High-pressure liquid chromatographic analysis of drugs in biological fluids. IV. Determination of clofibrinic acid.
A rapid, sensitive and specific high-pressure liquid chromatographic method is described for the quantitative analysis of clofibrinic acid in plasma, saliva and urine. In contrast to previously reported gas-liquid chromatographic methods, which require derivatization of clofibrinic acid before chromatography, the present method involves a simple two-step extraction procedure and chromatographic determination of the underivatized clofibrinic acid. Concentrations between 1.0 and 25.0 microgram per sample can be measured with a coefficient of variation from 1 to 6%.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002994:Clofibrate / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D006801:Humans; D008297:Male; D012463:Saliva / Q000032:analysis; D013997:Time Factors
D002994:Clofibrate