Effect of a sulfated polygalacturonic acid on the proliferation of cells in culture.
The effect of a low molecular weight sulfated polygalacturonic acid on the growth of BALB/c 3T3 cells and HeLa cells was examined. This chemical reduced the saturation density of 3T3 cells in the range of concentrations of 10(-4) to 1 mg/ml, and inhibited the proliferation of HeLa cells, causing little or no cell damage. Treated cells began to grow after removal of the sulfated polygalacturonic acid. Treatment with this acid also induced enlargement of the cell size in 3T3 cells, and a marked reduction of colony size and of central piling up in HeLa cells. 35S-sulfated polygalacturonic acid was bound to the cells immediately. Electrophoretic mobility of both cell lines treated with this sulfated polygalacturonic acid increased the negative charge of the cell surface. These evidences suggest that sulfated polygalacturonic acid affects the cell surface, restricting the cell growth of both cell lines.
D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis; D006367:HeLa Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000494:pharmacology*; D014574:Uronic Acids / Q000494:pharmacology*
D011134:Polysaccharides; D014574:Uronic Acids; D004247:DNA