Cellular monosaccharide patterns of Neisseriaceae.
Sixty-four strains of Neisseria, Moraxella, and Acinetobacter were screened for cellular monosaccharides by gas-liquid chromatography and other chromatographic techniques. The four sugars ribose, glucose, glucosamine, and 2-keto-3-deoxyoctonate (KDO) were detected in all strains. Heptose was detected only in "true neisseriae" (Neisseria gonorrhoeae, N. meningitidis, N. sicca, N. cinerea, N. flavescens, and N. elongata) and in the tentaively named species Moraxella urethralis. Some marked interspecies dissimilarities within groups were revealed. Thus, N. ovis and M. atlantae were characterized by the presence of mannose. Intraspecies differences were also encountered. N. meningitidis strains of serogroups B and C were distinguished from strains of serogroup A by their sialic acid content. This sugar was also detected in two out of three examined strains of M. nonliquefaciens. In Acinetobacter, heterogeneity of monosaccharide patterns was rather pronounced. The results show the applicability of gas chromatographic "monosaccharide" profiles fo whole cells or extracted carbohydrate in bacterial classification and identification, including differentiation at the subspecies level. In addition, such profiles may be useful for monitoring during purification of cellular polysaccharides.
D000150:Acinetobacter / Q000032:analysis*; D002849:Chromatography, Gas; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006539:Heptoses / Q000032:analysis; D007661:Ketoses / Q000032:analysis; D008358:Mannose / Q000032:analysis; D009005:Monosaccharides / Q000032:analysis*; D009016:Moraxella / Q000032:analysis*; D009343:Neisseria / Q000032:analysis*; D012210:Rhamnose / Q000032:analysis; D012266:Ribose / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis; D013400:Sugar Acids / Q000032:analysis
D006539:Heptoses; D007661:Ketoses; D009005:Monosaccharides; D012794:Sialic Acids; D013400:Sugar Acids; D012266:Ribose; D005947:Glucose; D005944:Glucosamine; D008358:Mannose; D012210:Rhamnose; D005690:Galactose