Characterization of miniature pig kidney cells and their resistance to chlamydial infection.
A miniature pig kidney cell line has been established from porcine fetuses taken aseptically by hysterectomy and maintained for more than 50 passages in Eagle's minimal essential medium containing 10% heat-inactivated newborn calf serum. Cell transfers were performed each week. Primary and serially passaged cells were found to be highly refractory to infection by Chlamydia trachomatis strains TW-3, Bour, and LGV 440L and Chlamydia psittaci strains meningopneumonitis and 6BC and insusceptible to poliovirus type 1. The cells were susceptible to vesicular stomatitis virus and herpes simplex type 1 virus. Identity of the cells was established by cytotoxicity, isozyme, and cytogenetic studies.
D000818:Animals; D002460:Cell Line; D002689:Chlamydia / Q000254:growth & development*; D002692:Chlamydia trachomatis / Q000254:growth & development; D002691:Chlamydophila psittaci / Q000254:growth & development; D007527:Isoenzymes; D007668:Kidney; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D013552:Swine
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase