Immunofluorescence and immunoperoxidase observations of anti-lactic dehydrogenase-1 antibody.
For immunoelectron-microscopic demonstration of lactic dehydrogenase (LDH), anti-subunit-B antibody was produced in rabbits by immunization with purified pig LDH-1 (B4) isoenzyme. Immunoglobin fragment Fab' was obtained from anti-LDH serum immunoglobulin G and conjugated with horseradish peroxidase. Subunit-B-containing LDH was present diffusely in the sarcoplasm of the pig heart muscle. There was concentration of LDH in the 1-band. LDH reaction was also present in the mitochondrial intermembranous space. The present immunoelectron-microscopic examination is in general agreement with the previous cytochemical demonstration of LDH using thiocarbamylnitro blue tetrazolium.
D000818:Animals; D000906:Antibodies; D005455:Fluorescent Antibody Technique; D006735:Horseradish Peroxidase; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D008854:Microscopy, Electron; D009206:Myocardium / Q000201:enzymology / Q000648:ultrastructure; D011817:Rabbits / Q000276:immunology; D013552:Swine
D000906:Antibodies; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D006735:Horseradish Peroxidase