The role of phospholipids in the activity of delta4-3-ketosteroid 5alpha-oxidoreductase of the rat ventral prostate.
The involvement of phospholipids in the activity of 5alpha-reductase from nuclear and microsomal fractions of rat prostate was studied using phospholipases A and C. Phospholipase A reduced the activity of 5alpha-reductase in both subcellular fractions; this effect was not attributable to the hydrolysis products. Phospholipase C stopped only the activity of the nuclear fraction. There was no quantitative correlation between hydrolysis of phospholipids and loss of enzyme activity. The inactivation observed seems to result from phospholipase-induced instability of the enzyme. The results suggest that the altered structure of phospholipids and/or the membranes where 5alpha-reductase is located is responsible for the decrease in activity.
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism*; D010740:Phospholipases / Q000494:pharmacology*; D010743:Phospholipids / Q000494:pharmacology*; D011467:Prostate / Q000201:enzymology*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D010743:Phospholipids; D012710:Serum Albumin, Bovine; D010088:Oxidoreductases; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; D010740:Phospholipases