[Preparation of polytene chromosomes from isolated cell nuclei of the salivary glands of Drosophila].
A method is proposed for preparation of polytene chromosomes free of cytoplasmic substance. The nuclei are isolated using triton X-100 and squashed in 60% propionic acid solution.
D000818:Animals; D002843:Chromatin / Q000032:analysis; D002875:Chromosomes / Q000032:analysis*; D003584:Cytological Techniques; D004331:Drosophila melanogaster; D011092:Polyethylene Glycols; D011422:Propionates; D012469:Salivary Glands / Q000166:cytology*
D002843:Chromatin; D011422:Propionates; D011092:Polyethylene Glycols
Prrparaty politennykh khromosom iz izolirovannykh iader kletok sliunnykh zhelez Drosofili.