Selective damage to erythroblasts by 55-Fe.
The low energy and short range of 55-Fe Auger electrons were utilized in mice to deliver lethal intracellular radiation to iron-incorporating erythropoietic precursors with minimal radiation damage to other bone marrow cells. The ensuing intramedullary, selective erythropoietic death was demonstrated by absolute and differential bone marrow cell counts and by decreased blood uptake of 59-Fe. The decreased number of colony-forming units in spleen colony assay and the decreased ability of tranplanted bone marrow to protect fatally irradiated mice shows that the bone marrow was partially depleted of pluripotent stem cells. These data are interpreted to indicate an increased pluripotent stem cell utilization in response to increased demand for differentiation of stem cells along the erythropoietic pathway.
D000818:Animals; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000528:radiation effects*; D004920:Erythropoiesis / Q000528:radiation effects; D005260:Female; D006412:Hematopoietic Stem Cells / Q000528:radiation effects*; D007501:Iron / Q000378:metabolism; D007504:Iron Radioisotopes; D051379:Mice; D011828:Radiation Chimera; D011830:Radiation Effects; D013154:Spleen / Q000166:cytology; D014184:Transplantation, Homologous
D007504:Iron Radioisotopes; D007501:Iron