Histochemical demonstration of the effect of partial hepatectomy on the rat liver enzyme activity of 3 alpha-, 17beta-hydroxisteroid dehydrogenase and glucose-6-phosphate dehydrogenase.
The dynamics of the partial hepatectomy effect on the activity of 3alpha-HSD, 17beta-HSD and G-6-PD in rat liver has been followed by means of histochemical methods. In all stages of the investigation the 3alpha-HSD and G-6-PD did not show any deviation from the normal histologic findings in the sham-operated control animals. The reaction for 17beta-HSD decreased abruptly and more or less approximately evenly in the liver cells of the hepatectomized animals as early as in the 24th h after operation. That went on until the 48th h. During the 72nd, 96th and 120th h a certain increase of the 17beta-HSD activity was established. On the 8th day the intensity of the histochemical reaction was identic with that in the liver cells of control animals. The histochemical changes of the 17beta-HSD enzyme activity coincide fully in time, strength and direction with the rate of liver regeneration. During the first 2 days after partial hepatectomy, during the rapid mitotic division and loss of the highly specialized function of the hepatic cells, the activity of the 17beta-HSD enzyme greatly decreases, probably in connection with the involvement of cell proteins in the synthesis of new structures. When the regeneration rate diminishes, about the 5th to 8th day, the liver cells gradually return to a quiet ("differentiated") state, regenerate their enzyme systems (17beta-HSD) and renew the normal function of metabolizing steroid hormones.
D000818:Animals; D002454:Cell Differentiation; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006498:Hepatectomy; D006651:Histocytochemistry; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D013997:Time Factors
D006913:Hydroxysteroid Dehydrogenases; D005954:Glucosephosphate Dehydrogenase