Alkali-labile oligosaccharides from glycoproteins of different erythrocyte and milk fat globule membranes.
Phenol extraction of horse, sheep, cow, pig and human erythrocyte membranes and human milk fat globule membranes gave glycoprotein fractions, all of which were shown by gas chromatography to contain the reduced disaccharide beta-D-galactosyl (1-3)-N-acetyl-D-galactosaminital after treatment with alkaline borohydride. Cow and pig erythrocyte membrane glycoproteins were found however to contain much lower amounts than the erythrocyte membrane glycoproteins of the other species tested. After gel filtration, a tetrasaccharide was isolated from horse and sheep glycoproteins containing the disaccharide plus two molecules of sialic acid. Periodate oxidation together with paper chromatography of alkaline degraded fragments showed these two molecules of sialic acid to be linked to positions C3 and C6 of the galactosyl and N-acetylgalactosamine residues respectively. Evidence was obtained for a similar structure from pig and cow erythrocyte glycoproteins and human milk fat globule membrane glycoproteins although the complete structure was not elucidated. In all native glycoprotein fractions, the unsubstituted disaccharide beta-D-galactosyl (1-3)-N-acetyl-D-galactosamine was found to be present to different extents. Haemagglutination inhibition tests against human anti-T serum, Arachis hypogoea and Vicia graminea by desialylated glycoproteins showed the presence of the T-antigen, confirming the chemical findings. Inhibition was found to be proportional to the chemically detected amounts of disaccharide in each fraction. Evidence for a second carbohydrate chain in horse, sheep and human erythrocyte glycoproteins with a sialic acid substituted N-acetylgalactosamine residue as the terminal sequence was obtained using the agglutinin from Helix pomatia.
D000818:Animals; D002417:Cattle; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D006385:Hemagglutination Inhibition Tests; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006736:Horses; D006801:Humans; D008894:Milk Proteins; D008895:Milk, Human / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis; D011247:Pregnancy; D012756:Sheep; D012794:Sialic Acids / Q000032:analysis; D013045:Species Specificity; D013552:Swine
D006023:Glycoproteins; D006595:Hexosamines; D006601:Hexoses; D008894:Milk Proteins; D009844:Oligosaccharides; D012794:Sialic Acids