[Binding and distribution of chlordiazepoxide (Radepur) and its metabolites in the erythrocyte-plasma and erythrocyte-buffer systems].
The distribution and the free concentration of chlorodiazepoxide, desmethyl-chlorodiazeoxide and demoxepam in the systems erythrocyte-plasma and erythrocyte-buffer is determined. In both systems the substances are bound by erythrocytes. A constant ratio of distribution between erythyrocyte-plasma and erythrocyte-buffer, respectively, is established beyond therapeutic concentrations. The association constants (dual reciprocal plot) of these substances are of the order of 10(4)-1-M-1. They are the same for erythrocytes and plasma. The specific binding of erythrocytes is 20-35 fold less than with plasma proteins. It is concluded that the erythrocytes are a binding intravascular subcompartment for chlorodiazepoxide and its metabolites.
D001711:Biotransformation; D001798:Blood Proteins / Q000378:metabolism*; D002021:Buffers; D002707:Chlordiazepoxide / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding
D001798:Blood Proteins; D002021:Buffers; D002707:Chlordiazepoxide
Uber Bindung und Verteilung von Chlordiazepoxid(Radepur) und seiner Metabolite im Erythrozyten-Plasma- und Erythrozyten-Puffer-System