[Direct Quantitative Evaluation of Thin-Layer Chromatograms by Measuring Remission and Fluorescence (author's transl)].
A highly specific method for the estimation of free serotonin in urine is described. The technique is based on the separation of the serotonin from urine by an ion exchange resin, acetylation of the separated serotonin with subsequent extraction into an organic solvent followed by a thin-layer chromatographic separation. After t.l.c. the plates are sprayed with o-phthaldialdehyde (OPT), whereupon serotonin forms intense fluorescent spots, which can be measured directly. The reproducibily of this method is about +/- 7%.
D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer / Q000379:methods*; D005453:Fluorescence; D008722:Methods; D012701:Serotonin / Q000652:urine*
D012701:Serotonin
Die direkte quantitative Auswertung von Dünnschichtchromatogrammen durch Remissions- und Fluoreszenzmessungen