Isolation of the soluble substrate recognition component of the dicarboxylate transport system of Escherichia coli.
A soluble, periplasmic protein was isolated from Escherichia coli cells by chromatography on columns of aspartate-coupled Sepharose 4B. This protein has a molecular weight of about 15,000 and, as judged by competition experiments, binds the three dicarboxylic acids, succinate, malate, and fumarate and, addition, the monocarboxylic acid D-lactate. The periplasmic protein seems to be missing from some mutants of E. coli (designated cbt) which are incapable of transporting succinate in whole cells.
D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D001693:Biological Transport, Active; D002264:Carboxylic Acids / Q000494:pharmacology; D002846:Chromatography, Affinity; D003998:Dicarboxylic Acids / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008293:Malates / Q000378:metabolism; D008970:Molecular Weight; D011955:Receptors, Drug; D012995:Solubility; D013329:Structure-Activity Relationship; D013386:Succinates / Q000378:metabolism; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D001426:Bacterial Proteins; D002264:Carboxylic Acids; D003998:Dicarboxylic Acids; D005650:Fumarates; D007773:Lactates; D008293:Malates; D011955:Receptors, Drug; D013386:Succinates; D013439:Sulfhydryl Reagents; D004492:Edetic Acid; D008274:Magnesium; D015032:Zinc