Use of hydroxyapatite-coasted glass beads for preclinical testing of potential antiplaque agents.
Methods for rapid preclinical testing of antiplaque agents in vitro using hydroxyapatite (HT)-coated glass beads are described. The assays developed could reliably detect (i) prevention of growth in the culture fluid or on the HT surfaces, (ii) the effect of transient exposure of a bactericidal agent on the viability of cells in a preformed bacterial mat, (iii) reversible adsorption of a bactericidal agent on an HT surface, and (iv) the ability of an agent to inhibit adsorption of Streptococcus sanguis to an HT surface or to salivary proteins adsorbed to an HT surface.
D000327:Adsorption; D002327:Cariostatic Agents / Q000494:pharmacology*; D003773:Dental Plaque / Q000517:prevention & control*; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D005459:Fluorides / Q000494:pharmacology; D005898:Glass; D006801:Humans; D006882:Hydroxyapatites; D010919:Placebos; D012471:Salivary Proteins and Peptides / Q000378:metabolism; D013295:Streptococcus mutans / Q000187:drug effects; D013298:Streptococcus sanguis / Q000187:drug effects; D014678:Veillonella / Q000187:drug effects
D002327:Cariostatic Agents; D006882:Hydroxyapatites; D010919:Placebos; D012471:Salivary Proteins and Peptides; D005459:Fluorides