Adsorption of free steroids with charcoal-gelatin disks in radioimmunoassays.
The preparation of charcoal-gelatin disks and their use for removing free steroid in radioimmunoassays are described. Plasma concentrations of several steroids were determined using charcoal-gelatin disks or charcoal suspension and the results are compared. The advantages of using charcoal-gelatin disks are discussed.
D000327:Adsorption; D000735:Androstenedione / Q000097:blood; D000818:Animals; D002606:Charcoal; D005780:Gelatin; D006854:Hydrocortisone / Q000097:blood; D011374:Progesterone / Q000097:blood; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods*; D012756:Sheep; D013256:Steroids / Q000032:analysis*; D013739:Testosterone / Q000097:blood
D013256:Steroids; D002606:Charcoal; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D005780:Gelatin; D006854:Hydrocortisone