Differences in rotation-mediated aggregation between wild-type and homozygous Brachyury (T) cells.
Rotation-mediated aggregation was studied in dissociated cells from 9- and 10-day-old ('12-40 somite' stage) mouse embryos homozygous for the Brachyury (T) mutation. Average diameter of the aggregates formed by wild-type cells increased up to the 27-somite stage. In T/T cells, however, a completely different pattern was observed. The disappearance of free cells and the process of aggregate formation were also followed. The possibility that T/T cells have different adhesive properties from +/+ cells is discussed.
D000818:Animals; D002449:Cell Aggregation; D002452:Cell Count; D002469:Cell Separation; D005865:Gestational Age; D066298:In Vitro Techniques; D051379:Mice; D009154:Mutation