The induction of mutants of Acinetobacter calcoaceticus NCIB8250 and their selection by vancomycin.
The mutagenic and lethal effects of N-methyl-N'-nitro-N-nitrosoguanidine (NTG), ethyl methanesulphonate (EMS), ultraviolet light irradiation and near-ultraviolet light irradiation with 8-methoxypsoralen on the bacterium Acinetobacter calcoaceticus NCIB8250 were examined. The production of auxotrophic mutants was used as a measure of mutagenic efficiency. Under appropriate conditions all four agents were mutagenic. EMS and NTG although more effective than irradiation, did not cause such a high frequency of mutation as has been observed with other bacteria. A combination of vancomycin and penicillin V gave enrichment of non-metabolizing bacteria and optimum conditions were found for the use of these compounds in a selection technique.
D000150:Acinetobacter / Q000187:drug effects* / Q000302:isolation & purification* / Q000378:metabolism / Q000528:radiation effects; D001592:Benzyl Alcohols / Q000378:metabolism; D002228:Carbenicillin / Q000494:pharmacology; D003523:Cycloserine / Q000494:pharmacology; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005971:Glutamates / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D008698:Mesylates; D008730:Methoxsalen / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens; D009154:Mutation; D010400:Penicillin G / Q000494:pharmacology; D010403:Penicillin Resistance; D010404:Penicillin V / Q000494:pharmacology; D010648:Phenylacetates / Q000378:metabolism; D011830:Radiation Effects; D013045:Species Specificity; D013386:Succinates / Q000378:metabolism; D014466:Ultraviolet Rays; D014640:Vancomycin / Q000494:pharmacology*
D001592:Benzyl Alcohols; D005971:Glutamates; D008293:Malates; D008698:Mesylates; D009153:Mutagens; D010648:Phenylacetates; D013386:Succinates; D008769:Methylnitronitrosoguanidine; D014640:Vancomycin; D003523:Cycloserine; D005020:Ethyl Methanesulfonate; D002228:Carbenicillin; D010400:Penicillin G; D008730:Methoxsalen; D010404:Penicillin V