title: The effects of actinomycin D and puromycin on the induction of the cytosol enzymes involved in the androgen metabolism by testosterone in female rat liver.
abstract: Induction of the cytosol enzymes involved in the androgen metabolism in the female rat liver by testosterone was studied. The treatment by testosterone propionate increased the production of 5beta-androstane-3alpha,17beta-diol in the 105.000xg supernatant when 4-14C-testosterone was used as a substrate. The conversion of 4-14C-androstenedione to labelled testosterone was increased by the injection of testosterone propionate into female rats. Actinomycin D or puromycin which was administered for four days with daily subcutaneous injections of testosterone propionate prevented the increase of these enzymes by testosterone propionate. Actinomycin D given once with the final injection of testosterone propionate blocked the induction of cytosol 17beta-hydroxysteroid dehydrogenase by the treatment of testosterone propionate, whereas puromycin given in the same manner as actinomycin D had no such a blocking effect on the enzyme. Both of these antibiotics given once with the final injection of testosterone propionate showed no preventing effects on the induction of cytosol delta4-5beta-reductase by testosterone propionate.
mesh_terms: D000728:Androgens / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000201:enzymology; D003609:Dactinomycin / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006913:Hydroxysteroid Dehydrogenases / Q000096:biosynthesis; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D010088:Oxidoreductases / Q000096:biosynthesis; D011691:Puromycin / Q000494:pharmacology*; D051381:Rats; D012737:Sex Factors; D013739:Testosterone / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000728:Androgens; D003609:Dactinomycin; D013739:Testosterone; D011691:Puromycin; D010088:Oxidoreductases; D006913:Hydroxysteroid Dehydrogenases