Evidence for a cytosol counterpart of the major plasma membrane protein in Acanthamoeba castellanii.
Isolated plasma membranes from Acanthamoeba castellanii incorporated radio-activity when incubated in a corresponding labelled cytosol fraction. The incorporation increased linearly with time and reached saturation at high cytosol: membrane protein ratios in the incubation mixture. Polyacrylamide gel electrophoresis of treated membranes showed that the major protein, which comprised 40 to 50% of the total, was labelled, suggesting that it had exchanged with a radioactive cytosol counterpart. The same protein was discernible in polypeptide profiles of labelled cytosol and showed a higher specific radioactie membrane by solutions of high ionic strength or chelation, this protein appears to be integral rather than peripheral.
D000656:Amoeba / Q000648:ultrastructure*; D000818:Animals; D003600:Cytosol / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis*; D011506:Proteins / Q000032:analysis
D008565:Membrane Proteins; D011506:Proteins