[Determination and occurence of aflatoxin M1 and B1 in milk and dairy products].
The described method enables a simultaneous identification and determination of aflatoxin M1 and B1 in milk and dairy products by means of a self-registering fluorescence spectrophotometer with a thin-layer chromatographic accessory, directly from the plate. The semiquantitative estimation on the thin-layer chromatographic plate enables also routine analyses in the hygienic practice. The recovery rates for aflatoxin M1 and B1 in milk are about 83 and 82%, respectively -- the detection limit is about 0.1 microng per kg. The recovery rates for aflatoxin M1 and B1 in milk powder are about 89 and 94%, respectively -- the detection limit is bout 0.5 microng per kg. The reproducibility is given with a standard deviation between +/- 1.1 and 6,3% and a variation coeffecient of 1.3 and 6.7, respectively. 4 of 24 analized samples of commercial winter milk were aflatoxin M1-positive, whereas aflatoxin B1 could not be found. One of the milk powder products, a sample of the infant food, "Ki-Na", made in the GDR, contained, however aflatoxin B1. Aflatoxin M1 could not be found. Food-hygienic-toxicological conclusions are discussed.
D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer / Q000379:methods; D003611:Dairy Products / Q000032:analysis*; D005260:Female; D008892:Milk / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D000348:Aflatoxins
Bestimmung und Vorkommen von Aflatoxin M1 und B1 in Milch und Milchprodukten