Reactivity in tissue tryptophyl units in histofluorescence methods using carbonyl compounds.
The contribution of tissue tryptophyl residues, with both amino and carboxyl groups linked to the peptide bonds, to visible fluorescence was studied following various histochemical methods. Tryptophan residues of chymotrypsinogen and trypsinogen exhibited visible fluorescence after (1) combined formaldehyde-HCl vapour, (2) combined formaldehyde and acetyl chloride vapour, and (3) glyoxylic acid vapour treatment.
D000085:Acetates; D000447:Aldehydes; D000818:Animals; D005557:Formaldehyde; D006038:Glyoxylates; D006651:Histocytochemistry; D006851:Hydrochloric Acid; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D051381:Rats; D014364:Tryptophan / Q000032:analysis*
D000085:Acetates; D000447:Aldehydes; D006038:Glyoxylates; D005557:Formaldehyde; D014364:Tryptophan; D006851:Hydrochloric Acid