CS2 binding to rat spinal neurofilaments.
The binding of CS2 sulphur to rat spinal cord neurofilaments was studied 3 and 6 h after the intraperitoneal injection of 1.3 mmol of CS2 in control and phenobarbitone pretreated rats. The binding of CS2 carbon was studied similarly 3 h after the injection. The binding of CS2 sulphur was 31% higher in the control neurofilaments than that in the phenobarbitone pretreated group. Half of the neurofilament associated CS2 sulphur was bound to protein in both groups as studied by electrophoresis. Approximately 30% of the neurofilament associated CS2 sulphur was detected 6 h after the injection of that at 3 h in both groups. All of the neurofilament associated CS2 carbon was bound to protein in control group 3 h after the injection. The  binding of CS2 carbon was 4.7 times less than that of sulphur at the same time. The present data may indicate that the high binding of CS2 to the neurofilament protein contributes to the increase in neurofilaments in chronic CS2 intoxication.
D000818:Animals; D002246:Carbon Disulfide / Q000633:toxicity*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009454:Neurofibrils / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D009419:Nerve Tissue Proteins; D002246:Carbon Disulfide; D010634:Phenobarbital