Reversibility of the affinity labelled-biotin transport system in yeast cells.
Transport of biotin by Saccharomyces cerevisiae is inhibited by biotynyl p-nitrophenyl ester. Conversion of the inhibited cells to spheroplasts or simple treatment with thiols results in a total restoration of vitamin transport. Biotynyl p-nitrophenyl ester-induced inhibition is not due to an intracellular accumulation of the vitamin and consequent regulation, but appears to be due to specific labelling  of the transport system.
D001692:Biological Transport / Q000187:drug effects; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism*; D003864:Depression, Chemical; D009596:Nitrophenols / Q000037:antagonists & inhibitors / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000494:pharmacology
D009596:Nitrophenols; D013438:Sulfhydryl Compounds; D001710:Biotin