High-performance liquid chromatographic analysis of prostaglandins formed during in vitro incubations with prostaglandin 15-dehydrogenase.
Dinoprost, dinoprostone, and prostaglandin E1 were each separated from their major 15-keto metabolites by high-performance liquid chromatography on a microparticulate, bonded, reversed-phase column after conversion to their p-bromophenacyl esters. Detection and simultaneous quantitation of prostaglandins in 1.0 ml of a 5-muM solution is possible. The method was applied to monitor prostaglandins formed during in vitro incubations with prostaglandin 15-dehydrogenase from monkey lungs. The advantages of this technique for assessing enzyme purity and activity are discussed.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004951:Esterification; D000882:Haplorhini; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D066298:In Vitro Techniques; D008722:Methods; D011453:Prostaglandins / Q000032:analysis* / Q000378:metabolism; D011458:Prostaglandins E / Q000032:analysis / Q000378:metabolism; D011460:Prostaglandins F / Q000032:analysis / Q000378:metabolism; D013997:Time Factors
D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D000429:Alcohol Oxidoreductases; D006911:Hydroxyprostaglandin Dehydrogenases