Determination (by methylation analysis) of the substitution pattern of 2-amino-2-deoxyhexitols obtained from O-glycosylic carbohydrate units of glycoproteins.
The derivatives obtained by permethylation of unsubstituted 2-amino-2-deoxy-hexitols and of these compounds monosubstituted at C-3, C-4, or C-6, and disubstituted at C-3 and C-6, have been analysed by g.l.c.-m.s. Each derivative can be identified on the basis of retention time and mass spectrum. In methylation analysis, methanolysis gave one derivative of each hexitol, whereas a mixture of products was formed when degradation was effected by acetolysis followed by hydrolysis. An application in the analysis of amino-sugar linkages in alkali-labile O-glycosylic oligosaccharides from rat-brain glycoproteins is described.
D000606:Amino Sugars; D000818:Animals; D001923:Brain Chemistry; D003837:Deoxy Sugars; D006020:Glycopeptides; D006023:Glycoproteins; D006027:Glycosides; D008745:Methylation; D009844:Oligosaccharides; D051381:Rats
D000606:Amino Sugars; D003837:Deoxy Sugars; D006020:Glycopeptides; D006023:Glycoproteins; D006027:Glycosides; D009844:Oligosaccharides