title: Luteinizing hormone and androgens in the bovine fetus after gonadotropin-releasing hormone.
abstract: After an intra-arterial injection of 10 mug GnRH into four bovine male fetuses, serum LH increased (P less than 0.01) 2-fold by 15 min and plateaued at approximately 11 ng/ml from 60 to 180 min. After GnRH in six female fetuses, LH increased (P less than 0.01) 7-fold by 15 min and plateaued at approximately 17 ng/ml from 60 to 180 min. Maternal serum LH was not significantly influenced (P greater than 0.05) by sex of fetus or administration of GnRH to the fetus. After GnRH in male fetuses, serum testosterone increased (P less than 0.05) and remained 2-fold greater than basal levels through 180 min. In contrast, serum testosterone from female fetuses averaged 370 +/- 60 pg/ml at injection and was unchanged after GnRH. Androgen synthesis was significantly increased during in vitro incubation of fetal testicular explants by the addition of LH. We conclude that GnRH causes release of LH from the bovine fetal pituitary as early as 120 days of age and that the testes respond with testosterone secretion after LH stimulation.
mesh_terms: D000735:Androstenedione / Q000096:biosynthesis* / Q000097:blood; D000818:Animals; D001158:Arteries; D002417:Cattle; D005260:Female; D005312:Fetal Blood; D005333:Fetus / Q000378:metabolism*; D005865:Gestational Age; D066298:In Vitro Techniques; D007269:Injections, Intra-Arterial; D007986:Luteinizing Hormone / Q000096:biosynthesis* / Q000097:blood / Q000494:pharmacology; D008297:Male; D010902:Pituitary Gland / Q000032:analysis; D010906:Pituitary Hormone-Releasing Hormones / Q000494:pharmacology*; D010920:Placenta / Q000098:blood supply; D011247:Pregnancy; D013268:Stimulation, Chemical; D013737:Testis / Q000196:embryology / Q000378:metabolism; D013739:Testosterone / Q000096:biosynthesis* / Q000097:blood; D013997:Time Factors
chemical_list: D010906:Pituitary Hormone-Releasing Hormones; D013739:Testosterone; D000735:Androstenedione; D007986:Luteinizing Hormone