[Effect of insulin and hydrocortisone on the isoenzyme composition of pyruvate kinase in the rabbit kidney].
Alloxan diabetes, starvation of rabbits within one day and administration of hydrocortisone during 5 days did not cause distinct alterations in the total activity of pyruvate kinase in kidney cortex. At the same time pronounced alterations in the isozyme spectra were observed: the L-type activity of pyruvate kinase was decreased and the M2-type of activity was increased. The total activity and the isozyme content of pyruvate kinase were only slightly altered in starvation of rabbits during 3 or 10-16 days and after administration of protamine-Zn-insulin during 3 days. Hydrocortisone caused variable effects on the pyruvate kinase isozymes from kidney cortex, depending on periods of administration.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D005215:Fasting; D006854:Hydrocortisone / Q000494:pharmacology*; D049528:Insulin, Long-Acting / Q000494:pharmacology*; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D007672:Kidney Cortex / Q000032:analysis / Q000201:enzymology*; D008297:Male; D011770:Pyruvate Kinase / Q000032:analysis / Q000378:metabolism*; D011773:Pyruvates; D011817:Rabbits; D013997:Time Factors
D049528:Insulin, Long-Acting; D007527:Isoenzymes; D011773:Pyruvates; D011770:Pyruvate Kinase; D006854:Hydrocortisone
Vliianie insulina i gidrokortizona na izofermentnyĭ sostav piruvatkinazy v pochkakh krolika