title: Chemical specificity of pyruvate kinase from yeast.
abstract: Three analogs of phosphoenolpyruvic acid: (Z)-phosphoenol-3-fluoropyruvate, (Z)-phosphoenol-3-bromopyruvate and (Z)-phosphoenol-alpha-ketobutyrate were found to be substrates for yeast pyruvate kinase (ATP: pyruvate (Z)-O-phosphotransferase, EC 2.7.1.40)with maximal velocities much greater than those found for rabbit muscle pyruvate kinase. The analogs exhibited sigmoidal kinetics, which become hyperbolic upon addition of the allosteric effector, fructose 1,6-diphosphate. Moreover, the reaction of (Z)-phosphoenol-3-bromopyruvate with ADP to produce bromopyruvic acid and ATP irreversibly inhibited the enzyme with a half-life of 32 min.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D001966:Bromine; D002087:Butyrates; D005461:Fluorine; D005636:Fructosephosphates / Q000494:pharmacology; D007700:Kinetics; D010728:Phosphoenolpyruvate / Q000031:analogs & derivatives / Q000494:pharmacology; D011770:Pyruvate Kinase / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D002087:Butyrates; D005636:Fructosephosphates; D005461:Fluorine; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D011770:Pyruvate Kinase; D001966:Bromine