[Mechanism of histone action on mitochondrial energetics. Proton and cation transport across membrane].
The effect of lysine-rich histone on mitochondria was studied with respect to the cation transport and proton uptake across the membrane. Histone does not affect Ca2+ uptake or level in mitochondria. It extrudes Mg2+ by 30% with the utilization of substrates; in the presence of rotenone extrusion drops to about 18%. Dinitrophenol and ADP prevent the effect of histone. Mg2+ uptake is inhibited by 12% by histone. K+ extrusion by histone (by 50%) which depends on the oxidation of substrates, is enhanced by phosphate, and Mg2+ decreases (by 50%) the K+ extrusion. Histone prevents to some extent extrusion of K+ by Ca2+. Histone does not affect Na+ level in mitochondria, it blocks (by 50%) Na+ uptake in the presence of gramicidin, probably by forming a gramicidin-histone complex. Histone blocks proton uptake by mitochondria incubated in the presence of valinomycin or DNP. In the presence of DNP valinomycin-induced H+ uptake is not affected by histone. H+ uptake induced by nigericin is not affected by histone as well.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism*; D002414:Cations, Monovalent / Q000378:metabolism*; D006657:Histones / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008239:Lysine; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D011188:Potassium / Q000378:metabolism; D011522:Protons; D051381:Rats; D012964:Sodium / Q000378:metabolism
D000900:Anti-Bacterial Agents; D002413:Cations, Divalent; D002414:Cations, Monovalent; D006657:Histones; D011522:Protons; D012964:Sodium; D008274:Magnesium; D008239:Lysine; D011188:Potassium; D002118:Calcium
Mekhanizm deistviia gistonov na transport protonov i kationov cherez membrany mitokhondrii