Further studies on the biological properties of guinea-pig IgG1 antibodies. III. Haemolytic efficiency in vitro.
The capacity of non-reciprocally contaminated guinea-pig IgG1 and IgG2 antibodies for sensitizing antigen-coated sheep erythrocytes for lysis was studied in the presence of serial dilutions of normal guinea-pig serum as the source of complement. IgG1 antibodies were highly efficient, provided complement was little diluted and not heated at 56 degrees. Chelation of Ca2+ by EGTA affected lysis of IgG1-sensitized cells by only 50%, while completely blocking lysis of IgG2-sensitized cells. The F(ab')2 fragment of IgG1, in contrast to the F(ab')2 fragment of IgG2, was almost as efficient as the parent antibody.
D000818:Animals; D000906:Antibodies; D002118:Calcium; D003165:Complement System Proteins; D006168:Guinea Pigs; D006461:Hemolysis; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D066298:In Vitro Techniques
D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D003165:Complement System Proteins; D002118:Calcium