Carcinogenesis in tissue culture 25: reduced tumorigenicity of alkaline phosphatase-constitutive variants from Chinese hamster ovary cells.
Several subclones with high alkaline phosphatase (ALP) activity were isolated from ALP-negative Chinese hamster ovary cells (CHO-K1). When inoculated into cheek pouch membranes of Syrian golden hamsters treated with anti-hamster thymocyte serum (ATS), ALP-negative CHO-K1 cells produced progressively growing tumors, whereas the cells of ALP-positive subclones did not, although small nodules were formed temporarily. The animals injected CHO-K1 cells died of tumor by 35 days after grafting, and metastases in liver and lung were revealed on autopsy. The histological features of the resultant neoplasms were consistent with fibrosarcoma. In animals transplanted with the cells of ALP-positive subclones, neither tumor death, nor metastasis formation was observed.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000961:Antilymphocyte Serum; D002610:Cheek; D002999:Clone Cells; D006224:Cricetinae; D046508:Culture Techniques; D005260:Female; D005354:Fibrosarcoma / Q000201:enzymology / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology; D008175:Lung Neoplasms / Q000473:pathology; D008297:Male; D009365:Neoplasm Regression, Spontaneous; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology*; D010053:Ovary / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology; D014183:Transplantation, Heterologous
D000961:Antilymphocyte Serum; D000469:Alkaline Phosphatase