Proteins of chick duodenal brush borders during developmental changes.
Duodenal brush border membrane proteins were studied in chicks at different developmental stages. The protein pattern obtained from polyacrylamide gels with 2-day-old chick preparations was distinctly different from that obtained with 20-day embryos. The most remarkable changes were seen in the region of a protein with an Rf of 0.25, an area with high sucrase and maltase maltase activity, and in the region of a protein with an Rf of 0.28, which was characterized by alkaline phosphatase activity. These proteins reacted strongly with carbohydrate stain after hatching.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000502:physiology*; D004386:Duodenum / Q000196:embryology / Q000502:physiology*; D005959:Glucosidases / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D013393:Sucrase / Q000378:metabolism
D008565:Membrane Proteins; D000469:Alkaline Phosphatase; D005959:Glucosidases; D013393:Sucrase; D007931:Leucyl Aminopeptidase