title: Identity of rat kidney and urine alkaline phosphatase actions and relation to the effect of nitrofuranfuradroxyl.
abstract: The activities of alkaline phosphatases of kidney and urine appear similar and identical in action. Both are magnesium-dependent and inhibited by higher concentrations of 2-glycerophosphate. A band of similar mobility is obtained on electrophoresis of kidney and urine enzymes. In vitro magnesium competitively inhibits their activities when the ratio of Mg-+2 ions/2-glycerophosphate is above a definite level. Their activities are not affected by exogenous zinc and follow the Michaelis-Menten equation only when attention is given to the ratio of Mg-+2 ions/substrate. Nitrofuranfuradroxyl is a mixed type inhibitor showing a second-order rate of reaction with kidney and urine phosphatases.
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood / Q000378:metabolism* / Q000652:urine; D000818:Animals; D005994:Glycerophosphates / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D009581:Nitrofurans / Q000494:pharmacology*; D009928:Organ Specificity; D051381:Rats; D012664:Semicarbazones / Q000494:pharmacology
chemical_list: D005994:Glycerophosphates; D009581:Nitrofurans; D012664:Semicarbazones; D000469:Alkaline Phosphatase; D008274:Magnesium