The failure of granulocytes to produce transcobalamin I TC I.
The hypothesis that transcobalamin I (TC I) originates from granulocytes was tested by comparing the isoelectric focusing (IEF) patterns of the R-type binder (cobalophilin) of vitamin B12 of: 1) lysed granulocytes, 2) 24 h granulocyte output, and 3) plasma. The preparations came from the blood of 5 normal subjects and 4 with myeloproliferative states. The cobalophilin released into a culture of granulocytes resembled the binder in the granulocytes initially. TC I, defined as the alpha1 cobalophilin with components isoelectric between pH 2.9-3.35 and as a carrier of native plasma B12, was not released by granulocytes. The granulocyte binder of leukaemic granulocytes did not differ from the normal in content per cell or amount released.
D001798:Blood Proteins / Q000096:biosynthesis*; D002478:Cells, Cultured; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D007525:Isoelectric Focusing; D007938:Leukemia / Q000097:blood; D007951:Leukemia, Myeloid / Q000097:blood; D007962:Leukocytes / Q000378:metabolism*; D055728:Primary Myelofibrosis / Q000097:blood; D014155:Transcobalamins / Q000096:biosynthesis*; D014805:Vitamin B 12 / Q000097:blood
D001798:Blood Proteins; D014155:Transcobalamins; D014805:Vitamin B 12