[Role of DNA-membrane attachment in repair of radiation damage in Micrococcus radiodurans].
The unirradiated bacterial DNA assciated with the membrane is liberated into the cytoplasm after breakage of either a single or a double strand, resulting from X-ray action. During the reincubation period in growth-medium, the DNA is reassociated with the membrane. This phenomenon is very rapid and occurs without increasing the molecular weight of DNA. The study of DNA-membrane complexes shows that the size of the DNA-associated membranous fragment differs according to the lysing technique employed, appearing as a change in the density of the complex. Chloramphenicol decreases reassociation, and iodoacetamide, a radiosensititzing agent, inhibits it completely.
D000081:Acetamides / Q000494:pharmacology; D001665:Binding Sites; D002701:Chloramphenicol / Q000494:pharmacology; D004260:DNA Repair; D004269:DNA, Bacterial; D008566:Membranes; D008837:Micrococcus / Q000528:radiation effects*; D011830:Radiation Effects; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D013936:Thymidine; D014316:Tritium; D014965:X-Rays
D000081:Acetamides; D004269:DNA, Bacterial; D011838:Radiation-Sensitizing Agents; D014316:Tritium; D002701:Chloramphenicol; D013936:Thymidine
Rôle de l'attachement du DNA á la membrane dans la réparation des radiolésions chez Micrococcus radiodurans