Induction of benzo[a]pyrene Mono-oxygenase in liver cell culture by the photochemical generation of active oxygen species. Evidence for the involvement of singlet oxygen and the formation of a stable inducing intermediate.
1. The photochemical generation of excited states of oxygen in liver cell culture by the mild ilumination of culture medium containing riboflavin, results in stimulation of benzo[a]pyrene 3-mono-oxygenase, a cytochrome P-450-linked mono-oxygenase. 2. The same large increase in mono-oxygenase activity was found when medium containing riboflavin was illuminated in the absence of cells and then stored in the dark for 24h before contact with the cells. From this it may be inferred that stimulation is due to the formation of a stable inducer in the culture medium. Further experiments indicate that the stable inducer is due to the photo-oxidation of an amino acid. 3. Evidence that singlet oxygen is responsible for initiating the stimulation of the mono-oxygenase is based on the use of molecules that scavenge particular active oxygen species. Of all the scavengers tested, only those that scavenge single oxygen inhibited the stimulation. 4. A hypothesis is developed to relate the stimulation of the mono-oxygenase by singlet oxygen in cultured cells to the regulation of the cytochrome P-450 enzyme system in vivo. It is suggested that single oxygen generation within cells may be a common factor linking the many structurally diverse inducers of the enzyme system.
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000494:pharmacology; D000818:Animals; D001372:Aza Compounds / Q000494:pharmacology; D001551:Benz(a)Anthracenes / Q000494:pharmacology; D001579:Benzopyrene Hydroxylase / Q000096:biosynthesis*; D001643:Bridged Bicyclo Compounds / Q000494:pharmacology; D002338:Carotenoids / Q000494:pharmacology; D002478:Cells, Cultured; D004790:Enzyme Induction; D005029:Ethylenediamines / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D008954:Models, Biological; D009580:Nitroblue Tetrazolium; D010100:Oxygen / Q000494:pharmacology*; D010777:Photochemistry; D051381:Rats; D012256:Riboflavin / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology
D001372:Aza Compounds; D001551:Benz(a)Anthracenes; D001643:Bridged Bicyclo Compounds; D005029:Ethylenediamines; D009580:Nitroblue Tetrazolium; D002338:Carotenoids; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D006899:Mixed Function Oxygenases; D001579:Benzopyrene Hydroxylase; D013482:Superoxide Dismutase; D010100:Oxygen; D012256:Riboflavin