Stimulation of a T-independent primary anti-hapten response in vitro by TNP-lipopolysaccharide (TNP-LPS).
The trinitrophenyl hapten (TNP) has been covalently conjugated to bacterial lipopolysaccharides (LPS) to give TNP-LPS. The site of attachment has been suggested to be in the core polysaccharide and lipid A region of the molecule and approximately 2.4 hapten molecules are bound per monomer LPS molecule. The TNP-LPS has been demonstrated to be immunogenic in vitro at very low concentration. This antigen has further been shown to initiate a T-independent TNP-PFC response. The immunogenicity of TNP-LPS is abrogated by mild alkaline hydrolysis, suggesting a requirement for intact lipid A in the initiation of an immune respose at the very low concentrations of antigen used.
D000468:Alkalies; D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D001665:Binding Sites; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D006736:Horses / Q000276:immunology; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D009596:Nitrophenols / Q000276:immunology*; D011135:Polysaccharides, Bacterial; D011485:Protein Binding; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology; D014529:Uridine / Q000378:metabolism
D000468:Alkalies; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; D009596:Nitrophenols; D011135:Polysaccharides, Bacterial; D014529:Uridine