title: A radioimmunoassay for growth hormone release-inhibiting hormone: method and quantitative tissue distribution.
abstract: A specific antiserum has been produced to haemocyanin conjugaed synthetic growth hormone release inhibiting hormone (GHRIH). This has allowed the development of a radioimmunoassay for GHRIH sensitive to 5 pg/tube. GHRIH content in 2 m acetic acid extracts of rat tissues have been measured and show the majority to be CNS-especially hypothalamus and septum and preoptic areas with substantial amounts in spinal cord and thalamus. Extra neurological localization in pancreas, gastric antrum, colon and thyroid have been demonstrated.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001923:Brain Chemistry; D002845:Chromatography; D007031:Hypothalamus / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D013004:Somatostatin / Q000032:analysis*; D013116:Spinal Cord / Q000032:analysis
chemical_list: D013004:Somatostatin