title: Characterization of Newcastle disease viruses isolated from migratory waterfowl in the Atlantic flyway.
abstract: Four isolants of Newcastle disease virus (NDV) obtained from free-flying Canada geese in the Atlantic flyway were characterized and compared with the B1, LaSota, Roakin, and Texas-GB strains of NDV. The 4 isolants were identified as lentogenic strains on the basis of embryo mean death times of greater than 100 hours and relative lack of pathogenicity for 1-day-old and 3-week-old chickens. The hemagglutinin of 2 of the isolants was stable at 56 C for 15 minutes, 1 for 30 minutes, and the other for at least 2 hours. When held at 4 C for 24 hours, none of the 4 isolants could be eluted from chicken erythrocytes. All 4 of the isolants produced plaques in chicken embryo fibroblast cell cultures without additives, whereas the B1 and LaSota strains did not. The 4 lentogenic NDV isolants from Canada geese differed in many respects from currently used commerical vaccine strains (B1 and LaSota).
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D002988:Cloaca / Q000382:microbiology; D004912:Erythrocytes / Q000276:immunology; D005347:Fibroblasts; D005777:Geese; D006389:Hemagglutinins, Viral; D009521:Newcastle Disease / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology*; D009522:Newcastle disease virus / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors; D014132:Trachea / Q000382:microbiology; D010948:Viral Plaque Assay
chemical_list: D006389:Hemagglutinins, Viral