Methionine degradation by Pseudomonas fluorescens UK1 and its methionine-utilizing mutant.
Pseudomonas fluorescens UK1, and a mutant derived from it that can use methionine as its sole source of carbon and nitrogen, were used to study methionine degradation. Radioactive 2-oxomethionine, 2-oxobutyric acid and carbon dioxide comprised 95% of [I-14C]methionine consumed by the mutant. Demethiolating activity was detected in both strains when they were grown with methionine. The ability to grow on methionine depended on the decarboxylation of 2-oxobutyric acid derived from it. This decarboxylating activity was lost if the growth media contained an additional carbon source. No evidence was obtained for the operation of the cystathionine pathway.
D002087:Butyrates / Q000378:metabolism; D002245:Carbon Dioxide / Q000096:biosynthesis; D003653:Decarboxylation; D004926:Escherichia coli / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D008715:Methionine / Q000031:analogs & derivatives / Q000378:metabolism*; D009154:Mutation; D011551:Pseudomonas fluorescens / Q000254:growth & development / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D002087:Butyrates; D005971:Glutamates; D002245:Carbon Dioxide; D008715:Methionine; D005947:Glucose