[Use of I125 labelled poly(I). poly(C) for determination of antibodies to double-stranded RNA by immune complex absorption to nitrocellulose filters].
125I-labeled double-stranded polyribonucleotide complex was used for detection of antibodies to double-stranded RNA in sera from people and immunized animals by the method of immune complex adsorption by the nitrocellulose filters. The technique is simple and sensitive. Antibodies to double-stranded RNA WERE detected in sera from patients with different diseases and from normal individuals. Systemic lupus erythematosus sera contain as a rule higher amounts of antibodies to double-stranded RNA. Often these antibodies were measured together with those directed towards native and denatured DNA. Anti-double-stranded RNA antibodies from sera of immunised animals and patients with systemic lupus erythematosus are highly specific.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D003095:Collagen Diseases / Q000276:immunology*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D011070:Poly I-C / Q000276:immunology*; D012313:RNA / Q000276:immunology*; D012335:RNA, Ribosomal; D011817:Rabbits; D012216:Rheumatic Diseases / Q000276:immunology; D012595:Scleroderma, Systemic / Q000276:immunology
D000906:Antibodies; D012335:RNA, Ribosomal; D012313:RNA; D011070:Poly I-C
Ispol'zovanie mechennoĭ 125I poli(I). poli(C) dlia epredeleniia antitel k dvuspiral'noĭ RNK metodom adsorbtsii immunogo kompleksa na nitrotselliuloznykh fil'trakh