Effects of inosine on purine synthesis in normal and HGPRT-deficient human fibroblasts.
1. Incubation of normal and HGPRT-deficient fibroblasts with inosine results in increased PP-ribose-P concentrations. 2. The increased PP-ribose-P concentrations are accompanied by decreased rates of purine synthesis de novo, more marked in normal cells 3. Increased purine nucleotide concentrations during incubation with inosine provide a likely explanation for the inhibition of purine synthesis in normal cells 4. The lack of accelerated purine synthesis in mutant cells under these conditions is not fully explained by consideration of PP-ribose-P and purine nucleotide concentations.
D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency*; D007288:Inosine / Q000494:pharmacology*; D007926:Lesch-Nyhan Syndrome / Q000378:metabolism; D008751:Methylene Blue / Q000494:pharmacology; D009154:Mutation; D010428:Pentosephosphates / Q000378:metabolism*; D010754:Phosphoribosyl Pyrophosphate / Q000378:metabolism*; D011685:Purine Nucleotides / Q000378:metabolism; D011687:Purines / Q000096:biosynthesis*; D012267:Ribosemonophosphates / Q000378:metabolism
D010428:Pentosephosphates; D011685:Purine Nucleotides; D011687:Purines; D012267:Ribosemonophosphates; D007288:Inosine; D010754:Phosphoribosyl Pyrophosphate; D007041:Hypoxanthine Phosphoribosyltransferase; D008751:Methylene Blue