Profiles of volatile metabolities in body fluids.
A method for the analysis of volatile metabolites present in plasma, urine, breast milk and amniotic fluid collected from mother-infant pairs has been developed which requires only 100 mul of plasma, 3 ml of urine, 20 mul of breast milk and 500 mul of amniotic fluid. After extraction with diethyl ether, the volatile compounds were absorbed on glass wool in a special concentration tube and subsequently desorbed and transferred to a 100-m nickel capillary column for analysis by gas chromatography and gas chromatography-mass spectrometry. The separations, carried out by temperature programming, were complete in 90 min.
D000438:Alcohols / Q000032:analysis / Q000097:blood / Q000652:urine; D000653:Amniotic Fluid / Q000032:analysis; D001826:Body Fluids / Q000032:analysis*; D002849:Chromatography, Gas; D003510:Cyclohexanes / Q000032:analysis / Q000097:blood / Q000652:urine; D005260:Female; D005663:Furans / Q000032:analysis / Q000097:blood / Q000652:urine; D005740:Gases / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D013058:Mass Spectrometry; D008895:Milk, Human / Q000032:analysis; D013696:Temperature; D014835:Volatilization
D000438:Alcohols; D003510:Cyclohexanes; D005663:Furans; D005740:Gases