High resolution polyacrylamide gradient gel electrophoresis.
A method was developed for high resolution electrophoresis of proteins in linear gradient (3 to 30%) polyacrylamide gel rods in a neutral phosphate buffer containing 0.1% sodium dodecyl sulfate. Well-defined protein zones were observed and improved resolution was attained especially for low molecular weight proteins in preparations containing a variety of polypeptides, e.g. viruses that are often separated by continuous gel methods. Electropherograms of continuous (8%) and gradient (3 to 30%) gels were made of purified vesicular stomatitis virus, variola virus, Rickettsia rickettsii, and alpha and beta chains of hemoglobin in order to demonstrate the resolution of the gradient system.
D000178:Acrylamides; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D013045:Species Specificity; D014764:Viral Proteins / Q000302:isolation & purification
D000178:Acrylamides; D010455:Peptides; D011506:Proteins; D014764:Viral Proteins