title: Cleavage of adenovirus messenger RNA and of 28S and 18S ribosomal RNA by RNase III.
abstract: Escherichia coli ribonuclease III cleaves adenovirus messenger RNA and mammalian 28S and 18S ribosomal RNA. Fragmentation is not random, but in each case a specific collection of products is generated. This points to the potential use of the enzyme as a tool for specific fragmentation of RNA. Cleavage by RNase III abolishes the capability of adenovirus messenger RNA to direct cell-free synthesis of virus polypeptides.
mesh_terms: D000256:Adenoviridae / Q000032:analysis*; D002460:Cell Line; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012367:RNA, Viral; D012260:Ribonucleases / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012367:RNA, Viral; D012260:Ribonucleases