Thin layer chromatography and fluorescent scanning analysis of porphyrins.
A combined technique of two dimensional thin layer chromatography with fluorescent scanning was applied to the estimation of urinary porphyrins in 2 patients with porphyria cutanea tarda. Porphyrin patterns were also examined with the technique on urine, liver and skin samples obtained from a hexachlorobenzene-induced porphyric rat. Results obtained showed that uroporphyrin and hepta-carboxylic porphyrin were predominant in the urine, liver and skin.
D000328:Adult; D000818:Animals; D002855:Chromatography, Thin Layer; D005470:Fluorometry; D006801:Humans; D008099:Liver / Q000032:analysis; D008297:Male; D008875:Middle Aged; D011164:Porphyrias / Q000652:urine*; D011166:Porphyrins / Q000032:analysis / Q000652:urine*; D051381:Rats; D012867:Skin / Q000032:analysis; D014578:Uroporphyrins / Q000652:urine
D011166:Porphyrins; D014578:Uroporphyrins