title: Incorporation of deoxynucleotides into DNA by diethylaminoethyldextran-treated lymphocytes.
abstract: In the presence of diethylaminoethyldextran cultured human lymphocytes will utilize deoxynucleotides for the synthesis of DNA, whereas in the absence of diethylaminoethyldextran no incorporation of deoxynucleotides is detected. Labeled deoxynucleoside mono-, di-, and triphosphates are incorporated into DNA at approximately the same rate. Deoxynucleotide incorporation is essentially linear for 10 min but continues at a gradually diminishing rate for an additional 20 to 50 min. The initial rate of DNA synthesis is at least 20 to 40% of the in vivo rate, and in those cells that are in S phase 0.7-1.5% of the DNA is synthesized. By the three properties examined (restriction to S phase, semiconservative mode, and initial product in short chains), DNA synthesis in diethylaminoethyldextran-treated cells resembles DNA synthesis in vivo.
mesh_terms: D002460:Cell Line; D003637:DEAE-Dextran / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D003854:Deoxyribonucleotides; D003911:Dextrans / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D003854:Deoxyribonucleotides; D003911:Dextrans; D004247:DNA; D003637:DEAE-Dextran