Role of ketone bodies in nonshivering thermogenesis in cold-acclimated rats.
The role of ketone bodies (KB) in nonshivering thermogenesis was investigated in warm- and cold-acclimated rats with and without norepinephrine (NE) loads. NE-induced calorigenesis, as evidenced by changes in rectal temperature, was highly developed in cold-acclimated rats, but the levels of blood KB and free fatty acids (FFA) remained almost unaltered. In contrast, FFA turnover rate in cold-acclimated rats under NE load was much greater than in warm-acclimated rats. Similarly, turnover rate of beta-hydroxybutyrate estimated from decay curves of the endogenous substrate in functionally eviscerated rats was significantly higher in cold- than warm-acclimated rats. Perfused livers from cold-acclimated rats produced more KB than warm-acclimated ones. No significant effect of NE load was observed in either group. Quantitative analysis shows that the turnover rate of KB in vivo essentially equals the production rate in the perfused liver when no exogenous NE is added. In contrast, under constant NE infusion the turnover rate in vivo was almost double that of the perfused liver. These results indicate that KB are an energy source as important as FFA in nonshivering thermogenesis. It may be further surmised that increased KB production in vivo, particularly in the cold-acclimated state, is affected by factor(s) other than NE.
D000064:Acclimatization; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D001833:Body Temperature Regulation; D003080:Cold Temperature; D004734:Energy Metabolism / Q000187:drug effects; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000378:metabolism*; D007657:Ketone Bodies / Q000097:blood / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D010168:Palmitates / Q000378:metabolism; D010477:Perfusion; D010646:Phentolamine / Q000494:pharmacology; D051381:Rats
D005230:Fatty Acids, Nonesterified; D007657:Ketone Bodies; D010168:Palmitates; D009638:Norepinephrine; D010646:Phentolamine