Determination of the rodenticide difenacoum in biological materials by high-pressure liquid chromatography with confirmation of identity by mass spectrometry.
A method for determining difenacoum in liver, plasma, urine and feedingstuffs by high-pressure liquid chromatography is described. Samples are cleaned up by molecular exclusion chromatography on porous glass. In some cases this also serves for determination; if not, the separated difenacoum is determined on an adsorption column. Identity is confirmed by chemical ionisation mass spectrometry. Recoveries at levels of 0.025-5 ppm from plasma were 101-113% by exclusion chromatography alone and 93-101% after adsorption chromatography. Recoveries from liver after both chromatographic steps were 62-86%. Reasons for the lower recoveries from liver are suggested.
D015110:4-Hydroxycoumarins / Q000032:analysis* / Q000097:blood / Q000652:urine; D000327:Adsorption; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000032:analysis; D013058:Mass Spectrometry; D012378:Rodenticides / Q000032:analysis* / Q000097:blood / Q000652:urine; D014020:Tissue Extracts / Q000032:analysis
D015110:4-Hydroxycoumarins; D012378:Rodenticides; D014020:Tissue Extracts