title: T cell-dependent helper and suppressive influences in an adoptive IgG antibody response.
abstract: When immune spleen cells of mice immunized to a hapten carrier preparation 4 days previously were transferred to normal syngeneic hosts, they began to produce 7S antibody (presumably of the IgG class), provided that relatively small numbers of cells (about 1/10 spleen equivalent) were transferred. Increasing the number of transferred cells resulted in less IgG antibody formed. Depletion of the immune spleen cells of T cells by treatment with anti-theta serum and complement prevented IgG antibody formation. IgG antibody production by untreated and anti-therta serum-treated immune spleen cells could be enhanced (reinduced) by addition of small numbers of cells enriched for carrier-activated T cells. These suggest that T cells are necessary to stimulate antigen-activated B cells into IgG antibody production. Larger numbers of 'carrier-activated T cells' depressed IgG antibody production. Both enhancement and depression could be demonstrated to be antigen-specific. IgG antibody production high numbers of transferred immune spleen cells could be induced by treating the cells prior to transfer with suboptimal amounts of anti-theta serum and complement. It is argued that this results from the elimination of a T cell-dependent suppressor influence arising during a normal immune response.
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D002499:Centrifugation, Density Gradient; D006241:Haptens; D006386:Hemagglutination Tests; D006462:Hemolytic Plaque Technique; D007106:Immune Sera; D007114:Immunization; D007074:Immunoglobulin G; D007165:Immunosuppression Therapy; D008212:Lymphocyte Depletion; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013972:Thyrotropin; D014184:Transplantation, Homologous
chemical_list: D000888:Antibodies, Anti-Idiotypic; D006241:Haptens; D007106:Immune Sera; D007074:Immunoglobulin G; D013972:Thyrotropin