J blood group active lipoproteins extracted from bovine erythrocytes.
Ghosts of J-positive bovine erythrocytes were frozen overnight, thawed the next day, washed with ion-free water, and then extracted with a water-n-pentanol mixture. After centrifugation, the aqueous phase contained a great deal of the membrane proteins and lipids in a soluble form along with the J blood-group activity. After preparative ultracentrifugation, about 2/3 of the J activity were recovered in the high-density lipoprotein fraction, while the low-density lipoprotein fraction contained 1/3 J activity. This result is consistent with out finding that on treatment of the stroma lipoproteins with dextran sulphate, about 2/3 of J activity were recovered in the supernatant, 1/3 in the precipitate. These fractions obtained by dextran sulphate treatment were characterized by protein and lipid assay.
D000818:Animals; D000941:Antigens; D001789:Blood Group Antigens; D002417:Cattle / Q000097:blood*; D004912:Erythrocytes / Q000276:immunology*; D008074:Lipoproteins / Q000302:isolation & purification*; D014461:Ultracentrifugation
D000941:Antigens; D001789:Blood Group Antigens; D008074:Lipoproteins