A concise method for study of the binding of thrombin to human platelets.
A rapid and sensitive technique is described for use in the study of the binding of 125I-thrombin to human platelets. The procedure involves the separation of free thrombin from platelet-bound thrombin by passage of this mixture through a discontinuous sucrose density gradient at low centrifugal force (1,500xg). Results obtained by this method are shown to be comparably to data obtained by two other conventional methods. This technique may facilitate further kinetic study of the binding of thrombin to human platelets.
D001792:Blood Platelets / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D005374:Filtration; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008722:Methods; D013917:Thrombin / Q000302:isolation & purification / Q000378:metabolism*
D007457:Iodine Radioisotopes; D013917:Thrombin