Recombinant DNA molecules of bacteriophage phi chi174.
Phi chi174 DNA structures containing two different parental genomes were detected genetically and examined by electron microscopy. These structures consisted of two monomeric double-stranded DNA molecules linked in a figure 8 configuration. Such DNA structures were observed to be formed preferentially in host recA+ cells or recA+ cell-free systems. Since the host recA+ allele is required for most phi chi174 recombinant formation, we conclude that the observed figure 8 molecules are intermediates in, or end products of, 1 phi chi174 recombination event. We propose that recombinant figure 8 DNA molecules arise as a result of "single-strand aggression," are stabilized by double-strand "branch migration," and represent a specific example of a common intermediate in genetic recombination.
D002474:Cell-Free System; D003090:Coliphages / Q000378:metabolism* / Q000648:ultrastructure; D004267:DNA Viruses / Q000378:metabolism / Q000648:ultrastructure; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D008854:Microscopy, Electron; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic
D004277:DNA, Single-Stranded; D004279:DNA, Viral