Effect of ischaemic limb injury on the rates of metabolism of ketone bodies in starved rats.
1. Rats starved for 30h were injected with trace amounts of [3-14C]acetoacetate and beta-hydroxy[3-14C]butyrate 1h after ischaemic limb injury in a 20 degrees C environment, and the concentrations and radioactivities of blood ketone bodies were determined at intervals. 2. Starvation alone raised the rates of production and utilization of beta-hydroxybutyrate plus acetoacetate about 3.7-fold, but lowered their metabolic clearance rates by about 50%. In the starved rat ketone-body oxidation could account for up to 30% of whole body O2 consumption. 3. Injury in starved rats lowered the rates of production and utilization of both beta-hydroxybutyrate and acetoacetate, the combined fall of about 37% slightly exceeding the concomitant fall in whole-body O2 consumption. The concentration of beta-hydroxybutyrate decreased after injury, but its metabolic clearance rate was unaltered; the concentration of acetoacetate rose slightly and its metabolic clearance rate fell.
D000090:Acetoacetates / Q000097:blood* / Q000378:metabolism; D000818:Animals; D001810:Blood Volume; D006498:Hepatectomy; D006614:Hindlimb / Q000098:blood supply*; D006885:Hydroxybutyrates / Q000097:blood* / Q000378:metabolism; D007511:Ischemia / Q000097:blood*; D008297:Male; D008657:Metabolic Clearance Rate; D051381:Rats; D013217:Starvation / Q000097:blood*; D013997:Time Factors
D000090:Acetoacetates; D006885:Hydroxybutyrates