Lipid A, the active part of bacterial endotoxins in inducing serum colony stimulating activity and proliferation of splenic granulocyte/macrophage progenitor cells.
An analysis of which component of lipopolysaccharides (LPS), the lipid or the polysaccharide (PS), is active in stimulating the murine granulopoietic system has been performed. LPS with different structures, isolated from different mutant strains of Salmonella and chemical degradation products of lipopolysaccharides have been used. Lipid A obtained by acid hydrolysys of the LPS and complexed to bovine serum albumin (BSA) (lipid A-BSA) was shown to be active in generating serum colony stimulating factor (CSF) and in increasing the splenic colony forming cells (CFC) levels, although it was less active than the parent LPS. The polysaccharide (PS) showed no significant activity at the concentrations used. LPS (glycolipids) from R mutants of Salmonella minnesota were active to the same extent as the LPS. The fact that even the most defective LPS from the R mutant R595 which contains lipid A and KDO only is a potent endotoxin, points unequivocally, to lipid A, as the active principle in stimulating the granulopoietic system.
D000818:Animals; D002999:Clone Cells / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000494:pharmacology*; D006017:Glycolipids / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis*; D006098:Granulocytes / Q000187:drug effects*; D006410:Hematopoiesis / Q000187:drug effects*; D007962:Leukocytes / Q000187:drug effects*; D008055:Lipids / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D012475:Salmonella
D003115:Colony-Stimulating Factors; D004731:Endotoxins; D006017:Glycolipids; D006023:Glycoproteins; D008055:Lipids; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial