Evaluation of a microspectrofluorimetric method or serum vitamin A.
A microspectrofluorimetric method for vitamin A was compared with spectrophotometric and trifluoroacetic acid-colorimetric procedures. Values obtained by the fluorimetric method showed a high degree of correlation with those obtained by other standard procedures. However, it was found that the fluorimetric method yielded values which were several-fold higher than those obtained with the spectrophotometric method when sera from pregnant women were tested. The fluorescence from sera of pregnant subjects showed a triphasic decline even when the samples were stored at --20 degrees C.
D002648:Child; D003124:Colorimetry; D004355:Drug Stability; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008722:Methods; D008832:Microchemistry; D011247:Pregnancy; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013997:Time Factors; D014801:Vitamin A / Q000097:blood*
D014801:Vitamin A