[Basic factors in the cerebellar Purkinje cell polyploidization in chicken embyogeny. II. The changes in the content and concentration of proteins, protein SH-groups and RNA in the cytoplasm of Purkinje cells in the process of their differentiation and specialization].
Cytophotometric study of the cerebellum Purkinje cells in the chick embryos(10th-21st day) showed that the total amount of protein increased at this period proportionately to the growth of cells, its concentration remaining relatively constant. Thebasic and acid proteins ratio changed, the acid proteins increasing. Content of the protein SH-group increases considerably during the tigroid and eneurofibrillas formation, - simultaneously with the conent of RNA. The periods of intensification of the protein synthesis coincide with the morpho-functional maturation of neurons and formation of signs characterizing the maturity of the embryo as a whole. Supposedly the changesin the activity of nuclear apparatus of the cells, manifested in the increase of nuclear size and the number and size of nucleoli, underlie this process.
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D002642:Chick Embryo; D003584:Cytological Techniques; D003593:Cytoplasm / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology; D004625:Embryo, Nonmammalian; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011123:Polyploidy; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D011689:Purkinje Cells / Q000166:cytology* / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013997:Time Factors
D011506:Proteins; D013438:Sulfhydryl Compounds; D012313:RNA
Osnovnye faktory poliploidizatsii kletok Purkin'e mozzhechka v émbriogenezekur. II. Izemeneniia v soderzhanii i kontsentratsii belkov, belkovykh SH-grupp iRNK v tsitoplazme kletok Purkin'e v protsesse ikh differentsirovki i spetsializatsii