Stability of the antigen expression in the spontaneous mammary adenocarcinoma during cultivation and absorbtion with immune serum.
Transplantable spontaneous mammary adenocarcinoma cells were cultivated in vitro under standard cell culture conditions, in presence of extracellular protease, in cottonstoppered bottles, and in presence of antitrypsin. At time intervals, aliquots from each were removed and examined for transplantability, response to humoral antibody, and to cytotoxic activity of spleen cells from immune animals. Under standard conditions, cells cultivated for 300 days or more became non-transplantable and 74% survived the killing effect of immune spleen cells. In presence of an extracellular protease, the cells maintained their transplantability and 23% survived the cytotoxic effects of the immune spleen cells. In cotton-stoppered glass bottles without HEPES, the cells maintain their transplantability and they survived the killing effect of the immune spleen cells. Finally, in presence of antitrypsin, the cells became less transplantable, remained sensitive to the toxic effects of the immune spleen cells. When absorbed with adenocarcinoma anti-serum, cells cultivated under standard cell culture conditions, or cultured in presence of antitrypsin became less transplantable, whereas, cells grown in cotton-stoppered glass bottles or in presence of the extracellular protease maintained their transplantability.
D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D006023:Glycoproteins / Q000302:isolation & purification; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology; D008565:Membrane Proteins / Q000302:isolation & purification; D051379:Mice; D009368:Neoplasm Transplantation; D010447:Peptide Hydrolases / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D014361:Trypsin Inhibitors / Q000494:pharmacology
D000906:Antibodies; D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D006023:Glycoproteins; D008565:Membrane Proteins; D014361:Trypsin Inhibitors; D010447:Peptide Hydrolases