Autotransufsion of 3H-cytidine-labelled blood lymphocytes in patients with Hodgkin's disease and non-Hodgkin patients. I. Limitations of the method.
Autotransfusion of 3H-cytidine-labelled blood lymphocytes followed by autoradiographic evaluation as studied in 11 patients with Hodgkin's disease and pool sizes of lymphocytes. However, it is complicated by two major pitfalls; (1) The free (extracellular) activity of oH-cytidine transfused along with the cell-bound label is sufficient to cause DNA labelling of newly produced lymphocytes in the body. (2) The soluble intracellular pool of 3H-labelled compounds causes an increase of labelling intensity of lymphocyte RNA in the first hours after cell transfusion. Methods to correct for these sources of error are described.
D001804:Blood Transfusion, Autologous / Q000379:methods; D003562:Cytidine; D006689:Hodgkin Disease / Q000097:blood / Q000628:therapy*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D009369:Neoplasms / Q000097:blood / Q000628:therapy; D012595:Scleroderma, Systemic / Q000097:blood / Q000628:therapy; D013997:Time Factors
D003562:Cytidine