Lipophilic chelator inhibition of electron transport in Escherichia coli.
The lipophilic chelator bathophenanthroline inhibits electron transport in membranes from Escherichia coli. The less lipophilic 1,10-phenanthroline, bathophenanthroline sulfonate, and alpha,alpha-dipyridyl have little effect. Reduced nicotinamide adenine dinucleotide oxidase is more sensitive to bathophenanthroline inhibition than lactate oxidase activity. Evidence for two sites of inhibition comes from the fact that both reduced nicotinamide adenine dinucleotide menadione reductase and duroquinol oxidase activities are inhibited. Addition of uncouplers of phosphorylation before bathophenanthroline protects against inhibition.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology*; D003864:Depression, Chemical; D004579:Electron Transport / Q000187:drug effects; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D006873:Hydroquinones / Q000378:metabolism; D007773:Lactates; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010618:Phenanthrolines / Q000494:pharmacology*; D011808:Quinone Reductases / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology; D014812:Vitamin K / Q000378:metabolism
D002614:Chelating Agents; D006873:Hydroquinones; D007773:Lactates; D010618:Phenanthrolines; D014475:Uncoupling Agents; D014812:Vitamin K; D000429:Alcohol Oxidoreductases; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases