title: Total plasmalogens and O-(acylalkylglycerophosphoryl) ethanolamine from labelled hexadecanol and palmitate during hypoxia and anoxia in rat heart.
abstract: By the use of the Langendorff technique, surviving isolated rat hearts were perfused with [1-14 C] palmitate, [1-14C] hexadecanol or [1-14C,1-3H] hexadecanol under normal or anoxic conditions. After perfusion for 30min with either precursor, when oxygenated or in an hypoxic condition, or when 1mM-KCN was included in the system, the heart tissues showed no significant chemical changes in their content of total lipids, total phospholipids or total ethanolamine-containing phospholipids. Changes were observed in the ratio of alkyl-to alk-1-enyl-glycerophosphorylethanolamine in the tissue perfused with N2+CO1 plus CN-. A slight increase from 4.0+/-0.3 to 4.9+/-0.2% in alkyl derivatives and a decrease from 11.2+/-0.4 to 9.4+/-0.3% in alk-1-enyl derivatives was observed. The incorporation of the [14C] palmitate and the [14C] hexadecanol into the recovered phospholipids and plasmalogens was severely decreased in the tissues perfused with CN-: in the hypoxic state only a mild inhibition was observed compared with the oxygenated systems. Considerable 3H from [1-14C, 1-3H] hexadecanol was retained (25-35%) in the alk-1-enylether chains of plasmalogens under both the oxygenated conditions and with CN-, suggesting that the same mechanism of incorporation is operational at high or low O2 concentrations. The results are consistent with an O2-dependent, CN-sensitive step in the biosynthesis of plasmalogens in the rat heart.
mesh_terms: D000438:Alcohols / Q000378:metabolism; D000818:Animals; D000860:Hypoxia / Q000378:metabolism*; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010100:Oxygen; D010168:Palmitates / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010955:Plasmalogens / Q000096:biosynthesis*; D051381:Rats
chemical_list: D000438:Alcohols; D010168:Palmitates; D010714:Phosphatidylethanolamines; D010955:Plasmalogens; D010100:Oxygen