The species distribution of three concanavalin A-purified mycobacterial polysaccharides.
Culture filtrates prepared from 12 species of mycobacteria were examined for the presence of 3 mycobacterial polysaccharides previously purified by concanavalin A affinity chromatography. Four commercial Old Tuberculins, tuberculin tine test units, and purified protein derivative were also studied. All 3 polysaccharides were demonstrated in all of the filtrates and each of the Old Tuberculins and purified protein derivative. The amount of concanavalin A-nonreactive polysaccharide was measured in clinical skin testing antigens and found to be in the range of potential significance as a cause of false positive reactions to Old Tuberculin.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D003208:Concanavalin A; D006168:Guinea Pigs; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D009161:Mycobacterium / Q000032:analysis*; D009162:Mycobacterium avium / Q000032:analysis; D009163:Mycobacterium bovis / Q000032:analysis; D009169:Mycobacterium tuberculosis / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D013045:Species Specificity; D014373:Tuberculin / Q000032:analysis*
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; D014373:Tuberculin; D003208:Concanavalin A