title: Nonenzymatic conversion of human hexosaminidase A.
abstract: Incubation of purified hexosaminidase A with merthiolate, parahydroxymercuribenzoic acid, or silver ions resulted in the formation of an enzyme which was identical, in all respects tested, with hexosaminidase B. Its electrophoretic mobility was identical to hexasaminidase B at three different pH levels. Its chromatographic properties, thermostability, and immunologic reactivity with specific anti-hexosaminidase A and anti-hexosaminidase B antisera were indistinguishable from hexosaminidase B. Conversion could be prevented by GSH but was not reversed by GSH once it had occurred. Conversion of hexosaminidase A to hexosaminindase B was accompanied by the appearance of an electrophoretically rapid, catalytically inactive protein. Hexosaminidase B, itself, was unaltered by incubation with merthiolate. These findings support a previously proposed model of hexosaminidase structure in which hexosaminidase A is (alphabeta)3 and hexosamindase B is (betabeta)2. On treatment of hexosaminidase A with merthiolate and other converting agents alpha and beta subunits are presumably dissociated and they reassociate as (betabeta)3, that is, hexosaminidase B. The expected free or polymerized catalytically inactive, alpha chains are detected on acrylamide gel electrophoresis. We suggest that the catalytic site of human hexosaminidase may be present only on the beta subunit and that the alpha subunit influences the substrate specificity of the enzyme, particularly as directed toward GM2.
mesh_terms: D002845:Chromatography; D004592:Electrophoresis, Starch Gel; D006596:Hexosaminidases / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D066298:In Vitro Techniques; D008626:Mercuribenzoates; D008956:Models, Chemical; D012836:Silver Proteins; D013329:Structure-Activity Relationship; D013696:Temperature; D013849:Thimerosal
chemical_list: D007106:Immune Sera; D008626:Mercuribenzoates; D012836:Silver Proteins; D013849:Thimerosal; D006596:Hexosaminidases