Development of a microassay for estradiol receptors.
The calculation of equilibrium binding constants for a specific receptor-hormone interaction requires the exact determination of the unbound ligand concentration and the specifically bound concentration at equilibrium. These determinations usually require corrections for the contribution of non-specific binding. We introduce several parameters allowing equilibrium concentrations to be calculated from experimental concentration values; these parameters can be measured for the particular receptor assay procedure used. These parameters are used in a microassay of estradiol-receptor complex by selective retention on DE-81 cellulose paper.
D000818:Animals; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008433:Mathematics; D008722:Methods; D008832:Microchemistry; D011960:Receptors, Estrogen / Q000378:metabolism*; D012756:Sheep; D014599:Uterus / Q000378:metabolism
D011960:Receptors, Estrogen; D004958:Estradiol