Relative quantitative assay of the biological activity of interferon messenger ribonucleic acid.
RNA extracted from cells previously stimulated to synthesize the antiviral protein, interferon, causes species-specific interferon synthesis when added to heterospecific cell cultures. Our results confirm the report of De Maeyer-Geugnard, De Maeyer & Montagnier (1972). We have used this observation to obtain a relative quantitative assay for the interferon messenger RNA activity. At appropriate RNA concentrations, the yield of interferon is proportional to the concentration of RNA adsorbed to recipient cell cultures.
D000327:Adsorption; D000818:Animals; D002460:Cell Line; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003637:DEAE-Dextran / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000378:metabolism; D005347:Fibroblasts; D000882:Haplorhini; D006367:HeLa Cells; D006801:Humans; D007372:Interferons / Q000096:biosynthesis*; D007668:Kidney; D007700:Kinetics; D007739:L Cells; D051379:Mice; D009522:Newcastle disease virus / Q000254:growth & development; D011070:Poly I-C / Q000494:pharmacology; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D013045:Species Specificity; D014357:Trypsin / Q000378:metabolism
D012333:RNA, Messenger; D003609:Dactinomycin; D007372:Interferons; D003637:DEAE-Dextran; D003513:Cycloheximide; D003851:Deoxyribonucleases; D014357:Trypsin; D011070:Poly I-C