In vitro synthesis of tRNA precursors and their conversion to mature size tRNA.
Two Escherichia coli tRNA gene clusters, tRNA1Tyr (su3+ and su3-) and tRNA2Tyr, tRNA2Gly (su+36), tRNA3Thr, were transcribed in a purified in vitro system. Evidence indicates that the adjacent tRNA genes are transcribed together as a common precursor of large size, which, on incubation with crude cell extracts, yields mature tRNA molecules.
D001483:Base Sequence; D002474:Cell-Free System; D004926:Escherichia coli / Q000378:metabolism; D008970:Molecular Weight; D009694:Nucleic Acid Precursors / Q000096:biosynthesis*; D009876:Operon; D012343:RNA, Transfer / Q000032:analysis / Q000096:biosynthesis*; D014158:Transcription, Genetic
D009694:Nucleic Acid Precursors; D012343:RNA, Transfer