Inhibition of cholesterol esterification in rabbit aorta by prostaglandin E2.
Cholesterol esterification in the arterial wall was investigated with cell-free preparations of intima-media from control rabbits and rabbits rendered atherosclerotic by feeding a diet containing 1% cholesterol. In the presence of 2 mM ATP and 0.1 mM CoA, the major activity for esterification of [4-14C] cholesterol added in vitro was found in the 12,000 g and 105,000 g pellets. In control animals, the activity in the latter pellet was twice that in the former. After cholesterol-feeding for 6 months, the activity increased 5-fold in the 105,000 g pellet and 2-fold in the 12,000 g pellet of the atherosclerotic intima-media. Prostaglandin E2 (PGE2) in concentrations between 2 and 12 X 10(-7) M exhibited a dose-dependent inhibition of the esterifying activity in both particulate preparations. The inhibition was 97% at PGE2 concentrations greater than 1.2 X 10(-6) M in preparations from control animals. Inhibition by PGE2 in preparations from atherosclerotic rabbits was also observed. These results suggest a possible regulatory role of PGE2 in cholesterol esterification in the arterial wall.
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001013:Aorta, Thoracic / Q000473:pathology; D001018:Aortic Diseases / Q000473:pathology; D001161:Arteriosclerosis / Q000473:pathology; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000032:analysis; D002788:Cholesterol Esters / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004036:Diet, Atherogenic; D008297:Male; D011458:Prostaglandins E / Q000494:pharmacology*; D011817:Rabbits; D013347:Subcellular Fractions / Q000032:analysis / Q000378:metabolism*
D002788:Cholesterol Esters; D011458:Prostaglandins E; D002784:Cholesterol