Chemical nature of agrocin 84 and its effect on a virulent strain of Agrobacterium tumefaciens.
Agrocin 84, produced by Agrobacterium radiobacter K84, inhibited ribonucleic acid, deoxyribonucleic acid, and protein synthesis and amino acid transport in a susceptible, virulent strain of A. tumefaciens H-38-9. Cell motility was immediately stopped by action of the agrocin, 50% of the cells were killed within 15 min of contact, and the remainder were inhibited. Agrocin 84 is trypsin and pepsin resistant, but chemical analysis indicated a small peptide with a molecular weight of 2,500 containing six different amino acids, including nine molecules of glutamine or glutamic acid and seven molecules of serine.
D000227:Adenine Nucleotides; D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D001430:Bacteriocins / Q000096:biosynthesis / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002850:Chromatography, Gel; D004269:DNA, Bacterial / Q000096:biosynthesis; D008970:Molecular Weight; D009068:Movement / Q000187:drug effects; D012329:RNA, Bacterial / Q000096:biosynthesis; D012231:Rhizobium / Q000187:drug effects* / Q000378:metabolism
D000227:Adenine Nucleotides; D000596:Amino Acids; D001426:Bacterial Proteins; D001430:Bacteriocins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; C013799:agrocin 84