[Cytotoxic effect of lymphocytes on peritoneal macrophages of mice in contact dermatitis induced by dinitrochlorbenzene].
Contact dermatitis was induced in BALB/c mice by means of cutaneous application of dinitrochlorbenzol (DNCB) solution. Peritoneal macrophages from DNCB-sensitized and intact mice were cultivated in flat tubes for 48 hours after the 20th day of the experiment, when the DNCB cutaneous test became positive. A suspension of the peripheral lymph node lymphocytes was added to this culture. The lymphocytes fromthe DNCB-sensitized mice had a cytotoxic effect (CTE) on the target cells (peritoneal macrophages) during the co-incubation; the STE was tested with trypane blue in 48 hours. No macrophage lysis was observed in combined incubation of cells from the intact mice.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D003877:Dermatitis, Contact / Q000276:immunology*; D004137:Dinitrochlorobenzene; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009578:Nitrobenzenes
D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes
Tsitotoksicheskoe deĭstvie limfotsitov na peritoneal'nye makrofagi mysheĭ pri kontaktnom dermatite, vyzvannom dinitrokhlorbenzolom