Bovine peripheral blood monocyte cultures: growth characteristics and cellular receptors for immunoglobulin G and complement.
Bovine peripheral blood monocytes were cultured in vitro for as long as 60 days. Although mitotic figures were not observed, the amount of cytoplasm was observed to increase; most cultures developed multinucleated cells, some containing as many as 200 nuclei. In multinucleated cells, the nuclei were peripherally distributed around a cytoplasmic matrix and surrounded by an undulating membrane. Receptors on macrophages were identified with erythrocytes sensitized with various immunoglobulins and complement (C'). A receptor for immunoglobulin G, but not immunoglobulin M, was detected. By the use of freshly prepared equine serum, a C' receptor was detected; however, by the use of freshly prepared bovine serum, C' receptor was not detected.
D000818:Animals; D001666:Binding Sites, Antibody; D002417:Cattle / Q000097:blood / Q000276:immunology*; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D003168:Complement Fixation Tests; D003165:Complement System Proteins / Q000378:metabolism*; D004912:Erythrocytes / Q000276:immunology; D007074:Immunoglobulin G / Q000378:metabolism*; D066298:In Vitro Techniques; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
D007074:Immunoglobulin G; D003165:Complement System Proteins