title: Association of human alpha1-antitrypsin with anhydrotrypsin.
abstract: Unlike in the reaction of anhydrotrypsin and soybean trypsin inhibitor, human alpha1-antitrypsin was found not to form a complex with bovine anhydrotrypsin. This was shown using three different methods: electrophoresis, affinity chromatography on an anhydrotrypsin-coupled Sepharose column and equilibrium competitive binding assay. These results indicate the importance of the active site serine of trypsin in formation of a complex with alpha1-antitrypsin.
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002417:Cattle; D006801:Humans; D011485:Protein Binding; D012694:Serine; D014357:Trypsin / Q000378:metabolism; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000378:metabolism*
chemical_list: D000515:alpha 1-Antitrypsin; C011661:anhydrotrypsin; D012694:Serine; D014360:Trypsin Inhibitor, Kunitz Soybean; D014357:Trypsin