Effects of ionizing radiation of RNA metabolism in cultured mammalian cells. III. Specific reduction in mRNA synthesis during resumed division and plateau phases following exposure to 800 rads of x irradiation.
The cell growth response of cultured Chinese hamster ovary cells, line CHO, to 800 rads of X irradiation involves a period of division delay, followed by a period of resumed division which terminates in a plateau phase. Over 95% of the cells die eventually. No direct effects of RNA or protein metabolism are evident during the delay period. During the resumed division and beginning of plateau phases, however, a specific and relatively constant reduction in mRNA synthesis relative to messenger-like RNA and heterogenous nuclear RNA synthesis is evidenced. The ratio of mRNA to messenger-like RNA synthesis ranges from 0.8 to 0.65 during these phases. The effect is not due to altered cell-cycle distribution, and evidence is presented to indicate that it is probably not a compensatory response to the unbalanced growth that occurs during the division delay period.
D000241:Adenosine / Q000378:metabolism; D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured / Q000378:metabolism / Q000528:radiation effects*; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D013936:Thymidine / Q000494:pharmacology; D013997:Time Factors; D014529:Uridine / Q000378:metabolism; D014965:X-Rays
D012333:RNA, Messenger; D012261:Ribonucleoproteins; D012313:RNA; D004247:DNA; D012343:RNA, Transfer; D000241:Adenosine; D013936:Thymidine; D014529:Uridine