title: Regulation of growth and DNA synthesis in mouse lymphoma L5178Y cells under conditions of nutrient depletion.
abstract: The effect of cultivation of L5178Y lymphoblasts to high density and in two kinds of nutrient-depleted medium on their growth was observed. It was found that in cell population grown to stationary phase the mitotic index and labeled thymidine incorporation dropped about 3 times in comparison with exponential control. Upon dilution with fresh medium the cells resume traverse the life cycle in partially synchronized manner. Cultivation of the cells in medium with 0-5% serum induced very rapid loss of their viability. Cultivation of the cells in medium without asparagine and with dialyzed serum caused significant slowing of their growth-rate and upon dilution with complete medium the cells entered the exponential rate of division in an asynchronous manner.
mesh_terms: D001769:Blood; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D003260:Contact Inhibition; D003470:Culture Media; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D008223:Lymphoma / Q000378:metabolism / Q000503:physiopathology*; D008938:Mitosis; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000503:physiopathology; D009747:Nutritional Physiological Phenomena
chemical_list: D003470:Culture Media; D004273:DNA, Neoplasm