The induction and regulation of guinea pig B-lymphocyte proliferation in vitro.
Conditions for the in vitro induction of proliferation have been examined in a population of guinea pig lymph node lymphocytes enriched for B lymphocytes. Known B cell mitogens such as bacterial lipopolysaccharide and tuberculin-purified protein derivative induced DNA synthetic responses in this B lymphocyte population as did a number of dinitrophenyl hapten-protein conjugates, all in the apparent absence of T cell participation. B cell proliferation occurred most efficiently in serum-free medium with both heterologous and homologous sera inhibiting maximum responses. In vivo immunization with hapten-protein conjugates failed to enhance subsequent in vitro B cell responsiveness to these materials beyond that owing to recruitment by contaminating antigen-primed T cells. Macrophage-associated antigen, which efficiently triggered T cell proliferation was much less effective at initiating B cell DNA synthesis than was soluble antigen not bound to macrophages. Furthermore, although accessory cells were required for the development of T cell DNA synthetic responses, macrophages of factors produced by them inhibited spontaneous or induced B cell 3H-thymidine incorporation.
D000818:Animals; D000941:Antigens; D001202:Ascitic Fluid / Q000166:cytology; D001402:B-Lymphocytes / Q000276:immunology*; D001500:BCG Vaccine / Q000494:pharmacology; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006168:Guinea Pigs; D008213:Lymphocyte Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000494:pharmacology
D000941:Antigens; D001500:BCG Vaccine; D011135:Polysaccharides, Bacterial; D004247:DNA