title: Deoxyribonucleic acid relationships among marine vibrios pathogenic to fish.
abstract: Polynucleotide sequence relationships among 19 strains of marine vibrios, 15 of which were pathogenic to fish, were assessed by analysis of DNA-DNA homo-and hetero-duplexes with the endonuclease S1. DNA base compositions (mole% guanine-cytosine (GC) of selected vibro strains were determined by thermal denaturation. Pathogenic strains from the Pacific Northwest had identical mole% GC but could be divided into two discrete, yet related, DNA-homology groups. One highly related group was typical of Vibro anguillarium and showed greater than 70% within-group polynucleotide sequence homology. Second group (designated V1669) showed greater than 83% within-group homology but only about 60% relatedness to V. anguillarum. Neither group showed a significant degree of relatedness to V. parahaemolyticus (biotypes parahaemolyticus or alginolyticus) or to representative strains of any of the other previously described homology groups which we tested.
mesh_terms: D000818:Animals; D001483:Base Sequence; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis*; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes; D006147:Guanine / Q000032:analysis; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012623:Seawater; D014733:Vibrio / Q000145:classification* / Q000472:pathogenicity; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D006147:Guanine; D003596:Cytosine