Chloramphenicol-induced loss of mitochondrial DNA polymerase activity in HeLa cells.
HeLa cells exposed to chloramphenicol for approximately one cell generation were found to contain a mitochondria-associated DNA polymerase with a significantly lower specific activity than that of control cells. This observation was not due to the presence of inhibitors in mitochondrial DNA polymerase preparations of chloramphenicol-treated cell cultures. In addition, there was no accumulation of a typical mitochondrial DNA polymerase in the post-mitochondrial supernatant of drug-treated cells.
D002701:Chloramphenicol / Q000494:pharmacology*; D003593:Cytoplasm / Q000187:drug effects / Q000201:enzymology; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000378:metabolism*; D006367:HeLa Cells / Q000201:enzymology*; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*
D002701:Chloramphenicol; D004259:DNA-Directed DNA Polymerase