Non-specific and DNP--erythrocyte rosette formation after contact sensitization with DNCB.
DNA--guinea-pig erythrocyte rosette-forming cells (RFC), as found after contact sensitization of guinea-pigs with DNCB, were shown to be highly sensitive to pretreatment with cyclophosphamide or X-irradiation, which suggests that these cells are B cells. The finding was confirmed by rosette-blocking experiments using an anti-immunoglobulin serum. It has already been shown that they are not directly related to antibody production. Possibly they represent precursors of antibody-producing cells. An increase in rosette formation, using non-antigenically related erythrocytes, was shown to parallel development of cell-mediated immunity (CMI) in the lymph nodes. These non-specifically induced RFC were also found to be B cells. Data were obtained which make it improbable that either proliferation or influx of B cells during development of CMI plays a major role in this increase. An alternative mechanism is suggested, while a possible role for these non-specifically induced RC is proposed.
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology; D000961:Antilymphocyte Serum; D001402:B-Lymphocytes / Q000276:immunology*; D003520:Cyclophosphamide / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000276:immunology*; D004137:Dinitrochlorobenzene / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D006462:Hemolytic Plaque Technique; D007104:Immune Adherence Reaction; D007111:Immunity, Cellular; D007165:Immunosuppression Therapy; D008198:Lymph Nodes / Q000276:immunology; D009578:Nitrobenzenes / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D010081:Oxazolone / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes; D011135:Polysaccharides, Bacterial; D010081:Oxazolone; D003520:Cyclophosphamide; D010047:Ovalbumin