[Molecular weight heterogeneity of isolated antigenic complexes and their immunochemical activity].
Antigenic complexes extracted from S. typhi Ty24446 in tryptic proteolysis displayed heterogeneity by molecular weight. The O-antigenic activity of the preparation was associated with the components with a mol. weight of 6.7-10(4)--2-10(6), Vi-antigenic activity--with the components with a mol. weight of 6.7-10(4)--2-10(6) and the components with a mol. weight of 1.75-10(4)--6.7-10(6). Components with a mol. weight below 1.75-10(4) possessed no Vi-antigenic activity. Correlations between the high- and low-molecular components in the "tryptic" antigen depended on the regimen of proteolysis.
D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D004755:Enterobacteriaceae / Q000276:immunology; D005779:Immunodiffusion; D008970:Molecular Weight; D012485:Salmonella typhi / Q000276:immunology; D014586:USSR; D014461:Ultracentrifugation
D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial
Molekuliarno-vesovaia geterogennost' izolirovannykh antigennykh kompleksov i ikh immunokhimicheskaia aktivnost'