Detection reagent for adenine, guanine, uracil, cytosine and their alkylated bases, nucleotides and nucleosides on thin-layer plates.
The detection of alkylated bases at the nanogram level is reported. The reaction is based on two steps, first, chlorination of the bases and, secondly, spraying the chlorinated product with o-tolidine solution to give a dark blue color. The detection of nucleotides and nucleosides is also possible. The optimum conditions and the possible mechanism of the reaction are discussed.
D000225:Adenine / Q000032:analysis*; D000478:Alkylation; D002713:Chlorine; D002855:Chromatography, Thin Layer; D003596:Cytosine / Q000032:analysis*; D006147:Guanine / Q000032:analysis*; D007202:Indicators and Reagents; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D014498:Uracil / Q000032:analysis*
D007202:Indicators and Reagents; D009705:Nucleosides; D009711:Nucleotides; D002713:Chlorine; D014498:Uracil; D006147:Guanine; D003596:Cytosine; D000225:Adenine