Mutant of Bacillus subtilis with a temperature-sensitive lesion in ribonucleic acid synthesis during germination.
We have isolated a mutant of Baccillus subtilis with a temperature-sensitive lesion in the process of spore germination. The temperature-sensitive mutation affects only germination and outgrowth, and the earliest defect observed is an early block of ribonucleic acid synthesis during germination at 46 C. Upon return to 35 C there is a complete repair of the impaired function, even in the absence of protein synthesis. Protein synthesis inhibition during germination of the mutant spores at 46 C has the effect of increasing the amount of ribonucleic acid made. The temperature-sensitive mutation is located near aroI.
D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002701:Chloramphenicol / Q000494:pharmacology; D002874:Chromosome Mapping; D005796:Genes; D009154:Mutation; D010641:Phenotype; D012329:RNA, Bacterial / Q000096:biosynthesis*; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism; D013696:Temperature; D014161:Transduction, Genetic
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D002701:Chloramphenicol