[Determination of Stokes' radii of the cytoplasmic receptor molecules for estradiol in various organs of rats].
A method of gel-filtration of Sepharose 6B was used; Stoke's radii (a) of some forms of cytoplasmic receptors of estrogens of the uterus, kidneys, and the liver of male and female rats were determined. In the initial cytozol in elution with buffer of low ionic strength estrogenic uterine receptors were presented chiefly by the form with a congruent to 70A, and the receptors of the male and female kidneys--by the form with a congruent to 30 A; receptors of the liver of males and females were represented by both forms, in females the content of the form with a congruent to 70 A somewhat exceeded the content of the form with a congruent to 30 A. At the same time in the males the form with a congruent to 30 A prevailed. In elution with a buffer of a high ionic strength the receptors of all the organs under study eluated with a congruent to 20--26 A. Formation of a form with a congruent 20--26 A in case of receptors of the liver of males and the uterus was reversible under the action of a high salt concentration. Receptors of all the organs under study were capable of forming Ca2+-stabilized form with a congruent to 35 A in elution with a buffer of low ionic power and a congruent to 21-26 A in elution with a buffer of high ionic strength.
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D003593:Cytoplasm / Q000032:analysis; D004958:Estradiol; D005260:Female; D007668:Kidney / Q000166:cytology; D008099:Liver / Q000166:cytology; D008297:Male; D051381:Rats; D011960:Receptors, Estrogen; D014599:Uterus / Q000166:cytology
D011960:Receptors, Estrogen; D004958:Estradiol
Opredelenie radiusov Stoksa tsitoplazmaticheskikh retseptornykh molekul dlia estradiola v nekotorykh organakh krys