The attachment and penetration of T7 DNA/phage in Syrian hamster embryonic cells.
Bacteriophage T7 DNA can penetrate Syrian hamster embryonic cells after a mandatory initial pretreatment with DEAE-dextran. In 3 h an extracellular complex between T7DNA and the cell monolayer is formed which is equivalent to 105 T7 genomes per cell. During the ensuing 24-48 h of cell growth, an average of 102-103 T7 genomes are transported to the nucleus in 90% of the cells of the culture.
D001665:Binding Sites; D001692:Biological Transport; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D003090:Coliphages / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism*; D003851:Deoxyribonucleases; D005796:Genes; D007700:Kinetics; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D014779:Virus Replication
D004279:DNA, Viral; D003851:Deoxyribonucleases