title: Detection of fungi in clinical specimens by phase-contrast microscopy.
abstract: During 1973 and 1974, the following fungi were detected in clinical specimens by using phase-contrast microscopy: Blastomyces dermatitidis, 5; Coccidioides immitis, 3; Cryptococcus neoformans, 11; other yeasts 918; dermatophytes, 863; Mucor species, 1; and Aspergillus fumigatus, 16. This technique allows rapid detection and, in many instances, immediate identification of fungi in clinical specimens.
mesh_terms: D003883:Arthrodermataceae / Q000302:isolation & purification; D001230:Aspergillus / Q000302:isolation & purification; D001758:Blastomyces / Q000302:isolation & purification; D001826:Body Fluids / Q000382:microbiology; D003045:Coccidioides / Q000302:isolation & purification; D003455:Cryptococcus neoformans / Q000302:isolation & purification; D003937:Diagnosis, Differential; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D008858:Microscopy, Phase-Contrast; D009089:Mucor / Q000302:isolation & purification; D009181:Mycoses / Q000175:diagnosis*; D012867:Skin / Q000382:microbiology; D013183:Sputum / Q000382:microbiology; D013492:Suppuration / Q000382:microbiology; D014556:Urine / Q000382:microbiology; D015003:Yeasts / Q000302:isolation & purification