Permeability lesions in male Escherichia coli infected with bacteriophage T7.
The abortive development of bacteriophage T7 in E. coli cells carrying F factors has previously been attributed to a lack of virus-directed modification of ribosomes in such cells. We find it unnecessary to postulate such translational control to explain the failure of T7 development. Instead, there is a general cessation of macromolecular syntheses around 8 min after T7 infection of F' cells. This cessation is correlated with a sudden outflow of the entire acid-soluble pool of phosphorus-containing compounds and loss of the ability to accumulate amino acids. Manifestation of these defects requires expression of at least one T7 gene and one episomal gene.
D001345:Autoradiography; D001692:Biological Transport; D002855:Chromatography, Thin Layer; D003090:Coliphages; D004267:DNA Viruses; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D005827:Genetics, Microbial; D005973:Glutamine / Q000378:metabolism; D007700:Kinetics; D009154:Mutation; D009711:Nucleotides / Q000096:biosynthesis; D010710:Phosphates / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D013431:Sulfates / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D009711:Nucleotides; D010710:Phosphates; D013431:Sulfates; D014764:Viral Proteins; D005973:Glutamine; D012967:Sodium Dodecyl Sulfate