Mutagenic effect of sodium nitrite on cultured mouse cells.
Effect of sodium nitrite on cultured FM3A cells, a C3H mouse mammary carcinoma cell line, was examined. The chromosomal preparations demonstrated that severe aberrations were induced in more than 80% of the mitotic plates at 10(-2) M and in nearly 40% at 10(-25) M after 24 and 48 h treatment. According to the results of alkaline sucrose gradient analysis sedimentation profiles of cell DNA treated at as high as 10(-1) M for 24 h scarcely changed from that of control cell DNA. Induction of 8-azaguanine-resistant mutation was demonstrated above 10(-3) M sodium nitrite.
D000818:Animals; D001375:Azaguanine / Q000494:pharmacology; D002277:Carcinoma; D002460:Cell Line; D002470:Cell Survival; D002869:Chromosome Aberrations; D004277:DNA, Single-Stranded; D004305:Dose-Response Relationship, Drug; D008321:Mammary Glands, Animal; D051379:Mice; D008809:Mice, Inbred C3H; D008938:Mitosis; D009154:Mutation; D009573:Nitrites / Q000494:pharmacology*; D013997:Time Factors
D004277:DNA, Single-Stranded; D009573:Nitrites; D001375:Azaguanine