A new serum alpha-amylase assay of high sensitivity.
The use of chemically modified starch substrates for measurement of serum alpha-amylase activity is described. Action of alpha-amylase on such substrates, in the presence of excess fungal glucoamylase, results in the production of glucose in direct proportion to the amount of alpha-amylase present. The glucose produced is measured by a specific enzymic assay. Results obtained by using this new assay correlate well with activities determined by a conventional saccharogenic assay. The new method is of much higher sensitivity, and is less susceptible to interference, than are most other alpha-amylase assay methods.
D000681:Amylases / Q000097:blood* / Q000378:metabolism; D000688:Amylose / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D006868:Hydrolysis; D008722:Methods; D010084:Oxidation-Reduction; D012463:Saliva / Q000201:enzymology; D013213:Starch / Q000378:metabolism; D013997:Time Factors
D013213:Starch; D000688:Amylose; D000681:Amylases; D005959:Glucosidases