Decreased levels of androgen binding protein in the reproductive tract of the restricted (Hre) rat.
A simple method was developed for assay of androgen binding protein (ABP) in the reproductive tract of male rats. Dextran-coated charcoal was employed to separate bound and free steroid. The number of binding sites quantified by this technique was the same as those determined by other procedures. The ABP levels in testis and epididymis of Hre rats was much lower than those from normal littermates. This abnormality was correlated with defective spermatogenesis in these animals.
D000375:Aging; D000728:Androgens / Q000032:analysis*; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000032:analysis*; D002606:Charcoal; D003911:Dextrans; D013196:Dihydrotestosterone / Q000378:metabolism; D004822:Epididymis / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D013733:Testicular Diseases / Q000235:genetics; D013737:Testis / Q000378:metabolism*
D000728:Androgens; D002352:Carrier Proteins; D003911:Dextrans; D013196:Dihydrotestosterone; D002606:Charcoal