Appearance of cytolytic antibodies in sheep lymph following immunisation with tumour cells. Identification of antibody subclasses.
Sheep were immunised with mouse P815 tumour cell suspensions and at intervals afterwards lymph was collected draining from the stimulated lymph nodes. The lymph samples were fractionated by column chromatography into IgM, IgG1 and IgG2 antibody fractions; these were then assayed for cytolytic functions on 51Cr labelled target P815 cells. Complement dependent antibodies were assayed using sheep complement; activity was first detected in the IgM fraction 3-4 days after immunisation and in the IgG1 fraction at 5-6 days. No CDA activity was found in fractions of the IgG2 antibody subclass at any time. Leucocyte dependent antibodies were detected only in the IgG fraction, and they appeared simultaneously in both IgG1 and IgG2 subclasses 5-6 days after immunisation.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis / Q000096:biosynthesis*; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis; D007962:Leukocytes / Q000276:immunology; D008196:Lymph / Q000276:immunology*; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D012756:Sheep / Q000276:immunology*
D000912:Antibodies, Neoplasm; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003165:Complement System Proteins