Formation of hybrid concanavalin A molecules by subunit exchange.
Mixing of native concanavalin A (Con A) and its dimeric succinylated derivative (succinyl-Con A) in glycine-HCl buffer, pH 4.5, resulted in the formation of a new chemical species that could be separated as a unique fraction by DEAE-cellulose chromatography or by gel electrophoresis. The molecular weight of this new component, which contained the subunits (Mr= 26,000) of native Con A and its succinyl derivative in equimolar amounts, was 50,000 at both pH 5 and pH 7. These data suggest that subunit exchange between 2 chemically distinct Con A molecules yields a hybrid molecule consisting of one protomer of native Con A and one protomer of succinyl-Con A. Similar exchange reactions and hybrid molecules were also observed after mixing acetyl-Con A and succinyl-Con A. These procedures provide several new chemical variants of the Con A molecule that may be useful for the analysis of lectin-cell surface interactions.
D001665:Binding Sites; D003208:Concanavalin A; D046911:Macromolecular Substances; D011485:Protein Binding; D011487:Protein Conformation; D013386:Succinates
D046911:Macromolecular Substances; D013386:Succinates; D003208:Concanavalin A