title: Release of RNA--DNA-protein complex during differentiation of the water mould Allomyces arbuscula.
abstract: A correlation between release of a nucleic acid protein complex into the external medium and sporangial differentiation was found. Conditions preventing differentiation also stopped the release. Lethal lysis is not involved in this release process. Extracellular nucleic acids are very heterogenous, consisting of nucleotides as well as acid-precipitable nucleic acids. RNA was found to be associated with proteins and a hetero-duplex RNA--DNA associated with this nucleoprotein. Some speculations are presented about possible correlation between the release of nucleoprotein complexes and the intracellular events of differentiation.
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D008411:Chytridiomycota / Q000166:cytology* / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000032:analysis; D003609:Dactinomycin / Q000494:pharmacology; D003851:Deoxyribonucleases; D003852:Deoxyribonucleoproteins / Q000032:analysis / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D005658:Fungi / Q000166:cytology*; D009698:Nucleoproteins / Q000032:analysis / Q000378:metabolism*; D011370:Proflavine / Q000494:pharmacology; D012313:RNA / Q000032:analysis; D012260:Ribonucleases; D012261:Ribonucleoproteins / Q000032:analysis / Q000378:metabolism
chemical_list: D003852:Deoxyribonucleoproteins; D005656:Fungal Proteins; D009698:Nucleoproteins; D012261:Ribonucleoproteins; D003609:Dactinomycin; D012313:RNA; D004247:DNA; D003513:Cycloheximide; D011370:Proflavine; D003851:Deoxyribonucleases; D012260:Ribonucleases