Composition of the neutral lipids of bovine meilbomian secretions.
Lipids secreted form the bovine meibomian glands were assigned to following classes: cholesteryl esters (A) (fatty acyl chain lengths from 15 to 27 carbon atoms), 41%; wax esters, 29%; triacylglycerols, 10%; and cholesteryl ester (B) (fatty acyl chain lengths from 14 to 18 carbons), 15%. The remaining 5% consisted of cholesterol, fatty acids, and highly polar material. Analysis of the lipids showed that only the cholesteryl esters (A) contained fatty acyl chains greater than or equal to C20:0. One third of the fatty acyl chains of the wax esters and the cholesteryl esters (B) was iC15:0. The fatty alcohol moieties of the wax esters were found to be branched chain, C23:0-C27:0. A computer-based programmable gas-liquid chromatographic procedure using 10% apolar 10C on Gas Chrom Q could be used to distinguish both iso and anteiso fatty alcohols and esters of fatty acids.
D000818:Animals; D002417:Cattle; D002788:Cholesterol Esters / Q000032:analysis; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005143:Eyelids / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D005233:Fatty Alcohols / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008537:Meibomian Glands / Q000032:analysis*; D014280:Triglycerides / Q000032:analysis; D014885:Waxes / Q000032:analysis
D002788:Cholesterol Esters; D005227:Fatty Acids; D005233:Fatty Alcohols; D008055:Lipids; D014280:Triglycerides; D014885:Waxes