Factor VIII deficient plasma for laboratory tests prepared from normal plasma and a human antibody.
Plasma from a patient factor VIII inhibitor was mixed at several low concentrations with fresh normal plasma. The factor VIII deficient plasmas obtained after a short incubation period were used as substrate plasmas in the normal one-stage factor VIII assay method. Results obtained using 0.5-1% of the inhibitor plasma in normal plasma compared favourably with those obtained using normal haemophilia A factor VIII deficient plasma provided that tests were carried out without delay. A single plasma exchange of this patient provided enough antibody to prepare more than 500 litres of factor VIII deficient plasma by simple mixing with fresh normal plasma.
D000328:Adult; D000906:Antibodies; D001780:Blood Coagulation Tests; D005169:Factor VIII; D006467:Hemophilia A / Q000175:diagnosis; D006801:Humans; D008297:Male; D010949:Plasma; D013925:Thromboplastin / Q000032:analysis; D013997:Time Factors
D000906:Antibodies; D005169:Factor VIII; D013925:Thromboplastin