title: Mating type and sporulation in yeast. I. Mutations which alter mating-type control over sporulation.
abstract: In Saccharomyces cerevisiae, meiosis and spore formation as well as mating are controlled by mating-type genes. Diploids heterozygous for mating type (aalpha) can sporulate but cannot mate; homozygous aa and alpha-alpha diploids can mate, but cannot sporulate. From an alpha-alpha diploid parental strain, we have isolated mutants which have gained the ability to sporulate. Those mutants which continue to mate as alpha-alpha cells have been designated CSP (control of sporulation). Upon sporulation, CSP mutants yield asci containing 4alpha spores. The mutant gene which allows alpha-alpha cells to sporulate is unlinked to the mating-type locus and also acts to permit sporulation in aa diploid cells. Segregation data from crosses between mutant alpha-alpha and wild-type aa diploids and vice versa indicate (for all but one mutant) that the mutation which allows constitutive sporulation (CSP) is dominant over the wild-type allele. Some of the CSP mutants are temperature-sensitive, sporulating at 32 degrees, but not at 23 degrees. In addition to CSP mutants, our mutagenesis and screening procedure led to the isolation of mutants which sporulate by virtue of a change in the mating-type locus itself, resulting in loss of ability to mate.
mesh_terms: D002467:Cell Nucleus; D004247:DNA / Q000096:biosynthesis; D004171:Diploidy; D005796:Genes; D006579:Heterozygote; D006720:Homozygote; D008540:Meiosis; D009154:Mutation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000502:physiology*; D013172:Spores, Fungal
chemical_list: D004247:DNA