Platelet indirect radioactive Coombs test. Its utilization for PLA1 grouping.
A platelet indirect radioactive Coombs test (PIRC) has been described. The technique for purification and labelling the antiglobulin has been precised. This test allowed the typing of platelets in the PLA system by using an absorbed serum from a mother of a thrombocytopenic child. Six other families of neonatal thrombocytopenias were tested. In three of them, the mothers were found PLA1 negative (PLA2, PLA2). Among a panel of 93 platelets, two (0.022) were found PLA1, negative. This PIRC test has many advantages compared to other tests such as platelet complement fixation, assay for blocking antibodies or antiglobulin consumption: it gives objective and quantitative results and is highly reproducible; anticomplementary serum may be tested.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D001792:Blood Platelets / Q000276:immunology*; D002452:Cell Count; D003298:Coombs Test / Q000379:methods*; D005260:Female; D006041:Goats / Q000276:immunology; D006680:HLA Antigens; D006801:Humans; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D007518:Isoantibodies; D008297:Male; D010375:Pedigree; D010641:Phenotype; D013921:Thrombocytopenia / Q000235:genetics / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D006680:HLA Antigens; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D007518:Isoantibodies