Rapid and sensitive, colorimetric determination of the anomers of D-glucose with D-glucose oxidase, peroxidase, and mutarotase.
A modification, utilising mutarotase, of an enzymic, colorimetric system for determining D-glucose with D-glucose oxidase, peroxidase, and ABTS was satisfactory for the assay of the anomers of D-glucose in aqueous solution. The time required for a single assay is approximately 10 min, and the lower limit is 0.4 microgram of D-glucose. The method is applicable to the anomer analysis of D-glucose released by enzymic hydrolysis of D-glucosides.
D002238:Carbohydrate Epimerases; D003124:Colorimetry / Q000379:methods; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D005959:Glucosidases; D006735:Horseradish Peroxidase; D010544:Peroxidases
D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D010544:Peroxidases; D005959:Glucosidases; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase; D005947:Glucose