Simplified assay for concanavalin A-dependent bacterial agglutination by using cell surface mutants.
Two cell surface mutants of Escherichia coli isolated through supersensitivity to sodium dodecyl sulfate were found to be agglutinated by concanavalin A in the intact rod form. By using these strains, the assay method for the concanavalin A-dependent agglutination was greatly simplified since neither spheroplast formation nor protease treatment is needed. This rod cell agglutination was characterized and applied for a semiquantitative determination of the amount of bound concanavalin A.
D000371:Agglutination; D000372:Agglutination Tests; D002462:Cell Membrane; D003208:Concanavalin A; D004926:Escherichia coli / Q000276:immunology*; D008722:Methods; D008858:Microscopy, Phase-Contrast; D009154:Mutation
D003208:Concanavalin A