title: 3'-Terminal nucletide sequence (n equals 361) of bacteriophage MS2 RNA.
abstract: 32P-Labeled MS2 RNA was partially digested with ribonuclease T1 (guanyloribonuclease; ribonucleate 3'-guanylo-oligonucleotidohydrolase; EC 3.1.4.8) or with epilson-carboxymethyl-lysine-41 pancreatic ribonuclease A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase; EC 3.1.4.22). A series of overlapping fragments was obtained which allowed the reconstruction of a 361-nucleotide-long 3'-terminal sequence. A unique reading frame could be deduced, which indicated that the replicase gene ends with a U-A-G termination signal and is followed by a 174-nucleotide-long untranslated segment.
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D003090:Coliphages / Q000032:analysis*; D004720:Endonucleases; D009690:Nucleic Acid Conformation; D010179:Pancreas / Q000201:enzymology; D012328:RNA Viruses / Q000032:analysis; D012367:RNA, Viral; D006163:Ribonuclease T1; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis
chemical_list: D012367:RNA, Viral; D012265:Ribonucleotides; D004720:Endonucleases; D012260:Ribonucleases; D006163:Ribonuclease T1