title: Infectivity reacquisition by Trypanosoma brucei brucei cultivated with tsetse salivary glands.
abstract: Reacquisition of infectivity four mice was observed in cultures of Trypanosoma brucei brucei grown in the presence of tests fly salivary gland explants in a medium based on the amino acid composition of Glossina hemolymph and containing fetal bovine serum. High infection rates were obtained in mice inoculated with about 1.5 X 10(8) organisms. Infectivity reacquisition was correlated with invasion of the salivary glands by the parasites. Few small trypanosomes with subterminal kinetoplasts (metacyclic-like forms) were found in the infective inoculums. The parasitemias in mice consisted of pleomorphic cultivable trypanosomes. Cultures initiated by these organisms and then placed with the head-salivary gland preparations became infective for mice.
mesh_terms: D000818:Animals; D051379:Mice; D009924:Organ Culture Techniques; D012469:Salivary Glands / Q000469:parasitology*; D013997:Time Factors; D014346:Trypanosoma brucei brucei / Q000254:growth & development / Q000502:physiology*; D014352:Trypanosomiasis / Q000469:parasitology*; D014370:Tsetse Flies / Q000469:parasitology