[Arylsulfatase activity in the pineal body, studied by electron microscopy].
For the demonstration of aryl-sulphatase in the corpus pineale as a substrat p-Nitrochatecholsulphat (2-Hydroxy-5-nitrophenyl sulphat) has been used. The reaction is negativ when p-Nitrophenylsulphat or acetylphenylsulphat are used. Localization of the enzyme occurred in the Golgi-system and in the smooth-surface endoplasmic reticulum. By the aid of the histochemical reaction the formation of primary lysosomes can be demonstrated on the level of the Golgi system and of the smooth-surfaced endoplasmic reticulum as well. Aryl-sulphatase correspond to the: A and B type. Reaction in the presence of pCMB and KCN is inhibited.
D000818:Animals; D001192:Arylsulfatases / Q000032:analysis*; D005260:Female; D006056:Golgi Apparatus / Q000201:enzymology; D008297:Male; D010870:Pineal Gland / Q000201:enzymology*; D051381:Rats; D013429:Sulfatases / Q000032:analysis*
D013429:Sulfatases; D001192:Arylsulfatases
Aril-szulfatáz aktivitás elektronmikroszkópos vizsgálata corpus pinealeban