Purification and biochemical properties of Egtved viral RNA.
32P-labeled Egtved virus RNA was released from highly purified virus by phenol-SDS extraction. The single-stranded nature of the RNA was demonstrated by (1) its buoyant density of 1.69 g/cm3 in Cs2-SO4, (2) its susceptibility to digestion by pancreatic ribonuclease in either 1 X SSC or 0.01 X SSC (standard saline citrate), (3) its base composition (29.3% C, 23.6% A, 14.5% U. 32.6% G). This Egtved virus is different from the other rhabdoviruses since the base composition of its genomic RNA is lower in its composition of A + U. Such a result could have possible taxonomic implications concerning the orignin and evolution of Egtved Virus relative to the other known rhabdoviruses.
D000225:Adenine / Q000032:analysis; D002499:Centrifugation, Density Gradient; D003596:Cytosine / Q000032:analysis; D006147:Guanine / Q000032:analysis; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000032:analysis*; D012209:Rhabdoviridae / Q000032:analysis*; D012260:Ribonucleases; D014498:Uracil / Q000032:analysis
D012367:RNA, Viral; D014498:Uracil; D006147:Guanine; D003596:Cytosine; D012260:Ribonucleases; D000225:Adenine