Effects of an ionophore, X-537A, on mouse CNS cultures: a light and electron microscope study.
The effects of the ionophore X-537A on organotypic CNS tissue culture were investigated with bright field and electron microscopy. In addition to condensation of mitochondria this agent was found to induce a relatively selective vacuolation of the Golgi apparatus of neurons and glial cells. At the dosages used (50 mug/ml for 2-3 hours and 100 mug/ml for 1 hour) these changes were irreversible. Additional experiments suggested that the cellular effects of X-537A were primarily due to its ability to translocate Na+ across cellular membranes.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D046508:Culture Techniques; D006056:Golgi Apparatus / Q000187:drug effects; D007832:Lasalocid / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects; D009457:Neuroglia / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000648:ultrastructure; D009836:Oligodendroglia / Q000187:drug effects* / Q000648:ultrastructure; D011149:Pons; D012154:Reticular Formation / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D012964:Sodium / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D012964:Sodium; D002118:Calcium; D007832:Lasalocid