Effect of aminonucleoside on the stability of rat liver ribosomal RNA to heating.
High molecular weight ribosomal RNA has been isolated from normal rat liver and from the livers of rats treated with aminonucleoside. Melting curves of the rRNA from treated rat livers were shifted to higher temperatures. The melting temperatures were found to be 62.5 and 66 degrees C for normal and treated material respectively. The rRNA was heated at different temperatures and analysed by polyacrylamide gel electrophoresis. In both normal and treated material, the 18 S was more stable in the treated material when compared with the control material.
D000818:Animals; D004355:Drug Stability; D006358:Hot Temperature; D008099:Liver / Q000032:analysis / Q000187:drug effects*; D008297:Male; D008970:Molecular Weight; D011691:Puromycin / Q000031:analogs & derivatives*; D011692:Puromycin Aminonucleoside / Q000494:pharmacology*; D012335:RNA, Ribosomal / Q000032:analysis; D051381:Rats
D012335:RNA, Ribosomal; D011691:Puromycin; D011692:Puromycin Aminonucleoside