Determination of serum nucleotidase with cytidine monophosphate as substrate. Part II: Improvement of the procedure.
Serum mucleotidase activity (EC 3.1.3.5) is measured by the amount of ammonia liberated from cytidine after incubation with cytidine deaminase (EC 3.5 4.5). Purification of the cytidine deaminase results in the abolition of interfering reactions, so that routine application is possible.
D000641:Ammonia; D003564:Cytidine Deaminase / Q000302:isolation & purification; D003597:Cytosine Nucleotides; D006801:Humans; D009708:Nucleotidases / Q000097:blood*; D013053:Spectrophotometry / Q000379:methods
D003597:Cytosine Nucleotides; D000641:Ammonia; D009708:Nucleotidases; D003564:Cytidine Deaminase