Age-related changes of accuracy and efficiency of protein synthesis machinery in rat.
The accuracy and the efficiency of the protein synthesis machinery has been measured by the variability of the [3H]leucine/[14C]phenylalanine ratio in a cell-free protein synthesis system, poly(U) directed, and with the same criteria by binding aminoacyl tRNA-ribosome complexes with poly(U). Using these two methods, we have observed the aging of rats to be characterized by an increase of 3H/14C ratios according to the "general error theory". The results obtained are discussed in relation to the redundancy and rectification of the genes involved in the protein synthesis machinery formation.
D000375:Aging; D000818:Animals; D002250:Carbon Radioisotopes; D007553:Isotope Labeling; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000254:growth & development / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D011072:Poly U; D014176:Protein Biosynthesis; D051381:Rats; D012270:Ribosomes / Q000378:metabolism*; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D011072:Poly U; D010649:Phenylalanine; D007930:Leucine