Studies on the structure and possible function of the RNA "cap" in developing sea urchins.
Paracentrotus lividus embryos at the hatching blastula stage very quickly incorporate radioactivity from labeled nucleosides (except uridine) or 32P- or methyl-labeled methionine into a portion of the RNA that has been identified as a "cap". The most probable sequence of this cap is m7G (5') ppp (5')mAmpCp. A very active "capping" and methylation of the "cap" of preexisting RNA molecules was shown to occur at the blastula stage.
D000818:Animals; D001483:Base Sequence; D003609:Dactinomycin / Q000494:pharmacology; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D008745:Methylation; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012617:Sea Urchins
D012333:RNA, Messenger; D003609:Dactinomycin