The use of ninhydrin as a reagent for the reversible modification of arginine residues in proteins.
A simple technique was developed for the specific reversible modification of guanidino groups in proteins involving reaction with ninhydrin. The extent of the reaction is easily determined non-destructively by spectrophotometric analysis. The reagent can also be used for the titration of sterically unhindered thiol groups in proteins.
D001120:Arginine; D003545:Cysteine; D007192:Indenes / Q000494:pharmacology; D007700:Kinetics; D009555:Ninhydrin / Q000494:pharmacology; D010206:Papain; D013053:Spectrophotometry; D014357:Trypsin; D014361:Trypsin Inhibitors
D007192:Indenes; D014361:Trypsin Inhibitors; D001120:Arginine; D014357:Trypsin; D010206:Papain; D009555:Ninhydrin; D003545:Cysteine