Cryoprecipitates and immune complexes: differentiation between cold insoluble immune complexes and nonspecific cryoprecipitates.
Cold insoluble circulating immune complexes occurred in 77% of rabbits immunized with 125I BSA. 9.8% of the total cryoprotein was 125I BSA. This represented 43% of the serum antigen. Tripling the antigen concentration in vitro decreased the amount of cold insoluble antigen by as much as 83%. In nonspecific cryoprecipitates only 0.4% of the total cryoprotein was 125I BSA. This represented only 0.028% of the serum antigen. Tripling of the antigen concentration in vitro increased the amount of cold insoluble antigen. Cold insoluble circulating immune complexes occur at a critical antigen-antibody ratio and can be differentiated from nonspecific cryoprecipitates.
D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens; D011232:Chemical Precipitation; D003080:Cold Temperature; D004306:Dose-Response Relationship, Immunologic; D007457:Iodine Radioisotopes; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology
D000936:Antigen-Antibody Complex; D000941:Antigens; D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine