Biochemical and cytochemical studies on adenylate cyclase activity in the developing rat submandibular gland: differentiation of of the acinar secretory compartment.
To investigate membrane changes in development of the exocrine cells of the rat submandibular gland (SMG), biochemical and cytochemical studies of adenylate cyclase activity were performed on prenatal and postnatal glands. SMG rudiments and glands were studied from 15 days of gestation op to birth and 1, 2, 3, 4 and 24 weeks after birth. Glands were chemically assayed for adenylate cyclase activity using the procedures of Salomon and coworkers and cytochemically studied using a procedure which was verified biochemically. At 15-16 days of gestation basal adenylate cyclase activity was low and no staining could be observed. Adenylate cyclase activity rose six-fold from the 16th to the 18th day of gestation. Adenylate cyclase staining became evident along the surface of most of the cells of the rudiment at this time. Basal adenylate cyclase activity remained relatively constant from the 18th day of gestation up to 24 weeks of age. However, sequential changes were seen in the cytochemical localization, especially in relation to the apical plasma membrane of the developing secretory cells.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D002454:Cell Differentiation; D002462:Cell Membrane / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005865:Gestational Age; D006651:Histocytochemistry; D008854:Microscopy, Electron; D051381:Rats; D013363:Submandibular Gland / Q000196:embryology / Q000254:growth & development* / Q000648:ultrastructure
D000262:Adenylyl Cyclases