title: A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.
abstract: Ribosomal salt washes of Artemia salina embryos contain a protein(s) that binds [3H]m7GpppGpC and [3H]m7GpppGmpC, as measured by retention on nitrocellulose membrane filters. These oligonucleotides correspond in structure to the methylated 5'-terminal sequences (caps) present in many eukaryotic mRNAs. The cap binding protein does not bind the unmethylated counterparts of caps, e.g., [32P]GpppGpCp, or a derivative of m7GpppGmpC containing ring-opened m7G. None of the purified initiation factors IF-MP, IF-M2A, IF-M2B, IF-M3, or IF-MI binds the m7G-containing oligonucleotides.
mesh_terms: D001483:Base Sequence; D001667:Binding, Competitive; D033364:Decapoda; D006150:Guanine Nucleotides / Q000378:metabolism* / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008745:Methylation; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D010448:Peptide Initiation Factors; D011485:Protein Binding / Q000187:drug effects; D014176:Protein Biosynthesis / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D006150:Guanine Nucleotides; D010448:Peptide Initiation Factors; D011506:Proteins; D012333:RNA, Messenger; D008274:Magnesium