[Interaction of electrophilic alkylating agents with nucleophilic sites of mitochondria].
The electrophilic alkylating agents having different numbers of electrophilic groups with nucleophilic sites of mitochondria were studied. Bifunctional compounds were found to modify the structure of mitochondria so that some of the sulfhydryl groups become inaccessible for titrating ions of Ag+. Monofunctional agents caused no changes in the number of determinable sulfhydryl groups and prevented the effect of bifunctional compounds. The linkage formed between the electrophilic residue and the nucleophilic site is stable in the absence of electron transport. During the transport of electrons in the respiratory chain the linkage becomes labile and the electrophilic residue chips off. A scheme of interaction of electrophilic alkylating agents with nucleophilic sites of mitochondria is proposed.
D000477:Alkylating Agents / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D004579:Electron Transport; D008930:Mitochondria, Liver / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000378:metabolism*; D008956:Models, Chemical; D011817:Rabbits; D051381:Rats; D012834:Silver; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000477:Alkylating Agents; D013438:Sulfhydryl Compounds; D012834:Silver
Vzaimodeĭstvie elektrofil'nykh alkiliruiushchikh agentov s nukleofil'nymi tsentrami mitokhondriĭ