Simplified quantitative determination of total fecal bile acids.
To determine total fecal bile acids, these are extracted with diethyl ether after boiling with a solution of potassium hydroxide in ethanediol. After evaporating the ether and dissolving the residue in methanol, the bile acids are directly determined with 3 alpha-hydroxysteroid dehydrogenase. Values for 9 normals are given.
D001647:Bile Acids and Salts / Q000032:analysis*; D005243:Feces / Q000032:analysis*; D005260:Female; D005767:Gastrointestinal Diseases / Q000378:metabolism; D006801:Humans; D008297:Male; D008722:Methods; D013056:Spectrophotometry, Ultraviolet
D001647:Bile Acids and Salts