Influence of glucose and inhibitors of glycolysis on release of total proteins and enzymes from human leukocytes.
We studied the influence of substrates and glycolysis inhibitors on the release of proteins and enzymes from human leukocytes. We show the relationship between metabolic level and percentage of enzyme release. Leukocyte kept alive in a nutrient-free bicarbonate medium liberate enzymes even though their metabolic state is satisfactory. The addition of glucose to themedium significantly decreases this phenomenon without affecting the energy level. Addition of glycolysis inhibitors increases it, reduces the energy level, and interferes with other metabolic pathways. The results are discussed and compared to those obtained by other authors using various cell models.
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology; D003594:Cytoplasmic Granules / Q000201:enzymology; D003864:Depression, Chemical; D004798:Enzymes / Q000097:blood*; D005947:Glucose / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007461:Iodoacetates / Q000494:pharmacology; D007962:Leukocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology
D001786:Blood Glucose; D001798:Blood Proteins; D004798:Enzymes; D007461:Iodoacetates; D012969:Sodium Fluoride; D005947:Glucose