title: Studies on the major fucosylated glycoprotein released into the medium by cultured human skin fibroblasts.
abstract: Electrophoretic analysis of the proteins synthesized by cultured human skin fibroblasts demonstrated that the major component released into the medium is a high molecular weight fucosylated glycoprotein (MFGP). Gel filtration chromatography under denaturing and reducing conditions indicated that MFGP has a molecular weight of approx. 250,000; but appears to behave as a disulphide-linked dimer when unreduced. MFGP is further distinguished as the major labelled macromolecule  in the medium after incubation of fibroblasts with [35S]cysteine. The role of this glycoprotein is unknown but it bears a striking resemblance to a presumptive  structural glycoprotein recently shown to be secreted by arterial smooth muscle cells in culture, and may also be related to a group of much studied cell surface glycoproteins.
mesh_terms: D002478:Cells, Cultured; D002850:Chromatography, Gel; D004220:Disulfides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism*; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D008970:Molecular Weight; D012867:Skin / Q000378:metabolism
chemical_list: D004220:Disulfides; D006023:Glycoproteins; D005643:Fucose