Drug-resistant mutants of Chinese hamster ovary cells possess an altered cell surface carbohydrate component.
Surface label experiments using the galactose oxidase-]3H]-borohydride technique reveal that cells from drug-resistant Chinese hamster ovary clones possess a surface carbohydrate component of apparent molecular weight 165,000 which is absent from wild-type cells. The component may also be demonstrated by [14C]glucosamine incorporation but not by [3H]leucine incorporation or by the lactoperoxidase surface labeling reaction.
D001665:Binding Sites; D001894:Borohydrides; D050260:Carbohydrate Metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004351:Drug Resistance; D005944:Glucosamine / Q000378:metabolism; D007784:Lactoperoxidase / Q000378:metabolism; D008970:Molecular Weight; D009154:Mutation; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism
D001894:Borohydrides; D011506:Proteins; D007784:Lactoperoxidase; D005944:Glucosamine