The low temperature magnetic circular dichroism spectra of iron-sulphur proteins. II. Two-iron ferredoxins.
Variable temperature magnetic circular dichroism (MCD) spectra of a number of two-iron ferredoxins have been measured. The spectra of fully oxidised spinach and Spirulina maxima ferredoxin are independent of temperature between room temperature and 18 K, showing that no contribution to the room temperature MCD spectrum arises from the small population of low-lying excited states originating from the exchange coupling. However, the low temperature MCD spectra of the half-reduced proteins spinach and Spirulina maxima ferredoxin and adrenodoxin are all reasonably intense and temperature dependent. An interpretation of the spectrum of the charge-transfer region is suggested by starting with the assignments previously obtained from rubredoxin.
D002942:Circular Dichroism; D005288:Ferredoxins; D005615:Freezing; D008280:Magnetics; D010084:Oxidation-Reduction; D010944:Plants; D011487:Protein Conformation
D005288:Ferredoxins