Genetic analysis of adenovirus type 2. I. Isolation and genetic characterization of temperature-sensitive mutants.
Temperature-sensitive mutants which replicate normally at 33 C but poorly at 39 C were isolated from nitrosoguanidine- or nitrous acid-mutagenized adenovirus 2 by (i) testing the cytopathic effect or inclusion body-forming capacity of random plaque isolates, or (ii) reduced plaque enlargement upon shifting from 33 to 39 C. Thirty-six mutants were isolated with 33 C/39 C plaque ratios varying from 20 to 10-5. Some of these mutants could be arranged into 13 groups by the complementation test. By means of recombination analysis a provisional linear genetic map was constructed.
D000256:Adenoviridae / Q000145:classification / Q000254:growth & development*; D002277:Carcinoma; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D003433:Crosses, Genetic; D003588:Cytopathogenic Effect, Viral; D005796:Genes; D005816:Genetic Complementation Test; D006801:Humans; D007181:Inclusion Bodies, Viral; D007822:Laryngeal Neoplasms; D009062:Mouth Neoplasms; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D009608:Nitrous Acid; D011995:Recombination, Genetic; D013696:Temperature; D010948:Viral Plaque Assay; D014779:Virus Replication
D009153:Mutagens; D009604:Nitrosoguanidines; D009608:Nitrous Acid