title: Hepatic phenylalanine 4-monooxygenase is a phosphoprotein.
abstract: Phenylalanine hydroxylase [phenylalanine 4-monooxygenase; EC 1.14.16.1; L-phenylalanine, tetrahydropteridine:oxygen oxidoreductase(4-hydroxylating)] isolated from rat liver is a phosphoprotein containing approximately 0.31 mumol of  protein-bound phosphate per mumol of subunit (50,000 molecular weight). When the enzyme is further phosphorylated in the presence of ATP and a 3'5'-cyclic-AMP-dependent protein kinase (EC 2.7.1.37; ATP:protein phsophotransferase), an additional 0.7 mumol of phosphate per mumol of subunit is introduced, bringing the total phosphate content up to about 1 mumol/mumol of subunit. This phosphorylation of the enzyme in vitro is accompanied by a 2.6-fold increase in hydroxylase activity when the activity is assayed in the presence of tetrahydrobiopterin. Partial proteolytic digestion of phenylalanine hydroxylase, which previously had been shown to activate the enzyme 20- to 50-fold [Fisher, D.B. & Kaufman, S. (1973) J. Biol. Chem. 248, 4345-4353], removes almost all of the phosphate from the enzyme.
mesh_terms: D000818:Animals; D001708:Biopterins / Q000031:analogs & derivatives / Q000378:metabolism; D003067:Coenzymes / Q000378:metabolism; D004789:Enzyme Activation; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010651:Phenylalanine Hydroxylase / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D003067:Coenzymes; D010750:Phosphoproteins; D001708:Biopterins; D010651:Phenylalanine Hydroxylase; D011494:Protein Kinases; C003402:sapropterin