Isolation of glycoprotein antigen from ascites fluid of pancreatic carcinoma.
A glycoprotein antigen from the ascites fluid of pancreatic carcinoma has been isolated with the use of perchloric acid extraction and chromatographies on Sepharose 4B, DEAE-Sephadex, and DE52, followed by Sephadex G-200. The purified glycoprotein was found to be homogenous by sodium dodecyl sulfate polyacrylamide gel electrophoresis. A molecular weight of 185,000 dattons was determined by gel filtration. The molecule contained 55% protein and 45% carbohydrate. Both the amino acid and carbohydrate compositions were different from those of carcinoembryonic antigen. This glycoprotein antigen of pancreatic cancer cross-reacted with anticarcinoembryonic antigen; however, the antiserum prepared from this antigen did not react with carcinoembryonic antigen. The biological significance of this glycoprotein in pancreatic cancer is being studied.
D000951:Antigens, Neoplasm / Q000032:analysis*; D001202:Ascitic Fluid / Q000276:immunology; D002272:Carcinoembryonic Antigen; D003429:Cross Reactions; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D006801:Humans; D008970:Molecular Weight; D010190:Pancreatic Neoplasms / Q000032:analysis / Q000276:immunology*
D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D006023:Glycoproteins