[Determination of free cortisol in urine by high-pressure liquid chromatography (author's transl)].
The possibilities were examined for the high-pressure liquid chromatographic analysis of cortisol with methods of adsorption, distribution and reversed-phase chromatography. Free cortisol in urine can be determined by extraction with choloroform and subsequent adsorption chromatography on silica gel with a mobile phase consisting of 1.5% methanol and 0.2% water in chloroform. The time needed for this chromatographic analysis is 10--15 min; the limit of determination is 3 ng of cortisol for one injection.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006854:Hydrocortisone / Q000652:urine*; D008832:Microchemistry
D006854:Hydrocortisone
Hochdruck-flüssigkeitschromatographische Bestimmungsmethode für Cortisol im Urin