[Polarization state of the light source and determination of attenuated total internal reflection spectra in intact microorganisms].
The problems are considered of the interaction of linear polarized light current with undestroyed microorganisms while recording the spectra of attenuated total refraction(ATR) and repeated ATR (RATR). It is shown that proper allowance should be made of dichrosim of absorption bands of microorganisms during quantitative studies. Peculiarities of obtaining RATR spectra of the linearly polarized light source with polarization azinut other than 0 and 90 degrees are considered.
D000190:Actinomyces / Q000032:analysis; D001419:Bacteria / Q000032:analysis*; D002175:Candida / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis; D005658:Fungi / Q000032:analysis*; D013057:Spectrum Analysis / Q000379:methods
Sostoianie poliarizatsii istochnika izlucheniia i osobennosti polucheniia spektra NPVO nerazrushennykh mikroorganizmov