[Immobilization of ferredoxin].
The method of pea ferredoxin (Fd) immobilization has been worked out. Under binding Fd with sepharose 4B activated by cyanogen bromide, a destruction took place in the Fd active center and apoprotein was bound with the matrix. The native immobilized Fd has been obtained when using P-300 biogel as a matrix. The gel was activated by glutaraldehyde and protein was linked with gel through a hexamethylenediamine spacer.
D001059:Apoproteins; D001665:Binding Sites; D003959:Diamines; D004800:Enzymes, Immobilized; D005288:Ferredoxins; D010944:Plants; D012685:Sepharose
D001059:Apoproteins; D003959:Diamines; D004800:Enzymes, Immobilized; D005288:Ferredoxins; D012685:Sepharose
Immobilizatsiia ferredoksina