4-(p-Nitrobenzyl)pyridine as a spray reagent for the thin-layer chromatographic detection of dimethyl tetrachloroterphthalate herbicide.
The developed thin-layer chromatogram of DCPA (Dacthal) is sprayed with 4-(p-nitrobenzyl)pyridine chromogenic reagent followed by ammonium carbonate solution. The chromatogram is then heated in an over at 130 degrees C for 20 minutes. When cool, the chromatogram is sprayed with tetraethylenepentamine reagent. DCPA spots appear blue against a white background. The sensitivity of this test is about 1 microgram. The procedure is useful to detect DCPA in water and in soil.
D002855:Chromatography, Thin Layer; D005618:Fresh Water / Q000032:analysis; D006540:Herbicides / Q000032:analysis*; D007202:Indicators and Reagents; D009578:Nitrobenzenes; D010573:Pesticide Residues / Q000032:analysis; D010795:Phthalic Acids / Q000032:analysis*; D011725:Pyridines; D012987:Soil / Q000032:analysis
D006540:Herbicides; D007202:Indicators and Reagents; D009578:Nitrobenzenes; D010573:Pesticide Residues; D010795:Phthalic Acids; D011725:Pyridines; D012987:Soil; C008633:4-(4-nitrobenzyl)pyridine; C007220:dimethyl 2,3,5,6-tetrachloroterephthalate