Induction of acetylcholine receptors in muscle cultures.
Acetylcholine receptors in muscle cells differentiated in vitro were monitored by using 125 I-alpha-bungarotoxin. The number of cholinergic receptors was increased 4-8 fold in 2 days due to inhibition of spontaneous contraction of the muscle fibers. The inhibition of this activity, whether mediated through tetrodotoxin, lidocaine or D-600, did not affect the biochemical differentiation of muscle, as represented by creatine-phosphokinase and acetylcholinesterase activity. "Induction" of receptors by tetrodotoxin was inhibited by cycloheximide, actinomycin-D, or 5-bromotubericidine. Dystrophic muscle responded in vitro to inhibition of contraction similar to normal tissue.
D000109:Acetylcholine / Q000494:pharmacology; D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D002642:Chick Embryo; D003402:Creatine Kinase / Q000378:metabolism; D046508:Culture Techniques; D004558:Electric Stimulation; D005711:Gallopamil / Q000494:pharmacology; D008012:Lidocaine / Q000494:pharmacology; D051379:Mice; D009119:Muscle Contraction; D009124:Muscle Proteins / Q000096:biosynthesis; D009132:Muscles / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011950:Receptors, Cholinergic / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology; D014372:Tubercidin / Q000031:analogs & derivatives / Q000494:pharmacology
D002038:Bungarotoxins; D009124:Muscle Proteins; D011950:Receptors, Cholinergic; D005711:Gallopamil; D013779:Tetrodotoxin; D012313:RNA; D008012:Lidocaine; D003402:Creatine Kinase; D000110:Acetylcholinesterase; D014372:Tubercidin; D000109:Acetylcholine