[Stimulation of rat ovarian adenyl cyclase by methylated luteinizing hormone (LH). The effect of ethylenediaminetetraacetic acid].
A comparative study was  made of the stimulation of rat ovary adenyl cyclase by ovine luteinizing hormone (LH) and its methylated drrivative (obtained by reductive alkylation of the lysine residues). The apparent Km (half maximal activity) of native LH and methylated LH was similar but the maximal activity obtained with the methylated derivative was significantly higher (1.2-1.8 times). When EDTA was added to the incubation medium the maximal activity obtained with the methylated LH diminished and became even lower than that obtained with native LH.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation; D005260:Female; D007986:Luteinizing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D010053:Ovary / Q000201:enzymology*; D051381:Rats; D013329:Structure-Activity Relationship
D007986:Luteinizing Hormone; D004492:Edetic Acid; D000262:Adenylyl Cyclases; D002118:Calcium
Stimulation de l'adényl-cyclase ovarienne de ratte par l'hormone lutéinisante (LH) méthylée. Influence de l'acide éthylène-diamino-têtra-acétique