The inhibition of the conversion of testosterone into 5alpha-dihydrotestosterone in the reproductive organs of the male rat.
The inhibition of testosterone 5alpha-reductase activity by 3-oxo-4-androstene-17beta-carboxylic acid in the male reproductive organs of the rat was demonstrated in vitro. The medium for incubation of caput epididymis showed the highest concentration of 5alpha-dihydrotestosterone (5alpha-DHT) whereas the highest concentration of testosterone (T) was recorded in medium for incubation of decapsulated testis after two hours of incubation. The 3-oxo-4-androstene-17beta-carboxylic acid (1.58 X 10(-5)M) inhibited the conversion of T to 5alpha-DHT in all the organs tested (testis, caput and cauda epididymis and ventral prostate) under identical incubation conditions.
D058891:5-alpha Reductase Inhibitors; D000736:Androstenes / Q000494:pharmacology*; D000818:Animals; D013196:Dihydrotestosterone / Q000378:metabolism*; D004822:Epididymis / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D011467:Prostate / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D013737:Testis / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism*
D058891:5-alpha Reductase Inhibitors; D000736:Androstenes; D013196:Dihydrotestosterone; D013739:Testosterone; D010088:Oxidoreductases