Electron microscope assessment of fertilization of rabbit ova treated with concanavalin A and wheat germ agglutinin.
Zonaless rabbit ova, exposed to Concanavalin A or Wheat Germ Agglutinin, then to uterine capacitated sperm produce pronuclear, 2 and 4 stage embryos that are indistinguishable from controls. Absence of cortical granules indicates that the ova were fertilized and not merely activated. Survival of lectin-bearing receptors during the period necessary for fertilization was evaluated in ova marked with ferritin-conjugated lectin.
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D005260:Female; D005306:Fertilization; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D010063:Ovum / Q000187:drug effects* / Q000648:ultrastructure; D037121:Plant Lectins; D011817:Rabbits; D013075:Sperm Capacitation; D014908:Triticum
D037102:Lectins; D037121:Plant Lectins; D003208:Concanavalin A