title: Plasminogen activator in early embryogenesis: enzyme production by trophoblast and parietal endoderm.
abstract: We have surveyed the early stages in the development and differentiation of cultured mouse embryos for plasminogen activator production. This enzyme is first detectable by the sixth equivalent gestation day. Thereafter, cultured blastocysts produce plasminogen activator with a biphasic time course: in the first phase, enzyme secretion rises to a maximum at about the eighth day and then decreases; a second phase, during which more enzyme accumulates, begins somewhat later and continues to at least the fifteenth day. By fractionating the blastocyst into its constituent cell types, we have identified the trophoblast as the cells responsible for the first phase of enzyme synthesis. The pattern of enzyme production by the trophoblast is closely correlated with the invasive period of these cells in vivo and implies that plasminogen activator is involved in embryo implantation. The second phase of plasminogen activator production is due to parietal endoderm, which initiates enzyme synthesis upon differentiation from the inner cell mass. The properties of the parietal endoderm suggest that plasminogen activator may participate in the migration of these cells and/or in the metabolism of Reichert's membrane which accompanies embryo growth. These results are consistent with the concept, deveolped from work on other cell types, that plasminogen activator may represent a generalized mechanism for tissue remodeling and cell migration.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000201:enzymology; D002454:Cell Differentiation; D002465:Cell Movement; D004707:Endoderm / Q000201:enzymology*; D007700:Kinetics; D051379:Mice; D010960:Plasminogen Activators / Q000096:biosynthesis* / Q000502:physiology; D013997:Time Factors; D014327:Trophoblasts / Q000201:enzymology*
chemical_list: D010960:Plasminogen Activators