A method for the collection of lymph from the prescapular lymph node of unanaethetized sheep.
A method for cannulating the efferent duct of the prescapular lymph node of sheep was developed and carried out on over 100 occasions so that lymph could be collected from unanaesthetized sheep under physiological conditions. On average the lymph flowed at 9 ml/h and contained 15 X 10(6) lymphocytes/ml. The surgical procedure and relevant anatomical details are described.
D000818:Animals; D008196:Lymph; D008198:Lymph Nodes / Q000033:anatomy & histology; D012756:Sheep / Q000033:anatomy & histology / Q000502:physiology*; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*