title: Effects of aliphatic chlorohydrocarbons on drug-metabolizing enzymes in rat liver in vivo.
abstract: 1. Various polychlorinated hydrocarbons were administered intragastrically to rats to examine their effects on the biotransformation capacity of the liver. Due to high toxicity, 1,1,2,2-tetrachloroethane and pentachloroethane were given at a dose level equivalent to one quarter of that of CCl4 and the other chlorohydrocarbons (i.e. 2-6 mmol/kg). 2. Carbon tetrachloride at 10-3 mmol/kg was the most active in decreasing cytochrome P-450 content and the overall drug hydroxylation activities in rat liver. 1,1,2,2-Tetrachloroethane was the next most active in decreasing the hepatic drug oxidizing enzymic activities. 3. Expoxide hydratase activity in rat liver declined significantly after CCl4, 1,1,2,2-tetrachloroethane and pentachloroethane administrations. 4. UDP-Glucuronosyltransferase was affected to a lesser extent than the microsomal mono-oxygenase or epoxide hydratase by chlorohydrocarbon treatment.
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004072:Digitonin / Q000494:pharmacology; D004851:Epoxide Hydrolases / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D006843:Hydrocarbons, Chlorinated; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases; D009251:NADPH-Ferrihemoprotein Reductase; D005964:Glucosyltransferases; D004851:Epoxide Hydrolases; D004072:Digitonin