[The automated kinetic determination of enzyme activities and constituents of plants. 3. Determination of bromelain (author's transl)].
With bromelain as protease model a kinetic method, capable of automatization, is described for the assay of proteolytic activities. The method uses milk protein as a substrate. The volume of the test mixture is fixed to 1 ml; the readings are performed at 340 nm. With the bromelain preparation used (2 mAnson-U/mg) a linear decline of O.D. (deltaA/min) between 5 and 50 mug bromelain/ml is observed. Other proteases as well as protease inhibitors may be analyzed by the same method also.
D001963:Bromelains / Q000032:analysis*; D007700:Kinetics; D008722:Methods; D010447:Peptide Hydrolases / Q000032:analysis; D010944:Plants / Q000201:enzymology*
D001963:Bromelains; D010447:Peptide Hydrolases
Automatisch-kinetische Bestimmung von pflanzlichen Enzymaktivitäten und Inhaltsstoffen. 3. Bestimmung von Bromelain