Specific requirement of lysophosphatidylcholine for palmitoyl-CoA synthetase of chicken intestine.
The presence of palmitoyl-CoA synthetase (EC 6.2.1.3) in the brush border-free particulate fraction of chicken intestinal mucosa is demonstrated. The enzyme was dependent on the simultaneous presence of lysophosphatidylcholine and Triton X-100 as well as ATP, CoA and Mg2+ for maximal activity. Lysophosphatidylcholine could not be replaced by other lipids. Enzyme preparations solubilized by Triton X-100 or lysophosphatidylcholine were still dependent on the presence of detergents for maximal activity.
D000818:Animals; D002645:Chickens; D003066:Coenzyme A Ligases / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D010169:Palmitic Acids; D011092:Polyethylene Glycols / Q000494:pharmacology; D013347:Subcellular Fractions / Q000201:enzymology
D008244:Lysophosphatidylcholines; D010169:Palmitic Acids; D011092:Polyethylene Glycols; D003066:Coenzyme A Ligases; D008274:Magnesium