[Pharmacocinetic behaviour of alpha-L-arabinofuranosidase in therapeutical application to toxify transport forms of cancerostatic substances (author's transl)].
The present studies were carried out to obtain pharmacokinetic data about the elemination of intravenously administered alpha-Larabinofuranosidase from the blood stream and about the distribution and retention of the enzyme in different organs and in a transplantable sarcoma of mouse. The results were used for the planning of therapeutical experiments, in which detoxified transport forms of cancerostatic agents were to be toxified by exogenous enzymes specifically in the tumour tissue. The alpha-Larabinofuranosidase is eliminated from the blood circulation quite rapidly (half-life about 20 min); a large portion is apparently excreted through the kidneys. The distribution in the organs, as compared with the tumour tissue, is very favourable for the planned therapeutical experiments in relation to other enzymes: The enzyme is scarcely accumulated in the RES-containing organs. In the tumour tissue, the enzymatic activity declines linerly with time of the experiment, while elimination of the enzyme from the kidney and muscle follows rather an exponential function. The optimal time interval between the enzyme and substate injections was determined to be 6 hrs.
D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism*; D001089:Arabinose; D001234:Aspergillus niger / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D012509:Sarcoma / Q000378:metabolism; D013997:Time Factors
D000970:Antineoplastic Agents; D001089:Arabinose; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase
Uber das pharmakokinetische Verhalten der alpha-L-Arabinofuranosidase bei der therapeutischen Anwendung zur Giftung von Transportformen Kanzerostatischer Stoffe