Preparation of a crystalline A1-(2-nitro-4-trimethylammoniophenyl) derivative of bovine insulin.
The reaction of bovine insulin with the hydrophilic reagent 1-fluoro-2-nitro-4-trimethylammoniobenzene iodide at pH 8.2 has been reexamined. From the mixture of products a crystalline derivative substituted at the GlyAl terminus has been isolated. It has been characterized by amino acid analysis, end group analysis, cellulose acetate electrophoresis and sulphitolysis. The derivative has a biological activity (mouse convulsion assay) of 60% relative to insulin.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D003460:Crystallization; D003619:Dansyl Compounds / Q000302:isolation & purification; D004588:Electrophoresis, Cellulose Acetate; D004589:Electrophoresis, Disc; D007328:Insulin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D000644:Quaternary Ammonium Compounds / Q000302:isolation & purification*; D012640:Seizures / Q000139:chemically induced; D015032:Zinc
D003619:Dansyl Compounds; D007328:Insulin; D000644:Quaternary Ammonium Compounds; D015032:Zinc