Immunology of DNA. V. Analysis of DNA/anti-DNA complexes.
DNA/anti-DNA complexes were studied by equilibrium density centrifugation in CsC1 and by zonal centrifugation in sucrose gradients. Complexes prepared of circular DNA preparations PM2 DNA or SV40 DNA with anti-DNA obtained from patients with systemic lupus erythematosus were found to be stable under the conditions of CsC1 equilibrium centrifugation. This enabled homogeneous complexes of DNA and anti-DNA of composition AgAb1, AgAb2, AgAb3 etc to be isolated. The stability of these complexes, their banding pattern in CsC1 gradients and their sedimentation behaviour suggest that anti-DNA binds to DNA in a monogamous bivalent way.
D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000032:analysis / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000276:immunology*; D007074:Immunoglobulin G / Q000378:metabolism; D008970:Molecular Weight
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000941:Antigens; D007074:Immunoglobulin G; D004247:DNA