Radioimmunoassay of main urinary metabolite of prostaglandin F2alpha.
Radioimmunoassay of 5alpha, 7alpha-dihydroxy-11-keto-tetranorprosta-1,16-dioic acid, main urinary metabolite of prostaglandin F2alpha (PGF2alpha), was performed using an antiserum produced in the rabbit. The antibody in 100 mu1 of 1,600-fold diluted antiserum binds with 60 picograms of metabolite. The main urinary metabolite level fell when flufenamic acid, a prostaglandin synthetase inhibitor, was given to rats. In contrast, it was significantly elevated when PGF2alpha was administered.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D003429:Cross Reactions; D016861:Cyclooxygenase Inhibitors; D003864:Depression, Chemical; D005439:Flufenamic Acid / Q000494:pharmacology; D007106:Immune Sera; D007213:Indomethacin / Q000494:pharmacology; D011460:Prostaglandins F / Q000652:urine*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D013997:Time Factors
D016861:Cyclooxygenase Inhibitors; D007106:Immune Sera; D011460:Prostaglandins F; D005439:Flufenamic Acid; D001241:Aspirin; D007213:Indomethacin