A simple method for detection of IgG rheumatoid factor.
A simple method for detection of IgG rheumatoid factor (RF) in sera and joint fluid is described. The technique is based on the action of 0.1 M 2-mercaptoethanol mixed directly with the material. After 2 h of incubation determinations of human anti-IgG were performed by latex agglutination test. Comparison with data obtained by using the conventional method, sequential 24 h treatment with 0.1 M 2-mercaptoethanol and 0.01 M iodoacetamide, shows similar results for both methods. A correlation was observed between the presence of IgGRF in synovial fluid and a severe clinical course with invalidating forms in patients suffering rheumatoid arthritis.
D000936:Antigen-Antibody Complex; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001323:Autoantibodies; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007841:Latex Fixation Tests; D008297:Male; D008623:Mercaptoethanol; D012217:Rheumatoid Factor / Q000032:analysis*; D013582:Synovial Fluid / Q000276:immunology
D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D007074:Immunoglobulin G; D008623:Mercaptoethanol; D012217:Rheumatoid Factor