Myocardial function during hypoxia in the presence of different buffers.
Electrically driven rabbit left atria were exposed to 20 min periods of either hypoxia or anoxia in the presence of a bicarbonate-phosphate buffer, a Tris buffer [tris (hydroxymethyl) aminomethane hydrochloride] or combination of both. The bicarbonate-phosphate buffer system was shown to be important for tissue survival during hypoxia or anoxia whereas recovery was diminished in the presence of Tris only. Stimulus threshold and arrhythmias were shown to increase for atria in Tris. Oxygen consumption determinations on both spontaneously beating right atria and quiescent left atria showed no difference between pre- and post-hypoxia or between different buffers. Tris was shown to elicit a positive inotropic effect without an increase in O2 consumption.
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000139:chemically induced; D001639:Bicarbonates / Q000494:pharmacology; D002021:Buffers; D005260:Female; D005947:Glucose / Q000378:metabolism; D006321:Heart / Q000503:physiopathology*; D006325:Heart Atria / Q000503:physiopathology; D000860:Hypoxia / Q000378:metabolism / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D010710:Phosphates / Q000494:pharmacology; D011817:Rabbits; D012996:Solutions; D013997:Time Factors; D014325:Tromethamine / Q000494:pharmacology
D001639:Bicarbonates; D002021:Buffers; D010710:Phosphates; D012996:Solutions; D014325:Tromethamine; D005947:Glucose; D010100:Oxygen