title: [High-performance liquid chromatography of penimocycline (author's transl)].
abstract: Penimocycline is an antibiotic obtained by Mannich reaction between tetracycline and ampicilline. Separation of penimocycline from tetracyclines and other impurities has been studied by high-performance liquid chromatography. The most effective method is liquid-liquid partition on a Micropak CH column (non-polar hydrocarbon bonded on porous silica microparticles) and gradient elution with water-methanol, 0.02 M phosphate buffer (pH 7.6) and 1 mM EDTA. Some results on hydrolysis of penimocycline are given.
mesh_terms: D000667:Ampicillin / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D000432:Methanol; D013754:Tetracyclines / Q000032:analysis*; D014867:Water
chemical_list: D013754:Tetracyclines; D014867:Water; C010998:penimocycline; D000667:Ampicillin; D000432:Methanol
vernacular_title: Etude de la pénimocycline par chromatographie en phase liquide à haute performance