[Immunofluorescence technique in diagnosis of trichinellosis in swine. II. Comparative investigations with the tube test and the cryostate method (author's transl)].
A series of comparative IF tests have been made on serum samples from 9 SPF pigs experimentally infected with Trichinella spiralis (50-500 larvae per animal). The techniques applied included a tube test (antigen: whole Trichinella larvae), and a cryostat test (antigen: sections of Trichinella larvae). With the cryostat test a positive reaction was recorded in 2 animals already on the 6th day and in 5 animals on the 13th day p.i., while with the tube test such reaction was not demonstrated until the 27th day p.i. (2 animals). A potential risk of cross-reaction of unknown origin seems, however, to limit the application of the cryostat test to SPF- pigs.
D000818:Animals; D005455:Fluorescent Antibody Technique; D008722:Methods; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology; D014235:Trichinellosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
Immunofluorescensteknik ved påvisning af trichinellose hos svin. II. Sammenlignende undersøgelser mellem reagensglasmetoden og cryostatmetoden