The role of protein synthesis in the stimulation by LH of prostaglandin accumulation in rat preovulatory follicles in vitro.
Preovulatory follicles isolated from immature rats, treated in vivo with pregnant mare's serum gonadotropin, were incubated in vitro and the accumulation of prostaglandin E measured. The addition of luteinizing hormone (5 mug/ml) increased this accumulation, after a lag period of 3 hours. This delay suggested the involvement of macromolecular synthesis in the mechanism of prostaglandin stimulation by luteinizing hormone. When the synthesis of protein was inhibited by the addition of puromycin (100 muM), the luteinizing hormone stimulation of prostaglandin E in these follicles was completely abolished. This inhibition was not seen with an analogue of puromycin, which does not inhibit protein synthesis, puromycin amino-nucleoside. These data suggest that concomitant protein synthesis is required for the luteinzing hormone stimulation of prostaglandin accumulation in rat follicles.
D000818:Animals; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism*; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D011692:Puromycin Aminonucleoside / Q000494:pharmacology; D051381:Rats; D013268:Stimulation, Chemical
D006064:Gonadotropins, Equine; D011458:Prostaglandins E; D011691:Puromycin; D011692:Puromycin Aminonucleoside; D007986:Luteinizing Hormone