Cell surface distribution of lectin receptors determined by resonance energy transfer.
The surface topography of concanavalin A (con A) bound to normal and transformed murine fibroblasts has been studied by a new technique involving fluorescence resonance energy transfer (RET), RET can provide a high resolution "map" of the distances separating con A-receptor complexes in single living cells. The distribution of con A is non-random in both normal and transformed cells, but sites are more closely approximated in the transformed. Approximation is induced by the con A but occurs at extremely slow rates indicating that the topography is not primarily determined by simple diffusion of complexes.
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic; D004058:Diffusion; D004735:Energy Transfer; D005347:Fibroblasts; D008856:Microscopy, Fluorescence; D009068:Movement; D011952:Receptors, Concanavalin A / Q000378:metabolism*; D011955:Receptors, Drug / Q000378:metabolism*
D011952:Receptors, Concanavalin A; D011955:Receptors, Drug