Cryopreservation of Trypanosoma cruzi bloodstream forms.
Recovery rates of T. cruzi bloodstream forms subjected to several methods of cryopreservation in liquid nitrogen and at -73 C are reported.  Inoculations of animals with cryopreserved and nonpreserved trypomastigotes revealed that prolonged storage at -196 C apparently did not change the biologic characteristics of different T. cruzi strains.  The reproducibility and consistency of results suggest that "cryobanks" or "reference centers" may be established.
D000818:Animals; D001793:Blood Preservation / Q000379:methods; D005615:Freezing; D006801:Humans; D051379:Mice; D009586:Nitrogen Fixation; D011309:Preservation, Biological / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D014352:Trypanosomiasis / Q000097:blood