[Lymphocyte blast transformation in the mouse spleen under the influence of a low-molecular humoral thymus factor in vitro].
The mitogen activity of a low molecular lymphocytosis stimulating substance isolated from the thymus (LSS) has been investigated in cultured mouse CBA spleen cells. LSS caused transformation of lymphocytes to the blast cells capable of mitotic division. The initial signs of transformation were observed after 24 hours of incubation, reaching the maximum in 48 hour old cultures. The stimulating action of LSS held true for about 7 days. The intensity of blastogenesis depended on the dose of LSS. A high concentration LSS inhibited the blastogenic response. B-lymphocytes are not sensitive to LSS. It is concluded that the low molecular humoral factor of the thymus activated specifically the thymus-depended lymphocytes.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D013154:Spleen / Q000187:drug effects*; D013268:Stimulation, Chemical; D013936:Thymidine / Q000378:metabolism; D013949:Thymus Extracts / Q000494:pharmacology*; D013997:Time Factors
D013949:Thymus Extracts; D013936:Thymidine
Blasttransformatsiia limfotsitov selezenki mysheĭ pod vliianiem nizkomolekuliarnogo gumoral'nogo faktora timusa in vitro