[Use of an ion exchanger as a rapid means of extraction, applied to the determination of urinary estrogens and pregnanediol in pregnancy, using colorimetry or gas chromatography].
The method described permits, after classical hydrolysis, extraction without a solvent of estrogens during pregnancy. It also permits estimation of pregnanediol which is eluted in a different fraction. Twenty estimations may be carried out in half a day on test samples of from 2 to 10 ml of urine. The estimation of total estrogen by colorimetry takes into consideration the efficiency of extraction by the use of a standard range treated under the same conditions. For gas chromatography, two internal standards are added to the urine after hydrolysis: these are 16,17-epiestriol and 5 beta pregnane-3 alpha, 17 alpha, 20 beta triol (PGTS) have respectively the same behaviour as estriol on the one hand and pregnanediol and pregnanetriol, on the other hand. They take into consideration resin extraction and chromatographic behaviour and may be used for quantitative estimation. The graphs of standardisation and recovery have been studied for the main estrogens together with pregnanediol. The various eluates were controlled by gas chromatography coupled to mass spectrometry.
D002849:Chromatography, Gas; D003124:Colorimetry; D004967:Estrogens / Q000302:isolation & purification / Q000652:urine*; D005260:Female; D006801:Humans; D007475:Ion Exchange Resins; D008722:Methods; D011247:Pregnancy; D011276:Pregnanediol / Q000302:isolation & purification / Q000652:urine*
D004967:Estrogens; D007475:Ion Exchange Resins; D011276:Pregnanediol
Utilisation d'un échangeur d'ions comme moyen rapide d'extraction appliqué au dosage des estrogènes et du prégnandiol urinaires de grossesse par colorimétrie ou par chromatographie en phase gazeuse