title: Base stimulation of phospholipid metabolism in neuroblastoma cells. I. Kinetics of incorporation of N-methylated ethanolamine bases.
abstract: The effects of nitrogen bases on the regulation of phospholipid metabolism in neuroblastoma cell cultures were investigated. An increase in the total cellular phospholipids was observed up to 24 h following plating. Addition of monomethyl- and dimethylethanolamine bases resulted in a stimulation of the synthesis of their corresponding phospholipids. The average rates of synthesis of phosphatidylmonomethyl- and phosphatidyldimethylethanolamine were 0.09 and 0.12 nmol/microgram DNA per h, respectively. The labeling patterns of the various phospholipid species from ortho[32P]phosphate have been determined. They suggest that the synthesis of the analogs proceeded entirely via a phosphate mediated pathway rather than through a base exchange mechanism. A number of distinct patterns for the incorporation of bases into acyl-, alkyl- and alkenyl-containing phosphoglyceride species were indicated. The polar head group composition appeared to be intimately related to the type of bond of the hydrocarbon residue.
mesh_terms: D002460:Cell Line; D004273:DNA, Neoplasm / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism*; D007700:Kinetics; D009447:Neuroblastoma; D010743:Phospholipids / Q000096:biosynthesis*; D013329:Structure-Activity Relationship
chemical_list: D004273:DNA, Neoplasm; D004983:Ethanolamines; D010743:Phospholipids