Isolation of aminopeptidase from Aspergillus flavus.
A mixture of aminopeptidase and neutral protease from the Aspergillus flavus mold obtained by chromatography on DEAE-Sephadex was fractionated by chromatography on the hydroxyalkyl methacrylate gel with chemically bonded 1,6 hexamethylene diamine and D-leucine. Aminopeptidase thus obtained was electrophoretically homogeneous. Conditions for chromatography were worked out allowing a one stage isolation of a highly active aminopeptidase sample directly from the alcoholic precipitate of the culture medium of the Aspergillus flavus mold.
D000626:Aminopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D001231:Aspergillus flavus / Q000201:enzymology*; D007700:Kinetics; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism
D010447:Peptide Hydrolases; D000626:Aminopeptidases