Fractionation of RNA from tetahymena by affinity chromatography on poly-U-Sepharose.
Exponential growing Tetrahymena pyriformis organisms were labelled with (3H) uridine or (3H) adenosine. The labelled RNA was extracted and isolated by affinity chromatography on poly-uridylic-acid sepharose and further analysed by means of sucrose gradient centrifugation and RNase digestion. Experimental evidence proved the existence of RNase resistant poly adenylic-acid fragments in the RNA of Tetrahymena cells. This poly adenylic-acid segment has a sedimentation rate of 4-5 S and would be localised in the 10-12S region of the RNA which is probably the m-RNA.
D000818:Animals; D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D011061:Poly A / Q000032:analysis; D011072:Poly U; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D013769:Tetrahymena pyriformis / Q000378:metabolism*
D011061:Poly A; D011072:Poly U; D012313:RNA