Heterogeneity of carcinoembryonic antigen. II. Monosaccharide composition of concanavalin A-reactive and -nonreactive CEA.
Monosaccharide compositions of Con A-reactive CEA (CEA-M) and Con A-nonreactive CEA (CEA-P) separated from two different samples of CEA were analysed by gas liquid chromatography. It was revealed that all CEA subfractions possessed N-acetylglucosamine, fucose, and galactose residues. One out of 4 subfractions did not contain sialic acid and another one lacked glucose in its carbohydrate moiety. N-acetylgalactosamine was not detected in measurable amount in any of the 4 subfractions. A large amount of mannose was found in CEA-M, but only a small amount in CEA-P.
D000116:Acetylgalactosamine / Q000032:analysis; D000117:Acetylglucosamine / Q000032:analysis; D001665:Binding Sites; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002846:Chromatography, Affinity; D003208:Concanavalin A; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology; D008358:Mannose / Q000032:analysis; D009362:Neoplasm Metastasis; D010190:Pancreatic Neoplasms / Q000276:immunology; D011485:Protein Binding; D012004:Rectal Neoplasms / Q000276:immunology; D012794:Sialic Acids / Q000032:analysis
D002272:Carcinoembryonic Antigen; D012794:Sialic Acids; D003208:Concanavalin A; D005643:Fucose; D000116:Acetylgalactosamine; D005944:Glucosamine; D008358:Mannose; D000117:Acetylglucosamine; D005690:Galactose