Lymphocyte proliferation in vitro induced by soluble protein antigens. II. Cellular requirements.
Immune guinea pig lymph node cells were fractionated on Ig anti-Ig or HSA anti-HSA affinity columns or on plastic surface in medium containing carbonyl iron. These techniques selectively removed B lymphocytes, K lymphocytes or adherent cells. The residual cells (Fc receptor-negative T lymphocytes) responded to soluble antigen in vitro in the same way or even better compared with nonfractionated cells. In addition, there was no indication that antigen-antibody complexes were superior to antigen in triggering lymph node cells or purified lymph code T lymphocytes into DNA synthesis. The results obtained suggested that memory T lymphocytes can be stimulated by antigen autonomously.
D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens; D001665:Binding Sites; D002448:Cell Adhesion; D006168:Guinea Pigs; D007141:Immunoglobulin Fc Fragments; D007156:Immunologic Memory; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000941:Antigens; D007141:Immunoglobulin Fc Fragments; D011947:Receptors, Antigen, B-Cell