A modified push-pull system for the localised perfusion of brain tissue.
A method for monitoring the outflow of a push-pull perfusion system is presented. This modification possesses the following important advantages: (1) Inadvertent induction of expansion lesions is avoided, (2) Flow rates can be reduced from the usual 50-200 microliter/min to 0.6-1.7 microliter without decreasing the probability of detecting occlusions, (3) Incidence of occlusions is reduced, (4) Up to 30--40 perfusions can be performed at each of several sites in the brain of a single animal over a period of several months. The modified system has been used successfully to administer neurohumoral agents to the brain of rats and to determine the release of endogenous substances into the perfusate.
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000502:physiology*; D010477:Perfusion / Q000295:instrumentation* / Q000379:methods; D051381:Rats