Platelet adhesion to solid surfaces. The effects of plasma proteins and substrate wettability.
Platelet adhesion tests were performed using protein-free washed pig platelet suspensions in conjunction with a simple open-static method on smooth, well-defined protein-coated glass and polymer surfaces and bare glass and polymer surfaces. A normalization technique was introduced in an attempt to correct day-to-day variations in platelet reactivity. Protein coatings reduced platelet adhesion (to glass as well as polymer surfaces) to such a low level that the platelet density on one protein/substrate combination could not be distinguished from that on any other protein/substrate combination. Specifically, albumin and fibrinogen behaved identically regardless of the substrate on which they were coated. The addition of albumin and gamma-globulin to the platelet-suspension also significantly reduced platelet adhesion to glass surfaces. Whereas the extent of platelet adhesion from protein-free suspensions to bare glass and polymer surfaces depended on their wettability, the presence of specific chemical groups in the proteins, such as (-CONH-), may have an overriding effect on platelet adhesion to solid surfaces.
D001798:Blood Proteins; D005898:Glass; D006358:Hot Temperature; D010973:Platelet Adhesiveness; D011108:Polymers; D011137:Polystyrenes; D012821:Silanes; D012828:Silicones; D013499:Surface Properties; D013500:Surface Tension
D001798:Blood Proteins; D011108:Polymers; D011137:Polystyrenes; D012821:Silanes; D012828:Silicones