Rosette formation of pig T lymphocytes with sheep erythrocytes.
The relationship of sheep RBC rosette formation to density of thymus and blood lymphocytes was investigated. Thymocyte density was unimodal and cells of all densities rosetted equally. Blood lymphocyte density was bimodal with most rosette-forming cells in the denser ficoll layers. Papain treatment of SRBC increases rosette formation with blood lymphocytes while apparently maintaining specificity of T cells.
D000818:Animals; D000918:Antibody Specificity; D002417:Cattle / Q000276:immunology; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D005362:Ficoll; D007104:Immune Adherence Reaction; D009439:Neuraminidase / Q000494:pharmacology; D010206:Papain / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012756:Sheep / Q000276:immunology; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
D011947:Receptors, Antigen, B-Cell; D005362:Ficoll; D009439:Neuraminidase; D010206:Papain