[Use of Soviet semipermeable acetate-cellulose membranes for concentration and purification of typhoid antigens].
A study was made of a possibility of using Soviet made membranes "Vladipor" with a pore diameter of 16--17 nm for the purpose of concentration and purification of antigenic extracts from stab cultures of S. typhi obtained by the action of typsin, hydrogen peroxide and detergents. Preparations, complete by antigenic structure, were obtained by ultrafiltration, with a good yield. The maximal rate of ultrafiltration was noted in case of "peroxide" antigenic preparations. The use of diafiltration provided a significant reduction of the ash content in the end products.
D000085:Acetates; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002482:Cellulose; D003902:Detergents; D006861:Hydrogen Peroxide; D008851:Micropore Filters; D012485:Salmonella typhi / Q000276:immunology*; D014357:Trypsin; D014462:Ultrafiltration
D000085:Acetates; D000942:Antigens, Bacterial; D003902:Detergents; D002482:Cellulose; D006861:Hydrogen Peroxide; D014357:Trypsin
Ispol'zovanie otechestvennykh polupronitsaemykh atsetattselliuloznykh membran dlia kontsentratsii i ochistki briushnotifoznykh antigenov