Ultrastructural study of Plasmodium knowlesi antigen used in vaccination of rhesus monkeys.
Material from various steps obtained in the French pressure cell technic of preparing antigen from Plasmodium knowlesi-infected red cells, was examined by elctron microscopy. A positively charged colloidal iron solution was used to differentiate between membranes of host red cells and parasites. Red cell membranes take the stain, wheras parasite membranes do not. This antigen which has been used previously to protect monkeys against P. knowlesi appears to consist almost entirely of membrane-bounded vesicles. Some of these vesicles contain a fine granular material, whereas others appear empty. The antigen failed to stain with the positively charged iron solution, which suggests that it is free of contamination by host cell membrane.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D004912:Erythrocytes / Q000469:parasitology; D000882:Haplorhini; D008253:Macaca mulatta; D008288:Malaria / Q000276:immunology; D010961:Plasmodium / Q000276:immunology* / Q000648:ultrastructure; D014611:Vaccination
D000941:Antigens