The influence of protease inhibitor on hydroproteolytic activity and serotonin content in the intestine of rats after fractionated irradiation.
Hydroproteolytic activity and content of serotonin in the small intestine and colon were studied in rats X-irradiated on the whole body with fractionated doses of 5 X 150 R. Twenty-four hours after exposure to X-rays, lipase, acid phosphatase activities and cathepsin activity as well as serotonin content were assayed in the intestinal tissues. Irradiation altered the hydroproteolytic activity of the tissues, in a majority of cases by increasing hydrolytic activity and lowering proteolytic (catheptic) activity in all parts of the small intestine and decreased serotonin content in the first and third segments of the small intestine. Trasylol injected s.c. in doses of 10,000 units/kg before and after irradiation modified hydrolytic tissue activity of the studied segments of the intestine and lessened the fall in serotonin content of the intestines after irradiation.
D000135:Acid Phosphatase / Q000378:metabolism / Q000528:radiation effects; D000818:Animals; D007611:Aprotinin / Q000494:pharmacology*; D002403:Cathepsins / Q000378:metabolism / Q000528:radiation effects; D007421:Intestine, Small / Q000032:analysis / Q000528:radiation effects*; D008049:Lipase / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D011829:Radiation Dosage; D011830:Radiation Effects; D011833:Radiation Injuries, Experimental / Q000201:enzymology*; D051381:Rats; D012701:Serotonin / Q000032:analysis*
D012701:Serotonin; D007611:Aprotinin; D008049:Lipase; D000135:Acid Phosphatase; D002403:Cathepsins