title: Evolution and utility of a Pseudomonas aeruginosa drug resistance factor.
abstract: We describe the addition to the Pseudomonas aeruginosa sex factor, FP2, of carbenicillin resistance encoded by the RP1 plasmid. This occurred in a step-wise manner as detected by variations in the characteristics of the FP2-RP1 plasmid aggregate. The addition of the carbenicillin resistance marker to FP2 facilitates estimates of FP2 transfer. Transfer frequencies for the presumed cointegrate plasmid, using carbenicillin selection, approached 10(-1) per donor bacterium. The chromosomal mobilization properties of the derived plasmid, designated pR0271, resembled those of the progenitor plasmid FP2. Plasmid pR0271 was also observed to mobilize a nontransmissible drug resistance plasmid sharing genetic homology at frequencies corresponding to those observed for chromosomal markers proximal to the origin of transfer.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002228:Carbenicillin / Q000494:pharmacology; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D005144:F Factor; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D011815:R Factors; D014161:Transduction, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D002228:Carbenicillin