title: Replication of colicin E1 plasmid DNA added to cell extracts.
abstract: Closed-circular DNA of colicin E1 plasmid can undergo a round of semiconservative replication when added to an extract of Escherichia coli. Extracts of cells that do not carry the plasmid are able to perform complete replication of the plasmid. Replication requires de novo RNA synthesis but not protein synthesis.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002474:Cell-Free System; D003087:Colicins / Q000096:biosynthesis*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004270:DNA, Circular / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism*; D005111:Extrachromosomal Inheritance; D007700:Kinetics; D009711:Nucleotides / Q000378:metabolism; D012329:RNA, Bacterial / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D003087:Colicins; D004269:DNA, Bacterial; D004270:DNA, Circular; D009711:Nucleotides; D012329:RNA, Bacterial