High-performance liquid chromatographic separation of C-15-epimers of 15-methylprostaglandin E2 methyl ester and 15-methylprostaglandin E2.
A high-performance liquid chromatographic system that permits quantitation of 15-methylprostaglandin E2 and its methyl ester is described. Separation from the corresponding C-15 epimers is effected by adsorption chromatography on microparticulate silica with refractive index detection.
D002851:Chromatography, High Pressure Liquid; D007536:Isomerism; D008722:Methods; D011458:Prostaglandins E / Q000302:isolation & purification*
D011458:Prostaglandins E