The effects of thromboxane B2 and 6-ketoprostaglandin F1alpha on cultured fibroblasts.
The effects of thromboxane B2 and 6-ketoprostaglandin F1alpha on the synthesis of DNA, RNA and hexosamine-containing substances were studied. Thromboxane B2 (1 microgram/ml) added to cultures in the stationary phase caused the initiation of DNA synthesis and cell proliferation in a small proportion of cells. The same amount of thromboxane B2 also increased [3H]uridine incorporation into acid-insoluble fraction by 50% in 24 h, and stimulated the production of hexosamine-containing substances 2.5 fold over the control during the first 6 h. On the other hand, 6-ketoprostaglandin F1alpha was essentially inactive on these indexes except slight stimulation of DNA synthesis.
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D007651:Keto Acids / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D013929:Thromboxane B2 / Q000494:pharmacology*; D013931:Thromboxanes / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D007651:Keto Acids; D011460:Prostaglandins F; D013931:Thromboxanes; D013929:Thromboxane B2; D012313:RNA; D004247:DNA; D005944:Glucosamine