[Elimination of a paramyxo-type virus from oncornavirus-producing human cell lines (author's transl)].
A virus of the paramyxo-type was eliminated from cell-free material of human oncornavirus-producing cell lines (PMF). After transmission of this paramyxovirus-free inoculum to a human permanent cell strain (Tu 197/Tr 1) oncornaviruses were permanently formed and no paramyxoviruses could be detected. The paramyxovirus-free, oncornavirus-producing PMF-39 cell line could be established after inoculation of the TU 197/Tr 1 line with cell-free material containing both oncorna- and paramyxovirus diluted 1 to 1000. A second way of elimination of the paramyxovirus was the treatment of cell-free material containing both viruses with antisera against paramyxovirus. In the Tu 197/Tr 1 line inoculated with such material only oncornaviruses were formed. The second paramyxovirus-free oncornavirus-producing cell line was designated PMF 50.
D000818:Animals; D000914:Antibodies, Viral; D000917:Antibody Formation; D002460:Cell Line; D002474:Cell-Free System; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera; D007181:Inclusion Bodies, Viral; D009858:Oncogenic Viruses / Q000276:immunology / Q000502:physiology*; D010252:Paramyxoviridae / Q000276:immunology / Q000502:physiology*; D014779:Virus Replication
D000914:Antibodies, Viral; D007106:Immune Sera
Eliminierung eines Paramyxovirus aus Oncornavirus-produzierenden menschlichen Zellinien