Chemical transformation of Chinese hamster cells: II. Appearance of marker chromosomes in transformed cells.
The chromosomes of 12 samples of cultured Chinese hamster kidney and prostate cells (4 normal and 8 transformed), whose tissue culture properties have already been described (Kirkland, 1976) have been examined for numerical change and for the appearance of abnormal markers. Six transformed kidney subclones contained a consistent telocentric marker not present in the normal parental cell, and Giemsa banding demonstrated this to be the centromere and the long (q) arm of the number 4 chromosome in all cases. Two transformed prostate subclones also contained a consistent telocentric marker, not present in similarly derived normal subclones or in the normal parental cell, and Giemsa banding demonstrated this to be a different fragment (the centromere and most of the p arm) of the number 4 chromosome. It is believed that the use of a mixed-serum culture medium, designed to stabilize the karyotype of cultured Chinese hamster cells, is at least partly responsible for the detection of these transformation-associated chromosome changes.
D002273:Carcinogens / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D003470:Culture Media; D007621:Karyotyping; D007668:Kidney / Q000648:ultrastructure; D008297:Male; D011467:Prostate / Q000648:ultrastructure
D002273:Carcinogens; D003470:Culture Media