Further characterization of a low-molecular weight allergen fragment isolated from the green pea.
A low molecular weight allergen fragment present in the pea dialysate fraction was purified by ion-exchange chromatography and gel filtration. The highly purified allergen fragment inhibits both antigen-indiced passive cutaneous anaphlaxix reactions in guinea-pigs sensitized with rabbit anti-pea extract sera and Prausnitz-Küstner reactions in non-allergic volunteers sensitized with the sera of patients sensitive to green peas. Preliminary analysis of the purified allergen fragment indicates that it is a glycoprotein with a molecular weight of 1800 +/- 250.
D000485:Allergens / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006023:Glycoproteins / Q000032:analysis; D006168:Guinea Pigs; D008970:Molecular Weight; D010323:Passive Cutaneous Anaphylaxis; D010944:Plants / Q000032:analysis*; D012882:Skin Tests
D000485:Allergens; D000596:Amino Acids; D006023:Glycoproteins