Studies on the membrane glycoprotein defect of En(a-) erythrocytes. II. MN antigenic properties of En(a-) erythrocytes.
En(a-) and EnaEn red cells from different sources were studied using biochemical and serological methods. The results suggest that Finnish En(a-) erythrocytes contain only "N" but no M antigenic properties. Data on the members of the English En(a-) family suggest that English En(a-) red cells exhibit a combination of three rare alterations. The English En(a-) individuals are apparently heterozygous for the defects En and Ms, to all appearances, converted to a M ("M") antigen. These extraordinary data are brought to light by investigations on the children of the propositus (G.P. and J.P.), whose red cells are M-N+S-s+"N"+"M"- and M-N+S+s+"N"+"M"+ respectively. Sodium dodecylsulphate polyacrylamide gel electrophoretic results indicate that the MN glycoprotein content is decreased by about 50% in all English S+s+EnaEn red cells, the Ss glycoprotein being normal. G.P. erythrocytes, however, have only about half of the normal MN and Ss glycoprotein content.
D001789:Blood Group Antigens; D002918:Chymotrypsin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004739:England; D004910:Erythrocyte Membrane / Q000276:immunology; D005387:Finland; D006023:Glycoproteins; D006386:Hemagglutination Tests; D006801:Humans; D008951:MNSs Blood-Group System; D008565:Membrane Proteins; D014357:Trypsin / Q000494:pharmacology
D001789:Blood Group Antigens; D006023:Glycoproteins; D008951:MNSs Blood-Group System; D008565:Membrane Proteins; D002918:Chymotrypsin; D014357:Trypsin