Radiobiochemistry of phytodrugs: I. role of juvenile hormones and analogs in the biosynthesis of proteins and RNA in drosophila larvae.
The present communication concerns the investigations of the effect of Juvenile Hormone (J.H.) and its analogs (J.H.A.), such as Altosid (R) and Altozar(R), used as insecticides, on the biosynthesis of proteins and RNA in subcellular preparations of drosophila larvae with Leucine-U-14C and Uridine-2-14C respectively. The incorporation of leucine into proteins decreases progressively from crude homogenate to the microsome-free fraction in the same way as the degradation of the compounds. During RNA biosynthesis the incorporation of uridine increases from the crude homogenate up to mithocondria-free fraction and with Altosid is always below the test controls. The reduced incorporation of uridine into RNA and leucine into proteins in the presence of Altosid and Altozar indicates a genetic action of this compounds comparable to that of natural J.H. but slightly more efficient.
D000818:Animals; D004330:Drosophila / Q000378:metabolism*; D066298:In Vitro Techniques; D007301:Insect Hormones / Q000502:physiology*; D007447:Invertebrate Hormones / Q000502:physiology*; D007814:Larva / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D013347:Subcellular Fractions / Q000378:metabolism; D014498:Uracil / Q000378:metabolism
D007301:Insect Hormones; D007447:Invertebrate Hormones; D014498:Uracil; D012313:RNA; D007930:Leucine