Development of a chemically defined medium for the synthesis of actinomycin D by Streptomyces parvulus.
A chemically defined medium, consisting of d-fructose, l-glutamic acid, l-histidine, K(2)HPO(4), MgSO(4).7H(2)O, ZnSO(4).7H(2)O, CaCl(2).2H(2)O, FeSO(4).7H(2)O, CoCl(2).6H(2)O, and deionized water, was developed for synthesis of high yields (500 to 600 mug/ml) of actinomycin D by Streptomyces parvulus. Under these nutritional conditions, growth and actinomycin formation did not follow a typical trophophase-idiophase pattern. The amino acids appeared to have a sparing action on the utilization of d-fructose which was slowly and incompletely metabolized during mycelium development and antibiotic production. A significant repression of actinomycin synthesis by S. parvulus was observed when d-glucose (0.01 to 0.25%) was added to the culture medium. The repression was not due to a decline in the pH of the medium during glucose catabolism.
D000596:Amino Acids / Q000494:pharmacology; D050260:Carbohydrate Metabolism; D003470:Culture Media; D003609:Dactinomycin / Q000096:biosynthesis*; D005632:Fructose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D008670:Metals / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D013302:Streptomyces / Q000378:metabolism*; D013997:Time Factors
D000596:Amino Acids; D003470:Culture Media; D008670:Metals; D010710:Phosphates; D003609:Dactinomycin; D005632:Fructose; D005947:Glucose; D009584:Nitrogen