Mouse factor B of the alternative pathway of complement activation. I. Purification, characterization, and functional behavior.
Mouse factor B was purified and a monospecific antiserum was raised. The physicochemical data of this protein (108,000 m.w., 5.9 S, 5.9 to 6.05 isoelectric point) were determined. The functional behavior resembles that of human and guinea pig factor B and it operates efficiently in the C3 feedback cycle of the alternative pathway of complement activation. A provisional scheme is given for the operation of the C3bB enzyme on the cellular (macrophage) level.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003176:Complement C3 / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism*; D006041:Goats; D006168:Guinea Pigs; D007106:Immune Sera / Q000302:isolation & purification; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D014357:Trypsin / Q000494:pharmacology
D001798:Blood Proteins; D003176:Complement C3; D007106:Immune Sera; D003165:Complement System Proteins; D014357:Trypsin