[Comparative evaluation of methods for research on Australia antigen].
490 blood samples have been examined for the comparison of the various methods concerned with the detection of the Australia Antigen (Au). The following methods have been comparatively examined: immunodiffusion (ID) counterelectrophoresis (CEP), complement fixation (CF) passive hemoagglutination (HA) and solid phase radioimmunoassay RIA. On the basis of the results so far obtained the RIA appears the most sensitive and specific method, followed by the HA. In order to obtain reproducible results for diagnostic purposes we suggest to carry out routinely two different techniques together (possibly RIA and HA), while for screening purposes just one technique, choosed between the two most indicative ones, shall be employed.
D000917:Antibody Formation; D001782:Blood Donors; D006385:Hemagglutination Inhibition Tests; D006511:Hepatitis B Antigens / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D005779:Immunodiffusion; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay
D006511:Hepatitis B Antigens; D007457:Iodine Radioisotopes
Valutazione comparativa di metodi di ricerca dell'antigene Australia