title: Ultrastructure and chemical analysis of the cell wall of Pythium debaryanum.
abstract: The ultrastructure and component polysaccharides of the cell wall of Pythium debaryanum IFO-5919 were investigated. From results obtained by means of acid, alkali, Schweitzer reagent and beta-1, 3-glucanase treatments and electron microscopy, it was concluded that 1) the acid-extracted fraction was a 1,3-linked branched glucan, 2) the alkali-extranded fraction was a mixture of 1,3-, 1,6-, and 1,3,6-linked highly branched two glucans, 3) the Schweitzer reagent-extracted fraction was a beta-1, 4-linked glucan, 4) the cell wall was constructed from two types of cullulosic microfibrils, as a frame and as a finer network, and amorphous beta-1, 3-glucan including beta-1, 6-linkage, 5) cellulosic microfibrils were covered by matrix material consisting of a mixture of amorphous beta-1, 3-linked and beta-1, 6-linked branching glucans.
mesh_terms: D002473:Cell Wall / Q000032:analysis / Q000648:ultrastructure; D002482:Cellulose / Q000032:analysis; D005658:Fungi / Q000648:ultrastructure*; D008961:Models, Structural; D011134:Polysaccharides / Q000032:analysis; D011775:Pythium / Q000648:ultrastructure*
chemical_list: D011134:Polysaccharides; D002482:Cellulose