Immune responses of outbred guinea pigs to the sequential polymer poly(L-Tyr-L-Glu-L-Ala-Gly): association of response to strain 13 histocompatibility locus.
Outbred Hartley guinea pigs were immunized with the sequential polymer poly(L-Tyr-L-Glu-L-Ala-Gly) (T-G-A-Gly)n, the random copolymer poly(Glu50,Tyr50) (GT), and a low dose of dinitrophenylated guinea pig albumin (DNP-GPA). Only the guinea pigs that carry strain 13 histocompatibility antigen responded to these three antigens as measured by the antigen-binding assay and delayed skin reaction. The immune response (Ir) genes controlling responses to (T-G-A-Gly)n, GT, or DNP-GPA, were found to be distinctive genes. Nonresponder inbred strain 2 guinea pigs were able to produce anti(T-G-A-Gly)n antibodies when immunized with (T-G-A-Gly)n and methylated bovine serum albumin (MBSA) complex, although there was no delayed skin reaction and in vitro cultured lymphocytes did not respond. The in vitro response to (T-G-A-Gly)n of cultured lymphocytes from responder animals was inhibited by strain 2 antistrain 13 alloantisera but not the strain 13 anti-strain 2 alloantisera.
D000409:Alanine / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D004136:Dinitrobenzenes / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D005796:Genes; D005971:Glutamates / Q000276:immunology; D005998:Glycine / Q000276:immunology; D006168:Guinea Pigs; D006648:Histocompatibility; D007106:Immune Sera / Q000494:pharmacology; D007518:Isoantibodies; D010455:Peptides / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology; D012882:Skin Tests; D014443:Tyrosine / Q000276:immunology
D004136:Dinitrobenzenes; D005971:Glutamates; D007106:Immune Sera; D007518:Isoantibodies; D010455:Peptides; D012710:Serum Albumin, Bovine; D014443:Tyrosine; D000409:Alanine; D005998:Glycine