Metabolic effect of alpha-and the beta-adrenergic stimulation of rat submaxillary gland in vitro.
1. Incubation of submaxillary-gland slices with isoproterenol, a beta-adrenergic agonist, stimulated glucose removal by 41% and decreased tissue [glucose 6-phosphate] by 50%. Propranolol blocked these effects of isoproterenol. 2. Phenylephrine, an alpha-adrenergic agonist, stimulated glucose removal by 35% and decreased tissue [glucose 6-phosphate] by 75%. In addition, phenylephrine also completely overcame the inhibition of pyruvate removal caused by acetoacetate metabolism and decreased tissue [atp] by 45%. Phentolamine blocked the effects of phenylephrine. 3. In contrast with beta-adrenergic stimulation, alpha-adrenergic stimulation required exogenous Ca2+. 4. These results explain the different metabolic responses of the submaxillary gland to adrenaline in the presence and absence of exogenous Ca2+.
D000090:Acetoacetates / Q000378:metabolism; D000818:Animals; D005947:Glucose / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D007773:Lactates / Q000378:metabolism; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology*; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D013268:Stimulation, Chemical; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*; D013565:Sympatholytics / Q000494:pharmacology
D000090:Acetoacetates; D007773:Lactates; D011773:Pyruvates; D013565:Sympatholytics; D010656:Phenylephrine; D005947:Glucose; D007545:Isoproterenol