Activity in saline of phthalylated or succinylated derivatives of mycobacterial water-soluble adjuvant.
A water-soluble fraction (WSA) of the cell wall can substitute for mycobacterial cells in Freund complete adjuvant. However, when WSA is administered in saline instead of in a water-in-oil emulsion, its adjuvant activity is very weak, and under certain experimental conditions it can even inhibit the humoral immune response. The data reported in the present study show that after treatment by phthalic or succinic anhydride the adjuvant activity of WSA was markedly changed, since high levels of circulating antibodies were produced when these derivatives were administered with an antigen in an aqueous medium. Moreover, the antigenic determinants of WSA were modified and acylated WSA had no tuberculin-like activity.
D000215:Acylation; D000276:Adjuvants, Immunologic; D000812:Anhydrides / Q000378:metabolism; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000937:Antigen-Antibody Reactions; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007252:Influenza Vaccines / Q000494:pharmacology; D008297:Male; D051379:Mice; D009161:Mycobacterium / Q000276:immunology*; D010795:Phthalic Acids / Q000378:metabolism*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D012965:Sodium Chloride; D012995:Solubility; D013386:Succinates / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D000812:Anhydrides; D000907:Antibodies, Bacterial; D007252:Influenza Vaccines; D010795:Phthalic Acids; D013386:Succinates; D012710:Serum Albumin, Bovine; D012965:Sodium Chloride