Modified electron-capture GLC assay for salsolinol in brain tissue.
A modified assay for a neuroamine-derived tetrahydroisoquinoline, salsolinol, is presented. It combines the ease and rapidity of solvent extraction from brain tiessue with the sensitivity of electron-capture GLC. Detection of salsolinol (1,2,3,4-tetrahydro-1-methyl-6,7-isoquinolinediol), the alkaloid derived from condensation of dopamine and acetaldehyde, at levels of 5-10 ng/g of brain tissue is possible. The advantages afforded by the modifications are discussed in relation to existing procedures.
D000470:Alkaloids / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002849:Chromatography, Gas; D004298:Dopamine / Q000032:analysis; D007546:Isoquinolines / Q000032:analysis*; D008722:Methods; D051379:Mice; D014439:Tyramine / Q000032:analysis
D000470:Alkaloids; D007546:Isoquinolines; D004298:Dopamine; D014439:Tyramine