Isolation and characterization of the protein coded by gene A of bacteriophage phiX174 DNA.
Replication of phiX174 circular replicative form (RFI) DNA by extracts of Escherichia coli infected with bacteriophage phiX174 (amber in gene A) requires the phiX174 gene A product. This requirement has been used as an assay for the isolation of this protein. The gene A product (purified 4000-fold) caused relaxation of superhelical phiX174 RFI and formation of discontinuities in the viral strand of phiX174 RFI uniquely situated in the A region of the genome, and yielded a complex after interacting with phiX174 RFI that is active in replication of phiX RFI.
D003090:Coliphages / Q000032:analysis* / Q000201:enzymology; D004261:DNA Replication; D004270:DNA, Circular / Q000096:biosynthesis; D004277:DNA, Single-Stranded / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D003852:Deoxyribonucleoproteins / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D005796:Genes; D009690:Nucleic Acid Conformation / Q000187:drug effects; D014764:Viral Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology
D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D003852:Deoxyribonucleoproteins; D014764:Viral Proteins; D004720:Endonucleases