Preliminary studies on affinity labeling of the tubulin-colchicine binding site.
The present results argue that irradiation of a colchicine-tubulin complex at 366 nm results in destruction of the binding site and the labeling of a small molecule soluble in chloroform-methanol.
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism* / Q000528:radiation effects; D002642:Chick Embryo; D002725:Chloroform; D002850:Chromatography, Gel; D003078:Colchicine / Q000031:analogs & derivatives / Q000378:metabolism*; D003956:Dialysis; D006146:Guanidines; D000432:Methanol; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011485:Protein Binding; D011489:Protein Denaturation; D011830:Radiation Effects; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D014404:Tubulin / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D006146:Guanidines; D009419:Nerve Tissue Proteins; D014404:Tubulin; D012967:Sodium Dodecyl Sulfate; D002725:Chloroform; D003078:Colchicine; D000432:Methanol