The effects of anti-inflammatory agents on skin tumor initiation and aryl hydrocarbon hydroxylase.
The effects of various clinically used anti-inflammatory agents on mouse skin tumorigenesis and aryl hydrocarbon hydroxylase (AHH) were investigated. Oxyphenbutazone, a nonsteroidal anti-inflammatory agent, inhibited 3-methylcholanthrene (MC) tumor initiation but was less effective than the steroidal anti-inflammatory agent, dexamethasone. Oxyphenbutazone was not found to induce AHH activity in mouse epidermis, whereas indomethacin and Seclazone had a slight inducing effect. When these agents were added directly to the in vitro AHH assay, they did not inhibit AHH activity. However, additional experiments have shown a decreased epidermally mediated covalent binding of MC to DNA in vitro when the epidermal homogenates were isolated from mice pretreated with either dexamethasone or oxyphenbutazone and MC at 3 or 12 hr before killing.
D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D002277:Carcinoma / Q000139:chemically induced / Q000517:prevention & control; D004247:DNA / Q000378:metabolism; D004347:Drug Interactions; D005260:Female; D066298:In Vitro Techniques; D008748:Methylcholanthrene / Q000378:metabolism; D051379:Mice; D010212:Papilloma / Q000139:chemically induced / Q000517:prevention & control; D012867:Skin / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D008748:Methylcholanthrene; D004247:DNA; D001189:Aryl Hydrocarbon Hydroxylases