[Isolation and characterization of a retinal antigen inducing experimental autoimmune uveo-retinitis].
A soluble antigen was extracted from the retinas of different mammals and purified by isoelectric focusing in a sucrose density gradient-ampholine column (LKB, Sweden). The isoelectic point of the molecule is about 5.5, its molecular weight about 55,000 and its electrophoretic mobility with reference to albumin 0.7. The S antigen is revealed by one precipitin line in immunodiffusion and immunoelectrophoresis with guinea pig immune sera against bovine or guinea pig retinas, and shows an identity reaction with S antigen from other species. The antigen, inoculated with complete Freund's adjuvant into the guinea pig foot pad, induces experimental uveo-retinitis.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D001327:Autoimmune Diseases / Q000150:complications; D006168:Guinea Pigs; D012160:Retina / Q000276:immunology*; D012173:Retinitis / Q000150:complications / Q000276:immunology*
D000941:Antigens
Isolement et caractérisation partielle d'un antigène rtinien responsable de l'uvéo-rétinite autoimmune expérimentale