Evidence that vitamin A is not required for the biosynthesis of ovalbumin in chicks.
Four-day-old pullets fed a vitamin A-deficient diet were stimulated daily with 1 mg 17beta-estradiol-3-benzoate/day for 6 to 19 days. The onset of vitamin A deficiency had no effect on oviduct growth in these chicks; even though vitamin A-deficient chicks showed a severe decline in growth rate while controls (fed the same diet supplemented with retinyl palmitate) continued to grow, estrogen stimulated resulted in similar oviduct size. Ovalbumin concentrations of estrogen-stimulated chicks were determined by immunoprecipitation of the soluble protein supernatant fraction of oviduct. The concentration of ovalbumin in oviducts of chicks fed a vitamin A-supplemented diet was similar in the concentration in oviducts of chicks fed a vitamin A-deficient diet. The incorporation of [3H]glucosamine and 14C-amino acids into immunoprecipitable ovalbumin, following the in vitro incubation of minced oviduct, indicated that ovalbumin synthesis was not affected by vitamin A deficiency. The specific activity of incorporated [3H]glucosamine, the 14C-amino acid incorporation into ovalbumin, the relative rate of ovalbumin synthesis, and the relative effiency of [3H]glucosamine incorporation into ovalbumin were each similar between the two diet groups. The relative efficiency of [3H]glucosamine incorporation into sodium dodecyl sulfate and dithiothreitol extractable membranous proteins of oviduct was not affect by vitamin A deficiency.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D002645:Chickens / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D005944:Glucosamine / Q000378:metabolism; D009929:Organ Size; D010047:Ovalbumin / Q000096:biosynthesis*; D010057:Oviducts / Q000187:drug effects / Q000502:physiology*; D011233:Precipitin Tests; D011506:Proteins / Q000378:metabolism; D014802:Vitamin A Deficiency / Q000378:metabolism*
D000596:Amino Acids; D011506:Proteins; D004958:Estradiol; D010047:Ovalbumin; D005944:Glucosamine