[Isolation, purification and several properties of the extracellular RNA-ase of Bacillus amylozyma].
RNase has been isolated from the cultural broth of Bacillus amylozyma 9a. The dynamics of accumulation of this exocellular enzyme was studied during the growth of the bacterial population, and the optimal conditions for production of the RNase were determined. The enzyme was isolated and purified as follows: precipitation with ammonium sulphate, dialysis, thermal treatment of the enzyme solution, chromatography on DEAE- and CM-celluloses. The enzyme has been purified by 274 times; its specific activity is 3300 units. The preparation contains no DNase, PDEase, and PMEase. The following physical and chemical properties of the enzyme has been studied: molecular weight, thermostability, pH otimum, activation with metal ions.
D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D003013:Clostridium / Q000201:enzymology*; D004789:Enzyme Activation; D008274:Magnesium; D008722:Methods; D011188:Potassium; D012260:Ribonucleases / Q000096:biosynthesis* / Q000302:isolation & purification; D012964:Sodium
D012964:Sodium; D012260:Ribonucleases; D008274:Magnesium; D011188:Potassium
Vydelenie, ochistka i nekotorye svoĭstva vnekletochnoĭ RNKazy Bacillus amylozyma