title: Immunoglobulins of the chicken antibody to Newcastle disease virus (Mukteswar and F strain).
abstract: The qualitative and quantitative development of chicken immunoglobulins in response to R2B (Mukteswar) and F strain of Newcastle disease virus was studied. One primary (R2B) and two secondary (R2B - R2B, F - R2B) vaccination trials were conducted. Sera was collected at weekly intervals and analyzed. There was an increase in total serum protein content in parallel to an increase in serum neutralizing (SN), hemagglutination inhibition (HI), and precipitating antibodies. The SN, HI and precipitating activities were detected both in IgM and IgG immunoglobulins when sera were treated with mercapto-ethanol. However, Sephadex G-200 fractionated sera showed only SN activity in the IgM fraction, whereas the IgG fraction showed both HI and SN activity. Serum IgM antibodies appeared during the first week following vaccination, then diminished, then rose again following secondary vaccination. Apparently, immunoglobulin induction and serological activities were not significantly influenced by the age of the chickens.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000917:Antibody Formation; D001798:Blood Proteins / Q000032:analysis; D002645:Chickens / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D008297:Male; D009522:Newcastle disease virus / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral; D001798:Blood Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins