Removal of hepatitis B surface antigen (HBsAg) from plasma fractions.
Endogenous or deliberately added hepatitis B antigen was removed and concentrated for assay from albumin, and from coagulation factor II, VII, IX, and X concentrates as model plasma fractions. The concentrates carry considerable risk of causing hepatitis in transfused patients. The amount of antigen remaining in the fraction was estimated to be less than 1/10,000 of that detectable by the Ausria II radioimmunoassay and 1/100 of that found to be infectious when highly contaminated human sera were diluted and injected in chimpanzees. Batch fractionation methods with polyethylene glycol were used. The yield of albumin was 96 per cent and of the coagulation factors about 90 per cent.
D001798:Blood Proteins / Q000032:analysis*; D001803:Blood Transfusion; D011232:Chemical Precipitation; D005164:Factor IX / Q000032:analysis; D005167:Factor VII / Q000032:analysis; D005170:Factor X / Q000032:analysis; D005374:Filtration; D006509:Hepatitis B / Q000097:blood*; D006511:Hepatitis B Antigens / Q000302:isolation & purification*; D006801:Humans; D011092:Polyethylene Glycols / Q000494:pharmacology; D011516:Prothrombin / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis
D001798:Blood Proteins; D006511:Hepatitis B Antigens; D012709:Serum Albumin; D011092:Polyethylene Glycols; D005167:Factor VII; D011516:Prothrombin; D005164:Factor IX; D005170:Factor X