[Changes in the isoenzymatic spectrum of energy metabolism enzymes and in the mitochondrial ultrastructure in viral carcinogenesis].
The dynamics of redistribution of the isoenzymatic spectrum of lactate and malate dehydrogenases in the cells of rat embryo fibroblasts (REF) having been in contact with human adenovirus type 12, as compared to the dynamics of changes in the fine structure of the cellular mitochondria of the same models was studied. The action of oncogenic virus caused rearrangment in the isoenzymatic spectra of lactate and malate dehydrogenases both in the nuclei and in the cytoplasm. These disturbances occur from the first days of the REF culture interaction with the oncogenic virus and are not connected with the proliferative growth of the cell culture, but they are stipulated by the very fact of the virus influence on the cell. Comparison of the kinetics of the changes in the enzymes under study with the data of quantitative analysis of the fine structure of the mitochondrial apparatus demonstrated that biochemical shifts occurred much earlier, practically from the moment of infecting.
D000260:Adenoviruses, Human / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003593:Cytoplasm / Q000201:enzymology; D004734:Energy Metabolism; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000201:enzymology / Q000209:etiology*; D051381:Rats; D013997:Time Factors
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase
Izmeneniia izofermentnogo spektra nekotorykh fermentov énergeticheskogo obmena i ul'trastruktury mitokhondriĭ pri virusnom kantserogeneze.