Gas-chromatographic/mass-spectrometric determination of theophylline in whole blood.
We describe a combined gas-chromatographic mass-spectrometric method for determination of theophylline in whole blood. With use of a probability-based matching approach, the extensive gas-chromatographic interference did not affect mass-spectrometric quantitation of theophylline. Concentrations expected and found were linearly related from 1 to 60 mg/liter; the run-to-run coefficient of variation was less than 8%, within-run less than 6%. Some other xan-thines and drugs frequently co-administered with theophylline did not affect results. Only 0.2 ml of blood is required, which makes the procedure useful for monitoring theophylline in newborns and pediatric populations.
D002849:Chromatography, Gas / Q000379:methods; D005069:Evaluation Studies as Topic; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008832:Microchemistry; D013806:Theophylline / Q000097:blood*
D013806:Theophylline