Measurement of the staphylococcal epidermolytic toxin: a comparison of bioassay, radial immunodiffusion, and radioimmunoassay.
Three methods of measuring the epidermolytic toxin Staphylococcus aureus-bioassay in newborn mice, radial immunodiffusion, and radioimmunoassay-were compared for reproducibility, specificity, and sensitivity. The bioassay is highly specific and remains the only functional assay. It is reproducible only if newborn mice of the same age are used. The time required for epidermolysis follows a dose-response relationship only if concentrations of toxin large enough to cause peeling in 90 min or less are used. This limits the sensitivity of the bioassay to about 5 mug per ml. Single radial immunodiffusion in agar is a specific and reproducible assay method, but its sensitivity is also about 5 mug per ml. A radioimmunoassay was established by the Farr technique using purified epidermolysin radiolabeled with 125iodine. This assay was highly reproducible and specific. The staphylococcal products, alpha-toxin and enterotoxins A and B, did not cross-react with anti-epidermolysin antibodies. The sensitivity of the radioimmunoassay is 20 ng per ml.
D000818:Animals; D000831:Animals, Newborn; D001681:Biological Assay; D005779:Immunodiffusion; D051379:Mice; D011863:Radioimmunoassay; D012871:Skin Diseases / Q000276:immunology; D013211:Staphylococcus aureus