Fluorescent thiol reagents. XII. Fluorescent tracer method for protein SH groups using N-(7-dimethylamino-4-methyl coumarinyl) maleimide. An application to the proteins separated by SDS-polyacrylamide gel electrophoresis.
D000199:Actins / Q000032:analysis; D000418:Albumins / Q000032:analysis; D000681:Amylases / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008301:Maleimides; D008623:Mercaptoethanol; D009218:Myosins / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D011489:Protein Denaturation; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents
D000199:Actins; D000418:Albumins; D008301:Maleimides; D010446:Peptide Fragments; D011506:Proteins; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol; D000681:Amylases; D009218:Myosins