title: Hyperacute rejection of skin allografts in the mouse. Sensitivity of ingrowing skin grafts to the action of alloantibody and rabbit complement.
abstract: The destructive action of alloantiserum and exogenous complement on ingrowing skin allografts was studied. B6AF1 recipients of a B10.D2 skin graft received a single intravenous injection of B6AF1 anti-B10.D2 serum (antiserum to H-2K.31) together with rabbit complement (RC) within the first 10 days after transplantation. Different models were used: recipients without immunosuppression, recipients treated with antilymphocyte serum, x-irradiation, or enhancing antibody. If the injection was given between day 5 and 10 after grafting, hyperacute rejection occurred in all cases. The rejection seemed to be most violent when the injection was given on days 7 or 8. Injections given on days 1, 2 or 3 after grafting could not induce hyperacute-rejection, but resulted, on the contrary, in a prolongation of graft survival, probably due to immunological enhancement. Injections on day 4 produced patchy necrosis, but the grafts recovered and the residual tissue showed a prolonged survival. The results suggest that the presence of a functioning vascular network is a prerequisite for the occurrence of hyperacute rejection of skin allografts in the mouse.
mesh_terms: D000818:Animals; D003165:Complement System Proteins; D006084:Graft Rejection; D007165:Immunosuppression Therapy; D007518:Isoantibodies; D051379:Mice; D008815:Mice, Inbred Strains; D012867:Skin / Q000098:blood supply; D016038:Skin Transplantation; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D007518:Isoantibodies; D003165:Complement System Proteins