Methods for the rapid separation and estimation of the major lipids of arteries and other tissues by thin-layer chromatography on small plates followed by microchemical assays.
Methods are described for the rapid separation of the major individual phospholipids and neutral lipids of tissues by thin-layer chromatography on small glass plates (75 X 75 mm), and for the specific microchemical estimation of separated lipids and for determination of fatty acid composition and radioactivity. The overall method, involving tissues extraction, thin-layer chromatographic separation and assay has been evaluated using pure standards and biological samples and gives good reproducibility and almost complete recovery of lipids.
D001158:Arteries / Q000032:analysis*; D002784:Cholesterol / Q000032:analysis / Q000097:blood; D002788:Cholesterol Esters / Q000032:analysis; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer / Q000379:methods; D005227:Fatty Acids / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005453:Fluorescence; D005470:Fluorometry; D005989:Glycerides / Q000032:analysis; D006358:Hot Temperature; D006801:Humans; D007455:Iodine; D008055:Lipids / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D008832:Microchemistry; D010743:Phospholipids / Q000302:isolation & purification; D010758:Phosphorus / Q000032:analysis
D002788:Cholesterol Esters; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D008055:Lipids; D010743:Phospholipids; D010758:Phosphorus; D007455:Iodine; D002784:Cholesterol