Measurement of enzyme activity and substrates in intestinal mucosa. Evaluation of a system for quality control in clinical and experimental gastroenterology.
Widely used methods in diagnostical and experimental gastroenterology like measuring the protein-and DNA-content and the activity of alkaline phosphatase and sucrase of intestinal mucosa were adapted to a microliter system and partly automatized. With "artificial" control material a system for statistical quality control was established. Lastly the results on up to three years experience with this control system were presented showing an imprecision within run below 5% and an in-imprecision between run below 8% in all methods.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D004247:DNA / Q000378:metabolism; D005399:Fishes; D005753:Gastric Mucosa / Q000201:enzymology* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D013393:Sucrase / Q000378:metabolism
D004247:DNA; D000469:Alkaline Phosphatase; D013393:Sucrase; D005947:Glucose