A glycoprotein associated with the membrane fraction of human B but not T lymphocytes.
A method is described which employs differential centrifugation and sucrose density gradient centrifugation to isolate a membrane fraction from human lymphocytes. Membrane preparations from long-term human cultured B- and T-lymphoid lines, peripheral blood lymphocytes, tonsillar lymphocytes, and thymocytes were analyzed on 0.5% sodium dodecyl sulfate-7.5% polyacrylamide gels stained for protein and carbohydrate. The most important finding was a major glycoprotein of approximately 30,000 daltons associated with the membrane preparations from B lymphocytes. T-lymphocyte preparations did not contain readily detectable amounts of this membrane-associated component. The T-cell lymphoid line MOLT-4 was unique in that it had a narrow protein band at approximately 30,000 daltons which did not contain carbohydrate.
D000941:Antigens / Q000032:analysis; D001402:B-Lymphocytes / Q000032:analysis* / Q000648:ultrastructure; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007945:Leukemia, Lymphoid / Q000097:blood; D014066:Palatine Tonsil / Q000166:cytology; D012967:Sodium Dodecyl Sulfate; D013601:T-Lymphocytes / Q000032:analysis* / Q000648:ultrastructure; D013950:Thymus Gland / Q000166:cytology
D000941:Antigens; D006023:Glycoproteins; D007136:Immunoglobulins; D012967:Sodium Dodecyl Sulfate