[Comparative study of different nutrient media used for the isolation of Salmonella in routine laboratories].
For a comparative study on the merits of Selenit broth and Enterobacteriaceae-enrichment broth (EE-broth) by MOSSEL, the media were inoculated with 629 faecal cultures obtained from Salmonella carriers and contacts of a Salmonellosis sufferer. The Selenit broth incubated at 37 degrees C showed better results than the EE-broth incubated at 30 degrees C. After 24 hrs incubation plating was done on XLD-, SS-, and MACCONKEY-agarplates with the result that the XLD plate gave the highest yield of 21% isolations, followed by the SS-plate showing 17%, whilst the MACCONKEY-plate produced only 7%. In our routine laboratory the XLD-plate has now superseded the LEIFSON-plate and is used in parallel to the SS-plate after enrichment in Selenit-broth.
D003470:Culture Media; D006801:Humans; D012475:Salmonella / Q000302:isolation & purification*
D003470:Culture Media
Vergleichende Untersuchungen mit verschiedenen Nährmedien zur Isolierung von Salmonellen in der Routine-Diagnostik