Differential susceptibility of neonatal and adult murine spleen cells to in vitro induction of B-cell tolerance.
The relative susceptibility of neonatal and adult murine splenocytes to induction of B-cell tolerance was studied in vitro. Adult cells required approximately 1,000-fold more trinitrophenyl-human gamma globulin to be rendered tolerant than did cells from 9- to 12-day-old neonates. The potential effects of suppressor T cells were excluded by pretreating the cultured B cells with anti-Thy-1 and C' and the helper T cells with anti-Ly-2.2 and C'. The possible role of cell surface immunoglobulin isotypes in contributing to this observed difference is discussed.
D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D007108:Immune Tolerance; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D051379:Mice; D013154:Spleen / Q000276:immunology
D007072:Immunoglobulin D; D007075:Immunoglobulin M