Effects of analogues of prostaglandins E2 and F2alpha on uterine luminal fluid accumulation in the estrogen-treated ovariectomized rat: an indirect measure of cervical tone.
Experiments were performed to determine if prostaglandins were able to reduce cervical tone in the rat. Cervical tone was assessed indirectly by measuring uterine luminal fluid accumulation in ovariectomized rats implanted subcutaneously with Silastic capsules containing crystalline estradiol-17beta. When given subcutaneously in separate experiments, 16,16-dimethyl-prostaglandin E2, methyl ester, and 15(S)-15-methyl-prostaglandin F2alpha, analogues of prostaglandins E2, AND F2alpha, respectively, caused the loss of uterine luminal fluid. Fluid accumulation in uterine horns ligated at the cervical end did not differ in control and treated rats, whereas in non-ligated horns the prostaglandin analogues reduced fluid accumulation, suggesting the cervix as their site of action. For both prostaglandin analogues, the effects on uterine luminal fluid accumulation were seen within 45 min of administration and were related to the dose administered. The effects of submaximal doses of the analogues were additive. These results suggest that prostaglandins are able to reduce cervical tone in the estrogen-treated rat.
D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D002584:Cervix Uteri / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D010053:Ovary / Q000502:physiology; D011312:Pressure; D011458:Prostaglandins E / Q000008:administration & dosage / Q000494:pharmacology*; D011460:Prostaglandins F / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D014590:Uterine Contraction / Q000187:drug effects*
D011458:Prostaglandins E; D011460:Prostaglandins F; D004958:Estradiol