title: [Lethality of the dnaB thermosensitive mutation of E. coli : comparison with thymine-less death].
abstract: In a dnaB thermosensitive mutant of the bacterium E. coli, a shift to non-permissive conditions leads to the immediate cessation of DNA synthesis and results in a sizable loss of viability. Under conditions where protein synthesis is inhibited, either by addition of antibiotics (chloramphenicol, rifampicin) or by amino-acids starvation, there is a marked increase in viability. This is reminiscent of the behavior of thymine-deprived bacteria and suggests that both types of death, induced by a block in the displacement of the DNA-replicative fork, follow the same pattern.
mesh_terms: D000596:Amino Acids; D001426:Bacterial Proteins / Q000096:biosynthesis; D002701:Chloramphenicol / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004926:Escherichia coli / Q000254:growth & development; D005804:Genes, Lethal; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D013696:Temperature; D013941:Thymine
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D012333:RNA, Messenger; D002701:Chloramphenicol; D013941:Thymine; D012293:Rifampin
vernacular_title: Léthalité de la mutation thermosensible dnaB de la bactérie E. coli : comparaison avec la mort des bactéries carencées en thymine