Cytotoxic activity of purified guinea pig lymphotoxin against various cell lines.
Cytotoxic activity of the purified guinea pig lymphotoxin (GLT) was tested on various blood cells and various cultured cell lines. GLT was generally ineffective on erythrocytes and lymphocytes, but it was somewhat effective on peritoneal exudate cells indicating GLT might play an important role in the regulatory mechanism of the cellular immunity. Considerable difference in target cell sensitivity was observed among the cultured cell lines tested. The sensitivity of L cells to GLT was significantly lowered by the trypsin treatment of the cells. Furthermore, porcine thyroglobulin glycopeptide exerted weak but definite inhibitory activity against GLT. These results suggest that the cytotoxicity of GLT might be triggered by the binding of GLT to the receptor sites of glycoprotein nature on the cell surface.
D000818:Animals; D001667:Binding, Competitive; D050260:Carbohydrate Metabolism; D002460:Cell Line; D004912:Erythrocytes / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism; D006168:Guinea Pigs; D008214:Lymphocytes / Q000187:drug effects; D008233:Lymphotoxin-alpha / Q000633:toxicity*; D008565:Membrane Proteins / Q000378:metabolism; D011955:Receptors, Drug / Q000378:metabolism; D014357:Trypsin
D006023:Glycoproteins; D008233:Lymphotoxin-alpha; D008565:Membrane Proteins; D011955:Receptors, Drug; D014357:Trypsin