Vitamin B12-binding proteins of r-type, cobalophilin.
A gradient was developed for isoelectric focusing in the pH range 2-5. Cobalophilin (earlier called R proteins or vitamin B12-binding proteins of R-type) was isolated from saliva and amniotic fluid in homogeneous form. It was found to be a glycoprotein with a molecular weight of 59,300-69,100. The preparation from amniotic fluid contained 33% carbohydrate. Cobalophilin variants in plasma, serum, granulocytes, platelets, amniotic fluid, milk, saliva and gastric juice were characterized by isoelectric focusing. Most fluids and cells contained the same isoproteins, with pI values between 2.3 and 5.0. Isoproteins of presumably myelogenic origin (e.g. those in granulocytes and plasma) had pI values below 4.2, whereas those of glandular origin (in milk and saliva) had a pI range of 4.0-5.0. Serum contained more cobalophilin than plasma, owing to release of this protein from granulocytes during clotting. This phenomenon also changed the isoprotein pattern. Plasma and serum from newborn infants and from patients with leucocytosis, polycythaemia vera and chronic myelogenous leukaemia contained the same isoproteins as were found in plasma from healthy subjects. In addition to these, isoproteins with lower than 'normal' pI values were often found in chronic myelogenous leukaemia and occasionally in leucocytosis. It is concluded that cobalophilin from different fluids and cells is a single microheterogeneous protein with a variable carbohydrate composition. The distribution of cobalophilin in different body fluids and cells supports the suggestion that cobalophilin is an antimicrobial protein (Gullberg 1972) like lactoferrin and lysozyme.
D000653:Amniotic Fluid / Q000032:analysis; D001792:Blood Platelets / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D005750:Gastric Juice / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006098:Granulocytes / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn; D007951:Leukemia, Myeloid / Q000097:blood; D007964:Leukocytosis / Q000097:blood; D008895:Milk, Human / Q000032:analysis; D008970:Molecular Weight; D011087:Polycythemia Vera / Q000097:blood; D011485:Protein Binding; D012463:Saliva / Q000032:analysis; D014805:Vitamin B 12 / Q000032:analysis* / Q000097:blood
D001798:Blood Proteins; D006023:Glycoproteins; D014805:Vitamin B 12