L-Histidinol inhibits specifically and reversibly protein and ribosomal RNA synthesis in mouse L cells.
The addition of the histidine analogue L-histidinol to mouse L cells in suspension culture results in an abrupt inhibition of protein and ribosomal RNA synthesis with relatively little effect on messenger RNA production. Messenger RNA synthesized in the presence of L-histidinol associates with ribosomal subunits to form initiation complexes whose ability to commence elongation appears impaired. As a result of this mode of action, L-histidinol treatment results in a dramatic reduction of polyribosomes and a concomitant accumulation of 80 S ribosomes. The inhibition of both protein and ribosomal RNA synthesis mediated by L-histidinol is rapidly reversed by the addition of L-histidine to treated cultures.
D006639:Histidine / Q000494:pharmacology; D006641:Histidinol / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology*; D007700:Kinetics; D007739:L Cells / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis*
D007093:Imidazoles; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D006639:Histidine; D006641:Histidinol