The influence of alpha thioglycerol on erythropoiesis in fetal mouse liver cell cultures.
alpha-Thioglycerol has been shown to increase hemoglobin synthesis in suspension cultures of fetal mouse liver cells. The mechanism of this effect is unknown but appears to be directly on S-phase cells increasing their sensitivity to erythropoietin. alpha-Thioglycerol inhibits heme synthesis when S-phase cells have been removed by prior treatment with hydroxyurea. The stimulatory or inhibitory effects of thiols depending on the proportion of cells in S-phase suggests that extreme caution should be used when interpreting cell kinetic studies of cultured cells if thiols are present in the cultures.
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin; D005990:Glycerol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006418:Heme / Q000096:biosynthesis; D006918:Hydroxyurea / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology; D051379:Mice; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D013438:Sulfhydryl Compounds; D004921:Erythropoietin; D006418:Heme; D004247:DNA; D005990:Glycerol; D006918:Hydroxyurea