[Semi-automated determination of urinary 17-oxosteroids in a continuous flow system (author's transl)].
A semi-automated determination of 17-oxosteroids in urinary extracts is described using a modified Zimmerman reaction in aqueous phase according to Epstein. In order to eliminate the interfering chromogens a double manifold and a double-beam colorimeter are used in continuous flow. The spectra and the chromogenecity of seven different 17-oxosteroids are presented. The specificity and the reproducibility of this technique are good and the comparison with gas-liquid chromatography and manual Zimmerman reaction shows a good correlation.
D015068:17-Ketosteroids / Q000652:urine*; D001322:Autoanalysis; D002849:Chromatography, Gas; D003124:Colorimetry; D005069:Evaluation Studies as Topic; D006801:Humans; D008722:Methods; D013997:Time Factors
D015068:17-Ketosteroids
Dosage semi-automatique en flux continu des 17-cetosteroides urinaires