Spindle birefringence of isolated mitotic apparatus analysed by pressure treatment.
Sea-urchin zygote mitotic apparatus (MA) isolated in a glycerol/dimethylsulphoxide medium were treated with pressure. Pressure treatment had no effect on spindle birefringence when MA were in full-strength isolation medium. After placing MA in quarter-strength isolation medium, pressures of 4-0 X 10(3)-1-8 X 10(4) lbf in.-2 (2 X 76 X 10(4)-I X 24 X 10(5) k N m-2) for 15 min caused reduction of birefringence which occurred in 2 steps: firstly 20-30% of the birefringence was lost, and then, at higher pressures, the rest of the birefringence was lost. Electron microscopy suggested that pressure-induced changes were in non-microtubule material. Pressure treatment had no effect on MA isolated with hexylene glycol when the MA were pressurized in hexylene glycol; but pressure treatment did cause loss of birefringence when MA isolated in hexylene glycol were transferred immediately into glycerol/dimethylsulphoxide medium and were subsequently treated with pressure (after dilution into quarter-strength glycerol/dimethyl-sulphoxide). We discuss the differences in response between isolated MA and in vivo MA, and we discuss the possibility that 2 components contribute to MA birefringence.
D000818:Animals; D001718:Birefringence; D002458:Cell Fractionation; D002712:Chlorides; D004121:Dimethyl Sulfoxide; D004533:Egtazic Acid; D005260:Female; D005990:Glycerol; D006018:Glycols; D006358:Hot Temperature; D008274:Magnesium; D008870:Microtubules / Q000648:ultrastructure; D008938:Mitosis; D009940:Organoids; D011312:Pressure; D012617:Sea Urchins; D015053:Zygote / Q000648:ultrastructure*
D002712:Chlorides; D006018:Glycols; D004533:Egtazic Acid; D008274:Magnesium; D005990:Glycerol; D004121:Dimethyl Sulfoxide