title: A new class of infectious agents detectable by the production of chorioallantoic membrane lesions by human lymphoblastoid cell lines and their culture supernatants.
abstract: Intravenous injection of cells and their tissue culture supernatants (CS) from human lymphoblastoid cell lines (LCL) induced the formation of lesions on the chorioallantoic membrane (CAM) of the chicken embryo. Injection of cells and CS from non-LCL and normal human lymphocytes induced few or no lesions. Irradiated chick embryos were more sensitive to lesion formation than were nonirradiated embryos. The log10 CAM lesions induced in irradiated (500 rads) embryos were a linear function of the log10 cells (from LCL) in the inoculum; the slope was 1.0, within experimental error. The formation of CAM lesions did not depend on the presence of Epstein-Barr virus (EBV) since lesions were also induced by cells and extracts derived from EBV genome-free LCL. Lesion-inducing activity associated with CS was filterable through 0.22-mu filters, sedimented at 78,000 x g, and sensitive to inactivation by heat (56 degrees C for 30 min), UV irradiation, chloroform, sera from chickens immunized against CS, and certain human sera. Lesion-inducing activity associated with cells and extracts was resistant to 5,000 rads of gamma-radiation. B2/B2 embryos (the B locus is the major histocompatibility locus of chickens) were more sensitive to lesion formation than were B15/B21 and outbred embryos; this suggested a genetic influence on lesion formation. Our data suggest that the irradiated chicken embryo may be a highly sensitive and useful means for the detection of an unidentified or unknown agent or agents that may play an important role in human oncogenic lymphocyte transformation or might interact with transforming viruses.
mesh_terms: D000482:Allantois / Q000473:pathology; D000818:Animals; D000914:Antibodies, Viral; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002051:Burkitt Lymphoma / Q000382:microbiology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo / Q000528:radiation effects; D002725:Chloroform / Q000494:pharmacology; D002823:Chorion / Q000473:pathology; D005321:Extraembryonic Membranes / Q000473:pathology*; D006358:Hot Temperature; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology; D007938:Leukemia / Q000382:microbiology; D008214:Lymphocytes / Q000382:microbiology*; D009858:Oncogenic Viruses / Q000302:isolation & purification*; D014466:Ultraviolet Rays; D014773:Virology / Q000379:methods*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000914:Antibodies, Viral; D002725:Chloroform; D001973:Bromodeoxyuridine; D007065:Idoxuridine