Cytogenetic effects of mutagens/carcinogens after activation in a microsomal system in vitro I. Induction of chromosome aberrations and sister chromatid exchanges by diethylnitrosamine (DEN) and dimethylnitrosamine (DMN) in CHO cells in the presence of rat-liver microsomes.
A rat-liver microsomal system in vitro has been used to activate two indirectly acting carcinogens, DMN and DEN. On activation, both compounds were extremely potent in inducing chromosomal aberrations as well as sister chromatid exchanges in Chinese hamster cells. The implications of these findings and the potential utility of this technique to detect mutagens/carcinogens are discussed.
D002273:Carcinogens / Q000494:pharmacology; D002460:Cell Line; D002842:Chromatids / Q000187:drug effects; D002869:Chromosome Aberrations; D004052:Diethylnitrosamine / Q000494:pharmacology*; D004128:Dimethylnitrosamine / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000378:metabolism*; D009153:Mutagens / Q000494:pharmacology; D009602:Nitrosamines / Q000494:pharmacology*
D002273:Carcinogens; D009153:Mutagens; D009602:Nitrosamines; D004052:Diethylnitrosamine; D004128:Dimethylnitrosamine