title: High multiplicity of H1 histones from mouse mammary tumors.
abstract: Lysine-rich (H1) histones were isolated from mammary tumors of GR mice. Chromatography of the H1 histone class on a long column (130 X 1 cm) of Amberlite IRC-50 resin revealed the presence of nine components, designated 1-9 according to their order of elution. Amino acid analysis indicated that components 2-9 represented H1 class histones, but that peak 1 represented a nonhistone protein. Studies of three types of mouse mammary tumors, viz. hormone-dependent, hormone-responsive and hormone-independent, did not reveal significant differences in elution profiles of the H1 histones. In contrast, the H1 histone class from a dog mammary tumor differed chromatographically from that of mouse mammary tumors, indicating species specificity in elution patterns of mammary tumor H1 histones.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002852:Chromatography, Ion Exchange; D004970:Estrone / Q000378:metabolism; D005260:Female; D006657:Histones / Q000032:analysis* / Q000302:isolation & purification; D008239:Lysine / Q000032:analysis; D008325:Mammary Neoplasms, Experimental / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D011374:Progesterone / Q000378:metabolism
chemical_list: D000596:Amino Acids; D006657:Histones; D004970:Estrone; D011374:Progesterone; D008239:Lysine