[Investigation on the detection of circulating antibodies in yoshida-sarcoma bearing rats by the serologic-nephelometric method of Hoigné (author's transl)].
Circulating antibodies against tumour antigens were detected by means of the serologic-nephelometric method of Hoigné et al. (1955). Under employment of homogenates of tumour tissue as antigen solutions in 95 per cent of the cases the sera of Yoshida-sarcoma bearing rats reacted positively, while only in 4 per cent of the normal animals a positive reaction was found. 11 per cent of tumour-bearing animals showed in addition positive reactions against the normal muscle tissue, normal rats reacted positively in 2 per cent of the cases. In 93 per cent the sera of tumour-bearing animals reacted with spleen homogenates of sarcoma-bearing rats positively, whereas serum of normal rats showed 20 per cent positive reactions with the same antigen. The results confirm the aptitude of the serologicnephelometric method of Hoigné et al. (1955) and of the employed animal model for investigations of tumour-immunological problems, and they underline the antigenicity of the Yoshida-sarcoma.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm; D002474:Cell-Free System; D005260:Female; D008297:Male; D008722:Methods; D009132:Muscles / Q000276:immunology; D051381:Rats; D012517:Sarcoma, Yoshida / Q000276:immunology*; D013154:Spleen
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm
Untersuchungen zum Nachweis zirkulierender Antikörper bei Yoshida-Sarkom-tragenden Ratten mit der serologisch-nephelometrischen methode nach Hoigné.