Separation of rosette-forming cells with the aid of an immunosorbent based on sephadex G-25 and G-75.
A method of separating rosette-forming cells on columns with an immunosorbent based on sephadex G-75 is described and has been tested. The protein antigen (bovine serum albumin-BSA) is attatched by covalent bonds to the surface of the Sephadex granules oxidized by sodium periodate. Cells with receptors on their surface were tested by the rosette method. Up to 88% of the rosette-forming cells were retained on a column packed with BSA-Sephadex granules. Nonspecific retention of the cells was relatively small, about 4%.
D000327:Adsorption; D000818:Animals; D000917:Antibody Formation; D001666:Binding Sites, Antibody; D002469:Cell Separation / Q000379:methods*; D006462:Hemolytic Plaque Technique; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012685:Sepharose; D012710:Serum Albumin, Bovine; D013154:Spleen / Q000166:cytology*
D012710:Serum Albumin, Bovine; D012685:Sepharose