Differential cytotoxicity of tumorigenic and nontumorigenic strain-2 guinea pig cells as mediated by syngeneic phytohemagglutinin-stimulated peritoneal exudate cells.
Phytohemagglutinin (PHA)-stimulated peritoneal exudate (PE) cells from strain-2 guinea pigs were more cytotoxic in culture to syngeneic tumorigenic cells than to syngeneic nontumorigenic cells. Cytotoxicity was measured by the release of 3H-thymidine from prelabeled target cells. Tumor-producing guinea pig fetal cells transformed in culture by chemical carcinogen released up to 70 percent of their label in the presence of PHA-stimulated PE cells. Non-tumor-producing cells, regardless of their morphologic characteristics, were less affected by PHA-stimulated PE cells. Nontumorigenic cultures included untreated early passage and long-term cultures previously treated with a carcinogenic or noncarcinogenic chemical. Differential cytotoxicity to tumorigenic cultures were best distinguished from nontumorigenic cultures when 0.5-1 times 10-6 PE cells and 50-100 mug PHA were incubated with 1 times 10-4 3H-thymidine-labeled target cells for 48 hours.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D001500:BCG Vaccine; D002273:Carcinogens; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003601:Cytotoxicity Tests, Immunologic; D006168:Guinea Pigs / Q000276:immunology; D006968:Hypersensitivity, Delayed; D037102:Lectins / Q000276:immunology*; D009163:Mycobacterium bovis / Q000276:immunology; D009374:Neoplasms, Experimental / Q000209:etiology / Q000276:immunology*; D014374:Tuberculin Test
D001500:BCG Vaccine; D002273:Carcinogens; D037102:Lectins