Isolation of (copper, zinc)-thioneins from the livers of copper-injected rats.
The mixed copper- and zinc-binding proteins, with mol.wt. of approx. 12000, induced in rat liver after injection of copper were isolated and characterized as metallothioneins. Three separate forms were obtained, with 7-11% total metal in the protein, equivalent to 2.6-1.6 cysteine residues/metal atom.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003300:Copper; D008099:Liver / Q000032:analysis*; D008297:Male; D008667:Metalloproteins / Q000302:isolation & purification*; D008668:Metallothionein / Q000302:isolation & purification*; D051381:Rats; D015032:Zinc
D000596:Amino Acids; D008667:Metalloproteins; D003300:Copper; D008668:Metallothionein; D015032:Zinc