Internal proteins of influenza virus: 35S-methionine peptide maps as genetic markers.
Methods are described for the preparation in vivo of 35S-methionine-labelled influenza viruses, the purifiction of the nucleoprotein (NP) and matrix (M) proteins and the separation of peptides obtained by protease digestion by two-dimensional thin-layer chromatography. The maps of the M proteins of A/Okuda/57(H2N2) and A/Finland/4/74(H3N2) were very similar overall but differed in three peptides. Hence they could be clearly distinguished. Maps of the NP proteins of the same strains showed a greater number of differences. A recombinant strain having the haemagglutinin and neuraminidase of the A/Finland/4/74 parent and the virulence of the A/Okuda/57 parent was shown to have the M and P proteins of A/okuda/57.
D002874:Chromosome Mapping; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics; D009980:Influenza A virus / Q000235:genetics*; D008715:Methionine; D009439:Neuraminidase / Q000235:genetics; D009698:Nucleoproteins / Q000235:genetics*; D010455:Peptides / Q000235:genetics*; D011995:Recombination, Genetic; D013462:Sulfur Radioisotopes; D014764:Viral Proteins / Q000235:genetics*; D014771:Virion / Q000235:genetics; D014774:Virulence
D006389:Hemagglutinins, Viral; D009698:Nucleoproteins; D010455:Peptides; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D008715:Methionine; D009439:Neuraminidase