Synthesis of RNA containing a methylated blocked 5' terminus by HeLa nuclear homogenates.
RNA synthesized in vitro by HeLa nuclei contains a variety of blocked methylated 5'-terminal structures that can be grouped into four general types: (a) m(7)GppN(m)p...; (b) m(7)GppN(m)pM(m)p...; (c) m(7)GpppN(m)p...; and (d) m(7)GpppN(m)pM(m).... Both N(m) and M(m) can be either one of the four 2'-O-methylated nucleosides. The relative amounts of the N(m) species are G = 45%, A = 25%, U = 20%, and C = 10%.
D002467:Cell Nucleus / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000032:analysis; D006367:HeLa Cells / Q000378:metabolism*; D008745:Methylation; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D012260:Ribonucleases; D012436:S-Adenosylmethionine / Q000378:metabolism
D006150:Guanine Nucleotides; D012334:RNA, Neoplasm; D012436:S-Adenosylmethionine; D012260:Ribonucleases