The human leukocyte test system. VIII. DNA synthesis and mitoses in 3-day cultures stimulated with pokeweed mitogen.
In human leukocyte cultures set up with Ham's F-10 medium and stimulated with pokeweed mitogen (PWM), DNA synthesis and mitotic indices were analyzed by means of (3H)TdR autoradiography and cell counting. The results show a very similar pattern of DNA synthesis and mitotic indices to that found in cultures set up with TC medium 199 and stimulated with phytohemagglutinin (Obe et al., 1975 a).
D002869:Chromosome Aberrations; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008940:Mitotic Index
D003470:Culture Media; D037102:Lectins; D004247:DNA