The metabolism of saccharin in animals.
Rats given oral doses of [3-14C] saccharin excreted 56-87% of the labeled dose in the urine and 10-40% in the feces during 7 days. Distribution studies showed that the highest levels of 14C were in the kidneys and bladders. The bile was not a significant route of excretion. The presence of labeled CO2 in expired air indicated that saccharin was decarbosylated to a slight degree. DEAE chromatographic separation and isolation of labeled compounds from urine samples showed that more than 99% of the urinary 14C was unchanged saccharin. Up to 1.0% of the 14C was a metabolite identified as 0-sulfamoylbenzoic acid. Comparative metabolic profiles of a dog, rabbit, guinea pig and hamster indicated that there was little difference in the pattern due to animal species or dose level.
D000758:Anesthesia; D000818:Animals; D001565:Benzoates / Q000652:urine; D001646:Bile / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000032:analysis; D005260:Female; D006168:Guinea Pigs; D006868:Hydrolysis; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008297:Male; D011817:Rabbits; D011865:Radioisotope Dilution Technique; D051381:Rats; D012439:Saccharin / Q000378:metabolism* / Q000652:urine; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000378:metabolism; D013997:Time Factors; D001743:Urinary Bladder / Q000378:metabolism
D001565:Benzoates; D002250:Carbon Radioisotopes; D013449:Sulfonamides; D002245:Carbon Dioxide; D012439:Saccharin