Identification of drugs, drug metabolites, and other compounds in urine by permethylation and gas-phase analysis.
A small aliquot of urine (50-200 mul) is evaporated to dryness, the residue permethylated with the methylsulfinylmethide carbanion and methyl iodide, and the product mixture separated and analyzed by GC and GC-MS. Certain drugs (especially the anticonvulsants), drug metabolites, mono-, di-, and trisaccharides, and organic acids, including fatty acids and glucuronides, can be identified in the mixture. The technique is probably useful mainly as a screening method for the detection of any of these types of compounds in urine.
D000293:Adolescent; D000328:Adult; D000596:Amino Acids / Q000652:urine; D000927:Anticonvulsants / Q000652:urine; D002241:Carbohydrates / Q000652:urine; D002784:Cholesterol / Q000652:urine; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000652:urine; D005260:Female; D005965:Glucuronates / Q000652:urine; D006801:Humans; D006914:Hydroxysteroids / Q000652:urine; D008297:Male; D013058:Mass Spectrometry; D008722:Methods; D008745:Methylation; D009705:Nucleosides / Q000652:urine; D010455:Peptides / Q000652:urine; D004364:Pharmaceutical Preparations / Q000652:urine*; D011247:Pregnancy; D013997:Time Factors
D000596:Amino Acids; D000927:Anticonvulsants; D002241:Carbohydrates; D005227:Fatty Acids; D005965:Glucuronates; D006914:Hydroxysteroids; D009705:Nucleosides; D010455:Peptides; D004364:Pharmaceutical Preparations; D002784:Cholesterol