[Biological properties and chemical composition of a soluble fraction of Brucella abortus. Monospecific A or M antigens].
ABS, the supernatant of smooth B. abortus phenol-protected suspensions, is a protein, lipid, sugar and ARN complex containing all amino acids found in phenol-water fractions from B. abortus and B. melitensis. ABS is non-toxic and immunizes mice against B. abortus challenge. Chromium chloride easily binds ABS to sheep erythrocytes (E ABS) for a specific and accurate passive hemagglutination test in brucellosis. E ABS are agglutinated by all antisera to fractions of B. abortus or B. melitensis, but not by the monospecific anti M serum. A study of the antigenic relationships between ABS and phenol-water fractions from smooth brucella strains leads to the isolation of sub-fractions fron the phenol precipitates of B. abortus or of B. melitensis that could contain only A or M antigens which react only with their respective monospecific A or M serum.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis; D002002:Brucella / Q000166:cytology / Q000276:immunology; D002004:Brucella Vaccine; D002003:Brucella abortus / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D002458:Cell Fractionation; D006386:Hemagglutination Tests; D008055:Lipids / Q000032:analysis; D051379:Mice; D012329:RNA, Bacterial / Q000032:analysis; D012995:Solubility
D000596:Amino Acids; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002004:Brucella Vaccine; D002241:Carbohydrates; D008055:Lipids; D012329:RNA, Bacterial
Propriétés biologiques et composition chimique d'une fraction soluble (ABS) de Brucella abortus. mise en évidence des antigènes monospécifiques A ou M