[Penicillin-induced immunhaemolytic anaemia. In vitro studies using separated monocytes (author's transl)].
Under certain conditions human monocytes were able to bind and ingest red cell-antibody complexes in vitro. Using penicillincoated red cells and purified monocytes we investigated sera of patients with penicillin allergy. It was shown that sera containing IgG-antibodies against penicillin induced the binding of penicillin-coated red cells to isolated monocytes provided IgG-antibodies of high titer were present. Inhibition and absorption tests demonstrated the specificity of the reaction in terms of IgG-antibodies and the drug. Monocyte binding was also studied in respect to the cross reactivity of penicillin antibodies and cephalosporins. We concluded that antipenicillin-antibodies of the IgG-class were able to induce an immunphagocytosis in vitro, if the drug was present in the test system. The reaction was dependent on the amount of antibodies of the IgG-class.
D000743:Anemia, Hemolytic / Q000139:chemically induced*; D000906:Antibodies; D001666:Binding Sites, Antibody; D002512:Cephalothin / Q000009:adverse effects*; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D009000:Monocytes; D010406:Penicillins / Q000009:adverse effects*; D012397:Rosette Formation
D000906:Antibodies; D007074:Immunoglobulin G; D010406:Penicillins; D002512:Cephalothin
Immunhämolyse unter Penicillintherapie. In-vitro-Ergebnisse unter Verwendung isolierter Monozyten.