[Effect of embichin (HN2) and its monofunctional analog (HN1) on chromatin and DNA matrix activity in an in vitro RNA-polymerase system].
Embichin inhibited the matrix activity of chromatin and DNA in the RNA-polymerase system in vitro much more than its monofunctional analogue. Chromatin possessed a greater sensitivity to the action of embichin in comparison with the deproteinised DNA. However,  with the action of a monofunctional embichin analogue there was a greater reduction of the matrix activity of DNA in comparison with chromatin. The depression mechanism of the matrix activity of chromatin with the action of embichin was apparently associated with the capacity of the latter to form the DNA-protein bonds in the chromatin composition.
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002843:Chromatin / Q000187:drug effects*; D004247:DNA / Q000502:physiology*; D012321:DNA-Directed RNA Polymerases / Q000502:physiology*; D004926:Escherichia coli / Q000201:enzymology; D066298:In Vitro Techniques; D008466:Mechlorethamine / Q000031:analogs & derivatives / Q000494:pharmacology*; D010597:Pharmacogenetics; D011485:Protein Binding / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D013950:Thymus Gland; D013997:Time Factors
D002843:Chromatin; D008466:Mechlorethamine; D004247:DNA; D012321:DNA-Directed RNA Polymerases
Vliianie émbikhina (HN2) i ego monofunktsional'nogo analoga (HN1) na matrichnuiu aktivnost' khromatina i DNK  v RNK-polimeraznoĭ sisteme in vitro