title: Molecular and catalytic properties of ribulose 1,5-bisphosphate carboxylase from the photosynthetic extreme halophile Ectothiorhodospira halophila.
abstract: D-Ribulose 1,5-bisphosphate (RuBP) carboxylase has been purified from the photosynthetic extreme halophile Ectothiorhodospira halophila. Despite a growth requirement for almost saturating sodium chloride in the medium, both crude and homogeneous preparations of RuBP carboxylase obtained from this organism were inhibited by salts. Sedimentation equilibrium analyses showed the enzyme to be large (molecular weight: 601,000). The protein was composed of two types of polypeptide chains of 56,000 and of 18,000 daltons. The small subunit appeared to be considerably larger than the small subunit obtained from the RuBP carboxylase isolated from Chromatium, an organism related to E. halophila. Amino acid analyses of hydrolysates of both E. halophilia and Chromatium RuBP carboxylases were very similar. Initial velocity experiments showed that the E. halophila RuBP carboxylase had a Km for ribulose diphosphate of 0.07 mM and a Km for HCO3- of 10 mM. Moreover, 6-phospho-D-gluconate was found to markedly inhibit the E. halophila carboxylase; a Ki for phosphogluconate of 0.14 mM was determined.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002262:Carboxy-Lyases / Q000378:metabolism; D002474:Cell-Free System; D002841:Chromatiaceae / Q000201:enzymology* / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D010788:Photosynthesis; D011189:Potassium Chloride / Q000494:pharmacology; D012273:Ribulose-Bisphosphate Carboxylase / Q000032:analysis / Q000378:metabolism; D012274:Ribulosephosphates / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D005942:Gluconates; D010455:Peptides; D012274:Ribulosephosphates; D012965:Sodium Chloride; D011189:Potassium Chloride; D002262:Carboxy-Lyases; D012273:Ribulose-Bisphosphate Carboxylase