[Production of spheroplasts from Pseudomonas aeruginosa].
The spheroplasts were prepared from the gram-negative bacterium Pseudomonas aeruginosa IBFM-13 upon incubation with lysozyme, but without EDTA. The following conditions were found to be optimal: 20 percent sucrose; lysozyme, 54 mcg/mg protein of the cells; pH 7.6. The spheroplasts, as well as the cells, have a high rate of malate oxidation, but do not oxidize NADH2, the latter fact suggesting that the cytoplasmic membrane remains intact.
D004305:Dose-Response Relationship, Drug; D009113:Muramidase / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000187:drug effects; D013104:Spheroplasts / Q000302:isolation & purification*
D009113:Muramidase
Poluchenie sferoplastov iz Pseudomonas aeruginosa