Comparison of procedures for extracting free amino acids from polymorphonuclear lekocytes.
We studies five methods for extracting amino acids from human polymorphonuclear leukocytes. Both the use of cell lysis and of a deproteinizing agent interfere with quantitative determination of the amino acids, basic amino acids being the most sensitive to the extraction procedure. Among the methods used, disruption of the cells by freezing-thawing is the best method for extracting all the amino acids. Taurine is the only amino acid extracted in the same amount by all the methods studied, and it represents half of the intracellular pool.
D000328:Adult; D000596:Amino Acids / Q000097:blood* / Q000302:isolation & purification; D006098:Granulocytes / Q000032:analysis*; D006801:Humans; D007962:Leukocytes / Q000032:analysis*; D008297:Male; D008722:Methods; D009504:Neutrophils / Q000032:analysis*; D012997:Solvents
D000596:Amino Acids; D012997:Solvents