Preparation of high-quality iodine-125-labelled pituitary human follicle-stimulating hormone (hFSH) for radioimmunoassay: comparison of enzymatic and chloramine-T iodination.
A method is described for the enzymatic radioiodination of human follicle-stimulating hormone (hFSH) by a system consisting of lactoperoxidase, hydrogen peroxide and Na125I. It was compared with the Chloramine-T modified technique. A satisfactory specific activity of the labelled hormone was obtained with the enzymatic iodination with much greater immunoreactivity was stability than after Chloramine-T.
D000918:Antibody Specificity; D002700:Chloramines; D002850:Chromatography, Gel; D004592:Electrophoresis, Starch Gel; D005640:Follicle Stimulating Hormone / Q000032:analysis*; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D007784:Lactoperoxidase; D008722:Methods; D010544:Peroxidases; D011863:Radioimmunoassay
D002700:Chloramines; D007457:Iodine Radioisotopes; D005640:Follicle Stimulating Hormone; D007784:Lactoperoxidase; D010544:Peroxidases