title: Organisation of genes for ribosomal RNA in Physarum polycephalum.
abstract: Physarum polycephalum nucleolar satellite DNA has been analysed by restriction enzyme digests and hybridisation to ribosomal RNA. The nucleolar DNA is isolated as molecules of molecular weight 39 X 10(6). The positions of sites of endo R-EcoRI and endo R-HindIII digestion have been determined accurately in the whole molecule. The endo R-EcoRI sites and one endo R-HindIII site are within the26-S rRNA complementary sequence and the other endo R-HindIII site is within the 19-S rRNA complementary sequence. The sites are arranged symmetrically about the centre of the molecule as if it were a palindrome.
mesh_terms: D002466:Cell Nucleolus / Q000378:metabolism; D002501:Centrifugation, Zonal; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000378:metabolism; D003851:Deoxyribonucleases; D008970:Molecular Weight; D009235:Myxomycetes / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010804:Physarum / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000096:biosynthesis*
chemical_list: D004276:DNA, Satellite; D012335:RNA, Ribosomal; D004247:DNA; D003851:Deoxyribonucleases; D004262:DNA Restriction Enzymes