Characterization of an immunogenic fraction of Pasteurella multocida culture filtrates.
An immunogenic fraction (IF) of Pasteurella multocida strain P-1059 was separated from culture filtrate by Sephadex gel filtration. Additional fractionation of IF with aqueous ether resulted in the glycoprotein-like preparation (GLP) while extraction with aqueous phenol provided the lipopolysaccharide-like preparation (LPP). The unextracted IF contained carbohydrate, protein, and lipid; the GLP contained carbohydrate and protein; and the LPP contained carbohydrate and lipid. The GLP was maximally protective for mice against homologous challenge, and was medially toxic in rabbit skin when compared to the other culture-filtrate preparations; the LPP was maximally toxic in rabbit skin, and was least protective for mice; and the unextracted IF was medially protective for mice, and was least toxic in rabbit skin.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000633:toxicity; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology; D007114:Immunization; D008055:Lipids / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology; D008297:Male; D051379:Mice; D010325:Pasteurella / Q000276:immunology*; D010326:Pasteurella Infections / Q000517:prevention & control; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000276:immunology; D011817:Rabbits
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002241:Carbohydrates; D006023:Glycoproteins; D008055:Lipids; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial