Activation of T-lymphocyte helper function by brief exposure to antigen-pulsed macrophages.
In this report we investigated the ability of macrophage-associated OVA to stimulate purified OVA-immune T-lymphocytes to produce nonspecific helper activity for antibody production. T-cell activation required only a brief contact between the antigen-pulsed macrophages and T-lymphocytes and was maximal after a 4-hr contact. T-cell activation occurred at 37 but not at 4 degrees C, indicating that the macrophages and/or lymphocytes must be metabolically active for efficient activation. In addition, we compared the ability of antigen-pulsed syngeneic or allogeneic macrophages to activate T-cells for helper function.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D006649:Histocompatibility Antigens; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013696:Temperature
D000941:Antigens; D006649:Histocompatibility Antigens; D010047:Ovalbumin