Immune response to liposomal model membranes: restricted IgM and IgG anti-dinitrophenyl antibodies produced in guinea pigs.
Guinea pigs were immunized with liposomal model membranes actively sensitized with 2,4-dinitrophenyl-aminocaproylphosphatidyl-ethanolamine. The immune response was characterized by the formation of both IgM and IgG anti-DNP plaque-forming cells (PFC) which paralleled the appearance of anti-DNP antibodies in the serum. Plaque inhibition by DNP-lysine indicated that the PFC produced after liposomal immunization fell in a much narrower range of avidity groups than the PFC obtained after immunization with DNP-albumin and, in this regard, resembled MOPC 315. The restricted nature of the serum IgG anti-DNP antibodies was confirmed by isoelectric focusing and revealed the expression of a limited number of clones in all cases.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000921:Antibody-Producing Cells; D004140:Dinitrophenols / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006462:Hemolytic Plaque Technique; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007525:Isoelectric Focusing; D008081:Liposomes / Q000276:immunology*; D008239:Lysine / Q000494:pharmacology; D009101:Multiple Myeloma / Q000276:immunology; D009194:Myeloma Proteins / Q000276:immunology; D010047:Ovalbumin; D010954:Plasmacytoma / Q000276:immunology; D012709:Serum Albumin; D012756:Sheep / Q000276:immunology
D004140:Dinitrophenols; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008081:Liposomes; D009194:Myeloma Proteins; D012709:Serum Albumin; D010047:Ovalbumin; D008239:Lysine