In vivo and in vitro effects of dimethylbenz(a)anthracene on nuclear uptake of estrogen-receptor complex in rat uterus.
A single oral carcinogenic (20 mg) dose of 7,12-dimethylbenz(a)anthracene (DMBA) significantly reduced the uterine nuclear uptake of estrogen-receptor complex with immature Sprague-Dawley rats were injected with 0.05 microng of tritiated estradiol-17beta (E2) 24 hours after DMBA. Incubation experiments with whole uteri or with isolated uterine nuclei showed that DMBA in vitro does not act by reducing estrogen-receptor transformation or transport into nuclei. DMBA may act in vivo by stimulating hepatic degradation of injected estradiol-17beta so that less active form of the steroid reaches target tissues.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000818:Animals; D001551:Benz(a)Anthracenes / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D003864:Depression, Chemical; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D051381:Rats; D011960:Receptors, Estrogen / Q000378:metabolism*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D001551:Benz(a)Anthracenes; D011960:Receptors, Estrogen; D004958:Estradiol; D015127:9,10-Dimethyl-1,2-benzanthracene