Effect of carbon dioxide on the formation of alpha-amylase by Bacillus subtilis growing in continuous and batch cultures.
The influence of CO2 on the information of alpha-amylase by Bacillus subtilis NCIB 8646 growing in continuous and batch cultures was investigated. Different levels of CO2 examined in the batch cultures stimulated the formation of alpha-amylase, with the highest activity being obtained using 6% CO2 (v/v). The additions of CO2 inhibit the growth and division of vegetative cells of B. subtilis when CO2 is present in a concentration of more than 3% (v/v). In chemostat cultures, air containing 8% CO2 (v/v) increased the specific enzyme productivity almost three times over the control, without affecting the cell growth. An attempt is made to correlate the obtained results with the alesis.
D000681:Amylases / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology*; D002245:Carbon Dioxide / Q000494:pharmacology*; D002455:Cell Division; D003470:Culture Media; D007700:Kinetics; D000516:alpha-Amylases / Q000096:biosynthesis*
D003470:Culture Media; D002245:Carbon Dioxide; D000681:Amylases; D000516:alpha-Amylases