[Isoenzyme characteristics of glucose-6-phosphage dehydrogenase and lactate dehydrogenase of continuous cells from the collection of the Sverdlovsk Research Institute of Virus Infections].
An analysis of isoenzymes of glucose-6-phosphage dehydrogenase (G-6-P-DH) and lactage dehydrogenase (LDH) of continuous cells from the collection of Sverdlovsk Research Institute of Virus Infections was carried out. Seventeen continous human and animal cell lines were examined by electrophoresis in a vertical block of 7% polyacrylamide gel followed by histochemical detection of the enzymes. The HEp-2, HEp-2 clone No. 23, KB, RH, and FL cells were shown to have the electrophoretic motility of G-6-PDG characteristic of HeLa cell line. LEP and SOC cell lines were contaminated with mouse cells. A culture of rat fibroblasts had isoenzymes of G-6-PDG and LDG characteristic of HeLa cell line. The remaining cell lines had the isoenzymatic characteristics corresponding to their species appurtenance.
D000818:Animals; D002460:Cell Line; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D013045:Species Specificity; D014586:USSR
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase
Izofermentnaia kharakteristika gliukozo-6-fosfatdegidrogenazy i laktatdegidrogenazy perevivaemykh kletok kollektsii Sverdlovskogo nauchno-issledovatel'skogo instituta virusnykh infektsii