Mapping of the gene specifying DNA polymerase III of Bacillus subtilis.
polC, the gene specifying the structure of the replication-specific DNA polymerase III of B. subtilis, was mapped by exploiting azp-12, a mutation conferring resistance to azopyrimidine which determines a mutant, azopyrimidine-resistant enzyme. azp-12 was located in the area of the pyrA locus and is between spcB1 and recA1. azp-12 was linked by transformation to four other mutations which influence the in vitro behaviour of DNA polymerase III--polC25, polC26, mut-1(ts), and DNAF133; the close linkage of these five mutations strongly suggests that they are alleles of the same gene.
D001391:Azo Compounds / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D005796:Genes; D008040:Genetic Linkage; D005838:Genotype; D010641:Phenotype; D011743:Pyrimidines / Q000494:pharmacology; D014161:Transduction, Genetic
D001391:Azo Compounds; D011743:Pyrimidines; D004254:DNA Nucleotidyltransferases