[Antiglycogen activity of rabbit antisera raised against a strain of Escherichia coli 013].
Rabbit antisera raised against a strain of E. coli 013 (Su 4321/41 013 K11 H11) contain high titres of antiglycogen antibodies. This activity was evidenced through positive immunofluorescent reactions observed on liver and muscle sections. Such reactions disappeared when the sections were previously treated with alpha-amylase. They were inhibited with Oyster glycogen, phenolalcohol, perchloric acid, and trichloroacetic liver extracts. These same substances had no inhibiting activity when treated with alpha-amylase. The immunofluorescent reactions were negative on livers from Mice treated with endotoxins and insulin which are known to deplete the stores of liver glycogen. The antisera gave interfacial precipitates with glycogen and liver extracts. The same substances, as well as the LPS from E. coli 013, had a specific inhibiting activity in a radioimmunoassay test using glycogen linked to bovine serumalbumine labeled with 125I.
D000818:Animals; D000907:Antibodies, Bacterial; D000937:Antigen-Antibody Reactions; D004926:Escherichia coli / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006003:Glycogen / Q000276:immunology*; D007118:Immunoassay; D007700:Kinetics; D011817:Rabbits / Q000276:immunology; D012475:Salmonella / Q000276:immunology; D013045:Species Specificity
D000907:Antibodies, Bacterial; D006003:Glycogen
Activité antiglycogène de sérums de lapins immunisés avec une souche d'Escherichia coli 013