A radioimmunoassay for antibodies against surface membrane antigens using adhering cells.
A radioimmunoassay using cells adhering to plastic is described. In this assay, A-10 mammary carcinoma attached to the surface of plastic in microtiter plates were permitted to bind antibody and the bound antibody was detected with purified rabbit 125I-anti-mouse-Fab. The bound radioactive material was eluted with glycine-HCI buffer (pH 2.5), and the acid eluates were counted in a gamma counter. This assay can be used to detect cytolytic or noncyctolytic antibody to cell surface antigens in studies with any tumor or normal cell that will adhere to a solid surface.
D000230:Adenocarcinoma / Q000276:immunology; D000818:Animals; D001666:Binding Sites, Antibody; D002448:Cell Adhesion; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D011863:Radioimmunoassay