Colorimetric determination of piperazine.
A sensitive method for the quantitative determination of piperazine is described. The method is precise and responds linearly from 25 g to 500 mug and above of the material. The procedure is based on the formation of a complex of piperazine with reineckate in neutral or acid medium. The complex can be separated by centrifugation. It is then dissolved in acetone and estimated at 530 nm in a colorimeter. Piperazine present in trichloroacetic acid extractrs of biological samples can also be estimated by this method.
D000818:Animals; D001200:Ascaris / Q000032:analysis; D002857:Chromium; D003124:Colorimetry / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D008099:Liver / Q000032:analysis; D010879:Piperazines / Q000032:analysis*; D000644:Quaternary Ammonium Compounds; D013861:Thiocyanates
D007202:Indicators and Reagents; D010879:Piperazines; D000644:Quaternary Ammonium Compounds; D013861:Thiocyanates; D002857:Chromium; C001818:Reinecke Salt