Measurement of androstanediol in plasma in a radioimmunoassay using celite column chromatography.
A rapid and relatively simple, but specific, radioimmunoassay for the potent androgen, androstanediol (3 alpha-diol), is described. Despite the availability of a nonspecific C-19 androgen antibody requiring a 17 beta-hydroxy group, androstanediol can be measured in plasma by prior purification of a plasma solvent extract using a Celite microcolumn. Values obtained do not differ from those previously reported using more complicated chromatographic techniques.
D015113:Androstane-3,17-diol / Q000097:blood* / Q000276:immunology; D000731:Androstanes / Q000097:blood*; D002845:Chromatography / Q000295:instrumentation*; D005260:Female; D006801:Humans; D008297:Male; D011863:Radioimmunoassay / Q000379:methods*
D000731:Androstanes; D015113:Androstane-3,17-diol