Low-frequency, pbsi-mediated plasmid transduction in Bacillus pumilus.
Three bacteriophages were tested for ability to transduce the plasmid of pPL10 between W mutant derivatives of Bacillus pumilus NRS 576. Phage PBP1- and PMB1-generated plasmid transductants occurred at about 10% the frequency of transductants for a chromosome marker. Phage PBS1-generated plasmid transductants occurred at less than 0.1% the frequency of transductants for a chromosome marker. Possible reasons for the extremely reduced capacity of PBS1 to generate plasmid transductants are discussed.
D001407:Bacillus / Q000032:analysis; D001412:Bacillus subtilis; D001435:Bacteriophages / Q000254:growth & development; D004269:DNA, Bacterial / Q000032:analysis; D004270:DNA, Circular / Q000032:analysis; D005111:Extrachromosomal Inheritance; D009154:Mutation; D010957:Plasmids; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D004270:DNA, Circular