Isolation of a new lipolytic-melanotropic peptide from human pituitary glands.
A new peptide having both lipolytic and melanotropic properties has been isolated from human pituitary glands. It has a molecular weight around 11,000, an amino acid composition different from the known lipolytic-melanotropic hormones, and an isoelectric point of 8.5. Although it is not entirely pure, there is no doubt that it differs from other known lipolytic-melanotropic substances in its total lack of methionine and tryptophan and its unusually high content of lysine and histidine. It has a melanocyte-stimulating hormone activity of 19 units/mug. As a lipolytic agent it is active with rabbit adipocytes, only slightly active with human cells and inactive with adipocytes from the rat.
D000409:Alanine; D001681:Biological Assay; D002845:Chromatography; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D050356:Lipid Metabolism; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification*; D010902:Pituitary Gland / Q000032:analysis*; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D010908:Pituitary Hormones, Anterior / Q000032:analysis / Q000302:isolation & purification*; D011863:Radioimmunoassay; D012880:Skin Pigmentation; D014364:Tryptophan / Q000032:analysis
D010455:Peptides; D010908:Pituitary Hormones, Anterior; D014364:Tryptophan; D000409:Alanine