title: Isolation of prolactin from human amniotic fluid.
abstract: Amniotic fluid, obtained from women at term, was centrifuged and concentrated by ultrafiltration. The concentrated amniotic fluid was purified by gel filtration on Sephadex G-100 and ion-exchanged chromatography on DEAE-cellulose. The fractions obtained during purification procedures were assayed for prolactin and somatotropin activities by respective radioimmunoassays. The prolactin-rich fraction obtained from ion-exchange chromatography was further purified by isoelectric focusing. A yield of 0.21 mg of highly pruified prolactin containing 40 units/mg was obtained from 1 liter of the amniotic fluid. The prolactin was free of somatotropin and human placental lactogen.
mesh_terms: D000653:Amniotic Fluid / Q000032:analysis*; D005260:Female; D013006:Growth Hormone / Q000032:analysis; D006801:Humans; D010928:Placental Lactogen / Q000032:analysis; D011247:Pregnancy; D011388:Prolactin / Q000302:isolation & purification*; D011863:Radioimmunoassay
chemical_list: D011388:Prolactin; D013006:Growth Hormone; D010928:Placental Lactogen