[Properties of the cell-free protein synthesizing system from the fibroin region of the Bombyx mori silkgland].
Microsomes isolated from the fibroin region of the Bombyx mori silkgland synthesize in the cell-free system a glycin rich polypeptide or polypeptides presumably representing fibroin precursors. Besides microsomes the system requires ATP and ATP-generating system, GTP, soluble protein fraction and tRNA, glycine incorporation is inhibited by puromycin and cycloheximide. It is shown that the synthesis of a polypeptide with high Gly/Lys ratio requires soluble protein fraction isolated from the silk gland at the end of the instar V. When the soluble protein fraction from the larvoe at the early instar V is used the Gly/Lys ratio in the product is markedly lower. These results permit to suggest that fibroin synthesis may be regulated at the level of tRNA aminoacylation.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D002474:Cell-Free System; D003513:Cycloheximide / Q000494:pharmacology; D005349:Fibroins / Q000096:biosynthesis*; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D011691:Puromycin / Q000494:pharmacology; D012343:RNA, Transfer / Q000378:metabolism
D011691:Puromycin; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D005349:Fibroins; D012343:RNA, Transfer; D003513:Cycloheximide
Svoĭstva beskletochnoĭ sistemy belkovogo sinteza iz fibroinovogo otdela shelkootdelitel'noĭ zhelezy tutovogo shelkopriada Bomby x mori.