Uptake and incorporation of pyrimidines in Euglena gracilis.
In photoorganotrophically grown cells of Euglena gracilis the uptake and incorporation degree of 12 different pyrimidines were tested. The rate of uptake of pyrimidines has distinct maxima in the late log phase and in the stationary phase of cell multiplication. The kinetics of uptake are linear in the first 2 h, do not show saturation at various concentrations and increase with the concetrations. No accumulation of the pyrimidines at various concentrations could be observed in the first 2 h of incubation. Membrane inhibitors as uranyl acetate inhibit the uptake of the reference substance alpha-AIB, which is wellknown transported by an active transport mechanism, but have no effect on uptake rate of uracil and cytosine. It could not be observed an energy requirement tested in temperature dependence and with electron transport inhibitors. Uptake of uridine, uracil, barbituric acid and alpha-AIB is inhibited by cycloheximide in a different manner after 5 - 10 min.
D001692:Biological Transport / Q000187:drug effects; D002455:Cell Division; D003513:Cycloheximide / Q000494:pharmacology; D005056:Euglena gracilis / Q000378:metabolism*; D011741:Pyrimidine Nucleosides / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism*; D013696:Temperature
D011741:Pyrimidine Nucleosides; D011743:Pyrimidines; D003513:Cycloheximide