title: The role of RNA metabolism in polymorphonuclear leukocyte phagocytosis.
abstract: The role of RNA metabolism in human PMN phagocytosis was examined. By the inhibition of RNA synthesis with actinomycin D, intracellular killing of a strain of actinomycin D-resistant S. epidermidis was unaffected by 1 hr but significantly decreased by 2 and 3 hr. After 1 hr, phagocytosis began to decrease, and iodination of proteins also decreased in the presence of actinomycin D. Hexosemonophosphate shunt activity remained similar for both actinomycin D treated PMN's and control cells at 30 min and 2 hr. Protein synthesis was inhibited by incubating PMN's with puromycin. The ability of the PMN's to kill puromycin-resistant S. epidermidis was significantly decreased at 2 and 3 hr.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001770:Blood Bactericidal Activity; D003609:Dactinomycin / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005947:Glucose / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007455:Iodine / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D010084:Oxidation-Reduction; D010587:Phagocytosis / Q000187:drug effects; D011691:Puromycin / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis*; D013210:Staphylococcus / Q000187:drug effects / Q000254:growth & development
chemical_list: D001426:Bacterial Proteins; D003609:Dactinomycin; D011691:Puromycin; D012313:RNA; D007455:Iodine; D005947:Glucose