[Incorporation of 32P-orthophosphate in aortic phospholipids in chronic lathyrims in the rat].
Study of 32P orthophosphate incorporation into rat aortic phospholipids after B.A.P.N. treatment, which was followed or not by a lipid-supplemented diet (4.5 p. 100 cholesterol and 37 p. 100 butter). The 32P orthophosphate turnover in the total phospholipid was twice as slow among B.A.P.N.-treated rat than among control-rat. Such a decrease of the 32P orthophosphate turnover could be found as well in phosphatidylethanolamines, phosphatidylcholines and lysophosphatidylcholines. Sphingomyelins showed a very weak metabolic activity among two groups of rats. 32P orthophosphate incorporation into inositolphosphatides was slowed down by B.A.P.N. treatment. Maximal specific activity of inositolphosphatides after injection of 32P orthophosphate was reached within 4 hours in control rats, and 8 hours in B.A.P.N.-treated rats. Three months after the end of B.A.P.N. treatment, no difference in specific activity could be found between control-rats and B.A.P.N.-treated rats. Lipid supplemented diet modified the metabolic activity of phospholipids equally in control rats and B.A.P.N.-treated rats. Specific activity decrease in total phospholipids, phosphatidylcholines, phosphatidylethanolamines, and increase in lysophosphatidylcholines were noted in both groups of animals under a high rat diet. These modifications of the metabolic activity of different phospholipids were most marked fourteen hours after 32P orthophosphate injection. B.A.P.N. treatment only slowed the incorporation of 32P phosphate into phospholipids. Administration of a single lipid supplemented diet or a diet associated with B.A.P.N. treatment induced a slower turnover of phosphatidylcholines and phosphatidylethanolamines, and a faster turnover of lysophosphatidylcholines.
D000629:Aminopropionitrile / Q000627:therapeutic use; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002908:Chronic Disease; D004041:Dietary Fats; D007842:Lathyrism / Q000188:drug therapy / Q000378:metabolism*; D008244:Lysophosphatidylcholines / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D051381:Rats; D013109:Sphingomyelins / Q000378:metabolism
D004041:Dietary Fats; D008244:Lysophosphatidylcholines; D010710:Phosphates; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D013109:Sphingomyelins; D000629:Aminopropionitrile
Incorporation  de 32P-orthophosphate dans les phospholipides aortiques au cours du lathyrisme chronique chez le rat