Dansyl hydrazine as a fluorimetric reagent for thin-layer chromatographic analysis of reducing sugars.
Reducing sugars, particularly aldoses, react readily with dansyl hydrazine. The fluorescent hydrazones produced can be separated by thin-layer chromatography and determined quantitatively by spectrofluorimetry after elution from the chromatograms.
D002241:Carbohydrates / Q000032:analysis*; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds; D005470:Fluorometry; D006834:Hydrazines; D008722:Methods
D002241:Carbohydrates; D003619:Dansyl Compounds; D006834:Hydrazines