Studies on delayed hypersensitivity of antigens isolated from Mycoplasma pneumoniae cells.
Cells of Mycoplasma pneumoniae Mac strain were fractionated into acetone-soluble and insoluble fractions. Acetone-insoluble fractions were digested with pronase and further purified by chromatography on Sephadex G-75, yielding three water-soluble fractions which were free from lipid and consisted mainly of polysaccharide-protein complex. All these water-soluble fractions possessed eliciting antigenicity to delayed hypersensitivity for M. pneumoniae as measured by skin reactions and macrophage migration inhibition tests, but not to complement-fixing antibodies. In contrast, the acetone-soluble fraction was reactive with the complement-fixing antibodies but not for the delayed hypersensitivity.
D000096:Acetone; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002464:Cell Migration Inhibition; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D008055:Lipids / Q000032:analysis; D008264:Macrophages / Q000276:immunology; D009174:Mycoplasma / Q000276:immunology*; D010758:Phosphorus / Q000032:analysis; D012882:Skin Tests; D012995:Solubility; D014867:Water
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002241:Carbohydrates; D008055:Lipids; D014867:Water; D000096:Acetone; D010758:Phosphorus