title: The origin and characteristics of a pig kidney cell strain, LLC-PK.
abstract: A stable epithelial-like pig kidney cell strain has been established. This strain has been carried through more than 300 serial passages, has remained free of microbial and viral contaminants, and has retained a near diploid number of chromosomes. Attempts to produce tumors with these cells in immunosuppressed laboratory animals have been uniformly negative. The cells have grown rapidly in monolayer cultures with a split ratio of 1 to 15 at weekly intervals, but have failed to proliferate in suspension cultures. A subline adapted to growth on serum-free medium 199 has been carried through 145 passages on this medium. Several unusual morphologic features have been observed in these cultures including three-dimensional "domelike" structures. These cells have been found susceptible to some viruses and have been especially useful for viruses of domestic animals. LLC-PK1 cells have produced significant levels of plasminogen activator.
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D004171:Diploidy; D005615:Freezing; D007165:Immunosuppression Therapy; D007668:Kidney; D008297:Male; D009374:Neoplasms, Experimental / Q000209:etiology; D010960:Plasminogen Activators / Q000096:biosynthesis; D011309:Preservation, Biological; D051381:Rats; D013552:Swine; D014776:Virus Cultivation; D014780:Viruses / Q000254:growth & development
chemical_list: D010960:Plasminogen Activators