Hyaluronic acid produced by human synovial fibroblasts. Effect of polyinosinic-polycytidylic acid (poly I:C) and interferon.
Poly I:C (polyinosinic-polycytidylic acid) stimulated hyaluronic acid production by rheumatoid and non-rheumatoid human synovial fibroblasts. Stimulation was dose dependent and was inhibited by acetylsalicyclic acid and indomethacin. Poly I and Poly C, when separately added, had no stimulatory effect on hyaluronic acid production, and Poly A:U had only a slight effect on this parameter. Cells grown with Poly I:C were virus resistant and interferon was detected in their medium. Human interferon had also a dose-dependent stimulatory effect on hyaluronic acid production by synovial cells. A possible interferon-mediated relationship between virus infection and pathologic accumulation of joint fluid is suggested.
D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D007372:Interferons / Q000494:pharmacology*; D011063:Poly A-U / Q000494:pharmacology; D011066:Poly C / Q000494:pharmacology; D011069:Poly I / Q000494:pharmacology; D011070:Poly I-C / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013582:Synovial Fluid / Q000166:cytology*
D011063:Poly A-U; D011069:Poly I; D011066:Poly C; D006820:Hyaluronic Acid; D007372:Interferons; D011070:Poly I-C