[An effective immunodepressive treatment for demonstrating in allogeneic mice the tumorigenic properties of cells grown in vitro (author's transl)].
Short intensive treatment with N-diazoacetylglycine amide (DGA) before the inoculum, followed by prolonged daily administration of L-asparaginase (Asnase), was tested for its ability to elicit tumorigenic properties of fibroblast-like cells cultured in vitro. With this treatment progressive tumor growth was obtained in allogeneic mice injected with cells of a transformed subline. Results show that combined use of DGA and Asnase affords a higher probability of proving in vivo the tumorigenic properties of injected cells than in newborm or X-irradiated recipients. Experimental data indicate that L-asparagine depletion does not inhibit the in vitro growth of fibroblast-like cells.
D000818:Animals; D001215:Asparaginase / Q000494:pharmacology*; D001391:Azo Compounds / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D005347:Fibroblasts; D005998:Glycine / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D051379:Mice; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental
D001391:Azo Compounds; D007166:Immunosuppressive Agents; D001215:Asparaginase; D005998:Glycine
Un efficace trattamento immunodepressivo per la dimostrazione in topi allogenici della oncogenicità di cellule coltivate in vitro