The biological activity of bacteriophage DNA, prepared by the cationic detergent dilution technique.
The preparation of phage lambda DNA infecting E. coli K 12 with cationic detergent is described. This DNA infects E. coli spheroblasts with the same efficiency as DNA prepared by phenol methods.
D002852:Chromatography, Ion Exchange; D003090:Coliphages / Q000378:metabolism*; D004267:DNA Viruses / Q000378:metabolism; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism*; D003902:Detergents; D004926:Escherichia coli / Q000378:metabolism; D000644:Quaternary Ammonium Compounds; D010948:Viral Plaque Assay; D014779:Virus Replication
D004279:DNA, Viral; D003902:Detergents; D000644:Quaternary Ammonium Compounds