title: Nuclear RNA in the mouse spleen at the peak of primary immune response.
abstract: Electrophoretic studies on nuclear RNA extracted from the spleens of immunized mice are reported. RNA was extracted by the phenol-detergent procedure from the purified nuclei of spleen cells at the peak of primary immune response after a single immunization with sheep red blood cells. The kinetics of labeling in vivo with 32P-orthophosphate indicate an accelerated biosynthesis of the ribosomal RNA precursors, 28S, 18S, 5S and 4S fractions of nuclear RNA in the spleens of immunized mice.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000276:immunology* / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000378:metabolism; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular; D007114:Immunization; D051379:Mice; D010710:Phosphates / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000276:immunology* / Q000378:metabolism
chemical_list: D010710:Phosphates; D010761:Phosphorus Radioisotopes; D012335:RNA, Ribosomal; D012313:RNA; D004247:DNA