Use of a tissue sectioner to expose internal structures of biological samples for scanning electron microscopy.
A procedure yielding sections of unembedded biological samples for observation by scanning electron microscopy is described. Sections of samples, fixed and hardened in OsO4, were obtained in quantity with a tissue sectioner. Subsequent treatments to osmium-coat cut surfaces were employed prior to critical point drying. The procedure yields cleanly cut surfaces through cells and cytoplasmic organelles which are retained in their normal position. Sections of apple leaf and mouse kidney are illustrated. Sections can be readily cut in a desired plane with less structural damage than is typically encountered by other sectioning or dissection techniques.
D000818:Animals; D007668:Kidney / Q000648:ultrastructure; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008867:Microtomy; D010944:Plants / Q000648:ultrastructure