A new approach to assessing collagen turnover by using a micro-assay. A highly efficient and rapid turnover of collagen in rat periodontal tissues.
Measurement of [3H]proline incorporation into newly synthesized and mature collagen in connective tissues was used to compare rates and efficiency of collagen turnover. The approach minimizes label-recycling problems. By using a micro-assay to determine hydroxyproline specific radioactivities, a highly efficient and rapid collagen turnover in rat periodontal tissues was demonstrated.
D000818:Animals; D003094:Collagen / Q000096:biosynthesis* / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D008722:Methods; D010519:Periodontium / Q000378:metabolism*; D011392:Proline / Q000097:blood / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D003094:Collagen; D011392:Proline; D006909:Hydroxyproline