title: The removal of leukocytes and platelets from whole blood.
abstract: Various methods for the removal of leukocytes from whole blood have been compared and a new technique has been devised. This procedure consists of passing whole blood through a bed composed of microcrystalline cellulose and alpha-cellulose. The method is rapid, reliable, removes over 99.75 per cent of the leukocytes from blood, and does not seem selectively to retain reticulocytes or to release a significant proportion of leukocyte enzymes. Most of the platelets are also removed from anticoagulated blood, and platelet-free red cells can be obtained by passing defibrinated blood over the microcrystalline cellulose-alpha-cellulose bed.
mesh_terms: D001769:Blood; D001792:Blood Platelets; D002469:Cell Separation / Q000379:methods*; D002482:Cellulose; D002850:Chromatography, Gel; D004912:Erythrocytes; D005696:Galactosidases / Q000097:blood; D006405:Hematology / Q000379:methods; D006801:Humans; D007962:Leukocytes / Q000201:enzymology; D012156:Reticulocytes
chemical_list: D002482:Cellulose; D005696:Galactosidases