Biological activity of T4 DNA synthesized in toluene-treated Escherichia coli cells.
T4 DNA synthesized in a toluene-treated cell system can act as the genetic donor in a DNA transformation assay. This material transforms a variety of markers at high efficiency. We present evidence that the genetic activity is due to newly synthesized, double-stranded DNA.
D003090:Coliphages / Q000254:growth & development*; D004261:DNA Replication; D004267:DNA Viruses / Q000254:growth & development; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli / Q000187:drug effects*; D008040:Genetic Linkage; D006378:Helper Viruses; D009154:Mutation; D009691:Nucleic Acid Denaturation; D014050:Toluene / Q000494:pharmacology*; D014170:Transformation, Genetic; D014779:Virus Replication
D004279:DNA, Viral; D014050:Toluene