Cell surface alterations induced by vaccinia and Newcastle disease viruses (38486).
A comparison was made of HEp-2 cell surface changes induced by NDV or vaccinia virus infection. Three parameters were examined as a function of time after infection: the kinetics of hemadosorption and the appearance of concanavalin (con A) binding sites, and alterations in electrophoretic mobility of single cells. The kinetics of appearance of con A binding sites was strikingly similar for both virus infections, whereas hemadsorption preceded NDA synthesis and followed vaccinia synthesis. These data suggest that in the vaccinia-infected cell the hemadsorption and con A binding sites are different. NDV infection or exposure of sham-infected cells to bacterial neuraminidase significantly reduced their anodal mobilities. This also occurred after enzyme treatment of vaccinia-infected cells. Measurements of the sialic acid content of NDV or sham-infected cells before and after neuraminidase treatment indicated the exposure to the enzyme or NDV materially reduced the sialic acid content of cells. Vaccinia-infected cells contained considerably more sialic acid than did normal cells. For  the vaccinia-infected cell a change in surface properties as detected by hemadsorption or increased con A binding was not reflected in a change in electrophoretic mobility.
D001665:Binding Sites; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002462:Cell Membrane; D003208:Concanavalin A / Q000378:metabolism*; D003584:Cytological Techniques; D004586:Electrophoresis; D006382:Hemadsorption; D007700:Kinetics; D007822:Laryngeal Neoplasms; D009439:Neuraminidase / Q000494:pharmacology; D009522:Newcastle disease virus; D012794:Sialic Acids / Q000032:analysis; D014616:Vaccinia virus; D014779:Virus Replication
D012794:Sialic Acids; D003208:Concanavalin A; D009439:Neuraminidase