"Fast isoamylases" in the parotid saliva of children with cystic fibrosis and heterozygous carriers.
On electrophoresis, parotid saliva always exhibits a basic pattern of 6 isoamylases. Additional faster migrating isoamylases occur in varying numbers. These "fast isoamylases" are generated, at least in part, by deamidation. Compared with juvenile and adult controls, a significantly greater number of "fast isoamylases" was found in the parotid saliva of children with cystic fibrosis and their healthy heterozygous parents. A shift in the equilibrium between amidation and deamidation is discussed in terms of its possible connection with the metabolic defect responsible for cystic fibrosis.
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000201:enzymology*; D005260:Female; D006026:Glycoside Hydrolases / Q000032:analysis*; D006579:Heterozygote; D006801:Humans; D007517:Isoamylase / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010306:Parotid Gland / Q000378:metabolism; D012463:Saliva / Q000201:enzymology*
D006026:Glycoside Hydrolases; D007517:Isoamylase