[Changes in aldolase and creatine kinase activity during thermal muscle damage].
A study was made of changes in outflux, extractability and enzyme (aldolase and creatine kinase) activity of muscle proteins induced by thermal action in skeletal muscles of R. temporaria L. Under a 15-minutes action of temperatures under 36 degrees C, which do not produce any contracture or fall of excitability, changes in a fraction of protein outfluxed from muscles were observed. The thermal action accompanied by the fall and irreversible loss of excitability (above 36 degrees) resulted in the fall of extractability and enzyme activity of water-soluble proteins extracted from homogenized muscles in addition to prolonged changes in the fraction of outfluxed proteins. The increased binding of aldolase by actomyosin under thermal injury of muscle is established. Changes of outflux extractability and enzyme activity of proteins during the thermal alteration of muscle are considered with regard to data about the complexing of muscle proteins.
D000818:Animals; D001001:Anura; D003402:Creatine Kinase / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D006358:Hot Temperature / Q000009:adverse effects*; D009132:Muscles / Q000201:enzymology / Q000293:injuries*; D013696:Temperature
D003402:Creatine Kinase; D005634:Fructose-Bisphosphate Aldolase
Izmeneniia al'dolaznoĭ i kreatinkinaznoĭ aktivnosti v khode teplovogo povrezhdeniia myshts.