Induction of nephrotoxic nephritis with purified antibody: preparation, properties and assay of antibody.
Antibody to rabbit glomerular basement membrane was separated from whole antiserum by adsorption onto and elution from rabbit glomeruli in vitro. The physico-chemical and biological properties were investigated. The preparation had a high specific activity for the GBM and induced glomerulonephritis on injection into rabbits. About 10% of the antibody localised in the kidneys of rabbits and the total in vitro and in vivo tissue binding capacity were comparable. Based on these results a simple in vitro assay for measuring anti-GBM antibody is described.
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification*; D000918:Antibody Specificity; D003165:Complement System Proteins / Q000378:metabolism; D005921:Glomerulonephritis / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis; D007678:Kidney Glomerulus / Q000276:immunology; D009928:Organ Specificity; D011817:Rabbits; D014461:Ultracentrifugation
D000906:Antibodies; D007074:Immunoglobulin G; D003165:Complement System Proteins