[Delta 5-3 beta hydroxysteroid dehydrogenase activity in the human full term placenta after culture: the effect of chorionic gonatropin].
The study made by histochemistry shows that the delta5-3 beta-hydroxysteroid-dehydrogenase (delta5-3 beta-HSDH) activity which is positive in the fresh placental tissue disappears after 24 h of culture but is maintained much longer if culture medium is supplemented with human chorionic gonadotropin (15 UI of HCG). The biochemical study confirms the delta5-3 beta-HSDH activity decrease during the first 24 h of culture. The enzymatic activity is restored during the following six days and this phenomenon is stimulated by the addition of HCG in the culture medium.
D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D036861:Delivery, Obstetric; D004790:Enzyme Induction; D005260:Female; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000096:biosynthesis / Q000378:metabolism*; D009924:Organ Culture Techniques; D010920:Placenta / Q000187:drug effects / Q000201:enzymology*; D011247:Pregnancy; D013997:Time Factors
D006063:Chorionic Gonadotropin; D006913:Hydroxysteroid Dehydrogenases
Activité de la delta5-3 beta hydroxystéroïde déshydrogénase dans le placenta humain à terme après culture: influence de l'hormone gonadotrope chorionique