[Isopycnic centrifugation in metrizanide gradient of ribosomal ribonucleoprotein precursor particles].
Metrizamide has been described as a new medium for isopycnic centrifugation of unfixed biological particles. In the murine leukemia cell line L 5178 Y, the buoyant density of 80 S and 60 S preribosomal particles was found to be 1,22 g/cm3, which is noticeably lighter than buoyant densities of mature ribosomal particles.
D000818:Animals; D002500:Centrifugation, Isopycnic; D008793:Metrizamide; D051379:Mice; D009698:Nucleoproteins / Q000302:isolation & purification*; D011132:Polyribosomes / Q000032:analysis; D012261:Ribonucleoproteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis*
D009698:Nucleoproteins; D012261:Ribonucleoproteins; D008793:Metrizamide
Centrifugation isopycnique en gradient de métrizamide des particules ribonucléoprotéiques précurseurs des ribosomes