In vitro reconstitution of anti-sheep erythrocyte antibody response of T cell-depleted spleen cells by allogeneic T cells or by factors derived from them.
Restoration of the impaired antibody response to sheep erythrocytes (SRBC) in cultures of mouse spleen cells, which were deprived of thymus-derived lymphocytes (T cells) by treatment with anti-mouse brain-associated theta (BA theta) antiserum and complement, was studied by adding a small portion of syngeneic or allogeneic normal spleen cells in vitro. Allogeneic spleen cells had a far greater effect than syngeneic spleen cells on the restoration, as far as the normal spleen cells added were able to recognize the alloantigens on the anti-BA theta serum-treated spleen cells (bone marrow-derived lymphocytes). Treatment of the allogeneic spleen cells with mitomycin C did not affect their activity in the restoration of the impaired antibody response. The possibility that the role of T cells in the antibody response to SRBC may be replaced by a nonspecific mediator derived from T cells reacting with allogeneic cells was proven by the finding that supernatant of the mixed allogeneic spleen cell cultures restored the impaired anti-SRBC antibody response of the T cell-depleted spleen cells. The effect of such culture supernatant on the restoration of the antibody response was greatest when it was added to the T cell-depleted spleen cell cultures one day after cultivation with SRBC, suggesting that the effectiveness may result from triggering of the proliferation and differentiation of antibody-forming cell precursors, which have already reacted with the antigen, to antibody-forming cells.
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000187:drug effects / Q000276:immunology; D001921:Brain / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D007106:Immune Sera; D066298:In Vitro Techniques; D007519:Isoantigens; D051379:Mice; D008809:Mice, Inbred C3H; D008937:Mitomycins / Q000494:pharmacology; D012756:Sheep / Q000276:immunology; D016038:Skin Transplantation; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
D007106:Immune Sera; D007519:Isoantigens; D008937:Mitomycins