Overproduction of lysine by mutant strains of Escherichia coli with defective lysine transport systems.
Mutants selected on the basis of their resistance to S-(beta-aminoethyl) cysteine and overproduction of lysine were found to be defective in the lysine transport system. The overproduction of lysine was not due to mutation affecting either of the two regulatory enzymes aspartokinase and dihydrodipicolinic acid synthetase. Uptake of labeled lysine by the lysine-specific transport system was reduced to a negligible level, while uptake by the lysine, ornithine, arginine system was also affected. A hypothesis regarding the nature of these mutations and their effects on the regulation of lysine biosynthesis is discussed.
D001120:Arginine / Q000378:metabolism; D001222:Aspartate Kinase / Q000378:metabolism; D001693:Biological Transport, Active; D002250:Carbon Radioisotopes; D003470:Culture Media; D003545:Cysteine / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005809:Genes, Regulator; D006715:Homoserine Dehydrogenase / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism; D008239:Lysine / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000494:pharmacology; D009154:Mutation; D009952:Ornithine / Q000378:metabolism; D010848:Picolinic Acids
D002250:Carbon Radioisotopes; D003470:Culture Media; D010848:Picolinic Acids; D001120:Arginine; D009952:Ornithine; D006715:Homoserine Dehydrogenase; D001222:Aspartate Kinase; D006836:Hydro-Lyases; D008239:Lysine; D003545:Cysteine