Development of a micromethod for identification of anaerobic bacteria.
A microprocedure was described for determining the carbohydrate fermentation patterns of 48 anaerobic bacteria at one time in microtiter plates. The cultures were transferred into agar-filled wells of microtiter plates with a replicator inside an anaerobic glove box. Fermentation was measured both with a colorimetric indicator and with a small pH electrode. The method was approximately 97% accurate. It would be most useful for laboratories that need to  identify large numbers of anaerobes at one time.
D000693:Anaerobiosis; D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D001431:Bacteriological Techniques / Q000295:instrumentation; D050260:Carbohydrate Metabolism; D003470:Culture Media; D003545:Cysteine / Q000494:pharmacology; D004226:Dithioerythritol / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D005285:Fermentation; D005557:Formaldehyde / Q000494:pharmacology; D008623:Mercaptoethanol / Q000494:pharmacology
D003470:Culture Media; D005557:Formaldehyde; D008623:Mercaptoethanol; D004226:Dithioerythritol; D003545:Cysteine