Intermediate-term corneal storage for penetrating keratoplasty.
Fifty-seven penetrating keratoplasties were performed using human donor corneal material stored in modified Tissue Culture Medium 199 (TC-199). Fifty-six grafts were clear on the first postoperative day and remained clear for at least three weeks. During the follow-up period (average, 3.7 months), only four additional graft failures occurred. The reduced incidence of early graft edema is attributed to prolonged viability of corneal endothelium maintained in modified TC-199 medium prior to keratoplasty.
D000328:Adult; D003315:Cornea / Q000033:anatomy & histology; D016039:Corneal Transplantation; D003470:Culture Media; D046508:Culture Techniques; D003886:Descemet Membrane / Q000033:anatomy & histology; D004727:Endothelium / Q000033:anatomy & histology; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D013997:Time Factors; D014021:Tissue Preservation; D014184:Transplantation, Homologous
D003470:Culture Media