Isolation and translation of non-crystallin messenger RNA from calf lens.
Affinith chromatography of lens polyribosomal RNA on oligo(dT)-cellulose yields three fractions. As arule the second fraction has been neglected in other studies reported in the literature. According to our investigations this fraction in particular contains the messengers for the non-crystallin lens proteins.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D007908:Lens, Crystalline / Q000378:metabolism*; D008970:Molecular Weight; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D012335:RNA, Ribosomal / Q000302:isolation & purification / Q000378:metabolism
D012333:RNA, Messenger; D012335:RNA, Ribosomal