title: O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.
abstract: O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products. Picomole quantities of amino acids, peptides, and proteins can be detected easily. o-Phthalaldehyde is five to ten times more sensitive than fluorescamine and is soluble and stable in aqueous buffers.
mesh_terms: D000447:Aldehydes; D000588:Amines / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D001572:Benzofurans; D005453:Fluorescence; D005914:Globins / Q000032:analysis; D008623:Mercaptoethanol; D009555:Ninhydrin; D010455:Peptides / Q000032:analysis; D010795:Phthalic Acids / Q000031:analogs & derivatives; D013141:Spiro Compounds
chemical_list: D000447:Aldehydes; D000588:Amines; D000596:Amino Acids; D001572:Benzofurans; D010455:Peptides; D010795:Phthalic Acids; D013141:Spiro Compounds; D008623:Mercaptoethanol; D005914:Globins; D009555:Ninhydrin