Genetic analysis of bacteriophage phi29 of Bacillus subtilis: mapping of the cistrons coding for structural proteins.
Four phage phi29 suppressor-sensitive mutants of cistron O have been examined for production of 14C-labeled viral-specific proteins in restrictive infections of Bacillus subtilis and fail to produce the protein of the viral neck lower collar. Cistrons O and F have been placed on the genetic map, containing 12 cistrons, by three-factor crosses. The phenotypes of five cistron J mutants have been analyzed by sodium dodecyl sulfate gel electrophoresis and autoradiography, and in three instances fragments of the normal polypeptide were detected. Three factor crosses with these mutants and a virus with a clear plaque phenotype were used to initiate the mapping of cistron J and the determination of the orientation of transcription in this map region.
D001412:Bacillus subtilis; D001435:Bacteriophages / Q000378:metabolism*; D002874:Chromosome Mapping; D005796:Genes; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000096:biosynthesis*
D014764:Viral Proteins