[Macromolecular biochemistry of normal and pathological white blood cells in man].
Leucocytes from normal donors and leukemia patients were isolated and lebelled in vitro with 32P-orthophosphate in order to compare labelling characteristics of nuclear high-molecular weight RNA, labelling characteristics, nucleotide compositions and oligonucleotide frequencies of ribosomal 28 S RNA. These studies revealed 1. structural microheterogeneity of 28 S RNA between the various leukemia cells studies without presenting a leukemia-specific structural marker, 2. an impaired production of ribosomal 28 S RNA from its nuclear precursor 45 S RNA in acute myeloblastic leukemia compared to PHA-stimulated normal lymphocytes. In the second part of this work, the influence of RNA from immunocompetent lymphocytes on the PHA-stimulation of M. Hodgkin lymphocytes was analyzed; the third part deals with studies on macromolecular carriers forcytostatic anthracyclines in human leukemia cells.
D006689:Hodgkin Disease / Q000032:analysis / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D007553:Isotope Labeling; D007938:Leukemia / Q000032:analysis* / Q000473:pathology; D007962:Leukocytes / Q000032:analysis*; D046911:Macromolecular Substances / Q000032:analysis; D010761:Phosphorus Radioisotopes; D012334:RNA, Neoplasm / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis
D046911:Macromolecular Substances; D010761:Phosphorus Radioisotopes; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal
Untersuchungen zur makromolekularen Biochemie normaler und pathologischer weisser Blutzellen des Menschen