Comparison of control materials containing animal and human enzymes. Comparison of enzymes of human and animal origin, III.
Highly purified enzymes of diagnostic interest from human and animal organs, dissolved in pooled human serum and in bovine serum albumin solution, were compared with respect to their response to alterations in routine clinical chemical assay conditions. Their response to changes in temperature, substrate concentration and pH-value was the same. In addition, the storage stability in each matrix was identical in the lyophilized and the reconstituted state, whereas some enzymes were remarkably less stable in the pooled human serum than in bovine serum albumin. This better stability, the better availability and decreased infectious nature of the material lead to the conclusion that animal enzymes in bovine serum albumin matrix are the material of choice for the quality control of enzyme activity determinations in clinical chemistry.
D000818:Animals; D004355:Drug Stability; D004798:Enzymes / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D012710:Serum Albumin, Bovine; D013045:Species Specificity; D013552:Swine; D013696:Temperature; D000637:Transaminases / Q000378:metabolism
D004798:Enzymes; D007202:Indicators and Reagents; D012710:Serum Albumin, Bovine; D007770:L-Lactate Dehydrogenase; D000637:Transaminases