Differential RNA metabolism in rat brain cells fractionated by zonal centrifugation.
Combined fractionation and RNA metabolism studies were made on homoiogous cell types (neurons and glia) banded from rat brain cerebral cortex and hippocampus tissue at 40,000 rev/min for 50 minutes in a Beckman Ti 14 zonal rotor loaded with a discontinuous methyl cellulose (Methocel) and sucrose density gradient. Comparisons were made on the cellular fractions recovered from animals intracisternally injected with 15 muCi tritiated cytidine (3HCR) RNA precursor one hour before sacrifice and tissue cellular filtrate preparation. Immediate 3H pool-correction of extracted 3HRNA from the three cellular Bands recovered made possible a mathematically valid measure of 3HCR-precursor incorporation into RNA of the different cell types. Comparison of the cellular 3HRNA data by analysis of variance revealed the occurrence of differential tritium labelling of RNA in the same cell types banded from different brain regions. In particular, an important advance was demonstrated by the procedures developed in these studies for the quantitative cellular assay of brain in vivo (differential) RNA metabolism.
D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002501:Centrifugation, Zonal; D002540:Cerebral Cortex / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D012313:RNA / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism
D012313:RNA