Rapid procedure for assessment of compounds that modify uptake and release of tritiated norepinephrine.
A rapid procedure to estimate tritiated norepinephrine (levarterenol) in a single mouse heart is described. The method is based upon oxidation of the tritium in the tissue to tritiated water, which is then determined by liquid scintillation spectroscopy. Large numbers of samples can be assayed with great facility. The effects of standard compounds that modify the uptake and release of 3H-norepinephrine in heart tissue were determined with this system, and a procedure for studying their interactions is described.
D000818:Animals; D004353:Drug Evaluation, Preclinical; D004347:Drug Interactions; D066298:In Vitro Techniques; D008722:Methods; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D009638:Norepinephrine