The role of dog bladder mucosa in the N-oxidation of arylamines.
The in vitro metabolic N-oxidation of 1- and 1-napthylamine, 4-biphenylamine, 2-fluorenylamine and 3-dibenzolfuranylamine has been investigated with intact dog bladder, whole intact bladder mucosa and microsomes prepared from this tissue. Very low levels of metabolic N-oxidation of these carcinogenic amines were detected with these tissue preparations using ferrihemoglobin formation in dog erythrocytes. No N-oxidation by these tissue preparations was observed using gas-liquid chromatography. The concentrations of the N-oxidized metabolites observed in the urine of dogs in vivo exposed to thse amines suggests that N-oxidation takes place predominately in the liver and that the bladder plays, at most, a minor role in the formation of these presumed proximate urinary carcinogens.
D000588:Amines / Q000378:metabolism*; D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D002849:Chromatography, Gas; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008706:Methemoglobin / Q000096:biosynthesis; D008861:Microsomes / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D009092:Mucous Membrane / Q000378:metabolism / Q000648:ultrastructure; D010084:Oxidation-Reduction; D001743:Urinary Bladder / Q000378:metabolism* / Q000648:ultrastructure
D000588:Amines; D002273:Carcinogens; D008706:Methemoglobin