Increased immunologic reactivity between human glomerular basement membrane and group A type 12 streptococcal cell membrane after carbohydrase treatment.
Anti-streptococcal cell membrane and anti-human glomerular antisera were evaluated on normal human kidney sections by an indirect immunofluorescent test both before and after absorptions with homologous and heterologous membrane preparations. The observed cross-reactions were potentiated by removal of carbohydrate units from both membrane-types by carbohydrase, a mixture of carbohydrate-cleaving enzymes.
D000818:Animals; D001485:Basement Membrane / Q000276:immunology*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006041:Goats / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007672:Kidney Cortex / Q000276:immunology; D007678:Kidney Glomerulus / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D013291:Streptococcus / Q000276:immunology*
D007106:Immune Sera; D006026:Glycoside Hydrolases