title: Reconstitution of Rh (D) antigen activity from human erythrocyte membranes solubilized by deoxycholate.
abstract: Proteins were selectively solubilized from human erythrocyte membranes with deoxycholate. After ultracentrifugation and preliminary fractionation procedures, the detergent was removed from the soluble membranes by Bio-Beads SM-2 and dialysis against 5 millimolar magnesium chloride. Reaggregation took place with the apparent formation of vesicles which showed serologically specific Rh antigen activity.
mesh_terms: D002458:Cell Fractionation; D002462:Cell Membrane / Q000276:immunology; D003840:Deoxycholic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D008970:Molecular Weight; D012204:Rh-Hr Blood-Group System / Q000302:isolation & purification*; D012995:Solubility; D014462:Ultrafiltration
chemical_list: D012204:Rh-Hr Blood-Group System; D003840:Deoxycholic Acid