Zeta-protentials of intact cell monolayers determined by electro-osmosis.
By measuring the electro-osmotic flow velocity of buffer through a glass capillary, on the inner surface of which cells have been grown, the zota-protential of these cells can be determined without the changes in zota-potential, viability and morphology caused by the chemical, enzymatic or mechanical pretreatment hitherto necessary for cell dispersion in the microelectrophoretic method. Cells grown in a monolayer inside the capillaries were: BGM, HEP-2, and RPMI 1846 (the last one also could be grown in suspension).
D002460:Cell Line; D002470:Cell Survival; D004492:Edetic Acid; D004586:Electrophoresis; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods*; D008564:Membrane Potentials; D014357:Trypsin
D004492:Edetic Acid; D014357:Trypsin