Interaction of difluoro-oxaloacetate with aspartate transaminase.
Diffluoro-oxaloacetate behaves as a competitive inhibitor of 2-oxoglutarate and as an uncompetitive inhibitor with respect to aspartate in steady-state kinetic experiments with cytoplasmic aspartate transaminase. In the presence of high concentrations of aspartate transaminase, difluoro-oxaloacetate is slowly transaminated to difluoro-aspartate, suggesting its use as a kinetic probe to study the reactions of the aminic form of the enzyme.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000037:antagonists & inhibitors; D007700:Kinetics; D010071:Oxaloacetates / Q000494:pharmacology; D013552:Swine
D010071:Oxaloacetates; D001219:Aspartate Aminotransferases