Isolation of normal human IgA, IgM and IgG fragments by polyacrylamide beads immunoadsorbents.
Polyacrylamide beads antibody immunoabsorbents were used in order to isolate human IgA, IgM and fragments of papain-digested human IgG. The proteins obtained were pure as judged by immunochemical techniques. The antisera raised with these purified proteins were monospecific. The binding capacity and the yield were satisfactory. These antibody immunoadsorbents offer several advantages; a) highly purified antigens can be quickly obtained in a one-step procedure from body fluids; b) their handling is easy especially when using a column; c) they can be used for a long period of time and for many experiments without any noticeable loss in their binding capacity.
D000042:Absorption; D000178:Acrylamides; D000888:Antibodies, Anti-Idiotypic; D004406:Dysgammaglobulinemia / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification*; D007071:Immunoglobulin A, Secretory / Q000302:isolation & purification; D007128:Immunoglobulin Fragments / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000302:isolation & purification*; D007137:Immunoglobulin alpha-Chains; D007148:Immunoglobulin mu-Chains; D009101:Multiple Myeloma / Q000276:immunology; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology
D000178:Acrylamides; D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007137:Immunoglobulin alpha-Chains; D007148:Immunoglobulin mu-Chains