In vitro synthesis of large peptide molecules using glucosylated single-stranded bacteriophage T4D DNA template.
Denatured Bacteriophage T4D DNA is able to stimulate aminoacid incorporation into TCA-precipitable material in an in vitro protein synthesis system according to base DNA sequences. Newly synthesized polypeptides remain associated with ribosomes and have a molecular weight in range of 15,000 to 45,000 Daltons.
D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005960:Glucosides / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D009691:Nucleic Acid Denaturation; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D013698:Templates, Genetic
D004279:DNA, Viral; D005960:Glucosides; D006027:Glycosides; D008274:Magnesium