title: Changes in plasma volume and haematocrit in intact and splenectomized sheep during feeding.
abstract: Changes in jugular haematocrit during daily 2-h feeding periods in trained sheep with and without spleens were compared with changes in the concentration of the plasma tracer radio-iodinated human serum albumin. Jugular haematocrit was increased by 16% in intact sheep and 9% in splenectomized sheep 20 min after they started to eat dry rations. The dilution of tracer in plasma, studied after mixing in the vascular system had been completed, showed four phases. Phase 1 was the rate of removal of tracer in the period before eating began. Phase 2 was the sudden increase in plasma radioactivity that occurred in the first 20 min of eating, indicating a loss of plasma from the circulation. Phase 3 was the decrease in tracer concentration during the remaining feeding period and phase 4 was a post-feeding phase, characterized by a slower rate of decrease of tracer than during phase 3, implying that there was significant recycling of tracer during this phase. The sudden increase in plasma radioactivity, initiated by the onset of feeding, represented a reduction in plasma volume of 10-12%. Minimum plasma volume coincided with peak haematocrit values. The reduced plasma volume accounted for the increased haematocrit in splenectomized sheep, but only accounted for about half of the increase in intact sheep. The residual increase in haematocrit in intact sheep was most likely the result of splenic contraction.
mesh_terms: D000818:Animals; D004435:Eating; D005215:Fasting; D006400:Hematocrit; D010953:Plasma Volume; D012711:Serum Albumin, Radio-Iodinated; D012756:Sheep / Q000097:blood / Q000502:physiology*; D013154:Spleen / Q000502:physiology*; D013156:Splenectomy
chemical_list: D012711:Serum Albumin, Radio-Iodinated