Further studies on section thickness measurement.
A rapid, simple, accurate and reproducible technique for measuring section thickness using an instrument known as a surfometer is described. It has shown that microtome settings cannot be relied upon as a means of monitoring the thickness of sections of quenched or wax-embedded tissue. However, there appeared to be a linear relationship between microtome setting and the thickness of dewaxed sections. Microtome settings seem to provide an accurate indication of the thickness of araldite sections.
D000818:Animals; D006651:Histocytochemistry / Q000295:instrumentation*; D008099:Liver / Q000166:cytology; D008722:Methods; D051379:Mice