High-pressure liquid chromatographic analysis of tolbutamide in serum.
A high-pressure liquid chromatographic (HPLC) analysis of tolbutamide in serum is described. The assay requires only 1 ml of serum and is capable of measuring as little as 2 mug of tolbutamide. The metabolites of tolbutamide do not interfere in the assay. Human serum samples, taken after a 1-g oral dose of tolbutamide, were analyzed by the HPLC and an existing GLC procedure, and the results are compared.
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008722:Methods; D013997:Time Factors; D014044:Tolbutamide / Q000097:blood*
D014044:Tolbutamide