Glucose transport in Streptococcus mutans: preparation of cytoplasmic membranes and characteristics of phosphotransferase activity.
Cytoplasmic membranes from Streptococcus mutans were used to study phosphoenolpyruvate (PEP)-dependent glucose transport. The Km values for glucose and PEP were 0.3 and 0.4 mM. The system was sensitive to sulfhydryl reagents and detergents. The nonmetabolizable analogue 2-D-deoxyglucose was readily phosphorylated while alpha-methyl-glucose and 3-0-methylglucose were inefficiently derivatized. The fermentable sugars fructose, galactose, mannitol, and sorbitol were phosphorylated at reduced rates.
D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D003847:Deoxyglucose / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005958:Glucosephosphates / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D005988:Glyceric Acids / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D013012:Sorbitol / Q000378:metabolism; D013291:Streptococcus / Q000201:enzymology* / Q000378:metabolism
D005958:Glucosephosphates; D005988:Glyceric Acids; D009943:Organophosphorus Compounds; D005632:Fructose; D008353:Mannitol; D013012:Sorbitol; D010728:Phosphoenolpyruvate; D003847:Deoxyglucose; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D010770:Phosphotransferases; D005947:Glucose; D005690:Galactose