Wholebody autoradiography: a beta-radioluminescence method for detecting tritium.
A method of increasing the sensitivity of X-ray film for the wholebody autoradiography of tritiated compounds is described. Thirty micrometre sections are sprayed with a scintillator, Diphenyl oxazole (PPO) and exposed to light sensitive (screen) X-ray film at -70 degrees C. The method permits a substantial reduction in the required dose of tritium and of the period of exposure of tissue sections.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D010080:Oxazoles; D051381:Rats; D014316:Tritium; D014965:X-Rays
D010080:Oxazoles; D014316:Tritium