A dialysis procedure for loading erythrocytes with enzymes and lipids.
A dialysis procedure for hypotonic hemolysis has been developed in which erythrocytes can be loaded with water-soluble enzymes, detergent-solubilized enzymes (glucocerebrosidase) and detergent-dispersed glycolipid (glucocerebroside). The procedure allows approx. 40-50% of the added enzyme or glycolipid to be encapsulated. The final intracellular concentration of enzyme or glycolipid is about to the extracellular concentration. The loaded cells can be ingested by macrophage in vitro and the glucocerebroside partially degraded by lysosomal glucocerebrosidase. The use of this procedure for the investogation of Gaucher's disease is discussed.
D002554:Cerebrosides / Q000097:blood* / Q000378:metabolism; D003840:Deoxycholic Acid / Q000494:pharmacology; D003956:Dialysis / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology; D005959:Glucosidases / Q000097:blood*; D005962:Glucosylceramidase / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D009994:Osmolar Concentration; D010587:Phagocytosis; D012995:Solubility
D002554:Cerebrosides; D003840:Deoxycholic Acid; D005959:Glucosidases; D005962:Glucosylceramidase