Effect of endogenous corticosterone on the determination of dexamethasone receptor levels in rat liver cytosol.
The effect of endogenous corticosterone on the quantitative measurement of dexamethasone receptors in liver cytosols from developing rats has been studied. Liver cytosols from adrenalectomized rats were preincubated with increasing concentrations of nonlabeled corticosterone and the levels of detectable dexamethasone receptors were subsequently determined either directly or after removal of unbound corticosterone. Corticosterone concentrations of 50 nM or lower had no significant effect on the specific binding of labeled dexamethasone. Higher concentrations of corticosterone resulted in under-estimation of dexamethasone receptor levels. The mean levels of endogenous corticosterone in liver cytosols from 19.5- to 21.5- day fetuses, 22-day fetuses, 6-day-old immature rats and adult rats were 27.40, 11.91, 0.81 and 4.05 nM, respectively. It is concluded that variations in the levels of circulating corticosterone in the rat under normal physiological conditions have no significant effect on the quantitative measurement of total (occupied and unoccupied) receptor sites for dexamethasone in liver cytosol. This is supported by the finding that prior treatment of liver cytosols, from rats at different stages of development, with charcoal to remove unbound steroids has no effect on the amount of detectable dexamethasone receptors.
D000818:Animals; D001665:Binding Sites; D003345:Corticosterone / Q000032:analysis / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism*; D005938:Glucocorticoids / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D011485:Protein Binding; D011863:Radioimmunoassay; D051381:Rats; D011955:Receptors, Drug / Q000187:drug effects*; D013696:Temperature
D005938:Glucocorticoids; D011955:Receptors, Drug; D003907:Dexamethasone; D003345:Corticosterone