title: Oestradiol stimulation of prolactin release from canine pituitary in culture.
abstract: Anterior canine pituitaries were maintained in tissue culture for 8 days, and the immunoreactive prolactin released, was measured by a heterologous radioimmunoassay for canine prolactin. Luteinizing hormone-releasing hormone (LH-RH) and thyrotrophic hormone-releasing hormone (TRH) did not affect prolactin release, while theophylline and oestradiol-17 beta stimulated the release of canine prolactin.
mesh_terms: D000818:Animals; D046508:Culture Techniques; D004285:Dogs; D004958:Estradiol / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D012636:Secretory Rate / Q000187:drug effects; D013806:Theophylline / Q000494:pharmacology; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology
chemical_list: D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D013973:Thyrotropin-Releasing Hormone; D011388:Prolactin; D013806:Theophylline