Esterification of trifluoroacetic acid with phenyldiazomethane for quantitative gas chromatographic analysis. Methods involving separation from biological materials.
Trifluoroacetic acid in biological materials has been quantitatively determined by gas chromatography. Benzyl trifluoroacetate has been prepared by the reaction of the acid with phenyldiazomethane, and has been successfully analyzed by gas chromatography without any interference from other peaks. The procedure has been used to determine trifluoroacetic acid in microsomal suspension incubated with halothane, a gaseous anaesthetic.
D000818:Animals; D002849:Chromatography, Gas; D003978:Diazomethane / Q000031:analogs & derivatives; D004951:Esterification; D005463:Fluoroacetates / Q000032:analysis*; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D008722:Methods; D008862:Microsomes, Liver / Q000032:analysis; D011485:Protein Binding; D011817:Rabbits; D013997:Time Factors; D014269:Trifluoroacetic Acid / Q000032:analysis*
D005463:Fluoroacetates; D003978:Diazomethane; D014269:Trifluoroacetic Acid