[Thermolability at 56 degrees C of mouse IgE antibodies].
The heat inactivation at 56 degrees C of mouse IgE antibodies, measured by their PCA activity, was studied in various experimental conditions. Mouse IgE antibodies are partially protected against heat inactivation when previously diluted in sodium chloride or in phosphate buffer media. The protection is better at a higher dilution and molarity (phosphate 1M) and at pH 7. Heat inactivation is increased by the presence of reducing, alkylating and denaturating agents. Heat lability depends upon the concentration of serum proteins in the medium and is increased in presence of immunoglobulins.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D006358:Hot Temperature; D007073:Immunoglobulin E / Q000378:metabolism*; D051379:Mice
D000906:Antibodies; D007073:Immunoglobulin E
Effet du chauffage à 56 degrees C sur les antiborps IgE de la souris