Gas chromatographic and mass fragmentographic assays of carcinogenic polycyclic hydrocarbon epoxide hydratase activity.
A specific and very sensitive procedure for the determination of epoxide hydratase activity in hepatic microsomes is described. Any polycyclic hydrocarbon epoxide can be used as a substrate; in this study, benzo(a)anthracene-5,6-oxide, benzo(a)pyrene-4,5-oxide and 3-methylcholanthrene-11,12-oxide were utilized. The corresponding trans-diols formed during incubation are separated and evaluated using either an electron-capture gas chromatographic method for the determination of their chloromethyldimethylsilylated derivatives or gas chromatographic-mass fragmentographic measurement of their trimethylsilylated derivatives. Concentrations as low as 1 ng per millilitre of incubation mixture can be estimated.
D000818:Animals; D001551:Benz(a)Anthracenes; D001580:Benzopyrenes; D002273:Carcinogens; D002849:Chromatography, Gas / Q000379:methods; D004851:Epoxide Hydrolases / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006836:Hydro-Lyases / Q000032:analysis*; D007700:Kinetics; D008297:Male; D008748:Methylcholanthrene; D008862:Microsomes, Liver / Q000201:enzymology; D010087:Oxides; D011083:Polycyclic Compounds; D051381:Rats
D001551:Benz(a)Anthracenes; D001580:Benzopyrenes; D002273:Carcinogens; D010087:Oxides; D011083:Polycyclic Compounds; D008748:Methylcholanthrene; D004851:Epoxide Hydrolases; D006836:Hydro-Lyases