Two microsomal-associated iron-binding proteins observed in rat small intestinal cells during iron absorption.
Acrylamide gel electrophoresis of microsomal protein obtained from rat small intestinal mucosal cells, after an injection of [3H]leucine, demonstrated increased quantities of two soluble iron-binding proteins during iron absorption, one with a high molecular weight (about 400 000) and the other of intermediate molecular weight (80 000). Both proteins were present in a ribosomal-enriched sub-fraction obtained during purification of the microsomal membrane but were not identified among the purified membrane proteins.
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism*; D005260:Female; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats; D012270:Ribosomes / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
D002352:Carrier Proteins; D007501:Iron; D007930:Leucine