title: Polypeptides of mumps virus.
abstract: Mumps virus was propagated in the extra-embryonic fluids of embryonated chicken eggs and was labeled by cionjection of radioactively labeled amino acids. The virus was purified by density gradient centrifugation, and its polypeptides were analyzed by polyarylamide gel electrophoresis. The virus was found to be composed of six polypeptides, ranging in size from 40,000 to 64,000 daltons. Viral proteins 1 and 3 were the glycoproteins of the virons. When the virus particle was treated with noniontic detergents, a small fraction of these glycoproteins could be released into the supernatant. After treatment with nonionic detergents in high salt and alkaline conditions, more of the surface glycoproteins were removed. This treatment also released the smallest viral polypeptide from the virion. The glycoproteins were separated using an affinity chromatographic column of agarose-fetuin. The heavier glycoprotein, viral protein 1, was found to contain both the neuraminidase and hemagglutinating activity. The two glycoproteins were tested for their ability to react in complement-fixing tests with mumps antisera. Only the heavier glycoprotein reacted with antisera possessing both anti-S and anti-V activity. Neither glycoprotein reacted with antisera specific for the S antigen. Thus, it was concluded that this glycoprotein corresponds to the classical V antigen of mumps virus.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002642:Chick Embryo; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000032:analysis; D009109:Mumps virus / Q000032:analysis* / Q000187:drug effects / Q000276:immunology; D009439:Neuraminidase / Q000032:analysis; D011092:Polyethylene Glycols / Q000494:pharmacology; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D014764:Viral Proteins; D011092:Polyethylene Glycols; D009439:Neuraminidase