An analysis of the bovine genome by density gradient centrifugation: fractionation in Cs2SO4/3,6-bis(acetatomercurimethyl)dioxane density gradient.
The fractionation of calf thymus DNA by centrifugation in density gradients of Cs2SO4/BAMD, where BAMD = 3,6-bis(acetatomercurimethyl)dioxane, is described. A large-scale separation of (dG+dC)-rich DNA fractions has been obtained, allowing the relative amounts of minor and satellite components in the bovine genome to be precisely assessed.
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient / Q000379:methods; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000302:isolation & purification*; D004276:DNA, Satellite / Q000302:isolation & purification; D003854:Deoxyribonucleotides / Q000032:analysis; D005838:Genotype; D008767:Methylmercury Compounds; D013950:Thymus Gland / Q000032:analysis*
D004276:DNA, Satellite; D003854:Deoxyribonucleotides; D008767:Methylmercury Compounds; C006759:3,6-bis(acetatomercurimethyl)dioxane; D004247:DNA