[Determination of the optical constants of intact microogranisms by the method of attenuated internal reflection spectroscopy].
The problems are considered of the determination of the optical constants (dispersion of refraction, n2, and absorption, K, indexes) of strongly dispersing biological objects on undestroyed cells of microorganisms (E. coli, strain C-85) by the method of repeated attenuated total refraction. The volume of unpacked intercellular space and the effect of repeated propagation of the light current through measuring elements with high refraction index were taken into account. Relationships R, s (n1, n2, O) K were obtained for determining the optical constants on the elements with the angle of light current incidence 0=45 degrees, made of infrared glass, IRG-24, IRF-24 and of Si and Ge as well. The optical constants of bacteria in the region of absorption bands 1660, 1550 cm-1 were also determined.
D000190:Actinomyces / Q000032:analysis; D001419:Bacteria / Q000032:analysis*; D002175:Candida / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis; D005658:Fungi / Q000032:analysis*; D013057:Spectrum Analysis / Q000379:methods
Osobennosti opredeleniia opticheskikh postoiannykh nerazrushennykh mikroorganizmov metodom spektroskopii NPVO