title: Experimental amniocentesis and teratogenesis. 1. Evaluation of the intra-amniotic route of treatment in teratological studies.
abstract: Experiments were designed to evaluate the usefulness of the intra-amniotic route of administration of test-substances in teratological studies. Particular attention was given to the differences in fetal parameters of growth and development and the incidence of fetal mortality between treated and untreated offspring which might be induced as a result of the treatment. Amniocentesis on gestational days 14, 15 or 16 produced a high incidence of fetal mortality and a significant reduction in the weights and crown-rump lengths of treated offspring. With increasing gestational age at the time of treatment and by reducing the volume of solvent injected, fetal mortality was decreased.
mesh_terms: D000650:Amnion; D000818:Animals; D005260:Female; D005313:Fetal Death / Q000139:chemically induced; D005333:Fetus / Q000502:physiology; D005865:Gestational Age; D006128:Growth; D007267:Injections; D011247:Pregnancy; D051381:Rats; D013723:Teratogens / Q000008:administration & dosage*
chemical_list: D013723:Teratogens