Goat antibody directed against a human Leb blood group hapten, lacto-N-difucohexaose I.
A goat immunized with lacto-N-difucohexaose I (Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc) conjugated to polylysine (see article) produces antibody directed against the oligosaccharide. The antibody specifically precipitates Leb blood group substance and specifically binds lacto-N-difucohexaose I (Ka approximately equal to 4 X 10(6)M-1). Antibody purified by affinity chromatography is a 6.8S immunoglobulin of 158,000 +/- 6,000 molecular weight.
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification*; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D006041:Goats; D006241:Haptens; D006801:Humans; D007114:Immunization; D005779:Immunodiffusion; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides / Q000008:administration & dosage; D014461:Ultracentrifugation
D000906:Antibodies; D006241:Haptens; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides