Ultrastructural studies of frozen-thawed 8-cell mouse embryos.
Survival of frozen 8-cell mouse embryos transferred directly upon thawing to the uteri of Day 3 pseudopregnant foster mothers was significantly lower (26%) than the survival of unfrozen 8-cell embryos transferred immediately after collection (73%). When frozen-thawed 8-cell embryos were cultured for 20-24 hr before transfer survival was similar to that of unfrozen 8-cell embryos transferred after 20-24 hr inculture (65% and 73%, respectively). Ultrastructural examination of the frozen-thawed 8-cell embryos revealed no obvious damage to protoplasmic components.
D000818:Animals; D046508:Culture Techniques; D003593:Cytoplasm / Q000648:ultrastructure; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000648:ultrastructure*; D005260:Female; D005615:Freezing; D051379:Mice; D011247:Pregnancy; D013997:Time Factors; D014021:Tissue Preservation; D014022:Tissue Survival