title: Characterization of a new infectious bronchitis virus isolate. III. Cell-culture adaptation of Clark 333.
abstract: The Clark 333 strain of infectious bronchitis virus (IBV) was substantially resistant to primary chicken cell-culture adaptation. More than 40 serial embryo passages were required before the virus would produce cytopathic alterations upon cell-culture inoculation. The cytopathic effect was characteristic of the effect of reported for IBV. Adaptation was not accomplished by alternating serial passages in embryo and cell-cultured systems. A careful monitoring of cell-culture fluid infectivity by embryo inoculation was found to be essential because absence of adaptation is accompanied by a loss in virus titer (3 to 5 serial passages). Helpful additional aids were immunofluorescence and cytopathology.
mesh_terms: D000818:Animals; D001991:Bronchitis / Q000382:microbiology / Q000662:veterinary*; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003332:Coronaviridae / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D007668:Kidney; D011201:Poultry Diseases / Q000382:microbiology*; D014777:Virus Diseases / Q000382:microbiology / Q000662:veterinary*; D014779:Virus Replication
chemical_list: D003470:Culture Media