[Isolation and characterization of pigment-protein complexes from green serobacteria Chlorobium limicola forma thiosulfatophilum].
A subchromatophore fraction containing the reaction center P-840 was isolated from the chromatophores of green serobacteria Chlorobium limicola forma thiosulfatophilum by ultracentrifugation and its protein composition was characterized. After treatment of the chromatophores by Triton X-100 and subsequent polyacrylamide gel electrophoresis two pigment-protein complex containing bacterioviridin. The proteins of the pigment-protein complex containing bacterioviridin. The proteins of the pigment-protein complexes were obtained. The first complex contained mainly bacteriochlorophyll alpha with bacterioviridin contaminations and the second one-only bacterioviridin. The low-temperature absorption spectrum of the bacteriochlorophyll alpha-containing pigment-protein complex is identical to the absorption spectrum of a water-soluble pigment-protein complex isolated and characterized by Olson and contains proteins with molecular weights of 34.800, 33.100, 27.500 and 23.200. A protein with molecular weight of 32.700 was found in the pigment-protein complexes were compared to the proteins of the photochemically active subchromatophore fraction and chromatophores.
D020130:Bacterial Chromatophores / Q000032:analysis*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D001429:Bacteriochlorophylls / Q000302:isolation & purification; D002458:Cell Fractionation; D008970:Molecular Weight; D010860:Pigments, Biological / Q000302:isolation & purification*; D012245:Rhodospirillales / Q000032:analysis*
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D010860:Pigments, Biological; C002084:bacteriochlorophyll c
Vydelenie i kharakteristika pigment-belkovykh kompleksov iz zelenoĭ serobakterii Chlorobium limicola forma pigment-belkovykh kompleksov iz zelenoĭ serobakterii Chlorobium limicola forma thiosulfatornilum.