title: Immunoglobulin E (IgE) and IgE-containing cells in human gastrointestinal fluids and tissues.
abstract: Human gastric, small intestinal, colonic and rectal mucosae were examined for IgE-containing cells by single- and double-antibody immunofluorescence techniques, and IgE in intesinal fluids was measured by a double-antibody radioimmunoassay. IgE-containing cells were identified in all tissue specimens and comprised about 2% of all immunoglobulin-containing cells. Although less numerous than cells containing IgA, IgM or IgG, they were remarkably numerous in relation to the concentration of IgE in serum (about 0-001% of total immunoglobulin). IgE immunocytes were significantly more numerous in stomach and proximal small bowel than in colon and rectum, and were very numerous at bases of gastric and duodenal peptic ulcers. Measurable IgE was found in seventy-eight of eighty-five (92%) intestinal fluids. Sucrose gradient ultracentrifugation analysis of four of the fluids revealed that the immunologically reactive IgE was largely in fractions corresponding to molecules of lower molecular weight than that of albumin, which suggests that IgE in gut contents is degraded by proteolytic enzymes. The presence of IgE-forming cells in gastrointestinal tissues, and IgE or a fragment of IgE in intestinal fluids, suggests that IgE antibodies are available for participation in local reaginic-type reactions in the gut.
mesh_terms: D000328:Adult; D000921:Antibody-Producing Cells; D003106:Colon / Q000276:immunology; D003424:Crohn Disease / Q000276:immunology; D004381:Duodenal Ulcer / Q000276:immunology; D005753:Gastric Mucosa / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007073:Immunoglobulin E / Q000032:analysis*; D007413:Intestinal Mucosa / Q000276:immunology; D007419:Intestinal Secretions / Q000276:immunology*; D007422:Intestines / Q000276:immunology*; D008970:Molecular Weight; D011863:Radioimmunoassay; D012007:Rectum / Q000276:immunology; D013270:Stomach / Q000276:immunology*; D013276:Stomach Ulcer / Q000276:immunology
chemical_list: D007073:Immunoglobulin E