title: Interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61, substrate and beta-lactam antibiotics. A choice of models.
abstract: The interaction between the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61 and beta-lactam antibiotics is a multistep process during which a rather stable enzyme - antibiotic complex is formed. This mechanism of interaction is compatible with Lineweaver-Burk plots that are typical of a competitive inhibition of the hydrolysis of the peptide donor by the antibiotic. In fact, however, the same Lineweaver-Burk plots can be obtained on the basis of a non-competitive type of inhibition. At present, a choice between the two models cannot be made.
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D002228:Carbenicillin / Q000378:metabolism; D002268:Carboxypeptidases / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D008956:Models, Chemical; D010400:Penicillin G / Q000378:metabolism; D010404:Penicillin V / Q000378:metabolism; D010406:Penicillins / Q000378:metabolism*; D010458:Peptidyl Transferases / Q000378:metabolism*; D011485:Protein Binding; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D010406:Penicillins; D000217:Acyltransferases; D010458:Peptidyl Transferases; D002268:Carboxypeptidases; D002228:Carbenicillin; D010400:Penicillin G; D010404:Penicillin V