The metabolism of steroids in the fatty liver induced by orotic acid feeding.
1. The metabolism of 4-[4-14C]androstene-3,17-dione, 4-[4-14C]pregnene-3,20-dione, 5alpha-[4-14C]androstane-3alpha,17beta-diol, [4-14C]cholesterol, 7alpha-hydroxy-4-[6beta-3H]cholesten-3-one, 5beta-[7beta-3H]cholestane-3alpha,7alpha-diol and [3H]lithocholic acid was studied in the microsomal fraction of livers from control and orotic acid-treated male rats. 2. As a result of the treatment the orotic acid-fed rats had fatty livers and subnormal concentrations of cholesterol and triglycerides in serum. 3. The 6beta- and 7alpha-hydroxylation of 4-androstene3,17-dione, and the 2alpha-, 2beta- and 18-hydroxylation of 5alpha-androstane-3alpha,17beta-diol, and the 5alpha-reduction of 4-androstene-3,17-dione and 4-pregnene-3,20-dione were decreased by 40--50% in orotic acid-fed rats. Other oxidative and reductive reactions of the steroid hormones were not significantly affected. 4. The 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one was decreased by about 50%, whereas the 7alpha-hydroxylation of cholesterol and the 26-hydroxylation of 5beta-cholestane-3alpha,7alpha-diol were not significantly decreased. The 6beta-hydroxylation of lithocholic acid was stimulated by 40%. 5. The results are discussed in relation to present knowledge of the heapatic drug-metabolizing enzymes and to the recent findings of an abnormal bile acid metabolism in liver disease.
D015113:Androstane-3,17-diol / Q000378:metabolism; D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D002776:Cholestanes / Q000378:metabolism; D002783:Cholestenones / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D004435:Eating; D005234:Fatty Liver / Q000139:chemically induced / Q000378:metabolism*; D008095:Lithocholic Acid / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009963:Orotic Acid; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D013256:Steroids / Q000378:metabolism*; D014280:Triglycerides / Q000032:analysis
D002776:Cholestanes; D002783:Cholestenones; D013256:Steroids; D014280:Triglycerides; D015113:Androstane-3,17-diol; D000735:Androstenedione; D011374:Progesterone; D008095:Lithocholic Acid; D009963:Orotic Acid; D002784:Cholesterol