Radioimmunoassay of PGE and an approach to the specific measurement of PGE1.
A specific radioimmunoassay for prostaglandins of the E series is presented. Several refinements of the conventional procedures used for unknown sample preparation prior to assay are described. Validation of the method through recovery of labeled and unlabeled prostaglandin E from plasma indicates: 74% extraction efficiency, 80% chromatographic separation efficiency, and a 67% overall recovery figure for the complete assay. The development of an "absorbed system" approach for measuring one E prostaglandin (PGE1) without interference from the other E prostaglandin (PGE2) is also described. A preliminary study of rat tissue and male plasma samples measured by these assay systems is included. Alternate approaches to the development of a specific PGE1 assay, although less feasible in these authors' hands, are discussed.
D000818:Animals; D001923:Brain Chemistry; D002845:Chromatography; D046508:Culture Techniques; D006801:Humans; D007106:Immune Sera; D007668:Kidney / Q000032:analysis; D008168:Lung / Q000032:analysis; D008297:Male; D011458:Prostaglandins E / Q000032:analysis / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D012824:Silicic Acid
D007106:Immune Sera; D011458:Prostaglandins E; D012824:Silicic Acid