Control of Extracellular beta-1,3-glucanase activity in a basidiomycete species.
The basidiomycete QM 806 excreted large amounts of beta-1,3-glucanase into the culture medium. Synthesis and excretion of the enzyme were triggered by a critically low concentration of carbon source. The extracellular beta-1,3-glucanase exhibited a remarkable stability. Addition of glucose or other carbon sources to a culture after consumption of the initial carbon source led to an inactivation of the extracellular beta-1,3-glucanase by an inactivating system, which could be separated from the cells. The inactivation of beta-1,3-glucanse was prevented by cycloheximide. This indicates the necessity of active protein synthesis for the inactivation process but does not prove that the inactivating system itself is a protein. Marked changes in the electrophoretic mobility and immunological properties of beta-1,3-glucanase indicate rather profound alterations of the enzyme protein in the course of inactivation.
D001487:Basidiomycota / Q000201:enzymology* / Q000378:metabolism; D002474:Cell-Free System; D002482:Cellulose / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D005959:Glucosidases / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D005779:Immunodiffusion; D011134:Polysaccharides / Q000378:metabolism; D011487:Protein Conformation; D013213:Starch / Q000378:metabolism
D005656:Fungal Proteins; D011134:Polysaccharides; D002482:Cellulose; D013213:Starch; D003513:Cycloheximide; D005959:Glucosidases; D005947:Glucose