The combined effect of nonsteroidal anti-oestrogens and sex steroids on the growth of rat uterine adenocarcinoma cells in tissue culture.
Nonsteroidal anti-oestrogenic drugs, tamoxifen and clomiphene citrate, at concentrations higher than 0.001 mug/ml reduced the colony forming ability of cells derived from a rat uterine adenocarcinoma in vitro. The 50 per cent inhibitory dose of these drugs was about one-hundredth of that of sex steroids. When the cells were treated with combinations of these nonsteroidal anti-oestrogenic drugs and the 50 per cent inhibitory dose (8 mug/ml) of progesterone, a synergistic effect on the inhibition of colony formation was observed. In contrast to progesterone, oestradiol-17beta (the 50 per cent inhibitory dose of which was about 16 mug/ml) suppressed additively the colony formation only in combination with low doses of anti-oestrogenic drugs.
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002996:Clomiphene / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D013267:Stilbenes / Q000494:pharmacology*; D013629:Tamoxifen / Q000494:pharmacology*; D014594:Uterine Neoplasms / Q000473:pathology*; D014599:Uterus / Q000473:pathology*
D013267:Stilbenes; D013629:Tamoxifen; D002996:Clomiphene; D011374:Progesterone; D004958:Estradiol