The effects of mitotic inhibitors on the structure of vinblastine-induced tubulin paracrystals from sea-urchin eggs.
Vinblastine sulphate (VLB) is known to induce in vivo formation of tubulin paracrystals in sea-urchin eggs. Corresponding paracrystals have been prepared in the presence of both vinblastine sulphate and other mitoclasic agents. Careful standardization of conditions was required to restrict the formation of alternative forms of the paracrystals induced by vinblastine alone. Comparisons were made between preparations in terms of paracrystal shape, size, proportion of eggs containing paracrystals, number per egg and their relative times of first appearance. A correlation between such properties were established. Comparison of paracrystals at the ultrastructural level showed them all to be similar regardless of the drugs present during their formation. The implications of tubulin polymerization in the presence of mitoclasic agents are discussed and mechanisms for paracrystal enhancement by combinations of such drugs are suggested. Some similarities of paracrystal and microtubule seeding are discussed together with the activation of tubulin in the pool.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002219:Carbamates / Q000494:pharmacology*; D003078:Colchicine / Q000494:pharmacology*; D003460:Crystallization; D005260:Female; D006023:Glycoproteins; D006118:Griseofulvin / Q000494:pharmacology*; D010063:Ovum / Q000187:drug effects / Q000648:ultrastructure*; D011033:Podophyllin / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D012617:Sea Urchins; D014404:Tubulin; D014747:Vinblastine / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D002219:Carbamates; D006023:Glycoproteins; D014404:Tubulin; D006118:Griseofulvin; D014747:Vinblastine; D011033:Podophyllin; D003078:Colchicine