[Enzyme induction in Streptomyces hydrogenans, VI. Studies on the induction of 20beta-hydroxysteroid dehydrogenase, using immunological methods].
Antiserum against crystallized 20beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was used for various immunodiffusion and immunoprecipitation tests to show an increase of the de novo synthesis of 20beta-hydroxysteroid dehydrogenase by Streptomyces hydrogenans after cultivation of the cells in the presence of 11beta,21-dihydroxy-4,17(20)-pregnadien-3-one. Half lives of mRNA for 20beta-hydroxysteroid dehydrogenase in induced cells and of total mRNA in non-induced cells were calculated to be 126 s and 66 s, respectively. In vivo, 20beta-hydroxysteroid dehydrogenase appears to consist of four equal subunits. The monomers, molecular weight of 27 300, show a high tendency to form dimers and tetramers in the absence of dissociating agents. The aggregation is completely reversible in the presence of increasing concentrations of sodium dodecylsulfate.
D000818:Animals; D004586:Electrophoresis; D004790:Enzyme Induction / Q000187:drug effects; D006207:Half-Life; D006913:Hydroxysteroid Dehydrogenases / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D005779:Immunodiffusion; D008970:Molecular Weight; D011233:Precipitin Tests; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits / Q000276:immunology; D012967:Sodium Dodecyl Sulfate; D013302:Streptomyces / Q000201:enzymology*
D012329:RNA, Bacterial; D012333:RNA, Messenger; D012967:Sodium Dodecyl Sulfate; D006913:Hydroxysteroid Dehydrogenases