Transmission of jaagsiekte (ovine pulmonary adenomatosis) by means of a permanent epithelial cell line established from affected lungs.
An epithelial cell line, designated JS-15,4, has been established in culture from jaagiekte lesions and subcultured in vitro for almost 2 years. It exhibits morphological and other features of transformed cells and has been shown by electron microscopy to consist of type B ovine alveolar epithelial cells. Jaagiekte was successfully transmitted to 3 new-born lambs by the intratracheal injection of cells following immunosuppressive treatment with either anti-thymocyte immunoglobulin alone or combined with anti-macrophage immunoglobulin. Incubation periods as short as 10 weeks were recorded. Evidence was also obtained that natural transmission may result from the inhalation of viable cells.
D000782:Aneuploidy; D000818:Animals; D000831:Animals, Newborn; D000961:Antilymphocyte Serum; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D007074:Immunoglobulin G; D008168:Lung / Q000648:ultrastructure; D008264:Macrophages / Q000276:immunology; D051379:Mice; D011648:Pulmonary Adenomatosis, Ovine / Q000635:transmission*; D011817:Rabbits; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D007074:Immunoglobulin G