[Nature and fractionation of barley proteins extracted by ethanol, isopropanol and n-propanol in different proportions].
Barley alcoholsoluble protein extractabilities by aqueous ethanol, isopropanol and n-propanol were measured at room temperature. The quantities extracted by each alsohol strongly depend on the concentration of the alcohol. The most efficient concentration for the three  alcohols were by increasing order: 45 per cent ethanol, 40 per cent isopropanol, 35 per cent (w/w) n-propanol. Hrodein preparations extracted by these three alcoholic solutions and by 75 per cent (w/w) ethanol were compared by means of the flour nitrogen percentage they contain and by electrophoresis, amino-acid analysis and Sephadex G 100 gel filtration. The preparations studied do not differ markedly in their amino-acid composition or electrophoretic pattern which shows at least 17 different bands. On the other hand, Sephadex G 100 gel filtration separates two main groups of proteins, The first one is present at the same level in all preparations studied and consists of electrophoretically typical hordeins (already described hordeins). The other group represents a fraction of the preparation, the more abundant as the solvent is more effective. This second group is excluded on Sephadex G 100 chromatography and does not give well defined bands by starch gel electrophoresis. Consequently it is related to some glutelins. Nevertheless its amino-acid composition is very close to the mean hordein composition. Electrophoretic comparison with glutelins extracted by acetic acid and with hordeins, all reduced and alkylated, discloses a great similitude between this fraction, the glutelins and some hordein fast components alpha, beta and gamma.
D000433:1-Propanol; D000596:Amino Acids / Q000032:analysis; D004592:Electrophoresis, Starch Gel; D000431:Ethanol; D001467:Hordeum / Q000032:analysis; D008722:Methods; D010940:Plant Proteins / Q000302:isolation & purification
D000596:Amino Acids; D010940:Plant Proteins; D000431:Ethanol; D000433:1-Propanol
Nature et fractionnement des protéines de l'Orge extraites par l'éthanol, l'isopropanol et le n-propanol à des titres différents-19h