The primary structure of non-initiator methionine transfer ribonucleic acid from Bakers' yeast. II. Partial digestion with ribonuclease T1 and derivation of the complete sequence.
Large oligonucleotides obtained from partial RNase T1 digestion of tRNAMetm from bakers' yeast (S. cerevisiae, strain Y 185) were isolated by chromatographic procedures and sequenced. The complete sequence of the tRNAMetm was established from the results of this partial digestion, together with those of the complete RNase T1 and A [EC 3.1.4.8 and 22] digestions of tRNAMetm reported in the preceding paper. The structure of this tRNAMetm was arranged into a clover leaf comparable with those of other tRNAMet species including bakers' yeast initiator tRNA.
D001483:Base Sequence; D008715:Methionine / Q000032:analysis; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis* / Q000302:isolation & purification; D006163:Ribonuclease T1 / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D009843:Oligoribonucleotides; D012343:RNA, Transfer; D008715:Methionine; D006163:Ribonuclease T1