USe of tissue culture medium for in vitro studies on the ion transport capacity of amphibian epithelia.
The sodium transport capacity across frog skin and toad bladder in vitro is enhanced when a tissue culture medium is used to mount the preparations, instead of the classic Ringer solution. The response to antidiuretic hormone used 24 h after isolating the tissues is also higher in preparations incubated in the same culture medium.
D000818:Animals; D001001:Anura; D001693:Biological Transport, Active; D002023:Bufo bufo; D003470:Culture Media; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials; D011898:Ranidae; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*; D014667:Vasopressins / Q000494:pharmacology
D003470:Culture Media; D014667:Vasopressins; D012964:Sodium