Reovirus-specific polypeptides: analysis using discontinuous gel electrophoresis.
The electrophoretic analysis of reovirus-specific polypeptides in infected cells using a discontinuous gel system has allowed the resolution of additional viral-specific polypeptides, including one large-sized gamma3 and two (or possibly three) medium-sized (mu3, mu4, mu5(?)) species. The proteins designated mu0, sigma1, and sigma2 based on electrophoretic mobility in gel systems containing phosphate-urea correspond to mu4, sigma2, and sigma1, respectively, when analyzed in systems containing Tris-glycine. It is likely that protein modifications (phosphorylation and glycosylation) are responsible for at least some of these differences.
D000096:Acetone; D002021:Buffers; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005998:Glycine; D007106:Immune Sera; D007739:L Cells; D012089:Mammalian orthoreovirus 3 / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D010710:Phosphates; D012087:Reoviridae / Q000032:analysis*; D014325:Tromethamine; D014508:Urea; D014764:Viral Proteins / Q000032:analysis*
D002021:Buffers; D007106:Immune Sera; D010455:Peptides; D010710:Phosphates; D014764:Viral Proteins; D014325:Tromethamine; D000096:Acetone; D014508:Urea; D005998:Glycine