The transfer of galactose from UDP-galactose to endogenous lipid acceptors in liver microsomes.
When the microsomal fraction of beef liver is incubated with UDP-[14-C]-galactose in the presence of an inhibitor of nucleotide pyrophosphatase, there is an incorporation of the [14-C]galactose into glycoprotein and into two lipid components, one soluble in chloroform and the other in chloroform/methanol/water (1:1:0.3). Chromatography of the chloroform fraction on DEAE-cellulose or Kieselguhr G gives a single peak with behavior identical to that of dolichol phosphate mannose. Hydrolysis of the chloroform fraction released free galactose. It seems, therefore, that galactose, like glucose, mannose, and N-acetylglucosamine, can be transferred from its respective sugar nucleotide to glycoprotein via dolichol intermediates.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002855:Chromatography, Thin Layer; D004286:Dolichols / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis; D007700:Kinetics; D008358:Mannose; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism; D011955:Receptors, Drug; D013997:Time Factors; D014531:Uridine Diphosphate Galactose / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
D004286:Dolichols; D006023:Glycoproteins; D009943:Organophosphorus Compounds; D011955:Receptors, Drug; D014539:Uridine Diphosphate Sugars; D014531:Uridine Diphosphate Galactose; D000255:Adenosine Triphosphate; D008358:Mannose; D005690:Galactose