[Biosynthesis of 14C- and 35S-lincomycin using different sources for the label].
The sources of radioactive labels were chosen for biosynthesis of labeled lincomycin. The levels of the label incorporation into lincomycin were high with all the sources used when the lincomycin-producing organism was cultivated on the synthetic medium as compared to the complex medium. Incorporation of the label into lincomycin was most effective when I14C- or 214C-thyrosine was used as the precursors. These precursors were effective in both the complex and the synthetic media. In production of significant amounts of the labeled lincomycin I14C- or 214C-sodium propionate, I14C- or 142C-sodium acetate and 14C-protein hydrolysate may be used as the sources of the radioactive carbon. In production of 35S-lincomycin K235SO4 may be used as the source of the label. The optimal conditions for biosynthesis of 14C- and 35S-lincomycin were developed (concentration of some components of the medium, time of the label addition and others).
D000085:Acetates / Q000378:metabolism; D002250:Carbon Radioisotopes; D002708:Chlorella; D003470:Culture Media / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism; D008034:Lincomycin / Q000096:biosynthesis*; D008715:Methionine / Q000378:metabolism; D011422:Propionates / Q000378:metabolism; D011492:Protein Hydrolysates / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D013997:Time Factors; D014443:Tyrosine / Q000378:metabolism
D000085:Acetates; D002250:Carbon Radioisotopes; D003470:Culture Media; D011422:Propionates; D011492:Protein Hydrolysates; D013431:Sulfates; D013462:Sulfur Radioisotopes; D014443:Tyrosine; D008715:Methionine; D008034:Lincomycin; D005947:Glucose
Biosintez 14C- i 35S-linkomitsina pri ispol'zovanii razlichnykh istochnikov metki