title: Kuhnt intermediary tissue as a barrier between the optic nerve and retina.
abstract: Utilizing adult albino rats and lanthanum nitrate as a tracer, electron microscopic studies were done to provide additional information on the blood-optic nerve barrier. Following injection into the cervical artery, lanthanum was observed to fill the intercellular space of the optic nerve parenchyma. Diffusion from the prelaminar optic nerve into the juxta-optic nerve retina was, however, prevented by the Kuhnt tissue at the level of the rod and cone layer. In this region, tight junctions were found between the glial cells of the Kuhnt intermediary tissue and were continuous from the tight junctions of the retinal pigment epithelial cells to the junctions (zonulae adhaerentes) of the outer limiting membrane. From the level of the outer limiting membrane to the inner limiting membrane there was no diffusion of lanthanum into the adjacent retina despite the absence of tight junctions, and the lanthanum which had diffused from the choroid was never observed in the area of the inner limiting membrane of the optic nerve head. It is in this region that the functional barrier may exist.
mesh_terms: D000818:Animals; D001692:Biological Transport; D001769:Blood; D004058:Diffusion; D007365:Intercellular Junctions / Q000648:ultrastructure; D007811:Lanthanum; D008854:Microscopy, Electron; D009457:Neuroglia / Q000378:metabolism* / Q000648:ultrastructure; D009900:Optic Nerve / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D012160:Retina / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D007811:Lanthanum