Differential cytotoxicity between transformed and normal human cells with combinations of aminonucleoside and hydroxyurea.
Normal human WI-38 cells can be protected from killing by hydroxyurea if proliferation is arrested during drug treatment. This protection was demonstrated both in cells arrested by density-dependent inhibition and by 3'-amino-3'deoxy-N6,N6-dimethyladenosine (puromycin aminonucleoside). In contrast, VA-13 cells (a simian virus 40-transformed clone of WI-38) were not arrested under these conditions, and continued to be sensitive to hydroxyurea. These results suggest that a search for agents that selectively and reversibly inhibit normal cycling human cells might lead to an enhancement of differential tumor toxicity.
D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D006918:Hydroxyurea / Q000494:pharmacology*; D011691:Puromycin / Q000031:analogs & derivatives*; D011692:Puromycin Aminonucleoside / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism
D011691:Puromycin; D011692:Puromycin Aminonucleoside; D012313:RNA; D013936:Thymidine; D006918:Hydroxyurea