Effect of insulin and glucose on the glucose consumption and glycogen synthesis by the rat diaphragm.
Insulin activated both the uptake of glucose-C-14 from the medium and its incorporation into glycogen by the incubated diaphragm: the percentage of glucose-C-14 incorporated into glycogen increased with an increase in the dose of insulin. An increase in the concentration of glucose in the medium also caused this more rapid assimilation by the muscle tissue. However significant stimulation of glycogen synthesis was observed only in the presence of a high (unphysiological) glucose concentration in the incubation fluid; the percentage of glucose-C-14 incorporated into glycogen remained small and constant. It is postulated that the glucose-C-14 found in the case of substrate control is the result of exchange of the glucose residues of glycogen with intracellular glucose.
D000818:Animals; D003964:Diaphragm; D005260:Female; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D006003:Glycogen / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D009132:Muscles / Q000378:metabolism*; D051381:Rats; D013268:Stimulation, Chemical
D007328:Insulin; D006003:Glycogen; D005947:Glucose