Endotoxin-stimulated spleen cells: dissociation between DNA synthesis and IgM production at the cellular level.
LPS stimulation of mouse spleen cells in the presence of Hu resulted in almost total suppression of [3H]thymidine incorporation without affecting the percentage of cells induced to produce IgM. Utilizing a method which permitted the simultaneous measurement of IgM production and [3H]thymidine incorporation in individual cells, it was demonstrated directly that LPS stimulation of IgM production can occur in the absence of DNA synthesis.
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004731:Endotoxins / Q000494:pharmacology*; D006918:Hydroxyurea / Q000494:pharmacology; D007075:Immunoglobulin M / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000378:metabolism*
D004731:Endotoxins; D007075:Immunoglobulin M; D004247:DNA; D006918:Hydroxyurea