[A quantitative assessment of enzyme activity by cytophotometry using a standard].
The usage of the standard has been offered in cytospectrophotometrical measurements of enzymatic activities that are revealed histochemically. A tetrazolium method was used to demonstrate lactate dehydrogenase activity in sections of the rat brain. As a standard, a mixture of the enzyme (a crystalline forme of LDG) and gelatine was taken. The method of standardization permits fulfilling a quantitative comparison of heterogenic biological materials.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D004798:Enzymes / Q000378:metabolism*; D006651:Histocytochemistry / Q000379:methods*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051381:Rats
D004798:Enzymes; D007770:L-Lactate Dehydrogenase
Kolichestvennaia otsenka aktivnosti fermentov metodom tsitofotometrii s primeneniem étalona