Anti-platelet antibody determination by platelet factor 3 assay.
Anti-platelet antibody determinations by a recently introduced platelet factor 3 assay (Pf-3 assay) were evaluated in 159 specimens from 95 patients who had low platelet counts. As a standard procedure, the 51Cr lysis test (51Cr test) was used. The 51Cr test and Pf-3 assay had an excellent correlation (results agreed for 80.5% of 159 specimens tested). Overall positive results for the 159 specimens were 27.7% by the 51Cr test and 22.0% by the Pf-3 assay. A significantly high incidence of anti-platelet antibodies (71.4%) was found in patients who had received more than 10 units of blood transfusions, in contrast to a non-transfused group, which had an incidence of 6.3%. The difference between transfused and non-transfused groups was significant (x2 = 11.854, p less than 0.001). The Pf-3 assay is easy to perform in most laboratories and yet sensitive enough to detect anti-platelet antibodies that were detectable by the 51Cr test.
D000328:Adult; D000906:Antibodies / Q000032:analysis*; D001779:Blood Coagulation Factors / Q000032:analysis*; D001792:Blood Platelets / Q000276:immunology*; D001803:Blood Transfusion; D002860:Chromium Radioisotopes; D005260:Female; D006801:Humans; D007938:Leukemia / Q000276:immunology; D008107:Liver Diseases / Q000276:immunology; D008297:Male; D008875:Middle Aged; D004364:Pharmaceutical Preparations / Q000276:immunology; D010977:Platelet Factor 3 / Q000032:analysis*
D000906:Antibodies; D001779:Blood Coagulation Factors; D002860:Chromium Radioisotopes; D004364:Pharmaceutical Preparations; D010977:Platelet Factor 3