Identification of metahydroxyamphetamine as a metabolite of amphetamine in the rat.
m-Hydroxyamphetamine (mOHA) was found as a metabolite of amphetamine in rat urine, liver perfusate and bile. The identity was established with a gas chromatograph-mass spectrometer (GC/MS) by its retention time, mass spectrum and selective-ion-monitoring of fragments representing both the side chain and the aromatic moiety. Furthermore, deuterium-labelled amphetamine was used in order to circumvent the possibility of interference by substances with similar structure of endogenous or exogenous origin. The amount of mOHA was low, about 10% of the ring hydroxylation could be accounted for as metahydroxylation.
D000661:Amphetamine / Q000378:metabolism*; D000662:Amphetamines / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D001646:Bile / Q000032:analysis; D002849:Chromatography, Gas; D006900:Hydroxylation; D008297:Male; D013058:Mass Spectrometry; D010665:Phenylpropanolamine / Q000032:analysis; D051381:Rats
D000662:Amphetamines; D010665:Phenylpropanolamine; D000661:Amphetamine