Studies of protein synthesis during sea urchin oogenesis. II. Synthesis of tubulin.
Sea urchin oocytes were incubated in vitro with [14C]lysine. Tubulin synthesis was demonstrated by gel filtration and/or electrophoresis of the colchicine-tubulin complex precipitated by vinblastine. The incorporation of [14C]lysine into tubulin represented about 12% of the incorporation into the total proteins at vitellogenesis.
D000818:Animals; D011232:Chemical Precipitation; D003078:Colchicine / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D008239:Lysine / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis; D010063:Ovum / Q000378:metabolism*; D012617:Sea Urchins / Q000502:physiology*; D014404:Tubulin / Q000096:biosynthesis*; D014747:Vinblastine
D006023:Glycoproteins; D014404:Tubulin; D014747:Vinblastine; D008239:Lysine; D003078:Colchicine