Enzymatic adenylylation by aminoglycoside 4'-adenylytransferase and 2"-adenylyltransferase as a means of determining concentrations of aminoglycoside antibiotics in serum.
A fast and accurate enzymatic estimation of the concentration of aminoglycoside antiobiotics in serum is given by aminoglycoside 4'-adenylyltransferase (synonym: tobramycin adenylyltransferase), a new enzyme recently found in tobramycin-resistant staphylococci (P. Santanam and F. H. Kayser, J. Infect. Dis. [Suppl.], in press). This enzyme is useful in assaying the kanamycins, the butirosins, amikacin, ribostamycin and, if required, the neomycins, tobramycin, and paromomycin. In combination with the assay using aminoglycoside 2''-adenylyltransferase (synonym: gentamicin adenylyltransferase) to determine the level of gentamicin, sisomicin, and tobramycin, a convenient method to estimate a wide range of aminoglycoside antibiotics in patients' sera is described.
D000617:Aminoglycosides / Q000097:blood; D000900:Anti-Bacterial Agents / Q000097:blood*; D004796:Clinical Enzyme Tests; D006801:Humans; D066298:In Vitro Techniques; D008722:Methods; D009713:Nucleotidyltransferases; D013210:Staphylococcus / Q000201:enzymology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D009713:Nucleotidyltransferases; C023169:tobramycin adenylyltransferase