Specific radioimmunochemical identification and quantitation of hemoglobins A2 and F.
Hyperimmune antisera to chromatographically purified hemoglobins F and A2 were produced in rabbits and made specific for the immunogen by adsorption with normal human hemoglobin A conjugated to cyanogen bromide-activated agarose. A radioimmunoassay was established that permitted identification and quantitation of each of these two minor hemoglobins in hemolysates containing other hemoglobin components. The quantities of hemoglobins A2 and/or F present in hemolysates of individuals with beta-thalassemia, sickle cell anemia, Hb-C disease, and other hematological disorders were determined immunochemically, and the results were commpared to values obtained by microcolumn chromatography for the measurement of Hb-A2 or with the alkali denaturation technique in quantitating Hb-F. The immunoassay procedure has a greater sensitivity than other commonly employed techniques and can detect as little as 0.05 mug of these hemoglobins.
D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D004306:Dose-Response Relationship, Immunologic; D005319:Fetal Hemoglobin / Q000032:analysis*; D006441:Hemoglobin A / Q000032:analysis*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D011817:Rabbits; D011863:Radioimmunoassay; D013789:Thalassemia / Q000276:immunology
D006454:Hemoglobins; D006441:Hemoglobin A; D005319:Fetal Hemoglobin