Uptake of pyridoxine hydrochloride by the rat jejunal mucosa in vitro.
The kinetics of mucosal membrane transport of pyridoxine hydrochloride were evaluated in vitro in the rat jejunum. Utilizing everted sacs and a double-label isotope technique, short-term incubation within the period of initial linear tissue uptake indicated: 1) no evidence of saturation of uptake over a wide pyridoxine-HCl concentration range (0.01 muM-10 mM); 2) failure of 4-deoxypyridoxine (10 muM), anoxia, iodoacetamide (5 mM), Na+ replacement and ouabain (1 mM) to inhibit uptake of 2 muM pyridoxine-HCl significantly; and 3) a low Q10 value of 1.31. Using single-label techniques, sacs were also incubated for 1 hour in 2 muM pyridoxine HCl with determination of the apparent tissue water-mucosal fluid concentration ratio and chromatographic separation of the various forms of vitamin B6 in tissue. Results demonstrated a failure of pyridoxine in tissue water to achieve a concentration in excess of that in the incubation medium. Data, therefore, were most consistent with passive diffusion as the mechanism for in vitro jejunal mucosal uptake of pyridoxine-HCl in the rat.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D000860:Hypoxia / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D007460:Iodoacetamide / Q000494:pharmacology; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D011736:Pyridoxine / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000494:pharmacology
D010042:Ouabain; D012964:Sodium; D011736:Pyridoxine; D007460:Iodoacetamide