An improved method for PHA-culturing mouse lymphocytes using Ficoll-Hypaque gradient.
A simple, reproducible method is described for in vitro culture of mouse lymphocytes purified by differential centrifugation through Ficoll-Hypaque gradient. Peripheral blood, spleen and thymus purified lymphocytes responded well to in vitro PHA stimulation as measured by blast transformation and tritiated thymidine incorporation; the lowest values were observed with thymus lymphocytes.
D000818:Animals; D000917:Antibody Formation; D002478:Cells, Cultured; D002498:Centrifugation / Q000379:methods; D037102:Lectins; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D037102:Lectins