The effect of diamide and glutathione on the uptake of alpha-methyl-D-glucoside by slices of rat kidney cortex.
The uptake of alpha-methyl-D-glucoside was stimulated in slices of rat kidney cortex by pretreatment with reduced glutathione. Diamide, an oxidizind agent with high specificity for GSH, caused an inhibition of alpha-methyl-D-glucoside uptake. These effects appeared to be related specifically to GSH, since dithiothreitol and mercaptoethanol did not increase alpha-methyl-D-glucoside uptake, and were not as effective as GSH in reversing the effects of diamide. GSH and diamide had no effect on the uptake of another sugar analog, 3-O-methylglucose, which is not actively transported. Kinetic studies indicated that GSH increased the apparent V without affecting K-m. The results are discussed in terms of the possible role of GSH in the process of sugar transport.
D000577:Amides / Q000494:pharmacology*; D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D001692:Biological Transport; D004229:Dithiothreitol / Q000494:pharmacology; D005260:Female; D005559:Formamides / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology*; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism*; D007700:Kinetics; D008623:Mercaptoethanol / Q000494:pharmacology; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D051381:Rats
D000577:Amides; D001391:Azo Compounds; D005559:Formamides; D008757:Methylglucosides; D008759:Methylglycosides; D008623:Mercaptoethanol; D005978:Glutathione; D005947:Glucose; D004229:Dithiothreitol