An experimental model of Pseudomonas aeruginosa renal infection in rats.
The model of experimental pyocyanic renal infection in rats based on direct inoculation of bacteria to the renal parenchyma is described. The authors describe in detail the procedure and evaluation of the results with respect to microbiology, histology and reproducibility. The method produced a focal supurrative inflammation with a significant bacteriologica finding in renal tissue for 8 weeks at the least. The inflammation has a tendency to form focal cicatrices not accompanied by diffuse shrinkage of the kidney. The method is well reproducible and its technique is relatively simple thus permitting to induce infection in a large series of animals within a short period of time. In the second part of the paper, the use of this method in testing the therapeutical efficacy of gentamicin, carbenicillin and their combination is reported. The results showed gentamicin to be more effective than carbenicillin and the combination of the two to have the highest therapeutical effect. Moreover, their combination made it possible to reduce the dosage of the antibiotics and thereby reduce their potential undesirable effect. Further, the results showed the described model of experimental renal infection to be well suited for testing the efficacy of chemotherapeutics and resolving some problems of the treatment of infectious diseases.
D001437:Bacteriuria; D001835:Body Weight; D002228:Carbenicillin / Q000627:therapeutic use; D004195:Disease Models, Animal; D004341:Drug Evaluation; D004359:Drug Therapy, Combination; D005839:Gentamicins / Q000627:therapeutic use; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D011552:Pseudomonas Infections / Q000150:complications* / Q000188:drug therapy; D011550:Pseudomonas aeruginosa; D011704:Pyelonephritis / Q000188:drug therapy / Q000209:etiology* / Q000473:pathology; D013492:Suppuration
D005839:Gentamicins; D002228:Carbenicillin