UDP-N-acetyl-D-glucosamine-asparagine sequon N-acetyl-beta-D-glucosaminyl-transferase-activity in human serum.
Serum contains a sugar transferase which is able to catalyse the glycosylation in vitro of the asparagine residue present in the sequence Asn.Leu.Thr in bovine pancreatic ribonuclease. UDP-2-Acetamido-2-deoxy-D-glucose (UDP-N-acetyl-D-glucosamine) acts as a donor, although the mechanism of the transfer is unexplored. Spermidine and Mn2+, as well as CDP-choline, can act as activators for the reaction. Monoglycosylated ribonuclease (ribonuclease-GlcNAc) has been separated (23% yield) from unreacted ribonuclease A by affinity chromatography on a column of wheat-germ agglutinin bound to Sepharose, and characterised. A possible reason for the presence of the enzyme in serum is suggested.
D000117:Acetylglucosamine; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001216:Asparagine; D002417:Cattle; D002846:Chromatography, Affinity; D003566:Cytidine Diphosphate Choline / Q000494:pharmacology; D006602:Hexosyltransferases / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D010179:Pancreas / Q000201:enzymology; D011073:Polyamines / Q000494:pharmacology; D011485:Protein Binding; D012260:Ribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D014537:Uridine Diphosphate N-Acetylglucosamine
D000596:Amino Acids; D011073:Polyamines; D008345:Manganese; D014537:Uridine Diphosphate N-Acetylglucosamine; D003566:Cytidine Diphosphate Choline; D001216:Asparagine; D006602:Hexosyltransferases; D012260:Ribonucleases; D000117:Acetylglucosamine