Studies on the production of MIF and mitogenic factor using highly purified human T and B lymphocytes.
Human lymphocytes were separated into highly purified populations using an immunoadsorbent column technique. It was previously reported that both T and B cells exhibited increased 3H-thymidine incorporation in response to PHA, Con A and pokeweed mitogens, whereas only T cells showed increased incorporation in response to specific antigen. In the present studies, the cellular basis of MIF and mitogenic factor production was studied. Both T and B cells produced MIF in response to antigen. The MIF produced by both T and B cells elutes from Sephadex G-100 columns in the same fraction. Studies using BUdR and light suggest that the T cell which produces MIF is also a proliferating cell, whereas the B cell producing MIF is not. Only T cells produce mitogenic factor in response to antigen. The mitogenic factor produced, however, causes both T and B cell populations to increase 3H-thymidine incorporation. The present studies indicate that antigen induced mitogenic factor production and increased 3H-thymidine incorporation are properties of T cells per se, whereas antigen-induced MIF is made by both T and B cells.
D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D001402:B-Lymphocytes / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002175:Candida; D006801:Humans; D008027:Light; D008222:Lymphokines / Q000096:biosynthesis*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis*; D008934:Mitogens / Q000096:biosynthesis*; D013299:Streptodornase and Streptokinase; D013601:T-Lymphocytes / Q000378:metabolism*; D014373:Tuberculin
D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D008222:Lymphokines; D008263:Macrophage Migration-Inhibitory Factors; D008934:Mitogens; D014373:Tuberculin; D013299:Streptodornase and Streptokinase; D001973:Bromodeoxyuridine