The interaction of four putative glutamate antagonists with glutamate and their effects on the toad spinal cord.
1. Four putative glutamate antagonists (L-glutamate diethyl ester, L-glutamate dimethyl ester, L-proline and 1-hydroxy-3-amino-pyrrolidone-2) were tested on the isolated hemisected toad spinal cord. 2. 1-hydroxy-3-amino-pyrrolidone-2 (10(-3)-10(-2) M) selectively antagonized the depolarizations  evoked in both dorsal and ventral roots by applications of L-glutamate (5 X 10(-4) M). 3 1-hydroxy-3-amino-pyrrolidone-2 also antagonized the depolarizations evoked in both dorsal and ventral roots by stimulation of the adjacent dorsal root. 4. The dimethyl and diethyl esters of L-glutamate and L-proline had their own depolarizing actions on the dorsal and ventral roots, and neither potentiated nor antagonized the effects of L-glutamate. 5. The results with 1-hydroxy-3-amino-pyrrolidone-2 offer further evidence for the involvement of L-glutamate and L-aspartate in synaptic transmission in the amphibian spinal cord.
D000818:Animals; D002024:Bufo marinus; D004347:Drug Interactions; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects; D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates / Q000494:pharmacology; D066298:In Vitro Techniques; D009467:Neuromuscular Depolarizing Agents / Q000494:pharmacology; D011392:Proline / Q000494:pharmacology; D011760:Pyrrolidinones / Q000494:pharmacology; D013116:Spinal Cord / Q000187:drug effects* / Q000502:physiology
D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates; D009467:Neuromuscular Depolarizing Agents; D011760:Pyrrolidinones; D011392:Proline