Changes in cardiac and hepatic glucocorticoid receptors after adrenalectomy.
1. The total number of specific dexamethasone-binding sites in rat heart and liver cytosol was measured at intervals after adrenalectomy. 2. Between 12 and 48 h after adrenalectomy there was a significant increase in the number of binding sites in both heart and liver cytosol. Affinity was unchanged. 3. Of the [3H]corticosterone bound to liver cytosol proteins after an intravenous injection 98% disappeared within 2 h in vivo. Dissociation of endogenous corticosterone-receptor complexes in liver cytol will thus be substantially complete some hours before the number of receptors increases. 4. It was concluded that there is a true increase in the number of glucocorticoid receptors occurring principally between 12 and 48 h after suppression of endogenous steroids.
D000315:Adrenalectomy; D000818:Animals; D003345:Corticosterone / Q000378:metabolism / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D003907:Dexamethasone / Q000378:metabolism; D005260:Female; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D009206:Myocardium / Q000648:ultrastructure*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000187:drug effects; D011987:Receptors, Steroid / Q000187:drug effects; D013997:Time Factors
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone; D003345:Corticosterone