Effect of concanavalin A and phytohemagglutinin on the modification of immunogenicity of canine kidney allografts.
Mongrel dog kidneys were allografted to unrelated nephrectomized recipients which were then treated with subimmunosuppressive doses of azathioprine (2.5 mg/kg/day). Dog kidneys treated in vitro with perfusates containing concanavalin A (Con A) or phytohemagglutinin-P (PHA) survived as long as 60 days (mean 39.8 +/- 4.3) after transplantation, whereas normal kidneys survived less than 16 days. The optimal prolongation was achieved by perfusing the kidneys with 500 ml Ringer's lactate containing 25 mg/L Con A, 25 4 degrees C. Lesser effects were achieved with higher or lower concentrations of Con A, or with perfusions carried out at 25 degrees C. Most evidence suggests that Con A and PHA bind to cell surfaces and interfere with the perception of the graft antigens by the host.
D000818:Animals; D001379:Azathioprine / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology*; D004285:Dogs; D006084:Graft Rejection / Q000187:drug effects*; D016030:Kidney Transplantation; D037102:Lectins / Q000494:pharmacology*; D013997:Time Factors; D014184:Transplantation, Homologous
D037102:Lectins; D003208:Concanavalin A; D001379:Azathioprine