Mechanism of "L"-type pyruvate kinase from rabbit liver. Evidence against phosphoenzyme formation.
The "L"-type pyruvate kinase from rabbit liver does not catalyse exchange between phosphoenol[1-14C]pyruvate and pyruvate at either pH 8.5 or 6.2. Spectrophotometric experiments at pH 8.5 and 6;2 and gel-filtration experiments with [32P]phosphoenolpyruvate at pH 8,5 also fail to demonstrate phosphoenzyme formation. It is concluded that it is very unlikely that the enzyme has a phosphoenzyme mechanism.
D000818:Animals; D007527:Isoenzymes / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology*; D010728:Phosphoenolpyruvate; D010750:Phosphoproteins; D011770:Pyruvate Kinase / Q000302:isolation & purification; D011817:Rabbits; D013053:Spectrophotometry
D007527:Isoenzymes; D010750:Phosphoproteins; D010728:Phosphoenolpyruvate; D011770:Pyruvate Kinase