Bile acids. XLIV, quantitation of bile acids from the bile fistula rat given (4-14C) cholesterol.
The bile acids derived from [4-14-C]cholesterol administered intracardially to rats with cannulated bile ducts were identified and quantitated. Over a period of 28 days about 90% of the administered 14-C was found in bile of which 73% was retained in the biliary acid fraction. [7beta-3-H]cholic acid, alpha-muri[3beta-3-H]cholic acid, beta-muri[3beta-3-H]cholic acid and litho[3beta-3-H]cholic acid were prepared with specific activities of about 30 muCi/mg by reduction of appropriate ketonic precursors with NaB3H4 and were added to the biliary acid fraction. After separation and purification of the bile acids, cholic, chenodeoxycholic, alpha- and beta-muricholic acids accounted for 70, 16, 7.5 and 6.1%, respectively, of the 14-C in the biliary acid fraction. The specific activities of these isolated 14-C-labeled acids were almost identical. Lithocholic acid accounted for a maximum of 0.2% and ursodeoxycholic acid and 7-oxolithocholic acid could account for no more than 2% of the biliary 14-C. Gas-liquid chromatography on 3% OV-17 of the trimethylsilyl ether derivatives of the methyl esters of the common bile acids of rat bile results in their complete separation and provides a convenient means of estimating the relative proportions of these acids in rat bile. By this method, the relative amounts of the four major acids, cholic, chenodeoxycholic, alpha- and beta-muricholic acids were 63, 20, 8 and 6%, respectively.
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism* / Q000652:urine; D001652:Bile Ducts; D002250:Carbon Radioisotopes; D002404:Catheterization; D002635:Chenodeoxycholic Acid / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D002793:Cholic Acids / Q000031:analogs & derivatives / Q000378:metabolism; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003840:Deoxycholic Acid / Q000378:metabolism; D008095:Lithocholic Acid / Q000378:metabolism; D008297:Male; D051381:Rats; D013997:Time Factors
D001647:Bile Acids and Salts; D002250:Carbon Radioisotopes; D002793:Cholic Acids; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; C004821:muricholic acid; D008095:Lithocholic Acid; D002784:Cholesterol