Particulate matter in reconstituted amphotericin B and assay of filtered solutions of amphotericin B.
Amphotericin B colloidal solutions were prepared according to the manufacturer's directions and filtered with membrane filters of different porosity to test for particulate matter; and the concentration and in vitro microbiological activity of filtered and nonfiltered solutions were determined.  The amphotericin B solutions contained numerous particles.  Filtration through 0.85- and 0.45-mum filters did not reduce the in vitro antimicrobial activity, and filtration through 5-, 0.45-, and 0.22-mum filters did not alter the concentration of the drug when assayed spectrophotometrically.
D000666:Amphotericin B / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D003102:Colloids; D004340:Drug Contamination; D005374:Filtration; D010316:Particle Size; D013997:Time Factors
D003102:Colloids; D000666:Amphotericin B