Electrophoretic fractionation of guniea pig lymphocytes: evidence for different subsets of T and B cells in spleen and lymph node.
Guinea pig lymph node and blood lymphocytes have been physically fractionated in preparative cell electrophoresis into two functionally viable populations, the high mobility cell population (HMC) and the low mobility cell population (LMC). By using cell surface markers and functional tests known to be specific for T and B lymphocytes, respectively, it is shown that the T lymphocytes localize in the HMC population and the B lymphocytes in the LMC population. The spleen lymphocytes do not separate into the two populations. They move into one single broad peak containing both T and B cells. This finding indicates the presence of electrokinetically different subsets of T and B lymphocytes in the spleen on one hand and in the lymph node and blood on the other hand.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001773:Blood Cells; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000494:pharmacology; D004586:Electrophoresis; D004926:Escherichia coli / Q000276:immunology; D006168:Guinea Pigs; D007104:Immune Adherence Reaction; D037102:Lectins; D008070:Lipopolysaccharides; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D008214:Lymphocytes / Q000276:immunology; D011135:Polysaccharides, Bacterial; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology
D037102:Lectins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D003520:Cyclophosphamide