Purification of plasma membranes from rat mammary gland by a density perturbation procedure.
A highly purified plasma membrane fraction was obtained from a microsomal subfraction of rat mammary gland after treatment with digitonin to increase its density. The purified membranes were enriched 70-fold overall in 5'-nucleotidase with essentially no contamination from glactosyltransferase, succinate-ING reductase, or NADPH-cytochrome c reductase. The membranes were also highly enriched in sialoglycoproteins.
D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D004072:Digitonin; D005260:Female; D008321:Mammary Glands, Animal / Q000648:ultrastructure*; D009708:Nucleotidases / Q000032:analysis; D051381:Rats; D012795:Sialoglycoproteins / Q000032:analysis
D012795:Sialoglycoproteins; D009708:Nucleotidases; D004072:Digitonin