Influence of ecdysterone on the growth of cells and synthesis of macromolecules in established cell lines of Drosophila melanogaster.
Ecdysterone (beta-ecdysone) and 2-deoxy-alpha-ecdysone specifically inhibit the growth of established embryonic cells of D. melanogaster. A preparation of alpha-ecdysone is 100 times less active than ecdysterone. The action of ecdysterone is eliminated when it is removed within 24 h, but after 48 h the inhibiting influence of the hormone becomes irreversible. The diploid line, triploid and tetraploid sublines are sensitive to ecdysterone. The diploid subline with a spontaneously arisen translocation of an X-chromosome to an autosome of the third pair is substantially more resistant to ecdysterone. At 24 h after the addition of ecdysterone, there is a 2-5-fold suppression of the synthesis of total RNA; DNA synthesis is lowered to the same degree only after 48 h. The gross synthesis of proteins, measured according to the incorporation of C 14-lysine, was not suppressed even after 48 h.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects* / Q000502:physiology; D002782:Cholestenes / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000254:growth & development; D004441:Ecdysterone / Q000494:pharmacology*; D008239:Lysine / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014178:Translocation, Genetic; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D002782:Cholestenes; D014316:Tritium; D004441:Ecdysterone; D014498:Uracil; D012313:RNA; D004247:DNA; D008239:Lysine; D013936:Thymidine