Synthesis of S-lactoyl-glutathione using glyoxalase I bound to sepharose 4B.
Glyoxalase I bound to Sepharose 4B was used for synthesis of S-lactoyl-glutathione. The bound enzyme does not lose its activity during several months storing and can be used many times for synthesis of S-lactoyl-glutathione. This reaction product can be used as a substrate for glyoxalase II without any further purification.
D004800:Enzymes, Immobilized / Q000378:metabolism*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D007773:Lactates / Q000138:chemical synthesis; D007791:Lactoylglutathione Lyase / Q000378:metabolism*; D008190:Lyases / Q000378:metabolism*; D008722:Methods; D012685:Sepharose; D013869:Thiolester Hydrolases / Q000378:metabolism*; D000099301:Hydroxyacylglutathione Hydrolase
D004800:Enzymes, Immobilized; D005978:Glutathione; D007773:Lactates; D007791:Lactoylglutathione Lyase; D008190:Lyases; D012685:Sepharose; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase