Influenza virus RNA's in the cytoplasm of chicken embryo cells treated with 3'-deoxyadenosine.
3'-Deoxyadenosine (75 to 100 mug/ml) permitted analysis of the cytoplasmic influencza virus-specific RNAs synthesized early in the replicative cycle-a phase that has hitherto been obscurred by host cell RNA synthesis. In addition, late in the cycle (6 to 8) complementary virus-specific RNA's were the predominantly labeled species, suggesting that higher concentrations of 3'-deoxyadenosine selectively inhibit influenza viral genome replication.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D046508:Culture Techniques; D003593:Cytoplasm / Q000032:analysis; D003609:Dactinomycin / Q000494:pharmacology; D009975:Orthomyxoviridae / Q000032:analysis* / Q000254:growth & development; D012313:RNA / Q000096:biosynthesis*; D012367:RNA, Viral / Q000032:analysis*; D014158:Transcription, Genetic; D014779:Virus Replication
D012367:RNA, Viral; D003609:Dactinomycin; D012313:RNA