title: New method for determining eye anesthesia in an animal model.
abstract: Some chemical which are injurious to the eye may also cause anesthesia. If the eye were unknowingly anesthetized, exposure to an irritant could go undected and cause injury. Techniques for determining whether the eye was anesthetized have been generally unreliable. Usually the technique consists of challenging the cornea with a probe and testing for a blink response. In a new method described herein, an indwelling subpalpebral lavage apparatus was surgically implanted in the dog. Through this apparatus, a test material was instilled into the eye without the animal's anticipation. Responses caused by the materials were monitored by electroencephalography. The normal response to an irritting material was increased frequency and decreased amplitude of the electroencephalogram tracing or a deflection of thepolygraph needle (blink response), or both. The method was evaluated with known eye anesthetic agents and appeared to be a useful way of detecting eye anesthesia.
mesh_terms: D000772:Anesthesia, Local / Q000379:methods / Q000662:veterinary*; D000818:Animals; D004285:Dogs / Q000502:physiology*; D004569:Electroencephalography; D005123:Eye / Q000187:drug effects*; D008954:Models, Biological; D009799:Ocular Physiological Phenomena; D011430:Propoxycaine; D012965:Sodium Chloride / Q000494:pharmacology; D013748:Tetracaine; D007507:Therapeutic Irrigation / Q000295:instrumentation
chemical_list: D013748:Tetracaine; D012965:Sodium Chloride; D011430:Propoxycaine