title: Secondary cytotoxic cell response to lymphocytic choriomeningitis virus II. Nature and specificity of effector cells.
abstract: The method described in the previous paper was used to induce secondary responses in spleen cells from CBA/H mice, pre-primed with lymphocytic choriomeningitis (LCM) virus by culturing them with LCM-infected peritoneal cells. The cytolytic effector cells thus generated have been characterized. Effector cells were sensitive to treatment with anti-theta ascitic fluid and complement. Separation procedures based on rosetting of certain categories of lymphocytes with sheep red cells through an Isopaque-Ficoll gradient indicated that effector cells lacked surface immunoglobulin and generally did not bear Fc receptors. Cytolytic activity was restricted by the H-2 gene complex. Killing had single-hit characteristics. All these results suggested that the cells from memory cultures mediating cytolysis were T cells. There was evidence for two T cell subsets, a major subpopulation directed against antigens on infected targets and a minor one directed against antigens on uninfected, H-2-compatible targets. Specificity was present at the infected cell:memory responder and killer:target levels between LCM virus (an arenavirus) and ectromelia virus (a poxvirus).
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001202:Ascitic Fluid / Q000166:cytology; D003601:Cytotoxicity Tests, Immunologic; D007104:Immune Adherence Reaction; D007141:Immunoglobulin Fc Fragments; D007156:Immunologic Memory; D066298:In Vitro Techniques; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D007141:Immunoglobulin Fc Fragments