Characterization of the synthesized collagen by mouse L-929 (L-5) cells.
In 48 h incubation L-929 (L-5) cells secreted collagenous protein accounting for 16% of the total protein. CM-chromatography, SDS-acrylamide gel electrophoresis and DEAE-cellulose chromatography revealed that the collagenous protein was mainly composed of type I collagen, having no collagen of type III. In addition, precursor-form collagen and tropocollagen molecules were found to be present in the incubate.
D000478:Alkylation; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D003094:Collagen / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D007739:L Cells / Q000378:metabolism*; D051379:Mice; D011817:Rabbits
D003094:Collagen