Acid phosphatase isoenzymes in Gaucher's disease.
Acid phosphatase (EC 3.1.3.2) isoenzyme profiles of extracts of splenic tissue and serum from patients with Gaucher's disease were measured by a mini-column ion-exchange chromatographic method [Clin. Chem., 23, 000 (1977)]. Diagnosis of Gaucher's disease in the five patients studied was confirmed by demonstrating decreased (2.3 to 4.1% of normal) glucocerebrosidase activity in the spleen. With p-nitrophenyl phosphate as substrate, increased acid phosphatase activity (three-to eight-fold normal) was demonstrated in spleen tissue from Gaucher;s disease patients; isoenzyme profiles by the ion-exchange column technique showed acid phosphatase isoenzyme 5 to be the predominant isoenzyme. Comparison of acid phosphatase isoenzyme profiles from patients with Gaucher's disease and prostatic carcinoma revealed distinct differences in the activities  of isoenzymes 2 and 5. The isoenzyme-5 measurement thus appears to provide a diagnostic test for Gaucher's disease that can be done reapidly and easily in the routine clinical chemistry laboratory.
D000135:Acid Phosphatase / Q000032:analysis* / Q000097:blood; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002852:Chromatography, Ion Exchange; D005260:Female; D005776:Gaucher Disease / Q000201:enzymology*; D005962:Glucosylceramidase / Q000032:analysis; D006801:Humans; D007223:Infant; D007527:Isoenzymes / Q000032:analysis*; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D013154:Spleen / Q000201:enzymology
D007527:Isoenzymes; D000135:Acid Phosphatase; D005962:Glucosylceramidase