A simple method of cell-mediated cytotoxic assay by postlabeling of tritiated thymidine.
The cytotoxic reaction of effector cells on target cells was performed on cover slips which were set in a petri dish so as to be interposed from the dish surface through the bases of the slide glasses. After incubation, the target cells remaining on the cover slips were pulse-labeled with 3H-TdR for 45 min. Subsequently, the cover slips were removed from the bases, and dipped in cold 5% TCA to be freed from non-incorporated isotopes. The residual target cells adhering on the cover slips were counted without being removed. By this assay system, the specific or non-specific cytotoxicity of lymphocytes or macrophages was easily demonstrated with reliability.
D000818:Animals; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D005260:Female; D007553:Isotope Labeling; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051381:Rats; D012513:Sarcoma, Experimental / Q000276:immunology; D013936:Thymidine; D014316:Tritium
D014316:Tritium; D013936:Thymidine