title: Early effects of phytohemagglutinin on induction of DNA polymerase, thymidine kinase, deoxyribonucleoside triphosphate pools and DNA synthesis in human lymphocytes.
abstract: In phytohemagglutinin stimulated human lymphocytes the time relationship was determined between induction of the parameters mentioned. The results indicate that the induction occurred in a specific sequence. Thus, a simultaneous increase in the activity of DNA polymerase and thymidinekinase occurred after 15 h of incubation with Phytohemagglutinin. Furthermore, this enhancement occurred 2 h before the expansion of the TTP and dCTP pools and 4 h before the expansion of the dATP and dGTP pools. The rate of [3H] deoxyguanosine incorporation into DNA increased simultaneously with the expansion of the TTP and dCTP pools.
mesh_terms: D004247:DNA / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis*; D003854:Deoxyribonucleotides / Q000378:metabolism*; D004790:Enzyme Induction; D006801:Humans; D007700:Kinetics; D037102:Lectins; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D013937:Thymidine Kinase / Q000096:biosynthesis*
chemical_list: D003854:Deoxyribonucleotides; D037102:Lectins; D004247:DNA; D013937:Thymidine Kinase; D004259:DNA-Directed DNA Polymerase