Inversion of the unnatural cis C/D sterol ring junction of 5alpha, 14beta-cholest-7en-3beta-ol by rat-liver enzymes.
Labelled 5alpha, 14beta-cholest-7-en-3beta-ol with the 'unnatural' cis C/D ring junction was synthesized. Incubations of this sterol with rat liver homogenate under aerobic conditions gave radioactive 5alpha-cholest-7-en-3beta-ol, 5alpha-cholest-8(14)-en-3beta-ol andcholesterol indicating the presence of enzymes in the rat liver capable of inverting the C/D cis to a trans configuration. No radioactivity was found associated with added 14beta-cholesterol showing the specificity of the 14alpha configuration for the enzymic conversion of a sterol molecule into cholesterol.
D000332:Aerobiosis; D000818:Animals; D002782:Cholestenes / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D007535:Isomerases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats; D013261:Sterols / Q000378:metabolism*
D002782:Cholestenes; D013261:Sterols; D007535:Isomerases