An inhibitor to erythrocyte agglutination in bovine albumin preparations.
Three out of 28 commercial preparations of bovine serum albumin have been encountered which have an inhibitory effect on the assay of anti-Rh-o(D) using the Technicon AutoAnalyser. The inhibitory property, which can also be demonstrated by standard manual serological techniques, appears to be directed towards the second stage of the agglutination reaction. An automated screening procedure for bovine serum albumin preparations and some properties of the inhibitor are described.
D000042:Absorption; D001322:Autoanalysis; D001386:Azides / Q000032:analysis; D001793:Blood Preservation; D001963:Bromelains; D004589:Electrophoresis, Disc; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D006384:Hemagglutination / Q000187:drug effects*; D006801:Humans; D007106:Immune Sera; D007202:Indicators and Reagents; D008297:Male; D008747:Methylcellulose; D012204:Rh-Hr Blood-Group System / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013431:Sulfates / Q000032:analysis; D014462:Ultrafiltration
D001386:Azides; D007106:Immune Sera; D007202:Indicators and Reagents; D012204:Rh-Hr Blood-Group System; D013431:Sulfates; D012710:Serum Albumin, Bovine; D001963:Bromelains; D008747:Methylcellulose