Glucosinolates and derived products in cruciferous vegetables: gas-liquid chromatographic determination of the aglucon derivatives from cabbage.
A procedure is described for identifying and quantitatively determining the glucosinolates found in edible cabbage. The intact glucosinolates are extracted and their aglucons are released into solvent after a special enzymatic hydrolysis step. The resulting isothiocyanates and oxazolidinethiones are separated by a gas-liquid chromatographic procedure that allows the individual aglucons to be identified and their amounts to be estimated by comparison with an internal reference.
D002849:Chromatography, Gas; D005960:Glucosides / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D008722:Methods; D014118:Toxins, Biological / Q000032:analysis*; D014675:Vegetables / Q000032:analysis*
D005960:Glucosides; D006027:Glycosides; D014118:Toxins, Biological