title: Nucleotide sequence (n=159) of the amino-acid-accepting 3'-OH extremity of turnip-yellow-mosaic-virus RNA and the last portion of its coat-protein cistron.
abstract: The experiments described in this paper and the following one establish the sequence of the 3'-OH terminal 159 nucleotides of turnip yellow mosaic virus RNA. Uniformly 32P-labeled turnip yellow mosaic virus RNA was partially digested with T1 ribonuclease and the fragments were fractionated by polyacrylamide gel electrophoresis. Fragments originating from the 3'-OH end of the RNA molecule were identified by testing for the 3'-terminal oligonucleotide, C-COH, after total U2 ribonuclease hydrolysis. Once identified, the 3'-OH terminal fragments were sequenced by the methods of Sanger et al. The first 51 nucleotides of the longest of the sequenced fragments (158 nucleotides) extends into the 3'-terminal part of the coat protein cistron. The coat protein cistron is followed by a stretch of 108 untranslated nucleotides whose function, though still unknown, is probably linked to the tRNA-like properties which have been attributed to the 3'-OH extremity of this viral RNA. Two possible secondary structures are proposed for the sequence and the implications of the findings with regard to the tRNA-like properties of the extremity are discussed.
mesh_terms: D001483:Base Sequence; D008970:Molecular Weight; D009029:Mosaic Viruses / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D010942:Plant Viruses / Q000032:analysis*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism; D012260:Ribonucleases; D014764:Viral Proteins / Q000032:analysis*
chemical_list: D009843:Oligoribonucleotides; D012367:RNA, Viral; D014764:Viral Proteins; D012260:Ribonucleases