Association of the long-acting thyroid stimulator protector with the immunoglobulin G fraction of serum from patients with thyrotoxicosis.
Both the long-acting thyroid stimulator and the long-acting thyroid stimulator protector are associated with the immunoglobulin G fraction of human serum proteins. Fractionation of globulins by (NH4)2SO4 precipitation and DEAE-cellulose chromatography showed that the protector activity was associated with subfractions of differing electrophoretic mobility. Attempts to concentrate the protector in immunochemically pure immunoglobulin G prepared in this way may fail because of loss of protector activity with other immunoglobulin G fractions. Gel filtration on Sephadex G-150 indicated that protector activity was associated with 7-S proteins.
D000818:Animals; D001665:Binding Sites; D001681:Biological Assay; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D006801:Humans; D006980:Hyperthyroidism / Q000276:immunology*; D007074:Immunoglobulin G / Q000302:isolation & purification; D008135:Long-Acting Thyroid Stimulator; D051379:Mice; D011485:Protein Binding
D007074:Immunoglobulin G; D008135:Long-Acting Thyroid Stimulator