title: A lipid linked oligosaccharide which contains hexosamine, mannose and glucose.
abstract: Previous work showed that liver microsomes catalyze the transfer of glucose from dolichol monophosphate glucose to an endogenous acceptor believed to be a dolichol pyrophosphate derivative of an oligosaccharide. This oligosaccharide has now been prepared on a larger scale so as to permit the determination of its sugars. The purification procedure includes, as a last step, a thin layer chromatography on kieselguhr-silica gel to obviate glucose-containing contaminants. After complete hydrolysis mannose, glucose and a small amount of hexosamine were detected.
mesh_terms: D000818:Animals; D005947:Glucose / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D008070:Lipopolysaccharides / Q000302:isolation & purification; D008099:Liver; D008358:Mannose / Q000032:analysis; D009844:Oligosaccharides / Q000302:isolation & purification; D010743:Phospholipids / Q000032:analysis; D013552:Swine
chemical_list: D006595:Hexosamines; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D010743:Phospholipids; D005947:Glucose; D008358:Mannose