The influence of the serum/PHA ratio, microplate well shape and 2-mercaptoethanol on the stimulation of different numbers of cells in lymphocyte cultures from the chinese hamster.
Conditions for microculture of Chinese hamster lymphocytes are described which allow measurement of thymidine uptake with 6000 to 1000 lymphocytes per culture. The relationship between degree of cell stimulation, PHA concentration culture surface and cell concentration is described, as well as the influence of addition of 2-mercaptoethanol to the cultures.
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003545:Cysteine / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D005978:Glutathione / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008623:Mercaptoethanol / Q000494:pharmacology*
D037102:Lectins; D008623:Mercaptoethanol; D005978:Glutathione; D003545:Cysteine