title: Characterization of human dialyzable transfer factor from normal and chronic lymphoid leukemia sources.
abstract: Dialysates of lymphomonocytes can be roughly quantified through the use of optical density and measurements of RNA and protein contents. Dialysates of normal lymphocytes (N-TFd) contain about 224 +/- 67 mug of RNA-like material per 10(8) cells, 75 to 90% of which is eluted in fractions II and III on Biogel P10 chromatography. In contrast, dialysates of lymphocytes from patients suffering chronic lymphoid leukemia (CLL-TFd) contain approximately 12 times less RNA-like material (about 27 +/- 11 mug per 10(8) lymphocytes) and have a different characteristic chromatographic pattern. Bioassays with crude dialysates confirm (a) that N-TFd increases 3H-thymidine incorporation by nonsenstitized lymphocytes "in vitro" in the presence of PPD, and (b) that inbred Lewis rats develop positive skin tests (systemic transfer) after receiving injections of N-TFd or of fractions II and III from Biogel fractionation of N-TFd. Neither test gives positive results when CLL-TFd is used.
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D003956:Dialysis; D000882:Haplorhini; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood*; D008214:Lymphocytes / Q000378:metabolism; D010215:Papio / Q000097:blood; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew / Q000276:immunology; D012882:Skin Tests; D013056:Spectrophotometry, Ultraviolet; D013347:Subcellular Fractions / Q000032:analysis / Q000502:physiology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014165:Transfer Factor / Q000032:analysis* / Q000302:isolation & purification / Q000502:physiology; D014373:Tuberculin
chemical_list: D011506:Proteins; D014165:Transfer Factor; D014373:Tuberculin; D012313:RNA; D013936:Thymidine