Demonstration of a collagenolytic enzyme in postmortal human colon tissue.
A collagenolytic enzyme was extracted from the 6000 g sediment of homogenized postmortal human colon tissue. The enzyme digests native collagen at neutral pH. The enzyme has been concentrated by ammonium sulfate precipitation. The collagenolytic activity was measured using [1-14C]acetylated calf skin collagen, solubilized in 0.1 g/1 acetic acid. There was a significant inactivation of the enzyme solution after the addition of mumol EDTA to the test volume.
D003094:Collagen / Q000378:metabolism*; D003106:Colon / Q000201:enzymology*; D006801:Humans; D011180:Postmortem Changes
D003094:Collagen