title: A morphological mutant of Paracoccidioides brasiliensis strain IVIC Pb9. Isolation and wall characterization.
abstract: A morphological mutant of Paracoccidioides brasiliensis strain IVIC Pb9 was isolated after treatment of the yeast-like (Y) form with nitrosoguanidine. Colonies of the mutant grown at room temperature did not show the whitish cotton-like morphology typical of the mycelial form of the parental strain. Y-cells were much smaller than those produced by the parent and grew forming chains of different sizes. The main chemical difference in the wall of the Y-form was the replacement of the alpha-I,3-glucan, typical of the parental strain, by an amorphous I,3-mannan in the mutant.
mesh_terms: D002473:Cell Wall / Q000032:analysis; D005658:Fungi / Q000166:cytology*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D008351:Mannans / Q000032:analysis; D008358:Mannose / Q000032:analysis; D008769:Methylnitronitrosoguanidine; D009153:Mutagens; D009154:Mutation; D010228:Paracoccidioides / Q000032:analysis / Q000166:cytology* / Q000302:isolation & purification; D011134:Polysaccharides / Q000032:analysis; D013696:Temperature
chemical_list: D008351:Mannans; D009153:Mutagens; D011134:Polysaccharides; D008769:Methylnitronitrosoguanidine; D005947:Glucose; D008358:Mannose; D005690:Galactose