5-Hydroxytryptamine binding to butanol extracts from myelin fragments.
The myelin fraction of rat brain stem was treated with butanol-water mixtures, and the extracted proteolipids were separated by Sephadex LH20 column chromatography. 2 peaks of proteolipids eluted in chloroform-methanol 4/1 showed the binding capacity for C14 - 5-HT. This finding suggests the necessity of the more careful investigations for the probability of proteolipids as receptor proteins in the central nervous system.
D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D000440:Butanols; D008297:Male; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000378:metabolism* / Q000648:ultrastructure; D011510:Proteolipids / Q000378:metabolism; D051381:Rats; D011985:Receptors, Serotonin / Q000302:isolation & purification / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*
D000440:Butanols; D011510:Proteolipids; D011985:Receptors, Serotonin; D012701:Serotonin