Design of a microculture chamber to observe cell division of bacterial L-forms in liquid medium.
A culture chamber is described that permits phase-contrast microscopic observations of the growth of stable L-form cells of Bacillus subtilis 168 in liquid medium.
D001412:Bacillus subtilis / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000295:instrumentation*; D002455:Cell Division; D007740:L Forms / Q000254:growth & development*; D008858:Microscopy, Phase-Contrast