Pseudomonas aeruginosa exotoxin in mice: histopathology and serum enzyme changes.
The histopathology and serum levels of mice inoculated intravenously with Pseudomonas aeruginosa exotoxin were studied. The toxin exerted a marked effect on the liver but elicited no demonstrable microscopic changes in other organs. The microscopic lesions caused in the liver by a single injection of two 50% lethal doses (LD50) of toxin (2.3 mug) were characterized by necrosis, cellular swelling, and fatty change within 4--8 hr and near total hepatocellular necrosis at 48 hr. Hepatic necrosis was accompanied by a parallel rise in serum levels of aspartate and alanine aminotransferases and alkaline phosphatase. A single injection of 10 LD50 elicited similar but somewhat more rapid degeneration. No progressive lesions were seen after injection of toxoid or of 0.5 LD50 of toxin.
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D005260:Female; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D051379:Mice; D011550:Pseudomonas aeruginosa; D014118:Toxins, Biological / Q000633:toxicity*; D014121:Toxoids / Q000633:toxicity; D000637:Transaminases / Q000097:blood*
D014118:Toxins, Biological; D014121:Toxoids; D000637:Transaminases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase