A simple method for the preparation of [beta-32P]purine nucleoside triphosphase.
A rapid, simple and inexpensive procedure is described for the preparation of purine ribo-and deoxyribonucleoside triphosphates specifically and highly radiolabeled with [32P]phosphate in the beta position. The method involves two successive enzymatic reactions: conversion of donor [gamma-32P]ATP in the presence of an excess of acceptor 5'-mononucleotide to the 5'-diphosphates by myokinase or guanosine 5'-monophosphate kinase followed by phosphorylation with pyruvate kinase to yield 5'-triphosphates.
D000263:Adenylate Kinase; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D010761:Phosphorus Radioisotopes; D011685:Purine Nucleotides / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012087:Reoviridae / Q000201:enzymology; D014616:Vaccinia virus / Q000201:enzymology
D010761:Phosphorus Radioisotopes; D011685:Purine Nucleotides; D012333:RNA, Messenger; D000263:Adenylate Kinase; D012321:DNA-Directed RNA Polymerases; D010729:Phosphoenolpyruvate Carboxykinase (GTP)