The relationship between tumorigenicity, growth in agar and fibrinolytic activity in a line of human osteosarcoma cells.
The TE-85 osteosarcoma cell line has several of the in vitro properties of malignant cells, including colony formation in agar, but has low extracellular fibrinolytic activity and no capacity to form tumors in ATS-treated hamsters. Some TE-85 cells clones (clones 2, 4 and 6) have increased fibrinolytic activity but do not form tumors in hamsters. TE-85 cells infected with mammalian transformation-defective viruses have low (FeLV) or increased (RD-114 virus) levels of fibrinolytic activity and do not form tumors in hamsters. TE-85 cell either nonproductively infected with Ki-MSV or productively infected with M-MSV (RD-114), have fibrinolytic activity and can form tumors in hamsters. The MSV gene(s) but not colony formation in agar or extracellular fibrinolytic activity appears to be capable of rendering TE-85 cells tumorigenic in ATS-treated hamsters.
D000362:Agar; D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D006224:Cricetinae; D003470:Culture Media; D005342:Fibrinolysis; D006801:Humans; D007165:Immunosuppression Therapy; D066298:In Vitro Techniques; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D009858:Oncogenic Viruses; D012516:Osteosarcoma / Q000097:blood / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology; D014183:Transplantation, Heterologous
D000961:Antilymphocyte Serum; D003470:Culture Media; D000362:Agar