Characterization of ribonuclease NU cleavage sites in a bacteriophage phi80-induced ribonucleic acid.
Ribonuclease NU, an endoribonuclease isolated from human KB tissue culture cells, can cleave a bacteriophage phi80-induced RNA at four distinct sites. Nucleotide sequence analysis of the eight cleavage products has shown that the enzyme produces oligonucleotides terminating in 3'-phosphate groups, and that the four cleavage sites are in the only nonhydrogen-bonded region of the substrate. Various aspects of the cleavage reaction with this RNA and with other substrates are discussed.
D001483:Base Sequence; D002277:Carcinoma; D002460:Cell Line; D003090:Coliphages / Q000032:analysis*; D004267:DNA Viruses / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D004926:Escherichia coli; D006801:Humans; D009062:Mouth Neoplasms; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000032:analysis; D012367:RNA, Viral; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis
D009841:Oligonucleotides; D012367:RNA, Viral; D012265:Ribonucleotides; D004720:Endonucleases; D012260:Ribonucleases