Hemoglobin synthesis in cell hybrids formed between teratocarcinoma and Friend erythroleukemia cells.
Viable hybrid cells have been isolated following fusion of Friend erythroleukemia cells and undifferentiated teratocarcinoma cells. The hybrids formed between near-diploid parental cells resembled Friend cells in the ability to grow in suspension and to synthesize hemoglobin in the presence of the chemical inducers dimethyl sulfoxide (DMSO) and ouabain. Erythropoietin (EPO) was effective in inducing hemoglobin synthesis in some of the hybrid cell lines. The hemoglobins synthesized by the hybrids were of the adult forms, but were quantitatively different from those hemoglobins synthesized by the parental Friend cells, suggesting that the fusion event modulated the expression of the hemoglobin chain genes.
D000818:Animals; D002454:Cell Differentiation; D002459:Cell Fusion; D002460:Cell Line; D002875:Chromosomes / Q000032:analysis; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004921:Erythropoietin / Q000494:pharmacology; D005622:Friend murine leukemia virus; D006454:Hemoglobins / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis / Q000378:metabolism*; D007942:Leukemia, Experimental; D051379:Mice; D009368:Neoplasm Transplantation; D010042:Ouabain / Q000494:pharmacology; D013724:Teratoma
D006454:Hemoglobins; D004921:Erythropoietin; D010042:Ouabain; D004121:Dimethyl Sulfoxide