Salivary epithelial cells in primary culture: characterization of their growth and functional properties.
Mouse submandibular salivary gland cells were grown in primary explant culture. After an initial period of degeneration within the explant, surviving epithelial cells proliferated rapidly and duct-like structures recolonized the explant. Autoradiographic studies showed that a peak of DNA synthesis occurred after 4 days in vitro and that proliferation was enhanced by insulin and hydrocortisone. These cells retained specialized secretory function (protease activity) for at least 2 weeks in vitro. This enzyme is a differentiated product of granular tubule cells in vivo. Between 6 and 10 days, explants attached to the substrate. An outgrowth developed, consisting largely of ultrastructurally identifiable epithelial cells which formed pseudoglandular structures in the monolayer. Epithelium survived for over 6 months in primary culture but could not be serially transferred. Secondary cultures were rapidly overgrown by mesenchymal cells.
D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D002465:Cell Movement; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism; D003470:Culture Media; D003896:Desmosomes / Q000648:ultrastructure; D004847:Epithelial Cells; D006651:Histocytochemistry; D006854:Hydrocortisone; D006913:Hydroxysteroid Dehydrogenases / Q000032:analysis; D007328:Insulin; D007365:Intercellular Junctions / Q000648:ultrastructure; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000209:etiology; D010447:Peptide Hydrolases / Q000032:analysis; D013363:Submandibular Gland / Q000166:cytology*; D013739:Testosterone / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013974:Thyroxine / Q000494:pharmacology
D003470:Culture Media; D007328:Insulin; D013739:Testosterone; D006913:Hydroxysteroid Dehydrogenases; D010447:Peptide Hydrolases; D007545:Isoproterenol; D013974:Thyroxine; D013936:Thymidine; D006854:Hydrocortisone