Sheep erythrocytes binding ability on a mouse renal adenocarcinoma cell line and its hybrid with human lymphocytes.
A mouse renal adenocarcinoma (Rag) cell line, not related to L cells, rosette with erythrocytes from sheep and mice. The Rag-cell rosettes are performed in suspension and on coverslips. Microscopically they rosette like human T cell. No virus-like particles thus far have been found between the rosetted cells. However, the sheep erythrocyte binding ability of Rag cells are very different as compared to human T cells. The former cannot be impaired by trypsin-EDTA treatment nor inhibited by rabbit anti-human thymocyte immunoglobulin, sodium azide or sodium iodoacetate. Two hybrid clones isolated between Rag-human lymphocytes show similar rosette characteristics as the Rag cells.
D000230:Adenocarcinoma / Q000276:immunology*; D000818:Animals; D000961:Antilymphocyte Serum; D001386:Azides / Q000494:pharmacology; D002460:Cell Line; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D066298:In Vitro Techniques; D007461:Iodoacetates / Q000494:pharmacology; D007680:Kidney Neoplasms / Q000276:immunology*; D051379:Mice; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D001386:Azides; D007461:Iodoacetates; D004492:Edetic Acid