A sensitive isotopic assay method for S-adenosylhomocysteine hydrolase. Some properties of the enzyme from rat liver.
A rapid and sensitive isotopic method is presented for the assay of S-adenosylhomocysteine hydrolase (EC 3.3.1.1) activity, based on the formation of radioactive S-adenosylhomocysteine labelled in the adenosine portion. The radioactive product is separated either by low-voltage paper electrophoresis or by using phosphocellulose ion-exchange paper. Some kinetic properties of the enzyme from rat liver have shown to be clearly different from those reported earlier for this enzyme. The use of erythro-9-(2-hydroxy-3-nonyl)adenine, a potent inhibitor  of adenosine deaminase, makes it possible to measure the S-adenosylhomocysteine hydrolase activity in tissues with a high adenosine deaminase activity, e.g. in intestinal mucosa.
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D002250:Carbon Radioisotopes; D004590:Electrophoresis, Paper; D005260:Female; D006710:Homocysteine / Q000494:pharmacology; D006867:Hydrolases / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D051381:Rats
D002250:Carbon Radioisotopes; D006710:Homocysteine; D006867:Hydrolases; D000241:Adenosine