title: Intestinal absorption of vitamin B12 in patients with chronic pancreatic insufficiency and the effect of human duodenal juice on the intestinal uptake of vitamin B12.
abstract: The mean absorption of vitamin B12 (Schilling test) was 13.1 +/- 1.0 (% +/- S.E.M.) in 21 patients with chronic pancreatic insufficiency and 17.6 +/- 1.4 in 13 control patients (p less than 0.01). There was no correlation between pancreatic bicarbonate production after secretion stimulation and vitamin B12 absorption in the patient group (r = 0.117). Human duodenal juice reduced the uptake of 57CoB12-rat intrinsic factor (IF) by perfused rat small intestinal segments in vivo (p less than 0.01) as well as the uptake of 57CoB12-human IF by purified guinea-pig intestinal brush borders in vitro (p less than 0.01). The results confirm reduced absorption of vitamin B12 in chronic pancreatic insufficiency, but the mechanism remains uncertain.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002908:Chronic Disease; D004386:Duodenum; D005260:Female; D006168:Guinea Pigs; D006358:Hot Temperature; D006801:Humans; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D007437:Intrinsic Factor / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010182:Pancreatic Diseases / Q000378:metabolism*; D010189:Pancreatic Juice / Q000378:metabolism; D051381:Rats; D012546:Schilling Test; D014805:Vitamin B 12 / Q000378:metabolism*
chemical_list: D001639:Bicarbonates; D007437:Intrinsic Factor; D014805:Vitamin B 12