The absence of histone H1 from the chromatin fraction obtained by sonication of calf thymus nuclei under "quasiphysiological" ionic conditions.
The minor chromatin fraction was isolated from the sonicated calf thymus nuclei on the basis of its differential solubility in the "quasiphysiological" salt medium (0.1 M KCl-0.05 M NaCl-l mM MgCl2-1 mM CaCl2). Histone Hl is almost completely absent from this fraction. DNA isolated from this fraction occurs in three discrete low mol. wt. fragments. The fraction of chromatin which lacks histone Hl can also be obtained by two other methods. On of them consists in salt precipitation of the chromatin gel and its subsequent sonication. The second method includes precipitation of the sonicated chromatin gel by salts. In the first case the properties of the chromatin fraction which remains in the supernatant after centrifugation closely resemble those of the original salt-soluble nuclear fraction. The second method yields supernatant fraction also lacking histone Hl but containing heterogeneous DNA. Comparisons were also made of the sonically-solubilized nuclear fractions obtained in the complete salt medium and its mono and divalent cationic constituents.
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis*; D002843:Chromatin / Q000032:analysis*; D003852:Deoxyribonucleoproteins / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis*; D008970:Molecular Weight; D009994:Osmolar Concentration; D012260:Ribonucleases; D013010:Sonication; D013950:Thymus Gland / Q000032:analysis
D002843:Chromatin; D003852:Deoxyribonucleoproteins; D006657:Histones; D012260:Ribonucleases