[Histochemical evidence of the aminotransferases. V. Quantitative histochemical investigations of the aminotransferases in organs of the rat (author's transl)].
The quantitative histochemical method was employed the activity of some aminotransferases in the rat organs. Determination was based on extraction of the enzymatic reaction product formazan with alkaline dimethylsulfoxid. Evaluation of the enzymatic activity was given by the formazan quantity related to 1 mug tissue protein. The following transaminases have been studied: aspartate-, alanine-, tyrosine-, phenylalanine-, tryptophane-, glycine-, and serine aminotransferases, as the leucine aminotransferase with substrate leucine, isoleucine and valine. Difference was observed as with respect to one and the same aminotransferase in the examined organs, as well, with respect to the different aminotransferases in one and the same organ.
D000818:Animals; D001921:Brain / Q000201:enzymology; D004121:Dimethyl Sulfoxide; D005562:Formazans; D006651:Histocytochemistry; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009580:Nitroblue Tetrazolium; D051381:Rats; D012972:Sodium Hydroxide; D000637:Transaminases / Q000032:analysis*
D005562:Formazans; D009580:Nitroblue Tetrazolium; D012972:Sodium Hydroxide; D000637:Transaminases; D004121:Dimethyl Sulfoxide
Histochemischer Nachweis der Aminotransferasen. V. Quantitative histochemische Untersuchungen der Aminotransferasenaktivität in Rattenorganen