title: Spatial distribution of DNA-synthesizing cells in colonic crypts of the guinea pig.
abstract: The ascending colon of a guinea pig injected with tritiated thymidine was cut serially, autoradiographed and stained with periodic-acid Schiff-hematoxylin. Maps of transversely sectioned crypts were prepared with the use of a microscope eye-piece projector. The number and angular positions of pulselabelled (DNA-synthesizing) cells around the circumference of transverse sections of the crypt were recorded. A method of 'statistics of the circumference' was applied in order to find the variances of angular distances between labelled cells and thereby to find the type of arrangement of DNA-synthesizinbg cells in the crypt. The spatial distribution of DNA-synthesizing cells both around the crypt circumference and along the crypt, was found to be non-random. While the pattern of non-randommess around the crypt circumference is such that the DNA-synthesizing cells tend to occupy positions in the crypt circumference at maximal distances from each other, DNA-synthesizing cells along the crypt tend to occupy positions at minimal distances from each other. DNA-synthesizing cells are arranged in the crypt in rows, each consisting of several cells and each parallel to the long axis of the crypt. Apparently the dividing cell of the crypt produces either two proliferating or two differentiating cells. No evidence of differential mitosis could be found.
mesh_terms: D000818:Animals; D003106:Colon / Q000166:cytology* / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D006168:Guinea Pigs; D008297:Male
chemical_list: D004247:DNA