Regulation of synthesis of hepatic fatty acid synthetase: polysomal translation in a cell-free system.
Polysomes were isolated from livers of rats fed various diets and were translated in a protein-synthesizing system derived from cultured Chang liver cells. One of the labeled products was identified as complete subunit(s) of fatty acid synthetase by indirect immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the solubilized immunoprecipitate. The relative amounts of fatty acid synthetase synthesized by polysomes from livers of rats fed a normal diet, starved rats, and rats starved and refed a fat-free diet for 16 hr were 1, 0.1, and 10, respectively. Induction of synthesis of fatty acid synthetase after fat-free refeeding of starved rats began by 2 hr (3-fold increase over starved animals), was increasing rapidly by 5 hr (19-fold over starved animals), and reached a high level by 16 hr (95-fold over starved and 10-fold over normally fed).
D000818:Animals; D000937:Antigen-Antibody Reactions; D002474:Cell-Free System; D064429:Fatty Acid Synthases / Q000096:biosynthesis* / Q000276:immunology; D008099:Liver / Q000201:enzymology*; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D013217:Starvation; D013997:Time Factors
D064429:Fatty Acid Synthases