Semiautomated method for the fluorometric determination of total vitamin C in food products.
A simple method employing simultaneous extraction and oxidation has been developed for the semiautomated determination of ascorbic and dehydroascorbic acids in food products. Recovery studies were conducted on ready-to-eat breakfast cereals and both fresh and canned fruits and vegetables, with average recoveries of 101, 100, and 102%, respectively. Reproducibility data were generated showing a relative standard deviation of 3.5%. The automated method was compared with the manual AOAC fluorometric method and with indophenol titration; correlation coefficients were 0.9960 and 0.9926, respectively. The hydrolysis product of dehydroascorbic acid, 2,3-diketogulonic acid, a reported interference in this method, was prepared and shown not to form an interfering fluorescent derivative.
D001205:Ascorbic Acid / Q000032:analysis*; D001322:Autoanalysis / Q000379:methods; D002523:Edible Grain / Q000032:analysis; D005504:Food Analysis; D005638:Fruit / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods; D014675:Vegetables / Q000032:analysis
D001205:Ascorbic Acid