The nutrient requirements of MRC-5 human diploid cells.
Nutrients were assayed by their effect on maximum cell yield (maximum cell population less inoculum) of MRC-5 cells previously maintained in Eagle's basal medium with 10% (v/v) serum, trypsinised, centrifuged and washed. When nonlimiting amounts of iron, methylcellulose and a 68-component supplement were included in our defined medium, cell yields obtained were equivalent to those obtained with 2 to 3% whole serum. Growth then became limited by serum growth factors which, when serum was fractionated by the low temperature ethanol procedure, appeared to precipitate with the alpha-globulins, although the distribution of activity varied from batch to batch of serum. Column chromatography (DEAE and concanavalin A-Sepharose) of serum resulted in much (20 to 45%) loss of growth factor activity during exhaustive dialysis or Sephadex G-25 desalting suggesting that the growth factor may be a diffusible molecule bound in serum to a large protein carrier.
D002478:Cells, Cultured / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism*; D004171:Diploidy; D006801:Humans; D007501:Iron / Q000378:metabolism; D008747:Methylcellulose / Q000378:metabolism
D003470:Culture Media; D008747:Methylcellulose; D007501:Iron