Determination of alpha1-antitrypsin phenotypes by isoelectric focusing in polyacrylamide gels.
A method for alpha1-antitrypsin phenotyping is described that uses isoelectric focusing in a pH gradient 3.5 to 5 with polyacrylamide as supporting medium. The method is fast, reproducible, and reliable and offers some advantages over the currently used starch-gel electrophoresis.
D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D006801:Humans; D007223:Infant; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D010641:Phenotype; D000515:alpha 1-Antitrypsin / Q000032:analysis*
D000515:alpha 1-Antitrypsin