[Large scale purification of the acetylcholinesterase from bovine caudate nucleus by affinity chromatography (author's transl)].
The acetylcholinesterase from bovine caudate nucleus was solubilized with 0.6-0.8% Triton X-100. It was purified and freed from Triton X-100 by means of a two-fold affinity chromatography procedure. A simple three-step synthesis of the inhibitor with attached spacer arm used in affinity chromatography is described. Starting from 3 kg of caudate nucleus, nearly 11 mg of the purified acetylcholinesterase with a spec. act of 4250 U/mg (13 500-fold purification) could be obtained in an over-all yield of 49%. On gel electrophoresis the enzyme produces several enzymatically active glycoproteinbands of oligomeres. On sodium dodecylsulfate gel electrophoresis in the presence of a disulfide-reducing agent it yields a molecular weight of 72000 +/- 2000 for the smallest subunit. In the absence of a disulfide-reducing agent a band for a dimer besides the band for the monomer could be detected and a molecular weight of 142000 +/- 3000 was estimated for this species.
D000110:Acetylcholinesterase / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002421:Caudate Nucleus / Q000201:enzymology*; D002800:Cholinesterase Inhibitors / Q000138:chemical synthesis; D002846:Chromatography, Affinity; D003720:Densitometry; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D008722:Methods; D008970:Molecular Weight; D012967:Sodium Dodecyl Sulfate
D002800:Cholinesterase Inhibitors; D012967:Sodium Dodecyl Sulfate; D000110:Acetylcholinesterase
Affinitätschromatographische Reinigung der Acetylcholinesterase aus Nucleus Caudatus vom Rind im Grossmassstab