The in vitro metabolism, macromolecular binding and bacterial mutagenicity of 4-chloribiphenyl, a model PCB substrate.
The in vitro metabolism of 4-chlorobiphenyl, a model polychlorinated biphenyl (PCB) substrate, proceeds via an arene oxide intermediate to give the observed in vivo hydroxylated metabolites. The rabbit liver microsomal fraction mediates binding between the PCB and the endogenous microsomal protein and RNA and the major part of the PCB was bound to the light 3S-10S RNA fraction. The lower chlorinated 4-chlorobiphenyl isomer was highly mutagenic to the Salmonella typhimurium strain TA1538 (sensitive to frameshift mutagens) whereas higher chlorinated PCB were only weakly mutagenic.
D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens; D011078:Polychlorinated Biphenyls / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D012313:RNA / Q000378:metabolism; D011817:Rabbits; D012486:Salmonella typhimurium / Q000187:drug effects; D013329:Structure-Activity Relationship; D013997:Time Factors
D001140:Aroclors; D001713:Biphenyl Compounds; D046911:Macromolecular Substances; D009153:Mutagens; D012313:RNA; D011078:Polychlorinated Biphenyls; C006983:4-chlorobiphenyl