Cell-free synthesis of human interferon.
With mRNA prepared from induced human fibroblasts biologically active human interferon was synthesized de novo in a cell-free extract from mouse cells. The identity of the antiviral activity as human interferon was demonstrated by its species and antigenic specificity.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002645:Chickens; D003513:Cycloheximide / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007372:Interferons / Q000096:biosynthesis*; D051379:Mice; D011070:Poly I-C / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D013033:Sparsomycin / Q000494:pharmacology; D013045:Species Specificity
D012333:RNA, Messenger; D013033:Sparsomycin; D007372:Interferons; D003513:Cycloheximide; D011070:Poly I-C