An improved method for the preparation of the DNA-dependent RNA polymerase B from calf thymus.
A simplified method is described for the large-scale preparation of a highly purified DNA-dependent RNA polymerase B from calf thymus. The method includes homogenization and lysis of the tissue, chromatography on DEAE-cellulose, phosphocellulose, hydroxylapatite-Sephadex G-10 and, once again, phosphocellulose. The procedure avoids the preparation of nuclei, the use of sonication, ammonium sulphate precipitations and dialysis steps and needs no ultracentrifugation.
D000818:Animals; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification*; D012319:RNA Polymerase II / Q000302:isolation & purification*; D013950:Thymus Gland / Q000201:enzymology*
D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases