title: Host cell and ultraviolet reactivation of ultraviolet-irradiated Mycoplasmaviruses.
abstract: The mycoplasma Acholeplasma laidlawii was shown to have mechanisms for both host cell and ultraviolet (UV) reactivation of UV-irradiated mycoplasmaviruses. Host cell reactivation was examined by comparing the survival abilities of UV-irradiated double-stranded deoxyribonucleic acid mycoplasmavirus plated on both untreated and on acriflavine-treated cells. Acriflavine treatment inhibited cell exision repair. Decreased survival on the acriflavine-treated cells demonstrated host cell reactivation. UV reactivation was studied by comparing the survival of UV-irradiated virus plated on untreated cells with its survival on cells that received a small UV dose before plating. The UV-irradiated cells gave increased virus survival, showing UV reactivation. Similar experiments with a single-stranded deoxyribonucleic acid mycoplasmavirus showed that this virus could be UV reactivated, but not host cell reactivated.
mesh_terms: D000128:Acholeplasma laidlawii / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D000167:Acriflavine / Q000494:pharmacology; D001435:Bacteriophages / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects*; D004260:DNA Repair; D014466:Ultraviolet Rays
chemical_list: D000167:Acriflavine