Isolation of suppressor sensitive mutants in the Ai gene of phage lambda.
Previous experiments have shown that mutations in the Ai gene can suppress the growth defect of lambda N- phages. Many temperature resistant derivatives of phage lambdatsN9 have been isolated and among these 5 have been found which are Ai- and have an amber suppressible behaviour. These mutants can help in defining the role of the Ai gene in phage lambda development.
D003090:Coliphages / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D005092:Exonucleases / Q000378:metabolism; D005809:Genes, Regulator; D008040:Genetic Linkage; D006358:Hot Temperature; D008242:Lysogeny; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines / Q000494:pharmacology; D010641:Phenotype; D013489:Suppression, Genetic
D009153:Mutagens; D009604:Nitrosoguanidines; D005092:Exonucleases