[Role of metal ions in beef liver isocitrate dehydrogenase activity. Characterization of substrate forms].
The enzymatic activity of cytoplasmic NADP linked isocitrate deshydrogenase is under control of divalent metal cations. Both forms of tribasic isocitrate, free and metal chelated are used as alternative substrates with different turnover and affinity. The esential role of the metal cation is to increase the affinity of the substrate for the enzyme 70 times.
D000818:Animals; D002413:Cations, Divalent; D002417:Cattle; D004492:Edetic Acid / Q000494:pharmacology; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D002413:Cations, Divalent; D008345:Manganese; D004492:Edetic Acid; D007521:Isocitrate Dehydrogenase; D008274:Magnesium; D015032:Zinc
Rôle des ions métalliques dans l'activité de l'isocitrate déshydrogénase de foie de boeuf. Caractérisation des formes du substrat