title: Absorption cytophotometry: evaluation of three methods for the determination of DNA in Feulgen stained nuclei.
abstract: Three methods for the evaluation of the relative amount of DNA per nucleus by absorption cytophotometry are compared. A combination of the two-wavelength method (Patau, 1952; Ornstein, 1952), the one-wavelength two-area method (Garcia, 1965) and the determination of transmission through nuclear plugs is proposed in order to estimate systematic errors made by absorption cytophotometry.
mesh_terms: D004247:DNA / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006651:Histocytochemistry; D006868:Hydrolysis; D008433:Mathematics; D008962:Models, Theoretical; D013053:Spectrophotometry / Q000379:methods
chemical_list: D004247:DNA