The metabolism of N4-hydroxycytidine-a mutagen for Salmonella typhimurium.
Salmonella typhimurium cells were grown in the presence of (14C)-N4-hydroxycytidine (N4OHcyd), a mutagenic nucleoside, and labelling in DNA and RNA digest was traced. The results show that this analogue is incorporated into RNA at a level of 40 mug/100 mg, and into DNA with a yield at least 100 times smaller. Some N-minus4OHcyd was rapidly metabolized and labelling was found in all ribo- and deoxyribo-nucleosides.
D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D003562:Cytidine / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D003851:Deoxyribonucleases; D009153:Mutagens / Q000378:metabolism*; D009705:Nucleosides / Q000032:analysis / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000378:metabolism; D012260:Ribonucleases; D012486:Salmonella typhimurium / Q000032:analysis / Q000378:metabolism*
D004269:DNA, Bacterial; D009153:Mutagens; D009705:Nucleosides; D012329:RNA, Bacterial; D003562:Cytidine; D003851:Deoxyribonucleases; D012260:Ribonucleases