The effect of short-term labelling in (3H) thymidine on the viability of mouse blastomeres: alone and in combination with unlabelled blastomeres.
A method of labelling 8-cell-stage mouse blastomeres with [3H]thymidine is described, which allows them to be followed to the late blastocyst stage and is compatible with normal postimplantation development. However, the [3H]thymidine does affect the postimplantation vigour of the cells when placed in competition with unlabelled cells in blastomere aggregates. This suggests that caution should be used in interpreting results using [3H]thymidine as a label for early mammalian cells.
D000818:Animals; D001755:Blastocyst / Q000528:radiation effects*; D002452:Cell Count; D002455:Cell Division / Q000528:radiation effects; D002678:Chimera; D010064:Embryo Implantation / Q000528:radiation effects; D004622:Embryo, Mammalian / Q000201:enzymology; D005260:Female; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D051379:Mice; D014316:Tritium
D014316:Tritium; D005956:Glucose-6-Phosphate Isomerase