title: [Modification and immobilization of beta-galactosidase].
abstract: beta-galactosidase from fungus Curvularia inaequalis was modified by a chlortriazin dye active bright-orange KH. The modified enzyme contained two molecules of dye per one molecule of protein. The incorporation of six sulfuric groups with remains of the dye resulted in a slight decrease of the acid protein isoelectric point. The catalytic activity of the modified protein remains practically unchanged. The coloured protein is firmly absorbed on anionites. Preparations of immobilized beta-galactosidase were obtained by adsorption on anionites.
mesh_terms: D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D004396:Coloring Agents; D004800:Enzymes, Immobilized; D005696:Galactosidases; D007525:Isoelectric Focusing; D003904:Mitosporic Fungi / Q000201:enzymology
chemical_list: D004396:Coloring Agents; D004800:Enzymes, Immobilized; D005696:Galactosidases
vernacular_title: Modifitsirovanie i immobilizatsiia beta-galaktozidazy