[Enzymatic splitting of antibodies bound to immobilized antigen as a means of Fab fragments preparation].
A method of Fab fragments preparation by enzymatic splitting of antibodies bound to specific antigen immobilized on an insoluble support is described. The complex of rat muscle glyceraldehyde-3-phosphate dehydrogenase (GAPD), immobilized on Sepharose 4B, with anti-rat GAPD rabbit antibodies was digested with papain. The antigen was inaccessible to proteolysis under conditions employed. After 4 hrs of incubation with papain the antibody was completely split into non-precipitating fragments. The products of proteolysis not bound to Sepharose, were eluted with 0.1 M givcine buffer pH 2.5, and shown to correspond to Fab fragments.
D000818:Animals; D000906:Antibodies; D055598:Chemical Phenomena; D002621:Chemistry; D004800:Enzymes, Immobilized; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments; D009132:Muscles / Q000201:enzymology; D010206:Papain; D051381:Rats
D000906:Antibodies; D004800:Enzymes, Immobilized; D007140:Immunoglobulin Fab Fragments; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010206:Papain
Fermentativnoe rasshcheplenie antitel, sviazannykh s immobilizovannym antigenom, kak sposob polucheniia Fab-fragmentov