Immunoelectrophoretic determination of brain-specific antigens in bulk-prepared neuronal and glial cells.
The relative amounts of brain-specific antigens, S-100, glial fibrillary acidic protein (GFA), 14.3.2, synaptin C1, D1, D2 and D3 were determined by crossed immunoelectrophoresis in bulk-prepared neuronal, glial and synaptosomal fractions from 35-day-old rat brains. Little enrichment was ontained for any antigen in the neuronal perikaryal fraction. The glial fraction showed a 3.5-fold enrichment in GFA but had levels similar to whole brain with respect to S-100. Synaptosomes were somewhat enriched in the synaptin C1 protein, but did not differ markedly from whole brain with respect to the most antigens. The extent  of cross-contamination in the various fractions could be judged only tentatively, partly due to differences in extractability of the proteins.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001921:Brain / Q000276:immunology* / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000032:analysis; D009457:Neuroglia / Q000276:immunology; D009474:Neurons / Q000276:immunology; D051381:Rats; D013574:Synaptosomes / Q000276:immunology
D000941:Antigens; D009419:Nerve Tissue Proteins