title: Effects of progesterone and oestradiol on RNA and protein metabolism in the genital tract and on survival of embryos in the ovariectomized ewe.
abstract: The hormonal regulation of metabolism in the genital tract and the development of embryos during early pregnancy in the ewe have been examined. Ovariectomized ewes received injections of maintenance progesterone, oestrous oestradiol and priming progesterone according to schedules designed to simulate endogenous ovarian secretion during early pregnancy, around the time of oestrus and during the luteal phase of the oestrous cycle immediately preceding oestrus. The survival and development of embryos was dependent upon the dose of maintenaince progesterone and the duration of treatment at the time of transfer, but changes in progesterone dose did not change endometrial protein or RNA metabolism on particular days. Both priming progesterone and oestrous oestradiol were required for normal embryo development. Priming progesterone and oestrous oestradiol each increased endometrial RNA/DNA ratios during early pregnancy. There were no interactions between priming progesterone and oestrous oestradiol, their effects being simply additive. Neither maintenance nor priming progesterone had any effect on protein and RNA metabolism in the oviduct. It is suggested that in the intact ewe oestrogen secreted at oestrus and progesterone secreted prior to oestrus play important roles in the establishment of a uterine environment suitable for the subsequent normal development of embryos.
mesh_terms: D000818:Animals; D002369:Castration; D004622:Embryo, Mammalian / Q000502:physiology*; D004717:Endometrium / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005187:Fallopian Tubes / Q000378:metabolism; D005260:Female; D005836:Genitalia, Female / Q000378:metabolism*; D010053:Ovary / Q000502:physiology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects; D011374:Progesterone / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism*; D012756:Sheep / Q000502:physiology*
chemical_list: D011374:Progesterone; D004958:Estradiol; D012313:RNA