[Enzyme diagnosis in lipaemic sera before and after polyanion precipitation with heparin and magnesium chloride (author's transl)].
The method for the determination of enzymic activity in turbid, lipaemic sera, which involves clearing by polyanion precipitation with heparin and magnesium chloride, was critically reviewed. In the diagnosis of diseases of the liver and pancreas, which are frequently associated with hyperlipoproteinaemia, only residual enzyme activities are measured in the cleared serum after polyanion treatment. In the measurement of glutamate dehydrogenase and in the Phadebas test for alpha-amylase, the enzymes are inactivated by treatment with heparin and magnesium chloride. On the other hand, as a result of polyanion precipitation gamma-glutamyl transferase is transferred, together with lipoproteins and chylomicrons, to the lipid-rich supernatant. Acid phosphatase also exhibits only residual activity in cleared serum. The activity of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, leucine arylamidase, cholinesterase, creatine kinase, lactate dehydrogenase, and alpha-hydroxybutyrate dehydrogenase, and the activity of alpha-amylase in the Merckotest are not affected by polyanion treatment of the serum.
D000838:Anions; D011232:Chemical Precipitation; D004796:Clinical Enzyme Tests; D004791:Enzyme Inhibitors; D006493:Heparin; D006801:Humans; D008055:Lipids / Q000097:blood*; D008107:Liver Diseases / Q000175:diagnosis / Q000201:enzymology; D008274:Magnesium; D010182:Pancreatic Diseases / Q000175:diagnosis / Q000201:enzymology
D000838:Anions; D004791:Enzyme Inhibitors; D008055:Lipids; D006493:Heparin; D008274:Magnesium
Enzymdiagnostik in lipämischen Seren vor und nach Polyanionenpräzipitation mit Heparin und Magnesiumchlorid.