Detection of an antigen in albumin subjected to repeated heating.
Stabilized 5 and 25% normal serum albumin (human) derived from plasma, placentas and plasma-placental blends was subjected to repeated heating at 56 degrees C for 120 h, interspersed with storage at 4 degrees C for 48 h. Immunoelectrophoretic analyses showed that after the ninth heating, 5% plasma albumin developed a component which migrated in the alpha-globulin region and gave a reaction of nonidentity with albumin. This antigen, which was not detected in the other albumin samples, reacted with antiserum prepared against control 5% plasma albumin, indicating that it was present in the latter at an undetectably low concentration.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D007122:Immunoelectrophoresis; D011247:Pregnancy; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology*; D013997:Time Factors
D000941:Antigens; D012709:Serum Albumin