Temperature-jump experiments on thin lipid membranes in the presence of valinomycin.
Temperature jump relaxation experiments on planar lipid membranes in the presence of valinomycin were performed using the absorption of a strong light flash as an energy source for the generation of the T-jump. The relaxation of the current carried by valinomycin/Rb+ complexes was measured. The results were interpreted on the basis of a transport model which was also analyzed by voltage jump relaxation experiments. The study shows that the application of the T-jump technique provides valuable information about transport kinetics as well as the dynamics of the membrane structure. At the given experimental conditions the relaxation of the current is believed to reflect a temperature-dependent transition of the membrane to a new conformational state of low order. The relaxation could be resolved with the present technique only at low temperatures and for membranes of high microviscosity.
D001692:Biological Transport; D007700:Kinetics; D008433:Mathematics; D008567:Membranes, Artificial; D008722:Methods; D008954:Models, Biological; D010713:Phosphatidylcholines; D012413:Rubidium; D013696:Temperature; D014634:Valinomycin
D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D014634:Valinomycin; D012413:Rubidium