Replication process of the parvovirus H-1. VIII. Partial denaturation mapping and localization of the replication origin of H-1 replicative-form DNA with electron microscopy.
Partial denaturation mapping, restriction endonuclease digestion, and electron microscopy were used to determine which end of the linear duplex replicative-form (RF) DNA molecule contains the origin of RF replication for the parvovirus H-1. This origin was localized within approximately 300 base pairs of the arbitrarily designated right end of the RF DNA, in the EcoRI or HaeII-A fragment. Based on denaturation behavior in formamide, the right end was also found to have a relatively high guanine plus cytosine content, whereas the region adjacent to the left terminus of the RF DNA molecule was adenine plus thymine rich.
D004261:DNA Replication; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D008854:Microscopy, Electron; D009691:Nucleic Acid Denaturation; D009705:Nucleosides / Q000032:analysis; D010321:Parvoviridae / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D014779:Virus Replication
D004279:DNA, Viral; D009705:Nucleosides; D004262:DNA Restriction Enzymes