title: Organometallic and organometalloid compounds as standards for microprobe analysis of epoxy resin embedded tissue.
abstract: X-ray microanalysis of phosphorus, transition elements and heavy metals in biological tissue is frequently carried out on thin sections of specimens embedded in epoxy resin. A logical choice for the quantitive microprobe analysis of these specimens is a standard, consisting of a homogenous solution of the elements of interest in the epoxy resin. Four kinds of compounds were found suitable  for this purpose: (1) phenyl compounds containing group Vb elements, (2) cyclopentadienyl-derivatives, (3) a pentanedione derivative (acetylacetonate) and (4) complexes of metals with dialkyldithiocarbamates. In the latter case, the standard also contains sulphur. Standards for P, Sb (1) Mn, Fe (2) Ni (3) Cu, Zn, Cd, Hg, Pb, Bi (4) were prepared in Epon 812 or Spurr epoxy resin. The compounds were mixed with the resin (without accelerator) to which some propylene oxide may be added, and dissolved immediately or after short heating. The maximal concentration of metal was in the order of magnitude of several promilles to 1%. Solubility in the Spurr resin was better than in Epon 812. After addition of the accelerator, polymerization was carried out as usual. The compounds used are commercially availalbe at low cost or can be easily prepared.
mesh_terms: D000473:Alkanes; D003517:Cyclopentanes; D004577:Electron Probe Microanalysis; D004853:Epoxy Resins; D009942:Organometallic Compounds; D010636:Phenols; D013859:Thiocarbamates; D014965:X-Rays
chemical_list: D000473:Alkanes; D003517:Cyclopentanes; D004853:Epoxy Resins; D009942:Organometallic Compounds; D010636:Phenols; D013859:Thiocarbamates