Removal of a bound ligand from a macromolecule by gel filtration.
If a solution of macromolecule of concentration c and volume a, initially saturated with a ligand of dissociation constant K, is gel filtered through a volume v of solution initially free of ligand, the saturation y with which it emerges is given by the equation y-lny-1=Kv/ca.
D001665:Binding Sites; D002850:Chromatography, Gel; D008024:Ligands / Q000302:isolation & purification*; D046911:Macromolecular Substances
D008024:Ligands; D046911:Macromolecular Substances