[Determination of the IgA 7 S/IgA 11 S ratio in human bronchial secretion by thin layer gel filtration immunoelectrophoresis (author's transl)].
After extraction by treatment with Ecteola-cellulose, IgA 11 S and IgA 7 S from human bronchial secretion were first separated by thin-layer gel filtration in Ultrogel AcA 3-4, and then were analyzed by crossed immunoelectrophoresis with an immune serum against alpha chain. Precipitation areas were measured separately. A correlation factor was necessary to express IgA 7 S in terms of IgA 11 S: it was calculated with standard curves made with serum and colostral IgA.
D001980:Bronchi / Q000378:metabolism*; D002850:Chromatography, Gel; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007070:Immunoglobulin A / Q000032:analysis*; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000302:isolation & purification; D008722:Methods; D009093:Mucus / Q000032:analysis
D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory
Determination du rapport IgA 7 S/IgA 11 S dans la secretion bronchique humaine par une technique associant gel-filtration en couche mince a une immunoelectrophorese bidimensionnelle.