Phagocytosis of concanavalin A in normal and enucleated cultures of mammalian cells.
The fate of concanavalin A (Con A) bound to normal and enucleated L cells was followed at the ultrastructural level over a 20-h period. In both enucleates and normal cells the Con A is seen to be distributed in a uniform manner over the entire cell surface following a 30-min pulse with a low concentration of Con A. In the subsequent chase period the cells then aggregate the Con A and Con A sites into large clusters on the cell membrane. The cells then phagocytoze the Con A and large phagocytic vacuoles containing it are observed. Thus, enucleated cells are capable of phagocytozing Con A and its sites in the same manner as normal cells.
D002458:Cell Fractionation; D002467:Cell Nucleus; D003208:Concanavalin A; D007739:L Cells / Q000502:physiology* / Q000648:ultrastructure; D010587:Phagocytosis; D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D014617:Vacuoles / Q000648:ultrastructure
D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D003208:Concanavalin A