A newly designed decantation for purified synaptic vesicle.
A new decanting device of fine stainless steel needles has been developed, which can remove a layer from a gradient with little contamination from the adjacent upper or lower layers. This new apparatus can be used for removal of a very narrow band from a density gradient and has been successfully used in the fractionation of synaptic vesicles from brain homogenates.
D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000295:instrumentation / Q000379:methods; D009445:Neuroanatomy / Q000379:methods*; D013572:Synaptic Vesicles / Q000166:cytology*