[Microspectrophotometric characteristics of renal enzymes under conditions of acute vascular insufficiency].
The activity of optic density of preparations of cat kidneys by studying lactic, succinic-, glycoso-6-phosphoric-beta-oxybutyrate, NAD.H2-, NADP.H2-tetrazolium-reductases, alkaline and acid phosphatases, was investigated in intact animals, those under hexenal anesthesia, under conditions of a 5-hour hypotension caused by administration of a ganglioblocker (arphonade), 24 hours after the termination of artificial hypotension and in profuse blood letting. Analysis of the optic density distribution curves showed that under conditions of acute vascular insufficiency all the nephron parts reacted by different changes of the enzymatic activity. These changes clearly demonstrated a sharp fall of the energy importance of the main oxidation way (Krebs cycle) and of the prevalence of glycolytic way and pentose shunt under conditions of circulatory hypoxia.
D000135:Acid Phosphatase / Q000378:metabolism; D000208:Acute Disease; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002415:Cats; D006470:Hemorrhage / Q000201:enzymology*; D006651:Histocytochemistry; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D007022:Hypotension / Q000201:enzymology*; D007668:Kidney / Q000201:enzymology*; D008297:Male; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D006884:Hydroxybutyrate Dehydrogenase; D013385:Succinate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase
Mikrospektrofotometricheskaia kharakteristika fermentov pochek v usloviiakh ostroĭ sosudistoĭ nedostatochnosti.