The trypsin and chymotrypsin inhibitors in chick pears (Cicer arietinum L). The relationships among the six isoinhibitors.
The six isoinhibitors of trypsin and chymotrypsin which were previously purified from a crude extract of chick peas were further studied with the intention of establishing the relationships among them.  Exposure of each fraction to active, matrix-bound trypsin resulted in a decrease of the specific inhibitory activities towards trypsin of fractions P-1, P-3 and P-5 while those of P-4 and P-6 were increased. Each of these fractions was separated into several sub-fractions after chromatography on a DEAE-Sephadex A-25 column under conditions identical to those used previously for their purification. The elution positions, specific inhibitory activities towards trypsin and electrophoretic patterns in polyacrylamide gels of the various sub-fractions were determined...
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D010940:Plant Proteins / Q000302:isolation & purification; D012639:Seeds / Q000032:analysis*; D014361:Trypsin Inhibitors / Q000302:isolation & purification
D000596:Amino Acids; D010446:Peptide Fragments; D010940:Plant Proteins; D014361:Trypsin Inhibitors; D002918:Chymotrypsin