Isolation of an Escherichia coli mutant deficient in glutathione synthesis.
A mutant of Escherichia coli that contains essentially no detectable glutathione has been isolated. The mutant contains a very low level of the enzyme glutathione synthetase and accumulates lambda-glutamyl cysteine at a concentration approximately equal to the level of glutathione found in its parent. No significant differences in growth were observed between the mutant and its parent. However, the activity of at least one enzyme was found to be affected by the absence of glutathione; the specific activity of the B1 subunit of ribonucleoside diphosphate reductase was greatly reduced. The possibility that the decreased B1 activity is due to a mutation in the structural gene coding for B1 or its regulatory gene could be eliminated. This suggests that one role of glutathione in the cell is to maintain at least this one protein in an active state. We propose the designation gshB for the gene coding for glutathione synthetase.
D002474:Cell-Free System; D004926:Escherichia coli / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D005796:Genes; D005978:Glutathione / Q000096:biosynthesis*; D008769:Methylnitronitrosoguanidine; D009153:Mutagens; D009154:Mutation; D010453:Peptide Synthases / Q000378:metabolism; D012262:Ribonucleoside Diphosphate Reductase / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism; D013879:Thioredoxins / Q000378:metabolism; D014541:Uridine Kinase / Q000378:metabolism; D014543:Uridine Phosphorylase / Q000378:metabolism
D009153:Mutagens; D013438:Sulfhydryl Compounds; D008769:Methylnitronitrosoguanidine; D013879:Thioredoxins; D012262:Ribonucleoside Diphosphate Reductase; D013880:Thioredoxin-Disulfide Reductase; D014543:Uridine Phosphorylase; D014541:Uridine Kinase; D010453:Peptide Synthases; D005978:Glutathione