[Isolation and purification of NADP-dependent "L-malate"-enzyme from corn leaves].
Isolation and purification of "malic-enzyme" NADP was done using fractionation by ammonium sulfate, anion-exchange chromatography on DEAE cellulose, gel-filtration through Sephadex G-200 and purification on DEAE Sephadex A-50. The isoenzyme isolated had a specific activity of 40-50 mkM/mg protein per min (approximately 80-fold purification) and contained negligible admixtures.
D000645:Ammonium Sulfate; D005591:Chemical Fractionation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000302:isolation & purification*; D008291:Malate Dehydrogenase / Q000302:isolation & purification*; D003313:Zea mays
D007527:Isoenzymes; D008291:Malate Dehydrogenase; D000645:Ammonium Sulfate
Vydelenie i ochistka NADP-zavisimogo "malik"-enzima iz list'ev kukuruzy