[Utilization of poly U-cellulose and poly U-sepharose for the study of virus-specific RNA of Newcastle disease virus].
Comparative characteristics of poly(U)-cellulose and poly(U)-sepharose used for the study of virus-specific 18S RNA of Newcastle disease virus are presented. Upon chromatography of 18S NDV RNA on any of these columns, approximately 60-70% RNA was adsorbed. The sorbing RNA contained in poly(A) 10-12% of the total amount of adenosine, in contrast to RNA not adsorbed on the column in which poly(A) contained 1-2% of the total amount of adenosine in RNA molecule. The analysis in sucrose density gradient and polyacryl amide gel of the RNAs eluated from the columns showed RNA chromatography under the conditions used not to cause its degradation. The advantages and short-comings of the sorbents used in studies of virus-specific RNA are discussed.
D002482:Cellulose; D002845:Chromatography / Q000379:methods*; D009522:Newcastle disease virus / Q000032:analysis*; D011061:Poly A / Q000032:analysis; D011072:Poly U; D012367:RNA, Viral / Q000032:analysis*; D012685:Sepharose
D012367:RNA, Viral; D011061:Poly A; D011072:Poly U; D002482:Cellulose; D012685:Sepharose
Primenenie poli(U)-tselliulozy i poli(U)-sefarozy dlia izucheniia virusspetsificheskoĭ RNK virusa bolezni N'iukasla