[Virus-specific poli(A)-containing RNA-transcripts in cells infected with Newcastle disease virus].
The techniques of chromatography on poly (U)-cellulose and sedimentation and electrophoresis analysis were used to study poly(A)-containing virus-specific RNA of Newcastle disease virus. The poly(A)-containing molecules were shown to be present in approximately similar amounts (60-80%) in 18S, 24S, and 35S RNA. No poly(A)-containing molecules capable of interaction with poly(U) sepharose were found in 50S RNA. In 18S RNA there are 6 individual poly(A)-containing RNA-transcripts with molecular weights well correlating with those of virus proteins. The average size of poly(A) for 18S and 24S RNA is 60-70 nucleotide residues. In the sedimentation analysis of poly; A)-containing 24S and 35S RNA eluted from poly(U)-columns, some of the material sedimented in the zone of 18S RNA. The presence of complexes of two or several molecules with the sedimentation coefficient 18S in these RNA's is suggested.
D001483:Base Sequence; D002498:Centrifugation; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009522:Newcastle disease virus; D011061:Poly A; D012367:RNA, Viral / Q000302:isolation & purification
D012367:RNA, Viral; D011061:Poly A
Virusspetsificheskie poli(A)-soderzhashchie RNK-transkripty v kletkakh, zarazhennykh virusom bolezni N'iukasl.