title: Adenovirus-2 mRNA is transcribed as part of a high-molecular-weight precursor RNA.
abstract: The order of transcription and the length of nascent RNA transcripts from adenovirus-2 (Ad-2) DNA in the nucleus of infected cells has been deduced by labeling the growing RNA chains in vivo for a very brief period, separating the RNA on the basis of size, and hybridizine to the ordered EcoRI restriction endonuclease fragments derived from Ad-2 DNA. The majority of the virus-specific RNA molecules are synthesized as very high-molecular-weight units beginning at a common point at least 25-30,000 base pairs from one end of the Ad-2 DNA. These molecules can be reduced in size without further RNA synthesis. The experiments indicate the obligatory origin of Ad-2 mRNA from a high-molecular-weight precursor molecule.
mesh_terms: D000256:Adenoviridae / Q000378:metabolism*; D006367:HeLa Cells; D008954:Models, Biological; D008970:Molecular Weight; D009694:Nucleic Acid Precursors / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D009694:Nucleic Acid Precursors; D012333:RNA, Messenger; D012367:RNA, Viral