[Fractionation of DNA on unmodified cellulose].
Not only separation of native, two-stranded DNA from denatured molecules but more distinct fractionation of single-stranded DNA was demonstrated on unmodified powdery and microgranular cellulose. Denatured DNA, absorbed on cellulose, was eluted from a column as two asymmetric peaks by 0,12 M and 0.05 M NaCl (for I and II peaks, respectively). Absorption and fractionation of denatured DNA and poly-A containing RNA on unmodified cellulose was apparently achieved on the basis of the same principles.
D000818:Animals; D002482:Cellulose; D002845:Chromatography / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D003656:Decidua / Q000032:analysis; D005260:Female; D011247:Pregnancy; D051381:Rats
D002482:Cellulose; D004247:DNA
Fraktsionirovanie DNK na nemodifitsirovannoĭ tselliuloze