Kinetic analysis of penicillin degradation in acidic media.
Degradation of penicillin in acidic media (pH 2.7) was monitored by high-pressure liquid chromatography and UV spectroscopy. The effects of temperature, buffer concentration, and ionic strength were examined. A degradation pathway is proposed, and the apparent first-order rate constant and energy of activation were calculated for each reaction. One or more degradation products containing a sulfhydryl group, a functional group often suggested as having a major role in eliciting allergic responses to penicillin therapy, were present throughout the degradation scheme.
D000143:Acids; D002021:Buffers; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D007700:Kinetics; D009994:Osmolar Concentration; D010406:Penicillins / Q000032:analysis; D013696:Temperature
D000143:Acids; D002021:Buffers; D010406:Penicillins