Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides synthesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in otheruman cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 250 K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.
D000260:Adenoviruses, Human / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004279:DNA, Viral / Q000096:biosynthesis; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D010452:Peptide Biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D004279:DNA, Viral; D006023:Glycoproteins; D014764:Viral Proteins