Quantitation of the antimalarial agent, mefloquine, in blood, plasma, and urine using high-pressure liquid chromatography.
Sensitive and specific assays are described for the quantitation of mefloquine in whole blood, plasma, and urine specimens using high-pressure liquid chromatography. Specimens were extracted with ethyl acetate and concentrated before chromatography. Whole blood and plasma extracts were chromatographed on a polar bonded phase partitioning column, and urine extracts were chromatographed on a bonded reversed-phase partitioning column. The sensitivity of the assays for mefloquine was 0.05 microgram/ml of whole blood or plasma and 0.25 microgram/ml of urine using 5-ml samples. The assays are suitable for studying mefloquine pharmacokinetics in humans.
D000328:Adult; D000962:Antimalarials / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D008722:Methods; D010880:Piperidines / Q000032:analysis / Q000097:blood / Q000652:urine; D010949:Plasma / Q000032:analysis; D011804:Quinolines / Q000032:analysis* / Q000097:blood / Q000652:urine; D013997:Time Factors
D000962:Antimalarials; D010880:Piperidines; D011804:Quinolines