Immunoradiometric assay of factor VIII related antigen, with observations in 32 patients with von Willebrand's disease.
A solid phase non-competitive immunoradiometric assay (IRMA) has been developed which allows measurement of factor VIII related antigen (VIIIR:AG) levels in normal plasma as low as 2.5 x 10(-4) U/ml. The assay is based on the extraction of VIIIR:AG from test plasma by means of polystrene tubes coated with a specific unlabelled anti-VIIIR:AG rabbit antiserum and subsequent labelling of the extracted antigen with 125I-labelled anti-VIIIR:AG rabbit IgG.
D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D005169:Factor VIII / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D011863:Radioimmunoassay / Q000379:methods; D013997:Time Factors; D014842:von Willebrand Diseases / Q000235:genetics / Q000276:immunology*
D000941:Antigens; D005169:Factor VIII