Inhibition of Echovirus-12 multiplication by N-carbobenzoxy-D-glucosamine.
The glucosamine derivative, N-carbobenzoxy-D-glucosamine (NCBZG) inhibits the multiplication of Echovirus-12 and the synthesis of both virus RNA and protein at a stage in the virus growth cycle after attachment and penetration. However, the compound does not inhibit virus multiplication after the appearance of progeny virus nor after virus RNA has accumulated. Incorporation of radioactive glucosamine and choline into infected and uninfected cultures is inhibited by NCBZG as is the virus-induced increase in choline incorporation. The compound also prevents the appearance of radioactive choline in isolated membranous structures. The compound did not alter significantly the cellular RNA or protein synthesis, plating efficiency of the cells, their growth over a period of several days, nor the virus-directed inhibition of cellular RNA and protein. These findings suggest that the compound inhibits virus multiplication by its effect on the initiation of biosynthesis which appears to require membrane synthesis.
D002460:Cell Line; D002794:Choline / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D029822:Enterovirus B, Human / Q000187:drug effects* / Q000254:growth & development; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis
D012367:RNA, Viral; D014764:Viral Proteins; C013864:N-carbobenzoxy-D-glucosamine; D012313:RNA; D005944:Glucosamine; D002794:Choline