Selective activation of classical and alternative pathways of human complement by "promptly serum-sensitive" and "delayed serum-sensitive" strains of Serratia marcescens.
Chelation of fresh human serum with 0.01 M MgCl2 (Mg) plus 0.01 M ethylene glycol tetraacetic acid failed to abrogate the bactericidal activity against "delayed serum-sensitive" strains of Serratia marcescens, whereas previously "promptly serum-sensitive" strains of S. marcescens and control strain Escherichia coli C were killed after an extended period of incubation. The addition of 0.01 M ethylenediametetracetate to fresh human serum neutralized bactericidal activity against S. marcescens of either serum sensitivity category.
D001770:Blood Bactericidal Activity; D002413:Cations, Divalent; D003165:Complement System Proteins / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D012706:Serratia marcescens / Q000276:immunology*; D013997:Time Factors
D002413:Cations, Divalent; D004533:Egtazic Acid; D003165:Complement System Proteins; D004492:Edetic Acid; D008274:Magnesium