[Inaccuracy of serum albumin measurements in renal failure patients using a dye-binding method (author's transl)].
A survey of serum albumin determinations in a group of patients with renal failure revealed that albumin reasurements using the 2-(4'-hydroxyphenylazo)-benzoic acid (HABA) dye-binding method were understimated when compared to results obtained with the biuret method. Equilibrium dialysis of the HABA dye binding to albumin are reported. Scatchard plot analysis showed that an average of 5.7 binding sits per molecule of albumin were unavailable in renal failure patients. The binding interference encountered in these patients indicates that alubumin measurements using dye binding techniques should not be used for these patients.
D001391:Azo Compounds; D003956:Dialysis; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D008722:Methods; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis*
D001391:Azo Compounds; D012709:Serum Albumin
Insuffisance renale et interference dans le dosage de l'albumine serique par une methode de liaison de colorant