Isolation of rat transferrin using CNBr-activated sepharose 4B.
1. The isolation of transferrin from rat serum by means of affinity chromatography on CNBr-activated Sepharose 4 B is described. -2. Subfractionation by isoelectric focusing yielded two transferrin fractions with identical biological behaviour but with small differences in isoelectric point (6.0 and 5.8) and sialic acid contents.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D003488:Cyanogen Bromide; D007525:Isoelectric Focusing; D051381:Rats; D012685:Sepharose; D012794:Sialic Acids / Q000032:analysis; D014168:Transferrin / Q000032:analysis / Q000302:isolation & purification*
D000596:Amino Acids; D012794:Sialic Acids; D014168:Transferrin; D012685:Sepharose; D003488:Cyanogen Bromide