[Incorporation of 3H-5 uridine and 3H-5 leucine into cultured planarian cells, Polycelis tenuis (Iijima) in vitro].
RNA and protein synthesis in Planarian cells cultivated in vitro was studied by histoautoradiography. In the non-differentiated cells, uptake of precursor is intense from the beginning of the culture, and sensitive to addition of trophic factor known for their activating effect on mitosis and regeneration. On the contrary the rate of incorporation in differentiated cells is low and uniform, independently of the differents factors added to the medium.
D001345:Autoradiography; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006257:Head; D007930:Leucine / Q000378:metabolism; D010932:Planarians / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012038:Regeneration / Q000187:drug effects; D014419:Turbellaria / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism
D012313:RNA; D007930:Leucine; D014529:Uridine
Incorporation d'uridine 3H-5 et de leucine 3H-5 dans les cellules de Planaires Polycelis tenuis (Iijima) cultivées in vitro