The biosynthesis of transfer RNA in insects. II. Isolation of transfer RNA precursors from the posterior silk gland of Bombyx mori.
The occurrence of precursors to tRNA in the post-polysomal fraction of the posterior silk gland of Bombyx mori was demonstrated by pulse-chase labeling and DNA-RNA hybridization competition experiments. These precursors had molecular sizes ranging from 4S to 5S on polyacrylamide gel electrophoresis. Analysis of the incorporation of the methyl group from [methyl-14C]methionine revealed that a radioactive peak on polyacrylamide gel appeared in the 4.5S region during brief labeling. This suggested that some methylation occurred at the 4.5S precursor step.
D012831:Bombyx / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009694:Nucleic Acid Precursors / Q000302:isolation & purification*; D012313:RNA / Q000096:biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis* / Q000302:isolation & purification; D014529:Uridine / Q000378:metabolism
D009694:Nucleic Acid Precursors; D012313:RNA; D012343:RNA, Transfer; D008715:Methionine; D014529:Uridine