title: Assembly of chick brain tubulin onto flagellar microtubules from Chlamydomonas and sea urchin sperm.
abstract: Flagellar microtubules from Chlamydomonas and sea urchin sperm were used as in vitro assembly sites for chick brain tubulin. Brain microtubules assembled onto the A-tubules, central tubules, and, to a limited extent, onto the distal ends of the axonemes at low tubulin concentrations and onto distal and proximal ends at high tubulin concentrations; however, the rate of assembly onto the distal end was always greater. The rate of neurotubule assembly onto axonemes was shown to be dependent upon tubulin concentration and a forward rate constant for assembly was determined.
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002645:Chickens; D002696:Chlamydomonas / Q000648:ultrastructure*; D005407:Flagella / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D008297:Male; D008870:Microtubules / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012617:Sea Urchins; D013094:Spermatozoa / Q000648:ultrastructure*; D014404:Tubulin / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D014404:Tubulin