Human T-lymphocyte rosette formation: inhibition by cytochalasin B.
The effects of cytochalasin B, colchicine and vinblastine on the rosette formation of human T lymphocytes with neuraminidase-treated human erythrocytes (nHRBC) and with sheep red blood cells (SRBC) have been studied. Pretreatment of the lymphocytes with cytochalasin B which affects microfilament action reversibly inhibits both nHRBC and SRBC rosette formation. Colchicine and vinblastine known to interact with microtubules causes no major reduction in rosette-forming cells. The results suggest that normally functioning microfilaments are necessary for nHRBC and SRBC rosetting, whereas microtubules are not essential for the blinding of the erythrocytes to the lymphocytes.
D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology*; D006801:Humans; D007104:Immune Adherence Reaction; D013601:T-Lymphocytes / Q000187:drug effects*; D014747:Vinblastine / Q000494:pharmacology
D003571:Cytochalasin B; D014747:Vinblastine; D003078:Colchicine