title: Simultaneous high-speed liquid chromatographic determination of tetracycline and rolitetracycline in rolitetracycline formulations.
abstract: A rapid, precise high-speed liquid chromatographic procedure for the simultaneous determination of tetracycline and rolitetracycline in rolitetracycline formulations is described. Samples are dissolved in water, chilled to 0 degrees, and chromatographed on a pellicular cation-exchange resin, The specificity of this method represents a significant improvement over present analytical procedures, which fail to differentiate between rolitetracycline and its hydrolysis product, tetracycline, in these formulations.
mesh_terms: D001681:Biological Assay; D002852:Chromatography, Ion Exchange / Q000379:methods; D009566:Nitrates / Q000032:analysis; D012382:Rolitetracycline / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013752:Tetracycline / Q000032:analysis*; D013754:Tetracyclines; D013997:Time Factors
chemical_list: D009566:Nitrates; D013754:Tetracyclines; D013752:Tetracycline; D012382:Rolitetracycline