Evaluation of liquid scintillation counting solutions for radioimmunoassay of hepatitis-associated antigen (HBsAg).
Liquid scintillation counting solutions for radioimmunoassay of hepatitis associated antigen (HBsAg) are evaluated and discussed. Data is also presented which indicates that liquid scintillation counting could be carried out by placing cut-off Ausria-125 test tubes in counting vials containing 10 ml of either Brays, Unogel, or Instagel solutions. The data suggest that Brays was the least effective and Unogel and Instagel were the most effective liquid scintillation counting solutions. This is a fundamental consideration if a laboratory is contemplating an initial investiment in counting equipment. The acquisition of a liquid scintillation counter provides additional capabilities for the clinical laboratory interested in radioisotope measurements. In addition, if a laboratory already has a liquid scintillation counter (Beta counter), the director of the clinical laboratory may elect not to purchase additional counting equipment (gamma counter) since he already has the added advantage of measuring iodine-125 with the Beta counter. The authors propose liquid scintillation (RIA) as an alternative counting system for HBsAg detection in hospital patients and donors.
D005188:False Negative Reactions; D005189:False Positive Reactions; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods; D012588:Scintillation Counting; D012996:Solutions
D006514:Hepatitis B Surface Antigens; D012996:Solutions