Purification and properties of a bacteriophage T5-modified form of Escherichia coli RNA polymerase.
A modified form of Escherichia coli RNA polymerase that contains one of the three T5-specific polypeptides known to interact with the host enzyme was purified from bacteriophage T5-infected cells. The properties of this T5-modified enzyme appeared identical to those of the RNA polymerase derived from uninfected non-colicinogenic cells and to a fully active enzyme isolated from T5-infected ColIb+ cells after the limited in vivo transcription of T5 genes allowed by the plasmid had ceased.
D003090:Coliphages / Q000254:growth & development*; D004267:DNA Viruses / Q000254:growth & development; D012321:DNA-Directed RNA Polymerases / Q000032:analysis / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000032:analysis; D014158:Transcription, Genetic; D014316:Tritium; D014779:Virus Replication
D010455:Peptides; D014316:Tritium; D012321:DNA-Directed RNA Polymerases