title: Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine.
abstract: A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.
mesh_terms: D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D006868:Hydrolysis; D008403:Mass Screening; D008722:Methods; D008832:Microchemistry; D010080:Oxazoles / Q000652:urine; D010389:Pemoline / Q000652:urine*
chemical_list: D010080:Oxazoles; D010389:Pemoline