Evidence for the involvement of the Ss protein of the mouse in the hemolytic complement system.
A significant within-strain correlation has been demonstrated between the levels of Ss and hemolytic complement (C) activity in two Ss-high strains. Mouse serum specifically depleted of Ss by absorption with F(ab')2 fragments of anti-Ss had negligible C activity. In control experiments, Ss-specific antigen-antibody complexes formed with F(ab')2 fragments did not fix rabbit C, and bovine serum albumin-specific antigen-antibody complexes formed with F(ab')2 fragments did not fix mouse C. Therefore the removal of C activity by anti-Ss [F(ab')2] was apparently not due to C fixation. These results suggest that the Ss protein is a necessary component of the C system.
D000818:Animals; D001798:Blood Proteins; D003168:Complement Fixation Tests; D003165:Complement System Proteins; D006461:Hemolysis; D007106:Immune Sera; D005779:Immunodiffusion; D007125:Immunogenetics; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine
D001798:Blood Proteins; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D003165:Complement System Proteins