Synthesis of complex forms of bacteriophage phiX174 double-stranded DNA in a temperature-sensitive dnaC mutant of Escherichia coli C.
Fast-sedimenting forms of bacteriophage phiX174 double-stranded replicative-form DNA observed in normal infections continued to accumulate at the nonpermissive temperature in a temperature-sensitive dnaC mutant of Escherichia coli. These complex molecules accounted for up to half of the DNA synthesized during short pulses at the nonpermissive temperature. They were the dead-end products of DNA synthesis, not intermediates in normal replicative-form replication. The data suggest that these higher-than-normal-molecular-weight DNA molecules result from abnormal initiation of phiX174 replicative-form DNA replication.
D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000378:metabolism*; D004261:DNA Replication; D004267:DNA Viruses; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism*; D008854:Microscopy, Electron; D008970:Molecular Weight; D009154:Mutation; D013696:Temperature
D004269:DNA, Bacterial; D004279:DNA, Viral