Serological diagnosis of petriellidosis (allescheriosis). I. Isolation and characterization of soluble antigens from Allescheria boydu and Monosporium apiospermum.
Soluble antigens in culture filtrates of three strains of Petriellidium boydii and three strains of Monosporium apiospermum were examined. Antigens were separated from concentrated crude filtrates by anion-exchange chromatography. A single major peak (Antigen 1), constituting a significant proportion of the total recoverable carbohydrate, was the only product isolated from each of four chromatographed filtrates. Depending on the fungus strain, Antigen 1 consisted of 90--96% carbohydrate, 3-4% protein, and 2-4% nucleic acid. Antigen 1 was found to consist of a population of molecules with a heterogeneous molecular size when assayed by gel filtration chromatography; however, isolated fractions of Antigen 1 proved to be immunologically identical when examined by Ouchterlony immunodiffusion. In addition, Antigen 1 from each strain was immunologically identical to similar preparations of Antigen 1 from the other five fungus strains. Chromatography of culture filtrates from two strains of M. apiospermum revealed a second peak (Antigen 2), which was found to consist of 70% carbohydrate, 16% protein, and 4% nucleic acid. Although Antigen 2 contained four times as much protein as Antigen 1, the two preparations were immunologically identical by immunodiffusion tests. Ion-exchange chromatography proved to be a useful procedure for isolating antigens of P. boydii and M. apiospermum from culture filtrates.
D000946:Antigens, Fungal / Q000032:analysis / Q000302:isolation & purification; D001203:Ascomycota / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D003937:Diagnosis, Differential; D005656:Fungal Proteins / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D009181:Mycoses / Q000175:diagnosis*; D009696:Nucleic Acids / Q000032:analysis; D011541:Pseudallescheria / Q000276:immunology*; D012698:Serologic Tests; D012995:Solubility; D013045:Species Specificity
D000946:Antigens, Fungal; D002241:Carbohydrates; D005656:Fungal Proteins; D009696:Nucleic Acids