Fine structure of 33 258 H-treated chromosomes.
Metaphase chromosomes of mouse strain L cells show strikingly uncondensed pericentric heterochromatic regions after treatment of living cells with the benzimidazol-derivative 33 258 Hoechst. In electron micrographs of total preparations after G-band staining the chromosomes are seen to be made up of irregularly folded fibrils of 200-400 A in diameter. In the uncondensed regions only very few fibrils laid loose loops are present, making it probable that only one fibril forms one chromatid.
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002875:Chromosomes / Q000187:drug effects* / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006570:Heterochromatin / Q000378:metabolism; D007739:L Cells; D051379:Mice
D001562:Benzimidazoles; D005456:Fluorescent Dyes; D006570:Heterochromatin