Characterization of poly(A)-protein complexes isolated from free and membrane-bound polyribosomes of Ehrlich ascites tumor cells.
Proteins present in messenger ribonucleoprotein particles were labeled with [35S]-methionine in Ehrlich ascites tumor cells in which synthesis of new ribosomes was inhibited. Poly(A)-protein complexes were isolated from free and membrane-bound polyribosomes by sucrose gradient centrifugation and affinity chromatography on oligo(dT)-cellulose. Both classes of Poly(A)-protein particles contain a poly(A) chain of about 70 adenyl residues and a protein with a molecular weight of 76000 attached to it.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis; D008566:Membranes / Q000032:analysis; D011061:Poly A / Q000032:analysis*; D011132:Polyribosomes / Q000032:analysis*; D012269:Ribosomal Proteins / Q000032:analysis*
D012269:Ribosomal Proteins; D011061:Poly A