Quantitative determination of hexosamines in glycoprotein by ion-exchange chromatography.
An ion-exchange chromatographic method for the quantitative determination of glucosamine and galactosamine in glycoproteins is described. The hexosamines are completely separated from interfering peptides of the acid hydrolysate using a small column of cation exchanger in a manner described earlier by Boas. Chromatographic separation of the hexosamine fraction and the determination of glucosamine and galactosamine are accomplished on an amino acid analyzer with ninhydrin.
D002852:Chromatography, Ion Exchange; D005688:Galactosamine / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006595:Hexosamines / Q000032:analysis*
D006023:Glycoproteins; D006595:Hexosamines; D005688:Galactosamine