Dispersion of fibrin using cationic detergent.
By treating fibrin with cationic detergent in a medium containing 5M urea, a fibrincationic detergent complex was formed. The complex was soluble in distilled water, and was observed to be in a dispersed state by electron microscopy. The complex was precipitated and resolubilized in the presence of salt. This behavior of the complex was due to adsorption of the salt anions onto the complex. When an aqueous solution of the complex was incubated with fresh serum, the complex soon aggregated and then was converted into a firm clot which was not soluble in distilled water.
D001769:Blood; D011232:Chemical Precipitation; D003840:Deoxycholic Acid; D003902:Detergents; D005292:Ferricyanides; D005337:Fibrin; D006801:Humans; D011485:Protein Binding; D000644:Quaternary Ammonium Compounds; D014508:Urea
D003902:Detergents; D005292:Ferricyanides; D000644:Quaternary Ammonium Compounds; D003840:Deoxycholic Acid; D014508:Urea; D005337:Fibrin