title: Single-strand scissions of chromosomal DNA during commitment to recombination at meiosis.
abstract: Diploid cells of the yeast Saccharomyces cerevisiae induced to undergo meiosis accumulate single-strand scissions in both template and newly synthesized DNA during commitment to genetic recombination. No evidence for accumulation of double-strand breaks during meiosis was obtained. When commitment to recombination is at the full meiotic level there are approximately 70 to 200 single-strand scissions per meiotic cell in which approximately 150 recombination events have been reported to occur.
mesh_terms: D002499:Centrifugation, Density Gradient; D002875:Chromosomes / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004171:Diploidy; D008540:Meiosis; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013172:Spores, Fungal / Q000378:metabolism; D013698:Templates, Genetic; D013997:Time Factors; D014498:Uracil / Q000378:metabolism
chemical_list: D004277:DNA, Single-Stranded; D014498:Uracil