title: [Regulation of the IgM immune response. I. Changes in the affinity of carp anti-DNP-antibodies during immunization depending on the antigen dose].
abstract: The affinities of anti-DNP-antibodies (IgM) of carp, immunized with different doses of dinitrophenylated human serum albumin in complete Freund's adjuvant, were determined by a modified Farr-technique using the hapten epsilon-DNP-L-Lysine. Very low affinity antibodies were formed in the early immune response (Ko = 0.2 - 1.0-10(5) M-1). A progressive increase in IgM antibody affinity occurred during the immune reaction. After immunization with low doses of antigen (0.01 and 0.1 mg) the affinity increased up to four weeks (Ko = 3.2 and 9.1-10(5) M-1) and decreased in the subsequent three weeks (K = 0.8 and 1.2-10(5) M-1). Following immunization with a higher dose of antigen (1 and 10 mg), the affinity increased up to seven weeks (Ko = 4.9 and 11.9-10(5) M-1). The highest antibody concentration in all groups were observed at the maximum of affinity.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002347:Carps / Q000276:immunology*; D003530:Cyprinidae / Q000276:immunology*; D004136:Dinitrobenzenes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005620:Freund's Adjuvant; D006241:Haptens; D007114:Immunization; D007075:Immunoglobulin M / Q000096:biosynthesis; D009578:Nitrobenzenes / Q000276:immunology*; D013997:Time Factors
chemical_list: D004136:Dinitrobenzenes; D006241:Haptens; D007075:Immunoglobulin M; D009578:Nitrobenzenes; D005620:Freund's Adjuvant
vernacular_title: Untersuchungen zur Regulation der IgM-Immunantwort. I. Veränderungen der Affinität von Karpfen-anti-DNP-Antikörpern im Verlaufe der Immunisierung in Abhängigkeit von der Antigendosis