[Residues of chloramphenicol in the calf organism following parenteral administration of the Czechoslovak preparation Chronicin inj].
Using two biological methods the chloramphenicol content in various tissues of 11 calves aged 3--18 days was determined after a single-dose therapeutical administration of the Czechoslovak preparation of Chronicin inj. in  the quantity of 12 mg kg-1 body mass. Of four test strains, solely Micrococcus flavus (ATCC 10240) strain proved suitable. Chloramphenicol was quantitatively determined by reading from transformed standard curves. An evalua tion of the two approximately equally sensitive methods yielded the following results: three days after the injection all calves were positive, at 5 and 10 days, one of three calves in each group was negativ similarly to both calves slaughtered 15 days post injection. The residues appeared most frequently in the liver, at the site of injection, and in the fore and hind musculature. Maximum detectable concentrations were recorded in the liver. Culinary and technological treatment of positive meat and liver samples revealed that 15 minutes' boiling and 30 minutes' roasting destroyed the microbiologically active chloramphenicol residues in the samples. Neither deep freezing nor salting was sufficient for residue inactivation, although in some samples a decrease in biological chloramphenicol activity by circa 20--30% was recorded. a preslaughter withdrawal time of 15 days is recommended.
D000003:Abattoirs; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy; D002701:Chloramphenicol / Q000008:administration & dosage / Q000378:metabolism* / Q000627:therapeutic use; D005506:Food Contamination / Q000032:analysis; D007273:Injections, Intramuscular / Q000662:veterinary; D008099:Liver / Q000378:metabolism; D008460:Meat / Q000032:analysis
D002701:Chloramphenicol
Rezidua chloramfenikolu v organismu telat po parenterální aplikaci cs. prípravku Chronicin inj