title: Effect of alfalfa fiber substrate on culture counts of rumen bacteria.
abstract: A medium has been developed using alfalfa fiber as the sole substrate. It gave high culture counts (3 X 10(9) to 8 X 10(9)/ml) of rumen bacteria. When this medium was combined with the medium 98-5 of Bryant and Robinson, modified to contain 33% rumen fluid instead of 40% clarified rumen fluid, a higher count was obtained than with either medium alone.
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D002241:Carbohydrates; D002417:Cattle; D003470:Culture Media; D005750:Gastric Juice; D000455:Medicago sativa; D012417:Rumen / Q000382:microbiology*; D014908:Triticum
chemical_list: D002241:Carbohydrates; D003470:Culture Media