title: Transfection of Streptococcus sanguis by phage deoxyribonucleic acid isolated from Streptococcus mutans.
abstract: Streptococcus sanguis ATCC 10556 cells were infected with free phage DNA of S, mutans strain PK 1. Two transformants were isolated which made colonies with large mucoid forms on mitis-salivarius agar. Both transformants had an increased ability to synthesize insoluble glucan and showed an adhesive nature on glass surfaces. These characteristics of the transformants bear a resemblance to S. mutans. These transformants had many physiological characteristics by which they could be recognized as S. sanguis. However, they resembled S. salivarius in forming a large amount of soluble fructan. Furthermore, the transformant cells did not produce ammonia from arginine, whereas their parent cells did.
mesh_terms: D001433:Bacteriolysis / Q000187:drug effects; D001435:Bacteriophages; D002448:Cell Adhesion; D004279:DNA, Viral; D005898:Glass; D005998:Glycine / Q000096:biosynthesis; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D013291:Streptococcus; D013295:Streptococcus mutans; D013298:Streptococcus sanguis; D014170:Transformation, Genetic
chemical_list: D004279:DNA, Viral; D008937:Mitomycins; D011135:Polysaccharides, Bacterial; D005998:Glycine