Human X-Linked genes regionally mapped utilizing X-autosome translocations and somatic cell hybrids.
Human genes coding for hypoxanthine phosphoribosyltransferase (HPRT, EC 2.4.2.8; IMP:pyrophosphate phosphoribosyltransferase), glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49; D-glucose-6-phosphate:NADP+ 1-oxidoreductase), and phosphoglycerate kinase (PGK, EC 2.7.2.3; ATP:3-phospho-D-glycerate 1-phosphotransferase) have been assigned to specific regions on the long arm of the X chromosome by somatic cell gentic techniques. Gene assignment and linear order were determined by employing human somatic cells possessing an X/9 translocation or an X/22 translocation in man-mouse cell hybridization studies. The X/9 translocation involved the majority of the X long arm translocated to chromosome 9 and the X/22 translocation involved the distal half of the X long arm translocated to 22. In each case these rearrangements appeared to be reciprocal. Concordant segregation of X-linked enzymes and segments of the X chromosome generated by the translocations indicated assignment of the PGK gene to a proximal long arm region (q12-q22) and the HPRT and G6PD genes to the distal half (q22-qter) of the X long arm. Further evidence suggests a gene order on the X long arm of centromere-PGK-HPRT-G6PD.
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D004592:Electrophoresis, Starch Gel; D005347:Fibroblasts / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology / Q000648:ultrastructure*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis; D007621:Karyotyping; D007962:Leukocytes / Q000201:enzymology; D051379:Mice; D010735:Phosphoglycerate Kinase / Q000032:analysis; D012641:Selection, Genetic; D012730:Sex Chromosomes
D005954:Glucosephosphate Dehydrogenase; D007041:Hypoxanthine Phosphoribosyltransferase; D010735:Phosphoglycerate Kinase