Quantitative and immunologic aspects of the handling of 2,4 dinitrophenyl guinea pig albumin by macrophages.
Studies correlating quantitative aspects of the handling of dinitrophenyl guinea pig albumin (DNP-GPA) by guinea pig macrophages with the potential of cell-associated antigen to initiate proliferation of immune T lymphocytes have examined the nature of immunologically relevant antigen. After the loss of 75% of cell-bound DNP-GPA during the first 24 hr of in vitro culture, the remaining antigen persists qualitatively unchanged throughout further culture. However, coincident immunogenicity of the macrophage-associated NDP-GPA progressively deccreases, suggesting loss of accessibility of the antigen to responding immune lymphocytes. There is a small, stable, surface antigen pool but these studies suggest that the immunologically critical fraction of DNP-GPA, as regards guinea pig T cell activation, is resistant to trypsinization and inaccessible to antibody.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002469:Cell Separation; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004140:Dinitrophenols / Q000276:immunology*; D005971:Glutamates; D006168:Guinea Pigs; D006433:Hemocyanins; D007106:Immune Sera; D007114:Immunization; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D008239:Lysine; D008264:Macrophages / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014357:Trypsin
D004140:Dinitrophenols; D005971:Glutamates; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D012709:Serum Albumin; D014316:Tritium; D004247:DNA; D006433:Hemocyanins; D014357:Trypsin; D008239:Lysine; D013936:Thymidine