title: The effect of colchicine and vinblastine on the release of pulmonary surface active material.
abstract: The secretion of phosphatidylcholine was studied by incubating hamster lung slices which had been prelabeled by the in vivo administration of 14C-labeled choline. The release of 14C-labeled phospholipid into the medium continued for 2 hr. The specific activity of phosphatidylcholine in the medium was one-third that found in the tissue, and the fatty acid composition of the released phosphatidylcholine corresponded to that of surfactant. The prior injection of colchicine resulted in a 60% inhibition of phosphatidylcholine release into the incubation medium. A similar effect was obtained when vinblastine was administered. Colchicine demonstrated no inhibiting effect on the release of protein from lung slices which had been prelabeled by the administration of 14C-labeled leucine. The possible function of the microtubular system in the secretion of surface active components is discussed.
mesh_terms: D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D006224:Cricetinae; D066298:In Vitro Techniques; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D014176:Protein Biosynthesis / Q000187:drug effects; D011663:Pulmonary Surfactants / Q000378:metabolism*; D014747:Vinblastine / Q000494:pharmacology*
chemical_list: D011663:Pulmonary Surfactants; D014747:Vinblastine; D007930:Leucine; D003078:Colchicine