title: Calcium channels in Paramecium aurelia.
abstract: Reversal of swimming direction in paramecium is dependent on the calcium influx through the excitable-membrane calcium channels. Several mutants of Paramecium aurelia have been selected on the basis of their resistance to the paralyzing effect of barium. The mutants have reduced reversal behavior and are in the same three pawn genes as discovered by Kung (16, 17). Also, in barium solutions, the pawns live longer than the wild-type; however, pwB mutants are more resistant to barium toxicity than pwA mutants. These results suggest that the selection picked up mutants in the calcium channel. Electrophysiological studies demonstrate this point directly, showing defective calcium activation in all pawns, but also defective anomalous rectification in pwB mutants. A model is presented which accounts for the differences between pwA and pwB mutants. It ascribes the depolarization-sensitive "gate" function to the pwA gene product and the "pore" function to the pwB gene product. Additionally, the stability of the channel structure is demonstrated, channel half-life being from five to eight days.
mesh_terms: D000818:Animals; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D008954:Models, Biological; D009043:Motor Activity / Q000187:drug effects; D010247:Paramecium / Q000235:genetics / Q000378:metabolism*
chemical_list: D001464:Barium; D002118:Calcium