Immunochemical analysis of an unusual cell wall polysaccharide from animal coagulase-positive staphylococci. 1. Fragments obtained after hydrolysis in hydrofluoric acid and alkali.
Polysaccharide P (poly P) of canine coagulase-positive staphylococci contains glycerol, glucose, glucosamine, muramic acid, phosphate, and the usual peptidoglycan amino acids, but does not cross-react serologically with standard teichoic acids. Products from hydrolyses in hydrofluoric acid and alkali contained phosphates of glycerol and glucose as well as combinations of these, but neither glucosyl-glycerol units nor glucosamine-phosphates were observed. The teichoic acid of poly P is probably a polymer of a repeating unit consisting of alternating glycerol, phosphate and glucose.
D000468:Alkalies; D000596:Amino Acids / Q000032:analysis; D002473:Cell Wall / Q000032:analysis*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D003030:Coagulase; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D005959:Glucosidases; D005990:Glycerol / Q000032:analysis; D006596:Hexosaminidases; D006858:Hydrofluoric Acid; D006868:Hydrolysis; D009112:Muramic Acids / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D013211:Staphylococcus aureus / Q000032:analysis*; D013682:Teichoic Acids
D000468:Alkalies; D000596:Amino Acids; D003030:Coagulase; D009112:Muramic Acids; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D013682:Teichoic Acids; D005959:Glucosidases; D006596:Hexosaminidases; D005947:Glucose; D005944:Glucosamine; D005990:Glycerol; D006858:Hydrofluoric Acid