title: Solubilization of IgA immune precipitates by complement.
abstract: The complement (C) system can solubilize immune precipitates prepared with antibodies of the IgG class, and apparently also of the IgM class. This paper shows that C can also solubilize immune precipitates prepared with two different antigen-binding IgA myeloma proteins. Immune precipitates were prepared with a) mouse myeloma protein TEPC 15 and PC-KLH, and b) mouse myeloma protein MOPC 315 and DNP-BSA. The precipitates could be solubilized by fresh mouse serum, but not by zymosan- or heat-inactivated serum. The rate of solubilization was not affected by the removal of of Ca++ ions. These results verify that the C-mediated solubilization effect can proceed entirely via the alternative C pathway, and lend increased support to the view that the effect is a general phenomenon, not restricted to a particular antibody class or type of antigen.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent; D003165:Complement System Proteins / Q000378:metabolism*; D006433:Hemocyanins / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D007070:Immunoglobulin A / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000276:immunology; D009578:Nitrobenzenes / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D012995:Solubility
chemical_list: D000936:Antigen-Antibody Complex; D002413:Cations, Divalent; D007106:Immune Sera; D007070:Immunoglobulin A; D009194:Myeloma Proteins; D009578:Nitrobenzenes; D012710:Serum Albumin, Bovine; D003165:Complement System Proteins; D006433:Hemocyanins; D008274:Magnesium; D002118:Calcium