Radiation-induced DNA scissions and their rejoining in testicular cells of mouse.
The radiosensitivity of DNA molecules in testicular cells from adult male mice in situ was 0.22 single-strand breks per 10(12) daltons of DNA per rad. This was significantly lower than the 0.6-0.7 for liver and thymus DNA, and was most likely attributable to a hypoxic condition of testicular cells in situ. Although a majority of the single-strand scissions was quickly rejoined, a significant extent of breaks remained without further rejoining. The presence of remaining breaks was demonstrated not only after 3-6 h of post-irradiation time, but also after irradiation at low dose rates. The remaining breaks might be a slow and/or non-reparable DNA damage.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002869:Chromosome Aberrations; D004247:DNA / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D008297:Male; D051379:Mice; D011831:Radiation Genetics; D013737:Testis / Q000528:radiation effects*; D013997:Time Factors
D004247:DNA