title: Procedural difficulties in the gas-liquid chromatographic assay of the arylalkylamines.
abstract: A gas-liquid chromatographic method for the estimation of 2-phenylethylamine in rat brain tissue is described. The procedure involves initial separation of the amine from homogenates with ion-exchange chromatography, subsequent acetylation of the eluate and extraction of the N-acetylated amine with ethyl acetate. The N-acetyl-2-phenylethylamine derivative is then converted to the pentafluoropropionyl derivative and quantitated with electron capture detection. Difficulties involved with alternative separative procedures and derivative formation are discussed.
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002849:Chromatography, Gas / Q000379:methods*; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D010627:Phenethylamines / Q000032:analysis*; D051381:Rats
chemical_list: D010627:Phenethylamines