Effect of hyperosmolarity on resting and developed tension in heart muscle.
Simultaneous changes in weight, tension, and electrical activity were studied in the isolated perfused rabbit heart when the Ringer solution perfusion fluid was made hypertonic by the addition of sucrose, urea, glycerol, ethylene glycol, or formamide. The typical responses to each of the molecules was an initial drop in weight and tension followed by a return toward the base-line level. A 0.4 M concentration of sucrose, urea, or glycerol reduced the weight to 40 +/- 4.6, 48 +/- 3, and 52.2 +/- 3% of the initial value, respectively. The tension was simultaneously reduced to 23 +/- 3.5, 31 +/- 2, and 41 +/- 4% of its initial value. The tension drop produced by the solutes tested was linearly related to the amount of water lost by the heart. The falling phases of both tension and weight loss were closely correlated phases of both tension and weight loss were closely correlated in time a magnitude and were both related to the effectiveness of a particular molecule to move water out of the cells. In contrast, the subsequent rising phases of tension and weight were not as well correlated in time and magnitude. Whereas for most of the molecules used, the tension recovery was incomplete; urea, in contrast, caused an overshoot of the control tension level, thus pointing toward a unique inotropic effect of this compound. Resting tension rose for both urea and sucrose but not for the other compounds.
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D004562:Electrocardiography; D005026:Ethylene Glycols / Q000494:pharmacology; D005260:Female; D005559:Formamides / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D006321:Heart / Q000033:anatomy & histology; D007552:Isotonic Solutions; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D009994:Osmolar Concentration; D010477:Perfusion; D011817:Rabbits; D013395:Sucrose / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology; D014867:Water
D005026:Ethylene Glycols; D005559:Formamides; D007552:Isotonic Solutions; D014867:Water; D013395:Sucrose; D014508:Urea; D005990:Glycerol; D002118:Calcium