title: Repair deficiency, mutator activity, and thermal prophage inducibility in dna-8132 strains of Bacillus subtilis.
abstract: A ts mutation, dna-8132 (Hara and Yoshikawa, 1973), in the region of chromosome replication origin of Bacillus subtilis was found to cause pleiotropic effects at a permissive temperature (30 C). Strains carrying this mutation were lethan at 48 C but exhibited higher spontaneous mutation frequency and a lower capacity for repairing radiation damages at 30C. Introduction of the polA59 (Gass et al., 1971) mutation further enhanced the repair deficiency and the mutator activity. These results suggest that the dna-8132 gene product may be directly involved in chromosome replication and repair. SPO2 lysogens carrying this mutation produced mature phages upon a temperature shift from 30 to 48 C. Phage production at nonpermissive temperature suggests that there are few defects in the precursors of deoxyribonucleic acid synthesis in the mutant.
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D001435:Bacteriophages / Q000254:growth & development*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004352:Drug Resistance, Microbial; D005720:Gamma Rays; D005796:Genes; D008242:Lysogeny; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D011830:Radiation Effects; D013696:Temperature; D014466:Ultraviolet Rays; D014779:Virus Replication
chemical_list: D004269:DNA, Bacterial; D008937:Mitomycins; D008769:Methylnitronitrosoguanidine; D015112:4-Nitroquinoline-1-oxide; D008741:Methyl Methanesulfonate