Crystallization and preliminary x-ray investigation of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens.
P-Hydroxybenzoate hydroxylase (EC 1.14.13.2) can be crystallized from 0.1 M potassium phosphate buffer, pH 7.5, 39% saturated ammonium sulfate, and 1mM p-hydroxybenzoate. The space group is C2221 with 8 molecules/unit cell. When p-hydroxybenzoate is removed, the diffraction pattern and the cell dimensions change.
D015109:4-Hydroxybenzoate-3-Monooxygenase; D003460:Crystallization; D006899:Mixed Function Oxygenases; D011551:Pseudomonas fluorescens / Q000201:enzymology; D014961:X-Ray Diffraction
D006899:Mixed Function Oxygenases; D015109:4-Hydroxybenzoate-3-Monooxygenase