title: Serological studies on the carbohydrate moiety of human tyrosinase.
abstract: 1. Human tyrosinase is a glycoprotein containing N-acetylneuraminic acid. 2. The terminal neuraminic acid groups are linked to D-galactose. 3. In addition, human tyrosinase reacts strongly with concanavalin A. This finding indicates that the carbohydrate chain of human tyrosinase contains D-mannose. 4. The data presented here suggest that the carbohydrate moiety of human tyrosinase is linked to the protein core in an alkali-stable form. 5. Alkali-labile carbohydrate chains which occur predominantly in membrane bound glycoproteins could not be demonstrated serologically.
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis; D000373:Agglutinins; D002241:Carbohydrates / Q000032:analysis*; D004156:Catechol Oxidase / Q000032:analysis*; D005690:Galactose / Q000032:analysis; D006023:Glycoproteins; D006801:Humans; D005779:Immunodiffusion; D007518:Isoantibodies; D037102:Lectins; D008358:Mannose / Q000032:analysis; D009439:Neuraminidase; D011108:Polymers; D012794:Sialic Acids / Q000032:analysis
chemical_list: D000373:Agglutinins; D002241:Carbohydrates; D006023:Glycoproteins; D007518:Isoantibodies; D037102:Lectins; D011108:Polymers; D012794:Sialic Acids; D004156:Catechol Oxidase; D009439:Neuraminidase; D008358:Mannose; D000117:Acetylglucosamine; D005690:Galactose