Impaired T-cell transformation in chronic lymphocytic leukemia (CLL): Demonstration of a blastogenesis inhibitory factor.
A noncytotoxic serum factor was found in CLL serum which interfered with the response of normal lymphocytes to phytohemagglutinin (PHA) and pokeweed mitogen (PWM). This factor was not produced by B-lymphocytes during short-term culture. This factor may contribute to the impaired lymphocyte responsiveness in CLL. In five of six patients, T-lymphocytes which were purified by passage over nylon wool columns exhibited an impaired response to both PHA and PWM, suggesting that a dilutional effect alone does not fully explain the impaired lymphocyte transformation in CLL.
D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000097:blood / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D008934:Mitogens; D008940:Mitotic Index; D013601:T-Lymphocytes / Q000276:immunology*
D037102:Lectins; D008934:Mitogens