[Studies on the cultivation of isolated murine renal glomeruli (author's transl].
The rate of growth of isolated murine renal glomeruli was studied in vitro. Separation by screening was followed by cultivation in Carrel flacks using Eagle's medium, and the number of utilizable tests was 104. It was possible to cultivate glomeruli for up to 27 days and to observe the growth of cells. These were epithelial capsule cells, endothelial capillary cells and fibroblasts. The existence of mesangial cells not be convincingly demonstrated experimentally. The problems associated with the cultivation of isolated renal glomeruli are discussed with reference to data reported in the literature. Possible uses of this particular model are pointed out in some detail.
D000818:Animals; D002196:Capillaries / Q000166:cytology; D002478:Cells, Cultured; D046508:Culture Techniques; D004727:Endothelium / Q000166:cytology; D004847:Epithelial Cells; D005347:Fibroblasts; D007678:Kidney Glomerulus; D008722:Methods; D051381:Rats; D013997:Time Factors
Untersuchungen zur Kultivation isolierter Rattennierenglomeruli.