title: Four-and five-step kinetic models of lactate dehydrogenase.
abstract: A five-step model for the reaction catalyzed by beef heart lactate dehydrogenase (EC 1.1.1.27) reconciles differences observed in the four-step model if pre-steady-state data in the forward direction are compared with similar data in the reverse direction. The relationship between the four-and five-step models indicates what problems can develop when an incomplete model is proposed. Nevertheless, there are advantages to using the less complicated four-step model when comparing the molecular kinetics of enzymes catalyzing the same reaction but obtained from different sources.
mesh_terms: D000818:Animals; D002417:Cattle; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008433:Mathematics; D009206:Myocardium / Q000201:enzymology; D013696:Temperature; D013816:Thermodynamics
chemical_list: D007770:L-Lactate Dehydrogenase