Effects of piperonyl butoxide on hydroxylation of 2-acetylaminofluorene in hamster liver.
Single i.p. injection of piperonyl butoxide induces a dose dependent and prolonged inhibition of hamster liver acetylaminofluorene hydroxylation. However, this slight inhibition is the result of a 61% inhibition of acetylaminofluorene-7-hydroxylase activity and a 10 fold stimulation of acetylaminofluorene N-hydroxylase activity. This was confirmed by in vivo experiments. The DNA and RNA binding of acetylaminofluorene-C14 increased 2.1 and 2.4 fold, respectively, 16 hrs after treatment with acetylaminofluorene-C14 in combination with piperonyl butoxide.
D015073:2-Acetylaminofluorene / Q000378:metabolism*; D000818:Animals; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D005449:Fluorenes / Q000378:metabolism*; D006900:Hydroxylation; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010882:Piperonyl Butoxide / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D012313:RNA / Q000378:metabolism; D013997:Time Factors
D005449:Fluorenes; D012313:RNA; D004247:DNA; D015073:2-Acetylaminofluorene; D010882:Piperonyl Butoxide