Deletions of lambda phage locating a prm mutation within the rightward operator.
Two deletion-substitution mutations of phage lambda, spi-113 and spi-274, are shown to remove about half of the rightward operator OR. Physiological studies show that spi-113 is still repressible. Thus, the 50 or so nucleotides of OR deleted in this mutant are not absolutely essential for repression. prm-116, which inactivates the promoter essential for the maintenance of lambda repressor synthesis, is located within OR between the endpoints of spi-113 and spi-274.
D001483:Base Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D003090:Coliphages / Q000378:metabolism; D005809:Genes, Regulator; D009154:Mutation; D009876:Operon; D012333:RNA, Messenger / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D014764:Viral Proteins / Q000378:metabolism
D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins