title: Increased sensitivity of canine sarcoma cells to lysis with alloantiserum and complement after pretreatment of cells with doxorubicin HC1.
abstract: The 51Cr release assay was used to detect lysis of canine sarcoma cells incubated in vitro with alloantiserum and complement. Preincubation of the target cells with the anti-cancer agent doxorubicin-HCl increased the sensitivity of the cells to antibody-mediated lysis. The effect was dependent upon drug dose and was not reproduced by treatment with methotrexate, cytarabine, or dactinomycin. The observation suggests a possible ancillary mode of action for doxorubicin-HCl on neoplastic cells.
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D004285:Dogs; D004317:Doxorubicin / Q000494:pharmacology*; D007106:Immune Sera; D066298:In Vitro Techniques; D008297:Male; D012509:Sarcoma / Q000276:immunology*
chemical_list: D007106:Immune Sera; D004317:Doxorubicin; D003165:Complement System Proteins