Bee venom peptides XVIII. Peptide-m and mcd-peptide: Isolation and characterization.
A simple method is described for the purification of peptide-M and MCD-peptide from bee venom. The steps are Sephadex G-50 gel filtration of pH 4.5 (Steps 1 and 2) and chromatography on silica gel (Steps 3 and 4). The peptides are homo geneous with respect to thin-layer chromatography, electrophoresis at pH 2.0, and amino acid analysis. Peptide-M contains 24 amino acid residues and has a calculated molecular weight of 2750.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001516:Bees; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004588:Electrophoresis, Cellulose Acetate; D008722:Methods; D010455:Peptides / Q000302:isolation & purification*; D014688:Venoms / Q000032:analysis*
D000596:Amino Acids; D010455:Peptides; D014688:Venoms