[Certain aspects of transformation of 21-acetate of the Reichstein substance "S" by the culture of Tieghemella orchidis].
The transformation of acetate from the Reichstein substance "S" by the culture of Tieghemella orchidis was studied upon a single and fractional addition of the substrate. The effect of streptomycin sulphate, Tween-80, ethanol (steroid solvent) and steroid solution on the respiratory activity of the culture was investigated upon a single and fractional addition of the steroid. During transformation the respiratory activity of the culture decreased--by the end of the process (18-20 hours) it fell 2-2.5-fold upon a single addition of the steroid (1 g/l) and by 20% upon a fractional addition of the steroid (2 g/l). In the latter case the respiratory activity dropped step-by-step if the concentration of the steroid was 1.4-1.6 g/l; simultaneously the transformation activity declined.
D000085:Acetates / Q000378:metabolism*; D046508:Culture Techniques; D000431:Ethanol / Q000494:pharmacology; D005658:Fungi / Q000378:metabolism*; D006854:Hydrocortisone / Q000494:pharmacology; D009090:Mucorales / Q000378:metabolism*; D010101:Oxygen Consumption; D011136:Polysorbates / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology
D000085:Acetates; D011136:Polysorbates; D000431:Ethanol; D006854:Hydrocortisone; D013307:Streptomycin
Nekotorye aspekty transformatsii 21-atsetata veshchestva "S" Reĭkhshteina kul'turoĭ Tieghemella orchidis