The influence of oxygen deficiency and electrical stimulation on the concentrations of ATP, ADP, AMP and phosphotaurocyamine in the body-wall musculature of Arenicola marina.
1) Concentrations of ATP, ADP, and AMP, as well as phosphotaurocyamine in the body-wall musculature of Arenicola marina were determined enzymatically and by means of isotachophoresis after 12, 24, and 48 h of experimental anaerobiosis and after electrical stimulation under aerobic and anaerobic conditions. 2) Freshly collected lugworms contained 3.5-3.8 mumol ATP, 0.8-1.0 mumol ADP, and 0.3-0.5 mumol AMP, as well as 4.5-4.7 mumol phosphotaurocyamine per g wet weight (energy charge 0.81-0.85). 3) After 48 h of anaerobiosis the concentration of ATP was decreased to one half, that of phosphotaurocyamine to one third of the initial value, while the content of ADP on the other hand nearly doubled and that of AMP trebled (energy charge 0.60-0.63). 4) Electrical stimulation in the absence of oxygen caused a rapid decrease of the ATP and phosphotaurocyamine to low levels corresponding to those found after long-term anaerobiosis; the ADP and AMP concentrations were slightly higher than those found after long-term anaerobiosis (energy charge 0.55-0.58).
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000693:Anaerobiosis; D000818:Animals; D004558:Electric Stimulation; D007700:Kinetics; D009132:Muscles / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism; D011077:Polychaeta; D013654:Taurine / Q000031:analogs & derivatives* / Q000378:metabolism
D009943:Organophosphorus Compounds; D013654:Taurine; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate