Translocation in Bacillus subtilis: irreversible inactivation of elongation factor G and viability of a temperature- sensitive mutant.
A temperature-sensitive mutant of Bacillus subtilis 168 lost its viability irreversibly when grown at temperatures higher than 50 C. It is suggested that this loss of viability is due to irreversible alteration of elongation factor G activity, which was shown in vitro by two different assay methods.
D001412:Bacillus subtilis / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D002474:Cell-Free System; D002701:Chloramphenicol / Q000494:pharmacology; D002869:Chromosome Aberrations; D020963:GTP Phosphohydrolase-Linked Elongation Factors / Q000378:metabolism; D009154:Mutation; D010452:Peptide Biosynthesis; D010445:Peptide Elongation Factors; D011691:Puromycin; D013696:Temperature; D014178:Translocation, Genetic
D010445:Peptide Elongation Factors; D011691:Puromycin; D002701:Chloramphenicol; D020963:GTP Phosphohydrolase-Linked Elongation Factors