Changes in labeling of soluble and solubilized hippocampus proteins after a learning experiment in rats.
At various intervals after acquisition of a brightness discrimination in rats labeled leucine was intraventricularly applied. Hippocampus tissue was fractionated in soluble and solubilized insoluble protein fractions. Protein content and labeling of several electrophoretically resolved bands showed a biphasic time course: a first increase was observed 20 minutes after training including preferably soluble proteins, whereas a second increase (about eight hours after training) was mainly related to solubilized insoluble proteins.
D000818:Animals; D006624:Hippocampus; D007858:Learning; D007930:Leucine / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D051381:Rats; D012995:Solubility
D009419:Nerve Tissue Proteins; D007930:Leucine