The effects of sex-steroids on the synthesis of uterine non-histone proteins.
Newly synthesized non-histone proteins (NHP) labelled with [35S] methionine were isolated from uterine epithelium and stroma after different hormone treatments and examined by isoelectric focusing in polyacrylamide gels. Progesterone increased the proportion of basic NHP in the epithelium. This effect was detectable within 4 h and was maintained unchanged after oestrogen stimulation, which increased the synthesis of all classes of NHP. The proportion of basic NHP was higher in the untreated stroma than in the epithelium. Progesterone pretreatment did not increase the proportion of basic NHP in the stroma but prevented the increase in the more acidic NHP which followed treatment with oestrogen alone. The tendency to synthesize a higher proportion of basic NHP did not in any way correlate with the tissue's proliferative status.
D000818:Animals; D002369:Castration; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D051379:Mice; D011374:Progesterone / Q000494:pharmacology*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; D011374:Progesterone; D004958:Estradiol