Aflatoxicol M1, a new metabolite of aflatoxicol.
1. Using dog liver microsomal preparations, we have isolated and identified a new metabolite of aflatoxicol, aflatoxicol M1; this metabolic transformation is analogous to the conversion of aflatoxin B1 to aflatoxin M1. 2. Aflatoxicol M1 can also be produced from aflatoxin M1 by a reductase present in rabbit liver cytosol, a reaction analogous to the reduction of aflatoxin B1 to aflatoxicol. 3. In addition, aflatoxicol M1 can be oxidized to aflatoxin M1 by a carbon monoxide-insensitive dehydrogenase activity associated with human liver microsomes, a reaction analogous to the production of aflatoxin B1 from aflatoxicol by this fraction. 4. Ultra-violet, fluorescence, and mass spectral characteristics of aflatoxicol M1 are described. 5. The significance of this new aflatoxin metabolite and the possibility that it may be biologically active are discussed.
D000348:Aflatoxins / Q000378:metabolism*; D000818:Animals; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D004285:Dogs; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D013058:Mass Spectrometry; D008647:Mesocricetus; D010088:Oxidoreductases / Q000378:metabolism; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013347:Subcellular Fractions / Q000201:enzymology
D000348:Aflatoxins; D010088:Oxidoreductases