Micro-radioimmunoassay for antibodies to Newcastle disease virus in the chicken.
An indirect micro-radioimmunoassay is described in which chicken anti-Newcastle disease virus antibody was detected, with radioactively labeled rabbit anti-chicken Fab, on virus-infected microcultures of chick embryo fibroblasts. Newcastle disease virus-infected microcultures were formalin fixed and stored at 4 degrees C for up to 4 months without affecting the sensitivity of the test. The micro-technique was found to be highly sensitive and specific assay of anti-viral antibody and may allow detection of immunoglobulin class of anti-Newcastle disease virus antibody.
D000361:Agammaglobulinemia / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001991:Bronchitis / Q000276:immunology; D002060:Bursa of Fabricius / Q000276:immunology / Q000528:radiation effects; D002478:Cells, Cultured; D002645:Chickens; D003588:Cytopathogenic Effect, Viral; D004531:Eggs; D005347:Fibroblasts / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006564:Herpesviridae / Q000276:immunology; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D009522:Newcastle disease virus / Q000276:immunology*; D011309:Preservation, Biological; D011817:Rabbits / Q000276:immunology; D011830:Radiation Effects; D011863:Radioimmunoassay / Q000379:methods*; D014776:Virus Cultivation
D000914:Antibodies, Viral; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes