[Effects of eserine and ouabain on the febrile reaction induced by lipopolysaccharide].
We investigated the effects of eserine and ouabain on the permeability of the blood-brain barrier (B.B.B.) as related to the febrile response induced with LPS in rabbit. Results are as follows; The febrile response induced by LPS (0.02 and 1.0 mug/kg) i.v. was suppressed by administration of ouabain (0.06 mg/kg, i.v.). Contrary to the febrile response of LPS given i.c. (10(-4) and 10(-3) mug/kg), the febrile response was not suppressed with the same dose of ouabain. The pyrogenicity of cerebrospinal fluid (CSF) withdrawn at two hours after rabbits had been injected with LPS (25 mug/kg) was suppressed by ouabain (0.06 mg/kg, i.v.). Pyrogenic response was enhanced by pretreatment with eserine (0.5 mg/kg, s.c.) given one hr before LPS (1 mug/kg, i.v.). The pyrogenicity of CSF was also potentiated to a greater extent by pretreatment of eserine than with LPS alone. In the eserinized rabbit (0.5 mg/kg, s.c.), the pyrogenicity of CSF was potentiated to a greater extent by ACh (10 mug/kg, i.v.) than by LPS (1 mug/kg, i.v.) alone. From these data, it is concluded that the inhibition of Na, K-ATPase by ouabain decreases the pyrogenicity of LPS, while the inhibition of cholinesterase by eserine enhances the pyrogenicity.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D001831:Body Temperature / Q000187:drug effects; D005334:Fever / Q000134:cerebrospinal fluid / Q000139:chemically induced*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D008297:Male; D010042:Ouabain / Q000494:pharmacology*; D010830:Physostigmine / Q000494:pharmacology*; D011135:Polysaccharides, Bacterial / Q000037:antagonists & inhibitors; D011749:Pyrogens / Q000037:antagonists & inhibitors; D011817:Rabbits
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D011749:Pyrogens; D010042:Ouabain; D010830:Physostigmine; D000109:Acetylcholine