Effect of RNA synthesis on the binding of 3H-cortisol to nuclear ribonucleoprotein particles from rat liver carrying DNA-like RNA in vivo.
3H-cortisol was found to associate with rat liver nuclear 30S ribonucleoprotein particles carrying D-RNA in vivo. No interaction was detectable when RNA synthesis was inhibited by alpha-amanitine. The association appears to be specifically for RNP carrying RNA synthesized after the administration of cortisol to adrenalectomized rats. The DNA/protein/RNA ratio of rat liver nuclei was not effected by alpha-amanitine under our conditions. However, the drug caused a 5-10 fold decrease in nuclear uptake of cortisol. The results are discussed in relation to a supposed transfer of cortisol-receptor complexes from the chromatin template to the nascent RNA chains.
D000315:Adrenalectomy; D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA; D005260:Female; D006854:Hydrocortisone / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D009698:Nucleoproteins / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis* / Q000378:metabolism*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism*
D000546:Amanitins; D009698:Nucleoproteins; D011965:Receptors, Glucocorticoid; D012261:Ribonucleoproteins; D012313:RNA; D004247:DNA; D006854:Hydrocortisone