The aromatization of androgens by primary monolayer cultures of fetal rat hypothalamus.
Aromatization of [3H]androstenedione and [3H]19-hydroxyandrostenedione to [3H]estrone has been demonstrated to occur in one to two week old primary monolayer cultures of fetal rat hypothalamus. Three times more estrone is produced from 19-hydroxyandrostenedione than from androstenedione in four day incubations. Cultures treated with cytosine arabinoside have 50% less cellular protein and produce three times more estrone from either substrate than untreated cultures. Time course experiments using cultures treated with cytosine arabinoside indicate that aromatization of 19-hydroxyandrostenedione is linear for three days and can be quantitatively measured within the first two to eight hours of incubation.
D000735:Androstenedione / Q000031:analogs & derivatives* / Q000378:metabolism*; D000737:Androstenols; D000818:Animals; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology; D004970:Estrone / Q000096:biosynthesis*; D007031:Hypothalamus / Q000196:embryology / Q000378:metabolism*; D007700:Kinetics; D051381:Rats
D000737:Androstenols; D003561:Cytarabine; D004970:Estrone; D000735:Androstenedione; C011482:19-hydroxy-4-androstene-3,17-dione