Accessibility of chromatin to DNA polymerase I and location of the F1 histone.
The effect of prebound poly-L-lysine upon the template activity of DNA, chromatin and F1 histone-depleted chromatin for E. coli DNA polymerase I has been investigated. Measurements have been made in the absence and presence of 0.1M NaCl. From the results we conclude that only ca 60% of the polylysine-accessible DNA, i.e. 22% of the total DNA of the chromatin, is accessible to the DNA polymerase I and that ca. 30% of the polylysine-accessible DNA, i.e. 11% of the total DNA, is associated with the F1 histone.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism*; D004247:DNA; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D008239:Lysine; D010455:Peptides / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D013698:Templates, Genetic; D013950:Thymus Gland
D002843:Chromatin; D006657:Histones; D010455:Peptides; D004247:DNA; D004254:DNA Nucleotidyltransferases; D012321:DNA-Directed RNA Polymerases; D008239:Lysine