Determination of diminazene in plasma by high-performance liquid chromatography.
An analytical method is described for determining diminazene levels in plasma of cattle. Diminazene is reduced to 4-aminobenzamidine which is extracted and assayed by high-performance ion pair reverse phase partition chromatography. 3,4-Dimethoxybenzamidine served as an internal standard. Diminazene levels as low as 0.1 microgram per ml were detected.
D000578:Amidines / Q000097:blood*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004133:Diminazene / Q000097:blood*; D008722:Methods
D000578:Amidines; D004133:Diminazene