title: Formyltetrahydrofolate synthetase-catalyzed formation of ATP from carbamyl phosphate and ADP. Evidence for a formyl phosphate intermediate in the enzyme's catalytic mechanism.
abstract: Formyltetrahydrofolate synthetase from Clostridium cylindrosporum catalyzes phosphoryl transfer from carbamyl phosphate in ADP to form ATP. The phosphoryl transfer reaction has an obligatory requirement for tetrahydrofolate presumably as a cofactor for a proper conformation of the active site. Carbamyl phosphate is an analog of formyl phosphate-a potential intermediate in the normal enzymic reaction. The ability of the enzyme to promote synthesis of ATP from carbamyl phosphate and ADP supports a stepwise chemical reaction mechanism for the enzyme in which formyl phosphate participates as a tightly bound intermediate.
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D002221:Carbamyl Phosphate / Q000378:metabolism; D003013:Clostridium / Q000201:enzymology*; D005574:Formate-Tetrahydrofolate Ligase / Q000378:metabolism*; D005561:Formates / Q000378:metabolism; D007700:Kinetics; D008025:Ligases / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism; D013997:Time Factors
chemical_list: D005561:Formates; D009943:Organophosphorus Compounds; D002221:Carbamyl Phosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D008025:Ligases; D005574:Formate-Tetrahydrofolate Ligase