Determination of plasma amino acids in small samples with the use of Dansyl-chloride.
A radiochemical method based on dansylation of plasma samples with Dansyl-chloride and thin alyer chromatography on polyamide sheets is presented for the quantitative individual determination of plasma amino acids in very small blood samples. The results are first corrected with a norvaline internal standard and secondly with specific recovery factors for each amino acid. The results agree slosely with other methods and with already published plasma normal amino acids values in healthy adult rats.
D000596:Amino Acids / Q000097:blood*; D000818:Animals; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer / Q000379:methods; D003619:Dansyl Compounds; D008832:Microchemistry; D051381:Rats
D000596:Amino Acids; D002250:Carbon Radioisotopes; D003619:Dansyl Compounds