A tryptic digestion fragment of nerve growth factor with nerve growth promoting activity.
A peptide, isolated from the acid-insoluble portion of a tryptic digest of cyanogen bromide cleaved nerve growth factor, favors life maintenance of sensory target cells and promotes rapid neurite outgrowth from 7-day-old chick embryo sensory ganglia. The fragment has been identified as a 30 amino acid peptide consisting of two linear oligopeptides linked by a disulphide bridge and corresponding to residues 10-25 and 75-88 of the amino acid sequence of nerve growth factor. On a molar basis the fragment is about 100 times more effective than intact nerve growth factor. Other peptides isolated from the digest are biologically inactive.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002642:Chick Embryo; D003488:Cyanogen Bromide; D005724:Ganglia / Q000254:growth & development; D008297:Male; D051379:Mice; D009414:Nerve Growth Factors / Q000302:isolation & purification / Q000494:pharmacology*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology*; D014357:Trypsin
D000596:Amino Acids; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D014357:Trypsin; D003488:Cyanogen Bromide