A simple fluorometric assay for type B monoamine oxidase activity in rat tissues.
A simple fluorometric assay for monoamine oxidase (MAO) [EC 1.4.3.4] activity towards beta-phenylethylamine (PEA) was devised. The procedure consists in measuring the disappearance of PEA fluorometrically. The disappearance of PEA was completely inhibited by pargyline, a potent inhibitor of MAO. MAO activity for PEA was linear with 10 mg to 100 mg of liver tissue in 3 ml of reaction mixture for up to 90 min of incubation. Using this method, the V max values and the apparent Km values of MAO for PEA in several rat tissues were determined, and compared with those for benzylamine and 5-hydroxytryptamine (5-HT).
D000818:Animals; D001921:Brain / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008995:Monoamine Oxidase / Q000032:analysis*; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D010293:Pargyline / Q000494:pharmacology; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods
D010293:Pargyline; D008995:Monoamine Oxidase