Dog liver 3-hydroxy-3-methylglutaryl coenzyme A reductase.
A rapid and sensitive assay method for 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-coA reductase, EC 1.1.1.34) is described. HMG-coA reductase is demonstrated in dog liver microsomes, and the converted reaction product has been identified as mevalonolactone. The enzyme activity undergoes cyclic variation and increases by more than tenfold 5 h after feeding. The properties of dog liver enzyme are generally similar to the rat liver enzyme in the method of solubilization, cold inactivation, pH optimum, and Km values.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D004285:Dogs; D004435:Eating; D005260:Female; D006903:Hydroxymethylglutaryl CoA Reductases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008722:Methods; D008862:Microsomes, Liver / Q000201:enzymology*; D012995:Solubility
D000429:Alcohol Oxidoreductases; D006903:Hydroxymethylglutaryl CoA Reductases