DNA synthesis involving a complexes form of DNA polymerase I in extracts of Escherichia coli.
DNA polymerase I (EC 2.7.7.7; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase) has been recovered as a complex of about 390,000 molecular weight. The complex displays an ATP-stimulated DNA-synthesizing activity that prefers native to heat-denatured DNA. Genetic evidence indicates that the recBC enzyme is associated with the polymerase in the complex. Preliminary evidence for complexes involving DNA polymerases II and III is also presented.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D002850:Chromatography, Gel; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D005827:Genetics, Microbial; D008970:Molecular Weight; D009154:Mutation; D009691:Nucleic Acid Denaturation; D011189:Potassium Chloride / Q000494:pharmacology; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology
D004269:DNA, Bacterial; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D004254:DNA Nucleotidyltransferases