Partial purification and properties of guanosine triphosphate cyclohydrolase from Drosophila melanogaster.
The first enzyme (named GTP cyclohydrolase) in the pathway for the biosynthesis of pteridines has been partially purified from extracts of late pupae and young adults of Drosophila melanogaster. This enzyme catalyzes the hydrolytic removal from GTP of carbon 8 as formate and the synthesis of 2-amino-4-hydroxy-6-(D-erythro-1',2',3'-trihydroxypropyl)-7,8-dihydropteridine triphosphate (dihydroneopterin triphosphate). Some of the properties of the enzyme are as follows: it functions optimally at pH 7.8 and at 42 C; activity is unaffected by KCl and NaCl, but divalent cations (Mg2+, Mn2+, Zn2+, and Ca2+) are inhibitory; the Km for GTP is 22 muM; and the molecular weight is estimated at 345,000 from gel filtration experiments. Of a number of nucleotides tested, only GDP and dGTP were used to any extent as substrate in place of GTP, and these respective compounds were used only 1.8% and 1.5% as well as GTP.
D000619:Aminohydrolases / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000201:enzymology*; D006136:GTP Cyclohydrolase / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D015032:Zinc / Q000494:pharmacology
D008345:Manganese; D012965:Sodium Chloride; D011189:Potassium Chloride; D000619:Aminohydrolases; D006136:GTP Cyclohydrolase; D008274:Magnesium; D015032:Zinc; D002118:Calcium