Sex hormones and metabolism of glycosaminoglycans. I. Effect of orchidectomy and administration of testosterone in rabbits.
The effect of orchidectomy in male rabbits and administration of testosterone to orchidectomized animals on the metabolism of glycosaminoglycans (GAG) has been studied. The response of the different GAG fractions in the aorta varies with the nature of the GAG, and in some cases is different in different segments of the aorta. Orchidectomy produced an increase in hyaluronic acid fraction, decrease in heparin sulphate fraction, and no response in the chondroitin sulphate A fraction in the aortic arch, thoracic aorta, and abdominal aorta. Chondroitin sulphate C and chondroitin sulphate B fractions decreased only in the abdominal aorta and were not significantly altered in the other two segments, while heparin fraction decreased only in the thoracic aorta and was not affected in the other segments. Administration of testosterone to the orchidectomized animals counteracted these changes in the aortic GAG fractures. The enzymes concerned with the synthesis of precursors of GAG--L-glutamine:D-fructose-6-phosphate aminotransferase, UDPG dehydrogenase, and UDPG pyrophosphorylase-- all decreased in the orchidectomized animals; testosterone administration increased their activity in the orchidectomized animals. Enzymes concerned with degradation of GAG--beta-glucuronidase, beta-hexosaminidase, aryl sulphatase, cathepsin, and hyaluronidase--increased in the orchidectomized and decreased on administration of testosterone. Concentration of PAPS and activity of sulphate-activating system and sulphotransferase also decreased in the orchidectomized animals, and testosterone administration tended to restore this decrease to normal levels.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001012:Aorta, Abdominal / Q000378:metabolism; D001013:Aorta, Thoracic / Q000378:metabolism; D001192:Arylsulfatases / Q000378:metabolism; D002369:Castration; D002403:Cathepsins / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism; D011817:Rabbits; D013466:Sulfurtransferases / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000378:metabolism; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000378:metabolism
D006025:Glycosaminoglycans; D013739:Testosterone; D010724:Phosphoadenosine Phosphosulfate; D014533:Uridine Diphosphate Glucose Dehydrogenase; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing); D005957:UTP-Glucose-1-Phosphate Uridylyltransferase; D013466:Sulfurtransferases; D001192:Arylsulfatases; D005966:Glucuronidase; D006821:Hyaluronoglucosaminidase; D000118:Acetylglucosaminidase; D002403:Cathepsins