[Influence of dexamethasone on RNA synthesis in human peripheral blood lymphocytes].
Biphasic character of dexamethasone action on the lymphoid tissue is observed in the tissue culture of partially purified peripheral blood lymphocytes. Stimulation of the RNA synthesis occurred after 6-hour incubation of lymphocytes with the hormone. Sedimentation analysis of labeled RNA, fractionated in a column containing poly-U sepharose pointed to increase in the quantity of the messenger RNA and enrichment of the cytoplasmic RNA with poly-A sequences. At the same time the Mn++-dependable RNA polymerase sensitive to alpha-amanitin was activated. Inhibition of the RNA synthesis is observed after 24-hour cultivation of lymphocytes with the hormone. Biphasic effect of the steroid is also seen in the reaction of rosetteformation.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008345:Manganese; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*
D011061:Poly A; D008345:Manganese; D012313:RNA; D003907:Dexamethasone; D012321:DNA-Directed RNA Polymerases
Vliianie deksametazona na sintex RNK v limfotsitakh perifericheskoĭ krovi cheloveka