[Isolation and properties of Micrococcus luteus endonucleas acting in DNA depurinization but inactive with pyrimidine dimers].
An endonuclease (AP-endonuclease II) that specifically attacks double stranded or single stranded depurinated DNA, resulting in single-strand nicks, has been purified 320-fold from Micrococcus luteus. The enzyme is not stimulated by 0.002 M MgCl2, it induces 3'OH-5'PO4 breaks on the 5' side of apurinic sites, it has no activity towards UV-irradiated DNA and has a molecular weight of about 30 000. In cooperation with DNA-polymerase from M. luteus and T4 DNA ligase, AP-endonuclease II has been shown capable of carrying out complete excision repair of depurinated DNA in vitro.
D003090:Coliphages / Q000201:enzymology; D004260:DNA Repair; D004277:DNA, Single-Stranded; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D008836:Micrococcal Nuclease / Q000302:isolation & purification* / Q000378:metabolism; D008837:Micrococcus / Q000201:enzymology; D008970:Molecular Weight; D011118:Polynucleotide Ligases / Q000378:metabolism; D013329:Structure-Activity Relationship
D004277:DNA, Single-Stranded; D004259:DNA-Directed DNA Polymerase; D008836:Micrococcal Nuclease; D011118:Polynucleotide Ligases
Vydelenie i nekotorye svoistva endonukleazy iz Micrococcus luteus, deĭstvuiushcheĭ na depurinizatsii v DNK i neaktivnoĭ po othosheniiu k tsirimidinovym dimeram.