The detection of doxycycline activity in human bone.
Measurements of antibacterial activity in bone from 25 patients undergoing orthopaedic surgery were performed after preoperative treatment with doxycycline in a single dose of 200 mg. The antibiotic was given by intravenous infusion during 30-40 minutes, and the operation was performed 2-6 hours after administration of the dose. Using the agar diffusion method, the concentrations ranged from 0.05-0.6 mug/g. Using the electrophoretic method, higher levels of doxycycline were found, possibly as a consequence of a diminution in the binding capacity of the bone.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001424:Bacterial Infections / Q000517:prevention & control; D001681:Biological Assay; D001842:Bone and Bones / Q000032:analysis / Q000378:metabolism*; D004318:Doxycycline / Q000032:analysis / Q000378:metabolism* / Q000627:therapeutic use; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D006882:Hydroxyapatites / Q000032:analysis; D005779:Immunodiffusion; D008297:Male; D008875:Middle Aged; D013530:Surgical Wound Infection / Q000517:prevention & control
D006882:Hydroxyapatites; D004318:Doxycycline