New immunofluorescence method for the identification of group a, b, c, e, and g streptococci.
An immunofluorescence method for the identification of groups A, B, C, E, F, and G beta-haemolytic streptococci has been developed, using FITC-labelled F(ab')2 fragments of IgG with optimal staining characteristics. The method is useful for the rapid confirmation of beta-haemolytic streptococci in cultures; in a blind comparison with Lancefield grouping, there was a 97 per cent agreement.
D000818:Animals; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011817:Rabbits; D013045:Species Specificity; D013291:Streptococcus / Q000302:isolation & purification*
D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G