Purification of rat intestinal receptor for intrinsic factor-vitamin B-12 complex by affinity chromatography.
Rat intrinsic factor was bound to vitamin B-12-Sepharose to produce intrinsic factor-vitamin B-12-Sepharose. Intestinal receptor for intrinsic factor-vitamin B-12 complex was purified from rat ileal extract by affinity chromatography using the intrinsic factor-vitamin B-12-Sepharose as an affinity adsorbent with recovery of 48.5% and specific activity increased 335 fold of original sample.
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification; D002846:Chromatography, Affinity; D005753:Gastric Mucosa; D007082:Ileum; D007437:Intrinsic Factor / Q000378:metabolism*; D008297:Male; D051381:Rats; D011955:Receptors, Drug / Q000302:isolation & purification*; D014805:Vitamin B 12 / Q000378:metabolism*
D002352:Carrier Proteins; D011955:Receptors, Drug; D007437:Intrinsic Factor; D014805:Vitamin B 12