Antimutator activity during mitosis by a meiotic mutant of yeast.
Diploids homozygous for the mutation spo7-1 do not exhibit net premeiotic DNA synthesis at 34 degrees C and are defective in commitment to recombination following exposure to sporulation medium. The spo7-1 mutation confers antimutator activity during mitosis at 34 degrees C, indicating that the SPO7 gene product is involved in both mitotic and premeiotic DNA metabolism. Strains bearing spo7-1 are slightly more sensitive to killing by ultraviolet light than the wild type but are proficient in UV induced mutation and mitotic intragenic recombination. The mitotic antimutator activity of spo7-1 is directed against a class of forward mutations known to occur more frequently during mitosis than meiosis.
D002250:Carbon Radioisotopes; D004247:DNA / Q000096:biosynthesis; D004171:Diploidy; D005827:Genetics, Microbial; D008540:Meiosis; D008938:Mitosis; D009154:Mutation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae; D013696:Temperature; D014316:Tritium; D014466:Ultraviolet Rays; D014498:Uracil
D002250:Carbon Radioisotopes; D014316:Tritium; D014498:Uracil; D004247:DNA