[Micromethod of diazepam determination in the blood by gas-liquid chromatography. Application in neonatal pharmacology].
We propose a micromethod of diazepam determination on 0,1 ml of whole blood. The extraction is made with n-heptane for the isolation of diazepam in presence of principal metabolites. For the usual concentrations, the precision is about 3 per cent; at the detection limite, 0,05 mug/ml, it is about 20 per cent. With this method we studied the pharmacokinetics of diazepam in neonate with convulsive diseases in comparison with electroencephalogram.
D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D003975:Diazepam / Q000097:blood* / Q000378:metabolism / Q000627:therapeutic use; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000188:drug therapy*; D007700:Kinetics; D012640:Seizures / Q000188:drug therapy*
D003975:Diazepam
Microméthode de dosage du diazépam dans le sang par chromatographie gaz-liquide. Application en pharmacologie néonnatale