title: The interaction of p-nitrophenyl carbamate with urease.
abstract: 1. p-Nitrophenyl carbamate and thiourea have been shown to be substrates for urease (urea amidohydrolase, EC 3.5.1.5) 2. Urease has been shown to have a lower Km, 0.67 mM, with p-nitrophenyl carbamate than with urea, 2.0 mM. 3. The V of urease for the hydrolysis of urea, p-nitrophenyl carbamate and thiourea has been shown to be the same, indicating a common rate-limiting step. 4. A mechanism has been proposed for urease where the initial rate-limiting step is the release of a molecule of ammonia from the substrates.
mesh_terms: D002219:Carbamates / Q000494:pharmacology*; D007700:Kinetics; D010944:Plants / Q000201:enzymology; D013056:Spectrophotometry, Ultraviolet; D014510:Urease / Q000378:metabolism*
chemical_list: D002219:Carbamates; D014510:Urease