[Antivitamin activity of thiaminase from freshwater bicuspid molluscs after parenteral administration of the enzyme to albino mice].
Thiaminase from bicuspid mollusc, after a single administration into mice (25 or 50 un, subcutaneously), caused a decrease in the activity of transketolase and alpha-ketoglutaric acid dehydrogenase in the animal tissues within 3 hrs. The maximal antivitaminous effect of thiaminase was manifested within 2-5 days and slightly decreased within 7 days (at a dose of 25 un). Amount of thiamin was decreased in mice liver tissue from 6.54+/-0.36 mcg/g to 4.84+/-0.23 mcg/g within the second day after the thiaminase administration. Transketolase and alpha-ketoglutarate dehydrogenase from liver and kidney were more sensitive to the antivitaminous effect of thiaminase. General condition of the animals was not altered by parenteral administration of thiaminase during a week. Thiaminase may be recommended for modelling of thiamin deficiency in animals maintained at conventional diets.
D000818:Animals; D006867:Hydrolases / Q000008:administration & dosage / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D007655:Ketoglutarate Dehydrogenase Complex / Q000037:antagonists & inhibitors / Q000378:metabolism; D007656:Ketoglutaric Acids; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D051379:Mice; D008974:Mollusca / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D013154:Spleen / Q000201:enzymology; D013831:Thiamine / Q000037:antagonists & inhibitors*; D013997:Time Factors; D014174:Transketolase / Q000037:antagonists & inhibitors / Q000378:metabolism
D007656:Ketoglutaric Acids; D007655:Ketoglutarate Dehydrogenase Complex; D014174:Transketolase; D006867:Hydrolases; D013831:Thiamine
Antivitaminnaia aktivnost' tiaminazy iz dvustvorchatykh molliuskov pri parenteral'nom vvedenii fermenta belym mysham