Acid phosphatase of azurophilic granules of C3HST4 lymphoma.
A transplantable mouse lymphoblastic tumor with unusual azurophilic granules has been recently reported. The present tumor has been studied with Gomori and Novikoff methods for lysosomal marker, acid phosphatase. By electron microscopy enzyme was found in granules surrounded by single or double membrane, which displayed a wide morphologic range appearing as 1. vesicular, 2. multivesicular, 3. compound, 4. granular and 5. tubular bodies. The occurrence and the amount of enzyme activity in the granules appeared dependent on their morphology. It was least in vesicular bodies and most in granular organelles, characteristically at their periphery. Since the present tumor, labelled C3HST4, is an unusual rich source of azurophilic granules, it might be useful for further studies of lysosomal bodies. Their functions are poorly understood. Similar structures have been observed in normal human lymphocytes and those from chronic lymphocytic leukemia.
D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D003594:Cytoplasmic Granules / Q000201:enzymology* / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000201:enzymology; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology; D008223:Lymphoma / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D008566:Membranes / Q000648:ultrastructure; D051379:Mice; D008809:Mice, Inbred C3H; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental
D000135:Acid Phosphatase