Detection of platelet-bound IgG with 125I-labelled staphylococcal protein A.
125I-labelled protein A from Staphylococcus aureus binds effectively with platelets coated either with specific antibodies or with unrelated immune complexes. Protein A can therefore be used to measure these serum factors. A prior dilution of the serum enhances markedly the reactivity of the serum in idopathic thrombocytopenic purpura. This may be due to abolished inhibition exerted by normal monomeric IgG.
D000936:Antigen-Antibody Complex; D001426:Bacterial Proteins; D001666:Binding Sites, Antibody; D001792:Blood Platelets / Q000276:immunology* / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism*; D011485:Protein Binding; D013211:Staphylococcus aureus
D000936:Antigen-Antibody Complex; D001426:Bacterial Proteins; D007074:Immunoglobulin G