title: Circular dichroism of insulin A chain in water: trifluoroethanol mixtures. Use of linear and nonlinear least squares analysis.
abstract: Circular dichroic spectra obtained for S-carboxymethylated insulin A chain in water: trifluoroethanol mixtures show that a marked conformational transition occurs as the concentration of trifluoroethanol is increased to (v/v) 83% to give a polypeptide containing about 43% of the residues in the alph-helical conformation. Several proposed methods of analysis, including both linear and two nonlinear lest squares methods, were unable to quantitate the amount of beta-structure present in the polypeptide in 83% trifluoroethanol. Examination of the methods of analysis lead to the conclusion that the current models for far UV CD analysis are not adequare for the data obtained in this study and shows that nonlinear least squares procedures may lead to erroneous conclusions.
mesh_terms: D002942:Circular Dichroism; D003201:Computers; D007328:Insulin; D008433:Mathematics; D011487:Protein Conformation; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013223:Statistics as Topic
chemical_list: D007328:Insulin; D012997:Solvents