A beta-D-galactoside binding protein from electric organ tissue of Electrophorus electricus.
Extracts of electric organ tissue of Electrophorus electricus contain a saccharide-binding protein, named electrolectin, which agglutinates trypsin-treated rabbit erythrocytes and is specifically inhibited by disaccharides containing nonreducing terminal beta-D-galactosyl residues. Electrolectin seems at least partially membrane-bound but is also found in soluble fractions of homoge-nates from which it can be purfied by affinity chromatography on cross-linked and desulfated agarose (ECD-Sepharose) as a protein of molecular weight 33,000. About 400 mg of electrolectin are present per kg of tissue. It has an affinity for lactose of 1.0 mM-1 and 5.5mM-1 as estimated, respectively, by hapten inhibition and fluorescence spectroscopy. Studies on the distribution of beta-D-galactoside-binding activity in animal tissues reveal particularly high levels in sheletal muscle tissue and in cultures of embryonic skeletal muscle and neuroblastoma cells.
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002642:Chick Embryo; D002846:Chromatography, Affinity; D004557:Electric Organ / Q000032:analysis / Q000378:metabolism*; D004593:Electrophorus; D004912:Erythrocytes / Q000276:immunology; D005697:Galactosides / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D006386:Hemagglutination Tests; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009928:Organ Specificity; D011485:Protein Binding; D011817:Rabbits; D011955:Receptors, Drug; D013050:Spectrometry, Fluorescence; D013347:Subcellular Fractions / Q000032:analysis / Q000378:metabolism; D013862:Thiogalactosides / Q000378:metabolism
D005697:Galactosides; D006027:Glycosides; D009419:Nerve Tissue Proteins; D011955:Receptors, Drug; D013862:Thiogalactosides