Isolation of a specific antigen with alkaline phosphatase activity from soluble extracts of Paracoccidioides brasiliensis.
A specific antigen of Paracoccidioides brasiliensis was isolated from a metabolic extract of the fungus. The extraction was made by specific adsorption to and subsequent elution from a column containing a cross linked polymer to which the antibodies of a monospecific rabbit serum had been covalently attached. The purity of the final product was demonstrated by immunodiffusion analysis of the eluate using immune serum produced in a sensitized rabbit. The purified antigen was shown to have cationic electrophoretic mobility and alkaline phosphatase activity.
D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D000946:Antigens, Fungal / Q000302:isolation & purification*; D005658:Fungi / Q000276:immunology*; D007106:Immune Sera; D007122:Immunoelectrophoresis; D010228:Paracoccidioides / Q000201:enzymology / Q000276:immunology*; D011817:Rabbits
D000946:Antigens, Fungal; D007106:Immune Sera; D000469:Alkaline Phosphatase