Evaluation of crew skin flora under conditions of a full quarantine lunar-exploration mission.
Crew-members of the Apollo 14 lunar exploration mission underwent a pre-flight seclusion designed to stabilize their health by freeing them from exposure to potentially infectious agents. After the flight, the crew-members were quarantined to protect the biosphere from possible lunar contamination. These isolations, along with the complete isolation of the spaceflight itself, provided the opportunity for a skin flora survey which included the sampling of seven sites at five different times. Quantification and identification of all aerobic and anaerobic bacteria from each site were performed. The results indicated that the pre-flight quarantine measures resulted in a decrease in total numbers of isolates as well as a decrease in the anaerobes. This was followed by a continued decrease throughout the flight with a return to the pre-flight norm within 16 days after the flight. The quantitative load of aerobic bacteria increased during the flight, due largely to an increase in coryneforms and micrococcaceae. The quantitative load of anaerobic bacteria decreased before and during the flight. No instance of microbial shock or intercrew transfer of micro-organisms was demonstrated. These findings indicate that alterations in the skin flora do not pose any unusual problem during short duration space flights. Further, there are no indications that problems will arise on longer missions.
D001419:Bacteria / Q000302:isolation & purification*; D005118:Extraterrestrial Environment; D006088:Gram-Negative Aerobic Bacteria / Q000302:isolation & purification; D006089:Gram-Negative Anaerobic Bacteria / Q000302:isolation & purification; D006801:Humans; D008297:Male; D011790:Quarantine; D012867:Skin / Q000382:microbiology*; D013026:Space Flight