Blood storage XXIII: 2,3-DPG maintenance for six weeks in a CPD-adenine-inosine preservative with and without methylene blue.
In a pilot study the optimal concentration of inosine for 2,3-DPG maintenance in a CPD-adenine (0.25 mM) preservative was confirmed to be at lease 10 mM. In these experiments, 2,3-DPG maintenance was nearly normal for six weeks of storage in CPD-adenine-inosine (10 mM) preservative with or without methylene blue. The control preservative lacking inosine showed a statistically significant decrease in 2,3-DPG concentrations after the 3rd week. Finally, 2,3-DPG levels were significantly better maintained in CPD-adenine preservatives that contained 15 mM concentrations of inosine, whether methylene blue was present or not (10(-6)M), compared to CPD-adenine-inosine preservatives that contained 5 mM inosine, with or without methylene blue. The methylene blue effect, while it can be demonstrated in most experiments to help the red blood cell maintain 2,3-DPG during prolonged blood storage, is judges to be a slight value. However, inosine is of great value in maintaining 2,3-DPG for prolonged (five to six weeks) liquid storage.
D000225:Adenine; D000255:Adenosine Triphosphate / Q000032:analysis; D000704:Analysis of Variance; D001793:Blood Preservation / Q000379:methods / Q000592:standards*; D002951:Citrates; D004163:Diphosphoglyceric Acids / Q000032:analysis*; D005947:Glucose; D006801:Humans; D007288:Inosine; D008751:Methylene Blue; D010710:Phosphates
D002951:Citrates; D004163:Diphosphoglyceric Acids; D010710:Phosphates; D007288:Inosine; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine; D008751:Methylene Blue