[Disturbances in the regulation of phospholipid metabolism of the whole liver, mitochondria and microsomes in acute thioacetamide poisoning and the influcence of silymarin].
In the whole liver of rats, pretreated with 4% polyvinylpyrrolidone solution, 24 h after i.p. application of 300 mg thioacetamide/kg body weight, an increase of total lipids, triglycerides and phospholipids is observed, in the mitochondrial fraction phospholipid concentrations are decreased. At the same time, phosphatidylcholine/phosphatidyl-ethanolamine ratio is diminished in mitochondrial and microsomal fraction. After administration of [l-14C]-palmitate, in comparison with non-intoxicated animals, a statistically significant reduction of specific radioactivity of phosphatidylethanolamine could be observed in microsomal and mitochondrial fraction; specific radioactivities of total lipids, total phospholipids and phosphatidyl choline trend to diminution in the whole liver and in subcellular fractions. By prophylactic i.p. application of silymarin-N-methylglucamine salt (400 mg/kg body weight), dissolved in 4% polyvinylpyrrolidone solution, there is a normalization of altered microsomal phosphatidylcholine/phosphatidylethanolamine ratio as well as an enhancement of diminished mitochondrial phosphatidylcholine concentration, while the reduction of specific radioactivities of phosphatidylethanolamine is partially abolished in the mitochondrial fraction.
D000081:Acetamides / Q000506:poisoning*; D000818:Animals; D005419:Flavonoids / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D010168:Palmitates / Q000008:administration & dosage; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D012838:Silymarin / Q000494:pharmacology*; D013853:Thioacetamide / Q000506:poisoning*; D014280:Triglycerides / Q000378:metabolism
D000081:Acetamides; D005419:Flavonoids; D010168:Palmitates; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012838:Silymarin; D014280:Triglycerides; D013853:Thioacetamide
Regulationsstörungen des Phospholipidstoffwechsels der Gesamtleber, Mitochondrien und Mikrosomen bei der akuten Intoxikation durch Thioacetamid und deren Beeinflussung durch Silymarin