Determination of aflatoxins in groundnut meals by high performance liquid chromatography.
A method for determination of aflatoxins in groundnut meals by high performance liquid chromatography (HPLC) was investigated. After extraction and purification according to the AOAC "CB" method, an extract is injected on a Corasil II column and eluted by n-hexane/chloroform/ethanol (50+49.7+0.3), Detection is performed with an UV detector. The developed method makes it possible to determine aflatoxins B1, B2, G1, and G2. The detection limit for aflatoxin B1 is 50 p.p.b. using a 254-nm UV detector, or less with more effective detectors (absorption at 350-360 nm).
D000348:Aflatoxins / Q000302:isolation & purification*; D010367:Arachis / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D007194:India
D000348:Aflatoxins