Decrease and recovery in cell surface charge induced by anesthetics.
We applied cell electrophoresis to detect the effect of anesthetics on membrane, and clarified the concentration - effect relationship and the term of reversibility. The cell electrophoretic mobility of rat erythrocytes decreased with time of incubation with intravenous anesthetics (thiopental or droperidol) at clinical concentrations and reached a minimum value of 25% reduction in 30 min. The effect changed gradually in the concentration range of 0.5-1000 microgram/ml of thiopental, and changed markedly with 0.3-1 microgram/ml of droperidol. Washing out of the anesthetics and subsequent incubation in serum-containing medium resulted in the complete recovery in the mobility when 5 microgram/ml thiopental or 1 microgram/ml droperidol was used. With high concentration of thiopental (100 microgram/ml) or droperidol (10 microgram/ml, however, unrecovered fraction of cells increased with time of treatment. The critical time for retaining the reversibility was about 2 hr.
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D004586:Electrophoresis; D004912:Erythrocytes / Q000187:drug effects; D066298:In Vitro Techniques; D051381:Rats; D013499:Surface Properties; D013874:Thiopental / Q000494:pharmacology
D000777:Anesthetics; D013874:Thiopental