Fertilization in vitro of Saimiri sciureus follicular oocytes.
An in vitro fertilization system was developed for the squirrel monkey. Oocytes recovered from follicles of gonadotropin-treated females and cultured in vitro with spermatozoa resulted in fertilized ova capable of developing to the four-cell stage in culture. In total, 264 follicular oocytes were recovered from 922 follicles in 38 Saimiri sciureus. Seventy-nine developed to the first polar body stage, and 32 of the 79 were fertilized in vitro.
D000818:Animals; D002455:Cell Division; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D003470:Culture Media; D004542:Ejaculation; D004558:Electric Stimulation; D005260:Female; D005306:Fertilization; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D000882:Haplorhini / Q000196:embryology*; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes / Q000032:analysis* / Q000502:physiology; D010060:Ovulation; D010063:Ovum / Q000032:analysis*; D012453:Saimiri / Q000196:embryology*; D013094:Spermatozoa / Q000502:physiology; D015053:Zygote / Q000032:analysis
D006063:Chorionic Gonadotropin; D003470:Culture Media; D005640:Follicle Stimulating Hormone