Determined of bufuralol and its metabolites in plasma by mass fragmentography and by gas chromatography with electron capture detection.
A sensitive method for the simulataneous determination of bufuralol and its pharmacologically active metabolites in huamn plasma is described. The O-timethylsilyl,N-trifluoroacetyl derivatives are assayed by mass fragmentography. Sensitivity is 1 ng 76(-1) plasma for bufaralol and about 250 pg ml(-1) for the metabolites. An alternative procedure which uses gas chromatography with electron capture detection is also described. The sensitivity of this is about 10 ng ml(-1) plasma for all drug-related components.
D001572:Benzofurans / Q000097:blood / Q000494:pharmacology; D002849:Chromatography, Gas / Q000379:methods*; D004583:Electrons; D004983:Ethanolamines / Q000097:blood* / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D066298:In Vitro Techniques; D007659:Ketones / Q000097:blood; D013058:Mass Spectrometry; D010636:Phenols / Q000097:blood
D001572:Benzofurans; D004983:Ethanolamines; D007659:Ketones; D010636:Phenols; C010831:bufuralol