Selective plasmid transduction in Bacillus pumilus.
The inducible temperate bacteriophage phi75 and a clear-plaque-forming variant, phi75C1, mediated transduction of a 4.4 X 10(6)-dalton multicopy Bacillus pumilus plasmid, pPL10, at frequencies of 10(-5) to 10(-6) transductants per plaque-forming unit. phi75- and phi75C1-mediated transduction of several chromosome markers tested did not occur at a detectable frequency. phi75-mediated plasmid transducing activity resides in particles that are similar to infectious particles in sedimentation velocity and buoyant density.
D001407:Bacillus; D001435:Bacteriophages / Q000254:growth & development; D005111:Extrachromosomal Inheritance; D008242:Lysogeny; D008937:Mitomycins / Q000494:pharmacology; D010957:Plasmids; D014161:Transduction, Genetic
D008937:Mitomycins