Electron microscopic studies of purified eukaryote messenger RNA.
Rat liver mRNA, hen and rabbit globin messenger RNA (mRNA) were investigated by electron microscopy. No secondary structures were visible in the molecules of these mRNAs under conditions where short secondary structures of other types of RNA, i.e. MS2 phage RNA and 28 S rRNA, were clearly demonstrated. The contour lengths of these mRNAs were also determined by electron microscopy and compared with the sizes estimated by other techniques. The contour lengths of rabbit short and long globin mRNA are 0.1498 +/- 0.0019 and 0.1908 +/- 0.0021 micrometer, respectively. The former is assumed to be globin mRNA for alpha chain and the latter for beta chain. Similarly, hen "alpha" and "beta" globin mRNA have mean lengths of 0.1449 +/- 0.0011 and 0.1891 +/- 0.0017 micrometer, respectively. Hen and rabbit reticulocytes contain 1.8-2.0 times as much mRNA for alpha globin chains as for beta.
D000818:Animals; D002645:Chickens; D005260:Female; D005914:Globins / Q000096:biosynthesis; D008099:Liver / Q000032:analysis*; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000032:analysis* / Q000378:metabolism; D013045:Species Specificity
D012333:RNA, Messenger; D012367:RNA, Viral; D005914:Globins