[Several methodologic features of prolonged human lymphocyte storage].
When using in the lymphocytotoxic reaction lymphocytes stored in frozen condition the proportion of perished cells after thawing should not exceed 10-20%. Therefore, a study was made of the influence of various concentrations of dimethylsulfoxide (DMSO), composition of the medium and of other parameters for the purpose of elaboration of the optimal conditions of freezing human lymphocytes. The best results were obtained in freezing cells in the autologous serum with 12.5% DMSO. However sufficiently favourable results were also seen in using AB serum and 20% bovine serum in Eagle's medium.
D001793:Blood Preservation / Q000379:methods*; D004121:Dimethyl Sulfoxide; D005615:Freezing; D006801:Humans; D007106:Immune Sera; D008214:Lymphocytes
D007106:Immune Sera; D004121:Dimethyl Sulfoxide
Nekotorye metodicheskie osobennosti dlitel'nogo khraneniia limfotsitov cheloveka