Immunofluorescence of soft-tissue tumors with anti-smooth-muscle and anti-skeletal-muscle antibodies.
One hundred thirteen soft-tissue tumors were studied by immunofluorescence using anti-smooth-muscle (SMA) and anti-skeletal-muscle antibodies (MGA). Of the classic leiomyomas examined, 94% manifested bright fluorescence with SMA, whereas cellular leiomyomas and leiomyosarcomas failed to stain. Of the rhabdomyosarcomas studied, 21% showed fluorescent cross-striations or fluorescent granules after incubation with MGA. None of 48 control tumors fluoresced with either antibody. No tumor vessel seen showed smooth-muscle fluorescence in its walls. Although of little diagnostic value, immunofluorescence utilizing specific human antibodies is a valuable tool with which to study some of the antigenic components of these neoplasms.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D002648:Child; D002675:Child, Preschool; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006041:Goats / Q000276:immunology; D006801:Humans; D007223:Infant; D007889:Leiomyoma / Q000276:immunology*; D007890:Leiomyosarcoma / Q000276:immunology*; D008080:Liposarcoma / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000276:immunology*; D009132:Muscles / Q000276:immunology*; D009442:Neurilemmoma / Q000276:immunology*; D012208:Rhabdomyosarcoma / Q000276:immunology*; D012509:Sarcoma / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic