Molecular-size-dependent degradation of liver cytosolic proteins in vitro.
Degradation of rat liver cytosolic proteins at a neutral pH in the presence of 0.1% SDS was demonstrated by SDS-acrylamide gel electrophoresis. This proteolysis in vitro mimics molecular-size-dependent proteolysis in vivo; larger proteins were degraded more rapidly than smaller ones. Evidence is presented that the proteolysis is not due to contaminating lysosomal cathepsins in the cytosol.
D000818:Animals; D003600:Cytosol / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008970:Molecular Weight; D011506:Proteins / Q000378:metabolism*; D051381:Rats
D011506:Proteins