title: Hair dyes are mutagenic: identification of a variety of mutagenic ingredients.
abstract: We have previously described a sensitive bacterial test for dectecting carcinogens as mutagens. We have previously described a sensitive bacterial test for detecting carcinogens as mutagens. We show here that 89% (150/169) of commercial oxidative-type (hydrogen peroxide) hair dye formulations are mutagenic in this test. Of the 18 components of these hair dyes, nine show various degrees of mutagenicity:2,4-diaminoanisole, 4-nitro-o-phenylenediamine,  2-nitro-p-phenylenediamine, 2,5-diaminoanisole, 2-amino-5-nitrophenol, m-phenylenediamine,  o-phenylenediamine,  2-amino-4-nitrophenol, and 2,5-diaminotoluene. Three hair dye components (p-phenylenediamine, 2,5-diaminotuluene, and 2,5-diaminoanisole) become strongly mutagenic after oxidation by H2O2: the mutagenic product of p-phenylenediamine is identified as the known trimer, Bandrowski's base. 2,4-Diaminotoluene, a hair dye component until recently, is also shown to be mutagenic: this compound has been shown to be a carcinogen in rats and is used in large amounts in the polyurethane foam industry. About 20,000,000 people (mostly women) dye their hair in the U.S. and the hazard could be considerable if these chemicals are actually mutagenic and carcinogenic in humans.
mesh_terms: D000588:Amines / Q000494:pharmacology; D000840:Anisoles / Q000494:pharmacology; D004396:Coloring Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006197:Hair; D006861:Hydrogen Peroxide; D009153:Mutagens / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010655:Phenylenediamines / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects*
chemical_list: D000588:Amines; D000840:Anisoles; D004396:Coloring Agents; D009153:Mutagens; D010655:Phenylenediamines; D006861:Hydrogen Peroxide