Identification of a sialoglycopeptide released by self-digestion from human erythrocyte membranes.
Membranes from human O Rhesus-positive erythrocyte 'ghosts' were tested in vitro for their ability to digest their own glycoproteins. 'Ghost' membranes incubated in Tris/HCl buffer, pH 7.4, release a sialoglycopeptide, which contains glucosamine, galactosamine, galactose and mainly polar amino acids. Chemical composition, molecular size and aggregation properties suggest that this glycopeptide may be a fragment of glycophorin.
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D008565:Membrane Proteins; D008970:Molecular Weight; D012795:Sialoglycoproteins / Q000302:isolation & purification / Q000378:metabolism*
D008565:Membrane Proteins; D012795:Sialoglycoproteins