title: [Polyacrylamide gel electrophoresis of Theragra chalcogramma and bovine opsins in the presence of sodium dodecyl sulphate].
abstract: Fish and bovine opsins were found generally in monomeric forms with slight content of dimers at electrophoresis in 1% sodium dodecyl sulphate--0.005 M sodium phosphate buffer (system I). Opsins have a high tendency to oligomerization and formation of trimers and higher oligomers in 1+5 M urea (system II). The molecular weight of nonomers and dimers of opsins in system I calculated according to the standard proteins, increased with a rise of acrylamide concentration. The authors believe that in both systems the hydrophobic interactions that not the reactions of intermolecular S-S bonds formation are supposed to be on the basis of opsins oligomerization.
mesh_terms: D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D046911:Macromolecular Substances; D008970:Molecular Weight; D012168:Retinal Pigments / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity
chemical_list: D046911:Macromolecular Substances; D012168:Retinal Pigments; D012967:Sodium Dodecyl Sulfate
vernacular_title: Elektroforez opsinov ryby (mintaia) i byka v poliakrilamidnom gele v prisutstvii dodetsilsul'fata natriia.