[Method of isolating pure cultures of mesophilic, thermotolerant and thermophilic methane-utilizing bacteria].
The paper describes a technique for isolation of pure cultures of various physiological groups (mesophilic, thermotolerant, and thermophilic) of methylotrophs bacteria. The technique is based on the application of solid media containing simultaneously two sources of carbon: elective for the accompanying microflora and elective for the obligate methylotrophs. If the culture to be studied is inoculated into such a medium, the following colonies will be found: (1) the initial culture; (2) pure cultures of the methylotrophs; and (3) the accompanying microflora. The paper presents a scheme for isolation of pure cultures of the methylotrophs, criteria for controlling their purity, recommendations for the composition of growth media which would not inhibit the growth of the obligate methylotrophs by organic substrates. The technique may also be used for the isolation of pure cultures of other bacteria (mycobacteria, lithotrophs, etc.) which are difficult to be separated from the accompanying microflora.
D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D003470:Culture Media; D008697:Methane / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D008697:Methane
Metod vydeleniia chistykh kul'tur mezofil'nykh, termotolerantnykh i termofil'nykh metanispol'zuiushchikh bakteriĭ