Separation of human hemoglobins by DEAE-cellulose chromatography using glycine-KCN-NaC1 developers.
This chromatographic procedure uses DEAE-cellulose as ion exchanger and glycine-KCN-NaC1 solutions as developers. Blood samples from several adults and newborn infants with alpha, beta, delta, or gamma chains variants have been analysed. The hemoglobins are eluted as compact and symmetrical zones, and the separation of many hemoglobin types is greatly improved. The procedure is relatively fast, simple, and inexpensive.
D000328:Adult; D002848:Chromatography, DEAE-Cellulose; D003486:Cyanides / Q000494:pharmacology*; D014644:Genetic Variation; D005845:Georgia; D005998:Glycine / Q000494:pharmacology*; D006441:Hemoglobin A / Q000302:isolation & purification; D006448:Hemoglobin J / Q000302:isolation & purification; D006451:Hemoglobin, Sickle / Q000302:isolation & purification; D006454:Hemoglobins / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D010414:Pennsylvania; D012965:Sodium Chloride / Q000494:pharmacology*
D003486:Cyanides; D006451:Hemoglobin, Sickle; D006454:Hemoglobins; D012965:Sodium Chloride; D006448:Hemoglobin J; D006441:Hemoglobin A; D005998:Glycine