[Combined ultrastructural morphometric and biochemical investigations on the rat liver after ligation of the bile duct (author's transl)].
In the present study an ultrastructural morphometric analysis of the rat liver 1, 3 and 7 days after bile duct ligation was performed. Corresponding biochemical investigations included the determination of the cytochrom P 450 contents in the microsomal fraction. In the serum of the rats the activities of GOT and GPT were determined. The quantitative results have shown that the decreased P 450 contents of the microsomal fraction coincided with a considerable increasement of the surface of the smooth endoplasmic reticulum. The smooth endoplasmic reticulum was significantly increased from 1.83 m2/cm3 hepatocytes to 3.49 m2/cm3 hepatocytes after 3 days and remained on this level after 7 days. These results may be considered as quantitative evidence for the term hyperplastic, hypoactive endoplasmic reticulum. - As a second important result our quantitative investigations have shown that the increasement of GLDH activity in the serum especially 1 day after ligation of the bile duct coincided with a signficantly increased mitochondrial volume at this time.
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001699:Biometry; D002779:Cholestasis / Q000201:enzymology / Q000473:pathology; D003135:Common Bile Duct; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005969:Glutamate Dehydrogenase / Q000032:analysis; D008026:Ligation; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D008862:Microsomes, Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000648:ultrastructure; D051381:Rats
D003577:Cytochrome P-450 Enzyme System; D005969:Glutamate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
Vergleichende feinstrukturell-morphometrische und biochemische Befunde an der Rattenleber nach Gallengangsligatur