Quantitative analysis of individual bile acids by gas-liquid chromatography: an improved method.
The quantitative analysis of individual bile acids by gas-liquid chromatography has been improved by column oven temperature programming and by a new liquid phase, SP-2401. The method is fast; bile acids are well resolved; retention times are reproducible; detector responses are linear and sensitive to 0.1 mug: and there is little adsorption onto the liquid phase. The method has been successfully used for bile, and it has the potential for use on serum.
D000818:Animals; D001646:Bile / Q000032:analysis*; D001647:Bile Acids and Salts / Q000032:analysis*; D002635:Chenodeoxycholic Acid / Q000032:analysis; D002642:Chick Embryo; D002793:Cholic Acids / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods; D003840:Deoxycholic Acid / Q000032:analysis; D005069:Evaluation Studies as Topic; D000882:Haplorhini; D008095:Lithocholic Acid / Q000032:analysis; D012453:Saimiri; D014269:Trifluoroacetic Acid
D001647:Bile Acids and Salts; D002793:Cholic Acids; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D008095:Lithocholic Acid; D014269:Trifluoroacetic Acid