Granular cells in the connective tissue of Helix pomatia L. (gastropoda, pulmonata). Histochemistry, ultrastructure, and results of polyacrylamide electrophoretic investigations.
Granular cells (cells crowded with colourless granules staining with paraldehyde fuchsin according to Gomori-Gabe and not containing calcium) are independent cells in the connective tissue of Helix pomatia. Histochemical data suggest that the granules are rich in sulfhydryl-containing proteins, but lack biogenic monoamines. Electron microscopic investigations confirm the supposed secretory activity of the granular cells. Secretory proteins are presumed to be synthetized in the endoplasmic reticulum and condensed in the Golgi apparatus giving rise to the granules. Extrusion occurs by exocytosis. Electrophoresis of homogenates, prepared from tissues containing numerous granular cells, results in the separation and identification of a secretory protein from the granular cells. An electrophoretically homologous protein is recognized in the hemolymph, but in very small quantities. Our findings and the work of others suggest the involvement of granular cells in neuroendocrine events.
D000818:Animals; D002474:Cell-Free System; D003238:Connective Tissue / Q000378:metabolism / Q000648:ultrastructure*; D003239:Connective Tissue Cells; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum; D006056:Golgi Apparatus; D006372:Helix, Snails / Q000033:anatomy & histology*; D006458:Hemolymph / Q000032:analysis; D006651:Histocytochemistry; D008854:Microscopy, Electron; D011506:Proteins / Q000032:analysis; D012908:Snails / Q000033:anatomy & histology*
D011506:Proteins