title: High-voltage electron microscopy of extracellular fibrillogenesis.
abstract: High-voltage electron microscopy was employed to observe developing extracellular connective tissue elements in the cervical perinotochordal and perivertebral regions in the chick embryo from 2 through 15 days' incubation. During days 2 and 3, small (10 nm) and large (18-20 nm) microfibrils surrounded the notochord, becoming evident around fibroblast-like cells in day 4. Amorphous material, globular granules and microfibrillar bundles were present at this time. Microfibrillar length increased as did the total population of microfibrils. At four days microfibrils 3-5 nm in diameter arose in all directions from globular granules. During day 9 and thereafter to day 15, microfibrillar diameters increased. This growth formed unit collagenous fibrils 30 nm in diameter or greater. Axial periodicity became evident at day 14. Small microfibrils appear to be composed largely of glycoproteins and do not contain a significant amount of collagen. The globular granules and associated filaments are probably proteoglycans. The amorphous material is believed to provide molecular collagen to developing fibrils. Large microfibrils and unit collagenous fibrils contain significant amounts of molecular collagen.
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000254:growth & development*; D003094:Collagen; D003238:Connective Tissue / Q000648:ultrastructure*; D005110:Extracellular Space / Q000648:ultrastructure; D006023:Glycoproteins; D008854:Microscopy, Electron
chemical_list: D006023:Glycoproteins; D003094:Collagen