Separation of molecular species of glucosylceramide by high performance liquid chromatography of their benzoyl derivatives.
The method of separation of glucosylceramide by HPLC was reported. Glucosylceramide was perbenzoylated and separated on a packed muBondapack C18 column, using methanol as eluting solvent. The pattern obtained by HPLC closely resembled that obtained by GLC of the TMS-glucosylceramide, and reflected the molecular species of fatty acid components. This method is reproducible, and sensitive as GLC. This method also can be used for analysis of higher glycolipids.
D001565:Benzoates; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D005776:Gaucher Disease; D006028:Glycosphingolipids / Q000302:isolation & purification*; D006801:Humans; D013154:Spleen
D001565:Benzoates; D005227:Fatty Acids; D006028:Glycosphingolipids