Isolation of a nontransmissible antibiotic resistance plasmid by transductional shortening of R factor RP1.
A plasmid segregant carrying tetracycline and carbenicillin resistance markers has been isolated from R factor RP1 by transductional shortening with phage P22. The new plasmid RP1-S2, which has a molecular weight of 23 times 10-6, has lost the transfer, phage sensitivity, and neomycin resistance functions of RP1. It combines readily with a W group plasmid, R388, to form a transmissible carbenicillin and trimethoprim resistance plasmid, RWP1.
D002228:Carbenicillin / Q000494:pharmacology; D003090:Coliphages / Q000378:metabolism; D004269:DNA, Bacterial / Q000302:isolation & purification* / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005111:Extrachromosomal Inheritance; D010403:Penicillin Resistance; D010957:Plasmids; D011815:R Factors; D012483:Salmonella Phages / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects / Q000378:metabolism; D013752:Tetracycline / Q000494:pharmacology; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D013752:Tetracycline; D002228:Carbenicillin