title: Studies on the mechanism of stimulation of the humoral response of murine spleen cultures by culture fluids from human monocytes.
abstract: It has been demonstrated that media derived from cultures of human monocytes can stimulate the IgM and IgG anti-sheep erythrocyte response of normal or T cell-deficient murine splenocytes in vitro. This culture fluid also causes a slight stimulation in the rate of B cell division and in the number of antibody-secreting cells occurring in the absence of added antigen. The active factors, which are released from the monocytes during the first 2 days of culture, appear to affect the murine B cells throughout the culture period.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D002469:Cell Separation; D002478:Cells, Cultured; D003208:Concanavalin A; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006801:Humans; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000276:immunology*; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014316:Tritium; D003208:Concanavalin A; D013936:Thymidine