High performance liquid chromatographic determination of sorbic acid in wine.
A method is described for the qualitative and quantitative determination of 2,4-hexadienoic acid (sorbic acid) by high performance liquid chromatography (HPLC). The results show excellent reproducibility (+/- 2.55%) and agree well with values obtained using the official AOAC ultraviolet method for both fortified wine and commercial wines. Sorbic acid is separated by HPLC, using a strong anion exchange resin, Zipax SAX, eluted with 0.01M Na2B4O7, and detected using UV (254 nm) detector. Sodium benzoate is used as an internal standard.
D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D013011:Sorbic Acid / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D005231:Fatty Acids, Unsaturated; D013011:Sorbic Acid