[Lysosomal component in the mechanism of the toxic effect of sporofusarin].
The effect of Fusarium sporotrichiella v. sporotrichioides mycotoxin (sporofusarin) on the total and non-sedimentary supernatant activity of 13 marker-enzymes of subcellular particles (2 mitochondrial enzymes-cytochrome oxidase and malate dehydrogenase; 8 lysosomal enzymes -- acid phosphatase, acid RNAase, acid DNAase, arylsulphatases A and B, beta-N-acetylglucosaminidase, beta-glucuronidase, beta-galactosidase and beta-glucosidase; 2 microsomal enzymes -- glucose-6-phosphatase and acetylesterase; plasma membrane enzyme -- alkaline phosphatase) of the rat liver, kidney, spleen and bone-marrow was studied in in vivo experiments. The latter demonstrated that sporofusarin effects were characterized by a significant organ and organella specificity, viz. the toxin caused a sharply increased activity, mainly of lysosomes enzymes and labilization of the lysosomal membranes, primarily in the spleen and the bone-marrow. A conclusion is drawn that the discovered selective destructive action of sporofusarin on the lysosomes may be regarded as a new phenomenon that, possibly is directly related to the characterization of the mechanism responsible for a specific effect produced by sporofusarin.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D004950:Esterases / Q000378:metabolism; D005670:Fusarium; D006026:Glycoside Hydrolases / Q000378:metabolism; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D009183:Mycotoxins / Q000494:pharmacology*; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D013154:Spleen / Q000187:drug effects
D009183:Mycotoxins; D010088:Oxidoreductases; D004950:Esterases; D006026:Glycoside Hydrolases
Lizosomal'nyĭ komponent v mekhanizme toksicheskogo deĭstviia sporofuzarina