[Methylation of liver tissue amino acyl-tRNA-synthetases under different experimental conditions].
Incorporation of 14CH3-methionine into the total preparations of aminoacyl-tRNA-synthetases (ARSases) of the rat liver was studied under conditions of X-irradiation as well as with administration of neostigmine methylsulphate and nicotinic acid to the normal and irradiated animals. 3 h after irradiation of animals with a dose of 800 R specific radioactivity of the enzymic preparation increases on the average by 70%, and irradiation against a background of neostigmine methylsulphate administration causes its decrease on the average by 65% as compared to the norm. Thin-layer chromatography on silicagel showed the presence of such methyllated amino acids as lysine, histidine and arginine in acidic hydrolyzate of ARSase total preparations under all the studied experimental conditions.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism / Q000528:radiation effects*; D000818:Animals; D008099:Liver / Q000201:enzymology / Q000528:radiation effects*; D009388:Neostigmine / Q000494:pharmacology*; D009539:Nicotinic Acids / Q000494:pharmacology*; D051381:Rats
D009539:Nicotinic Acids; D009388:Neostigmine; D000604:Amino Acyl-tRNA Synthetases
Vyvchennia metyliuvannia aminoatsyl-tRNK-syntetaz tkanyny pechinky pry riznykh eksperymental'nykh stanakh