title: [Some biochemical properties of Acholeplasma laidlawii mutants defective in glucose carrier].
abstract: Some membrane properties of glucose transport system of Acholeplasma laidlawii mutants uncapable of uptaking glucose as well as those of its non-metabolizing analogue, 3-0-methylglucose are studied. In contrast to the wild strain the mutant PTL-S cells fail to uptake carbohydrates with the saturation. There is no competition between carbohydrates during transport in this mutant no sugar exit is observed, and the sensitivity of transport to thiol reagents and pronase B is considerably affected. All other investigated membrane functions were intact. The data given indicate the presence of a specific defect in glucose transport system.
mesh_terms: D000128:Acholeplasma laidlawii / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D008757:Methylglucosides / Q000378:metabolism; D009154:Mutation; D011402:Pronase / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology
chemical_list: D008757:Methylglucosides; D013439:Sulfhydryl Reagents; D011402:Pronase; D005947:Glucose
vernacular_title: Biokhimicheskie svoistva mutantov Acholeplasma laidlaw II