Application of the SIP technique to the determination of proteins in the cerebrospinal fluid and serum.
The application of the Surface Immune Precipitation (SIP) technique to the determination of proteins in cerebrospinal fluid and serum is described. SIP measurements of CSF total protein and albumin exhibited good linear correlation with the sulfosalicylic acid turbidimetric method for total protein and the radial immunodiffusion (RID) method for albumin. A coefficient of variation of 20% for total protein and 11% for albumin was obtained on clinical CSF specimens. Clinical serum samples were analyzed for C3, alpha1 acid glycoprotein, and alpha1 antitrypsin by both the SIP and RID methods. Good linear correlation was obtained between the two systems with a coefficient of variation of 11, 5, and 6% for the C3, alpha1 acid glycoprotein, and alpha1 antitrypsin systems, respectively.
D000418:Albumins / Q000134:cerebrospinal fluid; D001798:Blood Proteins / Q000032:analysis*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D011232:Chemical Precipitation; D003176:Complement C3 / Q000032:analysis; D005782:Gels; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D005779:Immunodiffusion; D009391:Nephelometry and Turbidimetry; D012685:Sepharose; D000515:alpha 1-Antitrypsin / Q000032:analysis
D000418:Albumins; D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins; D003176:Complement C3; D005782:Gels; D006023:Glycoproteins; D000515:alpha 1-Antitrypsin; D012685:Sepharose