Biosynthesis of galactosyl diglycerides by non-green fractions from chloroplasts.
When either mitochondria, chloroplast stroma lamellae, or osmotically shocked chloroplasts were centrifuged through sucrose gradients, zones were always obtained at the 0.6 M-0.9 M boundary which were highly active in galactosyltransferase. These activities did not coincide with maxima for chlorophyll or cytochrome c oxidase activity. A second chlorophyll-free fraction was obtained at lower density, showing high galactosyltransferase activity when incubated after isolation. The results indicate that the highly active fractions originate from chloroplast envelopes.
D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000201:enzymology / Q000378:metabolism*; D004075:Diglycerides / Q000096:biosynthesis*; D005690:Galactose / Q000378:metabolism*; D005989:Glycerides / Q000096:biosynthesis*; D006602:Hexosyltransferases / Q000378:metabolism; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D010944:Plants; D013997:Time Factors
D004075:Diglycerides; D005989:Glycerides; D002734:Chlorophyll; D006602:Hexosyltransferases; D005690:Galactose