Adsorption chromatography of viruses.
The adsorption isotherm of influenza viruses on wide-pore glass was measured and was found to conform to Langmuir's equation. The affinity constants were estimated at three temperatures and the free energy, enthalpy and entropy of adsorption were calculated. A detailed study showed that the transition from adsorption to total elution is very sharp and proceeds under critical conditions of pH near the pK of the SiOH groups. Therefore, conditions are very favourable for the elution chromatography of viruses. An efficient procedure for the purification and simultaneous concentration of influenza viruses was developed.
D000327:Adsorption; D002845:Chromatography; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D013816:Thermodynamics