R factor-mediated resistance to aminoglycoside antibiotics in Pseudomonas aeruginosa.
Conjugal transferability of drug resistance was examined, in eleven Pseudomonas aeruginosa strains which were isolated in Frankfurt. Four R factors were demonstrated from three strains using P. aeruginosa as recipients but they were nontransferable to Escherichia coli K12. Two R factors, i.e., Rms146 and Rms147, mediated resistances to tetracycline (TC), streptomycin (SM), sulfanilamide (SA), kanamycin (KM), lividomycin (LV), gentamicin C complex (GM) and 3', 4'-dideoxykanamycin B (DKB). They mediated the formation of aminoglycoside-inactivating enzymes, i.e., SM phosphotransferase, SM adenylyltransferase, KM and LV phosphotransferase l, and GM and DKB 6'-N-acetyltransferase. TC resistance conferred by these R factors was due to impermeability of the drug. P. aeruginosa Ps 142 carried two kinds of R factor in one cell, Rms148 (SM) and Rms149 (SM-SA-GM-CPC) (CPC, carbenicillin). Rms148 (SM) was transferable at a high frequency of 10-1 and mediated the formation of SM phosphotransferase. Rms149 mediated the formation of drug-inactivating enzymes, ie., GM 3-N-acetyltransferase and beta-lactamase, but did not inactivate SM. SM resistance was probably due to impermeability of the drug.
D000123:Acetyltransferases / Q000378:metabolism; D000617:Aminoglycosides / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000378:metabolism; D005839:Gentamicins / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011815:R Factors; D013307:Streptomycin / Q000378:metabolism; D013424:Sulfanilamides / Q000378:metabolism; D013752:Tetracycline / Q000378:metabolism
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D005839:Gentamicins; D013424:Sulfanilamides; D000123:Acetyltransferases; D010770:Phosphotransferases; D013752:Tetracycline; D013307:Streptomycin