The purification of lymphocytes in chicken blood.
Several methods have been tried in attempts to purify the lymphocytes of chicken blood.  The most efficient one is based upon the properties of adhesion of the polymorphonuclears and monocytes.  With the use of a column filled with synthetic fibers, held at 37 degrees C., a high rate of lymphocyte purification was obtained.  Sedimentation and centrifugation then resulted in the elimination of almost all of the erythrocytes.  The final product was homogeneous and the lymphocytes obtained kept the physiological and metabolic properties necessary for their culture.
D000268:Adhesiveness; D000818:Animals; D002469:Cell Separation; D002498:Centrifugation; D002645:Chickens / Q000097:blood*; D003584:Cytological Techniques; D004906:Erythrocyte Count; D007958:Leukocyte Count; D008214:Lymphocytes; D013696:Temperature