Isolation of biologically active polysomal messenger ribonucleoprotein by centrifugation in cesium sulfate.
The ribosomal fraction from HeLa cells treated at elevated temperature was centrifuged in Cs2SO4. 1. The bulk of the RNA labeled in the presence of a low dose of actinomycin D banded at p = 1.36 g/cm3. 2. This fraction contained heterogeneous RNA and proteins with molecular weights close to those of proteins found in polysomal messenger ribonucleoproteins. 3. This ribonucleoprotein bound ribosomes in conditions were deproteinized RNA did not. 4. The binding was inhibited by aurintricarboxylic acid, ethylene diamine tetraacetic acid and sodium deoxycholate, but not by Triton X-100.
D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D002500:Centrifugation, Isopycnic; D003609:Dactinomycin / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology; D006367:HeLa Cells; D008970:Molecular Weight; D009698:Nucleoproteins / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013696:Temperature; D014529:Uridine / Q000378:metabolism
D009698:Nucleoproteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D003840:Deoxycholic Acid; D003609:Dactinomycin; D001312:Aurintricarboxylic Acid; D014529:Uridine