Acute cytogenetic effect of 2-(2-furyl)-3-(5-nitro-2-furyl)-acrylamide (AF-2, a food preservative) on rat bone marrow cells in vivo.
The cytogenetic effects of 2(2-furyl)-3-(5-nitro-2-furyl)-acrylamide (AF-2), a food preservative used in Japan, on rat bone marrow cells in vivo were studied. The aberrant metaphase cells in the bone marrow increased and reached the peak level 6 h after intraperitoneal injection of 240 mg/kg body weight of AF-2 and returned to the normal level within 24 h. A dose-response relationship was obtained using 4-240 mg/kg of AF-2. Chromosome aberrations were also induced after oral administration of 30-240 mg/kg. The aberrations were mostly chromatid breaks and the distribution among and within chromosomes was similar to those induced by a carcinogen, 7, 12-dimethylbenz(a)anthracene (DMBA). The present results provide the first evidence of in vivo cytogenetic effects of AF-2 on mammalian cells and, together with the evidence of mutagenicity already proved in other organisms, warn of the possible genetic hazards to cells exposed to this compounds.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D000178:Acrylamides / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D000284:Administration, Oral; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002842:Chromatids / Q000187:drug effects; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D005520:Food Preservatives; D005663:Furans / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity; D007274:Injections, Intraperitoneal; D008297:Male; D008938:Mitosis; D009153:Mutagens / Q000494:pharmacology; D009581:Nitrofurans / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D013997:Time Factors
D000178:Acrylamides; D005520:Food Preservatives; D005663:Furans; D009153:Mutagens; D009581:Nitrofurans; D015127:9,10-Dimethyl-1,2-benzanthracene