Organ culture of human articular cartilage: studies on sulphated glycosaminoglycan synthesis.
An organ culture system is described for adult human articular cartilage obtained from joints after femoral head replacement operations. Cartilage slices maintain maximal viability for 2 days in culture as assessed by uptake of [3H]uridine and [3H]leucine into whole tissue, and 35SO4 into sulphated glycosaminoglycans (GAGs). Since GAGs are the components of cartilage matrix, the depletion of which is associated with osteoarthrosis, a method for measuring sulphated GAG synthesis in culture has been investigated.
D000328:Adult; D002358:Cartilage, Articular; D005270:Femur Head; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D007930:Leucine / Q000378:metabolism; D009924:Organ Culture Techniques / Q000379:methods; D013431:Sulfates / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D006025:Glycosaminoglycans; D013431:Sulfates; D007930:Leucine; D014529:Uridine