Effect of diet on the metabolism of labelled tocopherol in sheep.
1. Eighteen crossbred wethers were allotted at random (six per treatment) to each of the following diets: (1) maize-silage; (2) grass-silage; and (3) chopped hay. After 6 months a single oral dose of D-alpha-[5-Me-3H]tocopherol was given to each sheep on these three treatments. 2. Blood plasma, rumen liquor and urine radioactivity were measured for 4 d and, at the end of this period, the animals were killed and tissue distribution of 3H was determined. 3. Maize-silage generally contained less alpha-tocopherol than grass-silage or hay. Tissue uptake of 3H was greater on maize-silage than other diets. 4. In muscle, spleen and liver, tocopherol concentrations were lower in the maize-silage than the grass-silage fed animals. 5. A tendency to higher uptake of radioactivity was recorded at all times in the plasma and its lipid extract of sheep fed on maize-silage than those fed on grass-silage or hay. 6. Urine clearance of radioactivity tended to be higher in animals fed on the maize-silage than those fed on grass-silage or hay. This difference of magnitude in urinary excretion was probably related to the rate of metabolism of the ingested radiotocopherol.
D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004043:Dietary Fiber; D008297:Male; D012417:Rumen / Q000378:metabolism; D012756:Sheep / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism*; D003313:Zea mays
D004043:Dietary Fiber; D014810:Vitamin E