On the nature of the difference in the densities of the particles of two tobamoviruses.
Particles of sunnhemp mosaic virus (SHMV) are denser than those of the U2 strain of tobacco mosaic virus (T2MV) when their densities are estimated by equilibrium centrifugation in gradients of either cesium chloride or Metrizamide; in cesium chloride the densities are 1.318 and 1.307 g/ml, and in Metrizamide they are 1.249 and 1.240 g/ml. Experiments with particles reassembled from homologous or heterologous mixtures of the RNAs and coat proteins of the viruses show that the difference in their densities is determined by their coat proteins. The disassembled coat proteins of the two viruses have the same density, but polymerized SHMV protein is less dense than polymerized T2MV protein. Particles reassembled from homologous or heterologous mixtures of the RNAs and coat proteins of the viruses have the density of the nucleoprotein particles used as the source of protein. The density difference of the two virus nucleoproteins therefore reflects the different behavior of the two proteins on assembly with RNA.
D002499:Centrifugation, Density Gradient; D009029:Mosaic Viruses / Q000032:analysis*; D010942:Plant Viruses / Q000032:analysis*; D011487:Protein Conformation; D012367:RNA, Viral / Q000032:analysis; D014027:Tobacco Mosaic Virus / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis
D012367:RNA, Viral; D014764:Viral Proteins