[Lipoprotein analysis by means of the fluorescence probe 8-anilino-1-naphthalene sulphonic acid (author's transl)].
Single and mixed lipoprotein fractions were successfully analyzed by utilizing the fluorescent propertby of the applied reagent, 8-anilino-naphthalene sulphonic acid. The described method is especially useful for determination of lipoproteins present in high dilution, as found in chromatographic column effluents. The concentration dependence of the developed fluorescence was determined and this served as basis for quantitation. Furthermore, the measured activation spectra show two peaks for each lipoprotein class, the relative intesities being different for each class. The intensity quotient shows a tendency towards linear concentration dependence. Investigation of binary and ternary mixtures showed that the ratio of the two maximum intensities is additive, in contrast to the intensities themselves.
D006801:Humans; D007202:Indicators and Reagents; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D008297:Male; D009282:Naphthalenesulfonates; D013050:Spectrometry, Fluorescence / Q000379:methods
D007202:Indicators and Reagents; D008074:Lipoproteins; D009282:Naphthalenesulfonates
Analytische Anwendungen von Fluoreszenzmessung mit 8-Anilino-1-naphthalinsulfonsäure an Lipoproteinen.