[Immunological activities of rat lymphocytes. III. --Isolation of differing subpopulations of lymphocytes by two techniques of T cell separation].
In previous studies we have isolated by density gradient separation a population of relatively dense T lymphocytes capable of inhibiting the mitogenic response to concanavalin A (ConA) and phytohaemagglutinin. In this study we compared two current methods of cell separation for their relative yield in T lymphocytes able or not to respond to Con A. The present results show that cells passed through nylon wool columns--in contrast to those obtained by removal of lymphocytes which bind erythrocyte-antibody-complement complex-partially lose their ability to respond to ConA. The response lost by passage through nylon wool columns can be restored by centrifugation of the T cell suspension on a gradient of "Ficoll-Hypaque". This suggests that methods of T cells purification using density gradient separation may remove the more dense suppressor cells.
D000818:Animals; D000937:Antigen-Antibody Reactions; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003165:Complement System Proteins / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051381:Rats / Q000276:immunology; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology*
D003208:Concanavalin A; D003165:Complement System Proteins
Activités immunologiques des lymphocytes de rat. III.--Obtention de sous-populations de cellules différentes par deux techniques de purification des lymphocytes T