Reaction of human smooth muscle antibody with human platelets.
Platelets, prepared from fresh human platelet-rich plasma smeared on slides and stained with human serum containing smooth muscle antibodies (SMA) in indirect IFL, showed a bright cytoplasmic fluorescence with numerous projections extending from the surface. A prerequisite for obtaining a positive reaction with SMA-positive serum was that a chelating agent was present in the suspending medium when preparing the smears. The projections could be demonstrated also by anti-HeLa cell (anti-species) serum. This indicates that the projections had a membraneous cover. Staining of live platelets was always negative. Platelets treated with cytochalasin B for 1 hr were smooth and spherical and did not show any surface projections.
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D001792:Blood Platelets / Q000187:drug effects / Q000276:immunology*; D003571:Cytochalasin B / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005976:Glutaral / Q000494:pharmacology; D006367:HeLa Cells / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D009130:Muscle, Smooth / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D007106:Immune Sera; D003571:Cytochalasin B; D005976:Glutaral