A method for the determination of hyaluronate in the presence of other glycosaminoglycans and its application to human intervertebral disc.
A highly sensitive proteoglycan-binding assay was developed for the detection and determination of small amounts of hyaluronate. Application of this assay to human intervertebral-disc tissue showed that the radial distribution of hyaluronate closely followed the distribution of proteoglycans.
D000368:Aged; D001665:Binding Sites; D002675:Child, Preschool; D002809:Chondroitin Sulfates / Q000032:analysis; D002850:Chromatography, Gel; D003094:Collagen / Q000032:analysis; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis*; D007403:Intervertebral Disc / Q000032:analysis*; D007632:Keratan Sulfate / Q000032:analysis; D008297:Male; D008722:Methods; D011509:Proteoglycans
D006025:Glycosaminoglycans; D011509:Proteoglycans; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D003094:Collagen; D007632:Keratan Sulfate