Study on protein content of rabbit reticulocyte ribosomal subunits: correlation with functional activity.
The correlation between chemical composition (protein content) of native and derived ribosomal subunits and their activity in a cell-free system, programmed with haemoglobin messenger RNA, has been investigated. On the basis of isodensity sedimentation of formaldehyde fixed particles in CsC1 and of two-dimensional gel electrophoresis of ribosomal proteins, it appears that the native ribosomal subparticles contain a large protein complement than the derived subparticles and, correspondingly, they are less dependent on an external supply of initiation factors for haemoglobin synthesis in vitro.
D000818:Animals; D002474:Cell-Free System; D005914:Globins / Q000096:biosynthesis*; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010649:Phenylalanine / Q000378:metabolism; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000097:blood*
D012333:RNA, Messenger; D012269:Ribosomal Proteins; D010649:Phenylalanine; D011691:Puromycin; D005914:Globins