[Activities of protein synthesis during the development of lethal nucleocytoplasmic hybrids obtained by nuclear graft between Ambystoma mexicanum Shaw and Ambystoma dumerilii Duges (Amphibia, Urodeles)].
A comparative study of proteinograms and zymograms (LDH, MDH) has been carried out at different ontogenic stages, in Ambystoma mexicanum (A.m.), Ambystoma dumerilii (A.d.) and nucleocytoplasmic hybrids 2 n A.d/cytoplasm Am. It appears that 2 n A.d. nucleus, grafted in A.m. cytoplasm expresses only a part of its potentialities, which could account for lethality of the nucleocytoplasmic hybrids.
D000557:Ambystoma / Q000196:embryology* / Q000378:metabolism; D000818:Animals; D004592:Electrophoresis, Starch Gel; D004625:Embryo, Nonmammalian / Q000032:analysis; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D008291:Malate Dehydrogenase / Q000096:biosynthesis; D008297:Male; D053652:Nuclear Transfer Techniques; D010063:Ovum / Q000032:analysis; D014176:Protein Biosynthesis; D013045:Species Specificity; D014183:Transplantation, Heterologous
D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase
Activités de synthèses protéiques au cours du développement d'hybrides nucléocytoplasmiques léthaux obtenus par greffe nucléaire entre les espèces Ambystoma mexicanum Shaw et Ambystoma dumerilii Duges (Amphibiens, Urodèles)