The fine structure of the lymphocyte nucleus under conditions of phytohaemagglutinin stimulation.
The nucleus of PHA (phytohaemagglutinin) cultured lymphocytes is studied by EDTA, Thalium-Schiff and uranyl-lead techniques. Scattering of the dense chromatin, increase in the number of NB (nuclear bodies) and nucleolar modifications are established. Transportation is seen of granules similar to perichromatin ones from the nucleus into the cytoplasm. Morphometric investigation reveals a decrease between 7,7 and 26,3% of the dense chromatin and also that the number of PCG (perichromatin granules) to be the greatest in the untransformed PHA lymphocytes, e.g. B lymphocytes. It is established in new born chickens that the lymphocytes of the bourse of Fabricius are richer in PCG than these of the thymus.
D000818:Animals; D001402:B-Lymphocytes / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure*; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D006168:Guinea Pigs; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure*; D013601:T-Lymphocytes / Q000648:ultrastructure
D002843:Chromatin; D037102:Lectins