title: Rat leukaemia cell line grown on a chemically defined medium.
abstract: Leukaemia cells of C12/0 line, derived from Gross virus-induced W/Fu rat thymoma, have been successfully maintained in a completely synthetic, serum-free RPMI 1640 medium. The cells propagated as good as in serum-supplemented medium, though they could not grow from a low cell density. Cells adhered to the substratum and did not survive. This problem was overcome by use of silicone-coated substratum, or of microexudate from C12/0 cells adsorbed on the substratum. The cells underwent degeneration soon after they reached their maximum density. C12/0 cells released macromolecules might possibly have both growth-promoting and toxic effect on the cell itself.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D003470:Culture Media; D006133:Growth Substances / Q000378:metabolism; D007942:Leukemia, Experimental / Q000378:metabolism / Q000473:pathology*; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D051381:Rats
chemical_list: D000596:Amino Acids; D003470:Culture Media; D006133:Growth Substances