Methylation and acetolysis of extracellular D-mannans from yeast.
Methylation-fragmentation analyses were conducted on a series of extra-cellular, yeast alpha-D-linked mannans representing six different structural types. D-Mannans of low degree of branching were produced by Hansenula capsulata strains and by species related to H. holstii, The former consisted primarily of (1 leads to 2)- and (1 leads to 6)-linked D-mannosyl residues; the latter, of (1 leads to 2)- and (1 leads to 3)-linked D-mannosyl residues. Although the remaining structural types were highly branched, each gave distinct methylation-patterns indicative of (1 leads to 6)-linked backbones to which are appended non-(1 leads to 6)-linked side-chains. Acetolysis studies were correlated with the methylation analyses, and the correlation demonstrated that each branched polymer possesses side chains of heterogeneous length.
D000107:Acetylation; D001203:Ascomycota / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D008351:Mannans; D008745:Methylation; D010843:Pichia / Q000032:analysis*; D011134:Polysaccharides; D013045:Species Specificity; D013329:Structure-Activity Relationship; D015003:Yeasts / Q000032:analysis*
D008351:Mannans; D011134:Polysaccharides