Partial purification of L-ascorbate:ferricytochrome b5 oxidoreductase from rat liver microsomes.
L-Ascorbate:ferricytochrome b5 oxidoreductase from rat liver microsomes was enriched about 750-fold after treatment with a non-ionic detergent with a yield of about 15%. The enriched enzyme still shows four bands in sodium dodecylsulfate polyacrylamide-gel electrophoresis and is a hydrophobic membrane protein.
D000818:Animals; D001205:Ascorbic Acid; D003580:Cytochromes; D004789:Enzyme Activation; D007700:Kinetics; D008055:Lipids / Q000494:pharmacology; D008297:Male; D008823:Micelles; D008862:Microsomes, Liver / Q000201:enzymology*; D008970:Molecular Weight; D010088:Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats
D003580:Cytochromes; D008055:Lipids; D008823:Micelles; D010088:Oxidoreductases; D001205:Ascorbic Acid