Acetaminophen in serum and plasma estimated by high-pressure liquid chromatography: a micro-scale method.
We describe a capillary-sampling method for serum or plasma acetaminophen by cation-exchange chromatography. As little as 1.5 mul of plasma or serum and an equal volume of the internal standard (N-butyryl-p-aminophenol) were run, with a precision of +/- 5% between duplicates. Acetaminophen and the internal standard chromatographed in 32 and 50 min, respectively, distinct from intrinsic plasma peaks and peaks caused by other medications.
D000082:Acetaminophen / Q000097:blood*; D002648:Child; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008722:Methods; D008832:Microchemistry
D000082:Acetaminophen