title: The turnover of hamster fibroblast lysosomal beta-D-glucuronidase.
abstract: The half-life of hamster fibroblast beta-D-glucuronidase (EC 3.2.1.31) was estimated to be 4-5 days by measuring the decay with time of the radioactivity in beta-D-glucuronidase isolated from cells grown in the presence of 14C-labelled amino acids. A new affinity-chromatographic procedure for the purification of beta-D-glucuronidase is described.
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D006224:Cricetinae; D005347:Fibroblasts / Q000201:enzymology*; D005966:Glucuronidase / Q000302:isolation & purification / Q000378:metabolism*; D006207:Half-Life
chemical_list: D005966:Glucuronidase