Genetic analysis of bacteriophage phi 29 of Bacillus subtilis: integration and mapping of reference mutants of two collections.
Reference mutants of Bacillus subtilis phage phi 29 of the Madrid and Minneapolis collections were employed to construct a genetic map. Suppressor-sensitive and temperature-sensitive mutants were assigned to 17 cistrons by quantitative complementation. Three-factor crosses were used to assign an unambiguous order for the 17 cistrons. Recombination frequencies determined by two-factor crosses were used to construct a linear genetic map of 24.4 recombination units. The genes were numbered sequentially from left to right (1 to 17) according to their relative map position.
D001412:Bacillus subtilis; D001435:Bacteriophages; D002874:Chromosome Mapping; D005796:Genes; D005816:Genetic Complementation Test; D009154:Mutation; D011995:Recombination, Genetic; D013489:Suppression, Genetic; D013696:Temperature