Regulation of RNA synthesis in corpora lutea nucleic by luteinizing hormone and derivatives.
Luteinizing hormone (lutropin) stimulates glucose-6-phosphate dehydrogenase (EC 1.1.1.49) in isolated nuclei obtained from cow corpus luteum tissue. This increases the biosynthesis de novo of purine and pyrimidine nucleotides and their incorporation into RNA. RNA synthesis was measured after nuclei were incubated for 10 min in buffer media. Several reduction cleavage products and tryptic digests of lutropin inhibited the stimulatory effect of lutropin on RNA synthesis. A site of this inhibition was glucose-6-dehydrogenase.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002918:Chymotrypsin; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005958:Glucosephosphates / Q000378:metabolism; D007986:Luteinizing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D009963:Orotic Acid / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology; D011247:Pregnancy; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic / Q000187:drug effects*; D014357:Trypsin
D005958:Glucosephosphates; D010446:Peptide Fragments; D009963:Orotic Acid; D007986:Luteinizing Hormone; D005954:Glucosephosphate Dehydrogenase; D002918:Chymotrypsin; D014357:Trypsin