Gas-chromatographic analysis for succinimide anticonvulsants in serum: macro- and micro-scale methods.
A gas-chromatographic analysis for the succinimide anticonvulsant drugs--ethosuximide, methsuximide, and phensuximide--in 1.0 ml of serum was modified to improve its reliability, speed, and precision. A separate procedure for 10-100 mul of serum was also developed. Neither method requires an initial preparation of derivatives. The working range of each method is about 10-100 mg/liter for the macro-method, 2-100 mg/liter for the micro-method. The methsuximide metabolite, N-desmethylmethsuximide, is included in both methods. Concentrations of N-desmethylmethsuximide in the blood of two patients with petit mal epilepsy are reported.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D004832:Epilepsy, Absence / Q000097:blood / Q000188:drug therapy; D005013:Ethosuximide / Q000097:blood* / Q000627:therapeutic use; D008832:Microchemistry; D013388:Succinimides / Q000097:blood* / Q000627:therapeutic use; D013696:Temperature
D013388:Succinimides; D005013:Ethosuximide