Role of lysosomal constituents of neutrophils in corneal graft reaction.
Band 2 protein exists in a firmly bound state in lysosomes, and is released when the cells concerned are specifically stimulated. The necessary stimulus can originate from different kinds of immunogenic interactions. The objective of this study was to determine whether during corneal graft reaction Band 2 protein could be detected in the host's cornea and aqueous humour. Band 2 protein was detected in the reacting host corneas (8 out of 23 eyes) and the aqueous humours (2 out of 23 eyes), mainly during the second and third postoperative weeks when parallel histological studies showed an accumulation of neutrophils in the graft bed. The severity of graft reaction appeared to run parallel to the amount of free Band 2 protein in the host cornea. After this period, in spite of the presence of a large number of neutrophils in the reacting host cornea, Band 2 protein was hardly detectable. This study indicates that the necessary stimulus for the secretion of Band 2 protein from rabbit neutrophils is present in the early stages of corneal xenograft reaction. The presence of the protein in the host cornea may be a factor in the development of the vascular response associated with the corneal graft reaction.
D000818:Animals; D001082:Aqueous Humor / Q000032:analysis; D002417:Cattle / Q000276:immunology; D003315:Cornea / Q000032:analysis; D016039:Corneal Transplantation; D004591:Electrophoresis, Polyacrylamide Gel; D005966:Glucuronidase / Q000032:analysis; D006084:Graft Rejection; D008247:Lysosomes / Q000032:analysis / Q000378:metabolism*; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D011817:Rabbits; D014183:Transplantation, Heterologous
D011506:Proteins; D005966:Glucuronidase