Concanavalin A receptors on the surfaces of human breast cancer cells in organ culture.
The binding of concanavalin A (Con A) at the free apical membranes of surface tumor cells in human breast cancer explants grown in organ culture was studied cytochemically with horseradish peroxidase (HRP) as a marker. On the cell membranes in aldehyde-fixed explants or explants exposed to Con A at 4 degrees C, a continuous label covered the entire free surface of the cell, which indicated the dispersed distribution of Con A binding sites. The binding of Con A at 20 degrees C resulted in discontinuous label of the cell surface, with gaps of unlabeleled membrane and partial endocytosis of the label. Incubation at 37 degrees C, following the binding of Con A and HRP at both temperatures, induced more extensive, incubation time-dependent discontinuities of the surface label that led to complete disappearance of the label from the surface and its eventual endocytosis. Con A was topically stablized on the surface only in those regions where two membranes or a different part of a folded membrane were in close contact. No differences were found in the binding, redistribution, and internalization of bound Con A among the various tumors studied.
D001943:Breast Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D004705:Endocytosis; D005260:Female; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D009924:Organ Culture Techniques; D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D013696:Temperature; D013997:Time Factors
D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D006735:Horseradish Peroxidase