title: Analysis of temperature-sensitive recB and recC mutations.
abstract: The in vivo pleiotropic effects associated with the temperature-sensitive recB270 and recC271 mutations have been correlated with the in vitro behavior of the recBC nucleases coded for by these alleles. The ATP-dependent breakdown of double-stranded DNA is essential for cell viability, radiation repair, and genetic recombination. Temperature sensitivity can be suppressed in vitro and in vivo.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000483:Alleles; D002470:Cell Survival; D004269:DNA, Bacterial / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005092:Exonucleases / Q000378:metabolism; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D011830:Radiation Effects; D011995:Recombination, Genetic; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D008937:Mitomycins; D000255:Adenosine Triphosphate; D003851:Deoxyribonucleases; D004720:Endonucleases; D005092:Exonucleases