Radioimmunoassay for human T-cell associated antigens on MOLT 4 cultured cells.
We have detected antigens associated with two malignant T-cell lines by use of a sensitive radioimmunoassay. Baboon antisera to a cultured malignant T cell line (MOLT 4F) were prepared and the gammaG-anti-MOLT 4F was enriched by specific adsorption onto, and elution from, MOLT 4F cells followed by repeated absorption with pools of 21 established, long-term cultured B cell lines. Purified 125I-gammaG-anti-MOLT 4F preparation was tested for relative binding to autologous (MOLT 4F) and allogenetic (Sommer-RPMI 8402) T cells as well as B cells, including Sommer B cells (RPMI 8392). The degree of enrichment of antibody to malignant T cell associated antigens, expressed in terms of the ratios of the amount of globulin bound by T cells relative to the amount bound by B cells, were 6.3 and 5.0 for MOLT 4F and Sommer T cells, respectively.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000941:Antigens / Q000032:analysis*; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D000882:Haplorhini; D006801:Humans; D010215:Papio; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D000941:Antigens