title: Induction of globin mRNA accumulation by hemin in cultured erythroleukemic cells.
abstract: The role of heme in erythroid development is investigated in erythroleukemic (Friend) cells. Exogenous hemin induces the accumulation of globin mRNA and globin protein in T3-C12 erythroleukemia cells to levels comparable to those induced by polar solvents, such as dimethylsulfoxide (DMSO). The hemin concentration required for maximal induction (10(-4) M) is the same as that which stimulates globin message translation in reticulocytes or cell-free reticulocyte lysates. Hemin and DMSO together cause T3-C12 cells to accumulate 8-9 fold more globin mRNA than either inducer individually. The kinetics of globin mRNA induction in hemin as compared to DMSO are very different: globin message accumulation begins 4 hr after hemin addition, but not until 30--40 hr after DMSO addition. Biliverdin induces 20--40 fold less hemoglobin than hemin; delta-aminolevulinic acid and porphobilinogen do not induce.
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology; D001664:Biliverdine / Q000494:pharmacology; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004920:Erythropoiesis; D005914:Globins / Q000096:biosynthesis*; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000494:pharmacology*; D006454:Hemoglobins / Q000096:biosynthesis; D007700:Kinetics; D011162:Porphobilinogen / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis*
chemical_list: D006454:Hemoglobins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D006418:Heme; D006427:Hemin; D011162:Porphobilinogen; D000622:Aminolevulinic Acid; D005914:Globins; D001664:Biliverdine; D004121:Dimethyl Sulfoxide