[Phytomitogen-induced lymphocyte transformation - standardization and diagnostic value (author's transl)].
Lymphocytes can transform into blasts. This basic biological property is the prerequisite for many reactions of the cellular immune system. Therefore, lymphocyte transformation tests appear to be a valuable diagnostic approach to define primary and secondary immundeficiency associated with different diseases. The validity of the test system is based on a standardization wich can be achieved sufficiently with regard to culture conditions and measurement of DNA-synthesis. But for phytomitogen-induced lymphocyte transformation a cooperative system of lymphocytes and macrophages is necessary. The results are influenced to a high degree by different cell separation procedures. Therefore, the interpretation of abnormal lymphocyte transformation data, induced unspecifically mitogens, has to take into account alteration of lymphocyte function, different T-/B-cell ratios and changing counts and helper effects of macrophages in order to define a defective lymphocyte transformation more precisely.
D000888:Antibodies, Anti-Idiotypic; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D002448:Cell Adhesion; D002478:Cells, Cultured; D003208:Concanavalin A; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D037102:Lectins; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D037102:Lectins; D008070:Lipopolysaccharides; D003208:Concanavalin A; D004247:DNA
Zur Standardisierung und diagnostischen Bedeutung der phytomitogen-induzierten lymphozytentransformation