Influence of complexing agents on stability and activity.
Metal ion-complexing agents, like KCN, EDTA etc., inactivate alkaline phosphatase of pig kidney. This inactivation is reversible at low concentrations of the complexing agents and irreversible at high concentrations. The reversible inhibition is probably due to removal of Zn2+ ions from the active site, where they are necessary for catalytic action, whereas the irreversible inhibition results from the removal of Zn2+ ions necessary for preservation of the structure. The inactivation is pseudo-first order. It depends on the concentration, size and charge of the complexing agents. Beta-Glycerophosphate and Mg2+ ions protect the enzyme from inactivation by complexing agents. Quantitative examination of the effect of substrate leads to a model that is similar to the "sequential model" proposed by D.E. Koshland, G. Nemethy & D. Filmer (1966) (Biochemistry 5, 365-385) to explain allosteric behavior of enzymes. It describes the sequential addition of two substrate molecules at two active centres of the dimer enzyme. The binding of the substrate molecules is accompanied by changes in the conformation, which lead to stabilization of the enzyme against attack by complexing agents.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D003486:Cyanides / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005994:Glycerophosphates; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D013552:Swine; D015032:Zinc / Q000494:pharmacology
D002614:Chelating Agents; D003486:Cyanides; D005994:Glycerophosphates; D004492:Edetic Acid; D000469:Alkaline Phosphatase; D008274:Magnesium; D015032:Zinc; D003545:Cysteine