Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments.
Cytoplasmic RNA, isolated from cells late after infection by adenovirus type 2 and fractionated by hybridization to specific fragments of adenovirus DNA produced by cleavage with the endonuclease R-EcoRI, was used as template for protein synthesis in cell-free mammalian extracts. Each of the R-EcoRI fragments of DNA selects RNA that encodes specific subsets of the viral polypeptides. From the known order of the R-EcoRI fragments, the following partial map is deduced: (III, IIIa, IVa2, V, P-VII, IX), (II, P-VI), 100K, IV-where the relative order of the components enclosed in parentheses has not yet been determined.
D000256:Adenoviridae / Q000032:analysis* / Q000378:metabolism; D002277:Carcinoma; D002460:Cell Line; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005796:Genes; D009062:Mouth Neoplasms; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D013698:Templates, Genetic
D004279:DNA, Viral; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012335:RNA, Ribosomal