Measurement of enzymatic activities in turbid suspensions by double beam spectrophotometry.
A method for the determination of enzyme activities in turbid suspension is described, based on the use of a double beam or double wavelength spectrophotometer in one or two cell mode. It measures the disappearance of NADH, coupled to the activity of the enzyme, at a 340 wavelength or at a 340 minus 374 mmu wavelength pair. The method has been applied to the determination of pyruvate kinase activity, in the presence of isolated mitochondria, and other possible applications are discussed.
D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D008930:Mitochondria, Liver / Q000201:enzymology*; D009535:Nicotinamidase / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000378:metabolism*; D051381:Rats; D013053:Spectrophotometry / Q000379:methods
D011770:Pyruvate Kinase; D000581:Amidohydrolases; D009535:Nicotinamidase