Protoplast formation of selected Mycobacterium smegmatis mutants by lysozyme in combination with methionine.
Lysozyme-sensitive mutants of Mycobacterium smegmatis, isolated by nitrosoguanidine treatment, have been converted into protoplasts in a nutritionally enriched medium containing lysozyme and DL-methionine.
D008715:Methionine / Q000494:pharmacology*; D008722:Methods; D009113:Muramidase / Q000494:pharmacology*; D009154:Mutation; D009161:Mycobacterium / Q000187:drug effects / Q000648:ultrastructure*; D011523:Protoplasts
D008715:Methionine; D009113:Muramidase