title: A study of cow's milk containing high levels of linoleic acid: isolation and properties of the fat-globule membrane.
abstract: Properties of whole milk and milk fractions from cows fed a diet that gave a greatly increased proportion of unsaturated fatty acid residues (especially of linoleic acid) in the milk lipids were studied, and this milk (high-linoleic milk) was compared with milk from cows on a control diet (control milk). The milk fractions were isolated by high-speed centrifugation of whole milk or cream and were examined by chemical analysis and electron microscopy. During centrifugation the globules of milk fat were disrupted and the membranes (fat-globule 'ghosts') floated as a layer beneath the free lipid. Membrane proteins from the 2 sorts of milk gave the same electrophoretic pattern and the amino acid compositions were the same. Lipid analysis of the membrane fraction from high-linoleic milk showed the expected increase in the proportion of unsaturated fatty acid residues in the neutral lipids, but there was an unexpected decrease in the proportion of unsaturated residues in the membrane phospholipids. No differences were found between high-linoleic and control milk in the ultrastructure of the milk-fat globules or the isolated membranes.
mesh_terms: D000596:Amino Acids; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004041:Dietary Fats; D005227:Fatty Acids; D005260:Female; D008041:Linoleic Acids; D008055:Lipids / Q000032:analysis; D008563:Membrane Lipids; D008565:Membrane Proteins / Q000302:isolation & purification; D008566:Membranes / Q000032:analysis; D008854:Microscopy, Electron; D008892:Milk / Q000032:analysis / Q000166:cytology; D008894:Milk Proteins; D014461:Ultracentrifugation
chemical_list: D000596:Amino Acids; D004041:Dietary Fats; D005227:Fatty Acids; D008041:Linoleic Acids; D008055:Lipids; D008563:Membrane Lipids; D008565:Membrane Proteins; D008894:Milk Proteins