[Establishment of a primary culture of cells from embryonic tissue of Loliyo vulgaris L.(Cephalopod)].
The author studies the behaviour in vitro of organ fragments of Loligo vulgaris embryos at hatching stage. These fragments are maintained in three different media. The time of survival of cells is 20 to 25 days in the medium I(sea water), 40 days in the medium II(perivitellin fluid) and 70 to 80 days in the medium II(perivetellin fluid + horse serum). It is this medium III that the original explant is transformed into a typical primary cell culture.
D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D049832:Decapodiformes / Q000196:embryology; D004625:Embryo, Nonmammalian / Q000166:cytology; D004847:Epithelial Cells; D005347:Fibroblasts; D012623:Seawater
D003470:Culture Media
Mise au point d'une culture primaire de cellules à partir du tissu embryonnaire de Loligo vulgaris L. (Céphalopode)