[Glycine hydrazide as a bifunctional reagent for the synthesis of antigens with steroids as the immunodeterminant groups (author's transl)].
The suitability of glycine hydrazide as a link between steroids and carrier proteins in the synthesis of antigens was examined. Testosterone was used as hapten; bovine serum albumin as carrier protein. The reaction described here of testosterone with glycine hydrazide to form testosterone glycylhydrazone acetate took place under milk conditions and the yield was nearly quantitative. Rabbits immunized with the new antigen developed specific antibodies against testosterone.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis; D002417:Cattle; D005998:Glycine; D006834:Hydrazines; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine; D013739:Testosterone / Q000032:analysis / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D006834:Hydrazines; D012710:Serum Albumin, Bovine; D013739:Testosterone; D005998:Glycine
Glycinhydrazid als bifunktionelles Reagenz zur Synthese von Antigenen mit Steroiden als immundeterminante Gruppen