title: A general method for isolation of high molecular weight DNA from eukaryotes.
abstract: A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 X 10(6) d and contains very few, if any, single strand breaks.
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000302:isolation & purification*; D004141:Dinoflagellida / Q000032:analysis; D005260:Female; D006801:Humans; D008099:Liver / Q000032:analysis; D008722:Methods; D051379:Mice; D008970:Molecular Weight; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D013950:Thymus Gland / Q000032:analysis; D014461:Ultracentrifugation
chemical_list: D004247:DNA