Stucture of the amino-acid accepting 3'-end of high-molecular-weight eggplant mosaic virus RNA.
The sequence of the first 59 nucleotides from the 3'-OH terminus of high-molecular-weight eggplant mosaic virus RNA has been determined by standard radio-chemical techniques. The fragment was identified among the products of partial T1 RNase digestion by making use of the reverse migration, at pH 2.5, of the 3'-OH terminal oligonucleotide. No abnormal bases were found. A model of secondary structure may be constructed for this fragment, which is known to fix valine in the presence of valyl-tRNA synthetase. Its relation to the structures of genuine tRNAs and to the 3'-OH termini of other viral RNAs that also accept amino acids is discussed.
D000596:Amino Acids / Q000378:metabolism; D009029:Mosaic Viruses / Q000032:analysis* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer; D013329:Structure-Activity Relationship
D000596:Amino Acids; D012343:RNA, Transfer