The isolation of a suppressible nonsense mutant in mammalian cells.
An HGPRT- cell line derived from mouse L cells has been shown to have the following properties: it is CRM+; the defective HGPRT molecules are altered in the carboxyterminal peptide; the mutant cells regain HGPRT activity when ochre-suppressor tRNA is microinjected into them, but not when amber-suppressor or wild-type tRNAs are injected. We conclude from these properties that this mutant cell line contains an ochre nonsense mutation (UAA) in the structural gene for HGPRT.
D002460:Cell Line; D005796:Genes; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis / Q000096:biosynthesis*; D008845:Microinjections; D009154:Mutation; D010455:Peptides / Q000032:analysis; D012343:RNA, Transfer / Q000378:metabolism; D013489:Suppression, Genetic
D010455:Peptides; D012343:RNA, Transfer; D007041:Hypoxanthine Phosphoribosyltransferase