Immobilization of enzymes based on hydrophobic interaction. III. Adsorbent substituent density and its impact on the immobilization of beta-amylase.
Hexyl-groups have been introduced into crosslinked Sepharose 6B, yielding gels with degrees of substitution which range from 0.02 to 0.70 mol hexyl-side chain per mole galactose residue. The gels were exposed to beta-amylase in solution, and the resulting adsorbates indicated a monotonic increase in adsorption capacity with an increasing hexyl-content. Adsorbate activity, by contrast, displayed a maximum for a carrier gel with a hexyl-galactose ratio of 0.51. Adsorbates based on gels with different hexyl-content were used in column reactors for continuous maltose production from a soluble starch substrate.
D000327:Adsorption; D000478:Alkylation; D000681:Amylases; D004800:Enzymes, Immobilized; D005782:Gels; D011134:Polysaccharides; D011506:Proteins / Q000032:analysis; D012685:Sepharose / Q000031:analogs & derivatives; D014867:Water
D004800:Enzymes, Immobilized; D005782:Gels; D011134:Polysaccharides; D011506:Proteins; D014867:Water; D012685:Sepharose; D000681:Amylases