[Purification of beta-lactamases by affinity chromatography].
Affinity columns able to purifie beta-lactamases have been prepared either by linking covalently reversible inhibitors or substrates to agarose beds. The enzyme is eluted with a gradient of sodium chloride or released with the substrate. This method is a pertinent one for the purification of these enzymes and for the study of bacteria harbouring more than one beta-lactamase.
D000581:Amidohydrolases / Q000302:isolation & purification*; D000667:Ampicillin / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism; D002510:Cephalosporinase / Q000302:isolation & purification*; D002511:Cephalosporins / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D010405:Penicillinase / Q000302:isolation & purification*; D010406:Penicillins / Q000378:metabolism; D011511:Proteus / Q000201:enzymology*; D012967:Sodium Dodecyl Sulfate
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D010406:Penicillins; D012967:Sodium Dodecyl Sulfate; D000667:Ampicillin; D000581:Amidohydrolases; D002510:Cephalosporinase; D010405:Penicillinase
Purification des beta-lactamases par chromatographie d'affinité