title: Quantitative determination of creatine kinase isoenzyme catalytic concentrations in serum using immunological methods.
abstract: For the determination of creatine kinase isoenzyme catalytic concentrations in serum two methods based on immunological reactions are presented: One method uses inhibiting antibodies, which selectively block the activity of creatine kinase M subunits ("Inhibition Test"). This test is used for routine measurements of creatine kinase MB catalytic concentration; Another method uses precipitating antibodies, which allows a quantitative differentiation of creatine kinase isoenzymes MM, MB and BB ("Precipitation Test"). This test is used as a control for the Inhibition Test for the possible presence of creatine kinase BB activities in doubtful cases. Procedures, specificity, correlation and application of these methods are discussed.
mesh_terms: D003402:Creatine Kinase / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007527:Isoenzymes / Q000097:blood*; D011233:Precipitin Tests / Q000379:methods
chemical_list: D007527:Isoenzymes; D003402:Creatine Kinase