[Insulin and glucagon secretion in the isolated perfused rat pancreas].
For the study of insulin and glucagon secretion at the isolated perfused rat pancreas are used, as a rule, synthetic perfusion media that have to meet two requirements: They have to ensure the biological function of the perfused organ for 2 hrs, and must not interfere with radioimmunological insulin and glucagon determination. An appropriate medium is the Krebs-Ringer bicarbonate buffer at pH 7.4 with addition of 4% dextran (MG 75000) and 0.5% human serum albumin; it allows one to produce, after permanent glucose stimulation, a biphasic insulin secretion. Infusion media based on gelatine or polyvinylpyrrolidone proved inadequate for these studies.
D000818:Animals; D003470:Culture Media; D003911:Dextrans; D005934:Glucagon / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008722:Methods; D010101:Oxygen Consumption; D010179:Pancreas / Q000378:metabolism*; D010477:Perfusion; D011863:Radioimmunoassay; D051381:Rats; D012709:Serum Albumin
D003470:Culture Media; D003911:Dextrans; D007328:Insulin; D012709:Serum Albumin; D005934:Glucagon
Untersuchung der Insulin- und Glukagonsekretion am isoliert perfundierten Rattenpankreas