title: Influence of oxygen tension of the metabolism of vascular smooth muscle: demonstration of a Pasteur effect.
abstract: The influence of variations of oxygen tension on the metabolism of bovine mesenteric arteries was studied in vitro. Glucose uptake, lactate production, glycogen content, adenosine triphosphate (ATP), creatine phosphate (CrP) and incorporation of [14C]leucine into protein were determined. The mesenteric arteries were suspended in Krebs-Henseleit bicarbonate buffer which was aerated with a gas mixture containing 5% CO2,O-95% O2 and N2 to 100%. Reduction of the O2 concentration of the gas phase from 95-20% resulted in little metabolic change. A further reduction from 20-0% O2 increased the lactate production 4-fold, indicating a marked Pasteur effect. At 0% O2 the glucose uptake was moderately increased and the glycogen content was decreased. The tissue level of CrP was reduced at a low oxygen tension and at 0% O2 the ATP content was also lowered. The incorporation of leucine into proteins was reduced at 0% O2.
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002417:Cattle; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism; D066298:In Vitro Techniques; D007773:Lactates / Q000096:biosynthesis; D008638:Mesenteric Arteries / Q000378:metabolism*; D010100:Oxygen; D010313:Partial Pressure; D010725:Phosphocreatine / Q000378:metabolism
chemical_list: D007773:Lactates; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D006003:Glycogen; D005947:Glucose; D010100:Oxygen