title: Cellular and subcellular distribution of the S-100 protein in rabbit and rat central nervous system.
abstract: The cellular and subcellular distribution of the S-100 protein in rabbit and rat central nervous system was studied both quantitatively and qualitatively. Microcomplement fixation estimations on bulk-prepared neuronal and glial cells showed at least five to six times higher amounts of water-soluble S-100 in glial cell-enriched fractions as compared to fractions enriched in neuronal perikarya. Nerve cell fractions contained a higher percent of tissue-bound S-100 protein. High levels of S-100 protein were found in mitochondria and soluble protein fractions. Immunoelectron microscope investigations demonstrated S-100 protein in neuronal structures, such as the postsynaptic membrane and part of the plasma membrane. Among glial cells the astrocytic filaments contained high levels of S-100 protein. S-100 was also found in most subcellular membranes of astrocytes and oligodendrocytes.
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000648:ultrastructure; D001923:Brain Chemistry; D002415:Cats; D002467:Cell Nucleus / Q000032:analysis; D005260:Female; D008297:Male; D008861:Microsomes / Q000032:analysis; D008928:Mitochondria / Q000032:analysis; D009185:Myelin Proteins / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009457:Neuroglia / Q000032:analysis; D009474:Neurons / Q000032:analysis / Q000648:ultrastructure; D009836:Oligodendroglia / Q000648:ultrastructure; D011817:Rabbits; D009418:S100 Proteins / Q000032:analysis*; D013569:Synapses / Q000648:ultrastructure; D013574:Synaptosomes / Q000032:analysis
chemical_list: D009185:Myelin Proteins; D009419:Nerve Tissue Proteins; D009418:S100 Proteins