Lipid synthesis in stringent Escherichia coli: an artifact in acetate labeling of phospholipids during a shiftdown in growth rate.
The use of (14C)acetate to label the phospholipids of stringent Escherichia coli, after a decrease in agitation, leads to artifacts resulting from a decrease in the specific activity of the acetyl coenzyme A pool.
D000085:Acetates / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000378:metabolism; D001120:Arginine / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005796:Genes; D005947:Glucose / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008239:Lysine / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis*; D010761:Phosphorus Radioisotopes; D012329:RNA, Bacterial / Q000096:biosynthesis; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D000085:Acetates; D001426:Bacterial Proteins; D008055:Lipids; D010710:Phosphates; D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D012329:RNA, Bacterial; D014316:Tritium; D014498:Uracil; D000105:Acetyl Coenzyme A; D001120:Arginine; D005947:Glucose; D008239:Lysine