The effects of hydroquinone, hematin and heme-containing proteins on prostaglandin biosynthesis by methylcholanthrene-transformed mouse BALB/3T3 fibroblasts.
Cultured methylcholanthrene-transformed 3T3 mouse fibroblasts secrete large amounts of prostaglandin into the medium. Prostaglandin biosynthesis by these cells can be stimulated by arachidonic acid and, to a much lesser extent, by glutathione and porphyrin. In the presence of hydroquinone, prostaglandin production is increased; the most striking increases are seen  at concentrations that are lethal. Porphyrins, when added to cells in the presence of lethal levels of hydroquinone, inhibit stimulation of prostaglandin E2 synthesis but stimulate the production of prostaglandin F2alpha.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology; D006418:Heme / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006420:Hemeproteins / Q000494:pharmacology*; D006427:Hemin / Q000378:metabolism; D006454:Hemoglobins / Q000494:pharmacology; D006873:Hydroquinones / Q000494:pharmacology*; D007213:Indomethacin / Q000494:pharmacology; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D011166:Porphyrins / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis*; D013268:Stimulation, Chemical
D001095:Arachidonic Acids; D006420:Hemeproteins; D006454:Hemoglobins; D006873:Hydroquinones; D011166:Porphyrins; D011453:Prostaglandins; D006418:Heme; D008748:Methylcholanthrene; D006427:Hemin; D005978:Glutathione; D007213:Indomethacin