Proficiency testing: purification of lactate dehydrogenase 1 and results of its use as a reference material in the New York State program.
Highly purified human lactate dehydrogenase 1 has been used in an interlaboratory evaluation and improvement progran in clinical chemistry in New York State since 1971. Although there are difficulties in determining and assigning the most nearly accurate values for test samples in the absence of a reference method and a reference material, we have minimized these difficulties by using human lactate dehydrogenase preparations purified as we describe here and suspended in the same matrix, and by utilizing "reference laboratories" that routinely are doing multiple assays to determine the most nearly accurate value. The lactate dehydrogenase used in the program is stable for longer than 1.5 years. Conversion factors were used to convert all results to U/liter at 30 degrees C. Review of the data for 1972-75 shows a marked improvement in the accuracy of virtually all methods used to determine this enzyme.
D002624:Chemistry, Clinical; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D004912:Erythrocytes / Q000201:enzymology; D006358:Hot Temperature; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000302:isolation & purification*; D007753:Laboratories / Q000592:standards; D007773:Lactates; D046911:Macromolecular Substances; D009518:New York; D011773:Pyruvates; D011786:Quality Control; D012016:Reference Values
D007527:Isoenzymes; D007773:Lactates; D046911:Macromolecular Substances; D011773:Pyruvates; D007770:L-Lactate Dehydrogenase