Synthesis and excretion of glycerol teichoic acid during growth of two streptococcal species.
Examination of both supernatant culture medium and cell pellets after exponential- and stationary-phase growth of Streptococcus mutans strain FA-1 and Streptococcus faecalis ATCC 9790 (S. faecium) showed the presence of [-3H]glycerol-labeled material that possessed several of the properties of glycerol teichoic acid. In the supernatant medium of S. mutans FA-1, an apparently large-molecular-size material, which eluted from agarose columns with the Kd value expected of a lipoteichoic acid, was observed. Large amounts of this material were present in supernatants during the stationary phase. In contrast, with S. faecalis only an apparently lower-molecular-weight form, with a Kd consistent with deacylated glycerol teichoic acid, was found in the growth medium. Both organisms had high-molecular-weight lipoteichoic acid in the cells along with the deacylated glycerol teichoic acid. The presence of relatively large amounts of glycerol teichoic acids in the medium was considered to be a result of excretion of these compounds rather than a result of cellular lysis.
D000143:Acids; D000906:Antibodies; D000907:Antibodies, Bacterial; D002474:Cell-Free System; D002854:Chromatography, Paper; D013293:Enterococcus faecalis / Q000254:growth & development / Q000378:metabolism*; D005990:Glycerol / Q000096:biosynthesis*; D006868:Hydrolysis; D007106:Immune Sera; D008970:Molecular Weight; D013291:Streptococcus / Q000254:growth & development / Q000378:metabolism*; D013682:Teichoic Acids / Q000096:biosynthesis; D014316:Tritium
D000143:Acids; D000906:Antibodies; D000907:Antibodies, Bacterial; D007106:Immune Sera; D013682:Teichoic Acids; D014316:Tritium; D005990:Glycerol