title: Age-related changes in occurrence of Marchi-positive granules and Marchi-positive myelinoid bodies in postnatally developing feline white matter.
abstract: The occurrence of clusters of Marchi-positive granules and of Marchi-positive myelinoid bodies at different postnatal developmental stages was examined lightmicroscopically in Vibratome sections from the cervical lateral funiculus of the cat, after perfusion-fixation with glutaraldehyde. The findings show that clusters of Marchi-positive granules are most common at birth and rapidly decrease in number with development, being largely absent in animals older than 1 month. The pattern of change resembles the postnatal changes in content of esterified cholesterol in the cervical lateral funiculus and is compatible with the view that the clusters of Marchi-positive granules may result from spontaneous myelin sheath disintegration occurring early postnatally. The incidence of Marchi-positive myelinoid bodies increases 7 times during the first 4 months after birth to a peak and declines about 40% during late maturation. The size spectrum of the Marchi-positve bodies shifts markedly towards larger sizes with development and presents a close to mature picture from 120 days on. Comparisons between the Marchi-positive myelinoid bodies and the myelin sheaths in the same region, with respect to postnatal change in occurrence and size spectrum, suggest that the Marchi-positive bodies are related to myelin sheaths of large fibres or fibres destined to become large.
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002415:Cats; D002788:Cholesterol Esters / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D006651:Histocytochemistry; D050356:Lipid Metabolism; D009186:Myelin Sheath / Q000378:metabolism / Q000648:ultrastructure*; D009413:Nerve Fibers, Myelinated / Q000378:metabolism / Q000648:ultrastructure; D013116:Spinal Cord / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D002788:Cholesterol Esters