Qualitative and quantitative study of the antigens and antibodies of the HL-A system-automatic fluorochrome method.
It was possible to obtain a successful evaluation of the quantity of HL-A antigens on lymphocytes using a fluorochromatic test with double marking by fluorescine diacetate and ethidium bromide. It was also possible to detect anti-HL-A antibodies, to study their cross-reactions and to perform tissue typing using this automatic, sensitive and repeatable method.
D000042:Absorption; D001331:Automation; D001965:Bromides; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D004996:Ethidium; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique / Q000379:methods; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology; D008856:Microscopy, Fluorescence
D001965:Bromides; D005452:Fluoresceins; D006649:Histocompatibility Antigens; D007518:Isoantibodies; D004996:Ethidium