Apparent inactivation of inosine 5'-monophosphate dehydrogenase in sporulating Bacillus subtilis Is an artifact of in vitro proteolysis.
The intracellular levels of inosine 5'-monophosphate dehydrogenase were found to decline very slowly during stationary phase in Bacillus subtilis when adequate precautions were taken to prevent proteolysis after cell rupture.
D001412:Bacillus subtilis / Q000201:enzymology* / Q000254:growth & development; D001426:Bacterial Proteins / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D007168:IMP Dehydrogenase / Q000378:metabolism*; D007658:Ketone Oxidoreductases / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000096:biosynthesis; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011489:Protein Denaturation / Q000187:drug effects; D013048:Specimen Handling; D013171:Spores, Bacterial / Q000201:enzymology
D001426:Bacterial Proteins; D010664:Phenylmethylsulfonyl Fluoride; D004492:Edetic Acid; D007168:IMP Dehydrogenase; D007658:Ketone Oxidoreductases; D010447:Peptide Hydrolases