Intercellular adhesive selectivity. I. An improved assay for the measurement of embryonic chick intercellular adhesion (liver and other tissues).
An improved assay for measuring intercellular adhesive selectivity of embryonic chick liver cells is described. Three major improvements over earlier procedures are noted: (a) enhanced reproducibility of liver cell-liver cell aggregate adhesion (homotypic adhesion) was achieved; (b) 25-70% of the input cells adhered to the collecting aggregates during the course of routine experiments as compared to the 0.25% in earlier assays. This increase in cellular adhesion suggests that the observed cell pick-up is a characteristic of the majority of the dissociated liver cell population; (c) the rate of intercellular adhesion was increased 1,000-fold. The main feature of the assay is that it measures the tissue adhesive selectivities of the dissociated cell population. Studies were undertaken on three embryonic chick tissues (liver, neural retina, and mesencephalon) to determine the tissue selectivity of intercellular adhesion of these dissociated cell types. Some general properties of liver cell homotypic adhesion have been studied and are reported.
D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion; D002449:Cell Aggregation; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D006821:Hyaluronoglucosaminidase; D007700:Kinetics; D008099:Liver / Q000502:physiology*; D009899:Optic Lobe, Nonmammalian / Q000502:physiology; D009928:Organ Specificity; D012160:Retina / Q000502:physiology; D013997:Time Factors; D014357:Trypsin
D003470:Culture Media; D006821:Hyaluronoglucosaminidase; D014357:Trypsin