Surface charge of erythrocytes in paroxysmal nocturnal haemogloninuria.
The electrophoretic mobility of erythrocytes from 3 patients with PNH was measured by standard techniques. Erythrocyte mobility is a measure of the net negative surface charge of these cells. In each of the 3 patients studied in mobility was significantly increased over normal cells indicating that there is an increase in negative surface charge of PNH erythrocytes. Following the Ham's and sucrose lysis tests; surviving cells showed mean mobilities that were not statistically different from normal cells. This suggests that these cells with higher surface charge are vulnerable to complement lysis.
D002462:Cell Membrane / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D004586:Electrophoresis; D004905:Erythrocyte Aging / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D005978:Glutathione / Q000494:pharmacology; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans
D005978:Glutathione