[A comparative study of total protein and albumin in man, monkey, dog and rat employing different analytical methods (author's transl)].
In the present study, serum albumin was determined colorimetrically in three animal species and in man with the help of four currently used dye-reagents (protein binding reagents). The results were compared with those obtained from corresponding electrophoretic and biuret determinations, using Versatol as a standard-control throughout. Using 2-(4-hydroxyazobenzene)-benzoic acid and bromocresol purple as reagent for the albumin determinations, species-specific differences from the electrophoretic results were found. No such differences occurred with bromo-cresol green, except in rats. There was no significant difference in albumin concentrations between man, monkeys and rats. Total protein concentration was only similar in man and monkeys.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D004285:Dogs; D004586:Electrophoresis / Q000379:methods; D005069:Evaluation Studies as Topic; D000882:Haplorhini; D006801:Humans; D007202:Indicators and Reagents; D051381:Rats; D012709:Serum Albumin / Q000032:analysis*; D013045:Species Specificity
D001798:Blood Proteins; D007202:Indicators and Reagents; D012709:Serum Albumin
Vergleichende Untersuchungen zur Bestimmung von Gesamtprotein und Albumin im Serum von Mensch, Affe, Hund und Ratte