A procedure for the bulk preparation of brain microsomes with low speed centrifugation.
A method is described for the rapid isolation of microsomes from brains of various species. The method depends on the interaction of calcium with microsomes. The procedure is limited because calcium inhibits certain microsomal enzymes. Magnesium and manganese may be substituted for calcium. However, the substitutions decrease the reliability and efficiency of the procedure.
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D001923:Brain Chemistry; D002118:Calcium; D002413:Cations, Divalent; D002414:Cations, Monovalent; D002458:Cell Fractionation / Q000379:methods; D002498:Centrifugation; D002784:Cholesterol / Q000032:analysis; D003579:Cytochrome Reductases / Q000032:analysis; D008274:Magnesium; D008345:Manganese; D008861:Microsomes / Q000032:analysis / Q000648:ultrastructure*; D009419:Nerve Tissue Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D051381:Rats
D002413:Cations, Divalent; D002414:Cations, Monovalent; D009419:Nerve Tissue Proteins; D008345:Manganese; D012313:RNA; D002784:Cholesterol; D003579:Cytochrome Reductases; D008274:Magnesium; D002118:Calcium