Analysis of the Kinetics of the immune response by means of ionizing radiation.
The inhibitory kinetics of the primary immune response was studied by means of lethal irradiation. CBA mice were immunized with sheep red blood cells and 48, 72 and 96 hours later, they were irradiated with 750R. Afterwards, the number of direct plaque-forming cells was determined in their spleens during 58 to 66 hours at 4-hour intervals. After irradiation at 48 and 72 hours of the immune response, a plateau of different duration was obtained: 48 hours in the former and 24 hours in the latter case. In both cases, reduction in the PFC number began at about 96 hours of the resonse. The PFC reduction curves in these two series of experiments and those obtained 96 hours after irradiation and in control, non-irradiated mice were parallel. The mean half-time of the PFC number reduction as determined by these curves was 6 hours. The time of functioning of PFC in the spleen is discussed with respect to recruitment of proliferating precursors.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology* / Q000528:radiation effects; D002455:Cell Division; D005720:Gamma Rays; D006462:Hemolytic Plaque Technique; D007109:Immunity / Q000528:radiation effects; D007165:Immunosuppression Therapy; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology; D013997:Time Factors