[Effect of 2,4-diamino-5-chlorphenyl-6-ethylpyrimidine on the oncorporation of P32-phosphate into adenylic nucleotides of rat embryo and placenta].
Teratogenic doses of antimalaria preparation, 2,4-diamino-5-p-chlorophenyl-6-ethylpyrimidine (chloridine), known in biochemistry as a specific inhibitor of dihydrofolate reductase, result in the decrease of 32P-phosphate incorporation into ATP and ADP, coupled with the inhibitions of DNA synthesis, in rat embryo and placenta on 13th day of development, while the incorporation of 14C-formiate into free nucleotides of acid soluble embryo and placenta fractions (at the same intervals after teratogen injections), is the same as in the control. These data show that the primary blocking of folate cycle and DNA biosynthesis in 13 days old embryos with induced anomalies of development is some way coupled with disturbances of ATP metabolism.
D000014:Abnormalities, Drug-Induced; D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D004247:DNA / Q000096:biosynthesis; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D005493:Folic Acid Antagonists; D005561:Formates / Q000037:antagonists & inhibitors; D010761:Phosphorus Radioisotopes; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011739:Pyrimethamine / Q000494:pharmacology*; D051381:Rats; D013723:Teratogens / Q000494:pharmacology*
D000962:Antimalarials; D002250:Carbon Radioisotopes; D005493:Folic Acid Antagonists; D005561:Formates; D010761:Phosphorus Radioisotopes; D013723:Teratogens; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D004247:DNA; D011739:Pyrimethamine
Vliianie 2,4-diamino-5-khlorfenil-6-etilpirimidina na vkliuchenie P32-fosfata v adenilovye nukleotidy embrionov i platsenty krys