title: Thermal compensation in protein and RNA synthesis during the intermolt cycle of the American lobster, Homarus americanus.
abstract: 1. The in vitro rates of incorporation of precursors into protein and RNA and the concentration of RNA were measured in tissues of intermolt and premolt lobsters acclimated to 5 degrees C and 20 degrees C. Midgut gland, abdominal muscle and gill of intermolt lobsters respond to temperature acclimation by a compensatory translation of the rate-temperature (R-T) curves with respect to the rates of incorporation of 3H-leucine and 3H-uridine into the acid-insoluble fraction. Midgut gland and muscle of premolt animals exhibit either no compensation or inverse compensation; gill tissue exhibits a rotation of the R-T curve. 2. The existence of the complete de novo pathway of pyrimidine biosynthesis is demonstrated in the class Crustacea. NaH14 CO2 is incorporated into orotic acid and orotic-14 C-acid is incorporated into the acid-insoluble fraction. 3. Both the concentration of RNA and the rates of incorporation of precursors of both the salvage and de novo pyrimidine pathways are enhanced in the midgut gland of premolt lobsters, relative to intermolt tissue, under conditions of warm-acclimation.
mesh_terms: D000064:Acclimatization; D000818:Animals; D005880:Gills / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000033:anatomy & histology; D009132:Muscles / Q000378:metabolism; D008121:Nephropidae / Q000378:metabolism / Q000502:physiology*; D014176:Protein Biosynthesis; D011743:Pyrimidines / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis*; D013696:Temperature
chemical_list: D011743:Pyrimidines; D012313:RNA