High-performance liquid chromatography of proteins.
Proteins were separated on microparticulate bonded phase steric exclusion and anion-exchange chromatography supports. A post-column enzyme detector was developed which gives a specific and sensitive response for enzymes. The three iso-enzymes of creatine phosphokinase were separated and assayed in 4 min and the five isoenzymes of lactic dehydrogenase in 6 min.
D000818:Animals; D000837:Anion Exchange Resins; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003402:Creatine Kinase / Q000032:analysis*; D006801:Humans; D007422:Intestines / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008099:Liver / Q000032:analysis; D051381:Rats; D013154:Spleen / Q000032:analysis; D013950:Thymus Gland / Q000032:analysis; D013997:Time Factors
D000837:Anion Exchange Resins; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase