Studies on the bacteriophage PS8 of Agrobacterium tumefaciens (Smith and Townsend) Conn: physico-chemical properties of its DNA.
DNA from the bacteriophage PS8 was extracted and purified. The buoyant density was determined was 1.716 cm3/g. The guanine-cytosine content was calculated to be 57%. DNA molecules which looked like circles were found among linear strands in an electron-microscopic study. With an endonuclease from Streptomyces albus G the DNA was digested to 19 fragments, with molecular weights ranging from 600 to 7,400 daltons. The molecular weight of the DNA was determined to be 38.8 X 10(6) daltons +/- 8.7%.
D001435:Bacteriophages / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D004270:DNA, Circular / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D008970:Molecular Weight; D012231:Rhizobium
D004270:DNA, Circular; D004279:DNA, Viral