[Conditions for glycogen retention in smears of isolated cells. I. Cytofluorimetric analysis of glycogen content in cells obtained by different methods of isolation].
A cytofluorimetrical study was made of glycogen content on smears of isolated rat liver cells, obtained by perfusion of different solutions: 1/15 M phosphate buffer, the Locke solution, only the Locke solution plus sodium citrate, a calcium-free Locke solution, 0.25 M sucrose, physiological saline, Versene. No loss of glycogen occurred during any perfusion procedure, however, it took place during the smear preparation. The main cell injury is observed due to mechanical factors. An additional treatment of liver, after perfusion, by homogenization and centrigugation at mild conditions decreases the cell glycogen content by 30%. The least cell injury and the best glycogen retention was achieved when phosphate buffers were employed for cell isolation.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D006651:Histocytochemistry; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008112:Liver Glycogen / Q000378:metabolism*; D051381:Rats; D013050:Spectrometry, Fluorescence
D008112:Liver Glycogen
Issledovanie usloviĭ sokhraneniia glikogena na mazkakh izolirovannykh kletok. I. Tsitofluorimetricheskiĭ analiz soderzhaniia glikogena v kletkakh, poluchennykh razlichnymi metodami.