Immobilization of enzymes based on hydrophobic interaction. II. Preparation and properties of an amyloglucosidase adsorbate.
Amyloglucosidase from Aspergillus niger (alpha-1,4 and 1,6 glucan glucohydrolase, EC 3.2.1.3) was immobilized through adsorption onto a hexyl-Sepharose, containing 0.51 mol hexyl-group per mole of galactose. The adsporption limit of the carrier with respect to this enzyme was about 17 mg per gram wet conjugate. The retention of activity upon immobilization was high, varying from essentially full activity at low enzyme content down to 68% at the adsorption limit. The immobilized preparation, as well as the soluble enzyme, showed apparent zero order kinetics within 60% of the substrate's conversion limit. Product inhibition of the soluble enzyme showed a KI of 5-10(-2)M. In the presence of 3M NaCl, adsorbates were formed more rapidly and with a higher yield of immobilized protein, but with lower specific activity. Conjugates resulting from adsorption of amyloglucosidase in identical concentrations, but at different salt contents, showed comparable activities and operational stabilities. Continuous operation from three months reduced conjugate activity to 40%. The thermal stability of the adsorbate was inferior to that of the soluble enzyme, but was noticeably enhanced in the presence of substrate.
D000327:Adsorption; D002384:Catalysis; D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D005782:Gels; D005947:Glucose / Q000494:pharmacology; D005959:Glucosidases; D007700:Kinetics; D011134:Polysaccharides; D011506:Proteins / Q000032:analysis; D012685:Sepharose / Q000031:analogs & derivatives; D013696:Temperature; D014867:Water
D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D005782:Gels; D011134:Polysaccharides; D011506:Proteins; D014867:Water; D012685:Sepharose; D005959:Glucosidases; D005947:Glucose