title: Immunoelectronmicroscopic localization of alpha-actinin in skeletal muscle cells.
abstract: The ultrastructural distribution of alpha-actinin in skeletal myocytes was studied by a sensitive method that permits localization of antigenic proteins in intact fixed cells rather than in glycerol estracted tissues or isolated cell fractions. Binding to the Z line of specific antiserum produced in rabbits immunized with highly purified alpha-actinin was demonstrated by deposition at this site of a reaction product of horseradish peroxidase that had been coupled to antigen-binding fragments of sheep anti-rabbit gamma globulin.
mesh_terms: D000185:Actinin / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D008854:Microscopy, Electron; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000032:analysis* / Q000648:ultrastructure
chemical_list: D009124:Muscle Proteins; D000185:Actinin