Isolation of a strong suppressor of nonsense mutations in Bacillus subtilis.
By treatment of Bacillus subtilis MO-101-P spoA- met thr- su- with ethyl methanesulfonate, a strong suppressor strain of nonsense mutations, B. subtilis MO-101-P spoA- [met-]+thr- su+44, was isolated. This strain does not suppress phage phi 29 mutant susB47, selected on a B. subtilis strain containing the su+3 suppressor isolated by Georgopoulos. A revertant from this mutant, susB610, was isolated, being suppressed by both the su+3 and su+44 suppressor strains. The efficiency of suppression by strain su+44 is about 50%. The experiments shown in this paper suggest that strain su+44 contains an amber and strain su+3 an ochre suppressor.
D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D001426:Bacterial Proteins / Q000096:biosynthesis; D001435:Bacteriophages / Q000378:metabolism; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005816:Genetic Complementation Test; D008970:Molecular Weight; D009154:Mutation; D014176:Protein Biosynthesis; D011830:Radiation Effects; D011995:Recombination, Genetic; D013045:Species Specificity; D013489:Suppression, Genetic; D014466:Ultraviolet Rays; D014779:Virus Replication
D001426:Bacterial Proteins; D005020:Ethyl Methanesulfonate