Mitochondrial biogenesis in cultured mammalian cells. III. Synthesis of mitochondrial phospholipids by subcellular fractions isolated from normal and chloramphenicol-treated BHK-21 cells.
The capacity of subcellular fractions isolated from chloramphenicol-treated BHK-21 cells to synthesize various mitochondrial phospholipids in vitro and examined. Both mitochondria and microsomes showed the capacity to acylate sn-glycerol 3-phosphate and dihydroxyacetone phosphate to lysophosphatidic acid and acyldihydroxyacetone phosphate and subsequently to phosphatidic acid. Both processes are inhibited in mitochondria from chloramphenicol-treated cells. The synthesis of CDPdiacylglycerol in mitochondria or microsomes, and the synthesis of phosphatidylinositol and of phosphatidylcholine in microsomes were stimulated in treated cells. A slight stimulation was also observed in the synthesis of phosphatidylglycerol and diphosphatidylglycerol when the labelled precursor was sn-glycerol 3-phosphate in treated cells, although the process was inhibited with labelled glycerol as the precursor. Conversion of phosphatidylglycerol phosphate to phosphatidylglycerol by mitochondria was rate limiting unless the post-microsomal supernatant fraction was added. These results are discussed in regard to the observed inhibition of phospholipid synthesis in BHK-21 cells in culture by chloramphenicol.
D002460:Cell Line; D002701:Chloramphenicol / Q000494:pharmacology*; D003035:Cobalt / Q000494:pharmacology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
D010743:Phospholipids; D003035:Cobalt; D002701:Chloramphenicol; D008274:Magnesium