Transmissibility of sarcoid-specific granulomas in the footpads of mice.
Homogenate of 30 sarcoid lymph nodes was injected into the footpads of mice and 42% of the mice showed epitheloid-cell granulomas in the sites. However, the control lymph-node homogenates obtained from nonsarcoid patients also provoked similar granulomatous changes, although the positive rate was somewhat lower. In the granulomas a small amount of dust pigment remained, but no polarizing crystals were found in the sites or in the homogenate of either groups. No acid-fast bacilli, fungi, or other aerobic organisms were cultivated from the homogenate. Granuloma-formation activity remained after the homogenate had been sterilized in several ways and it existed in sediments of centrifugation of less than 2200 x g, but not in the supernate. The results seem to indicate that the granuloma formed in the footpads were the result of a local immunological reaction to the degenerated heterogenic protein of the injected and persistent homogenate.
D000818:Animals; D002498:Centrifugation; D004195:Disease Models, Animal; D004847:Epithelial Cells; D004848:Epithelium / Q000473:pathology; D005528:Foot; D006099:Granuloma / Q000635:transmission*; D006614:Hindlimb; D008198:Lymph Nodes / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D012507:Sarcoidosis / Q000209:etiology / Q000473:pathology / Q000635:transmission*; D013242:Sterilization