The release of alanine by rat diaphragm muscle in vitro.
Alanine release by rat diaphragm muscle in vitro is stimulated by glutamate, valine, leucine and glucose. The stimulation by glutamate and valine (but not leucine) is inhibited by 3-mercaptopicolinate. These results suggest a metabolic route involving phosphoenolpyruvate carboxykinase which directs amino acid carbon skeletons towards pyruvate synthesis for alanine formation.
D000409:Alanine / Q000096:biosynthesis*; D000818:Animals; D003964:Diaphragm / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D051381:Rats; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D014633:Valine / Q000378:metabolism
D005971:Glutamates; D013438:Sulfhydryl Compounds; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D007930:Leucine; D014633:Valine; D005947:Glucose; D000409:Alanine