The ribosomes of Drosophila. Normal and defective ribosome biosynthesis in Drosophila cell cultures.
The assembly of proteins and RNA into mature ribosomal subunits has been studied in Drosophila cell cultures by pulse-chase experiments. Pulse labeled rRNA has a transit time of 3 h, while the transfer of ribosomal protein occurs completely within 30 min. Inhibition of protein synthesis by cycloheximide results in an almost immediate cessation of ribosome assembly, a result which indicates that no large pool of free ribosomal proteins exists in the cell. Substituting pre-ribosomal RNA with the analogue 5-fluorouridine (5-FU) results in a cessation of ribosome muturation. Under these conditions at least three large subunit proteins continue to accumulate on pre-existing cytoplasmic subunits, indicating an exchange. A portion of ribosomal subunit proteins synthesized in the presence of 5-FU can be recovered in cytoplasmic subunits once the effect of 5-FU has been reversed. This is most easily interpreted in terms of their stabilization on substituted pre-rRNA within the nucleolus, and subsequent utilization on unsubstituted RNA.
D000241:Adenosine / Q000378:metabolism; D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004330:Drosophila; D005472:Fluorouracil / Q000494:pharmacology*; D066298:In Vitro Techniques; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012269:Ribosomal Proteins / Q000096:biosynthesis*; D012270:Ribosomes / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D003513:Cycloheximide; D000241:Adenosine; D005472:Fluorouracil; D014529:Uridine