title: Preparation of concentrated platelet suspensions by dehydration against polyethyleneglycol 20,000.
abstract: Gel filtered platelet suspensions were concentrated by repeated dehydration against polyethyleneglycol 20,000 followed by dialysis against slightly hypotonic buffer. This method increased the platelet concentration 2-3 times with a recovery of 80-100%. The final cell suspension closely resembled the original platelet rich plasma as tested by a number of platelet tests.
mesh_terms: D000244:Adenosine Diphosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001772:Blood Cell Count; D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure; D002469:Cell Separation; D002850:Chromatography, Gel / Q000379:methods*; D006019:Glycolysis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007773:Lactates / Q000097:blood; D010101:Oxygen Consumption; D010977:Platelet Factor 3 / Q000032:analysis; D011092:Polyethylene Glycols; D012701:Serotonin / Q000097:blood; D013535:Suspensions
chemical_list: D007773:Lactates; D013535:Suspensions; D012701:Serotonin; D010977:Platelet Factor 3; D011092:Polyethylene Glycols; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase