Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2.
Fruit bromelain FA2, the main proteinase component of the juice of pineapple fruit, has been purified and characterized. 1. Efficient extraction of this enzyme from the crude material was possible using "Cellulosin AP," a microbial polysaccharidase preparation containing cellulase, hemicellulase, and pectinase. The enzyme was purified mainly by successive applications of anion-exchange chromatography, yielding an apparently homogeneous protein as judged by several physical, chemical, and immunochemical criteria. Properties of FA2 include: molecular weight, 31,000; isoelectric point, pH 4.6; absorbance at 280 nm of a 1% solution at pH 7.0 per cm, 19.2. 2. FA2 gave only alanine phenylthiohydantoin upon amino-terminal group analysis by the Edman procedure. Stepwise degradation yielded the amino-terminal sequence Ala-Val-Pro-Gln-Ser-Ile-Asp-Trp-Arg-Asp-Tyr-Gly-Ala. The amino acid composition of FA2 was not markedly different from that of stem bromelain, except for a much smaller lysine content and a smaller alanine content relative to glycine in FA2. FA2 contained neither amino sugars nor neutral carbohydrates as determined by several methods, so FA2 is not a glycoprotein. 3. By labeling the reactive cysteine residue (CYS) with [14C]iodoacetate, the following partial amino acid sequence has been determined. Asn-Glx-Asn-Pro-Cys-Gly-Ala-CYS.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D001920:Bradykinin / Q000378:metabolism; D001963:Bromelains / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D005944:Glucosamine / Q000032:analysis; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D010944:Plants / Q000201:enzymology; D051381:Rats; D014599:Uterus / Q000378:metabolism
D000596:Amino Acids; D000804:Angiotensin II; D001963:Bromelains; D005944:Glucosamine; D001920:Bradykinin