title: A decalification method for ultrastructure of echinoderm tissues.
abstract: Decalcification of echinoderm tissues for electron microscopy can be achieved after glutaraldehyde-osmium fixation by treatment with a 1:1 mixture of 2% ascorbic acid and 0.3 M NaCl for 12-24 hours. Electron photomicrographs of material decalcified by this procedure are superior to those from EDTA-treated tissue and show few of the deleteriour effects produced by EDTA.
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D003648:Decalcification Technique; D004447:Echinodermata / Q000648:ultrastructure*; D004492:Edetic Acid / Q000494:pharmacology
chemical_list: D004492:Edetic Acid; D001205:Ascorbic Acid