A semi-automated measurement of urinary catecholamines using high-speed ion-exchange column chromatography.
Semi-automated measurement of urinary catecholamines, specific for dopa, noradrenaline, adrenaline and dopamine and other metabolites has been developed. Steps included alumina extraction, separation by high-speed ion-exchange column chromatography and fluorescent development by trihydroxyindole (THI) method without interference by other fluorescent substances. Recovery, reproducibility and sensitivity were satisfactory. alpha-Methyldopa, a metabolite of alpha-methyldopa, and isoproterenol were separated completely without interfering with the measurement of noradrenaline, adrenaline and dopamine.
D000328:Adult; D001322:Autoanalysis; D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000652:urine; D004837:Epinephrine / Q000652:urine; D006801:Humans; D009638:Norepinephrine / Q000652:urine
D002395:Catecholamines; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine