title: Inhibition of RNA synthesis in Chlorella pyrenoidosa and Bacillus megaterium by the pine-blight toxin, dothistromin.
abstract: Dosthistromin, an anthraquinone derivative produced by the pine-blight fungus, Dothistroma pini, inhibits the growth of Chlorella pyrenoidosa and Bacillus megaterium. At growth inhibitory concentrations, dothistromin strongly inhibits incorporation of [3H]uridine into RNA of both species. With B. megaterium, marked inhibition of [3H]uridine incorporation is apparent within 5 min of addition of dothistromin, but only a slight inhibition of [3H]thymidine incorporation into the DNA-containing fraction or of [14C]leucine incorporation into protein is detectable after 10 min.
mesh_terms: D000880:Anthraquinones; D001410:Bacillus megaterium / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002708:Chlorella / Q000254:growth & development / Q000378:metabolism*; D005656:Fungal Proteins / Q000096:biosynthesis; D007930:Leucine / Q000378:metabolism; D003904:Mitosporic Fungi / Q000378:metabolism; D009183:Mycotoxins / Q000096:biosynthesis / Q000494:pharmacology*; D010649:Phenylalanine / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D012329:RNA, Bacterial / Q000096:biosynthesis*; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
chemical_list: D000880:Anthraquinones; D001426:Bacterial Proteins; D005656:Fungal Proteins; D009183:Mycotoxins; D012329:RNA, Bacterial; D010649:Phenylalanine; D012313:RNA; D007930:Leucine; D013936:Thymidine; D014529:Uridine