Quantitative assessment of thymus-dependent (t)-cell function in human peripheral blood.
A new micromethod for evaluating lymphocyte responses to phytohemagglutinin (PHA) has been developed and used for the survey of T-cell function in human peripheral blood. The results of lymphocyte responses to PHA stimulation in whole blood culture were expressed in terms of unit volume of blood (50 mul). This new method has eliminated the major uncontrollable factors of conventional methods, and appears to be more correlated with the cell-mediated immunity in vivo.
D000361:Agammaglobulinemia / Q000235:genetics; D000818:Animals; D000961:Antilymphocyte Serum; D002448:Cell Adhesion; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D003165:Complement System Proteins; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007104:Immune Adherence Reaction; D007153:Immunologic Deficiency Syndromes / Q000276:immunology; D007165:Immunosuppression Therapy; D016030:Kidney Transplantation; D037102:Lectins; D007945:Leukemia, Lymphoid / Q000276:immunology; D008213:Lymphocyte Activation; D011817:Rabbits / Q000276:immunology; D011947:Receptors, Antigen, B-Cell; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D000961:Antilymphocyte Serum; D037102:Lectins; D011947:Receptors, Antigen, B-Cell; D014316:Tritium; D003165:Complement System Proteins; D013936:Thymidine