Microcalorimetric determination of glucose in reference samples of serum.
Ireport a detailed series of microcalorimetric measurement of glucose concentrations in five reference samples of serum. The method utilized (a) calibration in the actual medium of analysis and (b) a correction for interferences owing to the nonspecificity of the enzyme hexokinase. The microcalorimetric results are compared with the tentative results of analyses obtained by the spectrophotometric hexokinase/glucose-6-phosphate dehydrogenase method and by isotope-dilution mass spectrometry. In most cases, the microcalorimetric results appear to be in agreement with the results obtained by these latter two methods. A discussion of the basis of the microcalorimetric measurements is presented.
D000255:Adenosine Triphosphate; D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D002151:Calorimetry / Q000379:methods; D002417:Cattle; D006593:Hexokinase; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008433:Mathematics; D008832:Microchemistry; D013053:Spectrophotometry / Q000379:methods
D001786:Blood Glucose; D000255:Adenosine Triphosphate; D006593:Hexokinase