title: Mouse mononuclear cell chemotaxis: description of system.
abstract: The mononuclear cell chemotaxis assay was adapted for use with normal mouse peritoneal cells. Mouse peritoneal macrophages responded well to endotoxinactivated mouse serum and to chemotactic factors produced by mouse spleen cell cultures stimulated with mitogens or specific antigen. The assay was quantitative, reproducible, and applicable to several mouse strains.
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D001500:BCG Vaccine; D002478:Cells, Cultured; D002633:Chemotaxis; D004731:Endotoxins / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice / Q000276:immunology*; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D013154:Spleen / Q000166:cytology
chemical_list: D001500:BCG Vaccine; D004731:Endotoxins; D008934:Mitogens