The mode of action of ASK-753 on Bacillus subtilis.
The mode of action of ASK-753 on Bacillus subtilis was examined. Unlike proper sideromycin antibiotics ferrioxamine B failed to antagonize the antimicrobial effects of ASK-753. The antibiotic could inhibit the biosynthesis of nucleic acids; effect on the RNA was more pronounced. ASK-753 affected the stability of prelabelled DNA of B, subtilis in growing or resting cultures; the effect on the latter was more pronounced. Lysis of B, subtilis protoplasts could be attained at 30 degrees C but not at 4 degrees C which excludes a possible detergent affect of the drug. The drug exerted a potent inhibiting influence on protein synthesis by arresting the activity of lysyl-tRNA synthetase and thus could prevent the incorporation of 14C-lysine.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000096:biosynthesis; D005290:Ferric Compounds / Q000494:pharmacology; D007501:Iron; D010455:Peptides / Q000494:pharmacology*; D011523:Protoplasts / Q000187:drug effects; D012329:RNA, Bacterial / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005290:Ferric Compounds; D010455:Peptides; D012329:RNA, Bacterial; D007501:Iron