[2 interconvertible forms of L type pyruvate kinase from the rabbit adrenal cortex].
Pyruvate kinase (PK-II) isoenzyme from rabbit adrenal cortex was isolated by chromatography on DEAE-cellulose during long-term incubation in the cold. The enzyme is resistant to fructose-1,6-diphosphate (FDP) and L-alanine. Dithiotreitol and preincubation for 10 min. at 30 degrees C did not change the resistance of the enzyme to these factors. Preincubation for 30 min. at 30 degrees C in the presence of EDTA (3.3 mM) converted the enzyme into the FDP-sensitive form, the dependency curve of the initial reaction rate on the concentration of phosphoenolpyruvate being S-form. Sucrose and dithiotreitol contributed the isolation of PK-II from rabbit adrenal cortex in the form having a higher degree of the interaction of active sites.
D000302:Adrenal Cortex / Q000201:enzymology*; D000311:Adrenal Glands / Q000201:enzymology*; D000409:Alanine / Q000494:pharmacology; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D004492:Edetic Acid / Q000494:pharmacology; D005636:Fructosephosphates / Q000494:pharmacology; D007527:Isoenzymes / Q000302:isolation & purification*; D010728:Phosphoenolpyruvate / Q000494:pharmacology; D011770:Pyruvate Kinase / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits
D005636:Fructosephosphates; D007527:Isoenzymes; D010728:Phosphoenolpyruvate; D004492:Edetic Acid; D011770:Pyruvate Kinase; D000409:Alanine
Dve vzaimoprevrashchaiushchiesia formy piruvatkinazy L-tipa iz kory pochki krolika