The effect of glutaraldehyde on the stability of erythrocytes and on virus receptor substances.
Fixation with glutaraldehyde at a concentration above 0.05 per cent for 1 h at neutrality rendered erythrocytes resistant towards surfactants as Triton X-100, Tween-80 and sodium dodecyl sulphate. Fixation of erythrocytes with low concentrations of glutaraldehyde abolished the lytic effect of membrane active proteins as complement, phospholipase C and staphylococcal alpha-toxin. The fixation procedure did not alter significantly the receptor groups on erythocytes for viruses of the ortho- and para-myxo group and rubella virus. The fixation reduced the agglutinability of human O-erythrocytes by reovirus.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D002645:Chickens; D003165:Complement System Proteins; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D005976:Glutaral / Q000494:pharmacology*; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006801:Humans; D009975:Orthomyxoviridae / Q000276:immunology; D010740:Phospholipases / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D011817:Rabbits; D012087:Reoviridae / Q000276:immunology; D012412:Rubella virus / Q000276:immunology; D012756:Sheep; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014118:Toxins, Biological / Q000494:pharmacology; D014780:Viruses / Q000276:immunology*
D000447:Aldehydes; D011136:Polysorbates; D014118:Toxins, Biological; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D003165:Complement System Proteins; D010740:Phospholipases; D005976:Glutaral