A novel method for the rapid quantitative determination of ribosome dissociation factor (IF-3) activity. An assay based on the coupling of the dissociation and peptidyltransferase reactions.
D000818:Animals; D001665:Binding Sites; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D010458:Peptidyl Transferases / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism* / Q000648:ultrastructure; D011691:Puromycin / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D051381:Rats; D012269:Ribosomal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism* / Q000648:ultrastructure
D012269:Ribosomal Proteins; D011691:Puromycin; D012343:RNA, Transfer; D010458:Peptidyl Transferases