Human erythrocyte rosette formation with mitogen-stimulated human lymphocytes--a marker for the demonstration of activated T-cells.
Human lymphocytes after stimulation by various mitogens could be shown to make rosettes with washed uncoated human Group O rhesus negative erythrocytes (H-rosettes). Using purified T- and B-cell populations and exposing them to the action of mitogens it has been demonstrated that the cell responsible is the activated T-lymphocyte. The H-rosette is thus presented as a new marker for the demonstration of activated human T-cells.
D000818:Animals; D001345:Autoradiography; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007104:Immune Adherence Reaction; D037102:Lectins; D008213:Lymphocyte Activation; D008934:Mitogens; D012204:Rh-Hr Blood-Group System; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D037102:Lectins; D008934:Mitogens; D012204:Rh-Hr Blood-Group System; D014316:Tritium; D013936:Thymidine