Identification of 6-ketoprostaglandin F1alpha formed from arachidonic acid in bovine seminal vesicles.
The prostaglandin synthesizing system in bovine seminal vesicles was characterized by a radiometric assay. Two main products were formed from [1-14C]-arachidonic acid, and their structures were confirmed by mass spectrometry. The less polar product was identical with prostaglandin E2 and the more polar one was identical with a new prostaglandin, i.e., 6-ketoprostaglandin F1alpha.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002417:Cattle; D002849:Chromatography, Gas; D008297:Male; D008861:Microsomes / Q000378:metabolism; D011460:Prostaglandins F / Q000096:biosynthesis* / Q000302:isolation & purification; D012669:Seminal Vesicles / Q000378:metabolism*; D013057:Spectrum Analysis
D001095:Arachidonic Acids; D011460:Prostaglandins F