Properties of bispecific rosette-forming cells. III.---Antigen binding cells with double specificity after immunization with two covalently linked antigens.
Adult C57B1/6 mice were immunized with bovine serumalbumin and horse radish peroxidase administered simultaneously but either separated or covalently linked by glutaraldehyde treatment. The percentage of double rosettes formed with both antigens (coupled to red cells) as compared to the total rosette forming cells reached 20% when mice had been injected by the conjugate whereas it remained less than 2% in case of separate antigen administrations. In addition, these bispecific rosettes were inhibited after anti-theta serum plus complement treatment.
D000818:Animals; D000918:Antibody Specificity; D000961:Antilymphocyte Serum; D004912:Erythrocytes; D005976:Glutaral; D006735:Horseradish Peroxidase / Q000276:immunology; D007104:Immune Adherence Reaction; D051379:Mice; D008810:Mice, Inbred C57BL; D012710:Serum Albumin, Bovine / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D012710:Serum Albumin, Bovine; D006735:Horseradish Peroxidase; D005976:Glutaral