Isolation of strains of the cellular slime mold Dictyostelium discoideum capable of growing after a single passage in axenic medium.
Wild-type strains of the cellular slime mold Dictyostelium discoideum grow on bacteria. Axenic strains of D. discoideum capable of growing in a simple salts-yeast extract-proteose peptone-glucose medium have been isolated from wild-type strains fo both mating types after a single passage in this medium. This result is consistent with the theory that the axenic phenotype has a simple genetic basis.
D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004023:Dictyostelium / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D008769:Methylnitronitrosoguanidine; D009153:Mutagens; D009154:Mutation; D009235:Myxomycetes / Q000254:growth & development*; D010641:Phenotype; D013172:Spores, Fungal / Q000254:growth & development
D003470:Culture Media; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D003513:Cycloheximide