title: Inhibition of transfer ribonucleic acid nucleotidyl transferase (EC 2.7.7.25) from Ehrlich tumor cells by proflavine sulfate and ethidium bromide.
abstract: Transfer RNA nucleotidyl transferase from Ehrlich tumor cells was inhibited by proflavine sulfate and ethidium bromide but not by actinomycin D, chromomycin, rifamycin SV, rifampin, daunomycin, or alpha-amanitin. Complete inhibition of nucleotidyl transferase activity was attained at a final concentration of 1 mmol/1 proflavine sulfate, while 2 mmol/1 ethidium bromide was required to completely inhibit the enzyme. CMP incorporation into tRNA was more sensitive to both proflavine sulfate and ethidium bromide than was AMP incorporation.
mesh_terms: D000166:Acridines / Q000494:pharmacology*; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D003597:Cytosine Nucleotides / Q000378:metabolism; D004996:Ethidium / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D011370:Proflavine / Q000494:pharmacology*; D012316:RNA Nucleotidyltransferases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification
chemical_list: D000166:Acridines; D000900:Anti-Bacterial Agents; D003597:Cytosine Nucleotides; D000249:Adenosine Monophosphate; D011370:Proflavine; D012316:RNA Nucleotidyltransferases; D004996:Ethidium