title: Studies on in vitro synthesis and secretion of human chorionic gonadtropin and its subunits.
abstract: Human chorionic tissues were cultivated in vitro in they presence of 3H-proline in order to study the synthesis of the hCG and its subunits by the placenta. After terminating the culture, tissue extracts and media were individually gel-filtrated on Sephadex G-100. The eluted fractions were radioimmunoassayed for hCG, hCGalpha and hCGbeta and were measured for 3H-radioactivity. Label incorporation was determined by immunoprecipitation. Elution profiles of tissue extracts showed the existence of large immunologic forms of hHCG, hCGalpha and hCGbeta emerging near the void volume. The amounts of these large immunologic species in chorionic tissue gradually decreased during the course of cultivation. 3H-proline was almost exclusively incorporated into the large immunologic forms of hCG, hCGalpha and hCGbeta within the chrionic tissue during the 5-hour exposure. A great quantity of hCGalpha was found in the media after the 3-day culture, while the amount of hCGbeta found in the media was minute. After a 15-minute pulse, the 3H-radioactivity peak within the chorionic tissue appeared in the void volume, conicidental with hCG immunoreactivity. During the chase period, there was shift of the 3H-radioactivity peak associated with hCG immunoreactivity from the void volume to the more retarded area. In the media until after the 60-minute chase, no labeled hCG and its subunits appeared. Within the media after 3-hour chase, no labeled hCG peak associated with 3H-radioactivity was more retarded on Sephadex G-100 than that within the tissue extract after the 15-MINUTE pulse. These results suggest that the large immunologic forms of hCG, HCGalpha and hCGbeta are synthesized as the earliest detectable biosynthetic forms and that the may then be converted to small molecule species. In the culture of molar trophoblastic tissues after a 15-minute pulse, considerable amounts of hCG and its subunits accompanied by high 3H-radioactively had already been secreted into the media. These observations suggest that protein synthesis by molar trophoblastic tissue is markedly enhanced as compared with that by normal chorionic tissue and that immunoreactive materials synthesized in molar trophoblastic tissue may be secreted more radily than those synthesized a in normal chorionic tssue.
mesh_terms: D006063:Chorionic Gonadotropin / Q000096:biosynthesis*; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000378:metabolism; D066298:In Vitro Techniques; D010446:Peptide Fragments / Q000096:biosynthesis; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011392:Proline / Q000378:metabolism; D011863:Radioimmunoassay; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin; D010446:Peptide Fragments; D011392:Proline