Transfer of glucose-1-phosphate from UDP-glucose to lipid acceptors in plants.
Particulated preparations from pea seedlings formed lipid-linked glucose from UDP (14C)-glucose and endogenous acceptors at 0 C. The product has been identified as a polyprenyl pyrophosphate-glucose based on the following results: a) the glucolipid is labile to mild acid hydrolysis; b) when chromatographed in alkaline solvents, glucose-1,2-cyclic phosphate is obtained; c) the pattern elution of a DEAE-cellulose column fits in well with a pyrophosphate derivative; d) exogenous polyprenyl phosphate stimulated the incorporation of glucose into lipids; e) when incubated with (beta-32P)-UDP-glucose, radioactivity is incorporated into lipids; f) the reaction is reversed by 5 mM UMP. It is suggested that the glucolipid is an intermediate in glycan biosynthesis.
D007887:Fabaceae; D005958:Glucosephosphates / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D010946:Plants, Medicinal; D011105:Polyisoprenyl Phosphate Sugars / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
D005958:Glucosephosphates; D006023:Glycoproteins; D011105:Polyisoprenyl Phosphate Sugars; D014539:Uridine Diphosphate Sugars; D014532:Uridine Diphosphate Glucose