125I-Radioimmunoassay of amikacin and comparison with a microbioassay.
A radioimmunoassay (RIA) has been developed using 125I-amikacin. Amikacin was iodinated by a modified BOLTON and HUNTER method. Dextran-charcoal was used to separate bound from free drug. The standard curve was linear on a logit-log plot in the range of 0.5 ng to 4 ng amikacin per tube. There was no cross-reactivity of amikacin antisera to the amino-glycosides gentamicin, tobramycin, netilmicin, and sisomicin but a 70% cross-reaction was observed with kanamycin, the compound from which amikacin is synthetically derived. Correlation of the RIA with a microbioassay for the determination of serum amikacin levels in 18 patient samples was excellent (r = 0.94). This new RIA technique is more sensitive, rapid, versatile, and less costly than the RIA using 3H-amikacin, and is far more sensitive and faster than microbioassay.
D000583:Amikacin / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D001681:Biological Assay; D007114:Immunization; D007457:Iodine Radioisotopes; D007612:Kanamycin / Q000031:analogs & derivatives*; D008722:Methods; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay
D007457:Iodine Radioisotopes; D007612:Kanamycin; D000583:Amikacin