Transfection of Streptococcus sanguis by phage deoxyribonucleic acid isolated from Streptococcus mutans.
Streptococcus sanguis ATCC 10556 cells were infected with free phage DNA of S, mutans strain PK 1. Two transformants were isolated which made colonies with large mucoid forms on mitis-salivarius agar. Both transformants had an increased ability to synthesize insoluble glucan and showed an adhesive nature on glass surfaces. These characteristics of the transformants bear a resemblance to S. mutans. These transformants had many physiological characteristics by which they could be recognized as S. sanguis. However, they resembled S. salivarius in forming a large amount of soluble fructan. Furthermore, the transformant cells did not produce ammonia from arginine, whereas their parent cells did.
D001433:Bacteriolysis / Q000187:drug effects; D001435:Bacteriophages; D002448:Cell Adhesion; D004279:DNA, Viral; D005898:Glass; D005998:Glycine / Q000096:biosynthesis; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D013291:Streptococcus; D013295:Streptococcus mutans; D013298:Streptococcus sanguis; D014170:Transformation, Genetic
D004279:DNA, Viral; D008937:Mitomycins; D011135:Polysaccharides, Bacterial; D005998:Glycine