Non-specific cytotoxicity of spleen cells in mice bearing transplanted chemically induced fibrosarcomas.
Spleen cells collected from mice bearing transplanted chemically induced syngeneic fibrosarcomas non-specifically inhibited DNA synthesis of sarcoma and lymphoma target cells in vitro. Splenocytes from mice hyper-immunized against a syngeneic sarcoma specifically inhibited DNA synthesis of the tumour used for immunization. The impairment of tumour-cell DNA synthesis was associated in vitro with cytostasis, and lysis of the target cells was not seen. Since treatment with anti-theta serum and complement did not impair cytostatic action of the spleen cells, and since thymus-deprived animals showed similar activity to normal mice, T lymphocytes were not involved in non-specific cytostasis. Removal of phagocytic adherent cells by carbonyl iron markedly inhibited the cytostatic activity of the spleen cells, suggesting a role in this reaction for cells of the monocyte-macrophage series. The presence of an actively growing sarcoma was a prerequisite for the expression of non-specific cytostasis, since surgical excision resulted in complete disappearance of this activity of spleen cells.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D004247:DNA / Q000096:biosynthesis; D005354:Fibrosarcoma / Q000139:chemically induced / Q000276:immunology*; D007111:Immunity, Cellular; D008223:Lymphoma / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology; D012513:Sarcoma, Experimental / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000276:immunology*; D014185:Transplantation, Isogeneic
D004247:DNA