Quantitation of cytoplasmic tubulin by radioimmunoassay.
A radioimmunoassay has been developed for the quantitation of crytoplasmic tubulin. It measures tubulin between 20 and 1500 nanograms and does so independently of decay in colchicine-binding activity. In addition, the state of tubulin as subunit or polymer does not alter the measurement.
D000818:Animals; D001665:Binding Sites; D001923:Brain Chemistry; D002645:Chickens; D003078:Colchicine / Q000378:metabolism; D003429:Cross Reactions; D003593:Cytoplasm / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D051379:Mice; D008870:Microtubules / Q000032:analysis; D011863:Radioimmunoassay; D012756:Sheep; D013997:Time Factors; D014404:Tubulin / Q000032:analysis* / Q000378:metabolism
D006023:Glycoproteins; D014404:Tubulin; D003078:Colchicine