Changes in agglutination of cells with H-2 antibodies or lectins and in stimulation of lymphocytes as induced by certain polymers (chondroitin sulphate/PVP/PHA/Con A/capping of H-2 antigens).
The effect of certain polymers on the course of immune interaction has been well known although its mechanism at the cellular level remains obscure. We thus affected cells in vitro with two of such substances, CS and PVP, and followed their behaviour in various assays. It was shown that in this way the agglutinability of mouse erythrocytes with H-2 antibodies or of mouse lymphocytes with threshold doses of Con A could be enhanced, antibody-induced capping of H-2 antigens on lymphocytes accelerated and PHA-induced stimulation of mouse lymphocytes inhibited. The possible mechanisms of these changes putatively mediated through the effect on the cell membrane or cell coat are discussed.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology*; D006025:Glycosaminoglycans / Q000494:pharmacology; D006384:Hemagglutination; D006386:Hemagglutination Tests; D006649:Histocompatibility Antigens; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D011205:Povidone / Q000494:pharmacology*
D006025:Glycosaminoglycans; D006649:Histocompatibility Antigens; D003208:Concanavalin A; D002807:Chondroitin; D002809:Chondroitin Sulfates; D011205:Povidone