title: Staining of the spindle apparatus in human lymphocyte and fibroblast cultures.
abstract: The spindle apparatus of human mitoses can be preferentially stained with Giemsa if the cells are fixed with methanol--acetic acid and treated for about 15 min with 0.1 X SSC at 92 degrees C and for 18 h with 2 X SSC at 60 degrees C.
mesh_terms: D001399:Azure Stains; D002478:Cells, Cultured; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008938:Mitosis; D009940:Organoids
chemical_list: D001399:Azure Stains