Determination of the configurations of lactic and glyceric acids from human serum and urine by capillary gas-liquid chromatography.
The separation of the enantiomers of lactic and glyceric acids can be achieved by capillary gas chromatography on SP-1000 using the corresponding O-acetylated methyl esters. The structures of the derivatives were proved by proton magnetic resonance spectroscopy and mass spectrometry. The method has been used for the determination of the absolute configuration of lactic and glyceric acids isolated from serum and urine from different patients.
D002197:Capillary Action; D002849:Chromatography, Gas / Q000379:methods*; D002853:Chromatography, Liquid; D005988:Glyceric Acids / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D007773:Lactates / Q000032:analysis* / Q000097:blood / Q000652:urine; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013237:Stereoisomerism
D005988:Glyceric Acids; D007773:Lactates