title: Mode of replication of plasmid lambda-dv-1.
abstract: About 20 copies of plasmid lambda-dv are perpetuated per host chromosome in Escherichia coli K12 cells.  The mode of DNA replication of this plasmid lambda-dv was studied, using the density-labelling technique followed by banding in DsCl.  It was shown that all the copies of plasmid lambda-dv are potentially capable of replicating roughly once a cell generation.  Their replication occurs by random, that is a copy of the plasmid is taken out at random for replication from a pool, to which the two replicas resulting from replication are returned.  Chloramphenicol did not inhibit the initiation of a new round of replication of the plasmid molecules, indicating that selection from the replication pool does not require concomitant protein synthesis.
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D003090:Coliphages / Q000187:drug effects / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D004267:DNA Viruses / Q000187:drug effects / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism*; D005111:Extrachromosomal Inheritance; D010957:Plasmids; D013997:Time Factors; D014779:Virus Replication
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D002701:Chloramphenicol