Functional modification of liver form-B RNA polymerase activity by a protein fraction from rats accustomed to controlled feeding schedules.
A protein fraction extracted from the liver of rats accustomed to eating during the first 8 h of a daily 12 h dark period is able to increase homologous form-B RNA polymerase activity. When the protein factor is added to RNA polymerase extracted at the end of the daily fasted period (0.9:OOh) the sensitivity of enzyme to ionic strength is modified, and resembles that of the enzyme extracted during the eating period (15:00 h) either using liver deproteinized DNA or chromatin as template. The chromatin-bound RNA polymerase activity is also modified in the presence of the factor, showing a modification of enzyme sensitivity to ionic strength similar to the one induced by food intake.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002843:Chromatin / Q000201:enzymology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004789:Enzyme Activation; D005215:Fasting; D005247:Feeding Behavior; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D011402:Pronase; D011506:Proteins / Q000502:physiology*; D012319:RNA Polymerase II / Q000378:metabolism*; D051381:Rats
D002843:Chromatin; D011506:Proteins; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D011402:Pronase