[Modification of the micromethod of estimation of citric acid in the bone tissue].
A modified method is developed for estimation of citric acid in bone tissue. Citrate was isolated from acid bone hydrolysate without a preliminary deproteinization; then it was oxidized and converted into pentabromaceton by treatment of citric acid with bromine. The content of pentabromaceton was estimated by a photometric method. The method was highly sensitive and reproductible.
D000096:Acetone / Q000031:analogs & derivatives / Q000032:analysis; D000818:Animals; D001842:Bone and Bones / Q000032:analysis* / Q000378:metabolism; D002951:Citrates / Q000032:analysis* / Q000378:metabolism; D006868:Hydrolysis; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D013053:Spectrophotometry / Q000379:methods
D002951:Citrates; D000096:Acetone
Modofikatsiia mikrometoda opredeleniia soderzhaniia limonnoĭ kisloty v kostnoĭ tkani