[Modification of Schmidt-Tannhauser's method of bacterial nucleic acid fractionation].
It was revealed that in fractionation of nucleic acids by Schmidt-Tannhauser's method up to 90% of the DNA of bacteria was destroyed in the course of alkaline hydrolysis changing into the RNA fraction (the alkali-labile fraction of the DNA). The authors suggest a modification of the method based on the precipitation of the alkali-labile DNA fraction from the RNA fraction with ethanol. This modified method permits to record over 96% of all the cell DNA of bacteria.
D001419:Bacteria / Q000032:analysis*; D005591:Chemical Fractionation / Q000379:methods*; D011232:Chemical Precipitation; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004926:Escherichia coli / Q000032:analysis; D000431:Ethanol; D011550:Pseudomonas aeruginosa / Q000032:analysis; D012329:RNA, Bacterial / Q000302:isolation & purification*; D014733:Vibrio / Q000032:analysis
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D000431:Ethanol
Modifikatsiia metoda Shmidta-Tannkhauzera dlia fraktsionirovanniia nukleinovykh kislot bakteriĭ