Thin layer chromatographic estimation of available lysine in dried milk powder.
A rapid method for the estimation of available lysine in dried milk powder has been developed. Samples were treated with 2,4,6-trinitrobenzene sulfonic acid and then hydrolyzed with 6N HCl. The hydrolysate was purified and subjected to ascending thin layer chromatography to isolate xi-trinitophenyl lysine in pure form. The color was read at 346 nm in a specitrophotometer.
D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D008239:Lysine / Q000032:analysis*; D008892:Milk / Q000032:analysis*; D014302:Trinitrobenzenesulfonic Acid
D014302:Trinitrobenzenesulfonic Acid; D008239:Lysine