Stimulation of the uptake of soluble proteins into isolated HeLa nuclei by pancreatic deoxyribonuclease.
The uptake into isolated HeLa nuclei of radioactive cytosol proteins and purified Escherichia coli ribosomal protein L7 is stimulated up to 4-fold by pancreatic deoxyribonuclease (DNase I). Similar effects are not observed with pancreatic ribonuclease A or phospholipase C. The results reported suggest that there is a general stimulatory effect of DNase on protein uptake by nuclei.
D001426:Bacterial Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000494:pharmacology*; D004996:Ethidium / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D010740:Phospholipases / Q000494:pharmacology; D012260:Ribonucleases / Q000494:pharmacology; D012270:Ribosomes / Q000378:metabolism; D014316:Tritium
D001426:Bacterial Proteins; D009363:Neoplasm Proteins; D014316:Tritium; D003609:Dactinomycin; D003851:Deoxyribonucleases; D010740:Phospholipases; D012260:Ribonucleases; D004996:Ethidium; D007930:Leucine