Effects of prostaglandins E2 and F2alpha on lecithin biosynthesis by cultured lung cells.
Transformed cells from human lung carcinoma (Line A549), resembling type II pneumocytes, were cultured in monolayer at 37 degrees C and incubated for five hours with 3H-choline and 14C-palmitate in the presence of various concentrations of prostaglandins (PGS) E2 and F2alpha. In the control (no PG) the level of % palmitate incorporation was 13.5 x as high as that of choline, after taking isotope dilution into account. Between the concentrations studied, 0.1 and 10 muM, both prostaglandins stimulated markedly the incorporation of both precursors, though choline up to 3 x better than palmitate. This was indicated by a change in the palmitate/choline incorporation ratio from 13.5 to as low as 4.2. At the lowest PG concentration, 0.1 muM, PGE2 was much more effective than PGF2alpha in stimulating the incorporation of both precursors.
D002460:Cell Line; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D003470:Culture Media; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism; D010168:Palmitates / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology*
D003470:Culture Media; D010168:Palmitates; D010713:Phosphatidylcholines; D011458:Prostaglandins E; D011460:Prostaglandins F; D002794:Choline