Nucleotide sequence determination of bacteriophage T4 species I ribonucleic acid.
The nucleotide sequence of T4 species I RNA, one of several stable RNA's specifically coded for by bacteriophage T4, has been determined using 32-P-labeled material from T4-infected cultures of Escherichia coli. The purified RNA species which has been sequenced has been shown to hybridize well to T4 DNA (Wilson J.H., Kim, J.S., and Abelson, J.N. (1972) J. Mol. Biol. 71, 547-556). The sequence is: pCGAUUCGAGGAAAUAUCUUUGCCGUAAGCCGAGUAGCGUUUUUGACGGAACGUUCGGAUAUGGUUGAGAUAUGGCCUUUUAAAAUAUUGAGUAGCGUCAACUACUUAAUAACCGGGUUCGAAUCCCGGCGUUUCGU-CAA-OHACA-OH. Species I RNA which is 140 nucleotides long is also found to occur in shorter versions with 135 to 136 nucleotides which terminate with a 3'-phosphate. The molecule can be arranged in a secondary structure which shows some striking similarities to the classic cloverleaf pattern of a tRNA. The molecule is specifically cleaved by an E. coli nuclease into three segments by cleavage at a double-stranded region in the molecule. The function of species I RNA is unknown, but evidence presented elsewhere (Paddock, G.V., and Abelson, J. (1975) J. Biol. Chem. 250, 4207-4219) indicates that the gene for this RNA molecule has been preserved in evolution. The position of a mutation within species I RNA has been determined. This mutation results in incorrect processing of the RNA and lower relative yields of the RNA are present.
D000469:Alkaline Phosphatase; D001483:Base Sequence; D012831:Bombyx / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D003090:Coliphages / Q000032:analysis*; D004267:DNA Viruses / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D009690:Nucleic Acid Conformation; D010179:Pancreas / Q000201:enzymology; D012367:RNA, Viral / Q000302:isolation & purification; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis; D013045:Species Specificity
D012367:RNA, Viral; D012265:Ribonucleotides; D012260:Ribonucleases; D000469:Alkaline Phosphatase