Structure of nuclear pre-mRNA. VIII. Isolation and characterization of complementary sequences.
After the annealing of pre-mRNA at high Cot values, up to 20% of the material becomes resistant to the action of ribonuclease. This has been attributed to the presence of self-complementary sequences (scRNA) in the pre-mRNA. The most important properties of scRNA, which was isolated in preparative amounts, have been studied. The approximate dimensions of the complementary segments are 45-50 nucleotides. Hybridization experiments have shown that scRNA is transcribed from repeated segments of the genome. It may be postulated that the rapidly hybridizing pre-mRNA fraction consists mainly of self-complementary sequences.
D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis*; D005796:Genes; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D012333:RNA, Messenger / Q000302:isolation & purification; D012265:Ribonucleotides / Q000032:analysis; D013696:Temperature
D012333:RNA, Messenger; D012265:Ribonucleotides