Effect of type and amount of dietary fat and 1,2-dimethylhydrazine on biliary bile acids, fecal bile acids, and neutral sterols in rats.
The effect of type (corn oil or lard) and quantity (5 or 20%) of dietary fat and 1,2-dimethylhydrazine (DMH) on the composition of biliary bile acids, fecal bile acids, and neutral sterols was studied in rats exposed to a given regimen for two generations prior to s.c. treatment with DMH for 20 weeks. Biliary excretion of total bile acids as well as cholic acid, beta-muricholic acid, ursodeoxycholic acid, and deoxycholic acid was higher in rats fed a diet containing 20% corn oil or lard than it was in rats fed diets containing 5% corn oil or lard. Treatment of animals with DMH produced an increase in biliary total bile acids, cholic acid, hyodeoxycholic acid, and deoxycholic acid irrespective of diets. High-fat (corn oil or lard at 20% level) intake was associated with an increased excretion of fecal neutral sterols and bile acids. The excretion of deoxycholic acid, lithocholic acid, and 12-ketolithocholic acid was increased in rats fed high-fat diets. The source of fat had no major influence on the excretory pattern of cholesterol metabolites and bile acids. DMH-treated animals excreted higher levels of fecal coprostanol, coprostanone, deoxycholic acid, lithocholic acid, and 12-ketolithocholic acid than did controls.
D000818:Animals; D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D002793:Cholic Acids / Q000378:metabolism; D003110:Colonic Neoplasms / Q000209:etiology; D003840:Deoxycholic Acid / Q000378:metabolism; D004041:Dietary Fats / Q000009:adverse effects; D004127:Dimethylhydrazines / Q000494:pharmacology*; D005243:Feces / Q000032:analysis; D005260:Female; D006834:Hydrazines / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009374:Neoplasms, Experimental / Q000209:etiology; D009821:Oils; D051381:Rats; D011916:Rats, Inbred F344; D013261:Sterols / Q000378:metabolism*; D003313:Zea mays
D001647:Bile Acids and Salts; D002793:Cholic Acids; D004041:Dietary Fats; D004127:Dimethylhydrazines; D006834:Hydrazines; D009821:Oils; D013261:Sterols; D003840:Deoxycholic Acid; D002784:Cholesterol