An improved method for the isolation of surface membranes from cells in monolayer culture.
A modification of the procedure reported by Scher and Barland (Biochim. Biophys. Acta 255 (1972) 580-588) for the isolation of the surface membranes of cells grown in monolayer culture is described. The modification involves addition of a purification step utilizing the two-phase polymer system described by Brunette and Till (J. Membr. Biol. 5 (1971) 215-224). The modified method permits the isolation of surface membranes from cells not yet at confluence, when they may easily become detached from the substratum.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000032:analysis / Q000648:ultrastructure*; D002478:Cells, Cultured / Q000648:ultrastructure*; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D005069:Evaluation Studies as Topic; D005347:Fibroblasts / Q000032:analysis / Q000648:ultrastructure; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D012313:RNA / Q000032:analysis
D012313:RNA; D004247:DNA