Glucocorticoid receptors in corticosensitive and corticoresistant thymocyte subpopulations. I. characterization of glucocorticoid receptors and isolation of a corticoresistant subpopulation.
1. Separation of mouse thymocytes by centrifugation on a discontinuous bovine serum albumin gradient leads to the isolation of four subpopulations of cells. 2. The study of I13H]uridine incorporation in vitro by these subpopulation in the presence of steroid shows that one of them is corticoresistant. 3. However, the binding capacity of these subpopulations measured by incubation with [3H]dexamethasone is very similar. 4. It is therefore concluded that in mouse thymus, in contrast with lymphoma cells, corticoresistance may not be explained by a defect of cytoplasmic glucocorticoid receptors.
D000818:Animals; D001667:Binding, Competitive; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D003907:Dexamethasone / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D012710:Serum Albumin, Bovine; D013347:Subcellular Fractions / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D012710:Serum Albumin, Bovine; D003907:Dexamethasone; D014529:Uridine