title: Snythesis of polypeptides with the properties of myosin light chains direct by RNA extracted from muscle cultures.
abstract: Polyadenylylated RNA, extracted from differentiating primary cultures of rat muscle and the myogenic cell line L8, directs the synthesis of polypeptides in the wheat germ cell-free system which comigrate with myosin light chains under several electrophoretic conditions. The peptides also associate specifically with heavy myosin subunits during dissociation-reassociation treatment. Intact cells of primary skeletal muscle cultrues and of the myogenic line synthesize predominantly two ligh chains. RNA extracted from primary muscle cultures directs the synthesis of a third polypeptide in the cell-free system, similar to the third light chain found in myosin extracted from adult rat thigh muscle. Products of cell-free systems directly by RNA extracted from fibroblasts, reticulocytes, and myeloma cells did not contain detectable amounts of similar polypeptides.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002474:Cell-Free System; D002478:Cells, Cultured; D008970:Molecular Weight; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D009218:Myosins / Q000096:biosynthesis*; D010452:Peptide Biosynthesis; D011061:Poly A / Q000302:isolation & purification; D011487:Protein Conformation; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D009218:Myosins