Monitoring of murine osteosarcoma by serial alkaline phosphatase determinations.
To establish that alkaline phosphatase (AP) was released by osteosarcoma cells, we measured this enzyme in C3H/HeJ mice with im-implanted osteosarcoma and in in vitro cultures of neoplastic cells subjected to short-term incubation. We found that 10(5) osteosarcoma cells synthesized a significant amount of AP in vitro in 30 minutes at 37 degrees C. A good correlation existed between pulmonary metastatic tumors and the AP values. Serum AP measurements indicated approximate sizes of disseminated and localized tumors, but could not monitor early localized tumors.
D000469:Alkaline Phosphatase / Q000097:blood / Q000378:metabolism*; D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D008175:Lung Neoplasms / Q000201:enzymology; D051379:Mice; D008809:Mice, Inbred C3H; D009362:Neoplasm Metastasis; D009929:Organ Size; D012516:Osteosarcoma / Q000097:blood / Q000201:enzymology*; D012513:Sarcoma, Experimental / Q000097:blood / Q000201:enzymology; D013997:Time Factors
D000469:Alkaline Phosphatase