[Redioimmunoassay for secretin (author's transl)].
The synthesis of a secretin with an elongated N-terminus, namely Nalpha-(deaminotyrosyl-beta-alanyl)- secretin (DATA-secretin), using a conventional strategy, is described. After purification of the product by means of ion exchange chromatography on SP-Sephadex C-25 and by continuous carrier-free electrophoresis, immunological studies on the synthetic DATA-secretin were carried out using secretin antibodies. In comparison to 125iodine-labelled secretin and [Tyr6]secretin, 125iodine-DATA-secretin proved to be by far the best "tracer". Elaboration of a radioimmunoassay for secretin is therefore possible.
D000818:Animals; D000917:Antibody Formation; D008722:Methods; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012633:Secretin / Q000032:analysis* / Q000276:immunology; D012711:Serum Albumin, Radio-Iodinated
D012711:Serum Albumin, Radio-Iodinated; D012633:Secretin
Radioimmunoassay für Sekretin