Fertility of bull semen frozen with beta-amylase, beta-glucuronidase, and catalase.
"Capacitase," a product combining beta-amylase and beta-glucuronidase, was compatible with survival of bull spermatozoa frozen in whole milk-glycerol extender at final concentrations per ml of 0, 5, 10, and 20 mug of beta-amylase combined with 0, 75, 150, and 300 units of beta-glucuronidase, respectively. Bull semen was frozen in whole milk-glycerol extender containing the three lower concentrations of enzymes tested in the previous trial and used to inseminate 9057 first-service cows within 4 mo of freezing. The 60- to 90-day percent nonreturns were 74.6, 75.6, and 75.0. The same treatments plus a fourth one containing 10 mug of catalase per ml were fertility tested in another trial. Insemination of 16,842 cows resulted in 75.6, 74.1, 74.6, and 74.2% nonreturns. In this trial semen was held immersed in liquid nitrogen and distributed for immediate use each mo for 6 mo. There was no change in fertility during 6 mo of continuous storage at --196 C. Under the conditions tested neither catalase nor beta-amylase with beta-glucuronidase enhanced fertility of frozen bull semen.
D000681:Amylases / Q000494:pharmacology; D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002417:Cattle; D004357:Drug Synergism; D005260:Female; D005298:Fertility / Q000187:drug effects; D005615:Freezing; D005966:Glucuronidase / Q000494:pharmacology; D007315:Insemination, Artificial / Q000662:veterinary*; D008297:Male; D013094:Spermatozoa / Q000502:physiology; D014021:Tissue Preservation
D002374:Catalase; D000681:Amylases; D005966:Glucuronidase