Quantitative extraction of tubocurarine, gallamine, and decamethonium from biological materials.
A procedure is described for quantitative extraction of tubocurarine, gallamine, and decamethonium from blood, urine, and tissue. Ion pairs of the drugs are formed with picric acid and are extracted into a dichloromethane/1-pentanol solvent. Qualitative analysis of the extract is effected using thin-layer chromatography. Quantitative analysis is accomplished by elution of TLC areas containing the drug, formation of the ion pair with sodium 9,10-dimethoxyanthracene-2-sulfonate, and analysis of the ion pair by spectrophotofluorometry.
D000873:Anthracenes; D001965:Bromides / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D003650:Decamethonium Compounds / Q000302:isolation & purification*; D005554:Forensic Medicine; D005703:Gallamine Triethiodide / Q000302:isolation & purification*; D006801:Humans; D008099:Liver / Q000032:analysis; D008168:Lung / Q000032:analysis; D009132:Muscles / Q000032:analysis; D010851:Picrates; D013050:Spectrometry, Fluorescence; D014403:Tubocurarine / Q000302:isolation & purification*
D000873:Anthracenes; D001965:Bromides; D003650:Decamethonium Compounds; D010851:Picrates; D005703:Gallamine Triethiodide; D014403:Tubocurarine