title: Adsorption of polymorphonuclear leukocyte lysosomal enzymes to monosodium urate crystals.
abstract: Monosodium urate (MSU) crystals induced prompt release of lysozyme, and slower release of beta-glucuronidase, alpha-mannosidase, and lactic dehydrogenase (LDH) from polymorphonuclear leukocytes. At increasing crystal concentrations, an increasing delay in the apparent onset of beta-glucuronidase release was detected which appears to be due to selective absorption of enzyme activities to the MSU crystals: beta-glucuronidase greater than LDH greater than alpha-mannosidase greater than lysozyme. Lysosomal enzyme adsorption by MSU crystals may then contribute to experimental error or may modulate gouty inflammation.
mesh_terms: D000327:Adsorption; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology; D008361:Mannosidases / Q000378:metabolism*; D009113:Muramidase / Q000378:metabolism*; D009504:Neutrophils / Q000201:enzymology*; D014527:Uric Acid / Q000378:metabolism*
chemical_list: D014527:Uric Acid; D007770:L-Lactate Dehydrogenase; D008361:Mannosidases; D009113:Muramidase; D005966:Glucuronidase