Evidence of repair of DNA damage induced by 4-hydroxyaminoquinoline 1-oxide in guinea pig pancreatic slices in vitro.
In vitro exposure of guinea pig pancreatic slices to 4-hydroxyaminoquinoline 1-oxide (HAQO) resulted in increased [methyl-3H]thymidine ([3H]TdR) incorporation into DNA, both in the presence and absence of hydroxyurea  (HU). Normal DNA replicative synthesis, but not DNA repair synthesis, was suppressed by HU. The increase in [3H]TdR incorporation into  DNA damage induced by HAQO. Exposure of pancreatic slices to 10(-6) to 10(-5) M concentrations of HAQO did not significantly increase thymidine incorporation; however, a 15-min exposure to 10(-4) M HAQO induced a significant increase in HU-insensitive [3H]TdR incorporation into DNA. Kinetics of [3H]TdR incorporation suggests that most of the DNA repair synthesis occurs during the 2 hr following HAQO-induced DNA damage.
D015108:4-Hydroxyaminoquinoline-1-oxide / Q000494:pharmacology*; D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000634:Aminoquinolines / Q000494:pharmacology*; D000818:Animals; D002273:Carcinogens; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006918:Hydroxyurea / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D012038:Regeneration; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000634:Aminoquinolines; D002273:Carcinogens; D015108:4-Hydroxyaminoquinoline-1-oxide; D015112:4-Nitroquinoline-1-oxide; D013936:Thymidine; D006918:Hydroxyurea