[Automation of extraction in the determination of blood triglyceride].
A complete automation of the determination of triglycerides is described which replaces the manual extraction and the final determination with an autoanalyzer chain, based on the method of Royer et al. For the automation of triglyceride extraction, we have modified the sampler, by incorporating a magnetic stirrer under the sample table. The sample table has two rows of holes. The sera, which are taken from the inner row and diluted with sulphuric acid, are reacted quantitatively with the extraction reagent in the beakers of the outer row, which contain a magnetic follower. After stirring, the upper phase is quantitatively removed and the determination is started. The problem of phasing the individual steps of the analysis is thus solved.
D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods*; D003124:Colorimetry; D006801:Humans; D013464:Sulfuric Acids; D014280:Triglycerides / Q000097:blood* / Q000302:isolation & purification
D013464:Sulfuric Acids; D014280:Triglycerides
Automatisation de l'extraction dans le dosage des triglycérides du sérum