De-esterification of cephalosporin para-nitrobenzyl esters by microbial enzymes.
Bacteria and actinomycetes were screened for esterase enzymes capable of removing the para-nitrobenzyl ester from cephalosporins. An esterase preparation from Bacillus subtilis was used to prepare cephalexin and 7-ADCA from the corresponding para-nitrobenzyl esters.
D000190:Actinomyces / Q000201:enzymology; D001412:Bacillus subtilis / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology*; D001593:Benzyl Compounds / Q000378:metabolism*; D002506:Cephalexin / Q000302:isolation & purification; D002511:Cephalosporins / Q000302:isolation & purification / Q000378:metabolism*; D004950:Esterases / Q000302:isolation & purification / Q000378:metabolism*; D004951:Esterification
D001593:Benzyl Compounds; D002511:Cephalosporins; D004950:Esterases; D002506:Cephalexin