Methylation pattern of genomic RNA from Moloney murine leukemia virus.
32P- and methyl-3H-labeled 70S Moloney murine leukemia virus RNA was purified from virions produced in Moloney murine leukemia virus-infected mouse embryo cells. Primer-free RNA subunits obtained by heat treatment and zonal centrifugation were digested with RNase T2, and methylated oligonucleotides were chromatographed on DEAE-Sephadex in 7 M urea. Approximately one molecule of RNase T2-stable oligonucleotide (-5 charge) was isolated per subunit. Structural analysis indicated that the sequence of the oligonucleotide is m7GpppGmpCp. Analysis of the mononucleotide fraction isolated by DEAE-Sephadex chromatography of the RNase T2 digest identified 15 to 23 internal N6-methyladenylic acid molecules per subunit.
D000249:Adenosine Monophosphate / Q000032:analysis; D001483:Base Sequence; D008745:Methylation; D008979:Moloney murine leukemia virus / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012260:Ribonucleases / Q000378:metabolism
D009841:Oligonucleotides; D012367:RNA, Viral; D000249:Adenosine Monophosphate; D012260:Ribonucleases