Defibrination of normal human blood in vitro: a method giving a high recovery of untraumatized cells.
A new automatic apparatus is described for the defibrination of blood in vitro at laboratory temperature which results in only small (less than 10%) losses of white cells. Freshly-drawn blood is mixed smoothly at high speed (greater than 1200 rpm) and fibrin is removed rapidly as formed. The apparatus is designed to produce minimum mechanical trauma to the blood. Subsequent in vitro incubation of red cells, granulocytes and lymphocytes demonostrated their viability to be comparable with cells obtained by other methods of defibrination.
D001773:Blood Cells; D002469:Cell Separation; D002470:Cell Survival; D004912:Erythrocytes; D005337:Fibrin; D006405:Hematology / Q000295:instrumentation / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes
D005337:Fibrin