title: Alteration of cell-surface antigenicity of the mouse plasmacytoma. II. Lack of correlation between synthesis of myeloma protein and alteration of surface antigen.
abstract: The correlation between the changes of immunoglobulin synthesis and the surface antigenicity was analyzed by cytotoxicity and quantitative antibody-absorption tests with the cells of immunoglobulin-producing and nonproducing mouse plasmacytoma. IgA-synthesizing BLAB/c plasmacytoma 58-8 and the non-IgA-synthesizing variant of the 58-8 (nonproducer) were killed with rabbit anti-58-8 plasmacytoma cell antiserum, C3H/He anti-BALB/c spleen cell antiserum, and (C57BL/6 X DBA/2)F1 anti-BALB/c plasmacytoma cell MOPC-31C antiserum plus complement, only when the cells were pretreated with pronase. Quantitative absorption tests revealed that the nonproducer, 58-8 had the same amount of plasmacytoma antigen of 58-8 and PC.1 antigen, and a greater amount of H-2d antigen, as did producer 58-8. The same analysis was carried out for the C3H mouse plasmacytoma X5563, which has an M-component of IgG2a. The nonproducer X5563 had a greater amount of H-2k antigen and a smaller amount of the plasmacytoma antigen of X5563 than did the producer X5563. No detectable PC.1 antigen was observed at surfaces of the producer and the nonproducer X5563 cells.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm; D002462:Cell Membrane / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D006649:Histocompatibility Antigens; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009194:Myeloma Proteins / Q000096:biosynthesis*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology; D010954:Plasmacytoma / Q000276:immunology*; D014185:Transplantation, Isogeneic
chemical_list: D000951:Antigens, Neoplasm; D006649:Histocompatibility Antigens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D009194:Myeloma Proteins