Platelet activation in haemostasis: role of thrombin and other clotting factors in platelet-collagen interaction.
The mechanism of activation of platelets by collagen was examined. Hirudin interfered with the initial collagen-platelet interaction and both hirudin and heparin inhibited collagen-induced release of platelet granular contents. Hirudin completely inhibited the release of both [3H]5HT and beta-glucuronidase whereas heparin completely inhibited release of beta-glucuronidase but only partly inhibited release of [3H] 5HT. beta-Glucuronidase and maximal [3H] 5HT were only released when plasma was present. The results are compatible with an essential intermediary role for thrombin in collagen activation of platelets. Evidence was also obtained that von Willebrand factor may participate in this reaction.
D001779:Blood Coagulation Factors; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D003094:Collagen / Q000502:physiology*; D005966:Glucuronidase / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D006629:Hirudins / Q000494:pharmacology; D006801:Humans; D008297:Male; D010974:Platelet Aggregation; D012701:Serotonin / Q000378:metabolism; D013917:Thrombin / Q000502:physiology*
D001779:Blood Coagulation Factors; D006629:Hirudins; D012701:Serotonin; D006493:Heparin; D003094:Collagen; D005966:Glucuronidase; D013917:Thrombin