Stoichiometry of hemolysis by the polyene antibiotic lucensomycin.
The stoichiometry of hemolysis by the polyene antibiotic lucensomycin was investigated. It appears that hemolysis occurs only when a relatively high fraction (probably between 15 and 40%) of the cholesterol sites in the erythrocyte membrane have combined with the polyene. Also in phospholipid-cholesterol vesicles the increase of permeability requires occupancy of 40-50% of the existing cholesterol sites. As for the possible cooperative effect in the hemolytic process, it is probable that several (at least 9-10) lucensomycin-cholesterol adducts must interact on each side of the membrane to form an aqueous channel; the distribution of these adducts in the erythrocyte membrane occurs, however, apparently at random.
D000818:Animals; D000935:Antifungal Agents; D001665:Binding Sites; D002417:Cattle; D002784:Cholesterol / Q000097:blood; D004912:Erythrocytes / Q000648:ultrastructure; D006461:Hemolysis; D007700:Kinetics; D008155:Lucensomycin; D008433:Mathematics; D008563:Membrane Lipids / Q000097:blood; D008567:Membranes, Artificial; D008954:Models, Biological
D000935:Antifungal Agents; D008563:Membrane Lipids; D008567:Membranes, Artificial; D008155:Lucensomycin; D002784:Cholesterol