Role of vitamin B12 in the reduction of ribonucleotides into deoxyribonucleotides in Drosophila cells grown in vitro.
Adenosine was found to inhibit growth of Drosophila melanogaster cells in culture. This toxic effect is prevented by the addition of uridine + deoxyuridine, or uridine + deoxycytidine. In the presence of vitamin B12, uridine alone is sufficient to sustain proliferation of Drosophila cells inhibited by adenosine. Moreover, vitamin B12 increases the incorporation of [3H] uridine into DNA, and decreases the incorporation of [3H] thymidine. No modification of the incorporation of [14C] adenine, [14C] adenonsine or [3H] cytosine into DNA could be found in the presence of vitamin B12. It is concluded that vitamin B12 is involved in an enhanced conversion of uridine ribonucleotides into deoxyribonucleotides derived from uridine.
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D003854:Deoxyribonucleotides / Q000096:biosynthesis*; D004331:Drosophila melanogaster; D004789:Enzyme Activation; D012264:Ribonucleotide Reductases / Q000378:metabolism; D014529:Uridine / Q000378:metabolism / Q000494:pharmacology; D014805:Vitamin B 12 / Q000494:pharmacology*
D003854:Deoxyribonucleotides; D004247:DNA; D012264:Ribonucleotide Reductases; D000241:Adenosine; D014805:Vitamin B 12; D014529:Uridine