Inhibition of murine granulopoiesis in vitro by dexamethasone.
The effect of dexamethasone on mouse bone marrow granulocyte-monocyte precursor cells (CFU-C) was studied in vitro. Dexamethasone inhibited colony formation in the presence of maximally stimulating concentrations of colony-stimulating activity. A mean colony reduction of 55% occurred at 10(-9) M dexamethasone and inhibition was observed at concentrations as low as 10(-12)M. The dexamethasone suppression could be abrogated by progesterone. These studies provide evidence that the committed granulocyte-monocyte stem cell has a glucocorticoid receptor mechanism that when activated results in inhibition of cellular proliferation in vitro.
D000362:Agar; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology; D006098:Granulocytes; D006410:Hematopoiesis / Q000187:drug effects*; D007962:Leukocytes; D008747:Methylcellulose; D051379:Mice; D011239:Prednisolone / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology
D000431:Ethanol; D011374:Progesterone; D003907:Dexamethasone; D000362:Agar; D008747:Methylcellulose; D011239:Prednisolone