Effect of intrauterine copper on the nucleic acids, polysome pattern, and glycoprotein composition of the human endometrium.
The modifications induced by the intrauterine release of copper in the macromolecular and glycoprotein composition of the proliferative and secretory human endometrium were studied. In addition, the endometrial changes produced in women users of copper-T intrauterine devices in the polysome pattern and in the content of ribonucleoprotein particles were determined during the secretory phase. A group of ten untreated normal women (control group) and 15 users of 200 mm.-2 copper-T intrauterine device were selected for this study from the outpatient clinic of the Hospital de Ginecologia y Obstetricia No. 2 del Instituto Mexicano del Seguro Social. The main changes observed in the copper-T users were: a significant decrease in the endometrial content of RNA in both phases of the menstrual cycle, a significant decrease in protein in the secretory phase, and drastic changes in the fucose-sialic acid ratios, which decreased during the proliferative and increased during the secretory phases. Normal human secretory endometrium contained 4.89 plus os minus 0.28 (mean plus or minus standard error (S. E.) mg. ribonucleoprotein particle per gram wet weight, while the endometrium of the Cu-T users showed a significant decrease to 2.52 plus or minus 0.17 (mean plus or minus S. E.). In addition, the Cu-T induced a decrease in the heavy components of the polysome pattern with a concomitant increase in the lighter components.
D000328:Adult; D002499:Centrifugation, Density Gradient; D003300:Copper / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D004717:Endometrium / Q000032:analysis* / Q000166:cytology / Q000187:drug effects; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007434:Intrauterine Devices; D009698:Nucleoproteins / Q000032:analysis; D011132:Polyribosomes / Q000187:drug effects*; D011247:Pregnancy; D012313:RNA / Q000032:analysis; D012265:Ribonucleotides / Q000032:analysis; D013395:Sucrose
D006023:Glycoproteins; D009698:Nucleoproteins; D012265:Ribonucleotides; D013395:Sucrose; D012313:RNA; D003300:Copper; D004247:DNA