Characteristics of the insulin stimulation of DNA, RNA and protein metabolism in cultured human mammary carcinoma cells.
In a mammary gland cell line (MCF-7) of human origin, insulin stimulated the rates of RNA, DNA and protein biosynthesis. These effects were observed with concentrations of insulin ranging from 10(-6) M to 10(-10) M. Enhanced rates of [3H]leucine incorporation into protein and [3H]uridine incorporation into RNA were observed within 1 h after exposing the cells to insulin. In contrast, a stimulatory effect of insulin on the rate of [3H]thymidine incorporation into DNA was only detectable following a 12--16 h incubation with insulin. Insulin also enhanced the rate of uptake of [3H]leucine, [3H]uridine, and [3H]thymidine into the MCF-7 cells. Finally, incubation with insulin increased the total amount of DNA, RNA and protein in these cells.
D001943:Breast Neoplasms; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D009363:Neoplasm Proteins / Q000096:biosynthesis; D014176:Protein Biosynthesis / Q000187:drug effects*; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects*
D004273:DNA, Neoplasm; D007328:Insulin; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm