Antibodies to dAT detected by membrane filtration.
Sera from patients with active systemic lupus erythematosus caused the retention of the radioactively labeled synthetic polynucleotide 14C-dAT on cellulose nitrate filters. The amount of 14C-dAT bound correlated with the presence of hypocomplementemia and active renal disease. Sera containing only anti-single-stranded DNA antibodies did not bind 14C-dAT but did bind 125I DNA from E coli that had been prefiltered to remove ssDNA contamination. Inhibition experiments also indicated the presence of antigenic differences between 14C-dAT and prefiltered 125I-E coli DNA. The data suggest that dAT carries one of the major antigenic specificities for antinative DNA antibodies in SLE, and that cellulose nitrate filtration fails to remove a significant proportion of contaminating single-stranded DNA determinants in non-synthetic DNA.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D002250:Carbon Radioisotopes; D004247:DNA / Q000276:immunology; D004269:DNA, Bacterial / Q000276:immunology; D004277:DNA, Single-Stranded / Q000276:immunology; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009393:Nephritis / Q000276:immunology*; D011089:Polydeoxyribonucleotides / Q000276:immunology*; D011849:Radioactive Tracers
D000974:Antibodies, Antinuclear; D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D011089:Polydeoxyribonucleotides; D011849:Radioactive Tracers; D004247:DNA