Enzymic in vitro repair and chemical nature of DNA chain breaks induced by incorporated phosphorus-32P decay.
In vitro repair of single strand breaks in T4 and phage DNA caused by 32p decay was studied. Zone centrifugation procedure showed that polynucleotide kinase, ligase enzyme system failed to repair 32P-damages. It was found that damaged DNA contained gaps and could be repaired by DNA-polymerase I, polynucleotide ligase treatment.
D002470:Cell Survival; D003090:Coliphages; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004260:DNA Repair; D004267:DNA Viruses; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism; D011118:Polynucleotide Ligases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D010735:Phosphoglycerate Kinase; D004254:DNA Nucleotidyltransferases; D011118:Polynucleotide Ligases