Cytotoxicity inhibition assay: cryopreservation and standardization: brief communication.
The cytotoxicity inhibition assay is an extremely useful tool for examination of the specificity of cell-mediated cytotoxicity. With this demonstration of cryopreservation of the attacker, target, and inhibitor cells, a powerful new tool is available for the standardization of this assay to better examine the specificity of cytotoxicity assays. Not only can cryopreservation be used to standardize the assay within a single laboratory, but also reagents may now be exchanged between laboratories to better understand the differences in cell-mediated cytotoxicity as observed by different investigators.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D005615:Freezing; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology*; D011309:Preservation, Biological; D051381:Rats