Single inoculation immune hamster sera for typing California group arboviruses by the complement-fixation test.
Eight reference California group viruses of North America were typed using sera of immune hamsters bled 21 days after a single inoculation. The complement-fixation test reactions were relatively specific, although only 2-fold differences were observed reciprocally with the closely-related La Crosse and snowshoe hare viruses. Hamster serum taken 10 days post inoculation was more specific than a 21-day serum. Sepcificity after second inoculation was lost with some antigens. Jamestown Canyon and South River viruses were identical by complement-fixation test and showed minor differences in the plaque reduction neutralization test.
D000818:Animals; D003168:Complement Fixation Tests; D006224:Cricetinae; D003429:Cross Reactions; D002141:Encephalitis Virus, California / Q000302:isolation & purification*; D004668:Encephalitis Viruses / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D007106:Immune Sera; D009500:Neutralization Tests; D012703:Serotyping / Q000379:methods*
D007106:Immune Sera