[Coagglutination in continuous flow. Techniques and applications].
The principle of coagglutination, the specific agglutination of uncoated red cells by antibody coated ones was applied to the two conventional continuous flow agglutination systems (bromelin-methylcellulose and polybrene-Na-citrate). The technique was used to study a large number of allo- and auto-antibodies of various specificities as well as some drug related antibodies. The sensitivity of the technique proved not to be higher than that of similar systems using free antibodies. It was however shown to be still more economical and elegantly allowed to avoid manual absorption-elution steps prior to investigation of antibody or antigen specificity.
D000918:Antibody Specificity; D001323:Autoantibodies / Q000302:isolation & purification; D001789:Blood Group Antigens; D001963:Bromelains; D002951:Citrates; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012204:Rh-Hr Blood-Group System; D013997:Time Factors
D001323:Autoantibodies; D001789:Blood Group Antigens; D002951:Citrates; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012204:Rh-Hr Blood-Group System; D001963:Bromelains
Coagglutination en flux continu. Techniques et applications