A minicomputer-automated array spectrometer for liquid-chromatographic detection of metabolites.
A third-generation multiwavelength array spectrometer was developed as a detector for the high-resolution liquid-chromatographic characterization of metabolites. Components include a PDP-8/e minicomputer, matched pair of linear photodiode arrays, holographically-ruled gratings, fiber optics, flow cells, and high intensity xenon light source. The wavelength range is 256 nm differential with 1-nm resolution and can be adjusted from 200 to 800 nm. The system is capable of storing 20 spectra per second (200-456 nm) in a dual-beam mode. Special features include minicomputer-driven signal enhancement via integration as a function of signal strength. The display output includes presentation of the total absorption chromatogram vs. elution time in both real and post-run time as well as selectable single absorption band vs. elution time (post-run time). Application of this dedicated system is illustrated by the separation and charcterization of the metabolites of a carcinogen, 4-ethylsulfonyl-1 napthalenesulfonamide.
D000818:Animals; D001322:Autoanalysis; D002273:Carcinogens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D003201:Computers; D004848:Epithelium / Q000187:drug effects; D005260:Female; D006965:Hyperplasia / Q000139:chemically induced; D008433:Mathematics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008904:Miniaturization; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation*; D001743:Urinary Bladder / Q000187:drug effects; D014556:Urine / Q000032:analysis
D002273:Carcinogens