Effects of treating immune lymphocytes with a proteolytic enzyme (papain): reduction in immune potential and recovery after incubation.
Immune spleen cells were treated with papain, washed, and boosted with homologous antigen (4-hydroxy-3-iodo-5-nitrophenylacetic acid coupled to chicken globulin) for 1 hr at 4 degrees C. When transferred to irradiated mice, they produced up to 20-fold less anti-hapten antibody than non-papain-treated cells. The lymphocytes could recover their ability to respond if incubated at 37 degrees C after papain treatment but before antigen boosting. Incubation for 30 min at 37 degrees C gave complete or almost complete recovery. Although papain usually reduced the response, in 31% of experiments the reduction was not significant. Possible reasons for this variability are discussed.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000917:Antibody Formation / Q000187:drug effects*; D002478:Cells, Cultured; D003864:Depression, Chemical; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008198:Lymph Nodes; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009611:Nitroxinil / Q000494:pharmacology; D010206:Papain / Q000008:administration & dosage / Q000494:pharmacology*; D013154:Spleen; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009611:Nitroxinil; D010206:Papain