[Formation of sub-units of antihaemophilic globulin (factor VIII) by means of high centrifugal force (author's transl)].
The human coagulation factor VIII with a molecular weight greater than 1 X 10(6) Daltons forms active subunits under conditions of high centrifugal force, namely, 200.000 g at 20 degrees C for 32 minutes, as demonstrated by sedimentation analysis. This result was confirmed by Sepharose 4 B gel filtration analysis of the ultracentrifuged product, which revealed, in additon to mateial with a molecular weight of more than 1 X 10(6) Daltons, three F VIII-active fractions with smaller molecular weight values. These observations indicate that these sub-units so produced are not united in the starting matrial by covalent bonds.
D055598:Chemical Phenomena; D002621:Chemistry; D005169:Factor VIII / Q000302:isolation & purification*; D006801:Humans; D008970:Molecular Weight; D014461:Ultracentrifugation
D005169:Factor VIII
Bildung von Untereinheiten des antihämophilen Globulins bei der Einwirkung grosser Zentrifugalkräfte