Gas-liquid chromatographic determination of cholesterol and other sterols in foods.
A gas-liquid chromatographic (GLC) method, using the butyryl esters of sterols, has been developed for the measurement of cholesterol, stigmasterol, sitosterol, and campesterol in foods. An immobile phase of 1% SE-30 coated on 100-120 mesh Gas-Chrom Q packed in a 6 inch X 4 mm id glass column operated at 255 degrees C was the most satisfactory of 7 column packings evaluated. Extraction with chloroform-methanol gave 98.7% recovery with a coefficient of variation of 1.8% for cholesterol added to a variety of foods. When cholesteryl palmitate was added to vegetable oil and the butyryl derivative was prepared, followed by GLC analysis, the recovery was 99.3% with a coefficient of variation of 0.9%. Amounts as low as 1 mg/100 g food can be detected with a precision of 2.5%. The results of the analysis of a variety of foods for cholesterol, campesterol, sitosterol, and stigmasterol are given.
D002784:Cholesterol / Q000031:analogs & derivatives / Q000032:analysis*; D002788:Cholesterol Esters / Q000032:analysis; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D005504:Food Analysis; D008722:Methods; D010840:Phytosterols / Q000032:analysis*; D012855:Sitosterols / Q000032:analysis; D013265:Stigmasterol / Q000032:analysis
D002788:Cholesterol Esters; D010840:Phytosterols; D012855:Sitosterols; C021273:campesterol; D002784:Cholesterol; D013265:Stigmasterol