Comparative studies on platelet membrane preparations.
For the isolation of platelet plasma and cytoplasmatic membranes, the following methods were applied. 1. Glycerol-osmotic lysis technique, combined with sonication. 2. Glycerol-osmotic lysis technique carried out on fresh or frozen platelets. 3. Detergent treatment followed by separation of the protein fractions insoluble at low ionic strength. The detergents used were Triton X-100, Nonidet P-40, Na-deoxycholate and digitonin. The protein fractions were compared by SDS-PAG electrophoresis.
D001792:Blood Platelets / Q000166:cytology*; D001798:Blood Proteins; D002462:Cell Membrane; D002499:Centrifugation, Density Gradient; D003902:Detergents; D005990:Glycerol; D008970:Molecular Weight; D009997:Osmotic Pressure; D012995:Solubility
D001798:Blood Proteins; D003902:Detergents; D005990:Glycerol