Transfer RNA species in tumors of different growth rates.
Tyrosyl-, histidyl-, lysyl-, and phenylalanyl-tRNA's from 3 tumors (DBAH, DBAH, and DBA), differing in growth rates and from host mammary glands and liver, were compared by means of methylated albumin kieselguhr (MAK) column and by reverse-phase-5 chromatography. The elution profiles of lysyl-tRNA's from DBAH and DBA, phenylalanyl Trna's from DBAH and DBAH, and histidyl-tRNA's from DBA3 tumors exhibited extra isoaccepting species, compared with host liver and mammary glands. The distribution of acylatable tyrosyl-tRNA's in DBA3 and DBAH, phenylalanyl-tRNA's in DBAH and DBAH2, and histid-l-tRNA's in DBA3 is higher than that in liver, whereas no appreciable differences were observed in the lysyl-tRNA contents of the tumors and liver. The chromatographic alternations appeared to be a property of the tumor tRNA's and not due to differences in aminoacyl-tRNA synthetases or due to the aggregation of tRNA's. The structural and functional significance of these findings are discussed.
D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D005260:Female; D006639:Histidine / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008223:Lymphoma / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D013997:Time Factors; D014443:Tyrosine / Q000378:metabolism
D012334:RNA, Neoplasm; D014443:Tyrosine; D010649:Phenylalanine; D006639:Histidine; D012343:RNA, Transfer; D008239:Lysine