Fluorimetric determination of plasma unesterified fatty acid.
A reliable and simple method is described for the determination of plasma unesterified fatty acid. The method involves extraction by Dole's reagent, washing with dilute sulphuric acid and determination by the decrease of fluorescence of a dilute solution of 7-hydroxy-4-methylcoumarin. Results on rat and human plasma are in good agreement with those obtained by titration.
D000818:Animals; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D006801:Humans; D008722:Methods; D051381:Rats; D013050:Spectrometry, Fluorescence
D005230:Fatty Acids, Nonesterified