Studies on column fractionation of immune cells. VI. Separation of thymus-derived lymphocytes by means of protein beads coated with antigen-antibody complexes.
Calf serum beads coated with antigen-antibody complexes were used as cellular immuno-adsorbents to separate mouse T-lymphocytes with a purity of more than 90%. The beads coated by means of glutaraldehyde could be used at least three times without loss of cell-binding capacity.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000917:Antibody Formation; D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D002417:Cattle; D002458:Cell Fractionation; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003165:Complement System Proteins