The effect of withaferin A on human peripheral blood lymphocytes. An electron-microscope study.
Cultures of normal human peripheral blood lymphocytes stimulated by phytohaemagglutinin (PHA) were treated with withaferin A and examined by an electron microscope. The compound was found to affect the spindle microtubules of cells in metaphase. There were no intact pole-to-pole microtubules or pole-to-kinetochore microtubules found in the treated cells; the centrioles were located in the center. Furthermore, cells at interphase were also affected, the membrane system of the cells being damaged.
D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D002782:Cholestenes / Q000494:pharmacology*; D002783:Cholestenones / Q000494:pharmacology*; D007783:Lactones / Q000494:pharmacology; D037102:Lectins; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D008870:Microtubules / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D054358:Withanolides
D002782:Cholestenes; D002783:Cholestenones; D007783:Lactones; D037102:Lectins; D054358:Withanolides; C009684:withaferin A