Gamma-radiation-induced chromosomal aberrations in human lymphocytes: dose-rate effects in stimulated and non-stimulated cells.
Stimulated and non-stimulated human peripheral blood lymphocytes were irradiated acutely and chronically, over 24 h. Dose-effect relationships for dicentric chromosomes were established and various models were fitted to the data. At prolonged irradiations the yield decreased in basic agreement with the linear-quadratic model of abberration induction. Dose-protraction experiments of PHA+ and PHA- lymphocytes, irradiated under various conditions of oxygenation and suspension (culture medium, whole blood) showed that the rejoining time increased from about 3 h in non-stimulated cells to about 10 h after PHA stimulation, and that this retarded rejoining was most likely due to blastic transformation itself and not to other conditions of irradiation.
D000328:Adult; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003470:Culture Media; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D010100:Oxygen; D010313:Partial Pressure; D011839:Radiation, Ionizing
D003470:Culture Media; D037102:Lectins; D010100:Oxygen