title: Viscometric assay of bacterial alginase.
abstract: Equations defining the reaction of microbial alginase on commercial sodium alginate are presented with respect to the viscometric assay of enzyme activity. The negative log of K was found to be linearly related to the concentration of algin, where K is defined as the change in the reciprocal of relative viscosity with time. The negative log of K was also found to be linearly related to the amount of enzyme in reaction mixtures when the substrate concentration was held constant.
mesh_terms: D000464:Alginates / Q000378:metabolism; D002474:Cell-Free System; D006026:Glycoside Hydrolases / Q000378:metabolism*; D014737:Vibrionaceae / Q000201:enzymology*; D014783:Viscosity; D014871:Water Microbiology
chemical_list: D000464:Alginates; D006026:Glycoside Hydrolases