The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase.
Mild alkaline treatment of glycopeptide (GP-II) resulted in the loss of 1 mole of serine and 5 moles of threonine per mole of GP-II, suggesting the presence of O-glycosyl bonds between 1 serine and 5 threonine residues and carbohydrate chains. Treatment of GP-II with alkaline borohydride released only disaccharide. Methylation studies of the carbohydrate moiety gave 2,3,4,6-tetra-O-methyl and 2,4,6-tri-O-methyl derivatives of mannose in a ratio of approximately 1:1. In addition, one step of Smith degradation resulted in the loss of about 6 residues of mannose per mole of GP-II. Moreover, alpha-mannosidase [EC 3.2.1.24] liberated about 6 residles of mannose per mole of GP-II. On the basis of these data, the structure of the carbohydrate moiety of GP-II was confirmed to be 3-O-alpha-mannosylmannose. The amino- and carboxyl-terminal amino acids of GP-II were determined to be threonine and serine, respectively. On reductive cleavage of N-proline bonds with metallic sodium in liquid ammonia,  2 moles of alanine per mole of GP-II were lost. From the compositions of three fragments isolated from the reductive cleavage products, the amino acid sequence of the peptide portion of GP-II was determined. Based on these data, a probable structure was proposed for GP-II.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000681:Amylases; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D006020:Glycopeptides; D006026:Glycoside Hydrolases; D006868:Hydrolysis; D007700:Kinetics; D012233:Rhizopus / Q000201:enzymology*
D000596:Amino Acids; D002241:Carbohydrates; D006020:Glycopeptides; D000681:Amylases; D006026:Glycoside Hydrolases