Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells.
Micrococcal nuclease was used to eliminate endogenous protein synthesis in extracts prepared from L cells. The nuclease can be inhibited subsequently with 2'-deoxythymidine-3', 5'-diphosphate. Nuclease-treated extracts primed with exogenous reovirus mRNA, synthesized full length polypeptides with linear kinetics for almost two hours leading to stimulation of the order of 10(4) times over endogenous background. On the average, between 40 and 50 molecules of polypeptide were synthesized per molecule of mRNA.
D000818:Animals; D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D005914:Globins / Q000096:biosynthesis; D007700:Kinetics; D007739:L Cells / Q000378:metabolism*; D008836:Micrococcal Nuclease; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D012333:RNA, Messenger; D001312:Aurintricarboxylic Acid; D005914:Globins; D008836:Micrococcal Nuclease