title: [Purification of deamidase AG (asparaginase-glutaminase) from Pseudomonas fluorescens AG and some physicochemical properties of the enzyme].
abstract: Deamidase AG (asparaginase-glutaminase) was obtained from Pseudomonas fluorescens in a crystalline apparently homogenous state. Molecular weight of the enzyme, determined by acrylamide gel electrophoresis, was equal to 128 000 daltons; by ultracentrifugation (56100 rev/min, 65 min, 20.5 degrees C) coefficient of sedimentation was shown to be 7.36 S. Optimal pH for asparaginase activity of the enzyme was at pH 8.0-9.0, for glutaminase activity--at pH 5.5-7.5.
mesh_terms: D000641:Ammonia / Q000096:biosynthesis; D001215:Asparaginase / Q000302:isolation & purification* / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002848:Chromatography, DEAE-Cellulose; D003460:Crystallization; D003470:Culture Media; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005972:Glutaminase / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D014461:Ultracentrifugation
chemical_list: D003470:Culture Media; D000641:Ammonia; D001215:Asparaginase; D005972:Glutaminase
vernacular_title: Ochistka dezamidazy AG (asparaginazy-gliutaminazy) iz Pseudomonas fluorescens AG i nekotorye fiziko-khimicheskie svoĭstva fermenta