Isolation of 4-ribitylamino-5-amino-2,6-dihydroxypyrimidine from a high flavinogenic mold Eremothecium ashbyii1.
The addition of glyoxal, a trapping agent, caused simultaneously the accumulation of a green fluorescent compound and the inhibition of riboflavin formation in non-growing cells of Eremothecium ashbyii. The fluorescent compound purified was identified as 8-ribityllumazine from the results of spectrophotometric and fluorometric analyses. Accordingly, the fragment, except for the glyoxal portion on the 8-ribityllumazine molecule, 4-ribitylamino-5-amino-2,6-dihydroxypyrimidine, is believed to be an intermediate in the riboflavin biosynthetic pathway.
D001203:Ascomycota / Q000378:metabolism*; D006037:Glyoxal / Q000494:pharmacology; D011743:Pyrimidines / Q000302:isolation & purification*; D012256:Riboflavin / Q000096:biosynthesis; D012266:Ribose / Q000031:analogs & derivatives* / Q000302:isolation & purification; D004718:Saccharomycetales / Q000378:metabolism*
D011743:Pyrimidines; D006037:Glyoxal; D012266:Ribose; D012256:Riboflavin