Studies on the formation of alpha-amylase by Thermomonospora vulgaris.
Conditions affecting the formation of alpha-amylase by static cultures of the thermophilic actinomycete Thermomonospora vulgaris were studied. The organism failed to grow under submerged culture conditions or when the culture medium was devoid of CaCO3-alpha-Amylase was produced during the logarithmic phase of growth and maximum yield was obtained after 3 to 9 days of incubation. Growth and amylase formation took place only in a range from 45 degrees to 55 degrees C; optimum temperature was 55 degrees C. Of the tested carbon sources only starch induced enzyme formation. Maximum enzyme yield was obtained when starch concentration of the medium was 2% and when ammonium citrate served as a nitrogen source. Crushed clay pots could substitute for CaCO3 of the medium, but growth and amylase yield were less.
D000681:Amylases / Q000096:biosynthesis* / Q000378:metabolism; D002119:Calcium Carbonate / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002474:Cell-Free System; D002951:Citrates; D003470:Culture Media; D008848:Micromonosporaceae / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D013696:Temperature
D002951:Citrates; D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D013213:Starch; D000681:Amylases; D002119:Calcium Carbonate; D009584:Nitrogen