Separation of lymphocyte mitogen from lymphotoxin and experiments on the production of lymphotoxin by lymphoid cells stimulated with the partially purified mitogen: a possible amplification mechanism of cellular immunity and allergy.
A mitogenic factor (MF) from guinea pig lymph node cells (LNC), which was produced by stimulating immune LNC with the specific antigen, ovalbumin, was partially purified by the sequential use of Sephadex G-200 gel filtration. CM-cellulose ion exchange chromatography, DEAE-cellulose ion exchange chromatography, and polyacrylamide disc gel electrophoresis. The product was purified at least 100-fold with regard to protein content. In addition, the purified MF was functionally pure with respect to the absence fo lymphotoxin (LT), another guinea pig lymphokine.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D004305:Dose-Response Relationship, Drug; D004589:Electrophoresis, Disc; D006168:Guinea Pigs; D006967:Hypersensitivity / Q000276:immunology*; D007111:Immunity, Cellular; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology*; D008233:Lymphotoxin-alpha / Q000096:biosynthesis* / Q000276:immunology; D008934:Mitogens / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013997:Time Factors
D000961:Antilymphocyte Serum; D008233:Lymphotoxin-alpha; D008934:Mitogens