Osmium-labeled polynucleotides. The reaction of osmium tetroxide with deoxyribonucleic acid and synthetic polynucleotides in the presence of tertiary nitrogen donor ligands.
Osmium tetroxide in the presence of pyridine or 2,2'-bipyridine has been found to react completely with the pyrimidine moieties (thymine, uracil, and cytosine) in polynucleotides. Pyrimidine osmate ester moieties, L2OSO4-pyrimidine, were formed. The OSO4 has added across the 5,6 double bond and L=pyridine or 1/2-bipyridine. The pyridine derivatives were not stable and decomposed slowly after the OSO4-pyridine reagent was removed by gel chromatography. Labeled poly(uridylic acid) lost osmium completely during gel chromatography unless the eluent contained a high concentration of pyridine. The products formed between OSO4-bipyridine and polynucleotides were much more stable and the OS label was retained during and after gel chromatography. Both the OSO4-pyridine and OSO4-bipyridine reagents reacted more rapidly than the OSO4-CN-reagent.
D001665:Binding Sites; D004247:DNA; D007700:Kinetics; D008024:Ligands; D009992:Osmium; D011089:Polydeoxyribonucleotides; D011131:Polyribonucleotides; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
D008024:Ligands; D011089:Polydeoxyribonucleotides; D011131:Polyribonucleotides; D009992:Osmium; D004247:DNA