title: The proteins in the Z line of insect flight muscle.
abstract: Z discs were isolated from Lethocerus flight muscle by removing the contractile proteins from myofibrils with a solution of high ionic strength. The protein composition of the Z discs was analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis; the major proteins were alpha-actinin, actin and tropomyosin. Z lines were selectively removed from intact myofibrils by digestion with crude lipase and chymotrypsin, but not by purified lipase.
mesh_terms: D000818:Animals; D002918:Chymotrypsin; D004591:Electrophoresis, Polyacrylamide Gel; D006430:Hemiptera / Q000032:analysis*; D008049:Lipase; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000032:analysis* / Q000648:ultrastructure; D009210:Myofibrils / Q000032:analysis
chemical_list: D009124:Muscle Proteins; D008049:Lipase; D002918:Chymotrypsin