title: Effect of preimmunization with native and methylated carrier on class-specific anti-hapten and anti-carrier antibody response.
abstract: Effects of priming with bovine serum albumin (BSA) and methylated BSA (MBSA) on the primary anti-2,4-dinitrophenyl (DNP) response were studied in the chicken. A new radioimmunoassay of class-specific antibodies (RIACA) was used to quantitate IgM and IgG antibodies against BSA and DNP. The anti-DNP antibody response after priming with BSA was dose-dependent: large doses of BSA suppressed and the smallest dose augmented it. In contrast, no dose dependence was observed between MBSA and the anti-hapten response. All doses of MBSA enhanced the primary anti-DNP antibody response of both immunoglobulin classes. No corresponding differences in anti-BSA antibodies were observed between BSA- and MBSA-primed groups. This suggests that the anticarrier antibodies as such are not the agent suppressing the anti-hapten response when large doses of native carrier are used for priming. A more probable explanation of this phenomenon is a competition between carrier-specific B cells and hapten-specific B cells for common T cells. A less obvious explanation is the generation of more helper cells or less suppressor cells when priming with MBSA than with BSA.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000276:immunology*; D002645:Chickens; D004140:Dinitrophenols / Q000276:immunology; D006241:Haptens; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008745:Methylation; D012710:Serum Albumin, Bovine / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D004140:Dinitrophenols; D006241:Haptens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012710:Serum Albumin, Bovine