Differential inhibition of trophoblast outgrowth and inner cell mass growth by actinomycin D in cultured mouse embryos.
Treatment of preimplantation mouse embryos in vitro with 10(-3) to 10(-1) mug actinomycin D/ml for 2 hr showed that (i) postimplantation development in vitro was inhibited most when embryos were treated at the morula stage and (ii) after the morula stage actinomycin D inhibited trophoblast outgrowth less than inner cell mass development.
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D003609:Dactinomycin / Q000008:administration & dosage / Q000494:pharmacology*; D004622:Embryo, Mammalian; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D013480:Superovulation; D014327:Trophoblasts / Q000187:drug effects*
D003609:Dactinomycin