[Radioautographic and cytofluorimetric analysis of DNA synthesis during the pre-implantation period of rat and mouse embryonic development].
The patterns of DNA synthesis and kinetics of cell population in the rat and mouse embryos were studied by means of 3H-thymidine autoradiography and cytofluorimetry. The rat and mouse embryos during the period of cleavage consist of a heterogenous population of blastomeres. At all the stages under study, all phases of the cell cycle occur in the blastomeres: G1, S, G2 and mitosis. The embryonic cells were distributed into groups containing 2c, 3c, 4c and more DNA. The ratio of cell number in these groups differed in the mouse and rat embryos. The mouse embryos are characterized by the appearance of a considerable amount of polyploid cells in S phase at the morula stage. The stage and species specific quantitative and qualitative patterns were established for DNA synthesis and kinetics of the cell population of blastomeres.
D000818:Animals; D001345:Autoradiography; D001755:Blastocyst / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D004622:Embryo, Mammalian / Q000378:metabolism*; D047108:Embryonic Development; D005260:Female; D005865:Gestational Age; D006651:Histocytochemistry; D051379:Mice; D008808:Mice, Inbred CBA; D011123:Polyploidy; D011247:Pregnancy; D051381:Rats; D013050:Spectrometry, Fluorescence; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D013936:Thymidine
Radioavtograficheskiĭ i tsitofluorimetricheskiĭ analiz sinteza DNK v predimplantatsionnyĭ period razvitiia zarodysheĭ krys i mysheĭ