[Determining hypersensitivity to antigens by computing cells migrating from capillary tubes].
The test technique of inhibition of leukocyte and macrophage migration from the capillaries in the presence of a specific antigen is described. The method is highly sensitive and permitted to use a small amount of cells and many (up to 600 and more) capillaries. Sodium azide, phitohemagglutinin and incubation at 4 degrees C inhibited the cell migration from the capillaries. Dead cells did not pass from the capillaries into the medium. Tuberculin and BCG vaccine inhibited the migration of macrophages of guinea pigs immunized with BCG, but not with staphylococcus allergen.
D000818:Animals; D001386:Azides / Q000494:pharmacology; D001500:BCG Vaccine; D002452:Cell Count; D002464:Cell Migration Inhibition; D002465:Cell Movement / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000175:diagnosis*; D037102:Lectins / Q000494:pharmacology; D007962:Leukocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D013203:Staphylococcal Infections / Q000276:immunology; D013696:Temperature; D013997:Time Factors; D014373:Tuberculin; D014611:Vaccination
D001386:Azides; D001500:BCG Vaccine; D037102:Lectins; D014373:Tuberculin; D000431:Ethanol
Opredelenie giperchuvstvitel'nosti k antigenam putem podscheta kletok, migrirovavshikh iz kapilliarnykh trubok