Mass spectrometric investigation of peptide amides.
The reaction products of trifluoroacetic anhydride with peptide amides are identified as stable heterocyclic imidazolin-5(4)-ones, which can be analysed by gas chromatography mass spectrometry. The mass spectra show intense molecular ions and allow the assignment of the amino acid sequence.
D000107:Acetylation; D000577:Amides / Q000032:analysis*; D002849:Chromatography, Gas; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis*; D012972:Sodium Hydroxide; D014269:Trifluoroacetic Acid
D000577:Amides; D010455:Peptides; D012972:Sodium Hydroxide; D014269:Trifluoroacetic Acid