title: Defluorination of methoxyflurane by a glutathione-dependent enzyme.
abstract: Partial purification from rat liver of an enzyme which catalyzes defluorination of methoxyflurane is described. Fractionation of liver homogenates by protamine sulfate and ammonium sulfate precipitation and by Sephadex G-100 chromatography results in a 10-fold purification with 53% recovery. The enzyme requires glutathione for activity, and other sulfhydrhyl compounds cannot be substituted. The enzyme appears to be a glutathione S-transferase, possibly one of several which have recently been characterized.
mesh_terms: D000818:Animals; D005461:Fluorine / Q000378:metabolism; D005978:Glutathione; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008733:Methoxyflurane / Q000378:metabolism*; D051381:Rats; D013438:Sulfhydryl Compounds
chemical_list: D013438:Sulfhydryl Compounds; D005461:Fluorine; D008733:Methoxyflurane; D005978:Glutathione