title: Studies on the immunizing capacity of orally administered particulate antigens. II. Production of immunological memory in germ-free mice by orally administered sheep erythrocytes.
abstract: A study was perfromed to find out, whether or not the oral administration of sheep erythrocytes results in a general primary immune reaction as well as in effective priming for the secondary response in both conventional and germ-free NMRI mice. Whereas negative results were obtained with conventionally held mice, five oral applications of 0.3 ml of a 60% suspension of sheep erythrocytes to germ-free mice, each dose separated by an interval of 24 hr, resulted in a general primary immune response both at the cellular and humoral levels. When such pretreated mice were given an i.p. injection of 4 times 10(8) sheep erythrocytes as a secondary antigenic stimulus 32 days after the last of the five oral applications, the subsequent response was characterized by the predominant development of 7S hemolysin-producing spleen cells. This evidently indicates that effective priming for the secondary response has taken place by the orally administered antigen.
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005856:Germ-Free Life; D006462:Hemolytic Plaque Technique; D007114:Immunization; D007115:Immunization Schedule; D007117:Immunization, Secondary; D007070:Immunoglobulin A / Q000032:analysis; D007156:Immunologic Memory / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000276:immunology
chemical_list: D000941:Antigens; D007070:Immunoglobulin A