title: Specificity of ethacrynic acid as a sulfhydryl reagent.
abstract: Ethacrynic acid is not an absolutely specific SH-reagent. It reacts rapidly and reversibly with cysteine, imidazole, histidine, and lysine. The reaction products increase with increasing pH. LDH is rapidly and reversibly inhibited by ethacrynic acid.
mesh_terms: D003545:Cysteine; D004976:Ethacrynic Acid; D006639:Histidine; D007093:Imidazoles; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors; D008239:Lysine; D012694:Serine; D013439:Sulfhydryl Reagents
chemical_list: D007093:Imidazoles; D013439:Sulfhydryl Reagents; D012694:Serine; D006639:Histidine; D007770:L-Lactate Dehydrogenase; D008239:Lysine; D003545:Cysteine; D004976:Ethacrynic Acid