Enzyme-coupled measurement of uric acid in serum with a centrifugal analyzer.
An early-reading blank-corrected end-point determination of uric acid in serum has been developed for use with a centrifugal analyzer. The method is based on a modification of the uricase (urate:oxygen oxidoreductase, EC 1.7.3.3)/catalase (hydrogen peroxide:hydrogen peroxide oxidoreductase EC 1.11.1.6)/aldehyde dehydrogenase (aldehyde:NAD(P)+ oxidoreductase, EC 1.2.1.5)-coupled analytical scheme reported by Haeckel [Z. Klin. Chem. Klin. Biochem. 14, 101 (1976)]. Sensitivity and precision of the method are excellent, and results compare well with those obtained by the Kageyama procedure [Clin. Chim. Acta 31, 421 (1971)].
D000445:Aldehyde Oxidoreductases; D001322:Autoanalysis; D002498:Centrifugation; D006801:Humans; D008722:Methods; D014503:Urate Oxidase; D014527:Uric Acid / Q000097:blood*
D014527:Uric Acid; D000445:Aldehyde Oxidoreductases; D014503:Urate Oxidase