The kinetic of degradation of chondroitin of sulphates and hyaluronic acid by chondroitinase form Proteus vulgaris.
Km and Vmax. were determined for the degradation by chondroitinase of chondroitin 4-sulphate, 4-sulphate-proteoglycna, chondroitin 6-sulphate, dermatan sulphate and hyaluronic acid. Degradation of chondroitin 4-sulphate was inhibited by hyaluronic acid but not by keratan sulphate. The results are discussed with regard to the use to the use of chondroitinase as a sleective reagent for the degradation of tissue glycosaminoglycans.
D002807:Chondroitin; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D007700:Kinetics; D008190:Lyases; D011509:Proteoglycans; D011514:Proteus vulgaris / Q000201:enzymology*
D011509:Proteoglycans; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D002807:Chondroitin; D008190:Lyases