Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells.
An antibody against purified chicken skeletal muscle tropomyosin is used in indirect immunofluorescence to visualize the localization of tropomyosin in a variety of nonmuscle cells. The antibody produces a fluorescent pattern which is very similar to that obtained with an actin-specific antibody. This pattern is composed of fluorescent fibers which are shown to be coincident with the fibers seen with phase-contrast optics. High resolution epifluorescent microscopy reveals that fibers stained with the actin antibody show a continuous fluorescence, while fibers reacted with the tropomyosin antibody show a periodic fluorescence. Measurements indicate that the lengths of the fluorescent segments are variable with an average of 1.2 mum while the spacing between segments is approximately 0.4 mum.
D000199:Actins / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002460:Cell Line; D002645:Chickens / Q000276:immunology; D002848:Chromatography, DEAE-Cellulose; D003550:Cystic Fibrosis; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology; D008856:Microscopy, Fluorescence; D010507:Periodicity; D011817:Rabbits / Q000276:immunology; D012867:Skin; D014335:Tropomyosin / Q000276:immunology / Q000302:isolation & purification*
D000199:Actins; D014335:Tropomyosin