[Histochemical and ultrastructural investigations on the activity of methylglyoxal (bis)-guanylhydrozan (methyl-GAG) on organ cultures of malignant tumors (author's transl)].
Methyl-GAG was tested in organotypic cultures of malignant tumors of human and mice. In 3 cases, a reduction of the activity of two oxydoreductases (lactate dehydrogenase and NADH-diaphorase) after treatment with methyl-GAG was observed whereas in 19 other cultivated tumors no change of enzyme activity was induced by methyl-GAG. Electronmicroscopy revealed only minor structural alterations of tumor cells after application of methyl-GAG as compared with control cultures.
D000818:Animals; D001943:Breast Neoplasms / Q000201:enzymology / Q000648:ultrastructure*; D002478:Cells, Cultured / Q000187:drug effects; D005260:Female; D006146:Guanidines / Q000494:pharmacology*; D006651:Histocytochemistry; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology / Q000648:ultrastructure; D008545:Melanoma / Q000201:enzymology / Q000648:ultrastructure; D051379:Mice; D008935:Mitoguazone / Q000494:pharmacology*; D009252:NADPH Dehydrogenase / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000201:enzymology / Q000648:ultrastructure*
D006146:Guanidines; D007770:L-Lactate Dehydrogenase; D009252:NADPH Dehydrogenase; D008935:Mitoguazone
Histochemische und ultrastrukturelle Untersuchungen über den Einfluss von Methylglyoxal (bis)guanylhydrazon (Methyl-GAG) auf Organkulturen von malignen Tumoren