Formation of extracellular protein A by Staphylococcus aureus.
Staphylococcus aureus contains cell wall protein A as well as extracellular protein A. The two types of protein A have very similar amino acid compositions, electrophoretic mobilities and sizes. The release of extracellular protein A from exponentially growing bacteria is dependent on protein synthesis de novo and protein A is released directly after being synthesized on the ribosomes. Bacteria in the stationary phase, however, release protein A as a result of cell lysis. Protoplasts have been isolated which produce protein A as extensively as the intact bacteria but because of the absence of of the formation of extracellular protein A is observed from cells also producing cell wall protein A.
D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008239:Lysine / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000187:drug effects / Q000378:metabolism*
D000596:Amino Acids; D001426:Bacterial Proteins; D011691:Puromycin; D008239:Lysine