The amino-acid sequence of the alpha-crystallin A chains of red kangaroo and Virginia opossum.
The amino acid sequence of the A chain of the eye lens protein alpha-crystallin from the red kangaroo (Macropus rufus) was completely determined by manual Edman degradation of tryptic, thermolytic and cyanogen bromide peptides. The sequence of the alpha-crystallin A chain from the Virginia opossum (Didelphis marsupialis) was deduced from amino acid analyses and partial Edman degradation of peptides. The 173-residue A chains of kangaroo and opossum differ in six positions, whereas comparison with the bovine alpha-crystallin A chain reveals 17 and 22 substitutions, respectively. Most substitutions occur in the COOH-terminal part of the chain.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D003459:Crystallins; D003488:Cyanogen Bromide; D007614:Macropodidae / Q000378:metabolism*; D008394:Marsupialia / Q000378:metabolism*; D009893:Opossums / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D013045:Species Specificity
D000596:Amino Acids; D003459:Crystallins; D010446:Peptide Fragments; D003488:Cyanogen Bromide