The preparation of human hemoglobin alpha-subunit and a study of its monomer-dimer association.
An improved procedure for the isolation of the alpha-subunit of human hemoglobin is described. The monomer-dimer equilibrium in alpha-subunit solutions has been studied by boundary analysis in gel filtration, sedimentation velocity, sedimentation equilibrium, and cross-linking with dimethyl adipimidate. A dissociation constant has been determined from the sedimentation equilibrium data. The reaction with haptoglobin of cross-linked alpha-subunit showed that the dimer fraction wound form a stable complex.
D000596:Amino Acids / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins / Q000302:isolation & purification; D006801:Humans; D046911:Macromolecular Substances; D008433:Mathematics; D008970:Molecular Weight; D011485:Protein Binding; D013438:Sulfhydryl Compounds
D000596:Amino Acids; D006454:Hemoglobins; D046911:Macromolecular Substances; D013438:Sulfhydryl Compounds