title: Relationship between the recirculation of lymphocytes and protein concentration of lymph in sheep.
abstract: The concentration and output of lymphocytes in lymph from the popliteal node in sheep were varied by antigenic stimulation of the lymph node and by intravenous administration of bovine anti-lymphocyte serum (ALS). The 4-5-fold increase in output and concentration of lymphocytes in lymph following antigenic stimulation of the node was accompanied by a 25-35% increase in the concentration of total protein and albumin. The above changes were not observed in afferent lymph draining the site of antigen injection. There was a 75-80% decrease in concentration of lymphocytes in lymph from the unstimulated popliteal lymph node following the administration of ALS and this was accompanied by decreases of 10-35% in the concentration of albumin and immunoglobulin. Essentially the same changes were observed when ALS was administered 6 days after antigenic stimulation of the node. Administration of ALS to sheep with cannulated intestinal lymphatic ducts was followed by a 75-80% reduction in lymphocyte concentration and in decreases ranging from 12-50% in the concentration of albumin, IgM and IgG-2. It is suggested that a significant loss of plasma protein is associated with the migration of lymphocytes between the endothelial cells of the post-capillary venules in lymph nodes.
mesh_terms: D000418:Albumins / Q000032:analysis; D000818:Animals; D000942:Antigens, Bacterial; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002003:Brucella abortus / Q000276:immunology; D002417:Cattle; D002465:Cell Movement; D006614:Hindlimb; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007422:Intestines; D008196:Lymph / Q000032:analysis*; D008198:Lymph Nodes; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D011506:Proteins / Q000032:analysis*; D012756:Sheep
chemical_list: D000418:Albumins; D000942:Antigens, Bacterial; D000961:Antilymphocyte Serum; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011506:Proteins