Use of the reference method for determination of serum calcium in a quality-assurance survey.
The Reference Method [Clin. Chem. 19, 1208 (1973)] for determination of total calcium in serum has been applied to 29 specimens from the Wellcome Group Quality-Control Programme. The accuracy of the mean results for all participants, and for selected groups of participants, was determined over the concentration range 2.00-2.75 mmol/liter. We show that, in general, continuous-flow (AutoAnalyzer) and atomic absorption methods of analysis have a small, constant bias, but chelation (ethylenediaminetetraacetate) methods are unpredictably inaccurate.
D000818:Animals; D001322:Autoanalysis; D002118:Calcium / Q000097:blood*; D004492:Edetic Acid; D008722:Methods; D011786:Quality Control; D013054:Spectrophotometry, Atomic
D004492:Edetic Acid; D002118:Calcium