The suppressive effect of halothane on DNA synthesis of immunocytes.
Mice, previously immunized with sheep erythrocytes, were anaesthetized with halothane and the splenic lymphoid cells were incubated with 3H-thymidine at 1-h intervals. Abiphasic response was observed; 0.9 and 1.5% halothane reduced 3H-thymidine incorporation into the primed lymphoid cells by 83 and 94% respectively, while 0.4% halothane stimulated it by 5% of control at 3H. Immunosuppression by halothane may be the result of reduced 3H-thymidine uptake of the dividing immunocytes.
D000818:Animals; D000921:Antibody-Producing Cells / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D006221:Halothane / Q000494:pharmacology*; D007165:Immunosuppression Therapy; D066298:In Vitro Techniques; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000276:immunology
D004247:DNA; D006221:Halothane