Antigenic analysis of the lymphomata.
Soluble protein extracts of non-Hodgkin's lymphomata are fractionated by Sepharose chromatography. Each protein segregate is tested as a target antigen in immunoelectrophoresis using antiserum prepared by immunization with each fraction and an F and S antiserum of Hodgkin's tumour. F and S antigens are prominent in a histiocytic lymphoma with lymphoid infiltrate and Hodgkin's disease. A monospecific F antiserum is used to determine the stromal distribution of the antigen by immunofluorescence in tumour infiltrates and also to demonstrate F antigen activity in the medulla of the adolescent thymus. Implications of these findings are discussed and a general plan for analysis of the lymphomata outlined.
D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006689:Hodgkin Disease / Q000276:immunology; D006801:Humans; D007122:Immunoelectrophoresis; D008223:Lymphoma / Q000276:immunology*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000276:immunology; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D000951:Antigens, Neoplasm