Pentose utilizing variants of Novikoff hepatoma cells: modification of growth and morphological properties.
A series of variant lines that utilize multiple pentoses for growth in place of glucose have been isolated from an 8-azaguanine resistant line of Novikoff hepatoma cells (N1S167). These variants utilize for growth ribose, xylose, arabinose, and/or deoxyribose. The variants growing on pentose containing medium (a) exhibit a density dependent cessation of growth, (b) have a morphology change to a more flattened cell type, (c) become binucleated in the presence of cyto chalasin B, and (d) show an altered sensitivity to trypsin treatment.
D001089:Arabinose / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured / Q000166:cytology; D003260:Contact Inhibition; D003470:Culture Media; D003571:Cytochalasin B / Q000494:pharmacology; D003855:Deoxyribose / Q000378:metabolism; D014644:Genetic Variation; D010429:Pentoses / Q000378:metabolism*; D012266:Ribose / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology; D014994:Xylose / Q000378:metabolism
D003470:Culture Media; D010429:Pentoses; D003571:Cytochalasin B; D003855:Deoxyribose; D012266:Ribose; D014994:Xylose; D001089:Arabinose; D014357:Trypsin