Improved assay for monocyte chemotaxis using frozen stored responder cells.
Frozen stored human mononuclear cells were compared with non-frozen cells as responder cells in the monocyte chemotaxis assay. Frozen stored cells were found to inhibit lower background migration, resulting in greater experimental-to-control ratios. The use of frozen cells also greatly improved the speed, standardization of conditions and day-to-day reproducibility of the assay procedure. Frozen cells were at least as sensitive as fresh cells for detecting low chemotactic factor concentrations and were superior for detecting differences in concentration.
D001793:Blood Preservation; D002462:Cell Membrane; D002634:Chemotaxis, Leukocyte; D005615:Freezing; D006801:Humans; D009000:Monocytes; D013997:Time Factors