The production and action of interferon in Chinese hamster cells.
The interferon system has been investigated in primary cell cultures established from Chinese hamster embryos and new born pups. Interferon synthesis was induced with Sindbis virus, ultraviolet inrradiated Newcastle disease virus (u.v.-NDV) and with polyriboinosine acid-polyribocytidylic acid complex [poly (rI). poly (rC)]. Only u.v.-NDV induced significant production of interferon, maximum amounts being produced in 'aged' cells. Its apparent mol. wt. was 25000. CHO-KI cells, an established line of Chinese hamster cells, did not synthesize interferon in response to viruses, but were sensitive to its action.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D046508:Culture Techniques; D004285:Dogs; D007372:Interferons / Q000096:biosynthesis* / Q000494:pharmacology; D008970:Molecular Weight; D009522:Newcastle disease virus / Q000528:radiation effects; D011070:Poly I-C; D012845:Sindbis Virus; D014466:Ultraviolet Rays
D007372:Interferons; D011070:Poly I-C