A turbidimetric assay method for studying antibody-induced lysis of hapten-coated erythrocytes.
A method is described, based on the application of trubidimetry for study of antibody-mediated complement-dependent lysis of hapten-coated erythrocytes. The procedure is simple, reliable and sensitive. Time-rate studies over the entire range of interaction between the reactants are possible, with a minimal consumption of materials and time. The method also gives the possibility of adding substances to the reaction mixture, even at high concentrations, without introducing errors by multiple handling and washing.
D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D003165:Complement System Proteins; D004912:Erythrocytes / Q000276:immunology*; D006241:Haptens; D006461:Hemolysis; D009391:Nephelometry and Turbidimetry; D010783:Photometry; D011817:Rabbits
D000906:Antibodies; D006241:Haptens; D003165:Complement System Proteins