title: Fatty acid biosynthesis during embryogenesis in the amphibian Bufo arenarum Hensel.
abstract: The fatty acid composition and biosynthesis of fatty acids were studied during early embryogenesis of the toad Bufo arenarum Hensel. The ova and stages up to the 6 1/2 day embryo have similar fatty acid compositions, with ca. 70% unsaturated acids. The eggs and embryo were permeable to acetate and impermeable to palmitic, linoleic, and eicosa-8, 11, 14-trienoic acid. Labeled acetate was incorporated by the eggs into the saturated acids-lauric, myristic, palmitic, stearic, arachidic, and behenic- and into the unsaturated acids-myristoleic, palmitoleic, oleic, and eicosaenoic acids. During segmentation and gastrulation, de novo biosynthesis of fatty acids increased, desaturation to myristoleic, palmitoleic, and oleic acids was enhanced; and fatty acids were esterified to triglycerides, phosphatidyl choline, and phosphatidyl ethanolamine. The feeding embryo (11 days) changed the pattern of incorporation to less incorporation into triglycerides.
mesh_terms: D015126:8,11,14-Eicosatrienoic Acid / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D000818:Animals; D002022:Bufo arenarum / Q000196:embryology* / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000201:enzymology / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D008041:Linoleic Acids / Q000378:metabolism; D010169:Palmitic Acids / Q000378:metabolism; D013997:Time Factors; D015053:Zygote / Q000378:metabolism
chemical_list: D000085:Acetates; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D010169:Palmitic Acids; D044943:Fatty Acid Desaturases; D015126:8,11,14-Eicosatrienoic Acid