title: Subcellular fractions from dermis and epidermis in contact sensitization of guinea pigs to 1-chloro-2,4-dinitrobenzene.
abstract: Subcellular fractions were prepared from the epidermis of guinea pigs which had been painted with 1-chloro-2,4 dinitrobenzene 14 h previously. The microsomal fraction showed less activity than other fractions in the MIF test. Similarly, the microsomal fraction from the dermis of skin irritated with croton oil before DNCB painting was relatively inactive. However, the microsomal fractions from the epidermis and more especially from the dermis of skin painted with DNCB while undergoing allergic contact dermatitis from pentadecylcatechol showed significant MIF activities.
mesh_terms: D000473:Alkanes / Q000276:immunology; D000818:Animals; D002250:Carbon Radioisotopes; D002396:Catechols / Q000031:analogs & derivatives / Q000276:immunology; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology; D002464:Cell Migration Inhibition; D002713:Chlorine; D003436:Croton Oil / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology*; D000431:Ethanol / Q000276:immunology; D006168:Guinea Pigs; D008247:Lysosomes / Q000276:immunology; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis; D008861:Microsomes / Q000276:immunology; D009578:Nitrobenzenes / Q000276:immunology*; D009708:Nucleotidases / Q000378:metabolism; D012867:Skin / Q000166:cytology* / Q000276:immunology; D013347:Subcellular Fractions
chemical_list: D000473:Alkanes; D002250:Carbon Radioisotopes; D002396:Catechols; D008263:Macrophage Migration-Inhibitory Factors; D009578:Nitrobenzenes; D000431:Ethanol; D002713:Chlorine; D003436:Croton Oil; D009708:Nucleotidases