In vivo cell synchrony in the L1210 mouse leukaemia studied with 5-fluorouracil or 5-fluorouracil followed by cold thymidine infusion.
[3H]-TdR and [3]-udR labelling indices and mitotic indices were followed in tumour-bearing mice after application of either 5-fluorouracil (FU) alone or of FU followed by cold TdR infusion. With FU alone, accumulation of cells at the beginning of S was found, but there was no indication of a synchronous passage of the accumulated cells further round the cycle. When FU injection was followed by cold TdR infusion, a synchronous passage of the accumulated cells through the cycle was observed. However, there was a large variation in the response of individual mice to this treatment.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005472:Fluorouracil / Q000378:metabolism / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000378:metabolism*; D051379:Mice; D008940:Mitotic Index / Q000187:drug effects; D009368:Neoplasm Transplantation; D013936:Thymidine / Q000378:metabolism*
D004247:DNA; D005472:Fluorouracil; D013936:Thymidine