Conditions for collection of serum samples for the measurement of fibrin(ogen) degradation products by radioimmunoassay of fragment E.
A number of conditions have been assessed for the collection of serum samples for the measurement of fibrin(ogen) degradation products using a radioimmunoassay for degradation fragment E, which permits precise quantitation of differences. Blood should be collected using minimal venous occlusion into glass tubes containing 10 mg/ml of epsilon amino caproic acid and allowed to clot at 4 to 20 degrees C for at least 4 hours before centrifugation. The serum may be stored at 4 or -20 degrees C. Samples from patients receiving anticoagulant therapy should be treated with 10 IU of thrombin per ml.
D000614:Aminocaproates; D000925:Anticoagulants; D001777:Blood Coagulation; D001800:Blood Specimen Collection / Q000379:methods*; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D006801:Humans; D011863:Radioimmunoassay; D013696:Temperature; D013917:Thrombin; D013997:Time Factors
D000614:Aminocaproates; D000925:Anticoagulants; D005338:Fibrin Fibrinogen Degradation Products; D013917:Thrombin