Genetic and physiological studies on the site of action of distamycin A.
Two new genetic loci of Bacillus subtilis are identified by mutations that confer resistance to distamycin A and to other antibacterial agents. The chromosomal region where they map probably contains a cluster of genes whose products are related to membrane structure and function. Some of the biological effects of distamycin A are still in evidence in the resistant mutants indicating that the drug possibly acts at multiple sites. Most biological effects of the drug (including the phenotypic correction of a morphopoietic mutation) are likely to be due to the interaction of distamycin A with membrane (or surface) structures.
D001412:Bacillus subtilis / Q000187:drug effects*; D001435:Bacteriophages; D001665:Binding Sites / Q000187:drug effects; D002874:Chromosome Mapping; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D004214:Distamycins / Q000378:metabolism / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005838:Genotype; D011758:Pyrroles / Q000494:pharmacology*; D011995:Recombination, Genetic / Q000187:drug effects; D012641:Selection, Genetic; D013997:Time Factors; D014161:Transduction, Genetic; D014170:Transformation, Genetic / Q000187:drug effects
D003470:Culture Media; D004269:DNA, Bacterial; D004214:Distamycins; D011758:Pyrroles