title: Rapid serum gentamicin assay by enzymatic adenylation.
abstract: An improved, rapid and accurate enzymatic adenylation assay for gentamicin has been developed. It can be completed in one hour with an accuracy of +/- 2.7% (+/- 1 SD) for the range of gentamicin concentrations of 6-12 microgram/ml. Concentrated enzyme was used to prepare a complete adenylation mixture, which was stored frozen in aliquots suitable for daily assays. A special apparatus designed for efficient washing of phosphocellulose disks was used in the assay.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D005839:Gentamicins / Q000097:blood* / Q000378:metabolism; D006801:Humans; D013677:Medical Laboratory Science / Q000295:instrumentation; D008722:Methods
chemical_list: D005839:Gentamicins; D000255:Adenosine Triphosphate