An in vitro study on the interaction between dimethylnitrosamine and nucleic acids via a microsomal system.
Radioactive alkylated bases, ribose or phosphate, were never found either in acid and alkaline hydrolysates of polyribonucleotides or in alkaline hydrolysates of DNA after incubation with 14C-dimethylnitrosamine (DMNA) in a microsomal system. Two radioactive compounds, which were co-chromatographed with methylamine and N-methylhydrazine, respectively, on column, paper, and thin-layer, were always detected. They differed from the compound derived from 7-methylguanosine after the alkali-mediated fission of the imidazole ring in its molecule. The in vitro system employed well represents the in vivo situation (7-methylguanine which is liberated from DNA after acid hydrolysis); however, it has given results which do not agree with the generally-accepted mechanism of DMNA alkylation at the N-7 position of guanine.
D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D004247:DNA / Q000032:analysis*; D004128:Dimethylnitrosamine / Q000494:pharmacology*; D005001:Ethionine / Q000494:pharmacology; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis; D006868:Hydrolysis; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008744:Methylamines / Q000032:analysis; D008862:Microsomes, Liver / Q000032:analysis*; D009002:Monomethylhydrazine / Q000032:analysis; D009602:Nitrosamines / Q000494:pharmacology*; D011083:Polycyclic Compounds / Q000494:pharmacology; D051381:Rats
D008744:Methylamines; D009602:Nitrosamines; D011083:Polycyclic Compounds; D006147:Guanine; D004247:DNA; D002251:Carbon Tetrachloride; D004128:Dimethylnitrosamine; D009002:Monomethylhydrazine; D005001:Ethionine