Development of a radioimmunoassay for aprindine.
We have developed a radioimmunoassay for aprindine, a new antiarrhythmic drug used in the treatment of ventricular disorders. The antibodies were produced by immunization of New-zealand rabbits with aprindine coupled to human serum albumin. Their biochemical characteristics have been determined. Tritiated aprindine was used as radioactive competitor. The cross-reactivity with several metabolites of aprindine was studied too. Finally, the results obtained by RIA in plasma and tissues of dogs were compared to those obtained by gas-chromatography.
D000818:Animals; D001073:Aprindine / Q000032:analysis* / Q000276:immunology; D001666:Binding Sites, Antibody; D002849:Chromatography, Gas; D003429:Cross Reactions; D004285:Dogs; D006241:Haptens; D006801:Humans; D007106:Immune Sera; D007192:Indenes / Q000032:analysis*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods
D006241:Haptens; D007106:Immune Sera; D007192:Indenes; D001073:Aprindine