Synthesis and purification of radioactive 6beta-iodomethyl-19-norcholest-5(10)-en-3beta-ol.
A method for the synthesis and purification of 6beta-iodomethyl-19-norcholest-5(10)-ene-3beta-ol-131I of greater than 98 mole% chemical purity and greater than 99% radiochemical purity is presented. Carbon-13 and proton NMR were used to establish the identity and purity. Discrepancies in the characterization of this compound, previously published by two other research groups, are discussed.
D002782:Cholestenes / Q000138:chemical synthesis; D002855:Chromatography, Thin Layer; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D013261:Sterols / Q000138:chemical synthesis*
D002782:Cholestenes; D007457:Iodine Radioisotopes; D013261:Sterols