[Enzyme activity of citrate, glyoxylate and pentosephosphate cycles during synthesis of citric acids by Candida lipolytica].
The activity of key enzymes of the citrate and glyoxylate cycles was compared in yeast cells during intensive synthesis of citric acids and in its absence in the course of growth of Candida lipolytica on glucose ("glucose" yeast cells) and on hexadecane ("hexadecane" yeast cells). Citrate and isocitrate were found to be formed by the yeast in the tricarboxylic acid cycle. The ability of the yeast for "overproduction" of citrate and isocitrate during its growth on glucose and hexadecane depends on the high activity of the key enzyme of cycle, citrate synthase, as compared with the activity of other enzymes of the tricarboxylic acid cycle. Citrate predominated among excreted acids during growth on glucose in conditions of nitrogen deficiency while isocitrate prevailed during growth on hexadecane. The predominating synthesis of citrate in the first case seems to be related to a lower activity of aconitase in the "glucose" cells as compared with the "hexadecane" cells.
D000154:Aconitate Hydratase / Q000378:metabolism; D000473:Alkanes / Q000378:metabolism; D002175:Candida / Q000201:enzymology / Q000378:metabolism*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D002951:Citrates / Q000096:biosynthesis*; D002952:Citric Acid Cycle; D005947:Glucose / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D007522:Isocitrate Lyase / Q000378:metabolism; D007523:Isocitrates / Q000378:metabolism; D008190:Lyases / Q000378:metabolism*; D008292:Malate Synthase / Q000378:metabolism; D010428:Pentosephosphates / Q000378:metabolism
D000473:Alkanes; D002951:Citrates; D006038:Glyoxylates; D007523:Isocitrates; D010428:Pentosephosphates; D007521:Isocitrate Dehydrogenase; D002950:Citrate (si)-Synthase; D008292:Malate Synthase; D008190:Lyases; D007522:Isocitrate Lyase; D000154:Aconitate Hydratase; D005947:Glucose
Aktinost' fermentov tsitratnogo, glioksilatnogo i pentozofosfatnogo tsiklov pri sinteze limonnykh kislot Candida lipolytica