Evidence for 16beta-hydroxylation of estrogens by guinea pig liver slices.
In an earlier communication, we conclusively proved that estrone-3-sulfate could be hydroxylated in the 16alpha position by guinea pig liver slices. Disulfates of 16alpha-hydroxyestrone and estriol were identified. Suggestive evidence for 6-hydroxylated metabolites was also mentioned. A careful reinvestigation has now shown that the steroid disulfate fraction is composed of 16alpha- and 16beta-hydroxylated steroids. 16beta-Hydroxyestrone in particular is an important quantitative metabolite of estradiol-17 beta. It is concluded that no firm evidence is available for the formation of 6-hydroxysteroids in the tissue system under consideration.
D000818:Animals; D003460:Crystallization; D004967:Estrogens / Q000378:metabolism*; D004970:Estrone / Q000378:metabolism; D006168:Guinea Pigs; D006914:Hydroxysteroids / Q000302:isolation & purification; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism
D004967:Estrogens; D006914:Hydroxysteroids; D004970:Estrone; D013250:Steroid Hydroxylases