Effects of monoolein on hydrolysis of triglyceride by lipoprotein lipase in the presence of an inhibitory apolipoprotein.
The previously demonstrated inhibition of cow's milk lipoprotein lipase by apoLp-Ala and the deinhibition by monoglyceride have been studied in more detail. The apoLp-Ala inhibition is reversible by the addition of monoglyceride before or after enzyme additions. Quantities of monoglyceride accumulate during hydrolysis of triglyceride which are adequate to prevent inhibition by added apoLp-Ala. Accelerating reaction rates observed when the substrate contains the apoprotein at levels producing partial inhibition are also explained by monoglyceride production. These effects were observed with both crude preparations of skim milk and highly pruified lipase. It is suggested that the generation of monoglyceride may be important in facilitating hydrolysis of triglyceride in lipoproteins containing this inhibitory apolipoprotein.
D000818:Animals; D001053:Apolipoproteins / Q000494:pharmacology*; D002417:Cattle; D002855:Chromatography, Thin Layer; D004347:Drug Interactions; D005989:Glycerides / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008892:Milk / Q000201:enzymology; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism*
D001053:Apolipoproteins; D005989:Glycerides; D014280:Triglycerides; C005953:monoolein; D008071:Lipoprotein Lipase