Cellular location of alpha-hemolysin in Staphylococcus aureus.
Protoplast fractionation techniques were used to prepare subcellular fractions of three strains of Staphylococcus aureus for the analysis of alpha-hemolysin activity. More than 90% of the cellular hemolytic activity was localized in the periplasm, only a trace was detected in the cytoplasm, and none was found in either plasma membranes or mesosomes. This cellular portion constituted approximately 4 to 10% of the total hemolytic activity of the culture. The erythrocyte lysis spectrum and Sephadex G-100 filtration studies indicated that the cellular hemolysin closely resembles the extracellular form.
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001431:Bacteriological Techniques; D002845:Chromatography; D004269:DNA, Bacterial / Q000032:analysis; D003911:Dextrans; D004912:Erythrocytes / Q000276:immunology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006460:Hemolysin Proteins / Q000032:analysis*; D006461:Hemolysis; D011523:Protoplasts; D011817:Rabbits / Q000276:immunology; D013210:Staphylococcus / Q000276:immunology*; D013347:Subcellular Fractions
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D003911:Dextrans; D006460:Hemolysin Proteins; D005954:Glucosephosphate Dehydrogenase