The influence of adenine nucleotides and oxidizable substrates on triethyltin-mediated chloride uptake by rat liver mitochondria in potassium chloride media.
In a 100 mM-KCl medium, pH 6.8, containing ATP increasing concentrations of triethyltin cause an uptake of Cl- into mitochondria with a maximum at 1 muM. This can be inhibited by atractylate or oligomycin, but is virtually unaffected by the presence of rotenone. When the medium contains substrate (pyruvate, beta-hydroxybutyrate or succinate), both in the presence and absence of adenine nucleotides, Cl- uptake is greater with a maximum at 1-10 muM-triethyltin. If substrate oxidation is blocked by respiratory-chain inhibitors the Cl- uptake mediated by triethyltin is inhibited except in the media containing ATP, when the characteristics of Cl- uptake similar to that found in the medium containing ATP alone are observed. Under all conditions tested Cl- uptake is decreased by the presence of 2,4-dinitrophenol. It is concluded that energy from either the oxidation of substrate or the hydrolysis of ATP is associated with the generation of sufficient OH- to enable the triethyltin-mediated Cl-/OH- exchange to occur under the metabolic conditions relevant to this action of triethyltin.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001278:Atractyloside / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism*; D004140:Dinitrophenols / Q000494:pharmacology; D006878:Hydroxides / Q000378:metabolism; D006885:Hydroxybutyrates; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology; D011189:Potassium Chloride; D011773:Pyruvates; D051381:Rats; D012402:Rotenone / Q000494:pharmacology; D013386:Succinates; D014220:Trialkyltin Compounds / Q000494:pharmacology*; D014267:Triethyltin Compounds / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D002712:Chlorides; D004140:Dinitrophenols; D006878:Hydroxides; D006885:Hydroxybutyrates; D009840:Oligomycins; D011773:Pyruvates; D013386:Succinates; D014220:Trialkyltin Compounds; D014267:Triethyltin Compounds; D012402:Rotenone; D001278:Atractyloside; D000968:Antimycin A; D011189:Potassium Chloride; D000255:Adenosine Triphosphate