Fluorescence study of N-(3-pyrene)maleimide conjugated to rabbit skeletal F-actin and plasmodium actin polymers.
A fluorescent probe N-(3-pyrene)maleimide was conjugated to rabbit skeletal F-actin at the site of most reactive sulfhydryl group (Cys-373). Its fluorescence anisotropy decay showed a single correlation time of 560 ns at 25 degrees C, which is in a very good agreement with the correlation time of the dansyl-L-cysteine group conjugated to the same site of F-actin reported very recently [Wahl, Ph., Mihashi, K, and Auchet, J-C. (1975) FEBS Lett. 8, 164-167]. Actin from plasmodia of myxomycates, Physarum polycepharum, was also conjugated with N-(3-pyrene) maleimide and the fluorescence anisotropy was compared with rabbit skeletal F-actin using the classical steady excitation method. It was found that the internal mobility of the magnesium polymer of plasmodium actin is remarkably larger than both plasmodium F-actin and rabbit skeletal F-actin.
D000199:Actins; D000818:Animals; D001665:Binding Sites; D003201:Computers; D004735:Energy Transfer; D005456:Fluorescent Dyes; D007700:Kinetics; D008301:Maleimides; D008433:Mathematics; D009132:Muscles; D010961:Plasmodium; D011485:Protein Binding; D011487:Protein Conformation; D011721:Pyrenes; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D000199:Actins; D005456:Fluorescent Dyes; D008301:Maleimides; D011721:Pyrenes; C003944:N-(3-pyrene)maleimide