In vitro uptake of gentamicin by rat renal cortical tissue.
The mechanism of gentamicin uptake in vitro by renal cortical slices of rat kidney was investigated. The cortical-slice-uptake ratio of gentamicin concentration in 1.0 g of tissue water to that of 1.0 ml of incubation medium (SW/M) was 1.44 +/- 0.04. The uptake of gentamicin was inhibited by 2 x 10(-5) M dinitrophenol (SW/M = 1.03 +/- 0.04) and by anoxia (SW/M = 1.01 +/- 0.04). The results indicate that aerobic phosphorylation is required to transport gentamicin into the cells. The uptake of p-aminohippurate and tetraethylammonium chloride by renal cortical slices was not affected by gentamicin.
D000818:Animals; D005839:Gentamicins / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013757:Tetraethylammonium Compounds / Q000378:metabolism; D014867:Water / Q000378:metabolism; D010130:p-Aminohippuric Acid / Q000378:metabolism
D005839:Gentamicins; D013757:Tetraethylammonium Compounds; D014867:Water; D010130:p-Aminohippuric Acid