A specific direct radioimmunoassay for oestriol-16alpha-glucuronide in pregnancy plasma.
A specific radioimmunoassay has been developed for measuring oestriol-16alpha glucuronide in pregnancy plasma using a highly specific antiserum. The specificity of this antiserum has been assessed by the 50% displacement method and by measuring oestriol-16alpha-glucuronide concentrations in pregnancy plasma samples, both with and without chromatography. The antiserum was found to have Ka of 1.3 X 10(10) M-1. The assay had a sensitivity of 5 pg (least quantity of oestriol-16alpha-glucuronide distinguishable from the zero point, p less than 0.05), an intra-assay variation of 6.07% and an inter-assay variation of 3.36%. The assay was used to measure plasma oestriol-16alpha-glucuronide concentrations throughout eight pregnancies, five of which were normal and three had some defined abnormality.
D003429:Cross Reactions; D004964:Estriol / Q000097:blood*; D005260:Female; D005965:Glucuronates / Q000097:blood; D006801:Humans; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods
D005965:Glucuronates; D004964:Estriol