[The chromophore of blue dextran: a powerful ligand for the purification of interferon by affinity chromatography].
The chromophore of blue dextran can be used as ligand for affinity chromatography of interferon. One passage of a crude interferon preparation on a blue-dextran-sepharose column eliminates over 90% of the contaminating proteins. Desorption with an aqueous buffer of high molarity yields interferon preparations with specific activities of up to 4.5 X 10(6) units per milligram of protein and the recovery is 100%.
D000818:Animals; D002846:Chromatography, Affinity; D003911:Dextrans; D007372:Interferons / Q000302:isolation & purification*; D008024:Ligands; D008722:Methods; D051379:Mice
D003911:Dextrans; D008024:Ligands; D007372:Interferons
Le chromophore du bleu dextran: un ligand puissant pour la purification de l'interféron par chromatographie d'affinité