The use of sodium borate impregnated silica gel plates for the separation of 3-0-methyl catecholamines from their corresponding catecholamines.
The use of sodium borate impregnated silica gel plates for the chromatographic separation of the catecholamines noradrenaline, adrenaline, and isoprenaline from their respective 3-0-methylated derivatives, normetanephrine, metanephrine, and methoxy-isoprenaline, is described. The parent catecholamines remain at the origin of the plates while the 3-0-methylated derivatives concentrate in discrete bands at the upper edge of the borate impregnated area (the "borate front").
D002395:Catecholamines / Q000032:analysis*; D002850:Chromatography, Gel; D004837:Epinephrine / Q000032:analysis; D007545:Isoproterenol / Q000031:analogs & derivatives / Q000032:analysis; D008676:Metanephrine / Q000032:analysis; D008722:Methods; D009638:Norepinephrine / Q000032:analysis; D009647:Normetanephrine / Q000032:analysis
D002395:Catecholamines; D009647:Normetanephrine; D008676:Metanephrine; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine