[Study of amino acid composition and biosynthesis of protein components of the membrane system of corn plastids during biogenesis of chloroplasts].
The biosynthesis of membrane proteins in maize plastids at different stages of differentiation of the chloroplast lamellar system was studied. Prolamellar and lamellar system preparations were isolated from maize plastids, disintegrated by osmotic shock under hypotonic conditions. Changes in the amino acid composition of 14C membrane proteins were observed at all stages of chloroplast ultrastructure formation. The maximal level of the apolar amino acids was observed in the membrane fraction of chloroplasts. Washed membranes from maize proplastids and chloroplasts can be resolved into at least 14 protein bands on formic acid--urea polyacrylamide gel. It is pointed out that biogenesis process leads to the increase of lipophylic protein content in the chloroplast lamellae fraction.
D000596:Amino Acids / Q000032:analysis*; D002250:Carbon Radioisotopes; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D001678:Organelle Biogenesis; D010940:Plant Proteins / Q000032:analysis / Q000096:biosynthesis*; D003313:Zea mays
D000596:Amino Acids; D002250:Carbon Radioisotopes; D008565:Membrane Proteins; D010940:Plant Proteins
Izuchenie aminokislotnogo sostava i biosinteza belkovykh komponentov membrannoĭ sistemy plastid pri biogeneze khloroplastov