Differences in L-malate determined enzymatically or titrimetrically in Golden Delicious apples.
In apples, L-malate was significantly less when estimated by titration with NaOH than by spectrophotometry after enzymatic reduction of NAD+ with malate dehydrogenase. The second method would determine L-malate in the form of salts as well as free acid.
D005638:Fruit / Q000032:analysis*; D008291:Malate Dehydrogenase; D008293:Malates / Q000032:analysis*; D008722:Methods; D012972:Sodium Hydroxide
D008293:Malates; D012972:Sodium Hydroxide; D008291:Malate Dehydrogenase