Synaptosomes containing large agranular vesicles isolated from developing rat brain.
Using the subcellular fractionation technique the fine structure of the isolated nerve endings (synaptosomes) from the hemispheres and brain stem of the 1-day old and adult rat was examined. In the synaptosomal fractions of the brain of 1-day old rats we observed a new type of nerve endings containing predominantly large agranular vesicles about 1,000 A in diameter. After incubation with alpha-methylnoradrenaline or 5-hydroxydopamine these vesicles remained agranular. It is assumed that the new type of large vesicles represent developing synaptic vesicles.
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development*; D001933:Brain Stem / Q000254:growth & development; D006892:Hydroxydopamines / Q000494:pharmacology; D008771:Nordefrin / Q000494:pharmacology; D051381:Rats; D013347:Subcellular Fractions; D013572:Synaptic Vesicles / Q000187:drug effects / Q000648:ultrastructure*; D013574:Synaptosomes / Q000648:ultrastructure*
D006892:Hydroxydopamines; D008771:Nordefrin