Some observations on gonadotropin inhibitor (anti-LH) for human urine--with discussion on its possible source of origin.
Physicochemical characteristics of an anti-LH substance, partially purified from human urine, has been determined. It is an acid-precipitable, sialic acid containing glycoprotein with a molecular weight of 162,000. This substance on mg to mg basis has a 50 fold biological potency when compared to that of the crude gonadotropin inhibiting material. Dialysed human pineal protein extracts also showed anti-LH activity which was not attributed to sialidase activity. Polyacrylamide gel electrophoresis of such pineal extracts revealed a glycoprotein component with the same mobility as that of the urinary anti-LH protein. These observations suggest a possibility that the human pineal gland may be associated either with secretion or storage of this gonadotropin inhibiting entity.
D000818:Animals; D001681:Biological Assay; D005260:Female; D006023:Glycoproteins / Q000494:pharmacology / Q000652:urine; D006727:Hormone Antagonists / Q000652:urine*; D006801:Humans; D007986:Luteinizing Hormone / Q000037:antagonists & inhibitors*; D008297:Male; D051379:Mice; D008970:Molecular Weight; D010870:Pineal Gland / Q000187:drug effects; D012794:Sialic Acids / Q000032:analysis; D014599:Uterus / Q000187:drug effects
D006023:Glycoproteins; D006727:Hormone Antagonists; D012794:Sialic Acids; D007986:Luteinizing Hormone