title: A rapid sensitive radioassay for serum native and denatured DNA.
abstract: A radioassay for human serum DNA using 3H-actinomycin D was developed and evaluated. The assay was found to be technically simple, precise, sensitive and specific for DNA. Both native and denatured DNA levels could be measured using a nuclease specific for sDNA, since denatured DNA reproducibly bound 3H-actinomycin D under the assay conditions. Analysis of human sera yielded results in accord with those previously reported for man.
mesh_terms: D004247:DNA / Q000097:blood*; D003609:Dactinomycin; D006801:Humans; D008722:Methods; D009691:Nucleic Acid Denaturation; D014316:Tritium
chemical_list: D014316:Tritium; D003609:Dactinomycin; D004247:DNA