[Pattern of antibody structure. The amino acid sequence of a monoclonal immunoglobulin L-chain of lambde-type, subgroup I (Bence-Jones-protein Vor.). A contribution to the elucidation of the origin of antibody specificity (author's transl)].
The experiments leading to the determination of the primary strucutre of Bence-Jones-Protein Vor. are reported. The variable part of this immunoglobulin polypeptide chain can easily be identified as a typical representative of subgroup I of the lambde-chains. The constant part is characterized by the Kern- and OZ+ markers. The sequence data of this protein are in complete agreement with the demands of the germ line theroy of antibody formation. To demonstrate the evolutionary origin of the V-henes, a phylohenetic tree of all completely sequenced Vk-, Vlambde-, and VH-chains is constructed. Teh evolutionary rate of immunoglobulins is calculated and compared to that of other protein families.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000918:Antibody Specificity; D001536:Bence Jones Protein / Q000032:analysis*; D002918:Chymotrypsin; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007146:Immunoglobulin lambda-Chains / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D014357:Trypsin
D000596:Amino Acids; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains; D010446:Peptide Fragments; D001536:Bence Jones Protein; D002918:Chymotrypsin; D014357:Trypsin
Zur Strukturregel der Antikörper. Die Aminosäuresequenz einer monoklonalen immunglobulin-L-Kette vom lambde-Typ, Subgruppe I (Bence-Jones-Protein Vor.) Ein Beitrag zur Aufklärung der Entsthung der Antikörperspezifitäten