Effects of anti-Ia sera on mitogenic responses. II. Differential expression of the Ia marker on phytohemagglutinin and concanavalin A-reactive T cells.
Genes mapping in the I region of the H-2 complex control a system of lymphocyte alloantigens (Ia) which are expressed on a subpopulation of T cells and on most B cells. Specific anti-Ia serum in the presence of rabbit complement removed the splenic T-cell subpopulation responsive to Con-A, but did not affect the response to phytohemagglutinin (PHA) or Leucoagglutinin. Antibodies specific for Ia, H-2K, or H-2D membrane antigens were used without complement to pretreat spleen cells. These antibody pretreated cells responded normally to Con-A and PHA.
D000818:Animals; D000918:Antibody Specificity; D002462:Cell Membrane / Q000276:immunology; D002874:Chromosome Mapping; D003165:Complement System Proteins; D003208:Concanavalin A / Q000494:pharmacology*; D005796:Genes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007106:Immune Sera / Q000494:pharmacology*; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007106:Immune Sera; D037102:Lectins; D003208:Concanavalin A; D003165:Complement System Proteins