Aflatoxin in corn: evaluation of filter fluorometer reading of minicolumns.
A minicolumn method using a filter fluorometer was compared with thin layer chromatography for determining aflatoxin in corn. The minicolumn-fluorometer method identified many more samples as aflatoxin-positive than the AOAC CB method. Some corn samples contain a blue-fluorescing substance that interferes with minicolumn chromatography of aflatoxins. Aflatoxin levels measured by the minicolumn-fluorometer method did not correspond to those obtained by the CB method.
D000348:Aflatoxins / Q000032:analysis*; D001322:Autoanalysis; D005069:Evaluation Studies as Topic; D005470:Fluorometry / Q000295:instrumentation*; D008832:Microchemistry; D003313:Zea mays / Q000032:analysis*
D000348:Aflatoxins