Fluorescein-labeled estradiol: a probe for anti-estradiol antibody.
A fluorescent  estradiol derivative binds strongly to antiestradiol antibody. The binding, measured by fluorescence polarization, is inhibited by estradiol and by diethylstilbestrol. Tentatively characterized as N-(estradiol-6-iminooxyacetyl) fluorescein amine, the derivative was prepared from estradiol-6-iminooxyacetic acid, dicyclohexylcarbodiimide, and fluorescein amine, and isolated by TLC. It has a fluorescence emission similar to that of fluorescein and an absorption spectrum consistent with a fluorescein: estradiol molar ratio of 1:1.
D000906:Antibodies / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D004958:Estradiol / Q000276:immunology*; D005452:Fluoresceins; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D000906:Antibodies; D005452:Fluoresceins; D004958:Estradiol