title: Lesions in post-ribosomal supernatant fractions associated with loss of viability in pea (Pisum arvense) seed.
abstract: RNA synthesis and protein synthesis in viable pea embryonic axis tissue commences during the first hour of water imbibition whilst DNA synthesis commences after 8 h of imbibition. Neither DNA synthesis nor protein synthesis could be detected in non-viable axis tissue during the first 24 h of imbibition but some RNA synthesis is detectable during this period. Both post-ribosomal supernatant and ribosomal fractions from imbibed non-viable embryonic axis tissue were impaired in their ability to support polyphenylalanine synthesis in a cell-free protein-synthesising system, yet the same fractions isolated from unimbibed non-viable axis tissue were as efficient as equivalent fractions from unimbibed viable axis tissue in the support of polyphenylalanine synthesis in the cell-free system. A major lesion in elongation factor 1 activity and additional lesions in elongation factor 2 and phenylalanyl-tRNA synthetase activities were detected in the post-ribosomal supernatants isolated from non-viable embryonic axis tissue.
mesh_terms: D003600:Cytosol / Q000378:metabolism; D004261:DNA Replication; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects; D010445:Peptide Elongation Factors; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis; D011072:Poly U; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism; D012639:Seeds / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014158:Transcription, Genetic; D014529:Uridine / Q000378:metabolism
chemical_list: D010445:Peptide Elongation Factors; D010940:Plant Proteins; D011072:Poly U; D010652:Phenylalanine-tRNA Ligase; D007930:Leucine; D008274:Magnesium; D013936:Thymidine; D014529:Uridine