Determination of OH 5 tryptophan by chromato-spectrofluorimetry in carcinoid tumors.
We have suggested the determination of different indolic metabolites of tryptophan: OH 5 trptophan (5 HTP), OH 5 tryptamine (5 HT), OH 5 indolyl-acetic acid (5 HIA) by column chromatography on cationic coupled with spectrofluorimetry after amplification obtained with o-phtaldehyde. The different metabolites are determined alone or simultaneously depending on the buffer used. The following qualitates are examined: sensitivity, specificity, accuracy. We have established normal reference values. For 5 HTP the normal values in urine are less than or equal to 50 microgram per day. In 12 cases of carcinoid tumors we have measured 5 HIA, 5 HTP, 5 HT and calculated the ratio between 5 HT and 5 HTP in the urine which makes it possible to have an idea of the enzyme activity in the hydroxyindol pathway in cases of intestinal and bronkial carcinoids.
D006916:5-Hydroxytryptophan / Q000652:urine*; D002277:Carcinoma / Q000097:blood / Q000652:urine*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine; D012701:Serotonin / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence / Q000379:methods; D014364:Tryptophan / Q000378:metabolism
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D014364:Tryptophan; D006916:5-Hydroxytryptophan