Analysis of gene expression in regenerating rat liver by hybridization of nuclear and cytoplasmic RNA with DNA.
To determine whether massive gene activation occurs in rat liver following partial hepatectomy, DNA-RNA hybridization-saturation and RNA depletion experiments were performed. RNA was extracted from whole cells, nuclei, post-mitochondrial extracts, and polysomes obtained from livers of normal, sham-operated, and partially hepatectomized rats. The purified RNA was labeled with [3H]dimethyl sulfate in vitro and hybridized with nuclera DNA under conditions in which only repetitive sequence transcripts form hybrids with DNA. For comparative purposes, experiments were also performed with nuclear RNA labeled with [32P3phosphoric acid in vivo. The following observations were made: (a) for whole-cell RNA the saturation levels obtained in the hybrization reaction are the same regardless of the source of RNA USED (NORMAL, SHAM-OPERATED, OR PARTIALLY HEPATECTOMIZED RATS); (B) NO DIFFERENCES IN THE SATURATION LEVELS WERE FOUND WHEN LIVER NUCLEAR RNA from these three groups of animals were used; (c) the concentration of nuclear RNA from 6-hr regenerating liver necessary to saturate the DNA is slightly higher than that of nuclear RNA obtained from normal rat liver; (d) cytoplasmic RNA from 6-hr regenerating liver saturates the DNA at a much lower concentration than that required for RNA from normal or sham-operated rats. Our results suggest that for repetitive sequence transcripts, massive "derepression" of the genome does not occur at the early stages of liver regeneration. The alterations detected reflect primarily changes in RNA concentrations rather than qualitative alterations in gene expression. Increased transport of repetitive sequence transcripts from nucleus to cytoplasm appears to take place in regenerating liver.
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration; D008297:Male; D009693:Nucleic Acid Hybridization; D011061:Poly A; D011132:Polyribosomes / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic
D012335:RNA, Ribosomal; D011061:Poly A; D012313:RNA