Isolation and storage of progressively motile human sperm.
Human sperm progressively motile and free of seminal debris can routinely be isolated, during a 1.5 hour process, from semen of normal and subfertile men. Serum albumin, in a vertical column, acts to filter sperm based on their swimming abilities of forward progression. The isolated sperm constitute a population that can be inseminated as an aid to male subfertility or, to enhance the chances of sperm survival upon deep-freeze preservation. The latter use resulted in twice the survival rate, based on sperm motility, or random populations of control sperm.
D002470:Cell Survival; D005298:Fertility; D005615:Freezing; D006801:Humans; D008297:Male; D012710:Serum Albumin, Bovine; D013081:Sperm Motility; D013083:Sperm Transport; D013094:Spermatozoa; D013997:Time Factors
D012710:Serum Albumin, Bovine