[A study of thyroglobulin biosynthesis by the incorporation of glucosamine-14C, mannose-14C and galactose-14C in thyroid gland cell-free systems].
The authors studied in the microsomal and polyribosomal protein-synthesizing system of the thyroid gland a combination of carbohydrate components of thyroglobulin by the incorporation of labeled D-glucosamine-14C, D-mannose-14C, and galactose-14C. It was shown that in the process of protein biosynthesis there occurred in the microsomal system a combination of carbohydrate-14C; no combination of carbohydrates was noted in the polyribosomic system. Analysis of the synthesized proteins was carried out by disc-electrophoresis. All the carbohydrates under study were revealed both in the 19S molecule of thyroglobulin and in its 3-8 subunits. Thus, a combination of carbohydrate components occurred after the termination of peptide chain synthesis and already at the microsome level.
D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D005690:Galactose / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis; D008358:Mannose / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D013954:Thyroglobulin / Q000096:biosynthesis*
D006023:Glycoproteins; D013954:Thyroglobulin; D005944:Glucosamine; D008358:Mannose; D005690:Galactose
Issledovanie biosinteza tireoglobulina po vkliucheniiu gliukozamina-14C, mannozy-14C i galaktozy-14C v beskletochnoĭ sisteme schitovidnoĭ zhelezy