Ultraviolet mutagenesis in Streptomyces coelicolor: induction of reversions in a polyauxotrophic strain.
UV irradiation of the aerial spores of Streptomyces coelicolor pro arg hom cys strain A677 causes the induction of Arg+ and Cys+ revertants. Both phenotypic classes of revertants are induced according to approximately dose-squared kinetics. Some 30% of the induced Cys+ revertants were shown by direct genetic tests to be due to suppressor mutations. Indirect evidence, based on the fact that a high proportion of the UV-induced Arg+ revertants were also simultaneously Hom+, indicates that suppressors are probably also responsible for the Arg+ phenotype of many revertants.
D001120:Arginine / Q000378:metabolism; D003553:Cystine / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D009154:Mutation; D013171:Spores, Bacterial / Q000528:radiation effects; D013302:Streptomyces / Q000378:metabolism / Q000528:radiation effects*; D013489:Suppression, Genetic; D014466:Ultraviolet Rays
D003553:Cystine; D001120:Arginine