title: The synthesis of several 8-substituted derivatives of adenosine 5'-monophosphate to study the effect of the nature of the spacer arm in affinity chromatography.
abstract: Reaction of adenosine 5'-monophosphate (Ado-5'-P) with bromine at pH 4.0 yielded 8-bromo-adenosine 5'-monophosphate and following reaction with several alpha,omega-diaminoalkanes gave the corresponding 8-(omega-aminoalkyl)-Ado-5'-P derivatives. Condensation of these analogues with N-trifluoroacetyl-glycine or beta-alanine in the presence of a water-soluble carbodiimide generated several 8-substituted derivatives. These analogues: (see article) comprised an 8-substituted Ado-5'-P ligand to which a spacer molecule of similar length but differing hydrophobicity was attached. The derivatives were purified, characterised and attached to CNBr-activated Sepharose. The chromatographic behaviour of the resulting absorbents was investigated in terms of their ability to bind both lactate and alanine dehydrogenase. The enzymes bound tighter to the more hydrophobic derivatives with the strength of the interaction decreasing with increasing hydropholicity. The effect of the introduction of a single hydroxyl group in the spacer arm was also studied with several enzymes known to exhibit anomalous behavior on affinity chromatography. In free solution, (Ado-5'-P)-8-NH CH2CH2CH2CH2CH2CH2NH2 and (Ado-5'-P)-8-NH CH2-CH CH2NH CO CH2NH2 were competitive with NADH for rabbit muscle lactate dehydrogenase, (see article) with Ki values of 2.0 and 1.8 mM respectively. These data suggest a difference in accessibility of the hydrophobic and hydrophilic derivatives when attached to Sepharose rather than a fundamental difference in affinity. This suggestion is supported by the fact that there is no obvious correlation between the Ki values in free solution of a series of 8-(omega-aminoalkyl)-Ado-5'-P derivatives of increasing chain length, and hence hydrophobicity, and their chromatographic behaviour when immobilised. The data suggest that the hydrophobicity/hydrophilicity of the spacer arm determined the accessibility of the immobilised ligand to interaction with the complementary enzyme.
mesh_terms: D000249:Adenosine Monophosphate / Q000031:analogs & derivatives*; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D004798:Enzymes / Q000302:isolation & purification*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification; D009132:Muscles / Q000201:enzymology; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
chemical_list: D004798:Enzymes; D000249:Adenosine Monophosphate; D007770:L-Lactate Dehydrogenase