Effects of N-methyl-N-nitrosourethane on DNA synthesis in the guinea pig pancreas.
The effects of N-methyl-N-nitrosourethane (NMUT) on pancreatic DNA synthesis were investigated at sequential intervals following gavage of Hartley guinea pigs with a single dose of 30 mg/kg. There was a highly significant stimulation of DNA synthesis, as evidenced by increased incorporation of [3H] methyl-thymidine ([3H]TdR), throughout the whole pancreas and particularly in the duodenal segment, at 4 h following NMUT administration, thereafter, DNA synthesis declined sharply up to 24 h, and then recovered gradually to control levels from 24--96 h. DNA synthesis stimulated by NMUT was suppressed by hydroxyurea (HU), and hence is likely to represent replicative, rather than repair, synthesis.
D000818:Animals; D004261:DNA Replication / Q000187:drug effects*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009605:Nitrosomethylurethane / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014520:Urethane / Q000031:analogs & derivatives*
D014520:Urethane; D009605:Nitrosomethylurethane