Photoinduced size change of membrane vesicles from bovine retinal rod outer segment.
Quasielastic light-scattering measurements were made on isolated, osmotically swollen, bovine rod outer segment (ROS) disk membranes in aqueous Ficoll. The translational diffusion coefficients determined were (1.66 +/- 0.21) X 10(-9) and (2.31 +/- 0.14) X 10(-9) cm2/s at 25 degrees C for the unbleached and bleached membranes in 5% Ficoll, respectively, yielding the Stokes radii of 0.53 +/- 0.07 micronm for the former and 0.38 +/- 0.02 micronm for the latter. This photoinduced size decrease, which corresponds to a 60% decrease in the intravesicular volume, is far more pronounced than thos reported by others for ROS fragments.
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D007700:Kinetics; D008027:Light; D008433:Mathematics; D010786:Photoreceptor Cells / Q000502:physiology* / Q000648:ultrastructure; D012542:Scattering, Radiation; D013057:Spectrum Analysis