Immune RNA-mediated transfer of tumor antigen responsiveness to unresponsive peritoneal exudate cells from tumor-bearing animals.
Peritoneal exudate cells (PEC) from mice inoculated 5 to 7 days previously with 1 X 10(6) MOPC-315 plasmacytoma cells exhibit in vitro migration-inhibitory factor reactivity to soluble tumor-associated antigens. By 10 to 14 days of tumor growth, PEC from MOPC-315-bearing mice did not elicit migration-inhibitory factor when stimulated with MOPC-315 tumor-associated antigens but were still capable of migration-inhibitory factor production when stimulated with nontumor antigens. RNA-rich extracts prepared from 5- and 6-day postgrafting tumor bearers were capable of transferring tumor antigen reactivity to both normal PEC and PEC from unresponsive MOPC-315-bearing mice. On the other hand, RNA from unresponsive tumor bearers was incapable of transferring tumor antigen reactivity to normal mouse cells.
D000818:Animals; D000951:Antigens, Neoplasm; D001202:Ascitic Fluid / Q000276:immunology*; D002464:Cell Migration Inhibition; D005260:Female; D007111:Immunity, Cellular; D007116:Immunization, Passive; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology; D010954:Plasmacytoma / Q000276:immunology*; D012334:RNA, Neoplasm / Q000276:immunology*; D013997:Time Factors; D014185:Transplantation, Isogeneic
D000951:Antigens, Neoplasm; D012334:RNA, Neoplasm