title: Fluid reabsorption in the isolated duct of the rat cauda epididymidis.
abstract: A technique is described fro measurement of rate of fluid reabsorption in a segment of the rat cauda epididymidis in vitro. The basal rate was 2-63 +/- 0-22 mul/cm2/30 min and was dependent on temperature and inhibited by 2,4-dinitrophenol, suggesting that fluid reabsorption is an energy-dependent process. About 50% of the fluid reabsorption was dependent on intraluminal sodium ions. This Na+-dependent component was inhibited by addition of amiloride to the intraluminal fluid and ouabain to the peritubular fluid.
mesh_terms: D000042:Absorption; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D012661:Semen / Q000378:metabolism; D012964:Sodium / Q000494:pharmacology; D013696:Temperature
chemical_list: D001639:Bicarbonates; D004140:Dinitrophenols; D000584:Amiloride; D012964:Sodium; D011188:Potassium