Cultured fucosidosis fibroblasts: a simple technique demonstrating storage of tritiated-fucose labeled material.
Incorporation of tritiated fucose into the acid-soluble and acid-insoluble fractions of cultured fucosidosis and control fibroblast monolayers was determined over a 10-day period. Fucosidosis cells incorporated significantly more label into the acid-soluble, but not the acid-insoluble fraction, than control cells. The ratio of acid-insoluble radioactivity increased lineraly from 1 to 10 days for the fucosidosis cells, indicating the storage of low molecular weight partial degradation products.
D002239:Carbohydrate Metabolism, Inborn Errors / Q000378:metabolism*; D002478:Cells, Cultured; D004186:Disaccharidases / Q000172:deficiency*; D005347:Fibroblasts / Q000378:metabolism*; D005643:Fucose / Q000378:metabolism*; D006801:Humans; D014316:Tritium; D005644:alpha-L-Fucosidase / Q000172:deficiency*
D014316:Tritium; D005643:Fucose; D004186:Disaccharidases; D005644:alpha-L-Fucosidase