Automated method for L-carnitine determination.
Because of renewed interest in a possible connection between carnitine, lipid disorders, and myopathy, an automated method of analysis is desirable. Deproteinization of serum by use of membrane filter cones and automated assay with a bichromatic analyzer (the ABA-100) substantially increases efficiency without sacrificing the specificity and accuracy of the original manual enzymatic method. The described procedure allows for analysis of 80 speciments a day and is thus suitable for screening of selected populations. Normal values found in blood sera of adults were in the range of 25.0-73.8 mu mol/liter and the method has sufficient sensitivity to accurately measure concentrations as small as 10 mu mol/liter.
D000328:Adult; D001322:Autoanalysis; D002331:Carnitine / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D012016:Reference Values; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
D002331:Carnitine