Purification of alkaline phosphatase of the halotolerant yeast Debaryomyces hansenii.
Alkaline phosphatase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.1) of the halotolerant yeast Debaryomyces hansenii was purified by a procedure involving cell disruption, DNAase treatment, ethanol precipitation, gel filtration, chromatography on DEAE-Sephadex, and preparative polyacrylamide gel electrophoresis. The specific activity was increased 1250-fold as compared to the activity of cell free extract. The total recovery was 30%. Various modifications of the growth conditions had slight or no effect on the yield of enzyme.
D000469:Alkaline Phosphatase / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003851:Deoxyribonucleases; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol; D015003:Yeasts / Q000201:enzymology*
D000431:Ethanol; D003851:Deoxyribonucleases; D000469:Alkaline Phosphatase