Simultaneous autoradiographic and immunohistochemical localization of estrogen and gonadotropin in the rat pituitary.
A combined autoradiographic and immunohistochemical technique is introduced which is suitable to simultaneously localize hypophyseal sites of nuclear (3-H)-estradiol uptake and anti-hCG-characterized "gonadotropes". Most, but not all, "gonadotropes" show significant nuclear concentration of radioactivity. A large percentage of non-"gonadotropes" also show nuclear uptake of label.
D000818:Animals; D001345:Autoradiography; D004967:Estrogens / Q000302:isolation & purification*; D006065:Gonadotropins, Pituitary / Q000302:isolation & purification*; D006651:Histocytochemistry; D007158:Immunologic Techniques; D010902:Pituitary Gland / Q000032:analysis*; D051381:Rats; D014316:Tritium
D004967:Estrogens; D006065:Gonadotropins, Pituitary; D014316:Tritium