The effect of fasting on the in vitro synthesis of amylase in rat exocrine pancreas.
Immunological methods were used to study the effect of fasting on the in vitro synthesis of amylase (EC 3.2.1.1) in rat exocrine pancreas. After 72 h of fasting, the amylase enzyme activity of the pancreas and the rate of amylase synthesis were reduced 50%. No significant change in the activities of trypsin or chymotrypsin were detected. The decrease in leucine incorporation in total pancreas protein was accounted for by the decreased amylase synthesis. No change in the rate of amylase breakdown was detected. These results indicate that the rate of synthesis of amylase is controlled by food intake and is not directly related to the tissue content of enzyme.
D000681:Amylases / Q000096:biosynthesis*; D000818:Animals; D002918:Chymotrypsin / Q000096:biosynthesis; D005260:Female; D010179:Pancreas / Q000201:enzymology* / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D013217:Starvation / Q000201:enzymology* / Q000378:metabolism; D014357:Trypsin / Q000096:biosynthesis
D011506:Proteins; D012313:RNA; D000681:Amylases; D002918:Chymotrypsin; D014357:Trypsin