Thiostrepton-resistant mutants of Bacillus subtilis: localization of resistance to the 50S subunit.
A number of thiostrepton-resistant mutants of Bacillus subtilis were obtained. The thi mutations map proximally to strA. Effects of thiostrepton on polyphenylalanine synthesis with ribosomes of S-100 fractions from parent and mutant strains indicated that resistance was localized to the ribosomes. Furthermore, effects of thiostrepton on binding of [3H]GTP to ribosomes and 50S subunits from thiostrepton-sensitive and -resistant strains localized the site of resistance to the 50S subunit. In addition, revertants from thiostrepton-resistance to thiostrepton-sensitivity were obtained. Ribosomes and 50S subunits from these thiostrepton-sensitive revertants were sensitive to thiostrepton similar to parental sensitive B. subtilis.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects*; D001665:Binding Sites; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D009154:Mutation; D010649:Phenylalanine / Q000378:metabolism*; D011108:Polymers; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism*; D013883:Thiostrepton / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D011108:Polymers; D010649:Phenylalanine; D013883:Thiostrepton