Number of genes and base composition of mitochondrial tRNA from Saccharomyces cerevisiae.
Increasing amounts of mitochondrial [32P] tRNA (4S fraction), were hybridized with mitochondrial DNA OF Saccharomyces cerevisiae. At saturation, the calculated number of genes for 4S mitochondrial RNA was 20. Mitochondrial [32P] tRNA eluted from the hydrids obtained either with an excess of tRNA or an excess of DNA showed, after alkaline hydrolysis and chromatography, a G+C content of 28 and 35 p. cent respectively. This last value is similar to that found with the total 4S fraction. The odd nucleotides T (about 1T per sequence), U, hU are present in mitochondrial tRNA. Some sequence may begin with pG.
D001345:Autoradiography; D004272:DNA, Mitochondrial; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D008928:Mitochondria / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012343:RNA, Transfer; D012265:Ribonucleotides / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D004272:DNA, Mitochondrial; D012265:Ribonucleotides; D012343:RNA, Transfer