Plasma membrane: rapid isolation and exposure of the cytoplasmic surface by use of positively charged beads.
Erythrocytes were ionically attached to polylysine-coated beads 30 micrometers in diameter. The binding of the cells was so tenacious that lysis or disruption of the attached cells left the beads covered by plasma membranes whose cytoplasmic surface was exposed and accessible for further analysis.
D002458:Cell Fractionation; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D004910:Erythrocyte Membrane / Q000032:analysis / Q000502:physiology / Q000648:ultrastructure; D004912:Erythrocytes / Q000648:ultrastructure; D005898:Glass; D006801:Humans; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D008722:Methods; D010743:Phospholipids / Q000032:analysis
D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids