[Specific immune response and immunoglobulin production in intestinal infections. II. Methodological aspects of studying immunoglobulins in digestive tract secretions].
Application of radial immunodiffusion for the study of immunoglobulins in the digestive tract secretion is described. Sensitivity of the method is increased by high dilution of antisera; precipitation rings should be recorded in the stained plates. To quantitate both serum and secretory IgA it is suggested that the secretions should be studied in parallel with two antisera--against the alpha-chain (to determine the total IgA) and against SC (to determine the secretory IgA). It is recommended to calculate the amount of immunoglobulins per 1 mg of high-molecular protein precipitable by trichloracetic acid.
D004405:Dysentery, Bacillary / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis*; D007419:Intestinal Secretions / Q000276:immunology*
D007070:Immunoglobulin A
Spetsificheskiĭ immunnyĭ otvet i produktsiia immunoglobulinov pri kishechnykh infektsiiakh. Soobshchenie II. Metodicheskie osobennosti issledovaniia immunoglobulinov v sekretakh pishchevaritel'nogo trakta