title: The influence of in-vitro culture and cooling on the survival and development of cow embryos.
abstract: Cow morulae cultured in a phosphate-buffered medium containing serum developed normally and retained viability when transferred to recipients. Unlike earlier cleavage stages, cow blastocysts tolerated cooling to 0 degrees C and retained viability after storage for 48 hr at 0 degrees C when transferred to recipients.
mesh_terms: D000367:Age Factors; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle / Q000196:embryology*; D046508:Culture Techniques; D004624:Embryo Transfer; D005260:Female; D012034:Refrigeration; D014022:Tissue Survival; D014184:Transplantation, Homologous