title: Cell wall carbohydrates in Phycomyces blakeslesanus Burgeff.
abstract: The carbohydrate composition of the cell walls from spores, mycelium and sporangiophores of Phycomyces blakesleeanus was analyzed. Spore wall polysaccharides contained over 50% glucose, about 20% uronic acids, 10% mannose and 10% amino-sugars. During the growth of the hyphae amino-sugars became the main carbohydrate (45%); uronic acids contributed some 25%, glucose and fructose 10% and galactose nearly 6%. Sporangiophores contained almost 90% aminosurgars and some 6% uronic acids. Traces of rhamnose were found in all wall preparations. A similar picture emerged from studies on the incorporation of [U-14C]-glucose into wall materials. Furthermore we looked for a GDP-fucose synthesizing system and found an increasing activity during early germination. This rise in activity was inhibited by cycloheximide but only by 5-fluorouracil.
mesh_terms: D000606:Amino Sugars / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D005658:Fungi / Q000032:analysis*; D006154:Guanosine Diphosphate Fucose / Q000096:biosynthesis; D006601:Hexoses / Q000032:analysis; D010800:Phycomyces / Q000032:analysis* / Q000502:physiology; D011134:Polysaccharides / Q000032:analysis*; D013172:Spores, Fungal / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis
chemical_list: D000606:Amino Sugars; D006601:Hexoses; D011134:Polysaccharides; D014574:Uronic Acids; D006154:Guanosine Diphosphate Fucose