A biosynthetic method for the preparation of high specific activity 32P-labeled phospholipids.
32P-Labeled phospholipids with specific activities up to 400 mCi/mmole as well as [32P]CDP-choline were prepared by cultivation of mouse fibroblasts or mouse Ehrlich ascites cells in the presence of [32P]orthophosphate. The method was also used to prepare [methyl-3H]choline-labeled glycerophospholipids from [3H]choline. The yields and the specific activities of the phospholipids were significantly lower when preparations of ox white blood cells were used.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002855:Chromatography, Thin Layer; D003566:Cytidine Diphosphate Choline / Q000096:biosynthesis; D004912:Erythrocytes / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D007962:Leukocytes / Q000378:metabolism; D051379:Mice; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis*; D010761:Phosphorus Radioisotopes; D013329:Structure-Activity Relationship; D014316:Tritium
D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D014316:Tritium; D003566:Cytidine Diphosphate Choline