A single-strand-specific DNA-binding protein from mouse cells that stimulates DNA polymerase.
A protein of 30000-35000 molecular weight was isolated from mouse ascites cells. This protein binds preferentially to single-stranded DNA. Evidence is presented that this protein maintains single-stranded DNA in an extended configuration. In the presence of single-stranded template the protein stimulates mammalian DNA polymerase alpha but not the mammalian DNA polymerase beta and not some microbial DNA polymerases. The protein is phosphorylated in vitro by a chromatin-associated protein kinase. The modified DNA-binding protein does not stimulate the DNA polymerase alpha.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D002352:Carrier Proteins / Q000302:isolation & purification / Q000502:physiology*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004789:Enzyme Activation; D007700:Kinetics; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism*; D012965:Sodium Chloride
D002352:Carrier Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D010750:Phosphoproteins; D012965:Sodium Chloride; D004259:DNA-Directed DNA Polymerase