Biochemical applications of a quantitative high-pressure liquid chromatographic assay of warfarin and its metabolites.
A quantitative high-pressure liquid chromatographic assay of warfarin and its diastereoisomeric alcohols and 4'-, 6-, 7-, 8- and benzylic hydroxylated metabolites was accomplished using a reversed-phase, microparticle column (muBondapak/C18) employing 1.5% acetic acid (pH 4.7)-acetonitrile (69:31) as solvent. Concentration of these compounds on the column prior to their elution permitted the loading of 0.01-0.5 ml solutions of warfarin and metabolites without consequent alterations in peak heights and resolution. Details of the use of this technique in the study of R and S warfarin metabolism by hepatic mixed function oxidases and in the determination of warfarin and its metabolites in blood are presented.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D008297:Male; D008722:Methods; D008862:Microsomes, Liver / Q000032:analysis / Q000378:metabolism; D051381:Rats; D013237:Stereoisomerism; D014859:Warfarin / Q000032:analysis / Q000097:blood / Q000378:metabolism*
D014859:Warfarin