Alcohol metabolism at high alcohol concentrations.
The incorporation rate of tritium from (R,S)ethanol-1-3H, (R)ethanol-1-3H and (S)ethanol-1-3H in lactate and beta-hydroxybutyrate is investigated by means of a GLC method. Preliminary results show no decrease in incorporation of 3H from (R)ethanol during the period of labelling (15 min). These results indicate that either a shift in the isotope effect occurs at high concentrations of ethanol, or the results may indicate that ethanol does not exhibit a substrate inhibitory effect on alcohol dehydrogenase in the liver cells. Furthermore, the fraction of acetaldehyde metabolized in the cytosol is determined.
D000079:Acetaldehyde / Q000378:metabolism; D000818:Animals; D000431:Ethanol / Q000378:metabolism*; D005260:Female; D006885:Hydroxybutyrates / Q000096:biosynthesis; D066298:In Vitro Techniques; D007773:Lactates / Q000096:biosynthesis; D008099:Liver / Q000378:metabolism*; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D051381:Rats; D014316:Tritium
D006885:Hydroxybutyrates; D007773:Lactates; D014316:Tritium; D000431:Ethanol; D000079:Acetaldehyde