Defluorination of methoxyflurane by a glutathione-dependent enzyme.
Partial purification from rat liver of an enzyme which catalyzes defluorination of methoxyflurane is described. Fractionation of liver homogenates by protamine sulfate and ammonium sulfate precipitation and by Sephadex G-100 chromatography results in a 10-fold purification with 53% recovery. The enzyme requires glutathione for activity, and other sulfhydrhyl compounds cannot be substituted. The enzyme appears to be a glutathione S-transferase, possibly one of several which have recently been characterized.
D000818:Animals; D005461:Fluorine / Q000378:metabolism; D005978:Glutathione; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008733:Methoxyflurane / Q000378:metabolism*; D051381:Rats; D013438:Sulfhydryl Compounds
D013438:Sulfhydryl Compounds; D005461:Fluorine; D008733:Methoxyflurane; D005978:Glutathione