A paper chromatoelectrophoresis method for the determination of bilirubin binding to serum protein.
A technique is described in which paper chromatoelectrophoresis is used to measure the bilirubin binding capacity of albumin in serum. It is equivalent to the Sephadex G-25 method and its results are highly reproducible. From a single pool of serum, multiple determinations indicated the mean bilirubin binding capacity to be 23.7 +/- 0.76 (SD) mg/dl and the greatest difference between any two values was 2 mg/dl. This new technique can determine the bilirubin binding capacity of a serum sample in 15 min. Highly skilled technicians are not needed and very small quantities of serum are required, which gives it a definite advantage over the Sephadex G-25 method.
D001663:Bilirubin / Q000097:blood*; D002854:Chromatography, Paper; D004590:Electrophoresis, Paper; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000097:blood; D007234:Infant, Premature; D007567:Jaundice, Neonatal / Q000097:blood / Q000175:diagnosis; D009401:Nephrosis / Q000097:blood; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*
D012709:Serum Albumin; D001663:Bilirubin