title: Characterization of ADP-glucose pyrophosphorylase from shrunken-2 and brittle-2 mutants of maize.
abstract: Electrophoretic characterization of adenosine diphosphate glucose pyrophosphorylase from the developing endosperms of nine shrunken-2 and four brittle-2 mutants revealed that (1) all mutants had low but detectable levels of activity, (2) mutation at either locus decreased activity of pyrophosphorylases A and B, and (3) differences in mobility were not found. However, pyrophosphorylase B extracted from several shrunken-2 and brittle-2 mutants differed from normal in extent of urea denaturation, Km (glucose-1-phosphate) or type of glucose-1-phosphaociation with the sh2 locus) appears to differ from normal in Km (glucose-1-phosphate).
mesh_terms: D000245:Adenosine Diphosphate Glucose; D000483:Alleles; D005838:Genotype; D006824:Hybridization, Genetic; D007700:Kinetics; D009154:Mutation; D009713:Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*; D013045:Species Specificity; D014508:Urea; D003313:Zea mays / Q000201:enzymology
chemical_list: D000245:Adenosine Diphosphate Glucose; D014508:Urea; D009713:Nucleotidyltransferases