[Effect of urethane (DAB-6 product), hexobarbital-Na and pentobarbital-Na on the amino acid concentrations in blood serum of rats. I. Communication (author's transl)].
Free amino acids were determined quantitatively in serum from the fasting blood of rats before and after treatment with urethane (n = 20), hexobarbital-Na (n = 20) and pentobarbital-Na (n = 20). Serum was deproteinised by adding an equal volume of 5% sulphosalicyclic acid and the amino acids were separated by ion exchange column chromatography.


RESULTS
1.24 h after treatment with urethane the rats showed only a statistically significant higher concentration of ornithine in serum. - 2.24 h after injection of hexobarbital-Na the rats had statistically significant higher concentrations of arginine, isoleucine, leucine, lysine, methionine, phenylalanine, taurine, tryptophan and tyrosine in serum - 3.24 h after application of pentobarbital-Na the rats showed statistically significant higher concentrations of aspartic acid, glutamic acid, histidine, lysine, phenylalanine, serine, taurine, threonine, and tyrosine and statistically significant lower concentrations of argine and citrulline in serum.
D000596:Amino Acids / Q000097:blood*; D000818:Animals; D001120:Arginine / Q000097:blood; D002852:Chromatography, Ion Exchange; D006591:Hexobarbital / Q000494:pharmacology*; D007532:Isoleucine / Q000097:blood; D007930:Leucine / Q000097:blood; D008239:Lysine / Q000097:blood; D008297:Male; D008715:Methionine / Q000097:blood; D008722:Methods; D009952:Ornithine / Q000097:blood; D010424:Pentobarbital / Q000494:pharmacology*; D010649:Phenylalanine / Q000097:blood; D051381:Rats; D013654:Taurine / Q000097:blood; D014364:Tryptophan / Q000097:blood; D014443:Tyrosine / Q000097:blood; D014520:Urethane / Q000494:pharmacology*
D000596:Amino Acids; D007532:Isoleucine; D013654:Taurine; D014520:Urethane; D014443:Tyrosine; D010649:Phenylalanine; D014364:Tryptophan; D001120:Arginine; D008715:Methionine; D006591:Hexobarbital; D009952:Ornithine; D007930:Leucine; D010424:Pentobarbital; D008239:Lysine
Verhalten der freien Aminosäuren im Serum von Ratten vor und nach Behandlung mit Urethan, Hexobarbital-Na und pentobarbital-Na. I. Mitteilung