Reactions of gold(III) ions with ribonuclease A and methionine derivatives in aqueous solution.
1. Addition of Au(III) (as chloroaurate) to folded ribonuclease A causes the formation of aggregates, as shown by chromatography and extreme broadening of the 270 MHZ 1H NMR spectrum. 2. When RNAase is partially unfolded Au(III) causes oxidation of methionines to the sulphoxide, and leads to almost complete unfolding (at molar equivalent ratios). 3. Reactions between model methionine derivatives and Au(III) show that the oxidation involves the production of Au(I)-methionine species. The stability of these complexes is dependent on the availability of free NH2 groups which catalyse their disproportionation.
D006046:Gold / Q000494:pharmacology; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008715:Methionine / Q000031:analogs & derivatives*; D011487:Protein Conformation; D012260:Ribonucleases / Q000378:metabolism
D046911:Macromolecular Substances; D006046:Gold; D008715:Methionine; D012260:Ribonucleases