Heat-induced requirements for sucrose or magnesium for expression of heat resistance in Bacillus cereus forespores.
The addition of 0.6 M sucrose of 0.016 M Mg2+ to the enumeration medium was required for early expression of heat resistance (10 min at 70 degrees C) in stage V Bacillus cereus forspores. The addition of Mg2+ to the sporulation medium did not remove this requirment for sucrose of Mg2+. The heat damage did not affect forespore germination or outgrowth, but injured cells in the absence of sucrose or Mg2+ were not capable of cell division. The heat-induced sublethal damage apparently affected the forspore component(s) that could be repaired or was capable of normal function in the presence of added Mg2+ or sucrose.
D001409:Bacillus cereus / Q000502:physiology*; D003470:Culture Media; D006358:Hot Temperature; D008274:Magnesium / Q000502:physiology*; D013171:Spores, Bacterial; D013395:Sucrose / Q000502:physiology*; D013997:Time Factors
D003470:Culture Media; D013395:Sucrose; D008274:Magnesium