title: Binding of methyltrienolone to various androgen-dependent and androgen-responsive tissues in four animal species.
abstract: Comparative sucrose gradient studies of the in vitro binding of dihydrotestosterone (DHT) and of a synthetic androgen, methyltrienolone (R 1881), have been done with the cytosols of various tissues of the rat, mouse, cock and man. With rat prostate cytosol, the amount of R 1881 and DHT binding in the 8-9S region of the gradient was found to be comparable. Specific 8-9S peaks of R 1881 were also found in rat levator ani/bulbocavernosus and skeletal muscles and in the mouse kidney. Only 4-5S peaks could be demonstrated in the cock's comb while DHT under the same conditions showed both 8-9S and 4-5S binding. Binding of R 1881 to the cytosol of the hyperplastic prostate was polydispersed, and showed evidence of the presence of aggregates. Evidence was also found that R 1881 could bind to the progesterone receptor in rat uterus. Our study supports the theory that in a given species the androgen receptors are similar if not identical in all the tissues. The synthetic androgen R 1881 appears to be a useful tool for androgen receptor studies in various animal species provided that the tissue under study contains no progesterone receptor.
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002369:Castration; D002645:Chickens; D003129:Comb and Wattles / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism; D004963:Estrenes / Q000378:metabolism*; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051379:Mice; D009132:Muscles / Q000378:metabolism*; D009928:Organ Specificity; D011467:Prostate / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011944:Receptors, Androgen / Q000302:isolation & purification / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D013045:Species Specificity; D013739:Testosterone / Q000378:metabolism; D045165:Testosterone Congeners / Q000378:metabolism*
chemical_list: D004963:Estrenes; D011944:Receptors, Androgen; D011987:Receptors, Steroid; D045165:Testosterone Congeners; D013196:Dihydrotestosterone; D013739:Testosterone