An improved separation procedure for nucleoside monophosphates on polyethyleneimine-(PEI-)cellulose thin layers.
A procedure is described for the two-dimensional separation of the 4 major and 16 modified nucleoside-(5') monophosphates on anion-exchange thin layers of polyethyleneimine- (PEI-)cellulose. The method, which is simple and less time-consuming than existing partition chromatographic methods, may be used for the identification of 5'-termini of RNA and RNA fragments.
D002852:Chromatography, Ion Exchange / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D009843:Oligoribonucleotides / Q000032:analysis; D012313:RNA; D012265:Ribonucleotides / Q000302:isolation & purification*
D009843:Oligoribonucleotides; D012265:Ribonucleotides; D012313:RNA