Synthesis and loss of activity of glucose-6-phosphate dehydrogenase in dividing plant cells.
1. Using the technique of density-labelling with deuterium oxide, evidence has been obtained for the de novo synthesis of glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate:NADPH+ 1-oxidoreductase, EC 1.1.1.49), during the culture of synchronously growing plant cells. 2. The entire increase in enzyme activity during the early cell cycles in this material can be accounted for by the appearance of an enzyme species with increased buoyand density. 3. A method is described for resolving overlapping distribution profiles after density centrifugation, which allows estimation of the amount of each species present at different times, and calculation of the loss of activity of the light species present from the start of culture. 4. Loss of activity of glucose-6-phosphate dehydrogenase in normal growing conditions in the presence of 2,4-dichlorophenoxyacetic acid is very much faster than in conditions which do not lead to cell division: in the absence of 2,4-dichlorophenoxyacetic acid, or in the presence of the inhibitor of RNA synthesis, 6-methylpurine.
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003201:Computers; D003903:Deuterium; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis / Q000378:metabolism*; D008433:Mathematics; D010944:Plants / Q000201:enzymology*; D011687:Purines / Q000494:pharmacology; D013997:Time Factors
D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; D003903:Deuterium; D005954:Glucosephosphate Dehydrogenase