Isolation of lymphocytes, granulocytes and macrophages.
This paper presents the standard procedure for isolating lymphocytes and granulocytes from blood, using the Isopaque-Ficoll technique. A procedure for isolating granulocytes and macrophages from peritoneal fluid is also described.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D005362:Ficoll; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice
D005362:Ficoll