[A glucose dehydrogenase for the determination of glucose concentrations in body fluids (author's transl)].
The isolation of glucose dehydrogenase from Bacillus megaterium M 1286 is outlined. Data on the specificity of the enzyme towards carbohydrates are given. A specific method for glucose determination using this enzyme was developed. Methods and results of four variants of this glucose determination are presented: End point determination in the UV range, determination with formazan as reaction product, kinetic determination in the UV range, and continuous flow analysis in the UV range (AutoAnalyzer method).
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification*; D001322:Autoanalysis; D001410:Bacillus megaterium / Q000201:enzymology; D001786:Blood Glucose / Q000032:analysis; D002238:Carbohydrate Epimerases; D003124:Colorimetry; D005947:Glucose / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D008722:Methods; D009247:NADH, NADPH Oxidoreductases; D013056:Spectrophotometry, Ultraviolet
D001786:Blood Glucose; D000429:Alcohol Oxidoreductases; D009247:NADH, NADPH Oxidoreductases; D002238:Carbohydrate Epimerases; D005947:Glucose
Eine Glucose-Dehydrogenase für die Glucose-Bestimmung in Körperflüssigkeiten