title: The presence of serine protease in pea embryo chromatin.
abstract: 1. It has been shown that chromatin from pea seedlings contains a proteolytic enzyme, similar to that of mammalian chromatin. 2. The protease was isolated from chromatin by acid extraction and partly characterized. It is a serine-type enzyme, sensitive to DFP, of low mol. wt. (about 18 000 - 20 000), with optimum pH at about 8 with [3H]N-acetylated histone as a substrate. 3. In chromatin complex, histones fl and f3 are preferentially degraded.
mesh_terms: D002843:Chromatin / Q000201:enzymology; D004586:Electrophoresis; D010450:Endopeptidases / Q000302:isolation & purification*; D006657:Histones / Q000378:metabolism; D008970:Molecular Weight; D012639:Seeds / Q000201:enzymology*; D012694:Serine
chemical_list: D002843:Chromatin; D006657:Histones; D012694:Serine; D010450:Endopeptidases