title: Studies on the structure and possible function of the RNA "cap" in developing sea urchins.
abstract: Paracentrotus lividus embryos at the hatching blastula stage very quickly incorporate radioactivity from labeled nucleosides (except uridine) or 32P- or methyl-labeled methionine into a portion of the RNA that has been identified as a "cap". The most probable sequence of this cap is m7G (5') ppp (5')mAmpCp. A very active "capping" and methylation of the "cap" of preexisting RNA molecules was shown to occur at the blastula stage.
mesh_terms: D000818:Animals; D001483:Base Sequence; D003609:Dactinomycin / Q000494:pharmacology; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D008745:Methylation; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012617:Sea Urchins
chemical_list: D012333:RNA, Messenger; D003609:Dactinomycin