Relative ribosomal RNA cistron multiplicity in oocytes and postembryonic stages of the eutelic nematode Panagrellus silusiae.
The number of ribosomal RNA cistrons has been measured in the total DNA extracted from L2 juvenile and adult stages of the free-living nematode Panagrellus silusiae. Saturation hybridization studies with homologous rRNA indicate that both stages have about 275 ribosomal genes per haploid equivalent. Using homologous 125I-labelled rRNA for in situ hybridization, the mean number of silver grains per DNA content for oocyte, hypodermis and gut nuclei was similar. The mean DNA contents of maturing oocyte, hypodermis and gut nuclei are about 20C, 2C, and 10C respectively. We conclude that rDNA amplication alone is insufficient to account for the variation in DNA content of oocytes and that postembryonic development in this eutelic organism occurs without a significant differential increase in the number of ribosomal cistrons per worm.
D000818:Animals; D004247:DNA / Q000378:metabolism; D005260:Female; D005796:Genes; D009348:Nematoda / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*
D012335:RNA, Ribosomal; D004247:DNA