Purification and characterization of the retina-specific cell-aggregating factor.
The tissue-specific, cell-aggregating component of embryonic neural retina cells was purified from the retina cell-aggregating factor and characterized as a glycoprotein. Its molecular weight in solution is in the range of 50,000, and it contains 10-15% carbohydrate. The amino-acid and carbohydrate compositions have been determined. The glycoprotein is produced by embryonic neural retina cells in primary monolayered cultures and is released into the medium. Its tissue-specific, cell-aggregating effect requires integrity of the polypeptide portion, but not of the carbohydrate portion. We suggest that the isolated molecule is a specific determinant of the embryonic retina cell-surface and that it is involved in mediating self-recognition and selective adhesiveness of these cells.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002449:Cell Aggregation / Q000187:drug effects*; D002642:Chick Embryo; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D008970:Molecular Weight; D012160:Retina / Q000032:analysis* / Q000166:cytology / Q000196:embryology
D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins