Histopathologic evaluation of the effects of four calcium hydroxide liners on monkey pulps.
Pulpal response of four calcium hydroxide liners, MPC 10, MPC 12, Dycal and Pulpdent were tested on primary and permanent teeth with zinc oxide and eugenol (ZOE) and silicate as controls. Responses of the pulps were evaluatedi in Rhesus monkeys, utilizing Class V cavity preparations at 3 days, 5 and 8 weeks. An equivalent number of anterior and posterior teeth were studied for all compounds. The Ca(OH)2 liners, zinc oxide and eugenol (ZOE) and silicate controls were placed in 80 primary and 80 permanent teeth. Following perfusions the teeth were prepared utilizing routine histological procedures. The 3 day response of the calcium hydroxides was moderate with some disruption in the odontoblasts, vacuolization and mild inflammation underlying the cavity except Pulpdent which was more severe. At 5 weeks a decrease in inflammatory response and the formation of reparative dentin was similar for all calcium hydroxides tested at this time period. At 8 weeks more reparative dentin was noted with slight to moderate pulpal responses. At all time periods ZOE produced the least pulpal response while silicate produced the most severe response at 5 and 8 weeks. This study reports the biological responses of four calcium hydroxide compounds used as cavity liners in non-exposures in a series of primary and permanent teeth of monkeys using ZOE and silicate as controls. Responses to the four Ca(OH)2 compounds were moderate for all the experimental compounds except Pulpdent which was more severe at the early time period tested. ZOE produced a milder and silicate a severe response at all periods. All of these compounds were placed by random selection in anterior and posterior teeth of both arches and five teeth were evaluated in both primary and permanent teeth at 3 days, 5 and 8 weeks.
D000818:Animals; D002126:Calcium Hydroxide / Q000494:pharmacology*; D003736:Dental Cavity Lining; D003782:Dental Pulp / Q000187:drug effects* / Q000473:pathology; D003785:Dental Pulp Capping; D004353:Drug Evaluation, Preclinical; D000882:Haplorhini; D008253:Macaca mulatta; D012823:Silicate Cement / Q000494:pharmacology; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
D015035:Zinc Oxide-Eugenol Cement; D012823:Silicate Cement; D002126:Calcium Hydroxide