title: Heat-sensitive DNA-binding activity of the cI product of bacteriophage lambda.
abstract: The binding of lambda gene cI product to lambda DNA was studied at temperatures from 0 degrees C to 46 degrees C. Binding activity of the products of cIts mutants was higher at 22 degrees C than at 0 degrees C, 26 degrees C or 30 degrees C. Both cI+ and cIts products lost DNA-binding activity at 46 degrees C, but after subsequent cooling to 22 degrees C, they regained 50-100% of their activity.
mesh_terms: D003080:Cold Temperature; D003090:Coliphages / Q000378:metabolism*; D004267:DNA Viruses; D004279:DNA, Viral / Q000378:metabolism*; D006358:Hot Temperature; D009154:Mutation; D011485:Protein Binding; D014176:Protein Biosynthesis; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins