Influence of valine deprivation and its reversal on fatty acid metabolism in HeLa cells.
The effects that amino acid starvation and re-supplementation have on fatty acid metabolism in HeLa cells have been studied using radio gas chromatographic techniques. Deprivation of valine for 13.5 h caused fatty acid de novo biosynthesis, elongation and desaturation to cease. This effect was reversed within 5 h by adding valine back to the culture. During deprivation accumulation of triacylglycerol occurred. The return of valine to the culture caused compositional changes in the triacylglycerols and phosphatidylcholines.
D002849:Chromatography, Gas; D005227:Fatty Acids / Q000378:metabolism*; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000096:biosynthesis; D014280:Triglycerides / Q000096:biosynthesis; D014633:Valine / Q000378:metabolism / Q000494:pharmacology*
D005227:Fatty Acids; D010713:Phosphatidylcholines; D014280:Triglycerides; D014633:Valine