[Quantitative determination of hippuric acid in urine using high pressure liquid chromatography (author's transl)].
A simple method to determine hippuric acid in urine by high pressure liquid chromatography is described. An ion exchange column is used for the separation. The detection is carried out with a photometer. The analytical criteria of the reliability of the method are tested. The results for the precision and the accuracy of the method meet the guidelines of the statistical quality control. 34 urinary constituents were tested for their interference in order to examine the specificity. Measuring urine samples from 46 normal persons yielded hippuric acid concentrations between 160 and 2010 mg/1. The median was 400 mg/1, equivalent to 357 mg/g creatinine. These results are compared to those given in the literature. Comparative investigations of those urine samples by a gas chromatographic method matched well, with a coefficient of regression of 0.86.
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000652:urine; D005069:Evaluation Studies as Topic; D006626:Hippurates / Q000652:urine*; D006801:Humans; D008722:Methods
D006626:Hippurates; D003404:Creatinine
Quantitative Bestimmung von Hippursäure im Urin mit der Hochdruck-Flussigkeits-Chromatographie.