Topology of binding sites for carbamyl phosphate in aspartate transcarbamylase from Escherichia coli. The use of pyridoxal phosphate as covalent probe.
Pyridoxal phosphate, a competitive inhibitor of aspartate transcarbamylase, binds to six sites in the catalytic and to twelve sites in the regulatory subunits of this hexameric protein. The properties of its association to the active sites of the enzyme are very similar to those observed with one of its substrates, carbamyl phosphate. It tightly binds to one half of the sites in the absence of succinate, an analogue of the second substrate. Since pyridoxal phosphate can be linked covalently to the protein by reduction, the distribution of the high affinity binding sites on catalytic trimers was studied after dissociation of modified holoenzyme. Electrophoresis of isolated subunits under non-denaturing conditions revealed four distinct bands, corresponding to trimers containing 0 to 3 pyridoxal phosphate derivatives. The distribution among the four species as a function of ligand concentration in the absence of succinate indicates that in the native oligomer, pyridoxal phosphate (and by extrapolation, carbamyl phosphate) binds to both catalytic trimers, rather than to three sites on a single subunit.
D001221:Aspartate Carbamoyltransferase / Q000037:antagonists & inhibitors; D001665:Binding Sites; D001667:Binding, Competitive; D001894:Borohydrides; D002219:Carbamates; D002221:Carbamyl Phosphate; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D007553:Isotope Labeling; D046911:Macromolecular Substances; D010084:Oxidation-Reduction; D011485:Protein Binding; D011732:Pyridoxal Phosphate / Q000138:chemical synthesis; D013386:Succinates
D001894:Borohydrides; D002219:Carbamates; D046911:Macromolecular Substances; D013386:Succinates; D002221:Carbamyl Phosphate; D011732:Pyridoxal Phosphate; D001221:Aspartate Carbamoyltransferase