Purification of rabbit transcobalamin II by labile ligand affinity chromatography.
Rabbit transcobalamin II has been purified to homogeneity from serum containing 5-30 nmol unsaturated transcobalamin II. The method described consists of labile ligand affinity chromatography and G-200 Sephadex gel filtration. The recovery of pure protein was about 80%.
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D008024:Ligands; D008722:Methods; D011485:Protein Binding; D011817:Rabbits; D014155:Transcobalamins / Q000302:isolation & purification*
D001798:Blood Proteins; D008024:Ligands; D014155:Transcobalamins