[Optimization of the culture medium and role of its components in the biosynthesis of exopolysaccharides by Mycobacterium lacticolum].
A medium has been selected for providing the maximum synthesis (7-8 g/litre) of a free exocellular polysaccharide by Mycobacterium lacticolum 121. The following concentrations of the components are optimal for the biosynthesis: hexadecane, 5.0-6.5% (by volume); NaNO3, 2.0-4.0 g/litre; Na2HPO4-12H2O, 1.0-1.5 g/litre; KCl, 1.0-1.5 g/litre. An increase in the concentration of sodium nitrate and phosphate over 4.0 and 1.5 g/litre, respectively, favours growth of the cells but inhibits biosynthesis of the polysaccharide. Concentrations of hexadecane and potassium chloride over 6.5% and 1.5 g/litre, respectively, have no effect of the culture and yield of the polysaccharide.
D000473:Alkanes; D003470:Culture Media; D009161:Mycobacterium / Q000254:growth & development / Q000378:metabolism*; D009566:Nitrates; D010710:Phosphates; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011189:Potassium Chloride
D000473:Alkanes; D003470:Culture Media; D009566:Nitrates; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D011189:Potassium Chloride
Optimizatsiia sredy i znachenie nekotorykh ee komponentov dlia biosinteza ékzopolisakharida Mycobacterium lacticolum