[Electron microscopic study of protoplasts of mesophyll tobacco. I. Ultrastructural and cytochemical study of tobacco protoplasts at early stages of cultivation].
Ultrastructural changes in tobacco mesophyll protoplasts during the first three days of cultvation were studied. Localization of catalase and peroxidase activities in the freshly isolated protoplasts and 2 days after their cultivation was detected by the cytochemical 3,3'-diaminobenzidine method. A conclusion is drawn that the observed changes in the fine structure and localization of the enzymic activity are due to the following processes: reparation of disturbances caused by the isolation procedure, regeneration of the cell wall and rapid dedifferentiation of the initial parenchymatic leaf cells.
D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D006651:Histocytochemistry; D008854:Microscopy, Electron; D010544:Peroxidases / Q000378:metabolism; D010947:Plants, Toxic; D011523:Protoplasts / Q000201:enzymology / Q000648:ultrastructure*; D014026:Nicotiana / Q000201:enzymology / Q000648:ultrastructure*
D010544:Peroxidases; D002374:Catalase
Elektronnomikroskopicheskoe izuchenie protoplastov mezofilla tabaka. Soobshchenie I. Ultrastrukturnoe i tsitokhimicheskoe issledovanie protoplastov tabaka na rannikh stadiiakh kul'tivirovaniia.