Thermal inactivation of maltase and its application to temperature-sensitive mutants of yeast.
Three maltases have been detected in each of a variety of strains of Saccharomyces. These are present in constant relative amounts in a particular strain and are characterized by reproducible first order thermal decay constants. Four of six mutants, temperature-sensitive for maltose fermentation, lack the maltase of intermediate stability. This deficiency appears to be the basis of the temperature sensitivity, and it is concluded that these strains carry a mutation in the structural gene for that maltase.
D005796:Genes; D005959:Glucosidases / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D009154:Mutation; D012440:Saccharomyces / Q000201:enzymology*
D005959:Glucosidases