[Subsequent mutagenesis under the action of UV rays in a culture of Actinomadura carminata, a producer of carminomycin].
The succession of some markers on the chromosomes of Actinomadura carminata was shown with the method of successive mutagenesis based on the preferable effect of the mutagen on DNA in the site of replication. The spore germination was synchronized by selection of the spores of the definite size and maintenance at a temperature of 0 degrees. The periods of formation of the highest numbers of morphological and auxotrophic mutants were compared with the time of DNA replication.
D000192:Actinomycetales / Q000528:radiation effects*; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D002360:Carubicin / Q000096:biosynthesis*; D002470:Cell Survival / Q000528:radiation effects; D003470:Culture Media; D014644:Genetic Variation / Q000187:drug effects; D005827:Genetics, Microbial / Q000528:radiation effects; D008967:Molecular Biology / Q000528:radiation effects; D009154:Mutation / Q000528:radiation effects*; D013171:Spores, Bacterial / Q000528:radiation effects; D013997:Time Factors; D014466:Ultraviolet Rays
D000903:Antibiotics, Antineoplastic; D003470:Culture Media; D002360:Carubicin
Posledovatel'nyĭ mutagenez pod deĭstviem UF-lucheĭ v kul'ture Actinomadura carminata produtsenta karminomitsina