Resolution of the DNA strands of the specialized transducing bacteriophage lambda-h80C 1-857 dargF.
The DNA strands of lambdoid phages with deletions or substitutions of the guanine plus cytosine-rich region in the left arm are not resolvable by complexing with poly UG followed by centrifugation in CsCl. This work describes a completely general procedure for the strand resolution of these phages by hybridization with fragments of separated strands of the parent phage. In particular, resolution of the DNA strands of the specialized transducing phage lambda-h80C1-857dargF is described, and evidence is presented which indicates that argF is transcribed from the r strand.
D001120:Arginine / Q000096:biosynthesis; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D003090:Coliphages / Q000032:analysis*; D004277:DNA, Single-Stranded; D004279:DNA, Viral / Q000302:isolation & purification*; D004926:Escherichia coli / Q000378:metabolism; D005796:Genes; D006882:Hydroxyapatites; D009154:Mutation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D011119:Polynucleotides / Q000378:metabolism; D012333:RNA, Messenger; D012367:RNA, Viral; D014158:Transcription, Genetic; D014161:Transduction, Genetic
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D006882:Hydroxyapatites; D011119:Polynucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D001120:Arginine