title: Wall-less microbial isolate from a human renal biopsy.
abstract: An orgainsm was isolated from a kidney biopsy of a patient with renal failure. Electron microscopy revealed an ultrastructure very similar to that of a bacterial L-form or mycoplasma. But macroscopically, its colonial morphology was unusual and distinct from that ascribed to these wall-less organisms. This isolate lacked a rigid cell wall and required a hypertonic medium with serum for growth. Also, a long incubation period was essential for its growth, and use of hand lens was necessary for detection on solid medium.
mesh_terms: D001707:Biopsy, Needle; D002473:Cell Wall / Q000648:ultrastructure; D006801:Humans; D007668:Kidney / Q000382:microbiology* / Q000601:surgery; D007740:L Forms / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged