[Obtaining specific non-adsorbed sera to Haemophilus influenza for the agglutination test on glass].
Type-specific agglutinating sera to capsular antigenas of H. influenzae were recovered (from 1:3200 to 1:25600). With the given immunization scheme there formed mostly IgG immunoglobulins, and only insignificant amount of IgM (separation of G-200 Sephadex gel). IgG were found to be responsible for the type-specificity of the sera to bacteria, whereas the cross reactions within the H. influenzae genus were connected connected with the IgM. Use of the sera heated at various temperature regimens offered a possibility of obtaining highly specific agglutinating sera with the aid of which express serodiagnosis of the isolated H. influenzae strains was possible.
D000327:Adsorption; D000372:Agglutination Tests; D000373:Agglutinins / Q000302:isolation & purification*; D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000918:Antibody Specificity; D001424:Bacterial Infections / Q000175:diagnosis*; D003429:Cross Reactions; D006193:Haemophilus influenzae / Q000276:immunology*; D006358:Hot Temperature; D007106:Immune Sera / Q000032:analysis; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification
D000373:Agglutinins; D000907:Antibodies, Bacterial; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Poluchenie spetsificheskikh neadsorbirovannykh syvorotok k Haemophilus influenzae dlia reaktsii aggliutinatsii na stekle