Gas chromatographic separation of diastereoisomeric amino acid derivatives on glass capillaries. THE USE OF PENTAFLUOROPROPIONYL-AMINO ACID (+)-3-METHYL-2-BUTYL ESTERS.
The separation of diastereoisomeric pentafluoropropionyl-D, L-amino acid (+)-3-methyl-2-butyl esters, including those derived from histidine, arginine and tryptophan, is achieved on glass capillaries. Separation factors of between 1.03 and 1.09 are observed for the derivatives of protein amino acids. The gas chromatographic separation of a mixture of common amino acids usually takes less than 1 h.
D000596:Amino Acids / Q000032:analysis*; D000613:Aminobutyrates / Q000032:analysis; D001120:Arginine / Q000032:analysis; D002849:Chromatography, Gas; D005459:Fluorides / Q000032:analysis; D005898:Glass; D006639:Histidine / Q000032:analysis; D008745:Methylation; D011422:Propionates / Q000032:analysis; D013237:Stereoisomerism; D014364:Tryptophan / Q000032:analysis
D000596:Amino Acids; D000613:Aminobutyrates; D011422:Propionates; D006639:Histidine; D014364:Tryptophan; D001120:Arginine; D005459:Fluorides