Visualization of actin fibers associated with the cell membrane in amoebae of Dictyostelium discoideum.
Amoebae of Dictyostelium discoideum were attached to a surface coated with polylysine, and the upper portion of the cells was sheared off with a stream of buffer. Scanning and transmission electron microscopy showed that the cytoplasmic surface of the exposed membrane was covered with fibers consisting of actin-containing filaments. The actin was identified by its solubility properties and its ability to interact with muscle myosin.
D000199:Actins / Q000032:analysis*; D000656:Amoeba / Q000032:analysis* / Q000648:ultrastructure; D001665:Binding Sites; D002462:Cell Membrane / Q000032:analysis / Q000648:ultrastructure; D004023:Dictyostelium / Q000032:analysis* / Q000648:ultrastructure; D007709:Klebsiella; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009235:Myxomycetes / Q000032:analysis*; D011107:Polylysine; D011485:Protein Binding
D000199:Actins; D011107:Polylysine