Effects of delta9-tetrahydrocannabinol in Lewis lung adenocarcinoma cells in tissue culture.
We found a dose-related decrease in DNA synthesis in transformed cell cultures treated with delta9-tetrahydrocannabinol (delta9-THC). The decrease, observed over a 4-hour period, was not accompanied by a change in the radioactive precursor pool as compared to that of control culture. The distribution of labeled products clearly differed from that observed after treatment with cytosine arabinoside. delta9-THC inhibited DNA synthesis at some point beyond the uptake of 3H-thymidine.
D000230:Adenocarcinoma / Q000378:metabolism*; D000818:Animals; D002188:Cannabis / Q000494:pharmacology*; D046508:Culture Techniques; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D013759:Dronabinol / Q000494:pharmacology*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D004273:DNA, Neoplasm; D013759:Dronabinol; D013936:Thymidine