Rifampin inhibition of bacteriophage phiX174 parental replicative-form DNA synthesis in an Escherichia coli dnaC mutant.
The Escherichia coli dnaC protein is not absolutely required in vivo for bacteriophage phiX174 parental replicative-form synthesis (Kranias and Dumas, 1974). However, when rifampin is present at a concentration that inhibits DNA-dependent RNA polymerase, phiX174 parental replicative-form synthesis is dependent on the dnaC protein activity. We conclude that E. coli DNA-dependent RNA polymerase can substitute for the dnaC protein in phiX174 parental replicative-form DNA synthesis, presumably in its initiation. The implications of this result with respect to the in vitro synthesis of the complementary strand of phiX174 DNA are discussed.
D001426:Bacterial Proteins / Q000378:metabolism; D003090:Coliphages / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005796:Genes; D009154:Mutation; D012293:Rifampin / Q000494:pharmacology*; D013696:Temperature
D001426:Bacterial Proteins; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin