Murine leukemia virus: detection of unintegrated double-stranded DNA forms of the provirus.
Infection of JLS V-9 (mouse bone marrow) cells with Moloney murine leukemia virus leads to appearance of virus-specific DNA in the low molecular weight DNA fraction from infected cells. Most of this DNA is double-stranded. Two discrete forms of double-stranded DNA, both of molecular weight 6 times 10-6, have been characterized. One of these forms is closed-circular, supercoiled DNA; the other form is a mixture of nicked-circular and linear DNA.
D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004270:DNA, Circular / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D051379:Mice; D008970:Molecular Weight; D008979:Moloney murine leukemia virus / Q000032:analysis*; D009690:Nucleic Acid Conformation
D004270:DNA, Circular; D004279:DNA, Viral