Chemical structure of the peptidoglycan of Vibrio parahaemolyticus A55 with special reference to the extent of interpeptide cross-linking.
The chemical structure of the cell wall peptidoglycan of Vibrio parahaemolyticus A55 was studied. Estimation of cross linkages between peptide subunits in the peptidoglycan by dinitrophenylation showed that about 30% of the total 2,6-diaminopimelic acid (A2pm) residues were involved in cross linkages. The presence of interpeptide bridges was also demonstrated by isolating bisdisaccharide peptide subunit dimers from Chalaropsis muramidase digests of the cell wall peptidoglycan by gel filtration followed by ion-exchange column chromatography, although most of the building blocks obtained were uncross-linked disaccharide peptide monomers. The chain length of a glycan moiety of the peptidoglycan obtained by treatment with the L-11 enzyme and gel filtration of the digest was also studied. The chain length varied from 7 to 44, but 30% of the glycan fragments had muramic acid at the reducing end and a chain length of 28 to 44. In conformity with the above structural study it was demonstrated that a particulate enzyme fraction obtained by differential centrifugation of a sonicated preparation of V. parahaemolyticus catalyzed a penicillin-sensitive transpeptidation reaction, using UDP-MurNAc-14C-pentapeptide and UDP-GlcNAc as substrates.
D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006020:Glycopeptides / Q000378:metabolism; D009112:Muramic Acids / Q000032:analysis; D010400:Penicillin G / Q000494:pharmacology; D010455:Peptides / Q000032:analysis; D010457:Peptidoglycan / Q000032:analysis* / Q000096:biosynthesis; D011487:Protein Conformation; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism; D014538:Uridine Diphosphate N-Acetylmuramic Acid / Q000378:metabolism; D014736:Vibrio parahaemolyticus / Q000032:analysis* / Q000378:metabolism
D000596:Amino Acids; D000606:Amino Sugars; D006020:Glycopeptides; D009112:Muramic Acids; D010455:Peptides; D010457:Peptidoglycan; D014538:Uridine Diphosphate N-Acetylmuramic Acid; D014537:Uridine Diphosphate N-Acetylglucosamine; D005944:Glucosamine; D010400:Penicillin G