Incorporation of 3H-thymidine and 14C-amino acids into the ventral prostate after in vivo treatment with estradiol-3N-BIS(2-chloroethyl)carbamate-17 beta-phosphate (Estracyt) and its estrogen and cytostatic parts.
The in vivo effect of Estracyt (a nitrogen mustard linked to estradiol-17 beta-phosphate) on testosterone-stimulated incorporation of 3H-thymidine and 14C-amino acids into the ventral prostate of castrated rats has been investigated. A dose of 100 mg of Estracyt per kg of body weight injected intravenously daily for 3 days significantly reduced isotope incorporation. Equivalent amounts of the two main components of Estracyt, the nitrogen mustard and estradiol-17 beta-phosphate, were not able to mimic this Estracyt effect. Possible implications of this difference and the intracellular mechanism of action of Estracyt are discussed.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D004961:Estramustine / Q000494:pharmacology*; D008297:Male; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D051381:Rats; D013936:Thymidine / Q000378:metabolism*
D000596:Amino Acids; D009588:Nitrogen Mustard Compounds; D004961:Estramustine; D004958:Estradiol; D013936:Thymidine