Selected ion monitoring assay for the bronchodilator pirbuterol.
A selected ion monitoring assay has been developed to measure plasma levels of the bronchodilator pirbuterol in animals and man. Pirbuterol is extracted from plasma with acetone + potassium carbonate and after further purification is converted to its trimethylacetyl derivative. With the use of the stable isotope labeled internal standard, pirbuterol-d9, precisions of 10% and 6% were achieved at plasma concentrations of 1 and 20 ng ml-1, respectively. The synthesis of the deuterium labeled internal standard is described.
D001993:Bronchodilator Agents / Q000097:blood* / Q000138:chemical synthesis; D002082:Butylamines; D002849:Chromatography, Gas; D003903:Deuterium; D004147:Dioxins; D004983:Ethanolamines / Q000097:blood / Q000138:chemical synthesis; D013058:Mass Spectrometry; D008991:Monitoring, Physiologic; D011725:Pyridines / Q000097:blood* / Q000138:chemical synthesis
D001993:Bronchodilator Agents; D002082:Butylamines; D004147:Dioxins; D004983:Ethanolamines; D011725:Pyridines; D003903:Deuterium; C009118:pirbuterol