An adjuvant effect of poly A - poly U on production of antibodies to DNA in rabbits.
The effect of polyadenylate-polyuridylate copolymer (poly A-poly U) on the formation of anti-DNA antibodies in rabbits was investigated. 1) Poly A-poly U proved to be an effective adjuvant for production of antibodies to DNA. These were elicited in rabbits at high titer and were formed at an early stage of immunizations. 2) The antiserum obtained reached potently with denatured DNA, but with neither methylated bovine serum albumin (MBSA) used as carrier protein nor poly A-poly U used as adjuvant. This was demonstrable both by C-fixation and double diffusion tests. 3) The antibodies occurred exclusively in the IgG fraction separated by Sephadex G-200 column chromatography.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D004247:DNA / Q000008:administration & dosage / Q000276:immunology*; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007074:Immunoglobulin G / Q000032:analysis; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D011063:Poly A-U / Q000494:pharmacology*; D011817:Rabbits; D012709:Serum Albumin / Q000008:administration & dosage
D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D012709:Serum Albumin; D011063:Poly A-U; D004247:DNA; D005620:Freund's Adjuvant