Chemical synthesis of restriction enzyme recognition sites useful for cloning.
By a triester chemical synthesis method, three decameric DNA's have been made; these act as substrates for several restriction endonucleases, including Eco RI, Bam I, and Hind III. These homogenous decamers form duplexes that can be efficiently blunt-end ligated to themselves or to other DNA molecules by the action of T4 DNA ligase and thus are useful tools for molecular cloning experiments.
D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D004274:DNA, Recombinant / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010957:Plasmids; D011118:Polynucleotide Ligases / Q000378:metabolism
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D011118:Polynucleotide Ligases