A new principle in biospecific affinity chromatography used for purification of cobalamin-binding proteins.
To avoid using protein-denaturing agents for desorption, when purifying cobalamin-binding protein by biospecific affinity chromatography, an affinity column has been prepared where cobalamin is attached through a temperature-labile linkage to insolubilized 3, 3'-diaminodipropylamine. On desorption the protein is obtained in solution saturated with cobalamin. The method has been used for purification of intrinsic factor and transcobalamin I.
D001798:Blood Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D003959:Diamines; D005750:Gastric Juice / Q000032:analysis; D006801:Humans; D007437:Intrinsic Factor / Q000302:isolation & purification; D008024:Ligands; D011437:Propylamines; D011485:Protein Binding; D012685:Sepharose; D014805:Vitamin B 12 / Q000097:blood*
D001798:Blood Proteins; D003959:Diamines; D008024:Ligands; D011437:Propylamines; D007437:Intrinsic Factor; D012685:Sepharose; D014805:Vitamin B 12