The effect of bovine serum albumin on the in vitro inhibition of chemotaxis by anti-inflammatory agents.
The anti-inflammatory agents, phenylbutazone, naproxen and niflumic acid inhibited in vitro rabbit peritoneal neutrophil chemotactic responsiveness to Escherichia coli derived chemotactic factor/s when added to cells suspended in 0.1% bovine serum albumin (BSA). Inhibition of chemotaxis by these drugs was markedly diminished as the BSA concentration was increased to 2%. Experiments with phenylbutazone demonstrated that inhibition of chemotaxis could be observed using neutrophils suspended in 2% BSA by either increasing the drug concentration or by preincubating the cells suspended in 0.1% with the drug prior to the addition of the additional BSA. These data suggest that protein binding can prevent in vitro inhibition of neutrophil chemotaxis by anti-inflammatory agents.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002417:Cattle; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004347:Drug Interactions; D005260:Female; D066298:In Vitro Techniques; D009288:Naproxen / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects; D009544:Niflumic Acid / Q000494:pharmacology; D010653:Phenylbutazone / Q000494:pharmacology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D012710:Serum Albumin, Bovine; D009544:Niflumic Acid; D009288:Naproxen; D010653:Phenylbutazone