Transformation-dependent modifications in released and cell-bound surface proteins detected by antisera to shed antigens.
Antiserum against antigens released into the medium by rat cell cultures that express the transformation phenotype has been shown to produce a complement-mediated lysis, not only in the cells that liberate such antigens but to a greater extent in cells that are phenotypically restricted in the expression of transformation properties. Reaction of the antibody with preiodinated surface proteins released into the medium reveals that the expression of transformation leads to a greater degradation of the external proteins shed into the medium.
D000951:Antigens, Neoplasm; D002462:Cell Membrane / Q000032:analysis*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003165:Complement System Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*
D000951:Antigens, Neoplasm; D002860:Chromium Radioisotopes; D009363:Neoplasm Proteins; D003165:Complement System Proteins