Nonparticipation of adrenergic mechanism in potassium-induced relaxation of taenia from guinea pig caecum.
In the isolated taenia caeci of guinea pigs excess potassium (10-30 mM) induced a phasic relaxation followed by a contraction. This phasic relaxation was unaffected by treatment with  hexamethonium, phentolamine, propranolol and bretylium. However, relaxation induced by perivascular nerve stimulation was inhibited by all these agents but not by hexamethonium. Tetrodotoxin inhibited both forms of relaxation. Potassium-induced relaxation was not accompanied by [3H]noradrenaline release. Perivascular nerve stimulation caused release of [3H]noradrenaline and this was blocked by bretylium. Treatment with ouabain or replacement of NaCl by LiCl, but not treatment by cold storage, inhibited the potassium-induced relaxation. These results suggest that the potassium-induced relaxation of taenia caeci was due to electrogenic sodium pumping and was independent of the adrenergic innervation of the tissue.
D000818:Animals; D002432:Cecum / Q000187:drug effects / Q000502:physiology; D003080:Cold Temperature; D004558:Electric Stimulation; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D011188:Potassium / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D013564:Sympathetic Nervous System / Q000502:physiology*
D011189:Potassium Chloride; D011188:Potassium