On the role of the H-2 histocompatibility complex in determining the specificity of cytotoxic effector cells sensitized against syngeneic trinitrophenyl-modified targets.
Spleen cells cultured with syngeneic trinitrophenyl (TNP)-modified stimulator cells display a cytotoxic effect against syngeneic TNP-modified targets, but not against modified targets from unrelated H-2 haplotypes.  Targets that share the K and I region of the H-2 complex with the stimulator (or effector) cell are lysed to the same extent as the specific targets, while targets that share the I region only are not.  When only the D region is shared, a weak cytotoxic effect is observed.  Therefore, the stimulator (or effector) and target cell must share the K or D but not the I region of the H-2 complex in order for optimal cytotoxicity to occur.  Spleen cells sensitized to irradiated TNP-modified H-2-allogeneic cells are cytotoxic to these specific cells.  Coculture of F1 hybrid cells with irradiated TNP-modified parental cells result in a cytotoxic effect against only those specific parental cells and not TNP-modified cells from the other parent.  The cytotoxic effect of the F1 effector cells in the cell-mediated lympholysis test is blocked by the addition of unlabeled TNP-modified targets that are H-2 syngeneic with the sensitizing parental strain, but not H-2 syngeneic with the other parental strain.  These data demonstrate that the specificity of the effector cell in this syngeneic cytotoxicity system is directed against altered self H-2-controlled-gene products, rather than a requirement for sharing of histocompatibility genes between effector and target cell in order for lysis to occur.  The role of H-2 antigens in determining the sensitivity of a target cell to T-cell-mediated lysis is discussed.
D000818:Animals; D002588:Cesium Radioisotopes; D002860:Chromium Radioisotopes; D002874:Chromosome Mapping; D003208:Concanavalin A / Q000494:pharmacology; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D005720:Gamma Rays; D006649:Histocompatibility Antigens; D006824:Hybridization, Genetic; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000276:immunology; D009578:Nitrobenzenes / Q000494:pharmacology*; D011830:Radiation Effects; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000528:radiation effects; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*
D002588:Cesium Radioisotopes; D002860:Chromium Radioisotopes; D006649:Histocompatibility Antigens; D009578:Nitrobenzenes; D003208:Concanavalin A; D014302:Trinitrobenzenesulfonic Acid