title: [Preparation of granulocyte concentrates from healthy donors using a continuous-flow centrifuge (CFC)].
abstract: By means of continous flow centrifuge the authors have obtained suspensions of granulocytes from the blood of healthy donors. Results of granulocytophereses are presented here with the main points in the therapy of granulocytopenic patients with granulocytes rich suspension. The mean volume of the obtained suspension was 418ml. and it contained 9,78 X 10(9) leukocytes. 70 percent of them were granulocytes, the said volume contained 48 ml of red blood cells. All the donors had been premedicated with i.v. dexamethason (4mg./kg body weight). No one of the 51 donors developed anaemia or hypoproteinemia. After the application of the protamin sulphate, however, two donors had face flushing breathlessness and gastric ache. These symptoms disappeared after the administration of corticosteroids. Although they are connected with the administration of protamin sulphate we can not explain them.
mesh_terms: D001782:Blood Donors; D001803:Blood Transfusion; D002498:Centrifugation / Q000379:methods*; D006098:Granulocytes; D006801:Humans; D007938:Leukemia / Q000628:therapy; D007962:Leukocytes
vernacular_title: Priprema granulocitnih koncentrata of zdravih davaoca protocnom centrifugom (CFC)