The proliferative status of intestinal epithelial clonogenic cells: sensitivity to S phase specific cytotoxic agents.
High concentrations of tritiated thymidine and cytosine arabinoside (Ara-C) have been used to selectively kill cells in the crypts of Lieberkuhn that are synthesizing DNA. The effect of these agents on the number of regenerating microcolonies seen 3 1/2 days after a range of radiation doses indicates that a majority of the clonogenic cells are proliferating rapidly and that the slowly proliferating cells at the base of the crypt do not represent the whole clonogenic population.
D000818:Animals; D002455:Cell Division / Q000187:drug effects* / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects; D003561:Cytarabine / Q000494:pharmacology*; D005260:Female; D005720:Gamma Rays; D007413:Intestinal Mucosa / Q000166:cytology* / Q000187:drug effects; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D013936:Thymidine / Q000494:pharmacology*; D014316:Tritium
D003561:Cytarabine; D014316:Tritium; D013936:Thymidine