[Intensive conversion in vitro of 5 alpha-androstane-3-alpha, 17 beta-diol into 5 alpha-dihydrotestosterone in the anterior hypophysis of male rats].
When anterior hypophysis from male rats were incubated in the presence of tritiated 5 alpha-androstan-3 alpha, 17 beta-diol (3 alpha-diol), the major part of recovered pituitary radioactivity was constituted by 5 alpha-dihydrotestosterone (5 alpha-DHT). This steroid represented an average of 72% of total tissular radioactivity. In cytosol, the bound hormonal fraction was constituted by about 47% 5 alpha-DHT and 35% 3 alpha-diol, whereas free radioactivity contained 67% 5 alpha-DHT and 22% 3 alpha-diol. These results demonstrate an intensive conversion of 3 alpha-diol into 5 alpha-DHT and consequently that the transformation of 5 alpha-DHT into 3 alpha-diol is reversible. Thus it is reasonable to think that the biological action of 3 alpha-diol one can observe following in vivo or in vitro experiments is due, in fact, to its conversion into 5 alpha-DHT.
D015113:Androstane-3,17-diol / Q000378:metabolism*; D000731:Androstanes / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000096:biosynthesis*; D007700:Kinetics; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D012995:Solubility
D000731:Androstanes; D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol
Intense transformation in vitro du 5 alpha-androstane-3 alpha, 17 beta-diol en 5 alpha-dihydrotestostérone dans l'hypophyse antérieure du Rat mâle