[Experiences with the glyoxylic-acid fluorescence method for the demonstration of biogenic amines in cuticle preparations, cryostat- and simple frozen sections].
1. Our studies aimed at methodical simplification to make available the glyoxylic acid-fluorescence method to everybody. We succeeded in receiving adequate results in the case of whole mounts, cryostat- and simple frozen sections. 2. Making ready the slide preparations may be divided into 5 steps:--incubation of fresh tissue in glyoxylic acid of 1...2% (pH-value 7.0)--making ready whole mounts, cryostat-or simple frozen sections--air-drying--exposure to 80...100 degrees C for 10...3 minutes--embedding with paraffinum subliquidum. Fluorescence microscopy and photography may be done in the bright and dark field, as well with usual filter-combinations.
D000818:Animals; D001369:Axons / Q000502:physiology; D001679:Biogenic Amines / Q000378:metabolism*; D003584:Cytological Techniques; D005615:Freezing; D006038:Glyoxylates; D006168:Guinea Pigs; D008856:Microscopy, Fluorescence / Q000379:methods; D009420:Nervous System / Q000166:cytology*; D011817:Rabbits; D051381:Rats
D001679:Biogenic Amines; D006038:Glyoxylates
Erfahrungen mit der Glyoxylsäure-Fluoreszenzmethode zum Nachweis biogener Amine in Häutchenpräparaten, Kryostatschnitten und einfachen Gefrierschnitten.