Improved estimation of urinary myoglobulin by counterimmunoelectrophoresis, as compared with the double immunodiffusion technique.
We describe a simple, sensitive, and relatively inexpensive counterimmunoelectrophoretic technique for detection and estimation of myoglobin. The test can be completed within 90 min, compared with 12-48 h required by double immunodiffusion. With the counterimmunoelectrophoretic technique we could detect myoglobin in concentrations of 2-3 mg/liter, while the detection limit by double immunodiffusion was about 9 mg/liter.
D005069:Evaluation Studies as Topic; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis / Q000295:instrumentation; D009212:Myoglobinuria / Q000652:urine; D013997:Time Factors