Isolation, growth and nitrogen fixation of a gas vacuole-less mutant of the blue-green alga Anabaena aphanizomenoides.
Mutants with loss of gas vacuoles (GVL-) were isolated from a clonal population of the gas vacuole containing (GVL+) blue-green alga Anabaena aphanizomenoides with a spontaneous mutation frequency of 1.7 x 10(-3) in nitrogen-free media. Growth and nitrogen fixation of the mutant were slow when compared to the GVL+ parent. Both the parent and mutant filaments tolerated 0.02 microgram/ml of streptomycin, and higher concentrations were lethal. The reversion of the mutant towards the parent did not occur.
D000458:Cyanobacteria / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D005740:Gases; D009154:Mutation; D009586:Nitrogen Fixation; D014617:Vacuoles / Q000502:physiology
D005740:Gases