Binding of the Fluorescent probes 1-anilinonaphthalene-8-sulfonate and N-phenyl-1-naphthylamine to bacteria.
Changes in fluorescence intensities in Pseudomonas fluorescens, Escherichia coli, Streptococcus thermophilus, and Bacillus subtilis were investigated by means of the fluorescence probes (1-anilinonaphthalene-8-sulfonate and N-phenyl-1-naphthylamine) during a temperature programmed run. A decrease in fluorescence intensity was observed in all investigated organisms when the temperature was elevated from 4 to about 40 degrees C. A strong increase in fluorescence intensity was found in gram-negative Ps. fluorescens and E. coli when the temperature was elevated over 40 degrees C. On the other hand, only a slight enhancement in fluorescence intensities was observed in gram-positive Str. thermophilus and B. subtilis when the lipid specific dye NPN was applied at elevated temperatures. Discontinuities in all the fluorescence temperature profiles were found between 20 and 30 degrees C in the case of both ANS and NPN. With ANS the fluorescence profiles turned upwards and with NPN downwards after the inflection points. The effects of KCl, NH4Cl, NaCl and LiCl on ANS binding differed from those detected for NPN. Saturation of probe binding was reached at lower salt concentrations in the case of NPN than ANS, but depended somewhat on temperature.
D015057:1-Naphthylamine / Q000031:analogs & derivatives / Q000378:metabolism*; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism*; D001412:Bacillus subtilis / Q000378:metabolism*; D002414:Cations, Monovalent / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism*; D005453:Fluorescence; D009281:Naphthalenes / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D013291:Streptococcus / Q000378:metabolism*; D013696:Temperature
D000817:Anilino Naphthalenesulfonates; D002414:Cations, Monovalent; D009281:Naphthalenes; D015057:1-Naphthylamine