The effect of S-adenosylhomocysteine on DNA methylation in isolated rat liver nuclei.
DNA methylation was studied in vitro using whole nuclei from regenerating rat liver. Methyl incorporation from S-adenosyl-[Me-3H]methionine in nuclei from regenerating liver was four times higher than that of normal liver. The effect of S-adenosylhomocysteine on DNA methylation was examined, and it was found at equal molar concentrations of S-adenosylhomocysteine to to S-adenosylmethionine that DNA methylation was competitively inhibited 50%.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006710:Homocysteine / Q000031:analogs & derivatives*; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D008745:Methylation; D051381:Rats; D012435:S-Adenosylhomocysteine / Q000494:pharmacology*
D006710:Homocysteine; D004247:DNA; D012435:S-Adenosylhomocysteine