Limitations of the direct linear plot in evaluation of drug-protein binding parameters.
The applicability of the direct linear plot is compared with that of the Scatchard plot for the estimation of protein binding parameters. Only, if one class of binding sites exists in the system tested, binding parameters may be estimated by use of the direct linear plot. On the other hand the Scatchard plot also provides estimates in systems with more that one class of binding sites.
D001665:Binding Sites; D007700:Kinetics; D008433:Mathematics; D011485:Protein Binding