Determination of morphine by electron capture gas-liquid chromatography.
We describe a micromethod for determining as little as 0.1 microgram of morphine per liter of human urine. The procedure is about 20-fold more sensitive than are currently used gas-liquid chromatographic methods. It is particularly suitable for use as a confirmatory method for large-scale radioimmunoassay screening tests. The procedure involves extraction of morphine from urine and analysis of its heptafluorobutyryl derivative by gas-liquid chromatography with electron capture detection. Morphine can be determined by this procedure over a range of 0.1-200 microgram/liter of urine, 2 pg of the pure derivative being the lowest amount detectable. Urine samples from patients receiving methadone were analyzed by thin-layer chromatography, radioimmunoassay, and our procedure. The results by our procedure agreed well with those obtained by radioimmunoassay.
D002849:Chromatography, Gas / Q000379:methods; D003061:Codeine / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008832:Microchemistry; D009020:Morphine / Q000652:urine*; D011863:Radioimmunoassay / Q000379:methods
D009020:Morphine; D003061:Codeine