Synthesis of polyprenol-monophosphate- beta -galactose by acetobacter xylinum.
A particulate enzyme preparation from Acetobacter xylinum synthesizes ficaprenol-monophosphate-beta-galactose from ficaprenol monophosphate (FMP) and UDP-galactose in the presence of detergent. The product has the same properties as those previously reported for the compound formed with the endogenous acceptor. Dolichol-monophosphate (DolMP) is also a good galactose acceptor but the product obtained has different properties. Lipid extracts from Acetobacter contain galactose acceptor capacity which is lost by mild acid treatment. FMP behaves in a similar manner but DolMP is resistant to this treatment. It is concluded that the endogeneous acceptor is an allylic phosphate ester of a polyprenol.
D041822:Gluconacetobacter xylinus / Q000378:metabolism*; D006600:Hexosephosphates / Q000096:biosynthesis*; D007700:Kinetics; D011092:Polyethylene Glycols / Q000494:pharmacology; D011134:Polysaccharides / Q000096:biosynthesis; D013729:Terpenes / Q000096:biosynthesis*; D014531:Uridine Diphosphate Galactose / Q000378:metabolism
D006600:Hexosephosphates; D011134:Polysaccharides; D013729:Terpenes; D014531:Uridine Diphosphate Galactose; D011092:Polyethylene Glycols