Chemical characterization of the regularly arranged surface layers of Clostridium thermosaccharolyticum and Clostridium thermohydrosulfuricum.
Clostridum thermosaccharolyticum and Clostridium thermohydrosulfuricum possess as outermost cell wall layer a tetragonal or hexagonal ordered array of macromolecules. The subunits of the surface layer can be detached from isolated cell walls with urea (8M) or guanidine-HCl (4 to 5 M). Triton X-100, dithiothreitol, ethylenediaminetetracetate, and KCl (3 M) had no visible effect on the regular arrays. Sodium dodecyl sulfate-polyacrylamide electrophroesis showed that, in both organisms, the surface layer is composed of glycoprotein of molecular weight 140,000. The glycoprotein from both microorganisms has a predominantly acidic amino acid composition and an acidic isoelectric point after isoelectric focusing on polyacrylamide gels. The glycocomponent is composed of glucose, galactose, mannose, and rhamnose.
D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D002473:Cell Wall / Q000032:analysis / Q000187:drug effects / Q000648:ultrastructure; D003013:Clostridium / Q000187:drug effects / Q000648:ultrastructure*; D004229:Dithiothreitol / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006146:Guanidines / Q000494:pharmacology; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D012210:Rhamnose / Q000032:analysis; D014508:Urea / Q000494:pharmacology
D000596:Amino Acids; D001426:Bacterial Proteins; D006023:Glycoproteins; D006146:Guanidines; D011092:Polyethylene Glycols; D011189:Potassium Chloride; D014508:Urea; D004492:Edetic Acid; D005947:Glucose; D008358:Mannose; D012210:Rhamnose; D004229:Dithiothreitol; D005690:Galactose