In vitro immunogenicity of trinitrophenylated bacteriophage T4. I. Lack of helper cell cooperation.
A primary immune response was obtained in vitro to trinitrophenylated bacteriophate T4 (TNP-T4) by using normal BALB/c spleen cells challenged in vitro. The primary response was limited to anti-TNP; we failed to find evidence of a primary anti-T4 response in spleen cell cultures challenged with either TNP-T4 or native T4. In vivo priming with the carrier T4 failed to enhance the subsequent in vitro response to TNP-T4 although such priming sometimes caused suppression of the anti-hapten response. The addition of excess free carrier to spleen cell cultures challenged with TNP-T4 failed to suppress the anti-tnp response. Spleen cells treated with anti-theta plus complement were unaffected in their ability to respond to TNP-T4. We conclude that TNP-T4 should be classified among the thymus independent antigens.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000961:Antilymphocyte Serum / Q000494:pharmacology; D003090:Coliphages / Q000276:immunology*; D005260:Female; D005408:Flagellin / Q000008:administration & dosage; D006241:Haptens / Q000008:administration & dosage; D006433:Hemocyanins / Q000008:administration & dosage; D006462:Hemolytic Plaque Technique; D007074:Immunoglobulin G / Q000032:analysis; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D009578:Nitrobenzenes / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014302:Trinitrobenzenesulfonic Acid / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D000961:Antilymphocyte Serum; D006241:Haptens; D007074:Immunoglobulin G; D009578:Nitrobenzenes; D005408:Flagellin; D012710:Serum Albumin, Bovine; D014302:Trinitrobenzenesulfonic Acid; D006433:Hemocyanins