[Molecular parameters of the sensitization of tannined erythrocytes with human IgG].
A study was made of the molecular binding parameters during the sensitization of human IgG with tannin-treated sheep erythrocytes, depending on the concentration of the reacting components. The total amount of IgG molecules stably bound by all erythrocytes increased with the elevation of erythrocyte and IgG concentration. About 1.7 million IgG molecules were stably fixed on one erythrocyte under conditions providing the maximum binding. However, the level of hemosensitization approaching the maximum was provided by binding 400,000--600,000 IgG molecules by one erythrocyte. Apparently the level of specific IgG sensitization was determined not only by the amount of protein molecules stably bound by one erythrocyte, but also by the character of space position of the IgG molecules on the erythrocyte surface.
D001667:Binding, Competitive; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000302:isolation & purification; D008968:Molecular Conformation; D009500:Neutralization Tests; D013634:Tannins / Q000276:immunology*
D007074:Immunoglobulin G; D013634:Tannins
Molekuliarnye parametry sensibilizatsii tanizirovannykh éritrotsitov IgG cheloveka