Study of a microassay for the quantitation of cytotoxic antitumor antibody, using 125I-iododeoxyuridine as a target cell label; detection of a static effect on cell DNA synthesis.
The different steps of a microassay for the quantitation of cytotoxic antitumor antibody, are studied in detail. It is shown that the use of 125I-Iododeoxyuridine as the target cell label, at the end of the assay, after action of serum and complement, allows the advantages available with this isotopic label to be retained and to avoid the main disadvantage due to its non specific toxicity for the target cells. Furthermore, this technique is shown to be able to detect non only a killer effect of immune sera on specific target cells with complement but also an additionnal static effect of DNA synthesis : this effect is complement dependent and transitory.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis*; D002470:Cell Survival / Q000187:drug effects; D046508:Culture Techniques; D003601:Cytotoxicity Tests, Immunologic; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D005260:Female; D007065:Idoxuridine / Q000494:pharmacology; D008748:Methylcholanthrene; D051379:Mice; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology; D012509:Sarcoma / Q000139:chemically induced / Q000276:immunology
D000912:Antibodies, Neoplasm; D004273:DNA, Neoplasm; D008748:Methylcholanthrene; D007065:Idoxuridine