In vitro production of prostaglandins E and F by the guinea pig ovarian tissue.
The ability of guinea pig ovarian tissue to biosynthesize prostaglandins E and F from endogenous precursors has been investigated in vitro. Estimations of prostaglandins were carried out using a sensitive radioimmuno assay during seven days preceding, and up to one day, following oestrous. Prostaglandins E and F were present in the ovarian tissue throughout the period investigated. Prostaglandin concentrations in samples incubated without enzymic inhibition were significantly higher than in samples incubated after enzymic inhibition with ethanol. This indicates that guinea pig ovarian tissue is able to synthesize prostaglandins from endogenous precursors.
D000818:Animals; D004971:Estrus; D005260:Female; D006168:Guinea Pigs; D010053:Ovary / Q000378:metabolism*; D011247:Pregnancy; D011458:Prostaglandins E / Q000096:biosynthesis*; D011460:Prostaglandins F / Q000096:biosynthesis*; D013997:Time Factors
D011458:Prostaglandins E; D011460:Prostaglandins F