The action of hydrogen peroxide on the hydroxylation of p-coumaric acid by spinach-beet phenolase.
Treatment of spinach-beet phenolase with H2O2 under aerobic conditions results in a stimulation of the p-coumaric acid hydroxylation it catalyses, but not the caffeic acid oxidation. Spectroscopic evidence suggests that an oxygenated enzyme species is formed under these conditions.
D002109:Caffeic Acids / Q000032:analysis; D004156:Catechol Oxidase / Q000378:metabolism*; D002854:Chromatography, Paper; D002934:Cinnamates / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006900:Hydroxylation; D010944:Plants / Q000201:enzymology; D013053:Spectrophotometry
D002109:Caffeic Acids; D002934:Cinnamates; D003373:Coumaric Acids; D006861:Hydrogen Peroxide; D004156:Catechol Oxidase