title: The interactions between iophenoxic acid, iopanoic acid, bilirubin and human serum albumin as studied by fluorescence and Sephadex gel filtration.
abstract: Iophenoxic acid increases the fluorescence of bilirubin bound to human serum albumin at drug/albumin molar ratios lower than 1, while iopanoic acid decreases it. The fluorescence enhancement results probably from a change in the fluorescence efficiency due to an iophenoxic acid-induced conformational change in the albumin, which in turn causes displacement of bilirubin from the protein. Iophenoxic acid does not affect the high-affinity bilirubin binding site of albumin. Therefore any enhancement in bilirubin fluorescence caused by the drug indicates that bilirubin is bound to the low-affinity binding sites of albumin. The use of iophenoxic acid in the determination of the extent of saturation of the high-affinity bilirubin binding site of albumin may be of value in the clinical management of infants with neonatal jaundice.
mesh_terms: D001663:Bilirubin; D002850:Chromatography, Gel; D002934:Cinnamates; D006801:Humans; D007462:Iodobenzenes; D007480:Iopanoic Acid / Q000031:analogs & derivatives*; D011485:Protein Binding; D012709:Serum Albumin; D013050:Spectrometry, Fluorescence
chemical_list: D002934:Cinnamates; D007462:Iodobenzenes; D012709:Serum Albumin; C008160:iophenoxic acid; D007480:Iopanoic Acid; D001663:Bilirubin