Purification of human plasma kallikrein and urokinase by affinity chromatography.
L-Homoarginine benzylester was coupled with Sepharose 4B and used for affinity chromatography of human plasma kallikrein [EC 3.4.21.8] and urokinase [EC 3.4.99.26]. The euglobulin fraction of human plasma was treated with disodium ethylenediamine-tetraacetate (EDTA) and activated with acetone, and then kallikrein was purified 16-fold by affinity chromatography. The yield of kallikrein activity on affinity chromatography was 53%. The purified preparation of human plasma kallikrein appeared to be homogenous on gel electrophoresis. Commercial urokinase was also purified 4.8-fold with 64% yield by affinity chromatography.
D000096:Acetone; D002846:Chromatography, Affinity; D004492:Edetic Acid; D010450:Endopeptidases / Q000097:blood*; D006709:Homoarginine; D006801:Humans; D007610:Kallikreins / Q000097:blood* / Q000302:isolation & purification; D014568:Urokinase-Type Plasminogen Activator / Q000097:blood* / Q000302:isolation & purification
D000096:Acetone; D006709:Homoarginine; D004492:Edetic Acid; D010450:Endopeptidases; D007610:Kallikreins; D014568:Urokinase-Type Plasminogen Activator