The purification of porcine haptoglobin by affinity chromatography with concanavalin A-Sepharose.
Methods designed for the isolation of human haptoglobin (Hp) were found insufficient when applied to pig plasma due to the formation of a material tentatively identified as albumin dimer. Small scale separation is possible by preparative polyacrylamide gel electrophoresis. Larger scale purification from nonglycoprotein contaminants such as albumin dimer is achieved by affinity chromatography using immobilized concanavalin A. Porcine haptoglobin is microheterogeneous. More than 14 components, partially resolveable by gel filtration, were detected.
D000818:Animals; D002846:Chromatography, Affinity; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D006242:Haptoglobins / Q000302:isolation & purification*; D008970:Molecular Weight; D012685:Sepharose; D013552:Swine
D006242:Haptoglobins; D003208:Concanavalin A; D012685:Sepharose