Plasma volume measurements in sheep using Evans' blue and continuous blood sampling.
After intravenous injection of 0.3-0.4 mg Evans' blue/kg liveweight in sheep, blood was collected continuously for 10 min into 10 tubes. Calculations using the dye concentrations in the plasma collected between 3 and 10 min gave values of plasma volume in close agreement with those obtained by intravascular dilution of labelled macromolecules. The dye has no adverse effects on carcase quality and can be used in standard holding pens.
D000818:Animals; D001391:Azo Compounds; D001800:Blood Specimen Collection / Q000662:veterinary*; D005070:Evans Blue; D005260:Female; D010953:Plasma Volume; D012756:Sheep / Q000502:physiology*
D001391:Azo Compounds; D005070:Evans Blue