Human interferon: mass production in a newly established cell line, MG-63.
MG-63 cells, a line derived from an osteosarcoma, produced high yields of interferon after superinduction with polyinosinic acid.polycytidylic acid, cycloheximide, and actinomycin D. Advantages of MG-63 cells over diploid fibroblasts as a substrate are: no requirement for aging between confluency and induction, no requirement for priming, and 3.7-fold higher yields per square centimeter of culture surface. Physicochemically and biologically, MG-63 cell interferon resembles fibroblast rather than leukocyte interferon.
D002460:Cell Line; D002478:Cells, Cultured; D004171:Diploidy; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007372:Interferons / Q000096:biosynthesis*; D009369:Neoplasms / Q000378:metabolism; D009500:Neutralization Tests; D012867:Skin / Q000166:cytology
D007372:Interferons