Effect of alloxan on permeability and hexose transport in rat pancreatic islets.
The in vitro effect of alloxan exposure on the permeability of collagenase isolated rat pancreatic islets to sucrose, D-mannitol, and L-glucose has been investigated. Determination of changes in cell volume with a non-wash double label isotope procedure indicates that alloxan treatment exerts no measurable effect on permeability to sucrose, D-mannitol, or L-glucose as compared to nonalloxan-treated islets. In addition, neither prior exposure nor the concomitant presence of alloxan alters the rate of D-glucose or 3-0-methyl-D-glucose transport into rat pancreatic islets. It is concluded that the in vitro effect of alloxan on abolishing glucose-induced insulin release in isolated rat pancreatic islets does not appear to be the result of permeability changes to small organic molecules or alteration in the transport of D-glucose into the beta-cell.
D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002250:Carbon Radioisotopes; D002463:Cell Membrane Permeability; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006601:Hexoses / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008353:Mannitol / Q000378:metabolism; D051381:Rats; D013395:Sucrose / Q000378:metabolism; D014316:Tritium; D014508:Urea / Q000378:metabolism
D002250:Carbon Radioisotopes; D006601:Hexoses; D007328:Insulin; D014316:Tritium; D008353:Mannitol; D013395:Sucrose; D000496:Alloxan; D014508:Urea; D005947:Glucose