Endotoxin binding by charged and uncharged resins.
Cholestyramine (Dowex 1-X2), a strongly basic anion-exchange resin, has previously been shown to bind bacterial endotoxin, preventing both its toxicity and intestinal absorption. Because hemoperfusion through charged and uncharged resins is practical, a study was undertaken to test the endotoxin-binding characteristics of a number of resins. The resin to be tested was washed and swelled overnight, and 1 mg/ml of 51Cr-labeled endotoxin was added and the mixture, agitated and incubated at 37 degrees for a specific time period. In the Dowex 1 series, the 1-X2 was superior to the 1-X4 and 1-X8 in its ability to bind E. coli endotoxin, removing about 90% from solution in 15 min. Increasing mesh size seemed to offer more binding sites for each Dowex 1 resin. Activated charcoal adsorbed about 90% of the endotoxin also, but Amberlite XAD-2 showed little binding capacity. Injection of filtrate from unlabeled E. coli and S. typhosa resin-treated solution into rats, demonstrated that both Dowex 1-X2 and activated charcoal prevented the transaminase rise noted in animals injected with solutions not so treated. It is concluded that Dowex 1-X2 resin and activated charcoal efficiently remove endotoxin in vitro, and may offer a unique method for removing circulating endotoxin in vivo.
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002606:Charcoal / Q000032:analysis; D004731:Endotoxins / Q000032:analysis; D004926:Escherichia coli; D005260:Female; D008070:Lipopolysaccharides; D008968:Molecular Conformation; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial; D051381:Rats; D012117:Resins, Synthetic / Q000494:pharmacology; D012485:Salmonella typhi; D013997:Time Factors
D004731:Endotoxins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D012117:Resins, Synthetic; D002606:Charcoal; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase