[Automated method for determining calcium in biological liquids].
A method of determination of calcium in biological fluids is proposed. It is a continuous flow technic without deproteinisation nor dialysis, using orthocresol phtaleine in alkaline medium. The interference due to magnesium is eliminated by the presence of hydroxy-8-quinoleine. The addition of dimethyl sulfoxide improves the solubility of the reagents and ensures better stability of the media. The correlation between the results obtained by this technic and by atomic absorption spectrophotometry was studied on 160 human sera and 40 urines. The influence of various parameters such as hemolysis, bilirubin, magnesium and phosphates is low or negligeable. The results concerning opalescent, cloudy or lactescent sera may be erroneous by excess. The physiological reference values for serum calcium are drawn up from a Paris student population of both sexes.
D001322:Autoanalysis / Q000379:methods; D002118:Calcium / Q000097:blood*; D006454:Hemoglobins; D006801:Humans; D007202:Indicators and Reagents; D013053:Spectrophotometry
D006454:Hemoglobins; D007202:Indicators and Reagents; D002118:Calcium
Méthode de dosage automatique du calcium dans les liquides biologiques.