title: The time course of DNA repair following methyl nitro-nitrosoguanidine (MNNG) treatment of p388f lymphoma cells in culture. II. DNA repair synthesis and its correlation with strand breakage.
abstract: Repair synthesis has been followed in P388F cell DNA following treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) using 5-iodo-deoxyuridine (IUdR) and [3H] thymidine combination. A pattern of repair synthesis was obtained, similar in its timing to the initial and medial stages of single-strand break formation and rejoining within the first 24 h after treatment. A higher base insertion frequency in relation to single-strand breaks occurs during the medial stage of repair. However, a fundamental problem with the use of iododeoxyuridine and [3H] thymidine ([3H] TdR) as a suitable measure of repair synthesis was encountered and a potential source of error in such experiments was observed.
mesh_terms: D002460:Cell Line; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D007065:Idoxuridine / Q000494:pharmacology; D007700:Kinetics; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009604:Nitrosoguanidines / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
chemical_list: D004277:DNA, Single-Stranded; D009604:Nitrosoguanidines; D008769:Methylnitronitrosoguanidine; D007065:Idoxuridine; D013936:Thymidine