Role of depolarization in acetylcholine-induced contractions of dog trachealis muscle.
The effects of acetylcholine (ACh) and K+ on tension and membrane potential were studied in order to determine the role of electromechanical and pharmacomechanical coupling in ACh-induced contractions of dog trachealis muscle. Potassium causes sustained contractions of the muscle via membrane depolarization, which appears to be related in a linear manner to the log of the external K+ concentration. From the K+ experiments a curve relating membrane potential and tension was obtained. The resting membrane potential of the trachealis is -30 +/- 1 mV. At a dose of 10(-7) M ACh the membrane is depolarized to about -55 mV and then at 10(-6) M ACh there is more depolarization to about -40 mV. At higher doses of drug there is no further change in membrane potential. The relationship between the dose of ACh and tension is much different. Contraction begins at 10(-7) M ACh but does not saturate until a dose of 10(-3) M is reached. The data were interpreted by using the relationship between EM and tension from the K+ experiments and the ACh dose-response curves as follows. At 10(-7) M ACh only pharmacomechanical coupling is involved in the contraction. At higher drug doses both pharmaco- and electromechanical coupling are involved, but pharmacomechanical coupling appears to play a much greater role. The maximal contribution of electromechanical coupling is approximately 30% of the contraction and this occurs at 10(-6) M ACh.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D004285:Dogs; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects*; D008839:Microelectrodes; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D011188:Potassium / Q000494:pharmacology
D000109:Acetylcholine; D011188:Potassium