Increased ion permeability of planar lipid bilayer membranes after treatment with the C5b-9 cytolytic attack mechanism of complement.
The ion permeability of planar lipid bilayers, as measured electrically, was found to increase modestly upon treatment with purified complement complex C5b,6 and complement components C7 and C8. The subsequent addition C9 greatly amplified this change. No permeability changes occurred when components were added individually to the membrane, or when they were used in paired combinations, or when C5b, C7, C8, and C9 were admixed prior to addition. Thus, there is a significant parallel between the permeability changes induced in the model membrane and damage produced in biological membranes by the C5b-9 complement attack sequence. The efficiency of membrane action by C5b-9 was critically dependent on the order in whcih components were added to the membrane. There were also differences in the electrical properties of membranes treated with C5b-8 and C5b-9, though in both cases the enhanced bilayer permeability is best attributed to the formation of trans-membrane channels. Collectively, the data are consistent with the hypothesis that the mechanism of membrane action by complement involves the production of a stable channel across the lipid bilayer, resulting in cell death by colloid-osmotic lysis.
D002463:Cell Membrane Permeability / Q000187:drug effects*; D003182:Complement C5 / Q000494:pharmacology; D003183:Complement C6 / Q000494:pharmacology; D003184:Complement C7 / Q000494:pharmacology; D003185:Complement C8 / Q000494:pharmacology; D003186:Complement C9 / Q000494:pharmacology; D003165:Complement System Proteins / Q000494:pharmacology*; D004553:Electric Conductivity; D007477:Ions; D007700:Kinetics; D008567:Membranes, Artificial
D003182:Complement C5; D003183:Complement C6; D003184:Complement C7; D003185:Complement C8; D003186:Complement C9; D007477:Ions; D008567:Membranes, Artificial; D003165:Complement System Proteins