Relationship between biological activities of polymers. I. Immunogenicity, C3 activation, mitogenicity for B cells and adjuvant properties.
The effect of thirteen compounds on C3 activation, mitogenicity for B lymphocytes and on the immune response was tested. Only two of the compounds tested, polyinosinic acid and a high molecular weight polyethylenesulphate induced C3 conversion, while eleven out of the thirteen compounds (negative charged immunogenic or non-immunogenic polymers including the three C3 active compounds) stimulated thymidine incorporation in B lymphocytes. The non-charged (T-independent) immunogens dextran and polyvinylpyrrolidone neither activated C3 nor BP lymphocytes. With the exception of single-stranded polyribonucleic acids, the compounds stimulating DNA synthetis in B cells enhanced the response of the mice to a suboptimal dose of sheep red blood cells. In conclusion C3 activation, intrinsic (T-independent) immunogenicity and B-cell stimulation are not correlated properties. However, B-cell mitogenicity and adjuvanticity seem to be related.
D000276:Adjuvants, Immunologic; D000818:Animals; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003176:Complement C3; D003165:Complement System Proteins; D004247:DNA / Q000096:biosynthesis; D003911:Dextrans / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens; D011108:Polymers / Q000276:immunology*; D011119:Polynucleotides / Q000276:immunology*; D011205:Povidone / Q000276:immunology; D012756:Sheep / Q000276:immunology; D014373:Tuberculin
D000276:Adjuvants, Immunologic; D000941:Antigens; D003176:Complement C3; D003911:Dextrans; D008934:Mitogens; D011108:Polymers; D011119:Polynucleotides; D014373:Tuberculin; D003165:Complement System Proteins; D004247:DNA; D011205:Povidone