The effects of photosensitizers and ultraviolet irradiation on the biosynthesis and metabolism of prostaglandins.
Prostaglandin biosynthesis from arachidonic acid by skin microsomal fraction preparation was enhanced by UV-irradiation at wavelengths of 254 and 360 nm. In the presence of 8-methoxy psoralen (8 MOP) and coal tar, prostaglandin biosynthesis was further enhanced approximately 2-fold by UV-irradiation at 254 nm. Stimulation was less by UV-irradiation at 360 nm. 8-MOP enhanced the conversion of PGE2 into PGF2-9-ketoreductase prepared from skin high speed supernatant fractions. UV-irradiation at 254 nm and 360 nm with or without the photosensitizers had no effect on the activity of the PGE2-9-ketoreductase. These data therefore indicate that the action of UV-irradiation, 8-methoxy psoralen and coal tar on the skin may in part be due to their regulation of the biosynthesis and metabolism of prostaglandins in this tissue.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002417:Cattle; D003033:Coal Tar / Q000494:pharmacology; D066298:In Vitro Techniques; D008730:Methoxsalen / Q000494:pharmacology; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D009173:Mycophenolic Acid / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
D001095:Arachidonic Acids; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D003033:Coal Tar; D009173:Mycophenolic Acid; D008730:Methoxsalen