Mouse B and T lymphocyte responses to purified timothy pollen antigens in vitro.
Purified populations of splenic B and T lymphocytes from LAF mice immunized with a crude extract of timothy pollen (WST) responded specifically to pollen antigens in an in vitro lymphocyte transformation system. The peak lymphocyte transformation response occurred 5 days after a secondary immunization and was the result of T-B cell cooperation in vitro. Wtih two purified pollen antigens as in vitro stimulants we were able to define at least two antigen-specific population of B cells and one population of T cells. These results were confirmed by inhibition studies with a monovalent hapten from WST, Antigen D.
D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D001667:Binding, Competitive; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D010936:Plant Extracts / Q000276:immunology; D006109:Poaceae / Q000276:immunology*; D011058:Pollen; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000936:Antigen-Antibody Complex; D000941:Antigens; D010936:Plant Extracts