[Isolation of the mRNA for interferon and its integration into the genome of a foreign host].
The study of superinduction and translation of interferon mRNA (mRNA-IF) in four cell systems was the experimental basis for tests on the establishment in mouse and monkey cells (L-1210 and BSC) of the capacity for long-term production of biologically active chicken interferon after their inoculation with chicken mRNA-IF. The interferon was tested and identified by the species-specificity, thermostability and antigenic specificity. During 3 months (the observation period) after a single inoculation of chicken mRNA-IF the L-1210 and BSC cells in response to induction with poly: C produced the homologous (mouse or monkey) and heterologous (chicken) interferons without any signs of decline of the latter's production. Titres of the heterologous interferon exceeded those of homologous interferon by approximately 10-fold. The dynamics of production of chicken interferon after induction and the sensitivity of this process to actinomycin D permit a conclusion on similar mechanisms of transcription and translation of homologous and heterologous interferons. It is suggested that integration of the genetic information for synthesis of chicken interferon with the genome of heterogenous cells may be a possible mechanism of this phenomenon.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D005347:Fibroblasts; D005815:Genetic Code; D005818:Genetic Engineering; D000882:Haplorhini; D006801:Humans; D007369:Interferon Inducers; D007372:Interferons / Q000096:biosynthesis*; D007939:Leukemia L1210; D012333:RNA, Messenger / Q000008:administration & dosage / Q000302:isolation & purification; D013045:Species Specificity
D007369:Interferon Inducers; D012333:RNA, Messenger; D007372:Interferons
Vydelenie mRNK dlia interferona i ee integratsiia v genom chuzherodnogo khoziaina