The influence of mouse sera on colony formation and on the production of colony stimulating factor in vitro.
Results of studies on the in vitro production and release of colony stimulating factor (CSF), by bone associated cells cultivated in the presence or absence of mouse sera, are presented. Tissues from three strains of mice were shown to be equally effective in the production of CSF in vitro. Priming of the tissue donors by endotoxin 4 hours previously, or by irradiation 1, 7 or 15 days previously, significantly increased the CSF production. The addition of serum from normal mice to the culture medium had no effect on CSF production by tissue from normal mice but regularly inhibited CSF production by bones from irradiated animals. The inhibition was not due to the toxic effect of the sera on the CSF producing cells and was more pronounced in the cultures of NMRI than in the C57BL tissues. Some inhibition was also observed in cultures of tissue from endotoxin-primed NMRI mice. Extraction of the sera with ether removed the inhibitory material. The sera which inhibited the CSF production in vitro also inhibited the growth of granulocytic and macrophage colonies in soft agar cultures but did not inhibit the growth and DNA synthesis of cultured L-cells.
D000818:Animals; D001769:Blood; D001853:Bone Marrow / Q000276:immunology* / Q000378:metabolism / Q000528:radiation effects; D001854:Bone Marrow Cells; D002455:Cell Division; D002999:Clone Cells; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D006023:Glycoproteins / Q000096:biosynthesis*; D007739:L Cells / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D011830:Radiation Effects
D003115:Colony-Stimulating Factors; D003470:Culture Media; D006023:Glycoproteins; D004247:DNA