alpha1-Microglobulin, a new low molecular weight plasma protein.
A new low molecular weight protein was purified from the urine of uraemic patients. The protein was found to be glycoprotein with a molecular weight of 31 500, determined by SDS-polyacrylamide gel electrophoresis, and a carbohydrate content of 19%. It was electrophoretically heterogenous and migrated in the slow alpha1-region. The mean serum level in 20 apparently healthy individuals was 32+/-10 mg/1. The serum level was normal in 5 patients with severe reduction of plasma protein synthesis caused by cirrhosis of the liver but elevated in patients with a decreased glomerular filtration rate. The mean urinary excretion in 5 healthy individuals was 1.3 mg/24 h. Increased excretion was seen in 10 patients with varying degrees of uraemia.
D000596:Amino Acids / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005919:Glomerular Filtration Rate; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007674:Kidney Diseases / Q000097:blood; D008103:Liver Cirrhosis / Q000097:blood; D008970:Molecular Weight; D012712:Serum Globulins / Q000378:metabolism* / Q000652:urine; D012794:Sialic Acids / Q000032:analysis; D014511:Uremia / Q000097:blood / Q000378:metabolism* / Q000652:urine
D000596:Amino Acids; D006595:Hexosamines; D006601:Hexoses; D012712:Serum Globulins; D012794:Sialic Acids