[Combination of the " hemagglutination inhibition reaction and the antiglobulin test for the purposes of the serological diagnosis of influenza (author's transl)].
The assessment of the HI test combined with the antiglobulin test in which antisera to human IgG, IgM and IgA were used as reagents as a method increasing the rate of detection of specific seroconversions to influenza viruses in patients and vaccinees is presented. The modified HI test increased the per cent of serological confirmation of influenza in patients from 49.3% in the routine HI test to 86.3%, and in subjects immunized with live A/Hong Kong/68 vaccine local serological changes were detected in 40% against 15% by the routine HI test. In the blood sera from adult patients the enhancing effect was obtained with IgG antiserum, in children with IgM antiserum. In nasopharyngeal washings the HI test gave better results when antiserum to IgA was employed. The utilization of the hi test combined with antiglobulin test considerably increases the volume and content of information obtained in influenza serodiagnosis by the routine HI test.
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D000936:Antigen-Antibody Complex; D002648:Child; D003298:Coombs Test; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007114:Immunization; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007252:Influenza Vaccines / Q000008:administration & dosage; D007251:Influenza, Human / Q000175:diagnosis* / Q000276:immunology; D009305:Nasopharynx / Q000276:immunology; D012698:Serologic Tests / Q000379:methods; D013997:Time Factors
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007252:Influenza Vaccines
Sovmeshchenie reaktsii tormozheniia gemaggliutinatsii i antiglobulinovoĭ proby v tseliakh serologicheskoĭ diagnostiki grippa