Concentrations of three hapten-binding immunoglobulins in pooled normal human serum.
The amount of natural anti-DNP-cap, anti-NIP-cap or anti-ABA-TYR in human IgM was measured by using immunoabsorbents and hapten elution. We found that 1.0% of pooled human IgM is specific for DNP-cap, 0.2% for NIP-cap and 0.02% for ABA-TYR. Haptens NIP and DNP were found to be cross-reactive when tested with natural antibodies even though they were not cross-reactive when tested with immune antibodies.
D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity; D001391:Azo Compounds / Q000276:immunology*; D001666:Binding Sites, Antibody; D004140:Dinitrophenols / Q000276:immunology*; D006241:Haptens; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism*; D010132:p-Azobenzenearsonate / Q000276:immunology*
D000906:Antibodies; D001391:Azo Compounds; D004140:Dinitrophenols; D006241:Haptens; D007075:Immunoglobulin M; D010132:p-Azobenzenearsonate