Carbodimide coupling to formalin-fixed red blood cells. A direct passive hemagglutination test for anti-horse ferritin.
The water-soluble carbodiimide, 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate, has been used to couple horse ferritin covalently to formalin-fixed, human red blood cells. This method has been optimized with respect to sensitivity, range, and reproducibility. The sensitized cells are stable for at least 6 months at 4 degrees C, and they may be stored frozen without additives or special precautions. Titers of 1/216 are routinely obtained with antisera to ferritin.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001793:Blood Preservation; D002234:Carbodiimides / Q000378:metabolism* / Q000494:pharmacology; D002352:Carrier Proteins / Q000276:immunology*; D004912:Erythrocytes / Q000378:metabolism*; D005293:Ferritins / Q000276:immunology* / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D006386:Hemagglutination Tests; D006736:Horses; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D011817:Rabbits; D012965:Sodium Chloride; D012996:Solutions; D013997:Time Factors
D000906:Antibodies; D002234:Carbodiimides; D002352:Carrier Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012996:Solutions; D005557:Formaldehyde; D012965:Sodium Chloride; D005293:Ferritins