Polynucleotides. XXVIII. Stimulation of the binding of aminoacyl-tRNA to ribosomes by tri- and polynucleotide analogs.
Messenger activity of synthetic tri- and polynucleotide analogs was studied by binding of 14C-labeled aminoacyl-tRNAs to ribosomes in the presence of the analogs. Synthetic messengers used were: poly(A) analogs in which adenosine was replaced by tubercidine (I), 3-deazaadenosine (II), 1-deazaadenosine (III) and 2-methyladenosine (IV); copolymers of adenosine and aristeromycin (V); cyclic triadenylate (VI); the heptanucleotide of 6,2'-O-cyclouridine (VII); the pentanucleotide of 8,2'-S-cycloadenosine (VIIIa); A-U-G analogs in which adenosine was replaced by 8,2'-O- and S-cycloadenosine (VIII), 8,5'-O- and S-cycloadenosine (IX); 8-oxyadenosine (x); 8-bromoadenosine (XI) and formycine (XII). Among these oligo- and polynucleotides, analogs which contained nucleotides of anti conformation having appropriate bases for Watson-Crick type hydrogen bonding stimulated the binding of corresponding tRNAs to ribosomes.
D000241:Adenosine / Q000031:analogs & derivatives; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008239:Lysine; D008715:Methionine; D009711:Nucleotides / Q000494:pharmacology*; D009841:Oligonucleotides / Q000494:pharmacology; D010649:Phenylalanine; D011119:Polynucleotides / Q000494:pharmacology; D012329:RNA, Bacterial / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014529:Uridine / Q000031:analogs & derivatives
D009711:Nucleotides; D009841:Oligonucleotides; D011119:Polynucleotides; D012329:RNA, Bacterial; D010649:Phenylalanine; D012343:RNA, Transfer; D008715:Methionine; D008239:Lysine; D000241:Adenosine; D014529:Uridine