Immediate effect of nephrotoxic serum on kidney function in the dog.
Nephrotoxic anitbody rapidly fixes to glomerular basement membrane following systemic injection and immediately initiates histopathologic alterations. We studied the functional changes induced by nephrotoxic serum (NTS) in dogs starting 20 min after injection using three different protocols. By this time the GFR, UNa V and FENa had decreased in each study. Volume expansion resulted in a significantly smaller absolute increase in FENa than in the preinjection control study (1.1 vs. 2.8%, p less than 0.005), while maximum tubular secretion of PAH (TmPAH) and reabsorption of glucose (Tm glucose) stayed constant following NTS; thus TmPAH/GFR and Tm glucose/GFR increased significantly. These studies demonstrate that glomerular function is rapidly affected by NTS while absolute tubular function is relatively well maintained. Since histologic and functional changes occurred simultaneously, it seems likely that the functional changes were the direct consequence of the pathologic alterations.
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D004285:Dogs; D005919:Glomerular Filtration Rate; D005947:Glucose / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000503:physiopathology*; D007677:Kidney Function Tests; D007678:Kidney Glomerulus / Q000276:immunology; D009393:Nephritis / Q000276:immunology*; D012964:Sodium / Q000652:urine
D000906:Antibodies; D012964:Sodium; D005947:Glucose