Purification of polyribosomes, 70S, monomers or ribosomal subunits by gel filtration.
Chromatography through Bio-Gel columns is a simple and rapid method for the purification of different ribosomal particles. This mild procedure, easily adapted to many purposes with good yields, can substitute the conventional sucrose gradient centrifugations, especially when the integrity of labile particles or complexes must be preserved.
D002458:Cell Fractionation; D002850:Chromatography, Gel / Q000379:methods; D004926:Escherichia coli / Q000648:ultrastructure; D011132:Polyribosomes / Q000032:analysis*