Specific binding sites for L-triiodothyronine in rat liver and kidney cytosol.
Binding of 125I-labelled triiodothyronine (T3) to rat liver and kidney cytosol was examined. In both organs a very similar binding protein was detected. The apparent equilibrium association constants amounted to 4.8 X 10(7) M-1 for the liver protein and to 1.8 X 10(7) M-1 for the kidney protein. In the two organs a high capacity for T3 binding was observed: maximal binding capacities of respectively 4.3 and 33.2 pmol per mg total cytosol protein. Displacement studies using thyroid hormone analogues showed that the binding exhibited similar specificity.
D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins; D003600:Cytosol / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D014284:Triiodothyronine / Q000378:metabolism*
D002352:Carrier Proteins; D014284:Triiodothyronine