Cytochalasin B: effect on phospholipid metabolism and lysosomal enzyme release by leukocytes;.
The effect of cytochalasin B on phospholipid metabolism and beta-glucuronidase extrusion by polymorphonuclear leukocytes from guinea pid peritoneal exudates has been studied. Cytochalasin B inhibited the engulfing of starch granules by leukocytes, but it enhanced the incorporation of 32-Pi into phosphatidic acid and the phosphoinositidesmit also stimulated the release of beta-glucuronidase into the incubation medium in the presence or absence of starch granulesmkinetic studies showed that the effects of cytochalasin B on 32-Pi incorporation into phosphatidic acid and the phosphoinositides, and the release of beta-glucuronidase into the extracellular medium were comparablempulse-chase experiment revealed that cytochalasin B did not stimulate the isotopic decay of prelabeled lipids, indicating that cytochalasin B increased the radiophosphorus activity of phosphatidic acid and the phosphoinositides by increasing the synthesis of these lipidsmthe incorporation of myo-[2-3H]inositol into the phosphoinositides was also enhanced in the presence of cytochalasin B, but the incorporation of [methyl-14-C] choline into phosphatidylcholine and sphingogonyelin was unchanged;
D000818:Animals; D002794:Choline / Q000378:metabolism; D003571:Cytochalasin B / Q000494:pharmacology*; D005966:Glucuronidase / Q000097:blood; D006168:Guinea Pigs; D007294:Inositol / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010716:Phosphatidylinositols / Q000096:biosynthesis; D010743:Phospholipids / Q000097:blood*; D013109:Sphingomyelins / Q000096:biosynthesis; D013997:Time Factors
D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D010743:Phospholipids; D013109:Sphingomyelins; D003571:Cytochalasin B; D007294:Inositol; D007770:L-Lactate Dehydrogenase; D005966:Glucuronidase; D002794:Choline