Affinity-labelling of Escherichia coli ribosomes by a derivative of phenylalanyl-tRNA. A critical test for the specificity.
Under nonenzymatic binding conditions p-nitrophenoxycarbonyl-phenylalanyl-tRNA can be bound to the donor as well as the acceptor site on the Escherichia coli ribosome. Precharging ribosomes with phenylalanyl-tRNA in the presence of elongation factor Tu and GTP blocks both the donor- and the acceptor site against affinity labelling by p-nitrophenoxycarbonyl-phenylalanyl-tRNA.
D000345:Affinity Labels; D002250:Carbon Radioisotopes; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D007553:Isotope Labeling; D008274:Magnesium / Q000494:pharmacology; D009578:Nitrobenzenes / Q000378:metabolism; D010445:Peptide Elongation Factors; D010649:Phenylalanine / Q000031:analogs & derivatives; D012343:RNA, Transfer / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000096:biosynthesis; D012270:Ribosomes / Q000378:metabolism*; D014316:Tritium
D000345:Affinity Labels; D002250:Carbon Radioisotopes; D009578:Nitrobenzenes; D010445:Peptide Elongation Factors; D012269:Ribosomal Proteins; D014316:Tritium; D010649:Phenylalanine; D012343:RNA, Transfer; D008274:Magnesium