Properties of some norvaline-resistant mutants of Bacillus subtilis.
DL-Norvaline inhibits growth of wild-type Bacillus subtilis. A number of mutants resistant to growth inhibition by this analogue were isolated and studied. Cross-feeding experiments and paper chromatography of culture supernatants indicated that the mutants excreted leucine and possibly valine and glutamate. Enzymic analysis indicated that the mutants were derepressed for acetohydroxy-acid synthetase and alpha-isopropylmalate synthetase; however, no derepression of threonine deaminase, dihydroxyacid dehydrase or transaminase B was observed.
D015079:2-Isopropylmalate Synthase / Q000378:metabolism; D000095:Acetolactate Synthase / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology / Q000378:metabolism*; D002874:Chromosome Mapping; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004794:Enzyme Repression; D005796:Genes; D008040:Genetic Linkage; D005971:Glutamates / Q000096:biosynthesis; D006836:Hydro-Lyases / Q000378:metabolism; D007536:Isomerism; D007930:Leucine / Q000096:biosynthesis; D009154:Mutation; D009876:Operon; D013913:Threonine Dehydratase / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism; D014170:Transformation, Genetic; D014633:Valine / Q000096:biosynthesis / Q000494:pharmacology*
D003470:Culture Media; D005971:Glutamates; D000095:Acetolactate Synthase; D015079:2-Isopropylmalate Synthase; D000637:Transaminases; D006836:Hydro-Lyases; D013913:Threonine Dehydratase; D007930:Leucine; D014633:Valine