title: Isolation of reconstitutively active succinate dehydrogenase in highly purified state.
abstract: Existing procedures for the isolation of mammalian succinate dehydrogenase yield preparations of high purity or retain reconstitution activity, but not both. A new procedure is described for the isolation in good yield of virtually homogeneous preparations with full reconstitution activity, and retaining iron-sulfur center 3 and the "low Km" reaction site of ferricyanide. On reincorporation of the soluble enzyme into alkali-treated membranes, the same high turnover number (approximately 21,000/min at 38 degrees) is obtained in catalytic assays as with intact inner membrane preparations.
mesh_terms: D000818:Animals; D008566:Membranes / Q000201:enzymology; D008722:Methods; D008928:Mitochondria / Q000201:enzymology; D013385:Succinate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D013385:Succinate Dehydrogenase