The characterization of Euglena gracilis DNA by its reassociation kinetics.
The reassociation kinetics of Euglena gracilis DNA were carried out to determine the Cot 1/2 of the unique DNA (2000). The Cot 1/2 of the unique DNA and the amount of DNA per cell were used to show that this alga is not polyploid. Three kinetically definable classes of DNA were observed: a highly repetitive fraction, a middle repetitive fraction, and a non-repetitive fraction. Each fraction of DNA was characterized by its melting properties (Tm).
D000818:Animals; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D005056:Euglena gracilis / Q000032:analysis*; D006358:Hot Temperature; D007700:Kinetics; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation
D004247:DNA