title: Testing the mutagenic potency of chemical substances in a linear host-mediated assay. I. Experimental microbiological basis.
abstract: By the use of the mutagenic substance hydrazine sulphate it is shown that the currently used single determination method for determining point mutations in host-mediated assays with calculation of mutation frequencies can lead to erroneous results. The microbiological basis for a linear-method is presented in which the population growth of the auxotrophic and substance-induced mutants used int he test can be described mathematically during their logarithmic growth phase with regression lines.
mesh_terms: D000818:Animals; D005827:Genetics, Microbial; D006834:Hydrazines / Q000494:pharmacology; D007538:Isoniazid / Q000494:pharmacology; D008297:Male; D008722:Methods; D051379:Mice; D008967:Molecular Biology; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D011336:Probability; D012486:Salmonella typhimurium / Q000187:drug effects / Q000472:pathogenicity; D012706:Serratia marcescens / Q000187:drug effects / Q000472:pathogenicity; D013997:Time Factors
chemical_list: D006834:Hydrazines; D009153:Mutagens; D007538:Isoniazid