Storage and metabolism of poly(adenylic acid)-mRNA in germinating cotton seeds.
When the RNA from both the nucleus and polysomes of germinated cotton seeds were fractionated on poly(U)-cellulose columns it was found that both contained poly(A)-RNA. In ungerminated seeds most of the poly(A)-RNA is located in the nucleus of the cell. 3'-Deoxyadenosine was found to inhibit the synthesis of both nuclear and polysomal poly(A) by 75% in germinated seeds, but had little or no effect on protein synthesis during the first 6 hr of germination. After this time 3'-deoxyadenosine had a pronounced inhibitory effect on protein synthesis presumably due to the inhibition of poly(A) addition to newly synthesized mRNA.
D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002846:Chromatography, Affinity; D003839:Deoxyadenosines / Q000494:pharmacology; D003368:Gossypium; D010940:Plant Proteins / Q000096:biosynthesis; D011061:Poly A / Q000302:isolation & purification / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*
D003839:Deoxyadenosines; D010940:Plant Proteins; D012333:RNA, Messenger; D011061:Poly A