Genetic mapping of sporulation operons in Bacillus subtilis using a thermosensitive sporulation mutant.
A thermosensitive sporulation mutant was used to determine the order of sporulation operonsin the urs region of the Bacillus subtilis chromosome. Data from three-factor transformation crosses and three- and four-factor transduction crosses established the order metC-SPO-96(SpoII)-spo-85(SpoV)-spo-279(SpoII)-furA-ura-cysC-spo-NG1.67(SpoIII). Previously, furA was thought to lie to the right of ura and cysC to the left (Dubnau, 1970; Young and Wilson, 1972).
D000469:Alkaline Phosphatase / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D008040:Genetic Linkage; D009154:Mutation; D009876:Operon; D013171:Spores, Bacterial / Q000254:growth & development; D013696:Temperature; D014161:Transduction, Genetic; D014170:Transformation, Genetic; D014498:Uracil / Q000378:metabolism
D014498:Uracil; D000469:Alkaline Phosphatase