Sensitivity of swine buffy coat culture to infection with hog cholera virus.
The susceptibility of swine buffy coat (BC) cultures inoculated with hog cholera (HC) virus in the presence of homologous antiserum was greater than that of a pig kidney (PK-15) cell line similarly inoculated. The virus was isolated from BC cultures grown in the presence of 0.1% hyperimmune serum, whereas it could not be consistently recovered from the PK-15 cell line in which hyperimmune serum concentrations exceeded 0.025%. Maximal viral titers in BC culture were reached between postinoculation days 4 and 8. Peak titers were not influenced by the age of cells at infection or dose of virus. Data were present in support of the hypothesis that the decrease of HC viral isolations in BC cultures from HC epizootics was related to lessened use of attenuated vaccines.
D000818:Animals; D002478:Cells, Cultured; D006691:Classical Swine Fever / Q000382:microbiology*; D012328:RNA Viruses / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D013552:Swine