Limits of the estimate of serum folate-binding protein by radioassay.
Various factors that influence the measurement of serum folic acid-binding protein (FABP) by radioassay were examined. Varying the protein concentration and the composition of the charcoal suspension can increase or reduce the value of the blank, i.e. the estimate of FABP. Moreover, it was found that the value of the FABP depends on the quantity of folic acid in the incubation medium. Reproducible results can only be obtained with rigorously defined conditions of concentration of the different reactants.
D001798:Blood Proteins / Q000378:metabolism*; D002606:Charcoal; D005069:Evaluation Studies as Topic; D005492:Folic Acid / Q000097:blood*; D006801:Humans; D008832:Microchemistry; D011485:Protein Binding; D011869:Radioligand Assay / Q000379:methods
D001798:Blood Proteins; D002606:Charcoal; D005492:Folic Acid