Antigenic analysis of Chlamydiae by two-dimensional immunoelectrophoresis. I. Antigenic heterogeneity between C. trachomatis and C. psittaci.
Tw0-dimensional immunoelectrophoresis was used for the antigenic analysis of Chlamydia. Antigens of chlamydiae were found to be successfully solubilized with the nonionic detergent Triton X-100. Nineteen and 16 distinct components were observed for Chlamydia trachomatis strain L2/434/Bu and C. psittaci strain Meningopneumonitis/Cal-10, respectively. The triton-solubilized antigens of chlamydiae differ strikingly between species since only one strong reciprocal cross-reacting antigen was observed between 434 and Cal-10. However, the antigens of Trachoma strain K/UW-31/Cx and LGV strain 434 were found to be very similar.
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D015478:Antigens, Heterophile / Q000302:isolation & purification*; D002692:Chlamydia trachomatis / Q000276:immunology*; D002691:Chlamydophila psittaci / Q000276:immunology*; D003429:Cross Reactions; D007106:Immune Sera / Q000302:isolation & purification; D007115:Immunization Schedule; D007123:Immunoelectrophoresis, Two-Dimensional; D011092:Polyethylene Glycols / Q000378:metabolism; D011817:Rabbits
D000942:Antigens, Bacterial; D015478:Antigens, Heterophile; D007106:Immune Sera; D011092:Polyethylene Glycols