Effect of p-bromophenacyl bromide, an inhibitor of phospholipase A2, on arachidonic acid release and prostaglandin synthesis by the guinea-pig uterus in vitro.
The synthesis of prostaglandins F2alpha and E2 by guinea-pig uterine homogenates was inhibited by p-bromophenacyl bromide (PBPAB), an inhibitor of phospholipase A2. 2 Metabolism of prostaglandin F2alpha by uterine homogenates was undetectable; this was not affected by PBPAB. 3 There was no significant difference between the amounts of arachidonic acid released from uterine homogenates on days 7 and 1k of the oestrous cycle. Small amounts of dihomo-gamma-linolenic acid were detected in the homogenates. 4 The release of arachidonic acid from uterine homogenates was greatly inhibited by PBPAB. 5 Addition of exogenous arachidonic acid to uterine homogenates did not overcome the inhibition of uterine prostaglandin F2alpha synthesis produced by PBPAB. 6 It is concluded that PBPAB inhibits both the release of arachidonic acid from the guinea-pig uterus and its subsequent conversion into prostaglandins.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001095:Arachidonic Acids / Q000378:metabolism*; D003429:Cross Reactions; D005260:Female; D006168:Guinea Pigs; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D011458:Prostaglandins E / Q000096:biosynthesis* / Q000276:immunology; D011460:Prostaglandins F / Q000096:biosynthesis* / Q000276:immunology; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D001095:Arachidonic Acids; D011458:Prostaglandins E; D011460:Prostaglandins F; D010740:Phospholipases