Tryptic cleavage of IgD at elevated temperature and isolation of a Fc-like fragment in high yield.
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006801:Humans; D006868:Hydrolysis; D007122:Immunoelectrophoresis; D007072:Immunoglobulin D; D007141:Immunoglobulin Fc Fragments / Q000302:isolation & purification*; D007138:Immunoglobulin delta-Chains / Q000032:analysis; D007146:Immunoglobulin lambda-Chains / Q000032:analysis; D008970:Molecular Weight; D014357:Trypsin; D014461:Ultracentrifugation
D007072:Immunoglobulin D; D007141:Immunoglobulin Fc Fragments; D007138:Immunoglobulin delta-Chains; D007146:Immunoglobulin lambda-Chains; D014357:Trypsin