title: Further evidence of X-linkage of hypoxanthine phosphoribosyl-transferase in the mouse.
abstract: A variant of the HPRT-A9 mouse cell line was fused with wild type diploid mouse bone marrow cells to obtain an HPRT+ line. The unique chromosomal features of the A9 parent, including the presence of a t(X;3) translocation and the absence of normal chromosomes 15,16, 17, 18, and the X, have permitted use of this intraspecific hybrid for chromosome mapping. Back-selection of hybrid cells in 8-azaguanine for loss of HPRT resulted in the loss of the Xchromosome derived from the diploid parent, providing evidence of the X-linkage of the HPRT locus.
mesh_terms: D000818:Animals; D001375:Azaguanine / Q000494:pharmacology; D001853:Bone Marrow / Q000648:ultrastructure; D001854:Bone Marrow Cells; D002460:Cell Line; D002478:Cells, Cultured; D002874:Chromosome Mapping; D003470:Culture Media; D004171:Diploidy; D005796:Genes; D008040:Genetic Linkage; D006822:Hybrid Cells / Q000187:drug effects; D007041:Hypoxanthine Phosphoribosyltransferase; D051379:Mice; D012730:Sex Chromosomes; D014178:Translocation, Genetic
chemical_list: D003470:Culture Media; D007041:Hypoxanthine Phosphoribosyltransferase; D001375:Azaguanine