[Relationship between the structural state of chromatin and the biosynthesis of rapidly labeling nuclear RNA in the thymuses of rats subjected to total x-irradiation].
Accumulation of polydeoxyribonucleotides (PDN) was studied in thymus of rats, subjected to total body X-ray irradiation at doses 400 and 800 rad. The content of PDN correlated with the time of treatment; its low-polymer components prevailed after irradiation with 800 rad. Protein/DNA ratio was decreased in DNP within 30 min after the irradiation and it returned to the initial level within 4 hrs. Capacity of DNP to make a complex with actinomycin D was inhibited within 30 min; however it exceeded the control level within 4 hrs after irradiation. Biosynthesis of nuclear RNA, estimated by incorporation of 14C-uridine, was distinctly decreased within the whole experimental period.
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D003609:Dactinomycin / Q000378:metabolism; D003852:Deoxyribonucleoproteins / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000378:metabolism; D011485:Protein Binding; D012313:RNA / Q000096:biosynthesis*; D011833:Radiation Injuries, Experimental / Q000378:metabolism*; D051381:Rats; D013950:Thymus Gland / Q000378:metabolism*; D014965:X-Rays
D002843:Chromatin; D003852:Deoxyribonucleoproteins; D009698:Nucleoproteins; D011089:Polydeoxyribonucleotides; D003609:Dactinomycin; D012313:RNA
Sootnoshenie strukturnogo sostoianiia khromatina i biosinteza bystrometiashchikhsia iadernykh RNK v timuse krys, podvergnutykh obshchemu rentgenovskomu oblucheniiu