An enzyme-P.M.R.-spectroscopic determination of the enantiomers of galactose.
The action of D-galactose oxidase on D-galactose in the presence of oxygen afforded meso-galacto-hexodialdose quantitatively, which allowed p.m.r.-spectroscopic determination of the D enantiomer in a DL mixture. In the spectrum of the products obtained from the enzymic treatment of a mixture of D- and L-galactose, the magnitude of the aldehydrol signals derived solely from the oxidised D enantiomer, relative to those of the anomeric signals, provided the fractional content of the D enantiomer. This simple, accurate, and convenient procedure was applied to the hydrolysate of a seaweed galactan, which was also analyzed, for comparative purposes, by the fermentation technique employing D-galactose-adapted Saccharomyces cerevisiae.
D005690:Galactose; D005692:Galactose Oxidase; D007536:Isomerism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D013379:Substrate Specificity
D005692:Galactose Oxidase; D005690:Galactose