title: Quantitative assay of sulphinpyrazone in plasma and urine by high-performance liquid chromatography.
abstract: A method for the quantitative determination of sulphinpyrazone in plasma and urine is described. The drug is extracted from the acidified aqueous phase with 1-chlorobutane-ethylene dichloride (4:1) and separated from its metabolites by high-performance liquid chromatography on 5-mum LiChrosorb using dichloromethane-ethanol-water-acetic acid (79.1:19:1.9:0.002) as the mobile phase. The sensitivity limit is 0,2mug/ml using a 1-ml sample. Examples of applications are given.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008722:Methods; D012997:Solvents; D013442:Sulfinpyrazone / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D012997:Solvents; D013442:Sulfinpyrazone