Increased detection and yield of adenovirus in caffeine-stabilised KB cell monolayers.
Viable monolayers of KB cells were maintained without passage for up to one year by adding 2 X 10(-3) M caffeine to the medium, while untreated monolayers degenerated after 2 weeks. When adenoviruses type 2 and 17 were titrated on caffeine-stabilised KB cells, the late breakthrough of cytopathic effect at terminal dilutions (up to 40 days after inoculation) resulted in titre determinations up to 100-fold higher than those measured before spontaneous degeneration in untreated cells. Yields of adenovirus type 2 in caffeine-treated KB cultures infected at different multiplicities and harvested at different intervals were equal to, or up to 100-fold higher than those obtained in untreated cultures.
D000260:Adenoviruses, Human / Q000254:growth & development / Q000302:isolation & purification*; D002110:Caffeine / Q000494:pharmacology; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D014776:Virus Cultivation; D014779:Virus Replication
D002110:Caffeine