Separation of the glycopeptides released by the action of rennin on whole milk.
A comparison has been made of a number of different elution conditions for the separation by ion-exchange chromatography of the glycopeptides released by the action of rennin on whole milk. It was found that the most satisfactory separation was achieved using an NaCl gradient (0.10-0.18 M) in 0.049 M-KH2PO4+0.001 M-Na2HPO4 at a constant pH of 5.1.
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D012085:Chymosin / Q000378:metabolism*; D006020:Glycopeptides / Q000302:isolation & purification* / Q000378:metabolism; D008892:Milk / Q000032:analysis
D006020:Glycopeptides; D012085:Chymosin