title: The distribution of halofenate in plasma: a comparative analysis using Scatchard vs. stepwise association constants.
abstract: The distribution of Halofenate between the free and albumin bound forms was calculated by the use of two computer programs using both Scatchard association constants for a 3,3,7 model, and six stepwise equilbrium constants over a range of drug concentrations reported in man. The calculations, using either set of constants, showed that only 0.3 to 0.5% of the drug would be free. Using Scatchard association constants, it was estimated that 92 to 95% of the drug would be bound by the high affinity set of sites, and lesser amounts by sites of lesser affinity. A more complex pattern of distribution was obtained with the stepwise equilibrium constants. At low concentrations of Halofenate the complex with one mole of drug/mole of protein was most abundant and at the highest concentration studied the complex with two moles of drug/mole of protein was most abundant.
mesh_terms: D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism; D003201:Computers; D006016:Glycolates / Q000097:blood*; D006218:Halofenate / Q000097:blood*; D006801:Humans; D008722:Methods; D011485:Protein Binding
chemical_list: D001798:Blood Proteins; D006016:Glycolates; D006218:Halofenate