Establishment of a cell line (Co-115) from a human colon carcinoma transplanted into nude mice.
A human colon carcinoma cell line, Co-115, has been established in vitro from solid xenografts maintained in nude mice and subcultured for 95 passages. Co-115 cells grow in vitro as tightly packed, epithelial-like colonies, have a doubling time of about 36 hr, have a relatively low plating efficiency in agar, and release significant amounts of carcinoembryonic antigen to the culture medium. Their epithelial nature has been confirmed by ultrastructural examination. The injection of Co-115 cells into nude mice reinduced the formation of solid tumor masses that could be retransplanted and showed a morphology comparable of that of the original xenograft.
D000230:Adenocarcinoma / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D003110:Colonic Neoplasms / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
D002272:Carcinoembryonic Antigen