Diversity of sequences in total and polyadenylated nuclear RNA from Drosophila cells.
Complementary DNA was synthesized using polyadenylated nuclear RNA of cultured Drosophila cells as template. The kinetics of hybridization of this cDNA with nuclear RNA indicated that the complexity of this RNA population is five to ten times greater than that of cytoplasmic mRNA. The same difference in the fraction of DNA represented was obtained when nuclear and cytoplasmic RNA were hybridized with labeled unique sequence DNA. The fraction of the DNA sequences represented in total number of polyadenylated nuclear RNA is much higher than that represented in cytoplasmic RNA.
D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis*; D004330:Drosophila; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D011061:Poly A / Q000032:analysis; D012313:RNA / Q000302:isolation & purification
D011061:Poly A; D012313:RNA