Immune complex-induced platelet aggregation and 3H-serotonin release: microtechnique for an assay of virus and mycoplasma antigens or antibodies.
Human platelets are very sensitive indicators of immune complexes. The microplate test presented employs aggregation and/or release reaction of platelets as parameters of an antibody and antigen assay. The measurement of the release reaction is approximately 5 times more sensitive than the examination of aggregation patterns. Antibody titres of patients' sera attained values of 1/780,000 for adenoviruses and Mycoplasma pneumoniae. The determinations of 3H-serotonin release quantitative and therefore allow the equivalence point of a specific antibody/antigen interaction to be shown. This also provides the basis for reliable titre determinations in patients' sera with non-specific, direct platelet reactivity. If such an antiserum is titrated against the corresponding antigen, the release measurements reveal dose-response relations which are characteristic for an effect medicated by immune complexes.
D000256:Adenoviridae / Q000276:immunology; D000907:Antibodies, Bacterial / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D006801:Humans; D008832:Microchemistry / Q000379:methods; D009174:Mycoplasma / Q000276:immunology; D010974:Platelet Aggregation; D012701:Serotonin / Q000378:metabolism*; D014316:Tritium
D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D000956:Antigens, Viral; D014316:Tritium; D012701:Serotonin