The effect of specific antiserum on the metabolism of three membrane-associated antigens of ASL-1 x LM(TK)- hybrid cells.
Fusion of ASL-1 cells, a murine leukemia forming thymus leukemia (TL) antigens, with LM(TK)- cells, a TL(--) murine cell line, resulted in a stable hybrid forming TL antigens. The hybrids failed to undergo modulation, the reversible dissappearance of TL antigens from the surfaces of the cells, stimulated by TL antiserum. Unlike ASL-1 cells, the rate of disappearance of the antigens from modulation negative hydrid cells was unaffected by TL antiserum. The t 1/2 of TL antigens of the hybrid was approximately 30 h. The t 1/2 of TL antigens of ASL-1 cells was 10 h in the presence of TL antiserum, 18 h in the absence of TL antiserum. The rate of metabolism of a putative tumor-associated antigen of ASL-1 cells formed by the hybrid was unaffected by exposure to specific antiserum, as was the metabolism of H-2 antigens formed by the cell types.
D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D002462:Cell Membrane / Q000276:immunology*; D006822:Hybrid Cells / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology*; D066298:In Vitro Techniques; D007942:Leukemia, Experimental / Q000276:immunology / Q000473:pathology*; D051379:Mice
D000951:Antigens, Neoplasm; D007106:Immune Sera