Cytochalasin B-induced triploidy in mouse oocytes fertilized in vitro.
Mouse eggs and spermatozoa were treated in various ways with 5 or 10 mug cytochalasin B/ml. The fertilization rate in vitro was reduced by treatment with the drug but 80-90% of the eggs fertilized were triploid. Many of the experimental eggs were penetrated by one or more spermatozoa but remained unfertilized (75% compared with 9% in control eggs). It is suggested that cytochalasin B weakens the zona reaction and interferes with fusion of gametes but does not prevent the block to polyspermy.
D000818:Animals; D003571:Cytochalasin B / Q000494:pharmacology*; D005260:Female; D005298:Fertility / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009865:Oocytes / Q000187:drug effects* / Q000648:ultrastructure; D010063:Ovum / Q000187:drug effects*; D011123:Polyploidy; D013094:Spermatozoa / Q000187:drug effects; D013480:Superovulation
D003571:Cytochalasin B