title: In vitro lymphocyte transformation in the development of experimental allergic thyroiditis.
abstract: In vitro lymphocyte transformation to homologous thyroid antigen has been studied in cultures of lymph node cells obtained from 97 guinea pigs at weekly intervals after a single injection of homologous thyroid extract in complete Freund's adjuvant, using a method for measurement of 3-H-thymidine incorporation. Lymph node cell cultures from animals 1 week after sensitization showed a significant increase in thymidine incorporation after the addition of antigen. Two weeks after sensitization the stimulation was maximal, and then a lower but significant response continued during the next 5 weeks. There was no correlation between lymphocyte transformation and the level of circulating antibodies throughout the post-sensitization period examined. The early lymphocyte transformation contrasted with the later development of macrophage migration inhibitory factor activity.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D005260:Female; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis; D008264:Macrophages / Q000276:immunology; D013634:Tannins; D013936:Thymidine / Q000378:metabolism; D013966:Thyroiditis / Q000276:immunology*; D014020:Tissue Extracts; D014316:Tritium
chemical_list: D000906:Antibodies; D008263:Macrophage Migration-Inhibitory Factors; D013634:Tannins; D014020:Tissue Extracts; D014316:Tritium; D013936:Thymidine