Substrate metabolism in the isolated perfused dog kidney.
The relationships between substrate utilization and renal work were investigated in an isolated, perfused dog kidney preparation: substrate utilization included free-fatty acid (FFA), glucose, and oxygen, whereas work was assessed by measuring TNa+. The magnitude of TNa+ was varied by altering glomerular filtration rate as a consequence of changing renal blood flow. As the rate of renal blood flow was reduced, both Qo2, and QFFA decreased while Qglucose increased. The reduction in QFFA and Qo2 paralleled the decrease in TNa+. An inverse relationship was noted between QFFA and Qglucose in all experiments. The results suggest that FFA and perhaps glucose may serve as a significant substrate for providing energy for TNA+ in the kidney. Further, since QFFA and Qglucose are inversely related to each other, glucose may substitute for FFA as an energy source at times when QFFA is reduced.
D000818:Animals; D004285:Dogs; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005260:Female; D005919:Glomerular Filtration Rate; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007668:Kidney / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D010101:Oxygen Consumption; D010477:Perfusion; D012039:Regional Blood Flow
D005230:Fatty Acids, Nonesterified; D005947:Glucose