title: Studies on energy-linked reactions: genetic analysis of venturicidin-resistant mutants.
abstract: Genetic analysis of venturicidin-resistant mutants has revealed the presence of both nuclear and mitochondrial genes responsible for determining venturicidin sensitivity/resistance in Saccharomyces cerevisiae. Recombination studies show that the mutation with phenotype VENR is situated at mitochondrial locus OL I and is therefore extremely useful of future genetic manipulations as it gives a unique phenotype to this locus distinguishable from the second oligomycin locus OL II. The mutations with phenotype VENR OLYR are linked to oligomycin locus OL I and have been allocated a new mitochondrial locus, namely OL III. Three factor croses involving the venturicidin mutations at loci OL I and OL III have shown them to freely recombine with the other mitochondrial loci R I, R III and OL II. The mitochondrial genetic map is therefore represented as four 'recombinational linkage groups'. A fifth linkage group is also specified for mutants with phenotype VENR TETR, and is probably located on a separate DNA molecule from the four other groups.
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004352:Drug Resistance, Microbial; D004996:Ethidium / Q000494:pharmacology; D008040:Genetic Linkage; D007783:Lactones / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009154:Mutation; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010641:Phenotype; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D000935:Antifungal Agents; D007783:Lactones; D009840:Oligomycins; D004996:Ethidium