Modification of H-2 antigenic sites by enzymatic treatment influences virus-specific target cell lysis.
Vaccinia virus-infected cells were treated enzymatically to remove H-2 antigenic sites. The effect of this procedure on virus-specific cell-mediated cytolysis (CMC) and virus-specific antibody-mediated cytolysis (AMC) was tested. Due to the inhibition of cellular proteinsynthesis by the vaccinia virus infection, H-2 antigenic sites were not resynthesized while there was a continuous production of viral surface antigens. These cells with a high concentration of viral surface antigens and decreased H-2 determinants could be used as targets in the virus specific AMC which emphasizes the significance of H-2 antigens during recognition of virus-specific determinants by T cells.
D000818:Animals; D000918:Antibody Specificity; D003601:Cytotoxicity Tests, Immunologic; D006649:Histocompatibility Antigens / Q000032:analysis*; D007111:Immunity, Cellular; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D009439:Neuraminidase / Q000494:pharmacology*; D010206:Papain / Q000494:pharmacology*; D011402:Pronase / Q000494:pharmacology*; D014357:Trypsin / Q000494:pharmacology*; D014615:Vaccinia / Q000276:immunology; D014616:Vaccinia virus / Q000276:immunology*
D006649:Histocompatibility Antigens; D009439:Neuraminidase; D014357:Trypsin; D010206:Papain; D011402:Pronase