Preparation of monovalent succinyl-concanavalin A and its mitogenic activity.
Monovalent succinyl-concanavalin A was prepared by photoaffinity labeling of succinyl-concanavalin A with p-azidophenyl a-D-mannopyranoside. This concanavalin A derivate showed a weak but definite mitogenic acitivity against mouse splenic lymphocytes and human peripheral lymphocytes, suggesting that direct receptor cross-linkage on the cell surface is not an obligatory prerequisite for the activation of lymphocytes.
D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D003208:Concanavalin A / Q000494:pharmacology; D006386:Hemagglutination Tests; D008213:Lymphocyte Activation / Q000187:drug effects; D008722:Methods; D051379:Mice; D008934:Mitogens; D011485:Protein Binding; D013329:Structure-Activity Relationship; D013386:Succinates
D000345:Affinity Labels; D008934:Mitogens; D013386:Succinates; D003208:Concanavalin A