[Study of ribonucleoprotein particles by the method of RNA chromatography on a column of nucleoprotein-celite].
A novel method of RNA fractionation based on a gradual release of the RNA molecules from ribonucleoprotein complexes has been used for the analysis of ribosomal and non-ribosomal complexes of rat liver cytoplasm. Adsorption of native ribonucleoproteins on a Celite column (occuring through only the protein moiety) followed by a consequent dissociation of RNP complexes brought about by various agents results in RNA fractionation in accordance with the tightness of the RNA-protein bonds. The cytoplasmic ribosomal and rapidly labelled non-ribosomal RNA species are separated into several fractions identified as 18S and 28S rRNA's, mRNA and messenger-like RNA. A relatively small fraction (about 10% of the total) of rRNA tenaciously bound to protein has been also revealed.
D000818:Animals; D002845:Chromatography / Q000379:methods; D003593:Cytoplasm / Q000032:analysis; D008099:Liver / Q000032:analysis; D008297:Male; D012313:RNA / Q000032:analysis*; D051381:Rats
D012313:RNA
Issledovanie ribonukleoproteidnykh chastits metodom khromatografii RNK na kolonka nukleoproteid-tselita