Synthesis of the brain-specific S-100 protein in a cell-free system from wheat embryo programmed with poly(A)-containing RNA from rabbit brain.
Polyadenylated polysomal RNA was prepared from rabbit cerebral hemispheres using phenol extraction and chromatography on oligo(dT)-cellulose. This RNA directed the synthesis of the brain-specific S-100 protein in cell-free extracts from wheat embryo. S-100 protein was absent from the products of endogenous incorporation and from a reaction programmed with kidney mRNA. These results suggest that S-100 protein mRNA contains a poly(adenylic acid) sequence and rule out the necessity of a brain-specific factor for translation of -S100 protein mRNA.
D000818:Animals; D001923:Brain Chemistry; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010944:Plants / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism*; D011132:Polyribosomes; D011189:Potassium Chloride / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D009418:S100 Proteins / Q000096:biosynthesis*; D014908:Triticum / Q000378:metabolism
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D009418:S100 Proteins; D011061:Poly A; D011189:Potassium Chloride