title: Isolement et caractérisation de deux subunites constitutives des glycoproteines de structure du tissu sous cutané de lapin.
abstract: Structural glycoproteins have been extracted by 8 M ureau from the insoluble residue remaining after collagenase digestion of rabbit dermis and purified by Sepharose 4 B chromatography. After reduction and alkylation, Dowex 1 x 2 chromatography allowed separation of two structural glycoproteins (D1 and D2) in an homogenous state as shown by chromatographic and electrophoretic behaviour as well as N terminal amino acid determination. These two glycoproteins have a molecular weight of about 16 000. Their amino acid compositions (very similar), are characterized by a high level of dicarboxylic amino acids and the absence of hydroxyproline and hydroxylysine. The less acidic glycoprotein (D1) has glycine for N terminal amino acid and contains 10.4 percent of bound carbohydrates. The glycoprotein D2 contains 5.1 percent of bound carbohydrates and its N terminal amino acid is glutamic acid.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000600:Amino Acids, Dicarboxylic / Q000032:analysis; D000818:Animals; D006023:Glycoproteins / Q000302:isolation & purification; D006901:Hydroxylysine / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D008970:Molecular Weight; D011817:Rabbits; D012867:Skin / Q000032:analysis*
chemical_list: D000596:Amino Acids; D000600:Amino Acids, Dicarboxylic; D006023:Glycoproteins; D006901:Hydroxylysine; D006909:Hydroxyproline