Steroid C-21-hydroxylation by dog adrenal microsomes.
1,2-3H121-Deoxycortisol (1) 1,2-3H21-deoxycortisone and 1,2-3H-17OH-progesterone were incubated separately with dog adrenal microsomes. With substrates concentrations of 30 and 150 nmoles all the substrates were hydroxylated at C-21 to a similar extent. This result suggests that 21-deoxycortisol could be an intermediate in the biosynthesis of cortisol provided it is avilable in vivo.
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D003348:Cortisone / Q000096:biosynthesis; D004285:Dogs; D005260:Female; D006854:Hydrocortisone / Q000096:biosynthesis; D006889:Hydroxycorticosteroids / Q000378:metabolism*; D006908:Hydroxyprogesterones / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism*
D006889:Hydroxycorticosteroids; D006908:Hydroxyprogesterones; D013250:Steroid Hydroxylases; D003348:Cortisone; D006854:Hydrocortisone