Measurement of serum magnesium with a centrifugal analyzer.
We show how serum magnesium can be determined with a centrifugal analyzer. The method is based upon a manual procedure involving magnesium/calmagite complex formation in an alkaline reagent mixture. The assay, performed at 30 degrees C, requires 5 mul of serum in a final reaction volume of 405 mul, including 350 mul of reagent mixture. The stable color produced at 1 min is measured at 520 nm. Linearity studies showed that Beer's law was followed to 50 mg/liter. Average analytical recovery was 97.3%. Within-day studies showed CV's of 3.36% and 1.09%, compared to day-to-day variations of 5.32% and 3.04% at 18.8 and 46.6 mg of magnesium per liter, respectively. Correlation studies with the manual method, the Du Pont aca (methylthymol blue compleximetric) procedure, and atomic absorption spectroscopy showed correlation coefficients of 0.996, 0.976, and 0.950, respectively. Results compare excellently with those by common present methods. The method is fast, economical, reliable, and applicable to small specimen volumes.
D001391:Azo Compounds; D002498:Centrifugation; D007202:Indicators and Reagents; D008274:Magnesium / Q000097:blood*; D008722:Methods; D009282:Naphthalenesulfonates; D013053:Spectrophotometry; D014050:Toluene
D001391:Azo Compounds; D007202:Indicators and Reagents; D009282:Naphthalenesulfonates; D014050:Toluene; D008274:Magnesium