Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.
A method is described that makes use of a new polyamide resin for the rapid synthesis of short oligodeoxyribonucleotides. The method is illustrated by the preparation of two heptadeoxyribonucleotides, d(pT6-C) and d(pC-A-G-T-G-A-T) using a phosphodiester approach. A further development involved use of phenyl isocyanate as an in situ drying agent, which obviated the need for solvent co-evaporation prior tothe internucleotidic coupling steps. Improved fractionation of thymidyl oligonucleotides was obtained by use of a new microparticulate, silica-based anion-exchanger.
D000577:Amides; D008722:Methods; D009570:Nitriles; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D009841:Oligonucleotides / Q000138:chemical synthesis*; D012116:Resins, Plant
D000577:Amides; D009570:Nitriles; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D012116:Resins, Plant