Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns.
The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.
D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D004926:Escherichia coli; D009842:Oligopeptides / Q000138:chemical synthesis / Q000302:isolation & purification*; D012343:RNA, Transfer / Q000302:isolation & purification*; D013434:Sulfenic Acids
D009842:Oligopeptides; D013434:Sulfenic Acids; D012343:RNA, Transfer