Separation and estimation of short-chain fatty acids and organic acids by thermodetective liquid chromatography.
Short-chain fatty acids and organic acids in aqueous solution were separated quantitatively by using a liquid chromatography. JLC-2A, which posseses a thermodetector with ion-exchange resin. A peak height of thermogram showing a thermal difference caused by adsorption and desorption of substance was proportional to the concentration. Each sample was detectable at least between approximately 10(-5) and 2 X 10(-3) moles. The "inter-day" coefficient of variation obtained from two peak heights of acetic and n-butyric acids was 1.9 and 2.3%, respectively.
D000085:Acetates / Q000032:analysis; D000327:Adsorption; D002087:Butyrates / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D003437:Crotonates / Q000032:analysis; D005232:Fatty Acids, Volatile / Q000032:analysis*; D006358:Hot Temperature; D008314:Malonates / Q000032:analysis; D008798:Mevalonic Acid / Q000032:analysis*
D000085:Acetates; D002087:Butyrates; D003437:Crotonates; D005232:Fatty Acids, Volatile; D008314:Malonates; D008798:Mevalonic Acid