Regulation by calcium of the proliferation of heart cells from young adult rats.
Cells of primary and secondary cultures of rat heart ventricle were grown in a medium supplemented with homologous plasma to avoid the non-specific proliferogenic effects of foreign sera. Specific chelation of calcium from the medium arrested the cells' progression through their cycle at the G1 phase. A permanent or brief restoration of the extracellular calcium level (48 hr later) was followed, after a 5 to 6 hr delay, by a burst of DNA synthesis, and the resumption of mitotic activity.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D008297:Male; D008938:Mitosis / Q000187:drug effects; D009206:Myocardium / Q000166:cytology*; D010949:Plasma; D051381:Rats
D003470:Culture Media; D004247:DNA; D002118:Calcium