X-ray microanalysis of ultrathin frozen and freeze-dried sections of human sperm cells.
X-ray microanalysis of air dried and ultrathin frozen and frozen dried sections of human sperm cells has indicated a large deviation of elemental composition between cells in a single sample and between samples. Analysis of air dried cells indicates a similar subcellular elemental distribution to that found in sectioned material. Sodium to potassium ratios are found to be similar in both preparations. Air drying is considered a valid method for the preparation of sperm cells for analysis.
D004577:Electron Probe Microanalysis; D005612:Freeze Drying; D005629:Frozen Sections; D006801:Humans; D008297:Male; D008867:Microtomy; D011188:Potassium / Q000032:analysis; D012964:Sodium / Q000032:analysis; D013094:Spermatozoa / Q000032:analysis* / Q000648:ultrastructure; D014965:X-Rays
D012964:Sodium; D011188:Potassium