The activity of non-specific esterase in the thyroid epithelial cells of the guinea pig as influenced by various inhibitors and activators. A histochemical study.
The action of various inhibitors and activators upon esterase activity in the thyroid epithelial cells is demonstrated. The agents used were triorthocresylphosphate (TOCP), parachloromercuribenzoate (PCMB), Arsanillic acid, p-nitrophenyl dimethyl carbamate and bis p-nitrophenyl phosphate. TOCP was found to inhibit selectively the activity in the follicle cells proper when alpha naphthyl acetate was used as a substrate. Arsanillic acid (0,001 M) activated the follicle cells proper selectively, but if the concentration was raised to 0,01 M the effect was that of inhibition while the activity in the para-, inter- and intrafollicular cells was unchanged. The results obtained are related to previous biochemical and histochemical observations and the nature of esterases in the thyroid is discussed.
D000115:Acetylesterase / Q000032:analysis; D000818:Animals; D002219:Carbamates / Q000494:pharmacology; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004950:Esterases / Q000032:analysis* / Q000037:antagonists & inhibitors; D006168:Guinea Pigs; D006651:Histocytochemistry; D013961:Thyroid Gland / Q000201:enzymology*; D014317:Tritolyl Phosphates / Q000494:pharmacology
D002219:Carbamates; D002729:Chloromercuribenzoates; D014317:Tritolyl Phosphates; D004950:Esterases; D000115:Acetylesterase