title: Synthesis of envelope polypeptides by Haemophilus influenzae during development of competence for genetic transformation.
abstract: Six polypeptides with apparent molecular weights of 95,000, 90,000, 80,000, 67,000, 64,000, and 43,000 were found to be characteristic of the cell envelopes of competent Haemophilus influenzae, and were synthesized entirely during the period of competence development. Two polypeptides with apparent molecular weights of 58,500 and 40,500 were synthesized during growth as well as during competence development, but were only associated with the envelope fraction of cells that had developed competence. The kinetics of synthesis of the competence-related envelope polypeptides showed a lag period of approximately 20 min. The observation of this lag period raises the question as to whether some of these competence-related polypeptides might be involved in the process of deoxyribonucleic acid uptake, since the development of this property also exhibits a sigmoid time course during competence development.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis*; D002473:Cell Wall / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D006193:Haemophilus influenzae / Q000254:growth & development / Q000378:metabolism*; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D014170:Transformation, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial