The isolation of polyribosomes from plant material using magnesium precipitation in the presence of heparin.
A procedure is described for the isolation of polyribosomes from crude tissue homogenates of germinated pea seeds using Mg2+ precipitation in the presence of the ribonuclease inhibitor heparin. Collection of the polyribosomal pellet does not require the use of an ultracentrifuge. The method has a potentially wide application for polyribosome and messenger ribonucleoprotein isolation and might also be useful where conventional techniques have failed.
D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient; D006493:Heparin; D008274:Magnesium; D010944:Plants / Q000648:ultrastructure*; D011132:Polyribosomes / Q000648:ultrastructure*; D014461:Ultracentrifugation
D006493:Heparin; D008274:Magnesium