Platelet-induced tolerance in the production of heteroantisera to human leukemia-associated antigens.
A method is described for raising antisera to human leukemia cells of an individual patient in mice rendered tolerant with cyclophosphamide to platelets obtained from the same patient. The resulting antisera are able to distinguish serologically between leukemic blast cells and remission cells of patients with acute leukemia and may be recognizing leukemia-associated antigens. The antisera are similar in activity to antisera raised following  tolerance-induction with remission leukocytes, but larger volumes of anti-leukemia antiserum can be raised using the more easily obtainable platelets. This technique provides further evidence that human platelets share many of the antigens present on human leukocytes.
D000208:Acute Disease; D000951:Antigens, Neoplasm / Q000008:administration & dosage*; D001792:Blood Platelets / Q000276:immunology*; D003520:Cyclophosphamide / Q000008:administration & dosage; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification*; D007108:Immune Tolerance; D007274:Injections, Intraperitoneal; D007938:Leukemia / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000276:immunology; D015470:Leukemia, Myeloid, Acute / Q000276:immunology; D012075:Remission, Spontaneous
D000951:Antigens, Neoplasm; D007106:Immune Sera; D003520:Cyclophosphamide