The use of tetrahydrocortisone-3-beta-D-glucuronide in the measurement of urinary 17-hydroxycorticosteroids.
THE 3-G was added to H2O and 56 urines, and the recovered THE was measured by the Porter-Silber method. The recovery from H2O was quantitative (98 +/- 2%), but highly variables from urine, ranging from 35 to 100%. The necessity of the proper standard in analysis of urinary steroid glucuronides was demonstrated. The presence in urine of endogenous inhibitors to beta-glucuronidase was confirmed.
D015065:17-Hydroxycorticosteroids / Q000652:urine*; D003124:Colorimetry; D005260:Female; D005965:Glucuronates / Q000494:pharmacology; D005966:Glucuronidase / Q000037:antagonists & inhibitors; D006801:Humans; D008297:Male; D013761:Tetrahydrocortisone / Q000494:pharmacology
D015065:17-Hydroxycorticosteroids; D005965:Glucuronates; D013761:Tetrahydrocortisone; D005966:Glucuronidase