[Electrofocusing of immunoglobulin G that reacts positively in the sedimentation reaction for cancer].
Immunoglobulin G from the serum of patients with myeloma and positively reacting in the sedimentary test for cancer (PPR-STC) was purified by DEAE-Spehadex A-50 and KM-cellulose chromotography and studied by the method of isoelectrofocusing; Application of 1% ampholine within the pH gradient 3.0-10.0 shows the difference between the isoelectric spectra of immunoglobulin G from the donor and from a patient with myeloma. The PPR-STC isoelectric point located in the zone of pH 8.4 is determined. No subfraction with pH 8.4 is observed in the fraction of the donor immunoglobulin G.
D001799:Blood Sedimentation; D002848:Chromatography, DEAE-Cellulose; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis*; D007525:Isoelectric Focusing; D009101:Multiple Myeloma / Q000276:immunology
D007074:Immunoglobulin G
Elektrofokusuvannia imunohlobulinu G, shcho pozytyvno reahuie v osadoviĭ reaktsiï na rak