title: Growth of rubella virus in primary rabbit kidney cell cultures maintained with and without calf serum.
abstract: Rubella virus growth was tested in primary rabbit kidney cell cultures, maintained with or without calf serum. In fluid phase of the cultures maintained with 1.5% of heat-inactivated calf serum, the infectious virus titers were much higher than in the system without serum. The differences reached 1.5-2.0 log10 TCID50/ml. Virus multiplication curves also ran different courses. In serum-containing system virus titer increased up to the 9th day post infection, while without serum--only up to 4-5 days. The time of appearance and the type of cytopathic effect were not affected by the absence of serum or by its presence in a concentration of 1.5%.
mesh_terms: D000818:Animals; D001769:Blood; D002417:Cattle; D003470:Culture Media; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D006358:Hot Temperature; D007668:Kidney; D011817:Rabbits; D012411:Rubella Vaccine; D012412:Rubella virus / Q000254:growth & development* / Q000302:isolation & purification; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
chemical_list: D003470:Culture Media; D012411:Rubella Vaccine