Effect of carbon monoxide exposures on erythrocytic 2,3-DPG in rabbits.
To determine if an exposure to lower levels of carbon monoxide (CO) produces quantitative changes in erythrocytic 2,3-diphosphoglycerate (2,3-DPG), eight rabbits were exposed to 100 ppm CO for 5 h resulting in approximately 20% carboxyhemoglobin (HbCO). 2,3-DPG was determined before exposure and immediately after exposure as well as every 3 h during the 24 h following exposure. To determine if intermittent CO exposures over a prolonged period of time affect 2,3-DPG, an additional 12 rabbits were exposed to 250 ppm CO for three intermittent periods totaling 300 min daily for 14 days (30% HbCO). 2,3-DPG was determined once per animal at the end of each day's exposure period. All animals served as their own controls in both experiments. Neither the results of the 24-h time course nor those of the 14-day time course showed significant differences in mean 2,3-DPG between controls and exposed animals at any sampling time. Apparently erythrocytic 2,3-DPG plays neither a compensating nor aggravating role in respect to the hypoxia induced by these levels of HbCO in the rabbit.
D000136:Acid-Base Equilibrium; D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology*; D002263:Carboxyhemoglobin / Q000502:physiology; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004781:Environmental Exposure; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D000860:Hypoxia / Q000503:physiopathology; D008297:Male; D011817:Rabbits
D004163:Diphosphoglyceric Acids; D002248:Carbon Monoxide; D002263:Carboxyhemoglobin