Psoralen-DNA photoreaction: controlled production of mono- and diadducts with nanosecond ultraviolet laser pulses.
Samples of DNA which contain adducts of a new psoralen derivative, but no cross-links, have been prepared by irradiating mixtures of DNA and the derivative with single, 15-nanosecond pulses of laser light. Succeeding pulses introduce cross-links. The ability to rapidly and selectively create monoadducts and corss-links may allow the use of psoralens as probes of dynamic processes in living cells.
D003090:Coliphages; D003374:Coumarins / Q000031:analogs & derivatives; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D005363:Ficusin; D011564:Furocoumarins; D007700:Kinetics; D007834:Lasers; D010777:Photochemistry; D014466:Ultraviolet Rays
D003374:Coumarins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D011564:Furocoumarins; D005363:Ficusin