Attempts to purify hemopoietic stem cell enrichment in bone marrow by use of glass wool filtration.
This study is about enrichment of hemopoietic stem cell concentration in mouse bone marrow by use of a glass wool filtration technique. Maximum stem cell concentration was accomplished when filtration conditions were as follows: (1) support medium supplemented to 15% with fresh serum from syngeneic donors; (2) no incubation time allowed for cells on the columns; and (3) an operational temperature of 24 degrees C. With these conditions, stem cell concentrations were increased 5,4-fold relative to the concentration in untreated marrow.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002999:Clone Cells; D005374:Filtration / Q000379:methods*; D006412:Hematopoietic Stem Cells; D051379:Mice; D013048:Specimen Handling