The large-scale cultivation of diploid cell strains in microcarrier culture. Improvement of microcarriers.
The use of diploid cell strains for virus vaccine production is mostly retarded by the inefficient culture techniques used for the propagation of these cells. The microcarrier culture system might be a good solution for this problem. DEAE-gels appear to be the most satisfactory microcarrier. The best results are obtained by using DEAE-gels with a rather low anionic exchange capacity or at higher capacity after coating with polymers.
D000837:Anion Exchange Resins; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D004171:Diploidy; D005782:Gels; D006801:Humans; D007475:Ion Exchange Resins; D014765:Viral Vaccines; D014776:Virus Cultivation / Q000379:methods*
D000837:Anion Exchange Resins; D003470:Culture Media; D005782:Gels; D007475:Ion Exchange Resins; D014765:Viral Vaccines