Evidence for the preferential interaction of glycophorin with negatively charged phospholipids.
Glycophorin, extracted from the erythrocyte membrane after treatment with lithium-diiodo-salicylate, still contains a significant amount of phospholipid, consisting predominantly of phosphatidylserine. Methods are described which lead to a full delipidation of the protein. After treatment with neuraminidase, delipidated glycophorin shows a preferential interaction with monolayers of negatively-charged phospholipids. This lipid-protein interaction is decreased by the presence of cholesterol in the lipid film.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002784:Cholesterol / Q000032:analysis; D004910:Erythrocyte Membrane / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure*; D006021:Glycophorins / Q000302:isolation & purification; D006801:Humans; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D011312:Pressure; D012794:Sialic Acids / Q000032:analysis; D012795:Sialoglycoproteins / Q000302:isolation & purification; D013499:Surface Properties
D006021:Glycophorins; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012794:Sialic Acids; D012795:Sialoglycoproteins; D002784:Cholesterol