title: Demonstration of concanavalin A receptors on Leptomonas pessoai cell memebrane.
abstract: Leptomonas pessoai promastigotes cultivated in a synthetic medium were agglutinated with concanavalin A (ConA). Agglutination was predominantly of the flagellar-flagellar type, and was inhibited with sucrose or alpha-methyl-D-mannoside. Cell surface polysaccharides and/or glycoproteins were demonstrated by several cytochemical methods at the fine-structural level. A Con A-horseradish peroxidase-diaminobenzidine (DAB) technic was used to detect Con A receptors in the pellicular membrane of the flagellar pocket region and in the flagellar membrane proper. Somewhat less of the Con A-DAB reaction product was observed in the pellicular membrane enclosing the rest of the cell.
mesh_terms: D000371:Agglutination; D000818:Animals; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D003208:Concanavalin A / Q000494:pharmacology*; D056890:Eukaryota / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D003208:Concanavalin A