title: Nucleolar proliferation and cell size changes in rat supraoptic neurons following osmotic and volemic challenges.
abstract: Subcutaneous injections of isotonic saline induced nucleolar proliferation in supraoptic neurons in animals sacrificed approximately 5 min postinjection. The magnitude of this proliferation was sustained 4 and 8 hr postinjection. Polyethylene glycol (PG) injections depleted blood volume 4 and 8 hr after the injection, but the percentage of SON cells with multiple nucleoli in these animals was not different from saline-injected controls. The anterior (SOa) portion of the SON in rats given 2% NaCl to drink instead of water for three days contained more cells with multiple nucleoli than controls. This effect was enhanced after five days ingestion, and accompanied by a similar response in the tuberal portion of SON (SOt). Rehydration for ten days after three days of 2% NaCl intake brought the percentage of cells with multiple nucleoli down to control levels. Cell area in SON cells paralleled nucleolar responses during dehydration and rehydration. The results demonstrate the sensitivity of nucleolar proliferation in SON to environmental changes ranging from osmotic to neurogenic stress.
mesh_terms: D000818:Animals; D001810:Blood Volume; D002466:Cell Nucleolus / Q000187:drug effects / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects; D009997:Osmotic Pressure; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D013495:Supraoptic Nucleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D011092:Polyethylene Glycols