Regulation and metabolic background of polyketide formation. I. Effects of (-)-hydroxycitrate and metabolic roles of citrate and malate in fatty acid and polyketide formations.
The effects of (-)-hydroxycitrate on fatty acid and alternariol syntheses from glucose and acetate in Alternaria alternata were investigated. Fatty acid synthesis from glucose and acetate was inhibited by (-)-hydroxycitrate. The inhibition could partly be removed by the addition of malate or isocitrate. Alternariol synthesis from glucose was also inhibited by (-)-hydroxycitrate. This inhibition was not influenced to the same extent by malate addition as was that of fatty acid synthesis. It is shown that ATP -citrate lyase is essential not only for the production of cytoplasmic acetyl-CoA but also for the production of NADPH by supplying malate via oxaloacetate.
D000085:Acetates / Q000378:metabolism; D000528:Alternaria / Q000378:metabolism*; D002951:Citrates / Q000494:pharmacology*; D004347:Drug Interactions; D005227:Fatty Acids / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D007523:Isocitrates / Q000494:pharmacology; D007783:Lactones / Q000096:biosynthesis*; D008293:Malates / Q000494:pharmacology; D003904:Mitosporic Fungi / Q000378:metabolism*
D000085:Acetates; D002951:Citrates; D005227:Fatty Acids; D007523:Isocitrates; D007783:Lactones; D008293:Malates; D005947:Glucose; C005197:alternariol