title: Formation of dry-heat resistant Bacillus subtilis var. niger spores as influenced by the composition of the sporulation medium.
abstract: Bacillus subtilis var. niger spores were produced on 20 different media. The spore yield from each medium and the dry-heat resistance at 160 C of the different spore populations were determined. The yield varied with a factor of 10(6) and the variation in D 160-value was about 10-fold (less than 20 S-190 S). A "synthetic" medium producing a high yield of spores with high dry-heat resistance was formulated. The concentrations of glucose, sucrose and calcium were found to be critical.
mesh_terms: D001412:Bacillus subtilis / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D013171:Spores, Bacterial; D013395:Sucrose / Q000378:metabolism
chemical_list: D003470:Culture Media; D013395:Sucrose; D005947:Glucose; D002118:Calcium