title: Mapping of mutations affecting synthesis of exocellular enzymes in Bacillus subtilis. Identity of the sacUh, amyB and pap mutations.
abstract: The sacUh, amyB and pap mutations are identical with respect to their pleiotropic phenotype and their genetic location. Strains bearing these mutations overproduce several exocellular enzymes: alpha amylase, lavansucrase and proteases, they are poorly or not at all transformable and most of them are devoid of flagella. These mutations are tightly linked to the sacU- mutations by transformation and therefore lie between the hisA1 and gtaB290 markers. It is possible that the sacUh, amyB and pap mutations on one hand and the sacU- mutations on the other are two different classes of alterations of the same regulatory gene controlling the synthesis of some exocellular enzymes and several other cellular functions. Furthermore an amy- mutation, leading to the lack of alpha-amylase activity, was mapped between the lin2 and aroI906 markers which are not linked to the sacU locus.
mesh_terms: D000681:Amylases / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000201:enzymology*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D005407:Flagella / Q000648:ultrastructure; D005796:Genes; D009154:Mutation; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D010641:Phenotype; D013393:Sucrase / Q000096:biosynthesis*
chemical_list: D000681:Amylases; D013393:Sucrase; D010447:Peptide Hydrolases