Comparison of the chemical composition of glomerular and tubular basement membranes obtained from human kidneys of diabetics and non-diabetics.
Glomerular basement membrane (GBM) and tubular basement membrane (TBM) were prepared from human kidneys of diabetics and non-diabetics, and their chemical composition was compared. GBM from diabetics contained a larger amount of hydroxyproline than that from non-diabetics, and smaller amounts of half-cystine, glucose, mannose and sialic acid. On the other hand, TBM from diabetics contained larger amounts of hydroxylysine, methionine, galactose, hexosamine and phospholipid phosphorus than non-diabetics, and smaller amounts of half-cystine, valine, leucine, lysine and histidine. No significant difference was observed in the contents of other components examined in this study between the corresponding membrane obtained from diabetics and non-diabetics. The observed changes may be due to alteration of the tissues in diabetes mellitus.
D000328:Adult; D000368:Aged; D000596:Amino Acids / Q000032:analysis; D001485:Basement Membrane / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D003553:Cystine / Q000032:analysis; D003920:Diabetes Mellitus / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000378:metabolism; D005260:Female; D006801:Humans; D006900:Hydroxylation; D006901:Hydroxylysine / Q000032:analysis; D007678:Kidney Glomerulus / Q000032:analysis*; D007684:Kidney Tubules / Q000032:analysis*; D008239:Lysine / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000032:analysis
D000596:Amino Acids; D002241:Carbohydrates; D010743:Phospholipids; D006901:Hydroxylysine; D003553:Cystine; D008239:Lysine