title: [Effect of amniotic fluid on the "spontaneous" transformation of embryonic cells of rats].
abstract: While applying amniotic fluid of cow embryos, as one of the components of nutrition medium, the tumor-producing ability of cells is manifested in later period of cultivation. Less malignancy of the transformed cultures was noted, that is supported by a more slow tumor development, by nearly complete absence of metastases, and longer survival of tumor-bearing animals. On the other hand, amniotic fluid renders a somewhat accelerating (2--3 times greater than in the control) effect on the impairment of cultures diploidy. Heteroploidization of the cultures proceeds largely on account of chromosome number shifts from diploid to near-tetraploid amount. Thus, amniotic liquor inhibits the process of "spontaneous" malignant transformation of embryonal cells in prolonged cultivation, simultaneously accelerating the impairment of culture diploidy.
mesh_terms: D000653:Amniotic Fluid; D000818:Animals; D002417:Cattle; D002471:Cell Transformation, Neoplastic; D003470:Culture Media; D046508:Culture Techniques; D004622:Embryo, Mammalian; D005260:Female; D005354:Fibrosarcoma / Q000473:pathology; D009374:Neoplasms, Experimental / Q000473:pathology; D011247:Pregnancy; D051381:Rats
chemical_list: D003470:Culture Media
vernacular_title: Vliianie amnioticheskoĭ zhidkosti na "spontannuiu" transformatsiiu embrional'nykh kletok krys.