[Low temperature and cryoprotectant effect on oxygen uptake in rat kidney cortex homogenates].
Respiration and oxidative phosphorylation of the rat kidney cortex mitochondria are studied as affected by low temperatures and cryoprotectants. There is no change in respiration in state 2 during freezing and thawing of the rat kidney cortex slices both in case of using the succinate oxidation substrate and alpha-ketoglutarate. Respiration in state 3 is inhibited in the medium with alpha-ketoglutarate during freezing under protection of polyethylene oxide-100 (PEO-100) and glycerol, and does not significantly change in case of using dimethyl sulphoxide (DMSO). The effectiveness of cryoprotectants used in the experiments reduces in the following sequence: DMSO--glycerol--PEO-100. Preservation of the main mitochondria function, ATP synthesis, is shown to be possible for the kidney cortex slices exposed to freezing and thawing.
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D007656:Ketoglutaric Acids / Q000378:metabolism; D007672:Kidney Cortex / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship; D013386:Succinates / Q000378:metabolism
D003451:Cryoprotective Agents; D007656:Ketoglutaric Acids; D013386:Succinates; D011092:Polyethylene Glycols; D000255:Adenosine Triphosphate; D005990:Glycerol; D004121:Dimethyl Sulfoxide
Vpliv niz'kikh temperatur ta krioprotektoriv na spozhivannia kisniu v gomogenatakh kori nirok shchuriv.