Effect of graded biotin levels in the diet on liver pyruvate carboxylase of chicks fed ad libitum and after starvation.
Decreasing levels of biotin in the diet from 200 mug/kg to 0 mug/kg reduced the pyruvate carboxylase activity per g liver and in the whole liver of chicks to 17% of the normal activity. Oral supplementation with 3 mg biotin per chick increased the enzyme activity within 24 hours back to normal. In animals well supplemented with biotin (200 mug/kg diet) 24 hours of starvation increased the per g liver pyruvate carboxylase activity. When the pyruvate carboxylase activities were related to the whole liver, no effect of starvation was found.
D000818:Animals; D001710:Biotin / Q000008:administration & dosage / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002645:Chickens / Q000254:growth & development; D004032:Diet; D008099:Liver / Q000201:enzymology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011766:Pyruvate Carboxylase / Q000378:metabolism*; D013217:Starvation
D001710:Biotin; D011766:Pyruvate Carboxylase