[Determination of the biosynthetic chemotherapeutic index of antiviral agents in cell culture].
An experimental substantiation of an improved approach to testing antiviral chemotherapeutic agents in tissue culture was done on the model of Venezuelan equine encephalomyelitis virus and primarily trypsinized chick embryo fibroblasts using some biologically active agents such as actinomycin D, RNA-ase, gentamycin, poly (rI). poly (rC). The approach is based on combined use of stationary cultivation of cells and virlses, a method for determination of radioactivity of the cells grown directly in vials for scintillation counting as well as methods for determination of virus-specific and cell syntheses by incorporation of labeled precursors. The features of the mechanism of action of the agents under study is discussed in terms of their influence on cell metabolism.
D000818:Animals; D000998:Antiviral Agents; D002642:Chick Embryo; D046508:Culture Techniques; D003609:Dactinomycin / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004666:Encephalitis Virus, Venezuelan Equine / Q000187:drug effects* / Q000378:metabolism; D005839:Gentamicins / Q000494:pharmacology; D011070:Poly I-C / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D012260:Ribonucleases / Q000494:pharmacology
D000998:Antiviral Agents; D005839:Gentamicins; D012367:RNA, Viral; D003609:Dactinomycin; D012313:RNA; D012260:Ribonucleases; D011070:Poly I-C
Opredelenie biosinteticheskogo khimioterapevticheskogo infeksa protivovirusnykh preparatov v kul'ture kletok