title: Lectin-receptor interactions in liposomes. II. Interaction of wheat germ agglutinin with phosphatidylcholine liposomes containing incorporated monosialoganglioside.
abstract: Bovine brain gangliosides incorporated into phospholipid liposomes provide receptors for wheat germ agglutinin. Purified monosialogangliosides were mixed with egg phosphatidylcholine, and unilamellar liposomes were generated. Addition of wheat germ agglutinin induced the liposomes to fuse, and gel filtration analysis revealed that the lectin was incorporated into the fused liposomes. The fusion process was studied by following the changes in the 90 degrees light scattering. Increasing the proportion of the monosialoganglioside in the liposomes was found to increase both the extent of the lectin-induced liposome fusion and the rate of the reaction; below a threshold of approx. 5 mol%, the process was extremely slow. The increase in light scattering could be prevented by the addition of the hapten inhibitor, N-acetyl-D-glucosamine (1 mM). Addition of the inhibitor, subsequent to the lectin, caused a partial decrease in light scattering due to the dissociation of unfused vesicle aggregates. Electron microscopic examination revealed that the ganglioside-containing liposomes were vesicles, 244 +/- 25 A (S.D.) in diameter. Upon addition of wheat germ agglutinin, the vesicles appeared to fuse to form larger vesicles, corresponding to dimers and trimers of the initial vesicles. Inhibition studies with a variety of monosaccharides indicated that the sialic acid moieties present in the ganglioside acted as the lectin-receptor sites. This was confirmed by the observation that wheat germ agglutinin did not interact with phosphatidylcholine vesicles containing desialyated ganglioside.
mesh_terms: D001665:Binding Sites; D001667:Binding, Competitive; D005732:Gangliosides; D006601:Hexoses; D007700:Kinetics; D037102:Lectins; D008027:Light; D008081:Liposomes; D008854:Microscopy, Electron; D008954:Models, Biological; D010713:Phosphatidylcholines; D037121:Plant Lectins; D012542:Scattering, Radiation; D012639:Seeds; D012794:Sialic Acids; D013050:Spectrometry, Fluorescence; D014908:Triticum
chemical_list: D005732:Gangliosides; D006601:Hexoses; D037102:Lectins; D008081:Liposomes; D010713:Phosphatidylcholines; D037121:Plant Lectins; D012794:Sialic Acids