[Reparation of viability of arthrospores subjected to boiling in Actinomyces streptomycini].
Endospores of Streptomyces streptomycini B-16 are considerably more thermostable (by 40degreesC) than it was generally accepted. The bulk of populations of the spores (up to 55%) can be subjected to 100degreesC during 10 min but does not germinate under usual conditions as a result of heat damage which may be repaired at a relative humidity of 100% or in water suspensions at temperatures optimal for growth of the actinomycete. The rate of reparation increase upon washing of the heated spores with stirring, centrifuging and especially after treating the spores with ultrasound. The treatment is supposed to eliminate from the spores growth inhibitors which were formed as a result of heat damage. Characteristics of the process are discussed, which make it different from other reparation systems described elsewhere, in particular its endogenous character.
D000190:Actinomyces / Q000254:growth & development*; D006358:Hot Temperature; D006813:Humidity; D013171:Spores, Bacterial / Q000254:growth & development; D014465:Ultrasonics
Vosstanovlenie zhiznesposobnosti artrospor Actinomyces streptomycini, podvergnutykh kipiacheniiu