Mitochondrial DNA polymerase from rat liver.
A DNA-dependent DNA polymerase from rat liver mitochondria was partially purified and characterized. Mitochondrial DNA polymerase has been found to be quite different from other DNA-dependent DNA polymerases alpha and beta present in the rat liver in the following points: elution patterns in a DEAE-cellulose column chromatography, sedimentation coefficients determined by the glycerol gradient centrifugation in the presence of high salt, and sensitivities to N-ethylmaleimide, ethidium bromide and KCl.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004256:DNA Polymerase I / Q000302:isolation & purification / Q000378:metabolism; D004257:DNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000378:metabolism; D004789:Enzyme Activation; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000648:ultrastructure; D008970:Molecular Weight; D051381:Rats
D004272:DNA, Mitochondrial; D008345:Manganese; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase; D008274:Magnesium; D002118:Calcium