[The occurrence of neuraminidase and N-acylneuraminate pyruvate lyase in Haemophilus paragallinarum and Haemophilus paravium n.sp (author's transl)].
14 strains of H. paragallinarum, isolated from fowls suffering from infectious coryza or received from other investigators for comparison, and a collective consisting of 9 strains of the new species H. paravium nova species, also isolated from fowls, were shown to possess both enzymes, neuraminidase (EC 3.2.1.18) and N-Acylneuraminate-pyruvate lyase (EC 4.1.3.3). The detection of the enzymes was made initially by paper chromatography using N-acetyl-neuraminyllactose as substrate and secondly by the colorimetric assay of Warren's thiobarbituric acid method using N-acetylneuraminyllactose, colomic acid, Na salt, bovine submaxillary mucin, human serum and furthermore N-acetyl-neuraminate as substrates for neuraminidase and N-acylneuraminate pyruvate lyase respectively. The enzyme activities of the strains of H. paravium are stronger than those of H. paragallinarum.
D000818:Animals; D002645:Chickens / Q000382:microbiology*; D002854:Chromatography, Paper; D003124:Colorimetry; D006190:Haemophilus / Q000145:classification / Q000201:enzymology*; D006192:Haemophilus Infections / Q000382:microbiology; D009439:Neuraminidase / Q000032:analysis*; D007652:Oxo-Acid-Lyases / Q000032:analysis*; D011201:Poultry Diseases / Q000382:microbiology; D012141:Respiratory Tract Infections / Q000382:microbiology; D012794:Sialic Acids
D012794:Sialic Acids; D009439:Neuraminidase; D007652:Oxo-Acid-Lyases
Neuraminidase und N-Acylneuraminat-Pyruvat-Lyase bei Haemophilus paragallinarum und Haemophilus paravium n.sp