Inhibition of thymidine uptake by staphylococci, a new method for the investigation of phagocytosis.
A radioassay employing (3H) thymidine, to measure inhibition of growth of Staphylococcus aures by human phagocytes is presented. The principle of this new method is that viable and dividing staphylococci take up thymidine more rapidly than white cells, so that whereas in control cultures containing staphylococci alone high counts per minute (cpm) are obtained within 90 min of incubation in test cultures both leucocytes and plasma from 25 normal subjects reduced the cpm, following ingestion and killing of staphylococci.
D001772:Blood Cell Count; D006801:Humans; D010586:Phagocytes; D010587:Phagocytosis; D011863:Radioimmunoassay / Q000379:methods; D013211:Staphylococcus aureus / Q000254:growth & development*; D013936:Thymidine / Q000378:metabolism*; D013997:Time Factors
D013936:Thymidine