Lysosomal acid phosphatase: difference between normal and chronic lymphocytic leukaemia T and B lymphocytes.
Lysosomal acid phosphatase was assayed in homogenates of isolated normal and B cell type chronic lymphocytic leukaemia (B-CLL) T and B lymphocytes by biochemical means. Unlike the results of cytochemical studies reported in the literature enzyme activity was considerably higher in normal B lymphocytes than in corresponding T cells. This finding offers the possibility to use acid phosphatase as a marker for normal B lymphocytes. The diminution of acid phosphatase in unseparated B-CLL lymphocytes depends predominantly upon a loss of enzyme activity in the B cell fraction indicating an intrinsic abnormality of these neoplastic lymphocytes.
D000135:Acid Phosphatase / Q000032:analysis*; D001402:B-Lymphocytes / Q000201:enzymology*; D002474:Cell-Free System; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008722:Methods; D013601:T-Lymphocytes / Q000201:enzymology*
D000135:Acid Phosphatase