Strain improvement in Streptomyces galilaeus, a producer of anthracycline antibiotics galirubins.
The production of epsilon-pyrromycinone glycosides in Streptomyces galilaeus increased 12-fold, with respect to the wild strain, as a result of a sequential procedure including both natural selection and treatment with mutagens (nitrous acid, UV light and gamma-irradiation). Nitrous acid exhibited the highest mutagenic effect, both in increasing the productivity and in inducing blocked mutants. A mutant strain blocked in the biosynthesis of glycosides and accumulating free epsilon-pyrromycinone as the principal metabolite was obtained.
D000880:Anthraquinones / Q000096:biosynthesis*; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D005720:Gamma Rays; D006027:Glycosides / Q000096:biosynthesis; D009154:Mutation / Q000187:drug effects / Q000528:radiation effects; D009608:Nitrous Acid / Q000494:pharmacology; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
D000880:Anthraquinones; D000903:Antibiotics, Antineoplastic; D006027:Glycosides; D009608:Nitrous Acid