Isolation of alpha1-antitrypsin in a new preparative electrophoresis apparatus.
The construction and operation of an apparatus is described which enables alpha1-antitrypsin to be isolated from human serum by preparative electrophoresis in polyacrylamide gel. The initial pass through the chamber yields a fraction that is predominantly albumin and several alpha1-proteins. After removal of albumin by affinity chromatography, a second pass through the chamber separates the individual alpha1-proteins. A volume of 50 ml of serum may be accommodated by the chamber, and the recovery of activity in each step is greater than 60%. The entire procedure may be completed in 36 hrs.
D002846:Chromatography, Affinity; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D000515:alpha 1-Antitrypsin / Q000302:isolation & purification* / Q000378:metabolism
D000515:alpha 1-Antitrypsin; D003208:Concanavalin A