Single step separation of human T and B cells using AET treated srbc rosettes.
A technique for the single step separation of human thymus derived (T) and Bursa equivalent (B) lymphocytes was developed. Density separation of B lymphocytes and T lymphocyte-sheep red blood cell (SRBC) rosettes on Ficoll-Hypaque was modified by pretreatment of the SRBC with 2 aminoethylisothiouronium bromide hydrobromide. This modification yielded significantly purer populations of T and B cells. Up to 15 X 10(7) were separated without increasing contamination allowing for the recovery of B lymphocytes as well as T lymphocytes in sufficient numbers to use in functional assays.
D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D002498:Centrifugation; D004912:Erythrocytes / Q000187:drug effects; D006801:Humans; D007104:Immune Adherence Reaction; D013601:T-Lymphocytes / Q000276:immunology*; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology*
D000338:beta-Aminoethyl Isothiourea