Transfer of E1 and V colicinogenic factors to Shigella flexneri.
From Escherichia coli strains, ColE1-ML and ColV-K94 factors were transferred to a virulent Shigella flexneri 2a strain at 1-2% frequency. S. flexneri ColV+ and ColE1+V+ produced Col- segregants. The ColE1 factor was stably incorporated into S. flexneri. The ColV+ culture lost its virulence to the guinea pig eye.
D000818:Animals; D003087:Colicins / Q000096:biosynthesis*; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D004926:Escherichia coli / Q000378:metabolism*; D005111:Extrachromosomal Inheritance; D006168:Guinea Pigs; D007637:Keratoconjunctivitis / Q000382:microbiology; D012763:Shigella flexneri / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
D003087:Colicins