Transductional mapping of gene trmA responsible for the production of 5-methyluridine in transfer ribonucleic acid of Escherichia coli.
The gene trmA, responsible for the production of 5-methyluridine (ribothymidine) in transfer ribonucleic acid, has been located at 79 min on the chromosomal map of Escherichia coli K-12. In five-factor crosses the gene order was shown to be argH-trmA-rif-thiA-metA. The co-transduction frequency between argH and trmA was 65%. Furthermore, the trmA5 mutation was shown to be recessive, in agreement with the notion that the trmA gene is the structural gene for the transfer tibonucleic acid (5-methyluridine) methyltransferase.
D002874:Chromosome Mapping; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005796:Genes; D005808:Genes, Recessive; D008745:Methylation; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000096:biosynthesis*; D014161:Transduction, Genetic; D014529:Uridine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D012359:tRNA Methyltransferases / Q000096:biosynthesis
D012329:RNA, Bacterial; D012343:RNA, Transfer; D012359:tRNA Methyltransferases; D014529:Uridine