IR spectrophotometric assay of carbachol solutions.
Carbachol ophthalmic solutions can be assayed by evaporating a measured volume, dissolving the residue in methanol, and scanning the carbonyl stretching frequency in an IR spectrophotometer using a cell with calcium fluoride windows. Methylcellulose and other formulation vehicle components do not interfere. The method is stability indicating with respect to hydrolysis. It affords a recovery of 99.5+/-0.51% (RSD).
D002217:Carbachol / Q000032:analysis*; D008722:Methods; D009883:Ophthalmic Solutions; D013055:Spectrophotometry, Infrared
D009883:Ophthalmic Solutions; D002217:Carbachol