[The morphological and functional integrity of cryo-preserved leukocytes].
Human peripheral blood lymphocytes were frozen by a simplified method which is described. Light and electron microscopic investigations of the frozen-thawed cells showed no significant morphological changes compared to the fresh cells; comparison of E-rosette formation and nonspecific acid esterase activity before and after cryoconservation afford evidence of a possible slight change in the percent relationship of T- and B-cells, whereas thymidine uptake values within normal range after stimulation with PHA and homologous cells demonstrate the functional integrity of the lymphocytes.
D001402:B-Lymphocytes / Q000276:immunology; D001793:Blood Preservation / Q000592:standards*; D005615:Freezing / Q000379:methods*; D006801:Humans; D007962:Leukocytes; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism / Q000648:ultrastructure; D013601:T-Lymphocytes / Q000276:immunology
Untersuchungen zur morphologischen und funktionellen Integrität kryokonservierter Leukozyten