Primary structure of rabbit skeletal muscle troponin-T. Sequence determination of four cyanogen bromide fragments, CB4, CB6, and CB7.
The amino acid sequences of four cyanogen bromide (CB) fragments from rabbit skeletal troponin-T have been determined. Fragment CB4 (55 residues) was documented by chymotryptic, thermolytic, tryptic, and 2-(2-nitrophenylsulfenyl)-3-methyl-3'-bromoindolenine peptides. A combination of automated sequence analysis, tryptic digestion, and penicillocarboxypeptidase-S treatment was used to assemble fragments CB5 (24 residues), CB7 (8 residues), and the COOH-terminal CB6 (21 residues).
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002268:Carboxypeptidases; D003488:Cyanogen Bromide; D009124:Muscle Proteins; D009132:Muscles; D010446:Peptide Fragments / Q000032:analysis; D011817:Rabbits; D014336:Troponin; D014357:Trypsin
D000596:Amino Acids; D009124:Muscle Proteins; D010446:Peptide Fragments; D014336:Troponin; D002268:Carboxypeptidases; C022326:penicillocarboxypeptidase-S; D014357:Trypsin; D003488:Cyanogen Bromide