Fluorometric method for assay of kallikrein-like arginine esterases.
The fluorometric method of Nash for determination of methanol was applied in assay of kallikrein-like arginine esterases using N-a-tosyl-L-arginine methyl ester (TAME) as substrate. The results obtained using this method corresponded well with those obtained in the usual colorimetric methods using hydroxamate, chromotropic acid, and 3-methyl-2-benzothiazolone hydrazone and the present method was more sensitive than these colorimetric methods.
D001120:Arginine; D004950:Esterases / Q000032:analysis* / Q000378:metabolism; D005069:Evaluation Studies as Topic; D007610:Kallikreins / Q000032:analysis* / Q000378:metabolism; D007700:Kinetics; D013050:Spectrometry, Fluorescence / Q000379:methods
D001120:Arginine; D004950:Esterases; D007610:Kallikreins