Isolation of the bacteriophage lambda A-gene protein.
An in vitro assay for measuring the activity of the phage lambda A-gene product has been developed. The assay is based on the observation that A-donor extracts complement A minus extracts for packaging of exogenous immature lambda DNA into phage particles. A partial purification of the A-gene product activity using this assay is presented. A method is suggested by which this A protein-dependent in vitro system might be manipulated to analyze the mechanism of reforming the lambda cohesive termini during chromosome assimilation into phage precursors.
D002499:Centrifugation, Density Gradient; D002852:Chromatography, Ion Exchange; D003090:Coliphages / Q000032:analysis*; D004267:DNA Viruses / Q000032:analysis; D004279:DNA, Viral / Q000378:metabolism; D005796:Genes; D005838:Genotype; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification*
D004279:DNA, Viral; D014764:Viral Proteins