The effect of cycloheximide on the replication of measles virus.
Treatment of measles virus-infected cells with cycloheximide results in a three-fold increase of 3H-uridine incorporation into the 12 to 36S mRNA species and in the inhibition of genomic 50S RNA synthesis. Consistent with these observations was the finding of a build-up of polyribosomes but an absence of nucleocapsids in the infected cells. These results suggest that measles virus RNA replication, but not transcription, is dependent upon active protein synthesis.
D002213:Capsid / Q000096:biosynthesis; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology; D008459:Measles virus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D014779:Virus Replication / Q000187:drug effects*
D012333:RNA, Messenger; D012367:RNA, Viral; D003609:Dactinomycin; D003513:Cycloheximide