Polyamine-dependent deoxyribonuclease activity from rat-liver nuclei.
When nuclei isolated from rat liver in a low salt buffer were washed with 0.1 M NaCl solution, the supernatant showed a deoxyribonuclease (DNase) activity. The activity required Mg2+ and in addition spermine or spermidine, and its optimal pH was 7.2-7.4. The activity was higher on denatured (single stranded) DNA than on double-helical DNA. With both substrates the activity was highest at a polyamine concentration at which the DNA-polyamine complex began to precipitate. No Mg2++Ca2+ dependent DNase activity was detected in the preparation.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002467:Cell Nucleus / Q000201:enzymology*; D004247:DNA / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D003956:Dialysis; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D011073:Polyamines / Q000494:pharmacology*; D051381:Rats; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D011073:Polyamines; D013096:Spermine; D004247:DNA; D003851:Deoxyribonucleases; D008274:Magnesium; D002118:Calcium; D013095:Spermidine