[Polyuridylic sequences and negative strands of Sindbis virus-specific RNAs: study by affinity chromatography on poly (A)-sepharose columns (author's transl)].
The experimental conditions of affinity chromatography on poly (A)-Sepharose columns have been determined. This method makes obvious the existence of polyuridylic acid sequences on the negative strands of Sinbis virus-spedific RNAs. The isolated RNAs are negative and positive strands hybrids. By polyacrylamide gel electrophoresis, it has been shown that the negative strands have the same length as the 26 S interjacent RNA at the 6th hour, and as the 42 S virion RNA at the 9th hour postinfection. The polyadenylic acid sequences of the virion RNA and of the replicative intermediate are therefore probably genetically coded.
D001483:Base Sequence; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D009693:Nucleic Acid Hybridization; D009694:Nucleic Acid Precursors / Q000096:biosynthesis; D011061:Poly A; D011072:Poly U; D012367:RNA, Viral / Q000302:isolation & purification; D012685:Sepharose; D012845:Sindbis Virus / Q000378:metabolism*; D014779:Virus Replication
D009694:Nucleic Acid Precursors; D012367:RNA, Viral; D011061:Poly A; D011072:Poly U; D012685:Sepharose
Séquences poly(U) et chaĭnes complémentaires des ARN spécifiques du virus Sindbis: étude par chromatographie d'affinité sur poly (A)-sépharose