The effect of parenteral alimentation fluid, undiluted with saline or fresh sera, on the growth of Candida albicans in vitro at 37 degrees C.
Parenteral alimentation is often complicated by Candida albicans infection which may be fatal. This study investigated the effect of alimentation fluid (Aminosol) on C. albicans' growth in vitro. It was found that concentrated Aminosol (1400 millisomoles) maintained C. albicans in a viable state but inhibited replication. Dilution of alimentation fluid to physiological concentrations (300 milliosmoles) with either saline or aged pooled normal sera promoted in vitro growth of C. albicans which was equivalent to that obtained in BHI broth and was slightly less than that obtained in Sabouraud's broth. The effects of fresh sera with full complement activity were also investigated. In fresh sera appropriately diluted with physiological saline, some clumping of the yeasts was observed and all formed germ tubes. Growth as defined by budding or the formation of hyphae was inhibited. When Aminosol was diluted to 300 milliosomoles with fresh sera, all yeasts were noted to be in clumps with germ tubes as well as continually growing hyphae. Growth was approximately equal to that seen in Aminosol similarly diluted with saline.
D001798:Blood Proteins; D002176:Candida albicans / Q000166:cytology / Q000254:growth & development*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D006801:Humans; D008858:Microscopy, Phase-Contrast; D010288:Parenteral Nutrition; D010289:Parenteral Nutrition, Total; D011492:Protein Hydrolysates / Q000494:pharmacology*; D012965:Sodium Chloride; D013696:Temperature; D013997:Time Factors
D001798:Blood Proteins; D003470:Culture Media; D011492:Protein Hydrolysates; D012965:Sodium Chloride