Transcription from complementary deoxyribonucleic acid strands in various sporogenic and asporogenic mutants of Bacillus subtilis. Hybridization-competition studies on ribonucleic acid synthesized in vivo by a thermosensitive sporulation mutant (ts-4).
The present paper describes an investigation, at the transcription level, in a thermosensitive sporulation mutant of Bacillus subtilis (ts-4) grown at the permissive (30 degrees C) or restrictive (42 degrees C) temperature where sporulation capacity is respectively expressed or arrested. These studies were carried out by analysing the ribonucleic acid from vegetative and stationary phase cells (t3 cells) grown under both conditions, by hybridization-competition experiments.
D001412:Bacillus subtilis / Q000378:metabolism*; D001667:Binding, Competitive; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000378:metabolism*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000096:biosynthesis*; D013171:Spores, Bacterial / Q000378:metabolism; D013696:Temperature; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012329:RNA, Bacterial