title: Rifampicin inhibition of the plastid rRNA synthesis of Marchantia polymorpha.
abstract: The effect of rifampicin on the synthesis of plastid rRNA in Marchantia polymorpha was studied in vivo. As bacterial rRNA and plastid rRNA have the same electrophoretic mobilities, this study was possible only after a method for inhibiting bacterial contamination was developed. It was established that 91-100% of the rRNA synthesized by cultures of bacteria from Marchantia, after a labelling period of 3 and 9 h by 32-P, is inhibited by 10 mug/ml of rifampicin. The same inhibition was observed when Marchantia was labelled for 3 h in the presence of 10 mug/ml of rifampicin, showing that no plastid rRNA was synthesized under out conditions, but only bacterial RNA. However, when labelling was continued for 9 h two important peaks of rRNA (23 and 19 s) were labelled in the presence of 10 or 20 mug/ml of rifampicin. These peaks are of chlorophastic origin as confirmed by the following facts: the labelling is light-activated; plastids isolated from  thalli labelled for 12 h also show these two radioactive peaks. Cytoplasmic rRNA is synthesized under certain conditions. The synthesis of plastid rRNA is inhibited by higher concentrations of rifampicin, a concentration of 250 mug/ml producing at least 75% inhibition. Marchantia, a primitive multicellular plant, differs in this respect from higher plants, which seem to be, in most cases, insensitive to rifampicin
mesh_terms: D001419:Bacteria / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010944:Plants / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D012335:RNA, Ribosomal; D012293:Rifampin