Methylazoxymethanol-induced aberrant Purkinje cell dendritic development.
Purkinje cell dendrites develop with a specific orientation and relationship to related neurons and glia. Their dendritic spine postsynaptic membrane specialization may, in turn, require a permanent presynaptic contact by parallel fibers. To determine whether changes in the surrounding cells influence the normal development of the cerebellar Purkinje cell dendrites and spine specializations, destruction on the differentiating cell layer was induced in the postnatal mouse by administration of methylazoxymethanol acetate (MAM) (0.05 micronl/gm body weight) at day zero. The Purkinje cells were examined by light and electron microscopy on the tenth postnatal day. The midsagittal surface area of the cerebellar vermis in treated animals was reduced by an average of 60%. MAM-induced granule cell depletion and Purkinje cell dislocation were observed by light microscopy. When compared to controls, examination following Golgi impregnation revealed random orientation of Purkinje cell apical poles, and multiple primary dendrites of reduced length with few branches, branchlets and spines. Vertical processes of Golgi epithelial (Bergmann) cells were obliquely directed, reduced in length and complexity in MAM-treated mice. Ultrastructural examination revealed naked Purkinje cell dendritic spine specializations in both groups. Although necrotic debris persisted in astrocytes and macrophages, degenerating presynaptic terminals were not found. This study suggests that permanent presynaptic contact by parallel fibers is not essential for spine development. Astrocytic reactions to injury, in association with the reduced folial expansion, may have contributed to the observed abnormalities and disorientation of the Purkinje cells. The data suggests that Purkinje cell dendritic development may be strongly influenced by changes in surrounding cells.
D000818:Animals; D001253:Astrocytes / Q000648:ultrastructure; D001391:Azo Compounds / Q000633:toxicity*; D002526:Cerebellar Diseases / Q000139:chemically induced; D003712:Dendrites / Q000187:drug effects* / Q000648:ultrastructure; D005260:Female; D008297:Male; D008746:Methylazoxymethanol Acetate / Q000633:toxicity*; D051379:Mice; D011689:Purkinje Cells / Q000187:drug effects* / Q000648:ultrastructure; D013569:Synapses / Q000648:ultrastructure
D001391:Azo Compounds; D008746:Methylazoxymethanol Acetate