title: Autodegradation of pre-mRNA containing nuclear ribo-nucleoprotein particles. The effect of autodegradation on the double-stranded RNA sequences and on the protein composition of particles.
abstract: The quantitative changes of double-stranded RNA components of nuclear ribonucleo-protein particles containing pre-mRNA was investigated in the course of incubation of particles at 37 degrees C. The incubation of purified nuclear particles revealed the fragmentation of long double-stranded RNA sequences into shorter stretches. The presence of nuclear sap in the incubation mixture resulted in degradation of the double-stranded RNAs into acid soluble products. Autodegradation and/or ribonuclease treatment of nuclear RNP particles is accompanied by quantitative changes in the minor protein constituents of informofer.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002850:Chromatography, Gel; D008297:Male; D008970:Molecular Weight; D009698:Nucleoproteins / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D012260:Ribonucleases; D012261:Ribonucleoproteins / Q000378:metabolism
chemical_list: D009698:Nucleoproteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D012260:Ribonucleases