title: Effects of flow rate and potassium intake on distal tubular potassium transfer.
abstract: Potassium transport was studied across proximal and distal tubular epithelium in rats on a normal, low- and high-potassium intake during progressive loading with isotonic saline (150 mM) or a moderately hypersomotic urea (200 mM) sodium chloride (100 mM) solution. Free-flow micropuncture and recollection techniques were used during the development of diruesis and tubular fluid (TF) analyzed for inulin-14C, potassium (K) and sodium (Na). Tubular puncture sites were localized by neoprene filling and microdissection. During the large increase in tubular flow rates (10 times): 1) fractional potassium reabsorption fell along the proximal tubule, 2) TFk along the distal tubule remained constant and independent of flow rate in control and high-k rats; thus, net potassium secretion increased in proportion to and was limited by flow rate. 3) In low-K rats TF k fell; with increasing flow rates distal K secretion was not effectively stimulated. 4) Distal tubular sodium reabsorption increased in all animals with flow rate, but tubular Na-K exchange ratios varied greatly. It is suggested that whenever sodium delivery stimulates distal tubular potassium secretion it does so by 1) increasing volume  distal tubular potasssium secretion and by 2) augmenting the transepithelial electrical potential difference (lumen negative).
mesh_terms: D000818:Animals; D001692:Biological Transport; D004032:Diet; D004231:Diuresis; D005919:Glomerular Filtration Rate; D007444:Inulin / Q000378:metabolism; D007684:Kidney Tubules / Q000378:metabolism*; D007686:Kidney Tubules, Distal / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D011188:Potassium / Q000378:metabolism* / Q000494:pharmacology / Q000652:urine; D051381:Rats; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
chemical_list: D012965:Sodium Chloride; D014508:Urea; D007444:Inulin; D012964:Sodium; D011188:Potassium