NMR investigation of the effect of selective modifications in the anticodon loop on the conformation of yeast transfer RNA-Phe.
Changes in the 300 MHz proton NMR spectrum of yeast tRNA-Phe induced by the removal of the Y-base from the anticodon loop are reversed when the dye proflavine is incorporated in its place. These observations correlate with our earlier interpretation of the NMR data that removal of the Y-base causes a change in the conformation of the anticodon stem. Such changes in stem conformation may in part be responsible for the differences in the biochemical properties of tRNA-Phe, tRNA-PhePF and tRNA-Phe-Y.
D000926:Anticodon; D001665:Binding Sites; D002852:Chromatography, Ion Exchange; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D010649:Phenylalanine; D011370:Proflavine; D012343:RNA, Transfer / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D000926:Anticodon; D010649:Phenylalanine; D012343:RNA, Transfer; D011370:Proflavine