[Alkaline protease production].
The influence of culture medium composition on alkaline protease production was studies. Various carbon sources such as glycerol, glucose, lactose and starch were tested. Different concentrations of lactose and bactopeptone have been tested. The optimum medium composition was found to be: (g/l) lactose 20.0; bactopeptne Difco 10.0; NaCl 1.5;mgSO47.H20 0.15; CaCl2 0.06; K2HPO4 1.5; KH2PO4 1.5; Na2SO4 1.5; MnCL2.4H2O 0.01. A cell concentration of c.a 9.5 g/l has been obtained after 30 hours. The maximun alkaline protease production (5,000 uAPAM/ml) was attained after 60 hours. The trials were carried on rotary shaker in 1 liter erlenmeyer flasks containing 250 ml of medium.
D001412:Bacillus subtilis / Q000254:growth & development*; D050260:Carbohydrate Metabolism; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D013213:Starch / Q000378:metabolism
D003470:Culture Media; D013213:Starch; D010447:Peptide Hydrolases; D005947:Glucose; D007785:Lactose; D005990:Glycerol
Estudios sobre obtención de proteasas alcalinas