[RNA-fragments, necessary primers for the in vitro replication of DNA].
Partial degradation of ribosomal RNAs (E. coli rich in purine nucleotides) by different ribonucleases gives rise to the appearance of several families of RNA-fragments which after separation on "Sephadex G 25" were analyzed for base ratio, size and biological activity. In the presence of DNA dependent DNA polymerase, RNA-fragments act as primers for in vitro replication of DNAs from numerous sources.
D002250:Carbon Radioisotopes; D004261:DNA Replication; D004926:Escherichia coli; D066298:In Vitro Techniques; D012329:RNA, Bacterial / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000302:isolation & purification
D002250:Carbon Radioisotopes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal
ARN-fragments, amorceurs nécessaires à la réplication in vitro des ADN