title: Evaluation of avian mycoplasma membranes as antigens.
abstract: Mycoplasma gallisepticum and M. synoviae membranes were obtained by lysing mycoplasma cells with carbonate bicarbonate buffer at pH 10. Membrane preparations were compared with whole-cell preparations as to efficacy in the plate and tube agglutination and hemagglutination-inhibition tests. Membrane antigens were somewhat more sensitive in the plate and tube agglutination tests, whereas the hemagglutination-inhibition test showed no major difference between whole and membrane antigens. The membrane preparations were equal to whole-cell antigens in specificity.
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000302:isolation & purification; D002462:Cell Membrane / Q000276:immunology; D002645:Chickens / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D009174:Mycoplasma / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial