[Distribution of mineralocorticoid receptors in the kidney cells of rats with different levels of aldosterone in their bodies].
A metabolic method was applied to the study of the distribution of mineralocorticoid receptors in the kidney cells of rats at various aldosterone levels in the organism. A great number of binding sites of aldosterone in the cytoplasm and a low aldosterone-H3 binding by the nuclei of kidney cells was revealed in adrenalectomized rats, as well as in the animals kept under conditions of sodium load, when endogenous aldosterone was absent in the organism. A reduction of the number of binding sites in the cytoplasm and an increase of aldosterone-H3 binding in the nuclei was noted in the animals with a high content of the given hormone in the organism (sodium deficiency in food or aldosterone administration to the adrenalectomized animals). The difference in the hormone binding by the nuclei of the kidney cells of rats kept under conditions of sodium loading or deficiency corresponded to an even greater difference in the aldosterone binding by the chromatin proteins of such nuclei. It is supposed that an increased aldosterone concentration in the organism intensified the change from the cytoplasm and the accumulation of specific aldosterone-receptor complexes in the nuclei.
D000315:Adrenalectomy; D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D066298:In Vitro Techniques; D007553:Isotope Labeling; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D011987:Receptors, Steroid; D012964:Sodium / Q000172:deficiency; D014316:Tritium
D011987:Receptors, Steroid; D014316:Tritium; D000450:Aldosterone; D012964:Sodium
Raspredelenie mineralokortikoidnykh retseptorov v kletkakh pochek krys pri razlichnykh urovniiakh soderzhaniia al'dosterona