title: [DNA from chromatin-nuclear membrane complex: reassociation kinetics at different stages of the cell cycle].
abstract: Base composition and reassociation kinetics of DNA from chromatin-nuclear membrane complex of rat liver cells at different stages of the cell cycle. The complex isolated in low ionic strength solutions contains about 9% of total nuclear DNA, and its density in sucrose solution is 1.27 g/cm2. DNA, isolated from the complex, is homogenous in its molecular weight (approximately 2-10(6) daltons) and is similar in its base composition to total nuclear DNA. However, DNAs from complexes, isolated at interphase and different S-phases of the cell cycle, differ from total nuclear DNA in reassociation kinetics: DNAs from complexes are enriched by approximately 10% with unique nucleotide sequences. It is concluded from the similarity of reassociation kinetics, that molecular population of nuclear membrane-bound DNA does not change considerably throughout the cell cycle.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000032:analysis; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats
chemical_list: D002843:Chromatin; D003854:Deoxyribonucleotides; D004247:DNA
vernacular_title: DNK iz kompleksa khromatina s iadernoĭ obolochkoĭ: kinetika reassotsiatsii na raznykh stadiiakh kletochnogo tsikla