A simple, rapid preparation of alpha[32P]-labelled adenosine diphosphate.
Hexokinase (EC 2.7.1.1) will convert commercially available alpha-[(32)P]-labelled ATP into alpha-[(32)P]-labelled ADP. A simple, rapid isolation procedure for the alpha-[(32)P]-labelled ADP is described and this synthetic method can be used for the preparation of other alpha-[(32)P]-labelled nucleoside diphosphates.
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006593:Hexokinase; D007553:Isotope Labeling / Q000379:methods; D010761:Phosphorus Radioisotopes
D010761:Phosphorus Radioisotopes; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006593:Hexokinase