Oxygen-hemoglobin equilibrium of normal and glutathione-deficient sheep.
The affinity of hemoglobin for oxygen did not differ significantly between normal and glutathione-deficient sheep. Although phlebotomy in sheep of both types did not significantly alter the partial pressure values at which hemoglobin is 50% saturated with oxygen, it did increase significantly the erythrocyte glutathione and 2,3-diphosphoglycerate (GSH) and (DPG) concentrations. Because DPG does not bind to sheep hemoglobin, its increase in hypoxic sheep must mean DPG is metabolically controlled in the absence of physiologic effect.
D000818:Animals; D004912:Erythrocytes / Q000378:metabolism; D005978:Glutathione / Q000097:blood / Q000172:deficiency*; D006454:Hemoglobins / Q000032:analysis*; D010100:Oxygen / Q000097:blood*; D010313:Partial Pressure; D012756:Sheep / Q000097:blood*; D012757:Sheep Diseases / Q000097:blood*
D006454:Hemoglobins; D005978:Glutathione; D010100:Oxygen