Invertase biosynthesis by Saccharomyces carlsbergensis in batch and continuous cultures.
The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis at a particular intermediate growth rate. The value of E/X increased from 1.1 (U/mg biomass) in batch cultures to 13 (U/mg biomass) in chemostat cultures. A mutant strain A3 showed the highest value for E/X = 25 (U/mg biomass) at high dilution rates where glucose repression was observed with the wild strain.
D004794:Enzyme Repression; D005947:Glucose / Q000494:pharmacology; D008828:Microbiological Techniques; D009154:Mutation; D010100:Oxygen / Q000378:metabolism; D012440:Saccharomyces / Q000187:drug effects / Q000378:metabolism*; D013393:Sucrase / Q000096:biosynthesis* / Q000378:metabolism
D013393:Sucrase; D005947:Glucose; D010100:Oxygen