Gamma-aminobutyric acid release from neurons and glia.
Release of 3H-GABA into the medium from bulk-isolated glial cells, neuronal perikarya and synaptosomes has been studied, using a continuous perfusion system. The fractions were incubated with 3H-GABA and spontaneous efflux of label in the presence and absence of aminooxyacetic acid (AOAA) was recorded. Unlabelled GABA at micromolar concentrations markedly stimulated 3H-GABA efflux from glia, synaptosomes and neurons. 3H-GABA from all fractions was also markedly sensitive to stimulation by ouabain. Glutamate was effective in stimulating the spontaneous efflux in the presence of AOAA, while superfusion with a calcium-deprived medium only slightly stimulated the 3H-GABA efflux from neurons and glia.
D000613:Aminobutyrates / Q000378:metabolism*; D000625:Aminooxyacetic Acid / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D010477:Perfusion; D011817:Rabbits; D012636:Secretory Rate / Q000187:drug effects; D013574:Synaptosomes / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000494:pharmacology
D000613:Aminobutyrates; D005971:Glutamates; D000625:Aminooxyacetic Acid; D005680:gamma-Aminobutyric Acid; D010042:Ouabain; D002118:Calcium