[Studies on cross reactions of antisera against nucleotid-protein conjugates with denaturated desoxyribonucleic acids].
The reactivity of anti-nucleotide-sera (anti-UMP, anti-AMP, anti-GMP) with denatured DNA's of 10 different sources was investigated by means of quantitative complement fixation. The haptene specificity of the anti-nucleotide antibodies was shown by haptene inhibition of the reaction with DNA. Anti-UMP showed cross-reactions with  other pyrimidine-derivatives, but to a lesser extent; anti-GMP cross-reacted with adenine structures. Quantitative differences were observed in the reaction of anti-UMP, anti-AMP and anti-GMP with DNA's of various (G + C)-contents. These differences could not be correlated in all cases with the overall base composition of the DNA.
D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D003429:Cross Reactions; D004247:DNA / Q000276:immunology*; D006241:Haptens; D007106:Immune Sera; D005779:Immunodiffusion; D009691:Nucleic Acid Denaturation; D011817:Rabbits
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D006241:Haptens; D007106:Immune Sera; D004247:DNA
Untersuchungen zur Kreuzreaktion von Antiseren gegen Nukleotid-Proteinkonjugate mit denaturierten Desoxyribonukleinsäuren