title: Agglutination reactions of spontaneous canine tumour cells, induced by concanavalin A, demonstrated by an isotopic assay.
abstract: Quantitative assessment of the agglutination of 51Cr labelled canine cell suspensions to canine kidney cell monolayers has been performed over a range of concanavalin A concentrations. Agglutination was observed with all cell cultures tested, comprising four spontaneous canine melanomas, two canine mammary carcinomas, a benign mammary tumour and a contact-inhibited kidney cell line. The melanomas tested showed strong specific inhibition of concanavalin A agglutination by 10(-2)M alpha-methyl-D-glucopyranoside. Inhibition of agglutination of mammary tumour and kidney cells was weaker and less specific. Agglutination was inhibited at 4degrees C. Reduced agglutination to glutaraldehyde-fixed mono-layers was observed in the case of mammary tumours but was absent when contact-inhibited kidney cells were tested. The specificity of the reaction for transformed cells and the parameters involved are discussed.
mesh_terms: D000371:Agglutination / Q000187:drug effects*; D000372:Agglutination Tests / Q000379:methods; D000818:Animals; D002460:Cell Line; D002860:Chromium Radioisotopes; D003208:Concanavalin A / Q000008:administration & dosage / Q000494:pharmacology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D008325:Mammary Neoplasms, Experimental / Q000276:immunology*; D008545:Melanoma / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology
chemical_list: D002860:Chromium Radioisotopes; D003208:Concanavalin A; D014357:Trypsin