Proteins of the newt oocyte nucleus: analysis of the nonhistone proteins from lampbrush chromosomes, nucleoli and nuclear sap.
An electrophoretic analysis has been carried out on the total protein of lampbrush chromosomes, nucleoli and nuclear sap, obtained from newt oocyte nuclei. In each case, distinctive and heterogeneous banding patterns are observed. The absence of detectable quantities of histones in occyte chromatin is noted. In the case of the lampbrush chromosome preparation, it is concluded that all protein species are derived from the ribonucleoprotein matrix of the lateral loops.
D000818:Animals; D002458:Cell Fractionation; D002466:Cell Nucleolus / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis*; D002843:Chromatin / Q000032:analysis; D002875:Chromosomes / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006657:Histones / Q000032:analysis; D008858:Microscopy, Phase-Contrast; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis*; D009865:Oocytes / Q000032:analysis*; D010063:Ovum / Q000032:analysis*; D012995:Solubility; D014319:Triturus / Q000502:physiology*
D002843:Chromatin; D006657:Histones; D009698:Nucleoproteins