Characterization of DNA condensates induced by poly(ethylene oxide) and polylysine.
High-molecular-weight DNA is known to collapse into very compact particles in a salt solution containing polymers like poly(ethylene oxide) [(EO)n] or polyacrylate. The biological relevance of this phenomenon is suggested by our recent finding that high concentrations of the highly acidic internal peptides found in the mature T4 bacteriophage head, as well as poly(glutamic acid) and poly(aspartic acid), can collapse DNA in a similar manner. The structure of DNAs collapsed by various methods has been studied with electron microscope. We find (EO)n collapses T4 or T7 bacteriophage DNA into compact particles only slightly larger than the size of the T4 and T7 head, respectively. In contrast, polylysine collapses DNA into different types of structures. Double-stranded DNA collapsed with (EO)n is cut by the single-strand specific Neurospora crassa endonuclease (EC 3.1.4.21) into small fragments. Extensive digestion only occurs above the critical concentration of polymer required for DNA collapse, demonstrating the (EO)n-collapsed DNA contains enzyme-vulnerable regions (probably at each fold), which are preferentially attacked. The size of the DNA fragments produced by limit-digestion with the nuclease ranges between 200 and 400 base pairs when DNA is collapsed by (EO)n. Only fragments of DNA which are larger than 600 base pairs are cut by the endonuclease in (EO)n-containing solution.
D004279:DNA, Viral / Q000032:analysis; D005027:Ethylene Oxide / Q000494:pharmacology; D008970:Molecular Weight; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D011095:Polyethylenes / Q000494:pharmacology*; D011107:Polylysine / Q000494:pharmacology*; D014779:Virus Replication
D004279:DNA, Viral; D010455:Peptides; D011095:Polyethylenes; D011107:Polylysine; D005027:Ethylene Oxide