Quantitative determination of glycosphingolipids illustrated by using erythrocyte membranes of various mammalian species.
A procedure for the quantative determination of glycosphingolipids is described, involving extraction of total lipids, fractionation on a column, acid cleavage and photometry of a complex formed between sphingosine and methyl orange. The level of glycosphingolipid sphigosine of erythrocyte membranes of a variety of mammals ranges from 59.1 (rat) to 410.1 (pig) nmol/ml packed cells.
D000818:Animals; D002161:Camelids, New World; D002415:Cats; D002417:Cattle; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006028:Glycosphingolipids / Q000097:blood*; D006801:Humans; D008722:Methods; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D006028:Glycosphingolipids