Proflavine sensitivity of RNA processing in isolated nuclei.
The intercalating agent proflavine inhibits the processing and subsequent release of preformed messenger RNA and ribosomal RNA from isolated liver nuclei to surrogate cytoplasm. The direct effect of proflavine on these processes, as monitored in a reconstituted cell-free system, supports the theory that base-paired segments (i.e. hairpin loops) in the precursor RNA's are involved as recognition sites in nuclear RNA processing.
D000166:Acridines / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites; D001692:Biological Transport / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D003593:Cytoplasm / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008115:Liver Regeneration; D011370:Proflavine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D051381:Rats
D000166:Acridines; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011370:Proflavine