[Intermediates of erythrocyte glycolysis during three days hypercapnia in the dog (author's transl)].
Ten mongrel dogs (mean weight: 27 kg) awake and with an implanted femoral catheter have been maintained for three days in a controlled chamber (10% CO2 and 21% O2). Arterial blood samples, taken before admission and after one, two, four, six, 24, 48 and 72 hours of exposure, allowed to study blood gases and acid-base equilibrium. Glycemia, phosphatemia, erythrocyte concentration of glucose-6-phosphate (G-6-P), fructose-6-phosphate (F-6-P), fructose-1,6-diphosphate (F-1,6-DP), 2,3-diphosphoglycerate (2,3-DPG), pyruvate, lactate and ATP were also titrated by various enzymatic methods. In addition, nine reference subjects were studied in air (without CO2). During the hypercapnia, [H+] rapidly increases to 70 nmol/1, then progressively decreases after 24 hours, while [HCO3-] slowly rises. The glycemia stays high during the whole exposure. There is also an increase in inorganic phosphate, G-6-P and F-6-P, but during the first 24 hours only. F-1,6-DP, pyruvate and lactate remain lowered during the whole exposure. The 2,3-DPG diminishes after the sixth hour. These phenomena, related to the acidosis and probably to the phosphofructokinase inhibition don't arise in the reference subjects. However the latter present after a two and four hour-stay in the chamber a small decrease in pyruvicemia and lactacidemia.
D000818:Animals; D001639:Bicarbonates / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D002245:Carbon Dioxide / Q000097:blood; D004163:Diphosphoglyceric Acids / Q000097:blood; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism*; D005636:Fructosephosphates / Q000097:blood; D005958:Glucosephosphates / Q000097:blood; D006019:Glycolysis; D006935:Hypercapnia / Q000097:blood*; D007773:Lactates / Q000097:blood; D010100:Oxygen / Q000097:blood; D011773:Pyruvates / Q000097:blood
D001639:Bicarbonates; D001786:Blood Glucose; D004163:Diphosphoglyceric Acids; D005636:Fructosephosphates; D005958:Glucosephosphates; D007773:Lactates; D011773:Pyruvates; D002245:Carbon Dioxide; D010100:Oxygen
Intermédiaires de la glycolyse érythrocytaire au cours d'une hypercapnie de trois jours chez le chien