Quality control in the simultaneous assay of anticonvulsants using an automated gas chromatographic system with a nitrogen detector.
An automated gas-liquid chromatographic technique for the routine determination of phenobarbitone, primidone and phenytoin using a nitrogen-detector is described. The high selectivity of the detector for nitrogen-containing compounds allows a direct extraction procedure and the use of a phenytoin analogue as an internal standard obviates the need for accurate aliquot measurement after extraction. The anticonvulsants are chromatographed as methyl derivatives produced by on-column methylation with trimethylphenyl ammonium hydroxide following automatic liquid injection. Quality control data from an internal quality control scheme is presented and the performance of the laboratory in an inter-laboratory control scheme is reported and discussed.
D000927:Anticonvulsants / Q000097:blood*; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008745:Methylation; D009584:Nitrogen; D010634:Phenobarbital / Q000097:blood; D010672:Phenytoin / Q000097:blood; D011324:Primidone / Q000097:blood; D011786:Quality Control
D000927:Anticonvulsants; D011324:Primidone; D010672:Phenytoin; D009584:Nitrogen; D010634:Phenobarbital