Comparative activity of arylsulphatases A and B on two synthetic substrates.
Rat liver and human skin fibroblasts arylsulphatase A and B activities on both 4-methylumbelliferyl sulphate and 4-nitrocatechol sulphate were compared. The intracellular distribution of activity differed markedly when 4-methylumbelliferyl sulphate was used from that observed with 4-nitrocatechol sulphate. No discrimination between control and metachromatic leucodystrophy or mucopolysaccharidosis (type VI) could be achieved when 4-methylumbelliferyl sulphate was used as substrate. These results contrast sharply with those obtained with 4-nitrocatechol sulphate and cast doubt on the validity of 4-methylumbelliferyl sulphate as substrate for the determination of arylsulphatase A and B activities.
D000818:Animals; D002396:Catechols; D002803:Chondro-4-Sulfatase / Q000378:metabolism*; D006801:Humans; D006923:Hymecromone; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D051381:Rats; D012867:Skin / Q000201:enzymology; D013429:Sulfatases / Q000378:metabolism*
D002396:Catechols; D006923:Hymecromone; D013429:Sulfatases; D002803:Chondro-4-Sulfatase; C001833:4-nitrocatechol