Activation of platelets by platelet activating factor (PAF) derived from IgE-sensitized basophils. IV. PAF does not activate platelet factor 3 (PF3).
Platelet activating factor (PAF) derived from antigen-stimulated, IgE-sensitized rabbit basophils acts on platelets to induce aggregation and secretion of their content of granule-bound vasoactive amines. Despite this, PAF did not activate platelet factor 3. In contrast, collagen induced aggregation, secretion and PF3 activation in the washed platelets. Other stimuli (ADP, C3b, thrombin) also initiated both secretion and PF3 activation. A wide dose range of PAC, including those giving maximal secretion and aggregation, were ineffictive in making PF3 available and the possibility that PAF inhibited PF3, or its generation, was also excluded. It is concluded that PAF is a unique stimulus for platelets and that secretion and aggregation are not necessarily accompanied by PF3 generation.
D000818:Animals; D001491:Basophils / Q000378:metabolism*; D001779:Blood Coagulation Factors / Q000096:biosynthesis* / Q000378:metabolism; D001780:Blood Coagulation Tests; D003094:Collagen / Q000494:pharmacology; D005260:Female; D007073:Immunoglobulin E / Q000378:metabolism*; D008297:Male; D010974:Platelet Aggregation; D010977:Platelet Factor 3 / Q000096:biosynthesis*; D011817:Rabbits; D012701:Serotonin / Q000378:metabolism; D012910:Snake Venoms / Q000494:pharmacology
D001779:Blood Coagulation Factors; D012910:Snake Venoms; D012701:Serotonin; D010977:Platelet Factor 3; D007073:Immunoglobulin E; D003094:Collagen