Detection of anti-red blood cell antibodies by treatment of the cells with neuraminidase.
Neuraminidase treated RBC were used to detect incomplete unbound anti RBC antibodies in the plasma of patients with immune diseases. 80% (48 out of 60) of the examined patients gave positive results with this method while all the 50 normal controls were negative. The indirect antiglobulin test was negative in all the patients. In the direct antiglobulin test only two RBC samples, whether treated with neuraminidase or not, gave positive results. The present study indicates that the assay of plasma on neuraminidase treated RBC may serve as a sensitive method for the detection of the presence of anti RBC antibodies in various immunological diseases.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000372:Agglutination Tests; D000906:Antibodies / Q000032:analysis; D001327:Autoimmune Diseases; D003298:Coombs Test; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009439:Neuraminidase / Q000494:pharmacology*; D010206:Papain / Q000494:pharmacology
D000906:Antibodies; D009439:Neuraminidase; D010206:Papain