Enzymatic measurement of free and esterified cholesterol levels in plasma and other biological preparations using the oxygen electrode in a modified glucose analyzer.
A method is described for assaying free and esterified cholesterol using the oxygen electrode in a modified glucose analyzer to measure the relative amount of oxygen utilization taking place during oxydation of free cholesterol by the enzyme, cholesterol oxidase. A second enzyme, cholesterol ester hydrolase, is utilized to generate free cholesterol from cholesterol esters. This assay procedure is rapid, specific, reproducible and applicable to the measurement of free and esterified cholesterol carried in the major plasma lipoprotein fractions of man and the rat and, in addition, it can be utilized for the assay of sterols in subcellular fractions of cells.
D000818:Animals; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood; D002788:Cholesterol Esters / Q000032:analysis* / Q000097:blood; D004566:Electrodes; D005947:Glucose; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism; D007700:Kinetics; D008722:Methods; D010101:Oxygen Consumption; D011092:Polyethylene Glycols; D002787:Sterol Esterase / Q000378:metabolism
D002788:Cholesterol Esters; D011092:Polyethylene Glycols; D002784:Cholesterol; D006913:Hydroxysteroid Dehydrogenases; D002787:Sterol Esterase; D005947:Glucose