title: [Mechanisms of spontaneous relaxation of smooth muscle (guinea pig taenia coli) depolarized in a hyperkalemic medium].
abstract: Experiments were performed on isolated strips of guinea pig taenia coli by the double sucrose-gap method. The artificial node was depolarized with potassium solution (from 120 to 167.7 mM KCl). When the bathing solution contained 0.4 mM Ca and the temperature was equal to 25 degrees C then potassium contracture was followed by fast relaxation. The muscular tone changed slightly during rectangular pulse of hyperpolarizing current, after switching off the current muscle generated a transient contractile response. The amplitude of such off-responses increased in some range with increasing in strength and duration of conditioning current. Treatment of muscle with compound D-600 resulted in a reduction of muscular tone and elimination of off-responses. The addition of Na ions to potassium solution (substitution of 47.7 mM KCl with the same quantity of NaCl) reduced muscular tone and enhanced the relaxation after off-responses. In sodium-free potassium solution each off-response was followed by increasing muscular tone but when the bathing solution contained Na ions this increase of the tone was not observed. The data obtained strongly suggest that the spontaneous relaxation of smooth muscle which was contracted in K-solution resulted from: 1) inactivation of calcium channels of surface membrane, 2) sequastration of Ca ions by intracellular storange sites, 3) extrusion of Ca in extracellular space (in part by means of Na-Ca exchange diffusion).
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D003106:Colon; D004058:Diffusion; D004558:Electric Stimulation; D006168:Guinea Pigs; D009126:Muscle Relaxation; D009130:Muscle, Smooth / Q000187:drug effects*; D011188:Potassium / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology
chemical_list: D012964:Sodium; D011188:Potassium; D002118:Calcium
vernacular_title: O mekhanizmakh samoproizvol'nogo rasslableniia depoliarizovannoi v giperkalievoĭ srede gladkoĭ myshtsy (taenia coli morskoĭ svinki)