[The influence of a metabolic stimulator on the vitality of preserved human corneas (author's transl)].
20 human donor corneas were placed in a tissue culture medium (TC 199 + 5% Dextran = MK-Medium) at +4 degrees C for 5 and 7 days. After that the temperature reversal effect was measured and the corneal endothelial cells were stained. The living cells were counted every 24 hours until no more cells could be seen. Another group of 20 human donor eyes were placed in to the same medium with a additional content of 5% Actihaemyl resp. Solcoseryl. Same procedure as above. It could be shown that under the influence of the metabolic activator 22.0% and 23.3% more living endothelial cells could be counted and that the total survival time could be prolonged by 1 1/2 days.
D000183:Actihaemyl / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D003315:Cornea / Q000187:drug effects*; D046508:Culture Techniques; D006801:Humans; D013696:Temperature; D013997:Time Factors
D000183:Actihaemyl
Der Einfluss eines Stoffwechselaktivators auf die Vitalität konservierter menschlicher Hornhäute