Preferential production of IgG2 anti-hapten antibody by immunization with hapten-conjugated Escherichia coli.
It is well-known that immunization of guinea pigs with hapten-protein conjugates induces concomitant production of IgG1 and IgG2 anti-hapten antibodies.  However, the synthesis of antibody to 2, 4-dinitrophenyl groups (DNP) was found to be restricted to one of the IgG antibodies (IgG2 antibody) when guinea pigs were immunized through repeated intraperitoneal injections of 0.1 mg of 2, 4-dinitrophenylated Escherichia coli (DNP-E coli).  This selective induction of IgG2 anti-DNP antibody formation occurred both in the presence and absence of Freund's adjuvants, whereas a trace of IgG1 anti-DNP antibody was produced concomitantly on increasing the immunizing dose of DNP-E. coli (3.0 mg) in Freund's complete adjuvant (FAC).
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000942:Antigens, Bacterial; D004140:Dinitrophenols / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology*; D005620:Freund's Adjuvant; D006168:Guinea Pigs; D006241:Haptens; D006386:Hemagglutination Tests; D007114:Immunization; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000096:biosynthesis*; D011863:Radioimmunoassay
D000906:Antibodies; D000942:Antigens, Bacterial; D004140:Dinitrophenols; D006241:Haptens; D007074:Immunoglobulin G; D005620:Freund's Adjuvant