[Method of gradient chromatography for the isolation of purified lipid fractions from nerve tissue in studies using radioactive carbon, C14].
A method is developed for separation of lipids from nervous tissue by thin-layer chromatography in silica gel. Cholesterol and "minor" lipids were removed from the start by the first chromatography in the system ether-benzol-ethanol-acetic acid. Phospholipids and galactolipids, which remained at the start, were separated by the repeated chromatography in the system chloroform-methanol-NH4OH. The purified fractions of cholesterol, cerebrosides, sulfatides and the total fraction of phospholipids were obtained from 100-150 mg of nervous tissue in amounts, sufficient for estimation of their specific radioactivity in experiments with 14C.
D000818:Animals; D002250:Carbon Radioisotopes; D002554:Cerebrosides / Q000302:isolation & purification; D002784:Cholesterol / Q000302:isolation & purification; D002855:Chromatography, Thin Layer / Q000379:methods*; D005732:Gangliosides / Q000302:isolation & purification; D008055:Lipids / Q000302:isolation & purification*; D008297:Male; D009417:Nerve Tissue / Q000032:analysis*; D010743:Phospholipids / Q000302:isolation & purification; D011817:Rabbits; D013433:Sulfoglycosphingolipids / Q000302:isolation & purification
D002250:Carbon Radioisotopes; D002554:Cerebrosides; D005732:Gangliosides; D008055:Lipids; D010743:Phospholipids; D013433:Sulfoglycosphingolipids; D002784:Cholesterol
Metod stupenchatoĭ khromatografii dlia vydelenii ochishchennykh fraktsiĭ lipidov iz nervnoĭ tkani v issledovaniiakh s primeneniem radioaktivnogo ugleroda C14