title: Cellular and humoral immunity to leukemia cells in BCG-induced growth control of a murine leukemia.
abstract: The antitumor effects of weekly iv injections of 1.0 mg BCG and/or sc injections of 10(7) irradiated leukemia cells were studied in an isogeneic, transplantable lymphoid leukemia in the C57BL/6 mouse. The injections were started at day 1 after ip inoculation of 10(5) leukemia cells. BCG prolonged the survival time of most animals and cured 22%. BCG plus irradiated cells cured only about 10% of the mice, and irradiated cells alone had no curative effect. Individual tumor-bearing mice in the various experimental groups were examined with respect to ascites tumor cell number; complement-dependent cytotoxic antibodies in sera; direct and antibody-dependent cytotoxicity to tumor cells of lymphoid cells from peritoneal fluid, the spleen, and peripheral lymph nodes; and the cytology of ascites, the spleen, and lymph nodes. Only the antibody-dependent lymphocyte-mediated cytotoxicity (ADLMC) was correlated with the ascites tumor cell number, since the ADLMC was high only in mice with a tumor cell number less than that of the controls. Furthermore, since mice with a low tumor cell number had predominantly only lymphocytes as the nonmalignant cell type in their peritoneal fluid, ADLMC may have had an important role in BCG-induced control of tumor growth.
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm / Q000008:administration & dosage; D001202:Ascitic Fluid / Q000166:cytology; D001500:BCG Vaccine / Q000627:therapeutic use*; D002455:Cell Division; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D005260:Female; D007109:Immunity; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D007700:Kinetics; D007942:Leukemia, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D013154:Spleen / Q000276:immunology; D014185:Transplantation, Isogeneic
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D001500:BCG Vaccine; D003165:Complement System Proteins