title: Nitrogen retention in rats fed on diets enriched with arginine and glycine. 2. Effect of diethyl ether anaesthesia on N retention.
abstract: 1. Adult rats were subjected to a brief period of diethyl ether anaesthesia and were given diets with 200 or 100 g casein/kg with or without arginine plus glycine supplementation in the post-anaesthesia period. Nitrogen retention was measured as well as liver protein content and liver and muscle transaminase activities (L-aspartate aminotransferase (GOT), (EC 2.6.1.1), and L-alanine aminotransferase (GPT)(EC 2.6.1.2). 2. Results demonstrated that anaesthesia-stressed rats consuming the high-protein diet with supplemental arginine and glycine retained twice as much N as did rats given the diet with 200 g casein/kg alone, for the first 5 d post-anaesthesia. 3. Anaesthesia-stressed animals consuming the diets with 100 g casein/kg with or without arginine plus glycine supplementation did not differ from each other in N retention. 4. Liver protein content increased after anaesthesia in rats given the high-protein diets; liver transaminase activity increased, whereas muscle transaminase activity decreased, in animals consuming the high protein diets. 5. Possible mechanisms to account for these results are discussed.
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000768:Anesthesia, General; D000818:Animals; D001120:Arginine / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D004986:Ether; D005019:Ethyl Ethers; D005527:Food, Fortified; D005998:Glycine / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D009584:Nitrogen / Q000378:metabolism*; D009747:Nutritional Physiological Phenomena; D011506:Proteins / Q000378:metabolism; D051381:Rats
chemical_list: D005019:Ethyl Ethers; D011506:Proteins; D004986:Ether; D001120:Arginine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D009584:Nitrogen; D005998:Glycine