Phospholipids bound to acidic nuclear proteins in human B and T lymphocytes.
Chromatin fractions (DNA, histones and non-histone chromosomal proteins NHCP) have been isolated from human peripheral B and T lymphocytes using different methods and analyzed in order to identify their lipid content. While DNA and histone fractions do not reveal the presence of lipids, a 2% of phospholipids is present in the NHCP fraction. The phospholipids associated with NHCP present a constant relative ratio among sphingomyelin, phosphatidyl-choline and phosphatidyl-ethanolamine both in B and T lymphocytes, whichever are the extraction procedures employed. These findings are related to the possible dereprssive role of phospholipids on DNA-dependent RNA synthesis.
D001402:B-Lymphocytes / Q000378:metabolism*; D001665:Binding Sites; D002855:Chromatography, Thin Layer; D004247:DNA / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D008722:Methods; D009698:Nucleoproteins / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011485:Protein Binding; D013601:T-Lymphocytes / Q000378:metabolism*
D006657:Histones; D009698:Nucleoproteins; D010743:Phospholipids; D004247:DNA