title: Detection of T-precursor cells in human bone marrow and foetal liver.
abstract: Human lymphoid cells that have been incubated with conditioned medium from confluent monolayers of human thymic epithelium (HTCM) show an increase in cells forming rosettes with sheep erythrocytes at 4 degrees C (E-rosettes). Those cells demonstrating this in vitro conversion have been interpreted to be T-cell precursors. Separation of human bone marrow cells on discontinuous bovine serum albumin (BSA) gradients, and on a single-step 23% BSA gradient showed enrichment of these T-precursor cells, not only in bone marrow, but also in human foetal liver cells. Bone marrow precursor cells from a patient with Wiskott-Aldrich syndrome (T-cell deficiency) showed a normal in vitro response to HTCM, but no response was seen in cells from a patient with severe combined immunodeficiency disease.
mesh_terms: D000361:Agammaglobulinemia / Q000473:pathology; D001854:Bone Marrow Cells; D002648:Child; D046508:Culture Techniques; D006801:Humans; D007104:Immune Adherence Reaction; D007153:Immunologic Deficiency Syndromes / Q000473:pathology; D008099:Liver / Q000166:cytology / Q000196:embryology*; D008231:Lymphopenia / Q000473:pathology; D013601:T-Lymphocytes / Q000502:physiology*