Competitive protein binding assay of oestradiol-17 beta and oestrone using uterine cytosol prepared from HCG-treated rabbits.
Uterine cytosol was prepared from rabbits after treatment with human chorionic gonadotrophin (HCG) and used for the determination of oestradiol-17 beta and oestrone by competitive protein binding (CPB) assay. The so obtained uterine cytosol gave higher percentage of binding and proved to be more stable when stored at --20 degrees C than cytosol obtained from the pregnant uterus.
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D004958:Estradiol / Q000097:blood*; D004970:Estrone / Q000097:blood*; D005260:Female; D006801:Humans; D011247:Pregnancy; D011555:Pseudopregnancy; D011817:Rabbits; D011869:Radioligand Assay / Q000379:methods*; D014599:Uterus
D006063:Chorionic Gonadotropin; D004970:Estrone; D004958:Estradiol