title: Tryptophanase of fecal flora as a possible factor in the etiology of colon cancer.
abstract: Twenty-three strains of intestinal anaerobes obtained from two laboratories were examined for indole production from tryptophan. Among the 23 isolates tested, three of Bacteroides fragilis thetaiotaomicron and one Citrobacter sp. were indole positive. The tryptophanase of the indole-positive strains of intestinal anaerobes was inducible by tryptophan and was susceptible to glucose repression. The products of tryptophanase activity were formed in stoichiometric amounts by dialyzed, freshly prepared extracts. The tryptophan concentration and tryptophanase activity in feces from rats on an all-meat diet were significantly higher than those in feces from rats on a normal diet. The results indicated that the higher tryptophanase activity in the feces of rats fed an all-meat diet is due to the inducibility of this enzyme by tryptophan and is not due to any inhibitor in the feces of rats on a normal diet. The results also suggested that a population with a diet rich in meat has a greater chance for exposure to possible carcinogens such as indole and other tryptophan metabolites. This agrees with the hypothesis, based on epidemiologic data, that a high intake of meat may be related to the development of colon cancer in man.
mesh_terms: D001439:Bacteroides / Q000201:enzymology / Q000302:isolation & purification; D002954:Citrobacter / Q000201:enzymology* / Q000302:isolation & purification; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000209:etiology / Q000382:microbiology; D004032:Diet; D004790:Enzyme Induction; D004794:Enzyme Repression; D004925:Escherichia / Q000201:enzymology*; D005243:Feces / Q000201:enzymology* / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D008190:Lyases / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism; D014368:Tryptophanase / Q000378:metabolism*
chemical_list: D007211:Indoles; D011773:Pyruvates; D014364:Tryptophan; D008190:Lyases; D014368:Tryptophanase; D005947:Glucose