A solid-phase enzyme immunoassay for serum ferritin.
We describe an enzyme immunoassay for human serum ferritin in which antibody adsorbed on polystyrene tubes is used. Adsorbed gamma-globulins against human ferritin were first allowed to react with ferritin and a second antiferritin antibody, labeled with alkaline phosphatase, was added. The amount of bound enzyme/antibody conjugate was proportional to the ferritin titer in the assay. This method offers stable reagents that can be kept for many months at 4 degrees C. The average values for ferritin in normal men and women were, respectively, 58 and 43 microgram/liter. The lowest detectable concentration was 5 microgram/liter.
D005293:Ferritins / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007122:Immunoelectrophoresis / Q000379:methods
D005293:Ferritins