[Influence of UV-light on erythrocyte membrane structure and catalytic behaviour of membrane acetylcholine esterase].
UV-light is shown to induce the structural transitions in the erythrocyte membrane described by S-shape curves in plots of the structural response versus the irradiation dose. In contrast to the free acetylcholine esterase (AChE) UV-light acts on the membrane enzyme as a mixed inhibitor (simultaneous change in Vmax and Km). The modification of the environment structure of residual enzyme is suggested to be the main reason of this phenomenon. The effect is under the control of membrane integrity and disappears after its desintegration. Membrane AChE treated ultrasonically both prior to and after irradiation is inactivated without a Km change. The data obtained show the influence of erythrocyte membrane structure on the catalytic behaviour of membrane-bound AChE.
D000110:Acetylcholinesterase / Q000097:blood* / Q000528:radiation effects; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000528:radiation effects* / Q000648:ultrastructure; D004912:Erythrocytes / Q000528:radiation effects*; D006801:Humans; D007700:Kinetics; D014466:Ultraviolet Rays
D000110:Acetylcholinesterase
Vliianie UF-sveta na strukturu membran zritrotsitov i na kataliticheskie svoĭstva membrannoĭ atsetilkholinzsterazy.