Chromatin very small angle neutron scattering: further evidence for a 30 nm diameter super coil in dilute solutions.
Intact chromatin, chromatin minus histone H1, and nuclease digestion fragments have been studied by very small angle neutron scattering. The results are not consistent with a straight chain of nucleosomes and require the presence of a higher order coiling in monovalent salt solutions. The data are interpretable by a structure having a cross section radius of gryration of 8.5 +/- 1 nm, which suggests an outer diameter for a coil of nucleosomes of 27 +/- 3 nm.
D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA; D003851:Deoxyribonucleases; D006657:Histones; D009502:Neutrons; D009690:Nucleic Acid Conformation; D051381:Rats; D012542:Scattering, Radiation
D002843:Chromatin; D006657:Histones; D004247:DNA; D003851:Deoxyribonucleases