title: Evidence for the presence of phosphoriboisomerase and ribulose-1,5-diphosphate carboxylase in extracts of Desulfovibrio vulgaris.
abstract: Cell extracts of Desulfovibrio vulgaris were found to incorporate 14CO2 into acid-stable products when ribose-5-phosphate or ribulose-1,5-diphosphate was used as a substrate. This CO2 fixation required adenosine triphosphate and produced 3-phosphoglyceric acid as one of the products. The assimilation of CO2 by pentose phosphates was unrelated to the pyruvate-CO2 exchange reaction. The pyruvate-CO2 exchange did not require adenosine triphosphate, did not produce phosphorylated compounds, and, unlike the pentose phosphate system, required an acidic protein fraction for activity.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D002474:Cell-Free System; D003901:Desulfovibrio / Q000201:enzymology*; D005988:Glyceric Acids / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D012267:Ribosemonophosphates / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*; D012274:Ribulosephosphates / Q000378:metabolism
chemical_list: D005988:Glyceric Acids; D011773:Pyruvates; D012267:Ribosemonophosphates; D012274:Ribulosephosphates; D002245:Carbon Dioxide; D000255:Adenosine Triphosphate; D002262:Carboxy-Lyases; D012273:Ribulose-Bisphosphate Carboxylase; D002238:Carbohydrate Epimerases