title: Influenza viruses and staphylococci in vitro: some interactions with polymorphonuclear leucocytes and epithelial cells.
abstract: Bacterial infection contributes substantially to the morbidity and mortality of human influenza. In vitro experiments were performed to test two hypotheses regarding a possible relationship between the virus and bacterial infection. Firstly, maintenance media from tissue and organ cultures infected with influenza virus were tested for the presence of staphylococcal growth-promoting factors; no evidence for these was found. Secondly, we looked for a virus effect on polymorphonuclear leucocyte function. We found that human leucocytes purified from venous blood and exposed to influenza virus responded normally to stimulation of hexose monophosphate shunt activity and chemiluminescence. However, their responses in tests of phagocytic function and of chemotaxis were inhibited. By various criteria this effect was specific to the virus and could be obtained even when only a few virus particles were present per leucocyte. We propose that this is a mechanism by which influenza virus could enhance susceptibility to bacterial infection in the lung.
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D006600:Hexosephosphates / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000378:metabolism*; D009975:Orthomyxoviridae / Q000378:metabolism; D010587:Phagocytosis; D013211:Staphylococcus aureus / Q000378:metabolism
chemical_list: D006600:Hexosephosphates