Blood sampling techniques for studying rapidly turning over metabolic fuels in mice.
Experiments were carried out in control and Ehrlich ascites carcinomatous mice to determine whether orbital venous sinus blood could be used to reflect blood in the systemic circulation (decapitation blood) in the case of a rapidly turning over metabolic fuel such as free fatty acids. The early time course of intravenously injected, labeled free fatty acids was measured using (9, 10-(3)H) palmitic acid and (1-(14)C) linoleate complexed to mouse serum. No significant differences between decapitation and orbital sinus blood were found at early times in either group of mice. The orbital sinus clearly contains blood that is not stagnant and is replaced so rapidly that it is suitable for studying very rapidly turning over, circulating metabolites.
D000818:Animals; D001800:Blood Specimen Collection; D002286:Carcinoma, Ehrlich Tumor / Q000097:blood*; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D007700:Kinetics; D008041:Linoleic Acids / Q000097:blood; D051379:Mice / Q000097:blood*; D010169:Palmitic Acids / Q000097:blood; D013997:Time Factors
D005230:Fatty Acids, Nonesterified; D008041:Linoleic Acids; D010169:Palmitic Acids