Radioimmunological and biological measurement of prostaglandins in rabbit urine: decrease of PGE2 excretion at high NaCl intake.
The estimation of prostaglandin (PG) E2 and of PGF2alpha by radioimmunoassay is described in detail. PGE2 was measured after conversion to either PGB2 or PGF2alpha and the results compared to bioassay. The methods were used to follow the excretion of PGE2 and PGF2alpha after salt loading in rabbits. A marked reduction of PGE2 levels was observed at high NaC1 intake, while PGF2alpha excretion remained unchanged.
D000818:Animals; D001681:Biological Assay; D002845:Chromatography; D011453:Prostaglandins / Q000652:urine; D011458:Prostaglandins E / Q000652:urine*; D011460:Prostaglandins F / Q000652:urine*; D011817:Rabbits; D011863:Radioimmunoassay; D012965:Sodium Chloride / Q000494:pharmacology*
D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D012965:Sodium Chloride