The isolation of ovine lymphocytes and granulocytes from whole blood using hydroxyethylcellulose.
Sheep erythrocytes do not aggregate spontaneously but will sediment when mixed with a solution of hydroxyethylcellulose. The resulting leucocyte-rich supernatant can be used in the preparation of purified lymphocyte and granulocyte suspensions.
D000818:Animals; D001799:Blood Sedimentation; D002469:Cell Separation; D002482:Cellulose / Q000031:analogs & derivatives*; D006098:Granulocytes; D007962:Leukocytes; D008214:Lymphocytes; D008722:Methods; D012756:Sheep / Q000097:blood*
D002482:Cellulose