Measurement of 25-hydroxycholecalciferol in the bovine.
Bovine plasma 25-hydroxycholecalciferol was estimated by rapid protein binding assay. Serum-binding proteins in the assay were from rats deficient in vitamin D and from normal rats; no differences existed in standard curves. Nanograms 25-hydroxycholecalciferol in eight cows averaged 45.5 +/- 12.2 ml of plasma. Results from the rapid protein binding assay compared favorably (greater than 10% deviation) with values from a 25-hydroxycholecalciferol assay involving high pressure liquid chromatography.
D000818:Animals; D002417:Cattle; D005260:Female; D006887:Hydroxycholecalciferols / Q000097:blood*; D008297:Male; D011485:Protein Binding; D051381:Rats; D012712:Serum Globulins / Q000378:metabolism; D014808:Vitamin D Deficiency / Q000097:blood
D006887:Hydroxycholecalciferols; D012712:Serum Globulins