Phospholipase activity plays no role in the action of colicin K.
1. A mutant lacking both detergent-resistant and detergent-sensitive phospholipase A activities is fully sensitive to colicin  K. 2. In the absence of cellular phospholipases A, colicin K does not promote hydrolysis of phosphatidylethanolamine. 3. Cells of the colicin-treated mutant lacking lysophosphatidylethanolamine are as abnormally permeable to Co-2+ as the wind type is. 4. Increased levels of lysophosphatidylethanolamine in colicin-treated cells are not necessary for the increased sensitivity to sodium dodecylsulfate.
D001692:Biological Transport; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003035:Cobalt / Q000378:metabolism; D003087:Colicins / Q000494:pharmacology*; D003902:Detergents / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013045:Species Specificity; D013997:Time Factors
D003087:Colicins; D003902:Detergents; D010714:Phosphatidylethanolamines; D012967:Sodium Dodecyl Sulfate; D003035:Cobalt; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D010740:Phospholipases