Discussion paper: impairment of B-lymphocyte functions in concanavalin A-treated friend virus infected mice.
Friend leukemia virus (FLV) leukemogenesis was prevented by treatment of the virus with Concanavalin A (Con A). Mice infected with the lectin-treated virus, however, showed evidence of a dormant infection since infectious virus could be recovered for as long as 100 days. Humoral immune responses to sheep erythrocytes (SRBC), a thymus-dependent antigen, and to E. coli lipopolysaccharide (LPS), a thymus-independent antigen, were depressed (approximately 80-90%) in mice given the Con A-treated FLV. Cell transfer studies indicated that the impaired responsiveness to SRBC was related to a defect in B-lymphocyte function, similar to the impairment in mice infected with untreated FLV. The mitogenic response of splenocytes from Con A-FLV mice to E. coli LPS was also depressed as was the ability of Ig-bearing spleen cells to redistribute these immunoglobulin receptors into polar caps. The impaired immune responsiveness in the Con A-FLV infected mice appeared associated with the persistent virus infection and not to neoplastic transformation generally associated with leukemogenic process.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology*; D004912:Erythrocytes / Q000276:immunology; D005622:Friend murine leukemia virus / Q000187:drug effects / Q000276:immunology; D007165:Immunosuppression Therapy; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000382:microbiology; D013163:Splenomegaly / Q000517:prevention & control; D014412:Tumor Virus Infections / Q000276:immunology* / Q000401:mortality
D000956:Antigens, Viral; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D011947:Receptors, Antigen, B-Cell; D003208:Concanavalin A