Dog red blood cells: Na and K diffusion potentials with extracellular ATP.
External ATP causes a prompt increase in the Na and K permeability of dog red blood cells. By manipulating intra- and extracellular ion composition it is possible to observe ATP-induced net fluxes which can be explained in terms of the contribution of Na or K diffusion potentials to the membrane potential. Measurements of membrane voltage by a fluorescent dye technique confirm the existence of such potentials. A rough calculation of chloride permeability gives a value of the order of 10(-8) cm/s, which agrees with results in other species. The cells appear to be somewhat more permeable to bromide than to chloride.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001965:Bromides / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D004285:Dogs; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013997:Time Factors
D001965:Bromides; D002712:Chlorides; D000255:Adenosine Triphosphate; D012964:Sodium; D002794:Choline; D011188:Potassium