[Properties of mitochondrial monoamine oxidase isolated from rabbit brain and liver].
The effect of pO2 on the monoamine oxidase activity of mitochondria from rabbit brain and liver was investigated using the substrates tyramine, dopamine, tryptamine and serotonin. The effect of the second substrate (oxygen) was dependent upon the concentration of the first substrate (the amine). At amine concentrations below 50 micronM, the reaction rate as measured by a radiometric assay, was not affected by variations in the pO2. It was found that both phenazine methosulfate (PMS) and chlorpromazine (CPZ) are reversible inhibitors of monoamine oxidase, the former was a potent inhibitor (Ki=3X10(-6) M) and the latter relatively weak (Ki=5X10(-4) M). Inhibition by both compounds was non-competitive with respect to the amine substrate. Imipramine was a weak inhibitor of purified MAO from beef kidney and of the MAO activity of mitochrondria from brain and liver. Using tyramine or dopamine as the substrate (0.5-1.0 mM), inhibition ranging from 6-30% was observed at 5X10(-4) M imipramine. With tryptamine or serotonin (0.5-1.0 mM) as the substrate in the presence of 5X10(-4) M imipramine the drug seemed to have no net effect on MAO activity since the average value in the presence of imipramine for a number of experiences was the same as the average for control experiments. For p-iodo-phenyl-3-p-nitrophenyl tetrazolium chloride, a Ki of 43X10(-6) M was found using dopamine as the substrate and oxygen as the gas phase.
D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000648:ultrastructure; D002746:Chlorpromazine / Q000494:pharmacology; D007099:Imipramine / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D008773:Methylphenazonium Methosulfate / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D011817:Rabbits; D013778:Tetrazolium Salts / Q000494:pharmacology
D013778:Tetrazolium Salts; D008773:Methylphenazonium Methosulfate; D008995:Monoamine Oxidase; D007099:Imipramine; D002746:Chlorpromazine
Svoĭstva monoaminooksidazy mitokhondriĭ, izolirovannykh iz mozga i pecheni krolika