title: Interaction of poly(A) and mRNA with eukaryotic initiator met-tRNA-f binding factor: identification of this activity on reticulocyte ribonucleic acid protein particles.
abstract: Messenger ribonucleoprotein particles (mRNPs) have been isolated from rabbit reticulocyte polysomes. One of the proteins of the mRNP complex has many properties of a specific eukaryotic initiation factor, the soluble Met-tRNA-f binding protein. A purified preparation of this factor, in addition to binding Met-tRNA-f, binds poly(A) and globin mRNA. The binding of these substrates is greater than that obtained with other natural or artificial polyribonucleotides. The mRNP fraction also binds poly(A) and Met-tRNA-f. Since the factor which binds initiator tRNA also binds poly(A) and mRNA, and this activity can be found on mRNPs, there may be a relationship between initiator tRNA binding and messenger binding in early events of eukaryotic initiation.
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D008715:Methionine; D009698:Nucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D010448:Peptide Initiation Factors; D011119:Polynucleotides / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000648:ultrastructure; D012270:Ribosomes / Q000032:analysis
chemical_list: D000227:Adenine Nucleotides; D009698:Nucleoproteins; D010448:Peptide Initiation Factors; D011119:Polynucleotides; D012333:RNA, Messenger; D012343:RNA, Transfer; D008715:Methionine