Induction of theta-positive lymphocytes and lymphoblasts in mouse bone marrow by mitogens.
With a sensitive technique for the identification of lymphocytes and lymphoblasts bearing the surface antigens phi, TL, and immunoglobulin, the induction of phi-positive small lymphocytes in bone marrow could be observed. Dibutyryl cyclic adenosine 3',5'-monophosphate and thymosin were both effective in inducing precursor cells to express phi antigen. Concanavalin A and bacterial lipopolysaccharide were also shown to be inducers. After treatment with concanavalin A but not with lipopolysaccharide, lymphoblasts developed in marrow cultures which were positive for phi and TL antigens; when spleen was cultured with concanavalin A, lymphoblasts were phi-positive and TL negative. It is concluded that the cell responsive to concanavalin A (and phytohemagglutinin) in bone marrow is a phi-negative precursor (stem) cell, which is induced first to express phi and TL antigens, and then to differentiate to a lymphoblast.
D000818:Animals; D000941:Antigens / Q000032:analysis; D000961:Antilymphocyte Serum; D001853:Bone Marrow / Q000276:immunology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D005269:Femur; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells / Q000276:immunology; D007111:Immunity, Cellular; D007136:Immunoglobulins / Q000032:analysis; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008808:Mice, Inbred CBA; D008934:Mitogens; D013601:T-Lymphocytes / Q000276:immunology*; D013977:Tibia
D000941:Antigens; D000961:Antilymphocyte Serum; D007136:Immunoglobulins; D008934:Mitogens