Determination of serum nucleotidase with cytidine monophosphate as substrate, (I).
This paper deals with a new method for the determination of serum nucleotidase (EC 3.1.3.5). The assay is performed with cytidine-5'-monophosphate as substrate, followed by deamination of the generated cytidine. The principle of the method and the determination of the liberated ammonia by the Berthelot indophenol-reaction are comparable to the Persijn--van der Slik method in which adenosine-5'-monophosphate is used as substrate. The correlation between the results obtained with these two methods was found to be good; the new method has the advantage of higher sensitivity.
D000243:Adenosine Deaminase; D000249:Adenosine Monophosphate; D000641:Ammonia; D003564:Cytidine Deaminase; D003597:Cytosine Nucleotides; D004926:Escherichia coli; D007215:Indophenol; D008274:Magnesium; D009708:Nucleotidases / Q000097:blood*; D013053:Spectrophotometry / Q000379:methods
D003597:Cytosine Nucleotides; D000249:Adenosine Monophosphate; D007215:Indophenol; D000641:Ammonia; D009708:Nucleotidases; D000243:Adenosine Deaminase; D003564:Cytidine Deaminase; D008274:Magnesium