Electron capture detection of an apomorphine heptafluorobutyrate derivative at low picogram levels.
An electron capturing derivative of apomorphine was prepared by incubating the drug with heptafluorobutyric anhydride (HFBA), triethylamine and heat. Mass spectral analysis suggests that HFBA reacts with both phenolic hydroxyl groups on apomorphine to give a derivative detectable at low picogram levels. This method is sufficiently sensitive for pharmacokinetic studies in the horse and is likely applicable to other dopaminergic analogues of apomorphine.
D000818:Animals; D001058:Apomorphine / Q000031:analogs & derivatives* / Q000097:blood* / Q000138:chemical synthesis; D002849:Chromatography, Gas; D006736:Horses / Q000378:metabolism*; D013058:Mass Spectrometry; D008722:Methods
D001058:Apomorphine