On the initiation of mammalian RNA polymerase at single-strand breaks in DNA.
Mammalian RNA polymerase B is able to initiate at single-strand breaks in the DNA template. A 3'-OH end at a nick is required for initiation whereas the 5'-end may be either -- OH or phosphate. The 3'-OH group does not function as a primer. An appreciable part of the newly synthesized RNA started at a nick remains associated with the DNA in hydrid form. Initiation of exogeneous RNA polymerase B on chromatin exhibits similar requirements.
D000818:Animals; D002417:Cattle; D004277:DNA, Single-Stranded; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D007700:Kinetics; D008836:Micrococcal Nuclease; D012319:RNA Polymerase II / Q000378:metabolism*; D012260:Ribonucleases; D013698:Templates, Genetic; D013950:Thymus Gland / Q000201:enzymology; D014158:Transcription, Genetic
D004277:DNA, Single-Stranded; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D003851:Deoxyribonucleases; D012260:Ribonucleases; D008836:Micrococcal Nuclease