Fractionation of Saprolegnia diclina (Oomycetes) satelite DNAs by AgNO3/Cs2SO4 density gradient centrifugation.
Saprolegnia diclina DNA has been fractionated using preparative AgNO3/Cs2SO4 and CsCl density gradients. In addition to the previously identified major satellite DNA, there are two minor DNA components banding at 1.682 and 1.701 g - cm(-3) in CsCl. Purified major satellite DNA bands at 1.707 g - cm(-3) giving a base composition of 48% G + C in good agreement with 47% G + C calculated from its Tm value. The nuclear DNA base composition is 58% G + C by both methods. The base composition of the major satellite DNA suggests that it may represent ribosomal DNA cistrons.
D002499:Centrifugation, Density Gradient / Q000379:methods; D004247:DNA / Q000302:isolation & purification; D004276:DNA, Satellite / Q000302:isolation & purification; D005658:Fungi / Q000032:analysis*; D007202:Indicators and Reagents; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009868:Oomycetes / Q000032:analysis*; D013696:Temperature
D004276:DNA, Satellite; D007202:Indicators and Reagents; D004247:DNA