Estimation of glucose turnover with stable tracer glucose-1-13C.
Glucose turnover was measured in normal and disbetic dogs by the dilution of glucose-1-13C and glucose-1-14C tracers infused simultaneously at constant rates. In order to quantify the stable isotope, an enzymatic assay for the analysis of glucose-1-13C was developed and evaluated. CO2 from C-1 glucose was evolved by coupling hexokinase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconic dehydrogenase activities. The 13C/12C ratio of the CO2 was measured with a high-precision magnetic-deflection double-collector mass spectrometer, and the radioactivity of 14CO2 was quantified by liquid scintillation. The ratio of 13C/12C was reproducible in assays of CO2 evolved from either naturally occurring or 13C-enriched glucose. Furthermore, systemic glucose production rates measured with 13C- and 14C-labeled tracers were similar over a wide range from 2 to 12 mg./kg.-min. Thus, glucose-1-13C may be employed as a tracer for glucose metabolism in human subjects without incurring the risk of radiation.
D000818:Animals; D002245:Carbon Dioxide; D002247:Carbon Isotopes; D005591:Chemical Fractionation; D004285:Dogs; D005947:Glucose / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods*
D002247:Carbon Isotopes; D002245:Carbon Dioxide; D005947:Glucose