Prostaglandin synthesis by bovine mesenteric arteries and veins.
Prostaglandins (PG) were synthesized at similar rates by bovine mesenteric arteries and veins; viz., ca. 200 ng/g wet weight after one hour of incubation. After synthesis, PGE and PGF compounds were released from slices of arteries and veins into the incubating medium; PG were not detected in the walls of these blood vessels. Arachidonic acid, the precursor to PGE-2 and PGF-2-alpha, did not affect PG synthesis, whereas meclofenamate, an aspirin-like agent, decreased synthesis in arteries and veins by 90%. The PG biosynthetic capacity of these blood vessels is high, as indicated by greater than 20% conversion of (1-14C)-arachidonic acid to radiolabeled PG. Under control conditions in both arteries and veins, synthesis of PGE-2 exceeded that of PGF-2-alpha twofold. Bradykinin selectively increased the synthesis of a PGE-like substance in arteries and of a PGE-like substance in veins.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D001808:Blood Vessels / Q000187:drug effects; D001920:Bradykinin / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002417:Cattle; D002855:Chromatography, Thin Layer; D003864:Depression, Chemical; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008469:Meclofenamic Acid / Q000494:pharmacology; D008638:Mesenteric Arteries / Q000378:metabolism*; D008642:Mesenteric Veins / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis* / Q000302:isolation & purification; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D013268:Stimulation, Chemical
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D008469:Meclofenamic Acid; D001920:Bradykinin