title: Mucoid strains of Pseudomonas aeruginosa: the influence of culture medium on the stability of mucus production.
abstract: Mucoid strains of Pseudomonas aeruginosa isolated from the respiratory tract of patients with cystic fibrosis (CF), those obtained from non-CF patients, and those obtained in vitro by the action of phage, were found to be stable in their mucoid colonial form when serially subcultured on deoxycholate-citrate agar. The ability of anionic, cationic and neutral surfactants to stabilise mucus production is described. The possible importance of dipalmitoyl lecithin as a stabilising agent for mucus production in vivo is considered, with particular reference to the role of mucoid P. aeruginosa in CF disease.
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D002593:Cetrimonium Compounds / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology; D003470:Culture Media; D003550:Cystic Fibrosis / Q000382:microbiology; D003840:Deoxycholic Acid / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology; D006801:Humans; D007785:Lactose / Q000494:pharmacology; D009093:Mucus / Q000378:metabolism*; D009499:Neutral Red / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013045:Species Specificity; D013501:Surface-Active Agents / Q000494:pharmacology; D013885:Thiosulfates / Q000494:pharmacology
chemical_list: D002593:Cetrimonium Compounds; D002951:Citrates; D003470:Culture Media; D005290:Ferric Compounds; D010713:Phosphatidylcholines; D011136:Polysorbates; D013501:Surface-Active Agents; D013885:Thiosulfates; D003840:Deoxycholic Acid; D009499:Neutral Red; D012967:Sodium Dodecyl Sulfate; D007785:Lactose; D000111:Acetylcysteine