Properties of phage-receptor lipopolysaccharide from Pseudomonas morsprunorum.
Lipopolysaccharide (LPS) of Pseudomonas morsprunorum was extracted with hot phenol and purified by repeated centrifuging followed by either block electrophoresis or gel filtration. LPS from a virulent isolate exhibited specific phage inactivation (PI50 = 0.05 mug LPS ml-1), whereas LPS from an avirulent phage-resistant mutant did not. LPS was considered pure when a single band was detected following sodium dodecyl sulphate-cellulose acetate electrophoresis (pH 7.4). It was not phytotoxic when inoculated into cherry leaves at concentrations up to I mg ml-1, but produced weak chlorosis in bean and tobacco at 2 mg ml-1: no visible symptoms appeared after treatment with lower concentrations. The chemical composition of the LPS was partly determined.
D001435:Bacteriophages; D008070:Lipopolysaccharides / Q000032:analysis / Q000494:pharmacology / Q000502:physiology*; D010944:Plants / Q000187:drug effects; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000494:pharmacology / Q000502:physiology*; D011549:Pseudomonas / Q000032:analysis*
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial