The oxidation of terminal D-galactofuranose residues of a galactan and a glycoprotein by a D-galactose oxidase preparation from Dactylium dendroides.
A galactan, isolated from the unicellular organism Prototheca zopfii, and a glycoprotein from a hyphal cell-wall fraction of the fungus Pithomyces chartarum have been oxidised by a D-galactose oxidase preparation from Dactylium dendroides. The oxidised polymers were subsequently reduced with sodium borotritide. The site of oxidation was identified as C-6 of non-reducing D-galactofuranosyl residues in both polymers.
D000429:Alcohol Oxidoreductases; D005690:Galactose / Q000032:analysis*; D005692:Galactose Oxidase; D006023:Glycoproteins; D008722:Methods; D003904:Mitosporic Fungi / Q000201:enzymology; D010084:Oxidation-Reduction; D011134:Polysaccharides
D006023:Glycoproteins; D011134:Polysaccharides; D000429:Alcohol Oxidoreductases; D005692:Galactose Oxidase; D005690:Galactose