title: Effect of acetone fixation on infectivity and antigenicity of respiratory syncytial virus and adenovirus in the fluorescent antibody test.
abstract: An investigation was made on the effect of acetone fixation on infectivity and immunofluorescent antigenicity of respiratory syncytial virus and adenovirus type 5, lipid- and nonlipid-containing viruses, respectively. Viruses were allowed to replicate in HEp-2 cells, and the cells were then fixed in acetone at 5 C for periods ranging from 30 s to 7 days. Treatment for 10 min was sufficient to inactivate respiratory syncytial virus, whereas infectious adenovirus type 5 could be isolated from cells immersed in acetone for 7 days. There was a gradual reduction, to 50% of that observed at 30 s, in the intensity of fluorescent antibody staining of both viruses with increasing fixation time, but no significant decreases in fluorescent antibody end-point titers of antisera to either virus were observed.
mesh_terms: D000096:Acetone / Q000494:pharmacology*; D000256:Adenoviridae / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D000956:Antigens, Viral; D002460:Cell Line; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D012136:Respiratory Syncytial Viruses / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D000096:Acetone