Biologically active macromolecular forms of oxytocin. [8-Lysine]oxytocin as a suitable ligand.
[8-Lysine]oxytocin was synthesized on a solid support and possessed an oxytocic activity of 100 +/- 6 units mumol on the isolated rat uterus. The epsilon-carbamoyl, epsilon-3-carboxypropionyl and epsilon-3-carboxybutryl derivatives were prepared and had uterotonic activities of 400, 55 and 50 units/mumol respectively. [8-Lysine]oxytocin was coupled unambiguously through the epsilon-amino group to the carboxyl groups of carboxymethylated dextrans or epsilon-3-carboxypropionly-gelatin. The macromolecular oxytocins were water-soluble and retained signigicant oxytocic activity. [8-Lysine]oxytocin should prove a useful ligand for affinity chromatography of oxytocin-binding proteins.
D000818:Animals; D003911:Dextrans / Q000031:analogs & derivatives; D005260:Female; D005780:Gelatin / Q000031:analogs & derivatives; D008239:Lysine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000494:pharmacology; D046911:Macromolecular Substances; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D051381:Rats; D014599:Uterus / Q000187:drug effects
D003911:Dextrans; D046911:Macromolecular Substances; D010121:Oxytocin; D005780:Gelatin; D008239:Lysine