The effects of mazindol and 46-034 (Sandoz) on glucose oxidation and insulin binding by rat isolated fat cells.
1. The anorexic agent mazindol and its major metabolite 46-034 (Sandoz) in high concentrations ( greater than 0-4 mM) abolished basal and insulin-stimulated conversion of 1-14C-glucose to 14CO2 by rat isolated fat cells. 2. High concentrations (1mM) also inhibited specific binding of 125 I-insulin to fat cells. 3. The observed effects appeared to be due in part to perturbation of the plasma membrane since there was a rise in the lactate dehydrogenase content of the incubation medium, increased 125I-insulin degradation and a reduction in cellular tritiated water space. 4. These effects are unlikely to be relevant to the therapeutic action of mazindol.
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D002477:Cells; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008454:Mazindol / Q000031:analogs & derivatives / Q000494:pharmacology*; D010084:Oxidation-Reduction; D051381:Rats
D007211:Indoles; D007328:Insulin; D007457:Iodine Radioisotopes; D008454:Mazindol; D007770:L-Lactate Dehydrogenase; D005947:Glucose