[Distribution and mechanism of the carcinogenic action of 1,2-dimethylhydrazine in rats].
The radioactivity of blood, bile, urine and contents of isolated intestinal segments was measured at various periods after 3H-1,2-dimethylhydrazine (3H-DMH) and 3H-1,2-diethylhydrazine (3H-DEH) subcutaneous administration. These experiments have revealed that DMH metabolites entered the intestine both with bile and directly through the intestinal wall. The DMH metabolites entering the bowel as glucuronides are cleaved with beta-glucuronidase of bacterial origin, and as a result of this the alkylation of enterocytes nucleic acids and protein takes place. In totally hepatectomized animals no methylation of enterocyte macromolecules was noted. DEH fails to penetrate the intestinal wall and to alkylate biomolecules of enterocytes, when administered parenterally or per os. Some possible DMH metabolic pathways are discussed, and the dynamics of DNA and protein methylation is analysed in the light of these proposed pathways.
D000478:Alkylation; D000818:Animals; D001646:Bile / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004064:Digestive System / Q000378:metabolism*; D004127:Dimethylhydrazines / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism; D006498:Hepatectomy; D006834:Hydrazines / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D012313:RNA / Q000378:metabolism; D051381:Rats
D002273:Carcinogens; D004127:Dimethylhydrazines; D006834:Hydrazines; D012313:RNA; D004247:DNA; D005966:Glucuronidase
Raspredelenie i mekhanizm kantserogennogo deĭstviia 1,2-dimetilgidrazina u krys