title: Effect of chronic glucagon-administration on the digestive and absorptive function of rat small intestine in vivo.
abstract: Endogeneous hyperglucagonemia is observed in experimental diabetes mellitus and semistarvation, conditions associated with an increased intestinal absorptive function. To examine whether glucagon might exert a similar adaptive response on intestinal digestive-absorptive function like experimental diabetes mellitus the effect of chronic glucagon administration on intestinal transport of 3-0-methyl-D-glucose, water, sodium, potassium, and D-glucose induced transmural potential difference (PD) was examined by an in vivo perfusion technique in rat small intestine. Chronic administration of glucagon (100 mug twice daily) for 5 days resulted in increased absorption of 3-0-methyl-D-glucose, water, sodium and potassium as well as in an increase of D-glucose induced PD. A similar, but more pronounced augmentation of D-glucose induced PD was observed in the jejunum of streptozotocin-diabetic rats. Disaccharidase (maltase, sucrase, trehalase, lactase) and alkaline phosphatase activities were not affected in intestinal mucosa of glucagon-treated rats compared to controls. It cannot be decided from these results whether hyperglucagonemia is responsible for the adaptive intestinal changes observed in experimental diabetes mellitus.
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D003920:Diabetes Mellitus / Q000378:metabolism; D004186:Disaccharidases / Q000032:analysis; D005260:Female; D005934:Glucagon / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects*; D007583:Jejunum / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D008757:Methylglucosides / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D008757:Methylglucosides; D014867:Water; D005934:Glucagon; D012964:Sodium; D000469:Alkaline Phosphatase; D004186:Disaccharidases; D011188:Potassium