A new biological assay method for histamine-sensitizing factor using survival time as a response.
A new biological assay method using survival time of mice as a graded response after histamine challenge has been developed for the histamine-sensitizing factor of pertussis bacilli. The method is relatively precise and reproducible in estimation and fairly sensitive in the validity tests of biological assay. Effect of endotoxin on the estimation by the proposed method was also investigated in case the method is applied to the control test of pertussis vaccine.
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D004731:Endotoxins / Q000494:pharmacology*; D006632:Histamine / Q000276:immunology*; D051379:Mice; D010567:Pertussis Vaccine; D013997:Time Factors
D004731:Endotoxins; D010567:Pertussis Vaccine; D006632:Histamine