Polyene sensitivity during germination of conidia of Aspergillus fumigatus.
A system for the rapid and relatively synchromous germination of conidia from a clinical isolate of Aspergillus fumigatus is described. The polyene-mediated release of K plus from germinating conidia has been determined. Ungerminated conidia were insensitive to amphotericin B methyl ester (AME) at concentrations greater than 50 mug/ml, but rapidly became sensitive to 1 to 2 mug AME/ml during the intitial stages of germination. These findings have been correlated with minimum inhibitory concentration values obtained in studies of conidial germination and hyphal outgrowth using a variety of growth tests.
D000666:Amphotericin B / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors
D000666:Amphotericin B; D011188:Potassium