title: Plasma pentobarbitone levels. Estimation by gas-liquid chromatography after clinical doses.
abstract: A simple and relatively quick method of estimating pentobarbitone in plasma samples is reported using an ether extraction and gas-liquid chromatography. Glutethimide was used as the internal standard and proved reliable. Reproducible results were obtained in plasma following 100 mg pentobarbitone given by mouth or intramuscular injection. Evaporation in a heating block at 37 degrees C and storage of samples at -20 degrees C prior to analysis is recommended.
mesh_terms: D000328:Adult; D001793:Blood Preservation; D002849:Chromatography, Gas / Q000379:methods; D004986:Ether; D005984:Glutethimide; D006801:Humans; D008722:Methods; D010424:Pentobarbital / Q000097:blood* / Q000506:poisoning
chemical_list: D004986:Ether; D005984:Glutethimide; D010424:Pentobarbital