Characterization of the fragments obtained by enzymic and alkaline degradation of rice-bran proteoglycans.
Repeated digestion of the proteoglycan B from rice bran with Pronase and hemicellulase yielded two types of glycopeptides. The first group, obtained from the third enzymic digest, was a type of low molecular-weight glycopeptide and was fractionated into two subfractions by paper electrophoresis. The second group, obtained from the fourth enzymic digest, was a mixture of glycopeptides of relatively high molecular-weight and was fractionated into three subfractions by gel filtration on Sephadex G-75. Alkaline degradation of these glycopeptides or the intact proteoglycan A yielded a sugar-amino acid compound, whose structure was established as O-alpha-L-arabinofuranosyl-hydroxyproline. These results indicate that the carbohydrate-protein linkage of the rice-bran proteoglycan is an O-glycosyl linkage between L-arabinose and hydroxyproline.
D000596:Amino Acids / Q000032:analysis; D001089:Arabinose / Q000032:analysis; D002480:Cellulase / Q000378:metabolism; D005690:Galactose / Q000032:analysis; D006020:Glycopeptides / Q000032:analysis; D007700:Kinetics; D008970:Molecular Weight; D012275:Oryza; D011402:Pronase / Q000378:metabolism; D011509:Proteoglycans / Q000302:isolation & purification; D012639:Seeds / Q000032:analysis*; D014994:Xylose / Q000032:analysis
D000596:Amino Acids; D006020:Glycopeptides; D011509:Proteoglycans; D014994:Xylose; D001089:Arabinose; D002480:Cellulase; D011402:Pronase; D005690:Galactose