Aminoacyl transfer RNA formation. V. Effect of ethylenediaminetetraacetate on isoleucyl transfer RNA formation stimulated by either spermine or Mg2+.
EDTA inhibited isoleucyl tRNA formation stimulated by spermine in Escherichia coli. It was found that the inhibition by EDTA was not due to contamination of the constituents of the reaction mixture with Mg2+ or to inhibition of the binding of spermine to tRNA by EDTA, but to the interaction of EDTA with isoleucyl-tRNA synthetase. These data suggest the isoleucyl-tRNA synthetase of E. coli contains some metal ion, which is necessary for the enzyme to catalyze the reaction.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D007533:Isoleucine-tRNA Ligase / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D013096:Spermine / Q000494:pharmacology*; D013997:Time Factors
D012329:RNA, Bacterial; D013096:Spermine; D012343:RNA, Transfer; D004492:Edetic Acid; D000604:Amino Acyl-tRNA Synthetases; D007533:Isoleucine-tRNA Ligase; D008274:Magnesium