[Differences in distribution of type I and type II collagens in the superficial and intermediary zones of articular cartilage].
Undecalcified frozen sections, 40 mu thick, were cut from the knee articular cartilage of young adult Baboons (Papio papio). The sections were freeze dried and the superficial and the intermediate zones were separated by microdissection under a binocular dissecting microscope. The material of each zone was cleaved with CNBr and the major peptides were analyzed by discelectrophoresis in SDS polyacrylamide. Peptides alpha, CB 3,5 and alpha, (II) CB 10 were used as characteristic markers for type I and type II collagen respectively. The method could detect as little as 5-10% of type I collagen of total collagen in mixtures. The thin superficial zone contains type I and type II collagen. The intermediate zone contains only type II collagen. The possible biological significance of this different anatomical distribution of the two types of collagen is discussed. This special distribution could explain the contradictory data from the literature concerning the presence of type I collagen in the articular cartilage.
D000818:Animals; D002358:Cartilage, Articular / Q000032:analysis*; D003094:Collagen / Q000032:analysis*; D003488:Cyanogen Bromide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D000882:Haplorhini; D010215:Papio; D010446:Peptide Fragments / Q000032:analysis
D010446:Peptide Fragments; D003094:Collagen; D003488:Cyanogen Bromide
Répartition différente du collagene de type I et du collagène de type II dans la zone superficielle et dans la zone intermédiaire du cartilage articulaire