Measurement of glucose in plasma, with use of immobilized glucose oxidase and peroxidase.
We describe a novel fluorometric technique for simple, rapid, and economical assay of glucose by use of immobilized glucose oxidase and peroxidase. A cylindrical magnetic stirrer was specially designed to hold the immobilized enzymes firmly and to allow the reaction minzymic transformation quickly. Blood plasma can be assayed directly with no pretreatment. Ascrobic acid, uric acid, and creatinine in concenctrations of 0.25, 0.25, and 0.2 g/liter, respectively, did not interfere. The linearity of the assay was extended to 4.0 g of glucose concentrations. Results by our technique and by the otoluidine or hexokinase methods agreed well. The immobilized enzymes are stable for several months and can be used for several hundred highly accurate and reproducible assays.
D001234:Aspergillus niger / Q000201:enzymology; D001322:Autoanalysis; D001786:Blood Glucose / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005949:Glucose Oxidase / Q000378:metabolism; D006719:Homovanillic Acid / Q000494:pharmacology; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008722:Methods; D010544:Peroxidases / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D001786:Blood Glucose; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D010544:Peroxidases; D006719:Homovanillic Acid