Reduction of acetylene by stationary cultures of free-living Rhizobium sp. under atmospheric oxygen levels.
The reduction of acetylene to ethylene by stationary (non-shaking) cultures of free-living rhizobia under atmospheric oxygen levels has been demonstrated. Under these conditions the development of the activity is inhibited by 10 mM NH4Cl and about 20% of oxygen is required for maximal activity. When the stationary cultures were shaken, oxygen concentrations of 1% and higher were found to be inhibitory. Specific activities of 20 and 40 nmol of acetylene reduced h-1 mg-1 protein were observed.
D000114:Acetylene / Q000378:metabolism*; D000643:Ammonium Chloride / Q000378:metabolism; D001274:Atmospheric Pressure; D001426:Bacterial Proteins / Q000096:biosynthesis; D005030:Ethylenes / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen; D012231:Rhizobium / Q000378:metabolism*
D001426:Bacterial Proteins; D005030:Ethylenes; D000643:Ammonium Chloride; D000114:Acetylene; D010100:Oxygen