title: Response of human lymphocytes to mitogenic stimuli after irradiation in vitro.
abstract: Human lymphocytes were exposed to varying doses of roentgen irradiation in vitro and thereafter tested for reactivity to different polyclonal mitogens and antigens using DNA synthesis as a marker for viability. The dose response profiles obtained indicate that there are two subpopulations of lymphocytes which are responsive to phytohaemagglutinin, poke weed mitogen, concanavalin A and allogeneic cells. One is relatively sensitive to radiation and the other is relatively resistant. However, no "resistant" PPD-tuberculin responsive cell population could be detected. Irradiated lymphocyte populations enriched for T-cells exhibited both a sensitive and a resistant PHA-responsive population, whereas cell populations enriched for B-cells only exhibited a radiation sensitive one.
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000528:radiation effects; D002458:Cell Fractionation; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000528:radiation effects; D008214:Lymphocytes / Q000276:immunology / Q000528:radiation effects*; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology*; D011830:Radiation Effects; D013601:T-Lymphocytes / Q000528:radiation effects; D014373:Tuberculin / Q000494:pharmacology
chemical_list: D037102:Lectins; D008934:Mitogens; D014373:Tuberculin