Canine osteosarcoma karyotypes from an original tumor, its metastasis, and tumor cells in tissue culture.
Radiation-induced osteosarcoma, its metastasis, and cells grown in tissue culture were karyotyped. Both hypodiploid and hyperdiploid stem lines were observed. The hypodiploid line contained 45-55 chromosomes with 10-15 abnormal metacentric and submetacentric chromosomes and one subtelocentric marker. The hyperdiploid line contained 90-105 chromosomes with 20-30 abnormal metacentric and submetacentric chromosomes with two subtelocentric markers. Karyotypic analysis can be used to monitor osteosarcomas maintained in tissue culture.
D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D066298:In Vitro Techniques; D007621:Karyotyping; D008175:Lung Neoplasms / Q000276:immunology; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000276:immunology; D012516:Osteosarcoma / Q000276:immunology*