Effect of cobalt on the synthesis of liver microsomal cytochromes.
1. The amount of cytochrome b5 was not changed significantly by a single injection of cobalt (60 mg/kg body weight) or by daily injection of cobalt (30 mg/kg body weight) for 4 days or 8 days. On the other hand, the amount of cytochrome P-450 was depressed strongly by both cobalt treatments. 2. The incorporations of [3H]leucine as well as 5-amino[3H]levulinate into cytochrome b5 in cobalt-treated animals were almost the same as those in the controls 5 h after injections of the radioisotopes, whereas the radioactivity of heme labelled with 5-amino[3H]levulinate in microsomal residues after trypsin digestion, which would consist mainly of cytochrome P-450, was higher in the controls than in cobalt-treated animals after 5 h.
D000622:Aminolevulinic Acid / Q000378:metabolism; D000818:Animals; D003035:Cobalt / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D003580:Cytochromes / Q000096:biosynthesis*; D006418:Heme / Q000096:biosynthesis; D007930:Leucine / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D014357:Trypsin
D003580:Cytochromes; D003035:Cobalt; D006418:Heme; D000622:Aminolevulinic Acid; D003577:Cytochrome P-450 Enzyme System; D014357:Trypsin; D007930:Leucine