title: Detergent solubilisation of baboon histocompatibility antigens and their use in prolonging liver allograft survival.
abstract: Histocompatibility antigens have been solubilised from baboon spleen cell membranes by brief exposure to sodium deoxycholate (DOC).  Preparations contained a high molecular weight species (DOCH-Ag) excluded by Sephadex G-200, and a low molecular weight species (DOCL-Ag) which eluted in the included fraction (molecular weight 46,000).  The low molecular weight antigen was stable and was not sedimented by high speed centrifugation even after dialysis against a low molar salt solution.  It elicited a delayed-type hypersensitivity response in sensitised baboons but not in normal animals.  DOCL-Ag cultured in vitro with sensitised allogeneic lymphocytes produced a 1.3- to 24.9-fold stimulation, whereas when cultured with normal lymphocytes the maximum stimulation index was 2.  Intravenous administration of donor DOCL-Ag (2 mg/kg) at the time of transplantation and without additional immunosuppression significantly prolonged the mean survival time of baboon liver allografts.  The same treatment with donor DOCH-Ag did not prolong graft survival.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004247:DNA / Q000032:analysis; D003902:Detergents / Q000494:pharmacology*; D005374:Filtration; D006649:Histocompatibility Antigens; D006968:Hypersensitivity, Delayed / Q000276:immunology; D016031:Liver Transplantation; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D010215:Papio; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D013154:Spleen / Q000166:cytology; D014181:Transplantation Immunology; D014184:Transplantation, Homologous; D014461:Ultracentrifugation
chemical_list: D003902:Detergents; D006649:Histocompatibility Antigens; D011506:Proteins; D012313:RNA; D004247:DNA