[Column chromatographic enrichment of "glucose-6-phosphate dehydrogenase vienna" (author's transl)].
Further biochemical investigations were performed in a case of glucose-6-phosphate dehydrogenase deficiency--called the "Vienna" deficiency variant on account of its biochemical characterization. It was found that the "Vienna" deficiency variant shows increased enzyme protein at a reduced specific activity. Gel filtration on Sephadex-G-200 produced a molecular weight identical with that of the normoenzyme; the different cochromatographic behaviour on hydroxylapatite appears attributable to a sequence modification of the amino acids.
D000328:Adult; D000595:Amino Acid Sequence; D001796:Blood Protein Disorders / Q000235:genetics; D001798:Blood Proteins / Q000032:analysis; D002847:Chromatography, Agarose; D004912:Erythrocytes / Q000201:enzymology*; D014644:Genetic Variation; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis*; D006529:Hepatomegaly / Q000209:etiology; D006801:Humans; D008297:Male; D008970:Molecular Weight; D013163:Splenomegaly / Q000209:etiology
D001798:Blood Proteins; D005954:Glucosephosphate Dehydrogenase
Säulenchromatographische Anreicherung der "Glukose-6-Phosphatdehydrogenase Wien"