The action of N-hydroxy-2-acetylaminofluorene on the synthesis of ribosomal and poly (A)-RNA in normal and regenerating liver.
Polyribosomal RNA prepared from normal and regenerating liver was chromatographed on poly(U)-Sepharose to obtain rRNA and poly(A)-RNA. Under the conditions employed for labeling the RNA with [5-3H]orotic acid, poly (A)-RNA from regenerating liver represented 1--3% of the total RNA content, and 14--18% of the total radioactivity in polyribosomal RNA at varying times after partial hepatectomy. The specific activity of poly (A)-RNA was elevated by 50% and 30% only at 2 h and 6 h after partial hepatectomy, respectively. Ribosomal RNA synthesis increased 2.5--4 fold at 2--20 h after operation. Treatment of normal or partially hepatectomized rats with N-hydroxy-2-acetylaminofluorene showed a differential dose-dependent effect on rRNA and poly (A)-RNA synthesis with rRNA synthesis being more sensitive to the inhibitory effect of the carcinogen. No change in the poly(A) content was apparent as a result of N-hydroxy-2-acetylaminofluorene treatment.
D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005449:Fluorenes / Q000494:pharmacology*; D006498:Hepatectomy; D006881:Hydroxyacetylaminofluorene / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D008115:Liver Regeneration; D008297:Male; D011061:Poly A; D011132:Polyribosomes / Q000378:metabolism; D012335:RNA, Ribosomal / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats
D005449:Fluorenes; D012335:RNA, Ribosomal; D003609:Dactinomycin; D011061:Poly A; D006881:Hydroxyacetylaminofluorene