Cellular immune response in vitro: I. A requirement for time-dependent T-lymphocyte cell division of cytotoxic cells in the allogeneic response.
Immunocompetent lymphoid cells cultured in vitro with allogeneic stimulator cells have been shown to produce T-lymphocyte populations which are specifically cytotoxic in vitro to the stimulatory cells whether normal or malignant. Although the culture requirements as well as the allogeneic requirements are known, the events leading to the production of T-lymphocyte cytotoxic cells is poorly understood. This study examines the role of cell division in the production of allogeneic cytotoxic T-cells in vitro. The elimination of cell division during the first 24 hr of allogeneic culture does not affect the cell-mediated cytotoxic immune response in vitro. Cell division is required, however, from 24 hr through 96 hr in culture and not necessary after 96 hr.
D000818:Animals; D000951:Antigens, Neoplasm; D002455:Cell Division; D003601:Cytotoxicity Tests, Immunologic; D007111:Immunity, Cellular; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000951:Antigens, Neoplasm