title: Attempts to purify hemopoietic stem cell enrichment in bone marrow by use of glass wool filtration.
abstract: This study is about enrichment of hemopoietic stem cell concentration in mouse bone marrow by use of a glass wool filtration technique. Maximum stem cell concentration was accomplished when filtration conditions were as follows: (1) support medium supplemented to 15% with fresh serum from syngeneic donors; (2) no incubation time allowed for cells on the columns; and (3) an operational temperature of 24 degrees C. With these conditions, stem cell concentrations were increased 5,4-fold relative to the concentration in untreated marrow.
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002999:Clone Cells; D005374:Filtration / Q000379:methods*; D006412:Hematopoietic Stem Cells; D051379:Mice; D013048:Specimen Handling