title: [Posthypoxic changes in the cerebral cortex of dogs in the late recovery period after 4-hour hypovolemic hypotension].
abstract: On the 14th-21st day of the restorative period after four-hour hypovolemic hypotension the level of total RNA decreased in the tissue of the gray matter of the brain by 20.9%, and of DNA-by 13%. In the postmitochondrial supernatant the concentration of prealbumins was reduced by 26.5%, alpha-globulins--19.2%, gamma-globulins--by 59.8%; the concentration of albumins and beta-globulins was increased by 12.6% and 50.0%, respectively. The activity of acid cathepsins  rose by 50%, and of acid phosphatase--by 44%. The activity of total lactic dehydrogenase (LDH) and glutamic dehydrogenase failed to differ essentially from the control level. However, LDH isoenzyme spectrum changes towards the reduction of LDH3+4+5 from 31.9 to 14.2%. Analysis of densitograms of electrophoresis in polyacrylamide gel showed physico-chemical changes in the protein molecules similar in nature to the denaturation phenomenon. The Purkinje's cell count decreased in the cerebellum by 41.3% in comparison with control.
mesh_terms: D000818:Animals; D001810:Blood Volume; D002540:Cerebral Cortex / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004285:Dogs; D005260:Female; D007022:Hypotension / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D011489:Protein Denaturation; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012151:Resuscitation; D013997:Time Factors
chemical_list: D011506:Proteins; D012313:RNA; D004247:DNA
vernacular_title: Postgipoksicheskie izmeneniia v kore golovnogo mozga u sobak v otdalennyĭ vosstanovitel'nyĭ period posle 4-chasovoĭ gipovolemicheskoĭ gipotenzii