Substitution of cellular fatty acids in yeast cells by the antibiotic cerulenin and exogenous fatty acids.
Cell growth of Saccharomyces cerevisiae ATCC 12341 inhibited by the antibiotic cerulenin, a specific inhibitor of fatty acid and sterol syntheses, was reversed by various exogenous fatty acids. Myristic acid (14 : 0), pentadecanoic acid (15 : 0), palmitic acid (16 : 0), and oleic acid (18 : 1) reversed effectively the growth inhibition by cerulenin. When these cells were reversed by adding pentadecanoic acid, over 90% of native even-numbered fatty acids was substituted by odd-numbered fatty acids. Those in the cells reversed by adding oleic acid were almost all unsaturated fatty acids. Cerulenin did not inhibit either elongation or desaturation systems in S. cerevisiae.
D000935:Antifungal Agents / Q000494:pharmacology*; D002569:Cerulenin / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013329:Structure-Activity Relationship
D000935:Antifungal Agents; D005227:Fatty Acids; D002569:Cerulenin