Equivalency of linear least squares curve fitting and reciprocal functions in protein circular dichroic spectra analysis.
Two methods for the analysis of the circular dichroism spectra of proteins for determination of secondary structure have been examined. These are the linear curve fitting of the data, minimized in the least squares sense, and the method of reciprocal functions proposed by C.C. Baker and I. Isenberg, Biochemistry 15 (1976) 629. It is shown that the use of these two methods give results that are identical, providing the same set of reference spectra are used in each case, and, therefore, that no new information is obtained by the use of either one over the other.
D001426:Bacterial Proteins; D002942:Circular Dichroism; D008433:Mathematics; D011506:Proteins; D012269:Ribosomal Proteins; D014335:Tropomyosin
D001426:Bacterial Proteins; D011506:Proteins; D012269:Ribosomal Proteins; D014335:Tropomyosin