Carbon tetrachloride induced polysome breakdown. Relative importance of lipid peroxidation and of binding to ribosomal components in the process.
In vivo, CC1-4 causes an intense polysome breakdown; CHC1-3 effect is much less intense and CH2C1-2 effect is negligible. Previous administration of either diphenyl-p-phenyelnediamine or alpha-tocopherol to the rats does not prevent CC1-4-induced polysome breakdown. Promethazine and cystamine were able to prevent to a good extent the CC1-4 induced polysome breakdown process. 14CC1-4 irreversibly binds to ribosomal proteins but not to ribosomal RNA. Several hepatocarcinogens which are effective alkylating agents and cause in vivo polysome breakdown do not induce a lipid peroxidation process.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002251:Carbon Tetrachloride / Q000378:metabolism / Q000494:pharmacology*; D003538:Cystamine / Q000494:pharmacology; D005609:Free Radicals; D047348:Hydrolyzable Tannins / Q000494:pharmacology; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D010545:Peroxides / Q000378:metabolism; D010655:Phenylenediamines / Q000494:pharmacology; D011132:Polyribosomes / Q000187:drug effects*; D011398:Promethazine / Q000494:pharmacology; D051381:Rats; D012270:Ribosomes / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D002250:Carbon Radioisotopes; D005609:Free Radicals; D047348:Hydrolyzable Tannins; D010545:Peroxides; D010655:Phenylenediamines; D014810:Vitamin E; D002251:Carbon Tetrachloride; D011398:Promethazine; D003538:Cystamine