Isolation of mouse reticulocyte globin messenger ribonucleoprotein by affinity chromatography using oligo(T)-cellulose.
Mouse globin messenger ribonucleoprotein (mRNP) has been isolated from reticulocyte polysomes by affinity chromatography to oligo(T)-cellulose, using a procedure modified from that of Lindberg and Sundquist. The messenger RNA and protein moieties fo the mRNP are indistinguishable from those isolated by less rapid techniques, such as zonal ultracentrifugation.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D009698:Nucleoproteins / Q000235:genetics*; D011132:Polyribosomes / Q000032:analysis; D012333:RNA, Messenger / Q000302:isolation & purification*; D012156:Reticulocytes / Q000032:analysis; D012261:Ribonucleoproteins / Q000235:genetics* / Q000302:isolation & purification
D009698:Nucleoproteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D005914:Globins