Tissue culture, protein and collagen synthesis in antibiotic sterilized canine heart valves.
Viability of canine heart valve leaflet fibroblasts was assessed after varying periods of sterilization and storage in antibiotic-nutrient solution. Tissue culture and assessment of protein and collagen synthesis showed that tissue obtained under optimal conditions rarely retains viability beyond 3 weeks in antibiotic-nutrient solution and is severely impaired after 2 weeks. This casts serious doubts on viability in current clinical homograft valve practice.
D000818:Animals; D000900:Anti-Bacterial Agents; D001021:Aortic Valve / Q000378:metabolism; D003094:Collagen / Q000096:biosynthesis*; D046508:Culture Techniques; D004285:Dogs; D005347:Fibroblasts / Q000254:growth & development; D006351:Heart Valves / Q000378:metabolism / Q000637:transplantation*; D014176:Protein Biosynthesis; D011664:Pulmonary Valve / Q000378:metabolism; D013242:Sterilization / Q000379:methods*; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014022:Tissue Survival
D000900:Anti-Bacterial Agents; D003094:Collagen