Elimination of double strand nuclease activity from S1 nuclease prepared from crude alpha amylase.
Single strand-specific s1 nuclease prepared as previously described from crude alpha amylase by DEAE-cellulose chromatography also contains nuclease which degrades double strand nucleic acid. The double strand activity can be removed by repeating the DEAE-cellulose chromatography procedure at least two additional times. S1 nuclease prepared by this procedure does not degrade double strand sheared DNA as measured by Sephadex chromatography. Under the same conditions single strand DNA is completely degraded. Thus, S1 nuclease prepared by this procedure is suitable for use in removing single strand regions in DNA/DNA duplexes and DNA/RNA hybrids.
D000681:Amylases / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D003851:Deoxyribonucleases / Q000302:isolation & purification*; D007739:L Cells; D009994:Osmolar Concentration
D003851:Deoxyribonucleases; D000681:Amylases