Resolution and purification of histones on homologous series of hydrocarbon-coated agaroses.
Hydrophobic chromatography on alkyl-agarose columns has been applied to the fractionation of histones. This paper describes: (a) a two-column method for the resolution of whole histone from calf thymus into its five main components (H1, H2a, H2b, H3 and H4), (b) a rapid one-step procedure for the isolation of the H3 fraction from whole histone, (c) an alternative one-step procedure for the resolution of H3 and H2a (which co-elute during gel exclusion chromatography on Biogel P-60). These experiments are also used for gaining further insight into the mechanism of action of hydrocarbon-coated agaroses.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D006657:Histones / Q000302:isolation & purification*; D006838:Hydrocarbons; D008970:Molecular Weight; D012685:Sepharose; D013329:Structure-Activity Relationship; D013950:Thymus Gland
D006657:Histones; D006838:Hydrocarbons; D012685:Sepharose