A radioimmunological method for oestrone. Plasma levels of non-conjugated oestrone during uncomplicated pregnancy.
A specific antiserum to oestrone has been used to develop a radioimmunoassay. No chromatographic separation step was included. A small amount of plasma (0.01--0.15 ml) was extracted once with diethyl ehter. The method was used to estimate plasma levels of non-conjugated oestrone in women during uncomplicated late pregnancy. The oestrone plasma concentrations showed a gradual increase from a mean of 4 ng/ml in the 22nd week to 11 ng/ml in the 41st week. Great individual variations were found. No systematic changes of unconjugated oestrone plasma concentrations were noticed during a 24-hour period in late pregnancy. Short term studies revealed that large and rapid fluctuations may appear in the levels of unconjugated oestrone during pregnancy.
D004970:Estrone / Q000097:blood*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007106:Immune Sera; D007202:Indicators and Reagents; D010507:Periodicity; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D011863:Radioimmunoassay / Q000379:methods*
D007106:Immune Sera; D007202:Indicators and Reagents; D004970:Estrone