Chlorpromazine metabolism VII: new quantitative fluorometric determination of chloropromazine and its sulfoxide.
A new, sensitive assay is described for chlorpromazine and/or its sulfoxide. The method is based on reacting the tertiary amine base with 9-bromomethylacridine to form a quaternary compound which, on photolysis, yields highly fluorescent products that are determinable fluorometrically. The procedural steps were standardized, and an optimum assay procedure was developed. The method shows a less than 3% coefficient of variation when applied directly to chlorpromazine samples and is capable of determining 15-20 ng of the drug. The method is readily adaptable to clinical and bioavailability studies.
D055598:Chemical Phenomena; D002621:Chemistry; D002746:Chlorpromazine / Q000031:analogs & derivatives* / Q000032:analysis*; D002855:Chromatography, Thin Layer; D005470:Fluorometry; D008722:Methods; D010782:Photolysis; D013454:Sulfoxides / Q000032:analysis; D013696:Temperature; D013997:Time Factors
D013454:Sulfoxides; D002746:Chlorpromazine