Growth properties and alloantigenic expression of murine lymphoblastoid cell lines.
Murine lymphoblastoid cell lines were evaluated for their expression of Thy-1 and thymus leukemia (TL) differentiation alloantigens.  Two culture conditions were shown to affect this expression.  Cells grown in fetal bovine serum (FBS)-enriched medium expressed up to 15 times the amount of TL as cells grown in horse serum (HS)-enriched medium.  Thy-1 expression was less affected by the type of serum used for culture.  The phase of growth when the cells were harvested, was demonstrated to affect the expression of Thy-1.  The expression of Thy-1.2 for one cell line examined, L-251A, during logarithmic growth was threefold greater than cells collected during either lag or stationary growth.  When culture conditions were standardized a ranking of the amount of Thy-1 and TL expressed by several cell lines was made.  All cell lines, except one, L-1210, expressed Thy-1.  There was a 450-fold difference in the expression of Thy-1 between the cell lines evaluated.  Seven cell lines expressed TL-1,2,3 with a ninefold difference in the amount of expression.  The L-251A cell line was cultured in a 14 liter fermentor for a 26 day period.  During this time TL and Thy-1 expression did not vary significantly, demonstrating that lymphoblastoid cell lines can be cultured on a continuous basis and will continue to express their surface alloantigens.
D000042:Absorption; D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D007106:Immune Sera; D007519:Isoantigens / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D007106:Immune Sera; D007519:Isoantigens