Immunofluorescence of mitotic spindles by using monospecific antibody against bovine brain tubulin.
Monospecific antibody directed against bovine brain tubulin has been purified by affinity chromatography and tested against soluble tubulin and intact microtubules of brain and mitotic apparatus. Binding of the tubulin antibody to the mitotic spindle of rat kangaroo cells was demonstrated in all stages of mitosis by indirect immunofluorescence.
D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D001923:Brain Chemistry; D002417:Cattle; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D008870:Microtubules / Q000276:immunology*; D008938:Mitosis; D009419:Nerve Tissue Proteins / Q000276:immunology* / Q000302:isolation & purification; D009940:Organoids; D011817:Rabbits / Q000276:immunology; D051381:Rats
D000906:Antibodies; D009419:Nerve Tissue Proteins