Studies on the immune response to fixed antigens. Preferential induction of helper function with heavily trinitrophenylated sheep erythrocytes, and glutaraldehyde-treated sheep erythrocytes.
Mice primed with heavily trinitrophenylated sheep red cells (TNP128SRC) or glutaraldehyde-treated sheep red cells (G-SRC) developed an early helper function mediated by thymus-derived cells. Such mice were able to produce high secondary responses to both hapten and carrier after challenge 2 days after priming, with lightly trinitrophenylated SRC (TNP0.14SRC). However, the primary response of the TNP128SRC or G-SRC-primed mice were very low to undetectable, and their secondary responses were also low when the challenge antigen was administered 4 days after priming or later. Inhibitory humoral factor(s) which were induced in the primed animals appeared responsible for the decreased capacity of primed mice to mount a secondary response when challenged later than 2 days after priming. Transfer of spleen cells from TNP128SRC-primed mice to sublethally irradiated recipients circumvents their exposure to inhibitory humoral factor(s) present in intact animals allowing them to react with challenge antigen. Enriched populations of T cells, but not B cells, were able to transfer this early immunologic memory to irradiated recipients. The theoretical and practical implications of these results are discussed.
D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D004912:Erythrocytes / Q000276:immunology*; D005976:Glutaral / Q000378:metabolism*; D007111:Immunity, Cellular; D007116:Immunization, Passive; D007156:Immunologic Memory; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D012756:Sheep; D013154:Spleen / Q000276:immunology
D000447:Aldehydes; D000941:Antigens; D009578:Nitrobenzenes; D005976:Glutaral