Assignment of a structural gene for beta-glucuronidase to human chromosome C7.
An electrophoretic technique was developed which allows the separation of human beta-glucuronidase (GUS EC 3.2.1.3.1) from the enzyme present in cultured murine. Chinese and Syrian hamster cells in one buffer system on Cellogel. Using this technique a number of independent human-mouse somatic cell hybrids have been analyzed for the segregation of GUS, other enzyme markers, and all human chromosomes. The results indicate that a structural gene for human beta-glucuronidase is located on chromosome C7.
D000818:Animals; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D004586:Electrophoresis / Q000379:methods; D005796:Genes; D005966:Glucuronidase / Q000096:biosynthesis*; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology / Q000648:ultrastructure; D066298:In Vitro Techniques; D051379:Mice; D010641:Phenotype
D005966:Glucuronidase