Interaction of conjugated bile acids and detergents with a radiosorbent assay of vitamin B-12.
The effect of conjugated bile acids and detergents on the radiosorbent technique for the determination of vitamin B-12 activity is reported. It is shown that whereas the non-ionic detergent Triton X-100 has no effect on the vitamin B-12-radiosorbent assay, the addition of ionic detergents (e.g. glycocholic acid, taurocholic acid or sodium laurly sulfate) results in a falsely elevated vitamin B-12 activity presumably due to the disruption of the binding of vitamin B-12 to the intrinsic factor-Sephadex complex. This effect may be of importance not only to the radiosorbent assaying of vitamin B-12, but to the in vivo intestinal absorption of vitamin B-12 as well.
D001647:Bile Acids and Salts / Q000494:pharmacology*; D001665:Binding Sites / Q000187:drug effects; D001667:Binding, Competitive; D002850:Chromatography, Gel; D003037:Cobalt Radioisotopes; D003902:Detergents / Q000494:pharmacology*; D005189:False Positive Reactions; D008722:Methods; D014805:Vitamin B 12 / Q000097:blood*
D001647:Bile Acids and Salts; D003037:Cobalt Radioisotopes; D003902:Detergents; D014805:Vitamin B 12