Improved method and its clinical application of a radioimmunoassay of arginine vasopressin in human serum.
A sensitive and specific double-antibody radioimmunoassay for measuring circulating levels of arginine vasopressin in human serum is described. It is possible to detect arginine vasopressin levels of 1 microU/ml serum without extraction procedure. Normal subjects were found to have 5.7 +/- 4.4 microU/ml after a dehydration period of 12 hours. Water loading diminished arginine vasopressin concentrations while dehydration increased it. Application of furosemide over a period of 14 days brought forth constant but not significant decreases. Subjects suffering from psychogenic polydipsia showed normal levels in spite of drinking 8-12 liters of water per day. Patients suffering from liver cirrhosis with ascites showed significantly higher arginine vasopressin levels, approaching normal values, when ascites was under control.
D001127:Arginine Vasopressin / Q000097:blood*; D003919:Diabetes Insipidus / Q000097:blood; D005069:Evaluation Studies as Topic; D005665:Furosemide; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood; D008236:Lypressin / Q000097:blood; D009994:Osmolar Concentration; D010121:Oxytocin / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods; D013894:Thirst; D014667:Vasopressins / Q000031:analogs & derivatives*
D014667:Vasopressins; D001127:Arginine Vasopressin; D010121:Oxytocin; D008236:Lypressin; D005665:Furosemide