Tissue vitamin B-12 assay by a radioisotope dilution technique.
A method is described for the measurement of vitamin B-12 (B-12) in solid tissues by radioisotope dilution (RID) assay. The method is a modivication using double extraction of a serum B-12 RID assay which uses chicken serum as the B-12 binder. The method was developed and tested using human and bat liver specimens. Double extraction was shown to be more efficient than single extraction, and resulted in complete release of endogenous liver [57Co] B-12 administered to bats. Results using the RID assay in 16 humans and 17 bat liver specimens were compared with those obtained using the Lactobacillus leichmannii microbiological assay. Correlation was good, but the RID assay gave higher results using the microbiological assay appear to be due to inadequate extraction of B-12 from tissues.
D000818:Animals; D001681:Biological Assay; D002685:Chiroptera; D003037:Cobalt Radioisotopes; D005069:Evaluation Studies as Topic; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects; D008099:Liver / Q000032:analysis*; D010206:Papain; D011865:Radioisotope Dilution Technique; D013045:Species Specificity; D014805:Vitamin B 12 / Q000032:analysis* / Q000097:blood / Q000494:pharmacology
D003037:Cobalt Radioisotopes; D010206:Papain; D014805:Vitamin B 12