Replication process of the parvovirus H-1. IX. Physical mapping studies of the H-1 genome.
The cleavage map of H-1 replicative-form DNA to the bacterial restriction endonuclease EcoRI, HaeII, HaeIII, HindII, HindIII, and HpaII has been determined. The 5'-phosphoryl end of the viral strand is on the right end of the molecule at or near the replication origin. Evidence is presented for the presence of inverted self-complementary sequences at the right end that differ from those at the left end. These sequences allow a foldback of the DNA after denaturation, and a minority of the native replicative-form DNA has the foldback configuration. The possible role of these structures in H-1 DNA synthesis is discussed.
D001483:Base Sequence; D002874:Chromosome Mapping; D046508:Culture Techniques; D004261:DNA Replication; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D010321:Parvoviridae / Q000032:analysis / Q000378:metabolism*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes