New rapid determination of pyridoxal phosphate using tyrosine phenol-lyase.
A rapid, specific, and precise spectrophotometric assay for the determination of pyridoxal phosphate is described. The assay allows for the determination of the cofactor between 0.1 and 1.0 microgram/ml. Its applicability to pyridoxal phosphate in biological fluids was demonstrated by a determination of the plasma half-life in BDF1 mice. Pyridoxal phosphate is absorbed rapidly from the peritoneal cavity and cleared from the plasma with a half-life of about 15 min.
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D005260:Female; D006207:Half-Life; D008190:Lyases; D008722:Methods; D051379:Mice; D008815:Mice, Inbred Strains; D011732:Pyridoxal Phosphate / Q000032:analysis* / Q000097:blood; D013997:Time Factors; D014447:Tyrosine Phenol-Lyase
D001798:Blood Proteins; D011732:Pyridoxal Phosphate; D008190:Lyases; D014447:Tyrosine Phenol-Lyase