The isolation of two different lipopolysaccharide fractions from various Proteus mirabilis strains.
Four distinct Proteus mirabilis strains were extracted by the phenol/water procedure. After ultracentrifugation of the dialyzed water phase, the pelleted lipopolysaccharide was purified and analyzed. The sugar composition of this lipopolysaccharide fraction I was similar for all four strains, containing only small amounts of strain-specific constituents. A second lipopolysaccharide fraction was isolated from the supernatant above (termed L1 fraction) after removal of nucleic acids. DEAE-cellulose chromatography indicated that this material is not a polysaccharide but rather a water-soluble lipopolysaccharide containing strain-specific constituents such as uronic acids, amino acids, amino sugars, neutral sugars, ethanolamine and phosphate, depending on the strain from which lipopolysaccharide II was isolated.
D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D008070:Lipopolysaccharides / Q000032:analysis / Q000302:isolation & purification*; D011513:Proteus mirabilis / Q000032:analysis*
D000596:Amino Acids; D000606:Amino Sugars; D008070:Lipopolysaccharides