Investigation of peptides containing half-cystine in pig pancreas amylase.
Sequence analysis of the carboxymethyl-cystein-containing tryptic peptides isolated after the total reduction and carboxymethylation of pig pancreas amylase has shown that the half-cystine-containing tryptic peptides of the isozymes have identical sequences. The fact that 10 tryptic peptides containing carboxymethyl-cystein could be isolated, supports the generally accepted view that pig pancreas amylase contains single polypeptide chain.
D000595:Amino Acid Sequence; D000681:Amylases / Q000032:analysis*; D000818:Animals; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000032:analysis; D010179:Pancreas / Q000201:enzymology*; D010455:Peptides / Q000032:analysis*; D013552:Swine
D007527:Isoenzymes; D010455:Peptides; D000681:Amylases