Purification of urokinase by affinity chromatography.
Commercially available urokinase (EC 3.4.99.26), though highly active, is still contaminated with unrelated proteins and degradation fragments of urokinase. Further purification of a urokinase preparation by chromatography on benzamidine-Sepharose is described. The final preparation consisted of two molecular forms of urokinase with molecular weights of respectively 31 000 and 54 000. The 54 000-dalton urokinase appears to be composed of two protein chains, one of which is the 31 000-dalton urokinase. A monospecific antiserum against urokinase was raised.
D001550:Benzamidines; D002846:Chromatography, Affinity; D010450:Endopeptidases / Q000302:isolation & purification*; D008970:Molecular Weight; D012685:Sepharose; D014568:Urokinase-Type Plasminogen Activator / Q000276:immunology / Q000302:isolation & purification*
D001550:Benzamidines; D012685:Sepharose; D010450:Endopeptidases; D014568:Urokinase-Type Plasminogen Activator