title: The comparison of rat liver rough endoplasmic reticulum membrane proteins before and after in vitro removal of its bound ribosomes.
abstract: The comparison of the proteins of rat liver rough membrane after stripping with EDTA or KCl-puromycin by two dimensional gel electrophoresis is described. By stripping the membrane with EDTA, most of the basic ribosomal proteins are still attached to the membrane; in contrast to the EDTA stripping method, treatment with KCl-puromycin removes most of the ribosomal proteins and does not remove any of the membranal proteins.
mesh_terms: D000818:Animals; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008566:Membranes / Q000032:analysis; D011132:Polyribosomes / Q000032:analysis; D011189:Potassium Chloride; D011691:Puromycin; D051381:Rats; D012269:Ribosomal Proteins / Q000032:analysis*; D012270:Ribosomes / Q000032:analysis*; D012995:Solubility
chemical_list: D012269:Ribosomal Proteins; D011691:Puromycin; D011189:Potassium Chloride; D004492:Edetic Acid