Immune response in the garter snake (Thamnophis ordinoides).
Garter snakes (Thamnophis ordinoides) were immunized with hen egg albumin, human gamma-globulin and Keyhole limpet haemocyanin in Freund's adjuvant. Antibody was consistently detected by radioimmunoelectrophoresis and in three different gamma- and beta-globulin precipitin lines called IgM (approximately or equal to 20S), Ig-1 (approximately or equal to 9S) and Ig-2 (approximately or equal to 8-5S). Early antibody (day 31 after immunization) was frequently Ig-M whereas Ig-2 and especially Ig-1 were detectable for the longest duration (992 days). After immunization with antigen in Freund's adjuvant, Ig-1 serum concentration showed the greatest increase, from almost undetectable levels to the most prominent immunoglobulin in immune serum.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M; D007136:Immunoglobulins / Q000032:analysis; D012911:Snakes / Q000276:immunology*; D013997:Time Factors
D000906:Antibodies; D007075:Immunoglobulin M; D007136:Immunoglobulins