Coenzyme-A-dependent esterification of cholesterol in intestinal mucosa from guinea-pig. Influence of diet on the enzyme activity.
Guinea-pig intestinal mucosa contains a microsomal enzyme which catalyses the esterification of cholesterol. The enzyme is CoA-dependent and probably an acyl-CoA-cholesterol acyltransferase (ACAT) (E.C. 2.3.1.26). The apparent Km for the CoA in the combined enzyme reaction is 3.3 x 10(-5)M. The specific activity of cholesterol esterification is about five times higher in young suckling animals than in ordinary fed adult animals. Feeding fat and fat/cholesterol diets increases the activity two- to five-fold. Concomitantly the fat/cholesterol fed animals get a marked accumulation of free and esterified cholesterol in mucosal cells.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002784:Cholesterol / Q000378:metabolism* / Q000494:pharmacology; D002788:Cholesterol Esters / Q000378:metabolism; D003065:Coenzyme A; D004041:Dietary Fats; D004951:Esterification; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000201:enzymology / Q000378:metabolism; D002785:Sterol O-Acyltransferase / Q000378:metabolism*
D002788:Cholesterol Esters; D004041:Dietary Fats; D002784:Cholesterol; D000217:Acyltransferases; D002785:Sterol O-Acyltransferase; D003065:Coenzyme A