Cholera toxin-peroxidase: changes in surface labeling of glioblastoma cells with increased time in tissue culture.
Cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. Studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides.
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D010544:Peroxidases; D013997:Time Factors; D014118:Toxins, Biological / Q000378:metabolism; D014734:Vibrio cholerae
D005732:Gangliosides; D014118:Toxins, Biological; D010544:Peroxidases