Pharmacokinetics of methylphenidate in the rat using single-ion monitoring GLC-mass spectrometry.
A GLC-mass spectrometric assay for methylphenidate in biological fluids was developed using the ethyl ester homolog of the drug as the internal standard. The procedure has a lower level of sensitivity of 1.2 ng/ml and is based on GLC-mass spectrometic monitoring of the m/e 180 ion common to the mass spectra of the N-trifluoroacetyl derivatives of the drug and internal standard. The brain and plasma levels of methylphenidate in rats were determined after intravenous administration of 0.5 mg/kg of the drug. The two-compartment open pharmacokinetic model fit the data.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002849:Chromatography, Gas; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry; D008722:Methods; D008774:Methylphenidate / Q000097:blood / Q000378:metabolism*; D008954:Models, Biological; D051381:Rats
D008774:Methylphenidate