A study on the receptor for a mycobacteriophage : phage phlei.
From Mycobacterium phlei, glycolipid fractions have been isolated which inactivate phage Phlei. On the basis of the characteristics of the inactivation (specificity, kinetics, requirement for Ca++) typical of the phage-host cell system, it was concluded that these fractions contain the receptor sites for phage Phlei ; this conclusion was supported by electron microscopic studies. All the active fractions contain four kinds of components : fatty acids, glycerol, sugars (D-lyxose, 6-0-methyl-D-glucose, and low amounts of glucose and mannose), and water-soluble acids. These acids are isolated by degradation of the receptor fractions as oxalic and pyruvic acids. Variations of the ratio oxalic acid/pyruvic acid according to the mode of degradation and the absence of the peak characteristic of the protons of a pyruvic acid residue in the NMR spectrum, suggest that these acids might arise from the splitting of oxaloacetic acid. A tentative structure of the receptor is proposed, in many monoglycerides are linked through keto-acid to a polysaccharide core.
D001665:Binding Sites / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D006017:Glycolipids / Q000302:isolation & purification*; D006601:Hexoses / Q000032:analysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009160:Mycobacteriophages / Q000648:ultrastructure; D009161:Mycobacterium / Q000032:analysis*; D009168:Mycobacterium phlei / Q000032:analysis*; D010429:Pentoses / Q000032:analysis
D005227:Fatty Acids; D006017:Glycolipids; D006601:Hexoses; D010429:Pentoses; D005990:Glycerol; D002118:Calcium