[Two simple methods for isolation of DNA from various sources using cetavlon].
Two general methods for the isolation of DNA from various sources based on the use of cetyltrimethylammonium bromide (cetavlon, CTA-Br) are described. Cetavlon is a strong cationic detergent precipitating DNA from diluted salt solutions. Cells are lysed and cellular components are dissolved in the presence of cetavlon, 5 M urea, 0.1 M EDTA and 2 M NaCl (KCl). In the first method pure DNA is precipitated in the form of CTA-salt by direct dilution of the lysate to bring the concentration of NaCl (KCl) down to 0.5 M after the removal of the main part of proteins by deproteinization with chloroform. In the first method pure DNA is precipitated in the form of CTA-salt by direct dilution of the lysate to bring the concentration of NaCl (KCl) down to 0.5 M after the removal of the main part of proteins by deproteinization with chloroform. In the second method DNA is purified on the hydroxyapatite column after cell lysis and the removal of cell debris by centrifugation. Both methods are suitable for rapid isolation of pure DNA from various sources with recovery about 80% and average molecular weight 20-10(6) and higher without use of ribonuclease, pronase and amylase.
D002593:Cetrimonium Compounds; D011232:Chemical Precipitation; D002845:Chromatography / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D006882:Hydroxyapatites; D008722:Methods
D002593:Cetrimonium Compounds; D006882:Hydroxyapatites; D004247:DNA
Dva prostykh metoda vydeleniia DNK iz razlichnykh istochnikov s primeneniem tsetavlona.