Transmissible substrate-utilizing ability in enterobacteria.
Three of 152 strains of Escherichia coli transmitted their ability to utilize sucrose (Sac+) to other strains by conjugation. The transfer factor of one of them and of a Sac+ Salmonella thompson strain was thermosensitive. The raffinose-utilizing ability of 27 of 163 E. coli strains was also transmissible. Transmissible raffinose-utilizing ability was a feature of porcine enterpathogenic strains possessing the K88 antigen. The determinants controlling raffinose utilization (Raf) and K88 antigen production were commonly transmitted together from these strains; so also was the determinant controlling enterotoxin production, but to a lesser extent. It was not possible to transfer lactose-utilizing ability from 320 strains of E. coli, salicin-utilizing ability from 12 strains of E. coli or dulcitol-utilizing ability from 99 strains of E. coli and 88 strains of salmonellae. Sucrose- and raffinose-utilizing ability were transmitted separately to several Salmonella sp., including Salm. typhi, to Shigella flexneri and Sh. sonnei and to a variety of strains of E. coli. A strain of Salm. typhimurium in which Sac had been established and a strain of E. coli in which Raf had been established survived less well in the alimentary tract of chickens than their Sac minus or Raf minus parent strains.
D000818:Animals; D050260:Carbohydrate Metabolism; D002645:Chickens; D003227:Conjugation, Genetic; D004064:Digestive System / Q000382:microbiology; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000378:metabolism*; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000302:isolation & purification / Q000378:metabolism; D005111:Extrachromosomal Inheritance; D005243:Feces / Q000382:microbiology; D004376:Galactitol / Q000378:metabolism; D005960:Glucosides / Q000378:metabolism; D006460:Hemolysin Proteins / Q000096:biosynthesis; D007785:Lactose / Q000378:metabolism; D011887:Raffinose / Q000378:metabolism; D012475:Salmonella / Q000378:metabolism; D012486:Salmonella typhimurium / Q000302:isolation & purification; D012760:Shigella / Q000378:metabolism; D013045:Species Specificity; D013395:Sucrose / Q000378:metabolism; D013696:Temperature; D013752:Tetracycline / Q000494:pharmacology
D004768:Enterotoxins; D005960:Glucosides; D006460:Hemolysin Proteins; D004376:Galactitol; D013395:Sucrose; D013752:Tetracycline; D007785:Lactose; D011887:Raffinose