title: Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro.
abstract: Purified E. coli dnaB and dnaC(D) gene products interact physically and functionally in vitro. This interaction was demonstrated as follows: (a) A complex of dnaB and dnaC(D) gene products was isolated by gel filtration; ATP specifically was required for isolation of the complex. (b) The DNA-independent ribonucleoside triphosphatase activity associated with dnaB gene product was inhibited by dnaC(D) gene product. (c) The dnaC(D) gene product was protected from inactivation by N-ethyl-maleimide by the combination of dnaB gene product and ATP; this protection required ATP specifically.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D005796:Genes
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000255:Adenosine Triphosphate; D005033:Ethylmaleimide