Dispersion of epinephrine sensitive and insensitive adenylate cyclase from the ciliate Tetrahymena pyriformis.
The membrane bound adenylate cyclase (ATP pyrophosphate-lyase (cyclizing) EC 4.6.1.1) of the ciliate Tetrahymena pyriformis could be extracted by washing the membrane fraction with 0.25 M sucrose. The enzyme dissociated in this way did not sediment after centrifugation at 105 000 times g for 2 h and was still responsive to stimulation by epinephrine. Dispersion of the membranes with Triton-X 100 led to purified preparation of the cyclase, which was no longer stimulated by epinephrine but retained fully the activation by fluoride and serotonin.
D000262:Adenylyl Cyclases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D002848:Chromatography, DEAE-Cellulose; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology*; D005459:Fluorides / Q000494:pharmacology; D011092:Polyethylene Glycols; D012701:Serotonin / Q000494:pharmacology; D013347:Subcellular Fractions / Q000201:enzymology; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000201:enzymology*
D012701:Serotonin; D011092:Polyethylene Glycols; D000262:Adenylyl Cyclases; D005459:Fluorides; D004837:Epinephrine