Application of affinity electrophoresis to the study of antigen-immunoadsorbent association equilibrium.
Affinity electrophoresis is used to study the interaction of complementary biological molecules. Using Scatchard plots, this method gives a partition ratio between immunoadsorbent and soluble antigen or antibody. In the present work, affinity electrophoresis, used to study the interaction between human serum albumin and its insolubilized antibodies, allows a measure of the system's affinity under specific conditions, in particular, the electric field.
D000937:Antigen-Antibody Reactions; D005069:Evaluation Studies as Topic; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D007164:Immunosorbents; D007700:Kinetics; D008433:Mathematics; D012709:Serum Albumin / Q000276:immunology; D013223:Statistics as Topic
D007164:Immunosorbents; D012709:Serum Albumin