title: Specific binding of the first enzyme for histidine biosynthesis to the DNA of histidine operon.
abstract: Studies were done to examine direct binding of the first enzyme of the histidine biosynthetic pathway (phosphoribosyltransferase) to 32P-labeled phi80dhis DNA and competition of this binding by unlabeled homologous DNA and by various preparations of unlabeled heterologous DNA, including that from a defective phi80 bacteriophage carrying the histidine operon with a deletion of part of its operator region. Our findings show that phosphoribosyltransferase binds specifically to site in or near the regulatory region of the histidine operon. The stability of the complex formed by interaction of the enzyme with the DNA was markedly decreased by the substrates of the enzyme and was slightly increased by the allosteric inhibitor, histidine. These findings are consistent with previous data that indicate that phosphoribosyltransferase plays a role in regulating expression of the histidine operon.
mesh_terms: D001276:ATP Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D006639:Histidine / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D009876:Operon; D010430:Pentosyltransferases / Q000235:genetics*; D010754:Phosphoribosyl Pyrophosphate / Q000494:pharmacology; D011485:Protein Binding; D012483:Salmonella Phages / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D006639:Histidine; D010754:Phosphoribosyl Pyrophosphate; D000255:Adenosine Triphosphate; D010430:Pentosyltransferases; D001276:ATP Phosphoribosyltransferase