A simple method for the elimination of platelets from the lymphocyte-platelet mixture by sucrose.
A method for the separation of lymphocytes from human blood by Ficoll-Hypaque density centrifugation, with removal of platelets by a single sucrose solution with a density of 65%, is presented. The Ficoll-Hypaque-sucrose method gives a good yield of lymphocytes, high viability and purity, and relatively low contamination with erythrocytes and platelets.
D000925:Anticoagulants; D001792:Blood Platelets; D002499:Centrifugation, Density Gradient / Q000379:methods; D002951:Citrates; D004492:Edetic Acid; D006493:Heparin; D006650:Histocompatibility Testing; D006801:Humans; D008214:Lymphocytes; D013395:Sucrose
D000925:Anticoagulants; D002951:Citrates; D013395:Sucrose; D006493:Heparin; D004492:Edetic Acid