The use of nylon fibre as a method of separating human lymphocyte subpopulations.
When the mononuclear cell population separated from normal human blood is passed through a nylon fibre column, monocytes and surface Ig bearing cells are retained; the eluted cells are a mixed properties. It appears that a proportion of the T cells eluted form EA-rosettes, and the eluted cell subpopulation includes non-T cells which share with B cells the ability to bind both EA and aggregated human IgG.
D002469:Cell Separation; D003176:Complement C3 / Q000032:analysis; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007104:Immune Adherence Reaction; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology; D009757:Nylons; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D003176:Complement C3; D007141:Immunoglobulin Fc Fragments; D009757:Nylons; D011947:Receptors, Antigen, B-Cell