title: Identification of two populations of immunoglobulin-bearing lymphocytes in man.
abstract: Human peripheral blood lymphocytes bearing easily detectable surface immunoglobulin were quantitated with routine immunofluorescence procedures. The mean value in 17 healthy adults were 18% (range 9 to 30%). When the cells were preincubated at 37 degrees C and washed at the same temperature before staining, the mean value decreased to 9% (range 4.5 to 18%) (p less than 0.001). The mean value increased to 32% (range 15 to 49%) when the cells were prepared and washed at 4 degrees C, and this value was also significantly different from values at room temperature (p less than 0.01). These temperature-related effects were explained by IgG that remained bound to the cell membrane at 4 degrees C, but eluted at 37 degrees C in serum-free medium. The total number of Ig-bearing lymphocytes and T lymphocytes determined by rosette formation with sheep erythrocytes approached 100%. These studies indicate two populations of Ig-bearing lymphocytes in healthy subjects: one with surface-stable Ig determinants, and another that lacks these markers, but has receptors capable of binding IgG.
mesh_terms: D000328:Adult; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology; D004247:DNA / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007104:Immune Adherence Reaction; D007106:Immune Sera; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D007106:Immune Sera; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D014316:Tritium; D004247:DNA; D013936:Thymidine