Method of obtaining drug-macromolecule binding parameters directly from dynamic dialysis data.
A new method of treating dynamic dialysis data to obtain binding parameters for drug-macromolecule interactions is presented. This method allows the determination of binding parameters directly from dialysis data according to a theoretical model. It is not necessary to determine the dialysis rate constant accurately in a separate experiment, and bias is not introduced due to differentiation. The proposed method should be applicable where the drug is substantially bound to the dialysis membrane.
D000418:Albumins / Q000378:metabolism; D002747:Chlorpropamide / Q000378:metabolism; D003956:Dialysis; D007700:Kinetics; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D008954:Models, Biological; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011485:Protein Binding
D000418:Albumins; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D004364:Pharmaceutical Preparations; D002747:Chlorpropamide