title: Antibody-mediated suppression of tumor growth. I. Suppression by murine IgG1 isolated from alloantiserum.
abstract: The tumor suppressive activity of murine IgG1 antibody was studied in vivo. IgG1 was isolated from hyperimmune alloantisera against a murine lymphoma, 6C3HED, by absorption with heat-killed, formalinized Staphylococcus aureus. Cowan strain I, followed by DEAE ion exchange chromatography and Sephadex G-200 gel filtration chromatography. The isolated IgG1, which had no detectable IgM, IgA, IgG2a, IgG2b or IgG3, could suppress the growth of the tumor. In addition, DEAE and Sephadex G-200 column profiles of the in vivo tumor suppressive activity showed good correlation with the profiles of total IgG1 immunoglobulin and anti-tumor antibody assayed for IgG1 heavy chains and kappa light chains. The IgG1 tumor suppressive activity was not diminished after heating at 56 degrees C for 30 min.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002471:Cell Transformation, Neoplastic; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006358:Hot Temperature; D007106:Immune Sera; D007074:Immunoglobulin G / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D007166:Immunosuppressive Agents; D007518:Isoantibodies / Q000032:analysis; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011863:Radioimmunoassay
chemical_list: D007106:Immune Sera; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D007518:Isoantibodies