Prostaglandin E2 biosynthesis: changes in rabbit aorta and skin during experimental atherogenesis.
The transformation of [1-14C]arachidonic acid into [14C]prostaglandin E2(PGE2) by rabbit aorta and skin was demonstrated by cell-free preparations, and the PGE2 synthetase activity was located mainly in the microsomal fraction (105,000 g pellet) of both tissues. Rabbits fed an atherogenic diet (Purina rabbit chow plus 1% cholesterol and 2% cottonseed oil) developed atheroma in the aortas and skin lesions resembling xanthoma in 6 to 7 months. At  the end of this period, increases in the conversion of [1-14C]-arachidonic acid into [14C]PGE2 were observed in microsomal preparations of the intima-media of the aortas (2.5-fold of control) and normal-appearing skin (3.0-fold of control) of the experimental animal. Microsomal preparations of skin lesions particularly had greater ability to form PGE2 (7-fold of control).
D000818:Animals; D001011:Aorta / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D001095:Arachidonic Acids / Q000378:metabolism; D001161:Arteriosclerosis / Q000378:metabolism*; D002791:Cholesterol, Dietary; D004036:Diet, Atherogenic; D008297:Male; D008861:Microsomes / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011817:Rabbits; D012867:Skin / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors
D001095:Arachidonic Acids; D002791:Cholesterol, Dietary; D011451:Prostaglandin-Endoperoxide Synthases