The use of the IgG fraction of typing sera in the lymphocytotoxic test: a comparison with other cytotoxic methods.
The application of a simple batch method for the isolation of IgG from weakly lymphocytotoxic sera is described. The use of purified IgG as an antibody source at the serum concentration or up to tentimes that level resulted in the conversion of 25 out of 28 previously weak or non-reactive cytotoxic sera into potentially useful tissue typing reagents. The sensitivity of this technique was assessed by comparison with five cytotoxic test systems.
D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D006650:Histocompatibility Testing; D006801:Humans; D007074:Immunoglobulin G
D007074:Immunoglobulin G