Quantitative determination of antigens in biological fluids by the antibody radial immunodiffusion method. Antigens in agar gel.
An antibody diffusion method is described for quantitative determination of antigens in biological fluids. It is a multiple radial immunodiffusion (MRD) method. The fluid is mixed with agar gel during the test and poured on to a plate of standard size and antisera are placed in circular reservoirs. On the same plate as many proteins can be determined as there are monospecific antisera available. The calibration curve is made by means of standard antigen solutions (normal human sera). There is no need to concentrate even biological fluids of low protein concentration (cerebrospinal fluid, urine etc.). The implementation of the process for various body fluids, as well as the advantages and disadvantages of the method are described.
D000362:Agar; D000941:Antigens / Q000032:analysis*; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D008433:Mathematics
D000941:Antigens; D000362:Agar