title: The relationship between the uptake of glucose and 3-O-methylglucose and soluble carbohydrate and polysaccharide in the fungus Dendryphiella salina.
abstract: When mycelium of Dendryphiella salina, pre-incubated in D-[I-14C]mannitol such that this is the only major labelled soluble carbohydrate present, absorbs glucose or the non-metabolized sugar 3-O-methylglucose, there is a specific stimulation of incorporation of 14C into alkali-insoluble, trichloroacetic-acid-soluble (1 leads to 4)-alpha-glucan, probably glycogen. There is also a net increase in the amount of glucan caused by stimulation of synthesis and inhibition of breakdown. Addition of 3-O-methylglucose results in the loss of mannitol into the medium. This loss and the reduced rate of replenishment from the glucan is important in the osmotic regulation of the hyphae. If osmotic adjustment does not occur, the hyphae do not show the specific incorporation of 14C into the glucan.
mesh_terms: D050260:Carbohydrate Metabolism; D005947:Glucose / Q000378:metabolism*; D008353:Mannitol / Q000378:metabolism; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D003904:Mitosporic Fungi / Q000378:metabolism*; D009995:Osmosis; D011134:Polysaccharides / Q000378:metabolism*; D012623:Seawater; D012995:Solubility; D014871:Water Microbiology
chemical_list: D008757:Methylglucosides; D008759:Methylglycosides; D011134:Polysaccharides; D008353:Mannitol; D005947:Glucose