title: Freeze-fracture of monolayer cultures.
abstract: This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze-fracturing.
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D005614:Freeze Fracturing / Q000379:methods*