Purification of kallikrein from cat submaxillary gland.
Cat submaxillary kallikrein was purified from the water extract of the gland by 50-75% acetone fractionation, DEAE-Sephadex A-50 chromatography, Sephadex G-75 gel filtration and Ampholine isoelectric focusing. The final preparation gave the activities of 1260 KU/mg in vasodilator assay, 24.5 and 11.6 units/mg on BAEE and TAME esterolytic assay, respectively. Trasylol gave only weak inhibitory effect on this kallikrein. The isoelectric points of the kallikrein were in the range pH 4.2-5.0, and this kallikrein seemed to consist of several multiple forms with different pI values, like some other glandular kallikreins. It gave a single protein band in disc electrophoresis, while it migrated in retard and formed a broad one. This may be ascribable to the rich carbohydrate content in the kallikrein.
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D002415:Cats; D007610:Kallikreins / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D013363:Submandibular Gland / Q000201:enzymology*
D007611:Aprotinin; D007610:Kallikreins