Effects of growth conditions on thymidine nucleotide pools in Escherichia coli.
The cellular levels of thymidine nucleotide derived from [3H]thymine or [3H]thymidine were followed under various environmental conditions with a thymine-requiring mutant of Escherichia coli. It was shown that the pool sizes varied greatly with the growth conditions; that is, with growth temperature, inhibition of DNA synthesis of replacement of thymine with thymidine. In the strain used here, the level of compound X, presumably dTDP-sugar, was very much higher than those of other thymidine nucleotides. It is suggested that the conversion of thymine to thymidine is rate-limiting, while the conversions of thymidine to dTMP, and of dTMP to dTDP are more rapid than other steps in the salvage pathway of thymidine nucleotide.
D003561:Cytarabine / Q000494:pharmacology; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D009268:Nalidixic Acid / Q000494:pharmacology; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D013941:Thymine / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism*
D004269:DNA, Bacterial; D013942:Thymine Nucleotides; D003561:Cytarabine; D009268:Nalidixic Acid; D013941:Thymine; D013936:Thymidine