Effect of stimulation with bacterial lipopolysaccharide on the surface-charge of mouse B-lymphocytes.
Nude spleen (B) cells were cultivated in vitro in a serum-free medium, without or with lipopolysaccharide (LPS) and their surface-charge was measured by cell electrophoresis. In the absence of LPS, surface-charge did not vary significantly with time, whereas, in the presence of LPS a biphasic modification was observed. Thus, after 4 hours of culture with LPS, surface-charge was diminished by 14%. Subsequently, when DNA synthesis and blast formation were activated, it increased by up to 19-23% over control value, whether LPS was present continuously in the medium or had been eliminated by washing after 4 hours of incubation.
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002462:Cell Membrane; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004586:Electrophoresis; D008070:Lipopolysaccharides / Q000378:metabolism*; D008564:Membrane Potentials; D051379:Mice; D008819:Mice, Nude; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism*
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D004247:DNA