[Ultraspectrophotometric method of differential determination of DNA and RNA in spermatozoa].
The authors describe a complement of the previously published ultramicrospectro-photometric method for the dosage of nucleic acids. The spermatozoa are scanned by a bundle of U-V. light. It is so possible to obtain the measurement of the U-V. light absorption on a very big number of very small areas. The data are printed very accurately on one histogram. Thereafter spermatozoa are submitted to the action of RNase and DNase and rescanned with U-V. light. The comparison of the histograms allows to estimate the amount of nucleic acids. Further investigations are requested.
D004247:DNA / Q000032:analysis*; D006801:Humans; D008297:Male; D012313:RNA / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013094:Spermatozoa / Q000032:analysis*
D012313:RNA; D004247:DNA
Essai de dosage différentiel par ultramicrospectrophotométrie de l'ADN et de l'ARN dans le spermatozoide (suite).