Characterization of C-4-methylated sterols by pyridine-induced solvent shifts in proton magnetic resonance spectroscopy.
The proton magnetic resonance (PMR) spectra were measured in deuterochloroform (CDC13) and pyridine solutions for some 4-desmethyl, 4alpha-methyl, 4beta-methyl, and 4,4-dimethyl sterols related to 5alpha-cholestane series as well as for their C-3-oxo derivatives. The influence of pyridine, relative to CDC13, on methyl group chemical shifts was discusssed.  The technique utilizing pyridine-induced solvent shifts in PMR spectroscopy was found useful in characterizing the individual classes of sterols.
D002725:Chloroform; D009682:Magnetic Resonance Spectroscopy; D011522:Protons; D011725:Pyridines; D013261:Sterols
D011522:Protons; D011725:Pyridines; D013261:Sterols; D002725:Chloroform