title: Nuclear and cytoplasmic genome relationships in the genus Avena: analysis by isoelectric focusing of ribulose biphosphate carboxylase subunits.
abstract: Comparisons of the isoelectric points of small and large subunits of ribulose biphosphate carboxylase extracted from a number of diploid, tetraploid, and hexaploid Avena species have been used to obtain information on the nuclear and cytoplasmic genome relationships within the genus. All species tested had small subunits with similar isoelectric points, so their analysis provided no information of taxonomic value. Three types of large subunits could be distinguished by this method, and the distribution of each among the available species provides strong evidence against the involvement of a C genome diploid (such as A. ventricosa) as the maternal parent in the formation of either tetraploid or hexaploid species. One type of large subunit was confined to the perennial tetraploid, A. macrostachya, and its position in the genus and possible origin are discussed. The value of this approach in studying genome relationships within the genus Avena and related genera is assessed.
mesh_terms: D002262:Carboxy-Lyases / Q000302:isolation & purification; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D002523:Edible Grain / Q000201:enzymology; D005796:Genes; D007461:Iodoacetates / Q000494:pharmacology; D007525:Isoelectric Focusing; D046911:Macromolecular Substances; D010944:Plants / Q000201:enzymology*; D011485:Protein Binding; D012273:Ribulose-Bisphosphate Carboxylase / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D007461:Iodoacetates; D046911:Macromolecular Substances; D002262:Carboxy-Lyases; D012273:Ribulose-Bisphosphate Carboxylase