title: Mechanisms of streptomycin(SM)-resistance of highly SM-resistant Pseudomonas aeruginosa strains.
abstract: Three clinical isolates, K-Ps 94, K-Ps 97 and K-Ps 102, of Pseudomonas aeruginosa having R factor and showing MIC of more than 51,200 mcg/ml to streptomycin (SM), were examined for mechanisms of SM-resistance. Among the strains, K-Ps 94 and K-Ps 102 had R factor conferring SM-resistance. In K-Ps 94, the mechanism of SM-resistance was mainly owing to SM-phosphorylating enzyme and also owing to decreased permeability by an R factor, kR94. In K-Ps 97, it was considered to be due to SM-adenylylating enzyme by the chromosomal gene but not R factor, kR97. In K-Ps 102, the reduced permeability of the cell membrane to SM by an R factor, kR102, and the reduced affinity of the ribosome to the drug by the chromosomal gene contributed to the mechanisms of SM-resistance.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001681:Biological Assay; D002474:Cell-Free System; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004492:Edetic Acid / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism; D011815:R Factors; D013104:Spheroplasts / Q000187:drug effects; D013307:Streptomycin / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D003470:Culture Media; D000255:Adenosine Triphosphate; D004492:Edetic Acid; D013307:Streptomycin