[Study of the specific anti-influenzal activity of human respiratory tract secretions and their fractions isolated by means of gel filtration].
It was found that in gel filtration of a mixture of the human nasal cavity and salivary gland secretions on Sephadex G-200, three protein peaks were regularly releaved. According to the results of disk electrophoresis, the original secretion mixture consists of 11--13 fractions. The material of peak No. 1 is found in one di-fuse band on the cathode end of gel. Peak No. 2 contains 5--7 fractions and eluate No. 3 contains 3 fractions. The gel precipitation test with monospecific rabbit antisera to human IgM, IgG and IgA demonstrated IgA to be present in the first peak.
D000918:Antibody Specificity; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification*; D007071:Immunoglobulin A, Secretory / Q000302:isolation & purification*; D009980:Influenza A virus / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology*; D009297:Nasal Mucosa / Q000276:immunology; D012137:Respiratory System / Q000276:immunology*; D012463:Saliva / Q000276:immunology
D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory
Izuchenie spetsificheskoĭ protivogrippoznoĭ aktivnosti sekretov respiratornogo trakta cheloveka i ikh fraktsi, vydelennykh metodom gel'-fil'tratsii.