Kinetics of Mu DNA synthesis.
Mu specific DNA synthesis starts at 10 min after infection. All essentail amber mutants of Mu were tested for the ability to replicate in a non permissive host. Except for the amber mutants A and B, which were already known to be blocked in Mu DNA synthesis (Wijffelman et al., 1974), all the other mutants showed normal Mu DNA replication. Using mitomycin C-treated cells Mu DNA synthesis was found to start at about 20 min after induction. However using the much more sensitive method of DNA-RNA hybridization, it was found that the DNA synthesis starts already at 10 min after induction, and that at 20 min after induction about 7 copies of the Mu DNA are present per cell.
D003090:Coliphages / Q000187:drug effects / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis*; D007700:Kinetics; D008242:Lysogeny; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral; D008937:Mitomycins