Preliminary X-ray crystallographic and physico-chemical investigations of human ceruloplasmin.
Single crystals of the plasma protein ceruloplasmin (CP) and its two modified forms: neuraminidase-treated CP (asialoCP) and NaN3-inhibited CP (NaN3-CP) suitable for X-ray studies have been grown. The native CP crystallizes as described previously by Magdoff-Fairchield et al. (1969) in the tetragonal space group 14 (a = b = 268.2 A, c = 129.1 A) with two protein molecules in the asymmetric part of a unit cell. AsialoCP crystals belong to the trigonal space group P 3(1)21 or P321 (a = b = 215.0 A, c = 84.5 A) and have one protein molecule in the asymmetric part of a unit cell. NaN3-CP crystals are isomorphous to crystals of native CP. Despite some differences in electrophoretic mobility and optical properties, the conformations of the native CP molecule and its modified forms are similar, as can be concluded from a study of ORD and CD spectra.
D001386:Azides / Q000494:pharmacology; D002570:Ceruloplasmin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D009439:Neuraminidase / Q000494:pharmacology; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D012794:Sialic Acids / Q000032:analysis; D014961:X-Ray Diffraction
D001386:Azides; D012794:Sialic Acids; D002570:Ceruloplasmin; D009439:Neuraminidase