Rickettsial cell water and membrane permeability determined by a micro space technique.
A micro space technique for determining membrane permeability in Rickettsia prowazeki is described and justified. The cell water, cell wall plus periplasmic volume, and glutamate, ethylene glycol, and adenosine diphosphate permeabilities were determined by this method. The effect of nonionic detergents on rickettsial permeability was examined: Triton X-100 destroyed the permeability barrier, whereas Lubrol-WX left it intact.
D000244:Adenosine Diphosphate / Q000378:metabolism; D001431:Bacteriological Techniques; D002463:Cell Membrane Permeability / Q000187:drug effects; D005026:Ethylene Glycols / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D007444:Inulin / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D012283:Rickettsia prowazekii / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology; D014867:Water / Q000378:metabolism
D005026:Ethylene Glycols; D005971:Glutamates; D013501:Surface-Active Agents; D014867:Water; D011092:Polyethylene Glycols; D013395:Sucrose; D000244:Adenosine Diphosphate; D007444:Inulin