Oligosaccharides of human milk. Structural studies of two new octasaccharides, difucosyl derivatives of para-lacto-N-hexaose and para-lacto-N-neohexaose.
Two new octasaccharides were isolated from human milk of a nonsecretor Lewis a+b- individual. Structural studies by sequential enzymic degradation and by quantitative methylation analysis revealed that their structures were as follows: Galbeta1 leads to 3 (Fucalpha1 leads to 4) GlcNAcbeta1 leads to 3Galbeta1 leads to 4 (Fucalpha1 leads to 3) GlcNAcbeta1 leads to 3Galbeta1 leads to 4Glc, Galbeta1 leads to 4 (Fucalpha1 leads to 3) GlcNAcbeta1 leads to 3Galbeta1 leads to 4 (Fucalpha1 leads to 3) GlcNAcbeta1 leads to 3Galbeta1 leads to 4Glc. The core portions of these sugars, which are newly found linear hexasaccharides, are named para-lacto-N-hexaose and para-lacto-N-neohexaose, respectively.
D002241:Carbohydrates / Q000032:analysis; D005260:Female; D005643:Fucose / Q000032:analysis; D006801:Humans; D008895:Milk, Human / Q000032:analysis*; D008968:Molecular Conformation; D009844:Oligosaccharides / Q000302:isolation & purification; D011247:Pregnancy
D002241:Carbohydrates; D009844:Oligosaccharides; D005643:Fucose