title: Purification of human fibroblast interferon by zinc chelate affinity chromatography.
abstract: Zinc chelated by iminodiacetic acid linked to an insoluble matrix binds human fibroblast interferon quite selectively at neutral pH in 0.15 M NaCL. On reduction of the pH and increase of the ionic strength, the interferon is eluted. Using a pH gradient at constant high ionic strength, good purification of the interferon can be obtained, up to a final specific activity of 108.5 units/mg of protein. Approximately 60% of the applied activity can be recovered.
mesh_terms: D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004171:Diploidy; D005347:Fibroblasts; D006801:Humans; D007372:Interferons / Q000097:blood / Q000302:isolation & purification*; D007962:Leukocytes; D008214:Lymphocytes; D015032:Zinc
chemical_list: D007372:Interferons; D015032:Zinc