[Study of the stability and the degradation of fluorescence in the immunofluorescence technic (FTA-ABS) using pulsed lasers].
The use of two pulsed lasers as excitation sources in the immunofluorescent technique FTA-ABS (fluorescent treponemal antibody absorption) shows that excitation of specimens by continuous or long-duration pulses of light (in the order of a millisecond) degrades the fluorescence in such a way that it is impossible to quantify exactly the fluorescent signal or repeat the analysis. Stability of fluorescence can be achieved by very short excitation pulses (in the order of a microsecond) and this fast fluorescent signal can be detected only by an electronic detection and recording system. With such a system, high precision is obtained by elimination of errors by the human eye.
D005069:Evaluation Studies as Topic; D005453:Fluorescence; D005455:Fluorescent Antibody Technique / Q000295:instrumentation; D007834:Lasers; D013588:Syphilis Serodiagnosis / Q000295:instrumentation / Q000379:methods*
Etude de la stabilité et de la dégradation de la fluorescence dans la technique d'immunofluorescence (FTA-ABS) à l'aide de lasers à impulsions