Beta-N-acetylhexosaminidase active on dermatan sulfate.
The dodecasaccharide obtained by treating dermatan sulfate with testicular hyaluronidase, chondroitinase AC, and beta-glucuronidase was incubated with diluted, normal human serum at pH 4.5 or 7.0 followed by chondro-4-sulfatase at pH 7.0. Analyses of the reaction products indicate release of hexosamine but not further degradation of the substrate. It is concluded that normal human serum possesses an exo-beta-N-acetylhexosaminidase active on dermatan sulfate.
D000818:Animals; D002807:Chondroitin / Q000031:analogs & derivatives*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003871:Dermatan Sulfate; D005417:Flavobacterium / Q000201:enzymology; D006026:Glycoside Hydrolases; D006596:Hexosaminidases / Q000097:blood*; D006801:Humans; D006821:Hyaluronoglucosaminidase; D008297:Male; D009844:Oligosaccharides; D011514:Proteus vulgaris / Q000201:enzymology; D012756:Sheep; D013737:Testis / Q000201:enzymology
D009844:Oligosaccharides; D003871:Dermatan Sulfate; D002807:Chondroitin; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; D006821:Hyaluronoglucosaminidase