[Preparative approach to mannobiose and laminaribiose (author's transl)].
For synthetic purposes mannobiose is isolated from the polysaccharide mannan of ivory nut flower. The chain length of mannan A is determined to approximately 15 mannose units on average with a definite distribution from 5 to 25 mannose units. Acetolysis is optimized and yields 41% mannobiose. The separation on Sephadex G-15 yields pure mannobiose, and in addition its higher homologues in gram amounts. Detection of the eluates is performed using a differential refractometer. Correspondingly laminaribiose can be obtained from the polysaccharides laminaran (brown algae Laminaria hyperborea) and pachyman (fungus Poria cocos Wolf, Bukuryo) by selectively optimized acetolysis in 30% yield. Depending on the starting material separations are favourably performed on silica gel or on Sephadex G-15. Laminaribiose and its higher homologues are obtained in gram amounts.
D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D004187:Disaccharides / Q000032:analysis / Q000302:isolation & purification*; D005658:Fungi / Q000032:analysis; D008351:Mannans / Q000032:analysis / Q000302:isolation & purification; D008970:Molecular Weight; D000459:Phaeophyceae / Q000032:analysis; D010944:Plants / Q000032:analysis; D011134:Polysaccharides / Q000302:isolation & purification
D004187:Disaccharides; D008351:Mannans; D011134:Polysaccharides; C014637:laminaribiose; C014647:mannobiose
Präparative Zugänge zu Mannobiose und Laminaribiose