Electrophoretic separation of Bacillus subtilis genes.
The cleavage of Bacillus subtilis DNA by EcoR1 restriction endonuclease produced segments which retain various degrees of genetic transforming activity. The active segments analyzed thus far, range in size from 23 to 3 kilobases and can be partially separated by agarose gel electrophoresis. Various markers can thus be enriched from 30- to 60-fold.
D001412:Bacillus subtilis / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D003201:Computers; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005796:Genes; D008040:Genetic Linkage; D005838:Genotype; D008722:Methods; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes