A method of preparation and  application of nitrous acid as a mutagen in Claviceps purpurea.
A simple and speedy procedure is described for the preparation and application of nitrous acid in mutagenic experiments. The conventional incubation mixture of sodium nitrite solution with acetate buffer is replaced by an aqueous solution of nitrous acid prepared by running a solution of sodium nitrite through a column of ion exchange.  The procedure eliminates also the interference of other substances. Experimental appraisal of the technique was done during the mutagenic improval of the fungus Claviceps purpurea.
D002967:Claviceps / Q000187:drug effects*; D008722:Methods; D009153:Mutagens; D009573:Nitrites / Q000494:pharmacology*; D009608:Nitrous Acid / Q000138:chemical synthesis / Q000494:pharmacology*
D009153:Mutagens; D009573:Nitrites; D009608:Nitrous Acid