Synthesis, storage and drug-induced release of atp-8-3h in the perfused bovine adrenal gland.
The perfused bovine adrenal gland was able to take up and phosphorylate adenosine-8-3 H. Stimulation of the gland with carbachol resulted in a simultanious release of catecholamines, adenosine-8-3 H and labelled adenine nucleotides including a small quantity of ATP-3 H. A direct demonstration of the presence of ATP-3 H in isolated chromaffin granules was obrained by modified sucrose density gradient centrifugation. Glands were also perfused with a Ca++-Mg++-free-EDTA medium to inhibit adenosine triphosphatases. Using this medium, significant amounts of ATP-3 H and endogenous ATP were found in the effluent after drug stimulation by either Ba++ of aminophylline. Under Ca++-Mg++-free-EDTA conditions both Ba++ and aminophylline, but not carbachol, stimulated the release of catecholamines, ATP, ATP-3 H with no concomitant increase in total radioactivity. Using normal medium, Ba++ caused the simultaneous release of catecholamines and radioactivity while aminophylline only induced the release of catecholamines. Specific activity measurements of drug-released ATP indicated that the newly synthesized ATP-3 H was being released preferentially during spontaneous activity while the endogenous ATP was preferentially released after either Ba++ or aminophylline-induced secretion.
D000241:Adenosine / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis / Q000378:metabolism*; D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism*; D000628:Aminophylline / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002395:Catecholamines / Q000378:metabolism; D002417:Cattle; D003864:Depression, Chemical; D004492:Edetic Acid; D013268:Stimulation, Chemical; D013997:Time Factors
D002395:Catecholamines; D001464:Barium; D000628:Aminophylline; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002217:Carbachol; D004492:Edetic Acid; D000241:Adenosine