[Enzymatic preparation of peptides of the general type: L-Ala-D-Glu-L-mesodiaminopimelyl (L)-D (140) Ala by transfer reactions between non radioactive corresponding peptides and D-alanine (140)].
Membrane bound LD-transpeptidase of Streptococcus faecalis ATCC 9790 was used to catalyse transpeptidation reactions between non radioactive peptide donors of the general type: L-Ala-D-Glu (L)meso-A2pm(L)-D-Ala and D-(14C) alanine acceptor. The presence of one or two amide residues on the carboxyl groups of glutamic acid and meso-diaminopimelic acid increases the transfer reactions and subsequently the yield in radioactive peptides L-Ala-D-Glu (L)meso-A2pm(L)-D-(14C) Ala.
D000217:Acyltransferases / Q000378:metabolism; D000409:Alanine; D002250:Carbon Radioisotopes; D003960:Diaminopimelic Acid; D013293:Enterococcus faecalis / Q000201:enzymology*; D005971:Glutamates; D007553:Isotope Labeling / Q000379:methods; D008722:Methods; D010452:Peptide Biosynthesis; D010458:Peptidyl Transferases / Q000378:metabolism
D002250:Carbon Radioisotopes; D005971:Glutamates; D003960:Diaminopimelic Acid; D000217:Acyltransferases; D010458:Peptidyl Transferases; D000409:Alanine
Préparation enzymatique de peptides du type L-Ala-D-Glu (L)meso-diaminopimélyl(L)-D-Ala(14C) par réactions d'échange entre les peptides correspondants non radioactifs et la D-alanine (14C)