[Interaction of various acceptors with oxygen anion-radicals in liver microsomes].
Interaction of acceptors with oxygen (O2) anion-radicals appearing in the microsomal chain of electron transfers, was studied. It was found that nitro blue tetrazolium (NBT) was reduced in liver microsomes both in the presence of O2 and through direct acceptance of electrons. The values of Km and Vmax for NBT and epinephrine interactions with microsomes were determined. The rate of O2 generation was calculated from the data obtained.
D000818:Animals; D004579:Electron Transport; D004837:Epinephrine / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D009246:NADH Tetrazolium Reductase / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010101:Oxygen Consumption; D011808:Quinone Reductases / Q000378:metabolism; D051381:Rats
D009580:Nitroblue Tetrazolium; D010088:Oxidoreductases; D009246:NADH Tetrazolium Reductase; D009251:NADPH-Ferrihemoprotein Reductase; D011808:Quinone Reductases; D004837:Epinephrine
Vzaimodeĭstvie razlichnykh aktseptorov s anion-radikalami kisloroda v mikrosomakh pecheni