Rapid and effective separation of thyroid hormones and iodide by thin-layer chromatography.
The use of 1,3-dichlorotetrafluoroacetone allows the conversion of the iodoamino acids into a chemical form convenient for following chromatographic separation on silica as an adsorbent and benzene or chloroform as a solvent. The time required for derivatization and TLC separation is less than one hour. The outstanding resolution of the substance of interest, as well as the sensitivity of the radiochemical analysis, makes the method convenient for the rapid determination of minute quantities of thyroid hormones.
D002855:Chromatography, Thin Layer / Q000379:methods; D006801:Humans; D007454:Iodides / Q000302:isolation & purification*; D009132:Muscles / Q000378:metabolism; D013974:Thyroxine / Q000302:isolation & purification* / Q000378:metabolism; D013997:Time Factors; D014284:Triiodothyronine / Q000302:isolation & purification*
D007454:Iodides; D014284:Triiodothyronine; D013974:Thyroxine