[Binding of ribosomal RNAs with ribosomal proteins covalently bound to polymer carriers].
Conditions for covalent binding of ribosome proteins from Escherichia coli with insoluble polymers are found. A number of polymer carriers and several methods of protein binding to them were tested, the best one being the fixation of ribosome proteins on cyanogen bromide-activated Sepharose. Binding is studied with fixed on the polymer total 30S and 50S proteins, with individual S7 and S20 proteins, 16S and 23S RNAs in conditions optimal for the reconstruction of 30S subunits in vitro. It is found that at least some of ribosome proteins, being bound with polymer carrier, retain the ability to recognize their specific sites on RNA.
D001665:Binding Sites; D002352:Carrier Proteins / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D007700:Kinetics; D011108:Polymers / Q000494:pharmacology*; D011485:Protein Binding; D012335:RNA, Ribosomal / Q000302:isolation & purification / Q000494:pharmacology*; D012270:Ribosomes / Q000378:metabolism*; D012685:Sepharose / Q000494:pharmacology; D013053:Spectrophotometry; D014461:Ultracentrifugation
D002352:Carrier Proteins; D011108:Polymers; D012335:RNA, Ribosomal; D012685:Sepharose
Sviazyvanie ribosomnykh RNK s belkami ribosom, kovalentno zakreplennymi na polimernykh nositeliakh