A binding assay for 25-hydroxycalciferols and 24R,25-dihydroxycalciferols using bovine plasma globulin.
The concentrations of the 25-hydroxy and 24R, 25-dihydroxy derivatives of vitamin D were determined in 100 microliter l plasma samples using calciferol binding globulin from bovine plasma. Sufficient quantities of 24R, 25-dihydroxy vitamin D were found in bovine, porcine, chicken and human plasma to interfere in the assay of 25-hydroxy vitamin D in unfractionated extracts. No metabolites of vitamin D could be found in rainbow trout plasma.
D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D002417:Cattle; D002645:Chickens; D004100:Dihydroxycholecalciferols / Q000097:blood*; D006801:Humans; D006887:Hydroxycholecalciferols / Q000097:blood*; D008832:Microchemistry; D011869:Radioligand Assay / Q000379:methods; D012712:Serum Globulins; D013045:Species Specificity; D013552:Swine; D014337:Trout
D002352:Carrier Proteins; D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D012712:Serum Globulins