Concentration-dependent dissociation of factor VIII in 1 M NaCl.
Plasma, cryoprecipitate, Hemofil, and human factor VIII concentrate were dissolved in 1.0 M NaCl and chromatographed on Bio-Gel A-5m. With high concentrations of factor VIII the activity eluted as a symmetrical peak in the void volume; with a low factor VIII concentration the procoagulant activity was retarded. Dilution curves were performed for several human factor VIII concentrates. When the concentration of factor VIII was decreased, elution patterns showed a gradual transition from a peak in the void volume to a peak with a Ve/Vo of 1.7. Cryoprecipitate exhibited a similar behavior in 1.0 M NaCl, but the percent dissociation was greater than expected at high concentrations of factor VIII. When gel filtration was performed with 0.25 M CaCl2, significant dissociation occurred at all concentrations of factor VIII tested. The behavior of factor VIII in 1.0 M NaCl closely fit a theoretically derived curve for the dissociation of a protein from its binder. We conclude that the dissociation of factor VIII in 1 M NaCl is dependent on the concentration and purification of the procoagulant protein.
D000818:Animals; D001779:Blood Coagulation Factors / Q000032:analysis; D002122:Calcium Chloride; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D005169:Factor VIII / Q000032:analysis / Q000378:metabolism; D006801:Humans; D012965:Sodium Chloride
D001779:Blood Coagulation Factors; D002352:Carrier Proteins; D012965:Sodium Chloride; D005169:Factor VIII; D002122:Calcium Chloride