Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism. VI. Enzymatic studies of two mutants unable to convert inosinic acid to adenylic acid.
Ade-H and ade-I are two auxotrophic mutants of Chinese hamster ovary (CHO-K1) cells which specifically require adenine as the purine source to grow. The enzymatic defects of these mutants were examined in cell-free extracts. It was found that ade-H did not have any detectable adenylosuccinate synthetase activity and ade-I was defective in the adenylosuccinate lyase enzyme. The relevance of adenine-requiring mutants to the study of the regulation of purine metabolism in mammalian cells is discussed.
D000225:Adenine / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism; D000642:Ammonia-Lyases / Q000378:metabolism*; D002460:Cell Line; D007291:Inosine Monophosphate / Q000378:metabolism; D009154:Mutation; D010453:Peptide Synthases / Q000378:metabolism*
D007291:Inosine Monophosphate; D000249:Adenosine Monophosphate; D000642:Ammonia-Lyases; D010453:Peptide Synthases; D000225:Adenine