Metabolic consequences of Sephadex-induced reduction of coronary flow in isolated rat heart.
With a polysaccharide microsphere suspension (Sephadex), coronary flow of isolated perfused rat hearts was reduced by approximately 70%. During this Sephadex-induced ischemia, the energy charge and creatine phosphate content of the myocardial tissue dropped significantly, while total nucleoside and lactate release from the heart increased. During hypoxia (30% O2), changes in high-energy phosphate content and lactate and nucleoside release were similar to the changes induced by ischemia. During hypoxia, coronary flow rate was increased by 47%. Thus, Sephadex-induced reduction of coronary flow could be a useful model in studies of metabolic change during ischemia.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects*; D003327:Coronary Disease / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D000860:Hypoxia / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D011684:Purine Nucleosides / Q000378:metabolism; D051381:Rats; D012685:Sepharose / Q000494:pharmacology*
D000227:Adenine Nucleotides; D007773:Lactates; D011134:Polysaccharides; D011684:Purine Nucleosides; D010725:Phosphocreatine; D012685:Sepharose