Methylation of messenger RNA of Newcastle disease virus in vitro by a virion-associated enzyme.
Purified Newcastle disease virus contains an enzyme that incorporates the methyl group from S-adenosyl-L-methionine into RNA synthesized in vitro by the virion-associated RNA polymerase (RNA nucleotidyltransferase). Incorporation of radioactivity from S-adenosyl-L-[methyl-3H]methionine was totally dependent upon RNA synthesis. The methylation reaction was completely inhibited by S-adenosyl-L-homocysteine, suggesting the transfer of only the methyl group of S-adenosyl-methionine to RNA products. Velocity sedimentation and hybridization of the in vitro product RNA indicated that both [3H]methyl and [32P]GMP labels resided in single-stranded 18S RNA molecules which were virus specific. Approximately 1 to 2 methyl groups were incorporated per RNA molecule. DEAE-cellulose chromatography of product RNA after alkaline hydrolysis suggested that the 5' terminus was the site of methylation.
D002848:Chromatography, DEAE-Cellulose; D004229:Dithiothreitol / Q000494:pharmacology; D006150:Guanine Nucleotides / Q000378:metabolism; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D008780:Methyltransferases / Q000378:metabolism*; D009522:Newcastle disease virus / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D012435:S-Adenosylhomocysteine / Q000494:pharmacology; D012436:S-Adenosylmethionine / Q000378:metabolism; D010948:Viral Plaque Assay
D006150:Guanine Nucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D008345:Manganese; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; D008780:Methyltransferases; D004229:Dithiothreitol