A 51chromium isotope release assay for detecting cytotoxicity to human bladder carcinoma.
A 51chromium isotope release assay is described for measuring in vitro cytotoxicity reactions against allogenetic target cells. Lymphoid cells from some patients with localized transitional cell carcinoma (TCC) were shown to selectively lyse targets derived from two long-term cell lines of TCC. The effector cells in this reaction did not form E rosettes. Cytotoxicity was successfully recovered after cryopreservation.
D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D002452:Cell Count; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D005615:Freezing; D006801:Humans; D007104:Immune Adherence Reaction; D007111:Immunity, Cellular; D008214:Lymphocytes / Q000276:immunology; D013997:Time Factors; D014021:Tissue Preservation; D001743:Urinary Bladder / Q000166:cytology; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D002860:Chromium Radioisotopes