The antigens of pigeon breeder's disease. II. Isolation and characterization of antigen PDE1.
Antigen PDE1 was purified from pigeon dropping extracts by chromatography on DEAE-cellulose and Bio-Gel A-1.5. It was characterized as a glycoprotein, containing 88% protein and 12% carbohydrate, with a molecular weight of approximately 200,000. PDE1 appears to be derived from pigeon intestinal IgA, showing the typical immunoglobulin structure of heavy and light chains. This antigen represents about 24% of the dry weight of the unfractionated pigeon dropping extract.
D000542:Alveolitis, Extrinsic Allergic / Q000276:immunology*; D000818:Animals; D000941:Antigens / Q000032:analysis / Q000302:isolation & purification*; D001716:Bird Fancier's Lung / Q000276:immunology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D010856:Columbidae / Q000276:immunology*; D006801:Humans; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A; D008970:Molecular Weight; D011817:Rabbits
D000941:Antigens; D007070:Immunoglobulin A