Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma.
A method was developed to separate and quantitatively determine two different triglyceride lipase activities in human postheparin plasma: hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL). Affinity chromatography on heparin-Sepharose columns was used for the separation. Rechromatography of purified H-TGL on heparin-Sepharose resulted in recoveries of 74 and 97% of these enzyme activities, respectively. The analytical errors for the determinations of the two activities were 11.4 and 9.6%, respectively.
D005260:Female; D006493:Heparin; D006801:Humans; D008049:Lipase / Q000097:blood* / Q000302:isolation & purification; D008071:Lipoprotein Lipase / Q000097:blood* / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology*; D014280:Triglycerides
D014280:Triglycerides; D006493:Heparin; D008049:Lipase; D008071:Lipoprotein Lipase