[Modifications of ribosomes from rat liver with alkylating derivatives of tRNA].
Alkylating analogs of peptidyl-tRNA: N-chloroambucilyl-14C-phenylanalyl-tRNA (1), N-iodoacetyl-14C-phenylalanyl-tRNA (2) and N-bromo-acetyl-14C-phenlalanyl-tRNA (3) were applied for the modification of the peptidyl-transferase center of the 80S ribosomes from rat liver. These analogs, being in the teronary complex poly-U: ribosome : tRNA analog, modified ribosomal proteins and ribosomal RNA. The modification is directed to large ribosomal subunit. It is found, that (1) modifies ribosomal proteins L5, L25, L31 and L32 and (2) modifies ribosomal proteins L4, L6, L10+L11, L13 and L30.
D000818:Animals; D002699:Chlorambucil / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D010458:Peptidyl Transferases / Q000378:metabolism; D010649:Phenylalanine / Q000031:analogs & derivatives; D011072:Poly U / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism*; D051381:Rats; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*
D007461:Iodoacetates; D012269:Ribosomal Proteins; D002699:Chlorambucil; D011072:Poly U; D010649:Phenylalanine; D012343:RNA, Transfer; D010458:Peptidyl Transferases
Modifikatsiia ribosom iz pecheni krys s pomoshch'iu alkiliruiushchikh proizvodnykh tRNK.