Rapid screening of lipid metabolism in monolayer cell cultures.
Monolayer cell cultures grown on coverslips in the presence of radioactive lipid precursors were embedded in silica gel layers for extraction and resolution of the labelled products directly by thin-layer chromatography. The method permits rapid screening of lipid metabolism in tissue cultures with a small number of cells.
D002250:Carbon Radioisotopes; D002460:Cell Line; D002855:Chromatography, Thin Layer; D005233:Fatty Alcohols / Q000378:metabolism; D050356:Lipid Metabolism; D010169:Palmitic Acids / Q000378:metabolism; D014316:Tritium
D002250:Carbon Radioisotopes; D005233:Fatty Alcohols; D010169:Palmitic Acids; D014316:Tritium