Ultrafiltration for improved assay of urinary enzymes.
Ultrafiltration of urine and rapidly separates urinary enzymes from inhibitors, activators, and heat-stable, spurious activities present in urine. Using eight enzymes of clinical interest as examples, we verified the reliability of results obtained after ultrafiltration by comparsion with results obtained after gel filtration.
D000116:Acetylgalactosamine; D000118:Acetylglucosaminidase / Q000652:urine; D000135:Acid Phosphatase / Q000652:urine; D000469:Alkaline Phosphatase / Q000652:urine; D002850:Chromatography, Gel; D004798:Enzymes / Q000652:urine*; D005696:Galactosidases / Q000652:urine; D005966:Glucuronidase / Q000652:urine; D006596:Hexosaminidases / Q000652:urine; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000652:urine; D007931:Leucyl Aminopeptidase / Q000652:urine; D014462:Ultrafiltration / Q000379:methods
D004798:Enzymes; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005696:Galactosidases; D006596:Hexosaminidases; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D007931:Leucyl Aminopeptidase; D000116:Acetylgalactosamine