title: Molecular sizes and relationships of TOL plasmids in Pseudomonas.
abstract: Plasmid deoxyribonucleic acid was isolated from thirteen Pseudomonas strains judged on genetic criteria to carry plasmids coding for the degradation of toluene and m- and p-xylenes (TOL plasmids). Most strains carried a single species, but two strains carried two size classes, and cells of a third strain contained plasmids ranging in size from 25 X 10(6) to 202 X 10(6) daltons. Some plasmids could be transformed into a Pseudomonas putida strain to yield Tol+ progeny. Plasmids from 5 of the 13 strains were indistinguishable on the basis of size and gel pattern of fragments after endonuclease digestion.
mesh_terms: D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005111:Extrachromosomal Inheritance; D010641:Phenotype; D010957:Plasmids; D011549:Pseudomonas / Q000032:analysis / Q000378:metabolism / Q000648:ultrastructure*; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes