The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Identification of the products from partial degradation of the molecule and derivation of the complete sequence.
1. A series of large oligonucleotide fragments derived from tRNA Cys, were separated chromatographically and the sequence of each was deduced by examination of the products of digestion with pancreatic and T1 ribonucleases. 2. The location of the specific cleavage points in the nucleotide chain was similar to that produced by brief treatment with pancreatic ribonuclease. 3. The fragments could be arranged into two alternative sequences. The correct sequence was deduced by the sequential removal and identification of the first nine nucleotides from the 3'-end of the terminal half of the molecules.
D001483:Base Sequence; D002848:Chromatography, DEAE-Cellulose; D003545:Cysteine; D010179:Pancreas / Q000201:enzymology; D012343:RNA, Transfer / Q000032:analysis*; D006163:Ribonuclease T1; D012260:Ribonucleases; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D012343:RNA, Transfer; D012260:Ribonucleases; D006163:Ribonuclease T1; D003545:Cysteine