[Effect of phenylalanine and alpha-tocopherol on ubiquinone and ubichromenol metabolism in the liver of E-avitaminotic rats in vitro].
When incubating the liver pulp of vitamin E-deficiency rats with 2-14C-sodium acetate and phenylalanine for an hour the content and radioactivity of ubiquinone and ubichromenol increase as compared to the control level. In this respect phenylalanine in in a dose of 60 and 120 mumol per 1 g of the liver pulp is most effective. Under conditions of these experiments incubation for 3 h causes no noticable changes in the content and metabolism of ubiquinone and ubichromenol in the liver pulp of vitamin E-deficiency rats as compared to the control level. Introduction of different amounts of alpha-tocopherol into the incubation medium results 3 h after an increase in concentration and radioactivity of ubiquinone, the alpha-tocopherol dose of 116 nmol being the most effective. An increase in the concentration and radioactivity of ubichromenol occurs at lower doses of exogenous alpha-tocopherol (58 nmol). All the used doses of alpha-tocopherol cause a decrease in intensity of radio-label incorporation into the unsaponifiable fraction of vitamin E-deficiency rat liver incubated for 3 h. Phenylalanine also results in a decrease of these values with the system incubation for an hour and produces no changes in them in the 3 h incubation. The results of the experiments give grounds to suppose the presence of interrelation between alpha-tocopherol and biosynthesis of ubiquinone and ubichromenol in the rat liver.
D000818:Animals; D001578:Benzopyrans / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002839:Chromans / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D010649:Phenylalanine / Q000494:pharmacology*; D051381:Rats; D012588:Scintillation Counting; D013997:Time Factors; D014451:Ubiquinone / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*; D014811:Vitamin E Deficiency / Q000378:metabolism*
D001578:Benzopyrans; D002250:Carbon Radioisotopes; D002839:Chromans; D014451:Ubiquinone; D014810:Vitamin E; C003740:ubichromenol; D010649:Phenylalanine
Vplyv fenilalaninu ĭ al'fa-tokoferolu na obmin ubikhinonu ta ubikhromenolu v pechintsi E-avitaminoznykh shchuriv in vitro