Liquid nitrogen cryo-impacting: a new concept for cell disruption.
High-efficiency disruption of bacteria can be accomplished in 2 or more min by the new procedure of liquid nitrogen cryo-impacting. Release of the dipicolinic acid-Ca2+ chelate paralleled the breakage of Bacillus megaterium endospores. Lactate dehydrogenase activity was much better in supernates from liquid nitrogen cryo-impacting-broken Escherichia coli cells than in those from sonically treated and broken E. coli cells.
D001410:Bacillus megaterium / Q000378:metabolism / Q000648:ultrastructure; D001419:Bacteria / Q000648:ultrastructure; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology / Q000648:ultrastructure; D005615:Freezing; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009584:Nitrogen; D010848:Picolinic Acids / Q000378:metabolism; D013010:Sonication; D013171:Spores, Bacterial / Q000378:metabolism / Q000648:ultrastructure; D013295:Streptococcus mutans / Q000378:metabolism / Q000648:ultrastructure
D010848:Picolinic Acids; D007770:L-Lactate Dehydrogenase; D009584:Nitrogen