Fixation and loss of hydrazine-induced premutational damage in Haemophilus influenzae.
Premutational damage induced in Haemophilus influenzae by hydrazine appears to be fixed as final mutation only at replication as judged by the transformation assay. Fixation at replication is independent of the rec1 gene, unlike the case with nitrosocarbaryl. Prior to replication premutational damage induced by hydrazine disappears by an unknown process that is not dependent on the presence of a pyrimidine dimer excision system nor on the rec1 gene. Hydrazine does not produce detectable single-strand breaks or alkali-labile sites in the treated DNA nor gaps in DNA newly synthesized after treatment. In these respects it also differs from nitroso compounds. It is concluded that hydrazine acts to produce and altered base, possibly N(4)-aminocytosine, that produces mutations by mispairing at replication rather than by error-prone repair.
D002876:Chromosomes, Bacterial / Q000187:drug effects; D004261:DNA Replication; D005796:Genes; D006193:Haemophilus influenzae / Q000187:drug effects*; D006834:Hydrazines / Q000494:pharmacology*; D009154:Mutation; D011743:Pyrimidines / Q000378:metabolism
D006834:Hydrazines; D011743:Pyrimidines