[Effect of cryoprotectants on protein-synthesizing activity of cell-free system from rat liver].
The protein-synthetizing activity in postmitochondrial supernatant (S15) was studied as affected by ethylene glycol, PEG-400, PEG-4000, DMSO, ethanol, methanol, and glycerol. It is found that cryoprotectants inhibit 14C-leucine incorporation in the presence of physiological concentrations of Mg2+ (5 mM) in the cell-free system. All cryoprotectants, exept of glycerol, shift the maximum of 14C-leucine incorporation towards lower Mg2+ concentrations in the cell-free system.
D000438:Alcohols / Q000494:pharmacology*; D000818:Animals; D002458:Cell Fractionation; D002474:Cell-Free System; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005026:Ethylene Glycols / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D000432:Methanol / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000378:metabolism*; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000494:pharmacology; D014176:Protein Biosynthesis; D051381:Rats
D000438:Alcohols; D003451:Cryoprotective Agents; D005026:Ethylene Glycols; D000431:Ethanol; D011092:Polyethylene Glycols; D007930:Leucine; D008274:Magnesium; D005990:Glycerol; D000432:Methanol; D004121:Dimethyl Sulfoxide
Vpliv krioprotektoriv na biloksintezuiuchu aktivnist' bezklitinnoĭ sistemi z pechinki shchuriv.