title: Influence of glycerol concentration and freezing rate on post-thaw motility of bovine spermatozoa in Continental straws.
abstract: Semen from each of six bulls was used to study the effects of percent glycerol and rate of freezing on spermatozoa extended in egg yolk-sodium citrate extender and packaged in .25-ml Continental straws. Treatments were arranged factorially and included 5, 6, 7, 8, 9, 10, or 11% (volume/volume) glycerol; freezing rates (time from 5 to -130 C) of 1, 2, 3.5, 7, 12, or 20 min; and post-thaw incubation times of 0 and 2 h at 38 C. Post-thaw motility was similar for spermatozoa frozen in 2 to 20 min, and all means were greater than for spermatozoa frozen in 1 min. Post-thaw motility of spermatozoa frozen in 7% glycerol did not differ from that with 5 to 9% but was superior to that with 10 or 11% glycerol. Although glycerol and freezing rate interacted, 7% glycerol provided survival equal to or greater than that with the other percents of glycerol, regardless of freezing rate.
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D003451:Cryoprotective Agents; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology*; D008297:Male; D011309:Preservation, Biological / Q000295:instrumentation / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects*
chemical_list: D003451:Cryoprotective Agents; D005990:Glycerol