title: The phosphopyridoxyl peptide from the mitochondrial aspartate aminotransferase of beef kidney.
abstract: The sequence around the coenzyme-binding lysine of mitochondrial aspartate amino-transferase from beef kidney was determined. The holoenzyme was treated with NaB3H4 and digested with thermolysin; the labelled peptide was isolated and its sequence proved to be identical with sequences around the coenzyme site from the same isoenzyme of different organs and animals (pig heart and sheep liver). The sequences of the phosphopyridoxyl peptides of the mitochondrial aspartate aminotransferases appear to be closely related to the corresponding peptides from the cytoplasmic isoenzymes.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis* / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D012756:Sheep; D013552:Swine; D013820:Thermolysin / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D007527:Isoenzymes; D010455:Peptides; D001219:Aspartate Aminotransferases; D013820:Thermolysin