A serological investigation into the acidic alpha-D-mannosidase in normal Angus cattle and in a calf with mannosidosis.
Antiserum was raised in a rabbit against bovine kidney acidic alpha-mannosidase that had been purified 570-fold by affinity chromatography on concanavalin A--Sepharose and Sepharose 4B-xi-aminohexanoylmannosylamine. The antiserum precipitated the acidic but not the neutral alpha-mannosidase in normal calf tissues. Human acidic alpha-mannosidase cross-reacted partially with the antiserum, emphasizing the close structural resemblance between the enzyme in the two species. The residual acidic alpha-mannosidase in the tissues of a calf with mannosidosis was also precipitated by the antiserum, the same volume of antiserum being required to precipitate a unit of alpha-mannosidase activity from the normal and pathological tissues. The concentration of cross-reacting material detected by antibody-consumption experiments in the organs of the calf with mannosidosis appeared to be proportional to the concentration of the residual acidic alpha-mannosidase. It is suggested that the residual acidic alpha-mannosidase in mannosidosis accounts for the cross-reacting material detected and that it is unlikely that enzymically inactive but cross-reacting material is present. The residual acidic alpha-mannosidase could be a decreased concentration of the normal gene product or an altered enzyme with a decreased specific enzymic activity and a correspondingly decreased antigenicity.
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002239:Carbohydrate Metabolism, Inborn Errors / Q000378:metabolism / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000201:enzymology*; D003429:Cross Reactions; D004186:Disaccharidases / Q000172:deficiency*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008361:Mannosidases / Q000172:deficiency* / Q000276:immunology
D004186:Disaccharidases; D008361:Mannosidases