Radioimmunoassay for normalized thyroxine with use of Sephadex columns.
We describe a reproducible radioimmunoassay, with use of Sephadex columns, for measuring normalized thyroxine. In comparison with a competitive protein-binding procedure, the present method is more specific because antibody rather than thyroxine-binding globulin is responsible for the competition between endogeneous and tracer thyroxine. In barbital buffer, various concentrations of thyroxine binding pre-albumin and albumin had no influence on the results. Values obtained by the present method correlated well with those by the free thyroxine index and a generally accepted thyroxine normalized method.
D001798:Blood Proteins; D006801:Humans; D011228:Prealbumin; D011863:Radioimmunoassay / Q000379:methods; D012685:Sepharose; D013974:Thyroxine / Q000097:blood*; D014284:Triiodothyronine / Q000097:blood
D001798:Blood Proteins; D011228:Prealbumin; D014284:Triiodothyronine; D012685:Sepharose; D013974:Thyroxine