Role of 11 beta-hydroxyprogesterone in biosynthesis of corticosterone from progesterone in guinea pig adrenals.
Slices of male guinea pig adrenals were incubated with trace amount of 3-H7 alpha-progesterone and the percentage of incorporation of 3-H into various steroids at various intervals was measured. It was found that 3-H accumulates predominately in 11 beta-hydroxyprogesterone as compared to DOC. This indicates that, in guinea pig adrenals, 11 beta-hydroxyprogesterone plays a dominant role in a formation of corticosterone from progesterone. The observed sequence of changes in progesterone label accumulation in 17-hydroxycompounds indicates they pathway of cortisol synthesis through the intermediate formation of 17-hydroxyprogesterone and 11-deoxycortisol.
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D003345:Corticosterone / Q000096:biosynthesis*; D006168:Guinea Pigs; D006854:Hydrocortisone / Q000096:biosynthesis; D006900:Hydroxylation; D006908:Hydroxyprogesterones / Q000378:metabolism*; D008297:Male; D011374:Progesterone / Q000378:metabolism*; D014316:Tritium
D006908:Hydroxyprogesterones; D014316:Tritium; D011374:Progesterone; D003345:Corticosterone; D006854:Hydrocortisone