title: D-Glucose enhancement of water reabsorption in proximal tubule of the rat kidney.
abstract: Water reabsorption in the proximal convoluted tubule of the rat kidney was examined by in vivo microperfusion techniques in order to examine the effect of D-glucose within the tubular lumen. When tubules were perfused with a balanced artificial solution containing Na, K, Cl, HCO3, urea, and D-glucose, absolute reabsorption averaged 4.01 +/- 0.24 nl/min per mm. Addition of D-glucose to the NaCl perfusate enhanced water reabsorption to values similar to those obtained with the balanced artificial perfusate. The enhanced water reabsorption consequent to the addition of D-glucose to the NaCl perfusion solution was completely inhibited by addition of phloridzin to the perfusate. The addition of an unabsorbed hexose, 2-deoxy-D-glucose, to the NaCl perfusate failed to enhance water reabsorption, whereas the addition of an incompletely reabsorbed sugar that is not metabolized, 3-O-methyl-D-glucose, resulted in partial enhancement of theabsolute rate of water reabsorption. These studies demonstrate that D-glucose has the specific effect of augmenting water reabsorption in the proximal tubule of the rat kidney.
mesh_terms: D000818:Animals; D003847:Deoxyglucose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000502:physiology*; D008297:Male; D008757:Methylglucosides / Q000494:pharmacology; D010695:Phlorhizin / Q000494:pharmacology; D051381:Rats; D012965:Sodium Chloride; D014882:Water-Electrolyte Balance
chemical_list: D008757:Methylglucosides; D012965:Sodium Chloride; D003847:Deoxyglucose; D010695:Phlorhizin; D005947:Glucose