Origin of esterases in human whole saliva.
Whole saliva of 59 healthy persons was used for determination of esterase activity. The pattern of esterase was studied by means of isoelectrofocusing on thin-layer acrylamide gels. The esterases found in whole saliva are suggested to be derived from the cells of the tissue in the oral cavity. This origin is indicated (e.g.) by comparison between isolectrophoretic esterase patterns of whole saliva, submandibular saliva, gingival biopsy and fibroblast culture. Antisera against partially purified saliva esterases were produced in rabbits. These sera, used in immunoelectro-osmophoresis, gave esterase-active immunoprecipitate against whole saliva, the water-soluble materials of disrupted gingival biopsy and fibroblast culture, but not against the material of the bacteria, Streptococcus sanguis, Strptococcus mutans, Streptococcus mitis, Actinomyces viscosus and Lactobacillus fermentum.
D000681:Amylases / Q000378:metabolism; D002478:Cells, Cultured; D004950:Esterases / Q000302:isolation & purification / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007122:Immunoelectrophoresis; D012463:Saliva / Q000201:enzymology*
D004950:Esterases; D000681:Amylases