The fully automatic ion-exchange and gel-permeation chromatography of neutral monosaccharides and oligosaccharides with a Jeolco JLc-6AH analyser.
Experiences with a Jeolco JLC-6AH carbohydrate analyser used to provide a fully automated analysis service for neutral mono- and oligo-saccharides have been summarised. Modifications have been made to the equipment to extend its application, and a method for routine separations of oligosaccharides (d.p. 2--15) has been established. The degree of separation achieved is far superior to that which may be obtained by gel-filtration techniques under gravity- or peristaltic-pump-aided flow. The versatility and adaptability of equipment for use with assays other than the standard orcinol-sulphuric acid reaction shows that it is not too inconvenient to interchange between various assays and separations.
D001322:Autoanalysis; D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods; D008970:Molecular Weight; D009005:Monosaccharides / Q000302:isolation & purification*; D009844:Oligosaccharides / Q000302:isolation & purification*
D009005:Monosaccharides; D009844:Oligosaccharides