Histochemical studies of delta5-3beta-, 20lapha- and 20beta-hydroxysteroid dehydrogenases and possible progestagen production in hamster eggs.
Hamster eggs at various developmental stages were studied histochemically fro the presence of delta5-3beta-hydroxysteroid dehydrogenase (HSD) (pregnenolone as the substrate and NAD as the cofactor), 20alpha-HSD (20alpha-hydroxypregn-4-en-3-one and NADP) and 20 beta-HSD (20beta-hydroxypregn-4-en-3-one and NAD). All three enzymes were absent from ovarian eggs but were demonstrated in unfertilized and fertilized eggs up to the blastocyst stage. The activities varied only slightly.
D000818:Animals; D001755:Blastocyst / Q000201:enzymology; D003349:Cortisone Reductase / Q000378:metabolism; D006224:Cricetinae; D005260:Female; D005306:Fertilization; D006651:Histocytochemistry; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D010063:Ovum / Q000201:enzymology*; D011375:Progesterone Reductase / Q000378:metabolism
D006913:Hydroxysteroid Dehydrogenases; D011375:Progesterone Reductase; D003349:Cortisone Reductase