Determination of plasma procainamide and N-acetylprocainamide concentration by high-pressure liquid chromatography.
We describe a routine method for determining concentrations of the antiarrhythmic drug procainamide and its active metabolite, N-acetylprocainamide, in plasma. A simple extraction of 1.0 ml of plasma is followed by separation and chromatographic analysis by use of a column containing microparticulate silica. p-nitro-N-(2-diethylaminoethyl)benzamide hydrochloride was synthesized and used as the internal standard. Total chromatographic time is only 7 min. The day-to-day CV during three months of daily use was less than 4% of the mean for each compound, and we saw no deterioration in column performance during this time. Phenobarbital, phenytoin, lidocaine, primidone, methsuximide, quinidine, and their metabolites do not interfere.
D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D000927:Anticonvulsants / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008722:Methods; D011342:Procainamide / Q000031:analogs & derivatives* / Q000097:blood*
D000889:Anti-Arrhythmia Agents; D000927:Anticonvulsants; D011342:Procainamide