[Formation, by gamma irradiation, of RNA-protein cross-links in E. coli ribosomes].
Gamma irradiation in desaerated conditions of E. coli MRE 600 ribosomes, labelled with C14 uracil, leads to a decrease of extractibility of C14 RNA by lithium chloride 4 M-urea 8 M. On the other hand, the radioactivity of the protein fraction increases with irradiation. These results strongly suggest that RNA-protein cross links are formed in irradiated ribosomes.
D001426:Bacterial Proteins / Q000528:radiation effects; D004926:Escherichia coli; D005720:Gamma Rays; D008970:Molecular Weight; D010100:Oxygen; D012329:RNA, Bacterial / Q000528:radiation effects; D012335:RNA, Ribosomal / Q000528:radiation effects*; D011830:Radiation Effects; D012269:Ribosomal Proteins / Q000528:radiation effects*; D012270:Ribosomes / Q000528:radiation effects*
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D010100:Oxygen
Production, par les rayons gamma, de liaisons ARN-protéines dans les ribosomes d'E. coli