title: Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.
abstract: Reovirus mRNA synthesized in vitro by the virus-associated RNA polymerase in the presence of S-adenosylmethionine contains blocked, methylated 5'-termini with the structure, m-7G(5')ppp(5')G-MpCp. The functional significance and possible mechanism of formation of this novel 5'-5' terminal nucleotide linkage are discussed.
mesh_terms: D000818:Animals; D001483:Base Sequence; D001894:Borohydrides / Q000378:metabolism; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004792:Enzyme Precursors / Q000378:metabolism; D005838:Genotype; D006160:Guanosine Triphosphate / Q000378:metabolism; D008745:Methylation; D051379:Mice; D010761:Phosphorus Radioisotopes; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D012087:Reoviridae / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism; D014158:Transcription, Genetic; D014316:Tritium; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D001894:Borohydrides; D002250:Carbon Radioisotopes; D004792:Enzyme Precursors; D010761:Phosphorus Radioisotopes; D011498:Protein Precursors; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D012436:S-Adenosylmethionine; D006160:Guanosine Triphosphate; D012321:DNA-Directed RNA Polymerases