Factors affecting prostaglandin synthesis by rat skin microsomes.
Prostaglandin synthesis by rat skin was measured by a radiochemical method and the adrenochrome spectrophotometric method. About 70% of the total enzyme activity was located in the microsomal fraction. Incubation with (1-14C) arachidonic acid resulted in the formation of prostaglandins F2alpha, E2 and D2. The biosynthesis of these 3 prostaglandins was stimulated by reduced glutathione and hydroquinone, and was markedly dependent on pH. A number of non-steroid anti-inflammatory drugs produced a dose related inhibition of prostaglandin biosynthesis. Five potent corticosteroid anti-inflammatory agents previously found to be inhibitory when a skin homogenate was used did not inhibit prostaglandin synthesis by the microsomal fraction.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001095:Arachidonic Acids / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology; D006873:Hydroquinones / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D011506:Proteins / Q000494:pharmacology; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
D000893:Anti-Inflammatory Agents; D001095:Arachidonic Acids; D006873:Hydroquinones; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D011506:Proteins; D005978:Glutathione; D007213:Indomethacin