Murine colon adenocarcinoma: syngeneic orthotopic transplantation and subsequent hepatic metastases.
Syngeneic murine colon adenocarcinoma (MCA-38) cells were transplanted in the submucosa of distal colon, proximal colon, cecum, ileum, jejunum, and duodenum of male C57BL/6 mice, with local lymphoid follicles used as points of entry. The tumor grew best at the cecum and led to liver and mesenteric lymph node metastases in 8 and 9 weeks, respectively, after transplantation. Histologically, a local inflammatory reaction involving polymorphonuclear leukocytes was observed within 48-72 hours following transplantation; after this time, the microscopic tumor foci began to grow progressively. Mononuclear lymphoid cells of the gut-associated lymphoid tissue did not infiltrate the progressively growing tumor; however, polymorphonuclear leukocytes were constantly observed at the tumor periphery in the lamina propria. The studies indicated that orthotopic transplantation as a model system can provide a means of examining the role of the local immune response as a focus of host resistance and as a factor in metastatic tumor spread. The findings also suggested the usefulness of this model in immunotherapeutic and chemotherapeutic studies of secondary hepatic disease.
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D002430:Cecal Neoplasms / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology*; D004379:Duodenal Neoplasms / Q000473:pathology; D007082:Ileum; D007414:Intestinal Neoplasms / Q000473:pathology; D007583:Jejunum; D008113:Liver Neoplasms / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009504:Neutrophils / Q000473:pathology; D014185:Transplantation, Isogeneic