[Detection of components of type D oncornavirus in the mitochondria of HEp-2 cells].
The HEp-2 cell mitochondria were found to harbor electronoptically dense subvirus particles with a byouant density of 1.27-1.28 g/cm3 which formed in the mitochondria de novo (experiments on incubation of isolated mitochondria with 32P). The intramitochondrial localization of proteins of oncornavirus type D was proven by different methods of the antigenic analysis (CFT, immune precipitation, autoradiography). The additional evidence of the participation of mitochondria in biosynthesis of this virus was obtained by the use of inhibitory tests (a different effect of chloramphenicol and cycloheximide). The possible role of mitochondria in biosynthesis of oncornaviruses is discussed.
D000956:Antigens, Viral / Q000032:analysis*; D000998:Antiviral Agents / Q000494:pharmacology; D002478:Cells, Cultured; D008854:Microscopy, Electron; D008928:Mitochondria / Q000276:immunology / Q000382:microbiology*; D009858:Oncogenic Viruses / Q000302:isolation & purification*; D014779:Virus Replication / Q000187:drug effects
D000956:Antigens, Viral; D000998:Antiviral Agents
Obnaruzhenie komponentov onkornavirusa tipa D v mitokhondriiakh kletok HEp-2