Multiple chromatographic peaks of phenylalanyl-tRNA associated with spontaneous hydrolysis of Y base during isolation.
In contrast to the single phenylalanyl-tRNA found in normal cells, some tumours are known to have more than one phenylalanine isoacceptor. However, during certain steps in tRNA isolation from normal or tumour tissue, additional chromatographic peaks can be artificially produced which may be confused with the tumour-specific Phe-tRNA. Such procedures as extraction with unbuffered phenol and unbuffered gel filtration chromatography appear to produce adventitious isoacceptors by hydrolysis of Y base.
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002850:Chromatography, Gel; D008099:Liver; D010636:Phenols; D010649:Phenylalanine; D012343:RNA, Transfer / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats
D010636:Phenols; D010649:Phenylalanine; D012343:RNA, Transfer