title: Light-induced absorption changes in photosystem I at low temperatures.
abstract: Light-induced absorption changes associated with the primary photochemical reaction and dark relaxation in Photosystem I were measured at various low temperatures. A possible temperature-dependent long-range electron tunneling process was suggested to account for the unique temperature dependence of the dark decay process. The kinetics of the light-induced absorption changes are in good agreement with the light-induced EPR changes reported earlier (Ke, B., Sugahara, K., Shaw, E.R., Hansen, R. E., Hamilton, W. D. and Beinert, H. (1974) Biochim, Biophys. Acta 368, 401--408) for the same Photosystem I subchloroplast fragment at comparable temperatures. All absorption changes between 400 and 725 nm at 86degreesK have identical kinetics. The light-minus-dark difference spectrum is very similar to that of P-700 at room temperature, with an additional prominent positive change at 690 nm. Possible contributions by P-430 to the blue and red spectral changes were discussed. It was demonstrated that the intensity of the measuring beam has a drastic effect on the light-induced absorption changes of Photosystem I at low temperatures. Various pretreatments of the Photosystem I fragments such as those that photochemically (or chemically) oxidize the primary donor or photoreduce the primary acceptor abolish the subsequent photochemical reaction. Continuous illumination of the Photosystem I fragments before and during freezing has the same effect. In the temperature range of --20 to --60degreesC, an unusual counter absorption change as well as a counter EPR change were observed.
mesh_terms: D015086:2,6-Dichloroindophenol / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002736:Chloroplasts / Q000528:radiation effects; D005615:Freezing; D007700:Kinetics; D008027:Light; D010788:Photosynthesis / Q000528:radiation effects*; D013053:Spectrophotometry; D013696:Temperature; D013772:Tetramethylphenylenediamine / Q000494:pharmacology
chemical_list: D015086:2,6-Dichloroindophenol; D013772:Tetramethylphenylenediamine; D001205:Ascorbic Acid