title: Induction of cell-mediated immunity in the mouse: circumstantial evidence for highly immunogenic antigen in the regional lymph nodes following skin painting with contact sensitizing agents.
abstract: This paper describes an investigation of why contact sensitizing agents cause strong cell-mediated immunity. Contact sensitivity was induced in mice by painting the skin with 4-ethoxymethylene-2-phenyloxazolone (oxazolone), and measured by the increase of ear thickness following challenge six days later. Reactivity was transferred by taking the regional lymph node cells from mice 18 h after immunization and injecting them into the footpads of recipients. This "18-h transfer" has several characteristics. As few as 2 X 10(4) cells were effective. The donor lymph node cells were best taken one to three days after immunization, were less effective on day 4 and virtually inactive by day 7. The recipients developed contact sensitivity when challenged on day 4, but lacked sensitivity when challenged on days 1 and 2 after transfer. The transferred cells were still active after treatment with anti-theta serum and complement. They also resisted 2,000 R in vitro, mitomycin, vinblastine, and inhibitors of protein synthesis such as emetine, cycloheximide and puromycin. The transfer was prevented by treatment with trypsin, freeze-thawing, and heating at 56 C. Plasma membranes were also immunogenic. The evidence suggests that the "18-h transfer" is a special type of active immunization, not due to ordinary free oxazolone, and that the agent is present within the lymph node in a free oxazolone, and that the agent is present within the lymph node in a specially immunogenic location or form.
mesh_terms: D000818:Animals; D000941:Antigens; D000961:Antilymphocyte Serum; D002462:Cell Membrane / Q000276:immunology; D003165:Complement System Proteins; D003877:Dermatitis, Contact / Q000276:immunology*; D005615:Freezing; D006358:Hot Temperature; D007111:Immunity, Cellular; D007116:Immunization, Passive; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D010080:Oxazoles / Q000494:pharmacology*; D010081:Oxazolone / Q000494:pharmacology*; D012867:Skin / Q000276:immunology; D013601:T-Lymphocytes; D013997:Time Factors; D014357:Trypsin
chemical_list: D000941:Antigens; D000961:Antilymphocyte Serum; D010080:Oxazoles; D010081:Oxazolone; D003165:Complement System Proteins; D014357:Trypsin