Temporal dependence of the inhibition by cytochalasin B of antigen-initiated migration inhibition factor production.
The effects of cytochalasin B on the antigen recognition process have been examined using an in vitro assay of macrophage-inhibition factor (MIF) production by activated guinea pig T lymphocyte-enriched populations. The data indicate that cytochalasin B inhibits MIF production if added at the initiation of culture but not if added 2 hr after antigen. These data are interpreted as consistent with either an inhibition by cytochalasin B of the physical assoication of antigen-bearing marcrophage and lymphocytes or with an inhibition of the access of antigen to the T lymphocyte receptor.
D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D003571:Cytochalasin B / Q000494:pharmacology*; D004140:Dinitrophenols / Q000276:immunology; D005033:Ethylmaleimide / Q000494:pharmacology; D005971:Glutamates / Q000276:immunology; D006168:Guinea Pigs; D006854:Hydrocortisone / Q000494:pharmacology; D007271:Injections, Intradermal; D008239:Lysine / Q000276:immunology; D008263:Macrophage Migration-Inhibitory Factors / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D014373:Tuberculin
D000941:Antigens; D004140:Dinitrophenols; D005971:Glutamates; D008263:Macrophage Migration-Inhibitory Factors; D014373:Tuberculin; D003571:Cytochalasin B; D008239:Lysine; D005033:Ethylmaleimide; D006854:Hydrocortisone