Specific antibodies for desialized M and N blood group antigens.
Antisera obtained by immunizing rabbits with desialized M and N blood group glycoproteins (M' and N' glycoproteins) agglutinate desialized erythrocytes and precipitate desialized glycoproteins, but they do not react with sialic acid containing erythrocytes or glycoproteins. By absorption of anti-M' serum with N' glycoprotein, and of anti-N' serum with M' glycoprotein, reagents specifically agglutinating M' or N' erythrocytes were obtained. The absorbed anti-M' and anti-N' sera were specifically inhibited by M' or N' glycoproteins, respectively. The results show that sialic acid residues of red cell glycoproteins are not essential for the differentiation of M an N blood group determinants.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D006023:Glycoproteins / Q000494:pharmacology; D006801:Humans; D007164:Immunosorbents; D008951:MNSs Blood-Group System; D011817:Rabbits; D012794:Sialic Acids
D006023:Glycoproteins; D007164:Immunosorbents; D008951:MNSs Blood-Group System; D012794:Sialic Acids