[Purification of an anionic trypsin from the gastric juice of the crayfish Cambarus affinis say by means of affinity chromatography].
Beside other digestion enzymes the crayfish Cambarus affinis SAY possess an anionic trypsin. This enzyme could be separated from all the other proteins of the crude gastric juice by a single affinity chromatographic step on immobilized natural inhibitor. The dissociation of the thus produced enzyme-inhibitor complex was achieved by specific elution with ben-amidine. The soluble benzamidine-trypsin-complex was dialyzed and separated by gelfiltration on Sephadex G-50. Of the ligands used Trasylol proved to be the most effective one. Trypsin isolated by this ligand was in a discelectrophoretic homogeneous state. On the other hand, trypsin purified by Soybean trypsin inhibitor and Contrykal contains impurities.
D000818:Animals; D007611:Aprotinin; D003400:Astacoidea; D001550:Benzamidines; D001560:Benzidines; D002846:Chromatography, Affinity; D003488:Cyanogen Bromide; D005750:Gastric Juice / Q000201:enzymology; D005898:Glass; D008722:Methods; D012685:Sepharose; D014357:Trypsin / Q000302:isolation & purification*
D001550:Benzamidines; D001560:Benzidines; D012685:Sepharose; D007611:Aprotinin; D014357:Trypsin; D003488:Cyanogen Bromide
Reinigung eines anionischen Trypsins aus dem Magensaft des Flusskrebses Cambarus affinis SAY durch Affinitätschromatographie