[Determination of glucose-6-phosphate dehydrogenase activity in cattle and swine erythrocytes using the optical test under optimal measuring conditions].
To find out optimal measuring conditions for determination of G-6-PDH-activity in erythrocytes of cattle and pigs by means of the optical test the dependence from nature, concentration and pH of buffer surroundings as well as from concentration of substrate (G-6-P), coenzyme (NADP) and activator (Mg++) was studied. From the results obtained, best reaction conditions for determination of G-6-PDH-activity in this species exist taking the following composition of the measuring sample (final concentration): 120 mM TRIS/HCl-buffer, pH 9.0; 10 mM MgSO4; 2 mM G-6-P; 0.2 mM NADP and 0.1 ml haemolysate in 3.0 ml of the measuring volume. The influence of an optimal planning of the reaction conditions on registration of G-6-PDH-activity in erythrocytes of the farming animals is discussed.
D000818:Animals; D002417:Cattle / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D008722:Methods; D013552:Swine / Q000097:blood*
D005954:Glucosephosphate Dehydrogenase
Bestimmung der Glukose-6-phosphat-Dehydrogenase-Aktivität in den Erythrozyten von Rind und Schwein mit dem optischen Test unter optimalen Messbedingungen