[Separation and immunological characterization of lymphocytes isolated from human appendix (author's transl)].
The isolation of viable lymphocytes from fresh human appendices is described. Under standardized experimental conditions of cell preparation no selective enrichment of either T- or B-lymphocytes was observed. The mean percentage of T-lymphocytes as judged by spontaneous rosette formation found in 34 appendices examined was 50.0%. After overnight incubation surface-Ig was detected by immunoradioautography on 46.8% of the recovered lymphocytes from 10 appendices. For the detection of surface-Ig specifically purified antibodies to heavy chains mu, gamma and alpha were used. Of the 46.8% surface-Ig positive lymphocytes the class distribution was as follows: IgM 17.7%, IgG 24.4% and IgA 4.7%. The isolated lymphocytes showed a variable mitotic response to PHA (stimulation index ranging from 3.3 to 200.9) as well as to allogeneic lymphocytes in a one-way mixed lymphocyte culture. So far, a distinct correlation could not be found between the histological degree of inflammation and the class or mitotic response of appendix lymphocytes.
D000293:Adolescent; D000328:Adult; D001064:Appendicitis; D001065:Appendix / Q000276:immunology*; D001345:Autoradiography; D001402:B-Lymphocytes / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D066298:In Vitro Techniques; D007223:Infant; D007457:Iodine Radioisotopes; D037102:Lectins / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008938:Mitosis / Q000187:drug effects; D013499:Surface Properties; D013601:T-Lymphocytes / Q000276:immunology
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007457:Iodine Radioisotopes; D037102:Lectins
Isolierung und immunologische Charakterisierung von Lymphocyten aus humanen Appendices