[Cornea preservation in a continuous tissue culture medium at +37 degrees C].
Seven human corneae were preserved 1 or 2 weeks in a continuous tissue culture medium at +37 degrees C. 200 microgram/ml gentamycin and 2.5 microgram/ml amphotericin B were added, and no infection was noted after 2 weeks. Histological and electronmicroscopical findings presented a picture of well-kept cellular and subcellular structures. Comparable electron-optical examinations taken on seven corneae kept at +4 degrees C in a modified medium 199 showed the beginning of endothelial necrobiosis.
D000666:Amphotericin B; D003315:Cornea / Q000648:ultrastructure; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005839:Gentamicins; D006801:Humans; D009926:Organ Preservation / Q000379:methods*; D013048:Specimen Handling / Q000379:methods; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods*
D003470:Culture Media; D005839:Gentamicins; D000666:Amphotericin B
Hornhautkonservierung bei +37 degrees C im kontinuierlichen Gewebekulturmedium.