The induction of delayed hypersensitivity in guinea pigs to poly U and poly A:U.
Guinea pigs were sensitized to poly U and poly A:U so that subsequent stimulation of spleen cells from these immunized animals with poly U and poly A:U resulted in the production of migration inhibitory factor (MIF). MIF was also produced when spleen cells from animals immunized with poly A:U were cultured in the presence of mycobacterial RNA or whole viable H37 Ra cells. Negative dermal reactions were observed when guinea pigs immunized with poly A, poly A:U were skin tested wtih these same synthetic nucleotides.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002464:Cell Migration Inhibition; D002474:Cell-Free System; D002478:Cells, Cultured; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007114:Immunization; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011063:Poly A-U; D011072:Poly U; D012329:RNA, Bacterial; D012882:Skin Tests; D013154:Spleen / Q000166:cytology; D014373:Tuberculin
D012329:RNA, Bacterial; D014373:Tuberculin; D011063:Poly A-U; D011072:Poly U