title: Genetic analysis of radiation-sensitive mutations in the slime mould Dictyostelium discoideum.
abstract: The linkage of two mutations leading to increased sensitivity to ultraviolet light and 60Co gamma rays was determined in the slime mould Dictyostelium discoideum using a genetic analysis based on the parasexual cycle. Diploids were selected from a mixture of radiation-sensitive, temperature-resistant and radiation-resistant, temperature-sensitive haploids on the basis of simultaneous radiation and temperature resistance. Analysis of drug-resistant haploid segregants of the heterozygous diploids indicated that one of the radiation-sensitive mutations, radA20, was linked to linkage group I whereas the other, radB13, was linked to the recently defined linkage group VI.
mesh_terms: D003037:Cobalt Radioisotopes; D003433:Crosses, Genetic; D003513:Cycloheximide / Q000494:pharmacology; D004023:Dictyostelium / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects*; D004171:Diploidy; D004352:Drug Resistance, Microbial; D008040:Genetic Linkage; D006238:Haploidy; D000432:Methanol / Q000494:pharmacology; D009154:Mutation; D009235:Myxomycetes / Q000528:radiation effects*; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D003037:Cobalt Radioisotopes; D003513:Cycloheximide; D000432:Methanol