title: Detection reagent for adenine, guanine, uracil, cytosine and their alkylated bases, nucleotides and nucleosides on thin-layer plates.
abstract: The detection of alkylated bases at the nanogram level is reported. The reaction is based on two steps, first, chlorination of the bases and, secondly, spraying the chlorinated product with o-tolidine solution to give a dark blue color. The detection of nucleotides and nucleosides is also possible. The optimum conditions and the possible mechanism of the reaction are discussed.
mesh_terms: D000225:Adenine / Q000032:analysis*; D000478:Alkylation; D002713:Chlorine; D002855:Chromatography, Thin Layer; D003596:Cytosine / Q000032:analysis*; D006147:Guanine / Q000032:analysis*; D007202:Indicators and Reagents; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D014498:Uracil / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D009705:Nucleosides; D009711:Nucleotides; D002713:Chlorine; D014498:Uracil; D006147:Guanine; D003596:Cytosine; D000225:Adenine