[Biological methylations in the crab Carcinus maenas; in vitro inhibition by a fraction purified from androgen gland extracts].
Extracts from muscles, testis, seminal vesicles and ovaries of the Crab, Carcinus maenas, have been studied in vitro, in presence of [14C]-methyl S-adenosylmethionine, with an E. coli tRNA as methyl acceptor. The highest level of methylases is found in the testis. It has been reported previously that a purified fraction extracted from the androgenic glands of Carcinus maenas inhibits the vitellogenesis in ovaries. We now show that the same fraction inhibits tRNA methylation in an extract of testis as methylase; a 50% inhibition is obtained with about 10 mug of a purified fraction corresponding to 15 glands. With an enzymatic preparation from the ovaries, a 50% inhibition of the tRNA methylase is observed with the purified extract from 4 glands.
D000818:Animals; D003386:Brachyura / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008297:Male; D009132:Muscles / Q000378:metabolism; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism*; D011506:Proteins / Q000502:physiology*; D012669:Seminal Vesicles / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*; D014020:Tissue Extracts; D012359:tRNA Methyltransferases / Q000378:metabolism*
D011506:Proteins; D014020:Tissue Extracts; D012359:tRNA Methyltransferases
Méthylations biologiques chez le crabe Carcinus maenas; inhibition in vitro par une fraction purifiée extraite des glandes androgènes