A direct radioimmunoassay of corticosterone in rat serum.
A direct radioimmunoassay of corticosterone is described requiring as little as 1-2 mul of rat serum. After methanol inactivation of CBG, serum was assayed with a relatively specific antibody raised against corticosterone-21-hemisuccinate. While this antibody has a significant cross-reaction with cortisol, prior purification is not necessary since cortisol is not produced in the rat.
D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D003345:Corticosterone / Q000097:blood*; D003429:Cross Reactions; D006854:Hydrocortisone / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D012712:Serum Globulins
D012712:Serum Globulins; D003345:Corticosterone; D006854:Hydrocortisone