Assay for citrovorum factor (NSC-3590) in the presence of methotrexate (NSC-740).
Treatment of certain malignancies with high-dose methotrexate/citrovorum factor rescue has recently been adopted as an effective regimen. A microbiologic assay capable of detecting citrovorum factor in the presence of massive amounts of methotrexate has been developed using a strain of Pediococcus cerevisiae resistant to methotrexate. The assay described in this paper is an inexpensive and rapid method of studying the distribution kinetics of citrovorum factor.
D000328:Adult; D001681:Biological Assay / Q000379:methods; D002648:Child; D003470:Culture Media; D004352:Drug Resistance, Microbial; D006801:Humans; D007934:Leuconostoc / Q000187:drug effects; D002955:Leucovorin / Q000032:analysis* / Q000494:pharmacology; D008727:Methotrexate / Q000494:pharmacology
D003470:Culture Media; D002955:Leucovorin; D008727:Methotrexate