Effect of 2-allyl-2-isopropylacetamide on poly(adenylic acid)-containing ribonucleic acid.
A single administration of 2-allyl-2-isopropylacetamide, a porphyrinogenic drug, enhanced the 32P-labelling of nucleoplasmic as well as cytoplasmic poly(A)-containing RNA in rat liver. The synthesis of total microsomal RNA is only marginally increased under these conditions. The drug enhances the labelling of a variety of cytoplasmic poly(A)-containing RNA species, and this effect is counteracted by the simultaneous administration of haemin. 2-Allyl-2-isopropylacetamide also enhanced the release of RNA from the nucleus to the cytoplasm.
D000081:Acetamides / Q000494:pharmacology*; D000502:Allylisopropylacetamide / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006427:Hemin / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011061:Poly A; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D013217:Starvation
D000081:Acetamides; D010761:Phosphorus Radioisotopes; D011061:Poly A; D000502:Allylisopropylacetamide; D012313:RNA; D006427:Hemin