Effect of some inhibitors derived from nitrite on marcomolecular synthesis in Bacillus cereus.
The effect of several inhibitors derived from nitrite on incorporation of [14C]uracil into ribonucleic acid of Bacillus cereus during outgrowth and vegetative growth has been determined. A heat-induced inhibitor presumably of the Perigo type was compared with the nitrosothiols of thioglycolate and beta-mercaptoethanol. All were found to effectively inhibit uracil incorporation at all stages tested. Phase-contrast microscopy revealed that inhibition of morphological events occurred either before germination or during early outgrowth, depending on inhibitor concentration. It was also found that a precursor to the heat-induced inhibitor could be isolated from dialyzable tryptone (a pancreatic digest of casein), which chromatographed as a single species on diethylaminoethyl-cellulose and Sephadex G-25, with an apparent molecular weight of about 2,000.
D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D001409:Bacillus cereus / Q000254:growth & development / Q000378:metabolism*; D006358:Hot Temperature; D008623:Mercaptoethanol; D008970:Molecular Weight; D009573:Nitrites; D009603:Nitroso Compounds / Q000138:chemical synthesis / Q000494:pharmacology; D012329:RNA, Bacterial / Q000096:biosynthesis*; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism; D013864:Thioglycolates; D014498:Uracil / Q000378:metabolism
D000900:Anti-Bacterial Agents; D009573:Nitrites; D009603:Nitroso Compounds; D012329:RNA, Bacterial; D013864:Thioglycolates; D014498:Uracil; D008623:Mercaptoethanol