Temperature-sensitive RNA polymerase mutants with altered subunit synthesis and degradation.
A temperature-sensitive mutant having a lethal mutation in the gene for the beta subunit of RNA polymerase (nucleosidetriphosphate:RNA  nucleotidyltransferase, EC 2.7.7.6) exhibits an apparent 2- to 3-fold decrease in the rates of both beta and beta' subunit synthesis at the non-permissive temperature, relative to total protein. In contrast, a temperature-sensitive mutant with a lethal mutation in the gene encoding beta' has a 5- to 6-fold increase in the rates of beta and beta' synthesis at 42 degrees. These beta and beta' mutants also exhibit rapid degradation of both subunits at the high temperature.
D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005827:Genetics, Microbial; D007553:Isotope Labeling; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D046911:Macromolecular Substances; D009154:Mutation; D012967:Sodium Dodecyl Sulfate; D013696:Temperature; D013997:Time Factors; D014316:Tritium
D046911:Macromolecular Substances; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D012321:DNA-Directed RNA Polymerases; D007930:Leucine