A genetic approach to studies on the synthesis of the complementary RNA's of fowl plague virus.
The rate of incorporation of 5-fluorouracil into complementary strands of replicating RNA of fowl plague virus (FPV) has been studied. The efficiency of incorporation was estimated by determination of the reversion frequency in s-mutants with known types of base transitions in the RNA of the virus particle. It was established that maximum incorporation of 5-fluorouracil into progeny virus particle RNA took place between 2 and 4 h after infection. The maximum incorporation of the mutagen into complementary RNA (plus strands) occurred when the cells were exposed to 5-fluorouracil from 1 to 2 h after infection.
D001483:Base Sequence; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005472:Fluorouracil / Q000378:metabolism / Q000494:pharmacology; D006898:Hydroxylamines / Q000494:pharmacology; D009980:Influenza A virus / Q000254:growth & development / Q000378:metabolism*; D009153:Mutagens; D009154:Mutation; D009608:Nitrous Acid / Q000494:pharmacology; D012367:RNA, Viral / Q000096:biosynthesis*; D014779:Virus Replication
D006898:Hydroxylamines; D009153:Mutagens; D012367:RNA, Viral; D005020:Ethyl Methanesulfonate; D009608:Nitrous Acid; D005472:Fluorouracil