Qualitative and quantitative assay of trichothecin: a mycotoxin produced by Trichothecium roseum.
A method for quantitative determination of trichothecin in crude culture filtrates was presented. The method utilized an agar diffusion bioassay against Candida albicans, a colorimetric test involving a halochromatic reaction with sulfuric acid, and subsequent formation of blue color with methanol, and thin-layer chromatography of trichothecin and its dinitrophenylhydrazine derivative. A positive result in all three systems confirmed the presence of trichothecin. Quantitative results were generally in close agreement.
D000704:Analysis of Variance; D001681:Biological Assay; D002176:Candida albicans / Q000187:drug effects; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D005069:Evaluation Studies as Topic; D003904:Mitosporic Fungi / Q000378:metabolism; D012717:Sesquiterpenes / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis* / Q000096:biosynthesis / Q000494:pharmacology
D012717:Sesquiterpenes; D014255:Trichothecenes