Routine radioimmunoassay of plasma testosterone, and results for various endocrine disorders.
We describe a modification of published methods for radioimmunoassay of plasma testosterone. This simpler method involves no chromatographic steps, and the necessary reagents, including tritiated testosterone and testosterone anti-serum, are commercially available. A digital computer is used for the calculation. Without difficulty, a technician can complete 100 assays in three working days. Mean testosterone concentrations (plus or minus 2 SD) in the plasmas of 21 normal men and 26 women with a normal menstrual cycle were 684 plus or minus 300 and 45 plus or minus 20 ng/dl, respectively. Within- and between-assay precision (coefficient of variation) were 5.2% (n = 29) and 6.7% (n = 26), respectively. We have assayed more than 1000 samples during the past year. We give data on the concentrations of testosterone in plasma of patients with various endocrine disorders.
D000328:Adult; D004700:Endocrine System Diseases / Q000097:blood / Q000175:diagnosis*; D005260:Female; D006628:Hirsutism / Q000097:blood; D006801:Humans; D007018:Hypopituitarism / Q000097:blood; D008297:Male; D008722:Methods; D010911:Pituitary Neoplasms / Q000097:blood; D011247:Pregnancy; D011863:Radioimmunoassay; D013739:Testosterone / Q000097:blood*; D014316:Tritium
D014316:Tritium; D013739:Testosterone