Studies on equine immunoglobulins. IV. Immunoglobulins of the  donkey.
Donkey IgGa was isolated in purified form from normal and immune donkey sera by column chromatography on DEAE-cellulose. Isolated donkey IgGa and mixtures of (IgGa+IgGb) were used as antigens to prepare rabbit reagents specific for equine IgGa or IgGb. Antibodies present in sera obtained from a single donkey at various times during the course of hyperimmunization with BSA were isolated by immuno-adsorption. The class or subclass of immunoglobulins present among isolated, donkey anti-BSA antibodies was determined by use of specific rabbit anti-equine immunoglobulin reagents. The homologues of horse IgGa, IgGb, IgGc and IgA were identified in normal donkey, mule, hinny and zebra serum.
D000818:Animals; D000941:Antigens; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D006736:Horses / Q000276:immunology; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G / Q000302:isolation & purification; D007136:Immunoglobulins / Q000302:isolation & purification*; D010527:Perissodactyla / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine
D000941:Antigens; D007106:Immune Sera; D007070:Immunoglobulin A; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007136:Immunoglobulins; D012710:Serum Albumin, Bovine