title: The intracellular reserve polysaccharide of Clostridium pasteurianum.
abstract: An amylopectinlike polysaccharide (granulose) was the only glucan produced in significant quantities by six wild-type strains of Clostridium pasteurianum grown in glucose minimal medium. The intracellular polysaccharide granules laid down before sporulation contained only this amylopectin. No intracellular dextran was discovered in these wild-type strains, nor in a granulose-negative mutant strain of C. pasteurianum possessing an ADP glucose pyrophosphorylase (EC2.7.7.27) but lacking a granulose synthase (i.e. ADP glucose-alpha-1,4-glucan glucosyl transferase, EC2.4.1.21). Furthermore, methylation analysis demonstrated that (1 leads to 6) linked alpha-D-glucose units accounted for less than 2% of the entire glucose content of these organisms.
mesh_terms: D000687:Amylopectin / Q000032:analysis / Q000096:biosynthesis*; D000693:Anaerobiosis; D002473:Cell Wall / Q000032:analysis; D003013:Clostridium / Q000032:analysis / Q000201:enzymology / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000032:analysis; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D009154:Mutation; D009713:Nucleotidyltransferases / Q000096:biosynthesis; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D012988:Soil Microbiology; D013045:Species Specificity; D013214:Starch Synthase / Q000096:biosynthesis
chemical_list: D011135:Polysaccharides, Bacterial; D000687:Amylopectin; D013214:Starch Synthase; D009713:Nucleotidyltransferases; D005947:Glucose