Effects of handling and etherization on incorporation of [3H] lysine into protein of mouse brain and liver.
A standardized handling experience of 20 sec duration elevated the amount of radioactive lysine incorporated into brain total proteins during a 10 min labeling period begun either 10 min or 30 min after the handling. Etherization for 20 sec produced similar metabolic changes. Incorporation of [3H] lysine into liver proteins was minimally affected by handling, while slightly altered by etherization. The metabolic changes detected in the brain after handling did not appear to be side-effects of changes in blood-borne radioactivity. The results indicate that laboratory stresses often thought to be of minor importance can have large effects on ordinary assays of protein metabolism in brain.
D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004986:Ether / Q000494:pharmacology*; D005019:Ethyl Ethers / Q000494:pharmacology*; D006234:Handling, Psychological; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009928:Organ Specificity; D014176:Protein Biosynthesis / Q000187:drug effects
D001798:Blood Proteins; D005019:Ethyl Ethers; D009419:Nerve Tissue Proteins; D004986:Ether; D008239:Lysine