[Changes in the surface structures of bacteria as a cause of weak immunogenicity of vaccines from several strains of Citrobacter].
The authors present the results of studying the weakly immunogenic Citrobacter strains; some O-antigens of Citrobacter (11, 14, 19, 21a, 21b, 26) caused in immunization of rabbits a weak immune response (antibody titre--1:400-1:800). An electron microscopic study of the vaccines (obtained from these strains) and their fragments showed that a partial desquamation and denaturing of the antigenic material occurred in the process of preparation of heated vaccines. Formalin-treated vaccine subjected to preliminary deflaggelation for 2 min at 3000 cps was used for obtaining the O-sera against the mentioned antigens.
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines / Q000592:standards*; D002458:Cell Fractionation; D002954:Citrobacter / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure; D004755:Enterobacteriaceae / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology*; D005557:Formaldehyde / Q000494:pharmacology; D006358:Hot Temperature; D011817:Rabbits; D014611:Vaccination
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D005557:Formaldehyde
Izmenenie poverkhnostnykh struktur bakteriĭ kak prichina slaboĭ immunogennosti vaktsin iz nekotorykh shtammov Citrobacter