The use of aldehyde dehydrogenase to determine H2O2 -producing reactions. I. The determination of the uric acid concentration.
The sequence of reactions catalyzed by uricase, catalase and aldehyde dehydrogenase for determination of the uric acid concentration was evaluated for human serum and urine samples. More than 60 substances were tested for possible interferences. Alcohol dehydrogenase, formaldehyde and homogentistic acid can disturb the proposed assay, but at concentrations which are not usually encountered in human serum or urine. In the presence of protein at least 99% of the uric acid was recovered.
D000429:Alcohol Oxidoreductases / Q000097:blood; D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors; D001322:Autoanalysis / Q000379:methods; D003404:Creatinine / Q000097:blood; D003951:Diagnostic Errors; D006713:Homogentisic Acid / Q000652:urine; D006801:Humans; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D014527:Uric Acid / Q000032:analysis*
D007202:Indicators and Reagents; D014527:Uric Acid; D003404:Creatinine; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; D000445:Aldehyde Oxidoreductases; D006713:Homogentisic Acid