Detection of antibodies of the IgM class in sera of patients recently infected with influenza viruses.
Sucrose density gradient ultracentrifugation can be used to detect specific 19S antibodies of the IgM class in the sera of patients recently infected with influenza A virus, provided steps are taken to remove non-specific inhibitors of haemagglutination. The usefulness of the procedure for the diagnosis of influenza requires further evaluation.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002499:Centrifugation, Density Gradient / Q000379:methods; D002645:Chickens / Q000276:immunology; D010856:Columbidae / Q000276:immunology; D005260:Female; D006384:Hemagglutination / Q000187:drug effects; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis*; D009980:Influenza A virus / Q000276:immunology*; D007251:Influenza, Human / Q000175:diagnosis / Q000276:immunology*; D008623:Mercaptoethanol / Q000494:pharmacology; D008970:Molecular Weight; D009975:Orthomyxoviridae / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008623:Mercaptoethanol