Two complementary strand-specific termination sites for adenovirus DNA replication.
Adenovirus type 2 DNA, specifically labeled at the termini for DNA replication, was prepared by isolation of viral DNA molecules which were completed during short pulses with 3H-thymidine. The distribution of radioactivity in the two complementary strands at the termini for DNA replication was determined by liquid phase hybridization and gelelectrophoresis. At the right-hand terminus, nearly all radioactivity was found in the viral h strand, whereas at the left-hand terminus, most radioactivity was confined to the viral l strand. The results suggest that both molecular ends serve as origins and termini for replication of adenovirus type 2 DNA.
D000256:Adenoviridae / Q000378:metabolism*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D004586:Electrophoresis; D008954:Models, Biological; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D013936:Thymidine / Q000032:analysis; D014779:Virus Replication
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D013936:Thymidine