title: [Regulation of asparaginase and glutaminase activity in Pseudomonas fluorescens mutants lacking in the ability to synthesize glutamic acid].
abstract: A mutant of Pseudomonas fluorescens, AG-M49, has been isolated, which lacks the ability to synthesize glutamic acid. The mutant contains both aspartase A and aspartase AG. The mutant was characterized by a considerably high activity of glutaminase and aspartase, increasing with the rate flow, during its continuous cultivation on a medium with L-glutamate. L-aspartate penetrates the cells and induces formation of the both deamidases in the mutant. In these conditions, the concentration of exocellular ammonia increases. Asparagine, but not glutamine, also stimulates production of the deamidases in the mutant.
mesh_terms: D001215:Asparaginase / Q000378:metabolism*; D003470:Culture Media; D005971:Glutamates; D005972:Glutaminase / Q000378:metabolism*; D009154:Mutation; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000254:growth & development
chemical_list: D003470:Culture Media; D005971:Glutamates; D001215:Asparaginase; D005972:Glutaminase
vernacular_title: Reguliatsiia asparaginaznoĭ i glutaminiaznoĭ aktivnosteĭ u mutanta Psuedomonas fluorescens, utrativshego sposobnost; sintezirovat' glutaminovuiu kislotu