Rosette formation of concanavalin A-treated erythrocytes around polymorphonuclear leucocytes and lymphocytes.
Concanavalin A (Con A) induces rosette formation of erythrocytes around polymorphonuclear leucocytes and lymphocytes in cell suspensions of autologous human blood cells. The effect which is most characteristic in a concentration between 25 and 50 microgram/ml is due to Con A bound on the erythrocyte membrane. A similar effect, although less pronounced, was observed with phytohaemagglutinin at concentrations of 10 and 25 microgram/ml. The treated erythrocytes showed a higher affinity to polymorphonuclears when compared with lymphocytes. At the contact area, the membrane of the erythrocyte became highly folded while its free surface was smooth and spherical. The effect of the local concentration and immunobilization of the lectin on the erythrocyte membrane and the similarity of the contact pattern to that of erythrophagocytosis are discussed.
D003208:Concanavalin A / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004903:Erythrocyte Aggregation; D004910:Erythrocyte Membrane / Q000276:immunology; D004912:Erythrocytes / Q000648:ultrastructure; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D012397:Rosette Formation
D003208:Concanavalin A