Erythropoietic precursors in murine blood.
Murine blood was examined for circulating erythropoietic precursors. Blood mononuclear cells harvested using a slight modification of the Ficoll-Hypaque technique were cultured using the methylcellulose clonal cell culture technique. Only erythropoietic burst forming units (BFU-E) were present in the blood. Erythropoietin dose response studies revealed a progressive increment in the number of erythropoietic bursts up to a concentration of 4 U/ml of sheep plasma erythropoietin. A linear correlation existed between the numbers of nucleated cells plated and the erythropoietic bursts for both normal mice and mice with phenylhydrazine-induced (PHZ) anemia. No individual erythrocytic colonies were detected in cultures from blood taken before or after PHZ injections. These results suggest that erythrocytic colony-forming units (CFU-E) do not participate in the migration of erythropoietic precursors in mice.
D000818:Animals; D002999:Clone Cells; D004305:Dose-Response Relationship, Drug; D004920:Erythropoiesis / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D006412:Hematopoietic Stem Cells; D006461:Hemolysis / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes; D010659:Phenylhydrazines / Q000494:pharmacology; D013268:Stimulation, Chemical
D010659:Phenylhydrazines; D004921:Erythropoietin