Preparation and high-performance liquid chromatography of iodinated diethylstilbestrols and some related steroids.
Diethylstilbestrol and estradiol have been converted to their mono- and poly-iodinated derivatives by electrophilic substitution of positive iodine into the activated aromatic rings. Both chemical and electrochemical procedures have been employed for the iodination, and the latter procedure proved to be superior. These iodinated derivatives have been purified by preparative high-performance liquid chromatography, and characterized by ultraviolet and mass spectroscopy.
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004054:Diethylstilbestrol / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis; D004563:Electrochemistry; D004958:Estradiol / Q000031:analogs & derivatives / Q000138:chemical synthesis; D007455:Iodine; D013058:Mass Spectrometry; D008722:Methods; D013056:Spectrophotometry, Ultraviolet
D004958:Estradiol; D004054:Diethylstilbestrol; D007455:Iodine