Effect of thyrotropin on ornithine decarboxylase and of polyamines on RNA polymerase in the thyroid.
Two major forms of RNA polymerase were partially purified from calf thyroid nuclei. Type I showed greatest activity in 30 mM(NH4)2SO4, transcribed native DNA best and was resistant to the inhibitor alpha-amanitin. Type II showed greatest activity in 100 mM(NH4)2SO4, transcribed denatured DNA template best and was sensitive to low concentrations of alpha-amanitin...
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002417:Cattle; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004285:Dogs; D066298:In Vitro Techniques; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011073:Polyamines / Q000494:pharmacology*; D011700:Putrescine / Q000494:pharmacology; D012318:RNA Polymerase I / Q000302:isolation & purification / Q000378:metabolism; D012319:RNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D013961:Thyroid Gland / Q000187:drug effects / Q000201:enzymology*; D013972:Thyrotropin / Q000494:pharmacology*
D011073:Polyamines; D013096:Spermine; D013972:Thyrotropin; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D013095:Spermidine; D011700:Putrescine