Enzymes of 2-oxo acid degradation and biosynthesis in cell-free extracts of mixed rumen micro-organisms.
The enzymes of 2-oxo acid decarboxylation and 2-oxo acid synthesis (EC 1.2.7.1 and EC 1.2.7.2) were isolated and partially purified from cell-free extracts of rumen micro-organisms. The lyase was active with pyruvate, 3-hydroxypyruvate and 2-oxobutyrate. The synthase was active with acetate, 2-oxoglutarate or succinate. Pyruvate synthase was separated from pyruvate lyase by Sephadex G-200 gel filtration. With Sephadex filtration, approximate mol.wts. of 310000 and 210000 were determined for pyruvate lyase and pyruvate synthase respectively.
D000093:Acetoin; D000445:Aldehyde Oxidoreductases / Q000302:isolation & purification*; D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D002087:Butyrates; D002104:Cadmium / Q000494:pharmacology; D002474:Cell-Free System; D003065:Coenzyme A; D004229:Dithiothreitol / Q000494:pharmacology; D004589:Electrophoresis, Disc; D005288:Ferredoxins; D005182:Flavin-Adenine Dinucleotide; D005978:Glutathione / Q000494:pharmacology; D007656:Ketoglutaric Acids; D008190:Lyases / Q000302:isolation & purification; D011422:Propionates; D011773:Pyruvates; D012417:Rumen / Q000201:enzymology / Q000382:microbiology*
D002087:Butyrates; D005288:Ferredoxins; D007656:Ketoglutaric Acids; D011422:Propionates; D011773:Pyruvates; D002104:Cadmium; D005182:Flavin-Adenine Dinucleotide; D000093:Acetoin; D000445:Aldehyde Oxidoreductases; D008190:Lyases; D005978:Glutathione; D001151:Arsenic; D003065:Coenzyme A; D004229:Dithiothreitol