Structural and functional analysis of ribosome subunits isolated from mouse liver following a toxic dose of dimethylnitrosamine.
Ribosomal subunits, isolated from mouse liver after a single dose of 50 mg/Kg b. w. of DMN, have been tested for their structural and functional properties. CsCl analysis has shown that after DMN treatment no loss of structural proteins occurs in both 40 S and 60 S ribosome subunits. It has been also demonstrated that these subunits are able to synthetize in the poly-U programmed system at low Mg++ concentrations, in the presence of heterologous initiation factors, at the same efficiency as the corresponding controls.
D000818:Animals; D002500:Centrifugation, Isopycnic; D004128:Dimethylnitrosamine / Q000494:pharmacology / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D051379:Mice; D009602:Nitrosamines / Q000633:toxicity*; D010448:Peptide Initiation Factors / Q000494:pharmacology; D010649:Phenylalanine / Q000096:biosynthesis; D011072:Poly U / Q000494:pharmacology; D011132:Polyribosomes / Q000187:drug effects; D012335:RNA, Ribosomal / Q000032:analysis; D012270:Ribosomes / Q000187:drug effects* / Q000648:ultrastructure
D009602:Nitrosamines; D010448:Peptide Initiation Factors; D012335:RNA, Ribosomal; D011072:Poly U; D010649:Phenylalanine; D008274:Magnesium; D004128:Dimethylnitrosamine