[Rapid changes in the intensity of fluorescence observed in the presence of cholinergic agonists with Torpedo marmorata membrane fragments rich in cholinergic receptors and labelled with quinacrine].
Stopped-flow studies of receptor-rich membrane fragments from Torpedo marmorata labelled by quinacrine lead to the resolution (in the time scale of the millisecond to the second) of a fast increase of fluorescence intensity associated with the binding of typical agonists. The signal is reversible, does not take place with antagonists, has a Q10 of 1.4 and its amplitude decreases after pretreatment of the membrane fragment by dithiothreitol or ceruleotoxin.
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002217:Carbachol / Q000378:metabolism; D004557:Electric Organ; D005399:Fishes / Q000378:metabolism*; D005453:Fluorescence; D006584:Hexamethonium Compounds / Q000494:pharmacology; D007700:Kinetics; D010277:Parasympathomimetics / Q000378:metabolism*; D011487:Protein Conformation; D011796:Quinacrine; D011950:Receptors, Cholinergic / Q000378:metabolism
D006584:Hexamethonium Compounds; D010277:Parasympathomimetics; D011950:Receptors, Cholinergic; D002217:Carbachol; D011796:Quinacrine; D000109:Acetylcholine
Changements rapides d'intensité de fluorescence observés en présence d'agoniste cholinergique avec des fragments de membranes riches en récepteur cholinergique de Torpedo marmorata marqués avec la quinacrine