The control of immune response in vitro by Ca2+. II. The Ca2+-dependent period during mitogenic stimulation.
The Ca2+ requirement for stimulation of DNA synthesis in vitro by phytohaemagglutinin (PHA) and by lipopolysaccharide (LPS) was investigated using mouse spleen cells. During mitogenic stimulation two periods could be distinguished: a Ca2+-independent period which was followed by a Ca2+-dependent period. The results suggest that Ca2+ is required for a step preceeding DNA synthesis but not for the early initial phase of transformation.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects*; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008938:Mitosis / Q000187:drug effects*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013997:Time Factors
D002614:Chelating Agents; D003470:Culture Media; D037102:Lectins; D008070:Lipopolysaccharides; D004247:DNA; D002118:Calcium