Simplified alpha1-antitrypsin phenotyping by immunofixation of acid-starch gels.
Immunofixation of acid-starch gels represents a simplified method for alpha1-antitrypsin phenotyping. This technique reduces the amount of work and time involved in phenotyping by eliminating the need for crossed-immunoelectrophoresis. Its major purpose is to directly enhance the staining quality of the minor bands, especially the Z bands on the acid-starch gel. It is hoped that this modified method will allow more wide-spread use of antitrypsin phenotyping, especially in the routine clinical chemistry laboratory.
D000906:Antibodies; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D007123:Immunoelectrophoresis, Two-Dimensional; D010641:Phenotype; D000515:alpha 1-Antitrypsin / Q000032:analysis* / Q000276:immunology; D019896:alpha 1-Antitrypsin Deficiency
D000906:Antibodies; D000515:alpha 1-Antitrypsin