Growth of a capsid mutant of bacteriophage phi X174 in a temperature-sensitive strain of Escherichia coli.
A capsid mutant of phiX174 is capable of forming replicative form and synthesizing single strands at the restrictive temperature in a dnaB mutant of Escherichia coli. Under similar conditions, the wild-type bacteriophage is incapable of either step in viral synthesis.
D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000254:growth & development* / Q000378:metabolism; D004261:DNA Replication; D004267:DNA Viruses; D004269:DNA, Bacterial / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism*; D005796:Genes; D006358:Hot Temperature; D007106:Immune Sera; D009154:Mutation; D013696:Temperature; D013941:Thymine / Q000378:metabolism; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication
D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D004279:DNA, Viral; D007106:Immune Sera; D014764:Viral Proteins; D014316:Tritium; D013941:Thymine