Studies of intracellular distribution of acid phosphatase 5 in the spleen cells of leukemic reticuloendotheliosis by isopycnic gradient centrifugation.
A fresh spleen sample obtained from a patient with leukemic reticuloendotheliosis was homogenized and subjected to centrifugation on a sucrose density gradient. A major portion of acid phosphatase band 5 was observed in the lysosome, confirming that the elevated phosphatase activity in the neoplastic spleen is a lysosomal enzyme. However, a significant amount of brand 5 was also observed in the microsome. The microsomal and lysosomal enzymes have different affinity to CM-cellulose. The relationship between lysosomal and microsomal enzymes has not been established.
D000135:Acid Phosphatase / Q000378:metabolism*; D006801:Humans; D007938:Leukemia / Q000201:enzymology*; D007958:Leukocyte Count; D008206:Lymphatic Diseases / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D013154:Spleen / Q000201:enzymology*; D013156:Splenectomy
D000135:Acid Phosphatase