title: A macromolecule-free partially defined medium for Trypanosoma cruzi.
abstract: A macromolecule-free medium, containing in its defined part 3 salts, glucose, hemin, 21 amino acids, 3 lipids, and some undefined components obtained by dialysis of liver infusion, was developed for serial cultivation of Trypanosoma cruzi at 28 C.  The medium allows prolonged cultivation of T. cruzi by serial transfers and growth comparable to that obtained in more complex media, including those containing blood serum.
mesh_terms: D000596:Amino Acids; D002784:Cholesterol; D003470:Culture Media; D003956:Dialysis; D005612:Freeze Drying; D005947:Glucose; D006454:Hemoglobins; D008110:Liver Extracts; D046911:Macromolecular Substances; D010710:Phosphates; D011189:Potassium Chloride; D012964:Sodium; D012965:Sodium Chloride; D013229:Stearic Acids; D014349:Trypanosoma cruzi / Q000254:growth & development*
chemical_list: D000596:Amino Acids; D003470:Culture Media; D006454:Hemoglobins; D008110:Liver Extracts; D046911:Macromolecular Substances; D010710:Phosphates; D013229:Stearic Acids; D012965:Sodium Chloride; D011189:Potassium Chloride; D002784:Cholesterol; D012964:Sodium; D005947:Glucose