Further studies on the mechanism of adrenaline-induced lipolysis in lipid micelles.
Lipase [EC 3.1.1.3] depleted lipid micelles, in which lipolysis was not elicited by adrenaline, were prepared from lipid micelles. When these lipase-depleted lipid micelles incubated with adipose tissue extract containing lipase activity, adrenaline-induced lipolysis was restored to almost the same level as that of native lipid micelles. Adrenaline-induced lipolysis was not restored when the lipase-depleted lipid micelles were homogenized or sonicated. Various tissue extracts from kidney, lung, liver, and pancreas, and post-heparin plasma, which contained lipase activity, restored adrenaline-induced lipolysis in lipase-depleted lipid micelles.
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D003102:Colloids / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D008823:Micelles / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism; D010740:Phospholipases / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D014357:Trypsin / Q000494:pharmacology
D003102:Colloids; D008823:Micelles; D011092:Polyethylene Glycols; D010740:Phospholipases; D008049:Lipase; D014357:Trypsin; D004837:Epinephrine