title: Immunoglobulin turnover in B lymphocyte subpopulations.
abstract: "In vitro" turnover of leucine-labeled and of radioiodinated IgM has been studied with cells from various lymphoid organs of nude mice, i.e. lymph nodes, thoracic duct, spleen and bone marrow, as well as with subpopulations of B cells from spleen and bone marrow separated by free flow electrophoresis. Three types of IgM-producing lymphocytes could be distinguished by their turnover rates of IgM, by the size of the released IgM and by the capacity of the IgM molecules to be labeled by the lactoperoxidase-catalyzed radioiodination reaction and/or by incorporation of radioactive leucine. Type I cells release 7-8 S IgM rapidly (t1/2 = 1-3h); the released IGM is leucine-labeled and radioiodinated. Type II cells release 7-8 S IgM slowly (t1/2 =10-30); the released IgM is leucine labeled and radioiodinated. Type III cells release 19 S IgM rapidly (t1/2 =2-4 h); the released IgM is leucine labeled, but not radioiodinated. Lymph nodes and thoracic duct contain predominantly type II cells, bone marrow contains type I and II cells, spleen contains type I,II and III cells. It is suggested that type III cells are Ig-secreting plaque-forming plasma cells, type II cells are small, resting "memory" B cells, and type I cells may be newly formed antigen-inexperienced B cells.
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology / Q000502:physiology; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002469:Cell Separation; D007075:Immunoglobulin M / Q000378:metabolism*; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D008970:Molecular Weight; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*; D013154:Spleen / Q000276:immunology / Q000502:physiology
chemical_list: D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell