Identification of glucuronide metabolites of d- and 1-methadone in bile from the isolated perfused rat liver.
Three hydroxylated metabolites of methadone, 2-ethyl-3-(dihydroxyphenyl)-5-methyl-3-phenylpyrroline (di-HO-EMDP), 2-ethyl-3-(hydroxy-methoxy-phenyl)-5-methyl-3-phenylpyrroline (HO-MeO-EMDP) and 2-ethyl-3-(hydroxy-phenyl)-5-methyl-3-phenylpyrroline (HO-EMDP) were excreted as O-glucuronide conjugates in bile from the isolated perfused rat liver following the addition of either d- or 1-methadone to the perfusate. These glucuronide metabolites were identified as intact molecules by combined gas chromatographic-mass spectrometric (GC-MS) analysis of their permethylated derivatives.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D005965:Glucuronates / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008691:Methadone / Q000378:metabolism*; D051381:Rats; D013237:Stereoisomerism
D005965:Glucuronates; D008691:Methadone