Electrophoretic modifications of three enzymes in extracts of human and bovine lens. Posttranslational "aging" of lens enzymes.
Electrophoretic modifications have been found in extracts from human and bovine lenses for three enzymes: glucose-6-phosphate dehydrogenase, triosephosphate isomerase and nucleoside phosphorylase. Increased anodic mobility is observed in all cases. It is more pronounced than in red cell lysates, also more evident in lenses from adult than from young animals. These results give evidence of posttranslational "aging" of enzyme molecules in lenses.
D000375:Aging; D000818:Animals; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D002417:Cattle; D004912:Erythrocytes / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D007908:Lens, Crystalline / Q000201:enzymology* / Q000254:growth & development; D010430:Pentosyltransferases / Q000378:metabolism*; D013997:Time Factors; D014305:Triose-Phosphate Isomerase / Q000378:metabolism*
D005954:Glucosephosphate Dehydrogenase; D010430:Pentosyltransferases; D002238:Carbohydrate Epimerases; D014305:Triose-Phosphate Isomerase