Development of a radio-immuno-assay for oxytocin.
High affinity antibodies against oxytocin were produced by multiple intradermal injections. The antibodies do not cross react with lysine vasopressin. Reduction of the S-S link changes the immunoreactivity. The high affinity constant of the antibodies allows direct RIA of oxytocin in diluted plasma (1:5), with a sensitivity of at least 4 muU/ml. Preliminary results for oxytocin determination in human umbilical cord plasma range between 15 - 100 muU/ml.
D003429:Cross Reactions; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D006801:Humans; D007700:Kinetics; D008236:Lypressin / Q000276:immunology; D010121:Oxytocin / Q000097:blood* / Q000276:immunology; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods
D010121:Oxytocin; D008236:Lypressin