An evaluation of an assay for DNA synthesis in lymphocytes with [3H]thymidine and harvesting on to glass fibre filter discs.
Different grades of glass fibre filter paper have been compared for harvesting cells labelled with tritium using a semi-automatic harvester. Grade GF/C (Whatman Ltd) was selected for routine use although GF/B was found to be useful for whole blood cultures. The factors affecting the choice of thymidine concentration and activity are discussed and 1 mjCi or [3H]thymidine (5 Ci/mMol)/0.2 ml culture is shown to give a linear relationship with total DNA synthesis for up to 22 h.
D000818:Animals; D002452:Cell Count / Q000379:methods; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D005374:Filtration / Q000295:instrumentation; D005898:Glass; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D013154:Spleen / Q000166:cytology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D004247:DNA; D013936:Thymidine