title: Effects of androgens on the complexity of poly(A) RNA from rat prostate.
abstract: Poly(A)-containing RNA, isolated from rat ventral prostate, has been analyzed for its base sequence complexity. The kinetics of hybridization of total cellular poly(A)-containing RNA with its complementary DNA (cDNA) from normal and castrated animals are markedly different. RNA from normal animals consists of three abundance classes, about 36% comprises one or two highly abundant RNA sequences, 29% consists of about 24 sequences and the remainder is a scarce class of approximately 8200 sequences. In contrast, the hybridization kinetics of prostatic RNA from castrated animals demonstrate that there is a moderate abundance class of 53 sequences and a scarce class of about 7800 sequences, but that a class of abundant sequences is not present. Using normal prostatic cDNA as a probe, we showed that the abundant sequences were not absent but reduced 10 fold following a 3-day castration period and 100 fold after 100 fold after 7 days. Such heterologous hybridization experiments also suggest that there is significant sequence homology in the RNA sequences present in the prostate irrespective of the hormonal status of the animals. The major effect of testosterone appears to be the regulation of the abundance of specific RNA sequences.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002369:Castration; D008297:Male; D009693:Nucleic Acid Hybridization; D011061:Poly A; D011467:Prostate / Q000502:physiology*; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D011061:Poly A; D013739:Testosterone; D012313:RNA