Determination of beta-cetotetrine in plasma and urine using high-performance liquid chromatography with electrochemical detection.
A sensitive and specific method for the determination of beta-cetotetrine in biological fluids has been developed using reversed-phase high-performance liquid chromatography and thin-layer electrochemical detection. The limit of detection for beta-cetotetrine was less than 0.25 pmoles, or approx. 100 pg injected on-column. beta-Cetotetrin could be analyzed in plasma at concentrations of 25ng/ml. Linear responses were observed up to 2.5 mug/ml, and the relative standard deviation of the procedure was +/-5%. The method was also employed for the determination of similar levels of beta-cetotetrine in urine.
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004566:Electrodes; D008832:Microchemistry; D010084:Oxidation-Reduction; D013754:Tetracyclines / Q000032:analysis* / Q000097:blood / Q000652:urine
D013754:Tetracyclines; C000400:chelocardin