Cooperative binding of concanavalin A to thymocyte membrane vesicles augments uptake of alpha aminoisobutyrate.
(1) Membrane vesicles from rabbit thymocytes accumulate alpha-aminoisobutyrate in the presence of 0.1 M NaCl. Uptake is 1/2 maximal after about 2 min and reaches a plateau value (61 pmoles/mg protein) after 30 min. (2) Up to 25 mug concanavalin A/ml, binding of the lectin describes a sigmoid curve indicative of a cooperative process. (3) At lectin concentrations up to 8 mug/ml, lectin binding enhances the uptake of alpha-aminoisobutyrate (maximally 30%).
D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D003208:Concanavalin A / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits; D012965:Sodium Chloride / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology*
D000621:Aminoisobutyric Acids; D003208:Concanavalin A; D012965:Sodium Chloride