Phenol extraction of 28 S ribosomal RNA from rat liver cytoplasm.
A simple and reproducible phenol method for the isolation of 28 S ribosomal RNA from rat liver cytoplasm, free from poly(A)+/-RNA is described. The procedure is based on the observation that at lower pH of the homogenate (pH 5.5) 28 S ribosomal RNA is extracted, while 18 S ribosomal RNA remains in the interphase layer.
D000818:Animals; D003593:Cytoplasm / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D008099:Liver / Q000032:analysis*; D008722:Methods; D010636:Phenols; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D051381:Rats
D010636:Phenols; D012335:RNA, Ribosomal