The translation in vitro of mRNA from developing cysts of Artemia salina.
Successive stages in the development of the brine shrimp cyst were used as a model for studying differentiation at the level of mRNA transcription and translation. The poly (A)-containing mRNA from dormant cysts and free-swimming larvae (nauplii) was found to be efficiently translated in a wheat-germ cell-free system, and electrophoretic patterns of translation products in vitro resembled those of the endogenous proteins extracted from the equivalent developmental stages. Each stage, however, exhibits a characteristic protein pattern. Two low-molecular-weight proteins prominent in the cyst disappeared almost completely in the nauplius stage, whereas the proportion of actin increased 3-fold. Parallel patterns were observed upon translation in vitro of the respective mRNA preparations. The percentage of the acidic protein, tubulin, decreased somewhat during development.
D000199:Actins / Q000096:biosynthesis; D000205:Actomyosin / Q000096:biosynthesis; D000818:Animals; D002846:Chromatography, Affinity; D003078:Colchicine / Q000378:metabolism; D033364:Decapoda / Q000378:metabolism*; D005260:Female; D008970:Molecular Weight; D010063:Ovum / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism; D014404:Tubulin / Q000096:biosynthesis; D014747:Vinblastine
D000199:Actins; D012333:RNA, Messenger; D014404:Tubulin; D011061:Poly A; D014747:Vinblastine; D000205:Actomyosin; D003078:Colchicine