A new extractant for serum thyroxine by enzymatic digestion of thyroxine binding proteins.
The use of a pepsin solution to digest serum proteins results in quantitative release of thyroxine from carrier proteins. This extraction method greatly simplifies the original Murphy and Pattee (1) competitive protein-binding assay for serum thyroxine. The simplicity and efficiency of this extractant suggest that it may have application in radioimmunoassay and competitive protein-binding assay for nonpeptide ligands in serum.
D005069:Evaluation Studies as Topic; D006801:Humans; D007037:Hypothyroidism / Q000097:blood; D008832:Microchemistry; D010434:Pepsin A; D011869:Radioligand Assay / Q000379:methods; D013974:Thyroxine / Q000097:blood*; D013975:Thyroxine-Binding Proteins
D013975:Thyroxine-Binding Proteins; D010434:Pepsin A; D013974:Thyroxine