Preparation and properties of trypsin and chymotrysin coupled covalently to poly (N-vinylpyrrolidone).
Water-soluble poly(N-vinylpyrrolidone) of molecular weight 10 000 was modified by hydrolysis of 5% of the gamma-lactam rings, and formation of the N-hydroxysuccinimidester. This activated polymer was bound covalently to trypsin (EC 3.4.21.4) and chymotrypsin (EC 3.4.21.1) to complexes of a molecular weight of about 150 000. The bound enzymes showed an increase in stability towards autolysis. Towards small molecular weight substrates the trypsin-poly (N-vinylpyrrolidone) conjugate showed an increase in specific activity of 1: 1.6, whereas the activity towards larger substrate was found to be only 20%. Chymotrypsin-poly(N-vinylpyrrolidone) showed decreased activity against small (50%) and large (7%) molecular weight substrates.
D055598:Chemical Phenomena; D002621:Chemistry; D002918:Chymotrypsin / Q000378:metabolism; D002919:Chymotrypsinogen; D004355:Drug Stability; D008970:Molecular Weight; D011205:Povidone; D011485:Protein Binding; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors
D014361:Trypsin Inhibitors; D002919:Chymotrypsinogen; D002918:Chymotrypsin; D014357:Trypsin; D011205:Povidone