[The compact form of DNA in solution. V. The heat effect preceding compactization of double-chained DNA in PEG containing water-salt solutions].
DNA-dependent heat effects accompanying mixing of water-salt (0.3 M NaCl) solutions of PEG and DNA within the range of PEG 10-50 mg/ml at 25 degrees C were determined by the method of difference microcalorimetry. It was found that, unlike optical and hydrodynamical methods, microcalorimetry makes it possible to detect some changes of the DNA-PEG system preceding formation of compact particles of DNA. In the studied range of DNA concentrations (up to 50 X 10(-3) MG/ML) the specific DNA-dependent heat effect is essentially independent of DNA concentration. It is negative and its absolute value increases from 0 to 5 cal/g of DNA in the PEG concentration range from 0 to 35-40 mg/ml after that the rate of its increase raises greatly and at PEG concentration of 50 mg/ml it is equal to 35 cal/g of DNA. It is suggested that the studied DNA dependent heat effects at low concentrations of PEG (less than 40 mg/ml) are caused by dehydration of DNA preceding its compactization.
D004247:DNA; D006358:Hot Temperature; D009690:Nucleic Acid Conformation; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D012996:Solutions
D012996:Solutions; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D004247:DNA
Kompaktnaia forma DNK v rastvore. V. Teplovoĭ éffekt, predshestvuiushchiĭ kompaktizatsii dvukhtsepochechnoĭ DNK v soderzhashchikh PEG vodno-solevykh rastvorakh