On the formation of thromboxane B2 and 12l-hydroxy-5,8,10,14-eicosatetraenoic acid (12 ho-20:4) in tissues from the guinea pig.
Homogenates of tissues from the guinea pig were incubated at 37 degrees C for 30 min and the amounts of thromboxane B2 and 12L-hydroxy-5,8,10,14-eicostetraenoic acid (12ho-2-:4) formed from endogenous arachidonic acid were determined by multiple-ion analysis. Lung and spleen homogenates produced 7146 +/- 500 ng/g and 3046 +/- 542 ng/g, respectively, of thromboxane B2 and 15 122 +/- 3536 ng/g and 29 516 +/- 1966 ng/g, respectively, of 12ho-20:4. The amounts of both compounds in homogenates of kidney, stomach, liver, heart and whole blood were at least 10-times lower than those found in lung and spleen. 5,8,11,14-Eicostetraynoic acid and nordihydroguaiaretic acid inhibited 12ho-20:4 formation in spleen homogenates whereas indomethacin and arachidonic acid stimulated it.
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000818:Animals; D001095:Arachidonic Acids / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000494:pharmacology; D002396:Catechols / Q000494:pharmacology; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006168:Guinea Pigs; D006880:Hydroxy Acids / Q000096:biosynthesis*; D007213:Indomethacin / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D011714:Pyrans / Q000096:biosynthesis*; D013154:Spleen / Q000187:drug effects / Q000378:metabolism
D001095:Arachidonic Acids; D002396:Catechols; D006880:Hydroxy Acids; D011714:Pyrans; D015117:5,8,11,14-Eicosatetraynoic Acid; D007213:Indomethacin