Partial purification and properties of an endoribonuclease isolated from human KB cells.
With the use of a precursor to Escherichia coli tRNA-Tyr as a substrate, we have detected and partially purified a novel endoribonuclease from the cytoplasm of human KB tissue culture cells. This activity, which we have called RNase NU, cleaves the tRNA precursor at two sites in that part of the molecule which is not included in the mature tRNA sequence and which is normally degraded in vivo. In keeping with this observation, we have found that, of a variety of substrates tested, only those which are unstable in vivo are attacked by RNase NU. RNase NU can be purified from the 0.2 M NH4Cl wash of ribosomes followed by ammonium sulfate fractionation and DEAE-Sephadex chromatography. RNase NU cleaves RNA to create 3'-phosphate-terminated oligonucleotides. It has a pH optimum near 8.0, requires either a monovalent cation (NH4+ is most efficient) or Ca-2+ for optimal activity, and is inhibited by 0.1 M PO4-3-. In the course of purifying RNase NU we have detected and studied the intracellular distribution of other ribonuclease activities in human KB cells.
D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology; D002277:Carcinoma; D002413:Cations, Divalent; D002414:Cations, Monovalent; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli; D006801:Humans; D007700:Kinetics; D009062:Mouth Neoplasms; D009154:Mutation; D010710:Phosphates / Q000494:pharmacology; D012329:RNA, Bacterial; D012343:RNA, Transfer; D012260:Ribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology; D014443:Tyrosine
D002413:Cations, Divalent; D002414:Cations, Monovalent; D010710:Phosphates; D012329:RNA, Bacterial; D014443:Tyrosine; D012343:RNA, Transfer; D004720:Endonucleases; D012260:Ribonucleases; D002118:Calcium