Sensitive methods for the detection and characterization of double helical ribonucleic acid.
We have evaluated three methods which respond specifically to stable RNA-RNA duplexes and have compared their utility for examining several sorts of nucleic acids. We find that these methods, stepwise chromatography on Whatman CF11-cellulose; digestion with Escherichia coli RNase III; and specific inhibition of globin synthesis in vitro in rabbit reticulocyte lysates, are able to distinguish between stable double-stranded RNA and single-stranded RNA in the expected manner. The most sensitive method, inhibition of globin synthesis, responds to double-stranded RNA concentrations below 0.1 ng per ml. We have used the predominantly single-stranded RNA from several RNA bacteriophages of E. coli to test both the sensitivity and selectivity of these methods. The three viral RNAs tested contain low levels of double-stranded RNA which can be readily removed, leaving RNA which is not recognized as double-stranded RNA, despite indications from physical and sequencing studies that secondary structure is present. In particular, a potential hairpir loop of known sequence has been isolated from phage f2 RNA. Its properties were found to depart significantly from those of RNA-RNA duplexes by those two of our three methods capable of testing RNA of this size. Analysis of two eukaryotic mRNA populations by these methods was complicated by the presence of poly(A). Synthetic poly(A) chromatographs like double-stranded RNA on cellulose CF11 columns, and we could distinguish it from reovirus double-stranded RNA only at elevated temperatures.
D000818:Animals; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D004926:Escherichia coli / Q000201:enzymology; D005914:Globins / Q000096:biosynthesis; D008722:Methods; D008832:Microchemistry; D011119:Polynucleotides; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012260:Ribonucleases
D011119:Polynucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D005914:Globins; D012260:Ribonucleases