[The sensitivity of direct and indirect methods in immunofluorescence and immunoperoxidase techniques: a comparative study (author's transl)].
A comparative study of the sensitivities of the immunofluorescence and immunoperoxidase techniques was undertaken. The material used was tissue from experimental and human glomerulonephritis. With the direct method, immunoperoxidase was 10 to 30 times more sensitive than immunofluorescence. However, the sensitivities became equivalent if the level of the labeling in immunofluorescence was equal to or superior to 6. In the indirect method, the sensitivity of the immunoperoxidase technique was one to ten-fold greater than with immunofluorescence.
D000818:Animals; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000276:immunology*; D006735:Horseradish Peroxidase / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007668:Kidney / Q000032:analysis; D010544:Peroxidases / Q000276:immunology*; D051381:Rats
D007074:Immunoglobulin G; D006735:Horseradish Peroxidase; D010544:Peroxidases
Comparaison de la sensibilité des techniques d'immunofluorescence et d'immunoperoxydase en méthodes directe et indirecte