Evidence against inhibition of the renin-angiotensinogen reaction by des-angiotensin substrate in the rat.
1. The possible inhibitory effect of des-angiotensin I substrate on the renin-substrate reaction was studied. For this purpose rat angiotensinogen was purified 50-fool from plasma of nephrectomized rats. Des-angiotensin substrate was prepared from the purified angiotensinogen preparation by reaction with immobilized hog renin (coupled to Sepharose). 2. In kinetic experiments it was found that desangiotensin I substrate in concentrations of 0-225 and 0-45 micronmol/l has no influence on the reaction between rat renin and rat angiotensinogen.
D000804:Angiotensin II / Q000031:analogs & derivatives* / Q000378:metabolism; D000808:Angiotensinogen / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D008297:Male; D051381:Rats; D000092502:Renin Inhibitors
D000804:Angiotensin II; D000808:Angiotensinogen; D000092502:Renin Inhibitors