[Relationship between beta-lactamase activity of clinical strains of Pseudomonas pyocyanea and their resistance to penicillins].
The study of 50 clinical strains of Ps. aeruginosa revealed their high resistance to benzylpenicillin, ampicillin, oxacillin, streptomycin and kanamycin with a tendency to polyresistance. The same strains were highly sensitive to gentamycin and polymyxin. The strains produced constitutive beta-lactamase in small amounts. The enzyme synthesis in Ps. aeruginosa was induced by benzylpenicillin. Still high concentrations of it were required for the induction. The maximum induction of beta-lactamase synthesis was observed by the 6th hour of the inductor addition. The maximum induction level was observed 4 hours after addition of benzylpenicillin. The level of induction of beta-lactamase synthesis in the strains ranged within 5-93. A significant part of the enzyme was liberated from the cells during induction. Interaction between the induction level of beta-lactamase synthesis and resistance of Ps. aeruginosa to penicillins was found.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010400:Penicillin G / Q000494:pharmacology; D010403:Penicillin Resistance; D010405:Penicillinase / Q000096:biosynthesis / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D010405:Penicillinase; D010400:Penicillin G
Sviaz' mezhdu beta-laktamaznoĭ aktivnost'iu klinicheskikh shtammov sinegnonoĭ palochki i ikh ustoĭchivost'iu k penitsillinam