A rapid method for the estimation of prostaglandin 15-hydroxydehydrogenase activity and its application to pharmacology.
A novel double-isotope assay for prostaglandin 15-hydroxydehydrogenase (PGDH) is described. 2 The assay is simple, rapid, precise, completely specific for PGDH, and free from artefacts. The results obtained correlate well with a conventional assay. 3 The method is versatile, being applicable to estimations of enzyme activity in cell-free systems as well as perfused organs, and is well suited to the assay of PGDH inhibitors.
D000429:Alcohol Oxidoreductases / Q000032:analysis*; D000818:Animals; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D003513:Cycloheximide / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006911:Hydroxyprostaglandin Dehydrogenases / Q000032:analysis*; D066298:In Vitro Techniques; D007553:Isotope Labeling; D007668:Kidney / Q000201:enzymology / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008722:Methods; D010084:Oxidation-Reduction; D011460:Prostaglandins F / Q000138:chemical synthesis / Q000378:metabolism; D051381:Rats; D013329:Structure-Activity Relationship; D014316:Tritium
D002250:Carbon Radioisotopes; D004791:Enzyme Inhibitors; D011460:Prostaglandins F; D014316:Tritium; D003513:Cycloheximide; D000429:Alcohol Oxidoreductases; D006911:Hydroxyprostaglandin Dehydrogenases