Squalene epoxidase and oxidosqualene lanosterol-cyclase activities in cholesterogenic and non-cholesterogenic tissues.
Squalene epoxidase and oxidosqualene lanosterol-cyclase activities have been studied in normal mammalian cholesterogenic and non-cholesterogenic tissues. This paper describes the kinetic conditions of measurement of these two enzymatic acitivities and their results. Oxidosqualene lanosterol-cyclase is a widespread enzyme, present in all cholesterogenic and non-cholesterogenic tissues. However, the level of squalene epoxidase is very low in non-cholesterogenic tissues. However, the level of squalene epoxidase is very low in non-cholesterogenic tissues. The effects of subcellular fractionizing and of the physico-chemical state of squalene incubated in vitro on squalene epoxidase activity are discussed.
D000818:Animals; D001921:Brain / Q000201:enzymology; D002784:Cholesterol / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D004988:Ethers, Cyclic; D005260:Female; D006801:Humans; D007535:Isomerases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D007810:Lanosterol; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology; D009928:Organ Specificity; D010105:Oxygenases / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D051381:Rats; D013185:Squalene; D013347:Subcellular Fractions / Q000201:enzymology
D004988:Ethers, Cyclic; D007810:Lanosterol; D013185:Squalene; D002784:Cholesterol; D010105:Oxygenases; D007535:Isomerases