A rapid, manual test for amylase at 52C.
A simple modification of an alpha-amylase (E.C. 3.2.1.1.) procedure, using blue starch substrate, permits quantitative results after three minutes of incubation. The procedure, performed on blank, control, and unknown speciemns, takes advantage of the heat stability of the enzyme and provides immediate and reliable data for acutely ill patients. The performance characteristics of the test on serum and urine are described. The method is distinctly advantageous in prividing rapid results for serum amylase, urine amylase, and amylase clearance in patients with suspected pancreatitis.
D000681:Amylases / Q000032:analysis* / Q000097:blood / Q000652:urine; D004796:Clinical Enzyme Tests; D006358:Hot Temperature; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007700:Kinetics; D008722:Methods; D010179:Pancreas / Q000201:enzymology; D010195:Pancreatitis / Q000175:diagnosis; D012463:Saliva / Q000201:enzymology; D013997:Time Factors
D007527:Isoenzymes; D000681:Amylases