Mechanistic studies on transcorneal permeation of pilocarpine.
The mechanism of corneal pilocarpine penetration was studied in the albino rabbit using radiochemical techniques. The apparent rate and extent of pilocarpine accumulation in the aqueous humor and the various cell layers of the cornea were determined for both intact and abraded eyes. For the first time, drug levels were monitored in the epithelium and stroma-endothelium of the intact cornea using a tissue-scraping technique. In addition, a new postinstillation rinsing method was devised to evaluate the rate of corneal uptake. The results demonstrate a dual role for the corneal epithelium, both as a barrier to drug penetration and as a reservoir for drug in the intact cornea. The transcorneal pilocarpine flux is slower than the data appear to indicate, and previous overestimates of the apparent absorption rate constant are due to parallel elimination processes occurring at the absorption site. Pharmacokinetic parameters were determined for each tissue to generate an overall mechanism for corneal permeation.
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D003315:Cornea / Q000033:anatomy & histology / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D009883:Ophthalmic Solutions; D010862:Pilocarpine / Q000378:metabolism*; D011817:Rabbits; D013997:Time Factors
D009883:Ophthalmic Solutions; D010862:Pilocarpine