Growth of measles virus in cultures of rat glioma cells.
The interaction of measles virus with RG-6 cells derived from rat glioma was investigated. When a culture of RG-6 cells was infected with measles virus, the synthesis of viral antigens was detected in very few cells, at most 5%. The apparent resistance to measles virus infection was also repeatedly found in all of the subclonal cells derived form RG-6 cells. Although all of the virus-synthesizing cells had the ability to form plaques on Vero cells, they produced only a reduced amount of infectious virus, i.e., 0.1 plaque-forming units per cell. These results imply the existence of some mechanism that regulates growth of measles virus in cultures of RG-6 cells. The transmission of genetic material of measles virus from infected RG-6 cells to Vero cells was not inhibited in the presence of antiviral serum. This fact may provide a basis for interpretation of the persistence of virus, in the presence of antibody, in patients with subacute sclerosing panencephalitis.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D002999:Clone Cells; D004492:Edetic Acid; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000276:immunology*; D007106:Immune Sera; D008457:Measles / Q000276:immunology; D008459:Measles virus / Q000254:growth & development*; D011817:Rabbits / Q000276:immunology; D051381:Rats; D012526:Satellite Viruses / Q000302:isolation & purification; D014357:Trypsin; D010948:Viral Plaque Assay; D014776:Virus Cultivation; D014779:Virus Replication
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera; D004492:Edetic Acid; D014357:Trypsin