Use of the blocking procedure in mixed hemadsorption tests for the analysis of surface antigens in liver tissue culture cells.
For detection of surface species-specific and specific tumor antigens in the various tissue culture cell lines, including interspecies hybrid cell line, the blocking procedure was included in a microhemadsorption test (MHT). Use of the appropriate high-titered blocking antisera unadequate to the indicatory system of MHT permits the detection of different species-specific and tumor antigens on the surface of the liver tissue culture cells with the use of heterologous unabsorbed immune sera.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis; D001667:Binding, Competitive; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D002522:Chlorocebus aethiops; D006224:Cricetinae; D000882:Haplorhini; D006382:Hemadsorption; D006383:Hemadsorption Inhibition Tests; D007106:Immune Sera; D051379:Mice; D013045:Species Specificity
D000941:Antigens; D000951:Antigens, Neoplasm; D007106:Immune Sera