Desaggregation of human red blood cells by various surface-active agents as related to changes of cell shape and hemolysis.
The effect of several surface-active substances (Na-oleate, Na-desoxycholate, Triton X 100, Pluronic F 38, Pluronic F 68, Pluronic F 108) on dextran-induced red cell aggregation (RCA) and red cell shape was investigated. Sedimentation measurements and photometric determinations of RCA indicated a desaggregating effect of oleate, Triton, F 38 and F 68. Shape changes were found in the presence of oleate and desoxycholate (crenation) and Triton (cup formation); in addition, these substances induced pronounced hemolysis. No hemolysis and no shape changes were found with any of the Pluronic polyols.
D001799:Blood Sedimentation; D002449:Cell Aggregation / Q000187:drug effects*; D003840:Deoxycholic Acid / Q000494:pharmacology; D003864:Depression, Chemical; D003911:Dextrans / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D009829:Oleic Acids / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011095:Polyethylenes / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D013268:Stimulation, Chemical; D013501:Surface-Active Agents / Q000494:pharmacology*
D003911:Dextrans; D006454:Hemoglobins; D009829:Oleic Acids; D011095:Polyethylenes; D013501:Surface-Active Agents; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D012964:Sodium