Protein biosynthesis in vitro by the pars intermedia of the mouse pituitary gland.
During short-term incubations of isolated posterior pituitary glands of the mouse, isotopically labelled amino acids were incorporated into protein by the cells of the pars intermedia. Using labelled leucine, 5-10% of incorporated label was found in a protein (P1) with a molecular weight of about 75 000. Protein P1 could be isoalted from both fresh and incubated tissue, and was a normal and indeed major, secretory product of the ppars intermedia. constituting more than 50% of the protein present.
D000818:Animals; D004588:Electrophoresis, Cellulose Acetate; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight; D010904:Pituitary Gland, Posterior / Q000378:metabolism*; D014176:Protein Biosynthesis