title: High-speed liquid chromatographic determination of pilocarpine in pharmaceutical dosage forms.
abstract: A specific method for the direct determination of pilocarpine in aqueous pharmaceuticals in the presence of decomposition products, methylcellulose, and other ingredients usually present in pharmaceuticals is described. The method involves separation by high-speed liquid chromatography using, in series, octadecylsilane bonded to silica and cyanopropylsilane bonded to silica columns and a tetrahydrofuran-pH 9.2 borate buffer (3:7) eluant. Quantitation is achieved by monitoring the absorbance of the effluent at 254 nm and using a pyridine internal standard and a calibration curve prepared from known concentrations of pilocarpine nitrate. The reproducibility of the retention time and peak area was better than 2.0%.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D008722:Methods; D009883:Ophthalmic Solutions / Q000032:analysis; D010862:Pilocarpine / Q000032:analysis*
chemical_list: D009883:Ophthalmic Solutions; D010862:Pilocarpine