Conformations of purine ribosyl 5'-nucleotides bound to glycogen phosphorylase b. A proton T2 relaxation time investigation.
The conformation of 5'-nucleotides in the active site of glycogen phosphorylase b has been deduced from linewidth measurements of protons H-1', H-8 and H-2. It is shown by selective deuteration of the purine ring in position 8 that the orientation of the base is anti in the case of strong activators like AMP and syn in that of weak activators like IMP. The orientation correlation time of the nucleotides in the active site is nearly that of the enzyme, i.e. 160 ns at 21 degrees C.
D000249:Adenosine Monophosphate / Q000032:analysis; D000818:Animals; D007292:Inosine Nucleotides / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008433:Mathematics; D008968:Molecular Conformation; D009132:Muscles / Q000201:enzymology; D006005:Phosphorylases; D011487:Protein Conformation; D011817:Rabbits
D007292:Inosine Nucleotides; D000249:Adenosine Monophosphate; D006005:Phosphorylases