Fluid and solid media for isolation of Brucella abortus.
The use of a fluid-enrichment technique increased by 10-16% the recovery rate of Brucella abortus from milks of herds positive to a milk ring test. Three solid media for direct cultural techniques were also tested on the same milks. Combinations of these techniques produced a recovery rate of ca. 88% from MRT-positive-herd milks using a mixture of the centrifuged deposit and cream layer as the inoculum as against a recovery rate of ca. 75% using the MRT cream layer as inoculum. B. abortus biotypes 1-5 and 9 were isolated and growth of all the biotypes was supported by all media used.
D000818:Animals; D002002:Brucella / Q000302:isolation & purification*; D002003:Brucella abortus / Q000254:growth & development; D003470:Culture Media; D008892:Milk / Q000382:microbiology*
D003470:Culture Media