The mass spectrometric identification of dipeptides in the urine of a patient suffering from chronic skin ulceration and oedema.
The identification of a complex ninhydrin positive mixture present in the urine of a child suffering from chronic skin ulceration and oedema by direct chemical ionisation mass spectrometric analysis is described. The compounds were shown to be dipeptides, of which glycylproline was the major constituent. At least 15 dipeptides were identified in the urine, most of which contained proline or hydroxyproline in the carboxy terminal position. The results suggested that the patient suffered from a defect in collagen metabolism. This hypothesis was subsequently confirmed by a grossly diminished level of prolidase in cultured fibroblasts and erythrocytes.
D000596:Amino Acids / Q000652:urine; D002908:Chronic Disease; D004151:Dipeptides / Q000652:urine*; D004487:Edema / Q000150:complications / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013058:Mass Spectrometry; D012883:Skin Ulcer / Q000150:complications / Q000652:urine*
D000596:Amino Acids; D004151:Dipeptides