High-performance liquid chromatography of pyruvic and alpha-ketoglutaric acids and its application to urine samples.
Pyruvic and alpha-ketoglutaric acids were quantitatively and rapidly converted to quinoxalones by a new method, to enhance their detectability in the ultraviolet. The quinoxalones were separated by reversed-phase chromatography with use of 10 micronm particles packed in a 30-cm (length) column. An aqueous solution of ammonium acetate and methanol was used for linear gradient elution. The urinary excretion of pyruvic and alpha-ketoglutaric acids by normal individuals, diabetic patients, and patients with renal dysfunction was determined.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007656:Ketoglutaric Acids / Q000652:urine*; D008722:Methods; D011773:Pyruvates / Q000652:urine*; D011810:Quinoxalines / Q000138:chemical synthesis / Q000652:urine
D007656:Ketoglutaric Acids; D011773:Pyruvates; D011810:Quinoxalines