Cytochemical and cytogenetic findings in five human leukocyte long-term cultures (LAHL) of different origin.
Five human leukocyte long-term suspension cultures were investigated by means of cytochemical and cytogenetic methods. A striking resemblance in the morphology of these cells originating either from peripheral blood or from spleen of patients with or without hematological disorders was found. Lymphocytic origin of all five cultures is suspected. In the three cultures a diploid karyotype was found with some aberrations. In one culture, derived from the spleen of a patient with panmyelopathy the mosaic 45 XX C -/46 XX was detected. Anomalies of C group chromosomes were the most consistent type of chromosome aberrations in two lines. Both were EBV positive.
D000135:Acid Phosphatase / Q000378:metabolism; D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D000743:Anemia, Hemolytic; D001345:Autoradiography; D002460:Cell Line; D002869:Chromosome Aberrations; D004950:Esterases / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007951:Leukemia, Myeloid; D015470:Leukemia, Myeloid, Acute; D007962:Leukocytes / Q000201:enzymology / Q000378:metabolism; D008875:Middle Aged; D010544:Peroxidases / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D013154:Spleen; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D014316:Tritium; D012313:RNA; D007770:L-Lactate Dehydrogenase; D010544:Peroxidases; D004950:Esterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D013936:Thymidine