Stimulation of hepatic lipogenesis by eicosa-5,8,11,14-tetraynoic acid in mice fed a high linoleate diet.
Liver slices, from mice fasted for one day and then refed for three days either a 15% corn oil diet or a 15% corn oil diet containing eicosa-5,8,11,14-tetraynoic acid (TYA), were incubated with [1-14C] acetate or [3H] H2O to determine lipogenic capacity. Dietary TYA produced a twofold stimulation in fatty acid and cholesterol synthesis. TYA also caused an increase in the relative proportion of linoleate (C18:2) and a decrease in that of arachidonate (C20:4) in liver. Thus, (a) despite high levels of C18:2, hepatic lipogenesis can be increased, and (b) even short term feeding of TYA can alter the hepatic fatty acid composition presumably by inhibition of arachidonate synthesis from linoleate.
D000818:Animals; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004537:Eicosanoic Acids / Q000494:pharmacology*; D005215:Fasting; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D005260:Female; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D013268:Stimulation, Chemical
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004537:Eicosanoic Acids; D005231:Fatty Acids, Unsaturated