A simple technique for studying immunoglobulin synthesis by normal and malignant plasma cells in vitro.
Plasma cells from human marrows are saturated with C-14 labelled amino acids, harvested and recultured in unlabelled growth medium. The appearance of radioactivity in the growth medium then provides a simple and rapid measure of protein synthesis. The secreted radio-labelled material is characterized by isoelectric focusing and autoradiography in acrylamide gels, a technique which has advantages over established serological methods.
D001345:Autoradiography; D002478:Cells, Cultured; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007136:Immunoglobulins / Q000096:biosynthesis*; D007525:Isoelectric Focusing; D009101:Multiple Myeloma / Q000276:immunology; D010950:Plasma Cells / Q000276:immunology*; D010954:Plasmacytoma / Q000276:immunology*
D007074:Immunoglobulin G; D007136:Immunoglobulins