title: High pressure liquid chromatographic separation of C18 and C19 Steroids.
abstract: High Pressure liquid chromatographic procedures for the resolution of mixtures of C18 and C19 steroids of biologic importance are described. On-line monitoring of the absorbance and refractive index of the eluate was found to be suitable for the quantitative measurement of the amounts of eluted steroids. Using a high sensitivity absorbance detector, reliable measurements of as little as 5 ng delta(3)-3 keto steroids were possible.
mesh_terms: D000728:Androgens / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004967:Estrogens / Q000302:isolation & purification*
chemical_list: D000728:Androgens; D004967:Estrogens