Humoral factors affecting mouse peritoneal cell adherence reactions to Ascaris suum.
The abilities of normal mouse peritoneal exudate cells and fluids to mediate in vitro cell adherence reactions to Ascaris suum juveniles were investigated. Exudates were induced by intraperitoneal injection of Sephadex G-25 or by infection with A. suum and changes in the peritoneal cell populations were determined. The presence of a heat-labile component, probably complement, and a heat-stable component, probably immunoglobulin, in the peritoneal exudate fluid were required for optimum in vitro cell adherence reactions. The in vitro cell adherence reactions were inhibited by ethylenediaminetetraacetic acid; the inhibition was overcome by the addition of calcium and magnesium ions.
D000818:Animals; D000941:Antigens; D001200:Ascaris / Q000276:immunology*; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology; D002118:Calcium; D002452:Cell Count; D003165:Complement System Proteins; D003911:Dextrans; D004492:Edetic Acid; D005260:Female; D007104:Immune Adherence Reaction; D007136:Immunoglobulins; D008264:Macrophages / Q000276:immunology*; D008274:Magnesium; D051379:Mice; D008807:Mice, Inbred BALB C
D000941:Antigens; D003911:Dextrans; D007136:Immunoglobulins; D003165:Complement System Proteins; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium