Sister chromatid differentiation and exchanges in adult mudminnows (Umbra limi) after in vivo exposure to 5-bromodeoxyuridine.
An in vivo system for the detection of sister chromatid exchange (SCE) in the central mudminnow, Umbra limi, is presented. Sister chromatid differential (SCD) and SCE were demonstrated by fluorescent and Giemsa procedures 5 to 6 days after the fish were injected with 500 mug/g of BrdU. The exchange rate was found to be 2.64 SCEs metaphase in the intestines and 2.42 SCEs/metaphase in the gills. SCE analysis in U. limi should be a useful tool for measuring the mutagenicity of water-borne chemicals.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002842:Chromatids / Q000187:drug effects*; D003434:Crossing Over, Genetic / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005399:Fishes; D008954:Models, Biological; D009153:Mutagens; D014874:Water Pollutants, Chemical / Q000009:adverse effects
D009153:Mutagens; D014874:Water Pollutants, Chemical; D001973:Bromodeoxyuridine