A simple radioimmunological method for the determination or renal renin activity in the rat.
A RIA method for the measurement of RRA is described, based upon the incubation of two dilutions of kidney extract with a fixed amount of EDTA-anticoagulated anephric rat plasma. The RIA of the generated angiotensin I is carried out with the commercially available NEN Kit. Recovery  of angiotensin I added to the incubation mixture was 98 percent. There were 113 kidneys with a wide range of renin activity (52 to 1,274 ng. angiotensinl/mg. of kidney per hour) assayed. The within-assay coefficient of variation was uniform throughout the range, equalling 4.4 percent. The between-assay variance was practically identical with the within-assay variance. The confidence interval to the mean RRA level of one pair of readings was 5.4 percent of this mean.
D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001769:Blood; D007668:Kidney / Q000032:analysis*; D009392:Nephrectomy; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D012083:Renin / Q000032:analysis*
D000804:Angiotensin II; D012083:Renin