Determination of warfarin in animal relicta and feedingstuffs by  high-pressure liquid chromatography with confirmation of identity by mass spectrometry.
The determination of warfarin in liver, blood, urine and feedingstuffs by high-pressure liquid chromatography (HPLC) is described. Clean-up is simple and the lower limit of determination is about 0.025 ppm. Recoveries from liver are about 85% over the range 0.025-1.0 ppm; recoveries from blood range from about 67 to 90%. Chemical ionisation mass spectrometry can be used to confirm identity and, by specific ion monitoring, to detect warfarin in HPLC eluates at levels below the sensitivity of the HPLC detector.
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000032:analysis; D013058:Mass Spectrometry; D008722:Methods; D008832:Microchemistry; D014859:Warfarin / Q000032:analysis* / Q000097:blood / Q000652:urine
D014859:Warfarin