The androgenic regulation of prostate proteins with a high affinity for deoxyribonucleic acid. Evidence for a prostate deoxyribonucleic acid-unwinding protein.
1. When testosterone is injected into castrated rats in vivo, a significant increase in the incorporation of [35S]methionine into prostate proteins may be detected under conditions in vitro. 2. Studies based on DNA-cellulose chromatography show that the synthesis of prostate proteins with a high affinity for DNA is particularly enhanced by androgenic stimulation. 3. These changes in protein synthesis are negated when the anti-androgen, cyproterone acetate, is administered concomitantly with testosterone in vivo. 4. Two assays were developed for measuring the strand separation of prostate DNA; first, the retention of 3H-labelled native DNA on nitrocellulose membranes, and second, the activation of native DNA as a template for 9S prostate DNA polymerase. On the basis of these criteria, DNA-unwinding activity is present in the prostate gland and it is regulated by androgens in a steroid-and tissue-specific manner. 5. The results are discussed in the context of the mechanism of action of androgens, particularly since the changes provoked in DNA-unwinding activity by androgens precede the onset of DNA replication and mitosis.
D000818:Animals; D001665:Binding Sites; D003534:Cyproterone / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D003851:Deoxyribonucleases / Q000032:analysis; D008297:Male; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D011467:Prostate / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D013268:Stimulation, Chemical; D013698:Templates, Genetic; D013739:Testosterone / Q000494:pharmacology*
D013739:Testosterone; D004247:DNA; D008715:Methionine; D003534:Cyproterone; D004254:DNA Nucleotidyltransferases; D003851:Deoxyribonucleases