title: Identification of specific changes in the pattern of brain protein synthesis after training.
abstract: Double labeling studies with [3H]valine and [14C]valine were used to investigate the pattern of protein synthesis in the brains of goldfish. The protein fractions in three bands (alpha, beta, and gamma) on sodium dodecyl sulfate-polyacrylamide gels indicate that more valine was incorporated in the brains of goldfish that had been trained in a vestibular conditioning task than in the brains of untrained fish or fish trained in a variety of control behavioral situation. Changes in the pattern of labeling were localized in the cytoplasmic fraction of the brain; no increases in labeling occurred in either the nuclear or synaptosomal components. The results suggest that a specific change occurs in the pattern of protein synthesis in the brain after the acquistion of a new behavior.
mesh_terms: D000367:Age Factors; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D003213:Conditioning, Psychological / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D006054:Goldfish; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D013312:Stress, Physiological / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins