title: The staphylococcal scalded skin syndrome. An experimental histochemical and electron microscopic study.
abstract: Histochemical and electron microscopic studies were carried out on the newborn mouse model of the staphylococcal scalded skin syndrome to investigate the mechanism of action of the staphylococcal epidermolytic toxin that causes it. Histochemical studies showed that an intra-epidermal split develops below the subcorneal zone which is rich in catabolic enzymes (the so-called esterase-acid phosphatase-rich band). However, histochemical alterations in the enzyme pattern could not be demonstrated. The earliest change revealed by electron microscopy was a widening of the intercellular space, with the formation of microvilli at the level between the stratum spinosum and stratum granulosum where the split later occurs. A clearing of the peripheral cytoplasm along the cell membranes was also revealed. In pre-split areas, adhesion between cell membranes of adjacent cells seems to be lost; desmosomes continue to hold the cells together but the split develops when these are broken by mechanical pressure. Later, damaged cell membranes may be seen. Extracellular keratinosomes remain unchanged. Although these findings do not agree with the already divergent results of other studies, they help support the findings of all groups that cases of the Lyell syndrome produced by staphylococci do not occur through necrolysis; it is therefore inappropriate to continue applying the term 'toxic epidermal necrolysis' to such cases.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001427:Bacterial Toxins; D001681:Biological Assay; D051379:Mice; D008854:Microscopy, Electron; D012867:Skin / Q000648:ultrastructure; D013203:Staphylococcal Infections / Q000150:complications*; D013210:Staphylococcus / Q000302:isolation & purification*; D013262:Stevens-Johnson Syndrome / Q000201:enzymology / Q000209:etiology / Q000382:microbiology / Q000473:pathology*; D014118:Toxins, Biological / Q000032:analysis
chemical_list: D001427:Bacterial Toxins; D014118:Toxins, Biological