title: Construction of chimeric phages and plasmids containing the origin of replication of bacteriophage lambda.
abstract: Segments of the replication control region of bacteriophage lambda (lambda) and lambda mutants defective in replication were attached in vitro to the phi80 phage vector Charon 3 and to the plasmid vector mini Col El (pVH51). The chimeric phages and plasmids have been used to localize the origin of lambda DNA replication and to facilitate a structural analysis of the lambda replicator.
mesh_terms: D002874:Chromosome Mapping; D003090:Coliphages / Q000235:genetics*; D004261:DNA Replication; D004274:DNA, Recombinant / Q000235:genetics*; D005796:Genes; D005809:Genes, Regulator; D005814:Genes, Viral; D009154:Mutation; D010957:Plasmids; D014779:Virus Replication
chemical_list: D004274:DNA, Recombinant