The sub-zero temperature chromatographic isolation of transient intermediates of a multi-step cycle: preparation of the substrate-free oxy-ferrous cytochrome P450.
D001665:Binding Sites; D002151:Calorimetry; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification* / Q000378:metabolism; D005615:Freezing; D010100:Oxygen; D011485:Protein Binding; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D003577:Cytochrome P-450 Enzyme System; D010100:Oxygen