An electron stain for elastic fibers using orcein.
Orcein was found to be useful as an electron-opaque stain for elastic fibers in epoxy-sections. Ultra-thin sections of aorta were treated with elastica stain containing 0.1-0.3% orcein and counterstained in uranyl acetate and lead citrate. Elastic fibers were densely and specifically demonstrated in the stroma and near smooth muscle cells. The result of orcein staining has a comparable appearance under both light and electron microscopes.
D000368:Aged; D000818:Animals; D001013:Aorta, Thoracic / Q000648:ultrastructure*; D004549:Elastin / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D011817:Rabbits
D004549:Elastin