Characterization of two gangliosides from human leukemic polymorphonuclear leukocytes.
Eight bands of gangliosides, from human polymorphonuclear leukocytes were demonstrated by thin-layer chromatography. Bands 4 and 5 were isolated and purified in sufficient amounts to allow their biochemical identification by thin-layer chromatography, gas chromatography and sequential action of glycosidases and neuraminidase. The major ganglioside was characterised as N-acetylneuraminylgalactosyl-beta-N-acetylglucosaminyl-beta-galactosyl-beta-glucosylceramide. A second ganglioside was tentatively identified as N-acetylneuraminyl-galactosyl-beta-N-acetylglucosaminyl-beta-(N-acetylneuraminyl)galactosyl-beta-glucosylceramide. Both gangliosides isolated were hydrolysed by neuraminidase. However, treatment of the intact cells with neuraminidase did not alter the ganglioside pattern.
D002241:Carbohydrates / Q000032:analysis; D005732:Gangliosides / Q000097:blood / Q000302:isolation & purification; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood*; D009439:Neuraminidase; D012794:Sialic Acids / Q000032:analysis
D002241:Carbohydrates; D005732:Gangliosides; D012794:Sialic Acids; D009439:Neuraminidase