[Covalent binding of acylated trypsin with polymeric carriers].
In order to provide covalent binding of trypsin with polymer carriers, trypsin was activated by an administration of double C=C bond. Thereafter, activated trypsin was polymerized together with a monomer of the acrylic series. Trypsin acylation with an unsaturated acid allowed the enzyme immobilization by means of its copolymerization with a monomer forming a polymer matrix. The specific proteolytic activity of immobilized trypsin decreased in proportion to an increase in the molecular weight of the polymerization product.
D000215:Acylation; D055598:Chemical Phenomena; D002621:Chemistry; D004800:Enzymes, Immobilized; D011108:Polymers; D014357:Trypsin
D004800:Enzymes, Immobilized; D011108:Polymers; D014357:Trypsin
Kovalentone sviazyvanie atsilirovammogo tripsina s polimernymi nositeliami.