Human melanoma FUHS-1. Description of a new cell line.
Presented is a new established cell line, which was cultivated from a melanoma originating from a melanosis praeblastomatosa circumscripta of the leg. Recently subculture No. 105 was reached after a period of 31 month. Medium of primary culture was TC Medium Eagle spinner modified, followed from subculture No. 1 by MEM/Hanks/0.35 g/l NaHCO3. From subculture No. 6 NCTC 135 was applied simultaneously-each respective supplements. The cells grow as monolayer; absolute plating efficiency of the established cell line lies by 800/0; epithelial-like and dendritic melanin-producing cells are prominent. The number of chromosomes is considerably fluctuating; the karyotype of the established cell line lies within the range of subtriploidy. Sizewise a marker in submedian position of centromer is to be compared with one of group.
D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques; D003584:Cytological Techniques; D005260:Female; D006801:Humans; D007621:Karyotyping; D007866:Leg; D008543:Melanins / Q000096:biosynthesis; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D011230:Precancerous Conditions; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology*
D003470:Culture Media; D008543:Melanins
Human-Melanom FUHS-1. Beschreibung einer neuen Dauerzellinie