Micromonospora-produced sisomicin components.
A sisomicin fermentation carried out in the presence of (methyl-14C)-L-methionine resulted in a crude mixture, composed of methyl-14C-labeled sisomicin as a major component; and two 4''-C-desmethylsisomicin (66-40B and 66-40D) isomer-like components, an unidentified component and a gentamicin A-like antibiotic as minor components. When (methyl-14C)-L-methionine was added in an early stage of the fermentation (24 hours), incorporation of methyl-14C-label into polar components (e.g., gentamicin A-like antibiotic) preceded that into sisomicin. Chromatographic evidence for the bioconversion of (methyl-14C)-gentamicin A to a radioactive sisomicin-like product (possibly (3''-N-methyl-14C)-sisomicin) was seen, when a Micromonospora blocked mutant was incubated in the presence of the former antibiotic.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001711:Biotransformation; D005285:Fermentation; D005839:Gentamicins / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D008847:Micromonospora / Q000378:metabolism*; D012853:Sisomicin / Q000096:biosynthesis*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D008715:Methionine; D012853:Sisomicin