[Isolation of the fragment D dimer from stabilized fibrin and a study of its antipolymerization action].
The dimer of fragment D is isolated preparatively by CM-cellulose chromatography (in neutral medium) from dialyzed tryptic hydrolyzate of stabilized fibrin. Contrary to the fragment D monomer obtained from fibrinogen under the same conditions, the dimer itself cannot inhibit polymerization of monomeric fibrin. However with the presence of monomer D in the amount sufficient for essential inhibition of the fibrin polymerization the addition of dimer D produces the further delay in polymerization. Under these conditions dimer D is highly effective inhibitor if its amount is not too little, as its small doses are not effective.
D000818:Animals; D002417:Cattle; D002845:Chromatography; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005337:Fibrin / Q000032:analysis* / Q000037:antagonists & inhibitors; D005338:Fibrin Fibrinogen Degradation Products / Q000302:isolation & purification* / Q000494:pharmacology; D005340:Fibrinogen / Q000032:analysis; D006868:Hydrolysis; D011108:Polymers; D014357:Trypsin / Q000494:pharmacology
D005338:Fibrin Fibrinogen Degradation Products; D011108:Polymers; D005337:Fibrin; D005340:Fibrinogen; D014357:Trypsin
Vydilennia dymeru frahmenta D zi stabilizovanoho fibrynu ta doslidzhennia ĭoho antypolimeryzatsiĭnoï diï