title: An immobilized hexokinase enzyme stirrer for a simple and economical assay of plasma glucose.
abstract: We have adapted the hexokinase glucose procedure to an immobilized enzyme stirrer for the determination of glucose concentrations in human blood plasma. The procedure is a fluorometric rate method measuring the formation of NADPH catalyzed by immobilized glucose-6-phosphate dehydrogenase and hexokinase held within a tiny stirrer. The enzyme stirrer is stable for at least two months and can be used over eight-hundred assays without any loss of activity.
mesh_terms: D001786:Blood Glucose / Q000032:analysis*; D004800:Enzymes, Immobilized; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism*; D006801:Humans; D008722:Methods; D013050:Spectrometry, Fluorescence
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase