[Isolation of the most active DNA synthesizing cells of hematopoietic organs by fractionation on a density gradient of albumin].
After separation of the bone marrow or the spleen suspension on the discontinuous albumin gradient cell fractions were obtained in which the activity of the DNA-dependent DNA polymerase, aspartate carbamoyl transferase, as well as the rate of the 14C-thymidine incorporation in the DNA was 2 to 3 times higher than in the original suspension. The most actively DNA-synthesizing cells were concentrated in the 5th-6th fractions when the osmolarity of 35% BSA was 370 mOsm, or in the 2nd-3rd fractions when the osmolarity of 35% BSA was 380 mOsm.
D000818:Animals; D001221:Aspartate Carbamoyltransferase / Q000378:metabolism; D001853:Bone Marrow / Q000201:enzymology / Q000378:metabolism; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods; D004247:DNA / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D051381:Rats; D012710:Serum Albumin, Bovine; D013154:Spleen / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism
D012710:Serum Albumin, Bovine; D004247:DNA; D001221:Aspartate Carbamoyltransferase; D004259:DNA-Directed DNA Polymerase
Vydelenie naibolee aktivno sinteziruiushchikh DNK kletok krovetvornykh organov putem fraktsionirovaniia v gradiente plotnosti al'bumina