[Obtaining purified staphylococcal enterotoxin type B].
The authors elaborated a method of purification of staphylococcus enterotoxin, type B, consisting of three stages: the first stage--lyophilization and dialysis of the culture fluid against distilled water for 36 hours, the second stage--precipitation of dialyzed preparation with 2 volumes of ethanol at --15 degrees C, and the third stage--gel-chromatography of the enterotoxin preparation precipitated with alcohol on a column with Sephadex G-100 in a 0.015 M solution of NaCl (pH 7.0). Preparation isolated by the mentioned method possessed enterotoxic activity in a dose of 0.015 mg of protein per 1 kg of cat weight; by antigenic composition it represented a two-component system. In immunization of rabbits with a purified enterotoxin preparation an antiserum with the prevalence of antibodies to enterotoxin, type B, was obtained.
D004768:Enterotoxins / Q000302:isolation & purification*; D008722:Methods; D013211:Staphylococcus aureus / Q000032:analysis*
D004768:Enterotoxins
Poluchenie ochishchennogo stafilokokkovogo énterotoksina tipa B