Interaction of 6,6'-dithiodinicotinic acid with rat thymocytes pretreated with thiol compounds.
The number of sulfhydryl groups in the membranes of rat thymocytes were determined, expressed in terms of concentration of 6-mercaptonicotinic acid produced by reduction of 6,6'-dithiodinicotinic acid. The influence of incubation of thymocytes in solutions of L-cysteine, reduced glutathione and dithiothreitol on the number of -SH groups was studied. About twofold higher degree of reduction of 6-6'dithiodinicotinic acid was observed in the reaction with thymocytes incubated previously in 2 mM solution of L-cysteine in comparison with controls. Incubation of thymocytes with dithiothreitol and gluthathione had no effect on the number of -SH groups.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D004220:Disulfides / Q000494:pharmacology; D004347:Drug Interactions; D005260:Female; D005978:Glutathione / Q000378:metabolism; D009539:Nicotinic Acids / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D004220:Disulfides; D009539:Nicotinic Acids; D013438:Sulfhydryl Compounds; D005978:Glutathione; D003545:Cysteine