Fluorescamine as a histochemical reagent: demonstration of polypeptide hormone-secreting cells.
Fluorescamine is a useful fluorescence microscopic reagent for the demonstration of certain peptide hormone-secreting cells in formaldehyde-fixed tissues. Among the cells demonstrated are the pituitary GH cells, the gastrin cells, the insulin cells and the thyroid C cells. In the latter cell system degranulation brings about a marked decrease in the intensity of the fluorescamine-induced fluorescence suggesting that the fluorogenic component is associated with the secretory granules. In models neither of the hormones tested--GH, gastrin, insulin--gave fluorescamine-induced fluorescence after pretreatment with formaldehyde. It is suggested that the fluorescamine-induced fluorescence of the endocrine cells demonstrates granular components distinct from the hormones.
D000818:Animals; D001001:Anura; D002415:Cats; D002645:Chickens; D006224:Cricetinae; D004285:Dogs; D005450:Fluorescamine; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D005755:Gastrins / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism; D006168:Guinea Pigs; D006728:Hormones / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D008856:Microscopy, Fluorescence / Q000379:methods*; D010179:Pancreas / Q000166:cytology; D010902:Pituitary Gland / Q000166:cytology; D011817:Rabbits; D051381:Rats; D013270:Stomach / Q000166:cytology; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology
D005755:Gastrins; D006728:Hormones; D007328:Insulin; D005557:Formaldehyde; D005450:Fluorescamine; D013006:Growth Hormone