The submicrosomal distribution in rat and boar testis of some enzymes involved in androgen and 16-androstene biosynthesis.
The submicrosomal localization of enzymes involved in androgen and 16-androstene biosynthesis in rat and boar testis tissue has been studied. 16-androstene production was not evident in the rat testis but two enzymes concerned with androgen generation (17 alpha-hydroxy C21 steroid C-17,20 lyase; 17 beta-hydroxysteroid dehydrogenase, were found predominantly in agranular microsomes. In the boar testis the C-17,10 lyase had a similar distribution to the rat enzyme but the 17 beta-hydroxysteroid dehydrogenase was found to be evenly distributed between the two microsomal types. The enzyme system, "andien-betha synthetase", involved in the conversion of pregnenolone to 5,16-androstadien-3 beta-ol, was found mainly (66%) in the agranular microsomes but had a lower specific activity than those of the two enzymes of androgen biosynthesis that have been studied.
D000728:Androgens / Q000096:biosynthesis*; D000736:Androstenes / Q000096:biosynthesis*; D000818:Animals; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D006914:Hydroxysteroids; D008190:Lyases / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D008861:Microsomes / Q000201:enzymology* / Q000648:ultrastructure; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D013552:Swine; D013737:Testis / Q000201:enzymology*
D000728:Androgens; D000736:Androstenes; D006914:Hydroxysteroids; D010088:Oxidoreductases; D006913:Hydroxysteroid Dehydrogenases; D008190:Lyases