Long-term patency of vein grafts preserved in liquid nitrogen in dimethyl sulfoxide.
Autogenous canine jugular veins were stored in 15% dimethyl sulfoxide (DMSO) in liquid nitrogen vapor for one to 28 days and then implanted in the carotid artery as autografts. The patency rate at one year was 62.5-87.5%. The patency rate of fresh jugular vein autografts placed in the carotid artery for one year was 75%. Similar autografts stored in liquid nitrogen vapor for one to 28 days without the cryopreservative DMSO exhibited a zero to 12.5% patency rate at one year. Scanning electron microscope studies revealed preservation of theendothelium in DMSO protected veins and a damaged or sloughed endothelium in veins frozen without DMSO cryopreservation.
D000818:Animals; D002339:Carotid Arteries / Q000601:surgery; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage*; D004727:Endothelium / Q000166:cytology; D005260:Female; D005615:Freezing; D006085:Graft Survival / Q000187:drug effects; D007601:Jugular Veins / Q000166:cytology / Q000637:transplantation*; D008297:Male; D008855:Microscopy, Electron, Scanning; D009584:Nitrogen; D009926:Organ Preservation / Q000379:methods*; D012965:Sodium Chloride; D014021:Tissue Preservation / Q000379:methods*; D014182:Transplantation, Autologous
D012965:Sodium Chloride; D009584:Nitrogen; D004121:Dimethyl Sulfoxide