Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli.
Histone genes have been cloned selectively in Escherichia coli directly from unfractionated sea urchin DNA using labelled mRNA probe. The cloning method used is potentially applicable for isolating any genetic sequence for which a probe is available. The several eukaryotic gene fragments introduced into bacteria by this method collectively contain all or nearly all of the DNA sequences represented in histone mRNA.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002678:Chimera; D004247:DNA / Q000302:isolation & purification*; D004926:Escherichia coli / Q000378:metabolism; D005796:Genes; D005821:Genetic Techniques; D006657:Histones / Q000096:biosynthesis*; D009030:Mosaicism; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis; D012617:Sea Urchins; D014158:Transcription, Genetic
D006657:Histones; D012333:RNA, Messenger; D004247:DNA