[An enzymic assay for the determination of vitamin B12 (author's transl)].
A procedure which extends the very sensitive propanediol-dehydratase reaction for 5'-deoxy-5'-adenosylcobalamine determination to cyanocobalamine and hydroxocobalamine is described. In this procedure, the cobalamines are adenosylated by vitamin B12 coenzyme synthetase and the resulting 5'-deoxy-5'-adenosylcobalamine can be determined by the propanediol-dehydratase reaction. Aldehyde, the product of the latter, can be registered by the alcohol dehydrogenase reaction.
D000241:Adenosine; D000429:Alcohol Oxidoreductases; D000447:Aldehydes / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D008722:Methods; D011408:Propanediol Dehydratase; D014166:Transferases; D014805:Vitamin B 12 / Q000032:analysis*
D000447:Aldehydes; D000429:Alcohol Oxidoreductases; D014166:Transferases; D011408:Propanediol Dehydratase; D000241:Adenosine; D014805:Vitamin B 12
Enzymatische Vitamin B12-Bestimmung