[Protein hydrolysis by immobilized enzymes].
The enzymic hydrolysis of some proteins (insulin-B-chain-S-sulfonate, S-aminoethylated lysozyme, bovine serum albumin) by immobilized peptidolytic enzymes is reported. Sepharose-bound pronase, trypsin and a protease from Thermoactinomyces sp. (MP), the latter both cross linked by glutaric dialdehyde and an exopeptidase mixture containing Sepharose-bound leucine aminopeptidase, carboxypeptidase A and a crude preparation of prolidase were used. After enzymic hydrolysis nearly all amino acids, except proline, were recovered in a 100% yield compared to the value of an acid reference hydrolysate. Tryptophan and methionine, which are partially destroyed by acid hydrolysis in the presence of oxygen could be recovered completely.
D000626:Aminopeptidases / Q000378:metabolism; D002268:Carboxypeptidases / Q000378:metabolism; D006868:Hydrolysis; D007328:Insulin / Q000378:metabolism; D007930:Leucine; D009113:Muramidase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D012685:Sepharose; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014357:Trypsin
D007328:Insulin; D011506:Proteins; D012710:Serum Albumin, Bovine; D012685:Sepharose; D009113:Muramidase; D002268:Carboxypeptidases; D010447:Peptide Hydrolases; D000626:Aminopeptidases; D014357:Trypsin; D007930:Leucine
Untersuchungen zur Hydrolyse von Proteinen durch immobilisierte Enzyme