An immunological turbidimetric method for serum transferrin determination.
The described method for the turbidimetric determination of serum transferrin concentration has the following features: the ionic strength of the medium is very low, the antiserum used is purified by the manufacturer, 25 mul antiserum per determination is sufficient, excess antibody or antigen has no influence. We studied the relation between transferrin concentration and total iron binding capacity for human normal and variant transferrins. Furthermore, our results are compared with the radial immunodiffusion technique.
D006801:Humans; D005779:Immunodiffusion; D007501:Iron / Q000097:blood; D009391:Nephelometry and Turbidimetry / Q000379:methods; D009994:Osmolar Concentration; D011234:Precipitins; D011485:Protein Binding; D014168:Transferrin / Q000032:analysis*
D011234:Precipitins; D014168:Transferrin; D007501:Iron