Studies on the human T lymphocyte population. III. Synthesis and release of the lymphocyte receptor for sheep red blood cells by stimulated human T lymphoblasts.
The IgG fraction of a goat antibody specific for human T lymphocytes (ATG) and that of a rabbit antibody directed (anti-Ts) were used to study the synthesis, display, and release of the receptor for SRBC (Ts) by normal human peripheral lymphocytes. A large percentage of lymphoblasts obtained by stimulation of lymphocytes with PHA or PWM formed multilayered rosettes with SRBC. The vast majority of these rosetting lymphoblasts were T lymphocyte derived, whereas few B lymphoblasts formed rosettes. Significant increases were found in the quantity of Ts on the lymphoblast membrane and in the supernatants from PHA-stimulated cultures, especially late in transformation. Furthermore, lymphocytes cultured with PHA and 3H-leucine synthesized and released radiolabeled Ts.
D000818:Animals; D000941:Antigens; D001666:Binding Sites, Antibody; D002469:Cell Separation; D002478:Cells, Cultured; D003973:Diatrizoate; D004912:Erythrocytes / Q000276:immunology*; D005362:Ficoll; D005455:Fluorescent Antibody Technique; D006041:Goats / Q000276:immunology; D006801:Humans; D007104:Immune Adherence Reaction; D007106:Immune Sera; D007074:Immunoglobulin G; D037102:Lectins; D007930:Leucine / Q000378:metabolism; D008213:Lymphocyte Activation; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014316:Tritium; D014357:Trypsin
D000941:Antigens; D007106:Immune Sera; D007074:Immunoglobulin G; D037102:Lectins; D014316:Tritium; D003973:Diatrizoate; D005362:Ficoll; D014357:Trypsin; D007930:Leucine