Detection and preparation of immunoglobulin (Ig) mu chain protein in sera of patients with heavy chain diseases (HCD).
The detection of Igmu chain proteins in the serum of a patient with HCD by a combining of starch gel electrophoresis and immunodiffusion is described. Partial isolation of mu chain protein by immunoadsorbtion on polyacrylamide gel has been acchieved. As no contaminations of mu chain protein with IgM protein could be detected a separate measurement of these proteins using immunoadsorbants of suitable exclusion limits seems to be possible.
D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D006362:Heavy Chain Disease / Q000276:immunology*; D006689:Hodgkin Disease / Q000276:immunology; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000032:analysis*; D007148:Immunoglobulin mu-Chains / Q000032:analysis* / Q000302:isolation & purification
D007143:Immunoglobulin Heavy Chains; D007148:Immunoglobulin mu-Chains