Electrofocusing followed by gradient electrophoresis: a two-dimensional polyacrylamide gel technique for the separation of proteins and its application to the immunoglobulins.
A two-dimensional polyacrylamide gel technique for the separation of proteins is described. Electrofocusing in a 3.5% polyacrylamide gel is followed by electrophoresis through a 4-24% concave polyacrylamide gel gradient. The method resolves a large number of serum proteins and produces highly reproducible patterns. It is particularly useful for studies involving proteins of high molecular weight, such as alpha2-macroglobulin, beta-lipoprotein and IgM. The application of the technique to the immunoglobulins in pathological sera is reported.
D001798:Blood Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D007525:Isoelectric Focusing; D008074:Lipoproteins / Q000097:blood; D008103:Liver Cirrhosis / Q000097:blood; D009101:Multiple Myeloma / Q000097:blood; D009194:Myeloma Proteins / Q000032:analysis; D008258:Waldenstrom Macroglobulinemia / Q000097:blood
D001798:Blood Proteins; D007136:Immunoglobulins; D008074:Lipoproteins; D009194:Myeloma Proteins