Detection of virus-specific antibody-forming cells of mice immunized with Newcastle disease virus.
The hemolytic plaque assay was adapted to the detection of antibodies to Newcastle disease virus (NDV) in an in vivo, an in vitro system, and a combined in vivo-in vitro system. Several conditions were tested for coupling of sheep erythrocytes to NDV and for the kinetics of plaque formation in the in vivo and in vitro systems. The one set of conditions which provided the best responses is presented. The effect of multiple injections of NDV into mice on plaque formation was optimized.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D006462:Hemolytic Plaque Technique; D007114:Immunization; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D009522:Newcastle disease virus / Q000276:immunology*; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013634:Tannins
D000914:Antibodies, Viral; D013634:Tannins