[Antigenic composition of protein fractions of the human cerebral cortex extracted by chromatography on DEAE-cellulose].
In fractionating a phosphate protein extract from the human brain cortex on DEAE-cellulose 10 fractions of the basic acid proteins were received. Analyses of these fractions were conducted by the method of disc electrophoresis in polyacridamide gel with dodecylsulphate Na and a subsequent separate staining of the desitograms of proteins and glycoproteins, precipitation in agar gel, immunoelectrophoresis and autoimmunography with the use of labeled I125 antibodies to rabbit gamma-globulin. In the first 2 basic fractions there were brain specific antigens with electrophoretic mobility of the beta2--gamma globulin. In the first acid fraction--2 antigens: alpha1-globulin and alpha2-globulin of a glycoprotein nature. In subsequent acid fractions--one antigen with a mobility of alpha1-globulin. A study of the antigen activity of these fractions in a complement fixation test on cold with the serum of schizophrenic patients and disseminated sclerosis depicted differences in the activity of the basic and acid fractions.
D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000302:isolation & purification*; D002540:Cerebral Cortex / Q000032:analysis / Q000276:immunology*; D002848:Chromatography, DEAE-Cellulose; D003080:Cold Temperature; D003168:Complement Fixation Tests; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007122:Immunoelectrophoresis; D009419:Nerve Tissue Proteins / Q000276:immunology* / Q000302:isolation & purification; D009928:Organ Specificity; D012559:Schizophrenia / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D006023:Glycoproteins; D009419:Nerve Tissue Proteins
Antigennyĭ sostav belkovykh fraktsiĭ kory golovnogo mozga cheloveka, poluchennykh khromatografieĭ na DEAE-tselliuloze