[Multiple forms of 3-beta-hydroxy-steroid dehydrogenase of the adrenal glands of rats].
Experiments were conducted on Wistar rats. A study was made of the heterogeneity of 3beta-oxysteroiddehydrogenase (3beta-olDH) of the adrenal glands, the intracellular distribution of its multiple forms and the change of their activity in prolonged (for 3 months) keeping of the animals on a diet devoid of vitamin A. Four 3 beta-olDH fractions were revealed by the method of electrophoresis in polyacrylamide gel in the rat adrenal glands. The principal activity of 3 beta-olDH (68%) was revealed in the endoplasmic reticulum, and only 14%--in the mitochondrial-lysosomal fraction. Hypovitamonsis A caused a reduction of the 3 beta-olDH activity of the adrenal glands, chiefly on account of the 3 beta-olDH-2 fraction.
D000311:Adrenal Glands / Q000032:analysis / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D011375:Progesterone Reductase / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D014802:Vitamin A Deficiency / Q000378:metabolism
D006913:Hydroxysteroid Dehydrogenases; D011375:Progesterone Reductase
K voprosu o mnozhestvennykh formakh 3beta-oksisteroiddegidrogenazy nadpochechnikov krys