Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity.
The application of roller-bottle cell culture techniques and a relatively simple purification scheme has led to the isolation of milligram quantities of a polypeptide cell multiplication stimulating activity (MSA) from Buffalo rat liver cell conditioned medium. We have characterized the apparently homogeneous MSA with respect to its biological activity, its N-terminal amino acid residue, and its amino acid composition, and have tested the MSA for growth-promoting activity in a number of cell types.
D000596:Amino Acids / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006133:Growth Substances / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology
D000596:Amino Acids; D003470:Culture Media; D006133:Growth Substances