Digoxin radioimmunoassay: a comparison of the assay performances using tritiated and 125I radioiodinated tracers.
The preparation of 125I-labelled tracer for digoxin radioimmunoassay (125I monoiodinated 3-succinyl digoxigenin-1-tyrosine) is described, and its performance in radioimmunoassay of plasma samples is compared with that obtained with tritiated digoxin. The accuracy levels were assessed through the evaluation of different potential sources of systematic errors, such as interference from digoxin-related molecules and plasma proteins and methodological artefacts possibly associated with the immunocomplex instability, and through a series of checks including the recovery and parallelism tests and the correspondence of results obtained with the two tracers. The slope and the repeatability with time of the calibration curves and the spread of replicate estimates were taken into consideration to assess the assay precision. An essential equivalence in terms of reliability of measurement was proved for the two methodological variants, so that practical aspects and economic factors remain the main criteria to evaluate the relative merits: from this point of view, the advantages of using 125I-labelled tracer, as an alternative to tritiated digoxin, are discussed.
D004077:Digoxin / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D011863:Radioimmunoassay / Q000379:methods; D014316:Tritium
D007457:Iodine Radioisotopes; D014316:Tritium; D004077:Digoxin