Protein binding of rifampicin to bovine serum albumin as measured by gel filtration.
A "large zone-small zone elution method" with Sephadex G-25 has been developed for the study of the interactions of [14C]rifampicin with bovine serum proteins. The method was demonstrated to be capable of yielding both qualitative and quantitative information about the binding reactions. However, its application requires a large amount of experimental work, and therefore it should be reserved for instances when other procedures are not applicable, e.g., with highly lipophilic ligands.
D002850:Chromatography, Gel; D008433:Mathematics; D011485:Protein Binding; D012293:Rifampin; D012710:Serum Albumin, Bovine; D013696:Temperature
D012710:Serum Albumin, Bovine; D012293:Rifampin