[Characteristics of RNA isolated from the nuclear RNA-synthesizing complex of influenza virus].
When Ehrlich ascitic carcinoma cells infected with classical fowl plague virus and treated with actinomycin D were pulse labeled for 10 min with 3H-uridine, it was mainly incorporated into nucleoplasm structures sedimenting in sucrose gradients at 120S. At 2-hr exposure of the infected cells to 3H-uridine radioactivity was found in nucleoplasm in the area of 65S and in the cytoplasm in 30-40S zone. The analysis of RNA isolated from these structures gave the following results. The RNA isolated from 120S structures sedimented in two zones of sucrose gradient: 11S and 16-23S. The 11S RNA was resistant to RNA-ase, while 16-23S RNA was sensitive to RNA-ase. A similar (16-23S) RNA was isolated from virus-specific structures 65S and 30-40S.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis / Q000382:microbiology; D002384:Catalysis; D002467:Cell Nucleus / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D009980:Influenza A virus; D012367:RNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification; D012260:Ribonucleases; D014158:Transcription, Genetic; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication
D012367:RNA, Viral; D012260:Ribonucleases; D014529:Uridine
Kharakteristika RNK, vydelennoĭ iz iadernogo RNK-sinteziruiushchego kompleksa virusa grippa