Nuclease cleavage of chromatin at 100-nucleotide pair intervals.
Digestion of mouse liver nuclei with DNase II leads to a novel cleavage pattern with a 100-nucleotide pair periodicity. From chromatin, this pattern or the standard 200-nucleotide pair repeat can be produced depending on the ionic conditions. The results are interpreted by assuming different conformational states of the nuclear material, including condensed and extended forms.
D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000648:ultrastructure*; D003851:Deoxyribonucleases / Q000378:metabolism*; D003852:Deoxyribonucleoproteins / Q000032:analysis; D006657:Histones / Q000378:metabolism; D008099:Liver / Q000648:ultrastructure; D051379:Mice; D008836:Micrococcal Nuclease / Q000378:metabolism; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D011487:Protein Conformation; D013329:Structure-Activity Relationship
D002843:Chromatin; D003852:Deoxyribonucleoproteins; D006657:Histones; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease