[Preparation and immunoephelometric quantitation of fibrinogen in rabbits].
Pure rabbit fibrinogen was prepared by a method involving two ammonium sulfate precipitations, one 2 M phosphate buffer precipitation, one DEAE cellulose chromatography and lastly one Sepharose 6 B chromatography. The aminoacid composition was determined and an immunonephelemetric assay was proposed. This assay followed an accurate determination of fibrinogen concentration in a rabbit with inflammatory reaction.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005340:Fibrinogen / Q000302:isolation & purification; D007122:Immunoelectrophoresis; D008297:Male; D011817:Rabbits
D000596:Amino Acids; D005340:Fibrinogen
Préparation et dosage immunonéphélémétrique du fibrinogène de lapin