Prostaglandin production by methylcholanthrene-transformed mouse BALB/3T3: effect of oxidative phosphorylation inhibitors.
Oligomycin, antimycin, and 2,4-dinitrophenol, compounds that are known to inhibit oxidative phosphorylation by different mechanisms, inhibit the production of prostaglandins by serum-stimulated MC5-5 cells. The prostaglandin production that is stimulated by thrombin and bradykinin is inhibited by 2,4-dinitrophenol. Prostaglandin synthesis by MC5-5 cells from exogeneously-supplied arachidonic acid, however, is not affected by 2,4-dinitrophenol. Antimycin and 2,4-dinitrophenol also inhibit the serum-stimulated release of arachidonic acid from the cellular lipids, suggesting that it is the expression of phospholipase activity, a prerequisite for synthesis of prostaglandins by MC5-5 cells, that is dependent on oxidative phosphorylation.
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001095:Arachidonic Acids / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D004140:Dinitrophenols / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D008748:Methylcholanthrene; D051379:Mice; D008815:Mice, Inbred Strains; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D013917:Thrombin / Q000494:pharmacology
D001095:Arachidonic Acids; D004140:Dinitrophenols; D009840:Oligomycins; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D008748:Methylcholanthrene; D000968:Antimycin A; D013917:Thrombin; D001920:Bradykinin