A highly efficient method for washing mammalian spermatozoa.
A simple method has been developed for washing spermatozoa: the cell suspension is layered over a solution containing Ficoll and, after centrifugation, the original suspending medium remains as an undiluted layer above the Ficoll solution while the spermatozoa form a loose pellet at the bottom. Removal of the supernatant layers is easily and completely accomplished by aspiration. When ram spermatozoa were washed by this method, as much as 99-6% of the original medium could be removed during a single washing cycle, while up to 98% of the cells were recovered. Mechanical damage to the cells was minimal and repeatability was high.
D000818:Animals; D002417:Cattle; D002470:Cell Survival; D002498:Centrifugation / Q000379:methods; D005362:Ficoll; D006736:Horses; D007444:Inulin; D008297:Male; D012756:Sheep; D013048:Specimen Handling / Q000379:methods*; D013081:Sperm Motility; D013094:Spermatozoa / Q000032:analysis; D013552:Swine
D005362:Ficoll; D007444:Inulin