An ethidium-induced double helix of poly(dA)-poly(rU).
Equilibrium dialysis, relaxation kinetic, melting, and continuous variation mixing experiments on complexes of poly(dA) and poly(rU) demonstrate that ethidium induces conversion of a 1:1 mixture of these homopolymers (at one molar salt and 19 degrees C) from a three stranded to a two stranded helix. This is the first demonstration of a double helix of poly(dA)-poly(rU) in solution.
D001665:Binding Sites; D004996:Ethidium; D007700:Kinetics; D008433:Mathematics; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D011061:Poly A; D011072:Poly U; D011089:Polydeoxyribonucleotides; D013053:Spectrophotometry; D013696:Temperature; D013816:Thermodynamics
D011089:Polydeoxyribonucleotides; D011061:Poly A; D011072:Poly U; D004996:Ethidium