Passive hemagglutination reaction test using formalinized sheep erythrocytes treated with tannic acid and coated with proteast or elastase of Pseudomonas aeruginosa.
Methods were established for estimating antibodies of protease and elastase of Pseudomonas aeruginosa by passive hemagglutination (HA) tests using fixed sheep erythrocytes coated with each of the two enzymes. High antibody titers of protease and elastase were found in the HA tests on sera in some cases suffered from P. aeruginosa infection. The evaluation of the HA tests was discussed.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002417:Cattle; D004731:Endotoxins / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D007106:Immune Sera; D008414:Mastitis, Bovine / Q000276:immunology; D010196:Pancreatic Elastase / Q000276:immunology*; D010447:Peptide Hydrolases / Q000276:immunology*; D011552:Pseudomonas Infections / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000276:immunology; D012141:Respiratory Tract Infections / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013634:Tannins / Q000494:pharmacology*
D000907:Antibodies, Bacterial; D004731:Endotoxins; D047348:Hydrolyzable Tannins; D007106:Immune Sera; D013634:Tannins; D010447:Peptide Hydrolases; D010196:Pancreatic Elastase