A method for the measurement of ultra-low amounts of prostaglandins in inflammatory exudates using gas chromatography and mass spectrometry.
A GC-MS assay for PGE2 and PGF2alpha has been described. The method is highly specific and sensitive down to the sub-picogram level. The assay has been used to measure the time dependence of PG concentration in carrageenan-induced inflammatory exudates and the results compared with a bioassay. Estimates of the extraction and purification yield of PGs from exudates have also been obtained.
D000818:Animals; D001681:Biological Assay; D002351:Carrageenan; D002849:Chromatography, Gas; D005122:Exudates and Transudates / Q000032:analysis*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D013058:Mass Spectrometry; D008722:Methods; D011453:Prostaglandins / Q000032:analysis*; D011458:Prostaglandins E / Q000302:isolation & purification; D011460:Prostaglandins F / Q000302:isolation & purification; D051381:Rats; D013997:Time Factors
D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D002351:Carrageenan