Induction of type B virions to bud into cytoplasmic vacuoles in a mammary tumor of an X-ray- and urethan-treated X/Gf mouse.
Electron microscopic studies of thin sections of a mammary carcinoma that developed in a female mouse of the syngeneic X/Gf low-cancer strain after treatments with X-rays (3 X 100 rads weekly) followed by 8 weekly i.p. injections of urethan (1 mg/g body weight) revealed extensive budding of B particles into cytoplasmic vacuoles and numerous characteristic B virions in the vacuolar lumens. Simultaneous budding of B particles into cytoplasmic vacuoles and budding of B particles at plasma membranes of the same cancer cell were also noted (figs. 2 to 6).
D000230:Adenocarcinoma / Q000382:microbiology* / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000382:microbiology / Q000648:ultrastructure; D003593:Cytoplasm / Q000382:microbiology* / Q000648:ultrastructure; D008325:Mammary Neoplasms, Experimental / Q000382:microbiology* / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009940:Organoids / Q000382:microbiology*; D014520:Urethane; D014617:Vacuoles / Q000382:microbiology* / Q000648:ultrastructure; D014780:Viruses / Q000254:growth & development*; D014965:X-Rays
D014520:Urethane