A membrane filter technique for testing disinfectants.
A membrane filter was used for assessing the surface disinfecting activity of phenolic disinfectants and a chloroxylenol disinfectant. The influence of the type of organism, inoculum size, and hardness of water was investigated. Pseudomonas aeruginosa was chosen for the standardized test. Disinfectant solutions were prepared in water of 300 ppm hardness and applied for two and a half minutes and eight minutes to the bacteria deposited from filtration of 1 ml of a suspension containing 10-6 bacteria. The membrane filter test has certain advantages over many tests, eg, all organisms surviving after treatment can be counted and residual disinfectant is easily removed.
D002452:Cell Count; D002470:Cell Survival; D002713:Chlorine; D003902:Detergents / Q000494:pharmacology / Q000592:standards; D004202:Disinfectants / Q000494:pharmacology / Q000592:standards*; D004926:Escherichia coli / Q000187:drug effects; D005374:Filtration / Q000379:methods*; D007709:Klebsiella / Q000187:drug effects; D008851:Micropore Filters; D010636:Phenols / Q000494:pharmacology / Q000592:standards; D011514:Proteus vulgaris / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013210:Staphylococcus / Q000187:drug effects; D013499:Surface Properties; D014867:Water / Q000032:analysis; D014992:Xylenes / Q000494:pharmacology / Q000592:standards
D003902:Detergents; D004202:Disinfectants; D010636:Phenols; D014992:Xylenes; D014867:Water; D002713:Chlorine