Activation of particle associated rat liver guanine deaminase by lecithin and interferences of lecithin in protein precipitation.
Guanine deaminase solubilized from the "light" mitochondrial fraction of rat liver was activated by lecithin. The activation was proportional to the concentration of lecithin taken in the system. A ratio of 1:1 between the two constituents (protein and lecithin) was at least necessary for complete precipitation.
D000619:Aminohydrolases / Q000378:metabolism*; D000818:Animals; D011232:Chemical Precipitation; D006148:Guanine Deaminase / Q000302:isolation & purification / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000494:pharmacology*; D011489:Protein Denaturation / Q000187:drug effects; D051381:Rats
D010713:Phosphatidylcholines; D000619:Aminohydrolases; D006148:Guanine Deaminase