title: Comparison of four methods for determining nitrate utilization by cryptococci.
abstract: This study evaluated the following methods for determining nitrate utilization: Wickerham broth, a special nitrate broth, Delft plate, and nitrate strip. With 236 isolates of cryptococci as test organisms, the special nitrate broth method gave 99% correct results and the Wickerham broth method gave 98%. The nitrate strip and Delft plate methods gave correct results in 94 and 86% of tests, respectively. The special nitrate broth method is judged superior because it provides accurate results within 48 h, compared to 14 days with the Wickerham broth method.
mesh_terms: D003454:Cryptococcus / Q000378:metabolism*; D003455:Cryptococcus neoformans / Q000378:metabolism; D003470:Culture Media; D005069:Evaluation Studies as Topic; D008722:Methods; D009566:Nitrates / Q000378:metabolism*; D011934:Reagent Strips
chemical_list: D003470:Culture Media; D009566:Nitrates; D011934:Reagent Strips