title: 5-methoxyuridine: a new minor constituent located in the first position of the anticodon of tRNAAla, tRNAThr, and tRNAVal from Bacillus subtilis.
abstract: The sequences of the anticodon of tRNAAla, tRNAThr, and tRNAVal from Bacillus subtilis W 168 were N-G-C, N-G-U, and N-A-C, respectively. A new minor constituent, N, occupied the first position of the anticodon of each tRNA. N was indentified as 5-methoxyuridine (mo5U, Figure 1) by comparison of its UV absorption spectra, Rf values in thin-layer chromatography using several solvent systems and mass spectra with those of chemically synthesized specimen.
mesh_terms: D000409:Alanine; D000926:Anticodon; D001412:Bacillus subtilis / Q000032:analysis*; D001483:Base Sequence; D012329:RNA, Bacterial; D012343:RNA, Transfer; D013912:Threonine; D014529:Uridine / Q000031:analogs & derivatives* / Q000032:analysis; D014633:Valine
chemical_list: D000926:Anticodon; D012329:RNA, Bacterial; D013912:Threonine; D012343:RNA, Transfer; D014633:Valine; D000409:Alanine; D014529:Uridine