Simple, direct determination of serum 5'-nucleotidase.
A simple determination of 5'-Nucleotidase in blood serum without deproteinization is described. The enzyme activity is distinguished from that of a nonspecific alkaline phosphatase by nickel inhibition and the inorganic phosphate released from adenosine monophosphate used as substrate is determined by a method previously described. Additional studies include the determination of optimal conditions for the reaction and nickel inhibition.
D000469:Alkaline Phosphatase / Q000097:blood; D006801:Humans; D008722:Methods; D009532:Nickel / Q000494:pharmacology; D009708:Nucleotidases / Q000097:blood*
D009532:Nickel; D009708:Nucleotidases; D000469:Alkaline Phosphatase