The structure of the branching point between acidic polysaccharide and peptidoglycan in Micrococcus lysodeikticus cell wall.
An acidic polysaccharide fraction composed of glucose and N-acetylmannosaminuronic acid with a small portion of peptidoglycan was isolated by enzymic digestion and subsequent ECTEOLA-cellulose chromatography from the cell walls of Micrococcus lysodeikticus. On mild acid treatment, the fraction became Morgan-Elson positive and formed the Morgan-Elson chromogen on heating with phosphate buffer (pH 7). The product of mild acid treatment released inorganic phosphate on treatment with phosphomonoesterase. After gel-chromatography on Sephadex G-25 and DE-32, the acidic polysaccharide fraction contained less glucosamine than muramic acid. By reduction of this fraction with borohydride, a part of the glucosamine was converted into glucosaminitol. Based on these results, it is suggested that the acidic polysaccharide is linked to glucosamine by a (1-3) linkage, which is linked to the 6 position of a muramic acid residue by a phosphodiester  linkage.
D000596:Amino Acids / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D003124:Colorimetry; D005947:Glucose / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D008358:Mannose / Q000032:analysis; D008837:Micrococcus / Q000032:analysis*; D009112:Muramic Acids / Q000032:analysis; D010457:Peptidoglycan / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification; D014574:Uronic Acids / Q000032:analysis
D000596:Amino Acids; D006595:Hexosamines; D009112:Muramic Acids; D010457:Peptidoglycan; D011135:Polysaccharides, Bacterial; D014574:Uronic Acids; D005947:Glucose; D008358:Mannose