[Immunocytological characterization of cells recognizing antigen and producing antibody during immunization to oxazolone and E. coli lipopolysaccharide].
A preferential cell mediated response is obtained after stimulation with oxazolone () and a humoral response is seen after E. coli lipopolysaccharide (LPS) immunization (). Thus a cytological study of selectively isolated antigen recognizing cells which form rosettes and antibody producing cells which form hemolytic plages has been performed during oxazolone and lipopolysaccharide stimulations. This investigation shows quantitative and qualitative differences of these immunocyte subpopulations in the two stimulations. First the antigen recognizing cells antibody producing cells ratio is fundamentally much higher at all the stages of the oxazolone stimulation than after lipopolysaccharide immunization. On the other hand among these immunocyte subpopulations, T and B cells are identified by electron microscopy in the oxazolone immune reaction, while only B cells are observed after LPS immunization.
D000818:Animals; D000921:Antibody-Producing Cells; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D004926:Escherichia coli; D008070:Lipopolysaccharides / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D010080:Oxazoles / Q000276:immunology*; D010081:Oxazolone / Q000276:immunology*; D011135:Polysaccharides, Bacterial; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D008070:Lipopolysaccharides; D010080:Oxazoles; D011135:Polysaccharides, Bacterial; D011947:Receptors, Antigen, B-Cell; D010081:Oxazolone
Caractérisation immuno-cytologique de cellules reconnaissant l'antigène et produisant des anticorps au cours d'immunisation à l'oxazolone et au lipopolysaccharide d'E. coli