[Use of affinity chromatography for isolation of antibodies to human brain-specific antigens and several findings on the characteristics of these antigens].
With the aid of affinity chromatography on sepharose-4B, activized by CNBr from the blood serum of rabbits who were previously immunized by general phospate protein extract of the human brain, the author identified antibodies to brainspecific antigens. The specificity of the directness of the obtained antibodies was proved by the reaction of double diffusion in agar gel. With the aid of the same antigens by the immunoelectrophoresis method two brainspecific antigens of a glycoproteid nature with an electrophoretic mobility alpha1 and alpha2-globulins were detected. A processing of the same immunophoregrams by labeled 125i antibodies to rabbit gamma-globulins permitted to find another brainspecific antigen with a mobility beta2-gamma-globulin.
D000906:Antibodies / Q000302:isolation & purification*; D000941:Antigens; D001921:Brain / Q000276:immunology*; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007122:Immunoelectrophoresis; D009419:Nerve Tissue Proteins / Q000276:immunology; D009928:Organ Specificity
D000906:Antibodies; D000941:Antigens; D006023:Glycoproteins; D009419:Nerve Tissue Proteins
Ispol'zovanie metoda affinnoĭ khromatografii dlia vydeleniia antitel k mozgospetsificheskim antigenam cheloveka i nekotorye danny k kharakteristikeétikh antigenov.