Synthesis time of beta-galactosidase in Escherichia coli B/r as a function of growth rate.
By analysing the kinetics of beta-galactosidase accumulation after induction, the synthesis time of beta-galactosidase in Escherichia coli B/r was found to be 75s in rapidly growing cells (1.36 and 2.1 doublings/h), and 90s in slowly growing cells (0.63 doubling/h). These values correspond to peptide-chain-elongation rates of 16 and 13 amino acids/s respectively, in agreement with previous findings, indicating that the peptide-chain growth rate is constant (presumably maximal) in fast-growing bacteria, but decreased in slowly growing bacteria [Forchhammer & Lindahl (1971) J. Mol. Biol. 55, 563-568].
D000596:Amino Acids / Q000378:metabolism; D003470:Culture Media; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D005696:Galactosidases / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D010441:Peptide Chain Elongation, Translational; D013386:Succinates / Q000378:metabolism
D000596:Amino Acids; D003470:Culture Media; D013386:Succinates; D005696:Galactosidases; D005947:Glucose