Attempts at differentiation of the structure of neurons in the corpus geniculatum laterale of the pig by the immunocytochemical technique.
The investigations were carried out on the corpus geniculatum laterale of the pig with the aim to differentiate the structure of its neurons on the basis of the antigenic properties of the nervous tissue. The antigenic material consisted of the homogenate of the corpus geniculatum laterale (H) and of its nuclear (P1) and mitochondrial (P2) fractions. The antigens were used to immunize 3 groups of rabbits, the 4th one serving as control. The antigens were prepared according to requirements of the technique. By immunization of the rabbits with the antigens immune sera were obtained. These sera were processed in accordance with the requirements of the technique. The authors conducted the studies employing the immunofluorescent technique after Coons with the use of the anti-rabbit IgG goat serum conjugated with fluorescein, as well as the immunohistochemical method according to Nakane with the use of the antibodies labelled with horseradish peroxidase. The investigations led the authors to conclude that H as well as P1 and P2 possess distinct antigenic traits. Moreover, the findings of the immunofluorescent and immunohistochemical tests in which the anti-H serum was used point to the localization of the antigen in the following nerve structures: neuron perikaryons, glia cells, and nerve fibres. On the other hand, the application of anti-P1 and anti-P2 sera permitted the antigens to be localized in the peripheral areas of the neuron nuclei and in the mitochondria. The results obtained with the immunofluorescent technique were in agreement with those produced immunohistochemically.
D000818:Animals; D005455:Fluorescent Antibody Technique; D005829:Geniculate Bodies / Q000166:cytology*; D006651:Histocytochemistry; D005779:Immunodiffusion; D009474:Neurons / Q000166:cytology*; D011817:Rabbits / Q000276:immunology; D013552:Swine