Detection of antigens specific for B-lymphoid cultured cell lines with human alloantisera.
Human sera were tested for cytotoxicity to pairs of long-term tissue-cultured cell lines. Each pair had been derived from the same individual and one of the pairs possessed the characteristics of either "T" or "B" cells. The alloantisera used were HL-A-typing reagents or sera obtained from Amish multiparas. Selected cytotoxicity was found against the  B-cell lines by direct testing. Cytotoxicity was abolished by absorption with B-cell line but not by absorption with the T-cell lines. The results suggest that a group of allotypic antigens may be expressed exculsively on human B cells.
D001402:B-Lymphocytes / Q000276:immunology*; D002452:Cell Count; D002460:Cell Line; D002860:Chromium Radioisotopes; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D005826:Genetics, Medical; D006680:HLA Antigens / Q000145:classification*; D006649:Histocompatibility Antigens / Q000145:classification*; D006801:Humans; D007178:Inbreeding; D007518:Isoantibodies; D007519:Isoantigens; D013601:T-Lymphocytes / Q000276:immunology
D002860:Chromium Radioisotopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007518:Isoantibodies; D007519:Isoantigens; D003165:Complement System Proteins