Adenosine triphosphate-evoked catecholamine release in chromatin granules. Osmotic lysis as a consequence of proton translocation.
Chromaffin granules suspended in C1-containing media release catecholamine and protein when ATP is added. This phenomenon is inhibited in hyperosmotic media and in the presence of uncouplers of oxidative phosphorylation. Release requires a permeant anion in the medium, but is independent of the cation. The release process appears to be driven by an inwardly directed proton-translocating adenosine triphosphatase. The resulting proton-anion influx causes osmotic lysis of the chromaffin granules.
D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002712:Chlorides; D002838:Chromaffin System / Q000378:metabolism*; D003470:Culture Media; D004140:Dinitrophenols / Q000494:pharmacology; D004837:Epinephrine / Q000378:metabolism; D066298:In Vitro Techniques; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D011506:Proteins / Q000378:metabolism
D002395:Catecholamines; D002712:Chlorides; D003470:Culture Media; D004140:Dinitrophenols; D011506:Proteins; D000255:Adenosine Triphosphate; D004837:Epinephrine