Formation of uridine diphosphate 2-deoxy-D-glucose and guanosine diphosphate 2-deoxy-D-glucose in vitro using animal enzymes.
The enzymic formation of guanosine diphosphate 2-deoxy-D-glucose and uridine diphosphate 2-deoxy-D-glucose from synthetically prepared 2-deoxy-D-glucose 1-phosphate is described. Incubation of 2-deoxy-D-glucose 1-phosphate with an enzyme preparation from bovine mammary glands and either GTP or UTP gives rise to the corresponding nucleoside-diphosphate derivative of 2-deoxy-D-glucose. Uridine diphosphate 2-deoxy-D-glucose could also be obtained by incubation of 2-deoxy-D-glucose 1-phosphate with UTP and UDP glucose pyrophosphorylase from beef liver.
D000818:Animals; D002417:Cattle; D003847:Deoxyglucose / Q000378:metabolism; D005260:Female; D006156:Guanosine Diphosphate Sugars / Q000031:analogs & derivatives / Q000096:biosynthesis*; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D009904:Optical Rotation; D011247:Pregnancy; D014532:Uridine Diphosphate Glucose / Q000031:analogs & derivatives / Q000096:biosynthesis*; D014539:Uridine Diphosphate Sugars / Q000096:biosynthesis*
D006156:Guanosine Diphosphate Sugars; D009702:Nucleoside Diphosphate Sugars; D014539:Uridine Diphosphate Sugars; C011836:guanosine diphosphate 2-deoxy-D-glucose; C012091:uridine diphosphate 2-deoxyglucose; D003847:Deoxyglucose; D014532:Uridine Diphosphate Glucose