Structure of a soluble super-active insulin is revealed by the nature of the complex between cyanogen-bromide-activated sepharose and amines.
Insulin-like material with elevated insulin specific acitivity is released from insulin-Sepharose in the presence of bovine-serum albumin. The mechanism of release and the chemical nature of this insulin-like material are revealed by the finding that amine-Sepharose is O-Sepharose-N-substituted isourea. Nucleophilic attack by amino groups releases N-1-N-2-disubstituted guanidines. Correspondingly, it is shown that the super-active insulin-like material is an N-1-N-2-disubstituted guanidine in which insulin and bovine-serum albumin are the substituents.
D000588:Amines; D000818:Animals; D003488:Cyanogen Bromide; D005947:Glucose / Q000378:metabolism; D006146:Guanidines; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D008820:Mice, Obese; D011134:Polysaccharides; D012685:Sepharose; D012710:Serum Albumin, Bovine; D013329:Structure-Activity Relationship
D000588:Amines; D006146:Guanidines; D007328:Insulin; D011134:Polysaccharides; D012710:Serum Albumin, Bovine; D012685:Sepharose; D005947:Glucose; D003488:Cyanogen Bromide