title: Separation of molecular species of glucosylceramide by high performance liquid chromatography of their benzoyl derivatives.
abstract: The method of separation of glucosylceramide by HPLC was reported. Glucosylceramide was perbenzoylated and separated on a packed muBondapack C18 column, using methanol as eluting solvent. The pattern obtained by HPLC closely resembled that obtained by GLC of the TMS-glucosylceramide, and reflected the molecular species of fatty acid components. This method is reproducible, and sensitive as GLC. This method also can be used for analysis of higher glycolipids.
mesh_terms: D001565:Benzoates; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D005776:Gaucher Disease; D006028:Glycosphingolipids / Q000302:isolation & purification*; D006801:Humans; D013154:Spleen
chemical_list: D001565:Benzoates; D005227:Fatty Acids; D006028:Glycosphingolipids