Effect of aflatoxin B1 on hepatic polyribosomes and protein synthesis in the rat.
This study was concerned with the effect of aflatoxin B1 on protein synthesis in rat liver. Specifically, the effect of the administration of aflatoxin B1 (6.0 mg/kg body weight) on hepatic polyribosomes (free and membranebound), protein synthesis in vitro, and initiation factors activity of rats within 3-12 h was investigated. The results revealed that aflatoxin B1 rapidly led to disaggregation of free and membrane-bound polyribosomes, to inhibition of in vitro protein synthesis by both populations of polyribosomes, to little or no effect on the activities of initiation factors, and to defective ribosomes, particularly the 60S ribosomal subnits, of both types of polyribosomes. Comparative studies on the effects of aflatoxin B1 and actinomycin D revealed progressive disaggregation by both hepatic free and membrane-bound polyribosomes after aflatoxin B1 but not only of the free polyribosomes after actinomycin D.
D000348:Aflatoxins / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D009963:Orotic Acid / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D010448:Peptide Initiation Factors / Q000494:pharmacology; D011132:Polyribosomes / Q000187:drug effects* / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014366:Tryptophan Oxygenase / Q000378:metabolism
D000348:Aflatoxins; D000596:Amino Acids; D010448:Peptide Initiation Factors; D003609:Dactinomycin; D009963:Orotic Acid; D014366:Tryptophan Oxygenase