title: [Mucous (M) mutants of enteropathogenic, septicemic and saprophytic E. coli (author's transl)].
abstract: Mucoid M mutants of enteropathogenic (for suckling pigs), septicemic (for calves), and saprophytic E. Coli were subject of the study. The characteristics of the M colonies have been listed in Table 1. Both spontaneously occurring M mutants which had been isolated by culturing of E. coli strains (S form) in normal horse serum (undiluted) for several weeks were observed. Enzymatic-biochemical tests revealed the existence of M mutants with full enzmatic activity which are identical with the homologous S and R forms. There were also M mutants exhibiting a considerably reduced enzymatic activity. Antigenic-serological analysis (agglutination) exhibited M mutants which were agglutinated by the homologous sera, at low and high titres. In contrast to this, one group of M mutants was found not to be agglutinable, despite autoclaving at 120 degrees C for 2 hours. Some mutants cultured in broth for several weeks were re-exhibiting S colonies of full biochemical activity and full antigen reactivity against homologous agglutinating sera.
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial; D050260:Carbohydrate Metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D002417:Cattle; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D006461:Hemolysis; D006736:Horses; D007106:Immune Sera; D007211:Indoles / Q000378:metabolism; D009154:Mutation; D018805:Sepsis / Q000382:microbiology; D013045:Species Specificity; D013552:Swine
chemical_list: D000942:Antigens, Bacterial; D003470:Culture Media; D007106:Immune Sera; D007211:Indoles; D002262:Carboxy-Lyases
vernacular_title: Die mukösen (M)-Mutanten der enteropathogenen, septikämischen und saprophytären Escherichia coli