[Optimal conditions for determing ATP of microbial origin].
Endogenous luminescence of the luciferin-luciferase extract from firely tails was studied in determing low concentrations of ATP. The optimum concentration of the the extract, corresponding to the minumum effect of endogenous luminescence, was found in the reaction medium. The solution of luciferin-luciferase was unstable at room temperature and upon dilution. The activity of luciferin-luciferse solutions did not change after freezing in liquid nitrogen with following thawing. The content of ATP was assayed in the cells of Sarcina flava disintegrated with ultrasound.
D000255:Adenosine Triphosphate / Q000032:analysis*; D002021:Buffers; D049454:Firefly Luciferin; D005615:Freezing; D008156:Luciferases; D008722:Methods; D012505:Sarcina / Q000032:analysis
D002021:Buffers; D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D008156:Luciferases
Optimal'nye usloviia opredeleniia ATF mikrobnogo proiskhozhdeniia.