A simple radiochemical assay of thromboxane B2, 12-hydroxyeicosatetraenoic acid (HETE) and 12-hydroxyheptadecatrienoic acid (HHT) synthetized by human platelets.
A simple radiochemical assay of thromboxane B2, 12-hydroxyeicosatetraenoic acid (HETE) and 12-hydroxyheptadecatrienoic acid (HHT) produced by platelets from arachidonic acid has been described. Moreover, this assay is suitable for measuring thromboxane A2 trapped as 11-mono-O-methylthromboxane B2 and minor products such as prostaglandins E2, F2alpha and D2. The advantage of this technique is discussed as to its ease and specificity.
D001095:Arachidonic Acids / Q000096:biosynthesis / Q000097:blood*; D001792:Blood Platelets / Q000032:analysis* / Q000378:metabolism; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis / Q000097:blood*; D006801:Humans; D006880:Hydroxy Acids / Q000096:biosynthesis / Q000097:blood*; D066298:In Vitro Techniques; D008055:Lipids / Q000097:blood; D008722:Methods; D011714:Pyrans
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D005231:Fatty Acids, Unsaturated; D006880:Hydroxy Acids; D008055:Lipids; D011714:Pyrans; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid