title: [Protein crystals and tubuli bundles in yeasts cells. III. Identification of crystals as alcohol dehydrogenase (ADH)].
abstract: Inducibility of alcohol dehydrogenase (ADH) crystals in yeast cells depends on the growth phase. They accumulate during exponential growth, a process that is parallelled by accumulation of a special protein (H protein). The following pieces of evidence show that crystals, H protein, and ADH are identical: (1) The three proteins have the same amino acid composition and subunit molecular weight; (2) active H protein and ADH exhibit the same electrophoretic mobility, indicating a common molecular weight of about 158 000 D; (3) the native H protein and a crystal fraction stabilized by Zn++ and dialyzed against EDTA possess ADH activity.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D003461:Crystallography; D005656:Fungal Proteins / Q000032:analysis*; D008970:Molecular Weight; D012440:Saccharomyces / Q000254:growth & development / Q000648:ultrastructure*
chemical_list: D000596:Amino Acids; D005656:Fungal Proteins; D000429:Alcohol Oxidoreductases
vernacular_title: Proteinskristalle und Tubuli-Bündel in Hefezellen. III. Identifizierung der Kristalle als Alkoholdehydrogenase (ADH)