Synthesis of pppGpN type dinucleotide derivatives: the 5' end sequence of some RNAs.
A rapid and simple synthesis of pppGpN type /N equals C, U or A/ diribonucleotide derivatives is described by coupling guanosine 2', 3'-cyclic phosphate 5'-triphosphate with the appropriate ribonucleoside in the presence of ribonuclease T-1.
D000469:Alkaline Phosphatase; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000201:enzymology; D006150:Guanine Nucleotides; D008722:Methods; D009841:Oligonucleotides / Q000096:biosynthesis*; D009905:Optical Rotatory Dispersion; D012313:RNA; D012260:Ribonucleases; D013056:Spectrophotometry, Ultraviolet
D006150:Guanine Nucleotides; D009841:Oligonucleotides; D012313:RNA; D012260:Ribonucleases; D000469:Alkaline Phosphatase