Single-step separation of major and rare ribonucleosides and deoxyribonucleosides by high-performance liquid cation-exchange chromatography for the determination of the purity of nucleic acid preparations.
A method is described for the separation of 13 major and minor ribo- and deoxyribonucleosides in a single chromatographic run using high-performance liquid chromatography on strongly acidic cation-exchange columns. The method proved useful for the routine determination of small amounts of ribonucleic acid impurities in deoxyribonucleic acid preparations and vice versa. About 3% or even less of nucleic acid contamination in a given sample can be easily detected and quantitatively determined under the conditions used.
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000032:analysis; D003853:Deoxyribonucleosides / Q000302:isolation & purification*; D004340:Drug Contamination; D008722:Methods; D012313:RNA / Q000032:analysis; D012263:Ribonucleosides / Q000302:isolation & purification*
D003853:Deoxyribonucleosides; D012263:Ribonucleosides; D012313:RNA; D004247:DNA