Simplified radioimmunoassay for viral antigens: use of Staphylococcus aureus as an adsorbent for antigen-antibody complexes.
In a rapid method for the radioimmunoassay (RIA) of viral antigens, Staphylococcus aureus was used as the adsorbent for antigen-antibody complexes ("protein A" molecules on the cell walls of certain strains of staphylococci have a strong affinity toward IgG molecules). The results showed that this method could be used instead of the double-antibody technique, with the same or probably higher sensitivity in precipitation as well as competition RIA's.
D000327:Adsorption; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral / Q000032:analysis*; D001667:Binding, Competitive; D007074:Immunoglobulin G / Q000378:metabolism; D066298:In Vitro Techniques; D011863:Radioimmunoassay / Q000379:methods*; D011925:Rauscher Virus / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D007074:Immunoglobulin G