Gas-liquid chromatography of amino acids. Determination of cystine and cysteine as N-acetyl, n-propyl S-carboxymethylcysteinate.
A procedure is described for the derivatisation of S-carboxymethylcysteine yielding the corresponding N-acetyl, n-propyl S-carboxymethylcysteinate which is separated by gas chromatography form similar derivatives of other protein amino acids. Its quantitative determination provides a useful extension to a method for analysis of amino acids by gas chromatography and offers potential as a means of obviating errors in cystine (and/or cysteine) analysis arising from acid hydrolysis of proteins.
D002233:Carbocysteine / Q000031:analogs & derivatives*; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis*; D003553:Cystine / Q000032:analysis*; D008722:Methods; D011492:Protein Hydrolysates / Q000032:analysis
D011492:Protein Hydrolysates; D003553:Cystine; D002233:Carbocysteine; D003545:Cysteine