Direct association of messenger RNA with microsomal membranes in human diploid fibroblasts.
Messenger RNA (mRNA) of membrane-bound polysomes in a membrane fraction of WI-38 cells remains associated with the microsomal membranes even after ribosomes and their nascent polypeptide chains are removed by using puromycin in a high salt buffer or by disassembling the ribosomes in a medium of high ionic strength lacking magnesium. mRNA either was specifically labeled in the presence of actinomycin D, or it was recognized by virtue of its affinity for oligo-dT. Poly A segments in bound mRNAs have an electrophoretic mobility in acrylamide gels which is characteristic of cytoplasmic mRNAs and corresponds to 150-200 adenyl residues. Extensive RNase treatment did not lead to release of the poly A segments of membrane-associated mRNA molecules either from an intact membrane fraction or from a membrane fraction previously stripped of ribosomes. On the other hand, RNase treatment led to the release and digestion of the nonpoly A segments of the mRNA molecules, indicating that the site of attachment of mRNA to the ER membranes is located near or at the 3' end of the molecule which contains the poly A. A direct association of mRNAs and endoplasmic reticulum membranes is considered in a modelto explain the assembly of bound polysomes and protein synthesis in a membrane-associated apparatus.
D000249:Adenosine Monophosphate / Q000378:metabolism; D002458:Cell Fractionation; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002845:Chromatography / Q000379:methods; D004171:Diploidy; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling; D008168:Lung / Q000196:embryology; D008566:Membranes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D008961:Models, Structural; D009693:Nucleic Acid Hybridization; D011072:Poly U; D011119:Polynucleotides / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal; D012270:Ribosomes / Q000378:metabolism
D011119:Polynucleotides; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011072:Poly U; D000249:Adenosine Monophosphate