Preparation of porcine pancreatic lipase free of co-lipase activity.
Porcine pancreatic lipase LB was purified and released from co-lipase activity by reduction with beta-mercaptoethanol in the presence of guanidine chloride. The amino acid and carbohydrate composition of the enzyme is presented. The following physical constants were measured or calculated: Molecular weight 52 000, sedimentation coefficient (s degrees 20, w) 4.0 S, diffusion coefficient (D degrees 20, w) 6.7 X 10(-7) cm2 s-1. Stokes' radius (r) 30.3 A, partial specific volume (v) 0.72 cm3 g-1, frictional ratio (f/f0) 1.23 and isoelectric point (pI) 5.18.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D003089:Colipases / Q000032:analysis; D008049:Lipase / Q000302:isolation & purification*; D008970:Molecular Weight; D010189:Pancreatic Juice / Q000201:enzymology*; D013552:Swine
D000596:Amino Acids; D002241:Carbohydrates; D003089:Colipases; D008049:Lipase