A stopped-flow dual-wavelength spectrophotometer suitable for the study of respiratory chains.
The requirements for a dual-wavelength stopped-flow spectrophotometer to be suitable for studying limited quantities of respiratory-chain preparations are described. They can be met by a design using mainly commercially available components. The constructed apparatus has a dead-time of approx. 2.6 ms, a mixing ratio of 17:1, and a minimal requirement for 0.5 ml of mixed reactants per flow.
D003580:Cytochromes / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D013053:Spectrophotometry / Q000295:instrumentation*; D013997:Time Factors
D003580:Cytochromes