[Possiblity of using immobilized urease for degradation of urea in blood plasma].
As revealed the velocities of urea decomposition in the citrate donor plasma with soluble urease and urease immobilized by addition to carboxymethyl ester of cellulose, 2-(3'-amino-4'-methoxyphenyl)-sulphonylethyl ester of cellulose, diethylaminoester of cellulose, stained with dichlortriazine stain, or graft copolymere of cellulose and polyglycidylmetacrylate were sufficiently close to one another. Preparations of immobilized urease can be repeatedly used for urea decomposition in the citrate donor blood. Periodical treatment of the mentioned preparations with cystein solution led to a lesser decrease of enzymatic activity of the immobilized urease after repeated use.
D001793:Blood Preservation; D004800:Enzymes, Immobilized; D006801:Humans; D014508:Urea / Q000097:blood*; D014510:Urease
D004800:Enzymes, Immobilized; D014508:Urea; D014510:Urease
Issledovanie vozmozhnosti ispol'zovaniia immobilizobannoĭ ureazy dlia razlozheniia mocheviny v plazme krovi