Tolerance and dependence evoked by an endogenous opiate peptide.
Incubation of neuroblastoma X glioma hybrid cells for 12-97 hr with methionine-enkephalin results in an increase in adenylate cyclase activity [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] that is mediated by the opiate receptor. The results show that cells become tolerant to, and dependent upon, enkephalin.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D002478:Cells, Cultured; D004361:Drug Tolerance; D004789:Enzyme Activation; D005048:Etorphine / Q000378:metabolism; D005910:Glioma; D006801:Humans; D006822:Hybrid Cells; D008024:Ligands; D009270:Naloxone / Q000494:pharmacology; D009447:Neuroblastoma; D009842:Oligopeptides / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology; D019966:Substance-Related Disorders / Q000201:enzymology*
D008024:Ligands; D009842:Oligopeptides; D011458:Prostaglandins E; D009270:Naloxone; D005048:Etorphine; D000262:Adenylyl Cyclases