Improved method for the analysis of estrogenic steroids in pregnancy urine by high-performance liquid chromatography.
The use of microparticulate packing materials and large injection volumes gives significant improvements in the analysis of complex samples, such as urine extracts by high-performance liquid chromatography. Lower detection limits and improved accuracy can now be attained. In addition, the combined use of adsorption and reversed-phase chromatography leads to reduced uncertainty in peak identification and gives more reliable quantification.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004958:Estradiol / Q000652:urine; D004964:Estriol / Q000652:urine; D004967:Estrogens / Q000652:urine*; D004970:Estrone / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy
D004967:Estrogens; D004970:Estrone; D004958:Estradiol; D004964:Estriol