Comparison of human and hamster mitochondrial transfer RNA. Physical properties and methylation status.
We have compared mitochondrial transfer RNA from cultured human (HeLa) and hamster(BHK) cells, giving emphasis to distinctive properties previously noted for the BHK system. Both HeLa and BHK mitochondrial tRNA sedimented slower, and had lower mobilities in "warm" acrylamide gels, than their cytoplasmic counterparts, suggesting that both mitochondrial tRNA populations have unusually loose configurations. HeLa mitochondrial tRNA was found to contain 2.8 methyl groups per 100 nucleotides compared to 8.7 for cytoplasmic, figures similar to previous BHK results. Both mitochondrial tRNA populations were notably deficient in 7-methylguanine, methylribose, and methylated pyrimidines. We propose that mitochondrial tRNA (or perhaps animal mitochondrial tRNA) constitutes a distinct evolutionary class.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003593:Cytoplasm; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D008928:Mitochondria; D012343:RNA, Transfer / Q000378:metabolism*; D012265:Ribonucleotides / Q000032:analysis; D014529:Uridine / Q000378:metabolism
D012265:Ribonucleotides; D012343:RNA, Transfer; D008715:Methionine; D014529:Uridine