Subnanogram assay for pilocarpine in biological fluids.
A method for the determination of pilocarpine was developed in which the imidazole ring of pilocarpine was acylated with heptafluorobutyric anhydride, using triethylamine as a catalyst. After cleanup, the pilocarpine derivative was analyzed using GLC with electron-capture detection. The limit of sensitivity was 25-50 pg of pilocarpine, which had been subjected to the derivatization and cleanup procedures. The method was specific for pilocarpine, with the isopilocarpine derivative eluting prior to the pilocarpine derivative.
D000818:Animals; D001082:Aqueous Humor / Q000032:analysis; D002849:Chromatography, Gas; D013058:Mass Spectrometry; D008722:Methods; D008832:Microchemistry; D010862:Pilocarpine / Q000032:analysis*; D011817:Rabbits
D010862:Pilocarpine