Radioimmunoassay of prostaglandins E1, E2, and F2alpha in unextracted plasma, serum and myocardium.
A radioimmunoassay procedure for the determination of PGE1, PGE2, and PGF2alpha is presented. The procedure involves the pre-precipitation of each prostaglandin specific antiserum with the precipitating antisera (ARGG), and the use of these antisera mixtures in assaying for PGE1, PGE2, and PGF2alpha. Applicability of the methods to unextracted plasma, serum and myocardial homogenate has been demonstrated through tests of specificity, recovery, reproducability and parallelism. A mathematical correction for cross-reactivity between PGE1 and PGE2, and their opposing antisera is given. To demonstrate the utility of the methodology in differentiation of experimental variables, prostaglandin concentrations in unincubated serum, incubated serum, and the rate of prostaglandin production in serum of dogs are given.
D000818:Animals; D001666:Binding Sites, Antibody; D004285:Dogs; D005590:Fractional Precipitation; D007106:Immune Sera; D007202:Indicators and Reagents; D009206:Myocardium / Q000032:analysis*; D011458:Prostaglandins E / Q000032:analysis* / Q000096:biosynthesis / Q000097:blood; D011460:Prostaglandins F / Q000032:analysis* / Q000096:biosynthesis / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods*
D007106:Immune Sera; D007202:Indicators and Reagents; D011458:Prostaglandins E; D011460:Prostaglandins F