Demonstration of carbohydrate structures in malignant melanoma tyrosinase.
Electrophoretic studies on malignant melanoma extracts before and after treatment with neuraminidase revealed that tyrosinase is a glycoprotein containing N-acetyl-neuraminic acid. Double diffusion tests using Concanavalin A and the lectin from Ricinus communis show that the carbohydrated chain of tyrosinase contains D-mannose as a sugar unit located within the carbohydrate chain. The terminal neuraminic acid groups are linked to D-galactose. The enzymatic activity of tyrosinase is not inhibited by Concanavalin A.
D002241:Carbohydrates / Q000032:analysis*; D004156:Catechol Oxidase / Q000032:analysis*; D005690:Galactose / Q000032:analysis; D006023:Glycoproteins; D006801:Humans; D005779:Immunodiffusion; D037102:Lectins; D008358:Mannose / Q000032:analysis; D008545:Melanoma / Q000201:enzymology*; D012794:Sialic Acids / Q000032:analysis
D002241:Carbohydrates; D006023:Glycoproteins; D037102:Lectins; D012794:Sialic Acids; D004156:Catechol Oxidase; D008358:Mannose; D005690:Galactose