Use of a low-ionic-strength medium in manual tests for antibody detection.
The present observations confirm the value of suspending red blood cells in a low-ionic-strength medium in the first stage of the indirect antiglobulin test; that is, during the period of incubation with antibody-containing serum. The main advantage of this procedure is to shorten the time of incubation. In this respect a low-ionic-strength medium appears to be superior to albumin as a suspending medium for the red blood cells. A further advantage is to increase the uptake of certain antibodies; this effect was pronounced with selected Rh antibodies believed to be of low affinity.
D002021:Buffers; D003298:Coombs Test; D005189:False Positive Reactions; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G; D007518:Isoantibodies / Q000032:analysis*; D009994:Osmolar Concentration; D012965:Sodium Chloride; D012996:Solutions; D013997:Time Factors
D002021:Buffers; D007106:Immune Sera; D007074:Immunoglobulin G; D007518:Isoantibodies; D012996:Solutions; D012965:Sodium Chloride