The present status of prenatal detection of neural tube defects.
In experimentally induced myelocele in rats, efforts to find neural cells in amniotic fluid (AF) were unsuccessful. Creatine phosphokinase (CPK) and aldolase concentrations studied in serum of 118 and cerebrospinal fluid (CSF) in 9 patients with myelomeningocele showed serum CPK to be significantly elevated and more responsive to additional muscle injury than aldolase, but both enzymes appeared in lower concentrations in patients with myelomeningocele than those with infantile atrophy or cerebral palsy. In CSF, CPK, and aldolase concentrations averaged 4.2 I.U. and 2.7 S.L.U. per milliliter, respectively. Significant CPK elevation (p less than 0.001) was also found in AF from myeloschitic fetuses and maternal rat serum. Although these findings suggest that increased CPK concentration is an indicator of myelocele in rats, the technique is impractical for prenatal detection of human fetus occurs too late in gestation. This does not, however, preclude the value of CPK for detecting onset of paraparesis. In all myeloschitic human fetuses, the CSF communicates directly with AF for at least 3 to 4 weeks. This implies that CSF is probably the principal source of increased alpha-fetoprotein concentration encountered in AF of all pregnancies with NTD. When biological variables are recognized, it is evident that increased concentration of amniotic fluid alpha fetoprotein is a reliable indicator of fetuses with open myelocele and/or anenciphalus.
D000293:Adolescent; D000328:Adult; D000653:Amniotic Fluid / Q000032:analysis; D000818:Animals; D002547:Cerebral Palsy / Q000175:diagnosis; D002648:Child; D002675:Child, Preschool; D003402:Creatine Kinase / Q000032:analysis / Q000134:cerebrospinal fluid; D004805:Ependyma / Q000166:cytology; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis*; D005326:Fetal Proteins / Q000032:analysis; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis / Q000134:cerebrospinal fluid; D005865:Gestational Age; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008431:Maternal-Fetal Exchange; D008588:Meningocele / Q000175:diagnosis; D008591:Meningomyelocele / Q000139:chemically induced; D009132:Muscles / Q000201:enzymology; D009135:Muscular Diseases / Q000175:diagnosis; D009421:Nervous System Malformations; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011296:Prenatal Diagnosis / Q000379:methods*; D051381:Rats
D005326:Fetal Proteins; D003402:Creatine Kinase; D005634:Fructose-Bisphosphate Aldolase