title: Proteins from disassembled microtubules characterized by oligospecific antisera.
abstract: The immunochemical properties of in vitro reassembled microtubules were investigated by immunoelectrophoretic techniques. The tubulin dimer gave no measurable immunochemical response, but the tubulin oligomer, the tau-factor and an antigen of about 135 000 daltons all gave precipitating antibodies. Those four proteins were investigated in reassembled microtubules, in DEAE-cellulose purified tubulin, and after molecular sieve chromatography of disassembled and NaCl-dissociated microtubules. Reconstitution of tubulin oligomer from tubulin dimer and tau-factor was also performed. The presence of a unique antigenic structure on tubulin oligomer which was not found in the dissociated components and the role of this aggregate as a nucleation center or intermediate in the assembly of microtubules is discussed.
mesh_terms: D000818:Animals; D001921:Brain / Q000276:immunology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D007123:Immunoelectrophoresis, Two-Dimensional; D008870:Microtubules / Q000276:immunology* / Q000648:ultrastructure; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000276:immunology; D051381:Rats; D014404:Tubulin / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D006023:Glycoproteins; D009419:Nerve Tissue Proteins; D014404:Tubulin