Myo-inositol transport system in Pseudomonas putida.
The kinetic features of the myo-inositol transport system in Pseudomonas putida are reported. The system is sensitive to osmotic shock, is not operative in membrane vesicles, and does not involved substrate phosphorylation. Line-weaver-Burk plots indicate the presence of two different systems, whose Kt are 5 micrometer and 0.43 mM and whose V max are 7.9 and 27 nml/mg per min, respectively. Transport activity of glucose-grown cells is very low. myo-Inositol-grown cells lose the high-affinity system upon osmotic shock; concentrated shock fluid possesses myo-inositol-binding activity. The system is very specific for the myo-configuration of the cyclitol.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001693:Biological Transport, Active; D005947:Glucose / Q000378:metabolism; D007294:Inositol / Q000378:metabolism*; D009997:Osmotic Pressure; D011485:Protein Binding; D011549:Pseudomonas / Q000201:enzymology / Q000378:metabolism*; D013402:Sugar Alcohols / Q000378:metabolism
D001426:Bacterial Proteins; D013402:Sugar Alcohols; D007294:Inositol; D000429:Alcohol Oxidoreductases; D005947:Glucose