Improved microscale assay for purine phosphoribosyltransferase activities.
We describe an improved and rapid filter-type assay for purine phosphoribosyltransferase activities. 14C-labeled purine bases are used as substrates, and the equipment includes a sampling manifold and glass-fiber filters coated with polyethyleneimine-cellulose. The method is especially suited for assay of a large number of samples, and may be useful for other enzyme activity measurements.
D000228:Adenine Phosphoribosyltransferase / Q000032:analysis*; D004912:Erythrocytes / Q000201:enzymology; D005374:Filtration; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis*; D008722:Methods; D010430:Pentosyltransferases / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase