Subcellular distribution, a factor in risk evaluation of pentachlorophenol.
Pentachlorophenol (PCP) is a potent uncoupler of mitochondrial phosphorylation in vitro and also interferes with microsomal detoxication functions in vitro. This favours flavin mediated oxygenation compared with flavin cytochrome P-450 dependent reactions. Gas chromatographic analysis of subcellular fractions, obtained by zonal centrifugation showed markedly lower PCP concentration in mitochondria and a high accumulation in microsomes compared with cytosol. This increases the likelihood that PCP in vivo causes a malfunction in microsomal detoxication.
D000107:Acetylation; D000818:Animals; D002474:Cell-Free System; D002501:Centrifugation, Zonal; D002733:Chlorophenols / Q000378:metabolism*; D002849:Chromatography, Gas; D003600:Cytosol / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000648:ultrastructure*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008745:Methylation; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D010416:Pentachlorophenol / Q000009:adverse effects / Q000378:metabolism*; D051381:Rats; D012306:Risk; D013347:Subcellular Fractions / Q000378:metabolism*
D002733:Chlorophenols; D010416:Pentachlorophenol