Structural analysis of the surface polysaccharide of Staphylococcus aureus M.
The chemical structure of the surface polysaccharide from Staphylococcus aureus M was investigated by a combination of methanolytic, hydrolytic, and chromatographic techniques. The repeating unit that was most consistent with the data was a hexasaccharide composed of N-acetyl-D-aminogalacturonic acid, N-acetyl-D-fucosamine, and taurine in molar ratios of 4:2:1. A disaccharide was isolated and characterized, by combined gas-liquid chromatography-mass spectrometry, as N-acetyl-D-aminogalacturonyl-(1 leads to 3)-N-acetyl-D-fucosamine. Taurine is linked to a carboxyl group of N-acetyl-D-aminogalacturonic acid via an amide bond.
D000596:Amino Acids / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D013211:Staphylococcus aureus / Q000032:analysis* / Q000648:ultrastructure; D013654:Taurine / Q000032:analysis
D000596:Amino Acids; D011135:Polysaccharides, Bacterial; D013654:Taurine