[Incorporation of labeled precursors in glycosaminoglycans of the media-intima of the arterial wall].
The enzymes involved in glycosaminoglycans biosynthesis are characterized in homogenates of aortic media-intima. Incorporation of hexosamine from uridine nucleotide precursor was done into the saccharide chains of glycosaminoglycans. Labelling of aortic glycosaminoglycans by 35S-precursors is performed in our fractions; the results obtained demonstrate the uptake of 35S-sulfate from its corresponding active nucleotide sulfate ; highest sulfation is obtained for the post-microsomal supernatant. Following incorporation of 35S-sulfate or 14C-radio-activity into the different glycosaminoglycans, the largest percent uptake is obtained for heparan sulfate and the lowest for chondroitin sulfate.
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002250:Carbon Radioisotopes / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006497:Heparan Sulfate / Q000378:metabolism; D006595:Hexosamines / Q000378:metabolism; D007553:Isotope Labeling; D013462:Sulfur Radioisotopes / Q000378:metabolism; D013552:Swine
D002250:Carbon Radioisotopes; D002809:Chondroitin Sulfates; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006595:Hexosamines; D013462:Sulfur Radioisotopes
Incorporation de précurseurs marqués dans les glycosaminoglycanes de média-intima de paroi artérielle