Immunogenicity of p-phenetidine, 2-hydroxy-p-phenetidine and their protein conjugates in guinea pigs, rabbits and man.
Guinea pigs were sensitized by p-phenetidine (PT), 2-hydroxy-p-phenetidine (HPT) as well as by conjugates prepared by reacting PT and HPT with proteins in vitro. Sensitization was evaluated by delayed skin reactivity and in vitro antigen-induced lymphocyte proleferation. HPT and HPT-protein conjugates were found to be the most effective sensitizing agents. Reaginic antibodies could be raised in both guinea pigs and rabbits by immunizing with PT- and HPT-protein conjugates but not by PT and HPT alone: these PCA antibodies showed strong cross-reactivity and could be elicited equally well with either  the PT- or HPT-protein derivatives. By contrast, no precipitating antibodies could be raised in either species even after repeated immunizations over a period of 4 months. Peripheral blood lymphocytes, from a few patients who gave a positive patch test with PT, could be stiumlated in vitro with phenacetin and to a lesser degree with PT and with a HPT-derivative of human serum albumin.
D000814:Aniline Compounds / Q000276:immunology*; D000818:Animals; D000941:Antigens; D003429:Cross Reactions; D006168:Guinea Pigs; D006801:Humans; D006968:Hypersensitivity, Delayed; D007073:Immunoglobulin E / Q000032:analysis; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D010628:Phenetidine / Q000031:analogs & derivatives / Q000276:immunology*; D012709:Serum Albumin / Q000276:immunology
D000814:Aniline Compounds; D000941:Antigens; D012709:Serum Albumin; D010628:Phenetidine; D007073:Immunoglobulin E