Glucagon levels in normal and diabetic subjects: use of a specific immunoabsorbent for glucagon radioimmunoassay.
Non-specific plasma effects may produce major errors in the estimation of true plasma pancreatic glucagon concentrations by radioimmunoassay. This has been circumvented by the production of glucagon-free plasma for each individual investigated, by means of glucagon antibody, coupled to sepharose beads. True fasting pancreatic glucagon levels (mean+/-SEM) in 18 healthy subjects (24+/-3 pg/ml) were significantly lower (p less than .005) than in 10 non-ketotic non-obese diabetics (38+/-3 pg/ml). It is suggested that, in the presence of decreased insulin-effect in the diabetic, this 55% glucagon elevation in diabetics may be of biological importance and contribute to the fasting hyperglycaemia.
D000328:Adult; D000368:Aged; D003920:Diabetes Mellitus / Q000097:blood*; D005260:Female; D005934:Glucagon / Q000097:blood*; D006801:Humans; D007164:Immunosorbents; D008297:Male; D008875:Middle Aged; D011864:Radioimmunosorbent Test / Q000379:methods
D007164:Immunosorbents; D005934:Glucagon