Heavy chain-producing variants of a mouse myeloma cell line.
Eleven variants producing only heavy (H) chains have been isolated from the P3 cell line after mutagenesis with either ICR-191 or nitrosoguanidine. The H chains produced by these variants are similar both in molecular weight and by peptide maps with the parental H chain. H chains dimerize intreacellularly, are not secreted but persist as stable cytoplasmic proteins. No evidence for the continued synthesis of light (L) chains of L chain fragments has been found in these variants. These studies show that, in the P3 cell line, the synthesis of H chains can continue in the absence of detectable L chain synthesis.
D000166:Acridines; D000818:Animals; D002460:Cell Line; D014644:Genetic Variation; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D009101:Multiple Myeloma / Q000276:immunology*; D009153:Mutagens; D009154:Mutation; D009194:Myeloma Proteins / Q000096:biosynthesis; D009604:Nitrosoguanidines; D010455:Peptides / Q000032:analysis
D000166:Acridines; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D009153:Mutagens; D009194:Myeloma Proteins; D009604:Nitrosoguanidines; D010455:Peptides