Genetic control of soluble NAD-dependent sorbitol dehydrogenase in Drosophila melanogaster.
Experiments utilizing standard techniques of cell fractionation and disc electrophoresis have revealed the presence of three distinctly different enzymes which catalyze the oxidation of D-sorbitol in crude extracts of Drosophila melanogaster adults. These include (1) a soluble NAD-dependent sorbitol dehydrogenase (NAD-SoDHS), (2) a mitochondrial NAD-dependent sorbitol dehydrogenase (NAD-SoDHm), and (3) a soluble NADP-dependent sorbitol dehydrogenase (NADP-SoDH). The structural gene for NAD-SoDHS has been mapped to a locus between 65.3 and 65.6 on the third chromosome by means of an electrophoretic variant and a low-activity allele. Through the use of segmental aneuploidy, this gene has been localized to the region limited by salivary bands 91B-93F. Because mutants which alter either the activity or electrophoretic effect on the mitochondrial or NADP-dependent forms, it is suggested that the enzymes in this system are coded for autonomously by different genes.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D004331:Drosophila melanogaster / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D007527:Isoenzymes; D007700:Kinetics; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D012469:Salivary Glands / Q000648:ultrastructure; D013045:Species Specificity
D007527:Isoenzymes; D000429:Alcohol Oxidoreductases; D007064:L-Iditol 2-Dehydrogenase