Rapid incorporation of precursors into candicidin by resting cells of Streptomyces griseus.
Labeled acetate, propionate and p-aminobenzoic acid were efficiently incorporated into candicidin by phosphate-limited resting cells of Streptomyces griseus. The efficiency of incorporation in short-term experiments using phosphate-limited resting cells was similar to that achieved previously in long-term experiments using growing cells. (2-14C)Propionate was more efficiently incorpoated than (1-14C)propionate, (U-14C)propionate, or (U-14C)-acetate. p-Aminobenzoic acid incorporation is linear over a 10-hour period while those of acetate or propionate reach a constant level after approximately 4 hours of incubation. Double-labeled candicidin of high specific activity was prepared by supplementing the resting cell system with (3H)acetate and (14C)p-aminobenzoic acid.
D010129:4-Aminobenzoic Acid / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D000935:Antifungal Agents / Q000096:biosynthesis*; D002174:Candicidin / Q000096:biosynthesis*; D007700:Kinetics; D010710:Phosphates / Q000494:pharmacology; D011422:Propionates / Q000378:metabolism; D013305:Streptomyces griseus / Q000378:metabolism*; D013997:Time Factors
D000085:Acetates; D000935:Antifungal Agents; D010710:Phosphates; D011422:Propionates; D002174:Candicidin; D010129:4-Aminobenzoic Acid