Determination of dexamethasone in milk by high pressure liquid chromatography.
A rapid and sensitive high pressure liquid chromatographic method for the analysis of dexamethasone (9 alpha - fluoro - 11 beta, 17 alpha, 21 - trihydroxy - 16 alpha - methylpregna - 1,4-diene-3,20-dione) in milk has been developed. The corticosteroid can be quantitated at 20 ppb, with the limit of detectability estimated at 5 ppb. Recoveries ranged from 72 to 94%. After dexamethasone was injected into 5 Holstein-Friesian cows, no residues were dound in milk, even after the first milking.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003907:Dexamethasone / Q000032:analysis* / Q000378:metabolism; D005260:Female; D008722:Methods; D008892:Milk / Q000032:analysis*
D003907:Dexamethasone