The phosphoenolpyruvate : methyl-alpha-D-glucoside phosphotransferase system in Bacillus subtilis Marburg 168 : purification and identification of the phosphocarrier protein (HPr).
The phosphocarrier protein (HPr) of the phosphoenol pyruvate : alpha-methyl-D-glucoside-phosphotransferase system (PTS) has been purified from Bacillus subtilis Marburg 168. The molecular weight is about 8300. HPr contains 1 histidine residue. Phophoenzyme I appears to be an intermediate in the initial phosphoryl transfer from phosphoenolpyruvate (PEP) to HPr. Phospho-HPr is isolated and characterized as a component of the complete system.
D001412:Bacillus subtilis / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification*; D006639:Histidine; D007525:Isoelectric Focusing; D008757:Methylglucosides / Q000378:metabolism; D009097:Multienzyme Complexes; D010446:Peptide Fragments / Q000032:analysis; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010770:Phosphotransferases / Q000302:isolation & purification
D002352:Carrier Proteins; D008757:Methylglucosides; D009097:Multienzyme Complexes; D010446:Peptide Fragments; D006639:Histidine; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases