Effect of berenil on growth, mitochondrial DNA and respiration of Leishmania tarentolae promastigotes.
Incubation of Leishmania tarentolae promastigotes in 1.0 microgram/ml Berenil for 96 hr resulted in approximately 33% inhibition of cell growth and approximately 42.5% dyskinetoplasty in the cell population. The buoyant density of kinetoplast DNA (kDNA), p = 1.703 g/ml, remained unchanged after 96-hr exposure to the drug. Endogenous respiration as well as proline- and glucose-induced respiration dropped markedly after 36-hr exposure to Berenil. This drop occurred approximately 12 hr before the onset of dyskinetoplasty, a result which suggests that this drug adversely affects mitochondrial respiratory activity of the promastigotes.
D000578:Amidines / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004272:DNA, Mitochondrial / Q000378:metabolism*; D004133:Diminazene / Q000494:pharmacology*; D005407:Flagella / Q000648:ultrastructure; D007891:Leishmania / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008928:Mitochondria / Q000648:ultrastructure; D010101:Oxygen Consumption / Q000187:drug effects*
D000578:Amidines; D004272:DNA, Mitochondrial; D004133:Diminazene