A rapid radioimmunoassay for serum unconjugated estriol with a directly iodinated estriol radioligand.
Radioiodinated estriol not previously derivatized was found to be immunoreractive and is shown to possess Sephadex-adsorptive characteristics similar to those of tritium-labeled estriol and nonlabeled estriol. Because of these characteristics, radioiodinated estriol is shown to be an acceptable radioligand for use in a rapid and reliable column radioimmunoassay for determination of unconjugated estriol in serum. An evaluation of the estriol column RIA assay with this radioligand is presented.
D002850:Chromatography, Gel; D004964:Estriol / Q000097:blood*; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods*; D011869:Radioligand Assay
D007457:Iodine Radioisotopes; D004964:Estriol