Triiodothyronine and thyroxine content of desiccated thyroid tablets.
Triiodothyronine (T3) and thyroxine (T4) were measured by radioimmunoassay in Pronase hydrolysates of four lots each of 1- and 2-grain tablets of desiccated thyroid (Thyroid, Armour) and thyroglobulin (Proloid, Warner-Chilcott). The methodology used was verified by studies of tablets containing known quantities of T4 and T3. One grain of desiccated thyroid contained 12 +/- 1 and 64 +/- 3 microgram (mean +/- SD) of T3 and T4 per tablet, respectively (T4/T3 molar ratio, 4.3). A 1-grain tablet of thyroglobulin contained 16 +/- 2 and 55 +/- 5 microgram of T3 and T4, respectively with a T4/T3 ratio of 2.9. Two-grain tablets generally contained twice the quantity of T3 and T4 in the 1-grain preparations. The variation in T3 and T4 content between the four lots of each tablet strength for each product was 10% or less. These estimates of T3 and T4 content are 1.5- to 2-fold greater than those previously published. This difference probably results from the more sophisticated methodology now available which does not require chromatographic separation of T3 and T4 or iodometry. Using calculations based on published estimates of T4 and T3 absorption and of the T3/T4 potency ratio, it would appear that the T3 content of desiccated thyroid and thyroglobulin provide approximately 39% and 51%, respectively, of the thyromimetic activity of these two medications.
D002626:Chemistry, Pharmaceutical; D011786:Quality Control; D011863:Radioimmunoassay; D013607:Tablets; D013962:Thyroid (USP) / Q000032:analysis*; D013963:Thyroid Hormones / Q000032:analysis*; D013974:Thyroxine / Q000008:administration & dosage / Q000032:analysis*; D014284:Triiodothyronine / Q000008:administration & dosage / Q000032:analysis*
D013607:Tablets; D013962:Thyroid (USP); D013963:Thyroid Hormones; D014284:Triiodothyronine; D013974:Thyroxine