Effects of prolactin and growth hormone on DNA synthesis of rat mammary carcinomas in vitro.
Explants derived from mammary carcinomas of DMBA-treated female Sprague-Dawley rats were cultured for 5 days in Medium 199 containing insulin and corticosterone. The addition of ovine prolactin to the culture media resulted in a consistent significant increase in H3-thymidine incorporation into DNA. DNA synthesis of explants treated with either ovine or human growth hormone was intermediary to prolactin-treated cultures and control cultures. A combination of prolactin and human growth hormone often increased DNA synthesis above either hormone alone, suggesting a possible growth synergism between these peptides.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology*; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D009924:Organ Culture Techniques; D011388:Prolactin / Q000494:pharmacology*; D051381:Rats
D004273:DNA, Neoplasm; D015127:9,10-Dimethyl-1,2-benzanthracene; D011388:Prolactin; D013006:Growth Hormone