Effect of triphenylmethane derivatives on cell-free macromolecular synthesis. II. mRNA-ribosome binding.
The specific inhibition by aurintricarboxylic acid (ATA) of mRNA binding to ribosomes was re-examined. Among triphenylmethane compounds, all of which are potent inhibitors for phenylalanyl=tRNA synthetase, ATA is the most potent inhibitor. Two other dyes, Aurine and Azure Blue B, inhibit the reaction, but the concentration for 50% inhibition is far higher than that for ATA. It appears that the presence of the carboxyl group on the skeletal-triphenylmethane structure enhances the inhibition of the mRNA-binding reactionmthis feature is slightly different from that of the phenylalanyl-tRNA synthetase reaction, in which the skeletal structure of the dye itself is essential to its inhibitory action.
D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D001665:Binding Sites; D002846:Chromatography, Affinity; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology*; D007700:Kinetics; D010636:Phenols / Q000494:pharmacology; D011072:Poly U / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*
D001565:Benzoates; D003509:Cyclohexanecarboxylic Acids; D010636:Phenols; D012333:RNA, Messenger; D011072:Poly U