title: Adenosine stimulation of fluid transport across rabbit corneal endothelium.
abstract: The rate of fluid transport across rabbit corneal endothelium has been measured with an automatic volumetric method. The present resolution of the procedure is 1-3 nanoliters, and intervals of measurement can be made as small as seconds. In the presence of glucose, oxidized glutathione (GSSG), and adenosine, the maximal rates were 6.2+/-1.0 microliter/hr cm2, and 8.2+/-0.8 microliter/hr cm2 if a large portion of the stroma was dissected away. In the presence of glucose and GSSG only, the rates were lower, namely 3.7+/-0.5 microliter/hr cm2. The rates consistently increased or decreased when adenosine was added or deleted, respectively, during given experiments. The stimulation of fluid transport by adenosine was in the order of 40-50%. The results raise the possibility that this transport mechanism might be subject to metabolic control.
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003315:Cornea / Q000378:metabolism*; D004727:Endothelium / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D008722:Methods; D011817:Rabbits; D012965:Sodium Chloride / Q000378:metabolism*; D012996:Solutions
chemical_list: D012996:Solutions; D012965:Sodium Chloride; D005978:Glutathione; D005947:Glucose; D000241:Adenosine