A simple method for purification of epoxide hydratase from rat liver.
Rat liver epoxide hydratase was purified 460-fold to homogeneity by detergent solubilization and ion-exchange chromatography. The enzyme obtained in high yield (36%) exhibited a specific activity of 479nmol of styrene glycol formed/min per mg of protein, with styrene oxide as substrate. Only one polypeptide-staining band, mol.wt. 49500, was visible after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.
D000818:Animals; D002852:Chromatography, Ion Exchange; D003902:Detergents; D004589:Electrophoresis, Disc; D004851:Epoxide Hydrolases / Q000302:isolation & purification*; D006836:Hydro-Lyases / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology*; D008722:Methods; D051381:Rats; D012995:Solubility
D003902:Detergents; D004851:Epoxide Hydrolases; D006836:Hydro-Lyases