Glycerolpropylsilane bonded phases in the steric exclusion chromatography of biological macromolecules.
Glycerolpropylsilane bonded phases have been found to control the adsorption and/or denaturation of proteins and nucleic acids on controlled porosity glass supports. The bonded-phase thickness is 18-19A while the amount of glycerol moiety varies from 80 to 150 mumoles/g depending on support pore diameter. It has been demonstrated that carbohydrate bonded supports may be used in the chromatography of proteins, nucleic acids, and polysaccharides.
D000327:Adsorption; D002850:Chromatography, Gel; D005990:Glycerol / Q000031:analogs & derivatives; D006801:Humans; D009691:Nucleic Acid Denaturation; D009696:Nucleic Acids / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*; D011489:Protein Denaturation; D011506:Proteins / Q000032:analysis*; D012821:Silanes; D012825:Silicon
D009696:Nucleic Acids; D011134:Polysaccharides; D011506:Proteins; D012821:Silanes; D005990:Glycerol; D012825:Silicon