Purification of malted-barley endo-beta-D-glucanases by ion-exchange chromatography: some properties of an endo-barley-beta-D-glucanase.
Two endo-beta-D-glucanases which act, respectively, on (1 leads to 3)-beta-D-glucans and barley beta-D-glucan have been isolated from malted barley, and purified by ion-exchange chromatography. The latter enzyme is highly specific for barley beta-D-glucan, and has no action on either (1 leads to 3)- or (1 leads to 4)-beta-D-glucans. It will also act on dyed barley-beta-D-glucan. Certain group-specific reagents inhibit the endo-barley-beta-D-glucanase and the endo-(1 leads to 3)-beta-D-glucanase to similar extents.
D002852:Chromatography, Ion Exchange; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D001467:Hordeum / Q000201:enzymology; D007700:Kinetics; D010944:Plants / Q000201:enzymology*; D011134:Polysaccharides
D011134:Polysaccharides; D006026:Glycoside Hydrolases