Platelet activating factor, the trigger of haemostatic alterations in rat anaphylaxis.
Platelet-activating factor (PAF) generated by an IgE-mediated reaction in the peritoneal cabity of rats was partially purified by adsorption to diatomaceous earth. It aggregated rat platelets and, as a consequence, activated Hageman factor in in vitro, as well as in vivo, conditions. The haemostatic alterations induced by PAF showed similarity to those observed in the early phase of rat anaphylaxis.
D000707:Anaphylaxis / Q000276:immunology*; D000818:Animals; D001491:Basophils; D001777:Blood Coagulation; D001779:Blood Coagulation Factors; D005174:Factor XII; D006487:Hemostasis; D010974:Platelet Aggregation; D051381:Rats
D001779:Blood Coagulation Factors; D005174:Factor XII