Studies on a DNA photoreactivating enzyme from Streptomyces griseus. II. Purification of the enzyme.
A procedure is described for the isolation of a DNA photoreactivating enzyme from Streptomyces griseus. Application of chromatography on spherosil, ultraviolet-irradiated DNA-cellulose, DEAE-cellulose and single stranded-DNA-agarose resulted in a 22 000-fold purification with 46 percent recovery on the initial activity. According to polyacrylamide gel electrophoresis the final preparation was virtually homogeneous. The absorption spectrum of the enzyme exhibited a marked absorption band in the 400-460 nm region in addition to protein absorption.
D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D004260:DNA Repair; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D008190:Lyases / Q000302:isolation & purification*; D013053:Spectrophotometry; D013305:Streptomyces griseus / Q000201:enzymology*; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D008190:Lyases