Erythroid colony formation (CFUe) in fetal liver and adult bone marrow and spleen from the mouse.
The methyl cellulose modification of the CFUe technique has been applied to 14 day fetal liver and adult bone marrow and spleen from CBA/CA mice. Optimized doses of fetal calf serum, alpha-thioglycerol, erythropoietin and cell suspensions have been obtained from dose response curves in order to standardize the technique. The slopes of the erythropoietin and cell dose response curves indicate a greater sensitivity by fetal liver to the hormone than bone marrow or spleen. The proportion of cells in the DNA synthesis phase of the cell cycle, as measured by the CFUe technique, has been estimated by administering hydroxyurea. Two hours after the drug was injected, 89% of fetal liver cells, 71% of bone marrow cells and 81% of spleen cells were found to be in the S-phase.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004920:Erythropoiesis / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D005990:Glycerol / Q000031:analogs & derivatives / Q000494:pharmacology; D006918:Hydroxyurea / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D008747:Methylcellulose / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D011247:Pregnancy; D013154:Spleen / Q000187:drug effects
D004921:Erythropoietin; D008747:Methylcellulose; D005990:Glycerol; D006918:Hydroxyurea