title: Gas-liquid chromatographic determination of 1,3-dihydro-3-phenylspiro(isobenzofuran-1,4-piperidine), HP 505, in biological fluids using a nitrogen-specific detector.
abstract: A gas chromatographic method for the determination of 1,3-dihydro-3-phenylspiro[isobenzo-1,4-piperidine], HP 505, in plasma red blood cells and urine has been developed. HP 505 and internal standard are extracted from basified fluid with hexane and then back extracted into acetic acid. After re-extraction into hexane, HP 505 and internal standard are analysed by gas-liquid chromatography as the N-propionyl derivatives using a nitrogen-specific detector. Concentrations of HP 505 can be measured over the range 2-100 ng/ml plasma. The method has been applied to the analysis of biological fluids from volunteers receiving oral doses of HP 505.
mesh_terms: D001572:Benzofurans / Q000032:analysis* / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods; D002853:Chromatography, Liquid; D006801:Humans; D009584:Nitrogen / Q000032:analysis; D010880:Piperidines / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D001572:Benzofurans; D010880:Piperidines; C014903:HP 505; D009584:Nitrogen