title: The role of plant particles, bacteria and cell-free supernatant fractions of rumen contents in the hydrolysis of trilinolein and the subsequent hydrogenation of linoleic acid.
abstract: The role of different fractions of rumen contents in the hydrolysis of trilinolein and the subsequent hydrogenation of the linoleic acid has been investigated by a series of in vitro incubations. Hydrolysis of the trilinolein to free linoleic acid occurs almost wholly in the cell-free supernatant; the liberated linoleic acid in the supernatant can be rapidly adsorbed onto food particles where it is hydrogenated to stearic acid via the C18 trans-11 monoene. Some 25% of the trilinolein added as substrate was taken up by the bacteria and of this a small percentage appeared to be hydrolysed and the free linoleic acid hydrogenated to stearic acid intracellularly. No conclusive evidence was obtained to suggest that this intracellular hydrogenation proceeded by a route other than that which took place on the food particles.
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002474:Cell-Free System; D005227:Fatty Acids / Q000032:analysis; D006865:Hydrogenation; D006868:Hydrolysis; D008041:Linoleic Acids / Q000378:metabolism*; D008055:Lipids / Q000032:analysis; D010944:Plants / Q000378:metabolism; D012417:Rumen / Q000378:metabolism* / Q000382:microbiology; D012756:Sheep; D013229:Stearic Acids / Q000378:metabolism; D013347:Subcellular Fractions
chemical_list: D005227:Fatty Acids; D008041:Linoleic Acids; D008055:Lipids; D013229:Stearic Acids