Current status of the thiol redox model for the regulation of hexose transport by insulin.
Data obtained over the last two years pertinent to the thiol redox model for the modulation of hexose transport activity by insulin is summarized. The model proposes that activation of hexose transport in fat cells involves sulfhydryl oxidation to the disulfide form in a key protein component of the fat cell surface membrane. Theoretically, the rapid activation of transport by insulin may involve either the conversion of inactive membrane carriers to the active form as originally proposed, or the conversion of a low Vmax transport system to a high Vmax form. The present experiments showed that the percent inhibition of insulin-activated transport rates by submaximal levels of cytochalasin B was decreased compared to its effects on basal transport. Treatment of fat cells with N-ethylmaleimide inhibited cytochalasin B action but not transport activity. When insulin or the oxidant vitamin K5 was added to cells 5 minutes before the N-ethylmaleimide, the elevated transport activity was also resistant to the sulfhydryl reagent, but cytochalasin B retained its potent inhibitory effect on transport. The data demonstrate that unique properties characterize basal versus insulin-activated transport activity with respect to the sensitivity of cytochalasin B action to sulfhydryl blockade in isolated fat cells. The data are consistent with the concept that activation of transport activity reflects the conversion of a reduced (sulfhydryl) system characterized by a low Vmax to an oxidized (disulfide), high Vmax transport system.
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D006601:Hexoses / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D008757:Methylglucosides / Q000378:metabolism; D008954:Models, Biological; D051381:Rats; D011972:Receptor, Insulin / Q000187:drug effects; D014812:Vitamin K / Q000494:pharmacology
D006601:Hexoses; D007328:Insulin; D008757:Methylglucosides; D014812:Vitamin K; D003571:Cytochalasin B; D011972:Receptor, Insulin; D005033:Ethylmaleimide