Growth of B-lymphocytes clones in semisolid culture is mitogen dependent.
A substance which was mitogenic for murine B lymphocytes in the presence of 2-mercaptoethanol was isolated from agar. Stimulating activity of this material was stable to proteolysis or protein denaturants but was destroyed by periodate treatment. Agar-derived mitogen stimulation was distinct from that obtained with dextran sulfate or lipopolysaccharide and may define different populations of B lymphocytes.
D000362:Agar; D000818:Animals; D001402:B-Lymphocytes; D002455:Cell Division; D002999:Clone Cells; D051379:Mice; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D008934:Mitogens; D011134:Polysaccharides / Q000494:pharmacology
D008934:Mitogens; D011134:Polysaccharides; D000362:Agar