title: [Protein content of E. coli membrane under conditions of repressed and derepressed biosynthesis of alkaline phosphatase].
abstract: Protein content of membranes in wild type strains of E. coli K12 and K10 and in mutants defective in alkaline phosphatase regulator genes: E. coli C85 (R1-R2+p+) and E. coli C4(R1+R2-P+) under the conditions of repression and derepression of this enzyme was studied. Correlation between the content in membranes of minor component with the molecular weight 30,700 and the state of the regulatory system of alkaline phosphatase biosynthesis was shown. This protein was absent in the membranes of the repressed cells of wild type strains and in the membranes of nonrepressible (constitutive) mutant E. coli C4. Probably the protein with molecular weight 30,700 is a product of the regulatory gene R2 and its binding with the membrane determines its regulatory function.
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D004794:Enzyme Repression; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005809:Genes, Regulator; D008565:Membrane Proteins / Q000378:metabolism*; D009154:Mutation; D013045:Species Specificity
chemical_list: D008565:Membrane Proteins; D000469:Alkaline Phosphatase
vernacular_title: Izuchnie belkovogo sostava membran Escherichia coli s repressirovannym i derepressirovannym biosintezom shchelochnoĭ fosfatazy.