Coenzyme-A-dependent esterification of cholesterol in rat intestinal mucosa.
Rat intestinal mucosa contains an enzyme which catalyses the esterification of cholesterol. The enzyme is CoA-dependent and probably an acyl-CoA: cholesterol acyltransferase (ACAT) (E.C.2.3.1.26). Maximal activity in vitro is obtained when long chain acylcarnitines, CoA and carnitine palmityltransferase are used as an acyl-CoA generating system. The enzyme is localized in the microsomal fraction, has a pH optimum between 6.4-7.2, and oleate is the preferred acyl group. The activity of the enzyme is highest in the proximal jejunum, and has a capacity that can account for all cholesteryl esters found in intestinal rat lymph.
D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D002333:Carnitine Acyltransferases / Q000378:metabolism; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D003065:Coenzyme A / Q000031:analogs & derivatives*; D004951:Esterification; D007413:Intestinal Mucosa / Q000201:enzymology / Q000378:metabolism*; D007421:Intestine, Small / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D002785:Sterol O-Acyltransferase / Q000378:metabolism
D000105:Acetyl Coenzyme A; D002784:Cholesterol; D002333:Carnitine Acyltransferases; D002334:Carnitine O-Palmitoyltransferase; D002785:Sterol O-Acyltransferase; D003065:Coenzyme A