An evaluation of messenger RNA competition in the shutoff of human interferon production.
The process that shuts off poly(I)-poly(C)-induced interferon production in a strain of diploid human fibroblasts (FS-4)-involves the synthesis of new RNA, presumably nuclear heterogeneous RNA. When cultures in the shutoff phase of interferon production are treated with actinomycin D (5 mug/ml) or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB 40 muM), the rate of interferon production continues to decline for a further 3-4 hr, but then tends to level off. The treated cultures maintain interferon production at a reduced level for at least 10 hr. The residual rate of interferon production is higher in cultures which received actinomycin D or DRB early in the shutoff phase as compared to the rate in cultures treated late.
D001562:Benzimidazoles / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology; D007372:Interferons / Q000096:biosynthesis*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008954:Models, Biological; D011070:Poly I-C; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012263:Ribonucleosides / Q000494:pharmacology; D014529:Uridine / Q000378:metabolism
D001562:Benzimidazoles; D012333:RNA, Messenger; D012263:Ribonucleosides; D003609:Dactinomycin; D012313:RNA; D007372:Interferons; D007930:Leucine; D011070:Poly I-C; D014529:Uridine