A two-stage method for cross-linking antibody globulin to ferritin by glutaraldehyde. III. Size and antibody activity of the conjugates.
Immunoferritin conjugates consist of conjugates of different size. They can be separated in 4.5% polyacrylamide gel electrophoresis into at least 4 bands. The amount of the smallest 1:1 conjugate, in which one antibody molecule is linked to one ferritin molecule, is highest in coupling products with low overall yields of conjugated ferritin. Therefore relatively mild reaction conditions are recommended. With increasing size the antibody binding capacity of the conjugate is reduced.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D001666:Binding Sites, Antibody; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000302:isolation & purification; D005976:Glutaral; D007136:Immunoglobulins / Q000302:isolation & purification; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology
D000906:Antibodies; D007136:Immunoglobulins; D012709:Serum Albumin; D005293:Ferritins; D005976:Glutaral