Further evidence of the presence of progesterone-binding proteins in female rat hypothalamus.
Utilizing techniques of Sephadex G-200 column chromatography, sucrose density gradient ultracentrifugation and dextran-coated charcoal adsorption, the characterization of progesterone-binding proteins was attempted in cytosols obtained from the medial basal hypothalamus including the median eminence, the remaining hypothalamus and the cerebral cortex of ovariectomized estrogen-primed rats. Ater incubation of the medial basal hypothalamic cytosol with 3H-progesterone in the absence or in the presence of various unlabeled steroids, a specific progesterone-binding protein was found in the 7 S region with its binding capacity of approximately 2 X 10(14) moles per mg protein and dissociation constant of approximately 6 X 10(-9) M. No specific binding was, however, found in the remaining hypothalamus and the cerebral cortex.
D000818:Animals; D002540:Cerebral Cortex / Q000032:analysis; D003600:Cytosol / Q000032:analysis*; D005260:Female; D007031:Hypothalamus / Q000032:analysis*; D011374:Progesterone / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats
D011506:Proteins; D011374:Progesterone