Detection of methyltransferase activities which modify Gppp G to m7GpppGm in embryonic chick lens.
GpppG was modified to m7 GpppGm by a cytoplasmic extract, prepared from embryonic lens cells, in a reaction mixture which contained S-adenosyl-methionine as methyl group donor. The appearance of m7 GpppGm was a function of time and lens extract concentration. S-adenosyl-homocysteine inhibited both the m7G and Gm modification reactions. Analogues of GpppG, pG, ppG and pppG were relatively ineffective as substrates.
D000818:Animals; D002642:Chick Embryo; D007908:Lens, Crystalline / Q000201:enzymology*; D008780:Methyltransferases / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger
D012333:RNA, Messenger; D008780:Methyltransferases