Microbial transformation of antibiotics. Clindamycin ribonucleotides.
Addition of clindamycin to whole-cell cultures of Streptomyces coelicolor Müller resulted in the loss of in vitro activity against organisms sensitive to clindamycin. Incubation of such culture filtrates with crude alkaline phosphatase generated a biologically active material identified as clindamycin. Fermentation broths containing inactivated clindamycin yielded clindamycin 3-ribonucleotides and clindamycin 3-phosphate the structure of which was established by physicochemical and enzymatic means. Attempts to transform clindamycin to clindamycin 3-ribonucleotides by lysates or partially purified enzyme preparations from S. coelicolor have failed.
D000818:Animals; D001419:Bacteria / Q000187:drug effects; D001711:Biotransformation; D002474:Cell-Free System / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D002981:Clindamycin / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D005285:Fermentation; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009943:Organophosphorus Compounds / Q000378:metabolism; D010084:Oxidation-Reduction; D012265:Ribonucleotides / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors
D009943:Organophosphorus Compounds; D012265:Ribonucleotides; D002981:Clindamycin