Intermediates in the biosynthesis of double-stranded ribonucleic acids of bacteriophage phi 6.
Pseudomonas phaseolicola infected with bacteriophage phi 6 synthesized all three viral double-stranded RNA segments, three single-stranded RNAs, and three replicative intermediate-like RNAs in the presence of rifampin. The single-stranded RNA intermediates sedimented and electrophoresed along with melted viral double-stranded RNA, annealed with melted viral double-stranded RNA, and were transient in nature. The relative amounts of the single-stranded RNA intermediates varied during the infection cycle and were altered in the presence of chloramphenicol. The replicative intermediate-like RNAs sedimented faster than double-stranded RNA, failed to enter 2.5% polyacrylamide gels, eluted with double-stranded RNA from a CF-11 cellulose column, were precipitated with single-stranded RNA in 2 M LiC1, and yielded three genome-size pieces of double-stranded RNA upon digestion with RNase. These results are consistent with the hypothesis that complementary strands of the phi 6 double-stranded RNAs are synthesized asynchronously during the infection cycle.
D001435:Bacteriophages / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology; D003864:Depression, Chemical; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011549:Pseudomonas; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D012293:Rifampin / Q000494:pharmacology
D012367:RNA, Viral; D002701:Chloramphenicol; D012293:Rifampin