Freeze-fracture-autoradiography.
A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing. The advantages and limitations of the new method are discussed in terms of monolayer quality, contact, histochemography, resolution, freezing and recrystallisation artefacts.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D003951:Diagnostic Errors; D005260:Female; D005614:Freeze Fracturing; D007053:Ice; D008297:Male; D011893:Rana esculenta
D007053:Ice