Bone marrow ribonucleic acid polymerase. Effect of testosterone on nucleotide incorporation into nuclear RNA.
The incorporation of 3H-UTP into RNA by isolated rat bone marrow nuclei is stimulated by testosterone. This effect is hormone and tissue specific. Using alpha-amanitine and different ionic strength conditions it was found that testosterone enhances preferentially RNA polymerase I activity. The sedimentation pattern of RNA isolated from bone marrow nuclei shows that the synthesis of RNA species within the 14-30 S range is mainly stimulated by the hormone.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000201:enzymology*; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005260:Female; D008970:Molecular Weight; D009994:Osmolar Concentration; D012318:RNA Polymerase I / Q000378:metabolism; D051381:Rats; D013739:Testosterone / Q000494:pharmacology*; D014500:Uracil Nucleotides / Q000378:metabolism
D000546:Amanitins; D014500:Uracil Nucleotides; D013739:Testosterone; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I