title: Transcription in vitro of phi29 DNA and EcoRI fragments by Bacillus subtilis RNA polymerase.
abstract: EcoRI fragments A, B and C produced from linear phi29 DNA, but not D or E fragments, are transcribed by purified Bacillus subtilis RNA polymerase. The transcription of fragments A and C is initiated preferentially with GTP and to a lesser extent with ATP; the reverse happens in the case of fragment B. The dinucleotides GpU and GpA respectively, compete specifically with the incorporation of [gamma-32P]GTP directed by fragments A and C. The RNA synthesized in vitro by purified B. subtilis RNA polymerase is highly asymmetric. Most of the RNA synthesis directed by fragments A and C is early RNA. However, most of the RNA produced by fragment B is anti-late-RNA. Addition of crude extracts inhibit the transcription of fragment B but not that of fragments A and C.
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D001435:Bacteriophages / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli; D007700:Kinetics; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases