Absence of significant secretory flux of phosphate in the proximal convoluted tubule.
The presence of phosphate secretion by the renal tubule in mammals has been controversial. Recently, in a micropuncture and microperfusion study, net secretion of phosphate was reported for the proximal tubule of rats which were infused with parathyroid hormone (PTH) and phosphate. Since the finding of net secretion of phosphate by the proximal tubule is in contradiction to other reports, the question was reinvestigated with the use of microperfusion techniques that were modified to allow identification of sample contamination. Studies were performed in intact dogs, phosphate- and PTH-loaded intact rats, and phosphate-loaded, acutely thyroparathyroidectomized rats. After exclusion of contaminated samples, no significant influx of phosphate was found in any of the three groups. Neither increased plasma phosphate concentration nor the infusion of parathyroid hormone unmasked a secretory process for phosphate. It is concluded that phosphate transport in the proximal tubule is essentially a unidirectional phenomenon, i.e., reabsorption is not opposed by significant backflux of phosphate.
D000818:Animals; D002118:Calcium / Q000097:blood; D004285:Dogs; D007444:Inulin / Q000378:metabolism; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008297:Male; D010280:Parathyroid Glands / Q000601:surgery; D010281:Parathyroid Hormone / Q000494:pharmacology; D010477:Perfusion; D010710:Phosphates / Q000378:metabolism*; D051381:Rats; D013965:Thyroidectomy
D010281:Parathyroid Hormone; D010710:Phosphates; D007444:Inulin; D002118:Calcium