Contribution to the study of excision repair following UV irradiation in mammalian cells cultivated in vitro.
The absence of pyrimidine dimer excision after UV irradiation in rat cell line was confirmed. The possibility of excision repair system depression by acidification of medium with H3PO4 in HeLa cells has been proved. The results obtained by two different methods of deproteinization and DNA isolation are in good conformity.
D002460:Cell Line; D002725:Chloroform; D004247:DNA / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects; D006367:HeLa Cells; D010756:Phosphoric Acids / Q000494:pharmacology; D011402:Pronase; D011740:Pyrimidine Dimers / Q000378:metabolism*; D014466:Ultraviolet Rays
D010756:Phosphoric Acids; D011740:Pyrimidine Dimers; D002725:Chloroform; D004247:DNA; D011402:Pronase