C-21- and 6-hydroxylation of progesterone by rabbit liver subcellular fractions.
In vitro C-21-hydroxylation of [3H]progesterone (P) has been demonstrated for the first time with rabbit liver microsomes and mitochondria. Deoxycorticosterone (DOC) was rigorously characterized as a metabolite of both mitochondrial and microsomal metabolism, whereas 6alpha-hydroxy DOC and 6alpha-hydroxy P were only identified as microsomal metabolites. 6beta-Hydroxy metabolites were also detected but were of less quantitative significance. Formation of 6alpha-hydroxy P and 6alpha-hydroxy DOC increased steadily between 5 and 120 min of incubation with the microsomal fraction, whereas DOC increased up to 30 min of incubation and then declined. Maximal yield of DOC was 25.9 and 22.5 pmol/mg protein with the mitochondrial and microsomal fractions, respectively.
D000818:Animals; D002458:Cell Fractionation; D003900:Desoxycorticosterone / Q000378:metabolism; D005260:Female; D006900:Hydroxylation; D008862:Microsomes, Liver / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D011817:Rabbits; D013237:Stereoisomerism; D013250:Steroid Hydroxylases / Q000378:metabolism; D013997:Time Factors
D003900:Desoxycorticosterone; D011374:Progesterone; D013250:Steroid Hydroxylases