Norepinephrine uptake in guinea pig lung. Effects of anaphylaxis, phenoxybenzamine and cocaine.
Lungs from control and sensitized guinea pigs were perfused via the pulmonary artery with Tyrode's solution. Infusion of equimolar concentrations (3x10(-9) to 3x10(-6 M) of 3H-1-norepinephrine (NE) and 14C-urea was started 1 min after injection of 1 ml 0.9% NaCl or 50 mg ovalbumin in 1 ml 0.9% NaCl and continued for 15 min. Anaphylactic lungs retained more urea, NE, and NE metabolites than control lungs at all NE concentrations and the proportion of unchanged NE was higher. Phenoxybenzamine decreased the 3H/14C ratio due to decreased 3H accumulation. Cocaine affected predominantly the control lungs. The results indicate that anaphylaxis increased the net uptake of NE in the lung and decreased the metabolism of retained NE.
D000707:Anaphylaxis / Q000378:metabolism*; D000818:Animals; D000941:Antigens; D002463:Cell Membrane Permeability; D003042:Cocaine / Q000494:pharmacology*; D006168:Guinea Pigs; D007114:Immunization; D008168:Lung / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism*; D010047:Ovalbumin / Q000276:immunology; D010643:Phenoxybenzamine / Q000494:pharmacology*; D014508:Urea / Q000378:metabolism
D000941:Antigens; D010643:Phenoxybenzamine; D014508:Urea; D010047:Ovalbumin; D003042:Cocaine; D009638:Norepinephrine