title: Cryopreservation of aster yellows agent in whole leafhoppers.
abstract: Aster yellows agent was preserved in an infectious state in leafhoppers (Macrosteles fascifrons) that had been fed on infected asters and then frozen and stored as whole insects at -64 degrees C for up to 2 years. No difference in infectivity was observed between extracts prepared from insects stored at -28 and -64 degrees C for up to 21 weeks. Three solutions (glycine + magnesium chloride, glycine + magnesium chloride + sodium sulphate, and phosphate-buffered saline) were found to be equally suitable in preparing extracts from frozen leafhoppers.
mesh_terms: D000818:Animals; D002712:Chlorides; D005615:Freezing; D005998:Glycine; D007313:Insecta / Q000382:microbiology*; D008274:Magnesium; D010710:Phosphates; D010935:Plant Diseases; D010942:Plant Viruses / Q000472:pathogenicity; D011309:Preservation, Biological; D012996:Solutions; D013447:Sulfites; D013696:Temperature
chemical_list: D002712:Chlorides; D010710:Phosphates; D012996:Solutions; D013447:Sulfites; D008274:Magnesium; D005998:Glycine