title: Purification and radioimmunoassay of chicken growth hormone.
abstract: Chicken growth hormone has been isolated from adenohypophysial tissue from which the glycoprotein hormones had been removed. The procedure entailed alkali extraction, ammonium sulphate precipitation and ion-exchange chromatography on DEAE-cellulose. The resulting fraction was homogeneous, active in the rat tibia bioassay and had a similar isoelectric point, molecular weight and amino acid composition to mammalian growth hormone. A specific homologous radioimmunoassay has been developed using the avian growth hormone.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D013006:Growth Hormone / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D010903:Pituitary Gland, Anterior / Q000032:analysis; D011863:Radioimmunoassay
chemical_list: D013006:Growth Hormone