A simple, general procedure for purifying restriction endonucleases.
A simple, general method for purifying restriction endonucleases is described. The method employs precipitation of nucleic acids from crude extracts with polyethyleneimine followed by affinity chromatography on columns of heparin covalently linked to agarose. Most of the sixteen enzymes tested could be purified to a degree sufficient for DNA sequencing work by this method sometimes supplemented by at most one step of ion exchange chromatography.
D001407:Bacillus / Q000201:enzymology; D001483:Base Sequence; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004247:DNA; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D006493:Heparin; D013045:Species Specificity; D013824:Thermus / Q000201:enzymology
D006493:Heparin; D004247:DNA; D004262:DNA Restriction Enzymes