[Muscle metabolism of glucose, lactate, free fatty acids, and glycerol at rest and during electrically stimulated exercise in the perfused rat hind limb (author's transl)].
The influence of insulin on muscle metabolism at rest and during electrically stimulated exercise was examined in the perfused rat hind limb. Basal glucose uptake of muscle tissue was enhanced threefold both by muscle contraction an by addition of physiological amounts of insulin. A further increase in glucose uptake was seen, when muscle was exercised in the presence of insulin. Lactate production rose 7 - 8fold by contractions. Insulin had no additional effect on lactate production during exercise. The work-induced lactate output exceeded glucose uptake, corresponding to a significantly reduced glycogen content after exercise. Basal lipolysis was not stimulated by muscle contractions. At rest the addition of both corticosterone and thyroxine did not increase the basal lipolysis. Yet, glycerol release rose twofold by the addition of serum to the perfusion medium. It is assumed that in the perfused rat hind limb glycogenolysis will promptly be stimulated by muscle contractions, whereas lipolysis can not be enhanced by exercise alone.
D000818:Animals; D004558:Electric Stimulation; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006614:Hindlimb; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007773:Lactates / Q000096:biosynthesis; D050356:Lipid Metabolism; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010477:Perfusion; D005082:Physical Exertion; D051381:Rats
D005230:Fatty Acids, Nonesterified; D007328:Insulin; D007773:Lactates; D006003:Glycogen; D005947:Glucose; D005990:Glycerol
Kohlenhydrat- und Fettstoffwechsel der Skelettmuskulatur unter Ruhebedingungen und bei kurzfristiger submaximaler Arbeit am Modell des perfundierten Hinterbeins der Ratte