[Restoration of ability to proliferate and produce mitogenic factor by human lymphocytes in a serum-free medium following their purification].
The response of purified and "non-purified" human blood lymphocytes to phytoheamagglutinin (PHA) and their capacity to produce mitogenic factor upon PHA-stimulation in protein-free and serum-containing culture medium were investigated. Non-purified lymphocytes lost the capacity to react to the stimulation with PHA and to produce the mitogenic factor in protein-free culture medium. Purification of lymphocytes on the density gradient or passing them through a column made them resume their response to PHA and their capacity to produce the mitogenic factor in protein-free medium. It is assumed that the inhibition of the response to PHA in protein-free culture medium may be caused by polymorphonuclear leucocytes.
D001798:Blood Proteins; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis*; D008934:Mitogens
D001798:Blood Proteins; D003470:Culture Media; D008222:Lymphokines; D008934:Mitogens
Vosstanovlenie sposobnosti k proliferatsii i produktsii mitogennogo faktora limfotsitami cheloveka v bessyvorotochnoĭ srede posle ikh ochistki