Photodynamic action of chlorpromazine on adenovirus 5:repairable damage and single strand breaks.
Chlorpromazine, a substituted phenothiazine, commonly used as a sedative, has been found to photosensitize the inactivation of human adenovirus 5 to wavelengths of light between 330 and 390 nm. The slope of the inactivation curve is three fold greater when fibroblasts from people having xeroderma pigmentosum (XP) were used as viral hosts than when normal fibroblasts were used, showing that at least two-thirds of the damage produced in the virions is repairable by normal human fibroblasts. The phototreatment of chlorpromazine sensitized virions also results in the production of DNA strand breaks, which correlate fairly well with the production of lethal viral damage as measured in XP fibroblasts. These findings suggest that the photosensitization of the skin observed in patients treated with chlorpromazine might be due to DNA damage.
D000260:Adenoviruses, Human / Q000187:drug effects* / Q000528:radiation effects; D002499:Centrifugation, Density Gradient; D002746:Chlorpromazine / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004279:DNA, Viral / Q000528:radiation effects*; D005347:Fibroblasts / Q000382:microbiology; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay; D014776:Virus Cultivation; D014983:Xeroderma Pigmentosum / Q000382:microbiology
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D002746:Chlorpromazine