Distribution of RNA polymerase on Drosophila polytene chromosomes as studied by indirect immunofluorescence.
Using indirect immunofluorescence visualization techniques we investigated the in situ distribution of RNA polymerase B on Drosophila melanogaster polytene chromosomes. The enzyme was found at many sites distributed throughout the genome in a pattern clearly distinct from that observed for histone H1, but it was especially concentrated in puffs induced by heat shock.
D000818:Animals; D002875:Chromosomes / Q000201:enzymology*; D012321:DNA-Directed RNA Polymerases / Q000032:analysis*; D004331:Drosophila melanogaster; D005455:Fluorescent Antibody Technique; D006657:Histones / Q000032:analysis; D006358:Hot Temperature; D012319:RNA Polymerase II / Q000032:analysis*
D006657:Histones; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases