Characterization of a DNA polymerase associated with an endogenous DNA-synthesizing complex isolated from human lymphoid cells.
We have shown that a membrane fraction prepared from isolated human lymphoid nuclei contains endogenous DNA-synthesizing activity which is sensitive to RNAase. We have isolated a DNA polymerase from this fraction and partially purified it to what we estimate as about 10 000-fold. Its chromatographic behavior, template specificity, sedimentation constant, pH optimum, and sensitivity to N-ethylmaleimide suggest that the activity resembles but is not identical to DNA polymerase gamma (formerly called R-DNA polymerase). The membrane fraction also contains a minor activity which is due to polymerase beta, the low molecular weight (3.5 S) nuclear enzyme.
D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008566:Membranes / Q000201:enzymology; D011189:Potassium Chloride / Q000494:pharmacology; D013698:Templates, Genetic
D008345:Manganese; D011189:Potassium Chloride; D004259:DNA-Directed DNA Polymerase; D008274:Magnesium