title: Quantitation of water-soluble acylcarnitines and carnitine acyltransferases in rat tissues.
abstract: The water-soluble acylcarnitines isolated from rat heart, skeletal muscle, liver, and testis have been characterized. The following acyl residues derived from the acylcarnitine fraction were found: acetyl, propionyl, isobutyryl, butyryl, alpha-methylbutyryl, isovaleryl, tiglyl, caproyl, beta-methylcrotonyl and methacrylyl. The amounts of these acylcarnitines in heart, liver, testis and skeletal muscle from fed rats were determined. Acetylcarnitine was the most abundant acylcarnitine; however, appreciable quantities of propionyl-, isobutyryl-, isovaleryl-, and tiglyl-carnitine were found. The levels of carnitine octanyltransferse, carnitine acetyltransferase and carnitine palmityltransferase activities were determined in several tissues. In addition, carnitine isovaleryltransferase and isobutyryltransferase activities were measured in heart, skeletal muscle, liver, testis and kidney. In all instances the specific activity of isobutyryltransferase was similar to the specific activity of acetyltransferase. The results are consistent with the proposal that carnitine is involved in the catabolism of branched-chain amino acids.
mesh_terms: D000215:Acylation; D000217:Acyltransferases / Q000032:analysis*; D000818:Animals; D002331:Carnitine / Q000031:analogs & derivatives* / Q000032:analysis; D002333:Carnitine Acyltransferases / Q000032:analysis*; D005232:Fatty Acids, Volatile / Q000032:analysis; D008297:Male; D009132:Muscles / Q000032:analysis; D009206:Myocardium / Q000032:analysis; D051381:Rats; D012995:Solubility; D013737:Testis / Q000032:analysis
chemical_list: D005232:Fatty Acids, Volatile; D000217:Acyltransferases; D002333:Carnitine Acyltransferases; D002331:Carnitine