Gardimycin, a new antibiotic from Actinoplanes. II. Isolation and preliminary characterization.
The strain Actinoplanes garbadinensis nov. sp. produces a peptide antibiotic, named gardimycin, which is active in vitro and in vivo against Gram-positive bacteria. Isolation and purification of the product have been accomplished by extraction from the broth with butanol and dialysis of the crude extract, followed by counter-current distribution. Gardimycin is an open chain peptide with an approximate minimal formula C84H138N18S3-4O34Na. The following amino acids have been identified by column chromatography of an acid hydrolysate: serine, glutamic acid, alanine, leucine, isoleucine, glycine, valine and two sulphur-containing amino acids whose structure is presently under study. Tryptophan has been identified in an alkaline hydrolysate.
D000192:Actinomycetales; D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003460:Crystallization; D003470:Culture Media / Q000032:analysis; D006868:Hydrolysis
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D003470:Culture Media