Multiliter production and immunochemical cross-reactivity of plant tissue culture antigens.
Cells of short ragweed suspension culture were successfully propagated in a multiliter fermentor. Proteins were isolated from tissue cultures (short ragweed, cantaloupe, marigold, and Ammi) and plants (short ragweed pollen, cantaloupe fruit, and marigold flowers) by ammonium sulfate precipitation, cross-linked dextran gel filtration, and anion-exchange chromatography. Some fractions elicited allergenic responses in ragweed-sensitive patients but not in ragweed-nonsensitive control patients. Double-immunodiffusion analysis of these fractions showed their nonidentity with short ragweed antigen E.
D000485:Allergens / Q000302:isolation & purification; D000818:Animals; D000918:Antibody Specificity; D000941:Antigens; D003429:Cross Reactions; D046508:Culture Techniques; D006801:Humans; D007120:Immunochemistry; D005779:Immunodiffusion; D010940:Plant Proteins / Q000032:analysis; D010944:Plants; D011817:Rabbits / Q000276:immunology; D012882:Skin Tests
D000485:Allergens; D000941:Antigens; D010940:Plant Proteins