title: Metabolic studies with 65Zn. X. Biosynthesis of alkaline phosphatase in cultures of Pseudomonas fluorescens.
abstract: Alkaline phosphatase from Pseudomonas fluorescens has been partially purified. Labelled 65ZnCl2 in the culture medium is incorporated in the most purified preparations. The enzyme shows a pH optimum of 7.2-7.5 and a Km value for p-nitrophenyl phosphate of 1 times 10(-4). Zinc ions at high concentration inhibit enzyme activity. The protective effect of Mg on the inactivation of alkaline phosphatase by heat is also reported.
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D006358:Hot Temperature; D008274:Magnesium; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D015037:Zinc Radioisotopes / Q000378:metabolism*
chemical_list: D015037:Zinc Radioisotopes; D000469:Alkaline Phosphatase; D008274:Magnesium