Quantitative studies of pinocytosis. I. Kinetics of uptake of (125I)polyvinylpyrrolidone by rat yolk sac cultured in vitro.
A method is described for the in vitro culture of 17.5-day rat visceral yolk sac. Tissue survival was good as judged by light and electron microscopy. The rate of pinocytic uptake of 125I-labeled polyvinylpyrrolidone by the tissue was constant both within and between experiments. Within the concentration range 0.15-24 mug/ml, the 125I-labeled polyvinylpyrrolidone neither stimulated nor inhibited pinocytosis. The system offers many advantages in the quantitative study of the physical basis of pinocytosis.
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D007457:Iodine Radioisotopes; D008854:Microscopy, Electron; D010873:Pinocytosis; D011205:Povidone / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D012044:Regression Analysis; D013997:Time Factors; D014817:Vitelline Membrane / Q000378:metabolism* / Q000648:ultrastructure
D007457:Iodine Radioisotopes; D011205:Povidone