Hormonal and nutritional effects on the binding of 125I-labeled anti-serine dehydratase Fab' to rat tissue polysomes.
With 125I-labeled Fab' specific for rat liver serine dehydratase it has been possible to localize polyribosomes synthesizing the enzyme under several different environmental conditions. Evidence is presented to show that, following the administration of amino acids in vivo, the relative synthetic capabilities of free and membrane-bound polyribosomes synthesizing serine dehydratase vary with time. Early during the period of induction of the enzyme by administration of amino acids or by feeding a high protein diet the majority of the newly synthesized enzyme is derived from membrane-bound polyribosomes. Later in the induction process an increasing proportion of the enzyme is synthesized by the free polyribosomes. Subcellular localization studies clearly show that serine dehydratase is synthesized by both subclasses of hepatic membrane-bound polyribosomes, the loose and tight membrane-bound polyribosomes, as well as by the free polyribosomes. It was found that the membrane-bound polyribosomes are the preferential sites of synthesis of the majority of serine dehydratase molecules in the Morris hepatomas 5123C and 7800. It is concluded that the synthesis of the enzyme, serine dehydratase, in rat liver is not discretely compartmentalized in either class of free or membrane-bound polyribosomes. Rather, the relative proportions of the serine dehydratase synthesizing polyribosomes within these two classes of polyribosomes can vary depending on the metabolic and physiologic state of the liver cell.
D000818:Animals; D001666:Binding Sites, Antibody; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D007467:Iodoproteins; D007668:Kidney / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D012695:L-Serine Dehydratase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D011132:Polyribosomes / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D051381:Rats
D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D007467:Iodoproteins; D012695:L-Serine Dehydratase