Production of antisera against contraceptive steroids.
A four step synthesis of 6-(0-carboxymethyl) oximinoethynylestradiol is reported. This compound, 6-(0-carboxymethyl) oximinomestranol, the 3-(0-carboxymethyl) oximes of norethindrone and norgestrel and the 3-hemisuccinate of ethynylestradiol were synthesized and conjugated with bovine serum albumin. Rabbits were immunized at 3 dose levels of haptene (20, 66 and 200 nmoles) and eight weeks later with a booster containing 66 nmoles of haptene. The antibody titer and association constant of responding rabbits was nearly independent of dose although most antibody production occurred after the booster injection. Antibodies to mestranol crossreacted more than 100 percent with ethynylestradiol and to a small extent with norethindrone and norgestrel.
D000818:Animals; D000917:Antibody Formation; D003276:Contraceptives, Oral / Q000276:immunology*; D003280:Contraceptives, Oral, Synthetic / Q000138:chemical synthesis / Q000276:immunology*; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D045166:Estradiol Congeners / Q000276:immunology; D006241:Haptens; D045167:Progesterone Congeners / Q000276:immunology; D011817:Rabbits
D003276:Contraceptives, Oral; D003280:Contraceptives, Oral, Synthetic; D045166:Estradiol Congeners; D006241:Haptens; D045167:Progesterone Congeners