Serum-induced changes in electrophoretic mobility of creatine phosphokinase isoenzymes?
BB isoenzyme of creatine phosphokinase (CK-BB) obtained from human brain-extract changes its electrophoretic mobility after incubation in human serum at 37 degrees C. No change of electrophoretic mobility of CK-BB is observed after incubation in isotonic saline. We have shown by means of immunoprecipitation with specific antibodies that the structure of CK-BB is not changed. These findings are supported by other authors and make the diagnostic value of electrophoretic separation of CK isoenzymes doubtful as after a 3-h incubation CK-BB migrates similarly to CK-BB and consequently may be misinterpreted.
D001921:Brain / Q000201:enzymology; D003402:Creatine Kinase / Q000097:blood / Q000378:metabolism*; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007527:Isoenzymes / Q000097:blood / Q000378:metabolism*; D011233:Precipitin Tests
D007527:Isoenzymes; D003402:Creatine Kinase