Artefacts due to sodium dodecylsulfate polyacrylamide gel electrophoresis in the study of human erythrocyte membrane calcium binding protein.
Sodium dodecylsulfate polyacrylamide gel electrophoresis is not usable for identification of calcium binding protein from human erythrocyte membrane; Ca2+ binds to anionic sites of SDS and with 45Ca radioactivity bands appear in gels in absence of proteins.
D002118:Calcium / Q000097:blood*; D002352:Carrier Proteins / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000032:analysis / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D008565:Membrane Proteins / Q000097:blood*; D012967:Sodium Dodecyl Sulfate
D002352:Carrier Proteins; D008565:Membrane Proteins; D012967:Sodium Dodecyl Sulfate; D002118:Calcium