Radioimmunoassay of hepatitis B core antigen and antibody with autologous reagents.
Anti-HBc is the most sensitive indicator of current or recent hepatitis B virus infection, hence its detection is valuable in laboratory diagnosis of the infection. Dane particles isolated from the plasma of a single chronic carrier and his autologous IgG labeled with 125I were utilized in a solid-phase sandwich radioimmunoassay for anti-HBc. The principle of serologic neutralization of core antigen and consequent inhibition of binding of the cores to the solid-phase rendered the method to be a simple, sensitive, specific and rapid means of detecting anti-HBc.
D000914:Antibodies, Viral / Q000032:analysis*; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006512:Hepatitis B Core Antigens / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods
D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens