[Potential difference across subcellular particle membranes. II. Compensation method of measurement].
"Zero-loop" of the molecular potential transformer of submitochondrial particles (SMP) is separated from the remaining electron transfer chain by rotenone, and its e.m.f. ET=0,003+RT/2F in [NADP X H] [NAD+]/[NADP+] [NAD X H] volts is used in the compensative method of measurement of the potential difference across the SMP membrane (delta USMP). The phospholipid membrane, measuring the concentration of the penetrating anions in the solution contained SMP, is used as "zero-indicators". This concentration drops monotonically with increase in delta USMP. Delta USMP is equal to ET when the addition of substrates of transhydrogenase reaction with definite ET does not change the potential across phospholipid membrane.
D000818:Animals; D066298:In Vitro Techniques; D008564:Membrane Potentials; D008722:Methods; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology / Q000502:physiology*
D009247:NADH, NADPH Oxidoreductases
Raznost' potentsialov na membrane subkletochnykh chastits. II. Kompensatsionnyĭ metod izmerniia