Synthesis of a compound soluble in organic solvents from deoxycytidine triphosphate in permeabilized normal human lymphocytes.
Normal human lymphocytes may be rendered permeable to deoxynucleoside triphosphates. When [3H]dCTP is furnished to permeabilized lymphocytes tow compounds are formed: DNA and a compound soluble in organic solvents. [3H]dCTP incorporation is higher in stimulated lymphocytes than in unstimulated cells. Some characteristics of the system are reported.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D003597:Cytosine Nucleotides / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D003843:Deoxycytidine Monophosphate / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D010539:Permeability; D012997:Solvents
D003597:Cytosine Nucleotides; D037102:Lectins; D012997:Solvents; D003843:Deoxycytidine Monophosphate; D000255:Adenosine Triphosphate; D004247:DNA; D008274:Magnesium; D002118:Calcium