Light-scattering study of the temperature dependence of Escherichia coli motility.
Two light-scattering techniques are used to study the temperature dependence of translational and rotational motility in Escherichia coli. The method of number fluctuation spectroscopy is developed theoretically and experimentally to measure the translational swimming speed of a smooth swimming strain of E. coli. Interference fluctuation techniques are used to study the rotational component of the motion. The results demonstrate that the thrust remains proportional the the torque generated by the flagella throughout the range studied and also show that relative changes in translational swimming speed may be inferred from the dynamics of rotational motion.
D002465:Cell Movement; D004926:Escherichia coli / Q000502:physiology*; D005407:Flagella; D008027:Light; D008433:Mathematics; D012399:Rotation; D012542:Scattering, Radiation; D013696:Temperature