Phospholipid requirement for dimethylnitrosamine demethylation by hamster hepatic microsomal cytochrome P-450 enzyme system.
Extraction with butan-1-ol of freeze-dried microsomal fractions from livers of 3-methyl-cholarthrene-pre-treated hamsters removed about 90% of the total lipid content, but the lipid remaining proved sufficient for the cytochrome P-450 enzyme system to retain about 15-40% of its original catalytic activity for dimethylnitrosamine demethylation. Addition of butan-1-ol-extracted total phospholipid or phosphatidylcholine could not restore any activity, whereas the addition of the synthetic phospholipid dilauroyl phosphatidylcholine was able to restore almost complete activity. Synthetic dipalmitoyl or distearoyl phosphatidylcholine was ineffective in restoring the activity in this reconstituted system.
D000818:Animals; D000440:Butanols; D006224:Cricetinae; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003579:Cytochrome Reductases / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D008748:Methylcholanthrene; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D009602:Nitrosamines / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D010743:Phospholipids / Q000494:pharmacology
D000440:Butanols; D009602:Nitrosamines; D010713:Phosphatidylcholines; D010743:Phospholipids; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D003579:Cytochrome Reductases; D004128:Dimethylnitrosamine