Bacteriophage MX-1: properties of the phage and its structural proteins.
Bacteriophage MX-1 is a virulent DNA phage for Myxococcus. The host range includes strains of Myxococcus xanthus, M. fulvus and M. virescens. The phage has a sedimentation coefficient (S degrees 20,w) of 1145S and a density of 1-531 g/ml. By using SDS-polyacrylamide gel electrophoresis, 23 phage proteins with apparent mol. wt. between 10000 and 150000 were resolved. Gel filtration in the presence of non-ionic detergent partially resolved the proteins. The fraction excluded from Sephadex G-100, fraction 1, contains two glycoproteins. Fraction 1 was resolved into three fractions (1-1, 1-2 and 1-3) by chromatography on Sephadex G-200. The glycoproteins were present in fraction 1-2; all the proteins from this fraction were derived from the phage tail. Comparison of the amino-acid, hexosamine and neutral-sugar compositions of the two glycoproteins showed that they are distinct molecular species; the smaller molecule is not a subunit of the larger. The significance of these findings is discussed and compared with the proteins of the tails of T-even phage of Escherichia coli.
D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D001435:Bacteriophages / Q000032:analysis / Q000254:growth & development / Q000648:ultrastructure; D002241:Carbohydrates / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D008242:Lysogeny; D008970:Molecular Weight; D009231:Myxococcales; D010455:Peptides / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis
D000596:Amino Acids; D000606:Amino Sugars; D002241:Carbohydrates; D004279:DNA, Viral; D006023:Glycoproteins; D010455:Peptides; D014764:Viral Proteins