Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants.
1. The partial purification of two lithocholic acid-binding proteins from liver 100 000g supernatants is described. 2. Gel-filtration, (NH4)2SO4 fractionation, Ca3(PO4)2 fractionation and ion-exchange chromatography were used. 3. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. 4. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.
D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D002352:Carrier Proteins / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors; D008095:Lithocholic Acid; D008099:Liver / Q000032:analysis*; D008297:Male; D051381:Rats
D001647:Bile Acids and Salts; D002352:Carrier Proteins; D008095:Lithocholic Acid; D005982:Glutathione Transferase