Disruption of the metabolism, motility and morphology of spermatozoa by injection of alpha-chlorohydrin into rams.
Ejaculated spermatozoa from rams given intramuscular injections of alpha-chlorohydrin (25 mg/kg, daily for 5 days) were studied. Respiratory and glycolytic activity of the spermatozoa was almost entirely suppressed within 1 day and motility had decreased within 4 days of the first injection. Morphologically abnormal spermatozoa appeared in ejaculates after 2 weeks. The most common abnormality was an increase in the number of spermatozoa with looped or bent tails. There was little change in the fructose or amino acid concentration of the seminal plasma. All effects of alpha-chlorohydrin were fully reversible. It is suggested that the initial primary mode of action of alpha-chlorohydrin is to disrupt the metabolism of spermatozoa in the cauda epididymis.
D000818:Animals; D002728:Chlorohydrins / Q000494:pharmacology*; D003864:Depression, Chemical; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D012661:Semen / Q000032:analysis; D012756:Sheep / Q000502:physiology*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000517:alpha-Chlorohydrin / Q000494:pharmacology*
D002728:Chlorohydrins; D005632:Fructose; D000517:alpha-Chlorohydrin; D005947:Glucose