Identification of retinal isomers isolated from bacteriorhodopsin.
The purple membrane of Halobacterium halobium contains the protein bacteriorhodopsin which resembles the visual pigment, rhodopsin, in many aspects. The isomeric configurations of its chromophore, retinal, were studied by a combination of methylene chloride extraction and analysis by high-pressure liquid chromatography. The light-adapted form bR570LA yields solely all-trans-retinal, while the dark-adapted form of bacteriorhodopsin, bR560DA, yields a mixture of 13-cis and all-trans with a ratio of similar to 1;1. The photointermediate M412 in a membrane modified by ether at high NaCl concentration also yields an approximately 1:1 mixture of 13-cis-and all-trans-retinals, while a similar M405 species produced by illumination in 2 M guanidine hydrochloride at high pH yields mainly 13-cis-retinal. These results indicate that the photochemical cycle of bR570LA may involve an isomerization of the retinal chromophore from the all-trans to the 13-cis form.
D001436:Bacteriorhodopsins; D002338:Carotenoids; D003623:Dark Adaptation; D006217:Halobacterium; D008027:Light; D012172:Retinaldehyde / Q000032:analysis*; D013237:Stereoisomerism; D014801:Vitamin A / Q000031:analogs & derivatives*
D014801:Vitamin A; D002338:Carotenoids; D001436:Bacteriorhodopsins; D012172:Retinaldehyde