Studies of mouse-brain-associated T-lymphocyte antigens.
Xeno- and alloantigens shared by mouse brain, thymocytes and thymus-derived lymphocytes were isolated from brain by butanol extraction, followed by Sephadex G-200 column chromatography and preparative polyacrylamide gel electrophoresis, both in the presence of SDS. The antigenic activity, as tested by inhibition of complement-mediated cytotoxicity using a xenogeneic antiserum directed against T cell-specific antigens (anti-MTLA), was enriched 50-fold during the purification procedure. Xenogeneic and allogeneic antisera which were specific for mouse T-lymphocytes, could be obtained by immunization of rabbits and mice with the partially purified antigens.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000302:isolation & purification*; D001921:Brain / Q000276:immunology*; D002850:Chromatography, Gel; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D000941:Antigens