[In vitro incorporation of (33P)orthophosphate into the erythrocyte membrane phospholipids of newborn and adult domestic pigs].
Phosphate turnover of erythrocyte membrane phospholipids, judged by [33P] incorporation, was investigated with phosphatidylethanolamine, lecithin, sphingomyelin, phosphatidylserine, phosphatidylinositol, and phosphatidic acid in newborn and adult domestic pigs. A new micromethod is described in detail using between 10(9) to 10(10) cells, both for quantitative determination of phospholipids and tracer incorporation studies in erythrocyte membrane phospholipids. Erythrocyte phospholipid values are given for pigs of varous age groups, showing essentially no difference, and are compared with the respective data for human erythrocytes. Thrombocytes and leukocytes were removed and separation of the red cells into reticulocytes and normocytes was done. This proved a higher level of phospholipid phosphate metabolic activity in neonatal pig erythrocytes--comparable to that in human red blood cells--according to their well known higher glucose permeability and glycolytic rate. Further evidence is accumulated by the results presented that the metabolic differences in neonatal pig red cells are due to a distinct neonatal erythrocyte population and not to the increased number of immature cells present in the circulation.
D000818:Animals; D000831:Animals, Newborn; D004912:Erythrocytes / Q000378:metabolism*; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000378:metabolism*; D008566:Membranes; D010743:Phospholipids / Q000378:metabolism*; D013552:Swine
D008563:Membrane Lipids; D010743:Phospholipids
In vitro-Einbau von (33P)-Orthophosphat in die Phospholipide der Erythrozytenmembran neugeborener und adulter Hausschweine