Assessment of the B-lymphocyte population in agammaglobulinemia.
The population of B lymphocytes was assessed in the blood of 16 patients with agammaglobulinemia using immunofluorescence and EAC1423 reactivity as B-cell markers. Lymphocytes were fractionated on gradients of bovine serum albumin which are capable of separating lymphocytes into B-cell-rich and T-cell-rich populations. There was complete absence of B lymphocytes in the majority of patients with X-linked agammaglobulinemia. Normal or increased numbers of B lymphocytes were found in all patients with common variable agammaglobulinemia.
D000293:Adolescent; D000328:Adult; D000361:Agammaglobulinemia / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D002648:Child; D002675:Child, Preschool; D003165:Complement System Proteins; D003973:Diatrizoate; D004168:Diphtheria Toxoid; D004912:Erythrocytes / Q000276:immunology; D005362:Ficoll; D005455:Fluorescent Antibody Technique; D006041:Goats / Q000276:immunology; D006801:Humans; D007104:Immune Adherence Reaction; D007106:Immune Sera; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012710:Serum Albumin, Bovine; D012730:Sex Chromosomes; D012756:Sheep / Q000276:immunology; D013745:Tetanus Toxoid
D004168:Diphtheria Toxoid; D007106:Immune Sera; D013745:Tetanus Toxoid; D003973:Diatrizoate; D005362:Ficoll; D012710:Serum Albumin, Bovine; D003165:Complement System Proteins