Uptake of bacterial DNA by Chlamydomonas reinhardi.
Escherichia coli [3H]DNA supplied to vegetative cultures of wild-type (mt+) and CW15 (mt+;mutant lacking the cell wall) Chlamydomonas reinhardi could bind to the cell wall of the wild-type and to the cell membrane of CW15 mutant cells. The extent of this binding decreased with time and was to a large degree (over 90%) DNA-ase-sensitive. Nevertheless, about 0.01% of the bacterial DNA remained irreversibly associated with the cells when they reached stationary phase. The irreversible binding of the donor bacterial DNA to Chlamydomonas cells could be increased by treatment of the cultures with polycations such as DEAE-dextran, poly-L-lysine and poly-L-ornithine. Although the CW15 cells rapidly degraded bacterial DNA in the culture medium wild-type cells showed only a small effect on the molecular weight of the donor DNA. The acid-insoluble radioactivity irreversibly bound to WT (+) cells consisted mainly of oligonucleotides with a small proportion present as less depolymerized donor DNA. No radioactivity, however, was found to be associated with the recipient high molecular weight Chlamydomonas DNA. No labeled donor DNA could be recognized in the cells given bacterial [3H]DNA in early stationary phase. Instead, radioactivity found in Chlamydomonas DNA corresponded to reutilization of [3H]thymine derivatives released as a result of [3H]DNA degradation. No evidence for the integration of detectable amounts of donor DNA sequences into the host cell DNA was obtained.
D001412:Bacillus subtilis; D001665:Binding Sites; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002696:Chlamydomonas / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D003637:DEAE-Dextran / Q000494:pharmacology; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli; D007700:Kinetics; D008970:Molecular Weight; D009952:Ornithine / Q000494:pharmacology; D011107:Polylysine / Q000494:pharmacology; D014316:Tritium
D004269:DNA, Bacterial; D014316:Tritium; D011107:Polylysine; D003637:DEAE-Dextran; D009952:Ornithine