High-pressure liquid chromatographic determination of tetracyclines in urine.
The quantitation of oxytetracycline, tetracycline, and chlortetracycline was accomplished by high-pressure liquid chromatography using an anion-exchange column. The tetracyclines were extracted from urine as their calcium complexes. Concentrations as low as 12 microgram of oxytetracycline/ml and 4 microgram of tetracycline and chlortetracycline/ml were quantitated accurately. The relative standard deviation of the method varied from 0 to 5%.
D000818:Animals; D002417:Cattle; D002751:Chlortetracycline / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D008722:Methods; D010118:Oxytetracycline / Q000652:urine; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D013752:Tetracycline / Q000652:urine; D013754:Tetracyclines / Q000652:urine*
D013754:Tetracyclines; D013752:Tetracycline; D002751:Chlortetracycline; D010118:Oxytetracycline