Selective histochemical localization of polysaccharides in tissue sections oxidized by acetic anhydride in dimethyl sulfoxide.
Oxidation of tissue sections by 25-30% (v/v) acetic anhydride (AA) in dimethyl sulfoxide (DMSO) resulted in facile induction of tissue carbonyls readily localized with Schiff's reagent and o-dianisidine but not with the 3-hydroxy-2-naphthoic acid hydrazide-tetraazotized diorthoanisidine method. Carbonyls generated by AA-DMSO oxidation were confined predomintly to substrates containing pyranosides. Oxidized furanosides, as represented by deoxyribonucleic acid and ribonucleic acid, gave only a residual color reaction. The AA-DMSO method possesses an advantage in that the oxidation of tissue polysaccharides does not proceed beyond the formation of carbonyly and is particularly suited for use after formol fixation.
D000085:Acetates; D000812:Anhydrides; D000818:Animals; D002356:Cartilage / Q000648:ultrastructure; D006224:Cricetinae; D004121:Dimethyl Sulfoxide; D006651:Histocytochemistry; D008099:Liver / Q000648:ultrastructure; D008722:Methods; D009928:Organ Specificity; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000032:analysis*; D013552:Swine
D000085:Acetates; D000812:Anhydrides; D011134:Polysaccharides; D004121:Dimethyl Sulfoxide