A precise semi-automated uricase method for determining uric acid in serum or urine.
1. A semi-automated uricase method is described for measuring uric acid in serum or urine directly at 292 nm at reduced cost. 2. The coefficient of variation within and between batch assays, is estimated to be 2.5% and 4.3% respectively, which is less than the estimated daily intrapersonal variation in uric acid levels. 3. Recovery of uric acid added to samples ranges from 98.5% to 101.2% with a mean of 99.6%. 4. The cost of reagents per sample determination is estimated at $0.05, and in batch analyses the mean time required per sample determination is 2-3 minutes.
D000704:Analysis of Variance; D001322:Autoanalysis; D003365:Costs and Cost Analysis; D005069:Evaluation Studies as Topic; D006801:Humans; D008722:Methods; D013053:Spectrophotometry; D013696:Temperature; D014503:Urate Oxidase; D014527:Uric Acid / Q000032:analysis* / Q000097:blood / Q000652:urine
D014527:Uric Acid; D014503:Urate Oxidase