Isolation of canine prolactin by polyacrylamide gel electrophoresis.
The electrophoretic mobility of prolactin obtained from canine pituitary extract was studied with the aid of polyacrylamide disc electrophoresis. Using a preparative gel electrophoretic system the immunoreactive material was purified on a quantitative scale which was then used to develop a homologous radioimmunoassay for canine prolactin. The radioimmunoassay system was able to detect prolactin in the plasma of dogs after the administration of agents which would be expected to affect prolactin secretion.
D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D002746:Chlorpromazine / Q000494:pharmacology; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004971:Estrus; D005260:Female; D008297:Male; D010546:Perphenazine / Q000494:pharmacology; D010902:Pituitary Gland / Q000032:analysis; D011247:Pregnancy; D011388:Prolactin / Q000097:blood / Q000302:isolation & purification*; D011863:Radioimmunoassay
D001971:Bromocriptine; D011388:Prolactin; D010546:Perphenazine; D002746:Chlorpromazine