The in vitro addition of a polyadenylate sequence to the 3' end of phage Qbeta RNA and the biological activity of the product.
Terminal riboadenylate transferase, purified from calf thymus, has been used to add a poly(A) extension to the 3' end of Qbeta RNA. The modified Qbeta RNA retains full infectivity in a spheroplast assay system. However, the progeny viruses do not contain poly(A) termini, indicating an in vivo rectification of the in vitro alteration.
D000227:Adenine Nucleotides / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D003090:Coliphages / Q000378:metabolism* / Q000472:pathogenicity; D009711:Nucleotides / Q000032:analysis; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides / Q000378:metabolism*; D012328:RNA Viruses / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis / Q000378:metabolism*; D014316:Tritium
D000227:Adenine Nucleotides; D009711:Nucleotides; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D012367:RNA, Viral; D014316:Tritium