Echinococcus granulosus: development in vitro from oncosphere to immature hydatid cyst.
Echinococcus granulosus cysts were cultured in vitro from the oncosphere, and cyst morphogenesis is described. Cysts grew to a mean diameter of 16 mm in 120 days, with a maximum of 20 mm. An amorphous layer was apparent around the larvae during early post-oncospheral re-organization. Organisms became vesicular at 6 days and laminations appeared in the amorphous layer at 10 days. Confluence of proliferating cells to form the syncytium of the germinal membrane was observed.
D000818:Animals; D003470:Culture Media; D004285:Dogs; D004446:Echinococcus / Q000166:cytology / Q000254:growth & development*; D009024:Morphogenesis
D003470:Culture Media