Theophylline radioimmunoassay: synthesis of antigen and characterization of antiserum.
Antisera to theophylline (T) have been obtained by immunizing rabbits with a conjugate of 8-(3-carboxypropyl)-1,3-dimethylxanthine and bovine serum albumin. Comparison of 50% displacement values indicated good selectivity for T vs. a number of other xanthine derivatives. An analytical procedure using this antiserum can measure 200 pg of T and direct analysis of 0.1 mug/ml in plasma or 0.02 mug/ml in saliva is feasible.
D000818:Animals; D000917:Antibody Formation; D002110:Caffeine; D003429:Cross Reactions; D005260:Female; D007106:Immune Sera; D008297:Male; D008832:Microchemistry; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D012463:Saliva / Q000032:analysis; D012710:Serum Albumin, Bovine; D013805:Theobromine; D013806:Theophylline / Q000032:analysis* / Q000097:blood / Q000276:immunology
D007106:Immune Sera; D012710:Serum Albumin, Bovine; D002110:Caffeine; D013806:Theophylline; D013805:Theobromine