A microplate culture method for assay of guinea pig mitogenic factor.
An improved method for assay of guinea pig mitogenic factor (MF) is described. The technique is based on the use of thymocytes in microplate cultures and yields significant savings in time, labour and materials. The quantitative potential of the assay is examined using antigen- and concanavalin A-indiced MF, and its application to assessing in vitro immune responsiveness of individual guinea pigs is explored.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002478:Cells, Cultured / Q000276:immunology*; D006168:Guinea Pigs; D007962:Leukocytes / Q000032:analysis; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000032:analysis* / Q000494:pharmacology; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis; D008934:Mitogens; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D008222:Lymphokines; D008263:Macrophage Migration-Inhibitory Factors; D008934:Mitogens