Quantitative analysis of human serum cholesterol by thin-layer chromatographic spot test.
A fast, inexpensive, simple method is described for the determination of the total cholesterol concentration in human serum. The cholesterols (free and esterified) are extracted from serum by a double extraction procedure, using isopropanol-water-10 N sodium hydroxide (250:125:10) and then n-octane. An aliquot of the octane extract is spotted on a thin-layer plate and the cholesterol in the spot is rendered visible using an aqueous phosphomolybdic acid staining solution and a heat treatment for the color development. The colored spot is scanned with a densitometer.
D000433:1-Propanol; D000473:Alkanes; D002784:Cholesterol / Q000097:blood*; D002788:Cholesterol Esters / Q000097:blood; D002855:Chromatography, Thin Layer; D006801:Humans; D008722:Methods; D012997:Solvents
D000473:Alkanes; D002788:Cholesterol Esters; D012997:Solvents; D000433:1-Propanol; D002784:Cholesterol