Quantitation of subclasses of mononuclear cells in normal human blood by membrane receptor studies.
A technique for quantitating mononuclear cells expressing E, EA(IgG), EAC receptors, immunoglobulin carrying cells and phagocytic cells in normal human venous blood is described; normal values for each of these classes of mononuclear cell are established. The effects of different methods of cell preparation have been considered; these were found not to have a significant effect on the final values. In addition, we describe a second category of 'fluorescent non-capping' cells and show that these are numerically equivalent to cells which ingest neutral red.
D000328:Adult; D000818:Animals; D001667:Binding, Competitive; D001799:Blood Sedimentation; D002462:Cell Membrane / Q000276:immunology; D002469:Cell Separation; D002470:Cell Survival; D003165:Complement System Proteins; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006801:Humans; D007104:Immune Adherence Reaction; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D010586:Phagocytes; D011947:Receptors, Antigen, B-Cell; D011955:Receptors, Drug; D012756:Sheep
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011947:Receptors, Antigen, B-Cell; D011955:Receptors, Drug; D003165:Complement System Proteins; D004492:Edetic Acid