Compartmentation and control of arginine metabolism in Neurospora.
The fate of [14-C]arginine derived from the medium or from biosynthesis has been examined in Neurospora growing in arginine-supplemented medium. In both cases the label enters the cytosol, where it is used efficiently for both protein synthesis and catabolism before mixing with the majority of the endogenous [12C]arginine pool. Both metabolic processes appear to use the same cytosolic arginine pool. It is calculated that the nonorganellar cytoplasm contains approximately 20% of the intracellular arginine pool when the cells are growing in arginine-supplemented medium. The results suggest that compartmentation of arginine is a significant factor in controlling arginine metabolism in Neurospora. The significance of these results for studies of amino acid metabolism in other eukaryotic systems is discussed.
D001120:Arginine / Q000378:metabolism*; D002245:Carbon Dioxide / Q000096:biosynthesis; D003600:Cytosol / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis; D009154:Mutation; D009491:Neurospora / Q000378:metabolism*; D009952:Ornithine / Q000096:biosynthesis; D014508:Urea / Q000096:biosynthesis
D005656:Fungal Proteins; D002245:Carbon Dioxide; D014508:Urea; D001120:Arginine; D009952:Ornithine