title: Studies on sequencing of peptides from the carboxyl terminus by using the thiocyanate method.
abstract: We report on our experience in applying the thiocyanate method developed by Stark (1968) (Biochemistry 7, 1796-1807) to the sequencing of short peptides from the carboxyl end in free solution. Yields fell to very low levels after three cycles of degradation. The method was time-consuming because of the filtration and freeze-drying stages involved. To overcome these problems, peptides were attached to modified polystyrene polymers for sequential degradation in the solid phase, and a maximum of six amino acids was determined. Also, ribonuclease was attached to active-ester glass beads and sequential degradation was carried out to determine six amino acids at the C-terminal end of this protein.
mesh_terms: D000595:Amino Acid Sequence; D000613:Aminobutyrates; D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D005898:Glass; D009113:Muramidase / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D010455:Peptides / Q000032:analysis*; D011108:Polymers; D012260:Ribonucleases / Q000032:analysis; D013859:Thiocarbamates; D013861:Thiocyanates; D013867:Thiohydantoins / Q000032:analysis
chemical_list: D000613:Aminobutyrates; D010455:Peptides; D011108:Polymers; D013859:Thiocarbamates; D013861:Thiocyanates; D013867:Thiohydantoins; D012260:Ribonucleases; D009113:Muramidase