Alpha 1-acid glycoprotein in gastric cancer juice.
Gastric juice was neutralized (nGJ) in vivo by 80 ml of a phosphate buffer containing radiolabelled vitamin B12 as dilution indicator. Unprocessed nGJ was analyzed in the double gel diffusion technique for the presence of serum proteins using monospecific antisera. Alpha1-Acid glycoprotein (AGP) was found in a high incidence (36 out of 38 subjects) in nGJ of gastric cancer patients. AGP was also observed less frequently in nGJ of patients with Billroth II resections (6/15), metaplasia (11/52), gastric ulcer (3/24), chronic atrophic gastritis (2/26) and chronic gastritis (3/63). AGP was absent in the control group (0/21), in patients with surface gastritis (0/38) and in subjects with normal acid secretion (0/45). Immunochemical studies demonstrated no identity of AGP with human "gastrointestinal tumor associated antigens." In 7 out of 17 AGP positive samples immunochemical differences between gastric and serum AGP were observed.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm; D001646:Bile / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D004386:Duodenum; D005260:Female; D005743:Gastrectomy; D005750:Gastric Juice / Q000032:analysis*; D005756:Gastritis / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D005779:Immunodiffusion; D007419:Intestinal Secretions / Q000032:analysis; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D012463:Saliva / Q000032:analysis; D013274:Stomach Neoplasms / Q000032:analysis*; D013276:Stomach Ulcer / Q000378:metabolism
D000951:Antigens, Neoplasm; D001798:Blood Proteins; D006023:Glycoproteins