Antigenicites of fragments of Clostridium botulinum type B derivative toxin.
Two fragments with molecular weights of 110,000 and 60,000 were separated in a preparatory scale by gel filtration of the reduced Clostridium botulinum type B trypsinized derivative toxin on Sephadex G-200 with 0.05 M tris(hydroxymethyl)aminomethane-0.38 M glycine buffer, pH 8.3, containing 5 mM ethylenediaminetetraacetate, 1 mM dithiothreitol, and 2 M urea as eluant. They were both antigenic, forming crossing precipitin lines against type B antitoxin in agar gel double diffusion tests.
D000942:Antigens, Bacterial; D000992:Antitoxins; D001426:Bacterial Proteins / Q000032:analysis; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D003014:Clostridium botulinum / Q000276:immunology*; D003911:Dextrans; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D008970:Molecular Weight; D012967:Sodium Dodecyl Sulfate; D014118:Toxins, Biological; D014357:Trypsin; D014361:Trypsin Inhibitors
D000942:Antigens, Bacterial; D000992:Antitoxins; D001426:Bacterial Proteins; D003911:Dextrans; D014118:Toxins, Biological; D014361:Trypsin Inhibitors; D012967:Sodium Dodecyl Sulfate; D014357:Trypsin