An electron microscopic and cytochemical detection of concanavalin A receptors on the cell membrane of Leishmania braziliensis guyanensis.
Promastigotes of Leishmania braziliensis quyanensis cultivated in the NNN medium agglutinate with concanavalin A (con A). The protozoon was agglutinated at different concentrations of con A. Maximal agglutination was obtained with 150 mug/ml. Three types of agglutination were observed: flagellar-flagellar, flagellar-body and body-body. Cell surface polysaccharides, glycoproteins or glycolipids were demonstrated using the periodic acid-thiosemicarbazide-silver proteinate technic. A con A-horseradish peroxidase-diaminobenzidine (DAB) technic was used to detect con A receptors on the cell membrane of the parasite.
D000818:Animals; D001666:Binding Sites, Antibody; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D003208:Concanavalin A / Q000378:metabolism*; D006017:Glycolipids / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006651:Histocytochemistry; D007891:Leishmania / Q000032:analysis* / Q000648:ultrastructure; D011134:Polysaccharides / Q000032:analysis
D002241:Carbohydrates; D006017:Glycolipids; D006023:Glycoproteins; D011134:Polysaccharides; D003208:Concanavalin A