In vitro transcription of chromatin in the presence of a mercurated nucleotide.
Mercurated uridine triphosphate has been used to label transcripts of chicken reticulocyte chromatin made with Escherichia coli RNA polymerase. The mercury-labeled RNA product can be completely separated from endogenous RNA sequences in the chromatin by passage through a sulfhydryl Sepharose column. Globin cDNA hybridization to the transcript shows that only 2.6 x 10-5 of the transcript is globin RNA. In contrast to this result, erythrocte chromatin transcript contains less than one tenth as many globin RNA sequences.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000378:metabolism*; D002846:Chromatography, Affinity; D004247:DNA; D004912:Erythrocytes / Q000378:metabolism*; D005914:Globins; D008297:Male; D008628:Mercury; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D012156:Reticulocytes / Q000378:metabolism*; D014158:Transcription, Genetic; D014500:Uracil Nucleotides
D002843:Chromatin; D012333:RNA, Messenger; D014500:Uracil Nucleotides; D005914:Globins; D004247:DNA; D008628:Mercury