Simultaneous identification and quantitation of diphenhydramine and methaqualone.
Methods for the simulataneous identification and quantitation of diphenhydramine and methaqualone in urine have been investigated. Suitable thin-layer and gas chromatographic techniques have been developed. The extraction techniques were evaluated by extracting human urine after the ingestion of therapeutic dosages of the two drugs. A charcoal cartridge technique proved to be superior to direct solvent extraction. Although enzyme hydrolysis gave slightly better recoveries than the charcoal cartridge technique, it is a time-consuming procedure.
D000818:Animals; D002606:Charcoal; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004155:Diphenhydramine / Q000008:administration & dosage / Q000652:urine*; D004338:Drug Combinations; D006801:Humans; D007274:Injections, Intraperitoneal; D008297:Male; D008702:Methaqualone / Q000008:administration & dosage / Q000652:urine*; D008722:Methods; D051381:Rats
D004338:Drug Combinations; D002606:Charcoal; D008702:Methaqualone; D004155:Diphenhydramine