Metabolites of estradiol-17beta in guinea pig uterus late in pregnancy.
The metabolism of estradiol-17beta by the guinea pig uterus late in pregnancy was studied in vivo and in vitro. Whole uteri were examined for estrogen metabolites one hour following an intravenous injection of [3H]-estradiol-17beta or uterine sections were examined after incubation for one hour at 37 degrees C in medium containing [3H]-estradiol-17beta. In both instances uterine tissue metabolized estradiol-17beta to five products; estrone, estrone-3-sulfate, 17beta-estradiol-3-sulfate, estrone-3-glucuronide and 17beta-estradiol-3-glucuronide. Of the total radioactive products 11-43% were glucuronides, 17-26% were sulfates and 4-17% was estrone. These results indicate that the guinea pig uterus actively transforms estradiol-17beta into glucuronides and sulfates late in pregnancy.
D000818:Animals; D004958:Estradiol / Q000378:metabolism*; D004970:Estrone / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D005965:Glucuronates / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D011247:Pregnancy; D013431:Sulfates / Q000378:metabolism; D014599:Uterus / Q000378:metabolism*
D005965:Glucuronates; D013431:Sulfates; D004970:Estrone; D004958:Estradiol