An immobilized hexokinase enzyme stirrer for a simple and economical assay of plasma glucose.
We have adapted the hexokinase glucose procedure to an immobilized enzyme stirrer for the determination of glucose concentrations in human blood plasma. The procedure is a fluorometric rate method measuring the formation of NADPH catalyzed by immobilized glucose-6-phosphate dehydrogenase and hexokinase held within a tiny stirrer. The enzyme stirrer is stable for at least two months and can be used over eight-hundred assays without any loss of activity.
D001786:Blood Glucose / Q000032:analysis*; D004800:Enzymes, Immobilized; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism*; D006801:Humans; D008722:Methods; D013050:Spectrometry, Fluorescence
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase