Large-scale purification of hepatitis B surface antigen.
Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02 M sodium phosphate buffer.
D000327:Adsorption; D001769:Blood / Q000382:microbiology*; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D006511:Hepatitis B Antigens / Q000302:isolation & purification*; D006801:Humans; D006882:Hydroxyapatites; D011092:Polyethylene Glycols
D006511:Hepatitis B Antigens; D006882:Hydroxyapatites; D011092:Polyethylene Glycols