Globin synthesis in normal human bone marrow.
Globin synthesis has been studied by in vitro labelling with radioactive amino acids in 60 normal human bone-marrow samples. Under the conditions routinely used to fractionate alpha and beta chains by chromatography alpha/beta production ratios ranging from 0.5 to 1.0 were obtained, depending on the method of sample treatment. This variation was due entirely to the presence of non-haem proteins derived from white cells which chromagraphy with globin on CM-cellulose. Purification of globin on Sephadex G100 and fractionation of alpha and beta globin chains by a modified chromatographic system resulted in alpha/beta ratios of unity. The relevance of these findings to the study of marrows in which there is unbalanced globin chain production is discussed.
D001798:Blood Proteins / Q000494:pharmacology; D001853:Bone Marrow / Q000032:analysis*; D002498:Centrifugation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003911:Dextrans / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000378:metabolism; D005914:Globins / Q000096:biosynthesis* / Q000302:isolation & purification; D006801:Humans; D007532:Isoleucine / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D001798:Blood Proteins; D003911:Dextrans; D007532:Isoleucine; D014316:Tritium; D005914:Globins; D008239:Lysine