Mapping of several abnormal hemoglobins by horizontal polyacrylamide gel isoelectric focusing.
The relative mobilities of serveral human hemoglobin variants were studied by use of an isoelectric focusing method in a thin-layer horizontal polyacrylamide gel. The technic is described in detail and a preliminary mapping of these variants is presented. It appears that thin-layer gel isoelectric focusing is a method that is rapid, highly reproducible and easily applicable in laboratories concerned with the study of hemoglobinopathies.
D000595:Amino Acid Sequence; D001797:Blood Protein Electrophoresis; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D008403:Mass Screening; D008722:Methods; D009154:Mutation; D013997:Time Factors
D006455:Hemoglobins, Abnormal