title: Leakage of sucrose from phosphatidylcholine liposomes induced by interaction with serum albumin.
abstract: Liposomes composed of rat-liver phosphatidylcholine rapidly lose entrapped sucrose when incubated in presence of blood or of solutions of bovine serum albumin. The phenomenon can not be ascribed to phospholipase A activity, since no such activity towards phosphatidylcholine substrates could be detected in various albumin preparations. Upon gel filtration on Sepharose 4B or Sephadex G-100 of incubated mixtures of radioactive liposomes and albumin, association of phosphatidylcholine with the albumin could be demonstrated. No measurable quantities of protein were found associated with liposomes. The albumin-associated phosphatidylcholine is hydrolyzed by pancreatic phospholipase A more slowly than free liposomal phosphatidylcholine, indicating a non-lamellar orientation of the associated phospholipid. The binding of phosphatidylcholine to albumin proceeds at a slow rate: increase of the amount of phosphatidylcholine bound continues over a period of several hours reaching a maximum at approx. 1 mol of phosphatidylcholine per mol of albumin. The process is reversible as indicated by transfer of albumin-associated radioactive phosphatidylcholine to unlabeled liposomes. The association between albumin and phosphatidylcholine is believed to be of the same type as described recently by Jonas (Jonas, A. (1976) Biochim. Biophy. Acta 427, 325-336). The consequences of these observations are discussed with respect to the use of liposomes as carriers to introduce substances into cells.
mesh_terms: D001769:Blood; D055598:Chemical Phenomena; D002621:Chemistry; D007444:Inulin; D008081:Liposomes; D010713:Phosphatidylcholines; D010740:Phospholipases; D012710:Serum Albumin, Bovine; D013395:Sucrose
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D012710:Serum Albumin, Bovine; D013395:Sucrose; D007444:Inulin; D010740:Phospholipases