Studies of peroxisomes. VIII. Evidence for framework protein of the cores of rat liver peroxisomes.
The cores of peroxisomes were purified 670 fold from a rat liver homogenate and the protein in the preparation was examined by sodium dodecyl sulfate(SDS)-polyacrylamide gel electrophoresis. Two bands of protein were detected on 10% polyacrylamide gel, and their molecular weights were calculated to be about 32,000 and 27,000. On treatment of the core fraction with alkali, urate oxidase was solubilized and on 10% polyacrylamide gel this fraction gave a single band of protein with an estimated molecular weight of 32,000. These results suggests that the protein component having a molecular weight of 27,000 is the framework protein of the core of rat liver peroxisomes.
D000818:Animals; D008099:Liver / Q000032:analysis* / Q000201:enzymology; D008297:Male; D008830:Microbodies / Q000032:analysis* / Q000201:enzymology / Q000648:ultrastructure; D008970:Molecular Weight; D009940:Organoids / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D014503:Urate Oxidase / Q000032:analysis
D011506:Proteins; D014503:Urate Oxidase