Initiation of phospholipase A2 activity in human platelets by the calcium ion ionophore A23187.
Phospholipase A2 activity in a suspension of intact platelets was induced by addition of a Ca2+-ionophore, A23187. This action of ionophore A23187 was enhanced by EGTA and inhibited by an excess of Ca2+ in the medium, findings which suggest that A23187 is able to mobilize intracellular Ca2+ for the latter's role as an essential cofactor for phospholipase A2 activity.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001095:Arachidonic Acids / Q000097:blood; D001792:Blood Platelets / Q000201:enzymology*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D006801:Humans; D010101:Oxygen Consumption / Q000187:drug effects; D010740:Phospholipases / Q000097:blood*; D013917:Thrombin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001095:Arachidonic Acids; D000001:Calcimycin; D004533:Egtazic Acid; D010740:Phospholipases; D013917:Thrombin; D002118:Calcium