Variation of ribosomal proteins with bacterial growth rate.
The composition of ribosomal proteins has been examined as a function of the growth rate of Escherichia coli cells. Seven sets of cultural conditions, utilizing different combinations of carbon and nitrogen sources, were employed to provide a 36-fold spread in growth rate. The cellular content of most of the ribosomal proteins in ribosomes decreased to a similar extent in the very slow-growing cultures. Major exceptions were proteins S6 and L12, which exhibited a much more pronounced decrease , and S21, which exhibited an increase. None of the proteins remained invariant with growth rate.
D000409:Alanine / Q000378:metabolism; D000643:Ammonium Chloride / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002250:Carbon Radioisotopes; D002458:Cell Fractionation; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism; D014316:Tritium; D014461:Ultracentrifugation
D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D003470:Culture Media; D013386:Succinates; D000643:Ammonium Chloride; D014316:Tritium; D001224:Aspartic Acid; D006639:Histidine; D007930:Leucine; D005947:Glucose; D000409:Alanine; D005990:Glycerol