Differentiating feline T and B lymphocytes by rosette formation.
The number of bone marrow-derived and thymus-dependent lymphocytes in the peripheral blood of felines was assessed. Feline bone marrow-derived lymphocytes were determined by erythrocyte antibody complement (EAC)-rosette formation and membrane immunofluorescence. A good correlation was observed between the cells forming EAC-rosettes (mean of 40.2 +/- 9.7%) and those possessing membrane immunoglobulins (mean of 45.3 +/- 4.1%). Feline thymus-dependent lymphocytes form non-immune (E)-rosettes with rodent erythrocytes (mouse, rat, guinea pig) but not with erythrocytes from humans, monkeys, dogs, mongooses, chickens, rabbits, sheep, and hamsters. An average of 32.3 +/- 992% of the peripheral blood lymphobytes of cats formed rosettes with guinea pig erythrocytes. Antibody blocking, cytotoxicity, and mix-rosette studies demonstrated that guinea pig red blood cells bind specifically to T lymphocytes. Of feline peripheral blood lymphocytes, 41.0 +/- 7.5% showed fluorescent staining with fluorescein-labeled rabbit anti-cat thymus antiserum.
D000818:Animals; D000961:Antilymphocyte Serum; D001402:B-Lymphocytes / Q000276:immunology*; D001667:Binding, Competitive; D002415:Cats / Q000276:immunology*; D002645:Chickens / Q000276:immunology; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D004285:Dogs / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs / Q000276:immunology; D008988:Herpestidae / Q000276:immunology; D007104:Immune Adherence Reaction; D051379:Mice / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D051381:Rats / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D011947:Receptors, Antigen, B-Cell; D003165:Complement System Proteins