Polygalacturonase of Botrytis cinerea E-200 Pers.
A polygalacturonase (poly(1,4-alpha-D-galacturonide)glycanohydrolase, EC 3.2.1.15) was purified from the culture fluid of Botrytis cinerea. The polygalacturonase preparation, homogeneous on the basis of disc-gel electrophoresis also showed pectinesterase activity. Some properties of the purified polygalacturonase were studied. It had a molecular weight about 69 000. It was inactivated by p-chloromercuribenzoate, tetranitromethane and urea. A 50% loss in viscosity of sodium polypectate solution occurred when 4.6% of the glycosidic bonds were hydrolyzed. The only end product of sodium polypectate and oligogalacturonides hydrolysis was monogalacturonic acid.?
D002729:Chloromercuribenzoates / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004492:Edetic Acid / Q000494:pharmacology; D004589:Electrophoresis, Disc; D005690:Galactose; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D006603:Hexuronic Acids; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007531:Isoflurophate / Q000494:pharmacology; D007700:Kinetics; D003904:Mitosporic Fungi / Q000201:enzymology*; D011134:Polysaccharides / Q000378:metabolism; D013329:Structure-Activity Relationship; D013774:Tetranitromethane / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D002729:Chloromercuribenzoates; D006603:Hexuronic Acids; D011134:Polysaccharides; D007531:Isoflurophate; D014508:Urea; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D006026:Glycoside Hydrolases; D013774:Tetranitromethane; D005690:Galactose