A modified method for tissue localization of cells bearing a complement receptor.
A modification of the method originally described by Dukor et al. (1970) was used for localization of cells bearing a complement receptor in lymphoid tissue of man, guinea pig, mouse and rat. Optimal fixation of the adherent indicator erythrocytes to the tissue section and satisfactory morphology of the tissue were obtained with a mixture of 1% paraformaldehyde and 0.5% glutaraldehyde. In addition, selective staining of indicator erythrocyte hemoglobin with Amido Black resulted in high contrast with the tissue constituents. The combination of fixative and stain descirbed above permitted more precise localization of cells bearing complement receptors in tissue sections.
D000580:Amido Black; D000818:Animals; D001666:Binding Sites, Antibody; D003165:Complement System Proteins / Q000502:physiology*; D005557:Formaldehyde; D005976:Glutaral; D006168:Guinea Pigs; D006801:Humans; D007104:Immune Adherence Reaction / Q000379:methods*; D008198:Lymph Nodes / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology*; D051379:Mice; D014066:Palatine Tonsil / Q000276:immunology; D051381:Rats; D011955:Receptors, Drug; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D011955:Receptors, Drug; D005557:Formaldehyde; D003165:Complement System Proteins; D000580:Amido Black; D005976:Glutaral