A methionine-reversible folate deficiency in rats following the acute administration of diethylnitrosamine and alpha-naphthylisothiocyanate.
Acute doses of the hepatotoxic agents diethylnitrosamine (DEN) and alpha-naphthylisothiocyanate (ANIT) to young adult male rats led to the production of a folate deficiency as determined by an elevated urinary excretion of formiminoglutamic acid (FIGLU) 2 to 4 days following administration of the compounds. High dietary levels of methionine significantly reduced the elevated levels of FIGLU produced by the 2 chemicals. Dietary folate had no significant effect on the excretion of urinary FIGLU. Although the hepatic levels of S-adenosylmethionine (SAM) were significantly increased in rats fed the high dietary levels of methionine, an acute dose of DEN did not depress the hepatic levels of SAM. The results indicate that the methyl-reversible folate deficiency caused by hepatotoxic agents is not the direct consequence of altered hepatic levels of SAM.
D015058:1-Naphthylisothiocyanate / Q000008:administration & dosage / Q000009:adverse effects*; D000818:Animals; D002794:Choline / Q000494:pharmacology; D004032:Diet; D004052:Diethylnitrosamine / Q000008:administration & dosage / Q000009:adverse effects*; D005492:Folic Acid / Q000494:pharmacology; D005494:Folic Acid Deficiency / Q000139:chemically induced* / Q000188:drug therapy / Q000378:metabolism; D005565:Formiminoglutamic Acid / Q000652:urine; D008099:Liver / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000494:pharmacology* / Q000627:therapeutic use; D009602:Nitrosamines / Q000009:adverse effects*; D051381:Rats; D012436:S-Adenosylmethionine / Q000378:metabolism; D013861:Thiocyanates / Q000009:adverse effects*; D013997:Time Factors
D009602:Nitrosamines; D013861:Thiocyanates; D004052:Diethylnitrosamine; D015058:1-Naphthylisothiocyanate; D012436:S-Adenosylmethionine; D005565:Formiminoglutamic Acid; D005492:Folic Acid; D008715:Methionine; D002794:Choline