title: Inhibition of DNA synthesis by cycloheximide and blasticidin-S is independent of their effect on protein synthesis.
abstract: The effects of cycloheximide and related glutarimide antibiotics on DNA synthesis in Achlya bisexualis Coker and A. Couch were compared to those of other protein synthesis inhibitors, puromycin, p-fluorophenylalanine and blasticidin-S. The inhibitors had no significant effects on intrahyphal pool sizes of dTTP, dCTP, ATP, UTP and CTP, nor on the specific activity of the dTTP pool labelled by [3H] thymidine. DNA was the sole acid-insoluble product of [3H]-thymidine incorporation. Cycloheximide, isocycloheximide, streptimidone and blasticidin-S inhibited DNA synthesis rapidly and completely and anhydrocycloheximide was less effective. Cycloheximide acetate, puromycin and p-fluorophenylalanine did not inhibit DNA synthesis. It is concluded that the effects of the several glutarimide antibiotics and of blasticidin-S on DNA synthesis were independent of their effects on protein synthesis.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects*; D005658:Fungi / Q000187:drug effects / Q000378:metabolism*; D006146:Guanidines / Q000494:pharmacology; D007700:Kinetics; D014176:Protein Biosynthesis / Q000187:drug effects*; D013329:Structure-Activity Relationship; D013936:Thymidine / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D006146:Guanidines; D003513:Cycloheximide; D013936:Thymidine