[The interaction between DNase I and the constituent deoxyribonucleotides of DNA].
A study of the interaction between deoxyribonucleotides and DNase I (E.C. 3.1.4.5) has disclosed a greater affinity of puric deoxyribonucleotides for the enzyme. DNase I showed one binding site for dAMP with an affinity constant of 2-4 X 10(4) M(-1). MnCl2 decreases the fixation of dAMP more effectively than MgCl2.
D000227:Adenine Nucleotides / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium; D008345:Manganese; D013329:Structure-Activity Relationship
D000227:Adenine Nucleotides; D003854:Deoxyribonucleotides; D008345:Manganese; D003851:Deoxyribonucleases; D008274:Magnesium
Mise en évidence de l'interaction entre la DNase I et les désoxyribonucléotides constitutifs de l'ADN