The clearance of lipoprotein X in vitro, and effect of phospholipase A on serum lipoprotein X.
The in vitro effect of post-heparin and post-heparin plus post-protamine normal serum on serum lipoprotein X (LP-X) is described. Serum LP-X is cleared after incubation with post-heparin normal serum, and serum LP-X remain unmodified when it is incubated with post-heparin plus post-protamine normal serum. The action of phospholipase A from snake venom on serum LP-X is also studied. A very small quantity of phospholipase is necessary to degrade LP-X and to transform lecithin into lysolecithin. It is concluded that phospholipase seems to be the enzyme that most likely induces the LP-X changes after heparin administration.
D000328:Adult; D001660:Biliary Tract Diseases / Q000097:blood; D002779:Cholestasis / Q000097:blood; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood*; D008244:Lysophosphatidylcholines / Q000097:blood; D008297:Male; D010713:Phosphatidylcholines / Q000097:blood; D010740:Phospholipases / Q000494:pharmacology*; D011479:Protamines / Q000494:pharmacology; D012910:Snake Venoms
D008055:Lipids; D008074:Lipoproteins; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D011479:Protamines; D012910:Snake Venoms; D006493:Heparin; D010740:Phospholipases