Plaque formation by African swine fever virus in chick embryo fibroblasts in the absence of CO2 atmosphere.
A plaque assay method using ASFV previously adapted to growth in chick embryo fibroblasts is described. Chick embryo fibroblast monolayers under bactoagar or methylcellulose have been employed using cysteine, arginine, DEAE-Dextran and HEPES as additives. Plaque production was optimal under methylcellulose. HEPES rendered the plaques more clear when used with the overlay. Arginine enhances plaque formation with bactoagar, and DEAE-Dextran doubles the plaque size. The growth curve of ASFV in chick embryo monolayers has been studied.
D000327:Adsorption; D000358:African Swine Fever Virus / Q000254:growth & development*; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002245:Carbon Dioxide; D002460:Cell Line; D002642:Chick Embryo; D003470:Culture Media; D003545:Cysteine / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D003637:DEAE-Dextran / Q000494:pharmacology; D004267:DNA Viruses / Q000254:growth & development*; D006531:HEPES / Q000494:pharmacology; D008747:Methylcellulose; D010948:Viral Plaque Assay; D014779:Virus Replication
D003470:Culture Media; D002245:Carbon Dioxide; D008747:Methylcellulose; D003637:DEAE-Dextran; D001120:Arginine; D003545:Cysteine; D006531:HEPES