Antibody-mediated suppression of tumor growth. I. Suppression by murine IgG1 isolated from alloantiserum.
The tumor suppressive activity of murine IgG1 antibody was studied in vivo. IgG1 was isolated from hyperimmune alloantisera against a murine lymphoma, 6C3HED, by absorption with heat-killed, formalinized Staphylococcus aureus. Cowan strain I, followed by DEAE ion exchange chromatography and Sephadex G-200 gel filtration chromatography. The isolated IgG1, which had no detectable IgM, IgA, IgG2a, IgG2b or IgG3, could suppress the growth of the tumor. In addition, DEAE and Sephadex G-200 column profiles of the in vivo tumor suppressive activity showed good correlation with the profiles of total IgG1 immunoglobulin and anti-tumor antibody assayed for IgG1 heavy chains and kappa light chains. The IgG1 tumor suppressive activity was not diminished after heating at 56 degrees C for 30 min.
D000818:Animals; D000918:Antibody Specificity; D002471:Cell Transformation, Neoplastic; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006358:Hot Temperature; D007106:Immune Sera; D007074:Immunoglobulin G / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D007166:Immunosuppressive Agents; D007518:Isoantibodies / Q000032:analysis; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011863:Radioimmunoassay
D007106:Immune Sera; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D007518:Isoantibodies