Microsomal cytochromes of Candida tropicalis grown on alkanes.
A comparison of methods used in isolating microsomes and in measuring microsomal cytochrome P-450 demonstrated that separation following protoplast lysis gave the best results. By this latter technique a high amount of cytochrome P-450 (0.2-0.3 nmol/mg) was recovered but cytochrome P-420, considered as the denatured form, was absent. The alkanes specifically induce cytochromes P-450 and b5 localized on the microsomes. The denaturation in vivo of cytochrome P-450 into cytochrome P-420 even occurs during storage at 1 degree C. This degradation is increased during preparation of subcellular fractions if no preventive measures are taken.
D000473:Alkanes / Q000378:metabolism* / Q000494:pharmacology; D002175:Candida / Q000201:enzymology*; D002736:Chloroplasts / Q000201:enzymology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism*; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D013053:Spectrophotometry
D000473:Alkanes; D003580:Cytochromes; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase