title: High-performance liquid chromatographic analysis of methadone hydrochloride oral solution.
abstract: A direct and rapid high-performance liquid chromatographic assay for methadone hydrochloride in a flavored oral solution dosage form is described. A syrup sample, one part diluted with three parts of water, is introduced onto a column packed with octadecylsilane bonded on 10 micrometer porous silica gel (reversed phase). A formic acid-ammonium formate-buffered mobile phase is linear programmed with acetonitrile. The absorbance is monitored continuously at 280 or 254 nm, using a flow-through, UV, double-beam photometer. An aqueous methadone hydrochloride solution is used for external standardization. The relative standard deviation was not more than 1.0%. Drug recovery from a syrup base was better than 99.8%.
mesh_terms: D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid; D008691:Methadone / Q000032:analysis*; D012996:Solutions / Q000032:analysis
chemical_list: D012996:Solutions; D008691:Methadone