Localizing of C-reactive protein in synovium of patients with rheumatoid arthritis.
Synovial biopsies from 8 patients with rheumatoid arthritis, 2 patients with degenerative osteoarthritis, and 4 patients with nonarthritic disease were studied for localization of C-reactive protein (CRP) using immunofluorescence microscopy. The nuclei of many synoviocytes and histiocytes in rheumatoid synovial membrane were found to bind CRP. Cultures of rheumatoid synovium in 14C-labeled amino acids produced radioactive IgG, IgM, IgA, and C3; but not CRP, indicating the synovial-bound CRP was not of local origin. A few CRP-binding nuclei were present in one osteoarthritis synovium, but none was found in the other and none in synovium from the 4 nonarthritis patients. The nature of the nuclear CRP ligand in rheumatoid synovium was not determined.
D000328:Adult; D001171:Arthritis, Juvenile / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000378:metabolism*; D002097:C-Reactive Protein / Q000032:analysis* / Q000276:immunology; D002467:Cell Nucleus / Q000378:metabolism; D002648:Child; D003176:Complement C3 / Q000032:analysis; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D006644:Histiocytes / Q000648:ultrastructure; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008024:Ligands; D008297:Male; D008875:Middle Aged; D011485:Protein Binding; D013583:Synovial Membrane / Q000378:metabolism* / Q000648:ultrastructure
D003176:Complement C3; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008024:Ligands; D002097:C-Reactive Protein