Endothelial adenine uptake as an assay for cell- or complement-mediated cytotoxicity.
[3H] Adenine uptake by monolayers of cultured pig aortic endothelium was characterized and used as the basis for a terminal adenine uptake cytotoxicity assay. This assay was used to demonstrate specific cytotoxicity against donor-type endothelium by serum and by peripheral blood lymphocytes from skin-graft allergized pigs, and to compare the time course of these two types of effector mechanism.
D000225:Adenine / Q000378:metabolism*; D000818:Animals; D000937:Antigen-Antibody Reactions; D001011:Aorta / Q000378:metabolism; D002478:Cells, Cultured; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D004727:Endothelium / Q000378:metabolism*; D008214:Lymphocytes / Q000276:immunology; D013552:Swine; D013997:Time Factors
D003165:Complement System Proteins; D000225:Adenine