Removal of adsorbed elastase from partially digested elastin.
Bovine ligamentum nuchae elastin that had been partially digested by porcine pancreatic elastase and then thoroughly washed with 0.2 M-NaC1/0.05 M-sodium borate solution continued to release peptide fragments in solution. Complete removal of elastase from the elastin surface required extraction with dilute alkali, a procedure that does not irreversibly inactivate the enzyme or hydrolyse elastin.
D000818:Animals; D001881:Borates; D002417:Cattle; D004549:Elastin / Q000032:analysis*; D007700:Kinetics; D010196:Pancreatic Elastase / Q000302:isolation & purification*; D012995:Solubility
D001881:Borates; D004549:Elastin; D010196:Pancreatic Elastase