title: Limits of accuracy obtainable in the direct determination by fluorimetry of fluorescent whitening agents on thin layer chromatograms.
abstract: The quantitative direct determination of fluorescent whitening agents (FWAs) by thin layer chromatography involves limits of detection of 0.001 to 0.0005 mug. Visual assessment is about ten to a hundred times less sensitive than photometric measurement on thin layer chromatograms. The standard deviations in 10 measurements of two substances with different degrees of light sensitivity for a single measurement with a limit of detection of the order of 0.005 mug were about +/-13% to 44%. In the higher concentrations normally used (0.5 mug), the standard deviations were only +/-5% for both substances.
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods*; D012995:Solubility; D013237:Stereoisomerism; D013267:Stilbenes / Q000032:analysis
chemical_list: D013267:Stilbenes