A simple and sensitive fluorometric assay method for taurine using high-voltage paper electrophoresis.
A simple and sensitive fluorometric assay method for taurine (2-aminoethanesulfonic acid) has been developed. For the separation of taurine, high voltage paper electrophoresis subsequent to column chromatographic procedures was employed. Fluorescent product of taurine was yielded by spraying fluorescamine (4-phenylspiro [furan-2(3H), 1'-phthalan]-3, 3'-dione) and borate buffer on the paper, and the fluorescence was assayed spectro-fluorometrically after eluting with 50% ethanol. The linear relationship between the concentration of taurine and fluorescence developed was achieved over the concentration ranges of 0.5-10 nmoles, and the recoveries obtained were 90-100%. The specificity of this method for taurine was satisfactory and structural analogues involved in the metabolic pathway of taurine did not interfere with the assay. Examples for tissue levels of taurine in various organs of the rat as determined by this new method are also presented.
D000818:Animals; D002845:Chromatography; D004590:Electrophoresis, Paper / Q000379:methods; D005450:Fluorescamine; D005470:Fluorometry; D008297:Male; D008722:Methods; D051381:Rats; D013654:Taurine / Q000032:analysis*
D013654:Taurine; D005450:Fluorescamine