title: An investigation of some problems concerning nucleolus organizers in salamanders.
abstract: Observed differences in the sizes of lampbrush nucleolus organizers in Plethodon cinereus have been shown by in situ hybridization to reflect true molecular differences in the numbers of ribosomal cistrons located at these organizers. Likewise, from in situ hybridization experiments on lampbrush and spermatocyte chromosomes it has been shown that animals may be, and indeed usually are, heterozygous with respect to the numbers of ribosomal cistrons on each half of the nucleolus bivalent. Filter hybridizations carried out on 33 males from a New Jersey population and 20 males from a Connecticut population have shown a 7.5-fold range in the numbers of ribosomal cistrons per diploid cell in the New Jersey population, and a 2.5-fold range in the Connecticut population. In view of the general heterozygosity of nucleolus organizers in these animals, the actual range in nucleolus organizer sizes in the New Jersey population is estimated to be at least 15-fold.
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002875:Chromosomes / Q000032:analysis / Q000648:ultrastructure; D004247:DNA / Q000032:analysis*; D005796:Genes; D014644:Genetic Variation; D066298:In Vitro Techniques; D008297:Male; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000032:analysis*; D014562:Caudata
chemical_list: D012335:RNA, Ribosomal; D004247:DNA