High-performance liquid chromatographic assay of rat-brain dopamine and norepinephrine.
A high-performance liquid chromatographic method for the assay of dopamine (DM) and norepinephrine (NE) in rat brain has been developed. 3,4-Dihydroxybenzylamine was added to the sample as an internal standard. The isolated amines and the internal standard were subjected to reaction with fluorescamine, separated liquid chromatographically on a glycol-type gel and determined fluorimetrically. The standard deviation of the method was +/-2.7% for DM and +/-5.3% for NE. The contents of DM and NE in rat brain, with or without administration of a tyrosine hydroxylase inhibitor, as measured by the proposed method were always lower than those obtained by the trihydroxyindole method.
D000818:Animals; D001596:Benzylamines / Q000032:analysis; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000032:analysis*; D005450:Fluorescamine; D008722:Methods; D009638:Norepinephrine / Q000032:analysis*; D051381:Rats
D001596:Benzylamines; D005450:Fluorescamine; D004298:Dopamine; D009638:Norepinephrine