Formation of lipid-bound N-acetylhexosamine derivatives in yeast particulate fractions.
1. Particulate fraction (105 000 g) from Saccharomyces cerevisiae catalyses the transfer of N-acetylglucosamine from UDP-N-acetyl[14 C] glucosamine into lipid fraction as well as to insoluble polymer. 2. The evidence presented is in favour of the lipid containing the N-acetylglucosamine mono-, di- and tri-saccharide derivatives of dolichyl diphosphate. 3. The presence of a transferase synthesizing dolichyl-linked sugars in mitochondrial fraction is also reported.
D000117:Acetylglucosamine / Q000378:metabolism*; D005646:Fucosyl Galactose alpha-N-Acetylgalactosaminyltransferase / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives*; D050356:Lipid Metabolism; D008928:Mitochondria / Q000201:enzymology; D008970:Molecular Weight; D011108:Polymers; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000648:ultrastructure
D011108:Polymers; D005646:Fucosyl Galactose alpha-N-Acetylgalactosaminyltransferase; D005944:Glucosamine; D000117:Acetylglucosamine