title: A study of calf-thymus histone H2B using 13C magnetic resonance.
abstract: Calf thymus histone H2B has been studied by means of 13c magnetic resonance. Most sidechain resonances have been assigned. Analysis of 13c peak intensities by a simple computer-aided method proves that 13c magnetic resonance spectroscopy can be used to monitor aggregation phenomena more accurately than 1H magnetic resonance or other physico-chemical methods. Re-examination of 1H spectral simulations shows substantial agreement with 13C results. It is concluded that in 0.1M added NaCL the H2b aggregates include the chain segment from 50 (+/-5) to 108 (+/-5).
mesh_terms: D000818:Animals; D002417:Cattle; D003201:Computers; D006657:Histones; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D011485:Protein Binding; D011487:Protein Conformation; D012965:Sodium Chloride; D013950:Thymus Gland / Q000032:analysis*
chemical_list: D006657:Histones; D046911:Macromolecular Substances; D012965:Sodium Chloride