title: X-linked B-lymphocyte immune defect in CBA/N mice. II. Studies of the mechanisms underlying the immune defect.
abstract: The mechanisms underlying the X-linked thymus-independent (B) lymphocyte functional defect in the CBA/N (CN) mice and their F1 progeny were studied. Immune defective mice were unable to respond to the T-independent antigen 2,4-dinitrophenyl-lysyl-derivative of Ficoll (DNP-lys-Ficoll) but were able to form antibody against the highly cross-reactive hapten (trinitrophenyl) when it was coupled to an erythrocyte carrier. Immune defective CN X DBA/2N (DN) F1 male mice, which do not normally respond to T-independent antigens, were able to respond to both polyribosinic-polyribocytidylic acid and DNP-lys-Ficoll after the administration of CN X DN F1 female spleen cells even if these cells had been depleted of T lymphocytes. In addition, it was shown that the inability of the CN mice and their F1 progeny to respond to T-independent antigens was not due to an intrinsic abnormality of their microenvironment or the suppressive actions of a T lymphocyte. Our data present evidence that the X-linked defect in the CN mice is due to an intrinsic defect in B-lymphocyte development.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage; D001402:B-Lymphocytes / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D003433:Crosses, Genetic; D004140:Dinitrophenols / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives; D008040:Genetic Linkage; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011070:Poly I-C / Q000276:immunology; D011833:Radiation Injuries, Experimental / Q000276:immunology; D012730:Sex Chromosomes; D012756:Sheep / Q000097:blood; D013154:Spleen / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
chemical_list: D000941:Antigens; D004140:Dinitrophenols; D005362:Ficoll; D011070:Poly I-C