Polyamine metabolism II: N-(Monoaminoalkyl)- and N-(polyaminoalkyl)acetamides in human urine.
TLC and high-pressure liquid chromatographic examination of the dansyl derivatives obtained from human urine indicated the presence of N-[3-[4-aminobutyl)amino]propyl]acetamide (N1-acetylspermidine), N-[4-](3-aminopropyl)amino]buryl]acetamide (N8-acetylspermidine), N-(4-aminobutyl)acetamide (N-acetylputrescine), and N-(5-aminopentyl)acetamide (N-acetylcadaverine). The ratio of N1- to N8-acetylspermidine ranged from 10.3 in the urine of a patient with hepatoma to 1.1 in the urine of a normal subject. The three cancer patients had a considerably higher ratio of N1- to N8-acetylspermidine than did the three normal subjects. These findings indicate the ratio of N1- to N8-acetylspermidine in the 24-hr urine may serve as a biochemical marker for cancer.
D000081:Acetamides / Q000652:urine; D000328:Adult; D002648:Child; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds; D006801:Humans; D009369:Neoplasms / Q000652:urine; D011073:Polyamines / Q000652:urine*
D000081:Acetamides; D003619:Dansyl Compounds; D011073:Polyamines