[Isolation of purified aspergillopepsin A].
Two methods of isolation of pure aspergillopepsin A have been developed. The first method is based on sequential chromatography of a crude preparation on gramicidin C-sepharose 4B and ECTEOLA-cellulose (35% yield, 100-fold purification). The second method consists in sequential chromatography of evaporated extract of Asp. awamori surface culture on Acrylex P-10, aminosilochrome and ECTEOLA-cellulose (40% yield, 430-fold purification). The methods discussed may be used to isolate pure aspergillopepsin A preparations in order to establish the primary structure of the enzyme.
D001230:Aspergillus / Q000201:enzymology*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004589:Electrophoresis, Disc; D010450:Endopeptidases / Q000302:isolation & purification*; D008722:Methods
D010450:Endopeptidases
Poluchenie ochishchennogo aspergillopepsina A