Comparison of the manifestation of radiation-induced lethal damage in in vitro and in vivo systems.
The kinetics of radiation-induced cell death was compared in in vitro and in vivo systems, using mouse mammary carcinoma cell line, FM3A. Cultured and ascites cells were irradiated with 5 krad of X-rays, and changes in the number of viable cells and in the cell volume after irradiation were analyzed. The volume of irradiated cells in both systems increased about 5 times that of control after 3 generations and was saturated. Cells in vitro began to die after 3 generations. The number of cells in vivo began to decrease immediately after irradiation, but the rate of the decrease became large at 3rd generation. It was concluded that radiation-induced lethal damage manifests itself in the same way at the intracellular level in both systems, and some dying cells in vivo were eliminated before manifestation of lethal damage.
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects*; D051379:Mice; D008938:Mitosis / Q000528:radiation effects; D011829:Radiation Dosage; D014965:X-Rays