Estimation of the Ca2+ binding by microsomal membranes from pig coronary artery using a calcium ion selective electrode.
To estimate the Ca2+ binding of microsomal membranes from pig coronary artery a modified potentiometric method was used. With this method it was possible to follow directly changes in the Ca2+ ion activity of the incubation medium down to 10(-8) moles. Compared with the Ca45-filter technique the electrometrically measuring device built up was beside the obvious advantages of a directly indicating method more fast, simple and hightly sensitive.
D000818:Animals; D001158:Arteries / Q000378:metabolism* / Q000648:ultrastructure; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003331:Coronary Vessels / Q000378:metabolism* / Q000648:ultrastructure; D008861:Microsomes / Q000378:metabolism*; D011199:Potentiometry; D013552:Swine / Q000378:metabolism*
D002118:Calcium