Pyridoxal 5'-phosphate as an activator of the apoenzyme of alanine aminotransferase in human serum.
The relationship between reaction conditions and the stimulation of alanine aminotransferase by pyridoxal 5'-phosphate was investigated. Reaction conditions given for optimized alanine aminotransferase measurements are also optimal for measurements in the presence of pyridoxal 5'-phosphate taking into consideration the inhibitory effect of phosphate buffer. Addition of 150 mumol/1 pyridoxal 5'-phosphate to the reaction mixture guarantees a maximal stimulation after a pre-incubation period of 10 min. Factors influencing the magnitude of stimulation rates of both aminotransferases in different patient groups by pyridoxal 5'-phosphate are discussed.
D000410:Alanine Transaminase / Q000097:blood* / Q000378:metabolism; D001051:Apoenzymes / Q000097:blood* / Q000378:metabolism; D001059:Apoproteins / Q000097:blood*; D004789:Enzyme Activation; D006801:Humans; D011732:Pyridoxal Phosphate / Q000378:metabolism*; D013997:Time Factors
D001051:Apoenzymes; D001059:Apoproteins; D011732:Pyridoxal Phosphate; D000410:Alanine Transaminase