[Demonstration of an endo-beta-N-acetyl-glucosaminidase in rabbit serum].
An endo-beta-N-acetyl glucosaminidase of rabbit serum has been characterized after purification by affinity chromatography. The specificity was determined by fluorimetric monitoring of the hydrolysis of 4-methylumbelliferyl-beta-glycosides and by thin layer chromatography identification of the hydrolysis products of fluoresceinyl-beta-glycosides. This enzyme acts on the following oligosaccharides GlcNac-beta-1-4 leads to GlcNAc-beta-O-R giving free di-N-acetyl chitobiose and R-OH as hydrolytic products.
D000118:Acetylglucosaminidase / Q000097:blood* / Q000378:metabolism; D000818:Animals; D006596:Hexosaminidases / Q000097:blood*; D011817:Rabbits / Q000097:blood*
D006596:Hexosaminidases; D000118:Acetylglucosaminidase
Mise en évidence d'une endo-beta-N-acétyl-glucosaminidase du sérum de lapin