Effect of UV-irradiation of shifts of energy-rich phosphate compounds: ADP, ATP and AXP in human red blood cells represented by a trigonometrical polynomial.
This paper deals with the effect of UV-irradiation on metabolism of energy rich adenine, esters: ADP, ATP and AXP in human red blood cells in vitro. Preparation, separation and quantitative determination of these compounds were performed by the method of BARTLETT, using a step concentration gradient with an HC1-NH4C1 chloride system. Blood was irradiated in vitro for 30, 60, 90, 120, 150, 180, 210, 240, 270 and 300 min. Mathematical methods based on KOLMAGOTOV's theorem were applied for continuous, not stepwise description of dynamics of changes of ADP, ATP and AXP in red blood cells following UV-irradiation. The obtained curves illustrate dynamic, time-dependent changes of levels of the investigated compounds and point out that the method of BARTLETT used for determination of concentrations of ATP and AXP need further refinements, especially in applications to such biological systems and functional states in which slight shifts of these compounds occur.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D008433:Mathematics; D014466:Ultraviolet Rays
D000227:Adenine Nucleotides; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate