DNA repair after UV and gamma irradiation. I. Rat spleen cells.
An in vitro microculture was set up to standardize DNA enzymatic repair in rat spleen lymphocytes after exposure to ultraviolet or gamma rays by measuring the tritiated thymidine uptake. A relation between irradiation dose and DNA repair is always observable when cell viability and scheduled DNA synthesis are taken into account. This micromethod utilizing rat spleen cells appears to be a suitable system for such a study since it permits a satisfactory evaluation of DNA repair.
D000818:Animals; D002478:Cells, Cultured; D003584:Cytological Techniques; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects*; D005260:Female; D005720:Gamma Rays; D006918:Hydroxyurea / Q000494:pharmacology; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000378:metabolism / Q000528:radiation effects*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays
D014316:Tritium; D004247:DNA; D013936:Thymidine; D006918:Hydroxyurea