Release of (oligo) peptidyl-tRNA from ribosomes by erythromycin A.
Erythromycin A released peptidyl-tRNA in the in vitro polypeptide synthesis system with bacterial components programmed by synthetic polynucleotide. This is consistent with our hypothesis that erythromycin A inhibits translocation by preventing proper situation of oligopeptidyl-tRNA in the donor (D) site on ribosomes.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis; D002499:Centrifugation, Density Gradient; D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D008239:Lysine; D009239:N-Formylmethionine / Q000378:metabolism; D009842:Oligopeptides; D014176:Protein Biosynthesis / Q000187:drug effects; D012329:RNA, Bacterial / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014633:Valine / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D009842:Oligopeptides; D012329:RNA, Bacterial; D009239:N-Formylmethionine; D004917:Erythromycin; D012343:RNA, Transfer; D014633:Valine; D008239:Lysine