Picosecond laser study of fluorescence lifetimes in spinach chloroplast photosytem I and photosystem II preparations.
Fractions enriched in either Photosystem I or Photosystem II have been prepared from chloroplasts with digitonin. A more detailed analysis of the decay kinetics of fluorescence excited by a picosecond laser pulse has been possible compared to experiments with unfractionated systems. The Photosystem I fractions show a very short component (less than or equal to 100 ps) at room temperature which is apparently independent of pulse intensity over the range of photon densities used (5 - 10(13)--1 - 10(16) photons cm-2). The Photosystem II fraction has a short initial lifetime at room temperature which is strongly intensity-dependent approaching 500 ps at low photon densities, but decreasing to close to 150 ps at the highest photon densities. All of these room temperature decays appear to be non-exponential, and may possibly be fitted by at t1/2 expression, expected from a random diffusion of excitations via Förster energy transfer. On cooling to 77K, lifetimes of both Photosystem I and Photosytem II increase, the lengthening with Photosystem I being more striking. The Photosystem I decays become intensity dependent like the Photosystem II, and at the lowest photon densities decays which are more nearly exponential within the experimental error give initial lifetimes of about 2 ns. The non-exponential decays seen at high photon densities appear to fit a t1/2 expression.
D002736:Chloroplasts / Q000378:metabolism*; D004072:Digitonin; D007700:Kinetics; D007834:Lasers; D010785:Photophosphorylation; D010788:Photosynthesis; D010944:Plants; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors
D004072:Digitonin