[Experimental study of the synthesis of lysosomal enzymes by the synovial membrane: acid phosphatases].
In a system consisting of synovial samples taken surgically and maintained in culture, the authors studied the regulation of enzyme synthesis by taking acid phosphatase as the test enzyme. By means of double labelling and the use of protein synthesis inhibitors, they were able to demonstrate that this synthesis was stimulated by the addition of rheumatoid factor to the culture medium and that it depended on the transcription activity of the genome and on the translation of the information molecules.
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D002850:Chromatography, Gel; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D008247:Lysosomes / Q000201:enzymology*; D010003:Osteoarthritis / Q000201:enzymology; D014176:Protein Biosynthesis; D013583:Synovial Membrane / Q000201:enzymology*
D002250:Carbon Radioisotopes; D007527:Isoenzymes; D003609:Dactinomycin; D003513:Cycloheximide; D000135:Acid Phosphatase
Etude expérimentale de la synthèse des enzymes lysosomiales par la synoviale: phosphatases acides