Enzymatic assay of fructose-1,6-diphosphate for the measurement of its utilization by tissues.
The enzymatic analysis of fructose-1,6-diphosphate by a coupled assay utilizing triose-phosphate isomerase, glycerol-3-phosphate dehydrogenase and aldolase was carried out in rat serum and human plasma after fructose-1,6-diphosphate administration. The rate of disappearance of fructose-1,6-diphosphate was determined and compared to the activity of aspecific phosphatase. The uptake of fructose-1,6-diphosphate by rat liver and ileum was also determined with the same method.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005636:Fructosephosphates / Q000097:blood / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D007082:Ileum / Q000201:enzymology / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051381:Rats; D014305:Triose-Phosphate Isomerase / Q000378:metabolism
D005636:Fructosephosphates; D005993:Glycerolphosphate Dehydrogenase; D000469:Alkaline Phosphatase; D005634:Fructose-Bisphosphate Aldolase; D014305:Triose-Phosphate Isomerase