Assignment of a human gene for tryptophanyl-tRNA synthetase to chromosome 14 using human-mouse somatic cell hybrids.
Human tryptophanyl-tRNA SYNTHETASE (Trp-RS, EC 6.1.1.2) can be separated from its mouse counterpart by Cellogel electrophoresis. Analysis of the presence or absence of human Trp-RS and other human enzyme markers in eleven independently dervied cell lines of human-mouse somatic cell hybrids revealed that the expression of Trp-RS is correlated with the expression of human nucleoside phosphorylase (NP, EC 2.4.2.1). The syntenic  relationship between Trp-RS and NP permits the assignment of the structural gene for Trp-RS to human chromosome 14. Karyotype and isozyme analysis of these hybrid clones rules out other linkage assignments.
D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis*; D000818:Animals; D002874:Chromosome Mapping; D002901:Chromosomes, Human, 13-15; D005796:Genes; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure; D066298:In Vitro Techniques; D051379:Mice; D010641:Phenotype; D011683:Purine-Nucleoside Phosphorylase / Q000096:biosynthesis; D014369:Tryptophan-tRNA Ligase / Q000096:biosynthesis*
D011683:Purine-Nucleoside Phosphorylase; D000604:Amino Acyl-tRNA Synthetases; D014369:Tryptophan-tRNA Ligase