Actin from the nematode, Caenorhabditis elegans, is a single electrofocusing species.
We have prepared actin from wild type Caenorhabditis elegans animals by three procedures: a purification dependent on the ability of actin to form F-actin, affinity chromatography which preferentially binds G-actin, and co-precipitation of an actin-myosin complex by antimyosin antibodies. Each preparation yields a single electrofucsing species of actin. Comparison of actin from C. elegans embryos and animals reveals that embryos also have the same single electrofocusing species of actin.
D000199:Actins / Q000302:isolation & purification; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009348:Nematoda / Q000032:analysis*; D011817:Rabbits; D013045:Species Specificity
D000199:Actins