title: Use of indirect microhemagglutination test with stable sensitized red cells in the detection of horse protein allergy.
abstract: Sheep red blood cells were stabilized with sulfosalicylic acid and sensitized with purified horse-IgG by the action of glutardialdehyde. The cells were used in an indirect microhemmagglutination test with serum samples from 12 rabbits immunized with horse-IgG and complete Freund's adjuvant as well as from 10 control animals. 32 human serum samples were examined, 6 of them from patients with established horse protein allergy. The results were compared to those of a standard method of indirect hemagglutination using fresh human erythrocytes (group O Rh-), an active hemagglutination test against horse erythrocytes, Ouchterlony's immunodiffusion and skin tests with horse-IgG. The method proved to be very sensitive. The results correlated well with those of the other immunological techniques. The stable sensitized cells did not lose sensitivity after 1 year of storage at 4 degrees C.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D004356:Drug Storage; D004912:Erythrocytes / Q000276:immunology*; D005976:Glutaral / Q000378:metabolism; D006386:Hemagglutination Tests / Q000379:methods*; D006736:Horses / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000378:metabolism; D007273:Injections, Intramuscular; D011817:Rabbits; D012756:Sheep; D012882:Skin Tests
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D005976:Glutaral