Enrichment of PHA transformed lymphocytes in samples containing mixed populations.
A dextran density gradient centrifugation method has been used to separate PHA transformed lymphocytes from nontransformed lymphocytes obtained from rat blood. Fifty-four percent of the transformed lymphocytes were recovered in the fraction at a density of 1.0620 g/ml. Eighty-five percent of the cells in this fraction were transformed cells while the remainder were non-transformed lymphocytes.
D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008297:Male; D051381:Rats; D013997:Time Factors
D037102:Lectins