Determination of 2,4-diamino-5-(3,4-dichlorophenyl)-6-methylpyrimidine (BW 197U) in human plasma.
A gas-liquid chromatographic method for the measurement of 2,4-diamino-5-(3,4-dichlorophenyl)-6-methylpyrimidine (BW 197U) in human plasma has been developed. After extraction from alkaline medium into ethyl acetate the compound is injected into a gas-liquid chromatograph and measured using a 63Ni constant-current electron-capture detector. The range of concentrations measured was from 10 ng/ml to 10 microgram/ml in plasma. An internal standard was employed and reproducibility between replicates was found to be good. The method has been semi-automated by the use of an auto-sampler controlled by a minicomputer which also processes the data.
D000970:Antineoplastic Agents / Q000097:blood*; D001322:Autoanalysis; D002722:Chlorobenzenes / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods; D002853:Chromatography, Liquid; D003201:Computers; D006801:Humans; D008832:Microchemistry; D011743:Pyrimidines / Q000097:blood*
D000970:Antineoplastic Agents; D002722:Chlorobenzenes; D011743:Pyrimidines