Synthesis of acetylcholine receptors by cultured chick myotubes and denervated mouse extensor digitorum longus muscles.
Mono-[125I]iodo- alpha - bungarotoxin - receptor complexes extracted from chick myotube cultures as well as from adult denervated extensor digitorum longus muscles of the mouse have been banded at their buoyant density in gradients of metrizamide-deuterium oxide. When cultures or denervated adult muscles are preincubated in media containing 2H- or 13C-substituted amino acids under conditions of active receptor accumulation, the mono-[125I]iodo-alpha-bungarotoxin-receptor complexes have an increased buoyant density and band in a position of higher density in the gradient relative to a marker of mono-[131I]iodo-alpha-bungarotoxin-receptor complexes extracted from cells preincubated in normal media. It is concluded that the accumulation of receptors on the surfaces of cultured chick myotubes and on the non-synaptic surfaces of extensor digitorum longus muscles following denervation are the result of de novo synthesis.
D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D051379:Mice; D009121:Muscle Denervation; D009124:Muscle Proteins / Q000032:analysis / Q000096:biosynthesis*; D009132:Muscles / Q000196:embryology / Q000294:innervation / Q000378:metabolism*; D011950:Receptors, Cholinergic
D002038:Bungarotoxins; D009124:Muscle Proteins; D011950:Receptors, Cholinergic