Gas chromatographic determination and pharmacokinetics of 4-hydroxybutrate in dog and mouse.
A rapid and reproducible method was developed to extract 4-hydroxybutyrate from plasma as 4-butyrolactone for subsequent gas chromatographic (GLC) assay. The drug, an intravenous anesthetic and oral hypnotic in man, was infused into four dogs and the plasma concentration was determined by 14C-isotope dilution and GLC. Pharmacokinetic parameters for distribution and elimination were calculated. A capacity-limited process appears to be involved in the elimination fo 4-hydroxybutyrate in the dog. Macroautoradiogrpahy recieved the distribution pattern in normal and pregnant adult mice.
D000818:Animals; D001345:Autoradiography; D002849:Chromatography, Gas; D004285:Dogs; D005260:Female; D006885:Hydroxybutyrates / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D007263:Infusions, Parenteral; D007700:Kinetics; D008297:Male; D008722:Methods; D051379:Mice; D011247:Pregnancy; D011865:Radioisotope Dilution Technique
D006885:Hydroxybutyrates