title: Glycogen synthase can use glucose as an acceptor.
abstract: Glycogen synthase purified to homogeneity from rabbit skeletal muscle is essentially free of carbohydrate and shows no activity in the absence of added acceptor. It can use glucose as a substrate converting it to a glucose disaccharide in the presence of UDP-glucose as cosubstrate. The reaction is dependent on time, and on UDP-glucose, glucose, and enzyme concentrations. The product of the single step reaction co-chromatographs in two solvent systems with maltose. The glucose disaccharide produced in the reaction with UDP-[14-C]glucose and nonradioactive glucose as well as with nonradioactive UDP-glucoes and [14-C]glucose is labeled asymmetrically. The linkage is characterized as alpha-1,4 and therefore the disaccharide is identified as maltose.
mesh_terms: D000818:Animals; D005947:Glucose / Q000378:metabolism*; D005959:Glucosidases; D006006:Glycogen Synthase / Q000378:metabolism*; D007700:Kinetics; D008968:Molecular Conformation; D009132:Muscles / Q000201:enzymology; D009844:Oligosaccharides / Q000096:biosynthesis; D011817:Rabbits; D014539:Uridine Diphosphate Sugars
chemical_list: D009844:Oligosaccharides; D014539:Uridine Diphosphate Sugars; D006006:Glycogen Synthase; D005959:Glucosidases; D005947:Glucose