Quantitation of dimethylnitrosamine in the whole mouse after biosynthesis in vivo from trace levels of precursors.
A simple and highly sensitive procedure is described for the recovery and quantitative identification of nanogram quantities of preformed N-nitroso compounds in the whole mouse. This procedure has also been applied to the quantitation of N-nitroso compounds after they have been biosynthesized from trace amounts of precursors. The whole animal is frozen in liquid nitrogen and homogenized to a frozen powder; the powder is then extracted and analyzed by a thermal energy analyzer interfaced to a gas-liquid and a high-pressure liquid chromatograph.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004123:Dimethylamines / Q000378:metabolism*; D004128:Dimethylnitrosamine / Q000032:analysis / Q000378:metabolism*; D005615:Freezing; D008297:Male; D051379:Mice; D009573:Nitrites / Q000378:metabolism*; D009602:Nitrosamines / Q000378:metabolism*; D013997:Time Factors
D004123:Dimethylamines; D009573:Nitrites; D009602:Nitrosamines; D004128:Dimethylnitrosamine