Selective estimation of aconitine in presence of aconine and benzoylaconine.
A selective and simple colorimetric method is presented for the estimation of aconitine in drugs in the presence of aconine and benzoylaconine. The method is based on the formation of an iron hydroxamate complex through the acetate ester group to which the biological activity is due. The color is measured at 530 nm (5--250 microgram/ml). Under the experimental conditions, neither the benzoyl group of benzoylaconine nor aconine is involved in the process of hydroxylaminolysis.
D000157:Aconitine / Q000032:analysis*; D031841:Aconitum / Q000031:analogs & derivatives* / Q000032:analysis*; D003124:Colorimetry; D008722:Methods; D012996:Solutions / Q000032:analysis
D012996:Solutions; D000157:Aconitine