title: Fluorescence decay and quantum yield characteristics of acridine orange and proflavine bound to DNA.
abstract: Fluorescence properties (quantum yield, decay curve, lifetime and polarization) of acridine orange and proflavine bound to DNA were examined as a function of nucleotide to dye (P/D) ratio. First, mean fluorescence lifetimes were determined by the phase-shift measurements. The lifetime and quantum yield of acridine orange increased in a parallel fashion with increasing P/D ratio. There was no parallel relation between the lifetime and quantum yield for proflavine; the lifetime showed a minimum around  P/D equals 10...
mesh_terms: D000166:Acridines / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000378:metabolism*; D004735:Energy Transfer; D005453:Fluorescence; D011066:Poly C / Q000031:analogs & derivatives / Q000378:metabolism; D011068:Poly G / Q000031:analogs & derivatives / Q000378:metabolism; D011067:Poly dA-dT / Q000378:metabolism; D011370:Proflavine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D000166:Acridines; D011068:Poly G; D011067:Poly dA-dT; D011066:Poly C; D004247:DNA; D011370:Proflavine