title: Effects of perinatal estrogen on mouse mammary response to corticoids in vitro.
abstract: Effects of three adrenal corticoids on in vitro mammary differentiation were compared in neonatally estrogenized (E) and uninjected control (N) BALB/c Crgl female mice. E mice were injected with 10 microgram of 17 beta-estradiol on each of the first 5 days of life. At 4 weeks of age, all mice were pretreated with 1 microgram  17beta-estradiol and 1 mg progesterone for 9 consecutive days. Groups of 10 or more entire mammary glands then were organ-cultured for 5 days at 37 degree C on chemically defined medium in 95% O2/5% CO2 with various combinations of hormones: mammotropin (M), somatotropin (S), insulin (I), thyroxine (T); and corticosterone (B), aldosterone (A), or cortisol (F). Differentiation followed a quantitative pattern of MSIT less than BMSIT less than AMSIT less than FMSIT for both E and N tissues. E tissues formed more alveoli and more lobules in vitro than N tissues with each of the corticoids tested. These findings may have pathologic significance.
mesh_terms: D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000450:Aldosterone / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects; D003345:Corticosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000187:drug effects*; D051379:Mice; D009924:Organ Culture Techniques; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D011388:Prolactin / Q000494:pharmacology; D013974:Thyroxine / Q000494:pharmacology
chemical_list: D000305:Adrenal Cortex Hormones; D007328:Insulin; D000450:Aldosterone; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D013006:Growth Hormone; D013974:Thyroxine; D003345:Corticosterone; D006854:Hydrocortisone