On the significance of the carbohydrate moieties of bovine prothrombin for clotting activity.
Purified bovine prothrombin has been treated with different mixtures of glycosidases. Upon incubation of the prothrombin for 30 h with a combination of neuraminidase, alpha- and beta-galactosidase and beta-N-acetylglucosaminidase in 4 mM diisopropylfluorophosphate at pH 5.3 and 30 degrees C, about 70% of the carbohydrates were removed without affecting the coagulation activity. All the sialic acid and about half of the mannose, galactose and glucosamine residues were removed by this treatment.
D000117:Acetylglucosamine / Q000032:analysis; D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D001780:Blood Coagulation Tests; D002417:Cattle; D005690:Galactose / Q000032:analysis; D005696:Galactosidases / Q000378:metabolism; D008358:Mannose / Q000032:analysis; D009439:Neuraminidase / Q000378:metabolism; D011516:Prothrombin; D012794:Sialic Acids / Q000032:analysis; D013329:Structure-Activity Relationship
D012794:Sialic Acids; D011516:Prothrombin; D005696:Galactosidases; D009439:Neuraminidase; D000118:Acetylglucosaminidase; D008358:Mannose; D000117:Acetylglucosamine; D005690:Galactose