Polymorphonuclear leukocytes produce thromboxane A2-like activity during phagocytosis.
Homogenates of phagocytosing polymorphonuclear leukocytes obtained from rabbit peritoneum were incubated with the prostaglandin endoperoxides PGG2 or PGH2. After 2 min at 0degree C, incubation mixtures contained an increased rabbit aorta contracting activity. Ether extracts of incubation mixtures contained a substance which contracted the superfused strips of rabbit aorta and coeliac artery and had a half life which was similar to thromboxane A2. The generation of thromboxane A2-like activity from PG endoperoxides was prevented by boiling the homogenate prior to incubation, or by pretreatment with benzydamine, a drug which blocks thromboxane formation in platelets. Production of thromboxane A2-like material by leukocyte homogenates was compared with platelet microsomal thromboxane synthetase.
D000818:Animals; D001011:Aorta / Q000187:drug effects; D001792:Blood Platelets / Q000378:metabolism; D006736:Horses; D006358:Hot Temperature; D007962:Leukocytes / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects; D010545:Peroxides / Q000378:metabolism / Q000494:pharmacology; D010587:Phagocytosis; D011453:Prostaglandins / Q000378:metabolism* / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D011817:Rabbits
D010545:Peroxides; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F