Binding of aminoacyl-tRNA to rat liver ribosomal proteins.
Rat liver ribosome treatment with ethanol and 1 M NH4Cl releases some 31-33 ribosomal proteins. This split protein fraction binds Phe-tRNA, Ac-Phe-tRNA, Met-tRNAM and f-Met-tRNAF in the absence of K+ and Mg++ ions. When the split protein fraction is passed through Sephadex G-100 only six proteins are retained in the column: S10, S14, S15, S19, L35, and L36. The aminoacyl-tRNA binding activity of this protein fraction retained in the Sephadex G-100 column is similar to that of the total split protein fraction, suggesting that the above six proteins, or only some of them, are involved in the binding reaction.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001665:Binding Sites; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008715:Methionine; D010649:Phenylalanine; D011072:Poly U / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D011485:Protein Binding; D012343:RNA, Transfer / Q000378:metabolism; D051381:Rats; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism
D000900:Anti-Bacterial Agents; D012269:Ribosomal Proteins; D011072:Poly U; D010649:Phenylalanine; D012343:RNA, Transfer; D008715:Methionine; D008274:Magnesium; D011188:Potassium