[Studies on the activation of complement by encapsulated and non-encapsulated staphylococci after their extraction with guanidinium chloride (author's transl)].
All demonstrable enzymes and toxins of encapsulated staphylococci (KS) were removed by extraction with guanidinium chloride. The capsules, however, remained apparently intact on the extracted (KS-Gu) staphylococci (fig. 1), as well as clumping factor and protein A. KS and KS-Gu failed to activate complement in the absence of specific antibodies. They showed neither immunadherence (table 1) nor agglutination by an antiserum against C3 (table 2). KS and KS-Gu had no significant chemotactic effects in vitro upon bovine granulocytes (fig. 2).
D000371:Agglutination; D000818:Animals; D002417:Cattle; D002633:Chemotaxis; D003176:Complement C3 / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism*; D004789:Enzyme Activation; D006098:Granulocytes / Q000276:immunology; D006146:Guanidines; D006168:Guinea Pigs; D006801:Humans; D007104:Immune Adherence Reaction; D013211:Staphylococcus aureus / Q000276:immunology* / Q000472:pathogenicity
D003176:Complement C3; D006146:Guanidines; D003165:Complement System Proteins
Untersuchungen über die Komplementaktivierung bekapselter und unbekapselter Staphylokokken nach Extraktion mit Guanidinium-chlorid