Kinetics of the light flash of the living firefly: a supramolecular process.
The light intensity vs. time curve of the light flash of the living firefly has been measured. Unlike the purified firefly enzyme system in aqueous solution, the living system does not show light decay conforming to a double exponential time curve, to simple first or second order decay, or to solid-state Elovich kinetics. Light decay of the living flash does show linearity in a probit vs. square root of time plot, which may indicate a reaction rate-limited by cooperative interactions of a biological phase transition. The observation that the kinetics of the firefly light system differ in the living cell from those in the purified system suggests that in the living system supramolecular factors control the rate of the reaction.
D000818:Animals; D001517:Coleoptera / Q000201:enzymology / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D008163:Luminescent Measurements; D008297:Male; D013997:Time Factors