Quantitative determination of indolic compounds in the rat brain using p-dimethylaminocinnamaldehyde as reagent.
A method is proposed for determining the metabolites of radioactive 5-hydroxytryptophan and of endogenous tryptophan in the rat brain. The compounds are separated by thin-layer chromatography and made visible with p-dimethylaminocinnamaldehyde (DACA) reagent. The conditions studied are those which allow the use of DACA reagent for the quantitative determination of tryptophan metabolites in brain tissue. Amount of 5 ng of serotonin and 20 ng of tryptophan can be determined by densitometry.
D006916:5-Hydroxytryptophan / Q000378:metabolism; D000447:Aldehydes; D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D001933:Brain Stem / Q000032:analysis; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer / Q000379:methods; D002934:Cinnamates; D003720:Densitometry; D004123:Dimethylamines; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007211:Indoles / Q000032:analysis*; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D013057:Spectrum Analysis; D013997:Time Factors; D014364:Tryptophan / Q000378:metabolism
D000447:Aldehydes; D002250:Carbon Radioisotopes; D002934:Cinnamates; D004123:Dimethylamines; D007211:Indoles; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D014364:Tryptophan; C018523:4-dimethylaminocinnamaldehyde; D006916:5-Hydroxytryptophan