title: Human pituitary growth hormone: a biologically active hendekakaihekaton peptide fragment corresponding to amino-acid residues 15-125 in the hormone molecule.
abstract: A hendekakaihekaton peptide fragment has been prepared by cyanogen bromide cleavage of the NH2-terminal 134-residue fragment of human pituitary growth hormone. It was characterized by amino-acid and end-group analyses, exclusion chromatography, disc electrophoresis, circular dichroism, and ultracentrifugation. The fragment, corresponding to amino-acid residues 15-125 in the hormone molecule, possesses hepatic ornithine decarboxylase (EC 4.1.1.17; L-ornithine carboxy-lyase) stimulating, lactogenic, and somatotrophic activity. It has immunoreactivity in the microcomplement-fixation and radioimmunoassay experiments. The circular dichroism data indicate that the hendekakaihekaton peptide fragment is devoid of secondary and tertiary structure.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D002850:Chromatography, Gel; D002942:Circular Dichroism; D010856:Columbidae; D003168:Complement Fixation Tests; D003426:Crop, Avian / Q000187:drug effects; D003488:Cyanogen Bromide; D003545:Cysteine / Q000032:analysis; D013006:Growth Hormone / Q000032:analysis* / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D010446:Peptide Fragments / Q000032:analysis; D011233:Precipitin Tests; D011487:Protein Conformation
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D013006:Growth Hormone; D003545:Cysteine; D003488:Cyanogen Bromide