title: The DNA of Volvox carteri: a biophysical and biosynthetic characterization.
abstract: Various biophysical and biosynthetic characteristics of deoxyribonucleic acid (DNA) from Volvox carteri are examined. The DNA from three strains (HK-10,NB-7 and KA-1) is compared, and all strains are shown to contain at least two distinct DNA species which band at densities of 1.714-1.715 and 1.704-1.705 g/cm3 in neutral CsCl and correspond to nuclear and "cytoplasmic" DNA, respectively. Base compositions calculated from these densities, 55-56% G+C for nuclear DNA, and 45-46% G+C for cytoplasmic DNA, are in close agreement with % G+C values estimated from thermal denaturation data. DNA from strain KA-1 has a third component with a buoyant density of 1.693 g/cm3. DNA synthesis is analysed using radioactively labelled heterogeneously grown strains of Volvox carteri and profiles obtained following preparative CsCl density gradient centrifugation are presented. In addition, dissimilarities in patterns of DNA synthesis at various periods in the asexual life cycle are reported for synchronous cultures of strain HK-10. These differences in temporal patterns of DNA synthesis clearly indicate that while nuclear DNA is make to some degree throughout the life cycle, cytoplasmic DNA synthesis appears to occur only at discrete intervals.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D056890:Eukaryota / Q000254:growth & development / Q000378:metabolism*; D006147:Guanine / Q000032:analysis; D009691:Nucleic Acid Denaturation
chemical_list: D006147:Guanine; D003596:Cytosine; D004247:DNA