Reconstitution of Rh (D) antigen activity from human erythrocyte membranes solubilized by deoxycholate.
Proteins were selectively solubilized from human erythrocyte membranes with deoxycholate. After ultracentrifugation and preliminary fractionation procedures, the detergent was removed from the soluble membranes by Bio-Beads SM-2 and dialysis against 5 millimolar magnesium chloride. Reaggregation took place with the apparent formation of vesicles which showed serologically specific Rh antigen activity.
D002458:Cell Fractionation; D002462:Cell Membrane / Q000276:immunology; D003840:Deoxycholic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D008970:Molecular Weight; D012204:Rh-Hr Blood-Group System / Q000302:isolation & purification*; D012995:Solubility; D014462:Ultrafiltration
D012204:Rh-Hr Blood-Group System; D003840:Deoxycholic Acid