Zonation of the adrenal cortex. II. Effect of BSA on coupling efficiency of mitochondria isolated from the zona glomerulosa of the bovine adrenal cortex.
Effect of bovine serum albumin on coupling efficiency of mitochondria isolated from the zona glomerulosa of the bovine adrenal cortex in various media was examined polarographically and electron microscopically. Albumin restored the coupling efficiency of mitochondria isolated from the zona glomerulosa regardless of isolation media when succinate or malate was oxidizable substrate. Respiratory controls greater than 5 were obtained. Albumin, however, had no effect when glutamate, beta-hydroxybutylate and pyruvate were the oxidizable substrates. The conditions have been found under which mitochondria of the zona glomerulosa stay in the orthodox configuration and yet coupled.
D000302:Adrenal Cortex / Q000187:drug effects / Q000648:ultrastructure*; D000311:Adrenal Glands / Q000648:ultrastructure*; D000818:Animals; D002417:Cattle; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects* / Q000648:ultrastructure; D010101:Oxygen Consumption; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013696:Temperature
D012710:Serum Albumin, Bovine