Ligand-induced redistribution of lymphocyte membrane ganglioside GM1.
Dynamic aspects of the binding of cholera toxin to lymphocyte membranes have been studied. We have shown that the receptor for this ligand--the GM1 ganglioside--can be laterally redistributed into aggregates and caps. Exogenous purified GM1 inserted into GM1-deficient human leukaemic cells can undergo a similar pattern of ligand-induced mobilisation. These observations may have important implications for both the general behaviour of cell surface glycolipids and the mode of action of cholera toxin on adenyl cyclase.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D005732:Gangliosides / Q000378:metabolism*; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000378:metabolism; D008024:Ligands; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D009000:Monocytes / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D011955:Receptors, Drug / Q000187:drug effects*; D014118:Toxins, Biological / Q000378:metabolism*; D014734:Vibrio cholerae
D005732:Gangliosides; D008024:Ligands; D011955:Receptors, Drug; D014118:Toxins, Biological; D009439:Neuraminidase