A comparative study of preparations of ovine lutenizing hormone by bioassay, immunodoublediffusion, radioimmunoassay, radioreceptor assay and polyacrylamide gel electrophoresis.
Three preparations of ovine LH were compared for biological potency and by several in vitro parameters. All were found to be heterogenous by immunodoublediffusion and by electrophoresis in polyacrylamide gels. They all also showed similarities and/or differences with respect to their characteristics in immunodoublediffusion, radioimmunoassay, radioreceptor assay, gel electrophoresis and in dye-binding capacity, but in ways that preclude establishing a meaningful correlation between biopotency and the in vitro parameters or even among the in vitro parameters themselves. The implications of these findings for the use of these in vitro parameters for screening and assessing biological potencies of LH preparations and for inferring chemical and/or structural similarities between LH preparations are discussed. Aspects of polymorphism of LH, observed by electrophoresis on polyacrylamide gels, are also discussed.
D000818:Animals; D001681:Biological Assay; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007986:Luteinizing Hormone / Q000032:analysis*; D011863:Radioimmunoassay; D011869:Radioligand Assay; D012756:Sheep
D007986:Luteinizing Hormone