[O2-consumption and CO2 formation by kidney cortex sections incubated in an optimal substrate mixture].
Basing on the knowledge of renal metabolism a substrate mixture has been developed which appears to be promising for the preservation of this organ. Slices of rat kidney were incubated in different solutions, and the oxygen consumption and 14CO2 formation were determined at 6 degrees C after 4 and 9 hrs. In the substrate mixture the rat kidney cortex shows a continual O2 consumption, which could be increased by more than twice the amount compared with the values obtained after incubation without substrate. The proportion of fatty acid and amino acid mixture supplied amounts to 90% of the total CO2 formation by the renal cortex. Under the conditions selected, the incubation mixture allows one to spare endogenic substrates. The criteria of O2 consumption and CO2 formation alone are not sufficient to decide whether the functioning of the mitochondria and thus the energy supply are ensured under these conditions. Further studies are necessary to solve this problem.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000096:biosynthesis*; D005227:Fatty Acids / Q000378:metabolism; D007672:Kidney Cortex / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats
D000596:Amino Acids; D005227:Fatty Acids; D002245:Carbon Dioxide
O2-Verbrauch und CO2-Bildung durch Nierenrindenschnitte bei Inkubation in einem optimierten Substratgemisch