[Production of anti-Aeromonas salmonicida agglutinins by the rainbow trout. Influence of temperature, an adjuvant and an immunosuppressive agent].
Adult rainbow trout were immunized intraperitoneally with a chloroform killed Aeromonas salmonicida suspension. Agglutinating antibodies were titrated using a microtiter system. Antibody activity was associated with IgM fraction obtained from Sepharose 4 B gel filtration. The kinetics of antibody formation were established at three temperatures. At 50C detectable antibodies were found at day 50 post immunization. At 10degrees C and 16 degrees C the agglutinin titer rose before day 20. The antibody titer was significantly raised by Freund's complete adjuvant, but cyclophosphamide at a total dose of 500 mg/kg of body weight administered in 10 injections before and after immunization had no effect.
D000333:Aeromonas / Q000276:immunology*; D000373:Agglutinins / Q000032:analysis*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology; D005620:Freund's Adjuvant / Q000494:pharmacology; D007075:Immunoglobulin M / Q000032:analysis; D012487:Salmonidae / Q000276:immunology*; D013696:Temperature; D014337:Trout / Q000276:immunology*
D000373:Agglutinins; D007075:Immunoglobulin M; D003520:Cyclophosphamide; D005620:Freund's Adjuvant
Production d'agglutinines anti Aeromonas salmonicida par la truite arc-en-ciel. Influence de la température, d'un adjuvant et d'un immunodépresseur.