Differential sensitivity to x-ray of chromosomes of blood T-lymphocytes and B-and T-cell lines.
Normal T-lymphocytes, B-cell line (CCRF-SB) and T-cell line (CCRF-HSB-2) cells, all diploid in their chromosome constitution, were exposed in vitro to various doses of X-ray and analyzed at their first mitotic division for structural chromosome abnormalities. The irradiation effects were determined also by a viability test of the cells, using trypan blue dye. The irradiated T-cell line (CCRF-HSB-2) showed a remarkably high frequency of chromosome aberrations, including chromosome and chromatid deletions, chromatid exchanges, dicentrics, rings and acentric fragments. On the other hand, the chromosome aberrations observed in the irradiated B-cell line and normal T-lymphocytes consisted mainly of dicentrics, rings, deletions and acentric fragments; the frequency of chromosome and chromatid deletions was low as compared to that of the T-cell line. The cell viability test showed a singificantly higher percent reduction of viable cells at every dose of X-ray in the irradiated T-cell line than in the B-cell line or the normal T-lymphocytes. It is possible that the increased radiosensitivity of the T-cell line is related to the original malignant nature of the cells, which originated from the lymphocytes of a patient with acute lymphoblastic leukemia.
D001402:B-Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D002460:Cell Line; D002470:Cell Survival; D002842:Chromatids / Q000502:physiology; D002872:Chromosome Deletion; D002875:Chromosomes / Q000528:radiation effects* / Q000648:ultrastructure; D003434:Crossing Over, Genetic; D004307:Dose-Response Relationship, Radiation; D007621:Karyotyping; D013601:T-Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D014178:Translocation, Genetic; D014965:X-Rays