Bacteriophage MS-2 in the immune response.
Several aspects of the immune response to bacteriophage MS-2 were studied. In thymectomized, irradiated and bone marrow-reconstituted (TXBM) mice, the response was normal when a high dose of antigen was used. With a 50-fold lower dose of MS-2, the response was impaired, indicating a T-cell involvement in antibody formation. More evidence for the (partial) T-cell dependence of MS-2 was obtained from experiments with anti-thymocyte serum or cyclophosphamide-treated mice. (3H)-thymidine incorporation experiments demonstrated that both B and T cells were active upon in vitro stimulation with MS-2. The dose of MS-2 which was able to induce a normal response in TXBM mice, proved to be optimal for both sensitization and elicitation of a DH. It is concluded that MS-2 is a thymus-dependent antigen which is only thymus independent in high doses.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation / Q000187:drug effects; D000956:Antigens, Viral / Q000008:administration & dosage*; D000961:Antilymphocyte Serum / Q000494:pharmacology; D003090:Coliphages / Q000276:immunology* / Q000302:isolation & purification; D003208:Concanavalin A / Q000494:pharmacology; D003348:Cortisone / Q000008:administration & dosage; D003520:Cyclophosphamide / Q000008:administration & dosage; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000276:immunology
D000906:Antibodies; D000956:Antigens, Viral; D000961:Antilymphocyte Serum; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D037102:Lectins; D008070:Lipopolysaccharides; D003208:Concanavalin A; D003520:Cyclophosphamide; D003348:Cortisone