Splenic imaging with 99mTc-labeled erythrocytes: a comparative study of cell-damaging methods.
Several methods of damaging red blood cells (RBCs) for splenic imaging were compared to determine the optimum approach. The RBCs from donor animals were labeled with 99mTcO4- and damaged by heat, excess acid citrate dextrose (ACD), excess Sn(II) ion, or the sulfhydryl inhibitors N-ethylmaleimide (NEM) or p-hydroxymercuribenzoate (PMB). The organ distributions of undamaged and damaged RBCs were determined in rats, and splenic imaging studies were performed in rabbits. Splenic deposition and spleen-to-liver ratios with heat- or sulfhydryl-damaged 99mTc-RBCs were significantly greater (p less than 0.001) than the values obtained using ACD or Sn(II) ion. Heat-damaging produces good splenic localization of 99mTc-RBCs but requires rigidly controlled incubation conditions. NEM-damaging provides an excellent and predictable alternative approach.
D000818:Animals; D002951:Citrates; D004912:Erythrocytes / Q000187:drug effects; D005033:Ethylmaleimide; D005947:Glucose; D006358:Hot Temperature; D006902:Hydroxymercuribenzoates; D008297:Male; D011817:Rabbits; D011877:Radionuclide Imaging / Q000379:methods*; D051381:Rats; D013154:Spleen; D013667:Technetium; D014001:Tin
D002951:Citrates; D006902:Hydroxymercuribenzoates; D013667:Technetium; D014001:Tin; D005947:Glucose; D005033:Ethylmaleimide