title: Polarographic determination of robenidine residues in chicken tissues, eggs, litter, soil, and plant tissue.
abstract: Polarographic residue methods have been developed for determining robenidine (Robenz), 1,3-bis[p-chlorobenzylidene)amino]-guanidine monohydrochloride, in chicken tissues, eggs, litter, soil, and plants. The compound is extracted from chicken fat, skin, muscle, liver, and eggs with ethyl acetate; from blood with acetone; from plant tissue, litter, and kidney with acidic acetone; and from soil with basic methanol. After extraction by high-speed blending or overnight shaking, the extract is cleaned up by evaporation, solvent partition and/or elution from CG-50 ion exchange resin. Robenidine is quantitated by differential cathode ray polarography, using acidic aqueous methanol or acetic acid (1+1) supporting electrolyte. Recoveries ranged from 64 to 125% with an average overall recovery of 90%. The validated sensitivity is 0.1 ppm for chicken tissues, soil, and plants, 0.01 ppm for eggs, and 1 ppm for litter.
mesh_terms: D000818:Animals; D002645:Chickens; D004531:Eggs / Q000032:analysis; D005243:Feces / Q000032:analysis; D006146:Guanidines / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D010944:Plants / Q000032:analysis; D011048:Polarography; D012370:Robenidine / Q000032:analysis*; D012987:Soil / Q000032:analysis; D014018:Tissue Distribution
chemical_list: D006146:Guanidines; D010573:Pesticide Residues; D012987:Soil; D012370:Robenidine