Temperature-sensitive mutants of murine leukemia virus. V. Impaired leukemogenic activity in vivo.
The in vivo biologic activities of a clonal isolate of the Rauscher strain of murine leukemia virus and several conditional lethal mutants with impaired replicative functions at the non-permissive temperature were investigated. The parental virus induced a rapidly developing lymphoid leukemia in a high percentage of NIH Swiss mice inoculated as newborns. Mice inoculated in parallel with temperature-sensitive mutant viruses showed no evidence of disease. The implications of these findings with regard to the requirements for type-C virus replication to permit oncogenic transformation of lymphoid cells in vivo are discussed.
D000818:Animals; D000921:Antibody-Producing Cells; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006462:Hemolytic Plaque Technique; D007942:Leukemia, Experimental / Q000209:etiology*; D007945:Leukemia, Lymphoid / Q000209:etiology; D051379:Mice; D009154:Mutation; D011925:Rauscher Virus; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D013696:Temperature; D014779:Virus Replication