title: The simultaneous quantitative determination of cephalothin and cefazolin in serum by high pressure liquid chromatography.
abstract: Conventional microbiological assay procedures for cephalosporins in serum do not allow the determination of serum concentrations if more than one cephalosporin is present in a single sample. An HPLC procedure has been developed which permits the simultaneous quantitative determination of cefazolin sodium and cephalothin sodium in serum. Reverse phase chromatography using methanol in 0.2 M ammonium acetate as the mobile phase was employed to separate and quantitate the two cephalosporins in a trichloroacetic acid supernatant solution prepared from serum.
mesh_terms: D002437:Cefazolin / Q000097:blood*; D002511:Cephalosporins / Q000097:blood*; D002512:Cephalothin / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008832:Microchemistry
chemical_list: D002511:Cephalosporins; D002437:Cefazolin; D002512:Cephalothin