title: Separation of 5'-mono-, di- and triphosphates of 1-beta-D-arabinofuranosylcytosine and of 1-beta-D-arabinofuranosyluracil by thin-layer and high pressure liquid chromatography.
abstract: The separation of all the 5'-mono-, di- and triphosphates of ara-C and ara-U by thin-layer (TLC) and high pressure liquid chromatography (HPLC) is described. Whereas for the TLC two different systems are necessary, HPLC allows a rapid separation of all the nucleotides with high resolution, reproducibility, and sensitivity within 15 minutes. An example of an HPLC separation of ara-C and ara-U nucleotides derived from a biological sample is presented.
mesh_terms: D001086:Arabinofuranosyluracil / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003561:Cytarabine / Q000031:analogs & derivatives* / Q000032:analysis; D003597:Cytosine Nucleotides / Q000032:analysis*; D011741:Pyrimidine Nucleosides / Q000031:analogs & derivatives*; D013997:Time Factors; D014500:Uracil Nucleotides / Q000032:analysis*
chemical_list: D003597:Cytosine Nucleotides; D011741:Pyrimidine Nucleosides; D014500:Uracil Nucleotides; D003561:Cytarabine; D001086:Arabinofuranosyluracil