[The influence of extracellular calcium on volume, enzyme and calcium secretion in the isolated perfused canine pancreas (author's transl)].
1. The kinetics of Ca++ and enzyme secretion are of corresponding pattern as well at hydrokinetic as at ecbolic stimulation. 2. Physiological respectively pathophysiologically relevant changes of perfusate Ca++ concentrations do not influence pancreatic Ca++ secretion. Extracellular Ca++ concentrations beyond 12 mEq/1 initiate an enzyme independent Ca++ secretion. 3. Hydrokinetic or ecbolic stimulated pancreatic secretion do not distinguish in calcium/protein ratio. 4. Basal and stimulated enzyme secretion of isolated perfused canine pancreas remain unaffected by increments of extracellular Ca++ concentrations even at pharmacological values. 5. The stimulated enzyme- and Ca++ secretion remain constant during hypocalcemic perfusate conditions but decrease to basal values in Ca++ free media, rapidly and completely reversible by recalcification of the perfusate. 6. The secretin stimulated volume secretion remains unchanged either by extremely hypercalcemic or Ca++ depleted perfusate conditions.
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002766:Cholecystokinin / Q000494:pharmacology; D004285:Dogs; D004798:Enzymes / Q000378:metabolism; D006934:Hypercalcemia / Q000378:metabolism; D066298:In Vitro Techniques; D010179:Pancreas / Q000378:metabolism*; D010189:Pancreatic Juice / Q000378:metabolism; D010477:Perfusion; D012633:Secretin / Q000494:pharmacology
D004798:Enzymes; D012633:Secretin; D002766:Cholecystokinin; D002118:Calcium
Der Einfluss des extrazellulären Calciums auf die Saft-, Enzym- und Calciumsekretion des isoliert perfundierten Hundepankreas