Biochemical analysis of meiosis in the male mouse. I. Separation of DNA labelling of specific spermatogenic stages.
Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of semi-conservative meiotic DNA synthesis, essential to the biochemical analysis of pachytene DNA metabolism, has been achieved by in vivo administration of 1 M hydroxyurea.
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D006918:Hydroxyurea / Q000494:pharmacology; D008297:Male; D008540:Meiosis; D051379:Mice / Q000033:anatomy & histology*; D013091:Spermatogenesis; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D004247:DNA; D013936:Thymidine; D006918:Hydroxyurea