Enhancement of reaginic antibody formation in the mouse by concanavalin A.
SWR mice injected with Concanavalin A (Con A) and Ovalbumin (Ov) one immediately after the other or with mixtures of Con A and Ov produced reaginic antibodies to Ov (titer 1:80) after primary immunization and reaginic antibody levels of 1:640 after secondary immunization. Mixtures of Con A and Ov were shown to contain soluble complexes which could be precipitated by 20% polyethylene glycol (PEG). Mixtures of Con A and human serum albumin or Con A and bovine serum albumin did not form soluble complexes and did not induce detectable reagin formation. The possible role of soluble of Con A-Ov complexes in stimulating reaginic antibody is discussed.
D000276:Adjuvants, Immunologic; D000818:Animals; D000906:Antibodies; D000917:Antibody Formation / Q000187:drug effects*; D000961:Antilymphocyte Serum; D003208:Concanavalin A / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D010047:Ovalbumin; D010323:Passive Cutaneous Anaphylaxis; D011092:Polyethylene Glycols; D051381:Rats; D011935:Reagins; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D013601:T-Lymphocytes / Q000276:immunology
D000276:Adjuvants, Immunologic; D000906:Antibodies; D000961:Antilymphocyte Serum; D011935:Reagins; D012709:Serum Albumin; D003208:Concanavalin A; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D010047:Ovalbumin