Mechanisms for sister chromatid exchanges and their relation to the production of chromosomal aberrations.
By taking advantage of the fact that fluorescent light (FL) induces strand breaks only in bromodeoxyuridine(BrdUy-substituted DNA, and that those breaks eventually lead to the formation of sister chromatid exchanges (SCEs), the response of SCEs to FL was studied carefully in Chinese hamster chromosomes in which, out of four DNA strands, BrdU-substitution had occurred either in one or three strands. The FL-induced SCE frequency did not differ greatly between these two types of chromosomes. However, when they were submitted to caffeine treatment, a drastic increase in the frequency was detected in the trifilarly-substituted chromosomes while a significant decrease occurred in the unifilarly-substituted chromosomes. Based on these results, a working hypothesis was developed that the SCE can arise by at least two different mechanisms, one operating at replicating points probably utilizing the machinery of DNA replication, and the other acting only in the post-replicational DNA portion, probably in a similar fashion as assumed in a general model of crossing over in the eukaryote. These dual mechansims may account for the discrepancy encountered in the explanations of the induction of SCEs by various exogenous agents as well as spontaneous SCEs. The present study also showed that some, but clearly not all, of chromatid deletions are the outcome of the failure to complete SCEs arising through these mechanisms.
D001973:Bromodeoxyuridine; D002110:Caffeine / Q000494:pharmacology; D002478:Cells, Cultured; D002842:Chromatids / Q000528:radiation effects*; D002872:Chromosome Deletion; D003434:Crossing Over, Genetic / Q000528:radiation effects*; D004261:DNA Replication; D005453:Fluorescence; D010782:Photolysis
D002110:Caffeine; D001973:Bromodeoxyuridine