title: Creatine kinase isoenzyme activity in human placenta and in serum of women in labor.
abstract: Creatine kinase (EC 2.7.3.2) isoenzymes in extracts of human placenta and in serum from nonpregnant women and women in labor were separated on columns containing diethylaminoethyl-cellulose and assayed. The distribution of the isoenzymes in placenta (n = 10) was 80% BB (200 +/- 66 U/g (wet weight), 19% MM (49 +/- 30 U/g), and 1% MB (2.6 +/- 1.7 U/g); The geometric mean for the serum BB activity of the nonpregnant women (n = 50) was 0.6 +/- 1.5 U/liter, as compared to 3.0 +/- 1.4 U/liter for patients in labor who had normal deliveries (n = 92). The arithmetic mean for serum BB activity of labor patients with induced labor (n = 20), premature labor (n = 7), cesarian section (n = 6), or hypertension and pre-eclampsia (n = 6) did not differ significantly from the arithmetic mean BB activity for serum of labor patients with normal deliveries. However, the arithmetic mean serum BB activity of patients with stillbirths (n = 7) was significantly smaller than the arithmetic mean for normal labor patients.
mesh_terms: D002848:Chromatography, DEAE-Cellulose; D003402:Creatine Kinase / Q000097:blood / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood / Q000378:metabolism*; D007743:Labor, Obstetric; D008722:Methods; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
chemical_list: D007527:Isoenzymes; D003402:Creatine Kinase