Quantitative determination of albumin dimer in albumin preparations by combined starch-gel electrophoresis and immunoprecipitation.
A method is described for albumin dimer determination. The method is based upon an electrophoretic separation of albumin dimer from the monomer in starch gel with a subsequent immunochemical diffusion and precipitation of the albumin components in agarose gel containing antibodies. The precipitation zones are measured and compared iwth those of albumin monomer standards. A factor for converting monomer to dimer has furthermore been established.
D000418:Albumins / Q000032:analysis*; D004592:Electrophoresis, Starch Gel; D006801:Humans; D008722:Methods; D010602:Pharmacopoeias as Topic; D011233:Precipitin Tests; D012709:Serum Albumin / Q000032:analysis*
D000418:Albumins; D012709:Serum Albumin