The synthesis of lipids from [1-14C]acetate by human venous endothelium in tissue culture.
Endothelial cells from human umbilical cords were harvested, using a trypsin technique, grown in tissue culture and lipid synthesis studied using [1-14C]acetate as a precursor. Radiosubstrate was incorporated into fatty acids, mono-, di- and triglycerides, cholesterol esters and phospholipids. Radioactivity was also present in the culture medium in the mono-and diglyceride fractions and in the phospholipids running with the solvent front.
D000085:Acetates / Q000378:metabolism*; D002250:Carbon Radioisotopes; D046508:Culture Techniques; D004727:Endothelium / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000096:biosynthesis*; D014471:Umbilical Veins / Q000378:metabolism*
D000085:Acetates; D002250:Carbon Radioisotopes; D008055:Lipids