Simultaneous analysis of bases, nucleosides and nucleoside mono- and polyphosphates by high-performance liquid chromatography.
A single-column high-performance liquid chromatographic system for the simultaneous separation of bases, nucleosides and nucleoside mono- and polyphosphates has been developed, in which a strong porous anion-exchange resin (Aminex A-14) is used. The chromatographic run, carried out at 55 degrees and at alkaline pH by using a linear gradient both of ionic strength and pH, takes less than 225 min. The quantitative application of the described procedure to the analysis of cell nucleotide pools is reported.
D000818:Animals; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008099:Liver / Q000032:analysis; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D011687:Purines / Q000032:analysis*; D011743:Pyrimidines / Q000032:analysis*; D051381:Rats; D015003:Yeasts / Q000032:analysis
D002021:Buffers; D009705:Nucleosides; D009711:Nucleotides; D011687:Purines; D011743:Pyrimidines