The stimulation of immunoglobulin production in murine spleen cells by the pokeweed mitogens.
We have shown that of the five mitogens purified from the salt extracts of pokeweed, only Pa-1, the B cell mitogen, has the ability to stimulate immunoglobulin production in murine B cells in the absence of classical T cells. Furthermore, the T cell mitogens (Pa-2-Pa-5) not only are unable to stimulate DNA or immunoglobulin synthesis by B cells in the absence of T cells, but cannot stimulate immunoglobulin production by B cells even in the presence of T cells. This suggests that Pa-2-Pa-5 do not stimulate adequate helper function in T cells or that if such a helper function is stimulated, an additional stimulatory factor is required for immunoglobulin production, which is not provided for by Pa-2-Pa-5.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D007700:Kinetics; D037102:Lectins; D051379:Mice; D008938:Mitosis; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
D007136:Immunoglobulins; D037102:Lectins; D004247:DNA