Activation of the rat liver glucocorticoid--receptor complex.
The rat liver glucorcorticoid receptor has been activated using three procedures: heat, gel filtration, and dilution. With time after heat activation the steroid--receptor complex loses its capacity to bind to DNA--cellulose, while receptor activated by Sephadex G-25 and by dilution maintains DNA--cellulose binding capacity. The rates of steroid dissociation from nonactivated and activated receptor and essentially identical. However, nonactivated receptor is capable of rebinding steroid, while activated receptor has a reduced capacity to rebind steroid. The results of the gel filtration and dilution studies suggest that a low-molecular-weight factor(s) exists in rat liver cytosol which is involved in the process of activation.
D000315:Adrenalectomy; D000818:Animals; D001667:Binding, Competitive; D002482:Cellulose; D003600:Cytosol / Q000378:metabolism; D004247:DNA; D003907:Dexamethasone / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D012685:Sepharose
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone; D002482:Cellulose; D004247:DNA; D012685:Sepharose