[Determination of lecithin: cholesterol acyltransferase activity by a radioisotopic assay (author's transl)].
A method for determining Lecithin: Cholesterol Acyltransferase (LCAT) activity is presented. Trace amounts of labelled cholesterol are added to innactivated homologous substrate from a pool of normal serum. LCAT activity is determined measuring the cholesterol esterification, and is expressed as micrograms of cholesterol esters formed per milliliter plasma per hour. LCAT time-course, reproducibility, activity after serum storage at 4 degrees C, and normal values from 20 healthy subjects are studied.
D000217:Acyltransferases / Q000097:blood*; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000097:blood; D004951:Esterification; D006801:Humans; D008722:Methods; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood*
D002250:Carbon Radioisotopes; D002784:Cholesterol; D000217:Acyltransferases; D007862:Phosphatidylcholine-Sterol O-Acyltransferase
Determinación de la actividad lecitín: colesterol aciltransferasa por un método radioisotópico