A laminar flow superfusion technique with much increased sensitivity for the detection of smooth muscle-stimulating substances.
The classical superfusion technique was modified by immersing the isolated assay tissues in mineral oil, making it possible to superfuse the preparations through the oil with a drip of Krebs solution at very low flow rates (0.1 - 0.2 ml/min). In this way the sensitivity of the preparation for the detection and quantitation of endogenous biologically active substances was extended to the picogram level.
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001920:Bradykinin / Q000494:pharmacology*; D002415:Cats; D009130:Muscle, Smooth / Q000187:drug effects*; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D013268:Stimulation, Chemical
D011458:Prostaglandins E; D000804:Angiotensin II; D001920:Bradykinin