Effect of N6-(delta2-isopentenyl)adenosine treatment in vivo on hexokinase activity of mouse L 1210 cells.
The kinetic properties of hexokinase of L1210 ascites tumor cells propagated in DBA/2HaD mice are altered by treatment of the mice with the modified nucleoside N6-(delta2-isopentenyl)-adensone (IPA). Relative to animals not treated with IPA, ascites cell hexokinase showed an increased affinity for ATP and a decreased affinity for glucose as a result of IPA treatment. The heat stability of the enzyme was different in treated and untreated mice. It was concluded that IPA treatment may either produce changes in enzyme conformation which resulted in a change in control mechanisms or may induce the formation of a hexokinase isoenzyme.
D000241:Adenosine / Q000031:analogs & derivatives*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism*; D006358:Hot Temperature; D007527:Isoenzymes / Q000378:metabolism; D007541:Isopentenyladenosine / Q000494:pharmacology*; D007700:Kinetics; D007939:Leukemia L1210 / Q000201:enzymology*; D051379:Mice; D008811:Mice, Inbred DBA; D011487:Protein Conformation; D011489:Protein Denaturation
D007527:Isoenzymes; D007541:Isopentenyladenosine; D000255:Adenosine Triphosphate; D006593:Hexokinase; D005947:Glucose; D000241:Adenosine