title: Physical characterization of Caulobacter crescentus flagella.
abstract: Preparations of intact flagella isolated from Caulobacter crescentus CB13B1a were found to contain two protein species of apparent molecular weights 28,000 and 25,000. Both proteins cross-reacted completely with each other and with purified flagella in Ouchterlony double-immunodiffusion assays. The amino acid compositions of the isolated proteins were similar to one another but precluded any precursor-product relationship. Absence of both the 25,000- and 28,000-molecular-weight proteins from a number of nonmotile mutants and the simultaneous reappearance of these proteins in a motile revertant provide further evidence of the relationship of these two proteins to flagellar structure.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001419:Bacteria / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D005407:Flagella / Q000032:analysis*; D005779:Immunodiffusion; D008970:Molecular Weight; D009068:Movement; D009154:Mutation
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins