Purification of UDP-glucose dehydrogenase by hydrophobic and affinity chromatography.
UDP-glucose dehydrogenase was purified from an extract of calf liver acetone powder by sequential chromatography on norleucine-agarose, Type 2, agarose-hexane-AMP, and UDP-hexanolamine-Sepharose. The overall yield of the highly purified enzyme was 80% and the subunit molecular weight was 52,000.
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000302:isolation & purification*
D000429:Alcohol Oxidoreductases; D014533:Uridine Diphosphate Glucose Dehydrogenase