Estimation of acetylcysteine and its preparations.
A sensitive, reproducible spectrophotometric procedure using Ellman's reagent for the determination of acetylcysteine in pharmaceutical dosage froms is presented. The method is based on the measurement of the absorbance of the anion of 2-nitro-5-thiolbenzoic acid at 412 nm, liberated as a result of the reaction of acetylcysteine and an alkaline solution of 5-5'-dithiobis-(2-nitrobenzoic acid). The optimum conditions for the reaction, speed of reaction and linearity relationship between concentration and absorbance are reported. It is concluded that the proposed method is simple, rapid and accurate for the determination of small quantities of acetylcysteine.
D000111:Acetylcysteine / Q000032:analysis*; D008027:Light; D008722:Methods; D012996:Solutions / Q000032:analysis; D013053:Spectrophotometry
D012996:Solutions; D000111:Acetylcysteine