The free-falling drop detector--a nover fluorescence detector for high-performance liquid chromatography.
A novel fluorescence detector for high-performance liquid chromatography has been designed to minimize the instrumental limitations of conventional flow-through, fluorometers. This unit contributes less than 5 mul to the "dead volume" of the chromatographic system. Sensitivity comparable to that of existing flow-through fluorometers has been attained without optimizing instrumental parameters. Performance of the unit has been evaluated with reference samples containing saccharides typical of glycosphingolipids. Future improvements are discussed.
D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D002852:Chromatography, Ion Exchange; D005069:Evaluation Studies as Topic; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
D002241:Carbohydrates; D009005:Monosaccharides; D009844:Oligosaccharides