[Fractionation of staphyococcal allergens on sephadex].
Allergens obtained from seven strains of staphylococcus cultures (with and without any toxigenic and hemolytic properties) were subjected to fractionation on Sephadexes. It was shown that allergens from strains 209, 3a, 977 contained one protein-polysaccharide, component with molecular weight of 600 000--900 000. Allergens from strains Wood-46, 150a, r-7, O15 were characterized by the presence of two protein-polysaccharide fractions with molecular weight of 800 000--900 000 (I) and 20 000--100 000 (II). There was revealed the influence of lyphilization on the separation of nucleic acids in the preparations not subjected to lyophilization; nucleic acid fractions were separated in gel filtration; no separation of nucleic acids from the protein-polysaccharide fractions followed lyophilization.
D000485:Allergens / Q000302:isolation & purification; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D003124:Colorimetry; D005612:Freeze Drying; D008970:Molecular Weight; D009584:Nitrogen; D009696:Nucleic Acids; D011135:Polysaccharides, Bacterial; D013210:Staphylococcus / Q000276:immunology*
D000485:Allergens; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D009696:Nucleic Acids; D011135:Polysaccharides, Bacterial; D009584:Nitrogen
Fraktsionirovanie stafilokokkovykh allergenov na sefadeksakh