title: Concanavalin A binding to the epithelial surface of the developing mouse olfactory placode.
abstract: Concanavalin A (Con A), a lectin binding to mannosyl and glucosyl residues of glycoproteins and glycolipids, was used to study the appearance of carbohydrate-rich cell surface material on the olfactory placode and nasal processes which contribute to formidine was also used in an attempt to correlate changes in labeling index with formation of the olfactory placode and nasal processes. The cell surface of the early frontonasal epithelium binds Con A very little, if at all, but Con A binding was observed when the olfactory placode could be identified as a plate of cuboidal cells that exhibited a reduced labeling index. During the period of formation of the nasal processes, Con A binding was observed on the facial epithelium including the presumptive contact region. There was also a decline in the labeling index throughout primary palate formation. This study provides three criteria by which the olfactory placode can be identified: a morphological change of placode cells to a cuboidal shape, a synthesis or rearrangement of surface coat material that binds Con A, and a reduced labeling index.
mesh_terms: D000818:Animals; D003208:Concanavalin A / Q000032:analysis; D004475:Ectoderm / Q000166:cytology / Q000378:metabolism; D051379:Mice; D009297:Nasal Mucosa / Q000196:embryology; D009666:Nose / Q000196:embryology*; D009831:Olfactory Mucosa / Q000196:embryology; D009832:Olfactory Nerve / Q000196:embryology; D010159:Palate / Q000196:embryology*; D011952:Receptors, Concanavalin A / Q000032:analysis*; D011955:Receptors, Drug / Q000032:analysis*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D014316:Tritium; D003208:Concanavalin A; D013936:Thymidine