Immunoelectroosmophoresis for detection of reo-like virus: methodology and comparison with electron microscopy.
In two separate studies, faecal samples were collected from children with acute gastroenteritis; the samples were tested both by electron microscopy and immunoelectroosmophoresis with a view to detect reo-like virus. In 94 per cent and 100 per cent of samples positive by electron microscopy in the first and second material, respectively, a precipitin line was found after electrophoresis, using a guinea pig antiserum against a purified bovine virus. No false positives were detected by this method.
D002648:Child; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology*; D006801:Humans; D007122:Immunoelectrophoresis; D008854:Microscopy, Electron; D012087:Reoviridae / Q000302:isolation & purification*