Expression of alpha-D-mannosidase in man-hamster somatic cell hybrids.
Two types of alpha-D-mannosidase isozymes are present in human white blood cells, human diploid fibroblasts, and HeLa cells. One of these (the S isozyme) constitutes the major alpha-D-mannosidase of the human cells, has a pH optimum of 4.4, and is associated with lysosomes. The other (the F isozyme) is most active at pH 6, is acid labile, and is located in the soluble portion of the cytoplasm. The expression of human lysosomal alpha-D-mannosidase was examined in man-hamster hybrid clones, and was found to be concordant with that of phosphohexose isomerase in 54 of 55 primary clones. A locus specifying human lysosomal alpha-D-mannosidase has therfore been assigned to chromosome 19.
D000818:Animals; D002874:Chromosome Mapping; D002903:Chromosomes, Human, 19-20; D006224:Cricetinae; D003593:Cytoplasm / Q000201:enzymology; D004186:Disaccharidases / Q000096:biosynthesis*; D005347:Fibroblasts / Q000201:enzymology; D005956:Glucose-6-Phosphate Isomerase / Q000096:biosynthesis; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology*; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000096:biosynthesis*; D008247:Lysosomes / Q000201:enzymology; D008361:Mannosidases / Q000096:biosynthesis*; D009439:Neuraminidase / Q000494:pharmacology
D007527:Isoenzymes; D004186:Disaccharidases; D008361:Mannosidases; D009439:Neuraminidase; D005956:Glucose-6-Phosphate Isomerase