Poly-2'-deoxy-2'-fluoro-cytidylic acid: enzymatic synthesis, spectroscopic characterization and interaction with poly-inosinic acid.
The polymerization of 2'deoxy-2'-fluoro-cytidine-diphosphate (dCflDP) by polynucleotide phosphorylase is barely detectable in the presence of Mg++ under usual experimental conditions for polymerization of nucleoside diphosphates. High concentrations of enzyme have to be used to accomplish the synthesis. Mn++ is a better activator than Mg++ for the reaction. cCflDP inhibits the polymerization of CDP and has a Km=8.8X10-3M, six times higher than CDP.- The polymer, poly (dCfl), ressembles in many respects poly(C), but not poly(dC): the acid selfstructure forms at similar pK's; interaction with poly(I) yields a 1:1 complex the CD spectrum of which is similar to that of poly(I).poly(C). Finally, the Tm's of poly(I).poly(dCfl) are comparable to those of poly(I).poly(C).
D002942:Circular Dichroism; D003597:Cytosine Nucleotides / Q000378:metabolism*; D003843:Deoxycytidine Monophosphate / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009691:Nucleic Acid Denaturation; D011066:Poly C / Q000031:analogs & derivatives / Q000096:biosynthesis*; D011069:Poly I; D011070:Poly I-C; D011089:Polydeoxyribonucleotides / Q000096:biosynthesis*; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism; D011131:Polyribonucleotides / Q000096:biosynthesis*; D013053:Spectrophotometry
D003597:Cytosine Nucleotides; D011089:Polydeoxyribonucleotides; D011131:Polyribonucleotides; D003843:Deoxycytidine Monophosphate; D011069:Poly I; D011066:Poly C; D008345:Manganese; D011117:Polyribonucleotide Nucleotidyltransferase; D008274:Magnesium; D011070:Poly I-C