Use of erythrocytes sensitized with purified enterotoxin from Vibrio cholerae for the assay of antibody and antibody-forming cells.
A method was developed for the sensitization of ovine erythrocytes with a purified enterotoxin from Vibrio cholerae. Sensitized cells were used for the titration of serum antibody by passive hemagglutination and in a hemolytic plaque assay for both IgM and IgG antibody-secreting cells. Inhibition experiments with various antigens of V. cholerae indicated that the toxin, whether unheated or heat-inactivated, significantly reduced the expected antitoxic plaque-forming cell response, whereas a lipopolysaccharide-rich extract from homologous vibiros was not inhibitory.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000032:analysis*; D000956:Antigens, Viral; D001426:Bacterial Proteins / Q000276:immunology; D003864:Depression, Chemical; D004768:Enterotoxins / Q000008:administration & dosage / Q000302:isolation & purification*; D004912:Erythrocytes / Q000276:immunology*; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006462:Hemolytic Plaque Technique; D006736:Horses / Q000276:immunology; D007106:Immune Sera; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D014734:Vibrio cholerae / Q000276:immunology*
D000906:Antibodies; D000956:Antigens, Viral; D001426:Bacterial Proteins; D004768:Enterotoxins; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M