[The change of template activity of dog kidney chromtin by polyene antibiotics in vivo and in vitro].
Effect of amphotericin B and nistatin on template activity of nuclear membrane-bound (DNPm) and free (DNPo) dog kidney chromatin after intravenous injections of antibiotics and after the incubation of isolated kidney cell nuclei with the antibiotics is studied. It is found that injections of amphotericin B and nistatin resulted in the increase of DNPo template activity in RNA polymerase system, the stimulating effect of nistatin being higher than that of amphotericin B. Injections of nistatine stimulated also template activity of DNPm, while amphotericin B produced no effect on DNPm. When studing the effect of polyene antibiotics on template activity of DNPo and DNPm in vitro, it is found that the intensity of RNA synthesis after incubation of isolated nuclei with antibiotics is considerably increased, and stimulating effect of nistatin is higher than of amphotericin B. Both antibiotics produced no effect on template activity of DNP in vitro. Thus, comparative analysis of changes in template activity of dog kidney chromatin under the effect of polyene antibiotics in vivo and in vitro has revealed the similarity of these drugs and draws to the conclusion that nistatin and amphotericin B produce a direct effect on template activity of chromatin.
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004285:Dogs; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008566:Membranes; D009761:Nystatin / Q000494:pharmacology*; D011485:Protein Binding; D013698:Templates, Genetic
D002843:Chromatin; D009761:Nystatin; D000666:Amphotericin B; D012321:DNA-Directed RNA Polymerases
Izmenenie matrichnoĭ aktivnosti khromatina pochek sovak pod vliianiem polienovykh antibnotikov in vivo i in vitro