title: Interaction of the phosphatidylcholine exchange protein with phospholipids. A fluorescence and circular dichroism study.
abstract: The phosphatidylcholine exchange protein from bovine liver has a fluorescence emission maximum at 327 nm. Fluorescence was enhanced in the presence of vesicles containing phosphatidylcholine and various amounts of either phosphatidic acid or phosphatidylglycerol. From the increase in fluorescence it was derived that the apparent dissociation constant of the exchange protein-vesicle complex decreased with an increased vesicle content of acidic phospholipids. Fluorescence indicated that the exchange protein interacted with lysophosphatidylcholine micelles at pH 3.5, 5.9 and 8.5. The increase in fluorescence was most prominent at the acidic pH. Circular dichroism indicated that the alpha-helix content of the native protein was low between pH 3.6 and 8.0. Interaction with lysophosphatidylcholine micelles had a negligible effect on the secondary structure of the protein, except at pH 3.6 where distinct minima at 208 nm and 220 nm in the circular dichroic spectrum became apparent.
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D002942:Circular Dichroism; D004146:Dioxanes; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011487:Protein Conformation; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002352:Carrier Proteins; D004146:Dioxanes; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010743:Phospholipids