Effect of sodium fluoride on glycolysis in human erythrocytes and Ehrlich ascites tumour cells in vitro.
In human erythrocytes and Ehrlich ascites tumour cells, NaF inhibites aerobic glucose utilization and lactate formation. The inhibition of glycolysis was accompanied by a decrease in cellular pyruvate and ATP, and by accumulation of 2-hosphoenolpyruvate. These results and direct enzymatic determinations showed that fluoride inhibits, in addition to enolase (phosphopyruvate hydratase, EC 4.2.1.11), also pyruvate kinase.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002460:Cell Line; D004912:Erythrocytes / Q000378:metabolism*; D005459:Fluorides / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects*; D006801:Humans; D010751:Phosphopyruvate Hydratase / Q000037:antagonists & inhibitors; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors; D012969:Sodium Fluoride / Q000494:pharmacology*
D012969:Sodium Fluoride; D011770:Pyruvate Kinase; D010751:Phosphopyruvate Hydratase; D005459:Fluorides