Stimulation by prostaglandin F2alpha of acidic glycosaminoglycan production in cultured fibroblasts.
The effect of PGF2alpha on the synthesis of hexosamine-containing substances (acidic glycosaminoglycans and glycoproteins) was studied in cultured fibroblasts derived from a rat carrageenin granuloma. Treatment with PGF2alpha ranging from 0.01 mug/ml to 20 mug/ml resulted in a significant increase of the production of these macromolecules by the cells. The stimulatory effect was found significant even at the low concentration of 10 ng/ml, and could be seen as early as 3h after exposure to PGF2alpha. The hexosamine-containing substances increased by PGF2alpha revealed that 80% of the increase was due to acidic glycosaminoglycans and the rest was due to glycoproteins.
D000818:Animals; D002351:Carrageenan; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006099:Granuloma / Q000139:chemically induced / Q000378:metabolism*; D006595:Hexosamines / Q000378:metabolism; D011460:Prostaglandins F / Q000494:pharmacology*; D051381:Rats; D013268:Stimulation, Chemical
D003470:Culture Media; D006023:Glycoproteins; D006025:Glycosaminoglycans; D006595:Hexosamines; D011460:Prostaglandins F; D002351:Carrageenan; D005944:Glucosamine