Light-induced formation of the 410 nm intermediate from reconstituted bacteriorhodopsin.
Light-induced formation of the 410 nm intermediate was investigated with reconstituted bacteriorhodopsin. Only bacteriorhodopsin with all-trans retinal formed the 410 nm intermediate in response to light excitation. In equilibrium in the dark, both native and reconstituted bacteriorhodopsin contained about equal amounts of the all-trans and 13-cis forms over a wide range of temperature.
D001436:Bacteriorhodopsins / Q000528:radiation effects*; D002338:Carotenoids / Q000528:radiation effects*; D006217:Halobacterium; D007700:Kinetics; D008027:Light; D012172:Retinaldehyde
D002338:Carotenoids; D001436:Bacteriorhodopsins; D012172:Retinaldehyde