Teratogenic and toxicological examination of 2,4,5-trichlorphenoxyacetic acid in developing chick embryos.
Practical grade 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), dissolved in dimethylsulfoxide (DMSO), was injected into the air space of fertilized chicken eggs prior to incubation. Doses of 2,4,5-T administered were 12.5, 25, 50, 75, 100, and 125 mg/kg to determine herbicide toxicity on the first day of incubation. A similar group was studied on day 5 of incubation with doses of 2,4,5-T at 50, 75, 100 and 250 mg/kg. LD50 was estimated to be 62 mg/kg on day zero and 68 mg/kg on day 5. Additionally, embryos were exposed to 2,4,5-T at 50 mg/kg on day zero of gestation and sacrificed after 48 h of incubation. Serial sections were examined for teratological and developmental anomalies. None were found.
D015085:2,4,5-Trichlorophenoxyacetic Acid / Q000494:pharmacology / Q000633:toxicity*; D000014:Abnormalities, Drug-Induced; D000818:Animals; D002642:Chick Embryo; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D005298:Fertility / Q000187:drug effects; D007928:Lethal Dose 50; D013723:Teratogens; D013997:Time Factors
D013723:Teratogens; D015085:2,4,5-Trichlorophenoxyacetic Acid; D004121:Dimethyl Sulfoxide