[Purification and enzyme study of kininogens I and II from human plasma].
Kininogens I (HMW) and II (LMW) were isolated and partially purified from human plasma. The disappearance of kininogen I was prevented by the use of hexadimethrine bromide, which inhibits the activation of plasma prekallikrein. The two kininogens were separated by chromatography on DEAE-cellulose. Further purification of kininogen I and II was achieved by separate chromatographic steps of the partially purified kininogens on SP-Sephadex. The purification of the kininogens was controlled by incubation of the respective samples with different kininogenases: human plasma kallikrein, human salivary kallikrein and trypsin. As determined by gel filtration, a molecular weight of 250 000 daltons was found for kininogen I (HMW) and 48 000 daltons for kininogen II (LMW).
D004796:Clinical Enzyme Tests; D006801:Humans; D007610:Kallikreins / Q000097:blood / Q000378:metabolism; D007704:Kininogens / Q000097:blood / Q000302:isolation & purification*; D008722:Methods; D008970:Molecular Weight; D012469:Salivary Glands / Q000201:enzymology
D007704:Kininogens; D007610:Kallikreins
Purificacion y estudio enzimatico de los quininogenos I y II del plasma humano.