title: Isolation and characterization of further defective clones of a temperature sensitive mutant (ts-1) of respiratory syncytial virus.
abstract: After exposure of the temperature sensitive ts-1 mutant of respiratory syncytial virus to the chemical mutagne, nitrosoguanidine (NG), 2 clones of virus were recovered which were more temperature sensitive and stable genetically than the ts-1 mutant. The initial criterion used for selection of the 2 clones was decreased ability to produce plaques at 36 degrees C. Subsequently it was shown that the 2 clones grew less well at the restrictive temperatures of 37 degrees and 38 degrees C than did the ts-1 parent. Peak titers of the NG derived clones were decreased 10--30 fold at 37 degrees C and over 100-fold at 38 degrees C compared to ts-1. Complementation analysis indicated that the NG mutants retained the same complementation pattern as the ts-1 parent.
mesh_terms: D002460:Cell Line; D005816:Genetic Complementation Test; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines / Q000494:pharmacology; D012136:Respiratory Syncytial Viruses / Q000187:drug effects / Q000254:growth & development*; D013696:Temperature; D014779:Virus Replication
chemical_list: D009153:Mutagens; D009604:Nitrosoguanidines