A rapid procedure for selectively isolating the major glycoprotein from purified rat brain myelin.
Treatment of the chloroform/methanol-insoluble residue of rat brain myelin with lithium 3,5-di-iodosalicylate solubilized the major myelin-associated glycoprotein along with most other proteins and glycoproteins. Equilibration of the extract with phenol resulted in the selective partitioning of the major glycoprotein into the aqueous phase, whereas nearly all of the other proteins and glycoproteins went into the phenol phase.
D000818:Animals; D001923:Brain Chemistry; D006023:Glycoproteins / Q000302:isolation & purification*; D008094:Lithium; D008722:Methods; D009185:Myelin Proteins / Q000302:isolation & purification*; D051381:Rats; D012459:Salicylates; D012995:Solubility
D006023:Glycoproteins; D009185:Myelin Proteins; D012459:Salicylates; D008094:Lithium