Isolation and characterization in vitro of an allergen from plane-tree (Platanus acerifolia) pollen.
A plane-tree pollen allergen was obtained by ion exchange and gel filtration chromatography. It is a glycoprotein with a molecular weight of 22,000. By isoelectrofocusing, two isomers with a high cross-allergenicity were obtained. The results suggest that this fraction is not the only allergen but certainly the most active.
D000485:Allergens / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006801:Humans; D006868:Hydrolysis; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010936:Plant Extracts / Q000032:analysis; D011058:Pollen; D014197:Trees
D000485:Allergens; D010936:Plant Extracts