Use of a cholesterol-rich bovine lipoprotein to enhance cholesterol concentrations in the preparation of serum control materials.
We describe a process for preparing a cholesterol-rich lipoprotein extract from bovine serum. The material is used to enhance cholestrol concentration up to 3.5 g/liter in a clear control material from human serum. The optical clarity of the enriched serum is only slightly affected by lyophilization and reconstitution. The lipoprotein additive neither produces any evidnet interference in the measurement of the commonly analyzed consitutents nor affects the stability of components after lyophilization and reconstitution.
D000818:Animals; D001769:Blood; D001798:Blood Proteins / Q000032:analysis; D002118:Calcium / Q000097:blood; D002417:Cattle; D002784:Cholesterol / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood* / Q000494:pharmacology; D008722:Methods; D009994:Osmolar Concentration; D011786:Quality Control; D012709:Serum Albumin / Q000032:analysis; D014280:Triglycerides / Q000097:blood
D001798:Blood Proteins; D008055:Lipids; D008074:Lipoproteins; D012709:Serum Albumin; D014280:Triglycerides; D002784:Cholesterol; D002118:Calcium