Heterogeneity of the lipopolysaccharide from Pseudomonas aeruginosa.
Lipopolysaccharide isolated from pseudomonas aeruginosa PAC1 and its phage-resistant mutant was degraded by mild acid hydrolysis into lipid A and three major polysaccharide-containing fractions which were separated on Sephadex G-75. The low-molecular-weight fraction contained glucose, rhamnose, heptose, galactosamine, alanine and phosphate. The higher-molecular-weight fractions consisted mainly of glucose, rhamnose and glucosamine together with amino compounds. Alkaline degradation of the lipopolysaccharide produced at least four different species each of which contained a low-molecular-weight polysaccharide similar if not identical to that produced by acid hydrolysis. Under certain growth conditions an abnormal lipopolysaccharide was produced which was defective in the low-molecular-weight polysaccharide and contained mainly high-molecular-weight material. Strains of different serotype yielded lipopolysaccharides which also exhibited heterogeneity but contained a low-molecular-weight polysaccharide similar to that obtained from strain PAC1 and PAC1R. It is suggested that each strain of P. aeruginosa may produce several lipopolysaccharides each containing a polysaccharide common to all. The relative proportions of the various lipopolysaccharides may be changed by growth conditions.
D000409:Alanine / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006539:Heptoses / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D008970:Molecular Weight; D009154:Mutation; D010710:Phosphates / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000032:analysis* / Q000254:growth & development; D012210:Rhamnose / Q000032:analysis
D006539:Heptoses; D008070:Lipopolysaccharides; D010710:Phosphates; D005688:Galactosamine; D005947:Glucose; D005944:Glucosamine; D000409:Alanine; D012210:Rhamnose