title: Further investigation on the radiation induced inactivation of ribonuclease and the radioprotective effect of some selenium-containing compounds.
abstract: Steady state inactivation data on dilute aqueous solutions of RNase show that all water radicals, e-aq, OH, and H are responsible for the inactivation, but the most efficient radical is H atom, only about 4 of them being required for one inactivating event. The data are, therefore, more in agreement with the conclusions of Mee et al. (1972). In the transient absorption spectra of pulse irradiated ribonuclease different components derived by the individual radicals are observed. Organic and inorganic selenium-containing compounds offer a great protection of the enzyme activity, in agreement with the data obtained in other chemical and biological systems. In particular the effects of two new secondary radicals (CNSe)-2 and SeO-3 are in good accord with the known structure of ribonuclease.
mesh_terms: D007700:Kinetics; D012260:Ribonucleases / Q000037:antagonists & inhibitors / Q000528:radiation effects*; D012643:Selenium / Q000494:pharmacology*; D012645:Selenomethionine / Q000494:pharmacology; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012645:Selenomethionine; D012260:Ribonucleases; D012643:Selenium