Evidence that "embryonic estrogen" is a factor which controls the development of the mouse preimplantation embryo.
4- to 8-cell mouse PIE's (preimplantation embryos) were cultured in vitro for 48 hr in media containing various concentrations of the anti-estrogen CI-628. Graded effects of the various concentrations were observed with the highest concentration (1.5 microng/ml) being 100% effective in blocking development to the blastocyst stage. The deleterious effects of the drug were prevented to some extent by including estradiol-17beta in the medium. Our interpretation is that the effect of CI-628 was due to its anti-estrogenic properties. If this interpretation is correct, then the results support our hypothesis that the development of mouse PIE's depends upon estrogen which originates in the PIE.
D000818:Animals; D002455:Cell Division; D004622:Embryo, Mammalian / Q000187:drug effects / Q000502:physiology*; D047108:Embryonic Development; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000502:physiology*; D005260:Female; D051379:Mice; D011247:Pregnancy; D011270:Pregnancy, Animal
D004967:Estrogens; D004958:Estradiol