title: Characterization of the chondroitin sulfate produced by B16 mouse melanoma cells.
abstract: The mucopolysaccharides produced by B16 mouse melanoma cells have been isolated in milligram quantities from the spent media in which the cells were grown in the presence of 2-amino-2-deoxy-D-glucose-t and [35S]-sulfate. The mucopolysaccharides obtained by precipitation with cetylpyridinium chloride from the Pronase digest of the media were further purified by gel filtration, ion-exchange chromatography, and treatment with nucleases. The major components were identified as chondroitin-4-sulfates by identification of the hexosamine as 2-amino-2-deoxy-D-galactose, and by digestibility with hyaluronidases, chondroitinase AC, and chondro-4-sulfatase. The o.r.d. curve and i.r. spectra of these components also confirmed their similarity to chondroitin-4-sulfate from cartilage. The molecular weight of the polysaccharide chains was estimated to be in the range 90,000-120,000 by sedimentation equilibrium analysis.
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D002460:Cell Line; D002807:Chondroitin / Q000031:analogs & derivatives; D002809:Chondroitin Sulfates / Q000302:isolation & purification / Q000378:metabolism; D006595:Hexosamines / Q000032:analysis; D008545:Melanoma / Q000378:metabolism; D008970:Molecular Weight; D009905:Optical Rotatory Dispersion; D013055:Spectrophotometry, Infrared
chemical_list: D002241:Carbohydrates; D006595:Hexosamines; D002807:Chondroitin; D002809:Chondroitin Sulfates