[Study of microcirculation of the human mouth mucosa by the method of contact microscopy].
The work presents a new installation and a method for intravital investigation of microcirculation in man. The installation is a modification of the contact fluorescent microscope MLK-1 produced by the Leningrad Optico-Mechanical Amalgamation which consists of the introduction of a polarization light-dividing prism into the optical scheme of the microscope for examination of the object in polarized falling rays and of substitution of the illumination source of the microscope (lamp KLM9-75) for the xenon lamp DKSSh-120, working both in the standard and given impulse regimen. The latter opens opportunties for determination of the blood flow rate in capillaries. The determination principle is that the effect of bloodflow "stop" is observed in the case of "coincidence" of the bloodflow in the capillary and the lamp modulation rate. The mucouse membrane of the oral cavity (cheek) was used as the object for investigation. It has been shown that its microcirculatory bed in normality is represented by capillary loops in the shape of a hair-pin having the diameter of 2;92--7,74 mkm. Their amount in the view field fluctuates between 8 and 20. The average blood flow rate in the capillaries under study is 0,4 mm/sec.
D001783:Blood Flow Velocity; D002196:Capillaries / Q000502:physiology*; D006801:Humans; D008833:Microcirculation; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D008954:Models, Biological; D009061:Mouth Mucosa / Q000098:blood supply*
Issledovanie mikrotsirkuliatsii v slizistoĭ obolochke shcheki cheloveka metodom kontaktnoĭ mikroskopii