Persistence of the ten-nucleotide repeat in chromatin unfolded in urea, as revealed by digestion with deoxyribonuclease i.
It is shown by enzymatic digestion of chromatin from rat liver or Guerin ascites tumour (GAT) that treatments, which abolish the 180 base pair repeat, as revealed by digestion with micrococcal nuclease (shearing in salt solutions of medium ionic strength, sonication, fixation with formaldehyde in the presence of 5 M urea), have little effect on the 10 nucleotide repeat, observed in deoxyribonuclease I digests.
D001483:Base Sequence; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA; D004273:DNA, Neoplasm; D003851:Deoxyribonucleases; D008099:Liver; D008836:Micrococcal Nuclease; D009374:Neoplasms, Experimental; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D014508:Urea
D002843:Chromatin; D004273:DNA, Neoplasm; D009838:Oligodeoxyribonucleotides; D014508:Urea; D004247:DNA; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease