A quantitative assay of cortisol in human plasma by high performance liquid chromatography using a selective chemically bonded stationary phase.
The extraction and subsequent liquid chromatographic analysis of human plasma samples for cortisol is described. Extraction and chromatography are optimized, resulting in a recovery for cortisol of 96% and a detection limit of 1 microgram cortisol in 100 ml plasma. The application of two chemically modified silicas has been evaluated. The specificity of the method was tested by field desorption - mass spectrometry experiments.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008832:Microchemistry
D007202:Indicators and Reagents; D006854:Hydrocortisone