Isolation and characterization of 7-hydroxydiftalone beta-glucuronide.
Metabolic studies on diftalone (I), a new non-steroidal antiinflammatory agent, demonstated that 7-hydroxydiftalone beta-glucuronide (V) is the main urinary metabolite. The isolation of (V) from the urine of a dog treated with 14C-diftalone was performed by liquid-liquid partition after precolation through Amberlite XAD-2 and IRC-50 (H+). All the steps were followed by radio-detection and by thin layer radio-chromatography. Compound (V) was characterized mainly by mass spectrometry after esterification with diazomethane and silylation with BSA.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification* / Q000652:urine; D001711:Biotransformation; D004285:Dogs; D005965:Glucuronates / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D011724:Pyridazines / Q000302:isolation & purification* / Q000652:urine
D000893:Anti-Inflammatory Agents; D005965:Glucuronates; D011724:Pyridazines