Transfer ribonucleic acid methylase deficiency found in UGA supressor strains.
Extracts of recessive UGA suppressor strains, designated supK, are deficient in transfer ribonucleic acid (tRNA)-methylating activity when compared to wild-type extracts. Moreover, the tRNA from suppressor strains is methyl deficient when compared to wild-type tRNA. This deficiency is due to the lack of a single tRNA methylase activity in suppressor strains. UGA suppressor activity may be caused by the miscoding of one or more methyl-deficient tRNA's in supK strains.
D002474:Cell-Free System; D005808:Genes, Recessive; D005815:Genetic Code; D008745:Methylation; D009154:Mutation; D012329:RNA, Bacterial / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism; D012486:Salmonella typhimurium / Q000201:enzymology* / Q000378:metabolism; D013489:Suppression, Genetic; D012359:tRNA Methyltransferases / Q000096:biosynthesis* / Q000378:metabolism
D012329:RNA, Bacterial; D012436:S-Adenosylmethionine; D012343:RNA, Transfer; D012359:tRNA Methyltransferases