Chromatographic methods for the analysis of hexachlorobenzene and possible metabolites in monkey fecal samples.
The separation and identification of hexachlorobenzene (HCB), pentachlorobenzene (QCB), and pentachlorophenol (PCP) by thin layer, high-pressure liquid, and gas chromatography are reported. Satisfactory results were obtained with reverse phase thin layer and high-pressure liquid chromatography. The 2 gas chromatographic columns tested gave adequate separation of HCB and QCB. A PCP methylation process using dimethyl sulfate was attempted. Procedures are presented for extraction, cleanup, separation, and identification of HCB and possible metabolites from feces of a rhesus monkey treated with 14C-HCB. Recovery of radioactivity in excess of 96% was attained in extracts from feces by these methods. Preliminary evidence suggests the formation of PCP and possibly other polar metabolites from HCB in the rhesus monkey.
D000818:Animals; D002722:Chlorobenzenes / Q000032:analysis*; D002845:Chromatography / Q000379:methods*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005243:Feces / Q000032:analysis*; D000882:Haplorhini; D006581:Hexachlorobenzene / Q000032:analysis* / Q000378:metabolism
D002722:Chlorobenzenes; D006581:Hexachlorobenzene