Examination and Photography of donor corneal endothelium.
In a method for the routine examination of the endothelium of donor corneas before transplantation, the donor endothelial cells are examined and photographed as they undergo preservation or culture in liquid media. A specular microscope with a modified objective lens is used (working distance, approximately 9 mm). The donor corneas are kept in a special storage container that allows a direct view of the endothelium from outside the container. The endothelium of any cornea preserved in a liquid medium may thus be examined and photographed at high magnification without opening the storage container.
D003315:Cornea / Q000648:ultrastructure*; D004727:Endothelium / Q000648:ultrastructure; D005125:Eye Banks; D006801:Humans; D008853:Microscopy / Q000379:methods; D010781:Photography / Q000379:methods