title: Optimum amino acid complement for protein synthesis by rat mammary cells in tissue culture.
abstract: The amino acid requirement of rat mammary cells for milk protein synthesis was investigated in dispersed cell culture. A three-dimensional central composite design utilizing three variables (X1 = lysine; X2= methionine, valine, and arginine; X3 = isoleucine, tryptophan, threonine, phenylalanine, and histidine) at five concentrations each, was duplicated twice with mammary cells from lactating Sprague-Dawley rats. The optimum combination of amino acids for maximum milk protein synthesis from multiple regression models was X1 15.0-, X2 4.5-, and X3 1.5-fold their quantities in Eagle's minimal essential medium with leucine, tyrosine, cystine, and glutamine at the base 1-fold in the medium.
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002364:Caseins / Q000096:biosynthesis; D002478:Cells, Cultured; D005260:Female; D007768:Lactalbumin / Q000096:biosynthesis; D007774:Lactation; D007782:Lactoglobulins / Q000096:biosynthesis; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008433:Mathematics; D008894:Milk Proteins / Q000096:biosynthesis*; D011247:Pregnancy; D051381:Rats
chemical_list: D000596:Amino Acids; D002364:Caseins; D007782:Lactoglobulins; D008894:Milk Proteins; D007768:Lactalbumin