Activation of human peripheral blood lymphocytes by concanavalin A dependence of monocytes.
The activation of human peripheral blood lymphocytes or isolated T lymphocytes by concanavalin A (Con A) is hightly potentiated by the presence of autologous, mitomycin C-treated monocytes. The optimal lymphocyte: monocyte ratio within a broad dose range is 1:1 when the incorporation of [14C]thymidine is expressed as total incorporation per culture tube and 1:10 when expressed per lymphocyte. A five-to-ten-fold increase of total DNA synthesis is noted in the presence of 10-90% monocytes. The data may help to explain the wide variations in Con A responsiveness of human peripheral lymphocytes which may be partly related to differences in purification which give rise to cell preparations containing varying amounts of monocytes.
D002458:Cell Fractionation; D002470:Cell Survival; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D008937:Mitomycins / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D013936:Thymidine / Q000378:metabolism
D008937:Mitomycins; D003208:Concanavalin A; D004247:DNA; D013936:Thymidine