Direct evidence that ribosome bound RNA-dependent RNA polymerase does not play a role in globin messenger RNA replication.
The radioactively labelled product of RNA-dependent RNA polymerase+ from ribosomes of immature chicken erythrocytes was tested for the presence of newly replicated globin mRNA using unlabelled globin complementary DNA. No radioactively labelled globin mRNA sequences were found in the product, providing direct confirmation that this RNA-dependent RNA polymerase is not involved in globin mRNA amplification.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002645:Chickens; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005914:Globins / Q000096:biosynthesis*; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*; D012156:Reticulocytes / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
D012333:RNA, Messenger; D005914:Globins; D012321:DNA-Directed RNA Polymerases