[The effect of fumaric acid monoethylester on the synthesis of nucleic acids and proteins of PHA-stimulated human lymphocytes (author's transl)].
Fumaric acid monoethylester (FAME) inhibits the incorporation of 14C-Thymidin, 14C-Uridin, 14C-Alanin and 14C-Leucin into acid-insoluble biopolymers of cultivated PHA-stimulated human lymphocytes. At high concentrations of FAME (500 mug/ml culture medium) the inhibition of nucleic acid synthesis is 6 times higher on the average than the inhibition of protein synthesis. However, the application of the cis-isomer, maleic acid monoethylester (MAME), results in an increase of the incorporation rate of the labelled precursors into the RNA and DNA. This is 3.5--9.3 times higher than after application of FAME. The results demonstrate the specific inhibition by FAME. The rate of labelling of nucleic acids is decreased above 10 mug FAME/ml culture medium and in the case of MAME above 50 mug/ml medium. As an explanation of the specific action of FAME its influence on the enzymes of the nucleic acid synthesis, the citric acid cycle or a faulty synthesis of enzymes are discussed.
D000409:Alanine / Q000378:metabolism; D002250:Carbon Radioisotopes; D002952:Citric Acid Cycle; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004952:Esters; D005650:Fumarates / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D008298:Maleates / Q000494:pharmacology; D009696:Nucleic Acids / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D002250:Carbon Radioisotopes; D004952:Esters; D005650:Fumarates; D037102:Lectins; D008298:Maleates; D009696:Nucleic Acids; D012313:RNA; D004247:DNA; D007930:Leucine; D000409:Alanine; D013936:Thymidine; D014529:Uridine
Der Einfluss von Fumarsäuremonoäthylester auf die Nucleinsäure- und Proteinsynthese PHA-stimulierter menschlicher Lymphocyten