title: Thermotropic transition in the states of proteins in sarcoplasmic reticulum vesicles.
abstract: Interaction between protein and phospholipid molecules in sarcoplasmic reticulum (SR) was studied by measurement of the conformational fluctuation of the protein using the kinetics of the hydrogen-deuterium exchange reaction. It was revealed that the state of SR ATPase undergoes a phase transition at about 18 degrees C with boundary lipids, corresponding to a discrete change in the activation energy of the Ca2+, Mg2+-ATPase reaction.
mesh_terms: D000818:Animals; D003903:Deuterium; D007553:Isotope Labeling; D007700:Kinetics; D009124:Muscle Proteins; D010743:Phospholipids; D011487:Protein Conformation; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D013696:Temperature
chemical_list: D009124:Muscle Proteins; D010743:Phospholipids; D003903:Deuterium