An enzymatic derivative double isotope assay for measuring tissue levels of taurine.
The development of a new assay for taurine is described. The procedure utilizes the formation of taurocholic acid by rat liver microsomes and is dependent upon the dilution of the specific activity of radioactive taurine by the amount of taurine present in perchloric acid homogenates of tissues. Known amounts of taurine (21/2-300 nmol) are added to incubation vessels to establish a standard curve so that unknown quantities of taurine present in tissue homogenates can be calculated by a graphical comparison. The possible interference by other compounds present in tissue extracts and their removal by passage through columns containing both cation and anion exchange resins is discussed. Conditions are established for the quantitative recovery of taurine after passage through the exchange resins. Measurement of the taurine levels of several rat tissues is presented along with duplicate assays performed on the amino acid analyzer for comparison purposes.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002852:Chromatography, Ion Exchange; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D011865:Radioisotope Dilution Technique / Q000379:methods; D051381:Rats; D013455:Sulfur / Q000494:pharmacology; D013462:Sulfur Radioisotopes; D013654:Taurine / Q000032:analysis* / Q000378:metabolism; D013656:Taurocholic Acid / Q000096:biosynthesis; D014316:Tritium
D000596:Amino Acids; D013462:Sulfur Radioisotopes; D014316:Tritium; D013654:Taurine; D013656:Taurocholic Acid; D013455:Sulfur