Contractile proteins. Major components of nuclear and chromosome non-histone proteins.
Two of the major non-histone proteins from Physarum polycephalum have been isolated under nondenaturing conditions and identified as actin and myosin. A third protein has been purified from crude nuclear actomyosin and from residual nonhistone fractions and found to bind actomyosin in the presence of Mg2+. In Physarum these proteins are not components of the nuclear membrane. Based on sodium dodecylsulfate-polyacrylamide gel electrophoresis, similar proteins are also present in nuclei of HeLa cells and mouse embryo fibroblasts. Isolated metaphase chromosomes from Physarum show a several-fold enrichment in myosin and an altered ratio of actin to the Mg2+-dependent actomyosin binding protein as compared to interphase nuclei. When non-proliferative states are induced in any of these cells, the Mg2+-dependent actomyosin binding protein decreases while actin increases several fold in intranuclear concentration; concomitantly, there is a generalized condensation and inactivation of chromatin. Experiments with added purified radioactive nuclear actomyosin; comparative studies on nuclear protein during stepwise nuclear purification; and studies on isolated metaphase chromosomes indicate that these proteins exist in nuclei in vivo. These observations suggest that contractile proteins may function in the structural interconversions of chromatin and in the regulation of cell proliferation;
D000199:Actins; D000205:Actomyosin / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis* / Q000648:ultrastructure; D002843:Chromatin / Q000032:analysis*; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000032:analysis; D006367:HeLa Cells / Q000032:analysis; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D009132:Muscles / Q000032:analysis / Q000648:ultrastructure; D009218:Myosins; D009235:Myxomycetes / Q000032:analysis*; D009698:Nucleoproteins; D011487:Protein Conformation; D011817:Rabbits; D014357:Trypsin
D000199:Actins; D000596:Amino Acids; D002843:Chromatin; D009698:Nucleoproteins; D000205:Actomyosin; D014357:Trypsin; D009218:Myosins; D008274:Magnesium