Some characteristics of the residue obtained after pronase treatment of sheep erythrocyte membranes. I. Protein and phospholipid patterns.
In this note some data regarding analysis of sheep erythrocyte stromata after pronase treatment are reported. The membrane material obtained after pronase treatment was analyzed by density gradient ultracentrifugation, gel filtration, acrylamide gel electrophoresis and for phospholipid content. With all techniques employed two major lipo or glycolipopeptides can be observed. The whole body of phospholipids is not lost after pronase treatment, therefore lipid-lipid interactions are maintained after pronase treatment, moreover sodium dodecyl sulphate is not efficient in the breakdown of some protein-lipid interactions. The results are discussed in view of the correspondence of the two fractions obtained with portions of the major glycoproteins of the erythrocyte membranes.
D000818:Animals; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D007700:Kinetics; D008563:Membrane Lipids / Q000097:blood*; D008565:Membrane Proteins / Q000097:blood*; D010743:Phospholipids / Q000097:blood*; D011402:Pronase / Q000378:metabolism; D012756:Sheep
D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D011402:Pronase