Rapid sensitive fluorometric analysis of cephalosporin antibiotics.
A rapid and sensitive fluorometric analysis for cephalosporins, which can also be applied to penicillins, is presented. The method involves reaction with 0.1 N sodium hydroxide at 100 degrees, producing stable fluorescent products. This method was applied to cephalexin and ampicillin with detection at concentrations as low as 0.01 mug/ml.
D000667:Ampicillin / Q000097:blood; D002506:Cephalexin / Q000097:blood; D002511:Cephalosporins / Q000032:analysis*; D002512:Cephalothin / Q000032:analysis; D006358:Hot Temperature; D008722:Methods; D010406:Penicillins / Q000032:analysis; D012972:Sodium Hydroxide; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D002511:Cephalosporins; D010406:Penicillins; D012972:Sodium Hydroxide; D000667:Ampicillin; D002506:Cephalexin; D002512:Cephalothin