Temporal relationship between glucose 6-phosphate dehydrogenase activity and DNA-synthesis.
Wounds have been inflicted in the skin of guinea-pigs. Measurements were made on the basal cells 39-54 h later, at different times of the day. It has been shown that there can be two peaks of mitosis, one about mid-day and the other about 22 h. Synthesis of DNA, measured by Feulgen microdensitometry, preceded mitotic activity. Marked changes were found in glucose 6-phosphate dehydrogenase activity, measured by quantitative cytochemistry and microdensitometry. The greatest activity preceded DNA-synthesis, indicating that pentose-shunt metabolic activity is involved in biosynthetic processes required for cell proliferation.
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006168:Guinea Pigs; D008938:Mitosis; D010428:Pentosephosphates / Q000378:metabolism; D013997:Time Factors; D014945:Wound Healing
D010428:Pentosephosphates; D004247:DNA; D005954:Glucosephosphate Dehydrogenase