Colorimetric determination of aconitine in galenicals and in biological samples.
A sensitive method is presented for determining aconitine. Aconitine is complexed with Co2+, the aconitine-cobalt complex is extracted with chloroform, and the absorbance is measured at 320 nm. The sensitivity of the method ranged between 0.06 and 3 mg/25 ml, and the color was stable for 6 hr. The method was successfully applied for the quantitative determination of aconitine in animal tissues.
D000157:Aconitine / Q000032:analysis* / Q000097:blood / Q000652:urine; D031841:Aconitum / Q000031:analogs & derivatives*; D002614:Chelating Agents; D003035:Cobalt; D003124:Colorimetry / Q000379:methods; D004338:Drug Combinations; D008099:Liver / Q000032:analysis; D008832:Microchemistry; D009206:Myocardium / Q000032:analysis; D012996:Solutions / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013270:Stomach / Q000032:analysis
D002614:Chelating Agents; D004338:Drug Combinations; D012996:Solutions; D003035:Cobalt; D000157:Aconitine