Histamine and acute haemorrhagic lesions in rat gastric mucosa: prevention of stress ulcer formation by (+)-catechin, an inhibitor of specific histidine decarboxylase in vitro.
Acute haemorrhagic lesions in the oesophagus, stomach and duodenum ('stress ulcers') occur relatively often under clinical conditions and are always dangerous to the patient (lethality rate about 70%). Since conservative and surgical treatment are without significant success up to now, prevention by adaptation to stressors or by administration of drugs seems mandatory. An improved technique for producing acute gastric lesions in rats by immobilization and a new method for assessing this disease in the animals is presented in this communication. High precision is obtained within a single experimental series especially from day to day. Since histamine was suggested to be involved in the pathogenesis of stress ulcer disease, (+)-catechin, a rather specific inhibitor of specific histidine decarboxylase from rat stomach, was tested in immobilized rats. It prevented the formation of acute gastric lesions by 80% in seven series of experiments lasting for half a year. Since the drug has low toxicity in man, it is recommended for clinical trials.
D000704:Analysis of Variance; D000818:Animals; D001578:Benzopyrans / Q000627:therapeutic use*; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D002392:Catechin / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism*; D006640:Histidine Decarboxylase / Q000037:antagonists & inhibitors*; D010438:Peptic Ulcer Hemorrhage / Q000378:metabolism*; D051381:Rats; D012149:Restraint, Physical; D013276:Stomach Ulcer / Q000150:complications / Q000378:metabolism / Q000517:prevention & control*
D001578:Benzopyrans; D006632:Histamine; D002392:Catechin; D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase