title: [Chemical and immunogenic properties of the capsular antigen of Staphylococcus aureus. I. purification and chemical characteristics of the capsular antigen].
abstract: Crude capsular material isolated from S. aureus strain 1193/74 was characterized chemically and subjected to purification by 4 different methods. The best results were obtained by the method including deproteinization of the material with a mixture of chloroform and butanol, fractional precipitation with ethanol and separation on SE-Sephadex. Galactose, uronic acids, and a small amount of protein served as components characteristic of the purified capsular material.  Galacturonic and glucuronic acids were identified amoung the uronic acids. Besides, there were revealed other acids with a carbohydrate base, whose nature is still obscure.
mesh_terms: D000942:Antigens, Bacterial / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004586:Electrophoresis; D008722:Methods; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial
vernacular_title: Izuchenie khimicheskikh i immunogennykh svoĭstv kapsul'nogo antigena Staphylococcus aureus. Soobshchenie I. Ochistka i khimicheskaia kharakteristika kapsul'nogo antigena.