Cell-mediated allograft responses in vitro. VI. Studies on macrophage-mediated cytotoxicity.
Normal murine peritoneal macrophages were rendered cytotoxic against 51Cr-labelled allogeneic and syngeneic target cells by incubation with supernatant of selected cell cultures.'Active' culture supernatant was produced both by specifically sensitized cytotoxic T lymphocytes as well as by mitogen-stimulated T cells, but not by mitogen-stimulated B cells. The in vitro induced macrophage-mediated cytotoxicity was found to be non-specific in the sense that 51Cr-labelled target cells of different H-2 haplotype were lysed equally well.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D046508:Culture Techniques; D003601:Cytotoxicity Tests, Immunologic; D006238:Haploidy; D006648:Histocompatibility; D007111:Immunity, Cellular; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D037102:Lectins; D008070:Lipopolysaccharides