Identity of a peritoneal fluid immunoglobulin light chain and the amyloid fibril in primary amyloidosis.
A monoclonal immunoglobulin has been isolated from the peritoneal fluid of a patient with primary amyloidosis. The immunoglobulin was reduced and alkylated and the light and heavy chains were compared to the major protein constituent of that patient's hepatic amyloid fibrils. N-terminal amino acid sequences of the light chain and amyloid fibril were identical when carried to 20 residues and were typical of a kappa I light chain. Molecular weight studies suggested that the fibril protein was composed of an intact light chain with a molecular weight of 23,000. The hypothesis that amyloid fibril protein in primary amyloid is derived from circulating monoclonal immunoglobulin is discussed.
D000595:Amino Acid Sequence; D000682:Amyloid / Q000276:immunology*; D000686:Amyloidosis / Q000276:immunology*; D001202:Ascitic Fluid / Q000276:immunology*; D005260:Female; D006801:Humans; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D008875:Middle Aged
D000682:Amyloid; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M