A restriction endonuclease from Staphylococcus aureus.
A specific endonuclease, Sau 3AI, has been partially purified from Staphylococcus aureus strain 3A by DEAE-cellulose chromatography. The enzyme cleaves adenovirus type 5 DNA many times, SV40 DNA eight times but does not cleave double-stranded phi X174 DNA. It recognizes the sequence (see article) and cleaves as indicated by the arrows. Evidence is presented that this enzyme plays a role in the biological restriction-modification system of Staphylococcus aureus strain 3A.
D001483:Base Sequence; D004247:DNA; D004262:DNA Restriction Enzymes / Q000302:isolation & purification / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D008970:Molecular Weight; D013211:Staphylococcus aureus / Q000201:enzymology*
D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes