title: Nucleotide sequences of the T1 and pancreatic ribonuclease digestion products from some large fragments of the 23S RNA of Escherichia coli.
abstract: When the 23S RNA from E. Coli was pretreated for 1 h at 60 degrees in the presence of Mg++ and K+ and then subjected to T1 ribonuclease attack, resistant fragments were recovered from 3 regions of the molecule: region A (containing 470-500 nucleotides) located at the 5' end of 23S RNA, region B (containing 520-550 nucleotides) located at the 3' end and region C (containing 110-120 nucleotides) lying between region A and region B. The nucleotide sequences of the T1 and pancreatic ribonuclease digestion products from these 3 regions have been studied and in most cases determined. In the course of these studies, a certain number of abnormal nucleotides, which are not methylated, have been encountered. A low level of sequence heterogeneity was detected.
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis*; D005796:Genes; D006868:Hydrolysis; D008274:Magnesium; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D010179:Pancreas / Q000201:enzymology; D011188:Potassium; D011485:Protein Binding; D012329:RNA, Bacterial / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis*; D006163:Ribonuclease T1 / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D012265:Ribonucleotides / Q000032:analysis; D012270:Ribosomes; D013329:Structure-Activity Relationship
chemical_list: D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012265:Ribonucleotides; D012260:Ribonucleases; D006163:Ribonuclease T1; D008274:Magnesium; D011188:Potassium