Ribose 1-phosphate metabolism in Ehrlich ascites tumor cells in vitro.
Ribose 1-phosphate concentrations have been measured in tumor cells incubated with purine and pyrimidine nucleosides and with glucose. Highest concentrations (0.15 to 0.2 mumol/ml of cells) were attained in cells incubated with inosine. Although uridine was cleaved at approximately the same rate as inosine, as judged by lactate accumulation, concentrations of ribose 1-phosphate that accumulated were only approximately 0.06 mumol/ml. Ribose 1-phosphate accumulation in tumor cells incubated with inosine was dependent on the phosphate concentration of the medium up to at least 25 mM. Ribose 1-phosphate formed from inosine was readily converted both to phosphoribosyl pyrophosphate and to lactate.
D000818:Animals; D001692:Biological Transport; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D010428:Pentosephosphates / Q000378:metabolism*; D011684:Purine Nucleosides / Q000494:pharmacology; D011741:Pyrimidine Nucleosides / Q000494:pharmacology; D012267:Ribosemonophosphates / Q000378:metabolism*
D010428:Pentosephosphates; D011684:Purine Nucleosides; D011741:Pyrimidine Nucleosides; D012267:Ribosemonophosphates; D005947:Glucose