Profile analysis of blood proteins with a centrifugal analyzer.
We describe a procedure for routine analysis of a profile composed of eight blood proteins with a GEMSAEC centrifugal analyzer. The end-point technique is used and four standards are included in each run in the immunochemical analysis of the individual proteins. Additional computer programs fit the absorbance readings from the samples to the (nonlinear) standard curve and store the results in a patient's file for a final printout, which also presents the patient's data and reference values. The proteins are analyzed in a given order, which allows common blank runs to be used. Antisera are used in suitably high concentrations, so that the "antigen in excess" problem only appears for analysis of IgA and IgM when sera contain high amounts of the M-components of these two classes. The capacity, accuracy, and precision of the method are satisfactory.
D000937:Antigen-Antibody Reactions; D001322:Autoanalysis; D001798:Blood Proteins / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006242:Haptoglobins / Q000032:analysis; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D008722:Methods; D009961:Orosomucoid / Q000032:analysis; D014168:Transferrin / Q000032:analysis; D000515:alpha 1-Antitrypsin / Q000032:analysis
D001798:Blood Proteins; D006242:Haptoglobins; D007136:Immunoglobulins; D009961:Orosomucoid; D014168:Transferrin; D000515:alpha 1-Antitrypsin