Rapid determination of butylated hydroxyanisole, tert- butylhydroquinone, and propyl gallate in edible oils by electron capture gas-liquid chromatography.
A rapid method is described for the determination of 2- and 3-tert-butyl-4-hydroxyanisole (2- and 3-BHA), tert-butylhydroquinone (TBHQ), and propyl gallate (PG) as their heptafluorobutyrates in edible oils. A solution of the oil in ether-benzene is treated with heptafluorobutyric anhydride (HFBA) in the presence of trimethylamine as catalyst. Excess HFBA is destroyed and a suitably diluted aliquot is analyzed by electron capture gas-liquid chromatography. An internal standard is added before derivatization to aid quantitation and identification of the antioxidants. Average recoveries from an oil spiked at 1, 5, 5, and 5 ppm TBHQ, 2-BHA, 3-BHA, and PG were 110, 105, 106, and 102%, respectively. At these low levels, however, some late-eluting peaks interfered. Butylated hydroxytoluene is not determined by this method.
D000840:Anisoles / Q000032:analysis*; D000975:Antioxidants / Q000032:analysis; D002083:Butylated Hydroxyanisole / Q000032:analysis*; D002849:Chromatography, Gas; D005503:Food Additives / Q000032:analysis; D005707:Gallic Acid / Q000031:analogs & derivatives*; D006873:Hydroquinones / Q000032:analysis*; D008722:Methods; D009821:Oils / Q000032:analysis*; D011435:Propyl Gallate / Q000032:analysis*
D000840:Anisoles; D000975:Antioxidants; D005503:Food Additives; D006873:Hydroquinones; D009821:Oils; D002083:Butylated Hydroxyanisole; D005707:Gallic Acid; D011435:Propyl Gallate