[Fluorescent-cytochemical study of the action of benz(a)pyrene on subcutaneous connective tissue cells during malignant transformation in vitro].
Alterations in the secondary DNA structure of cell nuclei of the rat's subcutaneous connective tissue exposed to benz(a)pyrene (BP) action have been revealed using the Roshlay fluorescent-cytochemical method in cultivating in vitro. Alterations in the luminescence of the acridin orange complex with DNA, i.e. displacement towards the red region of spectrum, indicates the changes in the secondary structure of DNA. The strongest action on the primary cultures were noted after the injection of carcinogen (on the 3rd day of explanation). The toxical effect was lower after a repeated BP injection (on the 10th and 20th days of explanation). Cells undergone malignization and transformation, due to the BP action on the primary explantates, were more resistant to carcinogen action than the normal cells. However, the spontaneously malignized cells were more sensitive to the BP action than the cells transformed under the carcinogen action.
D000818:Animals; D001580:Benzopyrenes; D002273:Carcinogens; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D003239:Connective Tissue Cells; D004247:DNA; D066298:In Vitro Techniques; D008968:Molecular Conformation; D051381:Rats
D001580:Benzopyrenes; D002273:Carcinogens; D004247:DNA
Fluorestsentno-tsitokhimicheskoe izuchenie deĭstviia benz(a)pirena na kletki podkozhnoĭ soedinitel'noĭ tkani v protssesse malignizatsii vne organizma.