Simultaneous determination of neutral sugars and hexosamines in glycoproteins and acid mucopolysaccharides (glycosaminoglycans) by gas-liquid chromatography.
A reliable and reproducible method for the simultaneous determination of neutral sugars and hexosamines in glycoproteins and acid mucopolysaccharides has been described. It involves the following steps: the release of neutral sugars and hexosamines from biopolymers by resin-catalysed hydrolysis, the nitrous acid deamination of resin-bound hexosamines in this hydrolysate to aldoses, and the determination of these newly formed anhydroaldoses together with the pre-existing neutral sugars as aldononitrile acetates by gas chromatography. Applications of this method to analyses of glycoprotein from urine of schizophrenic patients, bovine thyroglobulin, human brain glycoprotein, chondroitin sulfates from bovine vitreous humor, and veal brain, and human umbilical cord hyaluronic acid are given.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002809:Chondroitin Sulfates / Q000032:analysis; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D006023:Glycoproteins / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis*; D006595:Hexosamines / Q000032:analysis*; D006601:Hexoses / Q000032:analysis*; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis; D008722:Methods; D012559:Schizophrenia / Q000652:urine; D014470:Umbilical Cord / Q000032:analysis; D014822:Vitreous Body / Q000032:analysis
D006023:Glycoproteins; D006025:Glycosaminoglycans; D006595:Hexosamines; D006601:Hexoses; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates