title: Mapping of colicin E2 and colicin E3 plasmid deoxyribonucleic acid EcoR-1-sensitive sites.
abstract: Colicin plasmids E2 and E3 (Col E2 and Col E3) deoxyribonucleic acid (DNA) has been shown to contain, respectively, two and three EcoR1 restriction endonuclease-sensitive sites. This was determined by measuring the DNA fragments generated after EcoR1 endonuclease treatment by agarose gel electrophoresis and electron microscopy. The structure of heteroduplex Col E2-col E3 DNA molecules formed from EcoR1-generated fragments permitted a localization of the EcoR1-sensitive sites on the plasmid chromosomes.
mesh_terms: D001665:Binding Sites; D003087:Colicins / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000378:metabolism*; D004270:DNA, Circular / Q000378:metabolism*; D004586:Electrophoresis; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005111:Extrachromosomal Inheritance; D005559:Formamides; D008854:Microscopy, Electron; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation
chemical_list: D003087:Colicins; D004269:DNA, Bacterial; D004270:DNA, Circular; D005559:Formamides; D004720:Endonucleases