title: Characterization of polypyrimidines in Drosophila and L-cell DNA.
abstract: Unusually long pyrimidine tracts (polypyrimidines), ranging from 100 to over 1000 nucleotides in length, have been found in Drosophila melanogaster DNA. They are compared to shorter pyrimidine tracts (25-150 nucleotides) which have previously been found in L-cell DNA. Both species were able to anneal to homologous DNA; Drosophila polypyrimidines formed stable hybrids while L-cell polypyrimidines formed hybrids of lower thermal stability. In both cases, the kinetics of the reaction was rapid, suggesting that these tracts are part of highly repeated DNA.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002845:Chromatography; D002850:Chromatography, Gel; D004247:DNA / Q000032:analysis; D004331:Drosophila melanogaster / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006882:Hydroxyapatites; D007739:L Cells / Q000032:analysis*; D051379:Mice; D009693:Nucleic Acid Hybridization; D011119:Polynucleotides / Q000032:analysis*; D013941:Thymine / Q000032:analysis*
chemical_list: D006882:Hydroxyapatites; D011119:Polynucleotides; D004247:DNA; D013941:Thymine