[Method of determining the intregral activity of catechol-O-methyltransferase in the human body in vivo].
A method is developed for estimation of the catecholamine-O-methyl transferase activity in human organism by excretion with urine of unmetabolized isadrine (N-isopropyl noreadrenaline) after loading. Excretion of isadrine was increased both after administration of apomorphine, which is one of the inhibitors of the enzyme catecholamine-O-methyl transferase, and in cases of liver tissue pathology. It is known that the maximal activity of catecholamine-O-methyl transferase is the highest in liver tissue. Imipramine (an inhibitor of uptake of catecholamine) did not inhibit the activity of cafecholamine-O-methyl transferase as estimated by the present method.
D001058:Apomorphine / Q000494:pharmacology; D002394:Catechol O-Methyltransferase / Q000032:analysis* / Q000378:metabolism; D065098:Catechol O-Methyltransferase Inhibitors; D006801:Humans; D007099:Imipramine / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008722:Methods
D065098:Catechol O-Methyltransferase Inhibitors; D002394:Catechol O-Methyltransferase; D007545:Isoproterenol; D001058:Apomorphine; D007099:Imipramine
Metod opredeleniia integral'noĭ aktivnosti katekhol-O-metiltransferazy v organizme cheloveka in vivo