Studies of the mechanism of growth promotion of lymphoma cells by 2-mercaptoethanol in vitro.
Rates of incorporation of thymidine and uridine, but not leucine, decrease markedly in L1210 (V) cells within 1 hour of incubation in DULBECCO'S medium containing 10% serum. 2-Mercaptoethanol (2-ME) after a latent period of more than 5 hours causes an increase in nucleotide incorporation. Bovine serum albumin can substitute for serum in the medium, but a higher concentration of 2-ME is required for growth. After charcoal treatment of the albumin less 2-ME is required. These experiments suggest that inhibition of the tumor cells is caused by a serum factor whose effect is antagonized by the thiol.
D000818:Animals; D001345:Autoradiography; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D004220:Disulfides / Q000378:metabolism; D006133:Growth Substances; D007930:Leucine / Q000378:metabolism; D007939:Leukemia L1210 / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism*; D008623:Mercaptoethanol / Q000494:pharmacology*; D051379:Mice; D009374:Neoplasms, Experimental; D012710:Serum Albumin, Bovine; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D003470:Culture Media; D004220:Disulfides; D006133:Growth Substances; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D008623:Mercaptoethanol; D007930:Leucine; D013936:Thymidine; D014529:Uridine