title: DNA synthesis in immunreactive glandular cells in pituitary intermediate lobe cultures.
abstract: Cell suspensions were prepared from adult rat pituitary intermediate lobes and grown in medium TC 199 supplemented with foetal calf serum. 7 or 8 days old cultures were pulse labelled with tritiated thymidine and thereafter processed for immunostaining using an antiserum against a synthetic alpha-1-28-ACTH-analogue. Immunreactivity was mainly confined to epithelial cells, but some spindle-shaped cells were also immunopositive. Thymidine incorporation was observed in some immunpositive cells which shows that cells committed to hormone production may enter the mitotic cycle.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D010902:Pituitary Gland / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats
chemical_list: D004247:DNA