Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis.
The enzyme-linked immunosorbent assay (ELISA) technique, using an ultrasonicate of Treponema pallidum as antigen, has been evaluated as a serological test for syphilis. It is concluded that the test is simple, reliable, and relatively quick and that its sensitivity in all stages of syphilis is equal to the FTAABS test. Because its specificity is probably also high, ELISA might be used in future as a first-line screening test for the serodiagnosis of syphilis.
D000327:Adsorption; D000469:Alkaline Phosphatase; D000888:Antibodies, Anti-Idiotypic; D003168:Complement Fixation Tests; D005455:Fluorescent Antibody Technique; D006801:Humans; D007136:Immunoglobulins; D013588:Syphilis Serodiagnosis / Q000379:methods*; D014209:Treponema Immobilization Test
D000888:Antibodies, Anti-Idiotypic; D007136:Immunoglobulins; D000469:Alkaline Phosphatase