In vitro biosynthesis of TSH- and LH-releasing factors by the human placenta.
Human chorionic membrane and human placental tissue contain substances with the same elution volume as hypothalamic TRF and LRF standards during separation by carboxyl-methyl-cellulose ion-exchange chromatography. Homogenates prepared from fresh human placentas delivered at term caused release of TSH (thyroid-stimulating hormone) and LH (luteinizing hormone) when bioassayed in rats. After dialysis of placental homogentes, synthesis of biologically active TSH-and LH-releasing factors can be shown to occur in vitro following the addition of appropriate precursor amino acids and cofactors.
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001681:Biological Assay; D002021:Buffers; D002266:Carboxymethylcellulose Sodium; D002823:Chorion / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D046508:Culture Techniques; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000096:biosynthesis; D006801:Humans; D007986:Luteinizing Hormone / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011392:Proline / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D013972:Thyrotropin / Q000378:metabolism*; D014316:Tritium
D000596:Amino Acids; D002021:Buffers; D014316:Tritium; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D013972:Thyrotropin; D011392:Proline; D002266:Carboxymethylcellulose Sodium