Solid phase radioimmunoassay for determination of conjugated cholic acid in serum.
A modified radioimmunoassay for conjugated cholates is presented. In this modification the antibody is chemically bound to Sepharose which enablproducibility (the coefficient of variation between samples is 4%). The whole procedure is carried out at room temperature and with a short incubation time (45 min). Serum can by analysed directly (no extractions or modifications needed). The assay is a suitable tool for liver function testing. A rough indication of total bile acid concentration in serum or bile can also be obtained with this assay.
D002793:Cholic Acids / Q000097:blood*; D003429:Cross Reactions; D006505:Hepatitis / Q000097:blood; D006801:Humans; D007700:Kinetics; D008103:Liver Cirrhosis / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods; D012685:Sepharose; D012710:Serum Albumin, Bovine
D002793:Cholic Acids; D012710:Serum Albumin, Bovine; D012685:Sepharose