Deuterium isotope effect in the microsomal metabolism of dimethylnitrosamine.
The rates of N-demethylation of dimethylnitrosamine and dimethylnitrosamine-d6 by rat liver microsomes were studied by monitoring formaldehyde production. Deuterium isotope effects of 1.6 and 3.8 were found for the Michaelis constant and the maximum velocity, respectively.
D000818:Animals; D001667:Binding, Competitive; D002273:Carcinogens / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D003903:Deuterium / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000378:metabolism*; D005557:Formaldehyde / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009602:Nitrosamines / Q000378:metabolism*; D051381:Rats
D002273:Carcinogens; D009602:Nitrosamines; D005557:Formaldehyde; D003903:Deuterium; D006899:Mixed Function Oxygenases; D004128:Dimethylnitrosamine