The nature of DNA synthesized in nuclei from mouse L1210 cells.
A DNA-synthesizing system in vitro from mouse L1210 cell nuclei is described. A high proportion of the DNA synthesized by the L1210 nuclei in vitro appeared to be covalently linked complementary DNA. DNA synthesis by L1210 nuclei was not inhibited by the anti-leukaemia compound 4'-9-acridinylamino)-methansulphon-m-anisidine, although this drug binds to isolated DNA and is active against L1210 leukaemia.
D000166:Acridines / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D007700:Kinetics; D007939:Leukemia L1210 / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008970:Molecular Weight; D013424:Sulfanilamides / Q000494:pharmacology
D000166:Acridines; D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D013424:Sulfanilamides