[Studies on the cell wall of yeast of the genus Rhodotorula. X. Isolation and purification of cell-wall glycoproteins from Rhodotorula rubra (author's transl)].
Following ethylene diamine treatment of the cell wall of Rhodotorula rubra, a water soluble fraction has been isolated. This fraction can be resolved into three glycoproteins and one protein. The major part is a glycoprotein, purified to homogeneity which has a molecular weight of 64 000. The glyco-part of this protein contains mannose, glucose and one residue of glucosamine. After pronase treatment, the presence of an ""Asparaginyl-N-acetylglucosamine" linkage is established by the existence of one aspartic acid residue and one glucosamine residue. After permethylation, the initial data give some evidence that the branching points in the molecule were the carbon atoms 3 and (or) 6 of the mannose moiety and that some glucose moieties are bound to the non-reducing terminal end.
D000117:Acetylglucosamine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D001224:Aspartic Acid / Q000032:analysis; D002473:Cell Wall / Q000032:analysis*; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006023:Glycoproteins / Q000302:isolation & purification; D006601:Hexoses / Q000032:analysis; D008358:Mannose / Q000032:analysis; D003904:Mitosporic Fungi / Q000032:analysis*; D012248:Rhodotorula / Q000032:analysis*
D000596:Amino Acids; D006023:Glycoproteins; D006601:Hexoses; D001224:Aspartic Acid; D005947:Glucose; D005944:Glucosamine; D008358:Mannose; D000117:Acetylglucosamine
Etudes des parois de levures du genre Rhodotorula. X. Isolement et purification de glycoprotéines de la paroi de Rhodotorula rubra