title: Rabbit muscle pyruvate kinase. Amino- and carboxyl-terminal studies.
abstract: Amino-terminal analysis of rabbit muscle pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) failed to detect the presence of any free amino-terminal residues. Acetyl group analysis demonstrated the presence of between 3.7 and 4.0 mol of acetyl groups per mol of enzyme. The acetylated amino-terminal residue was isolated from pronase digests of the enzyme and identified as N-acetylserine. Quantitative recovery experiments indicated that all acetyl residues are found at the amino termini. Carboxyl-terminal analyses using the tritium exchange method suggested the presence of a blocked carboxyl-terminal residue, supporting previous hydrazinolysis and carboxypeptidase studies.
mesh_terms: D000085:Acetates / Q000032:analysis; D000596:Amino Acids / Q000032:analysis*; D009132:Muscles / Q000201:enzymology*; D011770:Pyruvate Kinase; D012694:Serine / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D000085:Acetates; D000596:Amino Acids; D012694:Serine; D011770:Pyruvate Kinase