title: GLC determination of free triclocarban in blood.
abstract: A method is presented for the quantitative determination of free triclocarban in rat or human blood. The procedure involves the extraction from blood with acetone, a TLC cleanup, derivatization with N,O-bis (trimethylsily) acetonide, and GLC using an electron-capture detector. GLC-mass spectral analysis confirmed that the structure of the derivative was a bis (trimethylsilyl) molecule with one group on a nitrogen and the second group attached to the enol tautomer. The method is sensitive to 25 ng (12.5 microgram/liter of blood). Recoveries of added triclocarban in the 12.5--50-microgram/liter range weree between 80 and 90%.
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000097:blood*; D002224:Carbanilides / Q000097:blood*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D006801:Humans; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D008722:Methods; D051381:Rats
chemical_list: D000891:Anti-Infective Agents, Local; D002224:Carbanilides; C009540:triclocarban