Kinetics of a micelle specific palmitoyltransferase isoenzyme of rabbit mammary gland.
Palmityl-CoA: monopalmityl-sn-glycerol 3-phosphate palmitoyltransferase [EC 2.3.1. -] in rabbit mammary gland microsomes is composed of two isoenzymic species. The alpha form (LPAT-alpha) is active with monomeric substrates and inhibited by micelles while the beta form (LPAT-beta) is active only with micelles. By combining the effects of time, temperature, and Tween 80 which selectively inhibited LPAT-alpha, the substrate saturation curve for the LPAT-beta isoenzyme has been successfully determined. Both theoretical and experimental curves are in good agreement.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D005260:Female; D005989:Glycerides / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000201:enzymology*; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D010169:Palmitic Acids / Q000378:metabolism*; D011136:Polysorbates / Q000494:pharmacology; D011817:Rabbits; D013696:Temperature
D005989:Glycerides; D007527:Isoenzymes; D010169:Palmitic Acids; D011136:Polysorbates; D000217:Acyltransferases