title: Studies on sperm antigenicity, 5. In vivo and in vitro cellular reactivity in guinea pigs sensitized with fractions of human spermatozoa.
abstract: In vitro delayed type hypersensitivity was demonstrated with peritoneal exudate cells from guinea pigs immunized with different preparations of human and guinea pig seminal components emulsified with complete Freund's adjuvant-H37Ra. The seminal components were intact human spermatozoa (HuSp); intact guinea pig spermatozoa (GPSp); human seminal plasma (HuSePlFr); and fractions of human spermatozoa obtained by centrifugation of the homogenate at 5,000 X g (5S30 and 5p30), at 20,000 X g (20S30 and 20p30), and at 144,000 X g (144p120). Cellular sensitivity was demonstrated in vivo by skin testing and in vitro by the macrophage inhibition technique. Peritoneal exudate cells from guinea pigs sensitized with fractions 5p30 and 20p30 elicited a delayed hypersensitivity reaction which could be detected only with intact human spermatozoa. Other human spermatozoal fractions (5S30, 20S30, and 144p120) were weak immunogens. Sensitization of guinea pigs with fractions of human spermatozoa, in addition to causing delayed hypersensitivity reactions, elicited low titers of spermatoxic antibodies. Antibodies to human spermatozoal fractions 5S30, 5p30, 20S30, and 20p30 cross-reacted with intact human spermatozoa and intact guinea pig spermatozoa. It is postulated that the existence of "spermatozoa-specific" coating antigen(s) derived from other components of the reproductive tract might be responsible for human spermatozoal antigenicity.
mesh_terms: D000818:Animals; D000906:Antibodies; D000941:Antigens; D002458:Cell Fractionation; D002464:Cell Migration Inhibition; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D006168:Guinea Pigs; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000473:pathology; D008297:Male; D012661:Semen / Q000276:immunology; D013045:Species Specificity; D013094:Spermatozoa / Q000276:immunology*; D013347:Subcellular Fractions / Q000276:immunology; D013737:Testis / Q000473:pathology
chemical_list: D000906:Antibodies; D000941:Antigens