Some properties of tobacco protoplast chromatin.
Chromatin was prepared from tobacco-leaf protoplasts. Its solubility in increasing molarities of NaCl was studied and the structure of the soluble fraction observed by electron microscopy. We demonstrate that in plants, the DNA and histones are associated in beaded structures similar to those called omicron-bodies or nucleosomes in animal chromatin. The nucleosomes were associated with DNA in either compact or extended forms. The compact arrangement was predominant in the fraction solubilized between 0.1 and 0.4 M NaCl. The extended form, present at 0.5 and 0.6 M NaCl. showed DNA filaments of various lengths interspacing the nucleosomes. At these ionic strengths ring structures were present, associated with the DNA. At 0.7 M NaCl and above, only DNA filaments were present, occasionally associated with big rings, and nucleosomes were compoetely dissociated. Free DNA molecules were present at all ionic strengths used. The possible origin and significance of the rings are discussed.
D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000648:ultrastructure; D004247:DNA; D006657:Histones / Q000032:analysis; D008836:Micrococcal Nuclease; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins; D009994:Osmolar Concentration; D010947:Plants, Toxic; D011523:Protoplasts; D012965:Sodium Chloride; D012995:Solubility; D014026:Nicotiana
D002843:Chromatin; D006657:Histones; D009698:Nucleoproteins; D012965:Sodium Chloride; D004247:DNA; D008836:Micrococcal Nuclease