[Effect of N-ethylenemaleimide on the inhibitory activity of cytostatics].
The cytostatic effect of endoxan, Tiotef, bleomycin and other drugs was increased by addition of a thioloprovic compound of N-ethylenmaleimide (N-EM) to the cultures of cancer cells in doses not affecting the synthesis of nucleic acids in these cultures. The sensibilizing effect of N-EM against the antitumor drugs was due to blocking of SH-enzymes responsible of synthesis or reparation of DNA. Thus, a simultaneous effect of N-EM on the thiol cycle and synthesis of nucleic acids was synergistic for a number of antitumor drugs. It provided an increased effect of the cytostatics in doses having no activity under conditions of routine in vitro tests.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000037:antagonists & inhibitors; D003864:Depression, Chemical; D004357:Drug Synergism; D005033:Ethylmaleimide / Q000494:pharmacology*; D066298:In Vitro Techniques; D007928:Lethal Dose 50; D007942:Leukemia, Experimental / Q000188:drug therapy; D051379:Mice; D012334:RNA, Neoplasm / Q000037:antagonists & inhibitors; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D000903:Antibiotics, Antineoplastic; D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D013439:Sulfhydryl Reagents; D005033:Ethylmaleimide
Vliianie N-étilenmaleimida na ingibiruiushchuiu aktivnost'tsitostatikov