title: Glycosidases of the guinea pig brush border membrane.
abstract: The separation by polyacrylamide gel electrophoresis and subsequent enzymatic analysis of the components of the guinea pig intestinal brush border membrane revealed the presence of three enzyme complexes: maltase-glucoamylase, maltase-sucrase-glucoamylase and maltase-sucrase. Additional bands possessing lactase, trehalase and alkaline phosphatase activity were identified but no phlorizin hydrolase or palatinase was detectable. After exposure to strong dissociating conditions the bands possessing enzymatic activity were either absent or greatly reduced in intensity.
mesh_terms: D000469:Alkaline Phosphatase / Q000302:isolation & purification; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D005959:Glucosidases / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000201:enzymology*; D007421:Intestine, Small; D013393:Sucrase / Q000302:isolation & purification; D014198:Trehalase / Q000302:isolation & purification
chemical_list: D000469:Alkaline Phosphatase; D005959:Glucosidases; D006026:Glycoside Hydrolases; D014198:Trehalase; D013393:Sucrase