Genetical control of B-cell responses. III. Requirement for functional mitogenicity of the antigen in thymus-independent specific responses.
Spleen cells from C3H/HeJ mice fail to respond with polyclonal antibody synthesis to mitogenic concentrations of lipopolysaccharide (LPS) which are optimal for activating spleen cells from a high-responder strain (B10.5M). This unresponsiveness is selective for LPS, since C3H/HeJ cells respond as normals to another B-cell mitogen, purified protein derivative of tuberculin. Spleen cells from low-responder mice also fail to mount a specific anti-NNP plaque-forming cell (PFC) response, when challenged in vitro by NNP-LPS. However, C3H/HeJ cells develop normal responses to another thymus-independent hapten conjugate, DNP-AECM-Ficoll. C3H/HeJ mice fail to mount a specific anti-LPS antibody response, when challenged in vivo with doses of soluble LPS which are fully immunogenic for the high-responder strain. However, C3H/HeJ mice develop normal direct and indirect PFC responses to LPS, when challenged with a thymus-dependent form of the immunogen. These results are interpreted as indicating as absolute requirement for functional mitogenicity of the antigen, in the induction of specific thymus-independent antibody responses.
D000818:Animals; D000907:Antibodies, Bacterial; D000917:Antibody Formation; D000942:Antigens, Bacterial; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D004140:Dinitrophenols; D004926:Escherichia coli / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007125:Immunogenetics; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens; D009596:Nitrophenols; D011135:Polysaccharides, Bacterial; D013045:Species Specificity; D013154:Spleen / Q000166:cytology* / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004140:Dinitrophenols; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D008934:Mitogens; D009596:Nitrophenols; D011135:Polysaccharides, Bacterial