[Separation of thiamine phosphoric esters on cation-exchange Saphadex].
A new method is suggested for separation of thiamine phosphoric esters (TPE) from biological materials. The aliquote of evaporated extract from tissue was applied to the column with Sephadex SE-C-25 (25X2.5), TTP passed through Sephadex with 0,005 M acetate buffer, pH 3.8-4.0, TDP was eluated with 0,05 M acetate buffer; TMP and T--1 M NaC1 in 1.0 M acetate buffer. Yield of each phosphoric ester was about 100%. The method allows separating thiamine phosphoric esters without preliminary purification of charcoal. It may be preferable to determine the content of TTP in tissue.
D000818:Animals; D002021:Buffers; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D007475:Ion Exchange Resins; D008099:Liver / Q000032:analysis; D010755:Organophosphates / Q000302:isolation & purification*; D009943:Organophosphorus Compounds / Q000302:isolation & purification*; D051381:Rats; D013831:Thiamine / Q000302:isolation & purification*
D002021:Buffers; D007475:Ion Exchange Resins; D010755:Organophosphates; D009943:Organophosphorus Compounds; D013831:Thiamine
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