Apparent improvement of antisera for radioimmunoassay by treatment with sodium iodide.
The concept that antisera for radioimmunoassay can be improved by treatment with NaI and dialysis is challenged. Some antisera are little affected by NaI. Even when NaI does dissociate endogenous antigenic material from crude antiserum, in the subsequent dialysis NaI is dialysed at a much faster rate than antigen, allowing reassociation to occur. The reported improvement of antisera by the NaI method cannot be attributed to the effect of NaI but can be explained by the dilution which occurs during dialysis.
D000818:Animals; D000936:Antigen-Antibody Complex; D003956:Dialysis; D006041:Goats; D006168:Guinea Pigs; D007106:Immune Sera; D007454:Iodides / Q000378:metabolism / Q000494:pharmacology*; D010455:Peptides / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007454:Iodides; D010455:Peptides