Determination of protein molecular weights in denaturing solvents using glyceryl-CPG.
Glyceryl-CPG is controlled-pore glass whose surface has been chemically modified to block its slight negative charge in aqueous solution. In contrast to normal controlled-pore glass, glyceryl-CPG can be used successfully for gel filtration of proteins in a variety of denaturing solvents, viz., 8 M urea, 6 M guanidine hydrochloride, and 0.1% sodium dodecyl sulphate.  It has been found that glass with a mean pore diameter near 35 nm is suitable for the molecular weight range 17,000-100,000. Glyceryl-CPG with a smaller pore diameter is more satisfactory for molecular weights below 30,000.  The stable pore size and bed dimensions of controlled-pore glass allows comparison of the conformation of a particular protein in different solvents to be made with the same column.
D002850:Chromatography, Gel; D005898:Glass; D005990:Glycerol; D007700:Kinetics; D008722:Methods; D008970:Molecular Weight; D011489:Protein Denaturation; D011506:Proteins; D012997:Solvents
D011506:Proteins; D012997:Solvents; D005990:Glycerol