Morphological differences in the interactions between human mononuclear cells and coated or uncoated sheep red blood cells: a freeze-etch study of different types of rosettes.
Enriched preparations of E, EA and EAC rosettes formed by human peripheral blood mononuclear cells were freeze-etched and examined electron-microscopically. In E rosettes only lymphocyte were involved, whereas in EA and EAC rosettes lymphocytes and mononuclear phagocytes participated as rosette-forming cell. In EA and EAC rosettes, cytoplasmic extensions of the rosette forming cell were seen to penetrate the sheep red blood cell, whereas E rosettes showed a broad zone of adherence without penetration. None of the three types of rosettes showed an interspace between the membranes. Unlike E rosettes, EA and EAC rosettes showed polarity in the adherence of sheep red blood cells. These observations made by freeze-etch electron microscopy indicate distinct morphological differences between rosettes formed with coated or uncoated erythrocytes.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003176:Complement C3; D004912:Erythrocytes / Q000648:ultrastructure*; D005613:Freeze Etching; D006801:Humans; D007104:Immune Adherence Reaction; D008214:Lymphocytes / Q000276:immunology / Q000648:ultrastructure*; D009000:Monocytes / Q000648:ultrastructure*; D010586:Phagocytes / Q000648:ultrastructure; D011947:Receptors, Antigen, B-Cell; D012756:Sheep / Q000276:immunology
D003176:Complement C3; D011947:Receptors, Antigen, B-Cell