Calcium-induced displacement of membrane-associated particles upon aggregation of chromaffin granules.
Isolated chromaffin granules incubated in 10 millimolar calcium chloride aggregated, forming contact sites with a pentalaminar membrane structure. These circular attachment sites were free of membrane-associated particles, which accumulated at the periphery. Incubation in 20 millimolar ethylenediaminetetraacetic acid reversed these changes, which are regarded as initial events in the membrane fusion reaction.
D000313:Adrenal Medulla / Q000502:physiology / Q000648:ultrastructure*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle; D002462:Cell Membrane / Q000648:ultrastructure; D005089:Exocytosis; D005614:Freeze Fracturing; D008566:Membranes / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron
D002118:Calcium