[Cytophotometric correlation of changes in the RNA and protein concentrations of perineuronal oligodendrogliocytes and ependymal cells of the spinal cord under different experimental conditions].
By means of ultraviolet and visible cytospectrophotometry, RNA and total protein content per cell was determined in perineuronal oligodendrogliocytes of spinal cord anterior horns and in ependyma cells of spinal cord central canal in Wistar rats under various experimental conditions. It was only in one experimental series that the cell kinds compared were characterized by a similar metabolic response: daily adrenaline injections for two weeks resulted in RNA accumulation both in the oligodendroglia and in the ependyma: besides, in both the kinds of spinal cord cells, no changes in RNA amount was found due to acute hypoxic hypoxia and to 6-mercaptopurine administration. In all the other experimental series (aurantin adminstraion: adrenalectomy and hydrocortison treatment; posthypoxic reparation), changes in RNA content markedly differed in the oligodendroglia and in the ependyma. The data are presented concerning the changes in protein content in the cytoplasm of spinal cord ependyma cells under the experimental conditions applied. Importance of topochemical analysis of nervous tissue structures by means of quantitative cytochemical methods is outlined.
D000315:Adrenalectomy; D000818:Animals; D004805:Ependyma / Q000201:enzymology*; D004837:Epinephrine / Q000494:pharmacology; D006651:Histocytochemistry / Q000379:methods; D006854:Hydrocortisone / Q000494:pharmacology; D000860:Hypoxia / Q000201:enzymology; D008297:Male; D015122:Mercaptopurine / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000201:enzymology*; D009836:Oligodendroglia / Q000201:enzymology*; D010783:Photometry / Q000379:methods; D012313:RNA / Q000378:metabolism; D051381:Rats; D013116:Spinal Cord / Q000201:enzymology*
D009419:Nerve Tissue Proteins; D012313:RNA; D015122:Mercaptopurine; D006854:Hydrocortisone; D004837:Epinephrine
Tsitofotometricheskoe sopostavlenie izmeneniĭ soderzhaniia RNK i belka v perineĭronal'nykh oligodendrogliotsitakh i kletkakh épendimy spinnogo mozga pri razlichnykh éksperimental'nykh vozdeĭstviiakh