A Hg (II) induced conformational change in penicillinase.
Penicillinase (E.C. 3.5.2.6) from Bacillus cereus 569/H is inhibited by Hg(II). The inhibition is characterized by non-competitive kinetics and can be reversed by EDTA. A Hg(II) induced conformational change is indicated because: (1) The EDTA regenerated activity is unstable and is rapidly converted to an iodine-sensitive state, and (2) An irreversible change in the circular dichroism spectrum at 222 nm is found.
D001409:Bacillus cereus / Q000201:enzymology; D002413:Cations, Divalent; D002942:Circular Dichroism; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005453:Fluorescence; D007700:Kinetics; D008628:Mercury / Q000494:pharmacology*; D010405:Penicillinase / Q000302:isolation & purification; D011487:Protein Conformation; D013997:Time Factors; D065093:beta-Lactamase Inhibitors
D002413:Cations, Divalent; D065093:beta-Lactamase Inhibitors; D004492:Edetic Acid; D010405:Penicillinase; D008628:Mercury