The viral DNA replication machinery of adenovirus 12.
The viral DNA replication complex was found in the M band after fractionation. However, it did not associate with the nuclear membrane, but was found in association with types II and IV inclusions. It is suggested, therefore, that the viral DNA replication complex is the inclusions types II and IV. The analysis with Ad12 ts mutants showed the involvement of three viral genes in initiation of viral DNA replication, in formation of the viral DNA replication complex and the inclusions types II and IV. Temperature sensitivity of the gene products involved in viral DNA replication was also examined. From the data presented, the following sequence of viral DNA replication in Ad12-infected cells is suggested: (1) Synthesis of a protein (gene A product), which may be an acceptor or may modify the nuclear constituent to be an acceptor for parental viral DNA, to form the viral DNA replication complex; (2) synthesis of a protein (gene B product), which may be required for the parental viral DNA to associate with the acceptor and to form a type II inclusion-like structure (precursor of type II inclusion?); (3) synthesis of a protein (gene C product), which may be required for activation of the complex or type II inclusion and to alter type II inclusion to type IV inclusion. Further studies are necessary to substantiate the suggestion.
D000256:Adenoviridae / Q000378:metabolism*; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D003513:Cycloheximide / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis; D003864:Depression, Chemical; D009154:Mutation; D013696:Temperature; D013997:Time Factors; D014764:Viral Proteins / Q000378:metabolism
D000956:Antigens, Viral; D004279:DNA, Viral; D014764:Viral Proteins; D003513:Cycloheximide