The effect of nitrosoguanidine upon DNA synthesis in vitro.
Both the polymerase and the exonuclease activities of DNA polymerase III are inactivated by treatment with nitrosoguanidine. The treatment of the DNA template with the mutagen does not affect the template in supporting DNA synthesis. No effect of nitrosoguanidine upon fidelity of replication in vitro was detected.
D002273:Carcinogens; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D005092:Exonucleases / Q000378:metabolism; D066298:In Vitro Techniques; D009153:Mutagens; D009604:Nitrosoguanidines / Q000494:pharmacology*; D013698:Templates, Genetic
D002273:Carcinogens; D009153:Mutagens; D009604:Nitrosoguanidines; D004254:DNA Nucleotidyltransferases; D005092:Exonucleases