Use of electrophoresis in the identification and quantitation of antibiotics administered in combinations.
The investigation presents a method of electrophoretic separation of antibacterial drugs which are used in combinations in clinical medicine. Subsequent to electrophoresis in agarose gel, a microbiological assay was performed. This technique permitted the determination of the concentrations of beta-lactam antibiotics, rifampicin, and clindamycin in the presence of aminoglycosides. In therapeutic combinations of fusidic acid and clindamycin, the concentrations of each drug could be determined.
D000617:Aminoglycosides / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification*; D002228:Carbenicillin / Q000302:isolation & purification; D002981:Clindamycin / Q000302:isolation & purification; D004338:Drug Combinations; D004586:Electrophoresis; D004587:Electrophoresis, Agar Gel; D005672:Fusidic Acid / Q000302:isolation & purification; D005839:Gentamicins / Q000302:isolation & purification*; D007769:Lactams / Q000302:isolation & purification; D012293:Rifampin / Q000302:isolation & purification
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004338:Drug Combinations; D005839:Gentamicins; D007769:Lactams; D002981:Clindamycin; D005672:Fusidic Acid; D002228:Carbenicillin; D012293:Rifampin