Rapid enzymic micromethod for the quantitative determination of L(+)alanine in blood and urine.
A micromethod for the quantitative determination of L-alanine in blood and urine is described. No interference of D-alanine, D-cysteine, L-proline, L-glutamine, L-valine, L-histidine or glycine in concentrations up to 5 mmol/l was found. Using this method fasting blood levels of L-alanine in children were determined. Defects in gluconeogenesis can be easily established by measuring with this method the in vivo L-alanine metabolism in an oral, or intravenous L-alanine loading test.
D000409:Alanine / Q000032:analysis* / Q000097:blood / Q000652:urine; D002648:Child; D006801:Humans; D008722:Methods; D008832:Microchemistry; D010088:Oxidoreductases; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D010088:Oxidoreductases; D000409:Alanine