Changes in ribosomal RNA processing paths in resting and phytohemagglutinin-stimulated guinea pig lymphocytes.
Processing of rRNA was examined in resting and phytohemagglutinin-stimulated guinea pig lymphocytes. Synthesis of 1.7 (28S) and 0.7 (18S) X 10(6) dalton rRNA was more than 4-fold greater in phytohemagglutinin-stimulated than in resting cells. A 5- to 10-fold increase in flux of molecules through a 2.3 X 10(6) dalton RNA occurred without a concurrent change in the flux through a 2.6 X 10(6) dalton fraction in phytohemagglutinin-stimulated cells. In both resting and phytohemagglutinin-stimulated lymphocytes, the 2.3 X 10(6) dalton intermediate equilibrated with [3H]methyl label and pulse-chased prior to the 2.6 X 10(6) dalton RNA. The data indicate at least two processing paths in guinea pig lymphocytes; one proceeds to rRNA via a 2.3 X 10(6) dalton intermediate, and another proceeds via a 2.6 X 10(6) dalton RNA. The increase in rRNA synthesis in phytohemagglutinin-stimulated cells occurs primarily through that path containing the 2.3 X 10(6) dalton intermediate.
D000818:Animals; D006168:Guinea Pigs; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D008970:Molecular Weight; D009694:Nucleic Acid Precursors / Q000096:biosynthesis / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000096:biosynthesis / Q000378:metabolism*; D013268:Stimulation, Chemical; D014158:Transcription, Genetic / Q000187:drug effects
D037102:Lectins; D009694:Nucleic Acid Precursors; D012335:RNA, Ribosomal