Extractable nuclear antigen effect on the DNA anti-DNA reaction and NZB/NZW mouse nephritis.
Extractable nuclear antigen (ENA) is composed of at least two components, one a ribonucleo-protein sensitive to ribonuclease or heat and the other a protein. Antibodies to ENA are associated with a relatively benign clinical course in patients with systemic lupus erythematosus (SLE) in which DNA anti DNA complexes are thought pathogenic. The effect of ENA and anti-ENA on DNA anti-DNA reactions in vitro was studied. ENA effectively inhibited an anti-DNA hemagglutination reaction but no effect was found on binding of radioactive DNA or on the anti-hemocyanin hemagglutination reaction. The inhibitory effect was not abolished by yeast ribonuclease (RNase), heating, or DNase. Anti-ENA HAD NO EFFECT ON ANTI-DNA hemagglutination. In vivo, ENA altered the NZB/NZW mouse nephritis thought to be a model for human SLE nephritis. These results suggest the possiblity of a role for ENA in alteration of diseases due to pathogenic DNA anti-DNA complexes.
D000818:Animals; D000974:Antibodies, Antinuclear; D000941:Antigens; D002467:Cell Nucleus / Q000276:immunology*; D002951:Citrates; D004247:DNA; D003851:Deoxyribonucleases; D004195:Disease Models, Animal; D005260:Female; D006384:Hemagglutination; D006824:Hybridization, Genetic; D008180:Lupus Erythematosus, Systemic / Q000628:therapy*; D051379:Mice; D008815:Mice, Inbred Strains; D009393:Nephritis / Q000276:immunology; D009698:Nucleoproteins; D012260:Ribonucleases
D000974:Antibodies, Antinuclear; D000941:Antigens; D002951:Citrates; D009698:Nucleoproteins; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases