Synthesis of new steroid haptens for radioimmunoassay. Part III. 15beta-Carboxyethylmercaptosteroid-bovine serum albumin conjugates. Specific antisera for radioimmunoassay of 5alpha-dihydrotestosterone, 5alpha-androstane-3beta, 17beta-diol and 5alpha-androstane-3alpha, 17beta-diol.
The syntheses of 15beta-carboxyethylmercapto-5alpha-dihydrotestosterone, 15beta-carbosyethylmercapto-5alpha-androstane-3beta, 17beta-diol and 15beta-carboxyethylmercapto-5alpha-androstane-3alpha, 17beta-diol and the preparation of their bovine serum albumin (BSA) conjugates are described. These conjugates were employed for the generation of specific antisera suitable for radioimmunoassay (RIA) of 5alpha-dihydrotestosterone (5alpha-DHT), 5alpha-androstane-3beta, 17beta-diol (3beta-diol) and 5alpha-androstane-3alpha, 17beta-diol (3alpha-diol).
D015113:Androstane-3,17-diol / Q000031:analogs & derivatives / Q000032:analysis* / Q000276:immunology; D000731:Androstanes / Q000032:analysis*; D003429:Cross Reactions; D013196:Dihydrotestosterone / Q000031:analogs & derivatives / Q000032:analysis* / Q000276:immunology; D006241:Haptens; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods
D000731:Androstanes; D006241:Haptens; D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol