title: Enhanced uptake of donor DNA by Ca2+ treated Escherichia coli cells.
abstract: The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor exogenous DNA upon Ca2+ treatment. At saturating DNA concentrations the cells were capable to take up irreversibly about 6-10(8) dalton of donor DNA per cell (if to assume that all the cells are competent). About 75% of this DNA was found to be attached to the cytoplasmic membrane. Employing Poisson approximation for distribution of the donor-marker DNA molecules on recipient cells it was found that the efficiency of a marker recombination and expression is about 2-10(-5). The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor 0exogenous
mesh_terms: D002118:Calcium / Q000494:pharmacology*; D002462:Cell Membrane; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000378:metabolism*; D011995:Recombination, Genetic; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D002118:Calcium