title: Action of prolactin on ornithine decarboxylase activity in mammary gland explants of mice.
abstract: Prolactin stimulated ornithine decarboxylase activity in mammary gland explants from midpregnant mice. The enhanced enzyme activity occurred in explants which were preincubated for 1 day in medium containing insulin, hydrocortisone, insulin plus hydrocortisone, or in medium containing no hormones. The largest prolactin effect was observed in tissues which were pretreated with insulin plus hydrocortisone; a greater than ten-fold increase in ornithine decarboxylase activity was observed when these tissues were incubated with prolactin for 2 hours. An effect of prolactin on ornithine decarboxylase activity was also observed in explants prepared from lactating mouse mammary glands.
mesh_terms: D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006854:Hydrocortisone / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007774:Lactation; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011247:Pregnancy; D011388:Prolactin / Q000494:pharmacology*
chemical_list: D007328:Insulin; D011388:Prolactin; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D006854:Hydrocortisone