Localization of contractile-dependent Ca: comparison of Mn and verapamil in cardiac and skeletal muslce.
The effects of two excitation-contraction uncoupling agents, manganese (Mn) and verapamil, are compared in heart and fast-twitch skeletal muscle. Particular attention is given to the effect of the agents on the first contraction following a period of quiescence when the agents are administered during the quiescent period. Mn significantly diminishes dP/dt of the first beat in heart muscle, whereas verapamil has no significant effect. Neither Mn nor verapamil has a significant effect on the first postquiescent contraction in skeletal muscle, though verapamil produces a diminution of dP/dt in subsequent contractions in both tissues. A comparison of the effect of the agents on 45Ca exchange in heart cells in tissue culture indicates that Mn induces a rapid displacement of a rapidly exchangeable component of heart-cell calcium with subsequent inhibiton of influx. Verapamil, by contrast, produces no rapid displacement but only an inhibiton of influx. The functional and Ca-exchange effect of the two agents are compatible with a model which places most of the contractile-dependent Ca at the cellular surface in heart muscle and at deeper, intracellular sites in fast-twitch skeletal muscle.
D000818:Animals; D001001:Anura; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D046508:Culture Techniques; D008345:Manganese / Q000378:metabolism* / Q000494:pharmacology; D008954:Models, Biological; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000378:metabolism*; D009200:Myocardial Contraction / Q000187:drug effects*; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D011817:Rabbits; D011894:Rana pipiens; D014700:Verapamil / Q000378:metabolism* / Q000494:pharmacology
D008345:Manganese; D014700:Verapamil; D002118:Calcium