Immunochemical studies on a nephritis-associated ubiquitous tissue antigen.
A ubiquitous tissue antigen (UTA), associated with chronic renal diseases was partially purified and characterized. The antigen was extracted by solubilization in 0.5% sodium deoxycholate (DOC) of various organs of human and animal origin. UTA was soluble in ammonium sulfate at 50% concentration and precipitable in 71% ethanol. Considerable purification of UTA was achieved by fractional ammunium sulfate precipitation, zone electrophoresis and gel filtration. Gel filtration on Sephadex G-200 and velocity gradient centrifugation through 10-40% sucrose gradients suggested that the main antigenic component is a macromolecule, although aggregability of DOC-extracted proteins upon removal of the solubilizer prevented more accurate determinations. UTA appeared to be a heat-stable glycoprotein which did not contain lipids detectable by Sudan black B staining. The yield of purest UTA preparations, which did not contain components of homologous serum as tested by double diffusion gel precipitation or immunoelectrophoresis, was 0.1-0.2 mg/100 g of starting tissue. Immunization of rabbits with homologous UTA-containing preparations resulted in anti-UTA antibody formation.
D000645:Ammonium Sulfate; D000917:Antibody Formation; D000941:Antigens / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003168:Complement Fixation Tests; D003840:Deoxycholic Acid; D000431:Ethanol; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D009393:Nephritis / Q000276:immunology*; D012995:Solubility
D000941:Antigens; D006023:Glycoproteins; D003840:Deoxycholic Acid; D000431:Ethanol; D000645:Ammonium Sulfate