Blast transformation of rabbit B-derived lymphocytes by a mitogen extracted from Nocardia.
Nocardia water soluble mitogen (NWSM) is known to stimulate mouse and rabbit lymphoid cells and to act selectively on murine B-derived lymphocytes. In this paper, evidence is presented that NWSM is also a mitogen for rabbit bursal equivalent cells and does not bring about blast transformation of thymus-derived rabbit cells. Pretreatment of rabbit spleen lymphocytes with antibody directed against rabbit thymus lymphocyte antigen (anti RTLA serum) and with complement did not affect the strong increase of thymidine incorporation which follows stimulation with NWSM. The mitogen-induced polyclonal activation of antibody-forming cells and resulted in the presence of 30% of cells with the ultrastructural characteristics of plasmocytes. These observations led to the conclusion that NWSM is a mitogen for a B-derived lymphocyte of the rabbit.
D000818:Animals; D000921:Antibody-Producing Cells; D001402:B-Lymphocytes / Q000276:immunology*; D002452:Cell Count; D002478:Cells, Cultured; D002999:Clone Cells; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006736:Horses / Q000276:immunology; D007106:Immune Sera; D037102:Lectins; D008070:Lipopolysaccharides; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000648:ultrastructure; D008854:Microscopy, Electron; D008934:Mitogens; D009596:Nitrophenols / Q000276:immunology; D009615:Nocardia / Q000276:immunology*; D011135:Polysaccharides, Bacterial; D011817:Rabbits; D012477:Salmonella enteritidis / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology; D014316:Tritium
D007106:Immune Sera; D037102:Lectins; D008070:Lipopolysaccharides; D008934:Mitogens; D009596:Nitrophenols; D011135:Polysaccharides, Bacterial; D014316:Tritium; D013936:Thymidine