Partial purification of nucleopeptides from rabbit skeletal muscle by the tannin-caffeine procedure.
After precipitation of acid-soluble proteins from sulfosalicylic acid extracts of rabbit skeletal muscles with tannin at neutral pH, the remaining material still formed insoluble complexes with tannin. After removing tannin with caffeine complexes subjected to column chromatography on Dowex 1 x 8 gave four fractions containing both amino acids and nucleotides. It was concluded that the isolated material contains nucleopeptides, besides nucleotides.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002852:Chromatography, Ion Exchange; D009132:Muscles; D009698:Nucleoproteins / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*; D011817:Rabbits; D013634:Tannins / Q000494:pharmacology*; D014020:Tissue Extracts / Q000032:analysis*
D000596:Amino Acids; D009698:Nucleoproteins; D010455:Peptides; D013634:Tannins; D014020:Tissue Extracts; D002110:Caffeine