title: Development of Microphalloides japonicus (Osborn, 1919) metacercariae in vitro (Trematoda: Microphallidae).
abstract: Metacercariae of the trematode Microphalloides japonicus (Osborn, 1919) were cultivated in various media to gravid adults, and the results were compared with those in vivo. A few eggs were produced in the uterus even in balanced salt solution, although cultivation in Eagle's MEM and NCTC 109 media was much better. Addition of heat-inactivated serum resulted in even better development. Encysted metacercariae also produced eggs in some media, but most of them were incomplete and without shells.
mesh_terms: D000818:Animals; D003386:Brachyura / Q000469:parasitology; D003470:Culture Media; D014200:Trematoda / Q000033:anatomy & histology / Q000254:growth & development*
chemical_list: D003470:Culture Media