Detection and estimation of azomethane in expired air of 1,2-dimethylhydrazine-treated rats.
Azomethane (AM) gas was identified as a major metabolite of 1,2-dimethylhydrazine (1,2-DMH) in the expired air of F344 rats. The compound was characterized by high-pressure liquid chromatography, gas chromatography, and mass spectrometry, in comparison to a synthetic standard. At a dose of 21 mg 1,2-DMH/kg sc, approximately 14 and 11% of the dose were exhaled as AM and CO2, respectively, in 24 hours. At 200 mg 1,2-DMH/kg, 23 and 4% of the dose appeared as AM and CO2, respectively, in the respired air within the same period. Most AM was seen in the first 6 hours, but the CO2 evolution was more progressive, especially after the higher dose of 1,2-DMH.
D000818:Animals; D001391:Azo Compounds / Q000032:analysis / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D003110:Colonic Neoplasms / Q000139:chemically induced; D004127:Dimethylhydrazines / Q000378:metabolism*; D006834:Hydrazines / Q000378:metabolism*; D008297:Male; D008697:Methane / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012119:Respiration; D013997:Time Factors
D001391:Azo Compounds; D004127:Dimethylhydrazines; D006834:Hydrazines; D002245:Carbon Dioxide; C001825:azomethane; D008697:Methane