title: Hemoglobinometry: A comparison between the hemiglobincyanide method and the Coulter S counter.
abstract: The relative accuracy and precision of the Coulter S Counter have been evaluated in comparison with manual hemiglobincyanide determination according to the recommendations of the International Committee for Standardization in Hematology. Some improvements in the manual procedure, such as centrifugation of hemiglobincyanide solutions and the use of the detergent Triton X-100, were also tested. The Coulter S Counter generally gives higher precision in comparison with the manual method. Nevertheles, Coulter S determinations are systematically lower due to both constant and proportional errors. The available data ranged between hemoglobin values of 11.5 and 18.5%, giving differences of 0-8% between hemoglobin values determined by the Coulter S Counter and the hemiglobincyanide method.
mesh_terms: D003902:Detergents; D006452:Hemoglobinometry / Q000295:instrumentation / Q000379:methods*; D006454:Hemoglobins; D006801:Humans; D008027:Light; D009570:Nitriles; D013053:Spectrophotometry
chemical_list: D003902:Detergents; D006454:Hemoglobins; D009570:Nitriles