[Quantitative determinations of plasma protein fractions in pregnant dairy cows during a 24-hour period using micro-agargel electrophoresis].
Total protein and the socalled classical blood plasma fractions were examined in hourly intervals over 24 hours in ten clinically intact pregnant Jersey cows. The average total protein values recorded from the plasma samples were 6.9 per cent and thus physiologically normal. The arithmetic mean values within one circadian rhythm did not differ markedly within each of the following fractions:albumin (38.7 +/- 5.08) alpha1-globulin (11.22 +/- 2.22),alpha2-globulin (14.25 +/- 1.95), and beta-globulins (12.56 +/- 0.91). Moderate rises were established only for the gamma-globulins at 1 a.m., 7 a.m., and 7 p.m. The protein fractions of blood plasma, consequently, are not subject to any circadian rhythm, and the time of blood sampling, therefore, has no bearings on laboratory diagnosis.
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D002417:Cattle; D002940:Circadian Rhythm; D004587:Electrophoresis, Agar Gel; D005260:Female; D011247:Pregnancy; D011270:Pregnancy, Animal; D013997:Time Factors
D001798:Blood Proteins
Quantitative Bestimmungen der Eiweissfraktionen des Blutplasmas niedertragender Milchkühe im Ablauf eines 24-Stunden-Zeitraumes mit der Mikro-Agarel-Elektrophorese