Acyl-CoA synthetases in guinea-pig liver mitochondria. Purification and characterization of a distinct propionyl-CoA synthetase.
Guinea-pig liver mitochondria contain three soluble ATP-dependent acyl-CoA synthetases: (a) a medium-chain acyl-CoA synthetase, (b) a salicylate activating enzyme, and (c) a propionyl-CoA synthetase. A complete separation of these enzymes has been accomplished and the resulting preparation of propionyl-CoA synthetase (Spec. act. 4 units/mg protein) accepts acetate, propionate and butyrate as substrates with a high preference for propionate.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D001565:Benzoates / Q000378:metabolism; D002087:Butyrates / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000302:isolation & purification / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006168:Guinea Pigs; D007700:Kinetics; D008930:Mitochondria, Liver / Q000201:enzymology*; D011422:Propionates / Q000378:metabolism; D012459:Salicylates / Q000378:metabolism
D000085:Acetates; D001565:Benzoates; D002087:Butyrates; D005227:Fatty Acids; D011422:Propionates; D012459:Salicylates; D003066:Coenzyme A Ligases