Misincorporation of (TP during transcription of poly dAT-dAT and poly dABU-dABU.
The synthetic DNA alternating copolymers poly dAT-dAT and dABU-dABU have been transcribed with E. coli RNA polymerase to measure the level of BrdU-induced misincorporation of guanine during transcription. GTP is found to be misincorporated into both copolymers at a frequency of 1 per 1000-2000 nucleotides polymerized. Using alpha-32P-GTP, the nearest neighbors to GMP are found to be UMP (approximately 63%), GMP (approximately 25%) and AMP (approximately 17%), with no apparent difference between the two templates. These results suggest that BrdU-substitution in DNA does not necessarily increase the potential for base mispairing during transcription, and hence, promote the production of faulty RNA molecules.
D000227:Adenine Nucleotides; D001483:Base Sequence; D001973:Bromodeoxyuridine / Q000494:pharmacology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003839:Deoxyadenosines; D004926:Escherichia coli / Q000201:enzymology; D006160:Guanosine Triphosphate / Q000378:metabolism*; D007700:Kinetics; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D012329:RNA, Bacterial / Q000096:biosynthesis; D013698:Templates, Genetic; D013942:Thymine Nucleotides; D014158:Transcription, Genetic / Q000187:drug effects
D000227:Adenine Nucleotides; D003839:Deoxyadenosines; D011089:Polydeoxyribonucleotides; D012329:RNA, Bacterial; D013942:Thymine Nucleotides; D006160:Guanosine Triphosphate; D012321:DNA-Directed RNA Polymerases; D001973:Bromodeoxyuridine