[Diminution of activity of liver microsomal oxygenase in a rat fed with a diet with added zineb].
Young Wistar CF male rats were fed with diets containing 6,15,60,600,3600 p.p.m of zineb (a zinc thiocarbamate used as fongicide). Four weeks after the beginning of the treatment, a calcic precipitation method is used for obtaining a preparation of liver microsomes from these animals. The microsomes are then used for measuring the activity of both aminophenazone demethylase and aniline hydroxylase as well as the liver concentration of cyt. P 450 and cyt. b5. The weight growth was reduced by the highest dose of zineb, which corresponds to about a daily intake of twentieth of the LD 50 per os. The fongicide brings down the activity of the microsomal monoxygenases and reduces the P 450 quantity. The lessening of the aminophenazone demethylation is apparent starting from a dose between 60 and 600 p.p.m...
D000632:Aminopyrine / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D004032:Diet; D006900:Hydroxylation; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010105:Oxygenases / Q000378:metabolism*; D051381:Rats; D013859:Thiocarbamates / Q000633:toxicity*; D015038:Zineb / Q000633:toxicity*
D000814:Aniline Compounds; D013859:Thiocarbamates; D000632:Aminopyrine; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D015038:Zineb
Diminution d'activité des oxygénases microsomales du foie chez le rat nourri avec un régime additionné de zinébe