Sensitivity of 15-day mouse fetal liver cells to erythropoietin (ESF). Its practical application to in vitro methods of ESF bioassay.
Demonstration was made of equal reactivity of 13-, 14- and 15-day mouse fetal livers to graded concentrations of Erythropoietin "in vitro". In consequence, replacement by 15-day fetal liver of 13-day liver, currently used as material in Erythropoietin bioassays "in vitro", is proposed as a technical improvement on the method.
D000818:Animals; D001681:Biological Assay / Q000379:methods; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004900:Erythroblasts / Q000166:cytology; D004921:Erythropoietin / Q000032:analysis / Q000494:pharmacology*; D005260:Female; D005333:Fetus / Q000033:anatomy & histology*; D005865:Gestational Age; D006418:Heme / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D051379:Mice; D008809:Mice, Inbred C3H; D011247:Pregnancy
D004921:Erythropoietin; D006418:Heme; D007501:Iron