Radioimmunoassay for melatonin.
Rabbits immunized with human albumin conjugates of N-(p-chlorobenzoyl)-2-methyl-5-methoxyindole-3-acetic acid (indomethacin), 5-methoxytryptamine, and 2-methyl-5-methoxyindole-3-acetic acid produced antibodies that bound [3-H] melatonin. The serologic specificities for the binding of melatonin with antibodies from four antisera to these three immunogens were determined. Melatonin was measurable even at the 0.1 picomole level by a radioimmunoassay. Serotonin, N-acetylserotonin, 5-methoxytryptophol, 5-methoxytryptamine and 6-hydroxymelatonin can be ruled out as immunoreactive material with the use of the multiple antisera of different specificities.
D008735:5-Methoxytryptamine / Q000276:immunology; D000818:Animals; D000937:Antigen-Antibody Reactions; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000302:isolation & purification; D007211:Indoles / Q000276:immunology; D007213:Indomethacin / Q000276:immunology; D008550:Melatonin / Q000032:analysis* / Q000276:immunology; D008722:Methods; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012709:Serum Albumin
D007074:Immunoglobulin G; D007211:Indoles; D012709:Serum Albumin; D008735:5-Methoxytryptamine; D008550:Melatonin; D007213:Indomethacin