Colorimetric determination of aliphatic acids.
A colorimetric method for the determination of carboxylic acids based on the dicyclohexylcarbodiimide-coupled reaction of 2-nitrophenylhydrazine and carboxylic acids is described. The product of the reaction is extracted into aqueous sodium hydroxide to produce a blue color. This method is suitable for the analysis of aliphatic acids, but aromatic acids do not react under these conditions.
D000085:Acetates; D002264:Carboxylic Acids / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D004024:Dicyclohexylcarbodiimide; D006834:Hydrazines; D007202:Indicators and Reagents; D007700:Kinetics; D011725:Pyridines
D000085:Acetates; D002264:Carboxylic Acids; D006834:Hydrazines; D007202:Indicators and Reagents; D011725:Pyridines; D004024:Dicyclohexylcarbodiimide