[The effect of uricosuric and uricostatic drugs on DNA metabolism].
Some of the drugs, which are used in therapy of gout were tested with respect to their influence on semi-conservative DNA-synthesis and DNA-repair in vitro, using different cell systems. The agents (Benzbromarone, Allopurinol, Thiopurinol) were investigated in concentrations similar to blood-levels in normal therapy. The cells were irradiated with ultraviolet or ionizing radiation in order to damage their DNA and DNA-repair (unscheduled DNA-synthesis) was estimated by measuring the incorporation of radioactively labeled thymidine into acid insoluble material under appropriate conditions. The substances showed different effects on DNA-synthesis as well as on DNA-repair, even then, when their supposed influence on enzymes of nucleotide metabolism, was similar.
D000493:Allopurinol / Q000031:analogs & derivatives / Q000494:pharmacology; D001553:Benzbromarone / Q000494:pharmacology; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000378:metabolism* / Q000528:radiation effects; D006074:Gout Suppressants / Q000494:pharmacology*; D010117:Oxypurinol / Q000494:pharmacology; D011839:Radiation, Ionizing; D013936:Thymidine / Q000378:metabolism; D014466:Ultraviolet Rays; D014528:Uricosuric Agents / Q000494:pharmacology*
D006074:Gout Suppressants; D014528:Uricosuric Agents; D001553:Benzbromarone; D000493:Allopurinol; C005832:tisopurine; D004247:DNA; D010117:Oxypurinol; D013936:Thymidine
Untersuchungen zur Wirkung von urikosurisch und urikostatisch wirksamen Substanzen auf den DNA-Stoffwechsel