Isolation, identification and quantitation of serum 5alpha-pregnane-3,20-dione and its relationship to progesterone in the pregnant mare.
5alpha-pregnane-3,20-dione was isolated from pooled pregnant mare serum using Sephadex LH-20 column chromatography and identified by the use of radioimmunoassay, gas-liquid chromatography and gas-liquid chromatography-mass spectrometry analyses. 5beta-pregnane-3,20-dione was not cross-reactive with the radioimmunoassay system and was not detected by gas-liquid chromatography. Peripheral blood levels of progesterone and 5alphs-pregnane-3,20-dione were determined by radioimmunoassay in four Quarter Horse mares for the first 150 days of gestation. Progesterone and 5alpha-pregnane-3,20-dione declined from a range of 6 to 15 ng/ml at day 10 to a range of 2 to 8 ng/ml at day 40. After day 40 there was an increase in progesterone and 5alpha-pregnane-3,20-dione concentration. Toward the end of 150 days of gestation progesterone tended to decline; whereas, 5alpha-pregnane-3,20-dione levels were maintained or increased to levels as high as 35 ng/ml. Neither source nor function of 5alpha-pregnane-3,20-dione is known.
D000818:Animals; D002849:Chromatography, Gas; D003429:Cross Reactions; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006736:Horses; D011247:Pregnancy; D011277:Pregnanediones / Q000097:blood*; D011374:Progesterone / Q000097:blood*; D011863:Radioimmunoassay; D013997:Time Factors
D011277:Pregnanediones; D011374:Progesterone