Sulfate incorporation from ascorbate 2-sulfate into chondroitin sulfate by embryonic chick cartilage epiphyses.
Radioactivity was significantly incorporated from ascorbate 2-[35S]sulfate into chondroitin sulfate by embryonic chick cartilage epiphyses. The extent of incorporation was comparable with that from inorganic [35S]sulfate. The radioactive chondroitin sulfate formed from ascorbate 2-[35S]sulfate gave two radioactive disaccharides on chondroitinase-ABC [EC 4.2.2.4] digestion. The incorporation was markedly decreased by inorganic sulfate. The time course of incorporation from ascorbate 2-[35S]sulfate and inorganic [35S]sulfate into chondroitin sulfate and the constituent disaccharides suggest that the incorporation rates from the two radioactive substances are different.
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D002642:Chick Embryo; D002807:Chondroitin / Q000031:analogs & derivatives*; D002808:Chondroitin Lyases / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000378:metabolism*; D004187:Disaccharides; D004838:Epiphyses / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*
D004187:Disaccharides; D013431:Sulfates; D002807:Chondroitin; D002809:Chondroitin Sulfates; D002808:Chondroitin Lyases; D001205:Ascorbic Acid