[Determination of lactate with centrifugal analyser (Gemsaec) by lactate dehydrogenase in the presence of alanine aminotransferase].
An automated method for lactat determination on centrifugal analyser is described. The measurements have been done by end-point method during 300 seconds. A reagent blank is introduced in each run and its absorbance is deduced from those of the tests. The suitability of the method is assessed, particularly the sensitivity (detection limit) and the accuracy by checking the recovery. The results obtained by the automated and the manual method are well correlated.
D000410:Alanine Transaminase; D001322:Autoanalysis / Q000379:methods; D002498:Centrifugation; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase; D007773:Lactates / Q000097:blood*; D011786:Quality Control
D007773:Lactates; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase
Détermination du lactate sur analyseur centrifuge (Gemsaec) par la lactate déhydrogénase en présence d'alanine aminotransférase.