Relationship between nuclear and cytoplasmic poly(adenylic acid).
The kinetics of accumulation of radioactive poly(A) in the nucleus and cytoplasm of mouse L cells have been determined using labeling conditions in which the cellular ATP pool is shown to have a nearly constant specific radioactivity. Most or all nuclear poly(A) accumulates with kinetics very similar to those of heterogeneous nuclear RNA, having a half-time of about 25 min. There is little or no lag before attainment of a maximal rate of accumulation of cytoplasmic poly(A). These data are consistent with a variety of models in which nuclear poly(A) does or does not all serve as a precursor of cytoplasmic poly(A). In either type of model there must be a class of poly(A) either synthesized in the cytoplasm or passing through a small nuclear pool separate from the main pool of nuclear poly(A).
D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D007700:Kinetics; D007739:L Cells; D008954:Models, Biological; D011061:Poly A / Q000302:isolation & purification / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA