title: Crystal structure of elastase-substrate complex at -- 55 degrees C.
abstract: The structure of a specific acyl-enzyme intermediate in the elastase-catalysed hydrolysis of N-carbobenzoxy-L-alanyl-p-nitrophenol ester has been determined by X-ray diffraction at 3.5 A resolution. The acyl-enzyme was stabilised by cooling the crystal to --55 degrees C during substrate addition and data collection.
mesh_terms: D001665:Binding Sites; D003080:Cold Temperature; D003460:Crystallization; D009596:Nitrophenols / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: D009596:Nitrophenols; D010196:Pancreatic Elastase