Synchronous cultures of micro-organisms: large-scale preparation by continuous-flow size selection.
The separation of the smallest cells from an exponentially growing culture by passage through a continuous flow centrifuge rotor under controlled conditions of rotor speed and flow rate, provides a simple method for the rapid production of large-scale synchronous cultures. The effluent from the rotor, containing the smallest-sized class of organisms, still suspended in their growth medium and still growing undisturbed throughout this rapid procedure, provides a culture which goes on to exhibit synchronous cell division. Successfully applied to budding and fission yeasts and to amoeboid and ciliated protozoa, this procedure ti potentially applicable to any non-filamentous, non-aggregating, unicellular organism or to cells of higher plants or animals growing in liquid tissue-cultures.
D000818:Animals; D002175:Candida / Q000254:growth & development; D002452:Cell Count; D002455:Cell Division; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation; D056890:Eukaryota / Q000254:growth & development*; D005069:Evaluation Studies as Topic; D012568:Schizosaccharomyces / Q000254:growth & development; D013769:Tetrahymena pyriformis / Q000254:growth & development; D013997:Time Factors; D015003:Yeasts / Q000254:growth & development*