Purification of tRNATrp, tRNAVal, and partial purification of tRNAIle and tRNAMfet from beef liver.
tRNATrp from beef lever has been purified by classical chromatographical methods. Total tRNA, prepared on a large scale (total aminoacid acceptance 1280 pmol/A260 unit) was submitted to chromatography on benzoylated-DEAE cellulose, then DEAE Sephadex. The major species accepting tryptophan issued from the second chromatography was aminoacylated with [14C] tryptophan and chromatographed on benzoylated-DEAE cellulose. The tRNA carrying the radioactive label was eluted in the ethanolic region. After stripping, the resulting tRNATrp has an acceptance of 1800 pmol/A260 unit. No isoacceptors could be demonstrated by chromatography of the pure species on RCP 5 in 6 M urea. The yield in pure tRNATrp was currently in the range of 25 to 30 percent of the total tryptophan acceptance of the starting curde tRNA.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002417:Cattle; D007532:Isoleucine; D008099:Liver / Q000378:metabolism*; D008715:Methionine; D012343:RNA, Transfer / Q000302:isolation & purification* / Q000378:metabolism; D014364:Tryptophan; D014633:Valine
D000596:Amino Acids; D007532:Isoleucine; D014364:Tryptophan; D012343:RNA, Transfer; D008715:Methionine; D014633:Valine