The Fc receptor on thymus-derived lymphocytes. I. Detection of a subpopulation of murine T lymphocytes bearing the Fc receptor.
Utilizing the fluorescence-activated cell sorter (FACS) and washed murine antibody-antigen complexes formed in antibody excess, we have demonstrated the presence of the Fc receptor on the surface of a distinct subpopulation of murine T lymphocytes. No differences in intensity of labeling with the complexes was observed when the Fc+ T lymphocytes were compared with Fc+ B lymphocytes. The majority of Fc+ T lymphocytes are small lymphocytes determined by light-scattering characteristics on the FACS. Separating Fc+ from Fc- T lymphocytes from spleens of mice primed 1 wk or 1 mo previously with keyhole limpet hemocyanin (KLH) revealed that the T cells capable of cooperating with DNP-KLH primed B cells to give an adoptive anti-DNP PFC response do not bear the Fc receptor.
D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology*; D002469:Cell Separation; D005260:Female; D005455:Fluorescent Antibody Technique; D007141:Immunoglobulin Fc Fragments; D008198:Lymph Nodes / Q000637:transplantation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000637:transplantation; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000637:transplantation; D014184:Transplantation, Homologous; D010948:Viral Plaque Assay
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D007141:Immunoglobulin Fc Fragments