[Method of localization and evaluation of DNA quantities in bull spermatozoa].
The author attempted to evaluate the DNA quantities by means of a spectrophotometric method of UV light absorption. In a first publication data have been reported on the RNA quantities obtained by this method. This one concerns the data obtained on DNA. By this method, the manipulations on the spermatozoon are more dimple and sources of error are so discarded. It was so possible to get individual data on each spermatozoon, what is possible with chimical methods which give global quantities for a whole sample of semen. It is moreover possible to delimite the localization of DNA and to measure the amounts in each localization. The scale of the obtained data is large enough for information to several workers. The founded quantities and variations in data will be discussed in a following paper.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis*; D008297:Male; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013094:Spermatozoa / Q000032:analysis*
D004247:DNA
Méthode de localisation et d'evaluation des quantités d'A.D.N. contenues dans le spermatozoide de taureau