Experiments and calculations concerning a thermal enzyme probe.
A simple device capable of measuring almost any reactant in an enzyme-catalyzed reaction is created when an enzyme is immobilized onto one thermal sensor of a differential thermometer. Experiments are described in which two thermistors, one bare and one coated with immobilized enzyme, are immersed in a well-stirred solution. The response of this device to increases in glucose-ATP concentration was observed using hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1), and to increases in glucose concentration using glucose oxidase (beta-D-glucose:oxygen 1-oxidoreductase, EC 1.1.3.4). A simple model is presented whose predictions are in reasonable agreement with the experimental results.
D004800:Enzymes, Immobilized / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D008722:Methods; D008956:Models, Chemical; D013696:Temperature
D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D006593:Hexokinase