[Quantitative method of determining the activity of multiple forms of superoxide dismutase].
A quantitative method for determination of the activity of multiple superoxide dismutase (SOD) forms is presented. The method is based on SOD electrophoretic separation on polyacrylamide gels, elution from gels and determination of inhibition of nitro blue tetrazolium reduction in the NADH2 + phenazinemetasulphate system. As shown, partially purified SOD from bovine erythrocytes separated into three fractions. The activity of form 2 proved to be much lower than the degree of its staining for protein.
D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology; D013053:Spectrophotometry / Q000379:methods; D013482:Superoxide Dismutase / Q000032:analysis*
D013482:Superoxide Dismutase
Kolichestvennyĭ metod opredeleniia aktivnosti mnozhestvennykh form superoksiddismutazy.