[Study of the mechanism of adsorption and activation of enzymes of the contact phase of blood coagulation].
Desorption of enzymes, participating in contact phase of blood coagulation, from silica surface was studied under effect of increased ion strength, in presence of urea, EDTA and surface-active substances. All these agents varied in their capacity to decrease the enzymes activity and to cause the desorption of contact factors. Ionic and hydrogen bonds were shown to carry out the leading role in adsorption of factor XII and in the initiation of the contact phase of blood coagulation.
D000327:Adsorption; D000925:Anticoagulants / Q000494:pharmacology; D001777:Blood Coagulation; D003902:Detergents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004950:Esterases / Q000097:blood*; D005174:Factor XII; D005898:Glass; D006801:Humans; D066298:In Vitro Techniques; D011189:Potassium Chloride / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*; D014508:Urea / Q000494:pharmacology; D014867:Water
D000925:Anticoagulants; D003902:Detergents; D013501:Surface-Active Agents; D014867:Water; D012967:Sodium Dodecyl Sulfate; D011189:Potassium Chloride; D014508:Urea; D005174:Factor XII; D004492:Edetic Acid; D012964:Sodium; D004950:Esterases
K izucheniiu mekhanizma adsorptsii i aktivatsii fermentov kontaktnoĭ fazy svertyvaniia krovi