A new colorimetric method for the determination of plasma lecithin-cholesterol acyltransferase activity.
A new method has been developed to improve the linearity of the plasma lecithin-cholesterol acyltransferase reaction by adding synthetic dipalmitoyl lecithin sol to the incubation medium and subsequently measuring the change in free cholesterol content after incubation by a combined enzymatic method using cholesterol oxidase and peroxidase. The method was found to be simpler than other techniques such as gas-liquid chromatography or isotope methods and therefore may be suitable for the routine assays of plasma lecithin-cholesterol acyltransferase activities in clinical laboratories.
D000217:Acyltransferases / Q000097:blood*; D000293:Adolescent; D000328:Adult; D003124:Colorimetry / Q000379:methods; D003920:Diabetes Mellitus / Q000201:enzymology; D005069:Evaluation Studies as Topic; D006801:Humans; D007700:Kinetics; D008103:Liver Cirrhosis / Q000201:enzymology; D008875:Middle Aged; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood*
D000217:Acyltransferases; D007862:Phosphatidylcholine-Sterol O-Acyltransferase