title: Effects of aldosterone on Na+ transport in the toad bladder. II. The anaerobic response.
abstract: The action of aldosterone on active Na+ transport was assessed under aerobic and anaerobic conditions in the isolated urinary bladder of the toad, Bufo marinus. Aldosterone augmented the short-circuit current (Isc) under rigorous anaerobiosis. Four lines of evidence indicate that the increase in anaerobic Isc does not represent an equivalent increase in active Na+ transport: 1. Net Na transport, determined by isotopic fluxes, was the same in the aldosterone-treated and control quarter-bladders, and significantly greater than the simultaneously measured Isc-2. Amiloride, an inhibitor of the apical entry of Na+, did not reduce the steroid-dependent increase in the anaerobic Isc-3. Substitution of choline for Na+ in the mucosal medium reduced the magnitude of the anaerobic Isc values but did not eliminate the effect of aldosterone. 4. Addition of ouabain, a potent inhibitor of the Na+ pump, partially inhibited the effect of aldosterone on the anerobic Isc but a significant hormonal increment remained. The source of the anaerobic Isc was not identified; an effort was made, however, to determine the dependence of this current on glycolysis. During anaerobiosis, aldosterone increased the integral Isc by 42% but did not alter lactate production. These results suggest that the steroid-dependent increase in the anaerobic Isc may involve effects on permeability properties of the epithelium rather than on active transport systems.
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D000584:Amiloride / Q000494:pharmacology; D000693:Anaerobiosis; D000818:Animals; D001693:Biological Transport, Active; D002024:Bufo marinus; D003486:Cyanides / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*
chemical_list: D003486:Cyanides; D007773:Lactates; D000450:Aldosterone; D010042:Ouabain; D000584:Amiloride; D012964:Sodium