[Differentiation of cytoplasmatic, mitochondrial and lysosomal enzymes by fractional extraction of rat liver].
Enzymes of extramitochondrial, mitochondrial and lysosomal origin were differentiated by the method of fractional tissue extraction from rat liver. It could be confirmed that soluble enzymes of the extramitochondrial (cytoplasmatic) compartment (glycerine aldehydphosphate-dehydrogenase, argininosuccinate lyase) and soluble enzymes of the mitochondrial matrix can be differentiated easily according to their different intracellular localisation. Additionally, our results emphasize that soluble enzymes of both compartements and marker-enzymes of the lysosomal cell space which are known to be structurally-bound, can be differentiated too. However, the method failed to establish significant differences between enzymes located in the submitochondrial compartment (adenylatekinase, monaminooxidase and succinic-dehydrogenase) and lysosomal enzymes (cathepsin-D and N-acetyl-D-glucosaminidase).
D000818:Animals; D001123:Argininosuccinate Lyase / Q000378:metabolism; D002403:Cathepsins / Q000378:metabolism; D002458:Cell Fractionation; D003593:Cytoplasm / Q000201:enzymology*; D005260:Female; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D051381:Rats; D013385:Succinate Dehydrogenase / Q000378:metabolism
D013385:Succinate Dehydrogenase; D002403:Cathepsins; D001123:Argininosuccinate Lyase
Fraktionierte Zellextraktion zur Differenzierung cytoplastischer, mitochondrialer und lysosomaler Enzyme der Leber.