title: [Bacteriophage T2 DNA modification by O-methylhydroxylamine].
abstract: A comparative study of the reactivities of free 5-hydroxymethylcytosine (5-HMC) and 5-HMC found in the composition of native, denaturated and intraphage DNA of the T2 phage with that of O-methylhydroxylamine (OMHA) demonstrated that the DNA secondary structure in situ is partially disturbed. The interaction DNA-protein in the phage particle channels the reaction into a predominant formation of 4N-methoxy-6-methoxyamino-5,6-dihydro-5-hydroxymethyl cytosine, but not 4N-methoxy-5-hydroxymethyl cytosine, which is formed in vitro. In the course of the reaction the interaction DNA-protein is probably fixed by covalent binding.
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D055598:Chemical Phenomena; D002621:Chemistry; D003090:Coliphages; D003596:Cytosine / Q000031:analogs & derivatives; D004279:DNA, Viral / Q000378:metabolism; D006898:Hydroxylamines; D009691:Nucleic Acid Denaturation; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D006898:Hydroxylamines; D014764:Viral Proteins; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine
vernacular_title: Modifikatsiia O-metilgidroksilaminom DNK bakteriofaga T2