The quantification of formazans in tissue sections by microdensitometry. II. The use of BPST, a new tetrazolium salt.
This article describes the use of a microdensitometer for the measurement of BPST formazan in tissue sections. BPST is a new tetrazolium salt, 2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium chloride, which produces a single, well-defined formazan, and is thus easily quantified. The formazan gives an excellent localization, since BPST was originally designed for ultrastructural work. Activities are expressed in absolute units as n moles hydrogen/mm3, and are thus directly comparable with standard biochemical data.
D000818:Animals; D001391:Azo Compounds / Q000032:analysis*; D005562:Formazans / Q000032:analysis*; D008099:Liver / Q000032:analysis / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008832:Microchemistry; D013053:Spectrophotometry / Q000379:methods; D013778:Tetrazolium Salts
D001391:Azo Compounds; D005562:Formazans; D013778:Tetrazolium Salts