The isolation of minimally degraded hyaluronate from rat skin.
Hyaluronate was isolated quantitatively from fresh rat skin by homogenization in an Edebo [J. Biochem. Microbiol. Technol. Eng. 2, (1960) 453-479] press, extraction with buffered saline, selective elution from DEAE-cellulose, and gel chromatography on Sephadex G-200. Traces of a protein contaminant remained.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D006025:Glycosaminoglycans / Q000032:analysis; D006820:Hyaluronic Acid / Q000302:isolation & purification*; D008297:Male; D051381:Rats; D012867:Skin / Q000032:analysis*
D006025:Glycosaminoglycans; D006820:Hyaluronic Acid