title: The humoral response to DNCB: its detection by different techniques.
abstract: Antibodies in the sera of guinea pigs immunized with DNCB were detected by Ouchterlony-type analyses and were estimated in weight units by the mABC procedure. Assays of these sera by means of passive cutaneous anaphylaxis, passive hemolysis, or the ABC-33 antigen-binding technique were essentially negative. The validity of the mABC analyses was established by comparison of results obtained in quantitative precipitation studies with sera of guinea pigs immunized with DNP-BGG. In these instances, also, the ABC-33 assays failed to detect all the anti-DNP immunoglobulins.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001666:Binding Sites, Antibody; D004137:Dinitrochlorobenzene / Q000276:immunology*; D006168:Guinea Pigs; D006461:Hemolysis; D007104:Immune Adherence Reaction; D007114:Immunization; D005779:Immunodiffusion; D007158:Immunologic Techniques; D009578:Nitrobenzenes / Q000276:immunology*; D010323:Passive Cutaneous Anaphylaxis; D011233:Precipitin Tests
chemical_list: D000906:Antibodies; D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes