Isolation of a cationic polypeptide from human serum that stimulates proliferation of 3T3 cells.
A basic polypeptide that stimulates DNA synthesis and cell division in confluent populations of mouse Balb/c-3T3 cells has been isolated from whole human serum, and has been separated from the heterogenous group of molecules with insulin-like activity. This highly purified basic polypeptide has a molecular weight of 1.3 x 10(4) and an isoelectric point of 9.7. Approximately 10(7) polypeptide molecules in the growth medium allow the replication of one density-inhibited cell.
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured / Q000502:physiology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D007525:Isoelectric Focusing; D008623:Mercaptoethanol; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D014357:Trypsin
D010455:Peptides; D008623:Mercaptoethanol; D002918:Chymotrypsin; D014357:Trypsin