A comparative study of the effects of glutamate and kainate on the lobster muscle fibre and the frog spinal cord.
1 The depolarizing actions of glutamate and its conformationally restricted analogue kainate were investigated on the lobster muscle fibre and the frog spinal cord using intracellular and extracellular recordings, respectively. 2 Bath-applied kainate was less potent than glutamate on the lobster fibre but more potent on the frog cord. From the log-log transformation of dose-response curves it was proposed that more than one glutamate molecule was necessary to activate both the lobster and the frog receptor sites. In the frog, at least three kainate molecules were thought to be required for receptor activation. 3 The ionic dependence of glutamate and kainate responses appeared different for the two tissues. 4 Some possible explanations of the differential tissue sensitivity to kainate are discussed.
D000818:Animals; D005971:Glutamates / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D009132:Muscles / Q000187:drug effects*; D008121:Nephropidae / Q000502:physiology*; D009467:Neuromuscular Depolarizing Agents / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology*; D011896:Rana temporaria; D013116:Spinal Cord / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology
D005971:Glutamates; D009467:Neuromuscular Depolarizing Agents; D011759:Pyrrolidines; D013779:Tetrodotoxin