Advantage of defined tissue slices against minced samples in biochemical in vitro investigations of brain cortex.
A cutting device for the preparation of brain cortex slices with a regular cylindric shape and a defined thickness is described. Determinations of the glucose and the electrolyte metabolism and the reactions of both to substances' additions were made with minced brain and with brain slices of different thichknes. The comparison of the methods and of the results makes evident that experimentation with slices has several advantages.
D000818:Animals; D001929:Brain Edema / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D005110:Extracellular Space; D006019:Glycolysis; D006652:Histological Techniques; D066298:In Vitro Techniques; D010101:Oxygen Consumption; D011817:Rabbits