Globin mRNA from rabbit reticulocyte membrane-bound ribosomes.
Messenger RNA has been isolated from both free and membrane-bound rabbit reticulocyte ribosomes. Membrane-bound ribosomes, which constitute less than 10% of total cellular ribosomes, are released from the membrane by deoxycholate but remain associated with the membrane fraction following treatment with 0.5 M KC1. The major messenger RNA species isolated from either free or bound ribosomes is globin messenger. Both alpha- and beta-globin chains are synthesized when the isolated messenger RNA from either free or membrane-bound reticulocyte ribosomes is added to a Krebs ascites cell-free system.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000097:blood*; D012335:RNA, Ribosomal / Q000097:blood*; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis* / Q000378:metabolism; D012270:Ribosomes / Q000032:analysis* / Q000378:metabolism
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D005914:Globins