Partial purification of rat alpha-lactalbumin mRNA.
Alpha-lactalbumin messenger RNA was partially purified from RNA extracted from 3-5 day lactating rat mammary glands on a poly(U)-sepharose column followed by sucrose gradient centrifugation. Apha-Lactalbumin mRNA activity was assayed in wheat germ cell-free translational system by immunoprecipitation of the in vitro synthesized protein using specific antiserum prepared against purified rat alpha-lactalbumin. In the purified mRNA preparation alpha-lactalbumin mRNA activity comprised approximately 85% of the total mRNA activity.
D000818:Animals; D002474:Cell-Free System; D005260:Female; D007768:Lactalbumin / Q000096:biosynthesis*; D007774:Lactation; D008321:Mammary Glands, Animal / Q000032:analysis*; D011247:Pregnancy; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D014908:Triticum
D012333:RNA, Messenger; D007768:Lactalbumin