title: Identification of glucuronides as in vivo liver conjugates of seven cannabinoids and some of their hydroxy and acid metabolites.
abstract: Glucuronide conjugates of cannabidiol (CBD), 7-hydroxy-CBD, propyl-CBD, cannabinol (CBN), 7-hydroxy-CBN, CBN-7-oic acid, propyl CBN and cannabichromene have been identified as major metabolites of CBD, CBN and their propyl homologues and of cannabichromene in mouse liver. Trace amounts of the glucuronide conjugates of delta1- and delta1(6)-tetrahydrocannabinol (THC) were also detected. Identification was made by combined gas-liquid chromatographic and mass spectrometric studies of the trimethylsilyl (TMS), d9-TMS and methyl ester-TMS derivatives of the glucuronides and the TMS derivatives of the product of the reduction of the metabolites with lithium aluminium deuteride.
mesh_terms: D000818:Animals; D002185:Cannabidiol / Q000378:metabolism; D002186:Cannabinoids / Q000032:analysis / Q000378:metabolism*; D002187:Cannabinol / Q000378:metabolism; D002188:Cannabis; D002849:Chromatography, Gas; D013759:Dronabinol / Q000378:metabolism; D005965:Glucuronates / Q000096:biosynthesis*; D006900:Hydroxylation; D008099:Liver / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D051379:Mice
chemical_list: D002186:Cannabinoids; D005965:Glucuronates; D002185:Cannabidiol; D013759:Dronabinol; D002187:Cannabinol