[Biochemical changes in the kidney cells of dogs after the intravenous administration of deoxycholate sodium (the nephrotoxic action of amphotericin B)].
The effect of sodium desoxycholate on the plasmic membranes and chromatin of the dog kidney cells after its intravenous administration to the animals in a dose of 1.2 mg/kg equal to the content of sodium desoxycholate in the therapeutic preparation of amphotericin B once a day for 8 days was studied. It was shown that sodium desoxycholate did not induce any detactable shifts in the protein composition of the plasmic membranes. At the same time administration of sodium desoxycholate was accompanied by disorders in the fractions of the membrane-bound and free chromatin of the dog kidneys. It was found that sodium desoxycholate promoted binding of chromatin to the nuclear membrane, changed the chemical composition of the both fractions studied and damaged the spectrum of the histones  of free chromatin. An analogous character of the effect on the above subcellular structures of the kidney tissue was observed with the used of amphotericin B. However, the level of the effect of the antibiotic complex with sodium desoxycholate on the above structures was more significant than that of sodium desoxycholate used alone. The data obtained were indicative of sodium desoxycholate toxicity in vivo and promoted elucidatio  of the cause of amphotericin B toxic effect on microbial cells.
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002843:Chromatin / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004285:Dogs; D004338:Drug Combinations; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005609:Free Radicals; D006657:Histones / Q000032:analysis; D007275:Injections, Intravenous; D007668:Kidney / Q000187:drug effects* / Q000648:ultrastructure; D011485:Protein Binding / Q000187:drug effects
D002843:Chromatin; D004338:Drug Combinations; D005609:Free Radicals; D006657:Histones; D003840:Deoxycholic Acid; D000666:Amphotericin B; D004247:DNA
Nekotorye biokhimicheskie izeneniia v kletkakh pochek sobak posle vnutrivennogo vvedeniia dezoksikholata natriia (k voprosu o nefrotoksicheskom deĭstvii amfoteritsina B)