[B-lymphocytes subpopulation. Rapid identification with a histoimmunological method (author's transl)].
After extraction of tissues (spleen, thymus, blood) B cell bearing surface immunoglobulins were specifically labelled with Fab-peroxidase conjugate in light and electron microscopy. Positive and negative labelled lymphocyte were counted. This procedure allowed us to quantitate B and T cell in different diseases in peripheral blood and within lesions. It may represent a new immunopathological approach.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007158:Immunologic Techniques; D051379:Mice; D008854:Microscopy, Electron; D010544:Peroxidases; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology*
D007140:Immunoglobulin Fab Fragments; D011947:Receptors, Antigen, B-Cell; D010544:Peroxidases
Technique immunohistochimique rapide d'identification des cellules lymphoïdes de la lignée B