Antibody-mediated suppression of tumor growth. III. Molecular assay of murine IgG1 alloantibody required to cause tumor suppression in vivo.
A radioimmunoassay was used to quantitate the number of tumor cell-bound IgG1 anti-tumor antibody molecules required to cause suppression of tumor growth in C3H mice. Radiolabeled anti-IgG1 was used to detect cell-bound IgG1 antibody. The assay was calibrated by using TNP-coupled tumor cells that had a known number of bound IgG1 anti DNP molecules. Between 70,000 and 130,000 IgG1 anti-tumor antibody molecules per tumor cell were sufficient to cause 50% suppression of tumor growth in mice inoculated with appriximately 50,000 tumor cells.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002471:Cell Transformation, Neoplastic; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis*; D007166:Immunosuppressive Agents; D007518:Isoantibodies / Q000032:analysis*; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay
D000912:Antibodies, Neoplasm; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D007518:Isoantibodies