In vitro function of granulocytes isolated from blood of normal volunteers using continuous-flow centrifugation in the IBM-aminco celltrifuge and adhesion-filtration leukapheresis using nylon fiber.
Granulocytes were harvested from each of five healthy male volunteers once by continuous flow centrifugation with the IBM-Aminco Celltrifuge, and once by adhesion filtration leukapheresis with nylon fiber. Granulocyte recovery and purity were significantly better with the filtration leukapheresis system than with continuous flow centrifugation. Measurements of trypan blue dye exclusion and muramidase activity were similar to those in control granulocytes regardless of the method of isolation. Granulocyte-stimulated oxygen consumption was diminished in granulocytes prepared by the adhesion filtration method, but normal in those prepared by continuous flow centrifugation with the IBM-Aminco Celltrifuge.
D001803:Blood Transfusion; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002498:Centrifugation / Q000379:methods; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D017708:Leukocyte Transfusion; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D009113:Muramidase / Q000097:blood; D010101:Oxygen Consumption
D009113:Muramidase