Preparation and evaluation to Clostridium septicum toxoid.
Clostridium septicum was grown under controlled conditions to produce high levels of alpha toxin. The toxin was toxoided and concentrated by ultrafiltration. The toxoided alpha toxin retained immunizing properties. The concentrated toxoid, when formulated on Total Combining Power, in monovalent or multivalent clostridial products, provided protection to rabbits against direct challenge with Cl. septicum spores. The toxoided alpha toxin produced antitoxin levels which exceeded current minima.
D000818:Animals; D000992:Antitoxins / Q000032:analysis; D001427:Bacterial Toxins / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000302:isolation & purification / Q000592:standards; D003013:Clostridium / Q000276:immunology*; D051379:Mice; D011817:Rabbits; D014121:Toxoids / Q000008:administration & dosage / Q000302:isolation & purification / Q000592:standards*
D000992:Antitoxins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D014121:Toxoids