[In vitro biosynthesis of cholesterol and its precursors in the osseous tissue of rats].
By thin-layer chromatography compounds of sterol origin were isolated from the nonsaponified fraction of bone tissue of rats. The occurrence of squalene, lanosterol, cholesterol and incompletely identified Cdelta2427-sterol in the organic matrix of the bone was demonstrated by means of UV- and IR-spectrometry. Bone tissue incubation with 1,2-C14-aceticacid yielded C14-labeled lanosterol, C28, 29-sterols, cholesterol and squalene. The rate of cholesterol synthesis in the bone tissue was lower than that in the liver and intestine of rats.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D002784:Cholesterol / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007810:Lanosterol / Q000096:biosynthesis; D051381:Rats; D013185:Squalene / Q000096:biosynthesis; D013261:Sterols / Q000096:biosynthesis
D000085:Acetates; D013261:Sterols; D007810:Lanosterol; D013185:Squalene; D002784:Cholesterol
Issledovanie biosinteza kholesterina i ego predshestvennikov v kostnoĭ tkani krys in vitro