Duodenal calcium-binding protein (CaBP) and phosphorus deprivation in growing rats.
The present study has been undertaken to elicit the effects of a low-phosphorous diet on intestinal calcium absorption. Measurements of duodenal calcium-binding protein (CaBP), mucosal calcium, fractional calcium absorption and duodenal calcium transport capacity has been performed in growing rats fed for three weeks a low-phosphorous diet adequate in vitamin-D3. In animals on low-phosphorous diet, the mucosal concentration of CaBP measured by a quantitative competitive binding assay has increased in parallel with the mucosal calcium concentration, the fractional absorption and the calcium transport capacity. Analytical gel electrophoresis of calcium-binding protein in animals fed low-phosphorous diet has shown the presence of a protein band which would involve more than the effect of the stimulated biosynthesis of 1alpha,25-DHCC.
D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis*; D004032:Diet; D004386:Duodenum / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006128:Growth; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D010758:Phosphorus / Q000172:deficiency*; D051381:Rats
D002352:Carrier Proteins; D010758:Phosphorus; D002118:Calcium