Bovine adrenal medullary chromogranin A: studies on the structure and further evidence for identity with dopamine-beta-hydroxylase subunit.
1. Chromogranin A was purified by the use of polyacrylamide gel electrophoresis. The amino acid composition of chromogranin A appeared to be nearly identical to that reported by other investigators and, moreover, was confirmed to be similar to that of dopamine beta-hydroxylase. 2. Dansyl-end group analysis revealed the presence of leucine as the only amino-terminal residue and quantitative estimations showed the presence of two leucine residues per molecule of 77 000 molecular weight. 3. Tryptic and CNBr patterns were obtained. Data are in good agreement with the concept of two nearly identical polypeptide chains per chromogranin A molecule of mol. wt 77 000. Patterns were compared with those obtained in parallel dopamine beta-hydroxylase and support the idea that chromogranin A and the dopamine beta-hydroxylase subunit are identical. Digestion with leucine amino peptidase gave further additional evidence for this suggestion. 4. Chromogranin A appeared to be free of carbohydrates. No cross-reaction was detected between chromogranin A and rabbit antibody against bovine adrenal dopamine beta-hydroxylase.
D000313:Adrenal Medulla / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D002864:Chromogranins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D003488:Cyanogen Bromide / Q000494:pharmacology; D004299:Dopamine beta-Hydroxylase / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007931:Leucyl Aminopeptidase / Q000494:pharmacology; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D011817:Rabbits / Q000276:immunology; D014357:Trypsin / Q000494:pharmacology
D000596:Amino Acids; D002241:Carbohydrates; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D004299:Dopamine beta-Hydroxylase; D007931:Leucyl Aminopeptidase; D014357:Trypsin; D003488:Cyanogen Bromide