Proteins of animal ribosomes. XXV. Proteins of rat liver ribosomes protected by polyuridylic acid from methyl acetimidate substitution.
Addition of poly(U) to complexes of 40S and 60S subunits of rat liver ribosomes decreases the substitution of amino groups of 12 proteins of the small ribosomal subunit and of 11 proteins of the large subunit by [14C]-methyl acetimidate. When comparing the results obtained with this amino group specific reagent with the reactivity of the proteins against iodoacetamide it becomes obvious that 4 proteins of the small ribosomal subunit (S12, 18, 19, 24) and 3 proteins of the large one (L20, 22, 25) are partially protected by poly(U) against reaction with both reagents.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D007094:Imides; D007096:Imidoesters; D007202:Indicators and Reagents; D008099:Liver / Q000378:metabolism; D011072:Poly U / Q000494:pharmacology*; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D051381:Rats; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D007094:Imides; D007096:Imidoesters; D007202:Indicators and Reagents; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D011072:Poly U; C010222:methyl acetimidate