Studies on Tyzzer's disease: isolation and propagation of Bacillus piliformia.
Mice from a colony with subclinical Tyzzer's disease were treated with prednisolone in order to activate a Bacillus piliformis infection. From the livers of these mice the organism was isolated and serially passaged in embryonated eggs. It  resisted heating at 75 degrees C for 20 minutes and was still infective after storage at -80 degree C for 24 days, but not for 52 days or more.  Infectively was maintained for more than a year by storing infectious  liver tissue at -190 degrees C.  B. piliformis was also isolated from the liver of a mouse with naturally-occurring Tyzzer's disease and maintained for more than 300 passages. Mice inoculated with different egg passages developed fatal Tyzzer's disease and B. piliformis was reisolated from their livers.
D000818:Animals; D001407:Bacillus / Q000254:growth & development / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D003470:Culture Media; D004531:Eggs; D005615:Freezing; D008099:Liver / Q000382:microbiology; D051379:Mice / Q000382:microbiology*; D008807:Mice, Inbred BALB C; D011309:Preservation, Biological; D012376:Rodent Diseases / Q000382:microbiology* / Q000635:transmission
D003470:Culture Media