Localization and functional role of the pseudomonas bacteriophage 2 depolymerase.
The adsorption apparatus of phage 2 consits of a symmetrical base plate of snowflake appearance, composed of six droplike spikes 7.0 to 7.5 nm in length with a maximum diameter of 4.5 to 5.0 nm. The spikes are attached by their narrow ends to a central ring 7.0 to 7.5 nm in diameter. Phage 2 deopolymerase, a phage 2-induced hydrolytic enzyme, was found to be a structural protein of phage 2 or in close association with the base plate. Pdp1, a phage 2 mutant, possesses a polypeptide that is antigenically similar to the depolymerase, but devoid of hydrolytic activity. This polypeptide was found to be located in the region of the base plate of pdp1. Treatment of intact cells of strain BI with purified phage 2 depolymerase inhibited the adsorption of phage 2. When phage receptor-containing fractions of slime glycolipoprotein and lipopolysaccharide were hydrolyzed by the depolymerase, amino sugars were released, and the phage-inactivating activities of these fractions were lost. The depolymerase was also observed to induce the lysis of strain BI cells in hypotenic medium. The phage 2 depolymerase appears to play a role in adsorption and release of phage.
D000327:Adsorption; D001426:Bacterial Proteins / Q000378:metabolism; D001435:Bacteriophages / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure; D001665:Binding Sites; D002473:Cell Wall / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000302:isolation & purification; D006867:Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000378:metabolism; D008074:Lipoproteins / Q000378:metabolism; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D014779:Virus Replication
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D008074:Lipoproteins; D011135:Polysaccharides, Bacterial; D006867:Hydrolases; D006026:Glycoside Hydrolases; C019762:bacteriophage 2 depolymerase