title: A quantitative assay of mutation induction at the hypoxanthine-guanine phosphoribosyl transferase locus in Chinese hamster ovary cells (CHO/HGPRT system): utilization with a variety of mutagenic agents.
abstract: The induction of mutation by a variety of mutagens has been measured utilizing the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in Chinese hamster ovary (CHO) cells (CHO/HGPRT) system). These mutagens include physical agents such as UV light and X-rays, and chemicals such as alkylating agents, ICR-191, and metallic compounds. This system can also be modified for study of the mutagenicity of promutagens such as dimethylnitrosamine (DMN) which require biotransformation for mutagenic action, either through the addition of a rat liver microsomal activation preparation or through a host-mediated activation step using Balb/c athymic mice.
mesh_terms: D000166:Acridines / Q000494:pharmacology; D000585:Aminacrine / Q000031:analogs & derivatives; D000818:Animals; D002460:Cell Line; D004128:Dimethylnitrosamine / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008862:Microsomes, Liver / Q000378:metabolism; D009154:Mutation; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology; D051381:Rats; D014466:Ultraviolet Rays; D014965:X-Rays
chemical_list: D000166:Acridines; D009588:Nitrogen Mustard Compounds; D008769:Methylnitronitrosoguanidine; D000585:Aminacrine; C004294:acridine half-mustard; D005020:Ethyl Methanesulfonate; D007041:Hypoxanthine Phosphoribosyltransferase; D004128:Dimethylnitrosamine