New derivatives for the analysis of sphingosine long-chain bases by gas-liquid chromatography-mass spectrometry.
Cyclic derivatives formed by reaction of sphingosines with methane-, n-butane- and benzeneboronic acid have been examined by gas-liquid chromatography (GLC) and by combined GLC-mass spectrometry (MS). Analogous compounds have also been studied, in which the amino group has been protected, for example as an acetone Schiff base or N,N-dimethylaminomethylene derivative. Phytosphingosine (4D-hydroxysphinganine) yielded bis-alkaneboronates. Most of the derivatives were satisfactory for GLC. Molecular ions were apparent (1-100% relative abundance) in all electron impact mass spectra, and and (as [M+1](+)ions) were the base peaks in isobutane chemical ionisation mass spectra.
D001158:Arteries / Q000032:analysis; D001896:Boron Compounds / Q000032:analysis; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013058:Mass Spectrometry; D012545:Schiff Bases; D013107:Sphingolipids / Q000032:analysis; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000032:analysis
D001896:Boron Compounds; D012545:Schiff Bases; D013107:Sphingolipids; D013110:Sphingosine