Analysis of benzoylecgonine in urine by high-performance liquid chromatography and gas chromatography-mass spectrometry.
Benzoylecgonine, a polar metabolite of cocaine, was extracted and purified from urine using a reversed-phase high-performance liquid chromatographic column. The portion of the eluent corresponding to elution of the drug was collected, derivatized, and quantified using gas chromatography-mass spectrometry with selected ion monitoring. Using this procedure, analysis of 1 ng/ml of benzoylecgonine in urine can be achieved.
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives* / Q000652:urine*; D013058:Mass Spectrometry; D008832:Microchemistry; D012997:Solvents
D012997:Solvents; D003042:Cocaine