title: Interaction of purified leukocidin from Pseudomonas aeruginosa with Bovine polymorphonuclear leukocytes.
abstract: The interaction of purified leukocidin from Pseudomonas aeruginosa, strain 158, with polymorphonuclear leukocytes of cattle (PMLC) was studied by using 125I-labeled toxin. According to the Scatchard plot, PMLC offered two binding sites for leukocidin: one at the surface of the plasma membrane, and a second one that presumably became accessible to the toxin in the course of the cytotoxic action. Toxin once fixed to PMLC at 37 C could not be detached from the cells by either chemical or mechanical treatment. However, active leukocidin was liberated if it was bound to PMLC at 4 C and the temperature of the cell suspension was subsequently increased to 37 C. In the presence of Ca2+, the velocity of toxin fixation was accelerated and the rate of fixation was increased. Preliminary investigations on the identification of the leukocidin-binding material indicated the leukocidin receptor to be an integral protein of the plasma membrane.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002462:Cell Membrane / Q000276:immunology; D007038:Hypotonic Solutions; D007106:Immune Sera / Q000494:pharmacology; D007956:Leukocidins / Q000378:metabolism* / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014121:Toxoids
chemical_list: D007038:Hypotonic Solutions; D007106:Immune Sera; D007956:Leukocidins; D014121:Toxoids; D012965:Sodium Chloride