[Kinetics and properties of a cortisol-resistant population of lymphocytes from lymph nodes of guinea pigs sensitized to protein].
Lymphocytes from the cervical lymph nodes of guinea pigs were incubated in medium 199 in the presence of cortizol in a concentration of 20 and 100 microng% for 24 hours. A determination was made of the lymphocyte survival and cortizol metabolism by them, and also (cytophotometrically) the nucleic acid content. In the intact and the sesitized guinea pigs progesterone in a concentration of 10-5 inhibited both the lytic action of coritizol and its metabolism There was observed a marked reduction of cortizol meta bolism by the lymphocytes from the 6th day after the sensitization, which was restored only by the 90th day. The cortizol-resistant lymphocyte population increased on the 17th--30th day. In a concentration of 100 microng% cortizol reduced the nucleic acid content in the lymphocytes of both the intact guinea pigs and sensitized ones on the 17th--30th day, when the cortizol-resistant population increased.
D000818:Animals; D001798:Blood Proteins / Q000276:immunology; D004347:Drug Interactions; D006168:Guinea Pigs; D006854:Hydrocortisone / Q000378:metabolism / Q000494:pharmacology*; D006967:Hypersensitivity / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008297:Male; D011374:Progesterone / Q000494:pharmacology; D013997:Time Factors
D001798:Blood Proteins; D011374:Progesterone; D006854:Hydrocortisone
Kinetika i svoĭstva kortizol-rezistentnosti populiatsii limfotsitov iz limfaticheskikh uzlov morskikh svinok pri belkovoĭ sensibilizatsii