title: Methotrexate analogs. 6. Replacement of glutamic acid by various amino acid esters and amines.
abstract: A series of methotrexate (MTX) analogs was prepared in which the glutamic acid moiety is replaced by various amino acid esters and amines. The synthetic method consisted of the reaction of 4-amino-4-deoxy-N10-methylpteroic acid with various reagents to form intermediate mixed anhydrides, which then reacted with amino acid esters or amines to give the MTX analogs. These compounds were tested for antibacterial activity against Streptococcus faecium and for antitumor activity against L1210 leukemia in mice. Several compounds showed significant antibacterial activity; the MTX homocysteinethiolactone and MTX aspartate analogs showed marginal in vivo antitumor activity.
mesh_terms: D000818:Animals; D013293:Enterococcus faecalis / Q000187:drug effects; D005971:Glutamates; D007939:Leukemia L1210 / Q000188:drug therapy; D008727:Methotrexate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D008815:Mice, Inbred Strains; D013329:Structure-Activity Relationship
chemical_list: D005971:Glutamates; D008727:Methotrexate