title: Uptake of adenosine 5'-monophosphate by Escherichia coli.
abstract: Adenosine 5'-monophosphate is dephosphorylated before its uptake by cells of Escherichia coli. This is demonstrated by using a radioactive double-labeled culture, and with a 5'-nucleotidase-deficient, mutant strain. The adenosine formed is further phosphorolyzed to adenine as a prerequisite for its uptake and incorporation. The cellular localization of the enzymes involved in the catabolism of adenosine 5'-monophosphate is discussed.
mesh_terms: D000225:Adenine / Q000378:metabolism; D000241:Adenosine / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D008851:Micropore Filters; D008954:Models, Biological; D009154:Mutation; D009696:Nucleic Acids / Q000096:biosynthesis; D009708:Nucleotidases / Q000096:biosynthesis; D010430:Pentosyltransferases / Q000096:biosynthesis; D010761:Phosphorus Radioisotopes; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D009696:Nucleic Acids; D010761:Phosphorus Radioisotopes; D014316:Tritium; D000249:Adenosine Monophosphate; D010430:Pentosyltransferases; D009708:Nucleotidases; D000225:Adenine; D000241:Adenosine