[Synthesis and biological activity of some cystine peptides from the active center of the basic trypsin inhibitor from bovine organs (Kunitz inhibitor) (author's transl)].
The synthesis of the following 3 model peptides from the active center of the inhibitor molecule is described: (see article). The 3 peptides are split by trypsin with velocities increasing from (I) to (III). The following Ki values were determined: (I) Ki = 2.3 x 10(-2)M; (II) Ki = 4.9 x 10(-3)M; (III) Ki = 2.1 x 10(-3)M. These Ki values differ from the Ki values of the Kunitz inhibitor by a factor 10(11) to 10(12).
D000818:Animals; D007611:Aprotinin / Q000378:metabolism*; D002417:Cattle; D007700:Kinetics; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000378:metabolism; D013329:Structure-Activity Relationship; D014357:Trypsin / Q000378:metabolism
D009842:Oligopeptides; D010456:Peptides, Cyclic; D007611:Aprotinin; D014357:Trypsin
Synthese und biologische Aktivatät von Cystinpeptiden aus dem aktiven Zentrum des basischen Trypsininhibitors aus Rinderorganen (Kunitz-Inhibitor)