title: Nonparallel secretion of enzymes in human duodenal juice and pure pancreatic juice collected by endoscopic retrograde catheterization of the papilla.
abstract: Duodenal juice was collected from 50 healthy patients in the basal state, and after intravenous injection of 1 clinical unit of secretin kg-1 + 3 Crick-Harper-Raper units of cholecystokinin-pancreozymin (CCK-PZ) kg-1. Amylase to chymotrypsin and amylase to lipase ratios were significantly decreased by stimulation. Pure pancreatic juice was collected from 7 healthy subjects by endoscopic retrograde catheterization of the papilla. Secretin (1 clinical unit kg-1 hr-1) was infused intravenously during 30 min, and a single intravenous injection of CCK-PZ (3 Crick-Harper-Raper units kg-1) was done at the 15th min of the secretin infusion. Chymotrypsinogen and lipase secretions did not parallel that of amylase during CCK-PZ stimulation. As in the first set of experiments, amylase secretion was proportionally less stimulated by CCK-PZ injection than were chymotrypsinogen and lipase secretions. However, in approximately 20% of subjects the secretion of the enzymes was parallel in both duodenal and pancreatic juice.
mesh_terms: D014670:Ampulla of Vater; D000681:Amylases / Q000378:metabolism; D002766:Cholecystokinin; D002918:Chymotrypsin / Q000378:metabolism; D004386:Duodenum / Q000201:enzymology*; D004724:Endoscopy / Q000379:methods*; D005260:Female; D006801:Humans; D007441:Intubation, Gastrointestinal / Q000379:methods*; D008049:Lipase / Q000378:metabolism; D008297:Male; D008722:Methods; D010179:Pancreas / Q000378:metabolism*; D010189:Pancreatic Juice / Q000201:enzymology*; D013048:Specimen Handling; D013268:Stimulation, Chemical
chemical_list: D002766:Cholecystokinin; D008049:Lipase; D000681:Amylases; D002918:Chymotrypsin