IgG antilymphocyte antibodies in SLE detected by 125I protein A.
125I-labeled protein A was utilized to detect lymphocyte binding of IgG at 37 degrees C in 50 sera from patients with SLE. IgG binding was partially inhibited by preincubation of test cells with immune complexes in 50% of sera. In 10%, cytophilic Ig was increased by immune complex preincubation, and in 40% no appreciable change was recorded. With purified preparations of T and B cells, binding of SLE IgG at 37 degrees C was equal for both cell populations.
D001402:B-Lymphocytes / Q000378:metabolism; D001426:Bacterial Proteins / Q000276:immunology*; D004195:Disease Models, Animal; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D013211:Staphylococcus aureus; D013601:T-Lymphocytes / Q000378:metabolism
D001426:Bacterial Proteins; D007074:Immunoglobulin G