Electrophysiological effects of burimamide and 16,16-dimethyl prostaglandin E2 on the canine gastric mucosa.
The electrophysiological effects of two potent inhibitors of gastric acid secretion, burimamide and 16,16-dimethyl prostaglandin E2 (dm-PGE2), were determined in an in vivo histamine-stimulated canine stomach preparation and an in vitro canine gastric mucosal preparation. In the in vivo stomach preparation, intravenous burimamide caused a decrease in acid secretion, an increase in transmucosal potential difference (PD) and the relative resistance (R) was essentially unchanged. Intravenous dm-PGE2 also inhibited acid secretion and increased PD but, in contrast to burimamide, increased R. In the in vitro preparation, the unidirectional flux of sodium from mucosa to serosa increased after dm-PGE2 but not after burimamide. Passive sodium fluxes and unidirectional chloride fluxes were not altered after either agent. These findings suggest that increased active transport of sodium from mucosa to serosa is at least partially responsible for the observed increase in transmural PD with dm-PGE2, an agent which also decreases hydrogen ion transport. With burimamide the increased PD was due primarily to inhibition of hydrogen ion secretion.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002049:Burimamide / Q000494:pharmacology*; D002712:Chlorides / Q000378:metabolism; D002713:Chlorine; D004285:Dogs; D004594:Electrophysiology; D005260:Female; D005750:Gastric Juice / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D011458:Prostaglandins E / Q000494:pharmacology*; D011868:Radioisotopes; D012964:Sodium / Q000378:metabolism; D012979:Sodium Radioisotopes; D013890:Thiourea / Q000031:analogs & derivatives*
D002712:Chlorides; D011458:Prostaglandins E; D011868:Radioisotopes; D012979:Sodium Radioisotopes; D002713:Chlorine; D006632:Histamine; D012964:Sodium; D013890:Thiourea; D002049:Burimamide