Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites.
Interspersed repetitive sequences were isolated from sea urchin DNA by renaturing to low Cot followed by treatment with nuclease SI. Synthetic Eco RI sites were ligated onto the repetitive sequence elements, which were then inserted at the Eco RI site of plasmid RSF2124 and cloned. The repetitive sequences can be excised from the plasmid with Eco RI for further study.
D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004274:DNA, Recombinant / Q000032:analysis*; D008970:Molecular Weight; D009695:Nucleic Acid Renaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D011118:Polynucleotide Ligases / Q000378:metabolism; D012617:Sea Urchins / Q000032:analysis*
D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes; D011118:Polynucleotide Ligases