[Concentration, purification, and isotopic labeling of sigma virus].
Sigma virus has been concentrated by means of polyethylene-glycol and diafiltration; the latter method is more efficient in preserving infectivity. When purified on sucrose gradient, sigma banded in a sharp peak without any polydispersion. We are now able to label sigma genome with 3H-uridine in Drosophila cell cultures.
D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D004330:Drosophila / Q000382:microbiology; D007553:Isotope Labeling; D011095:Polyethylenes / Q000494:pharmacology; D012328:RNA Viruses / Q000302:isolation & purification*; D014462:Ultrafiltration; D014529:Uridine / Q000378:metabolism; D014776:Virus Cultivation
D011095:Polyethylenes; D014529:Uridine
Concentration, purification et marquage isotopique du virus sigma