[Comparative study of 21S RNP particles and ribosomes from the cytoplasm of the cells of sarcoma M-1 in vitro].
Preparations of RNP-particles from rat sarcoma M-1 were obtained by means of treatment with different detergents: anione - sodium deoxycholate (1%) and non-ionic - Triton X-100 (1%). Triton X-100 caused some increase in content of the small ribosomal subunit and also in the content of 21 S and 7.5 S particles in the preparation. The latter particles as well as ribosomes were resistant to different detergents. 21 S RNP-particles were stable in 1 M KCl. In a cell-free system 21 S and 7.5 S RNP-particles lost a significant amount of labelled protein, which appeared partially in the ribosomes.
D000818:Animals; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D003902:Detergents; D066298:In Vitro Techniques; D007930:Leucine; D009698:Nucleoproteins / Q000032:analysis*; D009994:Osmolar Concentration; D011189:Potassium Chloride; D051381:Rats; D012261:Ribonucleoproteins / Q000032:analysis* / Q000302:isolation & purification; D012270:Ribosomes / Q000032:analysis*; D012513:Sarcoma, Experimental / Q000473:pathology*
D002250:Carbon Radioisotopes; D003902:Detergents; D009698:Nucleoproteins; D012261:Ribonucleoproteins; D011189:Potassium Chloride; D007930:Leucine
Sravnitel'noe izuchenie 21S RNP-chastits i ribosom tsitoplazmy kletok sarkomy M-1 in vitro