Fractionation of human T lymphocytes on wheat germ agglutinin-sepharose.
T cells from human peripheral blood was purified by fractionation on columns charged with human immunoglobulin and rabbit anti-human immuno-globulin. When assayed with 125I- or fluorescein isothiocyanate-labeled wheat-germ agglutinin (WGA), a weakly binding and a strongly binding subpopulation could be distinguished. These T-cell subpopulations were fractionated on columns charged with WGA, convalently bound to Sepharose 6MB. The cells responding to the mitogens leukoagglutinin from Phaseolus vulgaris and concanavalin A were enriched in the strongly binding subpopulation (approximately 20% of the T cells) while they were depleted from the weakly binding subpopulation.
D001665:Binding Sites; D001773:Blood Cells; D002469:Cell Separation; D003208:Concanavalin A; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D037121:Plant Lectins; D013601:T-Lymphocytes / Q000378:metabolism*; D014908:Triticum
D037102:Lectins; D037121:Plant Lectins; D003208:Concanavalin A