphatase serum. This method of producing specific antisera makes unnecessary the rigorous purification of an antigen prior to immunization attempts in those cases where CRM- null mutants of the antigen are available. Antisera so prepared could be used for a wide variety of purposes.
D000135:Acid Phosphatase / Q000276:immunology*; D000483:Alleles; D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology; D007106:Immune Sera / Q000302:isolation & purification*; D005779:Immunodiffusion; D008722:Methods; D011817:Rabbits / Q000276:immunology
D007106:Immune Sera; D000135:Acid Phosphatase