Polychlorinated biphenyls (phenoclor and pyralene) and intestinal transport of hexoses and amino acids in mice.
1. Using mouse everted whole small intestine 7 days after a single dose of Phenoclor DP6 (hexachlorobiphenyl) at 1.0 g kg-1 body wt, absorption of D-glucose decreased significantly, probably owing to toxic traces of pentodicholobenzofuran in the PCB preparation and not its high chlorine content. D-glucose tissue accumulation decreased. The serosal fluid transfer also fell but not the gut fluid uptake. D-glucose absorption and fluid transfer remained unchanged following doses of other Phenoclor and Pyralene (PCB) compounds. 2. Using DP6, malabsorption of D-glucose and impaired serosal fluid transfer were not evident 7 days after a single dose of 0.1, 0.25 and 0.50 g kg-1 body wt. One to 21 days after a single dose (1.0 g kg-1 body wt) of DP6, D-glucose absorption and serosal fluid transfer decreased from 3 to 14 days but thereafter became normal. 3. Although absorption of D-galactose, D-glucose following membrane hydrolysis of D-maltose, and L-arginine, L-histidine, L-ornithine and L-proline decreased slightly 7 days after a single dose of Phenoclor DP6,the results were not significant, nor were changes in fluid transfer. 4. Following Phenoclor DP6-treatment, D-glucose malabsorption was abolished by an exogenous energy supply (D-mannose). DP6 affected intracellular metabolism and not the glucose carrier at the membrane brush border. 5. The body-weights and fresh small intestinal weights of mice treated with different PCBs remained unchanged after seven days. The histology of the small intestine showed minimal changes.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D005260:Female; D005947:Glucose / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism*; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects*; D007422:Intestines / Q000033:anatomy & histology; D008297:Male; D051379:Mice; D011078:Polychlorinated Biphenyls / Q000494:pharmacology* / Q000633:toxicity; D013997:Time Factors
D000596:Amino Acids; D006601:Hexoses; C013191:phenoclor; C013226:pyralene; D011078:Polychlorinated Biphenyls; D005947:Glucose