Micromethod for analysis for chloride in physiological fluids.
A micromethod for the analysis for chloride, based on the chemical precipitation of silver chloride by radial diffusion through agar gel containing silver nitrate, is described. The method is simple to run, requires little or no instrumentation, and requires only 10 mul of sample. Results by coulometric titration (Buchler Cotlove Chloridometer) correlated well for serum (r = 0.961), urine (r = 0.997), cerebrospinal fluid (r = 0.991), and sweat (r = 0.998). Other halide ions or protein do not interfere. Precision studies gave a within-day reproducibility (CV) of 1.3% and a day-to-day variability of 2.1% for a serum sample averaging 115 mmol/liter.
D000362:Agar; D002712:Chlorides / Q000032:analysis* / Q000097:blood; D004058:Diffusion; D005069:Evaluation Studies as Topic; D006801:Humans; D007454:Iodides; D008722:Methods; D008832:Microchemistry; D012835:Silver Nitrate; D013542:Sweat / Q000032:analysis
D002712:Chlorides; D007454:Iodides; D000362:Agar; D012835:Silver Nitrate