A simple procedure for the isolation of L-fucose-binding lectins from Ulex europaeus and Lotus tetragonolobus.
L-Fucose-binding lectins from Ulex europeaus and Lotus tetragonolobus were isolated by affinity chromatography on columns of L-fucose-Sepharose 6B. L-Fucose was coupled to Sepharose 6B after divinyl sulfone-activation of the gel to give an affinity adsorbent capable of binding more than 1.2 mg of Ulex lextin/ml of gel, which could then be eluted with 0.1M or 0.05M L-fucose. Analysis of the isolated lectins by hemagglutination assay, by gel filtration, and polyacrylamide disc-electrophoresis revealed the presence of isolectins, or aggregated species, or both. The apparent mol. wt. of the major lectin fraction from Lotus was 35000 when determined on Sephadex G-200 or Ultrogel AcA 34. In contrast, the apparent mol. wt. of the major lectin fraction from Ulex was 68 000 when chromatographed on Sephadex G-200 and 45 000 when chromatographed on Ultrogel AcA 34. The yields of lectins were 4.5 mg/100 g of Ulex seeds and 394 mg/100 g of Lotus seeds.
D000017:ABO Blood-Group System; D002846:Chromatography, Affinity / Q000379:methods; D005643:Fucose; D006386:Hemagglutination Tests; D037102:Lectins / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008970:Molecular Weight; D037121:Plant Lectins; D012639:Seeds; D013045:Species Specificity
D000017:ABO Blood-Group System; D037102:Lectins; D046911:Macromolecular Substances; D037121:Plant Lectins; D005643:Fucose