title: Evaluation of two commercial kits for serum gastrin assay, and comparison with a conventional radioimmunoassay procedure.
abstract: We compared two gastrin radioimmunoassay kits ("Immutope" kit, Squibb & Co.; "Gastrin R.I.A." kit, Schwarz/Mann) to the conventional gastrin radioimmunoassay of Yalow and Berson [Gastroenterology 58, 1 (1970)] as run by us and by a second reference laboratory. Although both kits were found to effectively discriminate above-normal and normal values for serum gastrin, they significantly underestimated very high values (greater than 1500 ng/liter). The Schwarz/Mann kit clearly had a superior quality label (lower nonspecific binding and higher specific activity) and a shorter incubation time. However, the 90-min incubation period cited for their kit caused overestimation of gastrin values in the lower range (5-300 ng/liter), which could be corrected by prolonging the incubation to 24 h. The Squibb antibody had fairly good cross reactivity to all gastrin species tested; the Schwarz/Mann antibody had poor affinity for natural human gastrin G34-II. Good correspondence was found for sera run by both reference laboratories (y = 0.96x + 10, r = 0.997), and values obtained with the Schwarz/Mann kit correlated best (+ 0.815) with those from the conventional radioimmunoassay procedure.
mesh_terms: D000918:Antibody Specificity; D002021:Buffers; D005069:Evaluation Studies as Topic; D005755:Gastrins / Q000097:blood* / Q000276:immunology; D006801:Humans; D008722:Methods; D011863:Radioimmunoassay; D012016:Reference Values; D013997:Time Factors
chemical_list: D002021:Buffers; D005755:Gastrins