Temperature effects on lymphocyte transformation invitro.
Phytohemagglutinin (PHA)-induced transformation of normal rat peripheral lymphocytes has been studied at a wide range of culture temperatures (4 degrees C to 42 degrees C). Lymphocyte transformation was maximum at 37 degrees C while insignificant stimulation was observed between 4 degrees C and 30 degrees C. Temperatures above 37 degrees C produced sub=optimal transformation as measured by synthesis of DNA and protein, and appearance of lymphoblasts. Binding studies using 125I-PHA indicate that the low temperature inhibition of lymphocyte transformation could be a result of excess lectin (being available as a result of low temperature) bound to the cell surface, preventing the initiation of the molecular events associated with transformation.
D002462:Cell Membrane; D003080:Cold Temperature; D006358:Hot Temperature; D037102:Lectins; D008213:Lymphocyte Activation; D008214:Lymphocytes; D013997:Time Factors
D037102:Lectins