[Local reaction to the intradermal injection of PHA in healthy persons and in chronic lympholeukosis patients].
Phytohemagglutinin (PHA) that stimulates the lymphocytes to blast transformation in conditions of cellular cultures provokes local skin reaction, when applied intradermally. At the beginning that reaction is manifested with hypermia at the place of the application and later an infiltration is formed. In healthy subjects the infliltration reaches maximal dimensions on the 48th to the 72nd hour post PHA injection. The presence of lymphocytes was established with the cytologic examination of the material from that infilitration at the 24th hour, lymphoblasts and hyperbasophilic blast cells at the 48th and 72nd hour and single reactive lymphocytes were observed at the 96th hour. The infiltration appears later in patients with quiet development of chronic leukosis, only after the 96th hour and is with smaller demensions. Negligible infiltration is observed in malignant leukosis, its progress in time does not differ from that in healthy subjects. However, skin reaction in intraderma injection of PHA gives a certain orientation about the immune organism reactivity and in case of 1 chronic leaukosis--about the form and severity of the disease.
D000328:Adult; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007271:Injections, Intradermal; D037102:Lectins / Q000494:pharmacology*; D007945:Leukemia, Lymphoid / Q000276:immunology*; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D012882:Skin Tests; D013997:Time Factors
D037102:Lectins
Lokalna reaktsiia pri intradermalno imzhektirane na FKhA pri zdravi i pri bolni ot khronichna limfolevokoza