Microdetermination of dolichol in tissues.
A method is described for the rapid purification and analysis of tissue dolichol on a nanomole scale. The assay is based on a radioisotope dilution technique in which [3H]dolichyl palmitate is used as tracer and acetylation with [14C]acetic anhydride serves as the quantitating reaction. The acetylation conditions were characterized with respect to time, temperature and concentration of reagents. When this method was applied to pig liver, a value was obtained which fell within the range of previously published results based on a gravimetric assay. In chicken, dolichol levels were found to be high in oviduct and low or absent in red blood cells and plasma.
D000818:Animals; D002250:Carbon Radioisotopes; D002645:Chickens; D002850:Chromatography, Gel; D004224:Diterpenes / Q000032:analysis*; D004286:Dolichols / Q000032:analysis* / Q000097:blood; D004912:Erythrocytes / Q000032:analysis; D005260:Female; D007553:Isotope Labeling / Q000379:methods; D008099:Liver / Q000032:analysis; D008832:Microchemistry; D009928:Organ Specificity; D010057:Oviducts / Q000032:analysis; D013552:Swine; D014316:Tritium
D002250:Carbon Radioisotopes; D004224:Diterpenes; D004286:Dolichols; D014316:Tritium