title: Extraction of gastrin from normal porcine pancreas.
abstract: Gastrin was extracted from normal porcine pancreas by the method used by Gregory and Tracy for the extraction of gastrin from the antrum. Radioimmuno-assay was used for identification at all stages of the extraction procedure. Gastrin of pancreatic origin was eluted from a sephadex G 50 and a DEAE-collulose column at the soma position as porcine antral gastrin. In disc electrophoresis (7.5% polyacrylamide in tris-glycin buffer) we found a single band which migrated the same distance from the origin as reference gastrin (synthetic human gastrin I). Densitometircally there was also a single band. The eluate of this electrophoretic band produced an increase in gastric acid secretion after intravenous injection in the rat. A dilution series was made from the extracted fraction and radioimmunologically tested with gastrin antibodies. The individual points of the dilution curve corresponded well with the standard calibration curve of gastrin. A certain discrepancy between the small amount of gastrin found by radioimmunoassay (500 pg/g fresh pancreas) and biochemical and biological findings could probably be explained by a different sensitivity of the gastrin antibody of the radioimmunological system to pancreatic gastrin. Our results prove that there is gastrin in the normal porcine pancreas.
mesh_terms: D000818:Animals; D005750:Gastric Juice / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects; D005755:Gastrins / Q000008:administration & dosage / Q000032:analysis*; D008722:Methods; D010179:Pancreas / Q000032:analysis*; D051381:Rats; D013552:Swine
chemical_list: D005755:Gastrins