Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates.
A new group of mutants has been isolated which, during short pulses, incorporate (3-H)thymidine into DNA fragments that are substantially smaller than Okazaki fragments. These small fragments can be chased into DNA of high-molecular-weight, and thus may be precursors in DNA replication, During longer pulses, label also appears in DNA of higher molecular weight, although at an abnormally slow rate. The mutations map at a previously undescribed locus (dnaS) at 72 min on the E. coli chromosome.
D002499:Centrifugation, Density Gradient; D002874:Chromosome Mapping; D004254:DNA Nucleotidyltransferases / Q000032:analysis; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005092:Exonucleases / Q000032:analysis; D005827:Genetics, Microbial; D007553:Isotope Labeling; D009154:Mutation; D011118:Polynucleotide Ligases / Q000032:analysis; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases; D005092:Exonucleases; D011118:Polynucleotide Ligases; D013936:Thymidine