title: Isolation and properties of the DNA of African swine fever (ASF) virus.
abstract: African swine fever (ASF) virus was grown either in swine macrophages or in VERO cells and purified free of cell DNA. Virus DNA was isolated from virions as a molecule with a sedimentation coefficient of 60S and a contour of 58 +/- 3 mum. .these two values give a mol. wt. of 102 +/- 5 X 10(6) and 107 +/- 5 X 10(6), respectively, for the genome of ASF virus. Denatured DNA fragments from ASF virus reassociate with a C0t1/2 value of 0-60 +/- 0-05 MS, which compared with the corresponding value for T4 DNA gives for the molecular mass of ASF virus DNA a value of 102 +/- 8 X 10(6) daltons. Only virus DNA is synthesized ASF virus-infected swine macrophages.
mesh_terms: D000358:African Swine Fever Virus / Q000032:analysis*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004267:DNA Viruses / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D000882:Haplorhini; D008264:Macrophages; D008970:Molecular Weight; D009695:Nucleic Acid Renaturation; D013552:Swine
chemical_list: D004279:DNA, Viral