Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus.
A factor changing Chinese hamster ovary cells from an oval to a spindle shape was isolated from the culture filtrate of Vibrio parahaemolyticus. It was partially purified by successive column chromatographies on diethylaminoethylcellulose, diethylaminoethyl-Sephadex A-25, hydroxylapatite, and Sephadex G-200. This factor was separated from thermostabl direct hemolysin and was heat labile.
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis / Q000302:isolation & purification*; D002474:Cell-Free System; D002478:Cells, Cultured; D006224:Cricetinae; D005260:Female; D005374:Filtration; D006358:Hot Temperature; D010053:Ovary / Q000166:cytology / Q000382:microbiology*; D014736:Vibrio parahaemolyticus
D001427:Bacterial Toxins