Purification and characterization of tRNAMet-f, tRNAPhe and tRNATyr2 from Baccillus subtilis.
Three tRNAs specific for methionine, phenylalanine and tyrosine were isolated from the total tRNA of Bacillus subtilis by chromatographic procedures using BD-cellulose and reversed-phase (5) chromatography. The acceptor activities of the purified tRNAs are 1160, 1260 and 1320 pmoles per A260nm unit for tRNAMetf, tRNAPhe and tRNATyr2 respectively. In tRNAMetf and tRNAPhe ribothymidine, pseudouridine and dihydrouridine are present, in addition, in tRNAPhe 7-methyguanosine and a 2'-O-methylated nucleoside were found. The modified nucleosides of tRNATyr2 are ribothymidine, pseudouridine, dihydrouridine, 4-thiouridine and 1-methyladenosine. The results suggest the presence of 2-methylthio-N6(delta 2-isopentenyl)adenosine in tRNAPhe and tRNATyr2. The thermal denaturation profiles of the three tRAN species are presented.
D001412:Bacillus subtilis / Q000032:analysis*; D006358:Hot Temperature; D008274:Magnesium; D009239:N-Formylmethionine; D009691:Nucleic Acid Denaturation; D010649:Phenylalanine; D012343:RNA, Transfer / Q000302:isolation & purification / Q000378:metabolism; D012265:Ribonucleotides / Q000032:analysis; D014443:Tyrosine
D012265:Ribonucleotides; D009239:N-Formylmethionine; D014443:Tyrosine; D010649:Phenylalanine; D012343:RNA, Transfer; D008274:Magnesium