In vitro modification and restriction of phage phi-105c DNA with Bacillus subtilis N cell-free extract.
The enzymes involved in host-controlled modification and restriction by Bacillus subtilis strain N were detected in cell free extracts. In the presenct of Mg2+ the N-specific endonucleases cleaved unmodified DNA but did not attack phi-105C. N DNA carrying N-specific modification. The restriction endonuclease required neither SAM nor ATP for its activity. The N-specific modification enzyme was active only in the presence of SAM, indicating that modification in this syteem is a methylation of DNA.
D000255:Adenosine Triphosphate; D001412:Bacillus subtilis; D002474:Cell-Free System; D003090:Coliphages; D004279:DNA, Viral; D004720:Endonucleases; D008274:Magnesium; D008745:Methylation; D012436:S-Adenosylmethionine
D004279:DNA, Viral; D012436:S-Adenosylmethionine; D000255:Adenosine Triphosphate; D004720:Endonucleases; D008274:Magnesium