[Electrophoretic mobility of human lymphocytes purified by nylon columns and spontaneous rosettes].
Cell electrophoresis enables the separation of the lymphocytes in normal human blood into two principal groups, as a function of their migration speed in relation to 1 mum.sec -1..v-1.cm. In 42 healthy adults, 19,9 % of the lymphocytes have a slower migration, and 80,1 % a faster migration than the reference speed. Two known methods are used for the selection of the lymphocytic populations : spontaneous rosetting with sheep red blood cells, a property of the T lymphocytes, and the adherence to nylon wool columns, which is dominant in the case of B lymphocytes. The cells which do not form spontaneous rosettes, and the cells adhering to nylon wool columns have above all a slow migration. On the contrary, cells which do not adhere to nylon columns have a fast migration. These arguments are in favour of the T nature of the rapid migrating lymphocytes, and of the B nature of the slow migrating lymphocytes.
D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes; D002469:Cell Separation / Q000379:methods*; D002846:Chromatography, Affinity; D004586:Electrophoresis; D005260:Female; D006384:Hemagglutination; D006801:Humans; D007104:Immune Adherence Reaction; D008214:Lymphocytes; D008297:Male; D008875:Middle Aged; D009757:Nylons; D013601:T-Lymphocytes
D009757:Nylons
Migration électrophorétique de lymphocytes humains purifiés par colonnes de nylon et rosettes spontanées