Utilization of cyclodextrin complexation for separation of E, A, and B prostaglandins by ion-exchange liquid chromatography.
Application of cyclodextrin complexation to the separation of E-, A-, and B-type prostaglandins by ion-exchange liquid chromatography is demonstrated. The addition of alpha-or beta-cyclodextrin into the mobile phase on an anion-exchange support decreased the retention times of the prostaglandins significantly because of soluble complex formation. Chromatographic separation behavior is discussed on the basis of the stability of the inclusion complex. A rapid and sensitive method for the separation and quantification of the prostaglandins, using beta-cyclodextrin in the mobile phase, is described.
D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D003912:Dextrins; D007202:Indicators and Reagents; D008722:Methods; D011134:Polysaccharides; D011453:Prostaglandins / Q000302:isolation & purification*; D011454:Prostaglandins A / Q000302:isolation & purification*; D011458:Prostaglandins E / Q000302:isolation & purification*; D012995:Solubility
D003912:Dextrins; D007202:Indicators and Reagents; D011134:Polysaccharides; D011453:Prostaglandins; D011454:Prostaglandins A; D011458:Prostaglandins E