Radioimmunoassay of sisomicin.
An antibody induced in rabbits against a gentamicin-bovine serum albumin conjugate cross-reacted with the chemically related aminoglycoside, sisomicin, facilitating the development of a sisomicin radioimmunoassay. Sisomicin was labeled with (125)I using an acylating agent [3-(4-hydroxyphenyl)propionic acid-N-hydroxy-succinimide ester]. The resulting assay produced a linear standard curve on a logit-log plot with a sensitivity of 140 pg. Comparison with a microbiological assay showed no significant difference (P < 0.001) in the measurement of sisomicin by these two methods.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D003429:Cross Reactions; D005839:Gentamicins / Q000276:immunology; D007114:Immunization; D007457:Iodine Radioisotopes; D008722:Methods; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine; D012853:Sisomicin / Q000032:analysis*
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine; D012853:Sisomicin