Neoplastic transformation induced by furylfuramide and nitromethylfuran of embryonic hamster cells in tissue culture.
Secondary cultures of Syrian hamster embryonic fibroblasts were tested for transformation and neoplastic properties after exposure in vitro to furylfuramine (AF-2) and other nitrofurans. Typical morphological transformation was seen in five of six cultures between 30 and 186 days following treatment with 5-10X10(6) M AF-2 for 24 h. Transformation was seen in only one of four cultures 145 days after treatment for 6 h with AF-2. Treatment with 5-10X10(6) M NMF (5-nitro-2-methylfuran) for 24 h also induced transformation after 50 and 118 days in two cultures. In contrast, untreated cultures and cultures treated with 5-10X10(-6) M NFT [4-(5-nitro-2-furyl)thiazole] for 24 h were not transformed within 200 days. Three of the six lines transformed by AF-2 and both lines transformed by NMF also became tumorigenic 7-24 days after morphologic transformation. The other three transformed lines produced nodules which regressed within a few weeks of transplantation. Untreated and treated non-transformed lines did not produce tumors during an observation period of 6 months. The tumors were classified as fibrosarcomas. The ability to form colonies in soft agar was acquired by only one tumorigenic line.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D002875:Chromosomes; D002999:Clone Cells; D006224:Cricetinae; D005347:Fibroblasts; D005354:Fibrosarcoma / Q000139:chemically induced / Q000473:pathology; D005668:Furylfuramide / Q000633:toxicity*; D008297:Male; D009368:Neoplasm Transplantation; D009581:Nitrofurans / Q000633:toxicity*
D009581:Nitrofurans; D005668:Furylfuramide