title: The effect of dietary protein deprivation on protein synthesis in the isolated liver parenchymal cell.
abstract: 1. Parenchymal cells were isolated from the liver of rats that had been deprived of dietary protein for 3 weeks. 2. The cells were two-thirds the diameter of those derived from livers of normal animals and consumed oxygen at a rate of 16-2 +/- 2-7 mu1/h per 10(6) viable cells, half the normal value. 3. Albumin and transferrin were synthesized at rates of 0-94 +/- 0-12 and 0-60 +/- 0-07 microgram/h per 10(6) viable cells respectively and urea at a rate of 0-77 +/- 0-12 microgram/h per 10(6) cells. This represents a 25-50% decrease in the rates of synthesis measured in cells isolated from normal livers. 4. The results are discussed in relation to the long-term effects of malnutrition on liver cell function.
mesh_terms: D000418:Albumins / Q000096:biosynthesis; D000818:Animals; D002478:Cells, Cultured; D004044:Dietary Proteins / Q000008:administration & dosage; D008099:Liver / Q000378:metabolism*; D008297:Male; D010101:Oxygen Consumption; D014176:Protein Biosynthesis; D011488:Protein Deficiency / Q000378:metabolism*; D051381:Rats; D014168:Transferrin / Q000096:biosynthesis; D014508:Urea / Q000096:biosynthesis
chemical_list: D000418:Albumins; D004044:Dietary Proteins; D014168:Transferrin; D014508:Urea