Effect of temperature on ergosterol biosynthesis in yeast.
Anaerobically grown Saccharomyces cerevisiae was aerated for 7 hr at 20 degrees, 30 degrees, or 40 degrees, in a phosphate buffer containing 2% glucose. At elevated temperature (40 degrees), de novo synthesis of squalene and sterois in the aerated yeast was only 32-35% of that at lower temperature (20 degrees or 30 degrees), and this decrease was attributed to the repression of the enzymes involved in the synthesis of mevalonate from acetyl-COA. In addition, at elevated temperature, the metabolic flux from squalene to ergosterol was blocked at squalene epoxidation, lanosterol demethylation, and ergosta-5, 7, 22, 24(28)-tetraene-3beta-ol reduction.
D000105:Acetyl Coenzyme A / Q000378:metabolism; D004875:Ergosterol / Q000096:biosynthesis*; D008055:Lipids / Q000096:biosynthesis; D008780:Methyltransferases / Q000378:metabolism; D008798:Mevalonic Acid / Q000378:metabolism; D010100:Oxygen; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013185:Squalene / Q000096:biosynthesis; D013261:Sterols / Q000096:biosynthesis; D013696:Temperature
D008055:Lipids; D013261:Sterols; D000105:Acetyl Coenzyme A; D013185:Squalene; D008780:Methyltransferases; D008798:Mevalonic Acid; D010100:Oxygen; D004875:Ergosterol