Immobilized L-asparginase embedded in fibrin polymer.
Immobilized asparaginase was prepared by embedding asparaginase (which is effective for remission in children with leukemia) into fibrin polymer formed by fibrinogen-fibrin conversion in the presence of thrombin. The immobilized asparaginase film did not dissolve in 6 mol/1 urea, suggesting that blood coagulation factor XIII participates in the cross-linking between fibrins and between fibrin and asparaginase.
D000818:Animals; D001215:Asparaginase / Q000378:metabolism*; D002417:Cattle; D004926:Escherichia coli / Q000201:enzymology; D005176:Factor XIII / Q000378:metabolism; D005337:Fibrin; D046911:Macromolecular Substances; D008722:Methods; D011485:Protein Binding; D012995:Solubility; D013917:Thrombin
D046911:Macromolecular Substances; D005337:Fibrin; D005176:Factor XIII; D013917:Thrombin; D001215:Asparaginase