Arginase from human full-term placenta.
Arginase was purified about 1800-fold from extracts of human full-term placenta; the enzyme appeared to be homogenous by disc electrophoresis and molecular-sieve chromatography. The mol. wt. determination by gel filtration and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis yielded a value of 70000 for the most pure and the partially purified enzyme. The human placenta arginase is a metalloenzyme with an optimum pH of 9.1. The Km for L-arginine is 27 mM. L-Ornithine and L-lysine show competitive inhibition with Ki values of 6.3 and 14 mM respectively.
D001119:Arginase / Q000302:isolation & purification*; D002850:Chromatography, Gel; D004789:Enzyme Activation; D006801:Humans; D008345:Manganese; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology*
D008345:Manganese; D001119:Arginase