Fractionation of cytotoxic cells from tumour-immune rats on derivatized collagen gels.
Incubation at 37 degrees C of spleen cells from rats, immunized with Gross virus-induced lymphoma, on collagen gels coated with membrane extracts of lymphoma cells yielded two different cell populations. The non-adherent cells showed greatly diminished cytotocicity for lymphoma cells compared with unfractionated spleen cell suspensions, whilst the specifically adherent cells which were recovered after collagenase digestion of the gels, showed enrichment of cytotoxic activity.
D000818:Animals; D000951:Antigens, Neoplasm; D002469:Cell Separation / Q000379:methods*; D003094:Collagen; D005260:Female; D005782:Gels; D007114:Immunization; D008223:Lymphoma / Q000276:immunology*; D008297:Male; D051381:Rats; D011920:Rats, Inbred WF; D013601:T-Lymphocytes / Q000276:immunology
D000951:Antigens, Neoplasm; D005782:Gels; D003094:Collagen