Effect of cytostatic drugs on nucleic acid synthesis in mouse thymocytes and chick embryo cells.
Cell suspensions of either mouse thymocytes or chick embryo cells were incubated with labelled precursors after preincubation with different cytostatic drugs. The total time of in vitro incubation was four hours. The ratio between the amount of incorporated labelled precursors and the total amount of DNA was determined and used to measure the cytostatic effect. All drugs used caused a marked depression of this ratio. The cytostatic drugs had no effect on the phosphorylation of thymidine.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo / Q000166:cytology*; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D003609:Dactinomycin / Q000494:pharmacology; D003839:Deoxyadenosines / Q000378:metabolism; D003857:Deoxyuridine / Q000378:metabolism; D005472:Fluorouracil / Q000494:pharmacology; D008558:Melphalan / Q000494:pharmacology; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology*; D014228:Triaziquone / Q000494:pharmacology; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology
D000970:Antineoplastic Agents; D003839:Deoxyadenosines; D003561:Cytarabine; D014316:Tritium; D003609:Dactinomycin; D014747:Vinblastine; D004247:DNA; D014228:Triaziquone; D008558:Melphalan; D005472:Fluorouracil; D013936:Thymidine; D003857:Deoxyuridine; D014529:Uridine; D008727:Methotrexate