Cellular content of ribonucleic acid and protein in Saccharomyces cerevisiae as a function of exponential growth rate: calculation of the apparent peptide chain elongation rate.
The average cellular content of ribonucleic acid and protein was determined in cultures of Saccharomyces cerevisiae growing exponentially at different rates in a variety of media. Estimations of the proportion of total cellular ribonucleic acid that is made up of ribosomal ribonucleic acid were used to calculate the average number of ribosomes per cell at the different growth rates. The fraction of ribosomes actively engaged in translation was estimated by sucrose gradient centrifugation of ribosomes and polysomes. These data were used in a calculation of the apparent time taken for the addition of an amino acid to the growing polypeptide chain; this value was found to vary linearly with growth rate over a fivefold range of doubling times.
D000225:Adenine / Q000378:metabolism; D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D005656:Fungal Proteins / Q000032:analysis / Q000096:biosynthesis*; D008239:Lysine / Q000378:metabolism; D010441:Peptide Chain Elongation, Translational; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000032:analysis; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism; D013053:Spectrophotometry; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D002250:Carbon Radioisotopes; D005656:Fungal Proteins; D012335:RNA, Ribosomal; D014316:Tritium; D014498:Uracil; D012313:RNA; D000225:Adenine; D008239:Lysine