title: Sesquiterpene lactones. XIX. Preliminary studies on the mode of action. Inhibition of synthesis of tumor cell protein and RNA.
abstract: The isotopic examinations of the mode of action of cytostatic active acid 8a-isobuetyric-14,15-dihydroxy-germacran-1 (10),4,11(13)-trienolide 12(6) (alatolide) have demonstrated that the compound at concentration 5-6 X 10(-5) M induces defective changes in HeLa cells resulting in the inhibition of protein synthesis in 93-8% and RNA in 91-9% and, in consequence, cell death. At lower concentrations 2-8 X 10(-6) M, the compound inhibits protein synthesis in 73% and RNA in 5-7%. The direct influence of alatolide on DNA synthesis is doubtful.
mesh_terms: D002460:Cell Line; D003864:Depression, Chemical; D005260:Female; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D007783:Lactones / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D012717:Sesquiterpenes / Q000494:pharmacology*; D014158:Transcription, Genetic
chemical_list: D007783:Lactones; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D012717:Sesquiterpenes