Studies on human N-acetyl-Beta-d-hexosaminidase C separated from neonatal brain.
Human brain hexosaminidase C was separated from isoenzymes A and B by Sephadex G-200 gel filtration. Properties of the enzyme were studied, particularly its isoelectric-focusing profile, pI4.80. These findings indicate that hexosaminidase C is identical with the major residual component of Sandhoff fibroblasts with respect to substrate specificity, pI and activity pH optimum.
D001921:Brain / Q000201:enzymology*; D002850:Chromatography, Gel; D005260:Female; D005313:Fetal Death; D006596:Hexosaminidases / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D006923:Hymecromone / Q000378:metabolism; D007231:Infant, Newborn; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis* / Q000378:metabolism; D007700:Kinetics; D011247:Pregnancy
D007527:Isoenzymes; D006923:Hymecromone; D006596:Hexosaminidases