[Culture of the bovine graafian follicle in a continuous liquid and gas flow system].
The culture of 3-5 mm calf follicles in a new glass perfusion cell is compared to the organotypic grid culture using the same synthetic medium and a gas mixture of 55% O2 + 40% N2 and 5% CO2 Amino acid consumption is 3 to 39 times higher in the perfusion cell culture and the mitotic rate is also higher. Moreover, the cytological aspect of granulosa and cumulus cells looks similar to that of non-atresic ovarian follicles. Meiosis resumes up to metaphase 1 or metaphase 2. No pyknotic cells are observed.
D000388:Air; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003470:Culture Media; D005260:Female; D006062:Gonadotropins; D006107:Granulosa Cells / Q000166:cytology; D008540:Meiosis; D008938:Mitosis; D009924:Organ Culture Techniques / Q000379:methods*; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology; D010477:Perfusion
D000596:Amino Acids; D003470:Culture Media; D006062:Gonadotropins
Culture du follicule de De Graaf de bovin dans un système à courant liquide et gazeux continu