Highly specific bacteriophage-associated polysaccharide hydrolases for Klebsiella aerogenes type 8.
Two phage-bound polysaccharide hydrolases specific for Klebsiella aerogenes type 8 exopolysaccharides were isolated. Each enzyme was specific for the polysaccharide produced by the host strain. One enzyme hydrolysed a pyruvylated and acetylated polymer, while the other only acted on the substrate lacking these substituents. Both enzymes were endogalactosidases releasing tetrasaccharides from their substrates which were only hydrolysed to a limited extent.
D000085:Acetates / Q000032:analysis; D001435:Bacteriophages / Q000201:enzymology*; D004754:Enterobacter / Q000032:analysis / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000378:metabolism*; D005561:Formates / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D005965:Glucuronates / Q000032:analysis; D006026:Glycoside Hydrolases / Q000378:metabolism*; D009844:Oligosaccharides / Q000096:biosynthesis; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000378:metabolism*; D011773:Pyruvates / Q000032:analysis; D013237:Stereoisomerism
D000085:Acetates; D005561:Formates; D005965:Glucuronates; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D011773:Pyruvates; D006026:Glycoside Hydrolases; D005947:Glucose; D005690:Galactose