Electroimmunoquantitation of serum proteins cross-linked by glutaraldehyde.
Electroimmunoassay is a simple, rapid and accurate method for quantitating the serum proteins. By use of glutaraldehyde, intermolecular cross-linkage of immunoglobulins to albumin was effected. The conjugation resulted in increased electrophoretic mobility of the immunoglobulins without perceptible change in antigenic determinants. With a Tris-EDTA-boric acid buffer system, the cross-linked immunoglobulins migrated anodically in an electrical field. Six serum proteins from each of 20 samples were quantitated by electroimmunoassay, and the results correlated with values obtained by radial immunodiffusion.
D000447:Aldehydes; D001798:Blood Proteins / Q000032:analysis*; D003176:Complement C3 / Q000032:analysis; D004553:Electric Conductivity; D005976:Glutaral; D006801:Humans; D007118:Immunoassay / Q000379:methods; D005779:Immunodiffusion / Q000379:methods; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D014168:Transferrin / Q000032:analysis; D000515:alpha 1-Antitrypsin / Q000032:analysis
D000447:Aldehydes; D001798:Blood Proteins; D003176:Complement C3; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014168:Transferrin; D000515:alpha 1-Antitrypsin; D005976:Glutaral