Chemical and biochemical studies for the differentiation of coagulase-positive staphylococci.
The cell wall composition, the configuration of lactic acid produced from glucose under anaerobic conditions, the occurrence of fructose-1,6-diphosphate (FDP) activated L-lactate dehydrogenase (L-LDH), and the esterase pattern were determined from more than 80 strains of coagulase-positive staphylococci isolated from man and animal. Strains isolated from man, swine, bovines and hares form a rather homogeneous group. They exhibit a similar cell wall composition, produce predominantly D,L-lactate and have a characteristic and simple esterase pattern. Coagulase-positive staphylococci isolated from dogs, horses, minks and pigeons are quite distinct from typical Staphylococcus aureus strains. They exhibit a different cell wall composition, produce only L-lactate, possess an L-LDH which is specifically activated by FDP, and have a quite complex esterase pattern.
D000117:Acetylglucosamine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002473:Cell Wall / Q000032:analysis; D003030:Coagulase; D005947:Glucose / Q000032:analysis; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D007773:Lactates / Q000032:analysis; D009112:Muramic Acids / Q000032:analysis; D009283:Naphthol AS D Esterase / Q000032:analysis; D013211:Staphylococcus aureus / Q000032:analysis / Q000145:classification* / Q000201:enzymology; D013237:Stereoisomerism
D000596:Amino Acids; D003030:Coagulase; D007527:Isoenzymes; D007773:Lactates; D009112:Muramic Acids; D007770:L-Lactate Dehydrogenase; D009283:Naphthol AS D Esterase; D005947:Glucose; D000117:Acetylglucosamine