title: Improved culture flask for obligate anaerobes.
abstract: An improved flask system for the growth of extremely oxygen-sensitive bacteria in liquid culture is described. The improvement described utilizes an all-glass, neoprene-stoppered flask designed for growth of 50- to 1,000-ml cultures of bacteria with continuous gassing.
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000295:instrumentation*; D003013:Clostridium / Q000254:growth & development; D005069:Evaluation Studies as Topic; D005898:Glass; D008835:Micrococcaceae / Q000254:growth & development; D009387:Neoprene
chemical_list: D009387:Neoprene