[Analysis of a small quatity of polypeptides by two-dimensional electrophoresis: application to characterization of proteins].
A new two-dimensional electrophoresis is described. The first electrophoresis is performed in a 10 p. cent gel (1 X 110 mm) in the presence of 7 M urea; the migration in the second dimension proceeds on a 20 p. cent polyacrylamide sheet (160 X 110 X 1 mm) in the presence of sodium dodecyl sulphate. This system resolves most of the polypeptides obtained by treating a very small amount (about 100 mug) of proteins with cyanogene bromide. Macromolecules can thus be quickly and easily characterized.
D000469:Alkaline Phosphatase / Q000032:analysis; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology; D008832:Microchemistry; D009113:Muramidase / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D011117:Polyribonucleotide Nucleotidyltransferase / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D014508:Urea
D010446:Peptide Fragments; D010455:Peptides; D012967:Sodium Dodecyl Sulfate; D014508:Urea; D011117:Polyribonucleotide Nucleotidyltransferase; D000469:Alkaline Phosphatase; D009113:Muramidase; D003488:Cyanogen Bromide
Analyse d'une faible quantité de polypeptides par électrophorèse à deux dimensions : application à la caractérisation des protéines