DNA-fluorometry of mammalian sperm.
The Dna-content of sperm and testicular cells was measured by pulse-cytophotometry with high resolution. From flat sperm symmetric and narrow fluorescene distributions were obtained. Enzymatic treatment with papain or pronase and staining with an ethidiumbromide-mithramycin dye solution generate stoichiometric DNA-staining including that of mature sperm with a coefficient of variation below 2%.
D000818:Animals; D004247:DNA / Q000032:analysis*; D006801:Humans; D008297:Male; D013045:Species Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013094:Spermatozoa / Q000032:analysis*; D013737:Testis / Q000032:analysis
D004247:DNA