Effect of L-azetidine-2-carboxylic acid on glycosylations of collagen in chick-embryo tendon cells.
The glycosylations of hydroxylysine during collagen biosynthesis in isolated chick-embryo tendon cells were studied by using pulse-chase labelling experiments with [14C]-lysine. The hydroxylation of lysine and the glycosylations of hydroxylysine continued after a 5 min pulse label for up to about 10 min during the chase period. These data differ from those obtained previously in isolated chick-embryo cartilage cells, in which, after a similar 5 min pulse label, these reactions continued during the chase period for up to about 20 min. The collagen synthesized by the isolated chick-embryo tendon cells differed markedly from the type I collagen of adult tissues in its degree of hydroxylation of lysine residues and glycosylations of hydroxylysine residues. When the isolated tendon cells were incubated in the presence of L-azetidine-2-carboxylic acid, the degree of glycosylations of hydroxylysine during the first 10 min of the chase period was identical with that in cells incubated without thcarboxylic acid for at least 60 min, whereas no additional glycosylations took place in the control cells after the 10 min time-point. As a consequence, the collagen synthesized in the presence of this compound contained more carbohydrate than did the collagen synthesized by the control cells. Additional experiments indicated that azetidine-2-carboxylic acid did not increase the collagen glycosyltransferase activities in the tendon cells or the rate of glycosylation reactions when added directly to the enzyme incubation mixture. Control experiments with colchicine indicated that the delay in the rate of collagen secretion, which was observed in the presence of azetidine-2-carboxylic acid, did not in itself affect the degree of glycosylations of collagen. The results thus suggest that the increased glycosylations were due to inhibition of the collagen triple-helix formation, which is known to occur in the presence of azetidine-2-carboxylic acid.
D000818:Animals; D001383:Azetidinecarboxylic Acid / Q000378:metabolism*; D001385:Azetines / Q000378:metabolism*; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology; D003094:Collagen / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000378:metabolism; D006900:Hydroxylation; D006901:Hydroxylysine / Q000031:analogs & derivatives / Q000096:biosynthesis; D066298:In Vitro Techniques; D013710:Tendons / Q000166:cytology* / Q000378:metabolism
D001385:Azetines; D006901:Hydroxylysine; D001383:Azetidinecarboxylic Acid; D003094:Collagen; D005964:Glucosyltransferases; D003078:Colchicine