title: Improved tumour yields by means of a TPA-DMBA-TPA variation of the Berenrlum-Mottram experiment on the back skin of NMRI mice. The effect of stationary hyperplasia without inflammation.
abstract: Application of the phorbol ester TPA to the back skin of NMRI mice 14 times within a period of 7 weeks causes a stationary hyperplasia, with a corresponding increase in the labelling index of the basal cells from 1% to 14%. By initiation of skin, which has been pretreated with TPA in this way, with the carcinogen DMBA, followed by continued treatment with TPA (initiation-promotion corresponding to the classical Berenblum-Mottram experiment) the tumour yield (papillomas, carcinomas) is very much higher than that obtained using the scheme of the normal Berenblum-Mottram experiment. The preliminary induction of a stationary hyperplasia with high rates of nucleic acid synthesis must be considered an important co-factor in epidermal carcinogenesis.
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage; D000818:Animals; D001551:Benz(a)Anthracenes / Q000008:administration & dosage; D002277:Carcinoma / Q000139:chemically induced; D005260:Female; D006965:Hyperplasia / Q000139:chemically induced / Q000503:physiopathology*; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000139:chemically induced; D010212:Papilloma / Q000139:chemically induced; D010704:Phorbols / Q000008:administration & dosage; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000139:chemically induced*; D013755:Tetradecanoylphorbol Acetate / Q000008:administration & dosage; D013997:Time Factors
chemical_list: D001551:Benz(a)Anthracenes; D010704:Phorbols; D015127:9,10-Dimethyl-1,2-benzanthracene; D013755:Tetradecanoylphorbol Acetate