Prolactin-induced stimulation of rat renal adenylate cyclase and autoradiographic localization to the distal nephron.
Prolactin was shown to activate adenylate cyclase in broken cellular enzyme preparations from rat renal medulla. Likewise, vasopresin was effective on this enzyme system. Parathyroid hormone was similarly active in the renal cortex. The simultaneous administration of vasopressin and prolactin to medullary kidney slices did not result in an additive effect in stimulating medullary adenyl cyclase. Audioradiographic techniques revealed a selective and prolonged localization of intravenously injected 125I-prolactin to the thick limb of the loop of Henle, the distal tubule and the collecting duct. It is concluded that prolactin activates medullary adenylate cyclase, and may do so by occupying ADH receptors.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D005459:Fluorides / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D007679:Kidney Medulla / Q000187:drug effects / Q000201:enzymology; D007684:Kidney Tubules / Q000201:enzymology*; D007686:Kidney Tubules, Distal / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D010281:Parathyroid Hormone / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D014667:Vasopressins / Q000494:pharmacology
D010281:Parathyroid Hormone; D014667:Vasopressins; D011388:Prolactin; D000262:Adenylyl Cyclases; D005459:Fluorides