A simple method of lymphocyte purification from human peripheral blood.
We present a simple technique for obtaining cell suspension from human peripheral venous blood containing on average 96% lymphocytes, no macrophages and leukocyte red blood cell ratio 1 : 1. Cells are readily prepared under sterile conditions and can be used in culture experiments. The high yield of lymphocytes and the avoidance of Ficoll-Triosil makes the technique especially valuable for micro cytotoxicity tests such as those aimed at measuring tumour immunity.
D000328:Adult; D001799:Blood Sedimentation; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation; D005260:Female; D005337:Fibrin; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils
D005337:Fibrin