Participation of a trisaccharide-lipid in glycosylation of oviduct membrane glycoproteins.
Preincubation of a hen oviduct membrane preparation with UDP-Nactyl[14C]glucosamine and bacitracin, followed by incubation with GDP-mannose,  leads to formation of a chloroform/methanol (2/1)-extractable glycolipid.  Treatment of the lipid with mild acid results in the release of a trisaccharide shown to have the structure beta-mannosyl-N-acetylglucosamineyl-N-acetylglucosamine.  Incubation of purified trisaccharide-lipid with oviduct membranes in the presence of sodium deoxycholate, Mn2+, and GDP-mannose leads to formation of a labeled glycoprotein with an apparent molecular weight of 25,000...
D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D005260:Female; D006017:Glycolipids / Q000302:isolation & purification / Q000378:metabolism*; D006020:Glycopeptides / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D007700:Kinetics; D008358:Mannose / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D010057:Oviducts / Q000378:metabolism*
D006017:Glycolipids; D006020:Glycopeptides; D006023:Glycoproteins; D008565:Membrane Proteins; D008358:Mannose; D000117:Acetylglucosamine