Preparation of delipidized serum protein for use in cell culture systems.
A rapid procedure for the preparation of delipidized serum protein is described. The delipidized protein can be used for the maintenance and growth of tissue culture cells in a lipid-free environment. The extraction procedure greatly reduces all serum lipid classes and the delipidized protein supports the growth of a variety of cells in culture.
D001798:Blood Proteins / Q000302:isolation & purification*; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D008722:Methods
D001798:Blood Proteins; D003470:Culture Media