Fragmentation of rat serum albumin by cyanogen bromide cleavage and the amino acid sequences of four fragments.
Rat serum albumin was cleaved into seven peptides by cyanogen bromide treatment followed by reduction and carboxymethylation. The amino acid composition and the N-terminus of each peptide and the amino acid sequences of four peptides were determined by conventional methods. An alignment of these peptides in the original protein was proposed in the light of the established sequences of bovine and human plasma albumin.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D051381:Rats; D012709:Serum Albumin; D013045:Species Specificity; D014357:Trypsin
D000596:Amino Acids; D010446:Peptide Fragments; D012709:Serum Albumin; D002918:Chymotrypsin; D014357:Trypsin; D003488:Cyanogen Bromide