title: Studies on the nature of mammalian hypothalamic thyrotrophin releasing hormone using immunochemical, chromatographic and enzymic techniques.
abstract: Hypothalamic extracts from three mammalian species (rat, rabbit and sheep) were found to contain several ng of immunoreactive thyrotrophin releasing hormone (TRH)-like activity. This substance chromatographed on ion exchange chromatography (carboxymethyl cellulose) as a single peak that was indistinguishable from synthetic TRH. Hypothalamic TRH was also inactivated by normal human plasma at a rate (1.21-1.46%/mul plasma/h and 1.59-1.77%/50mul plasma/min) similar to that of synthetic TRH (1.42%/mul plasma/h and 1.73%/50mul plasma/min). This combination of chromatographic and enzymic techniques can be applied to the identification of immunoreactive TRH in body fluids.
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange; D004798:Enzymes / Q000097:blood; D005260:Female; D007031:Hypothalamus / Q000032:analysis / Q000378:metabolism*; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D012756:Sheep; D013973:Thyrotropin-Releasing Hormone / Q000097:blood / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D004798:Enzymes; D013973:Thyrotropin-Releasing Hormone