[Conjugational transfer of R factor RP1 in Ps. aeruginosa and the possibility of inhibiting this process with a series of substances].
It was shown that the conjugation system of Ps. aeruginosa PAO 2604 X PTO 629 rifr used for transfer of the plasmid markers of R-factor RPI was a convenient model for a number of genetic investigations (the rate of transfer of the antibiotic resistance markers was 2.2 X 10(-3) to 8.8 X 10(-5). 80.7 per cent of the exconjugants obtained from this crossing acquired all 3 resistance plasmid markers (carbenicillin tetracycline, neomycin). In 12 per cent of R+-exconjugants transfer of 2 or 1 resistance determinant of R-factor was observed. The use of the above conjugation system revealed the inhibitory effect of bonafton (an antiviral drug), acridine dyes (acrichin, metachrome orange), ethidium bromide and rifampicin. A possibility of intraspecies transfer of resistance plasmid markers was found in crosses Ps. aeruginosa PAO 2604 X X E. coli CSH--2 rifr and Ps. aeruginosa PAO 2604 X E. coli C600 rifr. Transfer of the resistance markers was observed in combinations of carbenicillin, tetracycline, neomycin (Cb, Tc, Nm), tetracycline (Tc) and carbenicillin (Cb).
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology; D002228:Carbenicillin / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000187:drug effects; D003227:Conjugation, Genetic / Q000187:drug effects*; D003470:Culture Media; D008670:Metals / Q000494:pharmacology; D009355:Neomycin / Q000494:pharmacology; D010403:Penicillin Resistance / Q000187:drug effects*; D010957:Plasmids / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D011815:R Factors / Q000187:drug effects*; D012293:Rifampin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D003470:Culture Media; D008670:Metals; D013752:Tetracycline; D002228:Carbenicillin; D009355:Neomycin; D012293:Rifampin; D013307:Streptomycin
Kon"iugatsionnaia peredacha R-faktora RP1 u Ps. aeruginosa i vozmozhnost' ingibirovaniia étogo protsessa riadom veshchestv