Control of J chain biosynthesis in relation to heavy and light chain synthesis, polymerization and secretion.
Mouse myeloma tumors and some variants derived from them were labeled in vitro with tritiated leucine and the radioactive J chain was assayed in cell lysates by precipitation with an antiserum specific for mouse J chain. The major findings were: 1) J chain can be found in an IgG2b-secreting cells (MPC-11). These data, together with previous findings suggest that cells secreting all classes of IgG synthesize J chain, even though there is no apparent requirement for J chain in assembly of the IgG molecule. Hence production of J chain does not depend upon secretion of a polymeric immunoglobulin. 2) Intracellular J chain can be found in myeloma variants that do not produce heavy chains showing that production of J chain may not coordinately be linked to the synthesis of heavy chain. 3) J chain was found in cells synthesizing, but not secreting, immunoglobulin. Thus production of J chain is not linked to secretion of immunoglobulin. 4) J chain could not be detected in plasma cells that do not produce immunoglobulins. It was also not found in mouse leukemic cells, suggesting that production of J chain is probably linked in some way to immunoglobulin production.
D000818:Animals; D000917:Antibody Formation; D002460:Cell Line; D002478:Cells, Cultured; D006041:Goats / Q000276:immunology; D007106:Immune Sera; D007070:Immunoglobulin A / Q000096:biosynthesis; D007128:Immunoglobulin Fragments / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis*; D007144:Immunoglobulin J-Chains / Q000096:biosynthesis*; D007075:Immunoglobulin M; D007930:Leucine / Q000378:metabolism; D007942:Leukemia, Experimental / Q000276:immunology; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology; D011108:Polymers; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D014316:Tritium
D007106:Immune Sera; D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007144:Immunoglobulin J-Chains; D007075:Immunoglobulin M; D011108:Polymers; D014316:Tritium; D007930:Leucine