Dialysis of gallbladder bile. Correlation between rates of exit of bile salts and bilirubin from the dialysis sac.
Twenty-six gallbladder bile samples were dialyzed against an infinite dilution of Tris buffer at pH 7.4. Bilirubin moved out of the dialysis sac at a rate slower than that of bile salts. The rates of exit of these two molecules showed a significantly positive correlation with each other and a significantly negative correlation with bile salts concentration. Phospholipids and cholesterol did not move out of the dialysis sac. These data are consistent with the assumption that bile is a mixed micellar solution of bile salts, phospholipids, and cholesterol and suggest an association between bilirubin and the mixed micelle.
D001646:Bile / Q000032:analysis*; D001647:Bile Acids and Salts / Q000032:analysis*; D001663:Bilirubin / Q000032:analysis*; D002784:Cholesterol / Q000032:analysis; D003956:Dialysis; D005704:Gallbladder; D006801:Humans; D008823:Micelles; D010743:Phospholipids / Q000032:analysis
D001647:Bile Acids and Salts; D008823:Micelles; D010743:Phospholipids; D002784:Cholesterol; D001663:Bilirubin