The use of frozen-stored sheep erythrocytes for demonstrating lymphocytic membrane markers.
The usefulness of cryopreserved sheep erythrocytes (unsensitized or coated with fluorescein, IgG-type antibody or complement) for marker tests on human blood lymphocytes was studied. It appears that the rosetting properties or freshly prepared or frozen-stored erythrocytes are not significantly different. The method may be used to provide homogeneous stocks of sensitized erythrocytes for lymphocyte rosetting technique.
D000818:Animals; D001793:Blood Preservation; D004912:Erythrocytes / Q000276:immunology; D005615:Freezing; D006801:Humans; D007104:Immune Adherence Reaction; D008214:Lymphocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell; D012756:Sheep
D011947:Receptors, Antigen, B-Cell