[Investigation of a new method for the determination of glucose, using the Greiner Selective Analyzer II (GSA II) (author's transl)].
The hexokinase method and a method using glucose dehydrogenase are compared. In general, the two methods show good agreement. The samples were not deproteinized and were obtained from fasting patients of the medical, surgical, rheumatological and hemodialysis clinics. The possible influence of anticoagulants, preservatives and hemoglobin on the determination of glucose with glucose dehydrogenase was checked; there was no evidence of interference. Among the carbohydrates which were tested, only 2-deoxyglucose gave a positive reaction. In addition, this is a reliable and cheap method. The multiplication factor for the photometric analysis remains stable for seven days with a linearity between 0.55 and 44.4 mmol/1 glucose.
D000429:Alcohol Oxidoreductases; D000925:Anticoagulants; D001322:Autoanalysis / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D001793:Blood Preservation; D005189:False Positive Reactions; D006593:Hexokinase; D006801:Humans; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000925:Anticoagulants; D001786:Blood Glucose; D000429:Alcohol Oxidoreductases; D006593:Hexokinase
Erfahrungen mit einer neuen Glucosebestimmungsmethode auf dem Greiner Selective Analyzer II (GSA II)