Cytotoxins against a granulocyte antigen system: detection by a new method employing cytochalasin-B-treated cells.
A method for demonstrating granulocyte cytotoxins using a modified microdroplet dye exclusion technique and cytochalasin-B-treated cells is described. Cytochalasin-B-treated granulocytes were reacted against 223 sera from multitransfused pregnant and renal transplant patients. Incidence of granulocyte cytotoxins was 11.2%. Analysis (2 X 2 contingency tables) of the reactivity of GCT+ sera showed highly significant (p less than 0.001) positive associations among 5 sera and a significant (p less than 0.001) negative association with another serum. The data are consistent with the hypothesis that a granulocyte antigen system is detected by cytotoxins and that granulocyte antigens 'Grl' and 'Gr2' may be products of allelic genes.
D000483:Alleles; D000941:Antigens / Q000302:isolation & purification; D003571:Cytochalasin B; D003601:Cytotoxicity Tests, Immunologic; D005796:Genes; D006098:Granulocytes / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology
D000941:Antigens; D003571:Cytochalasin B