A method to measure the adsorption of bile salts to vegetable fiber of differing water holding capacity.
Dietary vegetable fibers have physico-chemical properties which may influence their role in nutrition. A method is described which measures bile acid adsorption to fibers of differing water holding capacity. Such absorption studies are complicated by the water held in the fiber matrix which includes bile acid in solution. The entrapped solution gives some apparent adsorption but this can be removed by washing the fibers with water. This method differentiates between strong adsorption and reversible adsorption. This enables dietary vegetable fibers to be classified according to their bile acid absorption capacity which may be useful in selecting fibers for clinical trials.
D000327:Adsorption; D001647:Bile Acids and Salts; D055598:Chemical Phenomena; D002621:Chemistry; D003840:Deoxycholic Acid; D006000:Glycocholic Acid; D008722:Methods; D009747:Nutritional Physiological Phenomena; D013045:Species Specificity; D014908:Triticum; D014675:Vegetables; D014867:Water
D001647:Bile Acids and Salts; D003840:Deoxycholic Acid; D014867:Water; D006000:Glycocholic Acid