Reduction by cycloheximide of lysosomal proteolytic enzyme activity and rate of protein degradation in organ-cultured hearts.
Cycloheximide, an agent whose primary action is inhibition of protein synthesis, also causes a decrease in the rate of protein degradation in cultured fetal mouse hearts. This is associated with marked decreases in the activities of cathespin D and other lysosomal hydrolases. It is suggested that reduced lysosomal proteolytic capacity may contribute to cycloheximide-induced inhibition of protein degradation.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002403:Cathepsins / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology*; D005260:Female; D005318:Fetal Heart / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology*; D051379:Mice; D009924:Organ Culture Techniques; D010447:Peptide Hydrolases / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D011247:Pregnancy
D010649:Phenylalanine; D003513:Cycloheximide; D003402:Creatine Kinase; D000135:Acid Phosphatase; D000118:Acetylglucosaminidase; D002403:Cathepsins; D010447:Peptide Hydrolases