tRNA tertiary structure in solution as probed by the photochemically induced 8-13 cross-link.
The conformation of ten purified tRNAs from Escherichia Coli has been investigated by means of the photo-induced cross-linking of 4Srd8 and Cyd13, which is sensitive to the juxtaposition of the two bases. Three tRNAs photo-react abnormally slowly; tRNAPhe, tRNAMet/m a and tRNAVal/2; a comparison with normally reacting species suggests that base 47 (Urd or modified Urd) is involved in a tertiary interaction in Class I tRNAs with the triplet 8, 14, 28. The UGA suppressor tRNATrp photoreacts significantly slower than the wild type. Thus the single base change Gua 24 to Ade induces a conformational change that alters the rate constant for the cross-linking reaction.
D001483:Base Sequence; D001665:Binding Sites; D004926:Escherichia coli / Q000032:analysis; D007700:Kinetics; D008433:Mathematics; D008715:Methionine; D009690:Nucleic Acid Conformation; D010649:Phenylalanine; D010777:Photochemistry; D012329:RNA, Bacterial; D012343:RNA, Transfer; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan; D014633:Valine
D012329:RNA, Bacterial; D010649:Phenylalanine; D014364:Tryptophan; D012343:RNA, Transfer; D008715:Methionine; D014633:Valine