Preparation and purification of lipid hydroperoxides from arachidonic and gamma-linolenic acids.
Commercial soybean lipoxygenase may be used under carefully controlled reaction conditions to give high yields of lipid hydroperoxides. Lipid hydroperoxides so derived from gamma-linolenic or arachidonic acid may be purified by high pressure liquid chromatography. Thus, commercial lipoxygenase serves as a viable source for 100 mg quantities of lipid hydroperoxides.
D001095:Arachidonic Acids / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D007536:Isomerism; D008042:Linolenic Acids / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis* / Q000302:isolation & purification; D008084:Lipoxygenase / Q000378:metabolism*; D010545:Peroxides / Q000096:biosynthesis*; D013696:Temperature; D013997:Time Factors
D001095:Arachidonic Acids; D008042:Linolenic Acids; D008055:Lipids; D010545:Peroxides; D008084:Lipoxygenase