Autoreactivity developing spontaneously in cultured mouse spleen cells. I. Evidence that cytotoxicity is directed against embryo-associated antigen.
We have investigated the ability of male-mouse spleen cells before and after culture in the absence of deliberate antigenic stimulation to show specific cytotoxicity to syngeneic embryo-fibroblast cells. The data suggest that cytotoxicity which develops spontaneously in such spleen cell cultures is directed primarily against embryo-associated antigens. Syngeneic Con-A-stimulated spleen cells, which, unlike fresh normal spleen cells, are also lysed by rabbit anti-mouse embryo antisera, are also a suitable target to demonstrate spontaneously developing cytotoxicity.
D000818:Animals; D000941:Antigens; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D004306:Dose-Response Relationship, Immunologic; D004622:Embryo, Mammalian / Q000276:immunology*; D005260:Female; D005347:Fibroblasts / Q000276:immunology; D006648:Histocompatibility; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology*
D000941:Antigens