[Effect of calcium ions on sorption of phthalocyanin dye by membranes of individual nerve fibers].
The binding of vital anionic phthalocyanine dye Heliogen blue K (HBK) by crab axons has been studied cytophotometrically. HBK koes not penetrate the cell but stains membranes selectively. The removal of Ca-ions from the surrounding artificial sea water (a.s.w.) leads to an obvious desorption of an ion-bound fraction of HBK from the fibre, provided the removal of Ca-ions from a.s.w. is combinded with slight hypotonia or with addition of EDTA. The hypotonia, which has no effect on sorption, causes swelling of fibres facilitating the removal of Ca-ions from membrane, i.e. the effect similar to that of EDTA. In the Ca-ions devoid hypotonic solution, the decrease of sorbed dye makes up 1.4% per minute of the initial concentration, and in the hypotonic solution with EDTA - this decrease reaches 0.95%. It is suggested that the withdrawal of Ca-ions from the membrane results in conformational changes of the membrane protein component. These changes are expressed as redistribution of changes, detected with the aid of the HBK anion.
D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D003386:Brachyura; D002118:Calcium / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D006651:Histocytochemistry; D066298:In Vitro Techniques; D007211:Indoles / Q000378:metabolism*; D009994:Osmolar Concentration
D004396:Coloring Agents; D007211:Indoles; D004492:Edetic Acid; D002118:Calcium
Vliianie ionov kal'tsiia na sorbtsiiu ftalotsianinovogo krasitelia membranami odinochnykh nervnykh volokon