Differential regulatory effects of accessory cells on the generation of cell-mediated immune reactions.
Conditions for the in vitro generation of cell-mediated immunity to H-2 and non-H-2 alloantigens by mouse spleen cells were compared to conditions for generation of in vitro humoral immunity to sheep red cells. Normal spleen cells grown in standard or allogeneic cell-conditioned media developed strong cellular responses to H-2 antigens but little or no response to non-H-2 antigens. Subpopulations of spleen cells from which the adherent cells had been removed generated strong cellular responses to both H-2 and non-H-2 antigens, provided the cells were cultured in conditioned media. Both cellular responses were shown to be mediated by T cells. Accessory cells from the peritoneum and/or spleen differentially inhibited the non H-2 cellular responses at doses which did not inhibit humoral responses.
D000818:Animals; D000917:Antibody Formation; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003470:Culture Media; D003601:Cytotoxicity Tests, Immunologic; D004912:Erythrocytes / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D006649:Histocompatibility Antigens; D007111:Immunity, Cellular; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology
D002860:Chromium Radioisotopes; D003470:Culture Media; D006649:Histocompatibility Antigens