Effects of castration, testosterone, estradiol, and prolactin on specific prolactin-binding activity in ventral prostate of male rats.
Lactoperoxidase-catalyzed 125I-labeled ovine prolactin (PRL) was found to bind specifically to particulate membrane fractions of rat ventral prostate. Unlabeled PRL readily displaced the labeled PRL, whereas ovine GH, LH, FSH, or TSH showed no such competition. Castration reduced the binding of 125I-labeled PRL to about 1/6 of that in intact rats, and injections of testosterone propionate (TP) increased PRL binding to values as great or greater than those in intact controls. Injections of TP into intact immature and mature rats also increased PRL binding. In vitro binding of labeled PRL was inhibited in prostatic tissue removed from intact immature rats 2 h after injecting unlabeled PRL, but not in ventral prostates from rats killed 26 or 74 h after injecting unlabeled prolactin. PRL injected together with TP in castrated rats produced no greater increase in prolactin binding than TP alone, while estrogen appeared to decrease PRL binding beyond that produced by castration alone.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002369:Castration; D004873:Ergolines / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D013006:Growth Hormone / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D008566:Membranes / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D011388:Prolactin / Q000378:metabolism* / Q000494:pharmacology; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013737:Testis / Q000502:physiology; D013739:Testosterone / Q000494:pharmacology*; D013972:Thyrotropin / Q000494:pharmacology
D004873:Ergolines; D013739:Testosterone; D004958:Estradiol; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D013006:Growth Hormone