Protease and peptidase activities in growing and sporulating cells and dormant spores of Bacillus megaterium.
Peptidase and protease activities on many different substrates have been determined in several stages of growth of Bacillus megaterium. Extracts of log-phase cells, sporulating cells, and dormant spores of B. megaterium each hydrolyzed 16 different di- and tripeptides. The specific peptidase activity was highest in dormant spores, and the activity in sporulating cells and log-phase cells was about 1.2-fold and 2- to 3-fold lower, respectively. This peptidase acticity was wholly intracellular since extracellular peptidase activity was not detected throughout growth and sporulation. In contrast, intracellular protease activity on a variety of common protein substrates was highest in sporulating cells, and much extracellular activity was also present at this time. The specific activity of intracellular protease in sporulating cells was about 50- and 30-fold higher than that in log-phase cells and dormant spores, respectively. However, the two unique dormant spores proteins known to be the major species degraded during spore germination were degraded most rapidly by extracts of dormant spores, and slightly slower by extracts from log-phase or sporulating cells. The specific activities for degradation of peptides and proteins are compared to values for intracellular protein turnover during various stages of growth.
D000418:Albumins / Q000378:metabolism; D000813:Anilides / Q000378:metabolism; D001391:Azo Compounds / Q000378:metabolism; D001410:Bacillus megaterium / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D002250:Carbon Radioisotopes; D002364:Caseins / Q000378:metabolism; D002474:Cell-Free System; D004151:Dipeptides / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D006868:Hydrolysis; D009842:Oligopeptides / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D013171:Spores, Bacterial / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism
D000418:Albumins; D000813:Anilides; D001391:Azo Compounds; D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D002364:Caseins; D004151:Dipeptides; D006454:Hemoglobins; D009842:Oligopeptides; D010450:Endopeptidases; D010447:Peptide Hydrolases