Determination of placental estriol in urine by gas-liquid chromatography, with equilenin as internal standard.
Placental estriol in urine can be determined by rapid enzymatic hydrolysis, extraction with neutral XAD-2 resin, and quantitation by gas-liquid chromatography, with equilenin as the internal standard. Glucose, methenamine mandelate, hydrochlorothiazide, phenolphthalein, or salicylate do not interfere. Analytical recovery of estriol added to urine was 92-108%. Day-to-day precison (CV), established by 15 repilicate analyses at a mean concentration of 21.3 mg/liter, was 6.6%.
D015068:17-Ketosteroids; D002849:Chromatography, Gas; D004855:Equilenin; D004964:Estriol / Q000652:urine*; D005260:Female; D006801:Humans; D008722:Methods; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy
D015068:17-Ketosteroids; D004964:Estriol; D004855:Equilenin