Isolation of (copper, zinc-) thioneins from pig liver.
The copper-binding proteins with mol. wt. of approx. 12000 in the cytosol of pig liver have been identified as copper-thioneins. They contain variable amounts of zinc, with approx. 10% total metal in the protein. The molar cysteine/metal ratio is approx. 2:1.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D003300:Copper / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis*; D008667:Metalloproteins / Q000302:isolation & purification*; D008668:Metallothionein / Q000302:isolation & purification*; D011485:Protein Binding; D011506:Proteins / Q000032:analysis; D013552:Swine; D015032:Zinc / Q000032:analysis
D000596:Amino Acids; D008667:Metalloproteins; D011506:Proteins; D003300:Copper; D008668:Metallothionein; D015032:Zinc