[Content of the nonsaponifying substances in crystalline fructosediphosphate aldolase from the muscles of rabbits normally and in atherosclerosis].
The non-saponifying fraction was found in highly purified crystalline preparations of fructose diphosphate aldolase (EC 4.1.2.13). The amount of the non-saponifying substance in the aldolase of intact animals is different and depends not only on the degree of the enzyme purification. In the experiments when the non-saponifying residue was added to the incubation mixture it was found to produce no effect on the aldolase activity with the presence of fructose-1,6-diphosphate and fructose-1-phosphate. A decrease is observed in the activity of the crystalline preparations during their storage for four months which depends directly on the amount of the non-saponifying fraction in these preparations. The amount of the non-saponifying fraction in equally purified preparations of aldolase with experimental atherosclerosis is twice as low as compared to the norm. The non-saponifying residues of the fructose-diphosphate aldolase muscular preparations in the norm and with experimental atherosclerosis consist of two components, one of them being cholesterol, the chemical nature of the other component is not the same in the norm and with atherosclerosis.
D000818:Animals; D001161:Arteriosclerosis / Q000201:enzymology*; D002855:Chromatography, Thin Layer; D003460:Crystallization; D004789:Enzyme Activation; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis* / Q000302:isolation & purification; D008297:Male; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
D005634:Fructose-Bisphosphate Aldolase
Vmist neomyliuvanykh rechovyn u krystalichniĭ fruktozodyfosfatal'dolazi z m'iaziv kroliv u normi ĭ za aterosklerozu