Sesquiterpene lactones. XIX. Preliminary studies on the mode of action. Inhibition of synthesis of tumor cell protein and RNA.
The isotopic examinations of the mode of action of cytostatic active acid 8a-isobuetyric-14,15-dihydroxy-germacran-1 (10),4,11(13)-trienolide 12(6) (alatolide) have demonstrated that the compound at concentration 5-6 X 10(-5) M induces defective changes in HeLa cells resulting in the inhibition of protein synthesis in 93-8% and RNA in 91-9% and, in consequence, cell death. At lower concentrations 2-8 X 10(-6) M, the compound inhibits protein synthesis in 73% and RNA in 5-7%. The direct influence of alatolide on DNA synthesis is doubtful.
D002460:Cell Line; D003864:Depression, Chemical; D005260:Female; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D007783:Lactones / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D012717:Sesquiterpenes / Q000494:pharmacology*; D014158:Transcription, Genetic
D007783:Lactones; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D012717:Sesquiterpenes