Genetic analysis of lymphocyte activation by lipopolysaccharide Endotoxin.
A genetic study of in vitro lymphocyte blastogenesis by the B-cell mitogen lipopolysaccharide endotoxin has been performed, using both low-responder C3H/HeJ mice and high-responder CBA/J mice. The crossbreeding of these strains produced F1 progeny that were intermediate in responsiveness. Responder types from the backcross of the F1 to the C3H/HeJ strain segregated into intermediate- and low-responder phenotypes, whereas intermediate- and high-responder phenotypes were produced from the backcross of the F1 to the CBA/J strain. The F2 generation consisted of all three responder phenotypes in frequencies that fit the classical Mendelian ratio of 1:2:1, indicating that the mitogenic response to lipopolysaccharide endotoxin in mice is most likely governed by a pair of autosomal co-dominant genes.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D003433:Crosses, Genetic; D005260:Female; D005796:Genes; D005799:Genes, Dominant; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013045:Species Specificity
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial