A simple modification to the radiochemical assay of cortisol which avoids the extraction from serum.
A modification to the radiochemical assay method to measure cortisol in serum, that renders unnecessary the extraction of cortisol from a serum sample is described. The serum is heated at 60 degrees C for 3 hours, thereby transcortine is destroyed, then competition binding assay follows as usual.
D005069:Evaluation Studies as Topic; D006358:Hot Temperature; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D011854:Radiochemistry / Q000379:methods*; D014156:Transcortin
D014156:Transcortin; D006854:Hydrocortisone