A radioimmunoassay for the measurement of pyroglutamyl-histidyl-proline, a proposed thyrotropin releasing hormone metabolite.
A highly specific radioimmunoassay for the measurement of pGlu-His-Pro-OH (TRH-OH), a compound proposed to be a metabolite of pGlu-His-Pro-NH2 (TRH) has been developed. Using this assay experiments have been performed to study the inactivation of TRH and TRH-OH by serum. The results show that TRH-OH is inactivated in a fashion resembling the inactivation of TRH as has been described by others. Support for the deamidation mechanism as the main pathway for TRH degradation could not be achieved.
D000818:Animals; D001711:Biotransformation; D003429:Cross Reactions; D007457:Iodine Radioisotopes; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D013973:Thyrotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000032:analysis* / Q000378:metabolism
D007457:Iodine Radioisotopes; D013973:Thyrotropin-Releasing Hormone