Direct selection of mutants restricting efficiency of suppression and misreading levels in E. coli B.
We describe a method for the direct selection of E. coli mutants restricting efficiency of suppression and misreading levels using a T4-coded nonsense suppressor. One mutant isolated has the phenotype expected for a restrictive mutant and may be ribosomal. Other possibilities are discussed.
D001435:Bacteriophages; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000302:isolation & purification; D005838:Genotype; D009154:Mutation; D010641:Phenotype; D013489:Suppression, Genetic; D014158:Transcription, Genetic