[Production of immobilized alpha-amylase and its properties].
Active immobilized alpha-amylase was obtained when applying AE-cellulose chromatography and glutaric dialdehyde. The time of the enzyme interaction with the carrier, amount of glutaric dialdehyde necessary for binding, optimal enzyme: carrier ratio as well as the methods for desiccation of the immobilized amylase preparations were specified. Conditions are selected for alpha-amylase stabilization with the presence of glutaric aldehyde. Immobilized amylase as compared to free enzyme is shown to be more pH-stable in the acid and alkaline zones of pH (2.0-3.5 and 10.5-12.0), thermostable (within a temperature range of 20-60 degrees C) and resistant to the effect of 5.5 M urea.
D000681:Amylases; D001236:Aspergillus oryzae / Q000201:enzymology; D002384:Catalysis; D004789:Enzyme Activation; D004800:Enzymes, Immobilized; D006358:Hot Temperature
D004800:Enzymes, Immobilized; D000681:Amylases
Oderzhannia immobilizovanoï alpha-smilazy ta ïï vlastyvosti