Amino acid synthetic media for fungal pathogens based on aminopeptidase specificities: Histoplasma capsulatum, Blastomyces dermititidis, Paracoccidioides brasiliensis and Cryptococcus neoformans.
The development of simple and chemically defined liquid media for Histoplasma capsulatum, Blastomyces dermatitidis, Paracoccidioides brasiliensis and Crypto-occus neoformans according to their aminopeptidases profiles as amino acid requirement was described. When 1.5% purified agar was added, these media also supported excellent mycelial growth and sporulation of the deep mycoses. H. capsulatum was converted to and maintained in yeast phase when 0.1% L-cystine was added to the solid medium incubated at 37 degrees C.
D000596:Amino Acids / Q000378:metabolism*; D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001758:Blastomyces / Q000201:enzymology / Q000254:growth & development*; D003454:Cryptococcus / Q000254:growth & development*; D003455:Cryptococcus neoformans / Q000201:enzymology / Q000254:growth & development*; D003470:Culture Media; D003553:Cystine / Q000378:metabolism; D005658:Fungi / Q000254:growth & development*; D006658:Histoplasma / Q000201:enzymology / Q000254:growth & development*; D006801:Humans; D051379:Mice; D010228:Paracoccidioides / Q000201:enzymology / Q000254:growth & development*
D000596:Amino Acids; D003470:Culture Media; D003553:Cystine; D000626:Aminopeptidases