[Structural and functional integration of the processes of membrane hydrolysis and transport ("permeome" hypothesis)].
It was suggested and experimentally proved that the transport system's input is a part of an enzyme-transport ensemble and an allosteric regulator of enzyme activity. In special experiments, a typical for cooperation sygmoidal kinetics of saccharose and maltose hydrolysis was shown, as well as of their hydrolysis products transport, if the enzyme is involved in the actively transporting intestine mucosa. On the contrary, it is the hyperbolic curve which is specific for isolated digestive enzymes and for glucose accumulation. Replacement of oxygen with nitrogen and specific blockage of the glucose transporting agent with floridzine leads to disappearance or sharp reducing of the sygmoidality. The term "ergome" is suggested for designation of the minimal ensemble of elements able to carry out an elementary physiological process, the term "permeome"--for the transporting ergome.
D000494:Allosteric Regulation; D000681:Amylases / Q000378:metabolism; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism*; D002463:Cell Membrane Permeability; D005696:Galactosidases / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000201:enzymology / Q000378:metabolism*; D007700:Kinetics; D009844:Oligosaccharides / Q000378:metabolism*; D013393:Sucrase / Q000378:metabolism
D009844:Oligosaccharides; D000681:Amylases; D005696:Galactosidases; D013393:Sucrase; D005947:Glucose
Strukturnaia i funktsional'naia integratsiia protsessov membrannogo gidroliza i transporta (gipoteza "permeoma")