Chromosome analyses in cell cultures of the Chinese hamster after application of cadmiumsulphate.
Cell cultures of a Chinese hamster cell line were treated with CdSO4 in concentrations of between 10(-4) and 10(-8) mol/l for different time periods. After a treatment of 16 h the mitotic index was reduced. Strong stickiness and pycnosis of chromosomes occurred at the highest concentrations. A treatment period of 3 h with concentrations of 10(-5) and 10(-4) mol/l without additional application of Colcemid and hypotonic solution yielded a stathmokinetic effect. The mitotic index increased, mitoses were blocked in metaphase stage and "initial C-mitoses'' and "C-mitoses'' were present. Structural chromosome aberrations were present in the recovery period of more than 12 h following a treatment of 1 h with 10(-4) mol/l CdSO4. The observed aberrations were mainly of the chromatid type.
D002104:Cadmium / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D008938:Mitosis / Q000187:drug effects*; D011123:Polyploidy; D013431:Sulfates / Q000494:pharmacology*; D013997:Time Factors
D013431:Sulfates; D002104:Cadmium