title: Sequence analysis of the nicks and termini of bacteriophage T5 DNA.
abstract: Bacteriophage T5 DNA, when isolated from mature phage particles, contains several nicks in one of the two strands. The 5'-terminal nucleotides at the nicks were labeled with polynucleotide kinase and [gamma-32P]ATP, and the 3'-terminal nucleotides were labeled with Escherichia coli DNA polymerase I and [alpha-32P]dGTP. The sequences around the nicks were analyzed by partial nuclease digestion followed by homochromatography fractionation of the resulting oligonucleotides. The nicks had at least the sequence -PuOH pGpCpGpC- in common. In addition, the two 5' external termini had the first seven nucleotides in common.
mesh_terms: D001483:Base Sequence; D003090:Coliphages / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000032:analysis
chemical_list: D004279:DNA, Viral; D009711:Nucleotides