Repeated 'superinduction' of interferon in human diploid fibroblast cultures.
Human diploid fibroblast cultures induced to make interferon by the combination of polyriboinosinic acid-polyribocytidylic adic, cycloheximide and actinomycin D degenerate thereafter, owing to the irreversible nature of the inhibition induced by actinomycin D. However, cultures superinduced with the DNA-dependent RNA synthesis inhibitor 5,6-dichloro-I-beta-D-ribofuranosylbenzimadazole (DRB) survive, owing to the reversible nature of the inhibition induced by DRB, and can again be superinduced on several occasions.
D001562:Benzimidazoles / Q000494:pharmacology; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology*; D004171:Diploidy; D005347:Fibroblasts; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology*; D007372:Interferons / Q000096:biosynthesis*; D011070:Poly I-C / Q000494:pharmacology*; D012263:Ribonucleosides / Q000494:pharmacology*; D012867:Skin
D001562:Benzimidazoles; D007369:Interferon Inducers; D012263:Ribonucleosides; D003609:Dactinomycin; D007372:Interferons; D003513:Cycloheximide; D011070:Poly I-C