Cell-free translation of immunoglobulin messenger RNA from MOPC-315 plasmacytoma and MOPC-315 NR, a variant synthesizing only light chain.
Total poly(A)-containing mRNA was isolated from the MOPC-315 and MOPC-315 NR plasmacytomas. The RNA was further fractionated on sodium dodecyl sulfate-sucrose gradients. The MOPC-315 mRNA fractions directed the synthesis of both the heavy chain and light chain precursor of the MOPC-315 IgA protein in a cell-free extract of Ehrlich ascites tumor cells. None of the MOPC-315 NR mRNA fractions tested programmed the synthesis of the heavy chain in this system. Analysis of cell-free products by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by immunoprecipitation demonstrated that no translatable heavy chain mRNA could be extracted from the MOPC-315 NR variant plasmacytoma.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D007070:Immunoglobulin A / Q000096:biosynthesis; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D008715:Methionine / Q000378:metabolism; D051379:Mice; D009194:Myeloma Proteins / Q000096:biosynthesis*; D009374:Neoplasms, Experimental / Q000378:metabolism; D010954:Plasmacytoma / Q000378:metabolism*; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D013462:Sulfur Radioisotopes
D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D007136:Immunoglobulins; D009194:Myeloma Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D013462:Sulfur Radioisotopes; D012967:Sodium Dodecyl Sulfate; D008715:Methionine