Cell surface properties of L1210 leukaemia cells.
The surface properties of vincristine-colchicine sensitive and resistant L1210 leukaemic cells have been studied using concanavalin A mediated agglutination assay as well as electron microscopic visualization of concanavalin A receptors. 3H-colchicine uptake by the sensitive and resistant lines has also been compared. The resistant L1210 leukaemic cells proved less agglutinable than the sensitive ones at the same concanavalin A concentration. Previous treatments with either colchicine, vincristine or chlorpromazine caused a marked decrease in the agglutinability of the sensitive L1210 leukaemic cells, while agglutination of the resistant ones was lowered slightly by the same treatments. The 3H-colchicine uptake of the sensitive cells was three times higher than that of the resistant ones.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003078:Colchicine / Q000378:metabolism; D004351:Drug Resistance; D066298:In Vitro Techniques; D007939:Leukemia L1210 / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D051379:Mice; D011952:Receptors, Concanavalin A; D013499:Surface Properties
D011952:Receptors, Concanavalin A; D003078:Colchicine