Formation of oxyadenine metabolites in the rabbit after intravenous administration of adenine.
Adenine, 8-oxyadenine, and 2,8-dioxyadenine were analyzed, by Dowex-50 column chromatography, in rabbit urine four hours after IV infusion of the following doses of adenine in mg/kg: 2, 4, 8.75, 17.5, 35, 70, 140 and 210. The average excretion in mole percentage of the dose was: adenine, 11.1; 8-oxyadenine, 6.1; and 2,8-dioxyadenine, 6.2. The percentage of excretion of 8-oxyadenine and of 2,8-dioxyadenine was unrelated to the dose of adenine. At doses of adenine of 35 mg/kg and above more than 80 per cent of the 2,8-dioxyadenine in the urine was in the form of microscopic pale yellow spherical particles. The particulate 2,8-dioxyadenine was found in the kidney in a very small amount at the dose of 70 mg/kg and in much greater quantities at 140 and 210 mg/kg.
D000225:Adenine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000378:metabolism*; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003460:Crystallization; D005260:Female; D007042:Hypoxanthines / Q000378:metabolism; D007275:Injections, Intravenous; D007668:Kidney / Q000473:pathology; D011817:Rabbits; D012995:Solubility; D014969:Xanthine Oxidase / Q000378:metabolism
D007042:Hypoxanthines; D014969:Xanthine Oxidase; D000225:Adenine