title: Prostaglandins inhibit testosterone secretion by mouse testes in vitro.
abstract: Production of testosterone (T) by decapsulated mouse tests in vitro was significantly inhibited by adding prostaglandin (PG) A1, PGA2 or PGE1 to the incubation medium. Prostaglandin A1 at a concentration of 10(-6)M inhibited T production in this system both in the presence of moderate amounts of hCG (12.5 or 25.0 mIU/ml), and in the absence of gonadotropins. However, in the presence of very high levels of hCG (125.0 mIU/ml), all PGs tested appeared to have had a slight potentiating effect on T production when added in concentrations ranging from 10(-7) to 10(-5)M, and the inhibition of T accumulation in the medium was consistently observed only when the concentration of PGs was increased to 10(-3)M. These results suggest that a direct effect of PGs on testicular steroidogenesis may account for, or contributes to, the decrease in peripheral T levels observed after administration of PGs in vivo.
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D011454:Prostaglandins A / Q000494:pharmacology; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D011454:Prostaglandins A; D013739:Testosterone