Separation of quinones and their derivatives by high-performance liquid chromatography.
The separation of mixtures of quinones by high-performance liquid chromatography has been studied. The technique described is suitable for separating differently substituted quinones. Particularly convenient is the conversion of quinones into 2,4-dinitrophenylhydrazones, which makes the reliable identification of benzoquinones and naphthoquinones possible. Studies of the separation of 2,4-dinitrophenylhydrazones of quinones and nitrophenols on Sephadex LH-20 gel indicated that the substances in question were not separated on the basis of differences in molecular weight; but the RF values were proportional to the dissociation constant.
D000880:Anthraquinones / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006835:Hydrazones / Q000302:isolation & purification; D008722:Methods; D009285:Naphthoquinones / Q000302:isolation & purification; D011809:Quinones / Q000302:isolation & purification*
D000880:Anthraquinones; D006835:Hydrazones; D009285:Naphthoquinones; D011809:Quinones