title: "Luminal nutrition" versus "functional work-load" as controllers of mucosal morphology and epithelial replacement in the rat small intestine.
abstract: Isotonic solutions of substrate were infused into surgically prepared sacs of upper small intestine in conscious unrestrained rats which fed normally via the gut-in-continuity. Infusion of glucose, galactose or sodium chloride all caused a local increase in villus height, while alpha-methyl glucoside and mannose had no effect. Glucose, galactose and alpha-methyl glucoside all stimulated a similar increase in counts of vincristine metaphases in microdissected crypts. although the absolute level of cell production was lower throughout the gut in glucose-infused rats. Sodium chloride infusion also caused a small stimulation of cell production locally, but a considerable increase in the uninfused gut-in-continuity. Mannose infusion had a variable effect on cell production. These results do not support "luminal nutrition" as a controller of epithelial replacement in the rat small intestine, and suggest that a mechanism based on the work-load of the epithelial may be more appropriate.
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005690:Galactose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000166:cytology* / Q000187:drug effects; D007421:Intestine, Small / Q000166:cytology* / Q000187:drug effects; D008297:Male; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D014750:Vincristine / Q000494:pharmacology
chemical_list: D005960:Glucosides; D012965:Sodium Chloride; D014750:Vincristine; D005947:Glucose; D005690:Galactose