Photosynthetic activities of membrane preparations of the blue-green alga Phormidium luridum.
Techniques have been developed for the isolation of whole spheroplaste and membrane preparations from the blue-green alga Phormidium luridum which are active in photosystem-II-sensitized and photosystem-I-sensitized electron-transport reactions and in photophosphorylation. Attention is called to the necessity of supplying magnesium and phosphate throughout the preparative and assay procedures for obtaining active membranes. Selective washing of a proper membrane preparation is shown to remove a new protein factor, termed protein factor 1, which restores the ability of the preparation ot catalyze photosystem-I-sensitized reactions.
D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D000458:Cyanobacteria / Q000378:metabolism*; D004579:Electron Transport; D008274:Magnesium / Q000494:pharmacology; D008353:Mannitol / Q000494:pharmacology; D008854:Microscopy, Electron; D010785:Photophosphorylation; D010788:Photosynthesis / Q000187:drug effects; D011189:Potassium Chloride / Q000494:pharmacology; D013104:Spheroplasts / Q000187:drug effects / Q000378:metabolism
D008353:Mannitol; D011189:Potassium Chloride; D008274:Magnesium; D002118:Calcium