[Production of sera for determining group A Streptococcus using the precipitation in gel reaction].
The sera of rabbits immunized with the culture of streptococcus, group A, treated with pepsin were studied by the immunodiffusion methods. The sera intensively reacted with the polysaccharide of the streptococcus, group A, but not with the polysaccharide of the streptococcus of A-variant and with the proteins contained in the hyfrochloric extracts of group A. Some of the sera contained antibodies to polyglycerophosphate and antigen E4 which could be eliminated by sorption of the sera with the culture of the streptococcus of A-variant treated with pepsin. Despite the sorption, individual sera reacted with the hydrochloric extracts obtained from the streptococcus, group L, or the S. aureus. The sera reacting with the polysaccharide of group A could be used for the identification of streptococcus, group A, by the method of precipitation in gel. The advantages of the method consist in its simplicity and distinct result, which can be obtained in two hours.
D000327:Adsorption; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D007106:Immune Sera; D007114:Immunization; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D010434:Pepsin A / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011817:Rabbits; D013211:Staphylococcus aureus / Q000276:immunology; D013297:Streptococcus pyogenes / Q000276:immunology*; D013997:Time Factors
D000907:Antibodies, Bacterial; D007106:Immune Sera; D011135:Polysaccharides, Bacterial; D010434:Pepsin A
Poluchenie syvorotok dlia opredeleniia streptokokka gruppy A pri pomoshchi reaktsii pretsipitatsii v gele