The effect of respiratory immunization on cell-mediated immune effector cells of the lung.
In order to understand the mechanisms of cellular immune injury in hypersensitivity pneumonitis, the effect of type of antigen on cell-mediated immunity in guinea-pigs receiving respiratory immunization was studied. Lymphocytes obtained by pulmonary lavage were compared with those from peritoneal exudate following immunization with either a soluble protein, human serum albumin, or a particulate suspension of Thermoactinomyces vulgaris. Assays were obtained without mixing cells from these two sources. Statistically significant increases (13-22%) in the number of alveolar rosette-forming cells (RFC) were found in the animals immunized with either antigen, but only the particulate T. vulgaris was also capable of inducing a systemic increase of such cells. That this increase in RFC could be due to specifically reactive lymphocytes was demonstrated by the production of antigen-stimulated macrophage migration inhibition. Some evidence was obtained that indicated that T. vulgaris could act both as a non-specific B-cell stimulant and a specific T-cell activator. The concept of a hypothetical pulmonary 'barrier' is discussed which must be overcome to induce systemic immune responses following respiratory immunization. T. vulgaris must be added to the list of known agents or means for overcoming this 'barrier'.
D000336:Aerosols; D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D002464:Cell Migration Inhibition; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D007104:Immune Adherence Reaction; D007111:Immunity, Cellular; D007114:Immunization; D008168:Lung / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008848:Micromonosporaceae / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology
D000336:Aerosols; D012709:Serum Albumin