title: Effects of experimental coronary thrombosis upon regional and systemic plasma chromatographic patterns.
abstract: An evaluation of changes in the plasma chromatographic pattern was carried out in dogs with experimental coronary thrombosis. Sequential samples were taken simultaneously from the aorta and coronary sinus over a two, four and twenty-four hour period. In addition to gel-chromatography, immunodiffusion and electrophoresis were also employed to identify the protein components of the elution fractions. The results showed that there was a shift of the normal chromatographic pattern to the left from the control within two hours following thrombotic occlusion of the coronary artery and it was maintained over a twenty-four hour period. The shift to the left was presumably due to the earlier elution of the heavy molecular weight components forming as a result of the thrombotic process. The early appearance of the altered chromatographic pattern constitutes an important advantage worthy of further exploration for possible diagnostic application in the human subject.
mesh_terms: D000818:Animals; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000032:analysis; D002850:Chromatography, Gel; D003327:Coronary Disease / Q000097:blood*; D004195:Disease Models, Animal; D004285:Dogs; D005340:Fibrinogen / Q000032:analysis*; D005779:Immunodiffusion
chemical_list: D001798:Blood Proteins; D005340:Fibrinogen