A simple method for the preparation of large quantities of pure plasmid DNA.
Polyethylene glycol quantitatively precipitates plasmid DNA of molecular weight 6-123-10-6, from cleared lysates of plasmid-carrying bacterial strains, After resuspension and density-gradient centrifugation of the precipitated DNA, it is unchanged in length and in transformation efficiency for Escherichia coli K12. Plasmid DNA can be easily prepared in large quantities by including a polyethylene glycol precipitation step in standard plasmid isolation procedures.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000032:analysis* / Q000187:drug effects; D005069:Evaluation Studies as Topic; D005111:Extrachromosomal Inheritance; D008722:Methods; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D011092:Polyethylene Glycols; D013501:Surface-Active Agents; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D013501:Surface-Active Agents; D011092:Polyethylene Glycols