Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci.
A recA-independent recombinational event is described which results in insertion of an entire plasmid genome at a unique site of another plasmid, and coincident excision of a precisely defined DNA segment originally present at the point of the insertion. The resulting recombinant molecules subsequently can undergo site-specific translocation of their component segments or inversion of their original DNA sequence orientation. The events observed entail nonreciprocal exchange of genetic material, and involve a discrete nucleotide sequences that is duplicated in rotationally symmetrical reverse orientation on plasmid DNA (i.e., inverted repeat; palindrome).
D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000378:metabolism; D004586:Electrophoresis; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D005111:Extrachromosomal Inheritance; D005796:Genes; D008854:Microscopy, Electron; D008970:Molecular Weight; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic; D014170:Transformation, Genetic; D014178:Translocation, Genetic
D004269:DNA, Bacterial