PHA response and methylazoxymethanol acetate.
Several reports have indicated the mutagenicity of the cycasin aglycone, methyl azoxymethanol (MAM). Van Den Berg and Ball (1972) have demonstrated the inhibitory effects of MAM acetate (MAMac), a more stable form of the aglycone, on DNA synthesis and cell proliferation in HeLa cells. The purpose of this study was to observe the effects of MAMac on blastogenesis in the human short-term leucocyte culture system. A depression in blastogenesis by MAMac was observed cytologically derived from two individuals. The same effect was observed utilizing [3H] thymidine as an indicator of blastogenesis in a series of cultures from 11 male individuals exposed to varying doses of MAMac, ranging from 5 through 800 mug/ml.
D001391:Azo Compounds / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D007962:Leukocytes / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008746:Methylazoxymethanol Acetate / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D001391:Azo Compounds; D037102:Lectins; D008746:Methylazoxymethanol Acetate; D013936:Thymidine