The phosphorylation sites of troponin T from white skeletal muscle and the effects of interaction with troponin C on their phosphorylation by phosphorylase kinase.
1. The phosphorylation of troponin T from rabbit white sketetal muscle is catalysed by phosphorylase kinase, but not at a significant rate by bovine 3':5'-cyclic AMP-dependent protein kinase. 2. The amino acid sequences adjacent to the three major phosphorylation sites of troponin T were determined. 3. The serine in the N-terminal peptide (Asx,SerP, Glx)Glu-Val-Glu, is that phosphorylated (SerP, phosphoserine) when the troponin complex is isolated. 4. The other two sites of phosphorylation are located in the sequence Ala-Leu-(Ser, SerP)-Met-Gly-Ala-Asn-Tyr(Ser,SerP)Tyr. 5. When troponin T is phosphorylated in the presence of troponin C, the extent of phosphorylation at each site is considerably decreased. 6. CNBr fragments of troponin T are also phosphorylated by phosphorylase kinase, but the rate of phosphorylation at each site in the CNBr fragments is considerably slower than in the native protein. 7. From these studies it is suggested that troponin C interacts with troponin T in the region containing the two closely situated phosphorylation sites.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D004590:Electrophoresis, Paper; D007700:Kinetics; D009124:Muscle Proteins / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D010764:Phosphorylase Kinase / Q000378:metabolism*; D011817:Rabbits; D014336:Troponin / Q000032:analysis / Q000378:metabolism*; D014357:Trypsin
D000596:Amino Acids; D009124:Muscle Proteins; D010446:Peptide Fragments; D010710:Phosphates; D014336:Troponin; D010764:Phosphorylase Kinase; D014357:Trypsin