title: In vitro effects of rubella virus, strain RA 27/3, on human lymphocytes. I. Viral inhibition of mitogen stimulation in relation to Rubella Haemagglutination inhibition antibodies.
abstract: The inhibiting effect of rubella virus on lymphocyte stimulation in vitro was studied, using purified virus of the attenuated strain RA 27/3. Addition of the virus to human lymphocytes from twenty healthy blood donors before stimulation with leuco-agglutinin (LA), a component of phytohaemagglutinin, caused a considerable inhibition of the LA response in some experiments, whereas in other experiments the inhibition was slight or non-existent. If further analysed, the results showed a correlation between the degree of inhibition and the immunity of the lymphocyte donor against rubella, as measured by haemagglutination inhibition (HI). Thus the LA-response was significantly more depressed in a group of lymphocyte donors with HI-titres ranging from 20 to 160 than in another group with HI-titre less than 5. Possible explanations of the virus-induced inhibition of the LA response and possible connection between this phenomenon and the immune response against rubella are discussed.
mesh_terms: D000328:Adult; D000914:Antibodies, Viral; D002478:Cells, Cultured; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006387:Hemagglutination, Viral; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012412:Rubella virus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral