Mass fragmentographic determination of lofepramine and its metabolites in human plasma and urine using deuterated internal standards.
A sensitive and specific method for the determination of lofepramine and its matabolites, desipramine and 2-hydroxydesipramine, in human plasma and urine is described. Lofepramine, desipramine and 2-hydroxydesipramine were derivatized to ethyl p-chlorobenzoate, the bis(heptafluorobutyryl) derivative and teh N,O-bis(trifluoroacetyl) derivative, respectively, and then analysed by gas chromatogrphy--mass fragmentography. Corresponding deuterated compounds were used as internal standards. Determination was possible at levels as low as 2 ng/ml for lofepramine and desipramine and 20 ng/ml for 2-hydroxydesipramine.
D000929:Antidepressive Agents, Tricyclic / Q000032:analysis* / Q000097:blood / Q000652:urine; D003891:Desipramine / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D003984:Dibenzazepines / Q000032:analysis* / Q000097:blood / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans
D000929:Antidepressive Agents, Tricyclic; D003984:Dibenzazepines; D003891:Desipramine