Purification of RNA from animal cells using diethyl-pyrocarbonate.
Extraction of RNA from animal cells by a method using diethyl-pyrocarbonate yielded 50-60% of the total RNA. RNA purified by a hot phenol-SDS method from adenovirus 2 infected cells showed about 9% homology with adenovirus DNA, and RNA purified by diethyl-pyrocarbonate-SDS showed over 7% hybridization. Profiles of RNA prepared by both methods were identical when studied by polyacrylamide gel electrophoresis.
D000256:Adenoviridae; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004047:Diethyl Pyrocarbonate; D000882:Haplorhini; D006801:Humans; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D010636:Phenols; D012313:RNA / Q000302:isolation & purification*; D051381:Rats; D012967:Sodium Dodecyl Sulfate
D010636:Phenols; D012967:Sodium Dodecyl Sulfate; D012313:RNA; D004047:Diethyl Pyrocarbonate