Glycosyl moiety of the lima bean lectin.
Pronase digestion of the lima bean lectin (Phaseolus lunatus (alpha-D-GalNAc greater than alpha-D-Gal)) afforded a glycopeptide of molecular weight 1380. Amino acid and carbohydrate analysis showed the glycopeptide to contain fucose, mannose, glucosamine, and aspartic acid in the molar ratios, 0.8:4.4:1.9:1.0. Methylation analysis, Smith degradation, and sequential degradation by specific glycosidases permit the formulation of a tentative structure:.
D001224:Aspartic Acid / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D007887:Fabaceae; D005643:Fucose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006020:Glycopeptides / Q000302:isolation & purification; D006026:Glycoside Hydrolases; D037102:Lectins; D008358:Mannose / Q000032:analysis; D037121:Plant Lectins; D010946:Plants, Medicinal; D011402:Pronase
D006020:Glycopeptides; D037102:Lectins; D037121:Plant Lectins; D005643:Fucose; D001224:Aspartic Acid; D006026:Glycoside Hydrolases; D011402:Pronase; D005944:Glucosamine; D008358:Mannose