Studies on the chemistry of human platelet factor 4.
The chemical characteristics of several PF4 preparations have been examined by gel electrophoresis, amino acid analysis and NH2-terminal amino acid sequence determination. Preparations of PF4 from gel filtration and from affinity chromatography appeared identical. A single NH2-terminal sequence was determined as follows: NH2-Glu-Ala-Glu-Glu-Asp-Gly-Asp-Leu-Gin-SCMCys-Leu-SCMCys-Val-Lys-Thr-Thr-Ser-Gln-Val-Arg-. The molecular weight of the PF4 subunits, which seem to be identical, was 7,100 based on the recovery of NH2-terminal glutamic acid.
D000595:Amino Acid Sequence; D001779:Blood Coagulation Factors / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008968:Molecular Conformation; D008970:Molecular Weight; D010446:Peptide Fragments; D010978:Platelet Factor 4 / Q000302:isolation & purification
D001779:Blood Coagulation Factors; D010446:Peptide Fragments; D010978:Platelet Factor 4