Deviated lysis: transfer of complement lytic activity to unsensitized cells. IV. Parital isolation of the activity.
Deviated lysis (d.l.) was previously characterized as the lysis of non-sensitized erythrocytes by activated complement (C) in the presence of EDTA (1, 2, 3). The lytic activity was present in serum fractions of a m.w. in the proximity of 220,000. All the C factors C5 through C9 were found in these fractions and they were all needed for lysis. It is proposed that in d.l. small aggregates of the C components C5 through C9 coexist in the reaction mixture without further interaction. Only when appropriate receptors such as present on target cells surfaces are available, the factors react in a sequential order eventually to result in lysis of the target cell.
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification / Q000502:physiology; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003182:Complement C5 / Q000378:metabolism; D003183:Complement C6 / Q000378:metabolism; D003184:Complement C7 / Q000378:metabolism; D003185:Complement C8 / Q000378:metabolism; D003186:Complement C9 / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism*; D004586:Electrophoresis; D004912:Erythrocytes / Q000276:immunology*; D006461:Hemolysis; D007444:Inulin / Q000494:pharmacology; D008970:Molecular Weight; D013696:Temperature; D013997:Time Factors
D001798:Blood Proteins; D003182:Complement C5; D003183:Complement C6; D003184:Complement C7; D003185:Complement C8; D003186:Complement C9; D007444:Inulin; D003165:Complement System Proteins