Maltose chemoreceptor of Escherichia coli.
Strains carrying mutations in the maltose system of Escherichia coli were assayed for maltose taxis, maltose uptake at 1 and 10 muM maltose, and maltose-binding activity released by osmotic shock. An earlier conclusion that the metabolism of maltose is not necessary for chemoreception is extended to include the functioning of maltodextrin phosphorylase, the product of malP, and the genetic control of the maltose receptor by the product of malT is confirmed. Mutants in malF and malK are defective in maltose transport at low concentrations as well as high concentrations, as previously shown, but are essentially normal in maltose taxis. The product of malE has been previously shown to be the maltose-binding protein and was implicated in maltose transport. Most malE mutants are defective in maltose taxis, and all those tested are defective in maltose transport at low concentrations. Thus, as previously suggested, the maltose-binding protein probably serves as the recognition component of the maltose receptor, as well as a component of the transport system. tsome malE mutants release maltose-binding activity and are tactic toward maltose, although defective in maltose transport, implying that the binding protein has separate sites for interaction with the chemotaxis and transport systems. Some mutations in lamB, whose product is the receptor for the bacteriophage lamba, cause defects in maltose taxis, indicating some involvement of that product in maltose reception.
D001224:Aspartic Acid / Q000494:pharmacology; D001693:Biological Transport, Active; D002250:Carbon Radioisotopes; D002633:Chemotaxis; D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D005690:Galactose / Q000494:pharmacology; D005796:Genes; D008320:Maltose / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation; D009876:Operon; D006005:Phosphorylases / Q000096:biosynthesis; D011485:Protein Binding
D002250:Carbon Radioisotopes; D001224:Aspartic Acid; D008320:Maltose; D006005:Phosphorylases; D005690:Galactose