A chemical assay method for the determination of aflatoxin residues in animal tissues.
A method is presented for the determination of aflatoxins in animal tissues. It consists of extraction of aflatoxins from the sample with methanol, treatment of the residue with a mixture of dimethoxymethane-methanol (4+1) to further precipitate proteins, evaporation of dimethoxymethane followed by a liquid-liquid defatting with hexane and heating of the methanol-water mixture before the transfer of aflatoxins into chloroform. Chloroform extracts are further purified by silica gel--acidic alumina--anhydrous sodium sulfate column chromatography.
D000273:Adipose Tissue / Q000378:metabolism; D000348:Aflatoxins / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D005506:Food Contamination / Q000032:analysis*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D011200:Poultry; D013552:Swine
D000348:Aflatoxins