title: Technetium-99m labeling of human monocytes for chemotactic studies.
abstract: Although the current use of 51Cr for the evaluation of monocyte chemotaxis has yielded encouraging results, certain properties of this radionuclide leave room for improvement. Technetium-99m was evaluated as an alternative label for this purpose. A cell loss of 50% was found, but the recovered cells showed excellent viability and function. Chemotaxis was measured using a modified Boyden's chamber and a lymphocyte-derived chemotactic factor. The chemotactic properties of 99mTc-labeled human monocytes were preserved, and an excellent correlation between radioactive measurement and microscopic counting of migrating cells was observed.
mesh_terms: D002634:Chemotaxis, Leukocyte; D006801:Humans; D066298:In Vitro Techniques; D007553:Isotope Labeling / Q000379:methods*; D009000:Monocytes / Q000502:physiology; D013667:Technetium
chemical_list: D013667:Technetium