Multiple steps in DNA recognition by restriction endonuclease from E. coli K.
The process of DNA recognition by the activated form of the restriction endonuclease from E. coli K involves three enzyme-DNA complexes which can be differentiated experimentally. These are: an initial complex formed at a nonspecific site; a recognition complex involving the host specificity site; and a cleavage complex dependent on the presence of ATP.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D006493:Heparin / Q000494:pharmacology; D007700:Kinetics; D011485:Protein Binding; D012436:S-Adenosylmethionine / Q000378:metabolism; D013329:Structure-Activity Relationship; D014462:Ultrafiltration
D012436:S-Adenosylmethionine; D000255:Adenosine Triphosphate; D006493:Heparin; D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes