Amino acid metabolism of myeloma cells in culture.
The growth of myeloma cells in Leibovitz medium supplemented with 20% serum was limited by the depletion of glutamine. A simple modification of the Leibovitz medium by increasing the concentrations of glutamine, lysine, isoleucine, leucine, sodium pyruvate, galactose, and vitamins resulted in over 100% increase in cell growth yield. The total myeloma protein produced by the cells was increased by approximately 90% in modified Leibovitz media. Analysis of spent culture media for 19 amino acids showed that the concentrations of 8 amino acids were reduced; those of 5 amino acids were increased and the other 6 did not change significantly.
D000596:Amino Acids / Q000378:metabolism*; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D005973:Glutamine / Q000378:metabolism; D007074:Immunoglobulin G / Q000096:biosynthesis; D007532:Isoleucine / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism
D000596:Amino Acids; D003470:Culture Media; D007074:Immunoglobulin G; D007532:Isoleucine; D005973:Glutamine; D008715:Methionine; D008239:Lysine