Autoantigens in human sweat: purification and characterization of the D-group antigens.
We have found several heteropolysaccharides in human thermal sweat which are both autoantigenic and isoantigenic for a majority of healthy adults. They are present in trace amounts and are difficult to isolate. We have facilitated extraction through the use of a DEAE-Sephadex batch technique. One of the major antigenic fractions, the D group, has been purified and characterized. It contains 88.5 per cent carbohydrate as uronic acid, hexosamine, methyl pentose, and glucose. The protein content was 12.5 per cent.
D000941:Antigens / Q000032:analysis* / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000032:analysis; D001324:Autoantigens / Q000302:isolation & purification*; D001419:Bacteria / Q000302:isolation & purification; D002241:Carbohydrates / Q000032:analysis; D002712:Chlorides / Q000032:analysis; D003429:Cross Reactions; D004926:Escherichia coli / Q000276:immunology; D005947:Glucose / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000032:analysis; D008722:Methods; D010429:Pentoses / Q000032:analysis; D011506:Proteins / Q000032:analysis; D013542:Sweat / Q000032:analysis / Q000276:immunology* / Q000382:microbiology; D014574:Uronic Acids / Q000032:analysis
D000941:Antigens; D000942:Antigens, Bacterial; D001324:Autoantigens; D002241:Carbohydrates; D002712:Chlorides; D006595:Hexosamines; D008055:Lipids; D010429:Pentoses; D011506:Proteins; D014574:Uronic Acids; D005947:Glucose