[Integration and transfection of an arbovirus by mammalian cells].
A system: L cells chronically infected with Sindbis virus was studied. Unlike acute infection wherein the mature virions are produced, the chronically infected tissue culture produces subviral structures-infectious ribonucleoproteins. Molecular hybridization experiments revealed the integration of the viral genome (DNA-transcript) into the cellular genome. Transfection experiments showed the possibility to induce the synthesis of the virus in sensitive cells treated with DNA from the chronically infected cells.
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D007739:L Cells / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D012845:Sindbis Virus / Q000502:physiology*; D014162:Transfection; D014779:Virus Replication
D004279:DNA, Viral; D004247:DNA
Integratsiia i transfektsiia arbovirusa kletkami mlekopitaiushchikh.