Syngeneic adoptive immunotherapy and chemoimmunotherapy of a Friend leukemia: requirement for T cells.
The aim of the study was to determine which cell mediates adoptive immunotherapy and chemoimmunotherapy of a syngeneic transplantable Friend virus-induced leukemia (FBL-3). An adoptive immunotherapy model was developed in which adult C57BL/6 mice given a lethal dose (10(4)) of FBL-3 on day 0 were saved by treatment on day 1 with C57BL/6 spleen cells or peritoneal exudate cells (PEC) immune to FBL-3. Cells passed through a nylon wool column to remove B cells and macrophages or treated with carbonyl iron to remove phagocytic cells remained effective, whereas cells treated with anti-theta serum and complement were far less effective. For adoptive chemoimmunotherapy, mice inoculated with 10(7) FBL-3 were treated 5 days later with cyclophosphamide (CY) plus immune spleen cells. CY, with or without non-immune cells, prolonged survival but all mice died with leukemia, whereas mice given CY plus immune cells survived tumor-free. As an adjunct to CY, immune cells passed through nylon wool or treated with carbonyl iron remained quite effective whereas cells treated with anti-theta serum and complement were far less effective. Thus, immune thymus-derived lymphocytes were required for the adoptive immunotherapy of an early leukemia or chemoimmunotherapy of a disseminated leukemia.
D000818:Animals; D000961:Antilymphocyte Serum / Q000032:analysis; D002845:Chromatography; D003520:Cyclophosphamide / Q000627:therapeutic use; D003601:Cytotoxicity Tests, Immunologic; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004358:Drug Therapy / Q000379:methods*; D005622:Friend murine leukemia virus / Q000276:immunology*; D007111:Immunity, Cellular; D007115:Immunization Schedule; D007167:Immunotherapy / Q000379:methods*; D007502:Iron Chelating Agents / Q000276:immunology; D007942:Leukemia, Experimental / Q000628:therapy*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009757:Nylons; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000378:metabolism*; D014184:Transplantation, Homologous; D014935:Wool
D000961:Antilymphocyte Serum; D007502:Iron Chelating Agents; D009757:Nylons; D003520:Cyclophosphamide