[Extraction and properties of microcapsulated alpha-chymotrypsin].
A method of microencapsulating of the proteolytic enzyme alpha-chymotrypsin into semi-permeable nylon membranes is worked out. The membrane is a polimer of 1,6-hexamethylenediamine and sebacoyl chloride. alpha-Chymotrypsin is enclosed into the capsule together with polyethyleneimine, capable of joining the walls of microcapsules and making the membrane more stable. The optimal concentrations of polyenthyleneimine and alpha-chymotrypsin are 5% and 1% correspondingly. The highest yield of microencapsulated enzyme was obtained for completely acetylated delta-chymotrypsin. The kinetic properties of microencapsulated alpha-chymotrypsin change very slightly as compared to those of the native one.
D000107:Acetylation; D002918:Chymotrypsin / Q000302:isolation & purification; D004800:Enzymes, Immobilized; D011094:Polyethyleneimine
D004800:Enzymes, Immobilized; D011094:Polyethyleneimine; D002918:Chymotrypsin
Poluchenie i svoĭstva mikrokapsulirovannogo alpha-khimotripsina