title: Bisulfite reductase of Desulfovibrio vulgaris: explanation for product formation.
abstract: Bisulfite reductase, purified from Desulfovibrio vulgaris, was coupled with the pyruvate phosphoroclastic reaction. Moderate to low reducing conditions resulted in the formation of trithionate; however, when the concentration of reductant was high, a mixture of trithionate and thiosulfate was formed. Sulfide was also a detectable product, but only when the concentration of bisulfite was low. Flavodoxin mediated native coupling between bisulfite reductase and the phosphoroclastic reaction. A model for bisulfite reductase activity is proposed.
mesh_terms: D003901:Desulfovibrio / Q000201:enzymology* / Q000378:metabolism; D004579:Electron Transport; D005418:Flavodoxin / Q000378:metabolism; D008954:Models, Biological; D010088:Oxidoreductases / Q000378:metabolism*; D010269:Paraquat / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D013440:Sulfides / Q000378:metabolism; D013447:Sulfites / Q000378:metabolism; D013885:Thiosulfates / Q000378:metabolism*
chemical_list: D005418:Flavodoxin; D011773:Pyruvates; D013440:Sulfides; D013447:Sulfites; D013885:Thiosulfates; D010088:Oxidoreductases; D010269:Paraquat