Poly(A)-containing RNA in neuroblastoma: immature and differentiated cells in culture.
We have analysed the poly(A)-containing RNA from neuroblastoma cells at two different developmental states: either as round, immature neuroblasts grown in suspension, or as differentiated cells exhibiting the morphological properties of mature neurons, when attached to a culture dish. Suspension-grown and monolayer cells were pulse-labelled with tritiated uridine. The profile of cytoplasmic poly(A)-containing RNA from suspension cells is highly heterogeneous with peaks ranging from 16-30 S. The profile obtained from differentiated cells appears somewhat distinct from the previous one. This is evidenced by a relative decrease in the 26-S peak and a virtual disappearance of the 16-S component. In order to compare the 'steady-state' patterns of poly(A)-containing RNA in these two developmental stages, polysomal RNA was prepared from unlabelled cells. Following sucrose gradient sedimentation, each fraction was hybridized to [3H]poly(U). Examination of the two RNA hybridization profiles reveals striking similarities suggesting that 'steady-state' messenger populations include, on the average, the same subspecies. The 16-S fraction, which was not observed after the pulse-labelling of the monolayer culture, is detected here by hybridization to [3H]poly(U) when using polysomal poly(A)-containing RNA from monolayer cells as substrate. These results suggest that terminal differentiation of neuroblastoma cells is not accompanied by major alterations of the transcription program and is paralleled by a marked stabilization of the 16-S species.
D002454:Cell Differentiation; D002460:Cell Line; D003593:Cytoplasm / Q000032:analysis; D009447:Neuroblastoma / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis*; D011072:Poly U; D011132:Polyribosomes / Q000032:analysis; D012334:RNA, Neoplasm / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism
D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D011061:Poly A; D011072:Poly U