Electrophoretic patterns of deadenylylated chorion and globin mRNAs.
Treatment of silkmoth chorion mRNAs with calf thymus RNase H (EC 3.1.4.34; RNA-DNA-hybrid ribonucleotidohydrolase) in the presence of oligo(dT) specifically and effectively removes the 3'-terminal poly(A) sequences. Excision of non-poly(A) fragments cannot be detected. Under these conditions, RNase H leads to increased electrophoretic homogeneity of rabbit globin mRNA, presumably as a result of removal of poly(A) sequences that are inherently variable in length. Treatment with RNase H converts the three diffuse zones of messages for the several chorion proteins into multiple sharp bands.
D000249:Adenosine Monophosphate / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D002823:Chorion / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005321:Extraembryonic Membranes / Q000032:analysis*; D005914:Globins / Q000096:biosynthesis; D009036:Moths / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis*; D012260:Ribonucleases
D012333:RNA, Messenger; D000249:Adenosine Monophosphate; D005914:Globins; D012260:Ribonucleases