Influence of serum protein and medium composition on the dynamic adhesiveness of lymphocytes and erythrocytes.
The dynamic adhesiveness of lymphocytes and erythrocytes to glass was studied in two media differing in concentration of serum protein and bivalent cations (Mg2+, Ca2+). The serum protein reduced adhesion of both kinds of cells, the effect being much greater with erythrocytes. Different adhesive properties of lymphocytes and erythrocytes under dynamic conditions were also observed in the protein-free media. Erythrocytes appear to be more sensitive to the influence of serum proteins as well as to the medium composition (in the absence of serum). Some influence of the ionic strength of the medium on erythrocyte adhesion is suggested. No effect of bivalent cations on lymphocyte adhesiveness with and without protein was observed.
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D002448:Cell Adhesion; D004912:Erythrocytes; D008214:Lymphocytes; D008274:Magnesium / Q000378:metabolism*; D051381:Rats; D012996:Solutions; D013997:Time Factors
D001798:Blood Proteins; D002413:Cations, Divalent; D012996:Solutions; D008274:Magnesium; D002118:Calcium