title: DNA repair after UV and gamma irradiation. I. Rat spleen cells.
abstract: An in vitro microculture was set up to standardize DNA enzymatic repair in rat spleen lymphocytes after exposure to ultraviolet or gamma rays by measuring the tritiated thymidine uptake. A relation between irradiation dose and DNA repair is always observable when cell viability and scheduled DNA synthesis are taken into account. This micromethod utilizing rat spleen cells appears to be a suitable system for such a study since it permits a satisfactory evaluation of DNA repair.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003584:Cytological Techniques; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects*; D005260:Female; D005720:Gamma Rays; D006918:Hydroxyurea / Q000494:pharmacology; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000378:metabolism / Q000528:radiation effects*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays
chemical_list: D014316:Tritium; D004247:DNA; D013936:Thymidine; D006918:Hydroxyurea