Extraction procedures for use prior to HPLC nucleotide analysis using microparticle chemically bonded packings.
In preparing nucleotide extracts for HPLC analysis using microparticle, chemically bonded packings, tri-octyl-amine-freon solutions can be used for the extraction of the acid from the solution if the concentration of the amine is carefully controlled.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009711:Nucleotides / Q000097:blood*
D009711:Nucleotides