Synthesis of cartilage-specific proteoglycan by suspension cultures of adult chondrocytes.
Chondrocytes isolated from larynges of adult pigs were cultured as cell suspensions for at least 4 days before use. During continuous-labelling experiments in nutrient medium for 18h with 35SO42- and [3H]glucosamine as precursors, some macromolecular polyanionic material was synthesized which behaved on gel chromatography as proteoglycan. Gel chromatography on Sepharose 2B showed that a proportion of the proteoglycans in the medium appeared to be aggregated, and was dissociated in 4M-guanidinium chloride. Moreover the dissociated proteoglycan interacted with hyaluronic acid. Newly synthesized proteoglycan was larger than the average total cetylpyridinium chloride-precipitable material assayed as uronic acid alone.
D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005944:Glucosamine / Q000378:metabolism; D007817:Laryngeal Cartilages / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis*; D013431:Sulfates / Q000378:metabolism; D013552:Swine; D014574:Uronic Acids / Q000032:analysis
D011509:Proteoglycans; D013431:Sulfates; D014574:Uronic Acids; D005944:Glucosamine