[Production of carbon-labelled rubomycin by means of biosynthesis].
The authors obtained 14C-rubomycin biosynthetically with the use of 14C-acetate, 14C-propionate and methionine (methyl-14C) as the label source. It was shown that radiochemically pure 14C-rubomycin may be obtained from a non-purified preparation of radioactive glucose, i. e. a preparation obtained by hydrolysis of chlorella cells grown on a nitrogen-free medium in an atmosphere of 14CO2. It was found that the use of soyabeen meal + glyserol medium for synthesis of the labeled rubomycin from 14C-glucose resulted in a two-fold increase in the specific activity of the antibiotic.
D002250:Carbon Radioisotopes; D002708:Chlorella; D003470:Culture Media; D003630:Daunorubicin / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods; D011492:Protein Hydrolysates / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism; D013997:Time Factors
D002250:Carbon Radioisotopes; D003470:Culture Media; D011492:Protein Hydrolysates; D005947:Glucose; D005990:Glycerol; D003630:Daunorubicin
Poluchenie mechennogo uglerodom rubomitsina metodom biosinteza.