Purification of myosin translational control RNA and its interaction with myosin messenger RNA.
Myosin messenger ribonucleoprotein-translational control ribonucleic acid (mRNP-tcNA) from myosin mRNPs found in embryonic chick muscle has been further purified by Dowex chromatography and, from a number of controls, it is suggested that this small RNA is not an artifact produced through the degradation of RNA during its isolation. This highly purified myosin mRNP-tcRNA is shown to have a molecular weight of 10 000 on formamide-acrylamide gels, and reacts stoichometrically (on a 1:1 mole ratio) with myosin mRNA. The stoichiometric interaction between myosin mRNA and myosin mRNP-tcRNP is demonstrated by ists ability to increase the nuclease resistance of the messenger, as well as inhibit its translation in a cell-free amino acid incorporating system.
D000818:Animals; D002474:Cell-Free System; D002642:Chick Embryo; D008970:Molecular Weight; D009218:Myosins / Q000096:biosynthesis; D009693:Nucleic Acid Hybridization; D010442:Peptide Chain Initiation, Translational; D011061:Poly A; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012156:Reticulocytes / Q000378:metabolism; D012260:Ribonucleases; D012261:Ribonucleoproteins
D012333:RNA, Messenger; D012261:Ribonucleoproteins; D011061:Poly A; D012313:RNA; D012260:Ribonucleases; D009218:Myosins