The effects of U.V.-light, ionizing radiation and the carcinogen N-acetoxy-2-fluorenylacetamide on the development in vitro of one- and two-cell mouse embryos.
The development of one- and two-cell mouse embryos to morula-blastula stages was followed in vitro after treatment with low doses of U.V.-light, ionizing radiation or N-acetoxy-2-fluorenylacetamide. Exposure of one-cell embryos to either radiation source 18 and 24 hours after human chorionic gonadotropin injections prevented maturation, most embryos being arrested at the one-cell stage and a few at the two-cell stage. Two-cell embryos, however, were not sensitive to low doses of either U.V. or X-irradiation and developed normally. Treatment of early one-cell embryos with the carcinogen, N-acetoxy-2-fluorenyl-acetamide (0-7 muM), also arrested development, whereas exposure of late one-cell embryos did not completely prevent maturation to morula-blastula stages. Exposure of two-cell embryos to the same concentration of carcinogen had no effect on their development to blastulas. Results with all three agents showed that mouse embryos at the one-cell stage are more sensitive than those at the two-cell stage, as judged by their ability to develop in vitro.
D000099:Acetoxyacetylaminofluorene; D000818:Animals; D002273:Carcinogens; D005260:Female; D005449:Fluorenes; D066298:In Vitro Techniques; D051379:Mice; D011836:Radiation Tolerance; D014466:Ultraviolet Rays; D014965:X-Rays; D015053:Zygote / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects
D002273:Carcinogens; D005449:Fluorenes; D000099:Acetoxyacetylaminofluorene