[Non-specific dye absorption studies in quantitative cytochemical reactions with alkalic fast green and heparin-alcian blue (model experiment)].
Stöchiometria of the alkalic Fast green and Heparin-alcianblue was studied in model experiment by the aid of integrating microdensitometer. The changes of the optical density in cases of Heparin-alcian-blue occurred to be proportional to the histon quantity, in cases of staining with alkalic Fast green nonspecific dye-absorption was revealed. Results of the model experiment indicate that in quantitative cytochemical studies Heparin-alcian-blue should be preferred to alkalic Fast green.
D000423:Alcian Blue; D000818:Animals; D002417:Cattle; D004396:Coloring Agents; D003720:Densitometry; D006493:Heparin; D006651:Histocytochemistry / Q000379:methods*; D006657:Histones / Q000032:analysis*; D013950:Thymus Gland / Q000032:analysis
D004396:Coloring Agents; D006657:Histones; D006493:Heparin; D000423:Alcian Blue
Aspecifikus festék-absorptio vizsgálata alkáliás Fast green és Heparin-alciánkék kvantitatív citokémiai reakciókban