[Investigation of DNA complexes with histones F3 and F3+F2a2].
Characteristic viscosity, sedimentation constant and optical anisotropy were studied of the complexes formed between DNA and histone fractions F3 and F3+F2a2. The parameters mentioned continuously change with the increase of protein content within the complex. Analysis of experimental data shows that binding of a histone bads to a decrease of size and thermodynamic rigidity of the DNA molecule. On the basis of results obtained a model of F3 histone binding with DNA is suggested, amino acid sequence of this protein being taken into account. Comparison of behaviour of nucleohistones DNA+F3 and DNA+F1 studied previously testifies different way of binding of these histones to DNA.
D004247:DNA; D003852:Deoxyribonucleoproteins; D006657:Histones; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins; D011487:Protein Conformation; D013816:Thermodynamics; D014783:Viscosity
D003852:Deoxyribonucleoproteins; D006657:Histones; D009698:Nucleoproteins; D004247:DNA
Issledovanie kompleksov DNK s gistonami F3 i F3+F2A2.