Identification of beta-aminoisobutyric acid in uremic serum.
An unidentified ninhydrin-positive substance found in uremic sera but not found in normal sera was isolated by gel-filtration through Sephadex G-75 followed by high voltage paper electrophoresis (pH 3.5), and identified as beta-aminoisobutyric acid using paper chromatography and automated amino acid analyzer. The quantitative determination of beta-aminoisobutyric acid in serum revealed that the level of beta-aminoisobutyric acid in uremic sera was much higher than that of normal sera. Gas chromatographic determination of the enantiomorphs of beta-aminoisobutyric acid showed that uremic sera contain R- and S-isomers of the amino acid, but with the R-isomer as the dominating form.
D000621:Aminoisobutyric Acids / Q000097:blood*; D001806:Blood Urea Nitrogen; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D003404:Creatinine / Q000097:blood; D004590:Electrophoresis, Paper; D006801:Humans; D007676:Kidney Failure, Chronic / Q000652:urine; D006435:Renal Dialysis; D014511:Uremia / Q000097:blood*; D014527:Uric Acid / Q000097:blood
D000621:Aminoisobutyric Acids; D014527:Uric Acid; D003404:Creatinine