High-performance liquid chromatographic determination of pyruvic acid and alpha-ketoglutaric acid in serum.
A high-performance liquid chromatographic determination of pyruvic acid and alpha-ketoglutaric acid in serum is described which is based on the formation of 2,4-dinitrophenylhydrazones. The syn-anti isomerization of the 2,4-dinitrophenylhydrazone of pyruvic acid is also discussed.
D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D007536:Isomerism; D007656:Ketoglutaric Acids / Q000097:blood*; D010659:Phenylhydrazines; D011773:Pyruvates / Q000097:blood*; D013997:Time Factors
D007656:Ketoglutaric Acids; D010659:Phenylhydrazines; D011773:Pyruvates