title: Induction of dopa (3,4-dihydroxyphenylalanine) decarboxylase in blowfly integument by ecdysone. A demonstration of synthesis of the enzyme de novo.
abstract: The activity of the enzyme dopa (3,4-dihydroxyphenylalanine) decarboxylase, present in the epidermis cells of blowfly larvae, increases during the late third instar under the influence of the steroid hormone, ecdysone. By using the double-labelling technique and immune precipitation with univalent antibody to dopa decarboxylase, we demonstrated that the increase in enzyme activity was due to a stimulation of synthesis of enzyme molecules de novo. In this respect, the action of ecdysone is similar to the action of other steroid hormones.
mesh_terms: D000818:Animals; D004175:Diptera / Q000187:drug effects / Q000201:enzymology*; D004296:Dopa Decarboxylase / Q000096:biosynthesis* / Q000276:immunology; D004440:Ecdysone / Q000494:pharmacology*; D004790:Enzyme Induction; D005779:Immunodiffusion; D007814:Larva / Q000187:drug effects / Q000201:enzymology; D007930:Leucine / Q000378:metabolism
chemical_list: D004440:Ecdysone; D004296:Dopa Decarboxylase; D007930:Leucine