The antigenicity in guinea pigs and monkeys of three mycobacterial polysaccharides purified by affinity chromatography with concanavalin A.
The antigenicity of 3 polysaccharides purified from culture filtrates of Mycobacterim tuberculosis by affinity chromatography using a concanavalin A-agarose absorbent was studied. All 3 purified polysaccharides were found to be potent elicitors of delayed skin test reactions in sensitized guinea pigs and in a tuberculos monkey. This antigenicity could not be attributed to contaminating protein. Small dermal reactions were also observed in control guinea pigs. All 3 polysaccharides reacted with precipitating antibody in guinea pig sera, the antigenic specificity observed with the guinea pig sera differing from that demonstrated with reference goat antiserum. The 3 polysaccharides were also demonstrated to contain hemagglutination antigenic sites.
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003208:Concanavalin A; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D000882:Haplorhini; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D005779:Immunodiffusion; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D012882:Skin Tests
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; D003208:Concanavalin A