Amino acid sequence of a vitamin K-dependent Ca2+-binding peptide from bovine prothrombin.
The amino acid sequence of a 31-residue peptide from bovine prothrombin has been determined. This peptide has been shown to contain the vitamin K-dependent modification required for Ca2+ binding (Nelsestuen, G. L., and Suttie, J. W. (1973) Proc. Natl. Acad. Sci. U. S. A. 70, 3366-3370) and the modified amino acid, gamma-carboxyglutamic acid (Nelsestuen, G. L., Zytkovicz, T., and Howard, J. B. (1974) J. Biol. Chem. 249, 6347-6350). The peptide was shown to correspond to residues 12 to 42 of prothrombin.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002268:Carboxypeptidases; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D007531:Isoflurophate; D010434:Pepsin A; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism*; D011402:Pronase; D011516:Prothrombin / Q000378:metabolism; D011955:Receptors, Drug; D013305:Streptomyces griseus / Q000201:enzymology; D013820:Thermolysin; D014357:Trypsin; D014812:Vitamin K / Q000378:metabolism*
D000596:Amino Acids; D010446:Peptide Fragments; D011955:Receptors, Drug; D014812:Vitamin K; D007531:Isoflurophate; D011516:Prothrombin; D002268:Carboxypeptidases; D014357:Trypsin; D010434:Pepsin A; D011402:Pronase; D013820:Thermolysin