EM autoradiography of non-sectioned biological material.
Methods of EM autoradiography suitable for the study of non-sectioned biological material deposited on a support film from suspension are described. The methods, which include modified procedures for specimen support and emulsion preparation, may also have advantages for the study of any specimen where physical separation of specimen and emulsion is desirable. In a study of the growth of 125I-labelled collagen fibrils reconstituted from solution in vitro, these procedures have allowed the production of EM autoradiographs which show a high resolution and are free from stress artefacts and chemography.
D001345:Autoradiography / Q000379:methods*; D003094:Collagen / Q000502:physiology*; D008854:Microscopy, Electron
D003094:Collagen