2-Deoxyglucose selectively inhibits Fc and complement receptor-mediated phagocytosis in mouse peritoneal macrophages II. Dissociation of the inhibitory effects of 2-deoxyglucose on phagocytosis and ATP generation.
Macrophages incubated in 2-deoxy-D-glucose (2-dG)-containing medium showed a marked decrease in cellular ATP content, and were unable to ingest IgG- and complement-coated erythrocytes via the corresponding membrane receptors for these ligands. However, the inhibitory effects of 2-dG on Fc- and C3 receptor-mediated phagocytosis were not a consequence of lowered macrophage ATP levels since addition of glucose or mannose to the culture medium restored the capacity of the macrophages to ingest IgG- and C3-coated particles without increasing ATP levels. These results indicate that Fc- and C3 receptor-mediated phagocytosis (opsonin dependent) differs qualitatively from the ingestion of latex and zymosan particles (opsonin independent); they suggest that the same regulatory molecules govern the responses of phagocytic cells to signals initiated by both the Fc and C3 receptors. The possibility that these molecules are regulated by glycosylation is discussed.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D001665:Binding Sites / Q000187:drug effects; D003176:Complement C3 / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism*; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D007141:Immunoglobulin Fc Fragments; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D009895:Opsonin Proteins; D010587:Phagocytosis / Q000187:drug effects*; D013864:Thioglycolates
D003176:Complement C3; D003837:Deoxy Sugars; D007141:Immunoglobulin Fc Fragments; D009895:Opsonin Proteins; D013864:Thioglycolates; D000255:Adenosine Triphosphate; D003165:Complement System Proteins; D003847:Deoxyglucose