Peptido polysaccharide antigens from Trichophyton mentagrophytes var. granulosum.
Two highly purified peptido polysaccharide antigens have been isolated from surface-grown cultures of Trichophyton mentagrophytes var. granulosum. Trichloroacetic acid extraction and ethanol precipitation yielded a mixture containing high-molecular-weight components which were first separated on Sephadex G-200. Subsequent fractionation by ion-exchange chromatography on DE-52-cellulose (borate form) yielded the two peptido polysaccharides. Both of the peptido polysaccharides reacted with rabbit antiserum to T. mentagrophytes var. granulosum. The two peptido polysaccharides contain 73.2% hexoses (mannose-galactose-glucose, 7.5:0.7:1), 8.6% amino acids and 1.8% amino sugars and 77.4% hexoses (mannose-galactose-glucose, 9:0.3:1), 6.2% amino acids, and 0.4% amino sugars, respectively. Each contains 16 different amino acids, threonine, proline, and serine predominating.
D000946:Antigens, Fungal / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004589:Electrophoresis, Disc; D005690:Galactose / Q000302:isolation & purification; D005947:Glucose / Q000302:isolation & purification; D007122:Immunoelectrophoresis; D008358:Mannose / Q000302:isolation & purification; D010455:Peptides / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D014249:Trichophyton / Q000276:immunology*
D000946:Antigens, Fungal; D010455:Peptides; D011134:Polysaccharides; D005947:Glucose; D008358:Mannose; D005690:Galactose