The fine structure of the embryo during the gastrula stage of Comanthus japonica (Echinodermata: Crinoidea).
The fine structure of the embryo of Comanthus has been described by scanning and transmission electron microscopy at two-hourly intervals throughout the gastrula stage (from the fifth through the fifteenth hours of development). At 5 hr, gastrulation has occurred in the absence of any structure comparable to the echinoid hyaline layer; therefore, at least one important mechanism proposed for echinoid gastrulation cannot occur in this crinoid. At 7 hr, the blastocoelic basal lamina has formed, and all ectodermal and entodermal nuclei contain dense aggregates, which are probably perichromatin fibrils. At 9 hr, the blastocoel contains mesenchyme cells, presumably of entodermal origin. At 11 hr, ciliogenesis has started at the apical surfaces of the ectoderm cells and at the archenteral surfaces of the entoderm cells; many of the newly formed cilia are swollen subterminally. At 13 hr, a conspicuous glycocalyx is beginning to cover the apical ends of the ectoderm cells, and the fertilization membrane is beginning to dissolve from its inner surface. Between 5 and 13 hr, there is a gradual development of a junctional complex associating the apicolateral margins of the ectoderm cells; the zonula adherens part of the complex appears at 5 hr and is well developed by 7 hr, and then the septate junction part of the complex appears at 9 hr and is well developed by 13 hr. At 15 hr, the blastopore has closed, the ectodermal glycocalyx is fully developed, some mesenchyme cells appear to be differentiating into skeleton forming cells, and the fertilization membrane is in the last stages of dissolution.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004447:Echinodermata / Q000196:embryology*; D004475:Ectoderm / Q000648:ultrastructure; D004625:Embryo, Nonmammalian / Q000648:ultrastructure*; D004707:Endoderm / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D007365:Intercellular Junctions / Q000648:ultrastructure; D009940:Organoids / Q000648:ultrastructure; D013997:Time Factors