Methylation analysis of neuraminic acids by gas chromatography-mass spectrometry.
The permethylated methyl glycoside methyl esters of N-acetyl- and N-glycolyl-neuraminic acids and of N-acetylneuraminic acid 8-acetate have been analyzed by g.1.c.-mass spectrometry. Fragmentation of the neuraminic acid derivatives in electron-impact mass spectrometry has been studied by deuterium labelling. The results were applied in the methylation analysis of neuraminic acids from gangliosides of brain and kidney.
D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008745:Methylation; D012794:Sialic Acids / Q000032:analysis*
D012794:Sialic Acids