Preliminary observations on the protective action of a cytoplasmic immunogen of Neisseria gonorrhoeae.
A non-toxic protein, termed beta(-t), consisting of lysine, aspartic acid, threonine, serine, glutamic acid, glycine, and alanine (1.2:1.3:1.3:3:2.2:7:1) was isolated, by the polyacrylamide gel electrophoresis, from an isoelectric toxic proteinaceous fraction beta(+1) of the type 1 cytoplasm of N. gonorrhoeae. The beta(-t) antigen elicited delayed hypersensitivity and vigorous primary and secondary humoral responses in rabbits, and it conferred immune protection against intraocular gonococcal infections.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000633:toxicity; D001426:Bacterial Proteins / Q000276:immunology* / Q000633:toxicity; D002458:Cell Fractionation; D002642:Chick Embryo; D003315:Cornea; D004591:Electrophoresis, Polyacrylamide Gel; D006069:Gonorrhea / Q000517:prevention & control*; D006968:Hypersensitivity, Delayed; D007114:Immunization; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007267:Injections; D007271:Injections, Intradermal; D007279:Injections, Subcutaneous; D007525:Isoelectric Focusing; D007928:Lethal Dose 50; D008297:Male; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D011817:Rabbits; D013053:Spectrophotometry
D000596:Amino Acids; D000942:Antigens, Bacterial; D001426:Bacterial Proteins