[Application of the immunoperoxidase method to the study of the membrane of human lymphocytes].
Immunoelectron microscopy was applied to human lymphocytes exposed to purified peroxidase-conjugated anti-immunoglobulin. Indeed, detectable surface immunoglobulins are a salient feature of so-called " B " lymphocytes. On living cells, a rapid and massive internatization of the labelled membrane is observed. Prior glutaraldehyde cell fixation avoids such a phenomenon. Thus, exposure of fixed cells to conjugated anti-immunoglobulin allows the visualization of a dense and continuous specific membrane labelling. Immunoglobulin-bearing lymphocytes have a characteristic membrane surrounded with numerous microvilli. On the other hand, non-labelled lymphocytes have a smooth membrane. Intermediate forms are also noted among these extreme morphological features.
D000328:Adult; D000888:Antibodies, Anti-Idiotypic; D001402:B-Lymphocytes / Q000648:ultrastructure*; D002462:Cell Membrane / Q000648:ultrastructure*; D006651:Histocytochemistry; D006801:Humans; D010544:Peroxidases; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*
D000888:Antibodies, Anti-Idiotypic; D011947:Receptors, Antigen, B-Cell; D010544:Peroxidases
Application de la méthode de l'immunoperoxydase a l'étude de la membrane des lymphocytes humains