Messenger RNA for myosin polypeptides: isolation from single myogenic cell cultures.
Messenger RNA which stimilates the synthesis of myosin heavy chain in a reticulocyte lysate has been isolated from single myogenic cell cultures. Specific myosin polypeptides have been identified by immunoprecipitation with an antibody made to purified adult chicken skeletal muscle myosin. This mRNA binds to oligo(dT)-cellulose, and an active fraction from sucrose gradients migrates as 26S on formamide-polyacrylamide gels. The relative amount of this RNA increases dramatically at the time of terminal differentiation.
D002454:Cell Differentiation; D002455:Cell Division; D002459:Cell Fusion; D002478:Cells, Cultured; D009132:Muscles / Q000378:metabolism*; D009218:Myosins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism
D012333:RNA, Messenger; D009218:Myosins