Rapid diagnosis of Venezuelan equine encephalomyelitis by fluorescence microscopy.
Goat Venezuelan equine encephalomyelitis (VEE) antiserum and normal serum were conjugated and evaluated for staining sensitivity and specificity. Cross-staining with either eastern or western equine encephalomyelitis virus-infected cells did not occur. The baby hamster kidney (BHK-21) cell line when combined with highly specific VEE conjugate detected 100 medium suckling mouse intracerebral lethal doses (suckling mouse LD-50/IC) of the 1B subtype of VEE virus per milliliter of equine tissue suspension. Conjugated goat antiserum was assayed for sensitivity for detection of VEE virus-infected equine serums and tissue suspensions. The BHK-21 cell line was superior to either primary duck embryo fibroblast (DEF) cells or African green monkey kidney (Vero) cells for propagating the GJ9-1BJ subtype of VEE virus.
D000818:Animals; D001921:Brain / Q000382:microbiology; D002478:Cells, Cultured; D006224:Cricetinae; D004372:Ducks; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology* / Q000302:isolation & purification; D004683:Encephalomyelitis, Equine / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006041:Goats / Q000276:immunology; D000882:Haplorhini; D006734:Horse Diseases / Q000175:diagnosis* / Q000382:microbiology; D006736:Horses; D007668:Kidney; D051379:Mice