title: Active potassium transport in reticulocytes of high-K+ and low-K+ sheep.
abstract: The kinetics of active K+ transport were studied in immature red blood cells cells from high-K+ and low-K+ sheep particulary with respect to the effects of varying intracellular K+ concentration, [K]i. Comparison was made with active transport, or pump, activity in mature high-K+ and low-K+ red cells. Reticulocytes from both types of sheep had much higher maximal active K+ influxes than did mature cells. In both types of reticulocytes, and in mature high-K+ cells as well, the pump was relatively insensitive to increasing [K]i. In contrast, intracellular K+ markedly inhibited the pump in mature low-K+ cells. Active K+ transport in low-K+ reticulocytes, however, as in mature low-K+ cells, is stimulated by specific isoimmune anti-L serum. Therefore the K+ pumps of high-K+ and low-K+ reticulocytes have similar kinetic properties. Maturation of the red cells, involving inactivation of most of the pump activity in both cell types, results in mature high-K+ and low-K+ cells with K+ pumps of very different kinetic characteristics.
mesh_terms: D000740:Anemia / Q000097:blood; D000818:Animals; D001693:Biological Transport, Active; D004920:Erythropoiesis; D007700:Kinetics; D011188:Potassium / Q000097:blood*; D012156:Reticulocytes / Q000378:metabolism*; D012756:Sheep
chemical_list: D011188:Potassium