Evidence against the postulated identity of e-antigen with DNA polymerase associated with the hepatitis B candidate virus.
Partially purified preparations of e-antigen, obtained from sera of hepatitis B antigen carriers, were chromatographed on columns of immobilized single- or double-stranded DNA or on pyran-Sepharose. e-Antigen did not adsorb to any of these columns under conditions appropriate for the retention of various nucleic acid polymerases. Therefore, e-antigen and the DNA polymerase associated with Dane particles can be regarded as distinct proteins.
D002353:Carrier State / Q000097:blood; D004259:DNA-Directed DNA Polymerase / Q000276:immunology*; D006509:Hepatitis B / Q000097:blood; D006511:Hepatitis B Antigens / Q000032:analysis*; D006515:Hepatitis B virus / Q000201:enzymology / Q000276:immunology*; D006801:Humans
D006511:Hepatitis B Antigens; D004259:DNA-Directed DNA Polymerase