Erythrocyte receptors and thymus-associated antigens on human thymocytes, mitogen-induced blasts, and acute leukemia blasts.
Human thymus cells and blasts from some patients with acute lymphoblastic leukemia (ALL) express similar cell surface properties. This suggests that this type of ALL originates in the thymus or alternatively that these properties of ALL blasts reflect changes occurring during blastogenesis. To test these possibilities, we determined whether mitogen-thymus antigens found on blasts from E+ALL  and on normal human thymocytes. E-rosettes of blood T-lymphocytes dissociated at 37 degrees; in contrast, rosettes formed by E+ALL blasts, human thymocytes, and pokeweed mitogen-stimulated blasts were stable at this temperature. Blasts that formed stable rosettes did not have cytoplasmic Ig, indicating that they were T-lymphoblasts. By immunofluorescence and a radiolabeled antibody assay we demonstrated a thymus antigen(s) that was present on the membrane of E+ALL blasts and on normal thymocytes, but not on normal blood T-lymphocytes [TL-like antigen(s)]. This antigen was not expressed on blasts induced by mitogens. The finding that that mitogen-induced blasts form temperature-stable rosettes, but lack TL-like antigen(s), indicates that this antigen is not required for the expression of E-receptors stable at 37 degrees. The results support the concept that E+ALL results from the malignant transformation of thymus cells.
D000941:Antigens; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology; D002648:Child; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007104:Immune Adherence Reaction; D007106:Immune Sera; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008213:Lymphocyte Activation; D008934:Mitogens / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D007106:Immune Sera; D008934:Mitogens