Measurement of human plasma motilin.
A specific radioimmunoassay has been developed for human plasma motilin. No cross reaction was seen with other gut hormones and only a single major peak of motilin immunoreactivity was found in plasma and gut extracts after gel chromatography. The motilin content of the human duodenum was 165.7+/-15.9 pmol/g wet weight and jejunum 37.5+/-2.8 pmol/g with negligible amounts in the rest of the gut. Fasting plasma motilin concentrations in 100 healthy subjects ranged from less than 5 to 300 pmol/l (mean 72 pmol/l).
D000328:Adult; D002850:Chromatography, Gel; D004386:Duodenum / Q000378:metabolism; D005215:Fasting; D005768:Gastrointestinal Hormones / Q000097:blood*; D006801:Humans; D007583:Jejunum / Q000378:metabolism; D008875:Middle Aged; D009037:Motilin / Q000097:blood* / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods*
D005768:Gastrointestinal Hormones; D009037:Motilin