Partial purification and characterization of a lymphotoxic factor from multiple sclerosis serum.
A factor found in the serums of some patients with multiple sclerosis has been shown to have a toxic effect on the normal, mature lymphocyte. This lymphotoxic factor is a protein that is stable to heating at 56 degrees C for 3 hours. It is a nondialyzable material, with recovery of lymphotoxic activity following 30 days of dialysis. Elution of lymphotoxic serum through Sepadex G-100 demonstrates toxic activity closely associated with albumin. Purification by elution through DEAE-cellulose yielded lymphotoxic activity. The lymphotoxic factor was inactivated by standard ammonium sulfate precipitation. Analysis of the effect on distinctive cell types within the total lymphocyte population demonstrated that the T lymphocyte was particularly sensitive.
D000645:Ammonium Sulfate; D000961:Antilymphocyte Serum / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D001798:Blood Proteins / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003601:Cytotoxicity Tests, Immunologic; D003956:Dialysis; D006358:Hot Temperature; D006801:Humans; D009103:Multiple Sclerosis / Q000097:blood* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000961:Antilymphocyte Serum; D001798:Blood Proteins; D000645:Ammonium Sulfate