Effect of UV irradiation on transduction by coliphage T1.
Lysates of the virulent coliphage T1 transduce seven markers between strains of Escherichia coli with reproducilble efficiencies which range from 10(-7) to 10(-5). The ability of a UV-irradiated lysate to transduce Arg(+), Str(R), Trp(+), Lac(+), and Pro(+) is 90% and Bio(+) is 99% inactivated by doses which inactivate plaque formation of T1 by six orders of magnitude. A dose of irradiation which causes a 1- to 2-log drop in the titer of T1 stimulated Gal(+) transduction by two- to three-fold; no other marker tested was stimulated. Irradiation causes dislinkage of some co-transducible markers but not others.
D001120:Arginine / Q000096:biosynthesis; D001710:Biotin / Q000096:biosynthesis; D003090:Coliphages / Q000254:growth & development; D003545:Cysteine / Q000096:biosynthesis; D004267:DNA Viruses; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D008040:Genetic Linkage / Q000528:radiation effects; D007785:Lactose / Q000378:metabolism; D008242:Lysogeny; D009154:Mutation; D011392:Proline / Q000096:biosynthesis; D011830:Radiation Effects; D013307:Streptomycin / Q000494:pharmacology; D014161:Transduction, Genetic / Q000528:radiation effects*; D014364:Tryptophan / Q000096:biosynthesis; D014466:Ultraviolet Rays
D001710:Biotin; D014364:Tryptophan; D001120:Arginine; D011392:Proline; D007785:Lactose; D003545:Cysteine; D005690:Galactose; D013307:Streptomycin