The parameters of the binding of estradiol and progesterone to their antibodies in presence of rat plasma.
The binding of estradiol and progesterone to the corresponding antibodies has been studied in system containing : a) antibody and hormone or b) antibody, hormone and rat plasma. In sytem a) the apparent association constant and the concentration of the antibody have been determined and the optimal conditions of a standard antibody-assay have been elaborated. In system b) the specific binding of hormone to antibody in presence of rat plasma has been measured, and the value of the "Operational Association Constant", Kop, has been calculated. Kop has the dimensions of an apparent association constant : it defines the ratio hormone bound/unbound to antibody, as a function of hormone concentration in system b). Based upon these experiments, the extent of hormone-binding to antibody injected to rats can be predicted. When antibody and hormone concentrations are measured in the plasma, the concentrations of hormone bound to antibody can be calculated.
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D001798:Blood Proteins; D004958:Estradiol / Q000097:blood* / Q000276:immunology; D005260:Female; D008954:Models, Biological; D011247:Pregnancy; D011374:Progesterone / Q000097:blood* / Q000276:immunology; D011485:Protein Binding; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats
D000906:Antibodies; D000936:Antigen-Antibody Complex; D001798:Blood Proteins; D011374:Progesterone; D004958:Estradiol