A non-destructive method for measuring intestinal cell transit time.
Villous exfoliation of intestinal epithelial cells, which had been previously labeled in the crypt with 125I-iododeoxy-uridine, can be detected by thyroidal accumulation of the liberated 125I-. The latter can be monitored externally. The interval between labeled precursor injection and the steep portion of the thyroid activity accumulation curve corresponds to the intestinal cell transit time, as measured by destructive techniques, in three animal species. The technique allows estimations of intestinal cell transit time to be made on an individual basis, and can be expeditiously applied to large animals. Very small tracer doses are required for detection.
D000367:Age Factors; D000818:Animals; D001345:Autoradiography; D002465:Cell Movement; D003584:Cytological Techniques; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005849:Gerbillinae; D007065:Idoxuridine; D007421:Intestine, Small / Q000166:cytology* / Q000502:physiology; D007457:Iodine Radioisotopes; D051381:Rats; D013961:Thyroid Gland / Q000378:metabolism
D007457:Iodine Radioisotopes; D007065:Idoxuridine