Studies of specificity of deoxyribonuclease from salmon testes.
A limited digest of thymus DNA with salmon testes DNase was composed of fragments larger than tetranucleotides. With exhaustive digestion mono-, di-, and trinucleotides were formed. At the (3') omega terminus G predominated, its frequency ranged from approximately 35 to 72% and increased with decreasing size of fragments. The error caused by the ribose-containing contaminants of DNA is significant, and should not be neglected in the evaluation of nucleoside frequency at the terminal positions of fragments.
D000818:Animals; D002417:Cattle; D004247:DNA; D003851:Deoxyribonucleases / Q000378:metabolism*; D008297:Male; D009841:Oligonucleotides / Q000032:analysis / Q000302:isolation & purification; D012474:Salmon; D013056:Spectrophotometry, Ultraviolet; D013737:Testis / Q000201:enzymology*; D013950:Thymus Gland
D009841:Oligonucleotides; D004247:DNA; D003851:Deoxyribonucleases