title: No direct activation of fluid phase C3 by lymphokines.
abstract: Supernatants of stimulated (PPD or Con A) or unstimulated guinea pig lymph node cell cultures in serum-free medium were fractionated on Sephadex G 75. The fractions were tested for C3-consuming activities in a hemolytical assay. No C3 consumption could be detected in any fraction (MW range 80,000 to 10,000). The same result was obtained by extending the fractionation range using Sephadex G 200. A lytic activity with a molecular weight of appr. 225,000 which lysed EAC 14 cells in the presence of C2, 5, 6, 7, 8, 9 was attributed to serum C3 contaminating the cultures. It was determined that C3 is not produced in significant amounts by lymphocyte cultures.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003176:Complement C3 / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism*; D003208:Concanavalin A; D006168:Guinea Pigs; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000502:physiology*; D014373:Tuberculin
chemical_list: D003176:Complement C3; D008222:Lymphokines; D014373:Tuberculin; D003208:Concanavalin A; D003165:Complement System Proteins