title: Effect of beta-adrenergic blockade, pertussis vaccine and Freund's adjuvant on reaginic antibody response in mice.
abstract: IgE synthesis has been reported to be inhibited by theophylline and dibutyryl cyclic adenosine monophosphate and increased by pertussis vaccine. This study was done to determine if chronic beta-adrenergic blockade affects IgE antibody production. 72 C57BL/10J mice were sensitized with egg albumin (EA, 125 microng). Groups included: (1) EA alone; (2) EA + complete Freund's adjuvant (FA); (3) EA + B. pertussis (8 X 10(9) organisms); (4) EA + propranolol (0.5 mg/kg) 3 times/day; (5) EA + FA + propranolol; (6) saline alone. Booster was given on day 49. Group 1, given EA alone, failed to develop any passive cutaneous anaphylaxis (PCA) antibodies even after booster, whereas the 72-hour PCA titers ranged from 1/64 to 1/256 for groups treated with propranolol and propranolol + FA, respectively. The results suggest that chronic beta-adrenergic blockade enhances IgE antibody formation.
mesh_terms: D000818:Animals; D000941:Antigens; D005620:Freund's Adjuvant / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis; D010567:Pertussis Vaccine / Q000494:pharmacology*; D011433:Propranolol / Q000276:immunology / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology
chemical_list: D000941:Antigens; D010567:Pertussis Vaccine; D007073:Immunoglobulin E; D010047:Ovalbumin; D005620:Freund's Adjuvant; D011433:Propranolol