Cytochemical studies on T and B lymphocytes and lymphoblasts with special reference to acid phosphatase.
Lymphoblasts from ten patients with ALL or stage IV malignant lymphoma were studied cytochemically and investigated for rosette-forming capacity with sheep red blood cells (SRBC) as a T cell surface marker. SRBC binding capacity and acid phosphatase or beta-glucuronidase were tested simultaneously in single lymphocytes isolated from normal blood donors. Our results suggest that the presence of acid phosphatase (and beta-glucuronidase) represents a functional state of lymphocytes or lymphoblasts qualitatively independent of T cell differentiation, but quantitatively more pronounced in T cells than in B lymphocytes or non-T lymphoblasts.
D000135:Acid Phosphatase / Q000097:blood* / Q000276:immunology; D001402:B-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005966:Glucuronidase / Q000097:blood; D006801:Humans; D007158:Immunologic Techniques; D007223:Infant; D007945:Leukemia, Lymphoid / Q000201:enzymology* / Q000276:immunology; D008223:Lymphoma / Q000201:enzymology*; D008297:Male; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology
D000135:Acid Phosphatase; D005966:Glucuronidase