Effect of homopolyribonucleotides on messenger ribonucleoprotein particles.
A discrete set of polypeptides copurify with and appear to be specifically attached to mRNA from polysomes of eukaryotic cells. This report describes the effect of homopolyribonucleotides on mRNA-protein complexes separated from ribosome subunits by oligo(dT)-cellulose chromatography. It is shown that poly (U) and poly (A) can release mRNA-protein complexes adsorbed to oligo(dT)-cellulose, whereas poly (C) and poly(I) are much less effective in this process. Analysis of polyribonucleotide released material showed that poly(U) effectively dissociated the mRNA-protein complexes while poly(A) caused no or only partial derangement of these particles. The specificities seen in the polyribonucleotide effects in turn suggest a high degree of specificity in the interaction between the proteins and mRNA.
D000256:Adenoviridae; D000327:Adsorption; D002482:Cellulose / Q000031:analogs & derivatives; D055598:Chemical Phenomena; D002621:Chemistry; D006367:HeLa Cells; D009698:Nucleoproteins / Q000302:isolation & purification; D010455:Peptides / Q000032:analysis; D011061:Poly A / Q000494:pharmacology; D011066:Poly C / Q000494:pharmacology; D011069:Poly I / Q000494:pharmacology; D011072:Poly U / Q000494:pharmacology; D011131:Polyribonucleotides / Q000494:pharmacology*; D011132:Polyribosomes / Q000032:analysis; D012333:RNA, Messenger; D012261:Ribonucleoproteins / Q000302:isolation & purification
D009698:Nucleoproteins; D010455:Peptides; D011131:Polyribonucleotides; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D011061:Poly A; D011069:Poly I; D011072:Poly U; D011066:Poly C; D002482:Cellulose