A simple method for screening human T and B lymphocyte alloantibodies.
An sRBC-hemolysin monolayer was employed for separating human lymphocytes into two sub-populations, adherent cells (predominantly B cells) and non-adherent cells (predominantly T cells). The separated cells were then tested simultaneously against predispensed sera by complement-mediated cytolysis for the presence of alloantibodies to either T or B cells. A total of 372 sera were screened. Antibodies specific for B cell antigens were found in 19 sera and 9 sera were found to contain antibodies to T-specific antigens.
D001402:B-Lymphocytes / Q000276:immunology*; D001665:Binding Sites; D002448:Cell Adhesion; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens; D006801:Humans; D007104:Immune Adherence Reaction; D007141:Immunoglobulin Fc Fragments; D007518:Isoantibodies / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*
D006680:HLA Antigens; D007141:Immunoglobulin Fc Fragments; D007518:Isoantibodies