[Synthesis and study of the properties of water-insoluble products of the interaction of urease and cellulose derivatives].
As a result of reaction of urease with graft copolymer of cellulose and polyglycidyle methacrylate or with carboxymethyl cellulose, products were synthesized, containing about 2% of chemically bound urease. Binding with the cellulose derivatives was accompanied by about two-fold decrease in urease activity. Carboxymethyl cellulose-urease and polyglycidile methacrylate-cellulose-urease might be repeatedly (for 30 cycles) used for hydrolysis of urea; the enzymatic activity of the first compound did not change, but of the second one--was decreased by 30%. Activity of the compounds was not changed after storage within 2 months in 0.1 M phosphate buffer, pH 7.0 at 4 degrees C.
D000179:Acrylates / Q000138:chemical synthesis; D002266:Carboxymethylcellulose Sodium / Q000138:chemical synthesis / Q000494:pharmacology; D002482:Cellulose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D004347:Drug Interactions; D004356:Drug Storage; D012995:Solubility; D013997:Time Factors; D014510:Urease / Q000032:analysis* / Q000138:chemical synthesis / Q000494:pharmacology; D014867:Water
D000179:Acrylates; D014867:Water; D002482:Cellulose; D014510:Urease; D002266:Carboxymethylcellulose Sodium
Sintez i issledovanie svoĭstv nerastvorimykh v vode produktov vzaimodeĭstviia ureazy i proizvodnykh tselliulozy