Considerations into the mechanism of estrogen-stimulated uterine prostaglandin synthesis.
Progesterone priming of the ovariectomized rat, followed by a single injection of estradiol-17beta (10 mug) is followed by an increased uterine synthesis of both PGF and PGE. The administration of an estrogen antagonist (MER-25; 10 mg) concomitantly with estradiol had no effect on uterine prostaglandin synthesis. Similarly, the administration of either Actinomycin D or cycloheximide, antibiotics demonstrated to inhibit mRNA and protein synthesis, respectively, is without effect on estrogen-stimulated uterine prostaglandin synthesis. These results are considered with regard to the classic receptor theory of estrogen action and are a preliminary indication that estrogen-stimulated uterine prostaglandin synthesis may not require those receptor mediated events.
D000818:Animals; D002369:Castration; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D004978:Ethamoxytriphetol / Q000494:pharmacology; D005260:Female; D009929:Organ Size; D011374:Progesterone / Q000494:pharmacology; D011458:Prostaglandins E / Q000096:biosynthesis*; D011460:Prostaglandins F / Q000096:biosynthesis*; D051381:Rats; D013268:Stimulation, Chemical; D014599:Uterus / Q000033:anatomy & histology / Q000378:metabolism*
D011458:Prostaglandins E; D011460:Prostaglandins F; D003609:Dactinomycin; D011374:Progesterone; D004958:Estradiol; D004978:Ethamoxytriphetol; D003513:Cycloheximide