The enzymic derivation of citrulline residues from arginine residues in situ during the biosynthesis of hair proteins that are cross-linked by isopeptide bonds.
An enzymic activity present in hair follicles is described that can convert arginine residues to citrulline residues in proteins in situ. The Ca2+ dependent enzyme activity has been detected in hair follicle extracts but not in similar extracts of serum, liver or brain. The enzyme appears to act on proteins other than hair proteins and the citrulline produced can be quantitated in acid hydrolysates by a colorimetric procedure. The formation of citrulline has been confirmed by amino acid analysis and does not  appear to be related to the formation of isopeptide linkages which is catalysed by the transamidase present in hair follicles.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001120:Arginine / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002956:Citrulline / Q000378:metabolism; D006197:Hair / Q000378:metabolism*; D007633:Keratins; D007700:Kinetics; D046911:Macromolecular Substances; D014176:Protein Biosynthesis; D011506:Proteins
D000596:Amino Acids; D046911:Macromolecular Substances; D011506:Proteins; D002956:Citrulline; D007633:Keratins; D001120:Arginine; D002118:Calcium