Capacity of sensitized thymus-derived lymphocytes to induce fatal lymphocytic choriomeningitis is restricted by the H-2 gene complex.
Adoptive immunization of syngeneic, immunosuppressed recipients infected with lymphocytic choriomeningitis (LCM) virus causes fatal neurologic disease within 2 to 4 days of cell transfer, providing that donors are sampled when the in vitro 51-Cr release assay shows maximal specific activity of sensitized thymus-derived lymphocytes (T cells). Prior treatment of immune spleen cells with AKR anti-omicron ascitic fluid and complement causes total abrogation of this in vivo activity. Fatal neurologic disease is induced only when donor and recipient share at least one set of H-2 antigenic specificities. Parent yields F1 and F1 yields parent combinations are as effective as syngeneic systems, but mice given allogeneic immune cells survive as long as controlsmdifferences at the M-locus in H-2 compatible mice do not inhibit effector activity. Homing of transferred lymphocytes to spleen is similar in syngeneic or allogeneic recipients, but only syngeneic immune cells cross the blood-cerebrospinal fluid (CSF) barrier and cause choriomeningitis. Fatal LCM, is, therefore, apparently induced by a specifically sensitized omicron-bearing cell population, activity of which is restricted by the H-2 gene complex.
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D001921:Brain; D002555:Cerebrospinal Fluid / Q000276:immunology; D002860:Chromium Radioisotopes; D046508:Culture Techniques; D003520:Cyclophosphamide / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D006649:Histocompatibility Antigens; D007112:Immunity, Maternally-Acquired; D007116:Immunization, Passive; D007165:Immunosuppression Therapy; D008216:Lymphocytic Choriomeningitis / Q000276:immunology*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D014776:Virus Cultivation
D002860:Chromium Radioisotopes; D006649:Histocompatibility Antigens; D003520:Cyclophosphamide