Preparation and neutralization characteristics of an anti-CSF antibody.
Nine of ten rabbits immunized with a partially purified L-cell CSF had demonstrable titers of anti-CSF activity. In vitro the antibody was markedly inhibitory to both post-endotoxin mouse sera and several mouse tissue extracts. CSF containing conditioned media prepared from a number of sources showed variable inhibition suggesting that murine CSF's may be characterized by marked antigenic differences. Human sources of CSF were also inhibited thus indicating a degree of cross-reactivity between murine and human factors. These studies may provide the initial steps toward definition of the role of CSF in vivo.
D000818:Animals; D000906:Antibodies; D002999:Clone Cells; D003115:Colony-Stimulating Factors / Q000276:immunology*; D003470:Culture Media; D003601:Cytotoxicity Tests, Immunologic; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D051379:Mice; D011817:Rabbits; D014020:Tissue Extracts
D000906:Antibodies; D003115:Colony-Stimulating Factors; D003470:Culture Media; D006023:Glycoproteins; D014020:Tissue Extracts