Enzymatic synthesis of adenosine 5'-O-(3-[35S]-thiotriphosphate).
A method for the enzymatic synthesis of adenosine 5'-O-(3-[35S]thiotriphosphate) is described. The method involves the thiophosphate exchange of adenosine 5'-O-(3-thiotriphosphate) with [35S]thiophosphate with the aid of glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase in the presence of 3-phosphoglycerate. The method is also applicable for the synthesis of guanosine 5'-O-(3-[35S]thiotriphosphate).
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000096:biosynthesis; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000096:biosynthesis; D007553:Isotope Labeling; D008274:Magnesium / Q000494:pharmacology; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D013462:Sulfur Radioisotopes
D005994:Glycerophosphates; D013462:Sulfur Radioisotopes; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010735:Phosphoglycerate Kinase; D008274:Magnesium