The influence of D-penicillamine on the proliferation rate of cells from the upper limb bud of mouse embryos in vitro.
D-Penicillamine was given on the fourth day of culture of limb buds from 11-day mouse embryos at a dose of 100 microng/ml medium and allowed to react for 24 hours. On day 5 there was found a significant decrease in the [3H] thymidine labeling index of cartilage cells and of cells of the perichondrium, whereas mesenchyme cells showed no decrease in proliferation under these conditions. The toxic effects of D-penicillamine on prenatal skeletal developement in vivo could therefore result not only from direct action on collagen but also via inhibition of proliferation of cartilage cells.
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000196:embryology; D002455:Cell Division / Q000187:drug effects*; D005552:Forelimb / Q000166:cytology / Q000187:drug effects / Q000196:embryology*; D051379:Mice; D009924:Organ Culture Techniques; D010396:Penicillamine / Q000494:pharmacology*; D013997:Time Factors
D010396:Penicillamine