Identification of complement-receptor lymphocytes (B cells) in lymph nodes and tumor infiltrates.
To monitor the distribution of lymphocyte sub-populations infiltrating tumors, a simplified modification of standard techniques was developed to identify the complement-receptor B lymphocytes in frozen tissue sections. This technique is based on binding of antibody-coated bovine erythrocytes by B lymphocytes in the presence of sub-hemolytic concentrations of mouse complement. B lymphocytes can be identified in frozen tissue sections by adherent sensitized bovine erythrocytes. The specificity of the technique for B lymphocytes was established by 1) absence of spontaneous rosette formation with bovine erythrocytes by human T lymphocytes, 2) specific binding of bovine erythrocyte-antibody-complement complexes to B cell regions of normal lymph nodes and spleen and 3) binding of erythrocyte-antibody-complement to a high proportion of chronic lymphocytic leukemia (a B cell leukemia) lymphocytes. Five transitional carcinomas studied had regions of mononuclear infiltration that were virtually devoid of complement-receptor lymphocytes. The results suggest that lymphocytes infiltrating bladder carcinomas may be predominantly T lymphocytes.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001665:Binding Sites; D001666:Binding Sites, Antibody; D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D002417:Cattle; D003176:Complement C3; D003165:Complement System Proteins; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007104:Immune Adherence Reaction; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology*; D011379:Prognosis; D013154:Spleen / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D003176:Complement C3; D003165:Complement System Proteins