The effect of various stains on quantitative agarose-gel electrophoresis: evaluation of the results with the aid of an analog computer.
The effect of dyes of varying purity and with different binding mechanisms on quantitative electrophoresis was examined with the aid of agarose-gel electrophoresis, the phoretogram curves being evaluated with an analog computer. Concurrent quantitative results were obtained with the three dyes tested, viz. Amido Black 10 B, Ponceau Red S and Procion Brilliant Blue. It is concluded that no falsification of electrophoresis results will be caused by dyes with colour-producing secondary components.
D000580:Amido Black; D001798:Blood Proteins / Q000032:analysis*; D004396:Coloring Agents; D003202:Computers, Analog; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans
D001798:Blood Proteins; D004396:Coloring Agents; D000580:Amido Black