title: The human leukocyte test system. IX. DNA synthesis and mitoses in PHA-stimulated 2-day cultures set up with Eagle's mimimal essential medium (MEM).
abstract: In human leukocyte cultures set up with Eagle's MEM and stimulated with Difco's PHA M, DNA synthesis and mitotic indices were analyzed by means of 3(H)-thymidine autoradiography and cell counting from 23 up to 52 h after culture initiation. Considerable amounts of DNA synthesis and mitoses were found in this time span. This resembles the results found with Ham's F-10 medium. However, the DNA synthesis pattern and the distribution of mitotic indices a higher yield of asynchrony in Eagle's MEM as compared with Ham's F-10 cultures. Proportions of first, second, and third mitoses at 72 h culture time were determined with different methods.
mesh_terms: D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D037102:Lectins; D007962:Leukocytes / Q000166:cytology*; D008938:Mitosis; D008940:Mitotic Index
chemical_list: D003470:Culture Media; D037102:Lectins; D004247:DNA