title: Use of gum tragacanth overlay, applied at room temperature, in the plaque assay of fish and other animal viruses.
abstract: Fish cells derived from rainbow trout gonad or Atlantic salmon are sometimes damaged by the relatively high temperature of agar overlay widely used for plaquing animal viruses. This heat-induced cell damage can be avoided by the use of gum tragacanth, which may be applied at room temperature. When the medium was buffered with tris(hydroxymethyl)aminomethane-hydrochloride and NaHCO3, the plaque assay could be performed without the use of a CO2 incubator. Using this method, a number of animal viruses were plaqued on a variety of cell monolayers at different temperatures under atmospheric ocnditions.
mesh_terms: D000362:Agar; D000818:Animals; D002460:Cell Line; D005399:Fishes / Q000382:microbiology; D006066:Gonads; D012116:Resins, Plant; D012474:Salmon; D013696:Temperature; D014337:Trout; D010948:Viral Plaque Assay / Q000379:methods*; D014776:Virus Cultivation; D014780:Viruses / Q000302:isolation & purification*
chemical_list: D012116:Resins, Plant; D000362:Agar