[Sensitive method for determination of proteolytic activity].
The method is developed for estimating the proteolytic activity on the basis of photometric determination at 350 nm of the complexes of products of enzymic splitting of proteins with chloranyl. The advantages of the method are as follows: its high sensitivity (possibility to determine the amount of the enzymic proteolysis products formed from 2-4 microgram of protein), stability of the complexes (three days), wide ranges for direct dependence of absorption on the proteolysis products concentration (0.08-1.2 scale units), simplicity and possibility to conduct determination in the presence of nucleic, acids components.
D055598:Chemical Phenomena; D002621:Chemistry; D002703:Chloranil; D005069:Evaluation Studies as Topic; D009696:Nucleic Acids; D010447:Peptide Hydrolases / Q000032:analysis*; D010783:Photometry; D011809:Quinones
D009696:Nucleic Acids; D011809:Quinones; D002703:Chloranil; D010447:Peptide Hydrolases
Chutliviĭ metod viznachennia proteolitichnoï aktivnosti.