title: Progress in the resolution of the cytoplasmic membrane DNA initiation complex of Escherichia coli.
abstract: The attachment of the bacterial chromosome to the cytoplasmic membrane in Escherichia coli was studied. The initiator DNA was specifically labeled and the outer and cytoplasmic membranes were separated in a step sucrose gradient. The labeled DNA was localized mainly in the cytoplasmic membrane fraction. The DNA . cytoplasmic membrane complex was isolated from cells uniformly labeled with [Me-3H]thymidine, solubilized with deoxycholate and chromatographed on Sepharose 4B. A high percent of the labeled DNA was excluded in the void volume but a small fraction eluted associated with the second protein elution peak. The isolation of such a DNA . cytoplasmic membrane protein complex, suggests useful strategies for future studies about the molecular components of the initiation complex in E. coli.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002876:Chromosomes, Bacterial / Q000378:metabolism; D004261:DNA Replication; D004926:Escherichia coli / Q000378:metabolism*; D009154:Mutation; D011485:Protein Binding; D013385:Succinate Dehydrogenase / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D013385:Succinate Dehydrogenase