Antagonism of B lymphocyte mitogenesis by anti-immunoglobulin antibody.
Anti-mouse-immunoglobulin-antibodies (AMIg) were shown to suppress mitogenesis in B lymphocytes that were cultured with lipopolysaccharide, (LPS), or polymerized-flagellin, (POL). Preincubation of spleen cells with AMIg plus LPS has a greater suppressive effect on a subsequent mitogenic response to LPS than did preincubation with AMIg alone. Preincubation with LPS alone had, if anything, a slight enhancing effect. These experiments suggested that AMIg did not suppress mitogenesis by interference with the initial binding of the mitogen, but rather exerted a separated negative effect that occurred subsequent to, or in the process of, B cell-stimulation.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D004926:Escherichia coli / Q000276:immunology; D005408:Flagellin; D006041:Goats / Q000276:immunology; D007065:Idoxuridine / Q000378:metabolism; D007106:Immune Sera; D007136:Immunoglobulins; D007165:Immunosuppression Therapy; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D008938:Mitosis; D011135:Polysaccharides, Bacterial; D012475:Salmonella / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D000888:Antibodies, Anti-Idiotypic; D007106:Immune Sera; D007136:Immunoglobulins; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D014316:Tritium; D005408:Flagellin; D007065:Idoxuridine; D013936:Thymidine