title: Freeze-drying of a purified human diploid cell rabies vaccine.
abstract: A rabies vaccine prepared in human diploid cell strains was purified by continuous-flow sucrose density gradient centrifugation. The peak fractions containing 35-40% sucrose were diluted with a stabilizer consisting of degraded gelatine in a Tris-EDTA-buffer, and then inactivated with beta-propiolactone and freeze-dried to a residual moisture of approximately 2.5%. This vaccine was stable as proved by accelerated stability tests.
mesh_terms: D002460:Cell Line; D004171:Diploidy; D004355:Drug Stability; D005612:Freeze Drying; D006801:Humans; D011819:Rabies Vaccines / Q000592:standards; D011820:Rabies virus / Q000276:immunology / Q000302:isolation & purification; D013696:Temperature; D014776:Virus Cultivation
chemical_list: D011819:Rabies Vaccines