[Nature of the effect on RNA and substrate specificity of RNAase of Bacillus amylozyma 9a].
Extracellular RNAase from Bac. amylozyma 9a has endonucleolytic character of the action on RNA, it splits in RNA 5'-phosphodiester bonds of GpXp type (where X is any nucleoside). The hydrolysis proceeds in two steps by the intramolecular transphosphorylation type of reaction to form guanosine-2',3'-phosphates and with the subsequent hydrolysis of cyclic bonds. The enzyme shows a preferable specificity to the single-stranded structure of the polyribonucleotides.
D001407:Bacillus / Q000201:enzymology*; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D006868:Hydrolysis; D009711:Nucleotides / Q000302:isolation & purification; D012313:RNA / Q000032:analysis*; D012260:Ribonucleases / Q000302:isolation & purification / Q000494:pharmacology*
D009711:Nucleotides; D012313:RNA; D012260:Ribonucleases
Kharakter deĭstviia na RNK i substratnaia spetsifichnost' RNKazy Bacillus amylozyma 9a