Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.
The methylation pattern of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus has been determined. Analysis of purified high-molecular-weight RNA synthesized with S-[methyl-3H]-adenosylmethionine and alpha[32P]UTP as precursors gave the following results. (i) Eessentially all molecules contained blocked and methylated structures of the type m7G(5')ppp(5')Gm and m7G(5')ppp(5')Am. (ii) There was no detectable methylation at internal sites. (iii) Under several different conditions of synthesis, the ratio of molecules containing m7G(5')ppp(5')Gm to those containing m7G(5')ppp(5')Am was imilar for both the virion-associated high-molecular-weight RNA and the virion-released 8-12S mRNA.
D001483:Base Sequence; D002474:Cell-Free System; D006367:HeLa Cells; D008745:Methylation; D008970:Molecular Weight; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D012436:S-Adenosylmethionine / Q000378:metabolism; D014500:Uracil Nucleotides / Q000378:metabolism; D014616:Vaccinia virus / Q000378:metabolism*
D012333:RNA, Messenger; D012367:RNA, Viral; D014500:Uracil Nucleotides; D012436:S-Adenosylmethionine