Fluorimetric assay of cephradine, cephalexin and cephaloglycin.
1 A simple, rapid and reproducible fluorimetric assay for cephradine, cephalexin and cephaloglycin is described. 2 The method involves addition of formaldehyde which catalyses the formation of fluorescent derivatives. 3 The structural similarities between the side chains of these antibiotics and their identical excitation and emission spectra suggest that they may be forming similar fluorescent derivatives.
D002506:Cephalexin / Q000032:analysis* / Q000097:blood; D002507:Cephaloglycin / Q000032:analysis* / Q000097:blood; D002511:Cephalosporins / Q000032:analysis*; D002515:Cephradine / Q000032:analysis* / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D008722:Methods; D013050:Spectrometry, Fluorescence
D002511:Cephalosporins; D002515:Cephradine; D002507:Cephaloglycin; D002506:Cephalexin