Separation of two new trypsin-inhibiting fractions from human serum.
Trace amounts of two unknown trypsin-inhibiting fractions have been separated from pooled human serum by DEAE-Sephadex chromatography. They are immunologically different from alpha1-antitrypsin, alpha2-macroglobulin or inter-alpha-trypsin inhibitor and they form two well separated peaks after rechromatography on DEAE-Sephadex column. Both of these fractions also inhibit chymotrypsin but not elastase. Their relation to other trypsin inhibitors of human serum is discussed.
D000818:Animals; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D006801:Humans; D005779:Immunodiffusion; D007700:Kinetics; D008259:Macroglobulins / Q000032:analysis; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D013552:Swine; D014361:Trypsin Inhibitors / Q000097:blood* / Q000302:isolation & purification; D000515:alpha 1-Antitrypsin / Q000097:blood
D008259:Macroglobulins; D014361:Trypsin Inhibitors; D000515:alpha 1-Antitrypsin; D002918:Chymotrypsin; D010196:Pancreatic Elastase