[Fibrin removal from mouse immune ascitic fluids to arbovirus using beta-propiolactone].
A modification of acid precipitation of fibrin in immune ascitic fluids (IAF) employing beta-propiolactone has been developed. The procedure removes fibrin completely without affecting the titres of specific antibody. This modification is not inferior to previously described methods for fibrin removal from IAF and has a number of advantages. In addition to removing fibrin from IAF, beta-propiolactone prevents possible virus contamination of IAF and sterilizes the preparation additionally.
D000914:Antibodies, Viral / Q000032:analysis; D001103:Arboviruses / Q000276:immunology*; D001202:Ascitic Fluid / Q000032:analysis / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology; D004660:Encephalitis; D005337:Fibrin / Q000302:isolation & purification*; D007158:Immunologic Techniques; D007202:Indicators and Reagents; D007783:Lactones; D009975:Orthomyxoviridae / Q000276:immunology; D014902:West Nile virus / Q000276:immunology
D000914:Antibodies, Viral; D007202:Indicators and Reagents; D007783:Lactones; D005337:Fibrin
Udalenie fibrina iz immunnykh astsiticheskikh zhidkosteĭ mysheĭ k arbovirusam s pomoshch'iu beta-propiolaktona