Inhibition by amiloride of sodium-dependent fluid reabsorption in the rat isolated caudal epididymis.
The rate of fluid reabsorption was studied in the rat isolated caudal epididymal sac in vitro. 2 Part of the fluid reabsorption was found to be dependent on intraluminal Na+. Amiloride (0.1 mM) completely inhibited this component of fluid reabsorption. 3 The log dose-inhibition curve to amiloride was sigmoid and the IC50 value was found to be 1.6 muM.
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D003864:Depression, Chemical; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D012964:Sodium / Q000494:pharmacology
D011719:Pyrazines; D000584:Amiloride; D012964:Sodium