Interactions between selenium and methylmercury in rat brain.
The interaction of selenium with methylmercury was investigated in brain of animals labeled with 75SeO2-3 and CH3203Hg+. Brains were fractionated into subcellular components and the cytosol was further fractionated by chromatography on Sephadex G-150 and G-200. The main result of these studies was evidence suggesting a shift of 75Se from the cytosol to the mitochondrial fraction in brain when CH3Hg+ was given. Concurrent equmolar (10 micronmoles/kg) selenite injections increased the uptake of Hg but did not alter 203Hg distribution in brain. Changing the dose of CH3Hg+ from 1 to 38 micronmoles/kg had little effect of Hg uptake (% of dose per g). Gel filtrations on Sephadex G-150 and G-200 revealed that 203Hg in cytosol followed a pattern more closely related to protein (A280) than to 75Se, although a considerable portion of both isotopes eluted with proteins in the void vulume. Assays of whole brain homogenates revealed a slight reduction in glutathione peroxidase activity in CH3Hg+-treated rats which was not seen when equimolar selenite was injected with the CH3Hg+
D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008628:Mercury / Q000097:blood / Q000378:metabolism; D008767:Methylmercury Compounds / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism; D051381:Rats; D012643:Selenium / Q000097:blood / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism
D008767:Methylmercury Compounds; D005979:Glutathione Peroxidase; D008628:Mercury; D012643:Selenium