Activity of inhibitors on plasmatic erythropoietin and on the renal and splenic erythropoietic factors in the rabbit.
The author has studied the action of Cleland's reagent and Koshland's reagent on the erythropoietic factors present in the kidneys and the spleen of normal rabbits, in the plasma of bled rabbits and in the mixtures obtained through incubation of normal plasma with kidney and spleen homogenates. The results show that Cleland's reagent inhibits the activity of the renal and splenic erythro-stimulant factors, whereas it has no effect upon the erythropoietin in anaemic plasma and in plasma-kidney and plasma-spleen incubation mixtures. The activity of erythropoietin, on the other hand, is inhibited by Koshland's reagent, which has no influence on the renal and splenic erythro-stimulant factors.
D015077:2-Hydroxy-5-nitrobenzyl Bromide / Q000494:pharmacology*; D000818:Animals; D004229:Dithiothreitol / Q000494:pharmacology*; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism*; D009596:Nitrophenols / Q000494:pharmacology*; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects; D013154:Spleen / Q000378:metabolism
D009596:Nitrophenols; D004921:Erythropoietin; D015077:2-Hydroxy-5-nitrobenzyl Bromide; D004229:Dithiothreitol