Cell proliferation and protein synthesis in human leukaemic myeloblasts after cytosine arabinoside therapy.
The effect of a single intravenous bolus injection of cytosine arabinoside (ARA-C) on the cytokinetics and 3H-leucine uptake of leukaemic myeloblasts in bone marrow and blood was studied at intervals up to 96 h after ARA-C in five patients with previously untreated acute myelogenous leukaemia. An early decrease in mitotic index and in 3H-thymidine labelling was observed in four of five patients and pretreatment values were reached again within the observation period. Changes suggesting synchronization were not observed. ARA-C induced a marked decrease in protein synthesis in the leukaemic myeloblasts as estimated from either a decrease in the 3H-leucine labelling index or the mean grain count of 3H-leucine labelled cells. It is suggested that ARA-C is incorporated also by cell which are not in S-phase and has a prolonged biochemical effect on the leukaemic cells.
D000328:Adult; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D003561:Cytarabine / Q000008:administration & dosage / Q000494:pharmacology*; D003864:Depression, Chemical; D005260:Female; D006801:Humans; D007275:Injections, Intravenous; D007948:Leukemia, Monocytic, Acute / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008938:Mitosis / Q000187:drug effects*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D013997:Time Factors
D009363:Neoplasm Proteins; D003561:Cytarabine