Determination of urinary total phenolic compounds with use of 4-aminoantipyrine: suggested screening test for hyperthyroidism and for catecholamine-producing tumor.
We describe a method for determining those urinary total phenolic compounds that are tyrosine analogs or metabolites, such as thyroxine and catecholamines. The urine sample, 4-aminoantipyrine in carbonate-bicarbonate buffer, and potassium ferricyanide solution are mixed and the quinoneimine dye that forms is measured at 500 nm. Some cases of hyperthyroidism, diabetes mellitus, nephrosis, obesity, hypertension, or catecholamine-producing tumor showed above-normal values, so that this determination seems useful as a screening test for these disorders.
D002395:Catecholamines / Q000378:metabolism; D003937:Diagnosis, Differential; D006801:Humans; D006980:Hyperthyroidism / Q000175:diagnosis* / Q000652:urine; D007202:Indicators and Reagents; D008403:Mass Screening; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000652:urine; D010636:Phenols / Q000652:urine*; D013053:Spectrophotometry / Q000379:methods
D002395:Catecholamines; D007202:Indicators and Reagents; D010636:Phenols