Partial purification of human renin substrate.
A two-step purification method is described for the preparation of renin substrate from human plasma. Pooled plasma from women on oral contraceptives is used for the purification. The overall yield of renin substrate is 57%, with a twenty-fold purification. The specific renin substrate content of the preparation, as determined by enzymatic degradation with an excess of human renin, is 2 mug angiotensin I per mg protein. The product has a very low endogenous renin activity and is free from angiotensinase activity. An additional purification step involving affinity chromatography is described. In pilot studies a renin substrate yield of 37% has been achieved, with a hundred-fold purification. The final product has a specific renin substrate content of 10 mug angiotensin I per mg protein. The preparation contains up to 12 different plasma proteins, nine of which have been identified and quantitated.
D000804:Angiotensin II / Q000031:analogs & derivatives*; D000808:Angiotensinogen / Q000032:analysis / Q000302:isolation & purification*; D001798:Blood Proteins / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods; D004355:Drug Stability; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007700:Kinetics; D008722:Methods
D001798:Blood Proteins; D000808:Angiotensinogen; D000804:Angiotensin II