Activities of glucose-6-phosphate, 6-phosphogluconate, and isocitrate dehydrogenases from Leishmania donovani cultivated at 25 and 37 C.
The activities of glucose-6-phosphate dehydrogenase (G-6-PD) (EC No. 1.1.1.49), 6-phosphogluconate dehydrogenase (PGD) (EC No. 1.1.1.44), and isocitrate dehydrogenase (ICD) (EC No. 1.1.1.42) from promastigotes of Leishmania donovani strain 3S grown at 25 C in modified Tobie's (mT) medium and from promastigotes of the 37 C-adapted substrain of this strain cultivated in the mT at 37 C were assayed at 25 and 37 C. At 25 C ICD from both the strain and the substrain had the highest, and PGD, the lowest activity; the activity of G-6-PD was intermediate, but much closer to that of ICD. Irrespective of the temperature of the assay, the activities of G-6-PD and ICD from the 37 C substrain were significantly higher than those of these enzymes from the parental strain; however, the activity of PGD from the 25 C strain was slightly higher than that of this dehydrogenase from the 37 C-adapted stock. No significant activity losses of G-6-PD and ICD from either the strain or the substrain were noted after incubation of the extracts in the presence of 0.25 M sucrose at 37 C for 2 hr. PGD was unstable in such extracts, but it could be rendered stable by the addition of 4 mM 6-phosphogluconate, G-6-PD was the least and ICD the most dependent on Mg2+ ions. In the 15-25 C range, the Q10 values of the enzymes from the 25 C strain were 2.83, 2.5 and 2.63 for G-6-PD, PGD, and ICD, respectively. These values for the respective enzymes in the 25-35 C range were 2.06, 1.67, and 1.62. The Q10 values of the enzymes from the 37 C substrain in the 15-25 C range were 2.06 for G-6-PD, 3.25 for PGD, and 2.77 for ICD; in the 25-35 C range, the corresponding values were 1.67, 1.46, and 1.83. Cultivation of the 37 C substrain at 25 C was accompanied by a drop in G-6-PD and ICD activities.
D000818:Animals; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D007891:Leishmania / Q000201:enzymology* / Q000254:growth & development; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism*; D013696:Temperature
D008345:Manganese; D007521:Isocitrate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D008274:Magnesium