[Investigation of electrophoretical enzyme phänotypes glucose-6-phosphate dehydrogenase, red cell acid phosphatase, phosphoglucomutase and adenosine deaminase of permanent human cell lines (author's transl)].
Isoenzymes of 11 cell lines were investigated by electrophoretical separation. All lines have been cultivated from tissues of white persons, all but one (Leuc. Th. B.) were phosphoglucomutase 1 and all were adenosine deaminase 1. Three out of 11 cell lines did show glucose-6-phosphate dehydrogenase (G6PD) type B as expected. Two out of 8 cell lines with G6PD A were distinguishable by their specific type of "red cell" acid phosphatase (SEP). We conclude that in vitro the electrophoretical G6PD phänotyp B changed to phänotype A. Further 4 lines had other peculiarities which are indicative to their originality, though they were G6PD A. Our investigations did show that G6PD may become type A if a cell line changes to permanent growth capacity in vitro. The enzyme marker G6PD A alone may not be valuated as an absolute evidence for contamination or mix up with He-La Cells.
D000135:Acid Phosphatase / Q000097:blood*; D000243:Adenosine Deaminase / Q000097:blood; D002460:Cell Line; D004912:Erythrocytes / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D006367:HeLa Cells / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000097:blood; D010641:Phenotype; D010733:Phosphoglucomutase / Q000097:blood; D013997:Time Factors
D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase; D000135:Acid Phosphatase; D000243:Adenosine Deaminase; D010733:Phosphoglucomutase
Untersuchung der elektrophoretischen Enzymphänotypen Glukose-6-Phosphatdehydrogenase, Saure Erythrozytenphosphatase, Phosphoglukomutase und Adenosindesaminase von 11 permanenten Menschzellinien