title: Formation of 3-methylthioacrylic acid from methionine by Streptomyces lincolnensis. Isolation of a peroxidase.
abstract: Cultures of Streptomyces lincolnensis accumulated 3-methylthioacrylic acid in amounts directly related to the concentration of methionine in the medium. The metabolite was labeled by L-(methyl-14C) but not by DL-(carboxyl-14C) methionine, indicating biosynthesis from the amino acid with loss of the carboxyl group. S. lincolnensis mycelium contained sufficient peroxidase activity to catalyse oxidative decarboxylation of L-methionine to 3-methylthiopropionamide as the initial step of a biosynthetic sequence. The enzyme, partially purified by ammonium sulfate precipitation, chromatography on a DEAE-cellulose column ang gel filtration, had a molecular weight of approximately 350,000, a pH optimum of 6.0, with o-dianisidine as electron donor and a Km value of 7.5 x 10(-4) M with respect to hydrogen peroxide. Cultures of S. lincolnensis supplemented with 3-(methyl-14C) methylthiopropionic acid gave labeled 3-methylthioacrylic acid. However, 3-(methyl-14C) methylthiopropionamide did not label the metabolite, suggesting that the first intermediate in the pathway may be the keto acid, which is then oxidatively decarboxylated to 3-methylthiopropionic acid.
mesh_terms: D000179:Acrylates / Q000302:isolation & purification / Q000378:metabolism*; D005656:Fungal Proteins / Q000032:analysis; D008689:Methacrylates / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D010544:Peroxidases / Q000302:isolation & purification; D013302:Streptomyces / Q000201:enzymology / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000179:Acrylates; D005656:Fungal Proteins; D008689:Methacrylates; D008715:Methionine; D010544:Peroxidases