[Method of parallel study of cell metabolism and extracellular structures using oncornavirus as a model].
A method is described which permits simultaneous comparative study of cell metabolism and extracellular virus structures in minimal volumes. The method is based on the growing of cells in vials for scintillation count and it has been tested on a HEp-2 cell culture chronically producing oncornavirus type D. A comparative analysis of acid-insoluble radioactivity in heterogenous systems has been shown to be justified. For demonstration of the fields of possible utilization of the method, data are presented from the studies of the effect of various inhibitors (ethidium bromide, chloramphenicol, cycloheximide and anti-PPLO agent) on 3H-uridine incorporation into cells and extracellular virus structures.
D000890:Anti-Infective Agents / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D002460:Cell Line; D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D009858:Oncogenic Viruses / Q000187:drug effects / Q000254:growth & development*; D012367:RNA, Viral / Q000096:biosynthesis*; D014779:Virus Replication
D000890:Anti-Infective Agents; D000998:Antiviral Agents; D012367:RNA, Viral; D002701:Chloramphenicol; D003513:Cycloheximide; D004996:Ethidium
Metod parallel'nogo izucheniia kletochnogo metabolizma i vnekletochnykh struktur na modeli onkornavirusa