title: [Studies on the cytotoxicity of silver alloys be means of cell culture (author's transl)].
abstract: In order to investigate biocompatibility of silver alloys, which is widely used in Japan, L strain cells were brough contact with the alloys. Results were assessed by cell multiplication rate calculated from counting cell nuclei number per milliter of medium and morphological observation. The materials used were two from of silver-tin-zinc alloys, one form of silver-tin-zinc-cadmiumalloy and two forms of silver-copper alloys. Copper-zine alloy, which is reported to be highly cytotoxic, was alsoutilized as a positive control. Following results were obtained: 1) Ingot specimen of these alloys showed cytotoxicity, Silver-tin-zinc alloy, silver-tin-zinc-cadmium alloy and silver-copper alloy containing 10% or less of copper showed intense cytotoxicity initially, with diminishing cytotoxic action with time. On the otherhand, silver-copper alloy containing 20% or more of copper showed persistent cytotoxicity. Copper-zinc alloy showed intense cytotoxicity. 2) Cast specimen of these alloys showed increasing cytotoxicity with time campared to their ingot specimen. The results are seemed to owe much to inclusion of toxic substances from the alloy and investment material or alteration of surface characteristics of the alloys due to casting procedure. 3) Morphological observation did not differ from the results of cell nuclei counting. It is expected that the present results could give a clue on animal experiments or clinical use from the view point of biocompatibility of silver alloys.
mesh_terms: D001672:Biocompatible Materials; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology; D003722:Dental Alloys / Q000494:pharmacology*; D012834:Silver / Q000494:pharmacology*; D014001:Tin / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D003722:Dental Alloys; D012834:Silver; D014001:Tin; D003300:Copper; D015032:Zinc