title: 7-Hydroxymethotrexate as a urinary metabolite in human subjects and rhesus monkeys receiving high dose methotrexate.
abstract: Human subjects and rhesus monkeys receiving the antitumor agent methotrexate at the high dose levels recently introduced into clinical use (greater than 50 mg/kg) excrete significant amounts of the metabolite 7-hydroxymethotrexate. The metabolite is not detected in these species after methotrexate therapy at conventional dose levels. The evidence indicates that in primates, the in vivo conversion of methotrexate to 7-hydroxymethotrexate is a dose-dependent phenomenon, with the enzyme system(s) catalyzing the reaction having a low affinity for the drug.
mesh_terms: D000328:Adult; D000818:Animals; D001859:Bone Neoplasms / Q000188:drug therapy; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy; D002648:Child; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy; D008253:Macaca mulatta; D008297:Male; D008727:Methotrexate / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000378:metabolism / Q000627:therapeutic use / Q000652:urine; D008875:Middle Aged; D012516:Osteosarcoma / Q000188:drug therapy; D013997:Time Factors
chemical_list: C011864:7-hydroxymethotrexate; D008727:Methotrexate