Prostaglandin production by methylcholanthrene-transformed mouse BALB/3T3: inhibition by cytochalasin B.
Cytochalasin B inhibits the production of prostaglandins by serum-, thrombin-, and bradykinin-stimulated MC5-5 cells. The serum-stimulated release of arachidonic acid from cellular phospholipids also is inhibited. Cytochalasin B does not affect the cells' prostaglandin synthetase activity when exogenous arachidonic acid is present. Deacylation of phospholipids may be the step affected by cytochalasin B possibly as a result of disruption of microfilament organization. Colchicine and vinblastine, two drugs that can disrupt microtubule organization, do not inhibit prostaglandin production by cells.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D001920:Bradykinin / Q000494:pharmacology; D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology*; D003572:Cytochalasins / Q000494:pharmacology; D004347:Drug Interactions; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011453:Prostaglandins / Q000096:biosynthesis*; D013917:Thrombin / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology
D001095:Arachidonic Acids; D003572:Cytochalasins; D011453:Prostaglandins; D003571:Cytochalasin B; D008748:Methylcholanthrene; D014747:Vinblastine; D013917:Thrombin; D001920:Bradykinin; D003078:Colchicine