[A one-step chromatographic preparation of alpha-L-fucosidase from the rat epididymis].
An alpha-L-fucosidase has been purified from Rat epididymis homogenate in two steps: 1, ammonium sulfate precipitation (64 p. 100 saturation) 2, "DEAE-Sephadex A 50" chromatography. The purified glycosidase was obtained with good yield and with purification degree consistent with oligosaccharide on glycopeptide structural studies.
D000818:Animals; D002852:Chromatography, Ion Exchange; D004186:Disaccharidases / Q000302:isolation & purification*; D004822:Epididymis / Q000201:enzymology*; D008297:Male; D051381:Rats; D005644:alpha-L-Fucosidase / Q000302:isolation & purification*
D004186:Disaccharidases; D005644:alpha-L-Fucosidase
Préparation en une étape chromatographique de la alpha-L fucosidase d'épididyme de de Rat