Axenic cultivation of Phytomonas davidi Lafont (Trypanosomatidae), a symbiote of laticiferous plants (Euphorbiaceae).
Phytomonas davidi (Trypanosomatidae) originally discovered by Lafont in 1909 on the island of Mauritius was rediscovered in Euphorbia cyathophora in Florida. Successful cultures were established in diphasic medium consisting of duck blood agar and modified Phillips' medium as overlay. Optimal growth was obtained when Mansour's medium was used as overlay and poorest growth when Cowperthwaite's medium buffered at pH 5.0 was utilized for this purpose. Marked changes tending toward choanomastigotes rather than the elongate twisted promastigotes were observed in cultures.
D003470:Culture Media; D056890:Eukaryota / Q000166:cytology / Q000254:growth & development*; D010944:Plants / Q000469:parasitology*; D013559:Symbiosis
D003470:Culture Media