Ion-pair extraction method for quantitation of a bisquaternary ammonium compound, pancuronium bromide.
Pancuronium bromide (PCBr), a neuro-muscular blocking agent, was determined quantitatively in aqueous solution by ion-pair extraction. Separate determination of PCBr and degradation products was possible after thin layer chromatography. The procedure was developed by using a theoretical approach. Favorable conditions were calculated from the ion-pair extraction constant. The drug was determined with bromothymol blue at pH 9.0, using one extraction in the direct procedure and 2 successive extractions in the combined elution-extraction process after thin layer chromatography. In the direct method, 0.100 mg PCBr was determined with a reproducibility of +/- 1.0%.
D002855:Chromatography, Thin Layer; D008722:Methods; D010197:Pancuronium / Q000032:analysis*; D012996:Solutions / Q000032:analysis; D013053:Spectrophotometry
D012996:Solutions; D010197:Pancuronium