Preparation of concentrated platelet suspensions by dehydration against polyethyleneglycol 20,000.
Gel filtered platelet suspensions were concentrated by repeated dehydration against polyethyleneglycol 20,000 followed by dialysis against slightly hypotonic buffer. This method increased the platelet concentration 2-3 times with a recovery of 80-100%. The final cell suspension closely resembled the original platelet rich plasma as tested by a number of platelet tests.
D000244:Adenosine Diphosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001772:Blood Cell Count; D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure; D002469:Cell Separation; D002850:Chromatography, Gel / Q000379:methods*; D006019:Glycolysis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007773:Lactates / Q000097:blood; D010101:Oxygen Consumption; D010977:Platelet Factor 3 / Q000032:analysis; D011092:Polyethylene Glycols; D012701:Serotonin / Q000097:blood; D013535:Suspensions
D007773:Lactates; D013535:Suspensions; D012701:Serotonin; D010977:Platelet Factor 3; D011092:Polyethylene Glycols; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase