Trace analysis of zearalenone and/or zearalanol in animal chow by high pressure liquid chromatography and gas-liquid chromatography.
An analytical method is described for determining residues of the estrogens zearalenone and/or zearalanol in animal chow at levels as low as 10 ppb. The chow is extracted with methanol and cleaned up by a 3-step procedure employing a Sephadex LH-20 column, liquid-liquid partitioning at pH 13 and 8.3, and a silica gel column. Residues of the 2 compounds, separated on silica gel, are assayed by using high pressure liquid chromatography with ultraviolet detection. Additional data are also included concerning p-values of the compounds in several solvent systems, Rf values from thin layer chromatography with 9 solvent systems, solubilities in 3 solvents, and a procedure for preparing their pentafluoropropionyl derivatives for analysis by electron capture gas-liquid chromatography.
D000821:Animal Feed / Q000032:analysis*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D012118:Resorcinols / Q000032:analysis*; D015025:Zearalenone / Q000032:analysis*; D015029:Zeranol / Q000032:analysis*
D012118:Resorcinols; D015025:Zearalenone; D015029:Zeranol