Preparation and properties of a poly A resin and its use in the isolation of naturally occurring poly U sequences.
A method for the covalent attachment of poly A, as well as other nucleic acids and nucleosides, to a methylene dianiline derivative of starch is described. The properties of this poly A resin and its use for the recovery of poly U sequences from both nuclear and cytoplasmic extracts of HeLa cells is described.
D002467:Cell Nucleus / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods; D003593:Cytoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000032:analysis; D009693:Nucleic Acid Hybridization; D011061:Poly A; D011072:Poly U / Q000302:isolation & purification*; D012334:RNA, Neoplasm; D012116:Resins, Plant; D012260:Ribonucleases; D013347:Subcellular Fractions / Q000032:analysis
D012334:RNA, Neoplasm; D012116:Resins, Plant; D011061:Poly A; D011072:Poly U; D012260:Ribonucleases