Cytoplasmic compartmentation of glucose 6-phosphate.
When rat diaphragm, intact or homogenized, was incubated with [14C]glucose, the initial specific activity of the fructose 6-phosphate was higher than that of glucose 6-phosphate (glc-6-P). This relationship was also found when the cytosol was incubated with the labeled glucose. The early time course of production of glc-6-P from glucose and its removal provide evidence that newly synthesized glc-6-P does not equilibrate rapidly with the total pool of this metabolite and is preferentially removed by isomerization. It is concluded that glc-6-P is functionally compartmented within the cytoplasm.
D000818:Animals; D002458:Cell Fractionation; D003600:Cytosol / Q000378:metabolism; D003964:Diaphragm / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005958:Glucosephosphates / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008931:Mitochondria, Muscle / Q000378:metabolism; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism
D005958:Glucosephosphates; D005947:Glucose