title: T and B lymphocytes and Reed-Sternberg cells in Hodgkin's disease lymph nodes and spleens.
abstract: Lymphoid cells from twenty-four untreated Hodgkin's disease biopsies were examined for spontaneous sheep erythrocyte and sensitized ox erythrocyte rosette formation for the identification of T cell and cells with Fc and C3 receptors and surface immunoglobulin. Compared with normal tissues mean T-lymphocytes values were elevated in both involved lymph nodes and uninvolved spleens from Hodgkin's patients. Lymphocytes bearing C3 receptors were correspondingly reduced in these tissues. Involved spleen T-cell values fell within the normal range. In normal tissues the sum of lymphocytes with surface immunoglobulin and sheep erythrocyte receptors fell in the range 89-108%. In six biopsies of Hodgkin's tissue the sum was outside the normal range (121-142%). This observation is compatible with surface immunoglobulin-coated T cells. Surface marker characteristics and intracellular immunoglobulin studies of small lymphocytes, lymphoblasts and Hodgkin's cells suggested that the neoplastic cells were of B lymphocyte origin.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001402:B-Lymphocytes; D001706:Biopsy; D003176:Complement C3; D005260:Female; D006644:Histiocytes; D006689:Hodgkin Disease / Q000473:pathology*; D006801:Humans; D007104:Immune Adherence Reaction; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D008875:Middle Aged; D011947:Receptors, Antigen, B-Cell; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes
chemical_list: D003176:Complement C3; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011947:Receptors, Antigen, B-Cell