Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes.
Rabbit globin poly(A)-free and native mRNA preparations were microinjected into Xenopus oocytes. The amount of globin message remaining after incubation of injected oocytes was determined by molecular hybridization with a radioactive complementary DNA probe, synthesized by copying globin mRNA with purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus. The results show that 56 hr after injection about 85% of the poly(A)-free mRNA molecules are degraded, while native poly(A)-containing mRNA chains are almost completely preserved during the same period of time.
D000818:Animals; D005260:Female; D005914:Globins / Q000096:biosynthesis; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D011061:Poly A / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D014981:Xenopus
D012333:RNA, Messenger; D011061:Poly A; D005914:Globins