Cytoenzymology and 3H-thymidine uptake of retro-ocular connective tissue cultures in experimental endocrino-exophthalmos.
The in vitro retro-ocular connective tissue cultures from guinea pigs with endocrine exophthalmos were studied before and after retro-ocular treatment with cortisol and hyaluronidase. Both cortisol and hyaluronidase inhibited the cell proliferation, the cytoenzymic activities of oxydoreductases, the 3H-thymidine uptake, the number of mitoses and the protein content of cultivated cells.
D000818:Animals; D003238:Connective Tissue / Q000201:enzymology / Q000378:metabolism*; D005094:Exophthalmos / Q000201:enzymology / Q000378:metabolism*; D005347:Fibroblasts; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006168:Guinea Pigs; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008940:Mitotic Index; D009915:Orbit; D010088:Oxidoreductases / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism*
D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D006821:Hyaluronoglucosaminidase; D013936:Thymidine; D006854:Hydrocortisone