O-(Carboxymethyl)oxime steroidal haptens linked in the C3 position: study of the characteristics of antibodies against 17alpha-hydroxyprogesterone and testosterone and of their evolution with time.
The production of highly sensitive and specific antisera against 17alpha-hydroxyprogesterone (17 OHP) is reported. 17 OHP was rendered antigenic by covalent linkage to bovine serum albumin through position 3 of the steroid. An unambiguous method to prepare the mono-3-0-(carboxy-methyl) oxime derivative (CMO) of 17 OHP is described starting from 17 OHP-acetate. Antisera raised in rabbits were of high affinity for 17 OHP (Ka = 1 to 2 x 10(10) L/mole) and showed very little (less than 0.7%) or no cross reaction with a variety of steroids. Cross-reaction with 17alpha-hydroxy pregnenolone was however significant. When studied in relation to time, cross-reaction of the latter steroid decreased significantly (18 to 2%) while Ka remained at the same level. The same study of the evolution with time (up to 600 days) of the characteristics of antisera raised against the 3 CMO derivative of testosterone is also reported.
D000818:Animals; D000906:Antibodies; D003429:Cross Reactions; D006241:Haptens; D006908:Hydroxyprogesterones / Q000032:analysis* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D010091:Oximes; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D012710:Serum Albumin, Bovine; D013739:Testosterone / Q000032:analysis* / Q000276:immunology; D013997:Time Factors
D000906:Antibodies; D006241:Haptens; D006908:Hydroxyprogesterones; D010091:Oximes; D012710:Serum Albumin, Bovine; D013739:Testosterone