High performance liquid chromatography of natural products. I. Separation of cephalosporin C derivatives and cephalosporin antibiotics;isolation of cephalosporin C from fermentation broth.
Microbonded propylamine silica with a solvent system containing acetic acid, methanol, acetonitrile and water (2:4:7.5:86.5) is suitable for an efficient separation of mixtures containing several closely related cephem derivatives. The same system with preparative columns was used for the isolation of cephalosporin C directly from the filtered broth of C. acremonium fermentation.
D000164:Acremonium / Q000378:metabolism; D002511:Cephalosporins / Q000096:biosynthesis / Q000302:isolation & purification*; D005285:Fermentation; D008722:Methods; D012997:Solvents
D002511:Cephalosporins; D012997:Solvents