title: Hemoglobin Dakar = Hb Grady: demonstration by a new approach to the analysis of the tryptic core region of the alpha chain and oxygen equilibrium properties.
abstract: Preliminary studies have suggested that in Hb Dakar, histidine alpha112 was substituted by a glutamine. A re-investigation on this hemoglobin is presented in this report. A structural study has been performed using a new approach to analyse the tryptic core region of the human hemoglobin alpha chain. After tryptic digestion of the aminoethylated alpha chain, a secondary digestion of the tryptic core was carried out with chymotrypsin and with another protease, thermolysin. Analyses of the chymotryptic and thermolytic peptides indicated that the structure of Hb Dakar was identical to that of Hb Grady previously described by Huisman et al. who showed the insertion of three amino acid residues in position alpha115 or alpha118. The insertion, which was localized near two residues involved in the alpha1beta1 contact, did not produce a dissociation into dimers. Functional studies demonstrated a a slightly increased oxygen affinity, a lowered cooperativity and a normal Bohr effect. The low amount of the abnormal hemoglobin (8%) may in part be explained by a slight instability of the molecule.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001797:Blood Protein Electrophoresis; D004588:Electrophoresis, Cellulose Acetate; D005914:Globins; D006441:Hemoglobin A; D006455:Hemoglobins, Abnormal; D006801:Humans; D007700:Kinetics; D010100:Oxygen / Q000097:blood*; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D013820:Thermolysin; D014357:Trypsin
chemical_list: D000596:Amino Acids; D006455:Hemoglobins, Abnormal; D010446:Peptide Fragments; D005914:Globins; D006441:Hemoglobin A; D014357:Trypsin; D013820:Thermolysin; D010100:Oxygen