[Structural transitions of the Torpedo cholinergic receptor in its membrane state as demonstrated by a fluorescent local anesthetic: quinacrine].
Differential fluorescence emissions of receptor-rich membrane fragments prepared from Torpedo marmorata electric organ and labelled by a fluorescent local anesthetic quinacrine are obtained by energy transfer. Addition of a cholinergic effector causes two classes of structural transitions, fast and slow, which are related to the agonist or antagonist character of the ligand.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004557:Electric Organ / Q000378:metabolism*; D005399:Fishes; D007700:Kinetics; D008566:Membranes; D010276:Parasympatholytics / Q000494:pharmacology; D011796:Quinacrine / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
D010276:Parasympatholytics; D011950:Receptors, Cholinergic; D002217:Carbachol; D011796:Quinacrine; D000109:Acetylcholine
Transitions structurales du récepteur cholinergique de Torpille dans son état membranaire mises en évidence à l'aide d'un anesthésique local fluorescent: la quinacrine