A new method of obtaining zygotes in Saccharomyces cerevisiae.
Experiments leading to a simple method of mass zygote formation in Saccharomyces cerevisiae are described. This method ordinarily requires only about 3 h of incubation, and consistently yielded 55-65% zygotes in seven different crosses among six different strains. Matings involving one strain required about 4 h of incubation but otherwise the results were comparable.
D000332:Aerobiosis; D003227:Conjugation, Genetic / Q000187:drug effects; D003433:Crosses, Genetic; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013045:Species Specificity; D013997:Time Factors
D003470:Culture Media; D005947:Glucose