The effects of various agents in vitro on homocarnosine-carnosine synthetase from rat brain.
The present paper reports the first evidence that homocarnosine-carnosine synthetase from rat brain requires free sulfhydryl groups for activity. The activity of the synthetase can be stabilized by dithioerythritol and inhibited strongly by Cu2+, Cd2+, Hg2+, Zn2+, and to a lesser extent by Ca2+, Ni2+, Li, chlorpromazine, alpha-methyl DOPA and norepinephrine at the concentrations tested.
D000409:Alanine / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002336:Carnosine / Q000031:analogs & derivatives / Q000378:metabolism*; D002413:Cations, Divalent / Q000494:pharmacology; D002729:Chloromercuribenzoates / Q000494:pharmacology; D002746:Chlorpromazine / Q000494:pharmacology; D004151:Dipeptides / Q000378:metabolism*; D004226:Dithioerythritol / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D066298:In Vitro Techniques; D007460:Iodoacetamide / Q000494:pharmacology; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D010453:Peptide Synthases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology
D002413:Cations, Divalent; D002729:Chloromercuribenzoates; D004151:Dipeptides; D000338:beta-Aminoethyl Isothiourea; D008623:Mercaptoethanol; D004226:Dithioerythritol; D002336:Carnosine; D010453:Peptide Synthases; D000409:Alanine; D004229:Dithiothreitol; D002746:Chlorpromazine; D007460:Iodoacetamide