Comparison of methods for separating proteins using bisalbuminemia as a model.
Sera from bisalbuminemic chicken-turkey hybrids contain two albumins in equal amounts. These are observed as inherited electrophoretic variants and originate from the respective chicken and turkey parents. Sera from the hybrid birds served as a model system by which fractionating and indentification procedures for evaluating serum albumin variants were compared. The two albumins in the hybrid were isolated with preparative polyacrylamide gel electrophoresis (PAGE) and starch block preparative electrophoresis. Isoelectric focusing of the hybrid albumins resulted in the isolation of the turkey albumin. Interference of ampholines prevented the complete isolation of the chicken albumin. The two albumins in the hybrid have identical molecular weights and cannot be identified by sedimentation coefficient, gel filtration behavior, or sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Because of the close relatedness the chicken and turkey albumins in the hybrid cross reacted with rabbit anti-hybrid serum as well as with rabbit anti-chicken anti-turkey sera.
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D002645:Chickens; D004588:Electrophoresis, Cellulose Acetate; D004592:Electrophoresis, Starch Gel; D006824:Hybridization, Genetic; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D008954:Models, Biological; D012709:Serum Albumin / Q000302:isolation & purification*; D014422:Turkeys
D001798:Blood Proteins; D012709:Serum Albumin