Biochemical studies of acetylcholine esterase inhibitor present in Eugenia caryophyllus.
Water, saline and ethanol extracts of Eugenia caryophyllus inhibited brain acetylcholinesterase (AchE) activity. Inhibitory action of the saline extract was greater than that of other extracts. The most active fraction of the saline extract obtained by chromatography through Sephadex G25 and DEAE cellulose columns and elution with tris and phosphate buffers (pH. 7.1) exhibited reactions characteristic of phenols, amino compounds and uronic acid. It is, therefore, suggested that the active inhibitor is a complex glycoside containing phenol and uronic acid part structure.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D055598:Chemical Phenomena; D002621:Chemistry; D002800:Cholinesterase Inhibitors; D002848:Chromatography, DEAE-Cellulose; D000431:Ethanol; D066298:In Vitro Techniques; D008297:Male; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000032:analysis*; D051381:Rats; D012965:Sodium Chloride; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014867:Water
D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D000431:Ethanol; D012965:Sodium Chloride