The sulphatase of ox liver. XX. The preparation of sulphatases B1alpha and B1beta.
Sulphatases B1alpha and B1beta (EC 3.1.6.1) have been prepared as apparently homogeneous proteins by chromatography on ConA-Sepharose. Both have a mol. wt. of 56 000, and E1%280nm of 17 and a turnover number of 8600 min-1 with nitrocatechol sulphate as substrate. Their amino acid compositions are identical: like sulphatase A, the sulphatases B are rich in proline and yield glucosamine on hydrolysis. They are not altered by treatment with neuraminidase. Both fractions show strong UDP-N-acetylgalactosamine 4-sulphatase activity, weak iduronate sulphatase activity, but no significant heparan N-sulphatase activity. It is suggested that the physiological activity of sulphatase B is that of the N-acetylgalactosamine 4-sulphatase which is lacking in the Maroteaux-Lamy Syndrome.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001192:Arylsulfatases / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity; D003208:Concanavalin A; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D013045:Species Specificity; D013429:Sulfatases / Q000302:isolation & purification / Q000378:metabolism
D000596:Amino Acids; D007527:Isoenzymes; D003208:Concanavalin A; D013429:Sulfatases; D001192:Arylsulfatases