Hemoglobin interference with Du Pont Automatic Clinical Analyzer procedure for calcium.
Hemoglobin in concentrations of approximately 2.5 g/liter or greater significantly increases values for apparent calcium in serum as determined with the Du Pont aca, an interference attributable to the 575-nm absorption band of oxyhemoglobin being uncompensated by the 577--600 nm differential absorption reading of the calcium o-cresolphthalein complexone complex.
D001322:Autoanalysis / Q000379:methods; D002118:Calcium / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis; D006454:Hemoglobins; D006461:Hemolysis; D006801:Humans; D012044:Regression Analysis; D013053:Spectrophotometry / Q000379:methods
D006454:Hemoglobins; D002118:Calcium