A technic for the collection of peritoneal cells from laboratory animals.
A simple method was devised for the collection of peritoneal cells from rats, Chinese hamsters, and mice using a special glass pipette. Cell collection was performed by lavage on the intact unanesthetized animal within 30-60 sec, and it yielded 5-20 million cells per rat and 0.3-2.5 million cells per mouse or Chinese hamster. The procedure can be repeated on different occasions in the same animal without apparent adverse effects.
D000818:Animals; D000830:Animals, Laboratory; D001202:Ascitic Fluid / Q000166:cytology*; D006224:Cricetinae; D003584:Cytological Techniques / Q000295:instrumentation; D051379:Mice; D051381:Rats