Preparation and therapeutic potential of rabbit antisera with "directed" specificities for mouse leukemias.
Several immunization regimens for preparation of ALK-NABS were compared. One series of eight intravenous injections spaced over 5 weeks gave an ALK-NABS with potency and specificity that could be bettered only slightly by a second series of four injections spaced over 2 weeks, whereas a third series of injections was deleterious. Use of late immune antisplenocyte NABS for such immunizations produced ALK-NABS reagents with the highest in vitro specificity to leukemia cells relative to splenocytes after absorption, whereas early immune antisplenocyte NABS gave ALK-NABS with the highest antileukemia specificity relative to thymocytes. Therapy experiments with leukemias L1210 and BW-A showed increased survival times for isogeneic mice injected intraperitoneally with 10(3) (L1210 only), 10(4), and 10(5) (higher significance for L1210) cells, when ALK-NABS was given intraperitoneally in high dose on 4 or 5 successive days starting 1 day after inoculation of leukemia cells. In additional experiments with 10(5) cells given intraperitoneally, lower doses of ALK-NABS were progressively more effective with L1210 leukemia, producing some survivors without any apparent toxicity from the antiserum. In contrast, a similar experiment with leukemia BW-A was entirely negative. Addition of guinea pig serum to already excessive amounts of antiserum was not helpful.
D000818:Animals; D000951:Antigens, Neoplasm; D004351:Drug Resistance; D005260:Female; D006384:Hemagglutination; D007106:Immune Sera; D007114:Immunization; D007116:Immunization, Passive; D007939:Leukemia L1210 / Q000188:drug therapy / Q000276:immunology / Q000628:therapy; D007942:Leukemia, Experimental / Q000276:immunology / Q000628:therapy*; D008297:Male; D008727:Methotrexate / Q000627:therapeutic use; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D011817:Rabbits / Q000276:immunology
D000951:Antigens, Neoplasm; D007106:Immune Sera; D008727:Methotrexate