Synthesis of acetylcholine receptor during differentiation of cultured embryonic muscle cells.
Acetylcholine receptor, a component of the specialized muscle membrane, appears during the differentiation of embryonic myogenic cells in tissue culture. Demonstration of incorporation of the radioactive precursor L-[35S]methionine into purified receptor polypeptides is presented as evidence for its synthesis de novo. The identity of the purified radioactive species is established by cosedimentation of the [35S]receptor with [3H]alpha-toxin binding activity on sucrose gradients and by crossreaction with antiserum to purified acetylcholine receptor of Electrophorus electricus.
D000109:Acetylcholine / Q000378:metabolism*; D000205:Actomyosin / Q000096:biosynthesis; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002417:Cattle; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D009132:Muscles / Q000196:embryology / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D011950:Receptors, Cholinergic; D012910:Snake Venoms / Q000378:metabolism
D009124:Muscle Proteins; D011950:Receptors, Cholinergic; D012910:Snake Venoms; D000205:Actomyosin; D003402:Creatine Kinase; D000109:Acetylcholine