title: The combined action of insulin and phlorizin on transport and metabolism of sugars and nucleotide turnover in the isolated rat diaphragm.
abstract: The combined effects of insulin and phlorizin have been analyzed on two parameters of insulin stimulation in the surviving rat diaphragm : transport and metabolism of sugars, turnover of the phosphate groups of mononucleotides. Phlorizin (c mM) inhibits glucose transport both in the presence and absence of insulin and displays a small additional inhibitory effect on glycogen biosynthesis; with the non metabolizable glucose anlogue 3-O-methyl-D-glucose transport inhibition is demonstrated solely in the presence of insulin. No correlation is demonstrated between the rates of sugar transport, which are strongly phlorizin-sensitive, and the rates of nucleotide turnover, which show no such sensitivity.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D003964:Diaphragm / Q000187:drug effects / Q000378:metabolism*; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000096:biosynthesis; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D010695:Phlorhizin / Q000494:pharmacology*; D010710:Phosphates / Q000378:metabolism; D051381:Rats; D012265:Ribonucleotides / Q000378:metabolism*
chemical_list: D007328:Insulin; D008757:Methylglucosides; D008759:Methylglycosides; D010710:Phosphates; D012265:Ribonucleotides; D000255:Adenosine Triphosphate; D006003:Glycogen; D010695:Phlorhizin; D005947:Glucose