A method for the simultaneous determination of serum and cell-bound IgE.
A method for determining the amount of total IgE bound to leucocytes has been devised by measuring that released on incubating various cell number dilutions in acetate buffer pH 4-0. A linear relationship between IgE released and cell number was obtained over a limited concentration range. The method seemed to work satisfactorily with 50 ml of whole blood for those subjects with very high serum levels of total IgE. However, with subjects having a slightly elevated or normal serum IgE level it may be necessary to use twice the amount of blood.
D001491:Basophils / Q000276:immunology*; D001666:Binding Sites, Antibody; D003876:Dermatitis, Atopic / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D007958:Leukocyte Count; D008722:Methods; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology
D007073:Immunoglobulin E