RNA synthesis in isolated brian nuclei after administration of d-lysergic acid diethylamide (LSD) in vivo.
RNA synthesis in isolated brain nuclei was analyzed 2.5 hr after the intravenous administration of d-lysergic acid diethylamide (LSD) to young rabbits. The drug stimulated transcription by 54% in brain stem nuclei and by 13% in cerebral hemisphere nuclei expressed over saline controls. Both nucleoplasmic and nucleolar RNA synthesis were increased. The main activity in the isolated nuclei assay was due to nucleoplasmic RNA polymerase, since alpha-amanitin reduced synthesis by over 70% in either drug or control treatments.
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D001933:Brain Stem / Q000378:metabolism / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D007700:Kinetics; D008238:Lysergic Acid Diethylamide / Q000494:pharmacology*; D008297:Male; D008345:Manganese / Q000494:pharmacology; D009183:Mycotoxins / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis*; D011817:Rabbits; D013268:Stimulation, Chemical; D014158:Transcription, Genetic
D009183:Mycotoxins; D009842:Oligopeptides; D008345:Manganese; D012313:RNA; D008238:Lysergic Acid Diethylamide