title: Immunoelectron microscopic and immunochemical demonstrations of serum proteins in the alveolar lining material of the rat lung.
abstract: By means of antibodies labeled with horseradish peroxidase, autologous albumin and IgG were visualized in electron microscopy on the alveolar epithelial surface of the rat lung, as a continuous cell coat, as free granular deposits, or associated with tubular myelin figures. These 2 proteins were detected by immunochemistry in alveolar washing. After ultracentrifugation of alveolar washing, they were still found in the phospholipid-rich precipitate fraction by immunization of rabbits and by immunoelectron microscopy. Thus, albumin and IgG appeared as normal alveolar components in the rat lung; fibrinogen was not found under normal conditions.
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D006735:Horseradish Peroxidase; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000032:analysis; D008854:Microscopy, Electron; D011650:Pulmonary Alveoli / Q000032:analysis*; D011663:Pulmonary Surfactants / Q000032:analysis; D011817:Rabbits / Q000276:immunology; D051381:Rats; D012709:Serum Albumin / Q000032:analysis; D014404:Tubulin
chemical_list: D001798:Blood Proteins; D007074:Immunoglobulin G; D011663:Pulmonary Surfactants; D012709:Serum Albumin; D014404:Tubulin; D006735:Horseradish Peroxidase