title: alphabeta sequence of F is IS31.
abstract: Previous studies have shown that there is a deoxyribonucleic acid (DNA) segment, of length 1.3 kb and denoted as the alphabeta sequence, which occurs twice on the F plasmid at corrdinates 93.2 to 94.5/OF kb and 13.7 to 15.0F kb. In the present investigation, heteroduplexes were prepared between a phage DNA carrying the insertion sequence IS3 and suitable F-prime DNAs. The hybrids formed show that IS3 is the same as alphabeta. This result plus previous studies support the view that: (i) the insertion sequence IS2 and IS3 occur on F and, in multiple copies, on the main bacterial chromosome of Escherichia coli K-12; and (ii)these IS sequences on the main bacterial chromosomes are hot spots for Hfr formation by reciprocal recombination with the corresponding sequences of F.
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000032:analysis*; D003090:Coliphages; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis*; D004279:DNA, Viral; D004926:Escherichia coli / Q000502:physiology*; D005144:F Factor; D005796:Genes; D009154:Mutation; D009693:Nucleic Acid Hybridization
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral