Macroscopic cloning assay using complement fixation to isolate secretion variants of myeloma cells.
Mouse myeloma cells were cloned in agar and overlaid in antiserum to a mouse immunoglobulin. The resulting local immune precipitates were used as complement fixing layers to protect macroscopically visible zones of sheep erythrocytes from lysis. The immunological specificity of the assay was confirmed in mixing experiments using two different myeloma cell lines.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D003168:Complement Fixation Tests; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology*
D007070:Immunoglobulin A; D007074:Immunoglobulin G