Synthesis of immunoglobulin by substrate attached mouse myeloma cells.
Cultured mouse myeloma cells grow in suspension and synthesize and secrete large amounts of immunoglobulin. Mouse myeloma cells which attach to a plastic substratum have been obtained by mutagenesis and subsequent selection. Normal mouse myeloma cells will also attach to plastic tissue culture dishes pre-treated with poly-L-lysine. The attached cells synthesize and secrete the same large amounts of immunoglobulin as the suspended cells.
D000166:Acridines; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells / Q000378:metabolism; D007074:Immunoglobulin G / Q000096:biosynthesis; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D051379:Mice; D009101:Multiple Myeloma; D009154:Mutation; D011107:Polylysine / Q000494:pharmacology
D000166:Acridines; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins; D011107:Polylysine