title: The major cell surface glycoprotein of chick embryo fibroblasts is an agglutinin.
abstract: A major cell surface protein, CSP, of chick embryo fibroblasts has been shown to constitute up to 3% of total cell protein, and to be decreased after viral transformation. Its role in normal cell behavior is not known. We have isolated CSP from chick embryo fibroblasts by extraction with 1 M urea and find that these preparations of CSP agglutinate formalinized sheep erythrocytes at protein concentrations of under 2 mug/ml. In extracts of chick embryo cells, the quantity of such hemagglutinating activity parallels that of CSP determined by electrophoresis, and both are substantially decreased in chick cells transformed by the Bryan hightiter strain of Rous sarcoma virus. Both CSP and hemagglutinating activity are progressively adsorbed onto erythrocytes and can be released by 1 M urea. An antiserum to purified CSP specifically blocks the agglutination. The agglutinating activity is destroyed by boiling or treatment with proteases. The agglutination reaction is inhibited by the chelating agents EDTA and EGTA [ethyleneglycol-bis(beta-aminoethyl ether)N,N'-tetraacetic acid]. Agglutination is also inhibited to a lesser degres by amino sugars and other amines, increased osmolarity, and urea. Other monosaccharides, hyaluronidase, DNase, and RNase have little or not effect on the agglutination reaction. This demonstration that CSP has an agglutinating activity that is sensitive to proteases and that requires divalent cations suggests that this molecule may play a role in cell adhesion.
mesh_terms: D000373:Agglutinins / Q000032:analysis*; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002413:Cations, Divalent; D002462:Cell Membrane / Q000032:analysis*; D002642:Chick Embryo; D005347:Fibroblasts / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006386:Hemagglutination Tests; D012492:Salts; D013696:Temperature; D014508:Urea / Q000494:pharmacology
chemical_list: D000373:Agglutinins; D000596:Amino Acids; D002241:Carbohydrates; D002413:Cations, Divalent; D006023:Glycoproteins; D012492:Salts; D014508:Urea