[Determination of cobalt in small samples of tissues and organs of the rainbow trout (Salmo gairdneri) by flameless atomic-absorption-spectroscopy (author's transl)].
In order to increase our knowledge about the distribution pattern of the trace mineral cobalt in fish, cobalt contents of tissues and inner organs of rainbow trout were analysed. Cobalt determinations were performed by flameless atomic-absorption-spectroscopy with a heated graphite analyzer after digestion of the organic material with concentrated nitric acid in a closed system under pressure (pressure decomposition). In order to concentrate and separate the trace cobalt quantities from the main matrix constituents a micro-solvent-extraction system with ammonium pyrrolidinedithiocarbamate (APDC) as heavy metal chelating reagent and toluene as extraction solvent was developed. The extraction procedure yielded a recovery of more than 95% as determined the use of an isotope method (radiotracer experiments with Co-57). Results (calculated on wet weight basis) showed that the element under study was mainly accumulated in the kidney (0.195-0.449 microgram/g). Smaller amounts were found in blood (0.038-0.090 microgram/g), spleen (0.015-0.078 microgram/g, and liver 0.015-0.068 microgram/g). The values for skeletal and muscle tissue were low and ranged from 0.007 to 0.014 microgram/g a 0.002 to 0.007 microgram/g respectively.
D000818:Animals; D003035:Cobalt / Q000032:analysis*; D008832:Microchemistry; D014018:Tissue Distribution; D014337:Trout
D003035:Cobalt
Bestimmung von Kobalt in kleinen Gewebe- und Organproben der Regenbogenforelle (Salmo gairdneri) durch flammenlose Atom-Absorptions-Spektroskopie.