title: Regional differences between nuclear concentration of (3H) estradiol and (3H) progesterone and their action on the uterus of rat during delayed implantation.
abstract: The cellular and subcellular localization of radioactivity by dry-mount autoradiography was studied at 5, 15 and 30 minutes after intraluminal or at 5, 15, and 60 minutes after subcutaneous administration of (3H) estradiol or (3H) progesterone to rats in which implantation was delayed by ovariectomy on day 3 post-coitum, followed by treatment with progesterone. After intraluminal instillation of (3H) estradiol or (3H) progesterone, radioactivity was cleared from the uterine lumen at 5 minutes. After intraluminal as well as subcutaneous injection of (3H) estradiol, radioactivity was concentrated at all time intervals in the nuclei of cells of the substantia propria, glands and muscularis, but not in the luminal epithelium. White blood cells and endothelial cells were unlabeled, while perivascular cells showed concentration of radioactivity. After intraluminal instillation of (3H) progesterone, radioactivity was retained in the cytoplasm of the luminal epithelium at 5 and 15 minutes, while no such concentration appeared after subcutaneous injection. No selective nuclear retention was observed in the luminal epithelium, in contrast to the substantia propria and muscularis. The autoradiographic results indicate that progesterone treatment simulating the conditions of early pregnancy, changes the uptake affinity of uterine tissues for (3H) estradiol. The lack of a detectable concentration of estrogen within nuclei of the luminal epithelium does not preclude hormone action. Possible mechanisms of metabolic activation of the uterine luminal epithelium in the apparent absence of concentration of (3H) estradiol to nuclei of the luminal epithelium are discussed.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D010064:Embryo Implantation; D010065:Embryo Implantation, Delayed; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D011374:Progesterone / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D014599:Uterus / Q000378:metabolism*
chemical_list: D011374:Progesterone; D004958:Estradiol