title: Analysis of ochratoxin B alone and in the presence of ochratoxin A, using carboxypeptidase A.
abstract: A method is described for ochratoxin B analysis, which is adapted to the earlier described method of ochratoxin A analysis, using carboxypeptidase A (K. Hult and S. Gatenbeck, J. Assoc. Off. Anal. Chem. 59:128-129, 1976). The fluorescence spectra of ochratoxins A and B coincide too much to allow direct discrimination of the two compounds. A method using the differences in kinetic parameters of the enzymatic hydrolysis of the two compounds is suggested for the analysis of mixtures of the ochratoxins.
mesh_terms: D002268:Carboxypeptidases; D008433:Mathematics; D008722:Methods; D009793:Ochratoxins / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D009793:Ochratoxins; D002268:Carboxypeptidases