Specific immunostaining of CCK cells by use of synthetic fragment antisera.
Antibodies to the central fragments 9-20 dodecapeptide sequence of CCK were used for specific immunostaining of the CCK cells of the mammalian gut. The use of high specific antibodies to synthetic fragment, essential when there is a possibility of immunochemical cross reactions between antisera and hormones of similar molecular structure provides the key to increased understanding of the nature and relationships of peptide hormones.
D000918:Antibody Specificity; D002766:Cholecystokinin / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions; D004386:Duodenum / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D005755:Gastrins / Q000276:immunology / Q000378:metabolism; D006728:Hormones / Q000138:chemical synthesis / Q000276:immunology; D007082:Ileum / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology
D005755:Gastrins; D006728:Hormones; D009842:Oligopeptides; D002766:Cholecystokinin