Chemical characterization and distribution of ABO blood group active glycoprotein in human erythrocyte membrane.
Occurrence and distribution of glycoprotein H substances in blood type O human erythrocyte membrance were studied using an alpha N-acetylgalactosaminyltransferase (A-enzyme) purified from milk of blood type A women. Erythrocytes of blood group O, nonsecretors and secretors alike, contain H substances that serve as a substrate for this transferase, and the H determinants seem to occur in all three erythrocyte membrane glycoproteins (periodic acid-Schiff (PAS)-1, PAS-2, and PAS-3). Structural studies of the A glycoproteins obtained by the transferase action, suggested that all erythrocyte H glycoproteins contain only type 2 antigenic determinant.
D000017:ABO Blood-Group System; D001789:Blood Group Antigens; D002462:Cell Membrane / Q000032:analysis; D004912:Erythrocytes / Q000032:analysis*; D005260:Female; D005643:Fucose / Q000032:analysis; D006023:Glycoproteins / Q000097:blood; D006602:Hexosyltransferases / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007785:Lactose / Q000032:analysis; D008895:Milk, Human / Q000201:enzymology; D009844:Oligosaccharides / Q000032:analysis; D011247:Pregnancy
D000017:ABO Blood-Group System; D001789:Blood Group Antigens; D006023:Glycoproteins; D009844:Oligosaccharides; D005643:Fucose; D006602:Hexosyltransferases; D007785:Lactose