title: Changes in morphogenesis and developmental enzyme levels in Dictyostelium discoideum after gamma irradiation.
abstract: Changes in the levels of specific activity of two enzymes believed to be involved in developmental regulation were observed after irradiating differentiating cells of Dictyostelium discoideum. Stimulation of the levels of specific activity of alkaline phosphatase occured after irradiation at the beginning of development and at the end of the aggregation period, but not after irradiation at the beginning of aggregation. A stimulation in UDP-glucose pyrophosphorylase specific activity was also observed, but to a lesser extent and only after irradiation at the end of aggregation. Dose-dependent delays in the appearance of peaks of specific activity were noted. The delay per unit dose was less when irradiation took place at the beginning of development as opposed to the beginning or end of the aggregation period. Radiation-induced delays in progression through visible developmental stages were almost identical to delays in enzyme appearance. Other radiation effects on morphogenesis included the induction of a migratory slug phase.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D003037:Cobalt Radioisotopes; D004023:Dictyostelium / Q000201:enzymology / Q000254:growth & development / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D009235:Myxomycetes / Q000528:radiation effects*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D011830:Radiation Effects; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000378:metabolism*
chemical_list: D003037:Cobalt Radioisotopes; D009713:Nucleotidyltransferases; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase; D000469:Alkaline Phosphatase