The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor.
A quantitative assay that measures fibroblast chemotaxis in vitro is described. Application of this technique has revealed that peripheral blood lymphocytes stimulated by antigen or mitogen in vitro produce a factor that is chemotactic for human dermal fibroblasts. This lymphocyte-derived chemotactic factor for fibroblasts (LDCF-F) is different from the lymphokine that is chemotactic for monocytes or macrophages. Macrophages are required for the generation of LDCF-F by T lymphocytes stimulated by phytohemagglutinin. The fibroblast chemotactic factor is heat stable (56 degrees C for 30 min), trypsin sensitive, and neuraminidase resistant. LDCF-F could function to attact connective tissue fibroblasts to sites at which cell-mediated immune reactions are occurring in vivo.
D002460:Cell Line; D002633:Chemotaxis; D004492:Edetic Acid / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology*; D008222:Lymphokines / Q000032:analysis / Q000502:physiology*; D008264:Macrophages / Q000502:physiology; D009439:Neuraminidase; D014357:Trypsin
D008222:Lymphokines; D004492:Edetic Acid; D009439:Neuraminidase; D014357:Trypsin