Determination of free fatty acids as pentafluorobenzyl esters by electron capture gas chromatography.
A method for the determination of free fatty acids in minute serum samples has been developed. The acids are esterified by extractive alkylation, using tetrabutylammonium as a counter ion and pentafluorobenzyl bromide as alkylating reagent. The derivatization of palmitic acid required a reaction time of 25 min. The excess of pentafluorobenzyl bromide is removed by coupling it with a phenoalkylamine and extraction of the product into an acidic aqueous phase. Quantitation is carried out by gas chromatography with electron capture detection. The precision achieved in the determination of 6.2 mug of palmitic acid in 50 mul of mouse serum was 6.1% (S.D.).
D000478:Alkylation; D000818:Animals; D002849:Chromatography, Gas; D004952:Esters / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005464:Fluorobenzenes / Q000032:analysis*; D007202:Indicators and Reagents; D008722:Methods; D051379:Mice; D010169:Palmitic Acids / Q000097:blood
D004952:Esters; D005230:Fatty Acids, Nonesterified; D005464:Fluorobenzenes; D007202:Indicators and Reagents; D010169:Palmitic Acids