[Determination of phosphonic acids in ruminal fluid].
A technique has been worked out for determining amino alkyl phosphonic acids in ruminal fluid by using methods of hydrolysis, partition of the phosphonic acid on a cation exchange column and ashing of the eluate. After evaporation to total dryness the phosphonic acids were determined as phosphates. The amounts of acid found in the ruminal fluid of goats varied between 0.4 and 1.1 muMol/ml. In view of the fact that these acids are microbial compounds it will appear that any change in the amount of compounds produced in the rumen would indicate changes in the concentration of the microbial mass in the rumen.
D000818:Animals; D002417:Cattle; D002845:Chromatography; D004063:Digestion; D006868:Hydrolysis; D063065:Organophosphonates / Q000378:metabolism*; D012417:Rumen / Q000378:metabolism / Q000502:physiology*
D063065:Organophosphonates
Bestimmung von Phosphonsäuren in der Pansenflüssigkeit