[Separation by high-performance liquid chromatography of phenylthiohydantoin derivatives of amino acids released by Edman degradation (author's transl)].
The separation by high-performance liquid chromatography of seventeen phenylthiohydantoin derivatives of amino acids released after Edman degradation of proteins is described. Sixteen phenylthiohydantoin derivatives of amino acids are differentiated within 40 min by liquid-liquid partition on a column packed with Micropak CN (moderately polar alkyl nitrile phase bonded on 10mum porous silica microparticles) and gradient elution with hexane=methylene chloride-isopropanol mixtures.
D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006827:Hydantoins / Q000032:analysis*; D010669:Phenylthiohydantoin / Q000031:analogs & derivatives / Q000032:analysis*; D011506:Proteins / Q000032:analysis; D012997:Solvents
D000596:Amino Acids; D006827:Hydantoins; D011506:Proteins; D012997:Solvents; D010669:Phenylthiohydantoin
Séparation par chromatographie en phase liquide rapide des dérivés phénylthiohydantoine des amino-acides rencontrés lors de la dégradation d'Edman