[Studies on endocrine and spermatogenic testicular function. 5. Relationship between dietary protein quality and blood plasma levels of free and bound testosterone in adult rats].
The blood plasma content of testosterone-linking globulins, free testosterone, and total testosterone in 80 adult Wistar rats which received different qualities of feed proteins was determined by means of balancing dialysis and ultracentrifugation, using 3H-testosterone. The first group received casein with four per cent methionine added, the second maize gluten complement with amino acid, and the third casein with methionine and gelatin in a 2:3 ratio, while the fourth group was fed pure maize gluten diet. (Biological valence declined in the given order.) Assays of 100 ml blood plasma of each animal in the first and second groups gave 0.9 mug of testosterone-linking globulins (0.18 mug in third and fourth groups), about 9.0 mug total testosterone (2.5 mug in third and fourth groups), and some 1.4 mug free testosterone (0.55 mug in third and fourth groups). Discussed in this paper is the possible mechanism of regulation by which feed protein quality leads to variation of free testosterone levels in the blood plasma.
D000818:Animals; D004044:Dietary Proteins / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism* / Q000502:physiology; D013739:Testosterone / Q000097:blood*
D004044:Dietary Proteins; D013739:Testosterone
Untersuchungen zur endokrinen und spermiogenen Hodenfunktion 5. Mitteilung: Zu Beziehungen zwischen Nahrungsproteinqualität und Blutplasmagehalt an freiem und gebundenem Testosteron bei adulten Ratten