Purification of beta-N-acetyl-D-glucosaminidase of the horseshoe crab by affinity chromatography.
An affinity column has been synthesized consisting of p-aminophenyl 1-thio-beta-L-fucopyranoside residues attached to Sepharose 4B through succinylated diaminodipropylamine bridges. Surprisingly, it has been found to bind beta-N-acetylglucosaminidase in the serum of Limulus polyphemus (horseshoe crab). The enzyme is eluted with N-acetyl-D-glucosamine at a concentration of 2 mg/ml and with other sugars at higher concentrations. A highly purified enzyme free from other glycosidases is obtained. The enzyme is not eluted by solutions of salt.
D000118:Acetylglucosaminidase / Q000097:blood*; D000818:Animals; D002846:Chromatography, Affinity; D006596:Hexosaminidases / Q000097:blood*; D006737:Horseshoe Crabs; D008722:Methods
D006596:Hexosaminidases; D000118:Acetylglucosaminidase