title: [Selectivity of lysozyme oxidation by singlet oxygen].
abstract: Peptide analysis of tryptic hydrolysates of two lysozyme forms derived from oxidation of lysozyme with singlet oxygen shows that Trp-62, located at the active site, is destroyed. This is confirmed by the protective effect of the substrate (chitin), whose presense practically prevents the oxidation. A possibility of oxidating different tryptophan residues is discussed from the view-point of their availability to the reagent.
mesh_terms: D000818:Animals; D002686:Chitin / Q000494:pharmacology; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D006868:Hydrolysis; D009113:Muramidase / Q000032:analysis / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010455:Peptides / Q000032:analysis; D014364:Tryptophan / Q000032:analysis / Q000378:metabolism
chemical_list: D010455:Peptides; D002686:Chitin; D014364:Tryptophan; D009113:Muramidase; D010100:Oxygen
vernacular_title: O selektivnosti okisleniia lizotsima singletnym kislorodom.