Carbohydrate analysis of concanavalin A-reactive and concanavalin A-nonreactive mycobacterial polysaccharides.
Concanavalin A-nonreactive polysaccharide and 2 concanavalin A-reactive polysaccharides of differing concanavalin A affinities that had been purified from culture filtrates of Mycobacterium tuberculosis were subjected to carbohydrate analysis by gas and gas-liquid chromatography. Concanavalin A-nonreactive polysaccharide was found to be D-arabinogalactan, and the concanavalin A-reactive polysaccharide of highest affinity was found to be D-arabinomannan. The lower affinity concanavalin A-reactive polysaccharide was rich in arabinose and probably represented a mixture, with more than one polysaccharide present. Glucan was found to be a significant contaminant of all 3 polysaccharides.
D001089:Arabinose / Q000031:analogs & derivatives; D003208:Concanavalin A; D005690:Galactose / Q000031:analogs & derivatives; D008351:Mannans / Q000032:analysis*; D009169:Mycobacterium tuberculosis / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000032:analysis*
D008351:Mannans; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D003208:Concanavalin A; C012189:arabinomannan; D001089:Arabinose; D005690:Galactose