[Passive and active elements of the potassium secretion system in the skin of frogs].
A method has been evolved for studying the potassium and sodium transport without labelled ions in the frog skin. The potassium secretion sharply increased after the nystatinum had been added to the solution near the skin surface. Suppression of the apical membrane sodium permeability with amiloride or triamteren did not alter the potassium secretion velocity. The nystatinum increased both the potassium and sodium permeability. The blocking agents for sodium channels became ineffective in the presence of nystatinum. During the nystatinum action on the apical membrane the work of Na/K pump does not change in the basal plasmatic membrane, while suppression of the pump with strophantinum abolishes the potassium secretion across the apical membrane. Vasopressin and aldosterone increase the potassium secretion. Therefore, the potassium secretion level depends on the apical membrane potassium permeability and is independent of the sodium transport across this membrane, it is due to potassium inflow into the cell with the aid of Na/K pump of the basal plasmatic membrane.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D009761:Nystatin / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D011896:Rana temporaria; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism
D009761:Nystatin; D012964:Sodium; D011188:Potassium
Passivnyĭ i aktivnyĭ élementy sistemy sekretsii kaliia v kozhe liagushki