title: The evolution of early fibromuscular lesions hemodynamically induced in the dog renal artery. I. Light and transmission electron microscopy.
abstract: In view of the important roles of arterial intimal fibromuscular lesions as precursors of atherosclerotic plaque and occlusive lesions in arterial reconstructions, a model has been developed for the rapid hemodynamic induction of these lesions by anastomosis of the dog right renal artery to the inferior vena cava. Light and transmission electron microscopic observations were made on the arterial shunt after periods of rapid flow ranging form 10 minutes to 2 hours to identify initial factor(s) and evolutionary mechanisms in the etiology of the lesions. The sequence of events included aberrations in ruthenium red staining of the endothelial luminal membrane at 10 minutes, multilayered thickening of the subendothelial basement membrane (BM) at 15 minutes, and initial reorientation and migration of smooth muscle cells (SMC) into the intima along with the appearance of areas of degeneration of the internal elastic lamina (IEL) at 30 minutes. The endothelial cells were still intact in some areas overlying the SMC migration and IEL degeneration, but they were separating from the surface in other such areas. As subendothelium became exposed, some platelet adherence was noted. By 2 hours, the entire wall reaction was fully developed. Initial observations indicate that in the evolution of this hemodynamically induced lesion visible alteration in the endothelial cells is not prerequisite to degeneration of the underlying IEL and reorientation and migration of medial SMC.
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000209:etiology* / Q000473:pathology; D004195:Disease Models, Animal; D004285:Dogs; D004727:Endothelium / Q000473:pathology; D006439:Hemodynamics; D008297:Male; D009130:Muscle, Smooth / Q000473:pathology; D012077:Renal Artery / Q000473:pathology* / Q000637:transplantation / Q000648:ultrastructure; D012078:Renal Artery Obstruction / Q000209:etiology* / Q000473:pathology; D012430:Ruthenium Red
chemical_list: D012430:Ruthenium Red