LDH isozymes of human T and B lymphocytes.
Peripheral lymphocytes were obtained from five normal healthy adults. T and B lymphocytes were separated by rosette formation. LDH (L-lactate: NAD-+ oxidoreductase, EC 1.1.1.27) isozymes were studied on both populations after agar gel electrophoresis. The mean B lymphocyte pattern was: LDH-1, 7.2%; LDH-2, 25.0%; LDH-3, 39.2%; LDH-4, 21.9%; LDH-5, 6.6%. The mean T lymphocyte pattern was: LDH-1, 25.8%; LDH-2, 35.1%; LDH-3, 28.3%; LDH-4, 9.4%, LDH-5, 1.3%. This makes the T lymphocytes look more aerobic than the B lymphocytes. Daudicells had the following pattern: LDH-1, 8.4%; LDH-2, 19.1%; LDH-3, 26.6%, LDH-4, 24.9%; LDH-5, 20.9%.
D000328:Adult; D000362:Agar; D001402:B-Lymphocytes / Q000201:enzymology*; D002449:Cell Aggregation; D004586:Electrophoresis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D013601:T-Lymphocytes / Q000201:enzymology*
D007527:Isoenzymes; D000362:Agar; D007770:L-Lactate Dehydrogenase