title: Content of elongation factor Tu in Escherichia coli.
abstract: The content of elongation factor Tu in E. coli B has been determined both by radioimmune assay and by GDP binding. The two assays gave comparable results: cells growing at 2 doublings per hour contained about 8 molecules of Tu per ribosome, whereas those growing at 0.22 doublings per hour contained about 14 molecules per ribosome. These levels resemble those reported for tRNA, in contrast with the 1:1 ratio of factor to ribosomes reported for elongation factors Ts and G.
mesh_terms: D000085:Acetates / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002455:Cell Division; D004926:Escherichia coli / Q000032:analysis* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006150:Guanine Nucleotides / Q000378:metabolism; D007700:Kinetics; D010445:Peptide Elongation Factors / Q000032:analysis*; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012210:Rhamnose / Q000378:metabolism
chemical_list: D000085:Acetates; D006150:Guanine Nucleotides; D010445:Peptide Elongation Factors; D005947:Glucose; D012210:Rhamnose