Urinary 3-methoxy-4-hydroxyphenylacetic (homovanillic) and 3-methoxy-4-hydroxymandelic (vanillylmandelic) acids: gas-liquid chromatographic methods and experience with 13 cases of neuroblastoma.
We present a quantitative gas-chromatographic method for determining urinary 3-methoxy-4-hydroxymandelic acid (vanillylmandelic acid) and 3-methoxy-4-hydroxyphenylacetic acid (homovanillic acid). In this rapid technique an internal standard is used and the procedure involves ether extraction and silyl ether formation. Analytical recovery of vanillylmandelic acid averages 87.5% (CV, 0.95%), of homovanillic acid 102.3% (CV, 9.95%). Our data on 34 samples from 13 neuroblastoma patients show that homovanillic acid is more consistently elevated than is vanillylmandelic acid.
D002648:Child; D002849:Chromatography, Gas / Q000379:methods; D006719:Homovanillic Acid / Q000652:urine*; D006801:Humans; D009447:Neuroblastoma / Q000652:urine*; D010648:Phenylacetates / Q000652:urine*; D014642:Vanilmandelic Acid / Q000652:urine*
D010648:Phenylacetates; D014642:Vanilmandelic Acid; D006719:Homovanillic Acid