[Study of oxidative enzymes of the lignin-degrading fungus Pleurotus ostreatus].
The elctrophoretic separation in polyacrylamide gel of laccase and peroxidase isoenzymes of the lignin-degrading fungus Pleurotus ostreatus was investigated. The optimal electrophoretic conditions were found: in the electrode buffer tris-diethyl barbituric acid pH 7.0 in the gradient gel-4-10% acrylamide. Seven peroxidase isoenzymes oxidizing base benzidine and guaiacol were identified and five laccase isoenzymes reacting with specific substrates-p-phenylene diamine, alphs-naphthol, pyrogallol, hydroquinone were determined.
D000363:Agaricales / Q000201:enzymology*; D004156:Catechol Oxidase / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism
D007527:Isoenzymes; D008031:Lignin; D004156:Catechol Oxidase; D010544:Peroxidases
Izuchenie okislitel'nykh fermentov ligninrazrushaiushchego griba Pleurotus ostreatus