[Possibilities of chemical germ-conservation and its meaning for the bacteriological urine diagnosis].
To estimate the germ-preserving effect of 15 percent disodium-calcium-ethylene-diamine-tetraacetate, 1.8 percent boric acid, 20 percent dimethyl sulfoxide and 3 solutions containing 0.001 percent merthiolate and 15 percent glycerin, 2 percent ethylene-diamine-tetraacetic acid and 15 percent glycerin, and 30 percent NaCl and 3 percent polyvinyl pyrrolidone respectively, comparative investigations were conducted in 4 series comprising a total of 400 urine samples. Boric acid, which is very easy to use, proved the most effective. After 24-h storage at room temperature of boric-acid-preserved urine samples with bacterial counts of over 10-4 per ml, the results were false-negative in 2-4 percent; after 48 h the rate was 6-8 percent and after 72 h nearly 10 percent. There were no false-positive results.
D001431:Bacteriological Techniques; D003470:Culture Media; D004755:Enterobacteriaceae / Q000254:growth & development; D004926:Escherichia coli / Q000254:growth & development; D005188:False Negative Reactions; D011309:Preservation, Biological; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D013210:Staphylococcus / Q000254:growth & development; D013696:Temperature; D013997:Time Factors; D014556:Urine / Q000382:microbiology*
D003470:Culture Media
Möglichkeiten der chemischen Keimkonservierung und ihre Bedeutung für die bakteriologische Urindiagnostik