[Chronic infection of diploid cells of human embryo musculocutaneous tissue].
A model of chronic influenza infection of a strain of human embryo skin-muscle tissue diploid cells (KM WSN) was developed. When the cells were propagated without medium changes, they regularly produced virus in low infectious titres and hemagglutinins in titers of 1 : 2 to 1 : 4. Frequent medium changes caused more intensive infectious virus reproduction with no or very poor hemagglutinin accumulation. Superinfection of KM WSN cultures even with frequent medium changes led to a marked reduction in the amount of infectious virus production with no detectable interferon. The WSN KM virus was found to differ from the initial variant by a number of markers. The observed pattern of virus production suggests a mechanism of persistence in this system in connection with the interfering effect of the defective virus produced by the cells.
D002478:Cells, Cultured; D003673:Defective Viruses; D004171:Diploidy; D006389:Hemagglutinins, Viral; D006801:Humans; D009980:Influenza A virus; D009132:Muscles / Q000196:embryology; D012867:Skin / Q000201:enzymology; D014776:Virus Cultivation / Q000379:methods
D006389:Hemagglutinins, Viral
Khronicheskaia infektsiia diploidnykh kletok kozhno-myshechnoĭ tkani émbriona cheloveka