Polynucleotides containing 5-mercapto-substituted pyrimidines: inhibition of viral DNA polymerases and the biological implication.
Partially thiolated polycytidylic acids MPC I-III, containing 1.7%, 3.5% and 8.6% 5-mercaptocytidylate units, respectively) inhibited the DNA polymerase of Friend leukemia virus (FLV) in the endogenic reaction as well as in the presence of poly(A)-(dT)14 or poly[d(a-T)] templates; the inhibitory activities were directly related to the percent of thiolation. Various partially thiolated RNA and DNA isolates from Ehrlich ascites cells (containing one 5-mercaptopyrimidine nucleotide/50-100 nucleotide units) also inhibited the DNA polymerases of FLV in the endogenic reaction, and also in the presence of the synthetic templates. The thiolated DNA was the most active, but the thiolated tRNA also showed substantial inhibitory effects, while the thiolated ribosomal RNA was less effective. In a bacterial DNA polymerase (E. coli-K12, using denatured DNA as template), MPC I-III showed no activity. By contrast, MPC III and several partially thiolated nucleic acid isolates significantly inhibited a regenerating rat liver DNA polymerase (I) system; among those tested, the thiolated DNA from Ehrlich ascites cells showed the highest activity. Kinetic analysis of the inhibitory action of this thiolated DNA in the rat liver enzyme system, using as template the corresponding unmodified DNA, demonstrated that the thiolated DNA acts as a competitive inhibitor of the template, with a Ki/Km ratio of 0.5.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D004254:DNA Nucleotidyltransferases / Q000037:antagonists & inhibitors*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D005622:Friend murine leukemia virus / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008115:Liver Regeneration / Q000187:drug effects; D009858:Oncogenic Viruses / Q000187:drug effects / Q000201:enzymology*; D011066:Poly C / Q000031:analogs & derivatives / Q000494:pharmacology*; D011131:Polyribonucleotides / Q000494:pharmacology*; D012334:RNA, Neoplasm / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D051381:Rats; D013045:Species Specificity; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D013698:Templates, Genetic
D011131:Polyribonucleotides; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D013438:Sulfhydryl Compounds; D011066:Poly C; D004254:DNA Nucleotidyltransferases