Genetic regulation of the antibody response to H-2Db alloantigens in mice. I. Differences in activation of helper T cells in C57BL/10 and BALB/c congenic strains.
B10.A(5R) mice immunized with C57BL/10 spleen cells demonstrate a normal T-cell-mediated cytotoxicity to H-2Db tumor cells but they do not mount any IgG antibody response to H-2Db alloantigens. B10.A(5R) mice do show a high titered IgG response when immunized with A.BY cells, which differ at H-2Db plus non-H-2 cell surface antigens, or with B10.A(2R) cells, which differ at H-2Db, H-2Kk, and H-2Ik cell surface antigens. These findings indicate a failure of the T-helper cells to induce the switch from IgM to IgG when the H-2Db alloantigens are the only difference on the immunizing cell. In immunizing H-2d mice with congenic H-g2 cells which differ only in the H-2Db region, mice of the C57BL/10 background made only IgM antibodies whereas mice of the BALB/c background made IgG antibodies. This comparison confirms that genes separate from H-2 regulate the T-cell helper function. The genes that influence the T-cell helper function do not regulate the T-cell-mediated cytotoxicity.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D005809:Genes, Regulator; D006238:Haploidy; D006649:Histocompatibility Antigens; D007111:Immunity, Cellular; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007938:Leukemia / Q000276:immunology / Q000473:pathology; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D006649:Histocompatibility Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003165:Complement System Proteins