F antigen. II. Chemical and physical properties.
Physical and chemical properties of the liver-specific F antigen suggested a model for the labile quarternary structure of the protein. The native molecule showed a size slightly larger than 60,000 dalton (d), which was reduced to about 40,000 d under acidic conditions. Breaking of hydrogen bonds by chaotropic treatment resulted in the release of components of 30,000, 7,000 and 2,000 d. The smallest component was split to fragments of about 1,000 d by the reducing action of sulfhydryl compounds.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis*; D001963:Bromelains; D002268:Carboxypeptidases; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002725:Chloroform; D002850:Chromatography, Gel; D002918:Chymotrypsin; D003956:Dialysis; D006801:Humans; D006861:Hydrogen Peroxide; D005779:Immunodiffusion; D007460:Iodoacetamide; D007526:Isoelectric Point; D008099:Liver / Q000276:immunology*; D008623:Mercaptoethanol; D051379:Mice; D008807:Mice, Inbred BALB C; D008961:Models, Structural; D008970:Molecular Weight; D011487:Protein Conformation; D013057:Spectrum Analysis; D014357:Trypsin
D000941:Antigens; D008623:Mercaptoethanol; D002725:Chloroform; D001963:Bromelains; D006861:Hydrogen Peroxide; D002268:Carboxypeptidases; D002918:Chymotrypsin; D014357:Trypsin; D007460:Iodoacetamide