Serological techniques for detection of antibody to galactocerebroside.
Two serological techniques were developed for the detection of antibody to galactocerebroside using liposomes as a carrier for the lipid hapten. One assay is a radioimmunoprecipitation test employing [3H] cholesterol as a marker in the galactocerebroside-liposomes. The other is a less sensitive but quick and easy galactocerebroside-liposome agglutination assay. Specificity is demonstrated by comparison of titers when other lipid haptens replace galactocerebroside in the liposomes, and when other anti-glycolipid antisera are reacted with galactocerebroside-liposomes.
D000372:Agglutination Tests; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D002417:Cattle; D002518:Ceramides / Q000276:immunology; D002554:Cerebrosides / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology; D008081:Liposomes / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D013109:Sphingomyelins / Q000276:immunology
D000906:Antibodies; D002518:Ceramides; D002554:Cerebrosides; D005732:Gangliosides; D008081:Liposomes; D013109:Sphingomyelins