Evidence that carrier-specific B cells play a role in cellular collaboration.
Evidence is presented showing that carrier-specific B cells play a significant role in inducing an anti-hapten antibody response to the thymus-dependent antigen, dinitrophenylated monomeric flagellin (DNP-MON), both in vivo and in vitro. Inactivation of carrier-specific B cells by (125I) polymeric flagellin (POL) suicide decreased the anti-DNP IgM response by 40%-50% and reduced the anti-DNP IgG response by 85%-90%. Several lines of evidence established that the suicide procedure eliminated carrier-specific B cells rather than carrier-specific T (helper) cells.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects* / Q000528:radiation effects; D000937:Antigen-Antibody Reactions; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D001426:Bacterial Proteins / Q000494:pharmacology*; D002478:Cells, Cultured; D004140:Dinitrophenols / Q000276:immunology; D005260:Female; D005408:Flagellin / Q000276:immunology / Q000494:pharmacology*; D006241:Haptens; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011830:Radiation Effects; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D001426:Bacterial Proteins; D004140:Dinitrophenols; D006241:Haptens; D005408:Flagellin