Concanavalin A induces microtubule assembly and specific granule discharge in human polymorphonuclear leukocytes.
Human neutrophils stimulated by concanavalin A (Con A, 100 microng/ml) contained markedly enhanced numbers of microtubules and discharged peroxidase-negative (specific) but not peroxidase-position (azurophile) granules. Release of lysozyme from specific granules was dose and time dependent, could be inhibitied by alpha-methyl-D-mannoside, and enhanced by cytochalasin B. Many microtubules were associated with internalized plasma membrane bearing Con A binding sites.
D002470:Cell Survival; D003208:Concanavalin A / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008766:Methylmannosides / Q000494:pharmacology; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D013696:Temperature
D008766:Methylmannosides; D003208:Concanavalin A; D003571:Cytochalasin B; D009113:Muramidase