Serum inhibition of in vitro 67Ga binding by L1210 leukemic cells.
The influence of human serum on in vitro 67Ga uptake by L1210 leukemic lymphoblasts has been investigated. Both high- and low-molecular-weight serum components inhibit cellular uptake of the isotope. Inhibition by the high-molecular-weight serum fraction correlates closely with the extent of binding of the radionuclide. Although transferring participates in high-molecular-weight inhibition, it accounts for 10 percent or less of the inhibitory and binding capacity. Similarly, various low-molecular-weight serum components, including citrate, phosphate, glutamate, and others, contribute to inhibition. This inhibition of 67Ca uptake by serum results from the presence of several, perhaps many, inhibitory components.
D000818:Animals; D001769:Blood; D002850:Chromatography, Gel; D002951:Citrates / Q000494:pharmacology; D006224:Cricetinae; D003864:Depression, Chemical; D005708:Gallium / Q000378:metabolism*; D005710:Gallium Radioisotopes; D005971:Glutamates / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007939:Leukemia L1210 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008970:Molecular Weight; D010710:Phosphates / Q000494:pharmacology; D012709:Serum Albumin / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology
D002951:Citrates; D005710:Gallium Radioisotopes; D005971:Glutamates; D010710:Phosphates; D012709:Serum Albumin; D014168:Transferrin; D005708:Gallium