[A low pressure perfusion method suitable for enzyme histochemical studies on the cochlea exemplified with the demonstration of NADH-diaphorase activity (author's transl)].
The use of a low pressure perfusion method for enzyme histochemical reactions in the cochlea of guinea pig is proved in the case of the demonstration of the NADH-diaphorase. It was shown by measuring of the microphonic potentials during the perfusion, that the perfusion medias caused practically no chemical or mechanical defections of the hair cells under the new conditions of working. The activities of the NADH-diaphorase were demonstrated mainly in the hair cells and in the nervous fibres of the organ of Corti. A remarkable NADH-diaphorase activity was detected in the Boettcher cells too.
D000818:Animals; D003051:Cochlea / Q000201:enzymology*; D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis*; D006168:Guinea Pigs; D006651:Histocytochemistry; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009925:Organ of Corti / Q000201:enzymology; D010477:Perfusion / Q000379:methods
D009252:NADPH Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase
Die Verwendbarkeit einer Niederdruckperfusionsmethode für enzymhistochemische Untersuchungen der Cochlea, dargestellt am Beispiel der NADH-Diaphorase