Further characterization of antigen-dependent migration inhibition factor in mice.
Antigen-dependent MIF was produced in inbred mice. Lymph node cells from mice sensitized to tubercle bacilli were incubated with small quantities of antigen. The supernatant contained antigen-dependent MIF and its activity when tested on mouse and rabbit spleen was minimal unless PPD was added to the supernatant. The production of the antigen-dependent MIF was T cell-dependent, as shown by the use of anti-theta serum. Its molecular weight was in the range of 50--100,000 and it was concluded that the factor is different from conventional antibodies or antigen-antibody complexes.
D000818:Animals; D000941:Antigens; D008263:Macrophage Migration-Inhibitory Factors / Q000302:isolation & purification*; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology; D014373:Tuberculin
D000941:Antigens; D008263:Macrophage Migration-Inhibitory Factors; D014373:Tuberculin