title: Characterization of an Acholeplasma laidlawii variant with a REP- phenotype.
abstract: An Acholeplasma laidlawii variant has been isolated that has a REP- phenotype. The properties of this variant, relative to parental cells, are: (i) it exhibits no change in cell growth kinetics; (ii) it does not propagate single-stranded deoxyribonucleic acid (DNA) mycoplasmaviruses but does propagate double-stranded DNA mycoplasmaviruses; (iii) it converts parental circular single-stranded mycoplasmavirus DNA to double-stranded replicative forms that are not replicated further; (iv) it exhibits no change in host modification and restriction; and (v) it has an increased ultraviolet light sensitivity. The REP- isolate is the first stable mycoplasma variant to which a physiological defect has been attributed.
mesh_terms: D000128:Acholeplasma laidlawii / Q000528:radiation effects; D001435:Bacteriophages / Q000254:growth & development* / Q000378:metabolism; D004261:DNA Replication; D004270:DNA, Circular / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D014644:Genetic Variation; D010641:Phenotype; D011830:Radiation Effects; D014466:Ultraviolet Rays; D014779:Virus Replication
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral