Immunoglobulin binding factor (IBF) produced by T cells: selective interaction with slow migrating rabbit IgC.
Antigen-stimulated T lymphocytes release a variety of soluble factors among which an Immunoglobulin Binding Factor (IBF). We report a method of titration of IBF making use of its capacity to inhibit complement-dependent hemolysis of sheep erythrocytes sensitized with rabbit IgG antibodies. Using this technique we have investigated the specificity of IBG for rabbit immunoglobulins fractionated by DEAE-cellulose chromatography. The interaction of IBF with IgG is found to be restricted to the slowest migrating fractions of rabbit IgG.
D000818:Animals; D001666:Binding Sites, Antibody; D003165:Complement System Proteins / Q000378:metabolism; D006461:Hemolysis; D007074:Immunoglobulin G / Q000378:metabolism*; D007075:Immunoglobulin M; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003165:Complement System Proteins