Activation of alanine aminotransferase in serum by pyridoxal phosphate.
Alanine aminotransferase activity in serum increases significantly when serum is incubated with pyridoxal phosphate. The increase depends on the L-alanine concentration in the final assay mixture, being greatest at 800 mmol/liter. Preincubation of 22 normal sera, in a 10:1 ratio with an 8.09 mmol/liter pyridoxal phosphate solution, resulted in an increase in the alanine aminotransferase activity from 10.5 +/- 4.9 U/liter (mean +/- SD) to 28.4 +/- 5.3 U/liter, an increase of 170%. The absolute amount of apoalanine aminotransferase is relatively constant over a wide range of enzyme activities.
D000409:Alanine / Q000494:pharmacology; D000410:Alanine Transaminase / Q000097:blood*; D004789:Enzyme Activation; D006801:Humans; D007656:Ketoglutaric Acids / Q000494:pharmacology; D011732:Pyridoxal Phosphate / Q000494:pharmacology*
D007656:Ketoglutaric Acids; D011732:Pyridoxal Phosphate; D000410:Alanine Transaminase; D000409:Alanine