Separation of permethylated isomeric glucuronides by gas chromatography and analysis of the mass spectra.
Glucuronic acid conjugates of several foreign compounds (1- and 2-naphthols, 2-, 3-, and 4-hydroxybiphenyls, m- and p-hydroxyphenylphenylhydantoins) were produced in the isolated perfused rat liver and identified in the bile as the permethyl derivatives. Permethylated glucuronide isomers were easily separated by gas chromatography on SE-30 and OV-17 columns. The mass spectra of permethylated glucuronide isomers were very similar. Gas chromatography proved to be most useful for the separation and identification of isomeric glucuronides.
D000818:Animals; D001646:Bile / Q000032:analysis; D001713:Biphenyl Compounds / Q000032:analysis; D002849:Chromatography, Gas; D005965:Glucuronates / Q000032:analysis*; D007536:Isomerism; D008099:Liver / Q000032:analysis; D013058:Mass Spectrometry; D008722:Methods; D008745:Methylation; D009284:Naphthols / Q000032:analysis; D010672:Phenytoin / Q000032:analysis; D051381:Rats
D001713:Biphenyl Compounds; D005965:Glucuronates; D009284:Naphthols; D010672:Phenytoin