Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases.
Yeast ribosomal DNA (rDNA) was digested with the restriction enzymes Hind III, Hind II and a mixture of Hind II and Hind III. The cleavage products were analyzed by electrophoresis on 1.5% agarose gels. Several distinct bands could be observed, which are derived from the redundant ribosomal transcription units. They are superimposed on a rather broad smear of background DNA, representing the heterogenous 'spacer' sequences. From the restriction maps, together with data obtained by partial digestion, a physical map for the ribosomal transcription unit in yeast could be constructed.
D004247:DNA / Q000096:biosynthesis; D004262:DNA Restriction Enzymes; D006193:Haemophilus influenzae / Q000201:enzymology; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D012270:Ribosomes / Q000032:analysis*; D012440:Saccharomyces / Q000032:analysis* / Q000378:metabolism; D014158:Transcription, Genetic
D004247:DNA; D004262:DNA Restriction Enzymes