[Gel-permeating chromatography of interacting multi-component systems. I. Study of protein isomerization by the method of gel-permeation chromatography. II. Method of determining the kinetic constant of isomerization].
A technique is suggested of determining the isomerization constants by means of gel-permeation chromatography. Chromatograms of proteins and expressions for statistical moments of their distribution along the chromatographic column obtained in the authors previous work [1], are used. A necessary condition for optimal setup of the GPCh experiments is found. Fulfilment of this condition provides a maximal accuracy of determination of the isomerization constants.
D002850:Chromatography, Gel; D007536:Isomerism; D011506:Proteins
D011506:Proteins
Gel'-pronikaiushchaia khromatografiia vzaimodeistvuiushchikh mnogokomponentnykh sistem. I. Izuchenie izomerizatsii belkov metodom gel'pronikaiushcheĭ khromatografii. 2. Metodika opredeleniia kineticheskikh konstant izomerizatsii.