Demonstration of cell division by septation in a variety of gram-negative rods.
Through use of an initial fixative employing a combination of crotonaldehyde and glutaraldehyde, septa were preserved in thin sections of dividing cells of strains of Pseudomonas aeruginosa, Salmonella typhimurium, Shigella sonnei, and Escherichia coli when grown at 30 C in a dilute basal medium. The same procedures, however, revealed only a constrictive division process in Proteus vulgaris and Erwinia sp. This adds to the evidence that septation, although difficult to demonstrate, is the process of cell division in the enteric gram-negative rods and the pseudomonads and that constriction is a fixation artifact in these organisms.
D000447:Aldehydes; D001419:Bacteria / Q000254:growth & development* / Q000648:ultrastructure; D002455:Cell Division; D002462:Cell Membrane / Q000648:ultrastructure; D002473:Cell Wall / Q000648:ultrastructure; D004885:Erwinia / Q000648:ultrastructure; D004926:Escherichia coli / Q000648:ultrastructure; D008854:Microscopy, Electron; D010457:Peptidoglycan; D011514:Proteus vulgaris / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000648:ultrastructure; D012486:Salmonella typhimurium / Q000648:ultrastructure; D012764:Shigella sonnei / Q000648:ultrastructure
D000447:Aldehydes; D010457:Peptidoglycan