title: Regulated in vitro synthesis of the enzymes of the deo operon of Escerichia coli. properties of the DNA directed system.
abstract: The four enzymes deoxyriboaldolase, thymidine phosporylase, deoxyribomutase, and purine nucleoside phosphorylase have been synthesized in substantial amounts in a DNA-dependent in vitro system programmed with DNA containing the deo operon. The synthesis is greatly stimulated by deoxyribose-5-phosphate and cyclic AMP indicating that the deoR repressor and the catabolite activating protein (CAP) are highly active under our cell-free conditions. In contrast it has not yet been possible to observe a reproducible effect of the cytR repressor in vitro. The sequential appearance of active enzymes has confirmed the direction of transcription as being dra-tpp-drm-pup and has indicated that the four genes are transcribed into a single tetracistronic message.
mesh_terms: D000446:Aldehyde-Lyases / Q000032:analysis; D002474:Cell-Free System; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000032:analysis*; D003855:Deoxyribose; D004926:Escherichia coli / Q000201:enzymology*; D005809:Genes, Regulator; D005958:Glucosephosphates; D009876:Operon; D010428:Pentosephosphates; D010770:Phosphotransferases / Q000032:analysis; D011683:Purine-Nucleoside Phosphorylase / Q000032:analysis; D012267:Ribosemonophosphates; D013939:Thymidine Phosphorylase / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005958:Glucosephosphates; D010428:Pentosephosphates; D012267:Ribosemonophosphates; D003855:Deoxyribose; D011683:Purine-Nucleoside Phosphorylase; D013939:Thymidine Phosphorylase; D010770:Phosphotransferases; D000446:Aldehyde-Lyases; C020019:deoxyribose-phosphate aldolase