The selective action of poly A:U on the electrophoretic mobility and surface charge of a subpopulation of B cells.
Electrophoretic mobility (E.P.M.) of lymphoid cells has been determined in three species. In chicks, the E.P.M. of bursa cells was 0.47 plus or minus 0.07 and of thymocytes 0.64 plus or minus 0.10 mum/sec/volt/cm. On the basis of the E.P.M. profile of the Ficoll gradient purified lymphocytes from the rat spleen, two populations of cells present in the proportion of 65 and 35% were identifiable. The former had a mobility of 1.06 plus or minus 0.08 and the latter 1.24 plus or minus 0.09. The E.P.M. of lymphocytes prepared from athymic nude mice was 0.86 plus or minus 0.06 with a Gaussian distribution profile. On incubation of these cells with poly adenylate-poly uridylate (poly A:U) for 30 min at 38 degrees C followed by extensive washings, the E.P.M. of thymocytes from the chick and rat as well as the thymus-derived cells in rat spleen remained essentially unchanged. On the other hand, poly A:U increased the E.P.M. of avian bursa cells and of a subpopulation of B cells in rat spleen and of splenocytes from nude mice. The proportion of cells markedly affected by poly A:U was 70 to 83% of the total B cells in these species.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002060:Bursa of Fabricius / Q000166:cytology; D002645:Chickens; D004586:Electrophoresis; D008564:Membrane Potentials / Q000187:drug effects*; D051379:Mice; D011063:Poly A-U / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000002:abnormalities / Q000166:cytology
D011063:Poly A-U