DNA damage and repair in intact animals. Single-stranded regions in rat liver DNA following administration of dimethylnitrosamine.
Rat liver DNA was radioactively labelled by administration of [3H]thymidine following partial hepatectomy. Two weeks later, the rats were treated with the carcinogen, dimethylnitrosamine. DNA was isolated and fractionated by elution from benzoylated DEAE-cellulose with NaCl and caffeine solution. The caffeine-eluted fraction was increased by administration of dimethylnitrosamine. This increase was proportional to the dose of carcinogen injected and persisted for at least 24 h after administration of the carcinogen. These data, together with the results of hydroxyapatite chromatography, suggest that the DNA contains short single-stranded sections associated with much longer regions of native DNA.
D000818:Animals; D004260:DNA Repair / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis*; D004128:Dimethylnitrosamine / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000032:analysis / Q000187:drug effects*; D009602:Nitrosamines / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D009602:Nitrosamines; D004128:Dimethylnitrosamine