Lipid synthesis by perfused lung.
An isolated lung ventilated with pulses of negative pressure and perfused through the pulmonary vasculature was utilized for the study of 3-sn-phosphatidylcholine synthesis. The perfusion fluid consisted of a Krebs-Ringer phosphate buffer with 6% bovine serum albumin, pH 7.4, and the appropriate substrate. The simultaneous incorporation of (1-14C) palmitate and (2-3H) glycerol and the simultaneous incorporation of (CH3-14C) choline and (CH3-3H) methionine were examined. From these experiments it is concluded: 1) lung tissue incorporates (2-3H) glycerol into 3-sn-phosphatidylcholine to a greater extent than any other lipid examined; 2) both choline and methionine contribute to the synthesis of 3-sn-phosphatidylcholine, and 50-70% of the label in its nitrogen base is derived from choline and 30-50% from methionine; and 3) a high PO2 appears to reduce the synthesis of 3-sn-phosphatidylcholine.
D000818:Animals; D002794:Choline / Q000378:metabolism; D006224:Cricetinae; D004075:Diglycerides / Q000096:biosynthesis; D005260:Female; D005990:Glycerol / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008168:Lung / Q000378:metabolism*; D008647:Mesocricetus; D008715:Methionine / Q000378:metabolism; D010169:Palmitic Acids / Q000378:metabolism; D010477:Perfusion; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis; D051381:Rats; D013045:Species Specificity; D014280:Triglycerides / Q000096:biosynthesis
D004075:Diglycerides; D008055:Lipids; D010169:Palmitic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D014280:Triglycerides; D008715:Methionine; D002794:Choline; D005990:Glycerol