Description of a new hamster ventral prostate cell line containing androgen receptors.
Using chemical transformation of primary monolayers of hamster ventral prostate tissue, we have established the HVP-G3 and HVP-B1 cloned cell lines which have been growing in our laboratories for over 18 months. Both cell lines contain high-affinity, saturable androgen receptors which migrate into the nucleus when exposed to physiological doses of androgen. However, neither cell line demonstrates a growth response to androgens. Each cell line will produce a tumor in 6 to 8 weeks when injected subcutaneously into male or female Syrian hamsters. In vitro cell doubling times range from 12 hr in the presence of luteinizing hormone (LH-NIH-S-18) to 18 hr in the presence of 5% serum. Both cell lines have a diploid stemline.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D006224:Cricetinae; D003470:Culture Media; D005260:Female; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D008647:Mesocricetus; D008748:Methylcholanthrene; D009368:Neoplasm Transplantation; D011467:Prostate / Q000166:cytology*; D011944:Receptors, Androgen; D011987:Receptors, Steroid
D003470:Culture Media; D011944:Receptors, Androgen; D011987:Receptors, Steroid; D008748:Methylcholanthrene; D007986:Luteinizing Hormone