[Comparison of conventional methods for the determination of albumin, transferrin and ceruloplasmin in serum with immunological reference methods (author's transl)].
Radial immunodiffusion, which is a presently accepted reference method, was compared with the mechanized immunoprecipitation. Each of these specific methods was compared with two normal clinical-chemical methods for the determination of albumin and transferrin, and an enzymic method for the determination of ceruloplasmin. The correlation coefficients, the mean slope for regression, and the percentage variation of the average values from those of the average values of the reference methods are presented. In the determination of albumin and ceruloplasmin, radial immunodiffusion and mechanized immunoprecipitation give the same results. Surprisingly, these two specific methods give different results in the determination of transferrin. In as far as there is no doubt concerning the accuracy of the mechanized immunoprecipitation, this method is recommended, since it also has the advantage of being less demanding in cost and time.
D001322:Autoanalysis; D001797:Blood Protein Electrophoresis; D002570:Ceruloplasmin / Q000032:analysis*; D006801:Humans; D007118:Immunoassay; D005779:Immunodiffusion; D007501:Iron / Q000097:blood; D007504:Iron Radioisotopes; D008722:Methods; D011233:Precipitin Tests; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis*; D014168:Transferrin / Q000032:analysis*
D007504:Iron Radioisotopes; D012709:Serum Albumin; D014168:Transferrin; D007501:Iron; D002570:Ceruloplasmin
Vergleiche konventioneller Methoden zur Albumin-, Transferrin- und Coeruloplasmin-Bestimmung im Serum mit immunologischen Referenzmethoden