A method for determining the utilisation of glucose by mycoplasmas.
A technique for measuring glucose utilisation by mycoplasmas, in which the disappearance of glucose is monitored by the glucose-oxidase method, is proposed as an alternative to the pH method. For maximum reliability the number of organisms in the test suspension must exceed a critical minimum value. Differences in efficiency of glucose utilisation were demonstrated between species of Mycoplasma and Acholeplasma.
D000127:Acholeplasma / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005947:Glucose / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism; D009174:Mycoplasma / Q000378:metabolism*; D009176:Mycoplasma mycoides / Q000378:metabolism; D013045:Species Specificity
D005949:Glucose Oxidase; D005947:Glucose