An enriched reaction center preparation from green photosynthetic bacteria.
Bacteriochlorophyll a reaction-center complex I from Chlorobium limicola f. thiosulfatophilum 6230 (Tassajara) was incubated in 2 M guanidine - HCl and then chromatographed on cross-linked dextran or agarose gel. Two principal components were separated: a larger component with photochemical activity (bacteriochlorophyll a reaction-center complex II) and a smaller component without activity (bacteriochlorophyll a protein). Complex II contains carotenoid, bacteriochlorophyll a, reaction center(s), and cytochromes b and c, but lacks the well characterized bacteriochlorophyll a protein contained in Complex I. Complex II carries out a light-induced reduction of cytochrome b along with an oxidation of cytochrome c.
D001205:Ascorbic Acid; D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D001429:Bacteriochlorophylls / Q000302:isolation & purification / Q000378:metabolism*; D001665:Binding Sites; D002734:Chlorophyll / Q000031:analogs & derivatives*; D002942:Circular Dichroism; D004227:Dithionite; D006146:Guanidines; D008968:Molecular Conformation; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010788:Photosynthesis; D011485:Protein Binding; D011487:Protein Conformation; D013053:Spectrophotometry
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D006146:Guanidines; D002734:Chlorophyll; D004227:Dithionite; D001205:Ascorbic Acid