Large-scale isolation of tRNA from barley embryos.
1. Large-scale isolation of tRNA from barley embryos is described, involving: phenol extraction, RNA deproteinization with the chloroform-isoamyl alcohol mixture, batch sorption on DEAE-cellulose, NaCl gradient elution of tRNA from DEAE-cellulose, and deaminoacylation of tRNA in the presence of bentonite. The procedure yielded tRNA free of protein and RNase activity. 2. The amino acid acceptor activity of the crude barley tRNA, its melting profiles and chromatographic patterns on Sephadex G-100 and BD-cellulose were similar to those of tRNA from other sources.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001467:Hordeum / Q000378:metabolism; D006358:Hot Temperature; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D012343:RNA, Transfer / Q000302:isolation & purification*; D012639:Seeds / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases