[Comparative characteristics of ribonuclease isolated from the lliver and pancreas of burned and intact rats].
Alkaline RNA-ase was isolated from the pancreas and liver of intact and burnt rats, and purified 1400 and 2000 times accordingly. The optimum of the enzyme activity was achieved at pH 7.6-7.8. Normal and post-burn RNA-ase biosynthesis was studied using radioamino acids (C14-lysine and C14-glycine). Burnt animals displayed a slowed down alkaline RNA-ase biosynthesis, both in the liver and in the pancreas. Ribonuclease "half-life periods" of the liver of intact rats was 65 min, whereas in that of the burnt animals it was prolonged to 100.7 min. The end-preparation activity of the liver and pancreas alkaline RNA-ase of the intact and burnt animals showed no significant difference.
D000818:Animals; D002056:Burns / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism*
D012260:Ribonucleases
Sravnitel'naia kharakteristika ribonukleazy, vydelennoĭ iz pecheni i podzheludochnoĭ zhelezy obozhzhennykh i intaktnykh krys