title: Chemical and physical properties of the human urinary glycoprotein with gastric antisecretory activity.
abstract: The chemical and physical properties of the high-molecular-weight glycoprotein (SO20, w = 8S; Ve=Vo on Sephadex G-200) with gastric antisecretory activity extracted from the urine of pregnant women were studied. Gel filtration in the presence of sodium dodecyl sulphate and sodium dodecyl sulphate/polyacrylamide-disc-gel electrophoresis indicated subunit mol.wts. of 16 000 +/- 1500 and 13 000 +/- 1000 respectively. Reaggregation of the subunits and partial recovery of the biological activity were observed on removal of the detergent. The partial C-terminal sequence was found to be Phe-Tyr-Leu-Val-OH, whereas glycine appears to be the N-terminal amino acid. The carbohydrate composition was examined; all galactosamine was found to be O-glycosidically linked to the polypeptide chain.
mesh_terms: D000595:Amino Acid Sequence; D002241:Carbohydrates / Q000032:analysis; D005260:Female; D005688:Galactosamine; D005750:Gastric Juice / Q000187:drug effects / Q000378:metabolism*; D005998:Glycine; D006023:Glycoproteins / Q000494:pharmacology / Q000652:urine*; D006801:Humans; D008970:Molecular Weight; D011247:Pregnancy
chemical_list: D002241:Carbohydrates; D006023:Glycoproteins; D005688:Galactosamine; D005998:Glycine