Morphological characterisation of adult acute leukaemia in short-term liquid culture.
Peripheral blood leukocytes from 31 out of 48 patients with acute myelogenous leukemia grew in short-term liquid culture. Two distinct types of growth occurred. The first type was dimorphic with supernatant free-floating ("non-sticker") cells and, in addition, a plastic/glass adherent, trypsin resistant, phagocytic population ("stickers"). The second type which occurred less frequently than the first consisted almost entirely of free-floating "non-sticker" cells. Although patients with this second type of growth pattern almost invariably had AML, 44% of AML's produced monocytoid "sticker" cells in culture. Cells from the majority of patients with ALL did not grow in culture.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology*; D008875:Middle Aged; D013936:Thymidine / Q000378:metabolism
D003470:Culture Media; D013936:Thymidine