[Cultivation and study of the properties of the Perego strain of the sheep poxvirus on heterologous tissue].
Strain Perego of the sheep pox virus, after 11 passages in lamb testis cultures, was successfully adapted and cultivated in 10 successive passages in a heterologous tissue--secondary cultures of newborn calf kidney. The infected cultures produced a cytopathic effect similar to that observed in a homologous culture, the difference being that part of the degenerated cells came off from the wall of the flask. At the level of its 10th passage the virus exhibited a titer of 10(3)CPE50. The virus was tested for harmlessness on sheep through a subcutaneous inoculation at a dose of 1 cm3 of a 1:10 dilution, and its testing for activity (1:100 dilution) caused an easily tolerated local reaction with a slight rise in the body temperature, the general status of the test animals remaining unaffected.
D000818:Animals; D003470:Culture Media; D011212:Poxviridae / Q000254:growth & development* / Q000648:ultrastructure; D012756:Sheep; D013997:Time Factors; D014611:Vaccination; D014776:Virus Cultivation / Q000379:methods
D003470:Culture Media
Kultivirane i prouchvane kachestvata na virusa na sharkata po ovtsete shtam Perego v kheterolozhna tukan