Conjugational behaviour of N plasmids in Escherichia coli K12.
R factors of the N incompatibility group apparently transfer at low frequencies (often 10(-6)-10(-5) per donor cell) in 30 min liquid matings.  However, the level of transfer is greatly increased when donor-recipient mixtures are held on solid medium selective for the recipient only, prior to transconjugant selection.  Increase in transconjugant recovery is still donor-dependent at plating and therefore arises from mating on the plate.  The process limiting N-mediated conjugation in liquid is probably mating pair formation, suggesting that the increase in yield of transconjugants may result from provision of a solid substrate for mating pair formation rather than from the delay in selection per se.  The kinetics of N plasmid transfer on solid medium resemble those of traniments suggest that RP4, the prototype P incompatibility group plasmid, shows a stimulation of transfer on solid medium similar to the N plasmids studied.  We suggest that N, and probably P, plasmids are (like F and the col V's) naturally derepressed for fertility, but this is masked by the imcompetence in liquid matings of donors carrying them.
D003090:Coliphages; D003227:Conjugation, Genetic; D004926:Escherichia coli; D005111:Extrachromosomal Inheritance; D010957:Plasmids; D011815:R Factors; D013997:Time Factors