title: Quantitative precorneal disposition of topically applied pilocarpine nitrate in rabbit eyes.
abstract: The present study was designed to quantitate the influence of several precorneal factors on the disposition of topically applied ophthalmic drugs. With tritiated pilocarpine nitrate methodology was developed for in vivo assessment of the relative contribution of tear turnover, instilled solution drainage, and nonproductive absorption to the loss of drug from the precorneal area. Studies were conducted in both awake and anesthetized rabbits whose drainage ducts were either unobstructed or plugged, and the loss of drug was monitored directly from the precorneal area or as appearance in the aqueous humor. By selective variation in experimental conditions, the influence of tear turnover, instilled solution drainage, and nonproductive absorption on ocular drug bioavailability was separately studied and quantitated. Instilled solution drainage was by far the largest contributing factor in the loss of drug from the precorneal area of the eye and, in the range of instilled volumes normally employed, tear turnover played a relatively minor role in drug loss. Compared to the cornea, precorneal tissue other than the cornea has a considerably greater surface area and thus is a potentially signifanct route for drug loss. However, under normal circumstances, loss by this route was minimal as compared to loss via instilled solution drainage.
mesh_terms: D000287:Administration, Topical; D000768:Anesthesia, General / Q000009:adverse effects; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D001682:Biological Availability; D003315:Cornea / Q000378:metabolism*; D007700:Kinetics; D007765:Lacrimal Apparatus / Q000502:physiology; D008297:Male; D010862:Pilocarpine / Q000008:administration & dosage / Q000378:metabolism*; D011817:Rabbits; D013666:Tears / Q000378:metabolism
chemical_list: D010862:Pilocarpine