Simultaneous ribosomal resistance to trichodermin and anisomycin in Saccharomyces cerevisiae mutants.
A spontaneous mutant of Saccharomyces cerevisiae resistant to trichodermin has been isolated. It displays cross resistance both in vivo and in vitro to a number of sesquiterpene antibiotics (fusarenon X, trichothecin and verrucarin A) and to the chemically unrelated antibiotic anisomycin. The mutation conferring resistance to anisomycin and trichodermin is expressed in the 60-S subunit of the yeast 80-S ribosome. Mutant ribosomes bind [-14C]trichodermin much less efficiently than wild type ribosomes, suggesting that resistance may be due, at least in part, to this property. However, both types of ribosomes bind [-3H] anisomycin equally. These results suggest that anisomycin and trichodermin have different binding sites on the 60-S subunit of eukaryotic ribosomes, even though previous results have shown that both antibiotics bind to mutually exclusive sites.
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001665:Binding Sites; D002455:Cell Division / Q000187:drug effects; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000096:biosynthesis; D009154:Mutation; D014176:Protein Biosynthesis / Q000187:drug effects; D011955:Receptors, Drug; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D005656:Fungal Proteins; D011955:Receptors, Drug; D013729:Terpenes