Secretion of a nerve growth factor by mouse neuroblastoma cells in culture.
Analyses of supernatant solutions from mouse C1300 neuroblastoma cultures by two independent immunoassays reveal that these cells secrete a factor which is immunochemically similar to mouse submaxillary gland nerve growth factor. The neuroblastoma factor is also biologically active in inducing neurite outgrowth from embryonic sensory ganglia-an effect that is completely blocked by specific antibody to nerve growth factor. Neuroblastoma cells are known to be functionally responsive to nerve growth factor, and the observation that they secrete a molecule like it may mean that these cells require or utilize the factor during growth in culture.
D000818:Animals; D001681:Biological Assay; D002478:Cells, Cultured; D002642:Chick Embryo; D003090:Coliphages / Q000276:immunology; D005724:Ganglia / Q000187:drug effects; D007118:Immunoassay; D009374:Neoplasms, Experimental / Q000378:metabolism; D009414:Nerve Growth Factors / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013363:Submandibular Gland / Q000032:analysis
D009414:Nerve Growth Factors