[Rubella virus. II. Replication in Vero calls and effects of actinomycin Dand cycloheximide].
Budding at the plasmic membrane is the primary mode of production of rubella virus in Vero cells. Intense cytopathic effect is observed even if only 10% of the cells are infected. Actinomycin D has little effect on the multiplication of rubella virus but cycloheximide inhibits its growth. This inhibitiion is probably due to the reduction of cellular protein synthesis and not to a direct action of the inhibitorson the virus multiplication. Viral proteins in infected cells have not been demonstrated. The presence of a viral substance inhibiting normal cellular synthesis is discussed.
D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology*; D003588:Cytopathogenic Effect, Viral; D003609:Dactinomycin / Q000494:pharmacology*; D003864:Depression, Chemical; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D007668:Kidney; D012367:RNA, Viral / Q000096:biosynthesis; D012412:Rubella virus / Q000187:drug effects* / Q000254:growth & development; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication / Q000187:drug effects
D012367:RNA, Viral; D014764:Viral Proteins; D003609:Dactinomycin; D003513:Cycloheximide
Le virus de la rubéole. II. Réplication dans les cellules Vero et effects de I'actinomycine D et du cycloheximide.