title: The activity of delta 5-3 beta hydroxysteroid dehydrogenase enzyme in the interrenal tissue of rainbow trout (Salmo gairdneri Richardson) exposed to sublethal concentrations of zinc.
abstract: The effects of exposure of rainbow trout (Salmo gairdneri Richardson) to three sublethal concentrations of zinc (0.248, 0.528 and 1.14 ppm) on delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta HSDH) enzyme activity in the head kidney tissue was investigated. The activity of this enzyme was localized by the reduction of a tetrazolium salt to formazan at the site of activity. Using subjective methods to indicate the degree of activity and indirectly assess the degree of corticosteroid production, the zinc-exposed rainbow trout showed a greater degree of delta 5-3 beta HSDH activity compared to the control fish. This increase in enzyme activity has been associated with the stimulation of the pituitary-interrenal axis by the noxious stress of zinc.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D005618:Fresh Water / Q000032:analysis; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D010100:Oxygen / Q000032:analysis; D012487:Salmonidae / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology*
chemical_list: D000429:Alcohol Oxidoreductases; D006911:Hydroxyprostaglandin Dehydrogenases; D015032:Zinc; D010100:Oxygen