[Scanning intergrating cytospectophotometry of tightly apposed micro-objects (as an example, a study of the circadian rhythm of RNA and protein concentrations in different neuron--neuroglia systems)].
A scheme of electron aperture is described which permits a scanning intergrating cytospectrophotometry of adjacent microobjects. Results are presented of such cytophotometry to determine RNA and protein content in various neuron--neuroglia units of the rats studied every 4 hours during 24 hours period. Contraphase character of circadian fluctuations has been shown for RNA level in cerebllum Purkinje cells, hypothalamus supraoptic neurons and spinal cord motoneurons as compared with the fluctuations in corresponding glial satellite cells. Protein content fluctuations were of contraphase character only in the spinal motoneurons and in their perineuronal glia, whereas in all the other neuron-neuroglia units the period of fluctuations was rather similar in the neurons and in the glial cells. A great value of quantitative cytochemical methods is outlined to carry out a functional biochemical analysis of nervous tissue cells.
D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D002940:Circadian Rhythm; D004247:DNA / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods*; D008297:Male; D009046:Motor Neurons / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D011689:Purkinje Cells / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D013053:Spectrophotometry / Q000379:methods*; D013116:Spinal Cord / Q000166:cytology; D013495:Supraoptic Nucleus / Q000166:cytology
D009419:Nerve Tissue Proteins; D012313:RNA; D004247:DNA
Skaniruiushcaia intergriruiushchaia tsitospektrofotometriia tesono soprikasaiushchikhsia mikroob ektov (na primere izucheniia sutochnogo ritma soderzhaniia RNK ibelka v razlichnykh sistemakh neĭron--neĭrogliia)