High-pressure liquid chromatography of glycosphingolipids (with special reference to gangliosides).
The analysis of mixtures of gangliosides from adult human or bovine brain, supplemented with Tay-Sachs ganglioside, and haematoside from dog erythrocytes by high-pressure liquid chromatography using a moving-wire detector system is described. The complete separation of six gangliosides within 40 min has been achieved, using silica as the stationary phase and acidified chloroform-methanol-water mixtures as the eluent on a 25-cm column. Neutral glycosphingolipids, viz., the major components from normal human erythrocytes, can be completely separated on the same column, using non-aqueous and non-acidic eluents. It is shown that the methods described are useful for both analytical and (micro)-preparative purposes.
D000328:Adult; D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D004912:Erythrocytes / Q000032:analysis; D005679:G(M3) Ganglioside / Q000097:blood; D005732:Gangliosides / Q000032:analysis*; D006028:Glycosphingolipids / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008064:Lipidoses / Q000378:metabolism
D005679:G(M3) Ganglioside; D005732:Gangliosides; D006028:Glycosphingolipids