The application of polyvalent horse immune sera for electroimmunodiffusion methods.
Horse immune sera do not give satisfactory results in immunochemical techniques based on electrophoresis of antigens through antibody-containing agarose gel. As the majority of precipitating horse antibodies belongs to the beta globulins, they migrate in the gel during electrophoresis. After enzymatic treatment the pepsin fragments work well in all electroimmunodiffusion methods.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D006736:Horses; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis / Q000379:methods
D000906:Antibodies; D007106:Immune Sera