Biosynthesis of DNA, RNA and proteins by mouse embryos cultured in the presence of a teratogenic dose of chlorambucil.
The effect of chlorambucil on the rates of DNA, RNA, and protein synthesis in mouse embryos was investigated using a system of whole embryo culture. Embryos were isolated on the 11th day of gestation (33 +/- 3 somites) and grown in culture media for periods of 4-8 h. Reichert's membrane and most of the placental tissue was removed leaving only the amnion and visceral yolk-sac surrounding the embryo. In the presence of teratogenic doses of chlorabucil (15 mug/ml) the rate of DNA synthesis was significantly decreased at 4 and 8 h. RNA and protein synthesis were not inhibited at either of these times. A trend toward decreasing rates of protein synthesis at some time beyond 8 h was noted, but not tested.
D000818:Animals; D002699:Chlorambucil / Q000633:toxicity*; D046508:Culture Techniques; D004261:DNA Replication / Q000187:drug effects*; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D013723:Teratogens; D013997:Time Factors
D013723:Teratogens; D002699:Chlorambucil; D012313:RNA