Vesicle interactions with antibody and peptide hormone: role of vesicle composition.
Artificial lipid vesicles (artificial membranes) were shown to bind human 125I-antithyroglobulin (anti-Tg) and human 125I-thyrotropin. Vesicles made with gangliosides bound more antibody and hormone than vesicles lacking them. These gangliosides contained a variety of carbohydrates including glucose, galactose, N-acetyl-galactosamine, and sialic acid. The in vivo stability of antibody-vesicle complexes was a function of vesicle composition: vesicles were most stable when formed from phosphatidylcholine, cholesterol, and gangliosides. Anti-Tg-vesicle complexes bind to thyroglobulin, indicating that at least some of the antibody associated with the vesicle still retains ability to bind to its specific antigen. The addition of a specific antibody or hormone to artificial lipid vesicles may serve as a mechanism to confer specificity to the vesicle in vivo.
D000906:Antibodies; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008081:Liposomes; D011485:Protein Binding; D013954:Thyroglobulin / Q000276:immunology*; D013972:Thyrotropin
D000906:Antibodies; D007457:Iodine Radioisotopes; D008081:Liposomes; D013972:Thyrotropin; D013954:Thyroglobulin