title: Further studies on collagen mRNA: partial chemical characterization and polyadenylic acid sequence.
abstract: Collagen mRNA has already been purified and characterized by us. Its purity has now been enhanced by two different methods. Gel electrophoresis shows in either method, a single peak with the same mobility already reported: 1.05 X 10(6) daltons. Base composition analyses of collagen mRNA purified by either method were almost identical. Chemical analyses of the isolated polyadenylic acid stretch show that it is, 0.48 X 10(5) daltons-long, (about 140 nucleotides-long), contains 75% AMP, and is located at the 3' end of the polymer.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002846:Chromatography, Affinity; D003094:Collagen / Q000096:biosynthesis*; D004586:Electrophoresis; D005260:Female; D009865:Oocytes / Q000378:metabolism; D011061:Poly A / Q000032:analysis*; D012333:RNA, Messenger / Q000378:metabolism; D014981:Xenopus
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D003094:Collagen