Genetic determination of methylenomycin synthesis by the SCP1 plasmid of Streptomyces coelicolor A3(2).
Evidence is presented that genes determining the pathway of methylenomycin A synthesis are carried on the SCP1 plasmid. All 16 mutations (mmy) leading to lack of antibiotic synthesis were SCP1-linked. Phenotypic classification, by co-synthesis and other criteria, suggested that they fell into at least five classes. When the wild-type SCP1 plasmid was transferred to Streptomyces lividans or Streptomyces parvulus, material that was chromatographically and biologically indistinguishable from methylenomycin A was produced. Recombination between some pairs of mmy mutations was detected. In crosses of mmy mutants of NF (integrated SCP1 donor) strains with SCP1-, a very high frequency of chromosomal recombination occurred; thus methylenomycin production appears not to be an important cause of the ultra-fertility normally associated with NF X SCP1- crosses.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D004885:Erwinia / Q000187:drug effects; D005111:Extrachromosomal Inheritance; D005796:Genes; D009154:Mutation; D010455:Peptides; D010641:Phenotype; D010957:Plasmids; D011995:Recombination, Genetic; D013302:Streptomyces / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D010455:Peptides; C014097:methylenomycin A