Polarographic assay of hydrogen peroxide accumulation in microbial cultures.
A method is described for determining low concentrations of hydrogen peroxide by using a polarographic oxygen electrode to measure the oxygen released into solution on addition of catalase. A sample can be assayed directly without prior manipulation in 3 min. The method is capable of assaying hydrogen peroxide concentrations as low as 7 muM. The method has proved extremely useful for the assay of hydrogen peroxide secreted into milk by lactic acid bacteria.
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006862:Hydrogen Sulfide / Q000096:biosynthesis*; D007773:Lactates / Q000096:biosynthesis; D013294:Lactococcus lactis / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008892:Milk; D010100:Oxygen / Q000032:analysis; D011048:Polarography / Q000379:methods*
D003470:Culture Media; D007773:Lactates; D002374:Catalase; D010100:Oxygen; D006862:Hydrogen Sulfide