Regeneration of renal proximal tubules after mercuric chloride injury is accompanied by increased binding of aminoacyl-transfer ribonucleic acid.
Homogenates of rat kidney cortex obtained 1,3 or 14 days after a single injection of HgCl2 were used to prepare the post-microsomal pH5 supernatant fraction. The activity of this fraction for peptide synthesis from [14C]phenylalanyl-tRNA was significantly increased at 1 and 3 days, at which time the proximal tubules are regenerating [Cuppage & Tate (1967) Am. J. Pathol. 51, 405-429]. This increased activity could not be attributed to a decreased inhibitory activity, but was due to an increased aminoacyl-tRNA binding, i.e. elongation-factor-1 activity, in the supernatant fraction.
D000818:Animals; D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D005672:Fusidic Acid / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000494:pharmacology; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced; D007687:Kidney Tubules, Proximal / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008628:Mercury; D010441:Peptide Chain Elongation, Translational; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D010445:Peptide Elongation Factors; D010448:Peptide Initiation Factors; D010649:Phenylalanine / Q000378:metabolism; D011072:Poly U / Q000494:pharmacology; D012343:RNA, Transfer / Q000378:metabolism; D051381:Rats; D012038:Regeneration; D013997:Time Factors
D010445:Peptide Elongation Factors; D010448:Peptide Initiation Factors; D011072:Poly U; D001312:Aurintricarboxylic Acid; D010649:Phenylalanine; D005672:Fusidic Acid; D006160:Guanosine Triphosphate; D012343:RNA, Transfer; D008628:Mercury