A specific radio-immunoassay for gonadotrophin-releasing hormone.
A specific antiserum has been made to synthetic gonadotrophin-releasing hormone (GnRH) conjugated to keyhole limpet haemocyanin and appears to be directed against amino acids 6-8 of this decapeptide. This has allowed the development of a radio-immunoassay for GnRH sensitive to 5 picograms per tube. Although it is easily measurable in hypothalamic extracts, we have failed to detect GnRH in plasma and urine from normal subjects and menopausal women.
D000818:Animals; D002369:Castration; D003429:Cross Reactions; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006065:Gonadotropins, Pituitary; D006801:Humans; D007031:Hypothalamus / Q000032:analysis; D008297:Male; D008593:Menopause; D010906:Pituitary Hormone-Releasing Hormones / Q000032:analysis* / Q000302:isolation & purification; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats
D006065:Gonadotropins, Pituitary; D010906:Pituitary Hormone-Releasing Hormones; D004958:Estradiol