title: Titration of Newcastle disease virus and its neutralizing antibodies in microplates by a modified hemadsorption and hemadsorption inhibition method.
abstract: Using the microtiter system, titration of Newcastle disease virus infectivity and neutralizing antibodies was carried out in chicken embryo fibroblasts grown in "U" or flat-bottomed plates. Infectivity was detected by a combined hemadsorption-hemagglutination method. Inhibition of that reaction indicated the presence of neutralizing antibodies. A 24-h microneutralization test was developed and compared to the plaque neutralization and microhemagglutination inhibition test. Reproducibility of the microneutralization test was statistically analyzed.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002642:Chick Embryo; D046508:Culture Techniques / Q000379:methods; D006382:Hemadsorption; D006383:Hemadsorption Inhibition Tests; D006385:Hemagglutination Inhibition Tests; D009500:Neutralization Tests; D009522:Newcastle disease virus / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral