Predication of the effect of carcinostatic agents on tumor-bearing host by the sensitivity test using acid phosphatase activity in vitro.
For the purpose of predicting the effect of carcinostatic agents on tumor-bearing host in vitro, a sensitivity test of a drug in tumor bearers was developed. This test is based on the change in the specific activity of acid phosphatase of individual tumor and liver tissue, and the difference in them was used as an indicator of effectiveness. The value was called the "effective value." The present papar is concerned with the relationship between the effective value and effectiveness of the agents in rodents and man. The results showed that the effective value became higher with increasing concentration of Mitomycin-C incubated with tumor and liver tissues. The survival time of Ehrlich carcinoma-bearing mice, which were given Mitomycin-C intraperitoneally, was longer and increase in the dose of Mitomycin-C. In ascitic or subcutaneous form of Ehrlich carcinoma, AH-130, and Yoshida sarcoma, the effective value was parallel with the survival time of or the inhibition of tumor growth in tumor-bearing animals treated with Mitomycin-C. In 25 patients with gastric carcinoma treated with Mitomycin-C, the postoperative survival time was more closely correlated with the effective value calculated by the titers of both tumor and liver than the value of tumor alone.
D000135:Acid Phosphatase / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy / Q000201:enzymology; D004341:Drug Evaluation; D005260:Female; D006801:Humans; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008875:Middle Aged; D008937:Mitomycins / Q000494:pharmacology / Q000627:therapeutic use*; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000201:enzymology; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D012517:Sarcoma, Yoshida / Q000188:drug therapy / Q000201:enzymology; D013045:Species Specificity; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000201:enzymology
D008937:Mitomycins; D000135:Acid Phosphatase