title: Effect of concanavalin A and phytohemagglutinin on the modification of immunogenicity of canine kidney allografts.
abstract: Mongrel dog kidneys were allografted to unrelated nephrectomized recipients which were then treated with subimmunosuppressive doses of azathioprine (2.5 mg/kg/day). Dog kidneys treated in vitro with perfusates containing concanavalin A (Con A) or phytohemagglutinin-P (PHA) survived as long as 60 days (mean 39.8 +/- 4.3) after transplantation, whereas normal kidneys survived less than 16 days. The optimal prolongation was achieved by perfusing the kidneys with 500 ml Ringer's lactate containing 25 mg/L Con A, 25 4 degrees C. Lesser effects were achieved with higher or lower concentrations of Con A, or with perfusions carried out at 25 degrees C. Most evidence suggests that Con A and PHA bind to cell surfaces and interfere with the perception of the graft antigens by the host.
mesh_terms: D000818:Animals; D001379:Azathioprine / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology*; D004285:Dogs; D006084:Graft Rejection / Q000187:drug effects*; D016030:Kidney Transplantation; D037102:Lectins / Q000494:pharmacology*; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D037102:Lectins; D003208:Concanavalin A; D001379:Azathioprine