Specific ion concentration as a factor in barotolerant protein synthesis in bacteria.
The degree of barotolerance exhibited by Pseudomonas fluorescens and Pseudomonas bathycetes in vitro polyphenylalanine-synthesizing systems can be modified by altering the concentrations of specific ions in the reaction mixture. Hybrid-protein-synthesizing systems, utilizing all the possible S-100 supernatant fluid and ribosome combinations from Escherichia coli, P. fluorescens, and P. bathycetes, were tested for barotolerance under conditions of low (16 mM Mg2+ plus 0 mM Na+) and high (150 mM Na+ plus 60 mM Mg2+) ion concentrations. The results reveal that barotolerant synthesis is a characteristic determined by the origin of the ribosome. Systems utilizing E. coli ribosomes are barosensitive at both low and high ion concentrations, P. fluorescens ribosomes barotolerant under both conditions, and P. bathycetes ribosomes barosensitive at low and barotolerant at high ion concentrations. Therefore, certain concentrations of specific ions will increase barotolerance, but only if the ribosomes are capable of functioning at high pressures.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002118:Calcium / Q000494:pharmacology*; D002474:Cell-Free System; D004926:Escherichia coli / Q000378:metabolism*; D006874:Hydrostatic Pressure; D008274:Magnesium / Q000494:pharmacology*; D009994:Osmolar Concentration; D010649:Phenylalanine / Q000096:biosynthesis; D011072:Poly U / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology*; D011312:Pressure; D011549:Pseudomonas / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D012964:Sodium / Q000494:pharmacology*
D001426:Bacterial Proteins; D011072:Poly U; D010649:Phenylalanine; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium