title: Prostaglandins in the isolated testicular capsule of immature and young adult rats.
abstract: The presence of prostaglandins (PGs) E1, E2, F1alpha, F2alpha and the metabolite 13,14-Dihydro-15-keto PG Falpha (1+2) has been studied in the isolated testicular capsule of Wistar rats (22 to 90 days of age). Handling and homogenization of tissues were controlled and the in vitro synthesis and degradation were prevented by immediate freezing of samples and homogenization in a solution containing a PG synthetase inhibitor. The PGs were measured by specific and sensitive radioimmunoassay. The isolated rat testicular capsule was found to contain mainly PG E2 and PG F2alpha at concentrations about 100 times higher than those in decapsulated testes (ng/g of tissue).
mesh_terms: D000367:Age Factors; D000818:Animals; D008297:Male; D009929:Organ Size; D011458:Prostaglandins E / Q000032:analysis*; D011460:Prostaglandins F / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D013737:Testis / Q000032:analysis*
chemical_list: D011458:Prostaglandins E; D011460:Prostaglandins F