[Study of nucleic acid synthesis in rat embryos in relation to phase-specific characteristics of teratogenic effect of chloridine].
The maximal rate of incorporation of 32P-phosphate, 14C-formate and 14C-thymidine in DNA was recorded on the 13th day of development in the rat embryos and that of 14C-formate and 32P-phosphate in RNA and nucleotides of the acid-soluble fraction on the 12th day. The maximal incorporation of 14C-formate was recorded later: on the 15-16th days. Chloridin inhibited the incorporation of 14C-formate in DNA at all developmental stages, irrespective of the sensitivity of embryos to its teratogenic effect. The period of the maximal rate of DNA synthesis coincides with that of highest teratogenic activity of the drug. A suggestion is put forward to the effect that quantitative differences in the intensity of DNA synthesis at different stages of organgenesis provide one of the main causes of differential sensitivity of embryos to the teratogenic effect of inhibitors of nucleic acid synthesis.
D000818:Animals; D002250:Carbon Radioisotopes; D004247:DNA / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D007553:Isotope Labeling; D008958:Models, Molecular; D009024:Morphogenesis / Q000187:drug effects; D010761:Phosphorus Radioisotopes; D011247:Pregnancy; D011739:Pyrimethamine / Q000494:pharmacology*; D012313:RNA / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051381:Rats; D013723:Teratogens / Q000494:pharmacology*
D002250:Carbon Radioisotopes; D010761:Phosphorus Radioisotopes; D013723:Teratogens; D012313:RNA; D004247:DNA; D011739:Pyrimethamine
Izuchenie sinteza nukleinovykh kislot v zarodyshakh krys v sviazi so stadiospetsificheskimi osobennostiiami teratogennykh effektov khloridina