[Increase of cholesterol hydroxylation in rat liver as a result of induction of microsomal enzymes].
A possibility to increase 7alpha-hydroxylation of 4-14C-cholesterol was studied in vitro, using microsomal enzymes from rat liver tissue, under effect of such inductors of these enzymes as 5-ethyl-5-phenyl barbituric acid (phenobarbital), pregnenolone-16alpha-carbonitrile, 16-dehydropregnenolone, 3-acetate-16alpha-isothiocyanogen pregnenolone. These inductors were administered per os within 5 days as an aqueous suspension, stabilized with Tween 80 at a dose 50 mg/kg daily; ethyl ester alpha-(p-chlorophenhydroxy)-isobutyric acid (klofibrate "Miskleron*) was administered at a dose of 150 mg/kg daily within 5 days. All the inductors studied increased the activity of cholesterol 7alpha-hydroxylase: pregnenolone-16alpha-carbonitrile--by 50%, 16-dehydropregnenolone--by 80%, 3-acetate-16alpha-isthiocyanogen pregnenolone--by 110% and phenobarbital--by 200%. Klofibrate did not affect the intensity of the cholesterol hydroxylation.
D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D002790:Cholesterol 7-alpha-Hydroxylase / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology* / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology; D011284:Pregnenolone / Q000031:analogs & derivatives / Q000494:pharmacology; D011285:Pregnenolone Carbonitrile / Q000494:pharmacology; D051381:Rats; D013250:Steroid Hydroxylases / Q000096:biosynthesis*
D011285:Pregnenolone Carbonitrile; D011284:Pregnenolone; D002784:Cholesterol; D013250:Steroid Hydroxylases; D002790:Cholesterol 7-alpha-Hydroxylase; D010634:Phenobarbital
Usilenie gidroksilirovaniia kholesterina v pecheni krys v rezul'tate induktsii mikrosomal'nykh fermentov.