Inhibition of ribulose-1,5-bisphosphate carboxylase/oxygenase by xylulose 1,5-bisphosphate.
Xylulose 1,5-bisphosphate is a potent inhibitor of ribulose-1,5-bisphosphate carboxylase/oxygenase. The enzyme was inhibited 50% at a xylulose 1,5-bisphosphate concentration of 0.56 micrometer and an enzyme concentration of 0.14 micrometer. When both ribulose 1,5-bisphosphate and xylulose 1,5-bisphosphate were added simultaneously to the enzyme, this inhibition appeared to be competitive. A preincubation of 20 to 30 min was needed for maximum inhibition. The inhibitory effect of this compound is probably exerted through its binding at the ribulose 1,5-bisphosphate active site, since both the carboxylase and oxygenase activities were inhibited similarly.
D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D010105:Oxygenases / Q000037:antagonists & inhibitors*; D010428:Pentosephosphates / Q000494:pharmacology*; D010944:Plants / Q000201:enzymology; D012273:Ribulose-Bisphosphate Carboxylase / Q000037:antagonists & inhibitors*; D014996:Xylulose / Q000494:pharmacology
D010428:Pentosephosphates; D014996:Xylulose; D010105:Oxygenases; D002262:Carboxy-Lyases; D012273:Ribulose-Bisphosphate Carboxylase