A simple radioimmunoassay for plasma cortisol and 11-deoxycortisol (17, 21-dihydroxy-4-pregnene-3, 20-dione).
A simple procedure for the measurement of cortisol and 11-deoxycortisol in plasma or serum is described. One-half ml. of plasma is extracted with methylene chloride. Separation of cortisol and deoxycortisol is achieved by a Sephadex LH-20 mini-column. The assay itself is achieved by the use of a commercially available cortisol kit employing rabbit anti-cortisol coated tubes. This antibody exhibits a 20% crossreactivity with 11-deoxycortisol. This procedure is extremely useful in the assessment of a pituitary-adrenal reserve in the Metyrapone test.
D015065:17-Hydroxycorticosteroids / Q000097:blood*; D000311:Adrenal Glands / Q000502:physiology; D000818:Animals; D003350:Cortodoxone / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D008797:Metyrapone; D010902:Pituitary Gland / Q000502:physiology; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods
D015065:17-Hydroxycorticosteroids; D003350:Cortodoxone; D006854:Hydrocortisone; D008797:Metyrapone