title: A simple method of characterizing mitochondrial ribonucleic acid.
abstract: A simple method of isolating and characterizing RNA from L-cell mitochondria is described. The mitochondrial fraction is lysed by sodium dodecyl sulphate, and the RNA fractionated by sucrose-density-gradient centrifugation. The efficacy of proteinase K in preventing ribonuclease activity is also demonstrated.
mesh_terms: D003600:Cytosol; D003609:Dactinomycin; D004492:Edetic Acid; D008928:Mitochondria / Q000032:analysis; D010447:Peptide Hydrolases; D012313:RNA / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000032:analysis; D012260:Ribonucleases
chemical_list: D012335:RNA, Ribosomal; D003609:Dactinomycin; D012313:RNA; D004492:Edetic Acid; D012260:Ribonucleases; D010447:Peptide Hydrolases