Cell volume and permeability of oxygen-and glucose-deprived retina in vitro.
Rabbit retina was deprived of O(2) and glucose in vitro for up to four hours at 37 C. Intracellular volume was measured, using inulin as an extracellular marker. After a 30-minute latency, cells swelled rapidly to more than twice normal volume while extracellular volume was unchanged. Intracellular accumulation of water was not reversed by resupply of oxygen and glucose. Permeability to small molecules was assessed with mannitol. The ratio of mannitol space to inulin space averaged 1.0 in controls. This ratio remained 1.0 up to 30 minutes of deprivation, but increased to 1.2 by 60 minutes. Permeability to large molecules was assessed from the rate of loss of isotopically labeled cell protein into the medium. There was no difference between control and deprived retinas up to three hours.
D000818:Animals; D001834:Body Water; D002463:Cell Membrane Permeability; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D010101:Oxygen Consumption; D011817:Rabbits; D012160:Retina / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
D005947:Glucose