[Tryptic hydrolysis extended to the level of aspartyl bonds].
A quantitative modification of free carboxyl groups in peptides and proteins can be obtained, under mild conditions, by reacting them with ethylenediamine in the presence of N-ethyl-N'-(3-dimethylaminopropyl)-Carbodimide. Aminoethylasparagine and aminoethylglutamine side chains are thus generated in place of the corresponding carboxylic ones. The first kind of residue because of its structure closer to that of lysine, is a point of greater potential trypsic cleavage than the second one. The specificity and yields of this enzymatic cleavage reaction and its possible application in sequence studies are discussed.
D000595:Amino Acid Sequence; D001224:Aspartic Acid; D005029:Ethylenediamines; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification*; D014357:Trypsin
D005029:Ethylenediamines; D010446:Peptide Fragments; D001224:Aspartic Acid; D014357:Trypsin
Hydrolyse trysique étendue au niveau des liaisons aspartyl