Effect of puromycin on the ultrastructure of keratohyalin granules.
Keratohyalin granules (KHG) from control and puromycin-treated hamster cheek pouch epithelium were studied by electron microscopy. The animals were killed 1-4 hours after injection with 75-100 microgram puromycin per gram body weight; controls were injected with saline. The KHG from control animals were round or oval and consisted of a finely granular background matrix containing many small, round, homogeneous electron-dense deposits. The KHG from puromycin-treated animals showed marked variation in morphology and size. They consisted of a coarse granular or speckled matrix and round, club-shaped or comma-shaped electron-dense deposits. Since puromycin is known to inhibit protein synthesis, alterations in KHG structure support previous reports suggesting the protein nature of KHG.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D006224:Cricetinae; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure*; D004848:Epithelium / Q000648:ultrastructure*; D007633:Keratins; D008647:Mesocricetus; D009055:Mouth; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology*
D011691:Puromycin; D007633:Keratins