Molecular vehicle properties of the broad host range plasmid RK2.
The pladmid RK2 is stably maintained in a broad range of gram-negative bacteria. The RK2 DNA has a single Eco RI restriction site. The insertion of a DNA fragment into this site does not interfere with either plasmid maintenance or self-transmissibility. Because RK2 has a broad host range, it should be useful for the construction in vitro of hybrid plasmid molecules capable of being established by conjugal transfer or transformation into many genera of gram-negative organisms.
D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004352:Drug Resistance, Microbial; D005111:Extrachromosomal Inheritance; D005818:Genetic Engineering; D008970:Molecular Weight; D010957:Plasmids; D011815:R Factors; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes