title: Purification of an exo-beta-D-glucanase from cell-free extracts of Candida utilis.
abstract: beta-Glucanase present in cell-free extracts from Candida utilis was isolated and purified 562-fold by procedures that include adsorption on DEAE-Sephadex A-50 and filtration through columns of Sephadex G-50, G-100 and G-200, Bio-Gel P-10, and Concanavalin A-Sepharose 4B. The purified enzyme appeared homogeneous on polyacrylamide-gel electrophoresis and in ultracentrifugation studies (S20,w = 1.74S). The enzyme behaved as an acidic glycoprotein (pI4.1) with 68% carbohydrate and a high content of acidic amino acids. The mol.wt. was estimated to be 20000 from gel filtration and polyacrylamide-gel electrophoresis and 36000 from sedimentation experiments. Studies on the hydrolysis of different substrates showed that the enzyme is an unspecific beta-glucanase able to break down both (1 leads to 3)-eta- and (1 leads to 6)-beta-linkages by an exo-splitting mechanism. Glucono-delta-lactone, Zn2+ and Hg2+ inhibited the enzyme activity.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002175:Candida / Q000201:enzymology*; D002474:Cell-Free System; D002850:Chromatography, Gel; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008970:Molecular Weight
chemical_list: D000596:Amino Acids; D006026:Glycoside Hydrolases