Rabbit lymphocyte populations responding to haptenic and carrier determinants for DNA synthesis.
Mesenteric lymph node cells from rabbits primed with dinitrophenyl derivative of either Ascaris extract (DNP-Asc) or ragweed fraction D (DNP-Rag) were stimulated by the priming antigen, free homologous carrier, or with the hapten conjugated with rabbit serum albumin (DNP-RSA), and increase in DNA synthesis was observed by 3H-thymidine incorporation. The results showed that both free carrier and DNP-RSA stimulated DNA synthesis. The response of the primed lymph node cells to DNP-homologous conjugate was slightly higher than that to free carrier, but the optimal concentration of both antigens for maximal thymidine incorporation was 10 to 100 mug/ml. This concentration was about 100 times higher than the optimal concentration of the same antigens for maximal antibody response in vitro. The DNA synthetic response to DNP-RSA was much less than that obtained by free carrier, and the optimal concentration of DNP-RSA for the response was comparable to the concentration of DNP-homologous carrier conjugate to induce maximum anti-DNP antibody response. The relative importance of immunoglobulin bearing (B) cells and T cells in the DNA synthetic responses was assessed by fractionating lymph node cells with antigen-coated or anti-immunoglobulin coated  cellular immunosorbent. The results indicated that hapten-specific, immunoglobulin-bearing cells (B cells) are responsible for the DNA-synthetic response to DNP-RSA, whereas B cells play a minimal role in the response to free carrier.
D000042:Absorption; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000888:Antibodies, Anti-Idiotypic; D001200:Ascaris; D001345:Autoradiography; D002352:Carrier Proteins / Q000276:immunology*; D004247:DNA / Q000096:biosynthesis*; D004140:Dinitrophenols / Q000276:immunology; D006041:Goats / Q000276:immunology; D006241:Haptens; D007106:Immune Sera; D007114:Immunization; D007136:Immunoglobulins; D007457:Iodine Radioisotopes; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D011058:Pollen; D011817:Rabbits; D011863:Radioimmunoassay; D012709:Serum Albumin / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology; D014316:Tritium
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D002352:Carrier Proteins; D004140:Dinitrophenols; D006241:Haptens; D007106:Immune Sera; D007136:Immunoglobulins; D007457:Iodine Radioisotopes; D012709:Serum Albumin; D014316:Tritium; D004247:DNA; D013936:Thymidine