Determination of paraoxon by combined bovine liver esterase inhibition and gas-liquid chromatography.
A combined gas-liquid chromatography and enzyme procedure is described for the determination of organophosphorus compounds that show anticarboxylesterase activity in vitro. The method is based on the enzyme hydrolysis of 1-naphthyl acetate and on the determination of both the release 1-naphthol and the unhydrolyzed 1-naphthyl acetate by gas-liquid chromatography. With bovine liver as the source of enzyme the detection limit for paraoxon was 13 ppb.
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas / Q000379:methods; D004950:Esterases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008832:Microchemistry; D010261:Paraoxon / Q000032:analysis* / Q000378:metabolism
D004950:Esterases; D010261:Paraoxon