title: Localization of acid phosphatase activity in the liver of pregnant rats.
abstract: In the liver of pregnant rats, fed ad libitum, there was an increase in acid phosphatase specific activity which occurred in two peaks, one at the 15th day and the other at the end of gestation. By light and electron microscopic histochemistry, the activity was found to be localized in parenchymal cell peribiliary dense bodies and also in phagosomes present in macrophages and parenchymal cells. There was an increase in liver wieght which reached a peak at the 17th day of gestation. Total DNA also rose to the 17th day; there was a high rate of cell division in the hepatic parenchyma at the 17th and 18th days of gestation. During this period single cell deletion by apoptosis was relatively frequent and in late pregnancy there was evidence of cell deletion by lysis. During pregnancy there was a slight increase in sinusoidal macrophages as a proportion of the total cell population but there did not appear to be significant changes in macrophage enzymic activity. It is suggested that the acid phosphatase activity present in macrophages makes a minor contribution to total liver activity, most of which is present in parenchymal cells. Acid phosphatase activity associated with single cell deletion appears to be quantitatively negligible. There was a direct relationship between total hepatic acid phosphatase activity and the numbers of peribiliary dense bodies, which were most numerous at the 15th day and at the end of gestation. It is suggested that these residual bodies contain products of detoxification processes and also cell structural elements resulting from enhanced liver metabolism and intracellular turnover during pregnancy.
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D004247:DNA / Q000032:analysis; D005260:Female; D005459:Fluorides / Q000494:pharmacology; D006056:Golgi Apparatus / Q000201:enzymology; D006651:Histocytochemistry; D008099:Liver / Q000032:analysis / Q000201:enzymology* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology; D008264:Macrophages / Q000201:enzymology; D009929:Organ Size; D011247:Pregnancy; D011270:Pregnancy, Animal; D051381:Rats; D013997:Time Factors
chemical_list: D004247:DNA; D000135:Acid Phosphatase; D005459:Fluorides