title: Binding of [125I]concanavalin A by interspecific amoeba hybrids.
abstract: Two types of interspecific amoeba hybrids, viz. PnIc (Amoeba proteus nucleus in A. indica cytoplasm) and InPc (A. indica nucleus in A. proteus cytoplasm), were tested for their [125I]Concanavalin A (Con A) binding activity at different periods of time. The cell surface binding of the labelled Con A was reduced to approx. 40 and 75% in the PnIc and InPc cells, respectively, 96 h after the cells were made hybrids. A significant increase in the binding of radioactive Con A was observed after the homologous nucleus was back transferred into InPc cells, but not in PnIc cells. No significant difference in the binding of the radioactive Con A was found between the progeny of the hybrid amoebae and the control amoebae. The Con A binding activity of the interspecific amoebae has been discussed in relation to the changes in the cytoplasmic membrane structures which presumably play a role in the formation of cell surface in the amoebae.
mesh_terms: D000656:Amoeba / Q000378:metabolism*; D000818:Animals; D003208:Concanavalin A / Q000378:metabolism*; D006824:Hybridization, Genetic; D007457:Iodine Radioisotopes; D008766:Methylmannosides / Q000494:pharmacology; D011952:Receptors, Concanavalin A / Q000187:drug effects; D011955:Receptors, Drug / Q000187:drug effects
chemical_list: D007457:Iodine Radioisotopes; D008766:Methylmannosides; D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D003208:Concanavalin A