Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease.
Single-stranded viral DNA of bacteriophage f1 is cleaved into specific fragments by endo R-HaeIII, a restriction endonuclease isolated from Hemophilus aegyptius. The sites of the single strand cleavage correspond to those of the double strand cleavage. A single-stranded DNA fragment containing only one HaeIII site is also cleaved by this enzyme. This observation suggests that the reaction of single-stranded DNA cleavage does not require the formation of a symmetrical double-stranded structure that would result from the intramolecular base-pairing between two different HaeIII sites. Other restriction endonucleases may also cleave single-stranded DNA.
D001483:Base Sequence; D002874:Chromosome Mapping; D003090:Coliphages; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004267:DNA Viruses; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D006190:Haemophilus / Q000201:enzymology*; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004720:Endonucleases; D004262:DNA Restriction Enzymes