Application of the indirect fluorescent antibody test for diagnosis of Theileria hirci infection of sheep: using cell culture schizont antigen.
The indirect fluorescent antibody test was applied for detection of circulating antibodies in sheep as a result of Theileria hirci infections. A schizont antigen was prepared from an in vitro culture suspension of lymphoid cells infected with T. hirci macroschizonts. The peak antibody titre of 1/8, 192 was reached 24 days after the initial antibody rise in the sheep experimentally infected by means of Hyalomma anatolicum anatolicum ticks.
D000818:Animals; D000941:Antigens; D016782:Apicomplexa / Q000276:immunology*; D002417:Cattle; D005455:Fluorescent Antibody Technique / Q000379:methods*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*; D013801:Theileriasis / Q000175:diagnosis*
D000941:Antigens