Turnover of polyadenylate-containing ribonucleic acid in Saccharomyces cerevisiae.
We examined the kinetics of incorporation of [3H]adenine into polyadenylate-containing ribonucleic acid [poly(A)-containing RNA] in yeast. The total poly(A)-containing RNA from spheroplasts and intact cells and the polysomal poly(A)-containing RNA exhibited similar incorporation kinetics. At 30 C half-saturation of the pool of poly(A)-containing RNA with label occurred in approximately 22 min. Since precursor pools appeared to require 5 min to saturate with label, we conclude that at 30 C messenger RNA molecules in yeast decay with an average half-life of 17 min.
D000225:Adenine / Q000378:metabolism; D007700:Kinetics; D009694:Nucleic Acid Precursors / Q000378:metabolism; D011061:Poly A / Q000096:biosynthesis; D011132:Polyribosomes / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013104:Spheroplasts / Q000378:metabolism; D013696:Temperature
D009694:Nucleic Acid Precursors; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A; D012313:RNA; D000225:Adenine