Evidence that adrenergic nerves are responsible for the active uptake of noradrenaline in the guinea-pig isolated trachea.
1 6-Hydroxydopamine (50 mg/kg, i.p.) was given to guinea-pigs to destroy the adrenergic nerve terminals in the trachea. 2 The destruction was demonstrated by fluorescence histochemistry, which showed a marked loss of beaded fluorescent terminal fibres and by electrical transmural stimulation of the isolated atropinized trachea, which showed a marked reduction of dilator responses. 3 Such tracheae showed greatly reduced uptake-with-retention of (minus)-[3H]-noradrenaline in incubation experiments and the efflux curve of radioactive material showed a selective but incomplete reduction in the volume of the slowly exchanging compartment. 4 It is concluded that much, but perhaps not all, of the uptake-with-retention occurs into adrenergic nerves.
D000818:Animals; D001693:Biological Transport, Active; D004558:Electric Stimulation; D005260:Female; D006168:Guinea Pigs; D006651:Histocytochemistry; D006892:Hydroxydopamines / Q000494:pharmacology; D066298:In Vitro Techniques; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D009638:Norepinephrine / Q000378:metabolism*; D013564:Sympathetic Nervous System / Q000378:metabolism*; D014132:Trachea / Q000378:metabolism*
D006892:Hydroxydopamines; D009638:Norepinephrine