title: Arabinosyl nucleosides inhibit Toxoplasma gondii and allow the selection of resistant mutants.
abstract: Adenine arabinoside, which inhibits the synthesis of DNA by intracellular Toxoplasma gondii, is more inhibitory to the parasite than it is to cultured human fibroblast host cells. A single-step mutant of T. gondii that is 50-fold more resistant to adenine arabinoside was isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Cytosine arabinoside is notably more inhibitory to cultured human cells than it is to T. gondii. Thus in infected cells treated with cytosine arabinoside and labeled with 3H-deoxyuridine nearly all of the label is specifically incorporated into the intracellular T. gondii.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D003857:Deoxyuridine / Q000378:metabolism; D004351:Drug Resistance; D009154:Mutation; D012313:RNA / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D014122:Toxoplasma / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014740:Vidarabine / Q000494:pharmacology*
chemical_list: D003561:Cytarabine; D012313:RNA; D004247:DNA; D014740:Vidarabine; D013936:Thymidine; D003857:Deoxyuridine