High-performance thin-layer chromatographic determination of psychopharmacologic agents in blood serum.
High-performance thin-layer chromatography affords a rapid, sensitive method for determination of psychopharmacologic agents in blood serum samples. Quantitation of the representative drugs chlorpromazine, amitriptyline, nortriptyline, imipramine, and desipramine at levels as low as 5 ng/ml is demonstrated by scanning the developed thin-layer plates with a chromatographic spectrophotometer in the ultraviolet absorption mode. Neither derivatization prior to, nor color development after chromatographic separation is required to achieve sensitivity and reproducibility of determinations.
D000639:Amitriptyline / Q000097:blood; D002746:Chlorpromazine / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003891:Desipramine / Q000097:blood; D006801:Humans; D007099:Imipramine / Q000097:blood; D008722:Methods; D009661:Nortriptyline / Q000097:blood; D011619:Psychotropic Drugs / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D011619:Psychotropic Drugs; D000639:Amitriptyline; D009661:Nortriptyline; D007099:Imipramine; D003891:Desipramine; D002746:Chlorpromazine