Organic solutes in fluid absorption by renal proximal convoluted tubules.
Proximal convoluted tubules were dissected from rabbit kidneys and perfused with artificial solutions in vitro. The effect of various organic solutes on rate of fluid absorption and transepithelial voltage was tested by removing solutes from or adding them to perfusate and/or bath. Omission of albumin from the bath caused rate of fluid absorption to descrease 33% without any change in voltage. Omission of glucose, lactate, alanine, and citrate from the bath had no effect. In contrast, when they were removed from perfusate, rate of fluid absorption fell by 45-75% (depending on whether they were replaced by NaCl or mannitol and NaCl), and voltage (normally negative in lymen) decreased to near zero. Adding glucose or alanine individually to perfusate caused a small increase in rate of fluid absorption and a relatively large increase in voltage. alpha-Methyl-D-glucoside and cycloleucine (which are transported but not metabolized) had effects similar to glucose and alanine, except that voltage changes were not as great. Phlorizin (10(-5) M in perfusate) had the same effect as removing glucose from perfusate. When glucose and alanine were added to perfusate, epithelial cell swelled significantly. Lactate and citrate also caused rate of fluid absorption to increase when they were added to perfusate, but they did not affect transepithelial voltage nor did they cause cells to swell significantly. Possible mechanisms of these effects and the role of organic solutes in fluid absorption by proximal convoluted tubules are discussed.
D000409:Alanine / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002241:Carbohydrates / Q000494:pharmacology; D002264:Carboxylic Acids / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology; D003515:Cycloleucine / Q000494:pharmacology; D004594:Electrophysiology; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D007773:Lactates / Q000494:pharmacology; D008757:Methylglucosides / Q000494:pharmacology; D010695:Phlorhizin / Q000494:pharmacology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D000596:Amino Acids; D002241:Carbohydrates; D002264:Carboxylic Acids; D002951:Citrates; D007773:Lactates; D008757:Methylglucosides; D003515:Cycloleucine; D012710:Serum Albumin, Bovine; D010695:Phlorhizin; D005947:Glucose; D000409:Alanine