title: Heat sensitivity of porcine IgG.
abstract: The sensitivity to heat of porcine IgG was studied. The serum from immunized pigs was heated at 56 degrees C for 30 min as for decomplementation. The elution pattern of the serum proteins on an agarose gel column showed a dramatic change with the appearance of a large peak of the gel-excluded material. This peak contained mainly IgG molecules which still retained its antibody activity. This fact points to misinterpretations which can easily occur in 7S and 19S antibody recognition during the porcine immune response. Correlation is suggested of this property with the large number of interheavy chain disulfide bridges present in porcine IgG.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D002850:Chromatography, Gel; D003165:Complement System Proteins / Q000378:metabolism; D006386:Hemagglutination Tests; D006358:Hot Temperature; D007106:Immune Sera / Q000032:analysis; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis / Q000378:metabolism*; D007075:Immunoglobulin M / Q000032:analysis; D011817:Rabbits; D013552:Swine
chemical_list: D000906:Antibodies; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003165:Complement System Proteins