The need for transcription and translation for differentiation of bone marrow cells by thymic factors in man.
1. Two small populations of human marrow cells are differentiated to cells bearing T-cell markers by Thymosin F3 or F5. 2. Analysis with metabolic inhibitors indicates that transcription and translation of RNA are needed for induction of T cell markers (layer III). 3. a) At 4 hours incubation RNA synthesis is enhanced in layers III, IV and V and inhibited in layers I + II. b) At 20 hours incubation RNA synthesis is markedly enhanced in all cell layers. 4. a) At 5 hours incubation protein synthesis is suppressed in layers I + II and III, but not in IV and V. b) At 20 hours incubation protein synthesis is markedly increased only in layer III.
D000546:Amanitins / Q000494:pharmacology; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology; D006801:Humans; D007104:Immune Adherence Reaction; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000502:physiology*; D014158:Transcription, Genetic
D000546:Amanitins; D003609:Dactinomycin; D011691:Puromycin; D012313:RNA