[Study of the structural lability of biomembranes and their components by the fluorescent analysis method. IV. Kinetic characteristics of the binding of unsaturated fatty acids with bovine serum albumin].
By studying fluorescent parameters of ionic and neutral probes the kinetics of complex formation between the molecules of bovine serum albumin (BSA) and unsaturated fatty acids was investigated. The following regularities were observed: 1. Unlike the saturated fatty acids the unsaturated ones changed fluorescent parameters of the probes according to the cooperative mechanism, significantly decreasing the quantum yield values; 2. Temperature dependence of the fluorescence of both probe types in the complexes BSA -- oleic acid and BSA -- linole acid shows some bends at t=30--39 degrees C for which the lipid components is responsible.
D000817:Anilino Naphthalenesulfonates; D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D005453:Fluorescence; D008041:Linoleic Acids; D009281:Naphthalenes; D009829:Oleic Acids; D011485:Protein Binding; D012710:Serum Albumin, Bovine; D013696:Temperature
D000817:Anilino Naphthalenesulfonates; D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D009281:Naphthalenes; D009829:Oleic Acids; D012710:Serum Albumin, Bovine
Issledovanie strukturnoĭ labil'nosti biomembran i ikh komponentov metodom fluorestsentnogo analiza. IV. Kineticheskie osobennosti sviazyvaniia nepredel'nykh zhirnykh kislot s molekulami syvorotochnogo al'bumina byka