Purification and some properties of malarial pigment.
Malarial pigment from erythrocytes infected with Plasmodium berghei was purified by treatment with sodium dodecyl sulphate solution, followed by incubation with Pancreatin. The purified pigment retained the apparently crystalline form of pigment within the parasite, rotated polarised light and had the same solubility characterisation as crude malarial pigment. It contained about 1% iron, all of which could be accounted for in terms of haemin. The iron of the pigment molecule is oxidised by the parasite to the ferric state.
D000818:Animals; D004912:Erythrocytes / Q000469:parasitology; D006427:Hemin / Q000032:analysis; D007501:Iron / Q000032:analysis; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D010084:Oxidation-Reduction; D010860:Pigments, Biological / Q000302:isolation & purification*; D010962:Plasmodium berghei / Q000032:analysis* / Q000648:ultrastructure; D013053:Spectrophotometry
D010860:Pigments, Biological; D006427:Hemin; D007501:Iron