title: Phycocyanin synthesis and degradation in the blue-green bacterium Anacystis nidulans.
abstract: Cellular content and rates of synthesis of the apoprotein subunits of phycocyanin in Anacystis nidulans cultures undergoing, and recovering from, nitrate starvation were measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total and immunoprecipitable soluble proteins. Results indicated that (i) nitrate starvation provokes coordinate degradation of apoprotein subunits: (ii) de novo synthesis of these subunits is selectively depressed during starvation; (iii) nitrate restoration provokes coordinate increases in the rates of synthesis of these subunits, although maximal rates are not achieved for 6 to 10 h after readdition of nitrate; and (iv) illumination affects both relative and absolute rates of apoprotein formation.
mesh_terms: D001059:Apoproteins / Q000096:biosynthesis / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D009566:Nitrates / Q000378:metabolism; D010798:Phycocyanin / Q000096:biosynthesis / Q000378:metabolism*; D010860:Pigments, Biological / Q000378:metabolism*
chemical_list: D001059:Apoproteins; D001426:Bacterial Proteins; D009566:Nitrates; D010860:Pigments, Biological; D010798:Phycocyanin