Absolute identification of Hb D-Los Angeles (beta121 Glu replaced by Gln).
This paper describes a method which permits the absolute identification of Hb D-Los Angeles without the need for N-terminal or amino acid analysis on peptide beta DT-13. The procedure involves the formation of the 5-oxo-2-pyrrolidinecarboxyl derivative of the N-terminal glutamine residue ot betaDT-13. This method, together with the use of cadmium acetate/ninhydrin staining, allows the identification of Hb D-Los Angeles, and other possible mutations of beta121, by peptide mapping.
D000596:Amino Acids; D002140:California; D055598:Chemical Phenomena; D002621:Chemistry; D005971:Glutamates / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006455:Hemoglobins, Abnormal; D006801:Humans; D010455:Peptides
D000596:Amino Acids; D005971:Glutamates; D006455:Hemoglobins, Abnormal; D010455:Peptides; D005973:Glutamine