The presence of aromatic L-amino acid decarboxylase in certain intestinal nerve cells.
The presence of aromatic 1-amino acid decarboxylase (AADC) in nerve cell bodies of the intrinsic plexuses of the guinea-pig small intestine was demonstrated by incubating segments of intestine with 1-dopa in the presence of an inhibitor of monoamine oxidase, pargyline. After such incubation, some nerve cell bodies gave a fluorescence histochemical reaction indicative of the presence of a decarboxylated product of 1-dopa, probably dopamine. No fluorescence reaction occurred in the unincubated control or if the inhibitor of AADC, RO 4-4602, was included in the incubation mixture. The AADC-containing cell bodies apparently do not take up and store dopamine, because no fluorescence could be detected after incubation with dopamine and a monoamine oxidase inhibitor. The AADC-containing cells were found in about half of the ganglia of the submucous plexus of the guinea-pig small intestine, but were considerably less frequent in the myenteric plexus. They were also found in the other areas examined in this study, that is, in both enteric plexuses of the guinea-pig distal colon and of the small intestines of rabbits and rats.
D000818:Animals; D001545:Benserazide / Q000494:pharmacology; D004296:Dopa Decarboxylase / Q000032:analysis*; D004298:Dopamine / Q000378:metabolism; D006168:Guinea Pigs; D008856:Microscopy, Fluorescence; D009197:Myenteric Plexus / Q000201:enzymology*; D011817:Rabbits; D051381:Rats; D013368:Submucous Plexus / Q000201:enzymology*
D001545:Benserazide; D004296:Dopa Decarboxylase; D004298:Dopamine