Polyethylene glycol-induced mammalian cell hybridization: effect of polyethylene glycol molecular weight and concentration.
The effects of polyethylene glycol (PEG) molecular weight and concentration on mammalian cell hybridization were studied. The peak hybridization-inducing activity with all grades of PEG from 400-6000 was found to occur in the concentration range of 50-55%. However, changes in concentration were seen to have different quantitative effects with different grades of PEG. For monolayer fusions, PEG 1000 at 50% seems to be the optimal combination of PEG molecular weight and concentration, in terms of both efficiency of hybridization and relative insensitivity to dilution effects.
D002459:Cell Fusion / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000494:pharmacology*
D011092:Polyethylene Glycols