Effect of cytochalasin B on the adhesion of mouse peritoneal macrophages.
The adhesion of normal mouse macrophages to glass surfaces was reduced by nontoxic levels (1-50 mug/ml) of cytochalasin B in combination with a centrifugal force (1,000-8,000 g). Macrophages nonspecifically activated by Corynebacterium acnes were also detached by this treatment, but less effectively. The effects of cytochalasin B treatment on these cells were shown to be reversible. After detachment, the cells reattached to glass, appeared morphologically normal, and behaved like untreated cells as judged by adhesion, acid phosphatase levels, and phagocytosis. The effect of cytochalasin B on several parameters of phagocytosis by normal macrophages was also examined. The results demonstrate that cytochalasin B can be used to detach macrophages from surfaces and suggest a functional relationship between phagocytosis and macrophage adhesion to surfaces. Furthermore, the effect of cytochalasin B on adhesion of phagocytic cells provides a probe for further investigation of the adhesion of cells to surfaces.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D004731:Endotoxins / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D011425:Propionibacterium acnes; D014357:Trypsin / Q000494:pharmacology
D004731:Endotoxins; D003571:Cytochalasin B; D004492:Edetic Acid; D000135:Acid Phosphatase; D014357:Trypsin