title: Effect of microbial substances of different origins on the growth of Mycobacterium leprae.
abstract: Mycobacterium leprae multiplied in media enriched with substances originating from other mycobacteria, from non-acid-fast Actinomycetales or from gram-positive or gram-negative Eubacteriales. Most of the M. leprae strains did not grow on a synthetic medium containing the amino acids present in M. smegmatis, but the growth-promoting effect of sonic extracts of this organism indicated that substances of bacterial origin, other than amino acids, do act as growth factors. The identification of the growing, acid-fast microorganisms with M. leprae was verified by their ability to oxidize D-3,4-dihydroxyphenylalanine (D-dopa test). M. leprae did not multiply on Nakamura medium unless at least 40% of medium NM7 enriched by bacterial substances was present. Adequate aeration was essential for multiplication of M. leprae in enriched NM7 and No. 3 media.
mesh_terms: D001407:Bacillus; D001419:Bacteria; D003470:Culture Media; D004926:Escherichia coli; D006133:Growth Substances; D007711:Klebsiella pneumoniae; D009161:Mycobacterium; D009166:Mycobacterium leprae / Q000254:growth & development*; D013210:Staphylococcus; D013302:Streptomyces
chemical_list: D003470:Culture Media; D006133:Growth Substances