[Isolation of 6 subunits of scorpion, Androctonus australis garzonii, hemocyanin].
A method for the purification of the 5 sub-units 5 S and of a 7 S sub-unit of the haemocyanin of the Scorpion Androctonus australis garzonii is reported. It includes preparation of crude haemocyanin, dissociation in 1 M urea in the absence of divalent cations, starch gel electrophoresis and A 50 DEAE "Sephadex" separation. All the sub-units are homogeneous with regard to ultracentrifugation and polyacrylamide gel electrophoresis. The yield variers from 7 to 20 per cent according to each fraction.
D000818:Animals; D006433:Hemocyanins / Q000032:analysis*; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000302:isolation & purification; D012605:Scorpions
D046911:Macromolecular Substances; D010446:Peptide Fragments; D006433:Hemocyanins
Méthode d'isolement de 6 sous-unités de l'hémocyanine du scorpion Androctonus australis garzonii