[The effect of the concentration and nature of 1-1-valent electrolytes on acid-base equilibrium in solutions of DNA].
The effect of concentration of LiCl, NaCl, KCl and NH4Cl on the potentiometric titration curves of chicken erythrocyte DNA has been studied. Two inflections have been found in the titration curves at pH 5 and pH 8, respectively. The ionization constant corresponding to the second inflection is slightly increased when the concentrations of NaCl, KCl and NH4Cl are increased. Both constants are increased when the concentration of LiCl is increased. It has also been found that the addition of LiCl to the DNA solution lowers the pH of the solution but the addition of the other investigated salts causes an increase of the pH of DNA solutions. The results found are used for discussion of the nature of acid groups of DNA.
D000136:Acid-Base Equilibrium; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D004247:DNA; D004573:Electrolytes; D004912:Erythrocytes / Q000032:analysis; D007700:Kinetics; D012965:Sodium Chloride; D012996:Solutions
D004573:Electrolytes; D012996:Solutions; D012965:Sodium Chloride; D004247:DNA
Vliianie kontsentratsii i prirody 1-1-valentnykh élektrolitov na kislotno-osnovnoe ravnovesie v rastvorakh DNK