Leucine aminopeptidase from swine kidney: purification, molecular weight, subunit and amino acid composition.
A homogeneous leucine aminopeptidase was obtained from mixed breed swine kidneys by means of chromatography on a special column. After coupling an inhibitor, N-sulfanilyl N'-butylcarbamide, to Sepharose 6B, the derivative did not absorb the enzyme, but absorbed a non-enzymatically active protein. The enzyme showed a single band on disc-gel electrophoresis. The molecular weight of the enzyme has 320,000 daltons. In 6 M guanidine solution containing 0.5% 2-mercaptoethanol at pH 8, the enzyme exhibited a molecular weight of 53,000 on equilibrium centrifugation. A similar value, 54,000, for the subunit of the enzyme was found on SDS-gel electrophoresis. The amino acid composition of the enzyme is also reported.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D007668:Kidney / Q000201:enzymology*; D007931:Leucyl Aminopeptidase / Q000302:isolation & purification*; D008722:Methods; D008970:Molecular Weight; D013552:Swine
D000596:Amino Acids; D007931:Leucyl Aminopeptidase