A simple enzymic method for the synthesis of adenosine 5'-[alpha-32P]triphosphate on a preparative scale.
A simple, rapid and inexpensive method is described for the enzymic synthesis of [alpha-32P]ATP from [32P]Pi on a preparative scale with an overall yield of 53%. The final product contained all of the detectable radioactivity (less than 99.9%) in the alpha position and has been shown to behave identically with commerically availabe [alpha-32P]ATP during the synthesis of 3':5'-cyclic AMP in the reaction catalysed by adenylate cyclase.
D000248:Adenosine Kinase; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000263:Adenylate Kinase; D007553:Isotope Labeling; D008722:Methods; D010761:Phosphorus Radioisotopes
D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate; D000248:Adenosine Kinase; D000263:Adenylate Kinase