Covalent binding of foreign chemicals to tissue macromolecules.
In vivo and in vitro covalent binding of foreign chemicals to tissue macromolecules via metabolic activation is described, using the analgesic acetaminophen as an example. Acetaminophen is metabolized through a variety of pathways. The arylating metabolite is formed by a cytochrome P-450 dependent N-hydroxylation process. The resulting hydroxamic acid is then conjugated with glutathione, and the resulting conjugate is subsequently excreted as the mercapturic acid in the urine. It is not until the glutathione concentration is reduced to about 20% of the initial concentration that covalent binding of acetaminophen to amino acids of proteins occurs and subsequent liver necrosis is seen. The extent of in vitro binding correlates with treatments that alter hepatic necrosis and in vivo binding, indicating that in vitro binding is a valid index of acetaminophen hepatotoxicity. A simple bacterial test system for detecting chemical carcinogens as mutagens is described.
D000082:Acetaminophen / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000187:drug effects; D002273:Carcinogens; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats
D002273:Carcinogens; D009153:Mutagens; D004364:Pharmaceutical Preparations; D000082:Acetaminophen