title: Lysosomes and the "toxicity" of Rickettsias. VI. In vivo response of mouse peritoneal phagocytes to L-cell-grown Chlamydia psittaci 6BC strain.
abstract: The L-cell-grown 6BC strain of C. psittaci inoculated intraperitoneally in mice induced an injurious effect on mononuclear phagocytes and their lysosomes; the influx of polymorphonuclear phagocytes (PMN's) increased markedly and the PMN's showed karyorrhexis and lysis. Cytochemical methods failed to detect chlamydial forms in peritoneal fluids from day 1 and up to 6 days after inoculation of mice. Chlamydial infectivity was not detected in either the cell-bound or the cell-free fractions of peritoneal cells from 6 h up to 6 days after inoculation of mice. In contrast, after infection of mice with the egg-grown 6BC strain, readily identifiable multiplication of the parasites occurred from 4 days after inoculation of mice; the lysosomes of macrophages were refractory to egg-grown 6CB particles that were initially ingested and the macrophages were nonspecifically "activated." Some of these "transformed" into large epitheloid cells containing numerous chlamydial inclusions in the cytoplasm. That lysosomes of "professional phagocytes" play a role in the "toxic" effect of virulent chlamydiae in the intact host is discussed.
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001202:Ascitic Fluid / Q000166:cytology; D002642:Chick Embryo; D002689:Chlamydia / Q000472:pathogenicity*; D002690:Chlamydia Infections / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D007739:L Cells; D008247:Lysosomes / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009000:Monocytes / Q000276:immunology / Q000648:ultrastructure; D009504:Neutrophils / Q000648:ultrastructure; D010586:Phagocytes / Q000276:immunology / Q000648:ultrastructure*; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial