[Purification of cholinesterase from human serum by affinity chromatography].
The human serum butyrylcholinesterase was rapidly purified with an affinity technique on meta-aminophenyltrimethylammonium-agarose; the ionic strength was 0.25 and the specific elution was achieved with 9-amino-10-methylacridinium. After ion exchange on DEAE-cellulose and molecular filtration, an enzyme purified more than ten thousand times was obtained.
D002091:Butyrylcholinesterase / Q000097:blood / Q000302:isolation & purification*; D002802:Cholinesterases / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D002848:Chromatography, DEAE-Cellulose; D006801:Humans
D002091:Butyrylcholinesterase; D002802:Cholinesterases
Purification de la cholinestérase du sérum humain par affinité