Rapid and intensive conversion of 5alpha-androstane-3alpha, 17beta-diol into 5alpha-dihydrotestosterone in the male rat anterior pituitary: in vivo and in vitro studies.
The in vivo and in vitro metabolism of (3H)-5alpha-androstane-3lapha,17beta-diol by the male rat anterior pituitary was studied. A rapid and intensive conversion of 5alpha-androstane-3alpha, 17beta-diol into 5alpha-dihydrotestosterone was demonstrated, since following a 30 min. incubation time, 73% of the recovered radioactivity were constituted by 5alpha-dihydrotestosterone. Studies on the subcellular distribution of steroids showed that 5alpha-dihydrotestosterone was the main steroid recovered except from the 105,000 X g pellet. From in vivo and in vitro experiments it was concluded that the transformation of 5alpha-dihydrotestosterone into 5alpha-androstane-3alpha, 17beta-diol was a reversible process, and that this last steroid could exert its biological action mainly via 5alpha-dihydrotestosterone.
D000728:Androgens / Q000302:isolation & purification / Q000378:metabolism; D015113:Androstane-3,17-diol / Q000378:metabolism*; D000731:Androstanes / Q000378:metabolism*; D000818:Animals; D003460:Crystallization; D013196:Dihydrotestosterone / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D013329:Structure-Activity Relationship
D000728:Androgens; D000731:Androstanes; D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol