[Physicochemical properties of the human lymphocyte mitogenic factor induced by phytohemagglutinin].
Some physical and chemical properties of the mitogenic factor (MF) produced in vitro by PHA-stimulated human lymphocytes were investigated. Treatment of the culture medium with proteases proved to decrease the MF activity sharply. The MF was found to have an elution pattern in Sephadex and Bio-gel gels similar to proteins with the molecular weight of 20 000--30 000 daltons (peak--25 000). In experiments with the MF fractionation by disc-electrophoresis on 5% polyacylamide gel the MF formed a few discrete peaks in the fractions corresponding to the mobility of alpha1- and alpha2-globulins and transferrin. In isoelectric focusing the MF formed 3 fractions in the pH range of 4.5--8.3. Functional heterogeneity of the MF is suggested.
D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D037102:Lectins / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects*; D008934:Mitogens / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000494:pharmacology
D037102:Lectins; D008934:Mitogens; D010447:Peptide Hydrolases
Fiziko-khimicheskie svoĭstva mitogennogo faktora limfotsitov cheloveka, indutsirovannogo fitogemaggliutininom