title: Further characterization of a non-essential mutator gene in Escherichia coli K-12.
abstract: The properties of mutR, a mutator closely linked to thyA, have been further characterized. We have found that the mutator gene is carried on a specialized transducing phage (lambdapcI857 thyA) generated by the excision of lambdacI857 integrated at a secondary attachment site between lysA and thyA. We present three lines of evidence indicating that mutR is a nonessential gene. (i) Deletions of the mutator can be found amoung survivors of heat induction of lambdacI857 when the phage is integrated between lysA and thyA. (ii) Mutations in mutR can be induced with the frameshift mutagen ICR-191. (iii) An amber mutant in mutR has been found. Viable strains could be made by combining the mutator with polB, polA polR, ligts7, and uvrA mutations. The mutator was still able to increase the spontaneous mutation frequency in these genetic backgrounds. When the reversion patterns of a series of well-characterized trpA mutations were analyzed, the results suggested that mutR is more efficient at causing transitions than transversion mutations.
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D003090:Coliphages / Q000254:growth & development; D004260:DNA Repair; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism; D005796:Genes; D005816:Genetic Complementation Test; D008242:Lysogeny; D009153:Mutagens; D009154:Mutation; D013941:Thymine / Q000096:biosynthesis; D014161:Transduction, Genetic; D014364:Tryptophan / Q000096:biosynthesis
chemical_list: D004269:DNA, Bacterial; D009153:Mutagens; D014364:Tryptophan; D013941:Thymine