The estimation of lymphotoxic activity of ALS by spectrophotometric method.
Saijo's method of quantitation of the cytotoxicity of antiserum for tumor cells is adapted to estimation of lymphotoxic activity of antilymphocyte sera. The method is based on spectrophotometric measurement of an amount of trypan blue dye entering the lymphocytes damaged by action of ALS. Several possibilities of determination of the lymphotoxic titers of ALS are suggested.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D003165:Complement System Proteins / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D004306:Dose-Response Relationship, Immunologic; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013053:Spectrophotometry; D013696:Temperature; D014343:Trypan Blue
D000961:Antilymphocyte Serum; D003165:Complement System Proteins; D014343:Trypan Blue