Column cation-exchange separation of melanin-related metabolites in  urine from cases of melanoma.
We describe recent developments in the use of a stable free radical, diphenylpicrylhydrazyl, as a colorimetric detector of reducing metabolites in melanoma urines. A procedure for buffering aqueous/solvent reagent solutions is presented, and examples of the baseline stability achieved for reference chromatograms and urine samples are provided. Chromatograms of phaeomelanin precursors and of an extract of a highly pigmented hamster melanoma are also presented. Identities are tentatively assigned for some of the chromatographic peaks that have previously been correlated with disease, including isomers of cysteinyldopa, and observations of new pigment- and tumor-related metabolites in the chromatograms are noted.
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D006224:Cricetinae; D003548:Cysteinyldopa / Q000652:urine; D006801:Humans; D006834:Hydrazines; D008543:Melanins / Q000652:urine*; D008545:Melanoma / Q000652:urine*; D010851:Picrates
D006834:Hydrazines; D008543:Melanins; D010851:Picrates; D003548:Cysteinyldopa