A new chemical procedure for 32P-labeling of ribonucleic acids at their 5'-ends after isolation.
A new technique, which utilizes the chemical reaction between [32P]diimidazolidate of orthophosphate and the cetyltrimethylammonium salt of high-molecular-weight RNA in nonaqueous dimethyl formamide, has been developed for the 32P-labeling of RNAs after isolation. The radioactive label of high specific activity is introduced onto a phosphorylated 5'-end of the RNA and renders it suitable for 5'-terminal group analysis. When the labeling reaction was applied to the 70S RNA of avian myeloblastosis virus, a labeled 35S RNA was isolated on sucrose-dimethyl sulfoxide gradients without apparent degradation.
D009189:Avian Myeloblastosis Virus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004121:Dimethyl Sulfoxide; D004926:Escherichia coli / Q000032:analysis; D007553:Isotope Labeling / Q000379:methods*; D008970:Molecular Weight; D009841:Oligonucleotides / Q000302:isolation & purification; D010761:Phosphorus Radioisotopes; D012313:RNA / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification
D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D012329:RNA, Bacterial; D012367:RNA, Viral; D012313:RNA; D004121:Dimethyl Sulfoxide