Co-oxydation of a carotenoid by the enzyme lipoxygenase: influence on the formation of linoleic acid hydroperoxides.
A partially purified soybean lipoxygenase (L-3) was incubated for 15 min at pH 6.5 with linoleic acid and oxygen. Systems with and without the polyene glycoside crocin were compared. The system with crocin reacted with higher velocity with oxygen than did the control experiment without polyene. From the crocin 40% was destroyed. In the presence of crocin about 40% more linoleic acid hydroperoxides was formed than without the polyene but the linoleic acid break-down was equal in both experiments. L-3 peroxidises linoleic acid to 13L:13D:9L:9D-hydroperoxides in the proportions 43:11:21:25. In the presence of crocin the ratio of the isomers changed to 64:11:11:14.
D002338:Carotenoids / Q000378:metabolism*; D005519:Food Preservation; D008041:Linoleic Acids / Q000378:metabolism*; D008084:Lipoxygenase / Q000378:metabolism*; D009812:Odorants; D010084:Oxidation-Reduction; D013025:Glycine max; D013649:Taste
D008041:Linoleic Acids; D002338:Carotenoids; D008084:Lipoxygenase