Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli.
The thymidylate synthetase gene of B. subtilis bacteriophage Phi-3-T, when cloned in plasmids pSC101 or pMB9 is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi-3-T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi-3-T, but not those of plasmid vectors.
D001412:Bacillus subtilis / Q000378:metabolism*; D001435:Bacteriophages / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000378:metabolism; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005111:Extrachromosomal Inheritance; D005796:Genes; D005809:Genes, Regulator; D005818:Genetic Engineering; D008780:Methyltransferases / Q000378:metabolism*; D010957:Plasmids; D013940:Thymidylate Synthase / Q000378:metabolism*; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral; D008780:Methyltransferases; D013940:Thymidylate Synthase; D004262:DNA Restriction Enzymes