Binding of poly (Glu-60 Ala-30 Tyr-10) by thymic lymphocytes from genetic responder and non-responder mice: effect of antihistocompatibility serum.
The binding of 125-I-labeled GAT by murine thymocytes was studied by autoradiography. GAT binding was highly temperature dependent. Significantly more thymocytes bound GAT at 37 degrees C than at 4 degrees C. GAT bound at 37 degrees C was usually distributed in a "cap" over one pole of the cell. Preincubation of thymocytes with specific anti-H-2 serum markedly inhibited the subsequent binding of GAT. The frequency of antigen-binding thymocytes was not significantly affected by preincubation of thymocytes with anti-Thy-1 serum or a number of anti-immunoglobulin sera. The immune response to GAT is controlled by an H-2 linked immune response gene(s). Mice of strains that are H-2p, H-2q or H-2s (non-responders) fail to develop effective T cell helper function necessary for a humoral immune response to GAT under most conditions. However, both responder and non-responder strains had similar numbers of thymocytes capable of binding GAT in vitro, indicating that T cells from non-responder strains of mice do possess a recognition mechanism for GAT.
D000409:Alanine; D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D001345:Autoradiography; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology; D005971:Glutamates; D006649:Histocompatibility Antigens; D007106:Immune Sera / Q000494:pharmacology; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D011817:Rabbits / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology; D014443:Tyrosine
D000941:Antigens; D005971:Glutamates; D006649:Histocompatibility Antigens; D007106:Immune Sera; D007457:Iodine Radioisotopes; D014443:Tyrosine; D000409:Alanine