A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.
A modified procedure for the isolation of a nuclear pore complex-lamina fraction from rat liver nuclei is described. Evidence is provided that the isolated lamina, a 150-A thick, proteinaceous structure, apposes the inner nuclear envelope membrane, connecting nuclear pore complexes and surrounding the entire nucleus.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus / Q000648:ultrastructure*; D002712:Chlorides / Q000494:pharmacology; D004247:DNA / Q000032:analysis; D008094:Lithium / Q000494:pharmacology; D008099:Liver / Q000648:ultrastructure*; D008274:Magnesium / Q000494:pharmacology; D008566:Membranes / Q000187:drug effects / Q000648:ultrastructure; D009698:Nucleoproteins / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D012313:RNA / Q000032:analysis; D051381:Rats; D013010:Sonication
D002712:Chlorides; D009698:Nucleoproteins; D010743:Phospholipids; D012313:RNA; D004247:DNA; D008094:Lithium; D008274:Magnesium