[Use of a dual-frequency spectrophotometric method for determining the DNA content in the male sex cells of bulls, rams and boars].
Investigated were the absorption spectral curves of alkaline protein hydrolysates and DNA-extracts from bull, ram and boar spermatozoa employing the method of Schmidt-Tannhauser. It was established that the double-wave method could be used to determine the content of DNA in the investigated ejaculated at the following values of the two waves and coefficients: 268 nm and 280 nm; K = 1350 for ram spermatozoa, and 268 nm and 281 nm; K = 1175 for bull and boar spermatozoa. The evaluation of the quantitative content of DNA per spermatozoid of bull, ram and boar was also carried out, establishing that the figures obtained are approximately equal.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis*; D006868:Hydrolysis; D008297:Male; D011506:Proteins / Q000032:analysis; D012756:Sheep; D013054:Spectrophotometry, Atomic / Q000379:methods*; D013094:Spermatozoa / Q000032:analysis*; D013552:Swine
D011506:Proteins; D004247:DNA
Izopolzvane na dvuvulnoviia spektrofotometrichen metod za opredeliane sudurzhanieto na DNK v muzhki polovi kletki na bik, koch i nerez.