Pyrimidine auxotrophy and the complementation map of the rudimentary locus of Drosophila melanogaster.
The complementation pattern of twelve rudimentary mutations have been analyzed at two different levels. When analyzed on the basis of complementation for a wing abnormality the mutations can be divided into three groups, each of which is believed to affect the activity of one of the first three enzymes of pyrimidine synthesis (Norby, 1973; Jarry and Falk, 1974; Rawls and Fristrom, 1975). However, when the mutants are analyzed for complementation on the basis of a second phenotype, pyrimidine auxotrophy, the distinction between two of these three groups is not evident. The disparity in the two patterns probably reflects a different threshold of gene activity required for the detection of an auxotrophic phenotype as compared to that at which a wing abnormality is detectable. The biochemical basis of these results is interpreted in light of recent data suggesting that at least the first two enzymes of pyrimidine synthesis are contained within a single multifunctional protein complex (Soderholm et al., 1975).
D000818:Animals; D003433:Crosses, Genetic; D004331:Drosophila melanogaster / Q000378:metabolism*; D005260:Female; D005816:Genetic Complementation Test; D007247:Infertility, Female; D008297:Male; D009154:Mutation; D010641:Phenotype; D011743:Pyrimidines / Q000378:metabolism*; D014921:Wings, Animal / Q000002:abnormalities*
D011743:Pyrimidines