The relation between the T cells responsible for cell-mediated cytotoxic killing of mastocytoma cells and the helper-cell effect.
The relationship between T cells involved in cell-mediated immunity and antibody response of C57Bl/6J mice towards DBA/2 mastocytoma cells was investigated. Spleen cells primed with viable mastocytoma cells demonstrated marked cell-mediated cytotoxicity (CMC) in vitro, but antibody response of these cells to a hapten (TNP) conjugated to the allogeneic tumour cells in vitro was suppressed as compared with that of normal spleen cells. In contrast, spleen cells primed with frozen-thawed mastocytoma cells showed no CMC, but antibody response to the hapten in vitro of these cells was enhanced. C57Bl/6J mice primed with frozen-thawed mastocytoma cells produced more cytotoxic antibody than non-primed mice when immunized with viable mastocytoma cells. These results indicate that T cells involved in cell-mediated immunity and those involved in the antibody response to allogeneic mastocytoma cells are distinct.?222
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D003601:Cytotoxicity Tests, Immunologic; D004247:DNA / Q000096:biosynthesis; D005615:Freezing; D006241:Haptens; D007111:Immunity, Cellular; D007114:Immunization; D007518:Isoantibodies; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA / Q000276:immunology; D009374:Neoplasms, Experimental / Q000276:immunology; D009578:Nitrobenzenes / Q000276:immunology; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
D006241:Haptens; D007518:Isoantibodies; D009578:Nitrobenzenes; D004247:DNA