Purification of cDNA complementary to sea urchin histone mRNA.
Complementary DNA (cDNA) was transcribed from a polyadenylated sea urchin histone mRNA preparation isolated by density gradient centrifugation. By hybridization, this cDNA was shown to be extensively contaminated (85% of hybridizable cDNA) with DNA complementary to RNA derived from the large ribosomal subunit. Purification of a mRNA specific cDNA fraction was achieved by hybridization of purified rRNA to cDNA followed by fractionation on hydroxyapatite. After further purification to remove nonhybridizable cDNA our purified cDNA showed only 8% hybirdization to rRNA.
D000818:Animals; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D006657:Histones / Q000096:biosynthesis*; D007700:Kinetics; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D012617:Sea Urchins; D014158:Transcription, Genetic
D006657:Histones; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA