Problems encountered in the preparation of migration inhibitory factor (MIF) and mitogenic factor (MIT) from phytomitogen-stimulated human peripheral lymphocytes and in the preparation of MIF from human lymphoid cell lines (LCL).
D000409:Alanine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D002460:Cell Line; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D005947:Glucose / Q000032:analysis; D005973:Glutamine / Q000032:analysis; D006386:Hemagglutination Tests; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis / Q000302:isolation & purification*; D008934:Mitogens
D000596:Amino Acids; D037102:Lectins; D008263:Macrophage Migration-Inhibitory Factors; D008934:Mitogens; D005973:Glutamine; D003208:Concanavalin A; D005947:Glucose; D000409:Alanine