Proton magnetic resonance (PMR) studies of water in dimethyl sulfoxide perfused rat myocardium.
Time-dependent studies of the PMR linewidths and spin-lattice relaxation times (T1) of rat myocardium perfused with chemically-defined tissue culture medium containing 15% dimethyl sulfoxide (DMSO) (v/v) were performed. In comparison with "normal" myocardium samples and samples from hearts perfused without DSMO, linewidths initially increased following perfusion of the hearts with 15% DMSO solution. However, the increase was observable only during the first five minutes following perfusion, subsequently narrowing considerably. A minimum T1 was obtained 30 minutes post-treatment. The experiments indicate translocation of intracellular water into bulk-like presumably extracellular water, thus suggesting a cellular dehydration effect of DMSO.
D000818:Animals; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D009206:Myocardium / Q000032:analysis*; D009926:Organ Preservation; D010477:Perfusion; D051381:Rats; D014867:Water / Q000032:analysis*
D003451:Cryoprotective Agents; D014867:Water; D004121:Dimethyl Sulfoxide