A defined medium for the growth of Clostridium tetani and other anaerobes of clinical interest.
The growth of six strains of Clostridium tetani was assessed in a chemically supplemented commercially available defined medium. All strains grew reliably even after 12 serial passages, and two strains produced demonstrable toxic activity after passage. Consistent growth of the test strains could also be obtained on a solid version of this medium ("CA109-S" medium), and the strains could be serially passaged on this medium. Preliminary evidence is presented that the medium supports the surface growth of some other test anaerobes. Such a defined solid medium might prove of value in further studies on the surface growth of C. tetani and of other anaerobes of clinical interest.
D000693:Anaerobiosis; D000818:Animals; D001419:Bacteria / Q000254:growth & development; D001439:Bacteroides / Q000254:growth & development; D003013:Clostridium / Q000254:growth & development; D003017:Clostridium tetani / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D005069:Evaluation Studies as Topic; D051379:Mice; D013744:Tetanus Toxin / Q000096:biosynthesis
D003470:Culture Media; D013744:Tetanus Toxin