Effect of anti-donor serum (ADS) and donor cells on renal allograft survival in DL-A tissue-typed littermate beagles.
The influence of passive and active immunization on kidney allograft survival was tested in littermate beagles differing in one and two DL-A haplotypes. Immunization by treatment of recipients with hyperimmune antidonor serum (ADS) prolonged graft survival only in a few donor-recipient combinations differing in one DL-A haplotype. All ADS batches that were tested in MLC reaction did block this reaction, but no correlation with its in vivo activities was found. Hyperimmunization of recipients with donor lymphoid cells before grafting caused a second-set rejection, whereas one injection with donor blood did so only in 14%, with antibody production and accelerated rejection. A short course of horse antidog lymphocyte serum (HADLS) prolonged kidney graft survival. The addition of intravenously given donor bone-marrow cells to this protocol did not lead to a further increase in graft survival.
D000818:Animals; D000961:Antilymphocyte Serum; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D003601:Cytotoxicity Tests, Immunologic; D004285:Dogs / Q000276:immunology*; D006388:Hemagglutinins / Q000032:analysis; D006648:Histocompatibility; D007112:Immunity, Maternally-Acquired; D016030:Kidney Transplantation; D008214:Lymphocytes / Q000276:immunology*; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D000961:Antilymphocyte Serum; D006388:Hemagglutinins