title: An ultrastructural analysis of chick embryonic skin development in vitro.
abstract: Behaviour of 6-day chick embryo thigh skin in vitro in two different nutrients was studied electron microscopically. In explants supported with chicken serum containing medium epithelium keratinized faster than and in a similar way to that in ovo, except for the absence of corpuscola cribriformia in pericytes. It did not in explants supported with chick embryo extract containing medium, but underwent a noticeable ultrastructural evolution, mainly of granular reticulum and Golgi complexes. In the two series of cultures mesenchyme presented the same ultrastructural characteristics, especially as far as collagen fibers were concerned. The above data rule out the suggested regulatory role of collagen fibers in epidermal differentiation and support a possible epidermal two-step differentiative process. They are discussed in relation to the general mechanisms implicated in skin evolution.
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D002642:Chick Embryo; D003470:Culture Media; D046508:Culture Techniques; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D012867:Skin / Q000196:embryology / Q000648:ultrastructure*
chemical_list: D003470:Culture Media