title: Isolation of rat and rabbit IgM from normal serum using anti-human mu antibody-polyacrylamide beads immunosorbents.
abstract: The antigenic relationship between human IgM and IgM from other species has been utilized in isolating rat and rabbit IgM from normal sera by means of an anti-human mu-polyacrylamide beads immunoadsorbent. The isolated IgM was found to be pure by immunoelectrophoretic analysis and Ouchterlony technique. The antisera obtained were monospecific for mu-chains after absorption with IgG. Lastly, the IgM isolated always retained its antibody activity.
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M / Q000302:isolation & purification*; D007148:Immunoglobulin mu-Chains; D008297:Male; D011817:Rabbits; D051381:Rats
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains