Disparity in the production of lymphoblastogenesis inhibition factor by cultured human B and T lymphoid cell lines.
A comparative study of inhibitory effects of cell-free supernatants from cultured human B and T lymphoid cell lines on lymphocyte blastogenesis indicated that the inhibitory effect of supernatant from B lymphoid cells on lymphocyte blastogenesis was significantly higher than that of supernatant from T lymphoid cells or from non-lymphoid neoplastic cells. The inhibitory effect of supernatant was reversible and dose-related. The inhibitory effect gradually diminished with time when the supernatant from B lymphoid cells was added to the culture, 1-3 days after the beginning of cultures. The supernatant of human B lymphoid cells was also found to be highly active in affecting the mouse thymus cell response. The biological nature of this inhibitory factor has not been defined. Both B lymphoid cell lines used in the present study contained Epstein-Barr virus (EBV) genomes while the T-cell line and the non-lymphoid neoplastic cell lines were free of EBV genomes. Sensitivity of the supernatant of B lymphoid cells to u.v. irradiation and heat suggests the possibility that the EBV genomes released into the culture medium may be responsible for inhibition of lymphocyte blastogenesis; resistance of this supernatant to DNase suggests that the EBV genomes may be double-stranded DNA.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002474:Cell-Free System / Q000528:radiation effects; D003208:Concanavalin A / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000494:pharmacology; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology; D011402:Pronase / Q000494:pharmacology; D012260:Ribonucleases / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology
D037102:Lectins; D003208:Concanavalin A; D003851:Deoxyribonucleases; D012260:Ribonucleases; D009439:Neuraminidase; D014357:Trypsin; D011402:Pronase