Studies on Bermuda grass pollen allergens.
Two allergens, BGP-1 and BGP-2, were identified in crude Bermuda grass pollen extract. BGP-1 was a major allergen and elicited a skin reaction in all subjects sensitive to the crude extract, whereas BGP-2 was a minor allergen and elicited a reaction in only 75% of such subjects. Calibrated column chromatography was used to determine the molecular weights and Stokes radii of the allergens. For BGP-1 they were 30 000 daltons and 24,3 X 10(-8) cm respectively and for BGP-2 they were 14 000 daltons and 18,1 X 10(-8) respectively. The sedimentation coefficient (S20,w) of BGP-1 on sucrose density gradient ultracentrifugation was 3,1 and on iso-electric focusing its iso-electric point was found to be pH 5,4. The reason why only certain individuals sensitive to BGP-1 are sensitive to BGP-2 is discussed. It is possible that the former is a dimer of the latter.
D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D006801:Humans; D007526:Isoelectric Point; D008970:Molecular Weight; D010936:Plant Extracts / Q000032:analysis*; D006109:Poaceae / Q000032:analysis; D011058:Pollen / Q000032:analysis*; D012130:Respiratory Hypersensitivity / Q000175:diagnosis; D012882:Skin Tests; D014461:Ultracentrifugation
D010936:Plant Extracts