[Diagnostics of lactose-malabsorption: value of tolerance tests and 14CO2 exhalation test in patients with and without lactase deficiency (author's transl)].
Lactose-tolerance-test (LTT), ethanol-lactose-tolerance-test (ELTT), 14CO2 breath test and 14C-glucose determination were simultaneously performed in 27 healthy subjects, 16 patients with a Billroth II gastrectomy and 6 patients with a malabsorption syndrome. Intestinal mucosal lactase was absent or significant diminished in 5 of the B II cases and in all patients with malabsorption. In the lactase deficient patients a diminished serum glucose rise after ingestion of 50 g lactose was observed in LTT as well as in ELTT. False positive results in LTT could not be prevented by performing the ELTT. Furthermore the ELTT is not suitable for ambulant investigations because of the required high ethanol load of 0.5 g/kg. Most reliable results were obtained by determination of 14C-serum-glucose after oral application of about 15 muCi of 14C lactose. In respect to lactase level neither false positive nor false negative results were observed. For clinical investigations the procedure of isolation and measurement of 14C-glucose is too laborious however. 14CO2-exhalation test cannot be recommended because of many false positive and false negative results. Moreover 14CO2-exhalation seemed to be insensible and predominant depending on factors other than lactose absorption.
D001786:Blood Glucose / Q000032:analysis / Q000378:metabolism; D002245:Carbon Dioxide / Q000032:analysis; D002250:Carbon Radioisotopes; D000431:Ethanol; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D005693:Galactosemias; D005696:Galactosidases / Q000032:analysis / Q000097:blood; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology; D007785:Lactose; D007787:Lactose Intolerance / Q000175:diagnosis*; D007789:Lactose Tolerance Test / Q000379:methods*; D011178:Postgastrectomy Syndromes; D012119:Respiration
D001786:Blood Glucose; D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D000431:Ethanol; D005696:Galactosidases; D007785:Lactose
Diagnostik der Laktose-Malabsorption. Untersuchungen zur Wertigkeit von Toleranztests und 14CO2-Exhalationsmessung bei Patienten mit und ohne Laktasemangel