The biochemical basis of nitroblue tetrazolium reduction in normal human and chronic granulomatous disease polymorphonuclear leukocytes.
Normal human polymorphonuclear leukocytes (PMN) placed in anaerobic chambers reaching pO2's of less than 5 mm Hg fail to generate O2-, iodinate ingested particles, and stimulate glucose-1-14C oxidation through the hexose monophosphate shunt. The observation that anaerobic cells are incapable of generating O2- or reducing nitroblue tetrazolium (NBT) to formazan supports the idea that NBT reduction in phagocytizing PMN is due exclusively to oxygen-dependent O2- generating oxidase which is deficient in chronic granulomatous disease leukocytes, despite their hyperphagocytic capacity.
D006105:Granulomatous Disease, Chronic / Q000097:blood*; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010585:Phagocyte Bactericidal Dysfunction / Q000097:blood*; D010587:Phagocytosis; D013778:Tetrazolium Salts / Q000378:metabolism*
D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium