Histone mRNA in Xenopus laevis ovaries: identification of the H4 messenger.
RNA extracted from ovaries of adult Xenopus laevis and introduced into a wheat germ cell-free system, directs the synthesis of polypeptides co-electrophoresing with all five histones. By frationation of total ovary RNA through Sephadex G-200 and polyacrylamide gel, RNA was isolated that is translated into histones 4 (H4) only. In addition, this RNA labelled in vitro with [I125] hybridizes to a restriction fragment of cloned sea urchin DNA known to contain the H4 gene.
D000818:Animals; D002474:Cell-Free System; D002999:Clone Cells; D004247:DNA; D005260:Female; D005796:Genes; D006657:Histones / Q000096:biosynthesis; D009693:Nucleic Acid Hybridization; D010053:Ovary / Q000032:analysis*; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D012617:Sea Urchins; D014908:Triticum / Q000378:metabolism; D014981:Xenopus
D006657:Histones; D012333:RNA, Messenger; D012313:RNA; D004247:DNA