Dimers and trimers of immunoglobulin G covalently cross-linked with a bivalent affinity label.
A bivalent affinity label, bis(alpha-bromoacetyl-epsilon-2,4-dinitrophenyllysylproline)ethylenediamine, has been synthesized. Treatment of anti-2,4-dinitrophenyl antibodies with this compound produces a mixture of covalently and noncovalently cross-linked material. Only specific antibodies are covalently cross-linked, suggesting that covalent attachment occurs in the variable regions. Covalently cross-linked dimers and trimers have been isolated from the reaction mixture in a high state of purity, in yields of about 12 and 4%, respectively. The complexes are stable in solutions containing 10(-4) M hapten and can therefore be used as sensitive probes of immune effector functions.
D000345:Affinity Labels; D000937:Antigen-Antibody Reactions; D004136:Dinitrobenzenes / Q000276:immunology; D005029:Ethylenediamines; D007074:Immunoglobulin G; D009578:Nitrobenzenes / Q000276:immunology; D011108:Polymers
D000345:Affinity Labels; D004136:Dinitrobenzenes; D005029:Ethylenediamines; D007074:Immunoglobulin G; D009578:Nitrobenzenes; D011108:Polymers; C013415:bis(alpha-bromoacetyl-epsilon-2,4-dinitrophenyllysylproline)ethylenediamine