Metabolism of estradiol by uterine peroxidase: nature of the water-soluble products.
The nature of the water-soluble products formed by incubating labelled estradiol with uterine peroxidase in the presence of H2O2 and tyrosine was examined by two-dimensional thin-layer chromatography and high voltage electrophoresis. It was shown that the steroid and amino acid were associated in a 1:2 or 1:3 ratio and evidence was provided by 3H-exchange for the interaction of tyrosine with ring A of estradiol at C-2 and C-4. The possible role of estrogen-induced peroxidase in the uterus in vivo is discussed.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D014443:Tyrosine / Q000378:metabolism*; D014599:Uterus / Q000201:enzymology
D000418:Albumins; D014443:Tyrosine; D004958:Estradiol; D006861:Hydrogen Peroxide; D010544:Peroxidases