Applications of gas-liquid chromatography in protein chemistry. II. Determination of amide residues in nanomolar amounts of proteins.
A method for the quantitative determination of amide residues in nanomolar amounts of proteins is described, based on dilute acid hydrolysis at 100 degrees, followed by isothermal gas-liquid chromatography of the ammonia released by on-column neutralisation of the hydrolysate and quantitation by means of a conductometric detector. Amide contents are given for twenty well characterised proteins, as well as for asparagine and glutamine.
D000577:Amides / Q000032:analysis*; D000641:Ammonia / Q000032:analysis; D001216:Asparagine / Q000032:analysis; D002849:Chromatography, Gas / Q000295:instrumentation; D002853:Chromatography, Liquid; D005973:Glutamine / Q000032:analysis; D006868:Hydrolysis; D008722:Methods; D008832:Microchemistry; D011506:Proteins / Q000032:analysis*
D000577:Amides; D011506:Proteins; D005973:Glutamine; D001216:Asparagine; D000641:Ammonia