The role of heparin on platelet retention by acrylonitrile co-polymer dialysis membranes.
The role of heparin on platelet--foreign surface interactions was examined by platelet retention studies on acrylonitrile--dimethylaminoethyl methacrylate (AN-DMAEMA) dialysis membranes both with and without the bonding of heparin onto their surfaces. Heparin bonding significantly reduced platelet retention. Heparin in solution (4 units/ml.) increased platelet retention when the surface of the membranes was modified by ethylene oxide but had no significant effect on the platelet-retaining properties of unmodified membranes. Studies using heparin 99mTc demonstrated that unmodified membranes took up heparin from solution whereas ethylene oxide-modified membranes had little such affinity. The heparin bonding process greatly increased the heparin uptake achieved by simple soaking in heparin solution, and the leaching rate was less than 1% at 70 hours. The results indicate that heparin has two antagonistic effects in this platelet-foreign surface interaction: it acts directly on platelets to increase adhesiveness while acting on the foreign surface to reduce platelet retention.
D000179:Acrylates; D000181:Acrylonitrile; D001792:Blood Platelets / Q000378:metabolism*; D005027:Ethylene Oxide; D006493:Heparin / Q000494:pharmacology*; D008567:Membranes, Artificial; D008689:Methacrylates; D009570:Nitriles; D010973:Platelet Adhesiveness / Q000187:drug effects
D000179:Acrylates; D008567:Membranes, Artificial; D008689:Methacrylates; D009570:Nitriles; D006493:Heparin; D005027:Ethylene Oxide; D000181:Acrylonitrile