Microbial models of mammalian metabolism O-demethylations of papaverine.
1. Papaverine is converted to its 4'-, 6- and 7-desmethyl metabolites when incubated with hepatic microsomal preparations from phenobarbital-induced rats. The 7-desmethyl compound was predominantly formed in these studes. When incubated with guinea-pig microsomal preparations, papaverine was converted to its 6- and 4'-desmethyl metabolites in approximately equal amounts. 2. The O-demethylation of papaverine was studied with sixty micro-organisms. Ten organisms (including Aspergillus Cunninghamella and Streptomyces species) were found to actively metabolize this drug in a manner similar to that of mammals. 3. A strain of Aspergillus alliaceus was used to produce gram quantitites of the mammalian metabolite, 6-desmethylpapaverine. Preparative amounts of 4'-desmethylpapaverine, a major mammalian metabolite of papaverine in vivo, was produced with a strain of Cunninghamella echinulata.
D000818:Animals; D003640:Dealkylation; D005658:Fungi / Q000378:metabolism; D006168:Guinea Pigs; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D008954:Models, Biological; D010208:Papaverine / Q000378:metabolism*; D051381:Rats
D010208:Papaverine