An investigation into the immunochemical properties of the isoenzymes of human alkaline phosphatase.
A study was carried out to investigate the immunochemical properties of the alkaline phosphatases present in liver, placenta, intestine, and kidney. Butanol extracts of the tissues were examined by antigen-antibody crossed electrophoresis, and the immunoprecipitates formed by the enzymes were identified by use of a specific stain for alkaline phosphatase. The results obtained revealed three immuno-chemical types of alkaline phosphatase, designated L, P, and PI respectively. The distribution of these enzymes among the tissues examined was as follows: type L was the only enzyme detected in liver extract and type P the only one detected in placental extract. The extracts of intestine and kidney both contained two isoenzymes, one of which was type L and the other type PI.
D000469:Alkaline Phosphatase / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000276:immunology* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D009928:Organ Specificity; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy
D007527:Isoenzymes; D000469:Alkaline Phosphatase