title: Formiminotransferase cyclodeaminase from porcine liver. An octomeric enzyme containing bifunctional polypeptides.
abstract: The forminiminotransferase-cyclodeaminase complex contains 2.8% carbohydrate and is composed of identical subunits based on molecular weight, isoelectric focusing and the number of CNBr-generated peptides. Electron microscopy demonstrated that the complex is a ring-shaped molecule when negatively stained both in solution and in crystalline structures. Rotational reinforcement of electron micrographs reveals that the molecule is composed of eight subunits arranged in a planar ring. These results indicate that each polypeptide of the complex must contain both enzymic activities.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000642:Ammonia-Lyases; D000818:Animals; D001665:Binding Sites; D003488:Cyanogen Bromide; D005565:Formiminoglutamic Acid; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008970:Molecular Weight; D009097:Multienzyme Complexes; D010446:Peptide Fragments; D011485:Protein Binding; D011487:Protein Conformation; D013763:Tetrahydrofolates; D014166:Transferases
chemical_list: D000596:Amino Acids; D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D010446:Peptide Fragments; D013763:Tetrahydrofolates; D005565:Formiminoglutamic Acid; D014166:Transferases; D000642:Ammonia-Lyases; D003488:Cyanogen Bromide