Production of [14C]patulin by Penicillium patulum.
Factors affecting the production of [14C]patulin from [1-14C]acetate by replacement cultures of Penicillium patulum have been investigated. Incorporation of [1-14C]acetate into patulin reached a maximum with 6- to 8-day-old cultures incubated at 28 degrees C for 8 h in a replacement medium containing 0.1 M glucose, inorganic salts, and undiluted [1-14C]acetate. The specific activity of [14C]patulin obtained from this method was 34 mCi/mmol when 0.5 mCi of [1-14C]acetate was supplied to the replacement medium.
D000085:Acetates / Q000378:metabolism; D002250:Carbon Radioisotopes; D005947:Glucose / Q000378:metabolism; D010365:Patulin / Q000096:biosynthesis*; D010407:Penicillium / Q000378:metabolism*; D011714:Pyrans / Q000096:biosynthesis*
D000085:Acetates; D002250:Carbon Radioisotopes; D011714:Pyrans; D010365:Patulin; D005947:Glucose