title: Review of calcium methodologies.
abstract: A review of calcium methodologies for serum has been described. The analytical systems developed over the past century have been classified as to type beginning with gravimetry and extending to isotope dilution-mass spectrometry by covering all of the commonly used technics which have evolved during that period. Screening and referee procedures are discussed along with comparative sensitivities encountered between atomic absorption spectrophotometry and molecular absorption spectrophotometry. A procedure involving a simple direct reaction for serum calcium using cresolphthalein complexone is recommended in which high blanks are minimized by repressing the ionization of the color reagent on lowering the dielectric constant characteristics of the mixture with dimethylsulfoxide. Reaction characteristics, errors which can be encountered, normal ranges and an interpretative resume are included in its discussion.
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002118:Calcium / Q000097:blood*; D011232:Chemical Precipitation; D003408:Cresols; D003956:Dialysis; D005453:Fluorescence; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods; D010635:Phenolphthaleins; D000644:Quaternary Ammonium Compounds; D013053:Spectrophotometry; D013054:Spectrophotometry, Atomic
chemical_list: D003408:Cresols; D007202:Indicators and Reagents; D010635:Phenolphthaleins; D000644:Quaternary Ammonium Compounds; D002118:Calcium