title: An isolated perfused rat lung preparation.
abstract: An isolated perfused rat lung preparation (IPL) is described and its physiologic status is evaluated. The evaluation includes light and electron microscopy after perfusion and estimations of substrate utilization. ATP content, lactate production, and incorporation of glucose carbons into lipids and CO2. It is concluded that the IPL is useful for short-term metabolic and physiologic experiments and offers some unique advantages in evaluating effects of reactive gases upon lung function.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000758:Anesthesia; D000818:Animals; D001808:Blood Vessels / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism / Q000502:physiology* / Q000601:surgery; D008196:Lymph / Q000502:physiology; D008264:Macrophages / Q000378:metabolism; D008722:Methods; D010477:Perfusion; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D012119:Respiration; D012121:Respiration, Artificial
chemical_list: D011773:Pyruvates; D000255:Adenosine Triphosphate; D005947:Glucose