Development of a short term assay for lymphoblastic transformation in man.
Blastic stimulation of lymphocytes by antigens or mitogens is usually measured by 3H-TdR labelling of DNA synthesis in 4 to 7 days. However, it has been recently shown, first in mice and guinea pigs, then in men, that it is possible to detect an earlier event by measurement of protein synthesis with 3H-Leucine in a few hours. The work presented here is devoted to determine the optimal conditions of such a short term assay in man. The technique we propose is described. Preliminary clinical results in lymphomas patients, compared to "in vitro" skin tests, are encouraging.
D001943:Breast Neoplasms / Q000276:immunology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007553:Isotope Labeling; D037102:Lectins / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism*; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000276:immunology; D008722:Methods; D014176:Protein Biosynthesis; D013997:Time Factors
D037102:Lectins; D007930:Leucine