Liposome-cell interaction: transfer and intracellular release of a trapped fluorescent marker.
When small, unilamellar lipid vesicles containing a high concentration of the fluorescent dye 6-carboxyfluorescein are incubated with either frog retinas or human lymphocytes, fluroescence distributes widely throughout each cell. Since "self-quenching" largely prevents the dye from fluorescing as long as it remains sequestered in vesicles, it is clear that a considerable amount of dye is released from the vesicles and diluted into the much larger volume of the cell.
D000818:Animals; D002469:Cell Separation / Q000295:instrumentation; D005452:Fluoresceins / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008081:Liposomes; D008214:Lymphocytes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D010713:Phosphatidylcholines; D012160:Retina / Q000378:metabolism*
D005452:Fluoresceins; D008081:Liposomes; D010713:Phosphatidylcholines