[Electron microscopic localization of serotonin acetyl transferase in the pineal body].
Substrat of the hydroxy-indol-O-methyl transferase (HIOMT) N-acetylserotonin is synthesized by serotonin-acetyltransferase. For the demonstration of the localization of this enzyme in the corpus pineale uranyl and cuprum ferrocyanid technique were used. In both cases a mitochondrial localization was revealed. Product of reaction appeared on the outer and inner membranes and in the intracrystal space. The reaction takes place in the presence of Acetyl-Co-A. The cuprumferrocyanid technique seems to have advantages as compared to the uranylferrocyanid method. Extramitochondrial precipitate or aspecific reaction occur seldom.
D000121:Acetylserotonin O-Methyltransferase / Q000032:analysis*; D000818:Animals; D005260:Female; D008297:Male; D008780:Methyltransferases / Q000032:analysis*; D010870:Pineal Gland / Q000201:enzymology*; D051381:Rats
D008780:Methyltransferases; D000121:Acetylserotonin O-Methyltransferase
Szerotoninacetil-transzferáz elektronmikroszkópos lokalizációja corpus pinealeban