title: Peroxidase from human cervical mucus. The isolation and characterisation.
abstract: A peroxidase has been isolated from pooled specimens of human cervical mucus. Investigation of substrate and inhibitor specificity, pH optimum, chromatography on Sephadex G-200, disc electrophoresis, ultraviolet absorption spectrum and the effects of temperature and ionic strength variation are described. A study of the steady-state kinetics is reported using hydrogen peroxide and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) as substrates and a catalytic mechanism is proposed.
mesh_terms: D002582:Cervix Mucus / Q000201:enzymology*; D005260:Female; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D010544:Peroxidases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D010544:Peroxidases