Leukocyte migration inhibition assay (LIF) in nickel contact dermatitis.
A direct assay using the mixed leukocyte migration inhibition technique for leukocyte inhibition factor (LIF) production has shown that nickel-sensitive subjects can be distinguished from nonallergic controls. The difference between the two groups' migrating indexes was obtained with nickel sulfate in 199 medium at a concentration of 150 mug/ml and without prior coupling to a carrier protein. A concise review of the principal in vitro assays for cell-mediated immunity is included.
D002464:Cell Migration Inhibition; D003877:Dermatitis, Contact / Q000175:diagnosis / Q000209:etiology*; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D009532:Nickel / Q000009:adverse effects*
D009532:Nickel