Preparation and properties of tRNA from human placenta.
1. The tRNA preparation obtained from human placenta by phenol extraction and DEAE-cellulose chromatography was homogeneous on ultracentrifugation and showed high amino acid acceptor activity. 2. The analysis of the isoaccepting tRNA by reversed-phase chromatography (RPC-5 system) showed the presence of 5 fractions for glycine and leucine, 3 for tyrosine, alanine and valine, 2 for arginine and 1 for phenylalanine.
D000596:Amino Acids / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D005260:Female; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D009994:Osmolar Concentration; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D012343:RNA, Transfer / Q000302:isolation & purification / Q000378:metabolism; D013329:Structure-Activity Relationship; D046249:Transfer RNA Aminoacylation
D000596:Amino Acids; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D008274:Magnesium