Production of tumor-specific inducer of cellular cytotoxicity by lethally irradiated mice.
Antisera taken 1 or 2 days after inoculation of BALB/c mice with transplantable sarcoma cells or Moloney sarcoma virus (MSV) induce tumor-specific cell-dependent cytotoxicity in vitro. In the present experiments, lethally irradiated ("immunosuppressed") mice were tested for the early appearance of the serum factor responsible for this anti-serum-dependent cell-mediated cytotoxicity (E-ADC). BALB/c mice were infected with MSV, syngeneic sarcoma cells or sheep red blood cells 24 h following irradiation with 900 R. Sera were obtained from MSV and tumor cell recipients 48 or 72 h later and tested for E-ADC activity. Spleen cells from SRBC recipients were tested at day 4 or 5 for ability to form direct plaques in a modified Jerne plague assay. Although the anti-SRBC response was obliterated in the irradiated mice, the E-ADC response appeared to be unimpaired. These studies indicate that newly synthesized immunoglobulin is not required for the formation of the E-ADC factor.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007106:Immune Sera; D007111:Immunity, Cellular; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008979:Moloney murine leukemia virus; D009368:Neoplasm Transplantation; D012513:Sarcoma, Experimental / Q000276:immunology*; D014183:Transplantation, Heterologous; D014185:Transplantation, Isogeneic; D010948:Viral Plaque Assay
D007106:Immune Sera