title: Reliability of copper reduction methods for quantitation of glucosuria.
abstract: In the present study we evaluated the reliability of the "2-drop" Clinitest method in determining the 24-h glucose spill of a diabetic patient. The urine glucose content over the 33 day study period was measured with a Beckman glucose analyzer that employed a glucose oxidase method. There was no statistical difference between the glucose content determined by the two methods. There was a mean error of +4.4 +/- 3.6% between the values obtained with the "2-drop" method and values obtained with glucose analyzer. The mean coefficient of variation for determining the glucose content of urine with the "2 drop" method was 19%. In a group of 10 normal volunteers we found that there was a falsely elevated glucose value in urines with osmolality of less than 295 mosm/kg when the "5-drop" method was used. The "2-drop" Clinitest and the Beckman analyzer could accurately determine glucose concentration even in these dilute urines. The "2-drop" Clinitest method is a useful and reasonably reliable method for evaluationg 24-h glucosuria.
mesh_terms: D000328:Adult; D003300:Copper; D003920:Diabetes Mellitus / Q000652:urine*; D005069:Evaluation Studies as Topic; D005260:Female; D005949:Glucose Oxidase; D006029:Glycosuria / Q000175:diagnosis*; D006801:Humans; D009994:Osmolar Concentration; D010084:Oxidation-Reduction
chemical_list: D003300:Copper; D005949:Glucose Oxidase