title: Adaptation to tissue culture of the murine leukemia ASL1, a high producer of TL (thymus leukemia) antigen.
abstract: ASL1, a murine thymus derived leukemia, was adapted to tissue culture and its suitability as a source of TL antigens was examined. Tissue culture medium was developed in which the cell grew with a mean doubling time of 12.5  h and 95% viability. The quantity of antigen expressed by the cells was determined by inhibition of lysis of 51Cr labelled leukemic cells meadiated by anti-TL 1,2,3 serum. Significant quantities of the antigens were retained by the cells during exponential growth in vitro.
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells; D000961:Antilymphocyte Serum; D001202:Ascitic Fluid / Q000276:immunology; D046508:Culture Techniques; D003601:Cytotoxicity Tests, Immunologic; D007111:Immunity, Cellular; D007942:Leukemia, Experimental / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000276:immunology*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014022:Tissue Survival
chemical_list: D000961:Antilymphocyte Serum