Absence of messenger RNA and gene DNA for beta-globin chains in hereditary persistence of fetal hemoglobin.
The relative amounts of alpha-amd beta-globin mRNA and globin gene DNA were measured in reticulocyte RNA and lymphocyte DNA of an individual with homozygous hereditary persistence of fetal hemoglobin whose red blood cells contain 100% fetal hemoglobin (hb F: alpha2gamma2.) Molecular hybridization assays used as probes full-length DNA copies of human alpha- and beta-globin messenger RNA. The results of these hybridization assays demonstrated the expected amounts of alpha-globin mRNA and gene DNA, but absence of beta-globin mRNA and absence of beta-globin gene DNA. In the individual studied, hereditary persistence of fetal hemoglobin is associated with total deletion of the beta-globin structural gene.
D002648:Child; D004247:DNA / Q000032:analysis*; D005260:Female; D005319:Fetal Hemoglobin; D005796:Genes; D005914:Globins / Q000096:biosynthesis*; D006453:Hemoglobinopathies / Q000097:blood / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D012156:Reticulocytes / Q000032:analysis
D012333:RNA, Messenger; D005914:Globins; D004247:DNA; D005319:Fetal Hemoglobin