Effects of radiation and porphyrin on mitosis and chromosomes in human hematopoietic cell lines.
The effect on mitosis of a human hematopoietic cell line RPMI-1788 treated with a metal chelate (Zn++) of meso-tetra (p-carboxyphenyl) porphine (Zn-TCPP) alone at various concentrations or in combination with gamma-irradiation at various doses were studied. The results showed that both Zn-TCPP and radiation were effective in interfering with normal mitosis and that the effect of radiation was relatively more effective. Data also suggest interacting effects between Zn-TCPP and gamma-irradiation. At low doses of radiation, Zn-TCPP potentiated the effect of radiation. The reverse seemed to be true at a high dose of radiation. The effects of two porphyrins (Zn-TCPP and hematoporphyrin) and radiation on chromosomes were also studied. Chromosomal aberrations characteristic of radiation were observed. The porphyrins were found not to be effective chromosome-breaking agents under the experimental conditions tested.
D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects / Q000528:radiation effects*; D003037:Cobalt Radioisotopes; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000528:radiation effects*; D006801:Humans; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects*; D011166:Porphyrins / Q000494:pharmacology*; D011829:Radiation Dosage
D003037:Cobalt Radioisotopes; D011166:Porphyrins