title: Biosynthesis of proteins by the retina. Inactivation by near-ultraviolet light and the effects of tryptophan, epinephrine, and catalase.
abstract: Postmitochondrial supernatant, cytosol, and microsomes were exposed to near-ultraviolet light and assayed for incorporation of (3H)leucine. The results indicate that near-ultraviolet light decreased the incorporation of radioactivity into the aminoacyl-tRNA fraction and into proteins. This effect depended on the wavelength of irradiation and the concentration of exposed protein. Tryptophan and epinephrine potentiated the effect of near-ultraviolet light, but catalase prevented this effect. Hydrogen peroxide decreased protein synthesis when it was added to unirradiated postmitochondrial supernatant. The results suggest that near-ultraviolet light would decrease protein synthesis by generating hydrogen peroxide from tryptophan and epinephrine.
mesh_terms: D000418:Albumins / Q000494:pharmacology; D000818:Animals; D002374:Catalase / Q000494:pharmacology*; D002474:Cell-Free System; D004837:Epinephrine / Q000494:pharmacology*; D005136:Eye Proteins / Q000096:biosynthesis*; D005260:Female; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D008297:Male; D012343:RNA, Transfer / Q000378:metabolism; D051381:Rats; D012160:Retina / Q000378:metabolism* / Q000528:radiation effects; D014364:Tryptophan / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D000418:Albumins; D005136:Eye Proteins; D014364:Tryptophan; D012343:RNA, Transfer; D006861:Hydrogen Peroxide; D002374:Catalase; D007930:Leucine; D004837:Epinephrine