[A highly sensitive C-terminal specific radioimmunoassay for human parathormone as a routine method (author's transl)].
The basis for the radioimmunoassay of parathormone (PTH) as a routine method is a new sheep antiserum and a labelled PTH stabilised by a modification of the purification technique. The antiserum is obtained by immunisation with pig and cattle parathormone, it is C-terminal specific and is used in the assay in a final dilution of 1:35000. The affinity to human PTH is markedly greater than of the antisera used up to now. Two purification steps of 125J labelled bovine PTH lead to a tracer with a nonspecific binding of approximately 5% which increases to approximately 10% within 6 weeks. All normal sera investigated so far were measurable quantitatively (normal range 0.7 to 2.5 mul/equiv.). The lower sensitivity range was at 0.3 mul/equiv. All patients with chronic renal insufficiency and dialysis patients have an increased PTH concentration (3.9 to greater than 20 mul/equiv.). This also applies to patients with primary hyperparathyroidism (2.9 to greater than 20 mul/equiv.).
D000818:Animals; D002417:Cattle; D006041:Goats; D006801:Humans; D006961:Hyperparathyroidism / Q000097:blood; D007457:Iodine Radioisotopes; D007676:Kidney Failure, Chronic / Q000097:blood; D010281:Parathyroid Hormone / Q000097:blood*; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods*; D006435:Renal Dialysis; D012756:Sheep / Q000276:immunology; D013552:Swine; D013997:Time Factors
D007457:Iodine Radioisotopes; D010281:Parathyroid Hormone
Hochsensitiver, C-terminal-spezifischer Radioimmunoassay für menschliches Parathormon als Routinemethode