Protein metabolism in burned rats.
Incorporation of [2-14C]glycine was used to estimate serum protein synthesis in four groups of rats. These were the control (group C); 20% body surface burn (group B); 20% burn, seeded with Pseudomonas aeruginosa (group BI); and burned-infected treated topically with mafenide (alpha-amino-p-toluenesulfonamide) acetate (group BIS), a treatment which controls P, aeruginosa burn-wound infection in humans. On the 6th day postburn the relative specific activities of all fractions were increased in the order BI greater than BIS greater than B greater than C, as were the concentrations of the globulins; Serum albumin concentration fell, being lowest in BI. Tissue albumin contents, measured by radioimmunoassay, of eviscerated blood-free bodies of rats were (mg/100 g rat wt): C, 207; B, 294; BI, 256. Analyses of individual tissues showed that the difference was due to increased albumin content in the burn-wound area. The tissue albumin was of normal molecular size and was immunologically reactive. We conclude that the prolonged hypoalbuminemia following burn injury is not a consequence of impaired albumin synthesis, but a result of altered compartmentation.
D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis / Q000378:metabolism*; D001835:Body Weight; D002056:Burns / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D008272:Mafenide / Q000494:pharmacology; D008297:Male; D009132:Muscles / Q000378:metabolism; D011552:Pseudomonas Infections / Q000378:metabolism; D051381:Rats; D012709:Serum Albumin / Q000096:biosynthesis / Q000378:metabolism*; D014867:Water / Q000378:metabolism
D001798:Blood Proteins; D012709:Serum Albumin; D014867:Water; D008272:Mafenide; D005998:Glycine