The effect of prostaglandin F2alpha on the counter-current transfer of 85krypton in the ovarian pedicle of the sheep.
A miniature Geiger-Müller probe was inserted into the corpus luteum in five anesthetized ewes. A similar probe was inserted into either the lumen of the adjacent fallopian tube or the opposite ovary. Overall, 17 infusions of 85Krypton-saline into the uterine vein were carried out in the five subjects. After each two minute infusion into the uterine vein, an increase in radioactivity was registered in the adjacent corpus luteum within 30 - 60 seconds. Maximum radioactivity was registered 1 - 3 minutes after the start of the infusion and within 10 minutes radioactivity had dropped to background [corrected] levels. In two out of three sheep a similar kpattern of 85Krypton transfer was registered in the adjacent fallopian tube. Only a negligible amount of radioactivity above background level was registered in the contralateral control ovary, a result in keeping with the known rapid clearance of 85Krypton from the circulation. There was no indication of any change in pattern of the counter current transfer of 85Krypton during the infusion of PGF2alpha (10 microng/hr) into the ovarian pedicle. These results suggest that the previously observed slow transfer of PGF2alpha itself in the ovarian pedicle may be due to physical rather than pharmacological properties of this prostaglandin.
D000818:Animals; D003338:Corpus Luteum / Q000378:metabolism; D003377:Countercurrent Distribution / Q000295:instrumentation; D005187:Fallopian Tubes / Q000378:metabolism; D005260:Female; D007726:Krypton / Q000378:metabolism*; D010053:Ovary / Q000098:blood supply / Q000378:metabolism*; D011460:Prostaglandins F / Q000494:pharmacology*; D012756:Sheep
D011460:Prostaglandins F; D007726:Krypton