Adenovirus protein maturation at 42 degrees C.
Incubation of adenovirus type 2 infected cells at 42 degrees C resulted in an inhibition of assembly of virus particles although all the major viral structural polypeptides and virus-induced cellular polypeptides so far identified were detected by electrophoretic analysis. Selective high salt-acid-urea extraction of low mol. wt. polypeptides revealed the absence of protein VII at 42 degrees C whereas precursor polypeptide P-VII and core protein V were found. Pulse-chase and temperature shift experiments indicated that cleavage of P-VII into VII was a reversible thermosensitive process, requiring de novo protein synthesis after shift-down to 37 degrees C. Virus particles assembled at 37 degrees C after transfer from 42 to 37 degrees C contained both viral DNA and polypeptides pre-labelled during the eclipse phase at 42 degrees C, including core protein VII.
D000256:Adenoviridae / Q000032:analysis / Q000378:metabolism*; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004279:DNA, Viral / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis / Q000378:metabolism*
D004279:DNA, Viral; D010455:Peptides; D011498:Protein Precursors; D014764:Viral Proteins; D003609:Dactinomycin; D003513:Cycloheximide