Use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus.
A restriction endonuclease from Haemophilus influenzae (Hind III) specifically cleaved vaccinia DNA into 14 fragments. The molecular weights of these fragments were determined by gel electrophoresis and ranged from 0.5 x 10(6) to 30 x 10(6). Hind III digestion of the DNA from the WR and CV-1 strains of vaccinia revealed a small molecular difference in one of the resulting fragments. The average molecular weight of the entire vaccinia genome was calculated to be 125 x 10(6).
D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis*; D004720:Endonucleases / Q000378:metabolism*; D014644:Genetic Variation; D006193:Haemophilus influenzae / Q000201:enzymology; D008970:Molecular Weight; D014616:Vaccinia virus / Q000032:analysis* / Q000254:growth & development
D004279:DNA, Viral; D004720:Endonucleases; D004262:DNA Restriction Enzymes