Evaluation of three newer methods for investigating protein interactions of penicillin G.
THE INTERACTION OF PENICILLIN G WITH HUMAN SERUM PROTEINS WAS EVALUATED BY THREE DIFFERENT TECHNIQUES: rate of dialysis, cross-linked dextran exclusion, and ultracentrifugation. The rate-of-dialysis technique demonstrated that penicillin G binding to serum was immediate but incompletely reversible. Cross-linked dextran adsorbed or trapped significant amounts of penicillin G, necessitating correction factors of more than 10%. Ultracentrifugation was found to be the most reliable method for quantitative protein-binding determinations of penicillins.
D001798:Blood Proteins / Q000378:metabolism*; D003911:Dextrans; D003956:Dialysis; D066298:In Vitro Techniques; D010400:Penicillin G / Q000378:metabolism*; D011485:Protein Binding; D014461:Ultracentrifugation
D001798:Blood Proteins; D003911:Dextrans; D010400:Penicillin G