In vitro fertilization of mouse and hamster eggs after freezing and thawing.
Mouse eggs (12-14%) and hamster eggs (87-94%) appear normal upon thawing after having been stored for 30 min to 25 h at -70 degrees C and -196 degrees C. While 89-98% of the normal appearing hamster eggs are capable of fertilization in vitro, only 4-13% of the mouse eggs can be fertilized. The ability of fertilized eggs to develop in culture and their viability upon transfer to pseudopregnant recipients is under investigation.
D000818:Animals; D006224:Cricetinae / Q000502:physiology*; D005260:Female; D005306:Fertilization; D005615:Freezing; D066298:In Vitro Techniques; D008297:Male; D051379:Mice / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D014021:Tissue Preservation