Attempts to transform murine hemopoietic cells by Rauscher leukemia virus.
Primary Rauscher leukemia virus (RLV)-induced myeloid leukemias can produce many small clones in agar in the absence of a factor needed for the proliferation of normal myeloid cells. It seems that leukemic cells can more efficiently utilize the small amount of colony-stimulating factor (CSF) that is produced by them. At optimal stimulation by exogenous CSF, leukemic cells exhibit a poorer rate of proliferation than normal bone marrow cells. Hemopoietic cells can replicate the virus after infection in vitro. In some experiments, infection of normal bone marrow cells leads to the production of some cells with the same growth pattern as cells from primary leukemias.
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D006412:Hematopoietic Stem Cells; D007942:Leukemia, Experimental / Q000209:etiology; D007951:Leukemia, Myeloid / Q000209:etiology; D051379:Mice; D008807:Mice, Inbred BALB C; D011925:Rauscher Virus; D014779:Virus Replication