[Radiometric method of determining the phagocytic activity of macrophages in vitro].
The authors describe a radiometric method for determination of phagocytic activity of macrophages, using as an object for phaging hen erythrocytes labeled with 51Cr. The basic principle consists of quantitative determination of phaged cells by means of measuring the activity, found in the phagocytes. The method is described in details and the obtained results in normal mice and such with experimental leucosis NKLI are compared with those, checked by the classical methods for determining phagocytic activity in vivo and in vitro. On the basis of these data the authors indicate the advantage of the method, prepared by them, because it alows objective checking of phagocytosis on a large number of cells and thus increases the possibilities of obtaining significant results.
D000818:Animals; D002645:Chickens / Q000276:immunology; D002860:Chromium Radioisotopes; D004912:Erythrocytes / Q000276:immunology; D066298:In Vitro Techniques; D007942:Leukemia, Experimental / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008722:Methods; D051379:Mice; D010587:Phagocytosis; D011874:Radiometry
D002860:Chromium Radioisotopes
Radiometrichen netod za opredeliane fagotsitnata aktivnost na makrofagi in vitro.