Radioimmunoassay of atropine in plasma.
Antibodies, against atropine, were produced in rabbits immunized with atropine conjugated to bovine serum albumin. The antisera possessed a high binding affinity and were quite specific. The sensitivity of the method allowed detection of 6.25 ng of atropine per ml of plasma in a 10-microliter specimen. The method does not require an extraction procedure and can be performed using very small volumes  of plasma. Plasma concentration-time profiles were determined by this method in dogs after rapid i.v. administration of atropine. Atropine declined from plasma in a biexponential fashion, exhibiting a terminal half-life of approximately 2.1 hours.
D000818:Animals; D000918:Antibody Specificity; D001285:Atropine / Q000097:blood* / Q000276:immunology; D004285:Dogs; D006241:Haptens; D007700:Kinetics; D008297:Male; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine
D006241:Haptens; D012710:Serum Albumin, Bovine; D001285:Atropine