[The effect of nitrogen mustard on the incorporation of tritiated thymidine into chick embryo limb buds. Autoradiographic study].
Tritiated thymidine labelling has been performed during the 24 hours following the treatment of chick embryos with nitrogen mustard. The rate of incorporation into nuclei of control and treated limb buds varied with the stage of the embryo. In the case of the nitrogen mustard-treated limb buds, a marked inhibition of the incorporation has been observed in the mesenchyme and the apical cap. However, 18 and 24 hours after treatment, the rate of labelling in the apical cap was the same as that in the control.
D000014:Abnormalities, Drug-Induced / Q000196:embryology*; D000367:Age Factors; D000818:Animals; D002642:Chick Embryo; D004261:DNA Replication / Q000187:drug effects*; D003864:Depression, Chemical; D004475:Ectoderm / Q000378:metabolism; D005121:Extremities / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D008466:Mechlorethamine / Q000494:pharmacology*; D008648:Mesoderm / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D008466:Mechlorethamine; D013936:Thymidine
Action de l'ypérite azotée l'incorporation de thymidine tritiée dans les bourgeons de membres de l'embryon de poulet. Etude histoautoradiographique