Acceleration of the reaction between thrombin and antithrombin III by non-stoichiometric amounts of heparin.
The accelerating effect of heparin on the reaction between purified human antithrombin and thrombin has been investigated by measuring the amount of thrombin inactivated during a short incubation of the enzyme with the inhibitor in the presence and absence of non-stoichiometric amounts of heparin. It was demonstrated that one molecule of heparin was able to promote the binding of a large number of antithrombin molecules to thrombin. Thus heparin may affect the rate of the inactivation of thrombin by antithrombin in a catalytic manner.
D000991:Antithrombins / Q000302:isolation & purification / Q000378:metabolism; D002846:Chromatography, Affinity; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D011485:Protein Binding; D013917:Thrombin / Q000378:metabolism
D000991:Antithrombins; D046911:Macromolecular Substances; D006493:Heparin; D013917:Thrombin