Identification of aldophosphamide as a metabolite of cyclophosphamide in vitro and in vivo in humans.
Aldophosphamide (NSC 254), a putative key metabolite of cyclophosphamide, has now been isolated as a cyanohydrin derivative from an incubation mixture of cyclophosphamide with mouse liver microsomes in vitro and from the plasma of a cyclophosphamide-treated patient. The cyanohydrin has been shown to be identical with an authenic synthetic sample by mass spectrometry and combined gas chromatography-mass spectrometry.
D000447:Aldehydes / Q000302:isolation & purification; D000818:Animals; D002849:Chromatography, Gas; D003520:Cyclophosphamide / Q000097:blood / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D009588:Nitrogen Mustard Compounds / Q000302:isolation & purification*; D009943:Organophosphorus Compounds / Q000302:isolation & purification
D000447:Aldehydes; D009588:Nitrogen Mustard Compounds; D009943:Organophosphorus Compounds; D003520:Cyclophosphamide