Inactivation of cathepsin D by dithiophosgene and by 2,2-dichloro-1,3-dithiacyclobutanone.
Cathepsin D, purified from bovine spleen, is inactivated by the unstable inhibitors dithiophosgene and 2,2-dichloro-1,3-dithiacyclobutanone. Inhibition constants are identical for both of the compounds tested: Ki 96.1 muM;k/c0.406. It appears that the active species is 2,2-dichloro-1,3-dithiacyclobutanone, to which dithiophosgene is hydrolysed before cathepsin D inactivation.
D000818:Animals; D002074:Butanones / Q000494:pharmacology; D002403:Cathepsins / Q000037:antagonists & inhibitors*; D002417:Cattle; D006571:Heterocyclic Compounds / Q000494:pharmacology*; D010705:Phosgene / Q000031:analogs & derivatives / Q000494:pharmacology; D013154:Spleen / Q000201:enzymology; D013440:Sulfides / Q000494:pharmacology*; D013997:Time Factors
D002074:Butanones; D006571:Heterocyclic Compounds; D013440:Sulfides; D010705:Phosgene; C012949:dithiophosgene; C012893:2,2-dichloro-1,3-dithiacyclobutanone; D002403:Cathepsins