Effect of different lysing and washing methods on free amino acid concentrations of sheep whole blood and erythrocytes.
1. A study was conducted to determine the effect of different washing and lysing methods on the free amino acid concentrations of sheep erythryocytes (RBC) and whole blood. Methods of lysis included laking, freeze-thawing or sonication. RBC were washed with isotonic solutions of either saline or sucrose. 2. Washing the RBC with isotonic sucrose resulted in significantly greater concentrations for ten of the amino acids. Greater amino acid concentrations were realized when washed RBC were sonicated and when the RBC of whole blood were lysed by laking. 3. Suggestions are made for future application to whole blood and RBC amino acid determinations.
D000596:Amino Acids / Q000097:blood*; D000818:Animals; D004912:Erythrocytes / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006461:Hemolysis; D008722:Methods; D012756:Sheep
D000596:Amino Acids