title: [Insulin catabolism by transformed, cultivated mouse fibroblasts (L-cells)].
abstract: Cultivated, transformed mouse fibroblasts (L-cells) are endowed with enzyme systems for insulin breakdown that are comparable to those of many other types of non-dedifferentiated insulin target cells (time and substrate dependence, Km value). It cannot yet be decided, however, which of the potential enzyme systems (thiolproteindisulfide-oxidoreductase (TPO) [E.C. 1.8.4.2] or proteinase) is prevalent in insulin breakdown in intact L-cells. Despite the lack of typical target symptoms for insulin, the cultivated L-cell could provide an appropriate experimental model for the insulin metabolism.
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D001711:Biotransformation; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D005347:Fibroblasts / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D007739:L Cells / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D011490:Protein Disulfide Reductase (Glutathione) / Q000378:metabolism; D013552:Swine
chemical_list: D007328:Insulin; D011490:Protein Disulfide Reductase (Glutathione)
vernacular_title: Abbau von Insulin durch transformierte, kultivierte Mäusefibroblasten (L-Zellen)