Development and characterization of cell lines of normal mouse bladder epithelial cells and 2-acetylaminofluorene-induced urothelial carcinoma cells grown in monolayer tissue culture.
Normal urinary bladder epithelial cells and cells derived from 2-acetylaminofluorene-induced urothelial carcinomas from male Balb/c mice were grown in monolayer culture and were characterized. Cell lines of normal bladder epithelium were mononucleated, sheet-forming cells, with a modal chromosome number of 40. Bladder epithelial carcinoma cells induced by 2-acetylaminofluorene were polynucleate, relatively fast growing, grew in soft agar, demonstrated a higher cloning efficiency than normal cells and formed tumors when inoculated into syngeneic hosts. Differences in morphology were recorded by photomicrography using phase optics and scanning electron microscopy.
D015073:2-Acetylaminofluorene; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology / Q000648:ultrastructure*; D001743:Urinary Bladder / Q000648:ultrastructure*
D000951:Antigens, Neoplasm; D015073:2-Acetylaminofluorene