The membrane location of scrapie infectivity.
Using 5'-nucleotidase and NADPH: cytochrome c reductase as respective enzyme markers for the plasma membrane and endoplasmic reticulum, a satisfactory separation of these two membrane fractions from a cell line (SMB) derived from a scrapie mouse brain has been achieved. The coincident distribution of scrapie infectivity and 5'-nucleotidase in various fractions isolated from these cells indicates that most of the scrapie infectivity present in this cell line is associated with the plasma membrane.
D000818:Animals; D001921:Brain; D002458:Cell Fractionation; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology / Q000382:microbiology; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000382:microbiology; D051379:Mice; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009708:Nucleotidases / Q000378:metabolism; D011328:Prions / Q000302:isolation & purification* / Q000472:pathogenicity; D013347:Subcellular Fractions / Q000201:enzymology
D011328:Prions; D009251:NADPH-Ferrihemoprotein Reductase; D009708:Nucleotidases