title: Serological diagnosis of petriellidosis (allescheriosis). I. Isolation and characterization of soluble antigens from Allescheria boydu and Monosporium apiospermum.
abstract: Soluble antigens in culture filtrates of three strains of Petriellidium boydii and three strains of Monosporium apiospermum were examined. Antigens were separated from concentrated crude filtrates by anion-exchange chromatography. A single major peak (Antigen 1), constituting a significant proportion of the total recoverable carbohydrate, was the only product isolated from each of four chromatographed filtrates. Depending on the fungus strain, Antigen 1 consisted of 90--96% carbohydrate, 3-4% protein, and 2-4% nucleic acid. Antigen 1 was found to consist of a population of molecules with a heterogeneous molecular size when assayed by gel filtration chromatography; however, isolated fractions of Antigen 1 proved to be immunologically identical when examined by Ouchterlony immunodiffusion. In addition, Antigen 1 from each strain was immunologically identical to similar preparations of Antigen 1 from the other five fungus strains. Chromatography of culture filtrates from two strains of M. apiospermum revealed a second peak (Antigen 2), which was found to consist of 70% carbohydrate, 16% protein, and 4% nucleic acid. Although Antigen 2 contained four times as much protein as Antigen 1, the two preparations were immunologically identical by immunodiffusion tests. Ion-exchange chromatography proved to be a useful procedure for isolating antigens of P. boydii and M. apiospermum from culture filtrates.
mesh_terms: D000946:Antigens, Fungal / Q000032:analysis / Q000302:isolation & purification; D001203:Ascomycota / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D003937:Diagnosis, Differential; D005656:Fungal Proteins / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D009181:Mycoses / Q000175:diagnosis*; D009696:Nucleic Acids / Q000032:analysis; D011541:Pseudallescheria / Q000276:immunology*; D012698:Serologic Tests; D012995:Solubility; D013045:Species Specificity
chemical_list: D000946:Antigens, Fungal; D002241:Carbohydrates; D005656:Fungal Proteins; D009696:Nucleic Acids