Regulation of branched-chain aminoacyl-transfer ribonucleic acid synthetases in an ilvDAC deletion strain of Escherichia coli K-12.
Valyl-, isoleucyl-, and leucyl-transfer ribonucleic acid synthetase formation was compared in isogenic strains of Escherichia coli K-12 that differed only in that one strain carried a deletion of three genes of the ilv gene cluster, ilvD, -A, and -C. It was found that: (i) the activities of these synthetases in the deletion strain were less than those in the normal strain during growth in minimal medium supplemented with excess isoleucine, valine, and leucine, and (ii) their stability was reduced in the deletion strain during specific branched-chain amino acid limitations. The results of density-labeling experiments suggest that the in vivo stability of valyl-, isoleucyl-, and leucyl-transfer ribonucleic acid synthetases requires some product missing in the ilvDAC deletion strain.
D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis* / Q000378:metabolism; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005796:Genes; D007532:Isoleucine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D009154:Mutation; D014633:Valine / Q000378:metabolism
D002250:Carbon Radioisotopes; D007532:Isoleucine; D000604:Amino Acyl-tRNA Synthetases; D007930:Leucine; D014633:Valine