Disappearance rates of intravenously injected canine alkaline phosphatase isoenzymes.
The serum half-life of various canine alkaline phosphatase isoenzymes has been determined. Isoenzymes can be divided into 2 groups with regard to their half-life. Isoenzymes with a half-life of approximately 3 days include the hepatic alkaline phosphatase and the steroid-induced alkaline phosphatase. Those with a half-life of under 6 minutes include the alkaline phosphatase from placenta, kidney, intestine, and the hepatic and steroid-induced iosenzymes after removal of sialic acid. The inability to demonstrate some of the isoenzymes of alkaline phosphatase in serum of diseases animals is attributed to their short half-life. The relationship of the short half-life to the sialic acid moiety of the protein is discussed.
D000469:Alkaline Phosphatase / Q000008:administration & dosage / Q000032:analysis / Q000097:blood*; D000818:Animals; D004285:Dogs / Q000097:blood*; D004386:Duodenum / Q000201:enzymology; D005260:Female; D007275:Injections, Intravenous; D007527:Isoenzymes / Q000008:administration & dosage / Q000032:analysis / Q000097:blood*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D012794:Sialic Acids / Q000302:isolation & purification
D007527:Isoenzymes; D012794:Sialic Acids; D000469:Alkaline Phosphatase