[A general method for the determination of the conformation of nucleotides bound to an enzyme site. Interaction between glycogen phsophorylase b and nucleoside-5'-monophosphates].
The conformation of 5' nucleotides in the active site of glycogen phosphorylase b has been deduced from T2 relaxation time of protons H1, H8 and H2. It is shown by deuterium substitution of the purine ring in position 8 that the orientation of the base is anti in the case of strong activators like adenosine 5'-monophosphate, and syn in that of weak activators like inosine 5'-monophosphate.
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives; D000495:Allosteric Site; D003903:Deuterium; D004789:Enzyme Activation; D006150:Guanine Nucleotides; D007292:Inosine Nucleotides; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008722:Methods; D008968:Molecular Conformation; D006005:Phosphorylases; D011685:Purine Nucleotides; D013329:Structure-Activity Relationship
D006150:Guanine Nucleotides; D007292:Inosine Nucleotides; D011685:Purine Nucleotides; D000249:Adenosine Monophosphate; D003903:Deuterium; D006005:Phosphorylases
Une méthode générale de détermination de la conformation des nucléotides liés à un site enzymatique. Interaction entre la glycogène phosphorylase b et les nucléoside-5'-monophosphates