Effect of thymidine and thymidylate analogs on nucleic acid synthesis in tumor cells.
Synthetic 5'-amino-5'-deoxythymidine (5'-AdThd), alpha,beta-methylenethymidine diphosphate (alpha,beta-MTDP), and alpha,beta-methylenethimidine triphosphate (alpha,beta-MTTP) were found to inhibit thymidine kinase. Using thymidine kinase extracted from FM 3A/B cells (a strain of mouse mammary gland tumor cells), the Ki values of 5'-AdThd, alpha,beta-MTDP, and alpha,beta-MTTP against thymidine were calculated to be 9.2 X 10(-5)M, 2.3 X 10(-5) M, and 1.8 X 10(-5) M, respectively. At concentrations above their Ki values alpha,beta-MTDP and alpha,beta-MTTP did not inhibit incorporation of labeled thymidine into DNA of cultured cells, whereas 5'-AdThd did. Under the same conditions all three compounds inhibited TMP incorporation. The inhibitions of thymidine and TMP incorporation were specific, since the incorporation of deoxyguanosine was scarcely inhibired by 5'-AdThd. These results suggested that the specific inhibition of thymidine and TMP incorporation was mostly due to reduction in permeability of the cells to these substrates rather than to inhibition of thymidine kinase activity.
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D051379:Mice; D012313:RNA / Q000096:biosynthesis; D012517:Sarcoma, Yoshida / Q000032:analysis; D013936:Thymidine / Q000494:pharmacology*; D013937:Thymidine Kinase / Q000037:antagonists & inhibitors; D013938:Thymidine Monophosphate / Q000494:pharmacology*; D013942:Thymine Nucleotides / Q000494:pharmacology*
D013942:Thymine Nucleotides; D013938:Thymidine Monophosphate; D012313:RNA; D004247:DNA; D013937:Thymidine Kinase; D013936:Thymidine