13C-enriched phosphatidylethanolamines from Escherichia coli.
Escherichia coli have been grown using [1-13C]acetate and [2-13C]acetate as the sole carbon source. The 13C NMR spectra of the whole cells and spheroplasts can be readily obtained but give limited information. The 13C NMR spectra of t-e isolated 13C-enriched phosphatidylethanolamines are assigned and analysed to give the biosynthetic pathway for acetate incorporation. This method provides a ready source of 13C-enriched phospholipids.
D000085:Acetates / Q000378:metabolism; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010714:Phosphatidylethanolamines / Q000096:biosynthesis*; D013104:Spheroplasts / Q000378:metabolism
D000085:Acetates; D002247:Carbon Isotopes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines