Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine.
The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic pathway and one enzyme of the arginine biosynthetic pathway were assayed from cells grown under a variety of conditions. Cytidine repressed the synthesis of dihydroorotase (encoded by pyrC), dihydroorotate dehydrogenase (encoded by pyrD), and ornithine transcarbamylase (encoded by argI). Moreover, aspartate transcarbamylase (encoded by pyrB) became further derepressed upon cytidine addition, whereas no change occurred in the levels of the last two enzymes (encoded by pyrE and pyrF) of the pyrimidine pathway. Quantitative nucleotide pool determinations have provided evidence that any individual ribo- or deoxyribonucleoside mono-, di-, or triphosphate of cytosine or uracil is not a repressing metabolite for the pyrimidine biosynthetic enzymes. Other nucleotide derivatives or ratios must be considered.
D000227:Adenine Nucleotides / Q000096:biosynthesis; D000581:Amidohydrolases / Q000378:metabolism; D001120:Arginine / Q000096:biosynthesis / Q000494:pharmacology; D001221:Aspartate Carbamoyltransferase / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D002474:Cell-Free System; D003562:Cytidine / Q000494:pharmacology*; D003597:Cytosine Nucleotides / Q000096:biosynthesis; D004794:Enzyme Repression; D006150:Guanine Nucleotides / Q000096:biosynthesis; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D009963:Orotic Acid / Q000031:analogs & derivatives; D010088:Oxidoreductases / Q000378:metabolism; D010430:Pentosyltransferases / Q000378:metabolism; D011743:Pyrimidines / Q000096:biosynthesis*; D012486:Salmonella typhimurium / Q000201:enzymology* / Q000378:metabolism; D014498:Uracil / Q000494:pharmacology; D014500:Uracil Nucleotides / Q000096:biosynthesis
D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D011743:Pyrimidines; D014500:Uracil Nucleotides; D014498:Uracil; D003562:Cytidine; D009963:Orotic Acid; D001120:Arginine; D010088:Oxidoreductases; D001221:Aspartate Carbamoyltransferase; D009954:Ornithine Carbamoyltransferase; D010430:Pentosyltransferases; D000581:Amidohydrolases; D002262:Carboxy-Lyases