An anomaly in the resonance Raman spectra of cytochrome P-450cam in the ferrous high-spin state.
Resonance Raman spectra of cytochrome P-450cam (P-450cam) and its enzymatically inactive form (P-420) in various oxidation and spin states were measured for the first time. The Raman spectrum of reduced P-450cam was unusual in the sense that the "oxidation-state marker" appeared at an unexpectedly lower frequency (1346 cm-1) in comparison with those of other reduced hemoproteins (approximately 1355-approximately 1365 cm-1), whereas that of oxidized P-450cam was located at a normal frequency. This anomaly in the Raman spectrum of reduced P-450cam can be explained by assuming electron delocalization from the fifth ligand, presumably a thiolate anion, to the antibonding pi orbital of the porphyrin ring. The corresponding Raman line of reduced P-420 appeared at a normal frequency (1360 cm-1), suggesting a status change or replacement of the fifth ligand upon conversion from P-450cam to P-420. The Raman spectrum of reduced P-450cam-metyrapone complex was very similar to that of ferrous cytochrome b5.
D003577:Cytochrome P-450 Enzyme System; D005296:Ferrous Compounds; D006420:Hemeproteins; D010084:Oxidation-Reduction; D011549:Pseudomonas; D013059:Spectrum Analysis, Raman
D005296:Ferrous Compounds; D006420:Hemeproteins; D003577:Cytochrome P-450 Enzyme System