Effect of aeration on minimal medium recovery of heated Salmonella typhimurium.
The effect of presence or absence of air on minimal medium recovery of heated Salmonella typhimurium was investigated. It was determined that the expression of minimal medium recovery is not only dependent on heat and a nutritionally complex medium but also on air. Unlike in the presence of air, in the presence of nitrogen, cells were able to recover their ability to grow on Trypticase soy agar enriched with 0.5% yeast extract (TSY) when incubated in TSY broth. It was established that in the presence of nitrogen the number of heat-TSY- induced, single-straneded breaks in deoxyribonucleic acid (DNA) were less than in the presence of air. Furthermore, the DNA breaks in nitrogen were repaired, whereas DNA breaks in air were not. The ability of cells to grow on TSY agar corresponded well with their ability to repair damage to DNA.
D000362:Agar; D000388:Air; D000693:Anaerobiosis; D002452:Cell Count; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D004260:DNA Repair; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis; D006358:Hot Temperature; D008970:Molecular Weight; D009584:Nitrogen; D012486:Salmonella typhimurium / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D004269:DNA, Bacterial; D000362:Agar; D009584:Nitrogen