title: Adaptation of a stable L-form of Bacillus subtilis to minimal salts medium without osmotic stabilizers.
abstract: An L-form isolated from Bacillus subtilis 168 was adapted to growth in a 340 mOsm minimal salts medium without the addition of osmotically protective solutes. This L-form had no chemically detectable peptidoglycan residues on its surface, but 0.8% of the dry weight of washed membranes was hexosamine. The osmotic stability and susceptibility to bacitracin and vancomycin of the L-form adapted to growth in 340 mOsm osmotically unprotected medium was twice that of the L-form grown in 2,680 mOsm medium supplemented with 1.2 M NaCl.
mesh_terms: D000222:Adaptation, Physiological; D001412:Bacillus subtilis / Q000032:analysis / Q000187:drug effects / Q000254:growth & development*; D001414:Bacitracin / Q000494:pharmacology; D002462:Cell Membrane / Q000032:analysis; D003523:Cycloserine / Q000494:pharmacology; D006595:Hexosamines / Q000032:analysis; D007740:L Forms / Q000032:analysis / Q000187:drug effects / Q000254:growth & development*; D009994:Osmolar Concentration; D010400:Penicillin G / Q000494:pharmacology; D012965:Sodium Chloride; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D006595:Hexosamines; D001414:Bacitracin; D012965:Sodium Chloride; D014640:Vancomycin; D003523:Cycloserine; D010400:Penicillin G