Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma.
The dye Cibacron Blue F-3-GA was conjugated to Sepharose to provide an affinity column for serum albumin. Passage of whole human plasma through a column of Cibacron Blue-Sepharose results in the removal of approx. 98% of the albumin. The latter can be quantitatively recovered by desorption with NaSCN. Albumin-depleted plasma can be readily resolved into discrete fractions by a combination of conventional biochemical techniques. In particular, the resolution of plasma proteins with properties similar to those of native human plasma albumin can readily be accomplished by ion-exchange chromatography of the Sepharose-dye-treated plasma on DEAE-cellulose.
D000645:Ammonium Sulfate; D000873:Anthracenes; D001798:Blood Proteins / Q000302:isolation & purification*; D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D004396:Coloring Agents; D000431:Ethanol; D006801:Humans; D012685:Sepharose; D012709:Serum Albumin / Q000302:isolation & purification*
D000873:Anthracenes; D001798:Blood Proteins; D004396:Coloring Agents; D012709:Serum Albumin; D000431:Ethanol; D012685:Sepharose; D000645:Ammonium Sulfate