Relation of beta-lactamase activity to antimicrobial susceptibility in Serratia marcescens.
One-hundred clinical isolates of Serratia marcescens were tested for susceptibility to cephalothin, carbenicillin, ticarcillin, ampicillin, and cefoxitin. The majority of the 100 isolates (>/=70%) were susceptible to carbenicillin, ticarcillin, and cefoxitin; less than one-half were susceptible to ampicillin; none were susceptible to cephalothin. Ten isolates from the 100 organisms tested were selectively assayed for their beta-lactamase activity. Enzyme activity was measured using either iodometric or spectrophotometric methods, and the microbiological assay technique. It was concluded that beta-lactamase production was not the sole determinant in beta-lactam antibiotic resistance. Resistance without demonstrable beta-lactamase was evident in strains for cephalothin, ampicillin, and cefoxitin. In addition, one strain which was susceptible to all antibiotics except cephalothin, elaborated considerable beta-lactamase activity.
D000581:Amidohydrolases / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002510:Cephalosporinase / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D010858:Pigmentation; D012706:Serratia marcescens / Q000187:drug effects* / Q000201:enzymology
D000900:Anti-Bacterial Agents; D000581:Amidohydrolases; D002510:Cephalosporinase