title: Progesterone-receptor mediation of chromatin RNA transcription in a cell-free system.
abstract: The chick oviduct progesterone receptor has been purified to homogeneity by affinity chromatography and its molecular action studied in vitro. The native receptor is a 200,000 MW dimer of two dissimilar 4S subunits with different intranuclear function. The receptors directly regulate RNA chain initiation sites in oviduct chromatin by interactions involving target tissue nuclear acceptor sites. There is a 1:1 correspondence between receptor "acceptor" sites and RNA sites. Only the dimer form of the receptor is active in vitro on chromatin templates. The study suggests a novel model for homone action that can be tested directly in this system.
mesh_terms: D000645:Ammonium Sulfate; D000818:Animals; D002474:Cell-Free System; D002642:Chick Embryo; D002843:Chromatin / Q000378:metabolism*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003593:Cytoplasm / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D010057:Oviducts / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis* / Q000302:isolation & purification; D011980:Receptors, Progesterone / Q000187:drug effects*; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D011980:Receptors, Progesterone; D012313:RNA; D000645:Ammonium Sulfate