Stimulatory effects of 5-lodo-2'-deoxyuridine on number and function of splenic B- and T-cells and of macrophages in C3HeB/FeJ mice.
5-lodo-2'-deoxyuridine (lUdR) significantly increases the total number of spleen cells on the fourth day after administration of 100 mg/kg in normal C3HeB/FeJ male mice. The number of splenic B-cells [cells sensitive to the cytotoxic activity of a rabbit antiserum to mouse F(ab)2, plus complement] increases on the same day. The number of T-cells (cells sensitive to the cytotoxic activity of a rabbit antiserum to mouse brain-associated theta antigen, plus complement) and of macrophages (adherent-phagocytic cells) does not increase. Spleen cells from lUdR-treated mice show heightened responsiveness in vitro to Escherichia coli lipopolysaccharide or concanavalin A on Day 4 and Days 5 to 6, respectively. The rate of clearance of i.v.-injected colloidal carbon is also increased for 3 days after lUdR. Thus, lUdR is able to functionally activate either T- or B-cells or macrophages, but only to increase the number of splenic B-cells.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D002244:Carbon; D003208:Concanavalin A / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D007065:Idoxuridine / Q000494:pharmacology*; D007104:Immune Adherence Reaction; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010587:Phagocytosis / Q000187:drug effects; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects*; D013997:Time Factors
D008070:Lipopolysaccharides; D003208:Concanavalin A; D002244:Carbon; D007065:Idoxuridine