The uptake and metabolism of uridine by the slime mould Physarum polycephalum.
1. Uridine is taken up by microplasmodia of Physarum polycephalum via a saturatable transport system with an apparent Km of 29 muM. An intracellular concentration significantly higher than that in the growth medium is attained, suggesting that the uptake is an active process. Both deoxyribonucleosides and ribonucleosides are competitive inhibitors of the uptake of uridine. 2. In contrast, the rate of entry of uridine into surface plasmodia is a linear function of the concentration of the nucleoside in the growth medium, and the uptake is not inhibited by other nucleosides. 3. As well as serving as a source of pyrimidine nucleotides for the synthesis of nucleic acids, uridine is also catabolised by P. polycephalum. Uracil accumulates in the growth medium and there is also significant conversion of C-2 of the pyrimidine ring to CO2. The proportion of uridine subject to catabolism in surface plasmodia is less than that observed for microplasmodia.
D001667:Binding, Competitive; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003562:Cytidine / Q000494:pharmacology; D007700:Kinetics; D009235:Myxomycetes / Q000378:metabolism*; D010804:Physarum / Q000187:drug effects / Q000378:metabolism*; D013936:Thymidine / Q000494:pharmacology; D014498:Uracil / Q000378:metabolism; D014500:Uracil Nucleotides / Q000378:metabolism; D014529:Uridine / Q000378:metabolism*
D014500:Uracil Nucleotides; D014498:Uracil; D003562:Cytidine; D013936:Thymidine; D014529:Uridine