Electron microscopy of human melanosomes in unstained, fresh air-dried hair bulbs and their examination by electron probe microanalysis.
Electron microscopy of unstained, fresh air-dried spreads of plucked human hair bulbs revealed relatively well preserved melanocytes and dense storage granules of melanin. Energy dispersive X-ray microanalysis of melanosomes and premelanosomes from black facial hair bulbs disclosed high peaks for potassium and sulfur, intermediate peaks for magnesium and calcium, and low but distinct ones for phosphorus and chlorine. No peaks for magnesium were found in the central portion of mature melanosomes, but at their edges and in premelanosomes conspicuous peaks for this element were obtained.
D004577:Electron Probe Microanalysis; D006197:Hair; D006801:Humans; D008544:Melanocytes / Q000032:analysis* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009940:Organoids / Q000032:analysis / Q000648:ultrastructure