Glycoproteins of the plasma membrane of Dictyostelium discoideum during development.
Differential binding of 125I-Con A to whole cells throughout the life cycle of dictyostelim discoideum indicates that the appearance of Con A binding proteins on the plasma membrane is under developmental regulation. Con A-Sepharose affinity chromatography of radio-iodinated plasma membrane preparations, followed by analysis with SDS-PAGE, revealed that there are at least 15 Con a binding proteins associated with the surface of 0 hr cells. Between 6 and 18 hr of development, the relative intensity of one of the bands, which corresponds to a protein of molecular weight of 150,000 daltons, increases dramatically.
D001665:Binding Sites; D002462:Cell Membrane / Q000032:analysis* / Q000378:metabolism; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000378:metabolism; D004023:Dictyostelium / Q000254:growth & development* / Q000648:ultrastructure; D006023:Glycoproteins / Q000032:analysis*; D007457:Iodine Radioisotopes; D007784:Lactoperoxidase; D008970:Molecular Weight; D009154:Mutation; D009235:Myxomycetes / Q000254:growth & development*
D006023:Glycoproteins; D007457:Iodine Radioisotopes; D003208:Concanavalin A; D007784:Lactoperoxidase