The 7-methylbenz(a)anthracene deoxyribonucleoside products isolated from DNA after metabolism of the carcinogen by rat liver microsomes in the presence of DNA.
Metabolism of 7-methylbenz[a]anthracene (7MeBA) by 3-methylcholanthrene-induced rat liver microsomes in the presence of added native or denatured DNA resulted in covalent binding of the hydrocarbon to the nucleic acid. Enzymatic degradation and column chromatographic fractionation showed that the hydrocarbon-deoxyribonucleoside products were separable from the products similarly obtained from DNA having 7MeBA bound following treatment of mouse embryo cells in culture with this hydrocarbon. Comparison of the microsome catalysed hydrocarbon-deoxyribonucleoside products with those obtained by reaction with DNA of 7MeBA-5,6-oxide suggested that this K-region epoxide made a significant contribution to the liver microsome-induced DNA binding.
D000818:Animals; D001551:Benz(a)Anthracenes / Q000378:metabolism*; D001665:Binding Sites; D002460:Cell Line; D002850:Chromatography, Gel; D004247:DNA / Q000378:metabolism*; D003853:Deoxyribonucleosides / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats
D001551:Benz(a)Anthracenes; D003853:Deoxyribonucleosides; D004247:DNA