[Operon for riboflavin synthesis in Bacillus subtilis. XI. Determination of the type of regulation using a test for dominance of operator-constitutive and regulator-constitutive mutations].
The strain GSY 468 of Bacillus subtilis, giving persistent unstable merozogotes was used as recipient in transformation. Diploid cells bearing a regulator constitutive or operator constitutive mutation were obtained, and their phenotype was studied. The operator constitutive mutation behaves as a dominant one, but the regulator constitutive is recessive. Hence the regulation type in riboflavin operon is negative. The product of the regulator gene is obviously a repressor protein.
D001412:Bacillus subtilis / Q000378:metabolism*; D005799:Genes, Dominant; D005808:Genes, Recessive; D009154:Mutation; D009876:Operon; D010641:Phenotype; D012256:Riboflavin / Q000096:biosynthesis*; D014170:Transformation, Genetic
D012256:Riboflavin
Issledovanie operona biosinteza riboflavina u Bacillus subtilis. Soobshchenie XI. Opredelenie tipa reguliatsii s pomoshch'iu testa na dominantnost' operator-konstitutivnykh i reguliator-konstitutivnykh mutatsiĭ