title: Preparation and storage of platelet concentrates. I. Factors influencing the harvest of viable platelets from whole blood.
abstract: Factors affecting the yield and viability of concentrated platelets have been investigated in a blood component programme. It was found that 86+/-1% of the platelets from a unit of whole blood can be concentrated without loss of viability by processing ACD or CPD anticoagulated blood at room temperature. The steps are initial centrifugation at 1000 g for 9 min to harvest platelet-rich plasma; centrifugation of the platelet-rich plasma at 3000 g for 20 min to pack the platelets; and resuspension of the platelet pellet in residual plasma after 1 1/2 h.
mesh_terms: D001771:Blood Banks / Q000592:standards; D001772:Blood Cell Count; D001792:Blood Platelets; D001793:Blood Preservation; D002469:Cell Separation; D002470:Cell Survival; D002498:Centrifugation; D006801:Humans; D012955:Societies, Medical; D013997:Time Factors; D014481:United States