Regulated in vitro synthesis of the enzymes of the deo operon of Escerichia coli. properties of the DNA directed system.
The four enzymes deoxyriboaldolase, thymidine phosporylase, deoxyribomutase, and purine nucleoside phosphorylase have been synthesized in substantial amounts in a DNA-dependent in vitro system programmed with DNA containing the deo operon. The synthesis is greatly stimulated by deoxyribose-5-phosphate and cyclic AMP indicating that the deoR repressor and the catabolite activating protein (CAP) are highly active under our cell-free conditions. In contrast it has not yet been possible to observe a reproducible effect of the cytR repressor in vitro. The sequential appearance of active enzymes has confirmed the direction of transcription as being dra-tpp-drm-pup and has indicated that the four genes are transcribed into a single tetracistronic message.
D000446:Aldehyde-Lyases / Q000032:analysis; D002474:Cell-Free System; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000032:analysis*; D003855:Deoxyribose; D004926:Escherichia coli / Q000201:enzymology*; D005809:Genes, Regulator; D005958:Glucosephosphates; D009876:Operon; D010428:Pentosephosphates; D010770:Phosphotransferases / Q000032:analysis; D011683:Purine-Nucleoside Phosphorylase / Q000032:analysis; D012267:Ribosemonophosphates; D013939:Thymidine Phosphorylase / Q000032:analysis
D004269:DNA, Bacterial; D005958:Glucosephosphates; D010428:Pentosephosphates; D012267:Ribosemonophosphates; D003855:Deoxyribose; D011683:Purine-Nucleoside Phosphorylase; D013939:Thymidine Phosphorylase; D010770:Phosphotransferases; D000446:Aldehyde-Lyases; C020019:deoxyribose-phosphate aldolase