[Comparison of nutrient media for isolation of microflora in swine dysentery].
The usability of five nutrient media - three kinds of spirolate media, thioglycolate medium and brain hearth medium - suitable for the isolation of Vibrio coli and germs similar to borrelia isolated from pigs affected by dysentery, and vibria isolated from cattle, was compared in the study. The results of comparison show that fresh-prepared substrates must be used bor the isolation of the mentioned germs in all cases and a corresponding medium must be provided. The modified spirolate media prepared from inland and imported ingredients were suitable for the isolation of the mentioned microorganisms even in the case of their use as selective media after enrichment with antibiotics. The spirolate media and thioglycolator agar were not found suitable for maintaining the strains of germs similar to borrelia due to the quick production of involution forms and the result dying of the strains. Thenutrient solution prepared as the brain hearth inf. Oxoid blood agar is very suitable for the isolation as well as maintenance of strains.
D000818:Animals; D003470:Culture Media; D004405:Dysentery, Bacillary / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014733:Vibrio / Q000302:isolation & purification*
D003470:Culture Media
Porovnanie zivných médií k izolácii mikroflóry pri dyzentérii osípaných