High molecular weight virion-associated RNA of vaccinia. A possible precursor to 8 to 12 S mRNA.
The high molecular weight virion-associated RNA synthesized by vaccinia in vitro can be cleaved into smaller components, some of which are extruded from the virus as 8 to 12 S RNA. The high molecular weight virion-associated RNA fails to bind appreciably (5%) to poly(U) filters indicating that it is not polyadenylated. Its cleavage products will, however, bind to poly(U) (40 to 50%) after processing in the presence of ATP. The high molecular weight virion-associated RNA is methylated by the virus, and purified unmethylated RNA can be methylated by detergent-solubilized extracts of vaccinia virus cores. In the presence of GTP, methylation is stimulated 3-fold. The level of methylation of purified unmethylated high molecular weight RNA achieved by soluble core extracts is approximately 80% of the level of methylation achieved with purified unmethylated 8 to 12 S viral RNA, suggesting that more residues than the primary 5' termini became methylated. Approximately 85% of the methylated RNA binds to poly(U) when purified high molecular weight RNA is processed with soluble core extracts in the presence of S-adenosyl[methyl-3H]methionine, GTP, and ATP, conditions which also cleave the RNA. Nucleic acid hybridization-competition studies indicate that virion-extruded 8 to 12 S mRNA contains sequences found in the high molecular weight virion-associated RNA.
D002499:Centrifugation, Density Gradient; D007700:Kinetics; D008970:Molecular Weight; D011061:Poly A / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic; D014616:Vaccinia virus / Q000378:metabolism*; D012359:tRNA Methyltransferases / Q000378:metabolism
D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D012359:tRNA Methyltransferases