An assay for albumin messenger RNA in an vitro protein synthesizing system from wheat germ.
The synthesis of serum albumin in an in vitro protein-synthesizing system from wheat germ stimulated with rat liver polysomal RNA is demonstrated by immunoprecipitation. The newly synthesized albumin has the same electrophoretic mobility as rat serum albumin. There is a linear increase in precursor incorporation into total protein and albumin with increasing RNA concentration. Potassium and magnesium optima for albumin synthesis are different from those for total protein synthesis.
D000818:Animals; D007930:Leucine / Q000378:metabolism; D008099:Liver; D008274:Magnesium / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism; D051381:Rats; D012709:Serum Albumin / Q000096:biosynthesis*; D014908:Triticum / Q000378:metabolism*
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012709:Serum Albumin; D007930:Leucine; D008274:Magnesium; D011188:Potassium