[Purification of pancreatic kallikrein by the method of affinity chromatography].
Pancreatic Kallikrein was purified by affinity chromatography on BPTI-Sepharose. Immobilized BPTI was prepared via three stages: a) formation of the BPTI--acetylated trypsin complex; b) coupling of the resultant complex with CNBr-activated Sepharose; c) dissociation of the bound complex at pH 2.0 to yield immobilized BPTI and acetylated trypsin. The final product contained 59 X 10(-3) micronmoles insolubilized BPTI/ml Sepharose. Trypsin occurring in commercial Kallikrein preparations was separated by the batch procedure with ovomucoid-Sepharose. This method allows 100-fold purification of commercial Kallikrein.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D007525:Isoelectric Focusing; D007610:Kallikreins / Q000032:analysis / Q000302:isolation & purification*; D010059:Ovomucin / Q000302:isolation & purification / Q000494:pharmacology; D010179:Pancreas / Q000201:enzymology*; D014357:Trypsin / Q000032:analysis
D010059:Ovomucin; D007610:Kallikreins; D014357:Trypsin
Ochistka pankreaticheskogo kallikreina metodom afinnoĭ khromatografii