In vitro granulocytic stem cell clonogenic capacity of marrow and spleen cells from mice infected with Rauscher leukemia virus.
Bone marrow and spleen cells from early, midstage, and terminal Rauscher leukemia virus (RLV-A)-infected erythroleukemic mice were assessed for granulocyte stem cell (CFU-c) clongenic capacity in the semisolid agar culture assay. It was found that marrow CFU-c concentrations exceeded normal in early stages of this erythroid disease but returned to near normal values during mid- and terminal phases. Splenic CFU-c concentrations, on the other hand, were generally higher than control values for all stages of the disease. These results are discussed with reference to the pathogenesis of RLV-A disease.
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells; D006098:Granulocytes / Q000276:immunology*; D006400:Hematocrit; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology* / Q000473:pathology; D007962:Leukocytes / Q000276:immunology*; D051379:Mice; D009929:Organ Size; D011925:Rauscher Virus; D013154:Spleen / Q000276:immunology* / Q000473:pathology