Comparative mitogenic responses of T-cells and B-cells in spleens of mice of varying age.
Mouse spleen cell mitogenic responses to Phytohemagglutinin (PHA), conconavalin A (con A), staphylococcal enterotoxin B (SEB) and lipopolysaccharide (LPS) were studied as a function of age. In CD-1 mice, spleen cell DNA synthesis in response to stimulation by PHA, con A and SEB varied from 18% - 33% of adult values for the first 3 weeks of life. By 6 weeks, the PHA response was 63% of the adult response and achieved adult values by 8 weeks of life. In contrast to these T-cell responses, DNA spleen cell synthesis in response to LPS was approximately that of adults as early as 7 days after birth. When spleen cell mitogenic responses were studied in C57B1/6J mice a similar pattern emerged. C57 spleen cell responses to PHA, con A and SEB varied from 24% - 54% of adult values for the first 3 weeks of life. By 4 weeks, spleen cell responses to PHA and con A were approximately those of adults. Again, C57 spleen cell responses to LPS were equivalent to adult values as early as 7 days after birth.
D000042:Absorption; D000375:Aging; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003208:Concanavalin A; D003601:Cytotoxicity Tests, Immunologic; D004768:Enterotoxins; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D007106:Immune Sera; D037102:Lectins; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D011135:Polysaccharides, Bacterial; D011247:Pregnancy; D011817:Rabbits / Q000276:immunology; D013154:Spleen / Q000166:cytology*; D013210:Staphylococcus / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D004768:Enterotoxins; D007106:Immune Sera; D037102:Lectins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D014316:Tritium; D003208:Concanavalin A; D013936:Thymidine