A method for the selection of pig lymphocytes bearing surface immunoglobulin.
Coupling of IgG anti-immunoglobulin to polystyrene bottles with carbodiimide has been used to bind selectively pig lymphocytes previously treated with anti-immunoglobulin or anti-Ig class-specific fluorescent conjugates. Subsequent recovery of the bound cells produced populations with approximately 80% of cells stained for surface Ig and 90% viability.
D000818:Animals; D002234:Carbodiimides; D002469:Cell Separation; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007074:Immunoglobulin G; D008214:Lymphocytes / Q000276:immunology*; D008722:Methods; D011137:Polystyrenes; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013552:Swine
D002234:Carbodiimides; D007106:Immune Sera; D007074:Immunoglobulin G; D011137:Polystyrenes; D011947:Receptors, Antigen, B-Cell