[Analysis of influenza virus reproduction under conditions of chronic infection].
The results of comparative studies of influenza virus, original and produced in KM cells under conditions of chronic infection (KM WSN) are presented. The WSN KM virus was shown to band in the density range of 1.18 to 1.23 g/ml, with maximum at a.215 g/ml. The electrophoretic analysis of the standard virus RNA revealed mostly heavy fragments. The analysis of a total RNA preparation from the virus population produced by chronically infected cells revealed both heavy and light RNA fragments. The total synthesis of cellular DNA and RNA was slightly inhibited by KM WSN. A mechanism of persistence is suggested associated with continuous production of defective non-infectious particles.
D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000096:biosynthesis; D003673:Defective Viruses; D009980:Influenza A virus / Q000302:isolation & purification; D012367:RNA, Viral / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication
D004279:DNA, Viral; D012367:RNA, Viral; D013936:Thymidine; D014529:Uridine
Analiz reproduktsii virusa grippa v usloviiakh khronicheskoĭ infektsii