Isolation and characterization of mutants with a feedback resistant N-acetylglutamate synthase in Escherichia coli K 12.
Mutants with a feedback resistant N-acetylglutamate synthase have been isolated from a proA/B, argD, argR strain by screening for proline excretion on minimal medium with arginine. The feedback resistant character of three mutants was transduced into an argA (N-acetylglutamate synthase negative) strain. It was cotransducible with argA at a frequency of greater than 99%. N-acetylglutamate synthase extracted from the three mutants was approximately one hundred times less sensitive to L-arginine than the enzyme from the feedback sensitive parent strain.
D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology*; D005838:Genotype; D005970:Glutamate Synthase / Q000032:analysis*; D009154:Mutation; D011995:Recombination, Genetic; D000637:Transaminases / Q000032:analysis*; D014161:Transduction, Genetic
D005970:Glutamate Synthase; D000637:Transaminases