Toxic effect of water-soluble fractions of crude, refined, and weathered oils on the growth of a marine bacterium.
The water-soluble fractions of three crude and two refined oils reduced the growth rate and maximum cell density of the marine bacterium Serratia marinorubra grown in batch culture. The weathering of a crude and a refined oil was simulated in the laboratory. The water-soluble fractions remaining from this process were more toxic to S. marinorubra than were the parent unweathered oils. Increases in the magnitude of toxic effect of 3 to 30 times were observed as a function of decreasing the concentration of yeast extract in the cultures from 0.1 to 0.05 and 0.01%. The toxicity did not correlate with the concentration of total water-soluble fraction or of aromatic hydrocarbons in the water-soluble fraction. Affected cultures did not exhibit a residual toxicity after being back-inoculated into control media.
D005648:Fuel Oils; D006838:Hydrocarbons / Q000494:pharmacology; D010578:Petroleum; D012623:Seawater; D012705:Serratia / Q000187:drug effects / Q000254:growth & development*; D012995:Solubility; D014871:Water Microbiology
D005648:Fuel Oils; D006838:Hydrocarbons; D010578:Petroleum