Iodothyronine content of human thyroid albumin.
Stable thyroid hormones (T4 and T3)1) have been demonstrated in pure albumin isolated from normal human thyroid tissue iodinated in vivo. Five samples of albumin were separated from other thyroid proteins by acrylamide gel electrophoresis. After pronase hydrolysis, the content of Thyroid hormones was measured chemically (T4 + T3) as well as by competitive radioactive measurement (T4) and radioimmunoassay (T3). The purity of the albumin and validity of these measurements were confirmed by different techniques. The synthesis of thyroid hormones is not therefore a property unique to Tg and may occur in albumin. However the amount of iodothyronines in the albumin (average 0.004 residue per molecule) is much less than that found in Tg (0.5 residue per molecule). In the albumin as in Tg the number of hormone residues per molecule is proportional to the number of atoms of iodine. At an equivalent iodine concentration, the albumin seems capable of forming the thyroid hormones as well as Tg. The difference between these two proteins, in their capacity to synthesize thyroid hormones, seems to depend on their capacity for iodination. This difference of iodination does not seem to be linked with the number of tyrosyl residues, but might be related to the position of these residues.
D000418:Albumins / Q000032:analysis*; D004105:Diiodotyrosine / Q000032:analysis; D006801:Humans; D007455:Iodine / Q000097:blood; D007470:Monoiodotyrosine / Q000032:analysis; D011485:Protein Binding; D013954:Thyroglobulin / Q000032:analysis*; D013963:Thyroid Hormones / Q000032:analysis*; D013970:Thyronines / Q000032:analysis*; D013974:Thyroxine / Q000032:analysis; D014284:Triiodothyronine / Q000032:analysis
D000418:Albumins; D013963:Thyroid Hormones; D013970:Thyronines; D014284:Triiodothyronine; D004105:Diiodotyrosine; D013954:Thyroglobulin; D007455:Iodine; D007470:Monoiodotyrosine; D013974:Thyroxine