title: Gas-liquid chromatographic determination of chlorphoxim residues in water and fish by in-block methylation.
abstract: A gas-liquid chromatographic method has been developed for the analysis of residues of chlorphoxim, 2-chloro-alpha ((diethoxyphosphinothioyl)oxy)imino)-benzeneacetonitrile, in water and fish. The method is based on the in-block methylation of chlorphoxim with 0.01M trimethylanilinium hydroxide in methanol. The derivative, O,O-diethyl O-methyl phosphorothioate, was determined quantitatively by using a flame photometric detector specific for phosphorus. The in-block reaction is 70% efficient. Water samples were extracted with hexane; fish were extracted with methylene chloride and cleaned up on an acetonitrile-hexane partition column. Recoveries from water and fish samples spiked with chlorphoxim averaged 86.3 and 80.4%, respectively. Limits of detection were 10.0 ppb for 5 g samples of fish and 0.10 ppb for 300 ml water samples.
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D002853:Chromatography, Liquid; D005399:Fishes / Q000378:metabolism*; D007306:Insecticides / Q000032:analysis*; D008722:Methods; D008745:Methylation; D009946:Organothiophosphorus Compounds; D010573:Pesticide Residues / Q000032:analysis*; D014873:Water Pollutants / Q000032:analysis*
chemical_list: D007306:Insecticides; D009946:Organothiophosphorus Compounds; D010573:Pesticide Residues; D014873:Water Pollutants; C004159:chlorphoxim