Preparation of protoplasts of group H streptococci (Streptococcus sanguis).
Stable protoplasts of several strains of group H streptococci (Streptococcus sanguis) can be prepared by use of group C streptococcal phage-associated lysin in the presence of 30% raffinose. Sucrose cannot be substituted for raffinose. Protoplast formation did not require the addition of Mg2+; however, this cation enhanced their stability. Some other strains, also presumptive group H streptococci, were not sensitive to phage-associated lysin.
D001433:Bacteriolysis; D001435:Bacteriophages / Q000276:immunology; D002462:Cell Membrane / Q000648:ultrastructure; D002473:Cell Wall / Q000648:ultrastructure; D004492:Edetic Acid / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D009996:Osmotic Fragility / Q000187:drug effects; D011523:Protoplasts / Q000187:drug effects / Q000648:ultrastructure; D013291:Streptococcus / Q000187:drug effects / Q000648:ultrastructure; D013395:Sucrose / Q000494:pharmacology; D014312:Trisaccharides / Q000494:pharmacology
D014312:Trisaccharides; D013395:Sucrose; D004492:Edetic Acid; D008274:Magnesium