The action of cysteine of PHA-stimulation of lymphocytes. II. Influence on incorporation of 3H-uridine and 14C-leucine.
The influence of short-term preincubation and continuous cultivation with L-cysteine hydrochloride on incorporation of 14C-leucine nad 3H-uridine in cultures of PHA-stimulated and unstimulated lymphocytes was studied. Preincubation during 1 and 2 hours in presence of cysteine inhibited incorporation of these compounds during the first few days of incubation. After 2-hr preincubation, incorporation was first somewhat depressed and then markedly enhanced. Continual presence of cysteine in the medium markedly inhibited incorporation of 3H-uridine and 14C-leucine from the first to last day of incubation.
D000818:Animals; D002470:Cell Survival; D003545:Cysteine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D005260:Female; D007930:Leucine / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051381:Rats; D014529:Uridine / Q000378:metabolism*
D004247:DNA; D007930:Leucine; D003545:Cysteine; D014529:Uridine