Polynucleotides. XLV Synthesis and properties of poly(2'-azido-2'-deoxyinosinic acid).
Poly (2'-azido-2'-deoxyinosinic acid), [poly (Iz)], was synthesized from 2'-azido-2'-deoxyinosine diphosphate by the action of polynucleotide phosphorylase. Poly (Iz) has UV absorption properties similar to poly (I) and hypochromicity of 11% at 0.15M Na+ and neutrality. In solutions of high Na+ ion concentration, poly (Iz) forms a multi-stranded complex and its Tm at 1.0M Na+ ion concentration was 43 degrees. Upon mixing with poly (C), poly (Iz) forms a 1:1 complex having a Tm lower than that of poly (I)-poly (C) complex in the same conditions. The effect of substitution at the 2'-position of the poly (I) strand was discussed in relation to the interferon-inducing activity.
D001386:Azides; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D007292:Inosine Nucleotides; D007700:Kinetics; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D011066:Poly C; D011089:Polydeoxyribonucleotides / Q000096:biosynthesis; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D001386:Azides; D007292:Inosine Nucleotides; D046911:Macromolecular Substances; D011089:Polydeoxyribonucleotides; D011066:Poly C; D011117:Polyribonucleotide Nucleotidyltransferase