Rosétte formation between human b lymphocytes and zymosan-C3 complexes using different complement sources.
Sera from five animal species were studied as complement source in the preparation of zymosan-C3 complexes used to detect human B lymphocytes. There was no significant difference in the determination of the percentage of ZC rosette forming cells due to differences in complement source. Human B lymphocytes could not discriminate C3 from different species studied. Every one of these sources of complement may be used interchangeably without altering the percentage of B cells detected by this method.
D000293:Adolescent; D000328:Adult; D000818:Animals; D001402:B-Lymphocytes; D001772:Blood Cell Count; D003176:Complement C3 / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007104:Immune Adherence Reaction; D008297:Male; D051379:Mice; D008875:Middle Aged; D011817:Rabbits; D012756:Sheep; D015054:Zymosan / Q000378:metabolism*
D003176:Complement C3; D003165:Complement System Proteins; D015054:Zymosan