Effects of estradiol on in vitro nucleoside incorporation and methylation of rat uterine ribonucleic acid.
The in vitro nucleoside incorporation and methylation of uterine RNA was examined by incubating immature rat uteri with either 3H-uridine, 3H-uridine-3H-cytidine, and/or 14C-methyl-methionine. Radioactivity was determined in extracted RNA which was fractionated by gel electrophoresis or by differential solubility in zine acetate or lithium chloride. Estradiol (E2)-treated uteri respond by showing a maximum in vitro incorporation of nucleosides into total RNA and high M.W. RNA within one hr of in vivo treatment. Nucleoside incorporation into low M.W. RNA increased gradually throughout the first 3 hrs of a 4-hr hormone treatment period. E2 in vivo also caused increased in vitro methylation of total, high, and low M.W. uterine RNA fractions. Methylation of all fractions increased through the first 3 hrs of hormone treatment, but greater methylation of low M.W. RNA was apparent. The data indicate that in vivo E2 treatment stimulates an initial transitory stage of in vitro nucleoside incorporation into uterine RNA followed by a more prolonged interval of RNA methylation.
D000818:Animals; D003562:Cytidine / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008715:Methionine / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D014529:Uridine / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
D004958:Estradiol; D003562:Cytidine; D012313:RNA; D008715:Methionine; D014529:Uridine