The effect of heat treatment on glucose metabolism in human kidney cell line.
The effect of supraoptimal temperature on various glucose metabolism pathways of human kidney cell line was studied. Exposure of cells to 44 degrees C for longer than one hour resulted in decreased activity of the tri-carboxylic-acid (Krebs) cycle, which hardly recovered at 72 hours. The first few steps of the pentose phosphate pathway were found to be much less sensitive to heat treatment than the whole cycle, and continued to be partially active even after a 3-hour exposure to 48 degrees C.
D002250:Carbon Radioisotopes; D002460:Cell Line; D002478:Cells, Cultured / Q000378:metabolism*; D002952:Citric Acid Cycle; D005947:Glucose / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney; D010428:Pentosephosphates; D013997:Time Factors
D002250:Carbon Radioisotopes; D010428:Pentosephosphates; D005947:Glucose