A procedure for the quantitative separation of viable and non-viable P815X2 mastocytoma cells.
A method for the separation of viable and non-viable P815X2 mastocytoma cells is described using Ficoll discontinuous density gradients. Non-viable P815X2 cells are more dense than viable P,15X2 cells and reach a bouyant density equilibrium at a higher Ficoll concentration than the viable cells. This technique allows the separation of viable P815X2 cells from cells that had been killed by antiserum and complement, or by non-growth supporting medium or by cytotoxic lymphocytes. This method is a new assay to determine cytotoxic lymphocyte killing of P815X2 cells.
D000818:Animals; D002469:Cell Separation; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D003165:Complement System Proteins / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D007106:Immune Sera; D008297:Male; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011817:Rabbits; D013154:Spleen / Q000276:immunology
D007106:Immune Sera; D003165:Complement System Proteins