title: Developmental specificity and evolution of the acid phosphatase isozymes of Triticum aestivum and its progenitor species.
abstract: The tissue and developmental specificities of the acid phosphatase (ACPH) isozymes of Triticum aestivum and its progenitor species T. turgidum and T. tauschii have been determined and compared using the zymogram technique. Tissue and/or developmental variation in relative staining intensity, suggestive of variation in the quantity of active enzyme present, was observed for each of the seven major isozymes expressed. Isozymes homologous to each of the major isozymes of the hexaploid were detected in one or the other of the progenitor species. No difference in the pattern of developmental or tissue specificity was observed between the species for any isozyme. However, ACPH-4, encoded by ACph4, a structural gene linked to chromosome 4A, differs in electrophoretic mobility between T. aestivum and T. turgidum, indicating that divergence has occurred between these species at the Acph4 locus since the origin of the hexaploid. The molecular weight of each of five ACPH isozymes of the hexaploid was determined to be approximately 58,000. This finding, plus the results of the developmental study and the earlier demonstration that the structural genes for six isozymes (includinomosomes, provides evidence in support of the suggestion that the ACPH structural genes of hexaploid wheat are homoeologous related.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D005075:Biological Evolution; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D014644:Genetic Variation; D007527:Isoenzymes / Q000378:metabolism*; D008970:Molecular Weight; D010641:Phenotype; D010944:Plants / Q000201:enzymology*; D013045:Species Specificity; D014908:Triticum / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D000135:Acid Phosphatase