Lipid phase transitions in cytoplasmic and outer membranes of Escherichia coli.
The cytoplasmic and outer membranes containing either trans-delta-9-octadecenoate, trans-delta-9-hexadecenoate or cis-delta-9-octadecenoate as predominant unsaturated fatty acid residues in the phospholipids were prepared from a fatty acid auxotroph, Escherichia coli strain K1062. Order-disorder transitions of the phospholipids were revealed in both fractions of the cell envelope by fluorescent probing or wide angle X-ray diffraction. The mid-transition temperatures, Tt, and the range of the transition, delta-T, are similar in the outer and cytoplasmic membrane. Relative to the corresponding extracted lipids, 60-80% of the hydrocarbon chains take part in the transition in the cytoplasmic membrane whereas in the outer membrane only 25-40% of the chains become ordered. The results suggest that in the outer membrane part of the lipids form fluid domains in the form of mono- and/or bilayers.
D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism* / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000032:analysis / Q000378:metabolism*; D005613:Freeze Etching; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D008854:Microscopy, Electron; D010743:Phospholipids / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014961:X-Ray Diffraction
D008055:Lipids; D010743:Phospholipids