The molecular size and shape of liver glycogen.
The molecular-weight distribution of liver glycogen has been established from the analysis of sedimentation rates of fractions separated on sucrose density gradients and from the direct measurement of the diffusion coefficients of these fractions by laser-intensity-fluctuation spectroscopy. Hydrodynamic studies indicated that all fractions of glycogen of mol.wt.exceeding 25x10(6) had about 1.1 g of water per g of polysaccharide associated with them. The hydration and hydrodynamic behaviour of all fractions of mol.wt. exceeding 25x10(6) was similar, whereas smaller fractions behaved anomalously, indicating a substantially different overall structure.
D002499:Centrifugation, Density Gradient; D008112:Liver Glycogen; D008970:Molecular Weight; D013057:Spectrum Analysis; D014783:Viscosity; D014867:Water
D008112:Liver Glycogen; D014867:Water