Complexity and specificity of polysomal poly(A+) RNA in mouse tissues.
Base sequence complexities of polysomal poly(A+)RNA from mouse embryo, brain, and liver have been estimated by hybridization to homologous cDNA to be approximately 7 x 109, 1.5 x 1010, and 7 x109 daltons, respectively. By annealing each cDNA with a large excess of total mouse embryo DNA, the genes coding for the polysomal poly(A+) sequences were shown to be unique. Heterologous hybridization experiments showed that the high abundance class of poly(A+) sequences in one tissue is not identical with the high abundance class in other tissues. However, at least 55%, and possibly more, of the poly(A+) RNA in one tissue is present in the poly(A+) RNA of another tissue.
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D004622:Embryo, Mammalian; D005260:Female; D008099:Liver / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D011061:Poly A / Q000032:analysis*; D011132:Polyribosomes / Q000032:analysis*; D011247:Pregnancy; D012335:RNA, Ribosomal
D012335:RNA, Ribosomal; D011061:Poly A