title: Cell numbers in human lymphocyte cultures stimulated with pokeweed mitogen.
abstract: As determined by electronic cell counting, the cell numbers in pokeweed mitogen (PWM) stimulated cultures of normal lymphocytes decreased by about 13% during the first day and then remained almost constant up to day 8. In contrast, a progressive decrease of the cell count was observed in cultures of chronic lymphocytic leukaemia (CLL) lymphocytes reaching about 40% of the initial number on day 8. In PWM cultures of normal lymphocytes the transformed cells increased to about 20% of the cells present on day 4, whereas in cultures of CLL lymphocytes these cells reached only 11% on day 7.
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood*; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008934:Mitogens; D013601:T-Lymphocytes / Q000276:immunology; D013936:Thymidine / Q000378:metabolism
chemical_list: D008934:Mitogens; D004247:DNA; D013936:Thymidine