An electron microscopic study of the distribution of sodium in normal, pentagastrin-stimulated and secretin-inhibited fundic mucosa of the stomach of the rat.
Normal, pentagastrin-stimulated and secretin-inhibited stomachs of rats were fixed either by perfusion or by immersion in an unbuffered osmium tetroxide potassium pyroantimonate mixture at a pH of 7.6, and the mucosa from the fundic area was examined under the electron microscope for sodium localization. Both fixation methods gave comparable results. In the control tissue, sodium pyroantimonate crystals were mainly seen in and around the collagen fibers in the interstitium and to a lesser extent in the basal and lateral plasma membranes of the epithelial cells. No significant differences were noticed in the secretin-inhibited tissue. In contrast, in the pentagastrin-stimulated mucosa, an increase in the amount of precipitate along with a redistribution occurred. The pyroantimonate technique seems promising for studying the mechanism of sodium transport in the gastric mucosa.
D000818:Animals; D000965:Antimony; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D006651:Histocytochemistry; D008297:Male; D010418:Pentagastrin / Q000494:pharmacology*; D051381:Rats; D012633:Secretin / Q000494:pharmacology*; D012964:Sodium / Q000032:analysis / Q000378:metabolism*
D012633:Secretin; D000965:Antimony; D012964:Sodium; D010418:Pentagastrin