title: A simultaneous method for detection and quantitation of T- and B-lymphocytes.
abstract: Assay for quantitation of B- and T--lymphocyted from small volumes of heparinized man blood has been developed by simultaneously reacting Ficoll-Hypaque purified mononuclear cells with antobody-coated chicken erythrocytes to for rosettes with B-lymphocytes, and sheep erythrocytes which formed non-immune rosettes with Tlymphocytes. Distribution of various lymphocyte populations in normals are T-cells, 60.9+/-7%, B-cells 16.4+/-4%, and lymphocytes with both receptors, 1.6+/-0.6%.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001665:Binding Sites; D002469:Cell Separation; D002645:Chickens / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007104:Immune Adherence Reaction; D007106:Immune Sera; D008722:Methods; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D007106:Immune Sera