A modified procedure for determination of metallothionein-like proteins in animal tissues.
1. The effect of low-molecular-weight substances interfering with determination of metallothionein is eliminated by precipitation of 203Hg-thionein by tannic acid. 2. In animals not exposed to metals, the mean values for metallothionein content in liver and kidney are, respectively, 0.10 and 0.18 mg/g in rats, 0.06 and 0.07 in mice and 0.12 and 0.29 in guinea-pigs, i.e. are 2-3 times lower as compared with the values obtained by the procedure not involving treatment with tannic acid.
D000818:Animals; D005590:Fractional Precipitation; D006168:Guinea Pigs; D047348:Hydrolyzable Tannins; D007668:Kidney / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008628:Mercury; D008667:Metalloproteins / Q000032:analysis*; D008668:Metallothionein / Q000032:analysis*; D051379:Mice; D051381:Rats; D013045:Species Specificity; D014238:Trichloroacetic Acid
D047348:Hydrolyzable Tannins; D008667:Metalloproteins; D014238:Trichloroacetic Acid; D008668:Metallothionein; D008628:Mercury