Molecular forms and activities of glycosidases in cultures of amniotic-fluid cells.
Ion-exchange chromatography of gel filtration demonstrated the presence of different molecular forms of nine lysosomal enzymes in cultured amniotic-fluid cells. The patterns of molecular forms were similar to those known from skin fibroblasts and liver tissue. During cultivation total enzyme activities fluctuated with the number of passages, without any consistent trend of increase or decrease, and without correlation to the dominating cell type in the culture.
D000118:Acetylglucosaminidase / Q000032:analysis; D000653:Amniotic Fluid / Q000166:cytology / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D005696:Galactosidases / Q000032:analysis; D005959:Glucosidases / Q000032:analysis; D005966:Glucuronidase / Q000032:analysis; D006026:Glycoside Hydrolases / Q000032:analysis*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007700:Kinetics; D008361:Mannosidases / Q000032:analysis; D011247:Pregnancy; D005644:alpha-L-Fucosidase / Q000032:analysis
D007527:Isoenzymes; D005696:Galactosidases; D005959:Glucosidases; D006026:Glycoside Hydrolases; D008361:Mannosidases; D005966:Glucuronidase; D005644:alpha-L-Fucosidase; D000118:Acetylglucosaminidase