[Concentration of sulfhydryl groups and spectral properties of solutions of oxyhemoglobin modified by sodium dodecyl sulfate and UV-irradiation].
The content of sulphydryl groups and spectral properties of mice oxyhemoglobin (a concentration of 3.38 10(-6) M) were studied as affected by sodium dodecyl sulphate in a concentration of 1.38-10(-1) M. It is established that addition of the mentioned detergent to hemoproteid results in an essential decrease in the amount of SH-groups in it and in the disappearance of the globin absorption peak. An integral flow of UV-irradiation (0.36 = 1.8-10(8) erg/cm2) does not change the content of SH-groups in oxyhemoglobin preliminarily modified by sodium dodecyl sulphate; radiation doses of 3.6 = 10.8-10(8) erg/cm2 decrease it. The effect of long-wave UV-irradiation, inducing changes in the heme electron state, on hemoglobin modified by sodium dodecyl sulphate causes a rise in the number of SH-groups determined by amperometric titration. UV-irradiation of modified oxyhemoglobin, in contrast to irradiation of the native oxyhemoglobin, produces no shifts in the Soret band.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004563:Electrochemistry; D051379:Mice; D010108:Oxyhemoglobins / Q000528:radiation effects*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013053:Spectrophotometry; D014466:Ultraviolet Rays
D010108:Oxyhemoglobins; D012967:Sodium Dodecyl Sulfate
Soderzhanie sul'fgidirl'nykh grupp i spektral'nye svoĭstva rastvorov oksigemoglobina, modifitsirovannogo dodetsilsul'fatom natriiia i UF-oblucheniem