Globin synthesis in uraemia.
Globin synthesis was measured by incubating washed packed red cells with a balanced amino acid mixture and 14C-Histidine. After globin had been isolated from the haemolysate the 14C-incorporation rate per 10 mg globin per min was estimated. With regard to globin synthesis no differences existed between patients on chronic intermittent haemodialysis therapy (n equals 41), patitents suffering from secondary anaemia due to chronic infection, rheumatism or malignant diseases (n equals 21) and healthy subjects (n equals 37). Patients with acute bleeding on the other hand, had significant higher 14C-incorporation rates caused by an increase of the reticulocyte count mu1 blood (n equals 13). When substances retained in renal failure were added to the incubation mixture a marked inhibition took place with guanidinosuccinic acid, methylguanidine and fraction containing peptides with a molecular weight between 1200-1400 present in uraemic serum and normal urine, but not in serum from patients without renal failure.
D000596:Amino Acids / Q000378:metabolism; D005914:Globins / Q000096:biosynthesis*; D006639:Histidine / Q000378:metabolism; D006801:Humans; D012156:Reticulocytes / Q000378:metabolism; D014511:Uremia / Q000378:metabolism*
D000596:Amino Acids; D006639:Histidine; D005914:Globins