[A contribution to formation of artifacts in the autoradiography (author's transl)].
KRAUS and GIESE - as proposed by O'CALLAGHAN, STEVENS and WOOD-- could prevent latent image fading throughout exposure with 89Sr/90 Y by addition of 10 mg KBr and 50 g Glucose per liter of water for flotation of stripping films. This method is not useful for the autoradiography of biological material labelled with tritium. Autoradiograms of rat brain slices were investigated after application 3H-leucine, 3H-methionine, 3H-asparagic-acid and 3H-histidine. By adding the above mentioned salts there was an increase of artifacts and a decrease in reproducibility.
D000596:Amino Acids; D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001417:Background Radiation; D003951:Diagnostic Errors; D004655:Emulsions; D007553:Isotope Labeling; D010781:Photography; D051381:Rats; D012834:Silver; D014316:Tritium
D000596:Amino Acids; D004655:Emulsions; D014316:Tritium; D012834:Silver
Ein Beitrag zur Artefaktbildung in der Autoradiographie