title: Periovulatory changes in ovarian prostaglandin formation and their hormonal control in the rat.
abstract: The concentration of prostaglandins of the E-group (PGE) and F-group (PGF) and the activity of prostaglandin-synthetase in rat ovaries increased on the evening of the day of proestrus and reached a peak at 5.00 h on the following morning, i.e. about the time of ovulation. Enzyme activity and PG concentrations receded to basal levels by 10.00 h on the day of estrus. These changes were prevented when the proestrous gonadotropin surge was blocked by administration of nembutal, and could be restored by administration of either LH or of FSH freed of LH contamination. The spontaneous preovulatory rise in prostaglandin concentration was about 6-fold for PGF and 30-fold for PGE, compared with values observed during the remainder of the cycle, whichas the rise in prostaglandin synthetase activity was only about 1.7-fold. The LH effect on PG accumulation had a latency of 2-4 h, which argues for enzyme synthesis rather than activation of preformed enzyme as the mechanism responsible. The small magnitude of the change in enzymic activity suggests that LH may, in addition, augment the availability of PG precursors. The results are compatible with the concept that prostaglandins play a physiological role in the gonadotropin-induced process of follicular rupture.
mesh_terms: D000818:Animals; D003864:Depression, Chemical; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010053:Ovary / Q000201:enzymology / Q000378:metabolism*; D010060:Ovulation / Q000187:drug effects; D010424:Pentobarbital / Q000494:pharmacology; D011247:Pregnancy; D011359:Proestrus; D011374:Progesterone / Q000097:blood; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism*; D011460:Prostaglandins F / Q000378:metabolism*; D051381:Rats; D013268:Stimulation, Chemical
chemical_list: D011458:Prostaglandins E; D011460:Prostaglandins F; D011374:Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D006899:Mixed Function Oxygenases; D011451:Prostaglandin-Endoperoxide Synthases; D010424:Pentobarbital; D007213:Indomethacin