Effect of 2-mercaptoethanol on the in vitro response of sensitized bovine peripheral blood lymphocytes to Anaplasma antigen.
Sensitized lymphocytes from 3 cows vaccinated with anaplasma antigen were isolated and caused to react in vitro with antigen in a microculture lymphocyte transformation test. The addition of 2-mercaptoethanol at a concentration of 25 mul of 4 X 10(-3) M solution/well significantly (P less than or equal to 0.01) increased [3H]thymidine incorporation compared to microculture without 2-mercaptoethanol.
D000709:Anaplasma / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial; D002417:Cattle / Q000276:immunology*; D005260:Female; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects; D008623:Mercaptoethanol / Q000494:pharmacology*
D000942:Antigens, Bacterial; D008623:Mercaptoethanol