Valyl-transfer RNA formation stimulated by monovalent cations and spermine in rat liver.
Valyl-tRNA synthetase from rat liver catalyzes the aminoacylation of tRNA in the presence of a) Mg2+, b) monovalent cations and c) spermine. The degree of aminoacylation with Na+ is equal to that with Mg2+ but the ATP required with Na+ is 25 times lower than that with Mg2+. The aminoacylation becomes almost undetectable in the presence of Na+ when tRNA deprived of Mg2+ is used.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000641:Ammonia / Q000494:pharmacology*; D000818:Animals; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology*; D012343:RNA, Transfer / Q000096:biosynthesis*; D051381:Rats; D012964:Sodium / Q000494:pharmacology*; D013096:Spermine / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*; D014633:Valine
D013096:Spermine; D000641:Ammonia; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D012964:Sodium; D014633:Valine; D008274:Magnesium; D011188:Potassium