Characterization of human anti-factor VIII antibodies purified by immune complex formation.
Anti-factor VIII antibodies from three hemophilia A patients were selectively isolated by immune complex formation and secondary precipitation with polyethylene glycol. These antibodies were characterized as IgG, with only one type of light chains (kappa) and a mixture of IgG3 and IgG4 heavy chains. This finding indicated that stable complexes of factor VIII and homologous anti-factor VIII antibodies could be obtained, and that the immunologic response to the infusion of homologous factor VIII was oligoclonal rather than monoclonal in these patients.
D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex; D005169:Factor VIII / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007147:Immunoglobulin Light Chains / Q000032:analysis; D011092:Polyethylene Glycols / Q000494:pharmacology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D011092:Polyethylene Glycols; D005169:Factor VIII