In vivo incorporation of isotopically labelled amino acids into vertebrate proteins: L-[Me-3H]methionine incorporation into chicken egg white lysozyme.
The in vivo incorporation of L-[Me-3H]methionine into egg white lysozyme of the laying hen has been examined. Using a versatile synthetic chicken diet which consisted of 75% free amino-acid ration and 25% normal laying ration, 5-8% incorporation of the [3H]methionine into lysozyme was demonstrated. The utility of this vertebrate in vivo incorporation technique is discussed in terms of its application to the incorporation of 13C-enriched amino acids into vertebrate proteins as a prelude to macromolecular 13C nuclear magnetic resonance studies.
D000818:Animals; D002645:Chickens; D004529:Egg White; D005260:Female; D008715:Methionine / Q000378:metabolism*; D009113:Muramidase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D010058:Oviposition
D008715:Methionine; D009113:Muramidase