The mode of action of immunological adjuvants. I. A new method to assess the adjuvant effect of substances as represented by mycobacterial adjuvants.
When mice were sensitized with sheep red blood cells (SRBC), the addition of adjuvant-active substances to the sensitizing antigen resulted in a highly significant increase in the mean diameter of plaques developed by spleen cells as measured by Jerne's plaque technique. The increase in plaque diameter was found to be a good measure for the adjuvant effect of such substances as mycobacterial adjuvants, because the phenomenon is specific, reproducible, sensitive and can be tested using a few number of mice.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D005260:Female; D006462:Hemolytic Plaque Technique; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology* / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial