Novel interference in thiobarbituric acid assay for lipid peroxidation.
The thiobarbituric acid test for lipid peroxidation, when applied to a mixture of acetaldehyde and sucrose, produces a 532 nm aborbing chromogen which is indistinguishable from that formed by malonaldehyde and thiobarbituric acid. Unless special procedures are adopted to correct for this effect, the combined action of acetaldehyde and sucrose interferes seriously with the assay of lipid peroxidation reactions, notably those implicated in alcohol-induced liver injuries. However, this unusual thiobarbituric acid effect also can be used as a sensitive method for the detection of acetaldehyde.
D000079:Acetaldehyde / Q000032:analysis; D004492:Edetic Acid; D005069:Evaluation Studies as Topic; D007202:Indicators and Reagents; D008055:Lipids / Q000032:analysis*; D008722:Methods; D010545:Peroxides; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013858:Thiobarbiturates
D007202:Indicators and Reagents; D008055:Lipids; D010545:Peroxides; D013858:Thiobarbiturates; D004492:Edetic Acid; D000079:Acetaldehyde