Chemical studies on a hamster sublingual glycoprotein.
A secretory glycoprotein was isolated from the hamster sublingual gland. It contains 65% carbohydrates, the remainder being protein. The principal type of sialic acid was identified as N-acetylneuraminic acid with about 1/4 of the molecules O-acetylated. The hexosamine occurs mainly as N-acetylgalactosamine; other sugars present are galactose and fucose. The carbohydrate side chains are linked to the protein core by an O-glycosyl linkage between seryl and threonyl residues and N-acetylgalactosamine. The glycoprotein has an apparent molecular weight of 330,000. The sialogly coprotein may act as a biological antifreeze. Relevant differences in the chemical composition of the secretory glycoproteins of the sublingual and submaxillary salivary glands of this species are briefly discussed.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002498:Centrifugation; D006224:Cricetinae; D005615:Freezing; D012794:Sialic Acids / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis* / Q000302:isolation & purification; D013361:Sublingual Gland / Q000032:analysis* / Q000378:metabolism
D000596:Amino Acids; D002241:Carbohydrates; D012794:Sialic Acids; D012795:Sialoglycoproteins