Mating-reactive membrane vesicles from cilia of Paramecium caudatum.
Membrane vesicles with a high mating reactivity were obtained from cilia of Paramecium caudatum by treatment with a solution containing 2 M urea and 0.1 mM Na2-EDTA. All processes of conjugation were induced in cells of the complementary mating type by approximately 10 mug/ml proteins of the vesicles. Electron microscope observation showed that the membrane vesicles have a diameter of 100-150 nm. Electrophoretic analysis on SDS polyacrylamide gel revealed no significant difference in polypeptide patterns of the particles from the two complementary mating types.
D000818:Animals; D002458:Cell Fractionation; D002923:Cilia / Q000648:ultrastructure*; D003227:Conjugation, Genetic; D004492:Edetic Acid; D008566:Membranes / Q000648:ultrastructure; D009940:Organoids / Q000032:analysis; D010247:Paramecium / Q000502:physiology / Q000648:ultrastructure*; D011506:Proteins / Q000032:analysis; D014508:Urea
D011506:Proteins; D014508:Urea; D004492:Edetic Acid