A simple radioassay for 25-hydroxycholecalciferol without chromatography.
A simple method to assay 25-hydroxycholecalciferol in human plasma without chromatography is described. The method includes ammonium sulphate precipitation of 25-hydroxycholecalciferol bound to plasma-transport globulins as a purification step, extraction with toluene and saturation analysis utilizing human osteomalacic plasma for competitive protein-binding assay. The criteria of reliability of the method are reported.
D000293:Adolescent; D000328:Adult; D004827:Epilepsy / Q000097:blood; D005069:Evaluation Studies as Topic; D006801:Humans; D006887:Hydroxycholecalciferols / Q000097:blood*; D008875:Middle Aged; D010018:Osteomalacia / Q000097:blood; D011869:Radioligand Assay / Q000379:methods
D006887:Hydroxycholecalciferols