Biological relevance of gamma-ray-induced alkali-labile sites in single-stranded Dna in aqueous solutions.
Gamma-irradiation in single-stranded phiX174 DNA in aqueous solution in the presence of oxygen produces at least two types of alkali-labile site. One is lethal and gives rise to single-strand breaks by treatment with alkali. The other is non-lethal, but is converted to lethal damage by alkali. The effect of alkali is dependent on temperature. This dependence is different for both types of alkali-labile site.
D003090:Coliphages; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004279:DNA, Viral / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D010100:Oxygen; D012972:Sodium Hydroxide; D012996:Solutions; D013696:Temperature; D014867:Water
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012996:Solutions; D014867:Water; D012972:Sodium Hydroxide; D010100:Oxygen