title: Activity and intracellular localization of lysosomal acid phosphatase in lymphocytes from patients with Hodgkin's disease, plasma cell myeloma and primary polycythemia.
abstract: Using the cytochemical method after Barka and Anderson, activity and localization of lysosomal acid phosphatase (AP) was determined in peripheral-blood lymphocytes from 20 healthy subjects, 10 patients with Hodgkin's disease (HD), 10 patients with plasma cell myeloma (PCM), and 10 patients with primary polycythemia (PP). Total count of AP-positive lymphocytes was lowest in the patients with HD. Moreover, they showed a significant decrease of the absolute count of lymphocytes with well formed and more numerous AP-positive lysosomal granules. Analogous alterations in lymphocytes from patients with PCM and those with PP were much less significant. The authors discuss the importance of the above-mentioned observations for evaluation on a cellular basis of lowered immunity of patients with lympho-proliferative and myelo-proliferative disorders, with special regard to AP as a T-cell marker.
mesh_terms: D000135:Acid Phosphatase / Q000097:blood*; D001772:Blood Cell Count; D004796:Clinical Enzyme Tests; D006689:Hodgkin Disease / Q000097:blood / Q000201:enzymology*; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D009101:Multiple Myeloma / Q000097:blood / Q000201:enzymology*; D011086:Polycythemia / Q000097:blood / Q000201:enzymology*
chemical_list: D000135:Acid Phosphatase