Pathenogenetic activation of mouse oocytes following avertin anaesthesia.
Avertin anaesthesia induced mouse eggs to become activated parthenogenetically. An increasing incidence of activation was observed when females were anaesthetized 6-5, 9 and 13 h after ovulation, reaching a maximum of 45.8 percent. In a spontaneously ovulating group approximately 12.5 percent of all the eggs ovulated, or 27.3 percent of all the eggs activated evoked a decidual response, and the presence of implanting embryos was confirmed histologically. These findings demonstrate a new and simple method of inducing post-implantation parthenogenetic development in the mouse, and stress the necessity of taking into account the possible consequences of anaesthesia in the early post-ovulatory period.
D000758:Anesthesia; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D010064:Embryo Implantation; D000431:Ethanol; D005260:Female; D005306:Fertilization; D006064:Gonadotropins, Equine / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D008938:Mitosis; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D010060:Ovulation / Q000187:drug effects; D010063:Ovum / Q000502:physiology*; D010312:Parthenogenesis; D013997:Time Factors
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D000431:Ethanol