Chloride transport across isolated skin of Rana pipiens.
The influx of Cl- across isolated frog skin bathed on the outside by 0.8 mM NaCl is about 100 nmol cm-2 h-1, which is approximately twice the Cl- influx in intact animals. The influx consists of diffusion (1%), exchange diffusion (38%), and active transport (60%). About 80% of the influx is independent of Na+ in the outer bath and is also independent of concomitant inward movement of cations. Chloride is exchanged for anions, probably HCO-3. The Cl- transport system is saturable; Vmax is about 200 nmol cm-2 h-1, and Ks is about 0.7 mM Cl-. High external concentrations of NaCl increase unidirectional fluxes of Cl- and urea, indicating a change in paracellular pathways. Active transport of Cl- is temperature sensitive (Q10 equals 2.68) and is inhibited by cyanide, dinitrophenol, iodoacetic acid, iodide, thiocyanate, and acetazolamide. The Na-independent component of JClin was unaffected by amiloride, ouabain, or eserine, all of which inhibit Na+ transport.
D000086:Acetazolamide / Q000494:pharmacology; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001001:Anura; D001693:Biological Transport, Active; D002712:Chlorides / Q000378:metabolism*; D003486:Cyanides / Q000494:pharmacology; D004058:Diffusion; D004140:Dinitrophenols / Q000494:pharmacology; D066298:In Vitro Techniques; D007461:Iodoacetates / Q000494:pharmacology; D007474:Ion Exchange; D008564:Membrane Potentials / Q000187:drug effects; D010042:Ouabain / Q000494:pharmacology; D010424:Pentobarbital / Q000494:pharmacology; D010830:Physostigmine / Q000494:pharmacology; D011894:Rana pipiens / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D013696:Temperature; D014508:Urea / Q000378:metabolism
D002712:Chlorides; D003486:Cyanides; D004140:Dinitrophenols; D007461:Iodoacetates; D010042:Ouabain; D000584:Amiloride; D014508:Urea; D010830:Physostigmine; D010424:Pentobarbital; D000086:Acetazolamide