Thermal denaturation of B. subtilis DNA in H2O and D2O observed by electron microscopy.
In the present work we studied the initial part of thermal denaturation curves of B. subtilis DNA in normal and heavy water, observing, by electron microscopy, the "opening" of DNA as a function of temperature. The results support the hypothesis that D(2)O plays a stabilizing role on strands separation during thermal denaturation of DNA.
D001412:Bacillus subtilis / Q000032:analysis*; D001665:Binding Sites; D004269:DNA, Bacterial / Q000302:isolation & purification; D003903:Deuterium; D006358:Hot Temperature; D008854:Microscopy, Electron; D009691:Nucleic Acid Denaturation; D014867:Water
D004269:DNA, Bacterial; D014867:Water; D003903:Deuterium