High resolution scanning electron microscopy at the subcellular level.
Recently developed scanning electron microscopes provide sufficient resolution to allow useful observation of subcellular biological objects. Preparation methods for such objects need not be limited to the traditional coating and mounting procedures. Many methods developed for transmission electron microscopy are immediately adaptable to scanning electron microscopy. We show that a number of techniques are available to the microscopist which yield adequate contrast and high resolution. As examples we show skeletal muscle myofibrils dispersed to reveal thick filaments, uncoated on a thin carbon film; a tropomyosin tactoid, negatively stained with uranyl acetate; oncornavirus, conventionally coated; and T4 bacteriophage on an aluminium substrate.
D000818:Animals; D002460:Cell Line; D003090:Coliphages / Q000648:ultrastructure; D004267:DNA Viruses; D006652:Histological Techniques; D007668:Kidney; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009132:Muscles / Q000648:ultrastructure; D009210:Myofibrils / Q000648:ultrastructure; D009858:Oncogenic Viruses / Q000648:ultrastructure; D011817:Rabbits; D051381:Rats; D013347:Subcellular Fractions / Q000648:ultrastructure*; D014335:Tropomyosin
D014335:Tropomyosin