Fluorometric and GLC analyses of aristolochic acid.
Aristolochic acid is a natural product with possible implication in Balkan endemic nephropathy. A convenient fluorometric assay for the compound is described based on reduction to the lactam and measurement of the intensity of fluorescence. The limit of sensitivity was 0.05 mug/ml. A GLC assay is also described, based on flash methylation of aristolochic acid and its lactam using trimethylanilinium hydroxide. Conditions for optimum performance with a sensitivity limit of 1-5 mug/ml are described.
D000814:Aniline Compounds; D034341:Aristolochic Acids; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004148:Dioxolanes / Q000032:analysis / Q000652:urine; D005470:Fluorometry; D007769:Lactams / Q000032:analysis; D008745:Methylation; D009574:Nitro Compounds / Q000032:analysis / Q000652:urine; D010084:Oxidation-Reduction; D010616:Phenanthrenes / Q000032:analysis* / Q000652:urine; D010944:Plants / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D000814:Aniline Compounds; D034341:Aristolochic Acids; D004148:Dioxolanes; D007769:Lactams; D009574:Nitro Compounds; D010616:Phenanthrenes; C000228:aristolochic acid I