Metabolism of L-(U-14C)valine, L-(U-14C)leucine, L-(U-14C)histidine and L-(U-14C) phenylalanine by the isolated perfused lactating guinea-pig mammary gland.
1. The incorporation of L-[U-14C]leucine, L[U-14C]histidine and L-[U-14C]phenylalanine into casein secreted during perfusion of isolated guinea-pig mammary glands was demonstrated. 2. The extent of incorporation of label into casein residues was consistent with their being derived from free amino acids of the perfusate plasma. 3. The mean transit time of the amino acids from perfusate into secreted casein was approx. 100 min. 4. Whereas radioactive histidine and phenylalanine were incorporated solely into milk protein, radioactivity from [U-14C]valine was also transferred to CO2 and to an unidentified plasma component, and from [U-14C]leucine to plasma glutamic acid. 5. Evidence from experiments with [U-14C]phenylalanine suggests that, as in rats, but in contrast with ruminant species, guinea-pig mammary tissue does not possess phenyl alanine hydroxylase activity. 6. The results are discussed in relation to the possible role of essential amino acid catabolism in the control of milk-protein synthesis.
D000601:Amino Acids, Essential / Q000378:metabolism*; D000818:Animals; D002364:Caseins / Q000032:analysis; D005260:Female; D005971:Glutamates / Q000097:blood; D006168:Guinea Pigs; D006639:Histidine / Q000378:metabolism; D066298:In Vitro Techniques; D007774:Lactation; D007930:Leucine / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism*; D010477:Perfusion; D010649:Phenylalanine / Q000378:metabolism; D011247:Pregnancy; D014443:Tyrosine / Q000097:blood; D014633:Valine / Q000378:metabolism
D000601:Amino Acids, Essential; D002364:Caseins; D005971:Glutamates; D014443:Tyrosine; D010649:Phenylalanine; D006639:Histidine; D007930:Leucine; D014633:Valine