The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens.
Helper cell induction to nonparticle antigens in vitro requires the cooperation of T cells and macrophages, but does not occur if the macrophages are allogenic.  The reasons for this were investigated.  Malfunction of allogenic macrophages was excluded by cultures with their syngenic T cells; suppressor cell induction was excluded by admixture experiments.  Thus, T cells and macrophages only cooperated if they were genetically similar.  The genetic locus (loci) involved was mapped.  Using congenic lines differing only at the H-2 complex, the genetic control of T-macrophage interaction was localized in the H-2 region.  Mice with intra H-2 recombinants were used to map the T-macrophage interaction locus in the I-A region of the H-2 complex (formerly known as poly-D, L-ala-poly-L-lys.  Recombinants were also used to exclude the presence of another T-macrophage locus either the K, I-B, or I-C, SS-Slp, or D regions of the H-2 complex.  Genetic restrictions for T-macrophage interaction in helper cell induction was shown in mice of the H-2-k, d, b, q, s genotypes as well as in H-2 recombinants.  The possible mechanisms and significance of this genetic restriction are discussed.
D000596:Amino Acids; D000818:Animals; D000921:Antibody-Producing Cells; D000941:Antigens; D002352:Carrier Proteins; D002478:Cells, Cultured; D002874:Chromosome Mapping; D006433:Hemocyanins / Q000276:immunology; D006649:Histocompatibility Antigens; D008198:Lymph Nodes / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D011108:Polymers; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000596:Amino Acids; D000941:Antigens; D002352:Carrier Proteins; D006649:Histocompatibility Antigens; D011108:Polymers; D006433:Hemocyanins