Bloom's syndrome: DNA replication in cultured fibroblasts and lymphocytes.
Analysis of DNA fiber autoradiograms from Bloom's syndrome skin fibroblasts and blood lymphocytes shows a retarded rate of replication fork movement compared to normal adult controls. Other measurements from the autoradiograms--replication unit length, incidence of bidirectional replication, and degree of initiation synchrony--are normal in Bloom's syndrome cells. These results suggest that a slow rate of fork movement is a specific manifestation of defective DNA synthesis in all Bloom's syndrome cells.
D000015:Abnormalities, Multiple / Q000473:pathology*; D002478:Cells, Cultured; D004261:DNA Replication; D004392:Dwarfism / Q000473:pathology; D005148:Facial Dermatoses / Q000473:pathology*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D013577:Syndrome; D013684:Telangiectasis / Q000473:pathology*