Separation of chloramphenicol intermediates by high-performance liquid chromatography on micropak-NH2 columns.
A column containing a chemically bonded phase, MicroPak-NH2, was used to seperate six components of a chloramphenicol-intermediate mixture. Two-component eluents comprising a non-hydrogen-bonding apolar solvent (cyclohexane) and a polar hydrogen-bonding solvent (dioxan, tetrahydrofuran or isopropanol) were tested. A correlation was found to exist between the values of the capacity factor (k') and the Hildebrand parameter (delta). Three-component eluents comprising cyclohexane and one of the binary mixtures dioxan-isopropanol or tetrahydrofuran-isopropanol were also studied. Optimum separation conditions with respect to analysis time, degree of resolution and detection limit were established. The system was successfully used to analyse production samples.
D002701:Chloramphenicol / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008832:Microchemistry; D012997:Solvents
D012997:Solvents; D002701:Chloramphenicol