Binding of 16alpha-gitoxin and its 16-acetate to human serum albumin.
The binding of semi-synthetic (16alpha-gitoxin and its 16-acetate) and naturally occurring cardioactive glycosides (digitoxin, ouabain) to human serum albumin was studied using equilibrium dialysis, ultracentrifugation and gel filtration. 16alpha-gitoxin was 75% bound and its 16-acetate 95%. The percentage binding of digitoxin and ouabain was in good agreement with values reported in literature.
D002850:Chromatography, Gel; D003956:Dialysis; D004071:Digitalis Glycosides / Q000097:blood*; D004074:Digitoxin / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D010042:Ouabain / Q000097:blood; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D014461:Ultracentrifugation
D004071:Digitalis Glycosides; D012709:Serum Albumin; D010042:Ouabain; D004074:Digitoxin