Isolation and characterization of the sulfated glycosaminoglycans of the vitreous body.
Ester sulfate containing glycosaminoglycans comprising approx. 3% of the total glycosaminoglycan content, have been isolated from protease-digested bovine vitreous body by stepwise fractionation on AG-1X2(Cl-) and gel filtration on Bio-Gel P-300. Two heparan sulfate and two chondroitin-4-sulfate fractions were isolated in nearly pure form. The heparan sulfate fractions were undersulfated and contained the same relative proportions of N- and O-sulfate (1:2), although the total sulfate content differed by approx. 100%. No chondroitin-6-sulfate was present in the isolates, based on evidence obtained from chondroitin ABC lyase experiments.
D000818:Animals; D002417:Cattle; D005688:Galactosamine / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006025:Glycosaminoglycans / Q000302:isolation & purification; D012694:Serine / Q000032:analysis; D013464:Sulfuric Acids / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis; D014822:Vitreous Body / Q000032:analysis*; D014994:Xylose / Q000032:analysis
D006025:Glycosaminoglycans; D013464:Sulfuric Acids; D014574:Uronic Acids; D012694:Serine; D005688:Galactosamine; D014994:Xylose; D005944:Glucosamine; D005690:Galactose