Mechanism for the incorporation of S-(1,2,3,4-tetrahydro-2-hydroxy-1-naphthyl)-L-cysteine into protein.
Evidence is presented which indicates that S-(1,2,3,4-tetrahydro-2-hydroxy-1-naphthyl)-L-cysteine (THN-cysteine), formed by the reaction of 1,2-epoxy-THN with cysteine, can be incorporated into protein; The position of incorporation of THN-cysteine into protein would depend on whether the epoxide of THN reacts with cysteinyl-tRNACyS or with cysteine. In both cases, the mechanism of incorporation of THN-cysteine into protein is the same as for the natural amino acids. For example, the incorporation of THN-cysteinyl-tRNACyS is stimulated by Poly-UG, the code for tRNACyS, and would be expected to be substituted for cysteine in protein being synthesized, whereas THN-cysteine not previously esterified to tRNA is activated by the isoleucyl- and valyl-RNA synthetases, and its incorporation is stimulated by Poly-AU and Poly-UG, respectively. Consequently, in this case, THN-cysteine would substitute for isoleucine and valine during protein synthesis.
D003545:Cysteine / Q000378:metabolism; D007532:Isoleucine / Q000378:metabolism; D011119:Polynucleotides / Q000494:pharmacology; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism; D012269:Ribosomal Proteins / Q000096:biosynthesis; D014633:Valine / Q000378:metabolism
D011119:Polynucleotides; D012269:Ribosomal Proteins; D007532:Isoleucine; D012343:RNA, Transfer; D014633:Valine; D003545:Cysteine