The importance of Escherichia coli ribosomal proteins L1, L11 and L16 for the association of ribosomal subunits and the formation of the 70-S initiation complex.
50-S subunits were washed with LiCl solutions of different concentrations. After washing with 1 M LiCl solution the particles lost their ability to attach either to 30-S subunits or to the AUG - 30-S subunit - fMet-tRNAfMet complex or to a poly(U) - 30-S subunit - Phe-tRNAPhe complex. Those proteins which were removed by LiCl were fractionated on a Sephadex G-100 column. Of the fractionated proteins only the combinations L1 and L11 or L1 and L16 were essential for the association of 50-S 1.0 cores (particles prepared by washing 50-S subunits in 1.0 M LiCl) with 30-S subunits. These three proteins were also required for the formation of a stable complex between 50-S 1.0 cores, mRNA, 30-S subunits and aminoacyl-tRNA.
D001665:Binding Sites; D002712:Chlorides / Q000494:pharmacology; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000378:metabolism*; D008094:Lithium / Q000494:pharmacology; D008715:Methionine; D008970:Molecular Weight; D010442:Peptide Chain Initiation, Translational; D010649:Phenylalanine; D011072:Poly U / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D012343:RNA, Transfer / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D046249:Transfer RNA Aminoacylation
D002712:Chlorides; D012269:Ribosomal Proteins; D011072:Poly U; D010649:Phenylalanine; D011691:Puromycin; D012343:RNA, Transfer; D008094:Lithium; D008715:Methionine