Heterogeneity of extractable gut glucagon-like immunoreactivity (GLI) and its lipolytic activity.
Gut glucagon-like immunoreactivity (GLI) was extracted from the mucosa of the canine intestine and was separated into two peaks by gel filtration; Peak I (7,000 daltons) and Peak II (3,500 daltons). These two peaks were purified by means of affinity chromatography using an anti-glucagon antibody as a ligand substance. These purified gut GLIs were not composed of single component, when applied to ion exchange chromatography. These partially purified Peak I and Peak II showed lipolytic activities, though very weak in potency, in vitro on the rat fat tissue at pharmacologically high concentration (5 ng eq/ml in final). It was concluded that gut GLI, even though purified, showed heterogeneity and that gut GLI revealed lipolytic activity although weak in potency compared with pancreatic glucagon.
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D000941:Antigens; D004285:Dogs; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005934:Glucagon / Q000276:immunology* / Q000302:isolation & purification; D005990:Glycerol / Q000378:metabolism; D007413:Intestinal Mucosa; D007421:Intestine, Small; D008053:Lipid Mobilization / Q000187:drug effects; D008297:Male; D051381:Rats
D000941:Antigens; D005230:Fatty Acids, Nonesterified; D005934:Glucagon; D005990:Glycerol