Simultaneous study of gastric and pancreatic secretion in a preparation perfused ex vivo.
A canine gastroduodenopancreatic block was isolated and perfused ex vivo with homologous blood. This preparation allowed a simultaneous collection of gastric and pancreatic secretion under various conditions of stimulation. To stimulate gastric secretion, pentagastrin, bethanechol chloride or histamine was infused intra-arterially during a six to eight hour period, or both vagi were electrically stimulated. To stimulate pancreatic secretion, secretin was infused by the same method. Secretin infusion inhibited gastric juice volume and acid output by stimulated stomachs and induced an increase in pepsin concentration. Secretin infusion also resulted in a significant increase of flow of pancreatic juice and in both concentration and output of pancreatic secretion of bicarbonate. Arterial perfusion pressure and peripheral vascular resistance of this ex vivo preparation were significantly reduced by secretin. The method described permits the simultaneous study of the secretory function of the stomach and pancreas as well as the evaluation of biochemical changes of perfusion blood and hemodynamic changes in the circuit. Easy accessibility of the vessels and vagus branches also allows direct infusion of drugs and placement of stimulation electrodes.
D000818:Animals; D001774:Blood Chemical Analysis; D001794:Blood Pressure / Q000187:drug effects; D004285:Dogs; D004558:Electric Stimulation; D004573:Electrolytes / Q000378:metabolism; D005260:Female; D005750:Gastric Juice / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010179:Pancreas / Q000502:physiology; D010189:Pancreatic Juice / Q000187:drug effects / Q000378:metabolism*; D010434:Pepsin A / Q000378:metabolism; D010477:Perfusion; D012633:Secretin / Q000008:administration & dosage / Q000494:pharmacology; D013270:Stomach / Q000502:physiology; D014655:Vascular Resistance / Q000187:drug effects
D004573:Electrolytes; D012633:Secretin; D010434:Pepsin A