[Methylation of tRNA in Carcinus maenas gonadal cultures: inhibition by purified androgenic gland extract].
Subcultures of ovaries and testis of the crab Carcinus maenas have been performed in the presence of L-[Me-14C]methionine. Introduction in the medium of a chromatographically-purified liposoluble fraction from the androgenic glands of the same animal inhibits the biological methylation of the tRNA of the ovaries by 62%. The inhibition of methylation of five individual bases varies from 45% to 84%. No inhibition of tRNA methylation is observed under the same conditions with testis subcultures.
D000818:Animals; D003386:Brachyura / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D006066:Gonads / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D012343:RNA, Transfer / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology
D014020:Tissue Extracts; D012343:RNA, Transfer; D008715:Methionine
Méthylation du tRNA dans des cultures de gonades de Carcinus maenas: inhibition par un extrait purifié des glandes androgènes.