[Study of interaction between contrast media and human serum albumin by the fluorescence method].
Structural changes in the human serumal albumin under the effect of roentgen-contrast media, viz. bilignost, triiotrast, endografine, iodamide, triombrine and cardiotrast, were investigated by the method of fluorescence. The complex-formation of albumin with triiodotrast, bilignost and endografine was shown to call forth a short-wave shift of the fluorescence peak by approximately 20 nm. The binding section for these substances is characterized by an association constant equalling 1.2-10(5) M-1. The authors presume that triiodotrast, bilignost and endografine cause changes in the microstructure of the aqueo-protein layer in the area of the tryptophan residue localization. As to triombine, iodamide and cardiotrast fluorescence failed to disclose their combination with the human serumal albumin.
D000100:Acetrizoic Acid / Q000494:pharmacology; D003287:Contrast Media / Q000494:pharmacology*; D003973:Diatrizoate / Q000494:pharmacology; D004347:Drug Interactions; D006801:Humans; D007451:Iodamide / Q000494:pharmacology; D007458:Iodipamide / Q000494:pharmacology; D007468:Iodopyracet / Q000494:pharmacology; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D003287:Contrast Media; D012709:Serum Albumin; D003973:Diatrizoate; D000100:Acetrizoic Acid; D007451:Iodamide; D007458:Iodipamide; D007468:Iodopyracet
Issledovanie vzaimodeĭstviia rentgenokontrastnykh vesh chestv s syvorotochnym al'buminom cheloveka metodom fliuorestsentsii.