Transformation of leucine and rifampin traits in Neisseria gonorrhoeae with deoxyribonucleic acid from homologous and heterologous origins.
A leucine-requiring, rifampin-sensitive strain of Neisseria gonorrhoeae was transformed to a leucine-nonrequiring, rifampin-resistant phenotype with deoxyribonucleic acid (DNA) obtained from both N. meningitidis and N. gonorrhoeae. The transforming efficiency of the meningococcal DNA was about 10- to 100-fold less than that of the homologous gonococcal DNA. A chemically defined medium that would support growth of most gonococcal isolates was used as a complete medium. A minimal medium was used for selection of Leu+ transformants. N-methyl-N'-nitro-N-nitrosoguanidine was used as a mutagen for isolating leucine prototrophs from leucine-requiring isolates of N. gonorrohoeae.
D003470:Culture Media; D004269:DNA, Bacterial; D007930:Leucine / Q000378:metabolism*; D009153:Mutagens; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000378:metabolism; D009345:Neisseria meningitidis; D009604:Nitrosoguanidines; D012293:Rifampin / Q000494:pharmacology*; D013045:Species Specificity; D014170:Transformation, Genetic
D003470:Culture Media; D004269:DNA, Bacterial; D009153:Mutagens; D009604:Nitrosoguanidines; D007930:Leucine; D012293:Rifampin