An improved assay of gangliosides separated by thin-layer chromatography.
There were no differences in the recoveries of ganglioside sialic acid from silica gel G thin-layer chromatograms when they were sprayed with resorcinol reagent varying in normality between 3 and 10. However, both the temperature at and the time for which the plates were heated after spraying did affect the recoveries, which can reach 100% when the plates are heated for 6 min at 135degreesC.
D002540:Cerebral Cortex / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods; D005732:Gangliosides / Q000032:analysis*; D006801:Humans; D012118:Resorcinols; D013696:Temperature
D005732:Gangliosides; D012118:Resorcinols