title: Characterization of monoclonal gammopathies, a new approach.
abstract: A new approach to the characterization (typing) of L- and H-chains of monoclonal immunoglobulins and Bence-Jones protein utilizing elution of a monoclonal immunoglobulin in conjunction with solid-support electrophoresis and the reverse Mancini procedure of radial immunodiffusion on Mylar-backed cellulose acetate membranes is described. A number of benefits are derived, particularly the use of only a fraction (1/200) of the antisera used with agar immunoelectrophoresis. The importance of "standardizing" commercial antisera (and a method for carrying this out) with the techniques described is emphasized.
mesh_terms: D001536:Bence Jones Protein / Q000032:analysis*; D001797:Blood Protein Electrophoresis; D006801:Humans; D006942:Hypergammaglobulinemia / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D007106:Immune Sera / Q000592:standards; D005779:Immunodiffusion / Q000379:methods; D007122:Immunoelectrophoresis; D007128:Immunoglobulin Fragments / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis; D007143:Immunoglobulin Heavy Chains / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis
chemical_list: D007106:Immune Sera; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D001536:Bence Jones Protein