Role of cellular density, in vitro, in anti-tumor activity of CFA-treated and immunized cells.
Incorporation of tritiated deoxythymidine (3HdT) into DNA was used to measure growth, in vitro, of P815 tumor cells admixed with spleen and peritoneal effector cells. At a high tumor cell density ((1x10(5) cells per dish), using anti-theta and anti-macrophage sera, T-cells and macrophages from the peritoneum of immunized mice could be identified as cells possessing anti-tumor activity. A nonspecific inhibition by normal effector cells, which occurred at the high tumor cell density, did not occur at a lower tumor cell density (1x10(4) cells per dish). Therefore, the effects of immunization and Freund's adjuvant treatment on the anti-tumor activity of effector cells were determined more accurately when normal cells were no longer inhibitory. Thus, experimental variables dealing with cellular density (cells/mm2 of the culture vessel surface) and effector:tumor cell ratios play an important role in the anti-proliferative capacity of effector cells.
D000818:Animals; D000970:Antineoplastic Agents; D002452:Cell Count; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D005620:Freund's Adjuvant; D006041:Goats / Q000276:immunology; D007114:Immunization; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D013936:Thymidine / Q000378:metabolism
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D005620:Freund's Adjuvant; D013936:Thymidine