title: beta-Glucosidase: microbial production and effect on enzymatic hydrolysis of cellulose.
abstract: The enzymatic conversion of cellulose is catalyzed by a multiple enzyme system. The Trichoderma enzyme system has been studied extensively and has insufficient beta-glucosidase (EC 3.2.1.21) activity for the practical saccharification of celluose. The black aspergilli (A. niger and A. phoenicis) were superior producers of beta-glucosidase and a method for production of this enzyme in liquid culture is presented. When Trichoderma cellulase preparations are supplemented with beta-glucosidase from Aspergillus during practical saccharifications, glucose is the predominant product and the rate of saccharification is significantly increased. The stimulatory effect of beta-glucosidase appears to be due to the removal of inhibitory levels of cellobiose.
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology*; D005947:Glucose / Q000096:biosynthesis; D005959:Glucosidases / Q000096:biosynthesis* / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism; D006868:Hydrolysis; D014242:Trichoderma / Q000201:enzymology
chemical_list: D006027:Glycosides; D002482:Cellulose; D005959:Glucosidases; D002480:Cellulase; D005947:Glucose