title: Biochemical and morphological studies of rat submandibular gland: I. Centrifugal fractionation of granule-rich fraction.
abstract: Submandibular glands from male rats were homogenized in 0.34 M sucrose and 0.5 mM ethylenediaminetetraacetic acid in 10 mM HEPES buffer at a pH of 7.4. The extract was centrifuged and filtered through nuclepore filters to prepare a granule-rich fraction. Electron dense zymogen granules constituted approximately 85% of the particles in this fraction which also contained about a third of the total alkaline esterase activity in the gland.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000468:Alkalies; D000818:Animals; D002498:Centrifugation; D003594:Cytoplasmic Granules / Q000032:analysis / Q000201:enzymology / Q000648:ultrastructure; D004950:Esterases / Q000378:metabolism; D004952:Esters; D008297:Male; D008854:Microscopy, Electron; D010447:Peptide Hydrolases / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D013363:Submandibular Gland / Q000201:enzymology / Q000648:ultrastructure*
chemical_list: D000468:Alkalies; D004952:Esters; D004950:Esterases; D000135:Acid Phosphatase; D010447:Peptide Hydrolases