GLC determination of acetohexamide and hydroxyhexamide in biological fluids.
A sensitive and specific GLC assay was developed for acetohexamide and hydroxyhexamide, its major metabolite, in plasma and urine. The assay uses tolbutamide as a mass internal standard. Compounds are extracted from acidified plasma or urine with toluene, converted to methylated derivatives with dimethyl sulfate, and measured by GLC using a flame-ionization detector. With GLC-mass spectrometry, the compounds measured are the N-methylsulfonamides resulting from GLC pyrolysis. Plasma and urine data are presented from a bioavailability study to demonstrate the utility of this method.
D000092:Acetohexamide / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D001682:Biological Availability; D001786:Blood Glucose / Q000032:analysis; D002849:Chromatography, Gas; D006801:Humans; D007328:Insulin / Q000097:blood; D013058:Mass Spectrometry; D008722:Methods; D013607:Tablets; D013997:Time Factors
D001786:Blood Glucose; D007328:Insulin; D013607:Tablets; D000092:Acetohexamide