Change in Ig expression of a human lymphoblastoid line during long-term culture.
A human lymphoblastoid line, in continuous culture for over 3 years, showed changes in Ig expression with time. The following pattern of events was suggested by studies of Ig synthesis and secretion performed at intervals. In a mass culture containing a heterogeneous cell population, Ig expression may shift because of selection acting on the different phenotypes. In human lymphoblastoid lines there may be a tendency for selection to favor IgMchi-producing cells. In clones, including those that synthesize multiple H chains, substantial determinism for Ig expression is present.
D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000378:metabolism; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D013997:Time Factors
D007136:Immunoglobulins