Prevention of low temperature denaturation injury in T4Bo phage by low concentrations of traditional cryoprotective additives.
The well known cryoprotective additives glucose, sucrose, glycerol, dimethyl sulphoxide, polyvinylpyrrolidone, dextran and ammonium acetate have been found to prevent inactivation of T4Bo phage frozen in sodium bromide solutions. Their protective effects in this experimental system could not be accounted for by a colligative mechanism. It is proposed that they may act by modifying the structure of the unfrozen aqueous phase rather than by direct interaction with the phage.
D001965:Bromides; D003090:Coliphages / Q000187:drug effects; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003911:Dextrans / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005615:Freezing; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D009691:Nucleic Acid Denaturation; D009994:Osmolar Concentration; D011205:Povidone / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology
D001965:Bromides; D003451:Cryoprotective Agents; D003911:Dextrans; D000644:Quaternary Ammonium Compounds; D013395:Sucrose; D011205:Povidone; D005947:Glucose; D005990:Glycerol; D004121:Dimethyl Sulfoxide