The in vitro effect of diethylaminoethyl dextran on stimulation of mouse lymphocytes, and on the mitogenic activity of concanavalin A and lipopolysaccharide.
Unpurified lymphocytes from spleen, lymph nodes, thymus, and T and B cell-enriched fractions of spleen cells from NMRI mice as well as lymphocytes of nude mice were cultured and stimulation of the lymphocytes by DEAE-D, Con A and LPS and combinations thereof was measured by incorporation of 3H-thymidine. It was demonstrated that DEAE-D is a rather weak mitogen for mouse lymphocytes, acting apparently on B cells as well as on T cells. Frequency and intensity of lymphocyte stimulation by the T cell mitogen Con A and the B cell mitogen LPS was much better. When combinations of DEAE-D with Con A or LPS, respectively, were used significant enhancement of lymphocyte stimulation occurred, as compared to the mitogens and DEAE-D alone. This enhancement was more prominent with the DEAE-D/Con A combination than with the DEAE-D/LPS combination, and more pronounced with the T cell-rich lymphocyte preparations than with the B cell-rich suspensions. The results are discussed and it is concluded that DEAE-D enhances both T cell and B cell functions, however, T cell functions are favoured. This enhancement is assumed to be mediated by a membrane effect of DEAE-D, and to be responsible for th immunological adjuvant effect of DEAE-D.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D003637:DEAE-Dextran / Q000494:pharmacology*; D003911:Dextrans / Q000031:analogs & derivatives*; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008934:Mitogens
D003911:Dextrans; D008070:Lipopolysaccharides; D008934:Mitogens; D003208:Concanavalin A; D003637:DEAE-Dextran