Studies on acid eluates from kidneys of sheep with glomerulonephritis mediated by antibody to glomerular basement membrane.
Kidneys from six sheep having glomerulonephritis mediated by antibody to glomerular basement membrane (GBM) were extracted at acid pH. Each preparation was characterised using immunological techniques and the eluates contained between 3.6 and 13% anti-GBM antibody of IgG class. It appears that this low antibody content is due to the presence of contaminants, mainly serum proteins.
D000143:Acids; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification; D000918:Antibody Specificity; D000941:Antigens / Q000008:administration & dosage; D001485:Basement Membrane / Q000276:immunology*; D005921:Glomerulonephritis / Q000209:etiology / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis* / Q000302:isolation & purification; D007668:Kidney / Q000276:immunology; D012756:Sheep
D000143:Acids; D000906:Antibodies; D000941:Antigens; D007074:Immunoglobulin G