title: Changes in sensitivity to radiation and ICRF 159 during the life of monolayer cultures of EMT6 tumour line.
abstract: The response of EMT6 mouse tumour cells to ICRF 159, both with and without X-radiation, has been measured during the life of monolayer cultures. The cytotoxic effect of ICRF 159 was found to be proliferation-dependent. Flow cytofluorimetry studies of cell cycle distribution showed that ICRF 159 prevented cell division while allowing DNA synthesis to continue. This anti-mitotic action and the cytotoxic effect of the drug were found to be closely related. Increased sensitivity to X-radiation was observed in cultures pretreated for 24 h with 200 microgram/ml ICRF 159 In exponential and early plateau cultures this was seen as a reduced shoulder of the survival curve. In late plateau cultures there was no apparent reduction of the shoulder, but an increase in slope.
mesh_terms: D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D009374:Neoplasms, Experimental / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology*; D011929:Razoxane / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D010879:Piperazines; D011929:Razoxane; D004247:DNA