Cycloheximide-induced ultrastructural changes in hepatocyte nuclei in partially hepatectomized rats.
Nearly all the chromatin in regenerating rat hepatocytes appears to decondensed form 24 h after partial hepatectomy. When cycloheximide (CXM) is administered to partially hepatectomized rats, a marked condensation of chromatin occurs; 4 h after administration the quantity of condensed chromatin present is much higher than that found in the hepatocytes of sham-operated, untreated rats. No segregation or fragmentation of the nucleolus were, however, observed; this shows that the condensation of chromatin is not by itself sufficient to induce the segregation and fragmentation of the nucleolus. The mechanism governing CMX-induced chromatin condensation in regenerating hepatocytes is discussed.
D000818:Animals; D002466:Cell Nucleolus; D002467:Cell Nucleus / Q000187:drug effects; D002843:Chromatin; D003513:Cycloheximide / Q000494:pharmacology*; D006498:Hepatectomy; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D013997:Time Factors
D002843:Chromatin; D003513:Cycloheximide