A novel semisynthetic route to insulin analogues modified at the N-terminus of the A-chain.
We report a novel route for the semi-synthetic production of insulin analogues modified at the amino-terminus of the A-chain. The route proceeds from insulin via [Nalpha-Boc-GlyA1]-insulin to [Nalpha-(Z-methionyl)-PheB1, Nepsilon-(Z-methionyl)-LysB29]des-GlyA1-insulin. We have replaced residue A1 with glycine, to give a fully crystalline resynthesized insulin, and with D-lysine.
D000596:Amino Acids / Q000032:analysis; D007328:Insulin / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D008722:Methods; D013329:Structure-Activity Relationship
D000596:Amino Acids; D007328:Insulin