Identification of multiple cytolytic components associated with the beta-LT class of lymphotoxins released by mitogen-activity human lymphocytes in vitro.
Cytotoxic activity (lymphotoxin (LT) associated with the 50,000 dalton beta-LT class of human lymphotoxins has been resolved into multiple components by chromatography on DEAE-cellulose or electrophoresis on polyacrylamide gels. The two components, termed beta-LT, and beta-LT2, are separable from the 90,000 m.w. alpha-LT, since neither component is neutralized by incubation with heterologous rabbit anti-alpha-LT antisera. However, the two components differ in their overall charge and relative heat stability with the betaLT1 component being more positively charged than the beat-LT2 molecule and extremely heat labile.
D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008233:Lymphotoxin-alpha / Q000032:analysis*; D008970:Molecular Weight
D008233:Lymphotoxin-alpha