Occurrence, transport, and storage of octopamine in human thrombocytes.
3H-octopamine was found to be accumulated in human platelets, achieving a maximum concentration gradient of 30:1. Its accumulation was partially inhibited by reserpine, imipramine, serotonin, ouabain, dinitrophenol, and iodoacetate. When octopamine was added to platelet preparations, it led to a decrease of both endogenous and 14C-serotonin. To determine whether octopamine accumulates in human platelets in vivo, preparations from 6 patients receiving monoamine oxidase inhibitors and 17 control subjects were assayed enzymatically for octopamine. Octopamine was detectable in all of the drug-treated patients, averaging 0.45 +/- 0.06 ng/mg protein, while only 4 of the 17 control subjects had detectable (greater than 0.05 ng/mg protein) platelet octopamine. Although much lower than platelet serotonin levels, these octopamine levels are in the range of those reported for platelet norepinephrine and epinephrine.
D001692:Biological Transport; D001792:Blood Platelets / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism; D004298:Dopamine / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D009638:Norepinephrine / Q000097:blood; D009655:Octopamine / Q000097:blood* / Q000494:pharmacology; D012701:Serotonin / Q000097:blood; D013997:Time Factors
D001798:Blood Proteins; D009655:Octopamine; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine