Isolation and characterization of 6-hydroxymethylpterin as the Crithidia growth-promoting factor from spinach chloroplasts.
2-Amino-4-hydroxypteridine compounds were isolated as the Crithidia factor from spinach chloroplasts by DEAE-Sephadex A-50, DEAE-cellulose, Sephadex G-25, and thin-layer chromatography. One of the compounds was characterized as 6-hydroxymethylpterin by gas-liquid chromatography-mass spectrometry and by comparison with authentic specimen.
D002736:Chloroplasts / Q000032:analysis*; D002849:Chromatography, Gas; D056890:Eukaryota / Q000254:growth & development; D013058:Mass Spectrometry; D010944:Plants; D011622:Pterins / Q000032:analysis / Q000302:isolation & purification*
D011622:Pterins; C013044:6-hydroxymethylpterin