[Ribosomal apparatus of liver cells in the process of carinogenesis induced by 4-dimethylaminoazobenzene].
A content of membrane-bound and free ribosomes, sedimentation distribution of total polyribosomes in a linear sucrose density gradient and their functional activity in a cell-free system of protein synthesis were estimated in liver of animals feeded with protein-poor rice diet containing a cancerogen, 4-dimethylaminoazobenzene (DAB) at a dose of 15 mg per day. Periods of the experiments were 1-10 days, 1-2, 3-4 and 5 months from the beginning of DAB treatment. The decrease of protein-synthesizing activity, the decrease in the content of membrane-bound ribosomes and the oncrease in the content of free ribosomes in animal liver were observed at first days of DAB action. Changes in the quantitative ratio between membrane-bound and free ribosomes (in the direction of decreasing the content of membrane-bound ribosomes) was observed at all the stages of DAB-cancerogenesis. It is supposed that the uncoupling of ribosome-membrane bonds in endoplasmic reticulum takes an important part in cancerogenesis, disturbing intracellular regulation of protein synthesis.
D000284:Administration, Oral; D000818:Animals; D002458:Cell Fractionation; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002474:Cell-Free System / Q000502:physiology; D002499:Centrifugation, Density Gradient; D003584:Cytological Techniques; D004032:Diet; D004721:Endoplasmic Reticulum / Q000187:drug effects; D008099:Liver / Q000166:cytology*; D008297:Male; D011132:Polyribosomes / Q000032:analysis / Q000502:physiology; D051381:Rats; D012270:Ribosomes / Q000032:analysis / Q000187:drug effects*; D013997:Time Factors; D004124:p-Dimethylaminoazobenzene
D004124:p-Dimethylaminoazobenzene
Ribosomnyĭ apparat kletok pecheni v protsesse kantserogeneza, indutsirovannogo 4-dimetilaminoazobenzolom