Determination of debrisoquine and its 4-hydroxy metabolite in biological fluids by gas chromatography with flame-ionization and nitrogen-selective detection.
Specific methods have been devised for the simultaneous determination of the guanidino-type antihypertensive agent debrisoquine and its 4-hydroxy metabolite in human urine, involving in situ derivatisation with acetylacetone, extraction of the resulting pyrimidines, and gas chromatography using a flame-ionisation detector or a nitrogen-specific detector. Using the latter, a similar procedure was also developed to measure debrisoquine in human plasma and whole blood following prior extraction at pH 13.5 with chloroform. Concentrations down to 3 ng/ml can be measured accurately using a 1.0-ml sample. The methods were applied to the analysis of samples collected after a single 20-mg oral dose of debrisoquine hemisulphate.
D002849:Chromatography, Gas; D003647:Debrisoquin / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine*; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D006801:Humans; D007546:Isoquinolines / Q000652:urine*
D007546:Isoquinolines; D003647:Debrisoquin