title: RNA metabolism in chick oviduct during oestrogen stimulation.
abstract: The amount and the labelling of RNA were studied in chick oviduct after secondary stimulation of pre-treated chicks with oestrogen. The weight of the oviduct increases over 2 fold in 20 h of oestrogen action using a dose of 1 mg. During the hormonal treatment the amount of cytoplasmic and nuclear RNA increases about 3 and 1.5 fold, respectively. The amount of poly(A) containing RNA increases somewhat less than that of RNA lacking poly(A). The proportion of poly(A) containing species of the total cytoplasmic and nuclear RNA decreases from 5.8% to 4.4% and from 7.8% to 6.3%, respectively. Hydroxyurea largely prevents the effect of oestrogen in increasing the amount of RNA. Incorporation of [3H]uridine in RNA in vitro is stimulated about 2.5 fold in cytoplasmic RNA lacking poly(A) and only 1.2 fold in RNA containing poly(A) in 20 h of oestrogen action. In nuclear RNA the labelling of the two species is stimulated about 1.7 fold. Hydroxyurea fails to interfere with the oestrogen-induced stimulation in the labelling of RNA. Cytoplasmic and nuclear RNA containing poly(A) is distributed in a peak of about 20 S in agarose-acrylamide gels.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D003593:Cytoplasm / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006918:Hydroxyurea / Q000494:pharmacology; D009963:Orotic Acid; D010057:Oviducts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D014529:Uridine
chemical_list: D004958:Estradiol; D009963:Orotic Acid; D012313:RNA; D014529:Uridine; D006918:Hydroxyurea