T Cells in immunodeficiencies as evaluated by an antihuman T-cell serum.
An antihuman T-cell has been prepared in rabbits by infecting lymphocytes from a patient with Bruton-type agammaglobulinemia and absorption with cultured B-lymphoblast cells. Cytotoxicity of the antiserum was assayed with peripheral blood lymphocytes in the presence of rabbit complement and its specificity for T vs B cells was verified. This anti-T-cell serum killed 66% (range 49-78) of normal blood lymphocytes. In patients with Bruton-type agammaglobulinemia the percentage of cells sensitive to the antiserum was above normal (77-89%), whereas it was decreased in some patients with lepromatous leprosy or under antilymphocyte globulin therapy.
D000042:Absorption; D000293:Adolescent; D000361:Agammaglobulinemia / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D000961:Antilymphocyte Serum; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D005374:Filtration; D005455:Fluorescent Antibody Technique; D006041:Goats / Q000276:immunology; D003368:Gossypium; D006649:Histocompatibility Antigens; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D007518:Isoantibodies / Q000032:analysis; D008297:Male; D009000:Monocytes; D011817:Rabbits / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D006649:Histocompatibility Antigens; D007518:Isoantibodies