Heavy chains of murine splenocyte membrane immunoglobulins: a comparison with the heavy chains of human and toad serum immunoglobulins.
The mobilities of murine splenocyte surface immunoglobulin heavy chains were compared by polyacrylamide gel electrophoresis in SDS-containing buffers, with those of the heavy chains of human EgM, IgG, and IgD, and toad IgY. Human zota-chain showed a mobility only slightly faster than that of human mu-chain, and required double-labeling techniques for unequivocal resolution. The mobility of the slower murine splenocyte surface heavy chain was identical to that of human mu-chain, but that of the second splenocyte heavy chain was significantly faster than that of human zota-chain, and similar to that of the heavy chain of toad IgY. Although the second murine splenocyte heavy chain is clearly distinct from mu-chain, no physicochemical evidence exists which relates it directly to human zota-chain.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002024:Bufo marinus; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007072:Immunoglobulin D / Q000032:analysis; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains / Q000032:analysis*; D007075:Immunoglobulin M; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000276:immunology
D007072:Immunoglobulin D; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell