In-situ-isolated perfused rat pancreas: a new method for pharmacological studies of the exocrine pancreas.
A method of in-situ-isolated perfused rat pancreas is described. In situ perfusion of the isolated rat pancreas allows a faster set-up, thanks to a simpler operating procedure. The sensitivity of the isolated rat pancreas has been tested with secretin and cholecystokinin. The pancreas responds to doses of secretin as low as 0.005 CU/kg-h. Such a highly sensitive model might prove to be useful for the bioassay of secretin and the study of the effects of pancreatic secretagogues.
D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002766:Cholecystokinin / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D008722:Methods; D008954:Models, Biological; D010179:Pancreas / Q000378:metabolism* / Q000648:ultrastructure; D010189:Pancreatic Juice / Q000378:metabolism*; D010477:Perfusion; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012633:Secretin / Q000008:administration & dosage / Q000494:pharmacology; D012636:Secretory Rate / Q000187:drug effects
D001639:Bicarbonates; D011506:Proteins; D012633:Secretin; D002766:Cholecystokinin