Rat polyvinyl sponge model for the study of infections: initial investigations.
Polyvinyl sponges were implanted subcutaneously on both sides of young female rats. One sponge was infected with 10(8) of either Escherichia coli K-12 F-, Staphylococcus aureus ATCC 25923, or Pseudomonas aeruginosa CDC 7725. P. aeruginosa remained at the inoculum level and S. aureus declined by 1 log, whereas E. coli was reduced 1,000-fold. Only P. aeruginosa was recovered from the blood in 36% of the animals in 24 h and in 20% of the rats in 48 h. The nutrient potential of rat inflammatory fluid was compared to nutrient broth by growth of each bacterium in untreated and heat-inactivated sponge fluids and Trypticase soy broth.
D000818:Animals; D003470:Culture Media; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000254:growth & development / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D006321:Heart / Q000382:microbiology; D007958:Leukocyte Count; D009504:Neutrophils; D011145:Polyvinyls; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000472:pathogenicity; D051381:Rats; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000472:pathogenicity; D013523:Surgical Equipment
D003470:Culture Media; D011145:Polyvinyls