[Rat liver polysomes bound to endoplasmic reticulum: evidence for an RNase sensitive ribosome-membrane linkage].
Repeated low speed centrifugations of a post-mitochondrial supernatant from rat liver through discontinuous sucrose gradients in presence of cytoplasmic RNase inhibitor yield a purified rough endoplasmic reticulum fraction. This fraction contains loosely-associated ribosomes (up to 20%) which can be released from membranes by a slight RNase treatment.
D000818:Animals; D001665:Binding Sites; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D008099:Liver / Q000648:ultrastructure; D008297:Male; D009994:Osmolar Concentration; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D012260:Ribonucleases; D012270:Ribosomes / Q000378:metabolism*
D012333:RNA, Messenger; D012260:Ribonucleases
Polysome liés au réticulum endoplasmique d'hépatocyte de Rat: mise en évidence d'une liaison ribosome-membrane sensible à l'action très ménagée de la RNase.