A sensitive assay for proteases using a clot timer apparatus.
This report describes the adaptation of milk coagulation by proteases into a quantitative and sensitive assay for these enzymes. The procedure involves the use of a clot timer to determine the precise phase of the coagulation which occurs when buffered milk and a protease interact.
D000818:Animals; D001665:Binding Sites; D002846:Chromatography, Affinity; D002918:Chymotrypsin / Q000032:analysis* / Q000302:isolation & purification; D046911:Macromolecular Substances; D008832:Microchemistry; D008892:Milk; D011485:Protein Binding; D013997:Time Factors
D046911:Macromolecular Substances; D002918:Chymotrypsin