Distribution of [1,2-3H]cholesterol in mouse brain after injection in the suckling period.
Glutaraldehyde-carbohydrazide polymer (GACH) was used to embed olfactory tracts, trapezoid body, and sciatic nerves of 9-, 10-, and 49-day old mice 2 h, 24 h, and 6 wk (respectively) after the intraperitoneal administration of [1,2-3H]cholesterol. Greater than 94% of radioactive cholesterol was retained in the GACH-infiltrated brain 24 h or more after injection. The fine structural preservation of both central and peripheral nervous tissues was excellent. Quantitative analysis of electron microscope autoradiographs demonstrated that [1,2-3H]cholesterol is limited to blood vessel walls and lumen within the central nervous system at 2 h and 6 wk postinjection, but neurons and neuropil also contain the labeled cholesterol. The thickest myelin sheaths in the adult mice appear to be uniformly labeled throughout their width. No relationship of the retained [1,2-3H]cholesterol to the node of Ranvier was found in the adult sciatic nerve.
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography / Q000379:methods; D002490:Central Nervous System / Q000378:metabolism*; D002536:Cerebral Arteries / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D004727:Endothelium / Q000378:metabolism; D005976:Glutaral; D006652:Histological Techniques; D007274:Injections, Intraperitoneal; D051379:Mice; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009833:Olfactory Pathways / Q000378:metabolism*; D011149:Pons / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D002784:Cholesterol; D005976:Glutaral