Cell-mediated immunity to Vibrio cholerae with ribonucleic acid-protein fractions of V. cholerae L-form lysates.
Different L-form lysate vaccines of Vibrio cholerae serotypes Ogawa and Inaba and their combination along with ethyl alcohol-precipitated ribonucleic acid (E-RNA) and phenol-extracted RNA (P-RNA) fractions of V. cholerae Ogawa lysates were tested for production of cell-mediated immunity. Both E-RNA and P-RNA fractions induced an increase in leukocyte migration inhibition, macrophage migration inhibition, and macrophage aggregation. They also induced delayed hypersensitivity in rabbits. More consistent results were obtained with the P-RNA fraction.
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D002464:Cell Migration Inhibition; D022121:Cholera Vaccines / Q000032:analysis / Q000494:pharmacology; D006968:Hypersensitivity, Delayed / Q000175:diagnosis; D007111:Immunity, Cellular; D007740:L Forms / Q000276:immunology*; D008264:Macrophages; D008297:Male; D012313:RNA / Q000276:immunology*; D011817:Rabbits; D014734:Vibrio cholerae / Q000276:immunology*
D001426:Bacterial Proteins; D022121:Cholera Vaccines; D012313:RNA