Diacylglycerol acyltransferase from rat liver microsomes. Separation and acyl-donor specificity.
1. Diacylglycerol acyltransferase was resolved from rat liver microsomes and separated from glycerolphosphate acyltransferase and 1-acylglycerolphosphate acyltransferase. The separation was achieved by sucrose-density-gradient centrifugation of the enzyme preparation which was obtained by molecular-sieve chromatography of microsomes solubilized with a nonionic detergent, Triton X-100. 2. Although diacylglycerol acyltransferase and 1-acylglycerolphosphorylcholine acyltransferase were not separated from each other, the two acyltransferases were distinguishable with respect to heat stability and sensitivity to sulfhydryl-binding reagents. 3. Studies with the diacylglycerol acyltransferase preparation obtained have shown that this enzyme possesses a broad acyl-donor specificity, utilizing saturated, monoenoic, dienoic and tetraenoic fatty acyl-CoA thioesters efficiently. 4. A simplified assay method for diacylglycerol acyltransferase is described.
D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000302:isolation & purification; D000123:Acetyltransferases / Q000302:isolation & purification; D000217:Acyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D004075:Diglycerides; D004355:Drug Stability; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000302:isolation & purification; D006358:Hot Temperature; D008862:Microsomes, Liver / Q000201:enzymology*; D011485:Protein Binding; D013329:Structure-Activity Relationship; D013439:Sulfhydryl Reagents / Q000378:metabolism
D004075:Diglycerides; D013439:Sulfhydryl Reagents; D000217:Acyltransferases; D000123:Acetyltransferases; D005992:Glycerol-3-Phosphate O-Acyltransferase; D008243:1-Acylglycerophosphocholine O-Acyltransferase