title: [Purification of microbial asparaginases by the aid of affinity chromatography].
abstract: Asparaginases from Escherichia coli and Erwinia caratovora were isolated and purified by column chromatography with a specific sorbent (sepharose, covalently bound to N-alpha-(6-aminohexyl)-D-asparagine). Homogenous asparaginase from E. coli was isolated by one-step procedure, while the enzyme from Er. carotovara was 50-60-fold purified. Asparaginase from Mycobacterium n. sp. was found not to bind with the specific sorbent.
mesh_terms: D001215:Asparaginase / Q000302:isolation & purification*; D001216:Asparagine / Q000031:analogs & derivatives / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods*; D004590:Electrophoresis, Paper; D004591:Electrophoresis, Polyacrylamide Gel; D004885:Erwinia / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology*; D006868:Hydrolysis; D007202:Indicators and Reagents; D009161:Mycobacterium / Q000201:enzymology; D012685:Sepharose / Q000031:analogs & derivatives; D013050:Spectrometry, Fluorescence
chemical_list: D007202:Indicators and Reagents; D001216:Asparagine; D012685:Sepharose; D001215:Asparaginase
vernacular_title: Ochistka mikrobnykh asparaginaz s pomoshch'iu khromatografii po srodstvu