Effect of hypoxia on erythroblasts from avian fetal liver: adenosine triphosphate levels and hemoglobin synthesis.
A system for the isolation and functional evaluation of fetal liver erythroblasts is described. Isolated erythroblasts were prepared from 14-day embryonic avian livers and incubated at various oxygen tension (0, 5, 12, and 95%). The concentration of ATP in erythroblasts remained constant for at least 4 hr at 37degree, but was rapidly reduced by incubation in nitrogen. Protein synthesis as measured by L-[14C]leucine incorporation into cell protein occurred at a linear rate in 5%, 12%, and 95% oxygen, whereas little protein synthesis occurred at 0% oxygen. The effect of hypoxia on the type of hemoglobin synthesized was studied in this system by isolating the hemoglobin A, hemoglobin D, and hemoglobin H fractions and determining the incorporation of L-[14C]leucine. The major fraction, hemoglobin A, contained most of the radioactivity; smaller amounts were present in hemoglobin D and hemoglobin H, respectively. The relative proportion of each hemoglobin synthesized was not altered by oxygen from 5% to 95%. These results argue against a direct effect of oxygen on the type of hemoglobin synthesized at this stage of development.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002642:Chick Embryo; D004900:Erythroblasts / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D004920:Erythropoiesis; D004921:Erythropoietin / Q000502:physiology; D006454:Hemoglobins / Q000096:biosynthesis*; D000860:Hypoxia / Q000378:metabolism*; D008099:Liver / Q000196:embryology / Q000378:metabolism
D006454:Hemoglobins; D004921:Erythropoietin; D000255:Adenosine Triphosphate