The relationship between growth, developmental stage and postamputation age of the regeneration blasterma of the newt, Notophthalmus viridescens.
The growth of the regeneration blastema of the newt forelimb has been quantitated and analyzed as a function of postamputation age, developmental stage, animal weight, animal length, and cross sectional diameter of the blastemal base in both the anteroposterior and dorsoventral dimensions. Correlation coefficients computed for these variables show that growth of the regenerate in both length and volume is more closely correlated with developmental stage than postamputation age. In addition, the results show a linear relation between the loge (regenerate length) and developmental stage, and between regenerate length and volume. Thus, length can be used to assess growth of the regenerate according to a developmental rather thana chronological time scale. There were no significant correlations between regenerate length or volume and animal length, animal weight or cross sectional dimensions of the blastemal base. These results show that one can use a randomly selected population of animals and study the growth of the regeneration blastema by relating to a developmental time scale through a logarithmic transformation of the linear growth data.
D000671:Amputation, Surgical; D000818:Animals; D002454:Cell Differentiation; D005552:Forelimb / Q000502:physiology; D012038:Regeneration; D012455:Salamandridae