Antiprotozoal activity in citrinin.
Citrinin has an inhibitory effect on growth of T. pyriformis. Citrinin also caused a shift to smaller cell size, particularly at the higher concentrations (25, 50 and 100 mug/ml). The mycotoxin exerted only a marginal effect on the respiration of T. pyriformis; but citrinin (25 mug/ml) induced an inhibitory effect on DNA, RNA and protein content. The greatest decrease was in RNA, while smaller decreases in protein and DNA were observed. A bioassay employing T. pyriformis was determined; the lower limit of citrinin detection was between 1 and 5 mug/ml.
D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D001578:Benzopyrans / Q000494:pharmacology*; D002953:Citrinin / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D009696:Nucleic Acids / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D014176:Protein Biosynthesis; D013769:Tetrahymena pyriformis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors
D000981:Antiprotozoal Agents; D001578:Benzopyrans; D009696:Nucleic Acids; D002953:Citrinin; D004121:Dimethyl Sulfoxide