Chromatin directed transcription of 5S and tRNA genes.
Chromatin prepared by gentle methods from mouse myeloma cells retained its ability to synthesize RNA using bound endogenous RNA polymerase (RNA nucleotidyltransferase; nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6). The transcription resembles that observed in vivo in several respects. The low-molecular-weight RNA species 5S RNA and the 4.5S precursor to 4S RNA, are transcribed accurately and transcription is reinitiated continually in vitro. Their synthesis was not inhibited by alpha-amanitin (1 mug/ml) as was found previously for these species in isolated nuclei.
D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D008970:Molecular Weight; D009194:Myeloma Proteins; D009693:Nucleic Acid Hybridization; D009694:Nucleic Acid Precursors / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014981:Xenopus
D002843:Chromatin; D004273:DNA, Neoplasm; D009194:Myeloma Proteins; D009694:Nucleic Acid Precursors; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D012343:RNA, Transfer; D012321:DNA-Directed RNA Polymerases