Complex mitochondrial DNA in Drosophila.
The larval mtDNA isolated from D. virilis, D. simulans and D. melanogaster exists in complex molecular forms in addition to the simple monomeric circular form. The frequency of circular dimers and oligomers is highly elevated in apparently normal larval tissues. These complex forms of mtDNA are separable on agarose gels. Hind III restriction endonuclease and electron microscopic analyses used in the present study have revealed that circular dimers are simply the circular concatemers of two monomeric circles which are arranged in a head-to-tail structure with no detectable heterologous regions such as insertions or deletions. The electrophoretic patterns of Hind III digested mtDNAs of D. simulans and D. melanogaster (sibling species) are identical and distinguishable from that of distantly related species, D. virilis.
D000818:Animals; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000302:isolation & purification; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D004330:Drosophila; D007814:Larva; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009695:Nucleic Acid Renaturation; D013045:Species Specificity
D004270:DNA, Circular; D004272:DNA, Mitochondrial; D046911:Macromolecular Substances; D004262:DNA Restriction Enzymes