Identification of the mouse chromosomes by microdensitometry.
Densitometric analysis has been performed on mouse chromosomes from "in vivo" preparations stained with Giemsa after trypsinisation. The characteristic curves obtained by analysing the same chromosome after different durations of trypsinisation suggest that a trypsinisation step is not always necessary to get an analysable banding pattern. The best results are obtained when the chromatids are long and closely appressed and by analysing the positive photomicrographs.
D000818:Animals; D001399:Azure Stains; D002875:Chromosomes; D003720:Densitometry; D007621:Karyotyping / Q000379:methods*; D051379:Mice; D008806:Mice, Inbred AKR; D008938:Mitosis; D014357:Trypsin
D001399:Azure Stains; D014357:Trypsin