Modification of the automated sequence determination as applied to the sequence determination of the Spirulina maxima ferredoxin.
The amino acid sequence of the Spirulina maxima ferredoxin was shown to be: H2N-Ala-Thr-Tyr-Lys-Val-Thr-Leu-Ile-Ser-Glu-Ala-Glu-Gly-Ile-Asn-Glu-Thr-Ile-Asp-Cys-Asp-Asp-Asp-Thr-Tyr-Ile-Leu-Asp-Ala-Ala-Glu-Glu-Ala-Gly-Leu-Asp-Leu-Pro-Tyr-Ser-Cys-Arg-Ala-Gly-Ala-Cys-Ser-Thr-Cys-Ala-Gly-Lys-Ile-Thr-Ser-Gly-Ser-Ile-Asp-Gln-Ser-Asp-Gln-Ser-Phe-Leu-Asp-Asp-asp-Gln-Ile-Gln-Ala-Gly-Tyr-Val-Leu-Thr-Cys-Val-Ala-Tyr-Pro-Thr-Ser-Asp-Cys-Thr-Ile-Gln-Thr-His-Gln-Glu-Glu-Gly-Leu-Tyr-COOH. The S. maxima ferredoxin is the first procaryote ferredoxin of the plant-algal type to be reported. A modification of the automated sequence determination of a peptide, which was extracted by the organic solvents used to remove excess reagents and the amino acid thiazoline, was utilized to complete the sequence of a 36 residue tryptic peptide.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000458:Cyanobacteria / Q000032:analysis*; D005288:Ferredoxins / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D014357:Trypsin
D000596:Amino Acids; D005288:Ferredoxins; D010446:Peptide Fragments; D014357:Trypsin