Isolation of lung alveolar walls.
Disintegration of peripheral lung tissue followed by filtration through an 80 mesh stainless steel screen yields a filtrate containing tissue particles with properties consistent with their identification as fragments of alveolar walls. Application of this procedure readily yields large quantities of alveolar wall tissue.
D000367:Age Factors; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002469:Cell Separation / Q000379:methods; D005374:Filtration; D006901:Hydroxylysine / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D008854:Microscopy, Electron; D011650:Pulmonary Alveoli / Q000032:analysis / Q000648:ultrastructure
D000596:Amino Acids; D006901:Hydroxylysine; D006909:Hydroxyproline