Effects of alpha-chlorohydrin on the metabolism of testicular and epididymal spermatozoa of rams.
Spermatozoa were collected from the rete testis and vas deferens of conscious rams. The endogenous oxygen uptake of the spermatozoa was unaffected by alpha-chlorohydrin added in vitro, although this compound abolished the stimulation of oxygen uptake caused by the addition of glycerol. The metabolism of [14C]glycerol by testicular and epididymal spermatozoa was markedly reduced by alpha-chlorohydrin, CO2 production and lactate accumulation being almost totally inhibited. These effects were dependent upon a period of preincubation of the spermatozoa with alpha-chlorohydrin alone, since the presence of glycerol protected the spermatozoa from its action. Longer exposure and a higher concentration of alpha-chlorohydrin were needed with testicular than with epididymal spermatozoa to achieve a maximal effect. The metabolism of [14C]glucose by both sperm types was also inhibited by alpha-chlorohyrin. Spermatozoa of the ram are therefore susceptible to the action of alpha-chlorohydrin throughout the epididymis, although more mature spermatozoa are more affected. It is suggested that alpha-chlorohydrin is converted to an intermediate which is the agent responsible for the inhibition of glycolysis in spermatozoa.
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002728:Chlorohydrins / Q000494:pharmacology*; D003864:Depression, Chemical; D004822:Epididymis; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D012152:Rete Testis; D012756:Sheep / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D000517:alpha-Chlorohydrin / Q000494:pharmacology*
D002728:Chlorohydrins; D007773:Lactates; D002245:Carbon Dioxide; D000517:alpha-Chlorohydrin; D005947:Glucose; D005990:Glycerol