[Acceptor activity of 4-N-acetylcytidine in the synthesis of (3'-5')-internucleotide bond catalyzed by pancreatic nuclease].
Cytidine and 4-N-acetylcytidine were compared as phosphate acceptors in dinucleoside monophosphate synthesis catalyzed by pancreatic ribonuclease with uridine-2',3'-cyclophosphate and cytidine-2',3'-cyclo phosphate as phosphate donors. Because of low solubility of 4-N-acetylcytidine in water, the synthesis was carried out in aqueus-organic media. The results obtained indicate that acetylation of the exoaminogroup of cytidine decreases its acceptor activity. For the first time uridilyl-(3'-5')-4-N-acetylcytidine and cytidilyl-(3'-5')-4-N-acetylcytidine are prepared enzymatically by pancreatic ribonuclease.
D000107:Acetylation; D003562:Cytidine / Q000031:analogs & derivatives* / Q000378:metabolism*; D003597:Cytosine Nucleotides / Q000096:biosynthesis; D010179:Pancreas / Q000201:enzymology; D012260:Ribonucleases / Q000378:metabolism*; D014500:Uracil Nucleotides / Q000096:biosynthesis
D003597:Cytosine Nucleotides; D014500:Uracil Nucleotides; D003562:Cytidine; D012260:Ribonucleases
Aktseptornaia aktivnost' 4-N-atsetiltsitidina v sinteze (3'-5')-mezhnykleotidnoĭ sviazi, kataliziruemom pankreaticheskoĭ ribonukleazoĭ