Presence of messenger specifying sequences in the DNA of chromatin subunits.
Messenger-specifying sequences in subunits of human lymphocyte chromatin were detected by hybridization of DNA complementary to cytoplasmic polyadenylylated RNA with DNA isolated from the subunits. Comparison of the kinetics and extents of hybridization of complementary DNA with chromatin subunit DNA and with nuclear DNA showed that most of the repetitive sequences and single copy sequences in mRNA are present in chromatin subunits. This result indicates that inclusion of a DNA sequence into the subunit does not prevent its transcription in vivo.
D002843:Chromatin / Q000648:ultrastructure*; D005732:Gangliosides / Q000032:analysis*; D005796:Genes; D006801:Humans; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*
D002843:Chromatin; D005732:Gangliosides; D012333:RNA, Messenger; D011061:Poly A