title: Effect of sodium fluoride on glycolysis in human erythrocytes and Ehrlich ascites tumour cells in vitro.
abstract: In human erythrocytes and Ehrlich ascites tumour cells, NaF inhibites aerobic glucose utilization and lactate formation. The inhibition of glycolysis was accompanied by a decrease in cellular pyruvate and ATP, and by accumulation of 2-hosphoenolpyruvate. These results and direct enzymatic determinations showed that fluoride inhibits, in addition to enolase (phosphopyruvate hydratase, EC 4.2.1.11), also pyruvate kinase.
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002460:Cell Line; D004912:Erythrocytes / Q000378:metabolism*; D005459:Fluorides / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects*; D006801:Humans; D010751:Phosphopyruvate Hydratase / Q000037:antagonists & inhibitors; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors; D012969:Sodium Fluoride / Q000494:pharmacology*
chemical_list: D012969:Sodium Fluoride; D011770:Pyruvate Kinase; D010751:Phosphopyruvate Hydratase; D005459:Fluorides