Nucleotide sequences of the T1 and pancreatic ribonuclease digestion products from some large fragments of the 23S RNA of Escherichia coli.
When the 23S RNA from E. Coli was pretreated for 1 h at 60 degrees in the presence of Mg++ and K+ and then subjected to T1 ribonuclease attack, resistant fragments were recovered from 3 regions of the molecule: region A (containing 470-500 nucleotides) located at the 5' end of 23S RNA, region B (containing 520-550 nucleotides) located at the 3' end and region C (containing 110-120 nucleotides) lying between region A and region B. The nucleotide sequences of the T1 and pancreatic ribonuclease digestion products from these 3 regions have been studied and in most cases determined. In the course of these studies, a certain number of abnormal nucleotides, which are not methylated, have been encountered. A low level of sequence heterogeneity was detected.
D001483:Base Sequence; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis*; D005796:Genes; D006868:Hydrolysis; D008274:Magnesium; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D010179:Pancreas / Q000201:enzymology; D011188:Potassium; D011485:Protein Binding; D012329:RNA, Bacterial / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis*; D006163:Ribonuclease T1 / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D012265:Ribonucleotides / Q000032:analysis; D012270:Ribosomes; D013329:Structure-Activity Relationship
D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012265:Ribonucleotides; D012260:Ribonucleases; D006163:Ribonuclease T1; D008274:Magnesium; D011188:Potassium