title: Measurement of 1-aspartamido-beta-N-acetylglucosamine amidohydrolase activity in human tissues.
abstract: The activity of 1-aspartamido-beta-N-acetylglucosamine amidohydrolase (aspartylglucosylaminase, EC 3.5.1.26) was measured in normal and diseased human liver, brain and kidney. Organs from patients with aspartylglucosaminuria show very little activity. Crude homogenates of human organs show a reaction catalysed by a complex enzyme system. With homogenate, the formation of product was linear with time up to about 6 h. Reaction times longer than 6-7h resulted in a decrease in the total concentration of product. This phenomenon was not found with the partially purified enzyme fraction. Linearity of the enzyme activity with different protein concentrations was found, independent of the incubation time. Longer incubation of the crude homogenate resulted in the utilization of the product, N-acetylglucosamine. This phenomenon was not observed with the partially purified enzyme fraction. This amidase from human organs differs from that obtained from other sources and apparently represents a rather complex enzyme system.
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D000581:Amidohydrolases / Q000378:metabolism*; D001227:Aspartylglucosylaminase / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D006801:Humans; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D011506:Proteins / Q000378:metabolism
chemical_list: D011506:Proteins; D000581:Amidohydrolases; D001227:Aspartylglucosylaminase; D000117:Acetylglucosamine