[Sorbent for purification of diamine oxidase by the method of affinity chromatography].
A substrate of diamine oxidase hexamethylene diamine was covalently bound through adipinic acid dihydrazide to Sepharose 4B in order to prepare a sorbent for the purification of diamine oxidase by means of biospecific (affinity) chromatography. A method  was developed to purify diamine oxidase from pig kidney cortex using the sorbent. The method, comprising three steps, yielded enzyme preparations with specific activity 2 000-fold higher than that of kidney homogenate.
D000272:Adipates / Q000494:pharmacology; D006631:Amine Oxidase (Copper-Containing) / Q000302:isolation & purification*; D000818:Animals; D002846:Chromatography, Affinity; D007672:Kidney Cortex / Q000201:enzymology; D011134:Polysaccharides / Q000494:pharmacology*; D012685:Sepharose / Q000494:pharmacology*; D013552:Swine
D000272:Adipates; D011134:Polysaccharides; D012685:Sepharose; D006631:Amine Oxidase (Copper-Containing)
Sorbent dlia ochistki diaminooksidazy metodom affinnoĭ khromatografii