Release of thiamine and formation of a methylthiamine-like substance in the phrenic nerve-diaphragm preparation of the rat.
An increased formation of a radioactive methylthiamine-like substance (MTLS) in the end plate region of the rat diaphragm muscle and a release of radioactivity chromatographed as thiamine into the bath medium after a subcutaneous injection of 35S-thiamine were found after stimulation of the phrenic nerve. There was also an increased formation of the radioactive methylthiamine-like substance in the denervated diaphragm preparation incubated with radioactive acetylcholine (ACh) in comparison with the innervated diaphragm.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D003964:Diaphragm; D004558:Electric Stimulation; D006168:Guinea Pigs; D008297:Male; D009045:Motor Endplate / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects; D009121:Muscle Denervation; D009132:Muscles / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000378:metabolism*; D009538:Nicotine / Q000494:pharmacology; D010525:Peripheral Nerves / Q000378:metabolism*; D010791:Phrenic Nerve; D051381:Rats; D013831:Thiamine / Q000031:analogs & derivatives* / Q000378:metabolism*
D009538:Nicotine; D000109:Acetylcholine; D013831:Thiamine