Alteration by malignant transformation of the calcium requirements for cell proliferation in vitro.
Cells from the thigh muscles of normal fetal rats proliferated rapidly and indefinitely in a medium containing adult rat "plasma" and a normal free-calcium concentration, but they could not proliferate in calcium-deficient plasma medium. As the animals grew older, the cells became increasingly less able to proliferate even in normal (high-calcium) plasma medium, though they retained the potential to proliferate in a more conventional medium containing fetal bovine serum. By contrast, neoplastic adult cells from malignant rhabdomyosarcomas (induced by Ni3S2) proliferated rapidly and indefinitely in both normal and low-calcium plasma medium
D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005333:Fetus / Q000378:metabolism; D008297:Male; D009374:Neoplasms, Experimental / Q000378:metabolism; D051381:Rats; D012208:Rhabdomyosarcoma / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D004273:DNA, Neoplasm; D004247:DNA; D002118:Calcium