Rapid radioimmunoassay of total urinary estriol.
We describe a radioimmunoassay for total urinary estriol in pregnancy. A 25-mul aliquot of the urine specimen is acid hydrolyzed, neutralized, and diluted before assay. We use rabbit antisera against estriol-6-(O-carboxymethyl)oxime/bovine serum albumin and ammonium sulfate precipitation at room temperature. Results are unaffected by glucose or methenamine mandelate, a urinary tract antiseptic. Using semi-automatic pipetting equipment, one laboratory technologist can complete 50 assays within 8 h. This technique is both reliable and convenient and should decrease the expense of routine estriol assays.
D003124:Colorimetry / Q000379:methods; D003429:Cross Reactions; D004964:Estriol / Q000652:urine*; D005260:Female; D006801:Humans; D008709:Methenamine / Q000494:pharmacology; D008832:Microchemistry; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods; D013997:Time Factors
D004964:Estriol; D008709:Methenamine