Glomerular metabolism in protein-load proteinuria.
Rats were injected with various amounts of bovine albumin (0.5, 1.0 and 1.75 g/24h), inducing thereby proteinuria ranging from 100 to 400 mg/24h. The glomerular oxygen uptake, dry weight and glucose-6-phosphate dehydrogenase (G-6-PHD) activity were measured on the 4th day of proteinuria and in a group of control animals. Oxygen uptake increased of +60%, expressed per glomerulus and of +25% when expressed per milligram dry weight and this increase was not different between the 3 groups of rats. Glomerular dry weight increased significantly in the 3 series. There was an highly significant relationship between glomerular dry weight and oxygen uptake, combining the 3 series together. G-6-PDH increased as expected from previous experiments and this increase was more marked for the more marked proteinuria. The relationship between G-6-PDH and QO2 was of borderline statistical significance (p=0.05). The glomerular hypertrophy, oxidative hyperactivity and increase in G-6-PDH activity are probably related to transcellular transport of protein.
D000418:Albumins; D000818:Animals; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007678:Kidney Glomerulus / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D009929:Organ Size; D010101:Oxygen Consumption; D011507:Proteinuria / Q000201:enzymology / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats
D000418:Albumins; D005954:Glucosephosphate Dehydrogenase