[Quantitative assessment of catecholamine fluorescence detected by the Falck method].
While using Falck's method, a number of simple and handy additional ways were suggested to carry on quantitative estimation of the fluorescence change of noradrenergic and dopaminergic terminals in a number of nuclei of the central nervous system. A device was used, which allows to obtain samples of brain of a given size and in a definite plane. The method we used makes it possible to treat simultaneously a great number of material at all the stages of work. Fluorescence intensity was measured by means of fluorometric device with the stop of a large diameter. Tests with the substances lowering the content of catecholamines showed that at the content of catecholamine less than 60% one can observe linear dependance between fluorescence intensity and the content of catecholamine in tissues. In the sphere of high concentrations such linearity does not exist.
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002395:Catecholamines / Q000032:analysis*; D051381:Rats; D012110:Reserpine / Q000494:pharmacology; D013050:Spectrometry, Fluorescence
D002395:Catecholamines; D012110:Reserpine
Kolichestvennaia otsenka fliuorestsentsii katekholaminov, vyiavliaemykh metodom Fal'ka.