A rapid mini-thin-layer chromatographic procedure for the detection of barbiturates and common sedatives at overdose levels.
A simple, rapid thin-layer chromatographic method is presented for the detection of barbiturates in blood, serum, or urine in overdose situations. Extraction with ether is followed by evaporation and reconstitution with methanol. Part of the extract is spotted on a 2.5 multiplied by 7.5 cm thin-layer chromatographic plate which is then developed in a mixed solvent system. After drying, the plate is sprayed with n, 2, 6-trichloro-p-benzoquinone imine and heated. The presence of a barbiturate is indicated by a blue spot. Chromatography in another solvent system assists in the identification of the particular barbiturate ingested.
D001463:Barbiturates / Q000097:blood / Q000506:poisoning* / Q000652:urine; D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood / Q000506:poisoning* / Q000652:urine
D001463:Barbiturates; D006993:Hypnotics and Sedatives