Interferon synthesis in mouse peritoneal cells damaged by X-irradiation.
NDV-induced interferon of peritoneal cells of irradiated (X-rays, 400 R) and control mice was investigated in vitro. Irradiation or treatment with hydroxyurea (10(-5) M) and mitomycin C (25 microng/ml) did not change interferon synthesis in spite of an 80--90% inhibition of 3H-thymidine incorporation. Increased doses of mitomycin C and treatment with actinomycin D and puromycin blocked interferon production. De novo interferon synthesis occurred in cells with damaged replicative activity of DNA caused by irradiation or by treatment with antimetabolites.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology* / Q000528:radiation effects; D002452:Cell Count; D002478:Cells, Cultured / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D006918:Hydroxyurea / Q000494:pharmacology; D007372:Interferons / Q000096:biosynthesis*; D051379:Mice; D008937:Mitomycins / Q000494:pharmacology; D009522:Newcastle disease virus; D011691:Puromycin / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014965:X-Rays
D000596:Amino Acids; D008937:Mitomycins; D003609:Dactinomycin; D011691:Puromycin; D007372:Interferons; D013936:Thymidine; D014529:Uridine; D006918:Hydroxyurea