[Fluorescent probe study of temperature-dependent conformational transitions in proteins].
Temperature-dependent conformational features of human serum (SAH) and egg albumins were studied by fluorescent probes such as 3-metoxybenzantron (MBA), dimethylaminocholkon (DMC) and 1-anilononaphtalene-8-sulfonate (ANS) in temperature range of 4--68 degrees C. It was found that these proteins undergo the conformational transitions accompanied by the changes of the quantrum yield and maximum position of fluorescence of MBA, DMA and ANS at 12--16 degrees C, 16--23 degrees C, 40--62 degrees C for SAH and at 5--25 degrees C, 46--64 degrees C for egg albumin.
D000817:Anilino Naphthalenesulfonates; D002599:Chalcone / Q000031:analogs & derivatives; D005453:Fluorescence; D006801:Humans; D010047:Ovalbumin; D011487:Protein Conformation; D012709:Serum Albumin; D013696:Temperature
D000817:Anilino Naphthalenesulfonates; D012709:Serum Albumin; D002599:Chalcone; D010047:Ovalbumin
Izuchenie temperaturno-zavisimykh konformatsionnykh perekhodov belkov metodom fluorestsentnykh zondov