[Automatic ultramicrotechnic for the determination of plasma uric acid].
An automatic micromethod is described for the estimation of plasma uric acid. The hydrogen peroxide formed when uric acid is oxidised by uricase, condenses by oxidation two molecules of p-hydroxyphenil-acetic acid in the presence of peroxidase. The product formed (dicarboxymethyl-5,5' dihydroxy-2,2' biphenyl) is fluorescent. This method permits one to estimate quantitites of uric acid of the order of 1 microng and permits measurement of uric acid in 50 microll of plasma. The values obtained for human plasma are compared to those supplied by a reductrimetric technique (SMA 12-60).
D001322:Autoanalysis; D001713:Biphenyl Compounds; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D008832:Microchemistry / Q000379:methods*; D010648:Phenylacetates; D013050:Spectrometry, Fluorescence; D014503:Urate Oxidase / Q000378:metabolism; D014527:Uric Acid / Q000097:blood*
D001713:Biphenyl Compounds; D010648:Phenylacetates; D014527:Uric Acid; D006735:Horseradish Peroxidase; D014503:Urate Oxidase
Ultramicrométhode automatisée de dosage de l'acide urique plasmatique