Reversal of morphological differentiation of mouse neuroblastoma cells by mitosis-inhibitors and anesthetics.
Differentiated mouse neuroblastoma cells (C 1300) were exposed to various mitosis-inhibitors (vinblastine, colchicine and griseofulvin) and substances with anesthetic action (lidocaine, tetracaine, chlorpromazine and sodium dodecyl sulphate). All the drugs caused rapid retraction of the neurites, which was reversible in all cases but for sodium dodecyl sulphate, and showed a sigmoid dose-response relationship. The two groups of substances caused morphologically similar effects in that the microtubules disappeared and the intracellular orientation was lost. The order of potency of the anesthetics corresponded to their efficiency to cause nerve-block and antihemolysis as reported by others. Colchicine, griseofulvin, lidocaine and chlorpromazine were tested for effects of agglutination of undiffentiated cells. They inhibited agglutination at doses that were only slightly higher than those causing neurite retraction. The possibility of a close relationship between the cell membrane and microtubule system will be considered.
D000818:Animals; D002449:Cell Aggregation; D002454:Cell Differentiation; D002746:Chlorpromazine / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D006118:Griseofulvin / Q000494:pharmacology; D008012:Lidocaine / Q000494:pharmacology; D051379:Mice; D008870:Microtubules; D008938:Mitosis / Q000187:drug effects; D009443:Neuritis; D009447:Neuroblastoma / Q000473:pathology*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013748:Tetracaine / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology
D013748:Tetracaine; D006118:Griseofulvin; D012967:Sodium Dodecyl Sulfate; D014747:Vinblastine; D008012:Lidocaine; D003078:Colchicine; D002746:Chlorpromazine