Synthesis and application of new bifunctional reagents.
Two new bifunctional reagents suited for the step-wise cross-linking of cysteine and lysine residues in proteins are described. Application to lactate dehydrogenase yields a cross-link between cysteine-165 and lysine-179, which suggests an alternative mechanism by which the "essential" cysteine reacts. For the mapping of the environment of a known and well defined amino acid the use of semireversible bifunctional reagents is suggested.
D001386:Azides; D055598:Chemical Phenomena; D002621:Chemistry; D003545:Cysteine; D007202:Indicators and Reagents; D008239:Lysine; D046911:Macromolecular Substances; D008301:Maleimides; D008722:Methods; D011506:Proteins
D001386:Azides; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D008301:Maleimides; D011506:Proteins; D008239:Lysine; D003545:Cysteine