title: High-performance liquid chromatographic determination of perphenazine and amitriptyline hydrochloride in two-component tablet formulations.
abstract: A rapid, precise, and accurate high-performance liquid chromatographic procedure is presented for the simultaneous determination of perphenazine and amitriptyline hydrochloride in two-component tablet formulations. An aliquot of a methanolic extract of the tablet, containing trifluoperazine hydrochloride as an internal standard, is chromatographed on a nitrile bonded phase microparticulate column using a 0.005 M ammonium acetate-methanol (20:80) mobile phase. Quantitation is by peak area. The relative standard deviations for the procedure are 0.34 and 0.54% for the simultaneous determination of perphenazine and amitriptyline, respectively. Eight commercial tablet formulations were analyzed and found to contain 96.5-101.5 and 96.5-103.3% of the labeled amounts of perphenazine and amitriptyline hydrochloride, respectively.
mesh_terms: D000639:Amitriptyline / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003984:Dibenzazepines / Q000032:analysis; D003986:Dibenzocycloheptenes / Q000032:analysis; D004338:Drug Combinations; D010546:Perphenazine / Q000032:analysis*; D010640:Phenothiazines / Q000032:analysis; D013607:Tablets / Q000032:analysis
chemical_list: D003984:Dibenzazepines; D003986:Dibenzocycloheptenes; D004338:Drug Combinations; D010640:Phenothiazines; D013607:Tablets; D000639:Amitriptyline; D010546:Perphenazine