Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry.
Out of a total of eleven bifunctional reagents tested as fixatives for light microscopic immunohistochemistry, four were found satisfactory when applied in the vapour phase to freeze-dried blocks. These were diethylpyrocarbonate, as observed in earlier studies, dimethyladipimidate, p-benzoquinone, and diacetyl. Adequate but less satisfactory liquid-phase fixation was provided by three reagents (dimethyladipimidate, dimethylsuberimidate, p-benzoquinone). None of the eleven reagents gave optimal preservation of antigencity and structure when tested at the ultrastructural level. Encouraging results were obtained with p-benzoquinone, however.
D000818:Animals; D004285:Dogs; D004386:Duodenum / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005612:Freeze Drying; D006651:Histocytochemistry / Q000379:methods*; D007082:Ileum / Q000648:ultrastructure; D007158:Immunologic Techniques / Q000379:methods*; D007202:Indicators and Reagents; D007421:Intestine, Small / Q000648:ultrastructure*; D051379:Mice; D051381:Rats; D013045:Species Specificity; D013552:Swine
D007202:Indicators and Reagents