Determination of plasma glucose with use of a stirrer containing immobilized glucose dehydrogenase.
A stirrer containing immobilized glucose dehydrogenase has been successfully used for determining glucose in plasma. The device is usable for at least two months and for about 500 assays. The reaction was measured kinetically and linearity was observed to 4 g of glucose per liter. Tested with aqueous glucose and with deproteinized plasma, within-day and day-to-day precision were good. Interference and method-comparison (hexokinase method) were examined. The performance of this system makes the technique useful and attractive for routine use in small-volume clinical laboratories.
D001786:Blood Glucose / Q000032:analysis*; D001798:Blood Proteins / Q000302:isolation & purification; D002238:Carbohydrate Epimerases; D002624:Chemistry, Clinical / Q000295:instrumentation*; D004355:Drug Stability; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D006801:Humans; D008722:Methods; D013997:Time Factors
D001786:Blood Glucose; D001798:Blood Proteins; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D002238:Carbohydrate Epimerases