Isolation of a DNA-protein complex containing a single DNA fragment which is at or near the replication origin of the Bacillus subtilis chromosome.
A chromosomal fragment containing purA, the nearest marker from the replication origin of the Bacillus subtilis chromosome, was highly purified as a complex containing at least proteins and being solubilized easily during cell lysis. The complex had a markedly higher sedimentation rate (70-120S) than the bulk of the solubilized DNA (40S). The electron microscopic observation showed the complex to be an aggregate of several DNA molecules with a local structure containing amorphous materials which stained black and bushes of RNA. This confirmed biochemical evidences suggesting that the complex is an intermolecular aggregate of the purA-DNA-protein-RNA complex.
D001412:Bacillus subtilis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism*; D003852:Deoxyribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D046911:Macromolecular Substances; D009698:Nucleoproteins / Q000378:metabolism*; D011485:Protein Binding; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D003852:Deoxyribonucleoproteins; D046911:Macromolecular Substances; D009698:Nucleoproteins