Influence of pyridoxine supplementation on vitamin B-6 levels in milk of rats deficient in the vitamin.
Levels of vitamin B-6 in milk from pyridoxine deficient dams were used as an indicator of the ability of pyridoxine to protect offspring against the effects of the deficiency. Sprague Dawley rats were fed a basal diet containing 30.0 (control) or 1.2 (deficient) mg pyridoxine-HC1/kg diet from weaning throughout growth, gestation and until 5 days postpartum. At this time, deficient dams were supplemented by a single intraperitoneal injection of 600 mug pyridoxine-HC1, or by adding 30 or 60 mg pyridoxine-HC1/kg to the diet. The vitamin B-6 content in milk form the group supplemented by injection exceeded the control level of 38.8 mug/100 ml milk 30 minutes after the injection, and reached a peak level of 110.7 mug/100 ml at 4 hours with a subsequent decline to 27mug/100 ml at 20 hours. In rats supplemented orally with 30 or 60 mg pyridoxine-HC1/kg diet, the vitamin B-6 level in the milk reached the control value in 24 and 6 hours, respectively. At 120 hours, orally supplemented dams had significantly higher levels of vitamin B-6 in the milk than control animals. Vitamin supplementation of dams by a single injection of pyridoxine-HC1 was sufficient to overcome the pyridoxine deficiency syndrome in the pups, but was not adequate for optimum growth.
D000818:Animals; D001835:Body Weight; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005260:Female; D007774:Lactation; D008297:Male; D008892:Milk / Q000378:metabolism*; D011247:Pregnancy; D011736:Pyridoxine / Q000378:metabolism / Q000627:therapeutic use; D051381:Rats; D026681:Vitamin B 6 Deficiency / Q000188:drug therapy / Q000378:metabolism* / Q000517:prevention & control
D011736:Pyridoxine