Use of tetramethylbenzidine in plasma hemoglobin assay.
Plasma hemoglobin can be assayed by substituting 3,3',5,5'-tetramethylbenzidine for benzidine (a carcinogen) as the chromogenic reducing substance. Sensitivity, precision, and accuracy compare well with methods in which benzidine is used. Plasma inhibition can be accommodated by preincubation with hydrogen peroxide.
D001560:Benzidines; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008722:Methods
D001560:Benzidines; D006454:Hemoglobins