Biochemical changes in unilateral brain injury in the rat: A possible role of free fatty acid accumulation.
Cerebral energy metabolism was investigated in rats with the unilateral brain injury produced by the combination of left carotid artery ligation and systemic exposure to hypoxia. ATP and phosphocreatine levels in the left hemisphere were progressively reduced after the hypoxic-ischemic insult. The reduction of high-energy phosphate levels was accompained by an increase in sodium content and a decrease in potassium content. Mitochondria isolated from the damaged hemisphere showed a defect in ATP formation and oxygen uptake with a reduced ATP/O ratio. A large amount of free fatty acids (palmitic, stearic, oleic and arachidonic acids) accumulated in the injured hemisphere. The addition of unsaturated fatty acids (including oleic and arachidonic acids) to mitochondrial preparations caused an impairment of oxidative phosphorylation similar to that observed in mitochodria isolated from the damaged hemisphere.
D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001095:Arachidonic Acids / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D004573:Electrolytes / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D002534:Hypoxia, Brain / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009829:Oleic Acids / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010169:Palmitic Acids / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D013229:Stearic Acids / Q000378:metabolism; D014867:Water / Q000378:metabolism
D001095:Arachidonic Acids; D004573:Electrolytes; D005230:Fatty Acids, Nonesterified; D007773:Lactates; D009829:Oleic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; D010725:Phosphocreatine; D014867:Water; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D005947:Glucose