Cryopreservation of mouse peritoneal macrophages.
A method for the preservation of mouse peritoneal macrophages is described. Using 5% dimethylsulphoxide with either a cooling rate of 1 degree C/min or two-step cooling with 10 min interruption at -30 degrres C allows 80% survival as judged by pinocytic activity. An important finding was that the cells must be handled at 0 degrees C both before and after freezing.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005615:Freezing; D008264:Macrophages; D051379:Mice; D013696:Temperature; D013997:Time Factors
D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide