[Chicken erythrocyte nuclei: composition and analysis of phospholipids].
While many works analyse lipid composition of cellular nuclei, essentially of liver, few describe bird erythrocyte nuclei. For them very discordant results are reported due to very different methods of preparation. In the method we adopted a good reproducibility is controlled by electron microscopy, evaluation of specific enzymatic activities and by protein, RNA and DNA determinations. Total lipid content and phospholipid composition were determined showing, in agreement with other works, the predominance of phosphatidylcholine and phosphatidylethanolamine. On the contrary, the nuclear fraction is poor in sphingomyelin, which is essentially a plasma membrane phospholipid.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis* / Q000648:ultrastructure; D002645:Chickens; D004912:Erythrocytes / Q000032:analysis* / Q000648:ultrastructure; D006651:Histocytochemistry; D008055:Lipids / Q000097:blood; D008854:Microscopy, Electron; D010743:Phospholipids / Q000097:blood*
D008055:Lipids; D010743:Phospholipids
Les noyaux de globules rouges de poussin: composition et analyse des phospholipides