A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization.
Cytoplasm of maturing amphibian oocytes possesses a factor that induces germinal vesicle breakdown. This factor was extracted from Rana pipiens eggs and assayed by microinjection in Xenopus laevis oocytes. The activity of this factor is Mg-dependent, Ca-sensitive, and associated with heat-labile protein. Centrifugation on a sucrose density gradient revealed that the factor exists in three different molecular sizes.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003593:Cytoplasm / Q000502:physiology; D004527:Egg Proteins / Q000502:physiology; D005260:Female; D008274:Magnesium / Q000494:pharmacology; D008540:Meiosis; D008970:Molecular Weight; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure; D009866:Oogenesis; D010063:Ovum / Q000502:physiology*; D011894:Rana pipiens
D004527:Egg Proteins; D008274:Magnesium; D002118:Calcium