Improved large-volume sampler for the collection of bacterial cells from aerosol.
A modified large-volume sampler was demonstrated to be an efficient device for the collection of mono-disperse aerosols of rhodamine B and poly-disperse aerosols of bacterial cells. Absolute efficiency for collection of rhodamine B varied from 100% with 5-mum particles to about 70% with 0.5-mum particles. The sampler concentrated the particles from 950 liters of air into a flow of between 1 and 2 ml of collecting fluid per min. Spores of Bacillus subtilis var. niger were collected at an efficiency of about 82% compared to the collection in the standard AGI-30 sampler. In the most desirable collecting fluids tested, aerosolized cells of Serratia marcescens, Escherichia coli, and Aerobacter aerogenes were collected at comparative efficiencies of approximately 90, 80, and 90%, respectively. The modified sampler has practical application in the study of aerosol transmission of respiratory pathogens.
D000336:Aerosols; D000391:Air Microbiology; D000818:Animals; D001412:Bacillus subtilis / Q000302:isolation & purification; D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000295:instrumentation*; D003470:Culture Media; D004754:Enterobacter / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005456:Fluorescent Dyes; D005780:Gelatin; D005990:Glycerol; D008892:Milk; D011136:Polysorbates; D012706:Serratia marcescens / Q000302:isolation & purification; D013171:Spores, Bacterial / Q000302:isolation & purification; D013696:Temperature; D014966:Xanthenes
D000336:Aerosols; D003470:Culture Media; D005456:Fluorescent Dyes; D011136:Polysorbates; D014966:Xanthenes; D005780:Gelatin; D005990:Glycerol