Effect of polyamines on the uptake of poly (2'-fluoro-2'-deoxyuridylic acid) by a mammalian cell line.
VERO cells can take up poly(dUfl)1 from the medium. The uptake involves surface adsorption and, most probably, intracellular penetration. Part of the poly(dUfl) is hydrolyzed during incubation with the cells but the hydrolysis products are not incorporated into de novo synthesized nucleic acids. The uptake is reduced by serum and stimulated by polycationic ionenes. The magnitude of stimulation depends on the structure of the ionene and the treatment regimen.
D001693:Biological Transport, Active; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005468:Fluorodeoxyuridylate / Q000378:metabolism*; D007700:Kinetics; D011073:Polyamines / Q000494:pharmacology*; D014500:Uracil Nucleotides / Q000378:metabolism*
D011073:Polyamines; D014500:Uracil Nucleotides; D005468:Fluorodeoxyuridylate