Freezing technique for microtoming long specimens of small width and/or depth.
A technique is described for sectioning long but thin and/or narrow biological specimens using a freezing stage and sliding microtome. A water-saturated wood veneer platform is frozen to the stage and a saturated specimen is frozen to the veneer. The technique, which is inexpensive, permits cutting sections or viewing surfaces from specimens up to 8 cm long, while protecting the microtome knife from damage.
D005615:Freezing; D005629:Frozen Sections; D008867:Microtomy / Q000379:methods*