title: Salivary epithelial cells in primary culture: characterization of their growth and functional properties.
abstract: Mouse submandibular salivary gland cells were grown in primary explant culture. After an initial period of degeneration within the explant, surviving epithelial cells proliferated rapidly and duct-like structures recolonized the explant. Autoradiographic studies showed that a peak of DNA synthesis occurred after 4 days in vitro and that proliferation was enhanced by insulin and hydrocortisone. These cells retained specialized secretory function (protease activity) for at least 2 weeks in vitro. This enzyme is a differentiated product of granular tubule cells in vivo. Between 6 and 10 days, explants attached to the substrate. An outgrowth developed, consisting largely of ultrastructurally identifiable epithelial cells which formed pseudoglandular structures in the monolayer. Epithelium survived for over 6 months in primary culture but could not be serially transferred. Secondary cultures were rapidly overgrown by mesenchymal cells.
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D002465:Cell Movement; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism; D003470:Culture Media; D003896:Desmosomes / Q000648:ultrastructure; D004847:Epithelial Cells; D006651:Histocytochemistry; D006854:Hydrocortisone; D006913:Hydroxysteroid Dehydrogenases / Q000032:analysis; D007328:Insulin; D007365:Intercellular Junctions / Q000648:ultrastructure; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000209:etiology; D010447:Peptide Hydrolases / Q000032:analysis; D013363:Submandibular Gland / Q000166:cytology*; D013739:Testosterone / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013974:Thyroxine / Q000494:pharmacology
chemical_list: D003470:Culture Media; D007328:Insulin; D013739:Testosterone; D006913:Hydroxysteroid Dehydrogenases; D010447:Peptide Hydrolases; D007545:Isoproterenol; D013974:Thyroxine; D013936:Thymidine; D006854:Hydrocortisone