A simplified technique for in vivo analysis of sister-chromatid exchanges using 5-bromodeoxyuridine tablets.
A small tablet of 5-bromodeoxyuridine (BrdU), implanted subcutaneously in a mouse, provides sustained release of base analog sufficient to effect substitution of DNA throughout an entire replication period. As illustrated by studies of mouse bone-marrow and spleen cells in the presence or absence of cyclophosphamide, this depot method of BrdU administration greatly simplifies in vivo analysis of sister-chromatid-exchange formation.
D000818:Animals; D001853:Bone Marrow / Q000648:ultrastructure; D001854:Bone Marrow Cells; D001973:Bromodeoxyuridine; D002455:Cell Division; D002842:Chromatids; D003692:Delayed-Action Preparations; D004343:Drug Implants; D008722:Methods; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000648:ultrastructure
D003692:Delayed-Action Preparations; D004343:Drug Implants; D001973:Bromodeoxyuridine