title: Antigen-initiated B lymphocyte differentiation. IV. The adherence properties of antibody-forming cell progenitors from primed and unprimed mice.
abstract: Distinct sub-populations of B lymphocytes may be fractionated by passage through glass-bead columns, due to a temperature-independent adherence of certain cells. The relative adherence properties of AFC-progenitors from spleens of primed or unprimed CBA mice were compared with an adoptive immune assay employing as antigen the hapten NIP on the carrier polymerized flagellin, under conditions where T lymphocytes did not limit the response. The AFC-progenitors for an anti-NIP IgM response from unprimed mice, whether conventional, germ-free, or athymic were more adherent than the majority of cells in the spleen. The AFC-progenitors for an IgG response from hapten-primed mice were less adherent that the majority of spleen cells. The results indicate that there are sub-classes of AFC-progenitors, differing in surface properties and probably corresponding to differences between the "antigen-inexperienced" and the "memory" B cell populations.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000032:analysis; D001402:B-Lymphocytes / Q000032:analysis*; D001426:Bacterial Proteins / Q000276:immunology*; D002448:Cell Adhesion / Q000187:drug effects; D005591:Chemical Fractionation; D005408:Flagellin / Q000276:immunology*; D006241:Haptens / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D009574:Nitro Compounds / Q000276:immunology; D010648:Phenylacetates / Q000276:immunology*; D011828:Radiation Chimera; D013154:Spleen / Q000276:immunology; D013997:Time Factors
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D006241:Haptens; D007075:Immunoglobulin M; D009574:Nitro Compounds; D010648:Phenylacetates; D005408:Flagellin