Isolation, extraction, and measurement of acetylcholine from Lactobacillus plantarum.
The isolation, extraction, and spectrophotometric determination of acetylcholine from Lactobacillus plantarum ATCC 10241 is described. Acetylcholine was extracted with a mixture of sodium tetraphenylboron-butylethylketone-acetonitrile and was measured enzymatically at 340 nm.
D000097:Acetonitriles; D000109:Acetylcholine / Q000032:analysis / Q000302:isolation & purification*; D007659:Ketones; D007778:Lactobacillus / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods; D013775:Tetraphenylborate
D000097:Acetonitriles; D007659:Ketones; D013775:Tetraphenylborate; D000109:Acetylcholine