[Protein crystals and tubuli bundles in yeast cells. VI. Light-and electron microscopy studies as well as biochemical studies on alcohol dehydrogenase activity of isolated crystals].
Isolated crystals from Saccharomyces carlsbergensis, stabilized by Cd2+ or Zn2+, retain their enzymatic activity as shown by topochemical reactions. During these reactions the crystals disintegrate characteristically. Stages of this disintegration and deposition of reaction products have been demonstrated by light and electron microscopy. Ground plasmatic and mitochondrial alcoholdhydrogenase has been solubilized by means of 0.01 M EDTA from Zn2+ stabilized crystals separated by gel electrophoresis and proved to be active.
D000429:Alcohol Oxidoreductases / Q000032:analysis; D003460:Crystallization; D005656:Fungal Proteins / Q000032:analysis; D012440:Saccharomyces / Q000032:analysis* / Q000201:enzymology / Q000302:isolation & purification / Q000648:ultrastructure
D005656:Fungal Proteins; D000429:Alcohol Oxidoreductases
Proteinkristalle und Tubuli-Bündel in Hefezellen. VI. Licht- und elektronenmikroskopische sowie biochemische Untersuchungen zur Alkoholdehydrogenase-Aktivität isolierter Kristalle.