Fluorogenic labeling of organophosphate pesticides with dansyl chloride. Application to residue analysis by high-pressure liquid chromatography and thin-layer chromatography.
The analysis of some organophosphorus pesticides by fluorogenic labeling with dansyl chloride (5-dimethylaminonaphthalene--l-sulfonyl chloride) was investigated. The pesticides were hydrolysed in sodium hydroxide to the corresponding phenols. The reaction of dansyl chloride with the phenols was accomplished in a two-phase system. The resulting fluorescent derivatives were separated and analysed quantitatively by in situ thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC). As little as 10-25 ng/spot of pesticide was detected by both TLC and HPLC.
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds; D006868:Hydrolysis; D007306:Insecticides / Q000032:analysis*; D009943:Organophosphorus Compounds; D010573:Pesticide Residues / Q000032:analysis; D010636:Phenols; D013050:Spectrometry, Fluorescence
D003619:Dansyl Compounds; D007306:Insecticides; D009943:Organophosphorus Compounds; D010573:Pesticide Residues; D010636:Phenols