Fusion and protein-mediated phospholipid exchange studied with single bilayer phosphatidylcholine vesicles of different density.
A novel method has been developed for the study of phospholipid exchange and fusion of phospholipid vesicles. Two homogeneous populations of single bilayer phosphatidylcholine vesicles of similar size but markedly different density have been prepared. "Dense" vesicles were made from brominated dioleoyl phosphatidylcholine. "Light" vesicles were prepared from dioleoyl phosphatidylcholine. The two populations were easily separated by density gradient centrifugation. Phosphatidylcholine exchange protein from beef liver was used to promote lecithin exchange between the vesicle populations. Only the lecithin of the external monolayers of the vesicles was available for exchange by exchange protein, implying that flip-flop of vesicle phosphatidylcholine did not take place at a detectable frequency. No spontaneous intervesicle phosphatidylcholine exchange was observed. However, the dense and light vesicles did spontaneously fuse, over several hours, to produce particles of hybrid density.
D001692:Biological Transport; D007700:Kinetics; D008433:Mathematics; D008567:Membranes, Artificial; D008954:Models, Biological; D010713:Phosphatidylcholines; D011506:Proteins
D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D011506:Proteins