title: Effect of ethidium bromide on the oxidative metabolism and enzyme profiles of Crithidia fasciculata.
abstract: Changes in the metabolism of Crithidia fasciculata ATCC 11745 when grown in the presence of ethidium bromide were studied. Ethidium bromide-grown cells had decreased respiratory activity as measured by oxygen consumption. More than 50% of the organisms cultivated in a defined medium containing 1.0 mg/liter of ethidium bromide became dyskinetoplastic and had decreased activities of particulate succinate and NADH-linked dehydrogenases as well as of soluble isocitrate dehydrogenase. These cells also had increased activities of particulate alpha-glycerophosphate dehydrogenase, soluble alpha-glycerophosphate dehydrogenase, malic enzyme, hexokinase, and malate dehydrogenase. Ethidium bromide-grown cells had a lower level of ATP and contained less DNA than cells grown in its absence.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D004996:Ethidium / Q000494:pharmacology*; D056890:Eukaryota / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects
chemical_list: D000255:Adenosine Triphosphate; D004247:DNA; D004996:Ethidium