[A simple photometric test for the routine determination of delta-aminolaevulinic acid in urine for the control of lead exposure].
A simple and accurate method for determining urinary concentration of delta-aminolaevulinic acid (delta-ALA) is described. It is based on an improved application of the principle, first introduced by Tomokuni and Ogata, that a colourless pyrrole derivative is produced by the action of acetic acid ethyl ester on delta-ALA, which develops a violet colour on addition of Ehrlich's reagent. The influence of interfering substances was found to account for 20 plus or minus 3% in all examined urines samples. It is, therefore, recommended that an aqueous delta-ALA standard be prepared which contains only 80% of the concentration used in the calculation. Exact values are obtained in this way and the need for setting up a calibration curve by adding delta-ALA is avoided.
D000622:Aminolevulinic Acid / Q000652:urine*; D002021:Buffers; D005221:Fatigue / Q000139:chemically induced; D001068:Feeding and Eating Disorders / Q000139:chemically induced; D006261:Headache / Q000139:chemically induced; D006801:Humans; D007202:Indicators and Reagents; D007855:Lead Poisoning / Q000175:diagnosis / Q000652:urine*; D007982:Levulinic Acids / Q000652:urine*; D008722:Methods; D010783:Photometry; D013272:Stomach Diseases / Q000139:chemically induced; D014717:Vertigo / Q000139:chemically induced
D002021:Buffers; D007202:Indicators and Reagents; D007982:Levulinic Acids; D000622:Aminolevulinic Acid
Eine einfache, routinemssig durchführbare photometrische Bestimmung der Delta-Aminolävulinsäure im Harn zur Uberwachung bleigefährdeter Personen