title: Comparison of four culture media for the isolation of Mycobacterium tuberculosis: a 2-year study.
abstract: Four media, Lowenstein-Jensen, Middlebrook, Petragnani, and ribonucleic acid, were tested for comparative ability to detect Mycobacterium tuberculosis. Specimens used included sputum, urine, tissue, and gastric washings. Three types of comparison were used: (i) comparison derived from randomized specimens; (ii) comparison of cultures from newly diagnosed cases that had received no prior therapy; and (iii) comparison of cultures from specimens whose initial direct smears were negative. Overall, ribonucleic acid medium performed best, but the differences among the four media were small.
mesh_terms: D002555:Cerebrospinal Fluid / Q000382:microbiology; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005750:Gastric Juice / Q000382:microbiology; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D012313:RNA; D013183:Sputum / Q000382:microbiology; D014376:Tuberculosis / Q000188:drug therapy / Q000382:microbiology; D014556:Urine / Q000382:microbiology
chemical_list: D003470:Culture Media; D012313:RNA