The phosphoenolpyruvate-dependent phosphotransferase system of Staphylococcus aureus. 1. Amino-acid sequence of the phosphocarrier protein HPr.
The primary structure of the histidine-containing phosphocarrier protein HPr of the phosphoenolpyruvate-dependent phosphotransferase system from Staphylococcus aureus was determined by automated Edman degradation. The complete sequence was deduced from the direct analysis of the protein by automated Edman degradation in a liquid-phase sequencer of Edman and from the sequence of tryptic, thermolytic and cyanogen bromide peptides as obtained by automated Edman degradation in a solid-phase sequencer of Laursen. The amino-acid sequence was found to be Met-Glu-Gln-Asn-Ser-Tyr-Val-Ile-Ile-Asp-Glu-Thr-Gly-Ile-His-Ala-Arg-Pro-Ala-Thr-Met-Leu-Val-Gln-Thr-Ala-Ser-Lys-Phe-Asp-Ser-Ile-Asp-Gln-Gly-Gly-Tyr-Asp-Ser-Met-Gln-Leu-Lys-Ser-Leu-Gly-Val-Gly-Lys-Asp-Glu-Glu-Ile-Thr-Ile-Tm-Ser-Ala-Asp-Lys-Lys-Glu-Gly-Leu-Thr-Lys-Met-Ser-Ile-Val. The 70 residues correspond to a molecular weight of 7685. The one histidine involved in the phosphotransfer reaction of this protein was found at position 15 as part of a region of the sequence which has no predictable secondary structure. It is suggested that this protein belongs to the group of male proteins with the active center located on a protrusion rather than a cleft.
D000595:Amino Acid Sequence; D000626:Aminopeptidases; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D002942:Circular Dichroism; D003488:Cyanogen Bromide; D010446:Peptide Fragments / Q000032:analysis; D010710:Phosphates / Q000378:metabolism*; D010728:Phosphoenolpyruvate / Q000494:pharmacology; D010770:Phosphotransferases / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D013211:Staphylococcus aureus / Q000378:metabolism*; D013820:Thermolysin; D014357:Trypsin
D001426:Bacterial Proteins; D002352:Carrier Proteins; D010446:Peptide Fragments; D010710:Phosphates; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases; D000626:Aminopeptidases; D014357:Trypsin; D013820:Thermolysin; D003488:Cyanogen Bromide