Observations on the antiglobulin tests. II. C4 components on erythrocytes.
This paper describes anti-C4b(ii) antisera shich agglutinate cells from cases of autoimmune haemolytic anaemia, cold-acquired haemolytic anaemia, and certain test cells, using the antiglobulin test. It is suggested that these particular antisera possess activity against C4d absorbed on to erythrocytes. Methods of preparing test cells coated with C3d and C4d are given, together with methods for producing antisera in rabbits active against these components. The significance of these findings is discussed.
D000743:Anemia, Hemolytic / Q000276:immunology; D000744:Anemia, Hemolytic, Autoimmune; D000799:Angioedema / Q000276:immunology; D000818:Animals; D003176:Complement C3 / Q000378:metabolism; D003181:Complement C4 / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism*; D003298:Coombs Test; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007983:Lewis Blood Group Antigens; D011817:Rabbits
D003176:Complement C3; D003181:Complement C4; D007106:Immune Sera; D007983:Lewis Blood Group Antigens; D003165:Complement System Proteins