Lymphokine production by C3b-stimulated B cells.
Trypsin cleavage of guinea pig C3 (60 sec, 20 degrees C) yields a fragment which stimulates guinea pig lymphocytes to produce a chemotactic lymphokine. These digested C3 preparations possess only minimal inherent chemotactic properties. The response is completely inhibited by prior adsorption of the trypsin-derived C3 fragments with insolubilized anti-C3. The active C3 fragment is C3b as demonstrated by the elution of activity in the exclusion volume of Sephadex G-75 (m.w. greater than 50,000 dalton). The C3b-dependent cellular-derived chemotactic factor is present in the supernatants of B cell cultures and totally absent in those of T cell cultures. Generation of this lymphokine occurs in the absence of cellular proliferation.
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002021:Buffers; D002633:Chemotaxis / Q000187:drug effects; D002850:Chromatography, Gel; D003176:Complement C3 / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism*; D006041:Goats / Q000276:immunology; D006168:Guinea Pigs; D007106:Immune Sera / Q000302:isolation & purification; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000302:isolation & purification; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000096:biosynthesis*; D009000:Monocytes / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000378:metabolism
D002021:Buffers; D003176:Complement C3; D007106:Immune Sera; D007074:Immunoglobulin G; D037102:Lectins; D008070:Lipopolysaccharides; D008222:Lymphokines; D014361:Trypsin Inhibitors; D003165:Complement System Proteins; D014357:Trypsin