Dielectric increment of low molecular weight RNA in dioxane after application of static electric fields.
A soluble RNA with a molecular weight between 15 000 and 23 000 was extracted from calf thymus and chromatographically purified. The RNA was slightly soluble in doxane (approx. 2.5 mug/ml) and RNA-dioxane solutions were used to fill a cell (452.02 pF capacitance) to which static electric fields of variable strength were applied. The dielectric permittivity of the solutions was measured at a fixed time interval after the application of the electric field. The RNA solutions showed a dielectric increment proportional to the strength of the applied field and to the RNA concentration. The existence of a hystersis effect was proven and the effect of the electric field on the RNA molecule might be related to a dielectric saturation phenomenon parallel to long-term changes of the molecule.
D000818:Animals; D002417:Cattle; D004058:Diffusion; D004146:Dioxanes; D004147:Dioxins; D004553:Electric Conductivity; D004558:Electric Stimulation; D008433:Mathematics; D008970:Molecular Weight; D012313:RNA / Q000302:isolation & purification; D013816:Thermodynamics; D013950:Thymus Gland
D004146:Dioxanes; D004147:Dioxins; D012313:RNA