Synthesis of nitrate reductase components in chlorate-resistant mutants of Escherichia coli.
Specific antibody to purified nitrate reductase from Escherichia coli was used to identify enzyme components present in mutants which lack functional nitrate reductase. chlA and B mutants contained all three subunits present in the wild-type enzyme. Different peptides with a broad range of molecular weights could be precipitated from chlCmutants, and chlE mutants contained either slightly degraded enzyme subunits or no precipitable protein. No mutants produced significant amounts of cytoplasmic enzyme. The chlA and B loci are suggested to function in the synthesis and attachment of a molybdenum-containing factor. The chlC locus is suggested to be the structural gene for nitrate reductase subunit A and chlE is suggested to be involved in the synthesis of the cytochrome b1 apoprotein.
D000693:Anaerobiosis; D000937:Antigen-Antibody Reactions; D001059:Apoproteins / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology; D011232:Chemical Precipitation; D002704:Chlorates / Q000494:pharmacology*; D003580:Cytochromes / Q000096:biosynthesis; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D006358:Hot Temperature; D008970:Molecular Weight; D009154:Mutation; D009565:Nitrate Reductases / Q000096:biosynthesis* / Q000302:isolation & purification; D010452:Peptide Biosynthesis; D011092:Polyethylene Glycols; D012995:Solubility
D001059:Apoproteins; D001426:Bacterial Proteins; D002704:Chlorates; D003580:Cytochromes; D011092:Polyethylene Glycols; D009565:Nitrate Reductases