Blue dextran: influence on chromatographic profile and immunoreactivity of human follicle-stimulating hormone (HFSH).
When FSH is chromatographed on G-100 Sephadex in the absence of Blue Dextran, it elutes as a single peak in the region of monomeric FSH. The addition of Blue Dextran causes a significant portion of FSH, iodinated or uniodinated, to elute in the region of the void volume. When standard curves for FSH are performed under identical conditions, with tracer 125I-FSH from the void volume, and from the FSH monomer region, maximal immunoreactivity was significantly greater for the Blue Dextran-associated tracer.
D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D002850:Chromatography, Gel; D003911:Dextrans / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000276:immunology* / Q000378:metabolism; D006041:Goats / Q000276:immunology; D006801:Humans; D007457:Iodine Radioisotopes; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D003911:Dextrans; D007457:Iodine Radioisotopes; D005640:Follicle Stimulating Hormone