Effect of age, sex, and intestinal flora on the induction of colon tumors in rats.
Germfree and conventional Sprague-Dawley rats were assessed for their susceptibility to intrarectally injected N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or N-methyl-N-nitrosourea (MNU). Adenocarcinoma of the colon was induced in germfree and conventional rats by both MNNG and MNU. The colons of germfree rats were more susceptible to the direct-acting carcinogens, as manifested by earlier morbidity and development of colon tumors (50% tumors within 30-35 wk), than were those of conventional rats (50% colon tumors within 48-50 wk). Germfree and conventional male rats were more susceptible to the carconogens than were their female germfree and conventional counterparts. Young (30 days old at the start of the experiment) germfree rats developed colon tumors more quickly (15-20 wk) than did older (60 days) germfree rats after intrarectal injections of MNNG. No colon tumors were observed in germfree and conventional rats after 75 weekly intrarectal injections with a buffer. Transplantation of an adenocarcinoma induced with MNU in a female rat to germfree and conventional rats showed that it was easily transplantable, required no immunosuppression, and had essentially the same morphologic characteristics as did the primary tumor.
D000230:Adenocarcinoma / Q000139:chemically induced / Q000209:etiology* / Q000473:pathology; D000367:Age Factors; D000818:Animals; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000209:etiology* / Q000473:pathology; D004334:Drug Administration Schedule; D005260:Female; D005856:Germ-Free Life; D007422:Intestines / Q000382:microbiology*; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000008:administration & dosage; D008770:Methylnitrosourea / Q000008:administration & dosage; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000209:etiology / Q000473:pathology; D051381:Rats; D012737:Sex Factors; D013997:Time Factors; D014184:Transplantation, Homologous
D008769:Methylnitronitrosoguanidine; D008770:Methylnitrosourea