[Diagnosis of toxoplasmosis by indirect hemagglutination. I. Comparative study of fixation of glutaraldehyde on formolized erythrocytes of a soluble endogenous antigen, and insoluble particulate antigen and a total antigen].
Three different sorts of antigen are prepared ultrasonically: a soluble endogenous antigen, an insoluble particular antigen and a total mixed antigen. Formolinized red blood cells are sensibilized with glutaraldehyde. The authors study the sensitivity of the suspensions as a function of the different modes of antigen preparation. The results are compared with those obtained by immunofluorescence. The authors show the usefulness of total ultrasonic antigen which provides suspensions of great sensitivity easily and economically prepared.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000302:isolation & purification; D004912:Erythrocytes / Q000276:immunology; D005976:Glutaral; D006386:Hemagglutination Tests / Q000379:methods; D051379:Mice; D013010:Sonication; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology
D000941:Antigens; D005976:Glutaral
Diagnostic de la toxoplasmose par héhagglutination indirecte. I. Etude comparative de la fixation par le glutaraldéhyde sur des globules rouges formolés d'un antigène soluble, d'un antigène particulaire insoluble et d'un antigène total