Lack of correlation between agglutinability, the surface distribution of con A and post-confluence inhibition of cell division in ten cell lines.
Agglutinability by concanavalin A, distribution of surface-bound concanavalin A, and maximal cell density in monolayer culture were examined under similar conditions in parallel cultures of ten established cell lines. The degree of agglutinability of the cell lines did not correlate with the presence or absence of patching of concanavalin A bound to the cell surface, as determined with a hemocyanin marker. Agglutinability was also not always correlated with the loss of post-confluence inhibition of cell division. Two clones of mouse 3T3 fibroblasts that maintained post-confluence inhibition of cell division and low agglutinability differed substantially with respect to the surface distribution of concanavalin A. Patching of concanavalin A binding sites is neither necessary nor sufficient to explain differences in agglutinability between cell lines.
D000371:Agglutination; D001665:Binding Sites; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D003208:Concanavalin A / Q000378:metabolism*; D003260:Contact Inhibition
D003208:Concanavalin A