Differential partitioning of tRNAs between micellar and aqueous phases: a convenient gel filtration method for separation of tRNAs.
Partition coefficients (Kp) of phenylalanine, glutamic acid-II and tyrosine tRNAs between water and micellar cetyltrimethylammonium chloride (CTACl) have been determined to be 5600, 520 and 3100, respectively, by gel filtration on Sephadex G-100-120. Equations for obtaining Kp values from quantitative gel filtration have also been derived. The use of this method for the separation of tRNAs is demonstrated.
D002593:Cetrimonium Compounds; D002850:Chromatography, Gel / Q000379:methods; D005971:Glutamates; D005998:Glycine; D008433:Mathematics; D008823:Micelles; D009239:N-Formylmethionine; D010649:Phenylalanine; D000644:Quaternary Ammonium Compounds; D012343:RNA, Transfer / Q000302:isolation & purification*; D013499:Surface Properties; D014443:Tyrosine
D002593:Cetrimonium Compounds; D005971:Glutamates; D008823:Micelles; D000644:Quaternary Ammonium Compounds; D009239:N-Formylmethionine; D014443:Tyrosine; D010649:Phenylalanine; D012343:RNA, Transfer; D005998:Glycine