Localization of J-chain and interchain disulfide bonds in a human F(c)5mu-like fragment.
The inter H-H cysteinyl peptides and the localization of the J-chain were studied in a human F(c)5mu-like fragment. The latter was found to be built up by non-covalent association of molecular forms of 140 000, 95 000 and 70 000 dalton subunits. The trimeric, dimeric and monomeric forms were obtained from gradual reduction by dithiothreitol of the major component of 140 000 daltons, thus confirming the tetrameric nature of this subunit. The latter was found to result from the association of both components of the 70 000 dalton subunit, with the participation of the inter H-H subunit bridge. Structural analysis of the labelled peptides obtained by partial reduction and alkylation showed the presence of the intersubunit disulfide bridge and of the inter heavy-heavy chain bridge of the C-terminal region, and the absence of the heavy-heavy chain bridge of the hinge region. The sequence of these peptides is identical to the sequences of the corresponding peptides of normal mmu-chains. The J-chain, which was covalently linked to this F(c)5mu-like fragment, was found to be predominantly associated within the 95 000 dalton subunit. The results showed that the J-chain was linked in the protein as a "clasp" within a single subunit and not between two subunits.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D004220:Disulfides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007141:Immunoglobulin Fc Fragments; D007144:Immunoglobulin J-Chains; D046911:Macromolecular Substances; D008970:Molecular Weight; D010434:Pepsin A; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D011694:Purpura, Hyperglobulinemic / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D014357:Trypsin
D000596:Amino Acids; D004220:Disulfides; D007141:Immunoglobulin Fc Fragments; D007144:Immunoglobulin J-Chains; D046911:Macromolecular Substances; D010446:Peptide Fragments; D014357:Trypsin; D010434:Pepsin A