Affinity chromatography of soluble galactosyltransferases using an easily synthesized N-acetylglucosamine-agarose bead.
Agarose derivatized wtih phi-nitrophenyl-N-acetyl-beta-d-glucosamine was used for affinity chromatography for soluble galactosyltransferase from various sources. Moreover, this adsorbant acts as an acceptor for galactose when incubated with prepurified galactosyltransferase.
D000117:Acetylglucosamine; D002846:Chromatography, Affinity / Q000379:methods; D005690:Galactose / Q000302:isolation & purification; D005700:Galactosyltransferases / Q000097:blood* / Q000302:isolation & purification*; D006801:Humans; D009596:Nitrophenols; D012685:Sepharose
D009596:Nitrophenols; D012685:Sepharose; D005700:Galactosyltransferases; D000117:Acetylglucosamine; D005690:Galactose