title: Cellular cooperation during in vivo anti-hapten antibody responses. I. The effect of cell number on the response.
abstract: Cellular interactions in adoptive secondary anti-hapten antibody responses to the hapten 2, 4-dinitrophenyl (DNP) have been studied. It was shown that DNP-specific B cells must interact with carrier specific helper T cells to give optimal responses. Independent titration of B cell and helper cell activity in adoptive anti-DNP antibody responses gave the following results: Doubling the number of transferred B cells approximately doubled the subsequent antibody response. Doubling the number of helper cells leads to nearly 4 times as much anti-DNP antibody, measured 7 days after boosting ("premium effect"). This marked effect of helper cell number on the antibody response is thought to be due primarly to the interaction of two populations of carrier-specific cells in the helper effect, or to the interaction of two activities of a single population of helper cells, namely clone activation and clone expansion. Only a very small proportion of the premium effect given by helper cells could be attributed to increases in antibody affinity.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000918:Antibody Specificity; D000961:Antilymphocyte Serum; D001386:Azides; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D006241:Haptens; D006433:Hemocyanins; D007111:Immunity, Cellular; D007114:Immunization; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008808:Mice, Inbred CBA; D010047:Ovalbumin; D011828:Radiation Chimera; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology / Q000637:transplantation; D014184:Transplantation, Homologous
chemical_list: D000906:Antibodies; D000961:Antilymphocyte Serum; D001386:Azides; D006241:Haptens; D010047:Ovalbumin; D006433:Hemocyanins