[Characteristics and use of the "ROTOP-insulin-test'' for radioimmunologic determination of insulin].
The authors describe characteristics and clinical application of the "ROTOP-insulin-test'' set. Radioimmunological insulin determination is carried out by the principle of reverse titration: 50 ml of plasma is incubated for 18 hours with antiserum. The remaining free valencies of insulin antibodies are bound with the excess of 125I-insulin. Bound 125I-insulin is precipitated after the addition of alcohol. The variation coefficient is 7.4% in one batch and 9.1% in analysis in 48 hours (insulin concentration = 2.08 ng/ml = 49 mcU/ml). The precision index of the standard curve was 0.022. Insulin secretion was analyzed in 107 healthy persons with a normal body weight under conditions of prolonged glucose infusion. Two phases of insulin secretion were shown.
D006801:Humans; D007328:Insulin / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*
D007328:Insulin
Kharakteristika i primenenie nabora "ROTOP-insulin-test'' dlia radioimmunologicheskogo opredeleniia insulina