Contributions to the perfecting of the enzymatic method used for the determination of ATP in biological systems.
The improvement of methods for the determination of ATP, that is the increasing of their sensitivity is extremely useful for the study of excitation-energy coupling in excitable membranes. Such investigations from the object of the present work in which there is proved that the microanalytical method for the dosing of ATP, based on the enzymatic reaction with luciferin-luciferase system, may be converted into a ultramicroanalytical method and therefore used also in other fields of research, as for example the ATP biosynthesis in cell cultures.
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D001001:Anura; D049454:Firefly Luciferin; D008156:Luciferases; D008722:Methods; D008832:Microchemistry; D012584:Sciatic Nerve / Q000032:analysis
D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D008156:Luciferases