[Gaschromatographic determination of pramiverine in body fluids (author's transl)].
A method for the quantitative determination of 4,4-diphenyl-N-isopropyl-cyclohexylamine-hydrochloride (pramiverine, Sistalgin) in serum and urine was developed. After the addition of a known amount of a pramiverine homolog as an internal standard the basic pramiverine and standard are separated from acidic and neutral constituents of the sample by the following extraction sequence: Extraction from the alkalized sample into n-hexane/ethyl acetate; reextraction into 0.1 N hydrochloric acid; alkalisation of the hydrochloric acid phase and reextraction into n-hexane/ethyl acetate. The constituents of this final extract are concentrated and separated on an OV 17 silicon oil phase. A thermoionic nitrogen-selective flame ionisation detector was used. The determination limit for 5-ml serum samples is about 0.5-1 ng pramiverine/ml. For samples in the concentration range of 4 ng/ml a relative standard deviation of 12% was found.
D001559:Benzhydryl Compounds / Q000032:analysis; D002849:Chromatography, Gas; D003514:Cyclohexylamines / Q000032:analysis*; D008722:Methods; D010276:Parasympatholytics / Q000032:analysis*
D001559:Benzhydryl Compounds; D003514:Cyclohexylamines; D010276:Parasympatholytics
Gaschromatographische Bestimmung von Pramiverin in Körperflüssigkeiten