Quantitation of Salmonella O-antibodies in human sera by enzyme-linked immunosorbent assay (ELISA).
The enzyme-linked immunosorbent assay (ELISA) has been applied to the detection of antibodies against Salmonella O-antigens in human sera. Phenol-water extracted lipopolysaccharides (LPS) from serogroups A (O-antigens 2, 12), B (4, 5, 12) and D (9, 12) were used as antigens. When compared to the tube agglutination method according to Widal employing sera from patients with verified or suspected typhoid--or paratyphoid fever and from healthy controls it was found that ELISA (i) correlated significantly with the Widal reaction, (ii) was up to 100-fold more sensitive, and (iii) showed a greater reproducibility.
D000042:Absorption; D000372:Agglutination Tests; D000469:Alkaline Phosphatase; D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial / Q000032:analysis*; D002850:Chromatography, Gel; D006801:Humans; D007106:Immune Sera; D008070:Lipopolysaccharides; D010284:Paratyphoid Fever / Q000276:immunology; D011135:Polysaccharides, Bacterial; D012475:Salmonella / Q000276:immunology*; D012485:Salmonella typhi / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology; D014435:Typhoid Fever / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D007106:Immune Sera; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D000469:Alkaline Phosphatase