title: [Simplified techniques for toxoplasmosis studies (author's transl)].
abstract: Some improvements on laboratory technics for Toxoplasmosis are presented: 1--the use of serum from guinea-pigs as accessory factor (AF) on the dye-test; 2--the use of filter-paper discs for collecting and storing blood of children and small animals for the dye test; 3--conservation of Toxoplasma gondii at various media and temperatures.
  
  
  RESULTS
  1--the pool of serum from 14 guinea-pigs gave good results when diluted in 1/5 Alsever solution at the proportion of 6/10; 2--the blood from digital puncture usually could be collected on filter-paper discs of 15 mm of diameter and stored at the freezer to -23 degrees C for 3-4 weeks, protected with alumen-paper. The dilution with saline 8.5% could be made 1/2 hour before starting the procedure; 3--the Toxoplasma gondii (cysts and trophozoites) could be stored at LIT medium or saline 8.5% at refrigator a +4 degrees C for 15-22 days, with some reduction in virulence.
mesh_terms: D000818:Animals; D005374:Filtration / Q000295:instrumentation; D006168:Guinea Pigs; D006801:Humans; D008751:Methylene Blue; D012698:Serologic Tests / Q000379:methods; D014123:Toxoplasmosis / Q000175:diagnosis*
chemical_list: D008751:Methylene Blue
vernacular_title: Simplificacões em técnicas para o estudo da toxoplasmose