title: Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase.
abstract: Genetic studies show that Escherichia coli has three enzymes capable of phosphorylating glucose: soluble adenosine 5'-triphosphate-dependent glucokinase, which plays only a minor role in glucose metabolism; an enzyme II, called glucosephosphotransferase, with high specificity for the D-glucose configuration; and another enzyme II, called mannosephosphotransferase, with broader specificity. The former enzyme II is active on glucose and methyl-alpha-glucopyranoside, whereas the latter is active on D-glucose, D-mannose, 2-deoxy-D-glucose, D-glucosamine, and D-mannosamine. Mutations leading to loss of glucosephosphotransferase activity and designated by the symbol gpt are between the purB and pyrC markers in a locus previously called cat. The locus of mutations to loss of mannosephosphotransferase, mpt, is between the eda and fadD genes. Mutations to loss of glucokinase, glk, are between the ptsI and dsd genes.
mesh_terms: D002874:Chromosome Mapping; D003847:Deoxyglucose / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D005941:Glucokinase / Q000096:biosynthesis*; D005944:Glucosamine / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D009154:Mutation; D010770:Phosphotransferases / Q000096:biosynthesis*; D011995:Recombination, Genetic; D014161:Transduction, Genetic
chemical_list: D005632:Fructose; D008353:Mannitol; D003847:Deoxyglucose; D010770:Phosphotransferases; D005941:Glucokinase; D005947:Glucose; D007785:Lactose; D005944:Glucosamine; D005990:Glycerol; D008358:Mannose