Prostaglandin E in the secretions of allergic rhinitis.
Prostaglandin (PG) was extracted from nasal secretions of individuals with hay fever and from nasal washings of normal subjects. The extract was chromatographed in a silicic acid column and the purified PGE fraction was converted to PGB by alkaline dehydration. The PGB was then measured by a competitive radioimmunoassay with tritiated PGB1 and anti-PGB1 antibody, employing the double antibody technique. PGE was detected in the secretions of 6 of 12 hay fever patients and in the pooled normal nasal washings.
D000293:Adolescent; D000328:Adult; D001667:Binding, Competitive; D002648:Child; D002675:Child, Preschool; D004804:Eosinophils; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D007958:Leukocyte Count; D008297:Male; D009297:Nasal Mucosa / Q000032:analysis* / Q000378:metabolism; D011458:Prostaglandins E / Q000032:analysis / Q000302:isolation & purification; D011863:Radioimmunoassay / Q000379:methods; D011864:Radioimmunosorbent Test; D006255:Rhinitis, Allergic, Seasonal / Q000378:metabolism*
D011458:Prostaglandins E; D007073:Immunoglobulin E