title: Sites of action of D2O in intact and skinned crayfish muscle fibers.
abstract: The effect on tension development of replacing 90% of the H2O of the bathing saline with D2O was studied on intact single fibers, and on skinned fibers before and after the latter were treated so as to eliminate Ca-accumulation by the sarcoplasmic reticulum (SR). Excitation-contraction coupling (ECC) of intact fibers is not abolished, but is depressed by D2O so that higher depolarizations are required to elicit a given tension. The reduction in tension at a given level of depolarization is not due to inhibition of the contractile system. The latter showed an enhanced Ca sensitivity; that is, skinned fibers respond to Ca concentrations that are 1-2 orders of magnitude smaller in D2O than in H2O saline. When bathed in D2O saline, intact fibers or skinned fibers with functional SR can still accumulate and release Ca in sufficient quantities to allow repeated induction of maximum tensions. Relaxation is slowed in all three types of preparation, perhaps because of an increased affinity of troponin to Ca in D2O salines.
mesh_terms: D000818:Animals; D003400:Astacoidea; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D003903:Deuterium / Q000494:pharmacology*; D004533:Egtazic Acid / Q000494:pharmacology; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008564:Membrane Potentials; D009119:Muscle Contraction / Q000187:drug effects*; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D012519:Sarcoplasmic Reticulum / Q000378:metabolism / Q000502:physiology; D014336:Troponin / Q000378:metabolism
chemical_list: D014336:Troponin; D002110:Caffeine; D008345:Manganese; D004533:Egtazic Acid; D011189:Potassium Chloride; D003903:Deuterium; D011188:Potassium; D002118:Calcium; D005998:Glycine