Effect of estrogen on gene expression: purification of vitellogenin messenger RNA.
We report initial studies on estrogen-mediated regulation of egg yolk protein synthesis in the rooster. Egg yolk proteins are normally synthesized as a large precursor, vitellogenin, in the liver of the laying hen; roosters synthesize vitellogenin only when treated with estrogen. Polysomal RNA from the liver of estrogen-treated roosters was translated in a reticulocyte cell-free system, and the newly synthesized proteins were identified by a highly specific and sensitive indirect immunoprecipitation reaction. The messenger RNA that specifies vitellogenin has been purified more than 800-fold from rooster liver polysomal RNA by a combination of methods, including immunoprecipitation of polysomes and chromatography of RNA on poly(U)-Sepharose.
D000818:Animals; D002474:Cell-Free System; D002645:Chickens; D004958:Estradiol / Q000494:pharmacology*; D008074:Lipoproteins / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism; D008297:Male; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D013268:Stimulation, Chemical; D014158:Transcription, Genetic / Q000187:drug effects*; D014819:Vitellogenins / Q000096:biosynthesis*
D008074:Lipoproteins; D012333:RNA, Messenger; D014819:Vitellogenins; D004958:Estradiol