Induction of T cell response to haptens coupled to mycobacteria.
A new method for the induction of cellular immune response to commonly used haptens in the absence of detectable antibody response is described. Different haptens were convalently coupled to Mycobacteria and they were injected into guinea pigs in incomplete Freund's adjuvant. Humoral and cellular immune response to haptens were examined at weekly intervals for 5 weeks. Our results show that a significant anti-hapten cellular response was induced and subsequently elicited by both in vivo (skin test) and in vitro (Lymphocyte transformation and macrophage migration inhibition) assays.
D062365:Aminobenzoates / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D002464:Cell Migration Inhibition; D005260:Female; D006168:Guinea Pigs; D006241:Haptens / Q000008:administration & dosage*; D007111:Immunity, Cellular; D008297:Male; D009161:Mycobacterium / Q000276:immunology*; D009578:Nitrobenzenes / Q000276:immunology; D011233:Precipitin Tests; D012882:Skin Tests; D013601:T-Lymphocytes / Q000276:immunology*
D062365:Aminobenzoates; D006241:Haptens; D009578:Nitrobenzenes