Specific cleavage of tRNA by nuclease S1.
Nuclease S1 specifically hydrolizes tRNAs in their anticodon loops, forming new 5' phosphate and 3' OH ends. Some single-stranded regions are not cut by nuclease S1. The strong preference of nuclease S1 for the anticodon region can be used for rapid identification of an anticodon-containing oligonucleotide and subsequent identification of the probable amino acid specificity of tRNA.
D000818:Animals; D000926:Anticodon; D001345:Autoradiography; D001483:Base Sequence; D002642:Chick Embryo; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004926:Escherichia coli; D005347:Fibroblasts; D009841:Oligonucleotides / Q000302:isolation & purification*; D012343:RNA, Transfer / Q000378:metabolism; D012260:Ribonucleases
D000926:Anticodon; D009841:Oligonucleotides; D012343:RNA, Transfer; D012260:Ribonucleases