Comparative study on the derivatization of l-alpha-acetyl-methadol metabolites for electron capture gas-liquid chromatography.
Six reagents-trichloroacetyl chloride, trichloroacetic anhydride, pentafluorobenzoyl chloride, heptafluorobutyryl chloride, heptafluorobutyric anhydride, and trifluoroacetic anhydride- were evaluated as potential derivatizing reagents for quantitating the metabolites of l-alpha-acetylmethadol (LAAM) -noracetylmethadol, dinoracetylmethadol, methadol, and normethadol-by electron capture gas-liquid chromatography. All of the reagents studied reacted quantitatively with all of the metabolites except methadol; however, trichloroacetyl chloride was found to be the most satisfactory general reagent for analyzing these metabolites in biological fluids. A gas-liquid chromatographic method is presented which combines both flame ionization and electron capture detection for quantitating plasma and urine levels of methadone, l-alpha-acetylmethadol and its metabolites.
D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D006801:Humans; D007202:Indicators and Reagents; D008691:Methadone / Q000031:analogs & derivatives*; D008692:Methadyl Acetate / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine; D008722:Methods
D007202:Indicators and Reagents; D008692:Methadyl Acetate; D008691:Methadone