More sensitive high-pressure liquid-chromatographic determiantion of theophylline in serum.
We present procedures for determining theophylline in 50 mul of serum. The drug is extracted into a small volume of solvent that contains an internal standard, 8-chlorotheophylline. The extract is Analyzed by isocratic reversedphase chromatography, with measurement of eluted theophylline at 273 nm. Day-to-day reproducibility within 5% is attainable for the concentration range 5--20 mg/liter. Other xanthines and related metabolites do not interfere. Sensitivity is 1 mg/liter. The correlation coefficient, when results by a spectrophotometric procedure were compared, was 0.989. Amobarbital, secobarbital, phenobarbital, and diphenylhydantoin do not interfere. Total analysis time for a single sample is 15 min.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008832:Microchemistry; D012044:Regression Analysis; D013053:Spectrophotometry / Q000379:methods; D013806:Theophylline / Q000097:blood*
D013806:Theophylline