title: The fate of injected human IgG in the mouse liver: Uptake, immunological inactivation, and lysosomal reactions.
abstract: 1. Human IgG is stored in the form of remarkable droplets in the cytoplasm of mouse hepatocytes following intravenous injection. 2. The earliest protein uptake occurs within 1 minute after injection and droplets can be found up to 32 hours thereafter; 64 hours p.i. the foreign protein is no longer visible in hepatocytes. 3. Electron microscopy reveals that the uptake occurs by a process of "macropinocytosis". The resulting protein droplets fuse with primary lysosomes and are transformed into phagolysosomes. 4. At the same time as the phagolysosomes are formed the IgG-droplets loose their immunological activity--as early as 2 hours after injection.
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D006801:Humans; D007074:Immunoglobulin G / Q000008:administration & dosage*; D007275:Injections, Intravenous; D008099:Liver / Q000187:drug effects* / Q000276:immunology; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010586:Phagocytes; D010873:Pinocytosis; D013997:Time Factors
chemical_list: D007074:Immunoglobulin G; D000135:Acid Phosphatase