[Proliferation kinetic models for the understanding of the epidermal cell cycle in psoriasis].
Pulse labeling of spontaneous, stimulated or disturbed epidermal cell reinforcements with tritium thymidine offers a simple system to appraise relations of different phases in cell cycle. For example, if the duplication of DNA during the S-phase takes much time, many cells actually synthesizing DNA must be found labeled. Therefore, a high labeling index does not necessarily indicate fast cell cycles (and fast cell cycles do not necessarily produce high labeling indices). The slower S-phases proceed, the less use is for tritium thymidine, offered during the short availability time of a single pulse label. As a result, only few silver grains per cell nucleus are produced in the film emulsion by autoradiography. The typical pattern of this DNA-synthesis would be a high labeling index, combined with a low silver grain index. All other possible combinations and degrees of high, low, normal silver grain indices with high, low or normal labeling indices allow to appraise not only the S-phase duration in comparison with controls, but also in dependence of the other phases, mainly G1, and their duration in cell cycle.
D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D000818:Animals; D000873:Anthracenes / Q000627:therapeutic use; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use; D001345:Autoradiography; D004247:DNA / Q000096:biosynthesis*; D006168:Guinea Pigs; D007700:Kinetics; D011565:Psoriasis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D012867:Skin / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D013936:Thymidine; D014316:Tritium; D014466:Ultraviolet Rays
D000305:Adrenal Cortex Hormones; D000873:Anthracenes; D000891:Anti-Infective Agents, Local; D014316:Tritium; D004247:DNA; D013936:Thymidine
Proliferationskinetische Modelle zum Verständnis des epidermalen Zellzyklus bei Psoriasis.