Isolation, partial characterisation of antithiamine factor present in rice-bran and its effect on TPP-transketolase system and Staphylococcus aureus.
1. A light yellow viscous oily antithiamine compound designated as Compound X was isolated from rice-bran and it was composed of three components--glucose, a yellow viscous oily substance (Fraction A) and a reddish brown amorphous substance (Fraction B). 2. 1 mg of Compound X inactivated 3.5 microgram of thiamine hydrochloride whereas 1 mg of Fraction A inactivated 26.5 microgram of thiamine hydrochloride. Fraction B had no antithiamine activity. 3. Fraction A was partially characterised as benzene nucleus with ortho dihydroxyl groups, one methyl group and a side chain containing hydroxyl and carboxylic acid groups. 4. Compound X as well as Fraction A inhibited the utilisation of ribose-5-phosphate by TPP-TK system of human erythrocyte hemolysate and the growth of Staphylococcus aureus was retarded by these compounds.
D000963:Antimetabolites / Q000302:isolation & purification* / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D012275:Oryza / Q000032:analysis*; D012267:Ribosemonophosphates / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013831:Thiamine / Q000037:antagonists & inhibitors*; D013835:Thiamine Pyrophosphate / Q000378:metabolism*; D014174:Transketolase / Q000097:blood / Q000378:metabolism*
D000963:Antimetabolites; D012267:Ribosemonophosphates; D014174:Transketolase; D013835:Thiamine Pyrophosphate; D013831:Thiamine