title: The microdetermination of copper in plasma protein fractions.
abstract: Electrophoresis on cellulose acetate membranes and electrothermal atomization for atomic absorption spectrophotometry have been combined to allow the determination of copper in protein fractions separated from only 2 mul volumes of serum. The sensitivity of the method is 5.2 X 10(-11) g of copper for 1% absorption at 324.7 nm, and the precision of replicate analyses at the 1.7-ng level is 0.086 (Relative Standard deviation). The mean recovery of copper from the alpha2-protein fraction of a serum sample was 94%. The method has been applied to a study of the changes in the copper content of the various protein fractions of sera from patients with Menkes' syndrome.
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D003300:Copper / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D008667:Metalloproteins / Q000097:blood*; D008832:Microchemistry; D013054:Spectrophotometry, Atomic / Q000379:methods
chemical_list: D001798:Blood Proteins; D008667:Metalloproteins; D003300:Copper