[Utilization of luminescent monospecific sera against human immunoglobulins G, A and M for diagnostic purposes].
A method for obtaining luminescent sera, consisting in isolation of pure antigens G, A, M from human gamma-globulin and blood serum from patients with myeloma disease is suggested. A native antiserum was obtained by immunization of rabbits with water-insoluble polycondensate of antigens "sewn" with glutaric aldehyde. Adsorption of antisera as well as specific antigens was carried out with antigen- and antibodyimmunosorbent, the latter being obtained with the help of both glutaric aldehyde and sefarose 4B treated with cyanogen bromide. The sera had a specific titre in the precipitation reaction against their own antigens 1:32 and were highly specific. A globulin fraction was obtained by sedimentation with polyethylene-glycol. Marking of the specific protein with fluoresceine isothiocyanate was carried out using the dialysis method with subsequent purification on sefadex and DEAE-cellulose. The application of the abovementioned sera made it possible to ascertain the character of distribution of deposits of immunoglobulins in glomeruli in systemic lupus erythematodes, glomerulonephritis and in the cells of the synovial fluid sediment in rhematoid arthritis.
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000175:diagnosis; D005455:Fluorescent Antibody Technique / Q000379:methods; D005921:Glomerulonephritis / Q000175:diagnosis; D006801:Humans; D007114:Immunization; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008163:Luminescent Measurements; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis; D012698:Serologic Tests / Q000379:methods*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Ispol'zovanie liuminestsiruiushchikh monospetsificheskikh syvorotok protiv immunoglobulinov G, A, M cheloveka dlia diagnosticheskikh tseleĭ