Photo-incuced cross-linkage of gene-5 protein and bacteriophage fd DNA+.
The gene 5 protein, coded for by the bacteriophage fd, forms a complex with single stranded fd-DNA such that one gene 5 protein monomer interacts with four bases. Exposure of this complex to ultraviolet light results in the formation of covalent bonds between 25-30% of the gene 5 protein monomers which are bound to the DNA. In contrast, when the intact fd virion, which is a complex of coat protein and DNA, was exposed to ultraviolet irradiation, no detectable protein DNA cross-links were found.
D001426:Bacterial Proteins / Q000528:radiation effects*; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D003090:Coliphages; D004267:DNA Viruses; D004279:DNA, Viral / Q000528:radiation effects*; D003851:Deoxyribonucleases; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005796:Genes; D010179:Pancreas / Q000201:enzymology; D011485:Protein Binding; D011830:Radiation Effects; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004279:DNA, Viral; D003851:Deoxyribonucleases