title: [Problems in obtaining antiserum to staphylococcal enterotoxin D].
abstract: Experimentally were demonstrated the difficulties overcome and the possibilities of producing a Staphylococcus-enterotoxin D antiserum of a 1:80 titer. The product was shown to be suitable for work as well as for further studies. Given are the schemes of immunizing a rabbit serving as a serum producer and those for purifying the antiserum (eliminating heterologous antibodies), its titration with a microimmunodiffusion test, etc. In this connection some problems are referred to concerning the production of the amounts necessary of enterotoxin D, its purification and toxicity for test animals, etc., that are successfully solved by the authors.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000992:Antitoxins / Q000302:isolation & purification; D004768:Enterotoxins; D005779:Immunodiffusion; D011817:Rabbits; D013210:Staphylococcus / Q000276:immunology*
chemical_list: D000992:Antitoxins; D004768:Enterotoxins
vernacular_title: Problemi pri poluchavaneto na stafilokokov enterotoksin-D--antiserum