[Modification of the spectrophotometric method of determination of pyridoxal-5'-phosphate and pyridoxal in enzymatic preparations].
In quantitative measurements of pyridoxal-5'-phosphate and pyridoxal in enzymes routinely used phenylhydrasine was substituted for 4-nitrophenylhydrasine. This increased the sensitivity of the method by 70%. The modified procedure had another advantage: it allowed measurements of the optic density of resulting 4-nitrophenylhydrasones at 430 nm for acid solutions and at 550 nm for alkaline solutions.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D003593:Cytoplasm / Q000201:enzymology; D006834:Hydrazines; D007202:Indicators and Reagents; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000166:cytology; D006005:Phosphorylases / Q000032:analysis*; D011730:Pyridoxal / Q000032:analysis*; D011732:Pyridoxal Phosphate / Q000032:analysis*; D011817:Rabbits; D013053:Spectrophotometry / Q000379:methods*; D013552:Swine
D006834:Hydrazines; D007202:Indicators and Reagents; D011730:Pyridoxal; D011732:Pyridoxal Phosphate; D006005:Phosphorylases; D001219:Aspartate Aminotransferases
Modifikatsiia spektrofotometricheskogo metoda opredeleniia piridoksal'-5'-fosfata i piridoksalia v fermentnykh preparatakh