Effects of prednisolone metasulfabenzoate on the induction of DNase II in comparison to alkaline phosphatase and acid phosphatase activities in cultures of HeLa S3 cells.
Cultures of HeLa S3 cells were treated with prednisolone metasulfobenzoate (Na), a derivative of prednisolone which is readily soluble in water. The steroid induced an increase in DNase II, a lysosomal enzyme which was not used previously in enzyme induction by steroids. Alkaline phosphatase, a known inducible enzyme by other steroids and acid phosphatase, a known uninducible enzyme by other steroids, were included for comparative reasons.
D000135:Acid Phosphatase / Q000096:biosynthesis*; D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D003851:Deoxyribonucleases / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D006367:HeLa Cells / Q000201:enzymology; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D012995:Solubility; D013997:Time Factors
C013218:prednisolone 21-3-sulfobenzoate; D011239:Prednisolone; D003851:Deoxyribonucleases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase