Effect of chronic ethanol ingestion on mitochondrial protein synthesis in Sinclair(S-1) miniature swine.
The effect of chronic ethanol ingestion on hepatic mitochondrial protein synthesis in miniature swine was investigated. After 3 wk on test, there was no decrease in the rate of amino acid incorporation between livers of control and ethanol consuming pigs. After 6 wk on test, there was a 33% decrease in rate of hepatic mitochondrial membrane protein synthesis in livers of experimental pigs, as compared with controls. When analyzed by SDS-gel electrophoresis, a decrease in labeling of the 40,000 mol wt peak, from both whole membrane protein and proteolipid protein profiles, was observed for livers from ethanol consuming pigs as compared with controls.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004586:Electrophoresis; D000431:Ethanol / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008074:Lipoproteins / Q000096:biosynthesis; D008930:Mitochondria, Liver / Q000378:metabolism*; D014176:Protein Biosynthesis; D013552:Swine; D013997:Time Factors
D008074:Lipoproteins; D000431:Ethanol; D007930:Leucine