Radiometric determination of the concentration of amphotericin B in body fluids.
A new assay was developed to determine the concentration of amphotericin B in body fluids. The Bactec radiometric system was used to measure CO(2) production by a test strain, Candida albicans MDA 448, in the presence of amphotericin B. After 5-h incubation, drug concentrations as low as 0.2 mug/ml could be detected. The results are comparable to those of the commonly used agar diffusion assay with Paecilomyces varioti.
D000666:Amphotericin B / Q000032:analysis* / Q000097:blood; D001826:Body Fluids / Q000032:analysis*; D006801:Humans; D011874:Radiometry / Q000379:methods*
D000666:Amphotericin B