[Use of peritoneal macrophages labeled with Cr51 and cultivated in vitro as target cells for quantitative assessment of the cytotoxic activity of immune T-lymphocytes].
Peritoneal macrophages previously labeled in a suspension with 51Cr and grown in the form of a discontinuous monolayer on glass, pretreated with poly-l-lysin were used as target cells. This permitted to estimate the cytotoxic activity of immune lymphocytes by 51Cr release for the period of 4 and 20 hours of incubation with the target. 51Cr release from such a target incubated with normal lymphocytes for 20 hours did not exceed 10-20% of the maximum release. Application of a 2% solution of sodium dodecylsulfate ensured a 100 per cent solubilization of labeled macrophages growing on the glass surface, and permitted the cytotoxic effect to be determined by measuring the label remaining in the intact cells.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003470:Culture Media; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D008239:Lysine; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D011108:Polymers; D012967:Sodium Dodecyl Sulfate; D013601:T-Lymphocytes / Q000276:immunology*
D002860:Chromium Radioisotopes; D003470:Culture Media; D011108:Polymers; D012967:Sodium Dodecyl Sulfate; D008239:Lysine
Ispol'zovanie peritoneal'nykh makrofagov, mechennykh Cr51 i kul'tivirovannykh in vitro, v kachestve kletok-mishenei dlia kolichestvennoĭ otsenki tsitotoksicheskoĭ aktivnosti immunykh T-limfotsitov