Stereoselective reduction of cobalt(III) complexes by bacteria.
A strain of Enterobacter cloacae, isolated from soil, reduces racemic abdtriglycinatocobalt(III), triglycinatocobalt(III), stereoselectively under anaerobic conditions; Washed suspensions oc cells, grown anaerobically in the absence of triglycinatocobalt(III) initially reduce the L-enantiomer stereospecifically under argon yielding a labile Co(II) chelate. An approximately 5-fold increase in the rate of reduction of the complex occurs using cells disrupted by 2-phenylethanol; decomposition now proceeds with a slight preference for the D-enantiomermreduction of triglycinatocobalt(III) under argon by cell-free extracts, prepared by sonication, exhibits similar stereoselectivities to cells disrupted by 2-phenylethanolmhowever, extracts contain a factor capable of reducing D-triglycinatocobalt(III) with high stereoselectivity under molecular hydrogenmthe behaviour of Enterobacter cloacae is compared with that of Aerobacter aerogenes, Proteus vulgaris, Escherichia anaerogenes and Serratia marcescensmstereoselective effects are also observed in the bacterial reduction of other cobalt(III) complexes.
D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism*; D002942:Circular Dichroism; D003035:Cobalt / Q000378:metabolism*; D004754:Enterobacter / Q000378:metabolism; D004755:Enterobacteriaceae / Q000378:metabolism; D004925:Escherichia / Q000378:metabolism; D005998:Glycine; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000378:metabolism*; D010084:Oxidation-Reduction; D011514:Proteus vulgaris / Q000378:metabolism; D012706:Serratia marcescens / Q000378:metabolism; D013045:Species Specificity; D013053:Spectrophotometry; D013237:Stereoisomerism; D013997:Time Factors
D009942:Organometallic Compounds; D003035:Cobalt; D005998:Glycine