The effects of prostaglandin E-1 and F-2 alpha on maturation of mouse oocytes in vitro.
The direct effects of prostaglandin E-1 (PGE-1) and F-2 alpha (PGF-2alpha) on isolated mouse oocytes were examined in a microtube culture method. In oocytes exposed to PGE-1, meiotic resumption was suppressed at the anaphase I stage, presenting an irregular arrangement of the chromosomes along with the spindle fibres which were characterized by nondisjunction of the tetrads. PGF-2alpha was less potent in suppressing oocyte maturation than PGE-1. The critical time of the action of PGE-1 was between 7-5 and 12 hr of incubation.
D000818:Animals; D002875:Chromosomes / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D009865:Oocytes / Q000187:drug effects* / Q000648:ultrastructure; D010063:Ovum / Q000187:drug effects*; D011458:Prostaglandins E / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology*; D013997:Time Factors
D011458:Prostaglandins E; D011460:Prostaglandins F