title: Isoproterenol stimulation of heme synthesis in cultures of human fetal liver from early gestation.
abstract: The role of isoproterenol in the synthesis of heme associated with hemoglobin has been studied in primary cultures of human fetal liver. This drug (10-10M) stimulated the incorporation of 59Fe into heme associated with hemoglobin in cell cultures of livers obtained from human fetuses 8 to 10 weeks gestation but was inactive in cell cultures prepared from livers of older fetuses. Isoproterenol has its optimal activity at a much earlier time in gestation than that previously reported for testosterone or erythropoietin. The interpretation of these results is that there is present a cell population in human fetal liver early in gestation with the appropriate isoproterenol-receptors and capable of erythroid differentiation after exposure to the drug.
mesh_terms: D046508:Culture Techniques; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D005865:Gestational Age; D006418:Heme / Q000096:biosynthesis*; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D011247:Pregnancy; D013739:Testosterone / Q000494:pharmacology
chemical_list: D006454:Hemoglobins; D004921:Erythropoietin; D013739:Testosterone; D006418:Heme; D007545:Isoproterenol