The subcellular distribution of carotenoids in Phycomyces blakesleeanus C115 car-42 mad-107 (--).
1. Subcellular fractions of the C115 car-42 mad-107(--) strain of Phycomyces blakesleeanus, prepared by differential centrifugation, were irreversibly associated with extraneous lipids. Such contamination was avoided by the use of density gradient centrifugation. 2. The two beta-carotene-containing fractions, a particulate fraction and lipid globules, also contained sterol, phospholipid and alkaline p-nitrophenylphosphatase activity. Both fractions revealed close similarities on amino acid analysis.
D000596:Amino Acids / Q000032:analysis; D002338:Carotenoids / Q000032:analysis*; D005658:Fungi / Q000032:analysis*; D006867:Hydrolases / Q000032:analysis; D008055:Lipids / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010800:Phycomyces / Q000032:analysis*; D013261:Sterols / Q000032:analysis; D013347:Subcellular Fractions / Q000032:analysis
D000596:Amino Acids; D008055:Lipids; D010743:Phospholipids; D013261:Sterols; D002338:Carotenoids; D006867:Hydrolases