Activation of the alternative (properdin) pathway by divalent cations.
Mg++, Mn++ and Co++ activate the C system as judged by the reduction in cobra venom factor indeed hemolysis, and cleavage of properdin factor B and C3. The maximum effect requires the addition of 5 to 10 mM of these cations to dialyzed sera respond in similar fashion indicating that these divalent cations can trigger the alternative pathway without the addition other activating agents.
D000818:Animals; D001779:Blood Coagulation Factors / Q000302:isolation & purification; D002118:Calcium; D002266:Carboxymethylcellulose Sodium; D002413:Cations, Divalent / Q000494:pharmacology*; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D003035:Cobalt / Q000494:pharmacology; D003176:Complement C3 / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism*; D003911:Dextrans; D004492:Edetic Acid; D004533:Egtazic Acid; D006168:Guinea Pigs; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007106:Immune Sera; D007122:Immunoelectrophoresis; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D011414:Properdin / Q000378:metabolism*; D011817:Rabbits / Q000276:immunology; D051381:Rats; D012712:Serum Globulins / Q000032:analysis; D012911:Snakes; D014688:Venoms
D001779:Blood Coagulation Factors; D002413:Cations, Divalent; D003176:Complement C3; D003911:Dextrans; D007106:Immune Sera; D012712:Serum Globulins; D014688:Venoms; D011414:Properdin; D003035:Cobalt; D008345:Manganese; D004533:Egtazic Acid; D003165:Complement System Proteins; D004492:Edetic Acid; D008274:Magnesium; D002266:Carboxymethylcellulose Sodium; D002118:Calcium