Polyamines in bacteriophage phi W-14 and in phi W-14-infected Pseudomonas acidovorans.
Bacteriophage phi W-14 is sensitive to osmotic shock. It contain sufficient free putrescine, 2-hydroxyputrescine and spermidine to neutralize about 15% of the DNA phosphates. The alpha-putrescinylthymine residues of the DNA could neutralize a further 25% of the phosphates. Label is transferred from ornithine to the alpha-putrescinyl residues of phi W-14 DNA. The rates of polyamine synthesis in Pseudomonas acidovorans are increased by phi W-14 infection.
D001435:Bacteriophages / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D009995:Osmosis; D010710:Phosphates / Q000032:analysis; D011073:Polyamines / Q000032:analysis* / Q000096:biosynthesis; D011549:Pseudomonas / Q000378:metabolism*; D011700:Putrescine / Q000032:analysis; D013095:Spermidine / Q000032:analysis
D004279:DNA, Viral; D010710:Phosphates; D011073:Polyamines; D013095:Spermidine; D011700:Putrescine