A simple method for the preparation of homogeneous phospholipid vesicles.
A new method is described for the preparation of homogeneously sized, single-lamellar phospholipid vesicles. This method, wich is based on differential high-speed ultracentrifugation, has the advantages of a higher vesicle yield without dilution and rapidity of preparation when compared to the molecular-sieve technique. The homogeneity of vesicle dispersions, prepared by this new method, is examined by several physical techniques and found to be comparable to the best samples prepared by molecular-sieve chromatography.
D004058:Diffusion; D008567:Membranes, Artificial; D008722:Methods; D008954:Models, Biological; D008968:Molecular Conformation; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013109:Sphingomyelins
D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013109:Sphingomyelins