Arrangement and regulation of the nitrogen fixation genes in Klebsiella pneumoniae studied by depression kinetics.
Events underlying depression of the nitrogen fixation (nif) genes in Klebsiella pneumoniae M5A1 were analyzed in vivo by comparing the effects of selective inhibitors of transcription and translation on subsequent nitrogenase activity (rate of acetylene reduction). When batch cultures were induced for depression, an 87-min lag separated ammonium ion/oxygen removal and the appearance of activity.
D000114:Acetylene / Q000378:metabolism; D000643:Ammonium Chloride / Q000494:pharmacology; D002474:Cell-Free System; D002701:Chloramphenicol / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004794:Enzyme Repression; D005696:Galactosidases / Q000096:biosynthesis / Q000378:metabolism; D005796:Genes; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D009586:Nitrogen Fixation; D009591:Nitrogenase / Q000096:biosynthesis / Q000378:metabolism; D010084:Oxidation-Reduction; D014176:Protein Biosynthesis / Q000187:drug effects; D012293:Rifampin / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D000643:Ammonium Chloride; D002701:Chloramphenicol; D004492:Edetic Acid; D009591:Nitrogenase; D005696:Galactosidases; D000114:Acetylene; D012293:Rifampin