title: Lipids of the yeast Hansenula anomala.
abstract: An analysis of the free lipids of Hansenula anomala was performed. The main fatty acids obtained by saponification of whole cell crude lipids were palmitic, C18:1, C18:2 and C18:3 acids. In mitochondrial lipids the tri-unsaturated acid was present as traces. Fatty acid composition of each class of lipids was also determined. Phosphatidylcholine and phosphatidylethanolamine were the main phospholipids; phosphatidylserine, phosphatidylinositol and cardiolipin were also characterized. The most abundant sterols were ergosterol and lanosterol. An acetate of a 24-ethyl cholesterol was also isolated. Two glycolipids, a galactosyl diglyceride and a glucosyl ceramide were identified; concerning the galactosyl diglyceride, the content of C18:3 acid was higher than in other lipid classes. In the glucosyl ceramide, the main fatty acid was alpha-hydroxy C18:0 acid; C16:0, C18:1, C18:2 and C18:3 acids were present too. The long chain base was shown to be C18-phytosphingosine (4-hydroxy C18 sphinganine). Some similarities and differences with Saccharomyces cerevisiae are discussed.
mesh_terms: D001203:Ascomycota / Q000032:analysis*; D002518:Ceramides / Q000032:analysis; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification; D013058:Mass Spectrometry; D010743:Phospholipids / Q000032:analysis; D010843:Pichia / Q000032:analysis*; D013045:Species Specificity; D013110:Sphingosine / Q000032:analysis
chemical_list: D002518:Ceramides; D005230:Fatty Acids, Nonesterified; D006017:Glycolipids; D008055:Lipids; D010743:Phospholipids; D013110:Sphingosine