Occurrence of polyamines in coliphages T5, phiX174 and in phage-infected bacteria.
The polyamine spermidine and the diamine putrescine have been detected in coliphages T5 and phiX174. Polyamines were identified by thin-layer chromatography and mass-spectrometry of dansyl derivatives, as well as by ion-exchange chromatography. In phiX174 phages, polyamines were sufficient to neutralize 0.5% of DNA phosphates. The polyamine content of T5 phages depended on growth media and purification procedures, but at least 1% of DNA phosphates were neutralized by polyamines. After infection, an increase in cellular polyamine was noticed. This increase paralleled variations in ornithine decarboxylase activity.
D002262:Carboxy-Lyases / Q000378:metabolism; D003090:Coliphages / Q000032:analysis* / Q000254:growth & development; D004267:DNA Viruses; D004269:DNA, Bacterial / Q000096:biosynthesis; D004279:DNA, Viral / Q000032:analysis; D003619:Dansyl Compounds; D004926:Escherichia coli / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism; D009952:Ornithine / Q000378:metabolism; D010710:Phosphates / Q000032:analysis; D011073:Polyamines / Q000032:analysis* / Q000096:biosynthesis; D011700:Putrescine / Q000032:analysis; D013095:Spermidine / Q000032:analysis
D004269:DNA, Bacterial; D004279:DNA, Viral; D003619:Dansyl Compounds; D010710:Phosphates; D011073:Polyamines; D009952:Ornithine; D002262:Carboxy-Lyases; D013095:Spermidine; D011700:Putrescine