Lipoate effect on carbohydrate and lipid metabolism and gastric H+ secretion.
Acid secretion (QH+) and oxygen consumption (Qo2) by frog gastric mucosae in vitro were sharply stimulated by lipoate. A rapid decline followed stimulation, subsequently falling below control values. Addition of only glucose or lactate had no effect on Qo2 or QH+. Pyruvate caused slight significant stimulation of Qo2. Any one of these compounds added to lipoate-treated mucosae increased the stimulatory effect of lipoate and markedly slowed the rate of decline subsequent to maximum stimulation. Various fatty acids had a moderate-to-high stimulatory effect on Qo2 and QH+. Lipoate added prior to the addition of fatty acids decreased the stimulatory effect of buryrate (minus 56%), decanoate (minus 87%), and palmitate (minus 60%). Propionate became an inhibitor in the presence of lipoate. Lipoate increased (plus 100%) the amount of glycogen oxidized and decreased (minus 69%) the amount of triglycerides oxidized. Lipoate-treated mucosae did not respond to histamine. Addition of glucose restored responsiveness. The results indicate that beta-oxidation of fatty acids plays a major role in the acid secretory process and is centrally involved in cyclic AMP and histamine stimulation of QH+.
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D003652:Decanoic Acids / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D005750:Gastric Juice / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007773:Lactates / Q000494:pharmacology; D050356:Lipid Metabolism; D010101:Oxygen Consumption / Q000187:drug effects*; D010169:Palmitic Acids / Q000494:pharmacology; D011422:Propionates / Q000494:pharmacology; D011773:Pyruvates / Q000494:pharmacology; D011892:Rana catesbeiana; D013268:Stimulation, Chemical; D013831:Thiamine / Q000494:pharmacology; D013835:Thiamine Pyrophosphate / Q000494:pharmacology; D008063:Thioctic Acid / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
D002087:Butyrates; D003652:Decanoic Acids; D005227:Fatty Acids; D007773:Lactates; D010169:Palmitic Acids; D011422:Propionates; D011773:Pyruvates; D014280:Triglycerides; D008063:Thioctic Acid; D006632:Histamine; D006003:Glycogen; D005947:Glucose; D013835:Thiamine Pyrophosphate; D013831:Thiamine