The comparison of rat liver rough endoplasmic reticulum membrane proteins before and after in vitro removal of its bound ribosomes.
The comparison of the proteins of rat liver rough membrane after stripping with EDTA or KCl-puromycin by two dimensional gel electrophoresis is described. By stripping the membrane with EDTA, most of the basic ribosomal proteins are still attached to the membrane; in contrast to the EDTA stripping method, treatment with KCl-puromycin removes most of the ribosomal proteins and does not remove any of the membranal proteins.
D000818:Animals; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008566:Membranes / Q000032:analysis; D011132:Polyribosomes / Q000032:analysis; D011189:Potassium Chloride; D011691:Puromycin; D051381:Rats; D012269:Ribosomal Proteins / Q000032:analysis*; D012270:Ribosomes / Q000032:analysis*; D012995:Solubility
D012269:Ribosomal Proteins; D011691:Puromycin; D011189:Potassium Chloride; D004492:Edetic Acid