Soluble membrane antigen fractions that react with rat enhancing alloantibodies.
Soluble extracts were prepared from cells of Brown Norway (BN) rat spleens by sonication in 2 M KI. Extracts were fractionated by gel filtration and electrophoretic procedures. The fractions specifically inhibited the binding of Lewis alloantibodies to target BN cells in vitro and in vivo. A homogeneous component that retained antigen activity was obtained by preparatory polyacrylamide gel electrophoresis. Lewis rats injected with BN antigen fractions produced histocompatibility alloantisera that would prolong the survival of BN skin grafts applied to Lewis neonates.
D000818:Animals; D000831:Animals, Newborn; D000937:Antigen-Antibody Reactions; D000941:Antigens; D002462:Cell Membrane / Q000276:immunology*; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002860:Chromium Radioisotopes; D003601:Cytotoxicity Tests, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D007106:Immune Sera; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D007518:Isoantibodies; D007525:Isoelectric Focusing; D008214:Lymphocytes / Q000276:immunology*; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D016038:Skin Transplantation; D012995:Solubility; D013154:Spleen / Q000166:cytology; D014020:Tissue Extracts; D014184:Transplantation, Homologous
D000941:Antigens; D002860:Chromium Radioisotopes; D007106:Immune Sera; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D007518:Isoantibodies; D014020:Tissue Extracts