[Study of the stability of the alpha-ketoglutarate decarboxylase from the brain of a bull].
Stability of alpha-ketoglutarate decarboxylase (alpha-KGDC) from the bull brain was studied in different temperature regimes. The high-purified preparations of alpha-KGDC are highly unstable both when kept at a temperature of 0 degrees C and as affected by temperature. The least loss of the alpha-KGDC activity is observed in the preparations obtained after lyophilization and stored for a long time as powder. The temperature inactivation may be prevented by adding to the medium Ca2+ (10(-3) M) and TPP (10(-2) M) as well as TPP and Ca2+ and Mg2+ in different combinations. At low temperatures none of the mentioned components and such substances as histidine and serum albumin has no stabilizing effect on alpha-Kgdc preparations show that the inhibitory effect of the latter is more pronounced. It is peculiar to both less purified and highly purified alpha-KGDC preparations. The action of urea is less pronounced and is greatly similar by the inhibitory effect as compared to alpha-KGDC from the pigeon muscles.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002262:Carboxy-Lyases / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D010856:Columbidae; D004355:Drug Stability; D004356:Drug Storage; D004789:Enzyme Activation; D004794:Enzyme Repression / Q000187:drug effects; D007656:Ketoglutaric Acids; D007700:Kinetics; D008297:Male; D009132:Muscles / Q000201:enzymology; D013696:Temperature
D007656:Ketoglutaric Acids; D002262:Carboxy-Lyases; C021474:2-oxoglutarate decarboxylase
Izuchenie stabil'nosti al'fa-ketoglutaratdekarboksilazy iz mozga byka