title: Stabilization of double-stranded DNA molecule by non-histone peptidic effector from calf thymus.
abstract: A peptidic effector from calf thymus causes a strong stabilization of DNA double-stranded molecule in vitro. The active factor was isolated from aqueous ultrafiltered thymus extracts and purified by means of chromatography on DEAE-cellulose and then on Dowex 50 WX2. The purified thymic factor was characterized as a peptide of low molecular weight (less than 5000). The biological activity of the thymic factor cannot be attributed to a histone fragment. Melting data of the control DNA and of the DNA-active factor complex in various conditions of ionic strength and dielectric constant of the solution medium are recorded.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D013949:Thymus Extracts / Q000494:pharmacology
chemical_list: D010455:Peptides; D013949:Thymus Extracts; D004247:DNA; D012321:DNA-Directed RNA Polymerases