Steffimycin B, a DNA binding agent.
The antibiotic steffimycin B binds to double-stranded DNA as evidenced by difference spectroscopy and an increase of the thermal stability of DNA in the presence of the antibiotic. Salmon sperm DNA-steffimycin B complexes show a drastic decrease in template activity for Escherichia coli DNA polymerase I but not for DNA-idrected RNA polymerase. The differences in template properties of poly[d(A-T)] and poly (dG) - poly(dC)-antibiotic complexes,respectively, for DNA polymerase I and RNA polymerase suggest that the antibiotic interacts primarily with adenine or thymine bases or both in double-stranded DNA.
D000818:Animals; D018943:Anthracyclines; D000880:Anthraquinones / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004355:Drug Stability; D004926:Escherichia coli / Q000201:enzymology; D006027:Glycosides / Q000378:metabolism; D008297:Male; D011119:Polynucleotides / Q000378:metabolism; D012474:Salmon; D013094:Spermatozoa; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects
D018943:Anthracyclines; D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D006027:Glycosides; D011119:Polynucleotides; C009944:steffimycin; D004247:DNA; D004254:DNA Nucleotidyltransferases; D012321:DNA-Directed RNA Polymerases