[Recovery of continuous cells following prolonged storage at the temperature of liquid nitrogen].
The recovery process of HeLa continuous cells after prolonged storage at nitrogen liquid temperature (--196 degrees C) has been analysed. The karyologic analysis evidence indicates a high stock cell karyostability. A study of cell division, generation cycle parameters and DNA synthesis by autoradiography demonstrated cell adaptation to a continuous passage to be due to the increase of proliferative pool and to the intensitification of generation processes in the multiplicated population. Cell structure changes during adaptation were reversible.
D002467:Cell Nucleus / Q000648:ultrastructure; D002875:Chromosomes; D005615:Freezing; D006367:HeLa Cells / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D008938:Mitosis; D009584:Nitrogen; D014021:Tissue Preservation
D009584:Nitrogen
Issledovanie vosstanovleniia perevivaemykh kletok posle dlitel'nogo khraneniia pri temperature zhidkogo azota