title: Artefacts due to sodium dodecylsulfate polyacrylamide gel electrophoresis in the study of human erythrocyte membrane calcium binding protein.
abstract: Sodium dodecylsulfate polyacrylamide gel electrophoresis is not usable for identification of calcium binding protein from human erythrocyte membrane; Ca2+ binds to anionic sites of SDS and with 45Ca radioactivity bands appear in gels in absence of proteins.
mesh_terms: D002118:Calcium / Q000097:blood*; D002352:Carrier Proteins / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000032:analysis / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D008565:Membrane Proteins / Q000097:blood*; D012967:Sodium Dodecyl Sulfate
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D012967:Sodium Dodecyl Sulfate; D002118:Calcium