[Release of DNA from the nuclei of hepatocytes of rats following their treatment with heparin].
The influence of heparin on the protein fractions and DNA of the isolated nuclei of hepatocytes was studied by biochemical and cytospectrophotometrical methods on Wistar rats. It was found that 0.05% sodium heparinate solution blocked 95% of the histones, not influencing the non-histone proteins of the cell nucleus. Histone blocking was connected with the DNA release into the incubation medium in the amounts proportional to the incubation period.
D000818:Animals; D004247:DNA / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009698:Nucleoproteins / Q000378:metabolism; D051381:Rats
D006657:Histones; D009698:Nucleoproteins; D006493:Heparin; D004247:DNA
Vykhod DNK iz iader gepatotsitov krys pri obrabotke ikh geparinom.