Mechanism of B-cell activation and paralysis by thymus-independent antigens. Additive effects between NNP-LPS and LPS in the specific response to the hapten.
Normal spleen cells showed a bell-shaped dose response profile when stimulated in vitro with the thymus-independent antigen (4-hydroxy-3,5-dinitrophenyl)acetyl (NNP)-lipopolysaccharide (LPS) with regard to the development of high-avidity plaque-forming cells to NNP. The addition of suboptimal concentrations of LPS to cultures stimulated by suboptimal concentrations of NNP-LPS resulted in optimal induction of B cells in that affinity fraction. Addition of LPS to cultures optimally stimulated by NNP-LPS resulted in paralysis of the specific cells. These results are interpreted in terms of the additive effects between the mitogenicity of LPS and the mitogenicity of NNP-LPS, the latter being selectively focused on the specific cells, thus providing further evidence for the 'one nonspecific signal' hypothesis for immune activation of B cells.
D000276:Adjuvants, Immunologic; D000818:Animals; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D004140:Dinitrophenols; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli; D005260:Female; D006241:Haptens; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013154:Spleen / Q000276:immunology
D000276:Adjuvants, Immunologic; D000941:Antigens; D004140:Dinitrophenols; D006241:Haptens; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial