[Preparation and characterization of glycopeptides from human monoclonal immunoglobulin].
The carbohydrate composition of an human IgM myeloma protein (IgM Du) has been determined. Seventeen homogeneous glycopeptides are described and exhibit a very large microheterogeneity. They appear as two different groups : the first one contains only mannose and N-acetyl glucosamine, while the other contains N-acetyl-glucosamine, mannose, galactose, fucose, and sialic acid in variable amounts. One glycopeptide termed IX1, which contains 6 mannose and 1 N-acetylglucosamine residues is located on the terminal portion of the Fc fragment and its aminoacid sequence has been determined : Tyr-Asx-Val-Ser.
D000117:Acetylglucosamine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D006020:Glycopeptides / Q000032:analysis*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D008358:Mannose / Q000032:analysis; D009194:Myeloma Proteins / Q000097:blood*; D012794:Sialic Acids / Q000032:analysis; D008258:Waldenstrom Macroglobulinemia / Q000097:blood*
D000596:Amino Acids; D006020:Glycopeptides; D007075:Immunoglobulin M; D009194:Myeloma Proteins; D012794:Sialic Acids; D005643:Fucose; D008358:Mannose; D000117:Acetylglucosamine; D005690:Galactose
Préparation et caractérisation des glycopeptides d'une immunoglobuline monoclonale humaine