title: Oxidative phosphorylation in brown adipose tissue mitochondria from rats kept under normal environmental conditions.
abstract: Based on criteria such as the ADP/O ratio and respiratory control by ADP, the energy-coupling efficiency of brown adipose tissue mitochondria isolated from rats kept under normal environmental conditions for a long time decreased remarkably. The presence of bovine serum albumin, GTP, or ATP plus carnitine in the reaction medium markedly increased the efficiency of oxidative phosphorylation of brown adipose tissue mitochondria. Pre-treatment of brown adipose tissue mitochondria with 2% bovine serum albumin, GTP, or ATP plus carnitine caused a decrease in the amount of free fatty acids bound to the mitochondria from 13.1 to 7.0, 9.0, or 8.2 mug per mg protein, respectively. Removal of the free fatty acids by means of these pre-treatments resulted in restoration of efficient oxidative phosphorylation; there was a correlation between the amount of free fatty acids removed and the degree of recovery in the respiratory control ratio. The elimination of only a fraction of the free fatty acids, as little as 4 mug per mg protein, was sufficient to ensure respiratory control by ADP. It appears that the free fatty acids which lie mainly outside the inner mitochondrial membrane are responsible for the decrease in the efficiency of oxidative phosphorylation in brown adipose tissue mitochondria isolated from rats kept under normal environmental conditions.
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D002331:Carnitine / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005260:Female; D006160:Guanosine Triphosphate / Q000494:pharmacology; D007656:Ketoglutaric Acids / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology
chemical_list: D005230:Fatty Acids, Nonesterified; D007656:Ketoglutaric Acids; D012710:Serum Albumin, Bovine; D000244:Adenosine Diphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D002331:Carnitine