[Stability of physico-chemical parameters of DNA during long-term storage].
DNA was extracted from calf thymus and sturgeon milt and purified of admixtures; this highly polymeric DNA was studied under conditions of its prolonged storage (for 1 1/2-2 years) at a temperature of 0-4 degrees C in 1 M NaCl in the form of a precipitate under a 70% ethanol and in the lyophilized state. It appeared that DNA obtained from the mentioned sources could be stored for at least 1 1/2 years under the said conditions without any significant damage of its polymeric properties or secondary structure. Although DNA from sturgeon milt had the initial physico-chemical indices similar to those of the thymus DNA, the former displayed much greater stability under the same storage condition, indicating stability of its molecular structure.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D005399:Fishes; D013950:Thymus Gland / Q000032:analysis; D013997:Time Factors
D004247:DNA
Issledovanie stabil'nosti fiziko-khimicheskikh parametrov DNK pri dlitel'nom khranenii