Simultaneous analysis of catecholamine acidic metabolites by single ion monitoring and manual control of the magnetic field.
A method for multicomponent analysis by single ion monitoring is presented. This method involves monitoring of one ion per selected compound in a GC run. To focus sequentially on each ion, the magnetic field strength of the mass spectrometer is manually reset to the proper value during the intervals between eluting peaks. Stationary phase bleeding provides a suitable reference background for rapid location of selected ions. This technique has been applied to the simultaneous analysis of 3,4-dihydroxyphenylacetic acid (DOPAC), vanillylmandelic acid (VMA) and homovanillic acid (HVA) in urine samples. Quantitative data for DOPAC and HVA are presented using 3,4-dihydroxyphenylpropionic acid (DHPPA) as an internal standard.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000652:urine; D002395:Catecholamines / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006719:Homovanillic Acid / Q000652:urine; D006801:Humans; D008280:Magnetics; D008297:Male; D014642:Vanilmandelic Acid / Q000652:urine
D002395:Catecholamines; D015102:3,4-Dihydroxyphenylacetic Acid; D014642:Vanilmandelic Acid; D006719:Homovanillic Acid