[Analysis of polysaccharides used as food additives iv. Gaschromatographic identification and determination of neutral components in polysaccharide-hydrolysates (author's transl)].
The monosaccharides, which are obtained by hydrolytic cleavage of the polysaccharid containing stabilisators, can be separated gaschromatographically by the way of their aldonitrilacetates. After hydrolysis, the mixtures are neutralized by an anion exchanger, evaporated to dryness, converted in pyridin with hydroxylamin to the aldoximes and hereafter with acetic anhydride to the aldonitrilacetates. Dissolved in chloroform, these compounds are used for gaschromatographic separations; only one peak is obtained for each sugar. This is a great advantage to the previous methods, where the anomeric constellations lead up of five peaks for one sugar compound and for complicated sugar mixture, it therefore was difficult to evaluate their gaschromatograms.
D002849:Chromatography, Gas / Q000379:methods; D005503:Food Additives / Q000032:analysis*; D006868:Hydrolysis; D011134:Polysaccharides / Q000032:analysis*
D005503:Food Additives; D011134:Polysaccharides
Beiträge zur Analytik von als Lebensmittelzusatzstoffe verwendeten Polysacchariden. IV. Gaschromatographische Identifizierung und Bestimmung neutraler Bausteine von Polysaccharid-Hydrolysaten