Isolation of rat and rabbit IgM from normal serum using anti-human mu antibody-polyacrylamide beads immunosorbents.
The antigenic relationship between human IgM and IgM from other species has been utilized in isolating rat and rabbit IgM from normal sera by means of an anti-human mu-polyacrylamide beads immunoadsorbent. The isolated IgM was found to be pure by immunoelectrophoretic analysis and Ouchterlony technique. The antisera obtained were monospecific for mu-chains after absorption with IgG. Lastly, the IgM isolated always retained its antibody activity.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M / Q000302:isolation & purification*; D007148:Immunoglobulin mu-Chains; D008297:Male; D011817:Rabbits; D051381:Rats
D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains