Identification and quantitation of solubilized I blood group substance by wheat germ agglutinin using quantitative immunoelectrophoresis.
Wheat germ agglutinin (WGA) has been shown to react specifically with solubilized I blood group substance, purified from papain treated human erythrocyte membranes. WGA and I react to form an affinity precipitate in immunodiffusion gels, a reaction which can be blocked by the incorporation of N-acetyl glucosamine into the gel. The I material was a strong inhibitor of both anti-I cold hemagglutination and WGA hemagglutination reactions. Utilizing the techniques of crossed immunoelectrophoresis we have clearly established that WGA and anti-I IgM cold antibody are reacting with the same membrane macromolecule (I antigen). WGA was then used in a rocket affinoelectrophoretic assay system to quantitate I substance. The limits of detection in this system was 25 ng.
D000117:Acetylglucosamine / Q000276:immunology; D001789:Blood Group Antigens; D005690:Galactose / Q000276:immunology; D006801:Humans; D007048:I Blood-Group System; D007106:Immune Sera / Q000494:pharmacology; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007075:Immunoglobulin M / Q000302:isolation & purification; D037102:Lectins; D046911:Macromolecular Substances; D037121:Plant Lectins; D012995:Solubility; D014908:Triticum
D001789:Blood Group Antigens; D007048:I Blood-Group System; D007106:Immune Sera; D007075:Immunoglobulin M; D037102:Lectins; D046911:Macromolecular Substances; D037121:Plant Lectins; D000117:Acetylglucosamine; D005690:Galactose