[Electrophoretic characteristics of the proteins and the preparative production of neuraminidase from the A/turkey/Wisconsin/66 (Hav6N2) strain of the avian influenza virus].
A combination of electrophoresis in a single plate of polyacrylamide gel with preparative production of subunits upon electrophoresis in acetate cellulose was used for the analysis of the polypeptide composition of the A/turkey/Wisconsin/66 strain. Seven classes of proteins with certain functional significance and 4 minor components were detected. A preparation of neuraminidase could be obtained which has enzymatic and antigenic activity, and a molecular mass of 68,000 daltons. Some features of the polypeptide composition were revealed, including the presence of uncleaved hemagglutinin under reducing conditions and a decreased content of the light chain of hemagglutinin.
D004588:Electrophoresis, Cellulose Acetate; D004591:Electrophoresis, Polyacrylamide Gel; D006387:Hemagglutination, Viral; D009980:Influenza A virus / Q000032:analysis* / Q000201:enzymology; D008970:Molecular Weight; D009439:Neuraminidase / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis*
D010455:Peptides; D014764:Viral Proteins; D009439:Neuraminidase
Elektroforeticheskaia kharakteristika belkov i preparativnoe poluchenie neĭraminidazy shtamma A/indiuk/Viskonsin/66 (Hav6N2) virusa grippa ptits.