title: The determination of tryptophan and tyrosine in native proteins on the basis of inner filter effect in spectrofluorimetry.
abstract: Determination of tryptophan content in proteins, or tyrosine in proteins not containing tryptophan, is described. The method was applied to five proteins containing from 2 to 8 tryptophan residues per molecule. It was found that the simultaneous presence of a large amount of tyrosine in the protein has no influence on the accuracy of tryptophan determination.
mesh_terms: D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014364:Tryptophan / Q000032:analysis*; D014443:Tyrosine / Q000032:analysis*
chemical_list: D011506:Proteins; D014443:Tyrosine; D014364:Tryptophan