Effect of testosterone and oestradiol on erythropoietin production in vitro.
The effects of testosterone and oestradiol on erythropoietin production by mouse fetal liver in primary culture was studied. No stimulatory effect on in vitro erythropoietin production was observed by any of the oestradiol concentrations employed [10(-10)M to 10(-6)M]: whereas testosterone, only over a dose range of 10(-12)M to 10(-10)M, stimulated erythropoietin production by mouse fetal liver in culture. In addition, a stimulatory effect of testosterone on fetal liver tissue was obtained by injecting testosterone (20 mg/mouse) intramuscularly into pregnant mice 4 d prior to obtaining the fetal liver tissue for culture. Therefore, it is concluded that testosterone can exert a direct stimulatory effect on erythropoietin production by mouse fetal liver.
D000818:Animals; D046508:Culture Techniques; D004921:Erythropoietin / Q000096:biosynthesis*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D051379:Mice; D011247:Pregnancy; D013268:Stimulation, Chemical; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology*
D004921:Erythropoietin; D013739:Testosterone; D004958:Estradiol