Determination of hydrazine metabolites of isoniazid in human urine by gas chromatography.
A method is described for the determination of isoniazid, acetylisoniazid, acetylhydrazine, diacetylhydrazine and hydrazine in urine. Isoniazid, acetylhydrazine and hydrazine are reacted in aqueous solution  with p-chlorobenzaldehyde to form hydrazones. Following the addition of appropriate internal standards, these hydrazones are then extracted into an organic solvent and determined by gas chromatography using a nitrogen-sensitive detector. Acetylisoniazid and diacetylhydrazine are determined similarly after hydrolysis to isoniazid and acetylhydrazine, respectively.
D000107:Acetylation; D002849:Chromatography, Gas; D006801:Humans; D006834:Hydrazines / Q000138:chemical synthesis / Q000652:urine*; D007538:Isoniazid / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000652:urine*; D008722:Methods
D006834:Hydrazines; D007538:Isoniazid