Quantitative estimation of secretory immunoglobulin A by radial immunodiffusion technique.
A single radial diffusion system in agar gel, which can be used for the quantitative measurement of secretory immunoglobulin A in solutions and in biological fluids, is discussed. Concentrations of secretory immunoglobulin A as low as 0.095 mg/ml were measured successfully using a 2 mul sample volume. Conditions that achieve maximum sensitivity and precision, such as gel concentration, incubation time, and temperature, are described.
D005260:Female; D006801:Humans; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis*; D007700:Kinetics; D008832:Microchemistry; D008894:Milk Proteins / Q000032:analysis*; D008895:Milk, Human / Q000032:analysis; D011247:Pregnancy; D013696:Temperature
D007070:Immunoglobulin A; D008894:Milk Proteins