Regulation of glucose transport in chick fibroblasts: bicarbonate, lactate and ascorbic acid.
The rate at which chick embryo fibroblasts in primary or secondary culture transport glucose or 3-O-methyl glucose is strongly influenced by the presence of bicarbonate ion in the culture medium. Cells growing or maintained on glucose at physiologic concentration (5.5 mM) have an 8 to 10 fold higher rate of glucose uptake than their counterparts cultivated without bicarbonate. These cells also produce more lactate as a consequence of their more rapid intake of glucose. The hydrogen acceptors, methylene blue and dehydroascorbate added to the culture medium reduce the cell capacity to transport glucose and 3-O-methyl glucose to levels obtaining in the bicarbonate-free medium. There is a concomitant reduction in glucose utilized by cells during 24 hours and further reduction in lactate formed per molecule of glucose metabolized.
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism*; D001693:Biological Transport, Active; D002460:Cell Line; D002642:Chick Embryo; D046508:Culture Techniques; D003683:Dehydroascorbic Acid / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D008751:Methylene Blue / Q000378:metabolism; D008757:Methylglucosides / Q000378:metabolism
D001639:Bicarbonates; D007773:Lactates; D008757:Methylglucosides; D005947:Glucose; D001205:Ascorbic Acid; D008751:Methylene Blue; D003683:Dehydroascorbic Acid