Radioelectroimmunoassay of anti-D immunoglobulin on agarose plates containing whole red cells.
By incorporating whole Rh(+) red cells in an agarose plate and adding a small amount of purified 125I anti-D immunoglobulin as a tracer to standards and samples, a radioelectroimmunoassay (REIA) of anti-D immunoglobulin was possible. The results by this method agree with those obtained by the direct method. The coefficient of variation (within plate precision) is less than 9% and the reproducibility is 12% (coefficient of variation for a plasma sample assayed on six occasions during 1 month). The International anti-D serum was compared with the WHO standard preparation 68/419 on five occasions and found to contain 11; 11; 10; 9 and 11 mug per ampoule.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D001666:Binding Sites, Antibody; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004912:Erythrocytes; D006801:Humans; D007552:Isotonic Solutions; D011863:Radioimmunoassay / Q000379:methods; D012204:Rh-Hr Blood-Group System
D000888:Antibodies, Anti-Idiotypic; D007552:Isotonic Solutions; D012204:Rh-Hr Blood-Group System