High-pressure chromatography of tobacco mosaic virus on Spheron gels.
High-pressure chromatography on Spheron 300 BTD and Spheron 1000 was used to separate tobacco mosaic virus from other components of an extract from infected tobacco leaves, including F1, a protein having a molecular weight of 0.58 X 10(6). Comparative experiments have shown that the virus is not adsorbed by the carrier and is eluted in the free volume of the column; the virus is free from contamination by vegetable-juice components and is regained quantitatively. The virus can be separated partially, but distinctly, from the F1 protein by using a column of microgranular Spheron 300. Polyhydric phenols in the vegetable extract are adsorbed on Spheron and eluted from the column after a delay.
D002850:Chromatography, Gel; D009698:Nucleoproteins / Q000302:isolation & purification; D010940:Plant Proteins / Q000302:isolation & purification; D014027:Tobacco Mosaic Virus / Q000302:isolation & purification*; D014764:Viral Proteins / Q000302:isolation & purification
D009698:Nucleoproteins; D010940:Plant Proteins; D014764:Viral Proteins