[Heterogeneity of carbohydrate chains of acidic bronchial mucin isolated from the spatum of two subjects with chronic bronchitis].
Acidic glycoproteins having blood group H activity were isolated from the sputum of two patients suffering from chronic bronchitis by reduction of the fibrillar mucus, chromatography on ECTEOLA-cellulose and gel filtration on Sepharose 4-B. These glycoproteins were degraded with alkaline borohydride and the degradation products were fractionated by chromatography on ionexchange resins and by gel filtration. The carbohydrate chains have a wide heterogeneity with regard to acidity and molecular size. Therefore carbohydrate chain heterogeneity which was already observed for bronchial glycoproteins isolated from a patient suffering from cystic fibrosis is not specific for cystic fibrosis.
D000596:Amino Acids / Q000032:analysis; D001991:Bronchitis / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002908:Chronic Disease; D006801:Humans; D009077:Mucins / Q000032:analysis*; D013183:Sputum / Q000032:analysis*; D013431:Sulfates / Q000032:analysis
D000596:Amino Acids; D002241:Carbohydrates; D009077:Mucins; D013431:Sulfates
Heterogeneite des chaines glycanniques des mucines bronchiques acides isolees a partir de l'expectoration de deux sujets atteints de bronchite chronique.