Zinc uptake in normal and leukemic lymphocytes: effect of poly-L-ornithine.
Phytohemagglutinin and poly-L-ornithine were tested for their ability to stimulate uptake of transferrin-bound zinc by lymphocytes from both normal donors and donors with chronic lymphocytic leukemia. The ratio of zinc uptake in poly-L-ornithine cultures to zinc uptake in phytohemagglutinin cultures was 0.9 +/- 0.3 for leukemic lymphocytes and 1.9 +/- 0.2 for normal lymphocytes. Results indicated that this technique may be useful in the diagnosis of chronic lymphocytic leukemia as well as in the assessment of the efficacy of chemotherapeutic regimens.
D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009952:Ornithine / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*; D014168:Transferrin / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
D037102:Lectins; D010455:Peptides; D013501:Surface-Active Agents; D014168:Transferrin; C008973:polyornithine; D009952:Ornithine; D015032:Zinc