DNA polymerase gamma of rabbit intestinal epithelial cells.
1. DNA-directed DNA polymerase gamma was isolated from epithelial cells of the rabbit small intestine, and characterized. 2. The molecular weight of the enzyme, determined by Sephadex G-200 filtration was 105 000 +/- 15%. 3. The enzyme showed preference for poly(A) replication on poly(A)-poly(dT) or poly(A)-oligo(dT) 12-18 templates, as compared with activated DNA. Poly(C)-oligo(dG)12-18 replication was not observed. 4. The Michaelis constants for dTTP in replication of activated DNA and poly(A)-oligo(dT)12-18 were 3.3 and 2.0 micron, respectively.
D000818:Animals; D002455:Cell Division; D002852:Chromatography, Ion Exchange; D004258:DNA Polymerase III / Q000302:isolation & purification / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D007421:Intestine, Small / Q000166:cytology / Q000201:enzymology*; D008970:Molecular Weight; D011817:Rabbits
D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase