Identification of specific crosslinked histones after treatment of chromatin with formaldehyde.
Treatment of chromatin or nuclei with formaldehyde or glutaraldehyde results in the appearance of crosslinked histones as analyzed on SDS gels. The identity of two major crosslinked products was determined by iodinating the product and analzying the tryptic peptides. The results show that one product is a dimer of histones IIb2 and IIb1 and another product is a dimer of histones IIb2 and IV. These species represent no more than 15% of the total cellular histone.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects; D002642:Chick Embryo; D002700:Chloramines; D002843:Chromatin / Q000032:analysis / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D004900:Erythroblasts / Q000032:analysis; D004912:Erythrocytes / Q000032:analysis; D005557:Formaldehyde / Q000494:pharmacology*; D005976:Glutaral / Q000494:pharmacology; D006657:Histones / Q000032:analysis*; D007455:Iodine; D010455:Peptides / Q000032:analysis; D014357:Trypsin / Q000378:metabolism; D014443:Tyrosine / Q000032:analysis
D002700:Chloramines; D002843:Chromatin; D006657:Histones; D010455:Peptides; D005557:Formaldehyde; D014443:Tyrosine; D007455:Iodine; D014357:Trypsin; D005976:Glutaral