Cesium chloride gradients of chromatin after treatment with micrococcal nuclease.
Cesium chloride equilibrium density centrifugation shows that treatment of rat liver nuclei with low concentrations of micrococcal nuclease for extremely short periods of time results in the appearance of chromatin fractions of low protein/DNA ratio and even free DNA. The DNA of these chromatin fractions is shorter than the DNA moiety of one chromatin subunit. The amount of high buoyant density material is decreased with increasing digestion time. We conclude that this material belongs to the minor chromatin fraction which is not organized according to the subunit model.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D003851:Deoxyribonucleases; D004591:Electrophoresis, Polyacrylamide Gel; D011489:Protein Denaturation; D051381:Rats
D002843:Chromatin; D004247:DNA; D003851:Deoxyribonucleases