[Physico-chemical characterisation and crystallisation of immunoglobulins and their fragments: a contribution to the three dimensional structural determination of antibodies, III. Human immunoglobulin Kol, a myeloma protein of the type IgG, gamma2 kappa2, Gm (f)+. Comparative studies on dissolved and crystalline material (author's transl)].
Immunoglobulin Kol has been isolated and characterised as the intact molecule, which consists of an antigen binding part (Fab) and the C-terminal fragment (Fc). Crystals of the protein are suitable for X-ray diffraction resolution of the fine structure, down to atomic dimensions; but this does not permit the representation of the Fc-part of the molecule. The following studies were performed on the protein in solution before and after crystallisation; the results were the same in both cases, thus showing that crystallisation does not alter the molecule: After enzymic cleavage of the protein, the products were isolated and identified by immunological reactions and by analytical ultracentrifugation; the behaviour of the protein in disc electrophoresis following reductive cleavage was also studied.
D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003460:Crystallization; D004589:Electrophoresis, Disc; D006801:Humans; D006868:Hydrolysis; D007122:Immunoelectrophoresis; D007140:Immunoglobulin Fab Fragments / Q000032:analysis; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D009194:Myeloma Proteins / Q000032:analysis / Q000302:isolation & purification; D010206:Papain; D014461:Ultracentrifugation
D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D009194:Myeloma Proteins; D010206:Papain
Uber die physikalisch-chemische Charakterisierung und nachfolgende Kristallisation von Immunglobulinen und deren Fragmenten: Ein Beitrag zur dreidimensionalen Strukturaufklärung der Antikörper, III. Das humane Immunglobulin Kol, ein Myelom-Protein der Klasse IgG, gamma2 kappa2, Gm (f)+; vergleichende. Untersuchungen am gelösten und kristallinen Material