Radiation-induced murine leukemia ERLD in cell culture.
The lymphoblastic leukemia ERLD, induced by radiation in a C57BL/6 mouse, was established in culture. Three cell lines, ERLD/Y3, ERLD/T ERLD/Two, have been in culture for nearly three years. Their isolation and growth depended upon the presence of 2-mercaptoethanol, glutamine, and asparagine in the medium. The cell lines, except ERLD/T, possess the TL antigen, a characteristic of ERLD and of other murine leukemia cells in vivo and of normal thymus cells of certain mouse strains, but not of C57BL/6. A distinctive submetacentric marker chromosome is also common to ERLD and the derived cell lines. The successful establishment of ERLD in culture provides a malignant thymocyte-related cell system for studies in nutrition and immunobiology.
D000818:Animals; D000951:Antigens, Neoplasm; D001216:Asparagine; D002460:Cell Line; D002869:Chromosome Aberrations; D003470:Culture Media; D005973:Glutamine; D007942:Leukemia, Experimental / Q000235:genetics / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000276:immunology; D007953:Leukemia, Radiation-Induced / Q000235:genetics / Q000276:immunology; D008623:Mercaptoethanol; D051379:Mice; D008810:Mice, Inbred C57BL
D000951:Antigens, Neoplasm; D003470:Culture Media; D005973:Glutamine; D008623:Mercaptoethanol; D001216:Asparagine