A new colorimetric method for determination of serum glucose.
A new colorimetric method using 4-aminoantipyrine-dimethylaniline in a glucose oxidase-peroxidase system is proposed to determine glucose in serum. The developed color was stable at the optimum pH of glucose oxidase, 5.6. The method is more sensitive than the o-dianisidine method and is reproducible. The correlation between the proposed method and the o-dianisidine method is 0.989.
D000814:Aniline Compounds; D000983:Antipyrine; D001786:Blood Glucose / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D005069:Evaluation Studies as Topic; D005949:Glucose Oxidase; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008832:Microchemistry; D010544:Peroxidases
D000814:Aniline Compounds; D001786:Blood Glucose; D007202:Indicators and Reagents; D005949:Glucose Oxidase; D010544:Peroxidases; D000983:Antipyrine