title: Microvascular changes in lymph nodes draining skin allografts.
abstract: Histological, histochemical, ultrastructural, and radiolabeling characteristics of the microvasculature in regional nodes draining skin allograft sites are described. From 12 to 48 hours after grafting, these nodes show increased vascular permeability and altered lymphocyte traffic pattern. The rapid rise in lymphocyte migration indices and the apparent plugging of intermediate sinuses by lymphocytes suggest that both increased entry and decreased egress of recirculating cells contribute in "lymphocyte trapping." This is followed by redistribution of cortical capillary arcades as existing germinal centers dissolve and proliferating lymphocytes infiltrate the cortex. Normal microvascular patterns reappeared at 7 to 14 days as primary and secondary nodules form in the enlarged nodes. Increased length and arborization of high endothelial venules resulted from focal proliferation of endothelial cells in transition zones from high to low endothelium. In stimulated nodes, high endothelial cells exhibit increased cytoplasmic basophilia and acid hydrolase activities which correlate with the appearance of numerous polyribosomes, RER cisternae, and lysosomes in their cytoplasm. These "activated" endothelial cells phagocytose microthrombi within venular lumens.
mesh_terms: D000818:Animals; D002199:Capillary Permeability; D002465:Cell Movement; D004727:Endothelium / Q000166:cytology; D008198:Lymph Nodes / Q000098:blood supply* / Q000201:enzymology / Q000648:ultrastructure; D008214:Lymphocytes / Q000276:immunology / Q000648:ultrastructure; D008297:Male; D008833:Microcirculation; D010587:Phagocytosis; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D016038:Skin Transplantation; D013997:Time Factors; D014184:Transplantation, Homologous