Reactivity of snail agglutinin from Helix pomatia (anti-AHP) to non-A meconium glycoproteins.
Investigations have been made on the reactivity of snail agglutinin from Helix pomatia (anti-AHP) with blood group active neutral and acid glycoprotein (NGP and AGP) prepared from non-A meconium. It is clearly demonstrated that anti-AHP reacts only with NGP, but not with AGP, in anti-AHP-non-A meconium system. After desialization, however, the AGP was reactive to anti-AHP. The effects of alpha-N-acetyl-D-galactosaminase, blood group decomposing enzymes and mild alkaline treatment on the reactivity of NGP and desialized AGP (DAGP) were studied by the immunochemical techniques and the structure responsible for the reaction is briefly discussed: the occurrence of terminal or subterminal alpha-D-GalNAc residues as reactant to anti-AHP may be deduced.
D000373:Agglutinins; D000818:Animals; D000906:Antibodies; D001789:Blood Group Antigens; D006023:Glycoproteins / Q000276:immunology*; D006372:Helix, Snails / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D008470:Meconium / Q000276:immunology*; D011233:Precipitin Tests
D000373:Agglutinins; D000906:Antibodies; D001789:Blood Group Antigens; D006023:Glycoproteins