title: Reversed-phase ion-pair partition chromatography of phenylacetic, mandelic and pyridinecarboxylic acid derivatives.
abstract: Phenylacetic, mandelic and pyridinecarboxylic acids of physiological interest have been separated by ion-pair partition chromatography with 1-pentanol as stationary phase and tetrabutylammonium as counter ion in the aqueous mobile phase. The stability of the chromatographic system is very high and the support is spontaneously re-coated with stationary phase. Untreated plasma samples can be injected in relatively large volumes without serious loss of separating efficiency. A venting system was found efficiently to reduce disturbing early peaks on injection of biological samples.
mesh_terms: D002021:Buffers; D002395:Catecholamines / Q000032:analysis; D002852:Chromatography, Ion Exchange; D006801:Humans; D008333:Mandelic Acids / Q000032:analysis* / Q000097:blood; D009539:Nicotinic Acids / Q000032:analysis* / Q000097:blood; D010648:Phenylacetates / Q000032:analysis* / Q000097:blood; D012997:Solvents
chemical_list: D002021:Buffers; D002395:Catecholamines; D008333:Mandelic Acids; D009539:Nicotinic Acids; D010648:Phenylacetates; D012997:Solvents