[Detection of alpha 1-antitrypsin in unfertilized ova of albino rats].
500--700 rat oocytes were solubilized in 0.3--0.6 ml of 1% sodium dodecyl sulphate (SDS) --0.06 tris-HCl, pH 6.7. The excess of SDS was removed in the form of unsoluble Ba2 (SDS) and the lysate was placed on the surface of 1% agarose microgel, performed in a glass capillar with the inner diameter 600 and the length of gel 7--8 nm. The microvolume (1.3 mcl) of monospecific antiserum against alpha1-human antitrypsin was placed on the opposite surface of the agarose gel. After 48 hours, the amount of microimmunoprecipitate was determined by staining the gel with Amido Black 10 B, followed by quantitative scanning using a double wave microspectrophotometer. The mean value of alpha1-antitrypsin was about 2.3+/-1.4 pcg per ovum. It is supposed that this antienzyme may terminate the "zone" and "cortical" reactions during the fertilization in mammals.
D000818:Animals; D003720:Densitometry; D005260:Female; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion / Q000379:methods; D009865:Oocytes / Q000201:enzymology*; D010063:Ovum / Q000201:enzymology*; D011817:Rabbits; D051381:Rats; D013053:Spectrophotometry / Q000379:methods; D000515:alpha 1-Antitrypsin / Q000032:analysis*
D007106:Immune Sera; D000515:alpha 1-Antitrypsin
Obnaruzhenie alpha 1-antitrypsin v neoplodotvorennykh iaĭtsakh belykh krys.