Effect of metabolic inhibitors on bone lipid synthesis from radioactive bases.
Iodoacetate at 10(-3) M and 10(-4) M concentrations was found to strongly inhibit incorporation of both 14C-choline and 3H-inositol into phospholipids of calvaria from newborn rats; it had somewhat less effect on ethanolamine incorporation and almost no effect on serine incorporation. Similar though less dramatic results were observed with cyanide or dinitrophenol at similar concentrations.
D000818:Animals; D000831:Animals, Newborn; D000963:Antimetabolites / Q000494:pharmacology*; D001842:Bone and Bones / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002794:Choline / Q000378:metabolism; D003486:Cyanides / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D004983:Ethanolamines / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D007461:Iodoacetates / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D010718:Phosphatidylserines / Q000096:biosynthesis; D051381:Rats; D012694:Serine / Q000378:metabolism; D014316:Tritium
D000963:Antimetabolites; D002250:Carbon Radioisotopes; D003486:Cyanides; D004140:Dinitrophenols; D004983:Ethanolamines; D007461:Iodoacetates; D008055:Lipids; D010718:Phosphatidylserines; D014316:Tritium; D012694:Serine; D007294:Inositol; D002794:Choline