Separation of human renin substrate from renin and a major contaminating albumin using a concanavalin A-sepharose column.
Human plasma renin substrate was purified and separated from renin by a concanavalin A-Sepharose affinity column. Human renin substrate as well as renin were bound to concanavalin A. Renin substrate was eluted with 0.1 M D-glucose in 20 mM Tris/HCl buffer, pH 8.0. The specific activity increased from 38.5 to 653 ng of angiotensin-I equivalents per mg of protein (17-fold) and the recovery was 85%. Renin was eluted completely with 0.2 M alpha-D-methylglucoside and 0.2 M alpha-D-methylmannoside.
D000804:Angiotensin II / Q000031:analogs & derivatives*; D000808:Angiotensinogen / Q000097:blood / Q000302:isolation & purification*; D002847:Chromatography, Agarose; D003208:Concanavalin A; D006801:Humans; D008722:Methods; D012083:Renin / Q000097:blood / Q000302:isolation & purification*; D012709:Serum Albumin / Q000302:isolation & purification
D012709:Serum Albumin; D000808:Angiotensinogen; D003208:Concanavalin A; D000804:Angiotensin II; D012083:Renin