Comparison between human 125I-labelled TSH labelled with lactoperoxidase or chloramine-T; advantages in the use of enzymically labelled antigen in a radioimmunoassay for human TSH.
1. Human thyroid stimulating hormone (human TSH) was iodinated by both chloramine-T and lactoperoxidase methods. 2. Human 125I-labelled TSH prepared by the enzymic method showed less damage on storage, an increased shelf-life, and improved binding at different antiserum dilutions. 3. A comparison of the values of the bound counts on the addition of human TSH, expressed as a percentage of the zero standard bound counts (B/BO values) in the standard curves, demonstrated that lactoperoxidase-labelled human TSH gave increased sensitivity.
D002700:Chloramines; D005069:Evaluation Studies as Topic; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D007784:Lactoperoxidase; D010544:Peroxidases; D011863:Radioimmunoassay / Q000379:methods; D013972:Thyrotropin / Q000097:blood*
D002700:Chloramines; D007457:Iodine Radioisotopes; D013972:Thyrotropin; D007784:Lactoperoxidase; D010544:Peroxidases