A simple carbohydrate fermentation test for identification of the pathogenic Neisseria.
The carbohydrate fermentation test in cystine-Trypticase agar-tubed medium was compared with the Minitek system with carbohydrate-impregnated paper disks in Müeller-Hinton broth for identification of Neisseria gonorrhoeae and N. meningitidis. There was 100% agreement between the methods for confirmation of N. meningitidis. The paper disk method confirmed 98% of the N. gonorrhoeae isolates; the cystine-Trypticase agar method confirmed 96%. Reactions with the paper disk method could be read in 4 h.
D001431:Bacteriological Techniques; D050260:Carbohydrate Metabolism; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005285:Fermentation; D009343:Neisseria / Q000145:classification* / Q000378:metabolism; D009344:Neisseria gonorrhoeae / Q000145:classification / Q000378:metabolism; D009345:Neisseria meningitidis / Q000145:classification / Q000378:metabolism
D003470:Culture Media