Radioimmunoassay of iodinated tobramycin.
A radioimmunoassay for tobramycin was developed using antisera from rabbits injected with a tobramycin-bovine serum albumin conjugate. The antibody had a relatively high affinity for (125)I-labeled tobramycin (1.6 x 10(9) liters/mol), thus indicating little interference in immunoreactivity by iodination. The radioimmunoassay was highly specific, and only minor cross-reaction occurred with other aminoglycoside antibiotics. The radioimmunoassay was also more sensitive than previously published tobramycin assays (minimum sensitivity, 280 pg), and there was a significant correlation (P < 0.05) with both the radioenzymatic and microbiological assays.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000918:Antibody Specificity; D002417:Cattle; D006241:Haptens; D007457:Iodine Radioisotopes; D008722:Methods; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine; D014031:Tobramycin / Q000032:analysis*
D000900:Anti-Bacterial Agents; D006241:Haptens; D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine; D014031:Tobramycin