title: The nature of the deoxyribonucleosides involved in the binding of carcinogenic hydrocarbons to the DNA of mouse embryo cells.
abstract: The DNA of mouse embryo cells was specifically labelled in the purine moieties with (G(3)H)-deoxyadenosine or in the cytosine moieties with (5-(3)H)-deoxycytidine. These cells were then treated with 7-methylbenz (a) anthracene (7MBA) or benzo (a)-pyrene (B(a)P) and the DNA isolated, degraded and fractionated by LH20 Sephadex column chromatography. When the purines of the DNA were tritium-labelled, radioactive hydroccarbondeoxyribonucleoside products were obtained. No such products were found with deoxycytidine pre-labelled DNA. Contrary to an earlier suggestion, these results indicate that it is the purine moieties of DNA which react with the metabolically activated hydrocarbon derivative in vivo.
mesh_terms: D000818:Animals; D001551:Benz(a)Anthracenes / Q000378:metabolism; D001580:Benzopyrenes / Q000378:metabolism; D001665:Binding Sites; D002273:Carcinogens / Q000378:metabolism*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D003839:Deoxyadenosines / Q000378:metabolism; D003841:Deoxycytidine / Q000494:pharmacology; D003853:Deoxyribonucleosides / Q000378:metabolism*; D004622:Embryo, Mammalian; D006838:Hydrocarbons / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011687:Purines / Q000378:metabolism
chemical_list: D001551:Benz(a)Anthracenes; D001580:Benzopyrenes; D002273:Carcinogens; D003839:Deoxyadenosines; D003853:Deoxyribonucleosides; D006838:Hydrocarbons; D011687:Purines; D003841:Deoxycytidine; D004247:DNA