title: A method for the separation of lymphocytes and plasma cells from the human palatine tonsil using sedimentation in an isokinetic gradient of Ficoll in tissue culture medium.
abstract: Several methods for the dissociation of human tonsils into cell suspensions were compared. Dissociation of tonsils using 0-25 per cent trypsin gave both the largest number of total cells and the largest number of plasma cells per gram of tonsil. Lymphocytes and plasma cells were separated in a previously described isokinetic gradient of Ficoll in tissue culture medium. In the purest gradient fractions, lymphocytes were 97-2 plus or minus 1-9 per cent of nucleated cells. The purest gradient fractions contained 43-1 plus or minus 5-9 per cent plasma cells. More than 95 per cent of purified lymphocytes and plasma cells excluded Trypan Blue.
mesh_terms: D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D046508:Culture Techniques; D006801:Humans; D007158:Immunologic Techniques; D008214:Lymphocytes; D014066:Palatine Tonsil / Q000166:cytology*; D010950:Plasma Cells; D014343:Trypan Blue; D014357:Trypsin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D014357:Trypsin; D014343:Trypan Blue