Passive sensitization of tissue cells. V. The detection of chicken antibodies cytophilic for basophils and eosinophils.
Basophil-cytophilic antibodies were domonstrated in sera of chickens injected with either sheep erythrocytes or avian tuberculin. Normal chicken basophils were passively sensitized in vitro, and two types of cytophilic antibodies were shown by rosette-formation: (a) heat-stable, mercaptoethanol-resistant antibodies, and (b) heat-labile, mercaptoethanol-sensitive antibodies. Serum from one of the chickens injected with sheep erythrocytes also passively sensitized normal, non-basophilic granulocytes which would appear from stained smears to be eosinophils. Cytophilic activity in the serum correlated with the rosette-forming reactions of circulating eosinophils and/or basophils from each of the sensitized donor birds.
D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D001491:Basophils / Q000276:immunology*; D001667:Binding, Competitive; D002645:Chickens; D002850:Chromatography, Gel; D004804:Eosinophils / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D006098:Granulocytes / Q000276:immunology; D006358:Hot Temperature; D007104:Immune Adherence Reaction; D007112:Immunity, Maternally-Acquired; D008623:Mercaptoethanol / Q000494:pharmacology; D014373:Tuberculin
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D014373:Tuberculin; D008623:Mercaptoethanol