Phylogenetic origins of immune recognition: lymphocyte surface immunoglobulins in the goldfish, Carassius auratus.
Membrane immunoglobulin (Ig) of splenocytes and thymocytes of the goldfish, Carassius auratus, was demonstrated by indirect fluorescent-antibody techniques. Observations on shedding and resynthesis indicated that the thymocyte Ig was endogenously produced. The lymphocyte surface proteins were radioiodinated using the lactoperoxidase-catalyzed reaction, and the labeled Ig molecules were isolated by specific precipitation and analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The IgM-like membrane Igs of splenocytes and thymocytes were shown to differ in their ease of solubilization with nonionic detergent, and in the sodium dodecyl sulfate/electrophoretic mobility of their heavy chains. The significance of these observations for the evolution of T-cell recognition is discussed.
D000818:Animals; D005075:Biological Evolution; D003513:Cycloheximide / Q000494:pharmacology; D003530:Cyprinidae / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006054:Goldfish / Q000276:immunology*; D007075:Immunoglobulin M / Q000032:analysis / Q000378:metabolism; D007136:Immunoglobulins / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000096:biosynthesis / Q000378:metabolism*; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D007075:Immunoglobulin M; D007136:Immunoglobulins; D011947:Receptors, Antigen, B-Cell; D003513:Cycloheximide