[Transcription of repeated and unique DNA nucleotide sequences in rat thymus cells stimulated with phytohemagglutinin].
Transcription of unique and repeated DNA sequences in normal and phytohemagglutinin (PHA)-stimulated rat thymus cells is studied. It is found that heterogenous nuclear RNA (hnRNA) contains the transcript both from unique and repeat-d DNA sequences. PHA does not induce re-distribution of hnRNA fractions transcripted from different for their recurrency genome regions. Moreover, hnRNA from normal thymus cells competes with RNA from stimulated cells within the range of COt values (10(-3)-10(-4)) in which the hybridization has been carried out. It is concluded that no activation of new genome regions takes place in thymocytes.
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D005838:Genotype; D037102:Lectins / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D012313:RNA; D051381:Rats; D012265:Ribonucleotides / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic
D037102:Lectins; D012265:Ribonucleotides; D012313:RNA; D004247:DNA
Transkriptsiia povtoriaiushchikhsia i unikal'nykh posledovatel'nosteĭ DNK kletok timusa krbsy, stimulirovannykh fitogemaggliutininom