title: Effect of alveolar lining material on phagocytic and bactericidal activity of lung macrophages against Staphylococcus aureau.
abstract: Unstimulated rabbits were sacrificed and their lungs washed with heparinized saline. After alveolar macrophages were harvested, the cell-free lavage fluid was centrifuged at 47,000 X g to recover a small, whitish, surface-active pellet (F fraction.) The supernatant was concentrated 15-fold by vacuum dialysis (P fraction). Alveolar macrophages in a serum-free system were challenged with radiolabeled (32P) Staphylococcus aureus preincubated in either balanced salt solution or F or P fraction. A small increase in alveolar macrophage bacterial uptake occurred with P fraction-treated staphylococci. P fraction from locally immunized animals further enhanced phagocytosis. In bactericidial experiments, alveolar macrophages were allowed to phagocytize staphylococci preincubated in either balanced salt solution or F fraction. Intracellular bactericidal activity of alveolar macrophages was quantitated by lysotaphin lysis of extracellular bacteria and quantitation of viable intracellular bacteria. Enhanced lung macrophage bactericidal activity against F fraction-incubated staphylococci was noted.
mesh_terms: D000336:Aerosols; D000373:Agglutinins / Q000032:analysis; D000818:Animals; D001980:Bronchi / Q000276:immunology; D007114:Immunization; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D066298:In Vitro Techniques; D008168:Lung / Q000032:analysis / Q000382:microbiology / Q000502:physiology*; D008264:Macrophages / Q000382:microbiology / Q000502:physiology*; D010587:Phagocytosis; D011650:Pulmonary Alveoli / Q000166:cytology / Q000382:microbiology / Q000502:physiology*; D011817:Rabbits; D013211:Staphylococcus aureus
chemical_list: D000336:Aerosols; D000373:Agglutinins; D007070:Immunoglobulin A; D007074:Immunoglobulin G