Activation of the alternative complement pathway by water-insoluble glucans of streptococcus mutans: the relation between their chemical structures and activating potencies.
Water insoluble, sticky glucan was synthesized by reacting glucosyltransferase obtained from the sulture filtrate of cariogenic Streptococcus mutans OMZ 176 on sucrose. This glucan (OMZ 176 glucan), that consisted of the backbone chains of consecutive alpha-1, 3 glucosidic linkages (65%) and the side chain of alpha-1, 6 glucosidic bonds (35%), showed an ability to activate the alternative pathway of the complment system in human serum. Water soluble glucans synthesized from the reaction of glycosyltransferases of S. mutans strain AHT, FA-I, and Ingbritt with sucrose, that have a high content of alpha-1,6 and a low content of alpha-1, 3 glucosidic linkages, were less effective to activate the complement system. Sephadex (G-25) and Dextran (T-2,000 and T-40), that consisted exclusively of alpha-1, 6 glucosidic linkages, did not significantly activate the complement system. Controlled Smith degradation products of OMZ 176 glucan, composed of the alpha-1, 3 linked glucose backbone chain alone, lost its activity. From these results it was concluded that both backbone chain of alpha-1, 3 glucose units and branched alpha-1, 6 glucose units were essential for the OMZ 176 glucan to activate the alternative pathway of the human complement system.
D001234:Aspergillus niger / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D003176:Complement C3 / Q000378:metabolism; D003182:Complement C5 / Q000378:metabolism; D003168:Complement Fixation Tests; D003165:Complement System Proteins / Q000032:analysis / Q000378:metabolism*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D012995:Solubility; D013291:Streptococcus / Q000276:immunology*; D013295:Streptococcus mutans / Q000276:immunology*; D014867:Water
D003176:Complement C3; D003182:Complement C5; D011135:Polysaccharides, Bacterial; D014867:Water; D003165:Complement System Proteins