Transformation of Bacillus subtilis: transforming ability of deoxyribonucleic acid in lysates of L-forms or protoplasts.
The transformation of Bacillus subtilis by homologous deoxyribonucleic acid (DNA) made available by gently lysing a stable L-form or protoplast suspension was 3 to 10-fold more efficient than DNA isolated by conventional procedures. This increased transformation was not influenced by digestion with pronase, trypsin, or ribonuclease. Preincubation of isolated DNA with L-form lysates did not increase the transformation efficiency above that achieved with untreated, isolated DNA. In addition to displaying a higher efficiency of transformation, the DNA found in these gently prepared lysates was also able to co-transform heretofore unlinked markers at frequencies in excess of those found by congression. Comparison of the frequency of multiple marker transformations to single marker events as a function of DNA dilution conclusively proves that these markers originated from the same continuous strand of DNA.
D001412:Bacillus subtilis / Q000378:metabolism; D001433:Bacteriolysis; D004269:DNA, Bacterial / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D005796:Genes; D007740:L Forms; D009154:Mutation; D011402:Pronase / Q000378:metabolism; D011523:Protoplasts; D012260:Ribonucleases / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014170:Transformation, Genetic; D014316:Tritium; D014357:Trypsin / Q000378:metabolism
D004269:DNA, Bacterial; D014316:Tritium; D003851:Deoxyribonucleases; D012260:Ribonucleases; D014357:Trypsin; D011402:Pronase; D013936:Thymidine