Digestion of chromatin with deoxyribonuclease II.
The action of DNAse II on DNA in chromatin was studied. The formation of acid-soluble products followed a two-phase kinetic curve. At the end of the first more rapid phase about 25% of DNA was degraded. Early in the degradation process DNA was converted into double stranded fragments, whose sizes were multiples of about 180 base pairs. As the degradation proceeded these fragments were reduced in size. After denaturation DNA from digested chromatin was resolved into discrete single stranded fractions, exact multiples of a ten-nucleotide length, forming a pattern very similar to that observed with DNAase I.chkova LV
D002843:Chromatin; D004273:DNA, Neoplasm; D003851:Deoxyribonucleases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008836:Micrococcal Nuclease / Q000378:metabolism; D008970:Molecular Weight; D009374:Neoplasms, Experimental; D009838:Oligodeoxyribonucleotides / Q000032:analysis
D002843:Chromatin; D004273:DNA, Neoplasm; D009838:Oligodeoxyribonucleotides; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease