Redundancy of washing in the preparation of biological specimens for transmission electron microscopy.
A marine unicellular organism, human trophoblast tissue and cultured trophoblast cells of human origin have been satisfactorily preserved for electron microscopy without resort to washing either before dehydration or between different stages of fixation. The time required to fix and dehydrate a specimen using this method is 55 min.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D002843:Chromatin / Q000648:ultrastructure; D016798:Ciliophora / Q000648:ultrastructure*; D003584:Cytological Techniques; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure; D008928:Mitochondria / Q000648:ultrastructure; D012270:Ribosomes / Q000648:ultrastructure; D013048:Specimen Handling; D014327:Trophoblasts / Q000648:ultrastructure*
D002843:Chromatin