[A quantitative method of determining lecithinases and lipases in microorganisms].
The paper presents a method for quantitative assay of lecithinases and lipases in microorganisms. The method is based on the assessment of changes in the composition of lipid components of yolk media under the influence of corresponding enzymic systems of bacteria. The technique involved lipid extraction from yolk media after cultivation of the microorganisms tested, chromatographic separation by thin-layer chromatography on silicagel into individual fractions, development in iodine vapours and quantitative measurement of lipid components.
D001419:Bacteria / Q000201:enzymology*; D008049:Lipase / Q000032:analysis*; D008722:Methods; D010740:Phospholipases / Q000032:analysis*; D011511:Proteus / Q000201:enzymology; D013211:Staphylococcus aureus / Q000201:enzymology
D010740:Phospholipases; D008049:Lipase
Kolichestvennyĭ metod opredeleniia letsitinaz i lipaz u mikroorganizmov