An improved preparation for determination of bradykinin.
The cat isolated jejunum kept either in an organ-bath or under superfusion can be rendered extremely sensitive to bradykinin after exposure to chymotrypsin. Treated preparations responded to as little as 100 pg/ml of the peptide. Use has been made of sensitized preparations to determine bradykinin-like material of dog blood extracts obtained in experimental "dumping syndrome".
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000032:analysis* / Q000097:blood / Q000494:pharmacology; D002415:Cats; D002918:Chymotrypsin; D005260:Female; D066298:In Vitro Techniques; D007583:Jejunum / Q000187:drug effects / Q000502:physiology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D012701:Serotonin / Q000494:pharmacology
D012701:Serotonin; D002918:Chymotrypsin; D000109:Acetylcholine; D001920:Bradykinin