The "hidden ligand" of the galactose-binding protein.
Following tryptophan fluorescence of the galactose-binding during dissociation of the ligand it has been found that glucose dissociates with a half life of less than 5 s. Similarly, fast dissociation was also observed by following release of radioactively labelled glucose from Sepharose-coupled galactose-binding protein upon dilution. Accordingly, a previous claim that the galactose-binding protein contains glucose as a non-dissociable "hidden ligand" [G. Richarme and A. Kepes (1974) Eur. J. Biochem. 45, 127-133] has to be reinterpreted
D002352:Carrier Proteins; D003956:Dialysis; D004926:Escherichia coli; D005690:Galactose; D005947:Glucose; D006207:Half-Life; D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D012685:Sepharose; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan
D002352:Carrier Proteins; D008024:Ligands; D014364:Tryptophan; D012685:Sepharose; D005947:Glucose; D005690:Galactose