title: Characterization of a gamma-carboxyglutamic acid-containing protein from bone.
abstract: A gamma-carboxyglutamic acid-containing protein has been purified from the calcified tissues of several vertebrates. The presence of three-gamma-carboxyglutamic acid residues in the bovine protein was established by alkaline hydrolysis and amino acid analysis, a method based upon studies with synthetic gamma-carboxyglutamic acid. The identity of gamma-carboxyglutamic acid in the bovine protein was established by mass spectroscopy on the unknown amino acid isolated from alkaline hydrolysates.
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002417:Cattle; D003804:Dentin / Q000032:analysis; D003864:Depression, Chemical; D005260:Female; D005269:Femur / Q000032:analysis*; D005399:Fishes; D006801:Humans; D006882:Hydroxyapatites / Q000378:metabolism; D008074:Lipoproteins / Q000096:biosynthesis*; D008297:Male; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D014233:Tricarboxylic Acids; D014819:Vitellogenins / Q000096:biosynthesis* / Q000097:blood
chemical_list: D006882:Hydroxyapatites; D008074:Lipoproteins; D011506:Proteins; D014233:Tricarboxylic Acids; D014819:Vitellogenins