Studies on the common active site of growth hormone. Revision of the amino acid sequence of an active fragment of bovine growth hormone.
A fragment, A-II, isolated from a component of a tryptic digest of bovine growth hormone has growth-promoting activity in rats and metabolic activity in humans similar to human growth hormone. The amino acid sequence of this peptide has been reinvestigated and revised. The 38-amino acid peptide was cleaved with cyanogen bromide, chymotrypsin, and trypsin. The amino acid sequences were then established by Edman degradation as well as with overlapping peptides; Homology in the sequence was good between this bovine growth hormone fragment and peptides occurring in ovine growth hormone, human growth hormone, and human chorionic somatomammotropin.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D006063:Chorionic Gonadotropin; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin; D013006:Growth Hormone; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D012756:Sheep; D013045:Species Specificity; D014357:Trypsin
D000596:Amino Acids; D006063:Chorionic Gonadotropin; D010446:Peptide Fragments; D013006:Growth Hormone; D002918:Chymotrypsin; D014357:Trypsin