Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes.
Total cytoplasmic polyadenylated RNA was isolated from the human lymphocyte cell line Wil2 by oligo(dT)-cellulose chromatography. Tritiated complementary DNA (cDNA) was transcribed from the RNA and used as a probe for in situ hybridization to metaphase chromosomes. The majority of G-negative or lightly staining bands were found to be preferential sites of hybridization.
D002460:Cell Line; D002874:Chromosome Mapping; D004247:DNA; D006801:Humans; D008214:Lymphocytes; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger; D013154:Spleen; D014158:Transcription, Genetic
D012333:RNA, Messenger; D004247:DNA