Antifluorescein affinity columns. Isolation and immunocompetence of lymphocytes that bind fluoresceinated antigens in vivo or in vitro.
A new method for the isolation of specific immunocompetent lymphocytes has been described in which lymphocyte populations are exposed to fluoresceinated antigens (FLAGs) in vivo or in vitro, and the FLAG-binding cells retained on antifluorescein affinity columns. Specific cells are then eluted with an unrelated FL-labeled protein and shown to be fully immunocompetent. This methodology has been applied successfully in diverse antigenic systems including polymerized flagellin and TNP-specific B cells and alloantigen-reactive cytotoxic T lymphocytes. The method is rapid, inexpensive (requiring only antifluorescein beads), and can be applied to any antigens (or antibodies) in which a fluorescein group can be introduced.
D000818:Animals; D000941:Antigens; D001402:B-Lymphocytes / Q000032:analysis; D002469:Cell Separation / Q000379:methods; D002846:Chromatography, Affinity; D003601:Cytotoxicity Tests, Immunologic; D005452:Fluoresceins; D006241:Haptens; D008214:Lymphocytes / Q000032:analysis* / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000032:analysis
D000941:Antigens; D005452:Fluoresceins; D006241:Haptens