title: In vitro response of subpopulations of human tonsil lymphocytes. I. Cellular collaboration in the proliferative response to PHA and Con A.
abstract: Human tonsil lymphocytes have been separated into three subpopulations of cells: purified B cells and two subsets of purified T cells (F1 and F2). B cells were obtained by rosetting with neuraminidase treated SRBC. F1 and F2 were separated by filtration on a nylon wool column using different speeds of elution. Purified B cells contained less than 5% T cells, the T cells preparations contained less than 5% B cells for F1 and 10 to 15% for F2, respectively. A significant contamination in cells not identified by any B or T marker was observed in purified B cells and in F1. Adherent cells enhanced the response of each lymphochte population to PHA and Con A. This explained the paradoxically low responsiveness of the purified T cells. Purified B cells did not respond to these mitogens in different culture conditions. However, a small B cell response was observed when they were cultured in the presence of mitomycin-treated T cells. Striking was the enhancing effect of B cells on the T cell response to PHA and Con A. This enhancing effect was observed even when B cells were treated with mitomycin or depleted in adherent cells. The comparison of the F1 and F2 response suggested that they contained distinct types of T cells.
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D004903:Erythrocyte Aggregation; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D008937:Mitomycins / Q000494:pharmacology; D014066:Palatine Tonsil / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D037102:Lectins; D008937:Mitomycins; D003208:Concanavalin A