Improved continuous-flow method for detemination of total serum hexosamines.
We describe an automated determination of serum hexosamine by the Elson-Morgan reaction, together with reagent modifications that minimiz interference from amino acids and sugars present in acid hydrolysates of sera. We used a novel 15-min autoclave procedure for the acid hydrolysis of sera before analysis to facilitate the detemination as compared to the 4-h hydrolysis used in the conventional manual method. Results correlated well (r = 0.906) with those obtained by the corresponding manual method.
D001322:Autoanalysis; D003124:Colorimetry / Q000379:methods; D005069:Evaluation Studies as Topic; D005944:Glucosamine / Q000097:blood; D006595:Hexosamines / Q000097:blood*; D006801:Humans; D008239:Lysine
D006595:Hexosamines; D008239:Lysine; D005944:Glucosamine