[Effect of silymarin on the total dry mass of hepatocytes inacute poisoning by phalloidin and alpha-amanitine (author's transl)].
The effects of silymarin on the total dry mass and class pattern of rat hepatocytes have been studied during acute poisoning by phalloidin and alpha-amanitine. Phalloidin (2/5 of the LD50) after 3 h causes a marked change in the hepatocyte class pattern due to a displacement of a high percentage of cells in the intervals among classes, while the cell dry mass increases slightly. alpha-Amanitine (1/4 or 1/2 of the LD50) after 3 h causes a decrease in the number of classes of hepatocytes due to a disappearance of the heavier ones, a displacement of cells in the intervals among classes, an appearance of very light cells, and a decrease by about 25% in the mean dry mass of the hepatocytes. Silymarin, administered 30 min before poisoning, prevents all the changes due to 2/5 of the LD50 of phalloidin and to 1/4 of the LD50 of alpha-amanitine, and strongly reduces the effects of 1/2 of the LD50 of alpha-amanitine. The effects of alpha-amanitine and phalloidin and the protective action of silymarin on the dry mass and class pattern of hepatocytes are discussed.
D000546:Amanitins / Q000506:poisoning*; D000818:Animals; D001699:Biometry / Q000379:methods; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000473:pathology; D005419:Flavonoids / Q000627:therapeutic use*; D008099:Liver / Q000473:pathology; D008297:Male; D008857:Microscopy, Interference; D009842:Oligopeptides / Q000506:poisoning*; D010590:Phalloidine / Q000506:poisoning*; D051381:Rats; D012838:Silymarin / Q000627:therapeutic use*
D000546:Amanitins; D005419:Flavonoids; D009842:Oligopeptides; D012838:Silymarin; D010590:Phalloidine
Wirkung von Silymarin auf den Gesamtfeststoffgehalt der Leberzellen bei akuter Vergiftung mit Phalloidin und alpha-Amanitin