[Production of monospecific serum against secretory IgA (anti-SC) and a standard for quantitative analysis].
The authors describe a method of obtaining monospecific serum against secretory IgA and the corresponding standard. An immunochemically pure (11.6S) secretory human IgA was extracted from the colostrum by salt fractionation and gel-filtration through Sephadex G-200 and Sepharose 6B; this IgA was used as an antigen for the immunization and the standard for the quantitative determination of SIgA in the secretions. Monospecific anti-SC-serum was obtained by successive exhaustion of the antiserum against the S IgA immunosorbents prepared from normal human serum and the serum of a patient suffering from A myeloma containing polymeric IgA forms.
D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification; D002850:Chromatography, Gel; D003126:Colostrum / Q000276:immunology; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000302:isolation & purification; D011247:Pregnancy
D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A
Poluchenie monospetsificheskoĭ syvorotki protiv sekretornogo IgA (anti-SC) i standarta dlia kolichestvennogo analize