D-Glucosamine as inhibitor of early processes of transformation in Bacillus subtilis 168 trp2.
Glucosamine added to a transformation medium (TM2) after a 30-min cultivation of cells exhibited the highest inhibitory effect on the transformation process in Bacillus subtilis 168 trp2. The recipient culture was least sensitive to glucosamine added after 50 min. Glucosamine had no inhibitory effect when added 10 min after the transformation DNA.
D001412:Bacillus subtilis / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D005944:Glucosamine / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D013237:Stereoisomerism; D013997:Time Factors; D014170:Transformation, Genetic / Q000187:drug effects*; D014364:Tryptophan / Q000096:biosynthesis
D004269:DNA, Bacterial; D014364:Tryptophan; D005947:Glucose; D005944:Glucosamine