[Morphologic criteria of surface studies by electron microscopy and classification of lymphoproliferative syndromes. Critical study].
Surface associated immunoglobulins (s.Ig) have been detected on human lymphocytes, in normal individuals and in disease, by an immunoelectron microscopic method using peroxidase-labeled antibodies. Experiments have been carried out on fixed cell suspensions, in order to avoid membrane alterations induced by anti-immunoglobulin antibodies. Normal human blood B lymphocytes have a villous surface. However this relationship between microvilli and detectable s.Ig, as found in the normal state, is not confirmed by examinating various T and B cell proliferative states. Thus surface morphology alone is not sufficient for classifying cells in disease. The precise nature of mononuclear cells from hairy cell leukemia remains nuclear.
D001402:B-Lymphocytes / Q000276:immunology / Q000648:ultrastructure*; D006801:Humans; D007938:Leukemia / Q000648:ultrastructure*; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000648:ultrastructure; D008854:Microscopy, Electron; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology / Q000648:ultrastructure*; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology / Q000473:pathology*
D011947:Receptors, Antigen, B-Cell
Critères morphologiques de surfaces étudiés en microscopie électronique et classification des syndromes lymphoprolifératifs. Etude critique