[Differentiation of mycobacteria by the Jones-Mote reaction (author's transl)].
The specificity of the Jones-Mote reaction to the mycobacterial species was investigated in mycobacteria-free guinea pigs. To the sensitization and the sensitine production the following type strains (Trudeau Institute Saranac Lake) were used: M. avium, M. borstelense, M.chelonei, M. flavescens, M. fortuitum, M. gastri, M. gordonae, M.kansaii, M. marinum, M nonchromogenicum, M. phlei, M. scrofulaceum, M. smegmatis, M. terrae, M. triviale and M. bovis strain Vallee as well as M. intracellulare serotyp Davis ATCC 23435. Eight days after intracutaneous sensitization with 0,2 mg of bacterial mass (wett weight) in Al(OH)3 as adjuvans the groups of 10 animals for each species were tested intracutaneously with all 18 sensitins in a concentration of 5 mug. The animals reacted positive with the homologous sensitin only; (tab. 1) a cross-reaction was stated between M. avium and M. intracellulare (closely related) and between M. borstelense and M. chelonei (the same species). The homogeneity of the Jones-Mote reaction in some rapidly growing Mycobacteria was investigated with 11 strains of M. diernhoferi, 10 strains of M. smegmatis, M. phlei, M. fortuitum and 3 strains of M. borstelense. The positive reaction was stated with species-specific sensitin only. It should be possible to identify the primocultures using mycobacteria-free guinea pigs and suitable sensitins.
D000818:Animals; D003429:Cross Reactions; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D007428:Intradermal Tests; D009161:Mycobacterium / Q000145:classification* / Q000276:immunology; D009170:Nontuberculous Mycobacteria / Q000145:classification
Differenzierung von Mykobakterien mit der Jones-Mote-Reaktion