The incorporation of [14C]glucosamine into dolichol diphosphate N-acetyl[14C] glucosamine by unbroken liver cells in culture.
Incubation of whole Chang liver cells with D-[1-14C]glucosamine results in incorporation of radioactivity into both proteins and lipids. A minor (approximately 3%) amount of the labelled lipid has the chromatographic, solubility and chemical properties of dolichol diphosphate N-acetylglucosamine. A similar compound is formed when membrane preparations of the cells are incubated with UDP-N-acetyl[14C]glucosamine. The same membrane fractions catalyse the transfer of [14C]-mannose from GDP-[14C]mannose to dolichol phosphate.
D000117:Acetylglucosamine / Q000378:metabolism*; D002460:Cell Line; D004287:Dolichol Monophosphate Mannose / Q000096:biosynthesis; D004288:Dolichol Phosphates / Q000096:biosynthesis*; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D011103:Polyisoprenyl Phosphate Monosaccharides; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis*
D004288:Dolichol Phosphates; D011103:Polyisoprenyl Phosphate Monosaccharides; D011105:Polyisoprenyl Phosphate Sugars; D011106:Polyisoprenyl Phosphates; D004287:Dolichol Monophosphate Mannose; C018439:N-acetylglucosaminylpyrophosphoryldolichol; D005944:Glucosamine; D000117:Acetylglucosamine