[Activity distribution of SDH in liver of mice. A histophotometrical study (author's transl)].
The paper presents histophotometrical measurements of the histochemical SDH reaction with INT as redoxindicator in comparison with biochemical techniques of the determination of SDH activity (with K3[Fe(CN)6] and INT as indicators) and a histochemical method of elution (with INT as indicator). The measurements were performed on liver of mice. For each method absolute values of activity (10(-9) mol substrat/min-mg liver) are calculated as relative values of activity [%]. The standard deviation of the biochemical methods is approximate +/- 4%, of the histochemical methods +/- 8%. The smallest value of detection is 2.5-10(-14) mol formazan of histophotometrical measurement. The correspondence between the measurements of the relative values of activity by the described methods was secured by statistical tests. A statistically significant proportion of activities between periportal zone and central zone like 100:82 was determinated by histophotometrical measurement.
D000818:Animals; D005260:Female; D005292:Ferricyanides; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008815:Mice, Inbred Strains; D013053:Spectrophotometry / Q000379:methods; D013385:Succinate Dehydrogenase / Q000032:analysis*; D013778:Tetrazolium Salts
D005292:Ferricyanides; D013778:Tetrazolium Salts; D013385:Succinate Dehydrogenase
Intralobuläre Enzymaktivitätsverteilung der SDH in der Mäuseleber Eine histophotometrische Studie