Early prostaglandin release from the ischemic myocardium in the dog.
Cellular consequences of myocardial ischemia were studied in anesthetized dogs. Confirmation of myocardial ischemia was provided by electrocardiographic and biochemical indexes. Prostaglandin F2alpha release into coronary venous blood was significantly elevated during myocardial ischemia, whereas indomethacin treatment prevented this increase in coronary venous prostaglandin F2alpha concentrations. No significant increase in prostaglandin E2 release was observed in response to myocardial ischemia, but indomethacin treatment significantly reduced coronary venous prostaglandin E2 concentrations below those of control values. Within one hour after occlusion of the coronary artery, the S-T segment was significantly altered, and coronary venous prostaglandin F2alpha had increased significantly above the control concentration. These changes persisted during four hours of myocardial ischemia. Plasma creatine phosphokinase activity increased significantly after two hours of myocardial ischemia and remained elevated for the subsequent two hours of ischemia. After four hours of myocardial ischemia, myocardial creatine phosphokinase activity of ischemic myocardium was significantly reduced, and labilization of myocardial treatment prevented increases in prostaglandin release but did not influence other biochemical changes or the electrocardiographic response to ischemia. Thus, prostaglandin release by ischemic myocardial tissue is an early response to the ischemic stimulus.
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002403:Cathepsins / Q000378:metabolism; D003327:Coronary Disease / Q000097:blood* / Q000201:enzymology / Q000503:physiopathology; D003331:Coronary Vessels; D003402:Creatine Kinase / Q000378:metabolism; D004285:Dogs; D004562:Electrocardiography; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D011458:Prostaglandins E / Q000097:blood*; D011460:Prostaglandins F / Q000097:blood*; D011863:Radioimmunoassay; D013997:Time Factors; D014666:Vasomotor System / Q000187:drug effects
D011458:Prostaglandins E; D011460:Prostaglandins F; D003402:Creatine Kinase; D002403:Cathepsins; D007213:Indomethacin