[Specificity of thermolysin action on dog myoglobin].
The proteolytic specificity of thermolysin has been studied by quantitative analysis of an enzymic digest of dog myoglobin. Results confirm main specificities of thermolysin towards Phenylalanine, Isoleucine, Leucine or Tyrosine bonds; the influence of neighbourhood was also determined and the conclusions are in a good agreement with the known structure of the active site of thermolysin.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D004151:Dipeptides / Q000032:analysis; D004285:Dogs; D007532:Isoleucine; D009211:Myoglobin; D010446:Peptide Fragments / Q000032:analysis; D010649:Phenylalanine; D011485:Protein Binding; D013329:Structure-Activity Relationship; D013820:Thermolysin / Q000378:metabolism; D014443:Tyrosine
D004151:Dipeptides; D009211:Myoglobin; D010446:Peptide Fragments; D007532:Isoleucine; D014443:Tyrosine; D010649:Phenylalanine; D013820:Thermolysin
Spécificité d'action de la thermolysine sur la myoglobine de chien