A modified procedure for the preparation of di- and triribonucleotides from pancreatic ribonuclease digest of RNA.
A novel procedure for the separation of oligonucleotides from pancreatic RNase-digest of RNA is described. The method involves a group-separation of uracil-containing and of cytosine-containing nucleotides on Dowex 50W. The obtained groups are further separated on DEAE-Sephadex A-25 by a linear gradient of NH4HCO3.
D002852:Chromatography, Ion Exchange; D003597:Cytosine Nucleotides / Q000302:isolation & purification; D008722:Methods; D009841:Oligonucleotides / Q000302:isolation & purification*; D009843:Oligoribonucleotides / Q000302:isolation & purification*; D010179:Pancreas / Q000201:enzymology; D012313:RNA / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D014500:Uracil Nucleotides / Q000302:isolation & purification
D003597:Cytosine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D014500:Uracil Nucleotides; D012313:RNA; D012260:Ribonucleases