An improved technique for obtaining E rosettes with human lymphocytes and its use for B cell purification.
The standard E rosette method and two previously described methods claimed to give improved E rosetting for enumeration of human T lymphocytes have been compared with respect to the speed of rosette formation, and the mechanical stability of the rosettes formed. Following rosette formation with the three methods, the lymphocyte-erythrocyte suspensions were sedimented on Ficoll-Isopaque to deplete them of rosette-forming cells and red cells. The purified lymphocyte preparations were tested for B and T cell markers to determine the degree of contamination with T cells. One of the improved rosetting methods was clearly better than the others, and led to the recovery of B lymphocytes with a contamination of only 2.0+/-1.9 per cent of T lymphocytes.
D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D006801:Humans; D007104:Immune Adherence Reaction / Q000379:methods*; D008264:Macrophages / Q000276:immunology; D010586:Phagocytes / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D011947:Receptors, Antigen, B-Cell