Enzymic measurement of cholesterol in serum with the CentrifiChem centrifugal analyzer.
Cholesterol is measured by mixing 5 mul of sample with 350 mul of a reagent consisting of phenol, 4-aminoantipyrine, and the enzymes cholesterol oxidase, cholesterol esterase, and peroxidase. After 12 min, the resulting quinoneimine is measured at 520 nm. Readings and cholesterol concentrations are linearly related up to 4.0 g/liter. Lipemic sera and samples containing uric acid (up to 200 mg/liter), hemoglobin (up to 1 g/liter), and certain drugs (clofibrate, phenobarbital, nicotinic acid, Ketochol, Ovral-28), gave no interference. Abnormally high concentrations of bilirubin and ascorbic acid in serum lowered the cholesterol values. This enzymic assay, compared with the method of Abell and with a rate method that uses the Hantzsch reaction, gave correlation coefficients of 0.987 and 989, respectively.
D001205:Ascorbic Acid / Q000494:pharmacology; D001663:Bilirubin / Q000494:pharmacology; D002498:Centrifugation; D002784:Cholesterol / Q000097:blood*; D006735:Horseradish Peroxidase; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases; D008722:Methods; D002787:Sterol Esterase
D002784:Cholesterol; D006913:Hydroxysteroid Dehydrogenases; D006735:Horseradish Peroxidase; D002787:Sterol Esterase; D001205:Ascorbic Acid; D001663:Bilirubin