Rejection of tumor cells in vitro: a T-cell-mediated reaction.
Cytoxic lymphoblasts (LBC) were produced by the co-cultivation of guinea-pig lymphoid cells from the thymus, lymph nodes, spleen, bone marrow and blood with syngeneic methylcholanthrene-induced sarcoma (MC-D) cells. The cytotoxic reaction was inhibited by pretreatment of the lymphoid cell populations with a rabbit antiserum specific for guinea-pig thymus-derived (T) lymphocytes. Supernatants of LBC inhibited the migration of peritoneal exudate cells, but did not contain lymphotoxin or a skin reactive factor. It is concluded that the cytotoxic cells are of T-cell origin.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000961:Antilymphocyte Serum; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D006168:Guinea Pigs; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology; D009368:Neoplasm Transplantation; D011817:Rabbits / Q000276:immunology; D012513:Sarcoma, Experimental / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014185:Transplantation, Isogeneic
D000961:Antilymphocyte Serum