[Factors regulating protein synthesis by rabbit reticulocyte ribosomes: variations in their concentrations during cell maturation].
An activator as well as an inhibitor of protein synthesis, were isolated from rabbit reticulocyte ribosomes. The ribosomes of the erythrocytes are inactive in protein synthesis and lacked the activator. However, when the activator moiety was added to the erythrocyte ribosomes, there was a significant increase in their capacity for protein synthesis, provided these ribosomes were freed of their inhibitor by washing beforehand with a detergent.
D000818:Animals; D002454:Cell Differentiation; D002474:Cell-Free System; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000378:metabolism; D003864:Depression, Chemical; D003902:Detergents / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism* / Q000648:ultrastructure; D007930:Leucine / Q000378:metabolism; D008297:Male; D010649:Phenylalanine / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D014176:Protein Biosynthesis; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism* / Q000648:ultrastructure; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013268:Stimulation, Chemical
D003902:Detergents; D011072:Poly U; D010649:Phenylalanine; D011189:Potassium Chloride; D007930:Leucine
Etude des facteurs intervenant dans la synthèse protéique par les ribosomes de réticulocytes de Lapin: variations de leur taux au cours de la maturation cellulaire