The action of cysteine on PHA-stimulation of lymphocytes. I. Influence of DNA synthesis.
The influence of short-time preincubation of rat lymphocytes in solutions of L-cysteine reduced glutathione, and dithiothreitol on PHA stimulation of these cells was studied by measuring incorporation of 3H-thymidine. Thirty-minute preincubation in cysteine solution enhanced PHA-induced incorporation of 3H-thymidine. One-hour incubation caused earlier peak of incorporation by one day; and two-hour incubation distinctly inhibited spontaneous incorporation of 3H-thymidine. Incubation in solutions of glutathione and dithiothreitol had no effect on the course or intensity of 3H-thymidine incorporation. However, constant presence of these compounds in cultures was toxic for lymphocytes.
D000818:Animals; D002470:Cell Survival; D003545:Cysteine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D004229:Dithiothreitol; D005978:Glutathione; D008213:Lymphocyte Activation / Q000187:drug effects*; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D005978:Glutathione; D003545:Cysteine; D004229:Dithiothreitol; D013936:Thymidine