Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase.
An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified. Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa-2) has a doubling time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa-2 grows in soft agar with a colony-forming efficiency of 19%. Both MIA PaCa-2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.
D000368:Aged; D000782:Aneuploidy; D001215:Asparaginase / Q000494:pharmacology*; D002330:Carmustine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology; D005342:Fibrinolysis / Q000187:drug effects; D006801:Humans; D008297:Male; D010190:Pancreatic Neoplasms / Q000235:genetics; D010958:Plasminogen / Q000096:biosynthesis; D014750:Vincristine / Q000494:pharmacology
D003561:Cytarabine; D014750:Vincristine; D010958:Plasminogen; D001215:Asparaginase; D002330:Carmustine