[Sequential demonstration of nucleic acids and protein fractions in the same cells].
Some combinations are suggested to reveal cell components in the following sequence: 1) RNA--DNA--total proteins; 2) RNA--DNA--histones; 3) RNA--DNA--histones--total proteins; 4) RNA--DNA--acidic proteins. RNA is stained with pyronin Y (in the mixture with methyl green). DNA--by the Feulgen procedure (with TCA hydrolysis), histones and total proteins--by Alfert--Geschwind's method and acidic proteins--according to the technique of Smetana and Bush. The details of the whole procedure, adopted for formol-fixed smears are described.
D004247:DNA / Q000378:metabolism*; D006651:Histocytochemistry / Q000379:methods*; D006657:Histones / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*
D006657:Histones; D011506:Proteins; D012313:RNA; D004247:DNA
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