A comparative study of micro-ammonia determinations in plasma using two different methods.
Two commercially available kits for determining blood ammonia were compared, an enzymatic method requiring a sample volume of 100mul and a nonenzymatic method requiring 1 ml of sample. Calibration curves for both kits were linear to 270 mumol/l, however correlation studies on plasma samples revealed a lack of agreement between methods (r equal to 0.825, Y equal to 0.76X +51). Improved correlation occurs when protein interference is eliminated from the enzymatic procedure. Finally, the nonenzymatic method is modified to accommodate 100 mul of sample and compared to the original method.
D000641:Ammonia / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D007202:Indicators and Reagents; D008832:Microchemistry; D013053:Spectrophotometry / Q000379:methods
D007202:Indicators and Reagents; D000641:Ammonia