Biochemical changes of ischemia.
Normothermic ischemic arrest by aortic cross-clamping, a widely used clinical technique, is associated with metabolic changes in the myocardium that are incompletely understood. The effects of aortic cross-clamping on glycolytic pathways as well as associated morphological changes are discussed. Emphasis is placed on the conservation of high-energy phosphate moieties during the period of cross-clamping as well as during reperfusion. A marked reduction in total high-energy phosphates (62%) and glycogen (63%) and an increase in lactate production (243%) denote a shift to anaerobic metabolism during the period of arrest. Despite reperfusion, total high-energy nucleotides remained depressed. The data suggest that persistent abnormal myocardial carbohydrate metabolism and low levels of high-energy nucleotides prevent recovery of contractility following normothermic ischemic arrest and reperfusion.
D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D004285:Dogs; D004734:Energy Metabolism; D006324:Heart Arrest, Induced / Q000379:methods*; D006331:Heart Diseases / Q000601:surgery*; D006801:Humans; D007431:Intraoperative Complications / Q000175:diagnosis*; D009203:Myocardial Infarction / Q000175:diagnosis*; D009206:Myocardium / Q000201:enzymology*
D003402:Creatine Kinase