Analysis of rabbit brain polysomal poly (A+) mRNA by DNA excess hybridization.
DNA sequence representation in rabbit brain mRNA was examined by DNA excess hybridization. purified polysomal poly (A+) mRNA was labeled in vitro with [3H] dimethyl sulfate and reacted at DNA: RNA ratios of 5000 : 1 and 30 000 : 1. Poly(A+) mRNA hybridizied mainly to nonrepeated DNA with a smaller kinetic component which was complementary to repeated DNA sequences. The latter component was not due to nuclear or ribosomal RNA contamination. Poly(A-) RNA purified from brain polysomes hybridized to excess DNA as a single repeated component. Thermal denaturation profiles of the RNA - DNA hybrids indicated a high degree of fidelity in base pairing.
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D004247:DNA; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D011061:Poly A / Q000032:analysis*; D011132:Polyribosomes / Q000032:analysis*; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011817:Rabbits; D013696:Temperature
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A; D004247:DNA