Extraction and purification of lipoteichoic acids from Gram-positive bacteria.
Hot and cold, 80% aqueous phenol extraction procedures together with an aqueous extraction technique have been evaluated for the isolation of lipoteichoic acids from the cytoplasmic membrane of Gram-positive bacteria. Lipoteichoic acids of Staphlococcus aureus H, Micrococcus 2102, Baccillus subtilis 168, and Bacillus subtilis W-23 were examined as each of them emphasises a different problem of contamination. The purity of the lipoteichoic acids with respect to cell-wall material, nucleic acid, and protein is discussed together with the criteria of purity which enables critical structural analysis of lipoteichoic acids to be carried out.
D001412:Bacillus subtilis / Q000032:analysis; D001419:Bacteria / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D008837:Micrococcus / Q000032:analysis; D013045:Species Specificity; D013210:Staphylococcus / Q000032:analysis; D013682:Teichoic Acids / Q000032:analysis*
D008070:Lipopolysaccharides; D013682:Teichoic Acids