Isolation and separation of inositol 1-phosphate, cyclic inositol 1,2-phosphate, and glycerylphosphoinositol from tissue culture cells labeled with [3H]inositol.
Water-soluble phosphorylated inositol derivatives have been separated from each other and from inositol by ion exchange and by thin-layer chromatography. The method, in combination with radioactive labeling, is sensitive enough to be applied to single tissue culture dishes corresponding to 10(6) - 10(7) cells. With this technique glycerylphosphoinositol, inositol 1-phosphate, cyclic inositol 1,2-phosphate, and at least three other inositol labeled compounds can be detected in human, monkey, hamster, mouse, and Japanese quail fibroblasts. During a 24-h incubation with [3H]inositol the extent of labeling of these compounds differed according to the cell type.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D003370:Coturnix; D005347:Fibroblasts / Q000378:metabolism; D005994:Glycerophosphates / Q000096:biosynthesis / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D007294:Inositol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D007739:L Cells / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000302:isolation & purification / Q000378:metabolism; D011784:Quail
D005994:Glycerophosphates; D009943:Organophosphorus Compounds; D007294:Inositol