Membrane Ig on MPC11 myeloma cells: correlation between the expression of membrane Ig, a receptor for Ig and the process of secretion.
The expression of membrane immunoglobulin (mIg) was examined in three cloned MPC11-derived mouse myeloma cell lines. Membrane immunofluorescence studies demonstrated that IgG2b producer cells (P1) had complete IgG molecules, L-chain producer (L1) had only L-chain determinants and nonproducer (NP2) did not have any Ig determinants on the cell surface. An Ig receptor, with characteristics different from B lymphocyte Fc receptor, has been found to be present on secreting cells (P1 or L1), but not on the NP2 cell variant. The data reported in the present paper indicate that the expression of mIg and of the Ig receptor molecule is clearly correlated with the process of secretion. In the light of previous data reported on Ig secretion, a model is proposed which correlates the process of secretion with the expression not only of mIg, but also of the receptor for Ig.
D001666:Binding Sites, Antibody; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D007074:Immunoglobulin G / Q000378:metabolism*; D007136:Immunoglobulins / Q000378:metabolism; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology; D009194:Myeloma Proteins / Q000378:metabolism*; D011402:Pronase; D011485:Protein Binding; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*
D007074:Immunoglobulin G; D007136:Immunoglobulins; D009194:Myeloma Proteins; D011947:Receptors, Antigen, B-Cell; D003513:Cycloheximide; D011402:Pronase