The transient-state kinetics of two-substrate enzyme systems operating by an ordered ternary-complex mechanism.
Transient-state kinetic equations, applicable to single-turnover experiments with limiting amounts of substrate and catalytic-suicide experiments with substrates in excess to enzyme, have been derived for two-substrate enzyme reactions occurring by an ordered ternary-complex mechanism. The relative magnitude and dependence on substrate concentrations of rates and amplitudes for the exponential transients corresponding to this mechanism. It is shown that ordered ternary-complex reactions under standard assay conditions may exhibit a biphasic transient rate behaviour, and the precise conditions under which two transients can be detected are described and discussed.
D001665:Binding Sites; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D008722:Methods; D011485:Protein Binding
D004798:Enzymes