Placental cultures for cytogenetic assessment in saline-aborted fetuses.
Confirmation of cytogenetically abnormal fetuses following saline abortion has been shown to be possible with the placenta as the source of viable fetal cells. The method is described in detail. In one third of cultures, only female cells were present. Differentiation between maternal and female fetal tissue when no numerical or structural cytogenetic disorder is present requires detailed analysis of fluorescently stained chromosomes for polymorphisms.
D000028:Abortion, Induced / Q000379:methods*; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000175:diagnosis*; D025063:Chromosome Disorders; D003581:Cytodiagnosis; D005260:Female; D005333:Fetus / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008297:Male; D010920:Placenta / Q000166:cytology* / Q000187:drug effects; D011247:Pregnancy; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*
D012462:Saline Solution, Hypertonic; D012965:Sodium Chloride