Different surface glycoprotein patterns on human T-, B- and leukemic-lymphocytes.
We have labelled the exposed surface glycoproteins of human blood T- and B-lymphocytes and cells from patients with chronic lymphocytic leukemia by the galactose oxidase-tritiated sodium borohydride method. The labelled glycoproteins were separated by polyacrylamide slab gel electrophoresis and visualized by autoradiography. The T- and B-lymphocytes show different and characteristic surface glycoprotein patterns. The surface glycoprotein patterns of the leukemic cells differ from those of normal, non-malignant lymphocytes. A relationship between the altered surface glycoprotein pattern of leukemic cells and the expression of leukemia-associated antigens is discussed.
D000368:Aged; D001402:B-Lymphocytes / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D005692:Galactose Oxidase / Q000494:pharmacology; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009439:Neuraminidase / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism*
D006023:Glycoproteins; D005692:Galactose Oxidase; D009439:Neuraminidase