[Role of salivary lipids in the precipitation of a salivary glycoprotein under the effect of calcium].
During its purification, a calcium precipitable glycoprotein, isolated from human mixed saliva was submitted to dialysis. When purified, this glycoprotein did not precipitate with the addition of calcium. The study of the precipitation conditions showed that during dialysis salivary lipids passed in the dialysate. Dialysated saliva gave no precipitate by calcium addition. Precipitation experiments of dialysated saliva and reprecipitation of the purified glycoprotein by calcium, in presence or absence of salivary lipids, showed that these latter were indispensable for the precipitation of the calcium precipitable salivary protein. These results demonstrated a possible role played by salivary lipids in the elaboration of dental plaque, whose matrix contained relatively important lipid quantities.
D000596:Amino Acids / Q000032:analysis; D002118:Calcium / Q000494:pharmacology*; D002241:Carbohydrates / Q000032:analysis; D011232:Chemical Precipitation; D003956:Dialysis; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000032:analysis / Q000494:pharmacology*; D012463:Saliva / Q000032:analysis / Q000378:metabolism
D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D008055:Lipids; D002118:Calcium
Rôle des lipides salivaires dans la précipitation d'une glycoprotéine salivaire sous l'effet du calcium