[Sensitivity of the lymphocyte RNA-synthesizing system of patients with different malignant neoplasms to phytohemagglutinin and dexamethasone].
RNA biosynthesis catalyzed with DNA-dependent RNA polymerase was demonstrated in the reconstructed system containing isolated lymphocyte nuclei, Mg2+ or Mn2+ salts, ammonium sulphate, in the presence of four nucleosidetriphosphates. Both the Mg2+ and Mn2+-dependent forms of this enzyme were revealed in the nuclei of normal lymphocytes and those of patients suffering from melanoma, carcinoma of the lung and sarcoma. The activities of both forms of RNA-polymerase were greater in the nuclei of the lymphocytes from sick individuals than in the normal analogues. DNA-dependent RNA-polymerase sensitivity to dexamethasone and PHA of the nuclei of lymphocytes obtained from patients with carcinoma of the lung, melanoma, and sarcoma was decreased in comparison with the normal.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008175:Lung Neoplasms / Q000097:blood / Q000201:enzymology*; D008214:Lymphocytes / Q000201:enzymology*; D008274:Magnesium; D008345:Manganese; D008545:Melanoma / Q000097:blood / Q000201:enzymology*; D012509:Sarcoma / Q000097:blood / Q000201:enzymology*
D037102:Lectins; D008345:Manganese; D003907:Dexamethasone; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium
Chuvstvitel'nost' RNK-sinteziruiushcheĭ sistemy limfotsitov bol'nykh s razlichnymi zlokachestvennymi novoobrazovaniiami k fitogemaggliutininu i deksametazonu