Mechanism of morphine block of electrical activity in ganglia of Auerbach's plexus.
A study was made of the effect of morphine on electrical activity within single ganglia of Auerbach's plexus of guinea pig longitudinal muscle-myenteric plexus as monitored by means of external electrodes. Morphine produces a concentration dependent block of single spike activity. This effect is competitively antagonized by naloxone. The ED50 for morphine effect is about 7 X 10(-7) M. Naloxone and dextrorphan have no effect on electrical activity. Acetylcholine in the concentration range of 10(-7)--10(-5) M augments electrical activity of ganglia. Morphine has little if any effect on the enhanced stimulation produced by acetylcholine thus indicating that the drug does not act directly upon the ganglion. Our results suggest that a specific opiate receptor is present on the preganglionic nerve terminals and that morphine and other opiates block ganglionic transmission by inhibiting the release of preganglionic acetylcholine.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D003864:Depression, Chemical; D003917:Dextrorphan / Q000494:pharmacology; D004558:Electric Stimulation; D004594:Electrophysiology; D005724:Ganglia / Q000502:physiology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D009020:Morphine / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects; D009197:Myenteric Plexus / Q000502:physiology*; D009270:Naloxone / Q000494:pharmacology
D003917:Dextrorphan; D009270:Naloxone; D009020:Morphine; D000109:Acetylcholine