[Transfer of adenovirus DNA into the nucleus at the beginning of infection of HeLa cells].
2 hrs after infection of HeLa cells with Ad 7, viral DNA was found in the nuclei. Inactivation of the virus with ultraviolet or antiserum, or pretreatment of the cells with chloroquine, did not affect the localisation of the DNA. The intranuclear DNA originated at least partly from viral particles passing through lysosomes. The appearance of Ad 7 DNA in the nucleus was delayed as compared to Ad 5 DNA.
D000256:Adenoviridae / Q000378:metabolism* / Q000528:radiation effects; D002467:Cell Nucleus / Q000378:metabolism / Q000382:microbiology*; D002738:Chloroquine / Q000494:pharmacology; D004279:DNA, Viral / Q000378:metabolism*; D006367:HeLa Cells; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism / Q000382:microbiology; D011830:Radiation Effects; D014466:Ultraviolet Rays
D004279:DNA, Viral; D002738:Chloroquine
Transfert de l'ADN d'adénovirus dans le noyau au début de l'infection des cellules HeLa