title: [A comparative study of the role of creatine phosphokinase isoenzymes in energy metabolism of skeletal and heart muscle].
abstract: It has been shown that the contents of mitochondria and mitochondrial isoenzyme of creatine phosphokinase are almost identical in skeletal and heart muscles. In mitochondria from both types of muscle creatine phosphokinase is functionally coupled to ATP-ADP translocase. This kind of coupling ensures complete conversion of mitochondrial ATP energy into the energy of creatine phosphate and effective control of oxidative phosphorylation by the creatine phosphokinase reaction. It has also been shown that all isoenzymes of creatine phosphokinase from heart and skeletal muscle have very similar kinetic properties. Significant differences have been found to exist between isoenzyme patterns of these muscles and also in distribution of different isoenzymes in the cells. In skeletal muscle cells creatine phosphokinase is present mainly as cytosolic MM isoenzyme; about 6% of total cellular activity is localised also in mitochondria. Due to high activity of cytosolic isoenzyme the total activity of creatine phosphokinase is about three times higher in skeletal muscle than in cardiac muscle. It has been also shown that phosphoenolpyruvate and glucose-6-phosphate do not have any inhibitory effect on creatine phosphokinase.
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000502:physiology*; D003600:Cytosol / Q000201:enzymology; D004734:Energy Metabolism; D007527:Isoenzymes / Q000502:physiology*; D007700:Kinetics; D008931:Mitochondria, Muscle / Q000201:enzymology; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism; D009132:Muscles / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D009928:Organ Specificity; D010085:Oxidative Phosphorylation; D051381:Rats
chemical_list: D007527:Isoenzymes; D000226:Mitochondrial ADP, ATP Translocases; D003402:Creatine Kinase
vernacular_title: Sravnitel'noe issledovanie roli izofermentov kreatinfosfokinazy i energeticheskom metabolizme skeletnoi myshtsy i miokarda