[Isolation and characteristics of homopolyribonucleotide synthesized in toluene-treated E. coli cells].
Optimal conditions of homopolyribonucleotide (poly-A-14C) synthesis in toluene-treated E. coli cells under incubation with ADP-14C, Mg2+ and tris. HCl buffer (pH 8.0) are studied. Optimal Mg2+ concentration was 0.75.-10(-3) M. Heterogeneity of the isolated poly-A-14C from E. coli cell was demonstrated by means of sucrose density gradient (5-20%) centrifugation and polyacrylamide gel electrophoresis. Actinomycin D was found not to affect the reaction rate of polymerization of ADP-14C, UDP-14C and GDP-14C, catalyzed by polynucleotide phosphorylase in toluene-treated E. coli cells.
D003609:Dactinomycin / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009994:Osmolar Concentration; D011061:Poly A / Q000096:biosynthesis*; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism; D012265:Ribonucleotides / Q000378:metabolism; D014050:Toluene / Q000494:pharmacology
D012265:Ribonucleotides; D003609:Dactinomycin; D011061:Poly A; D014050:Toluene; D011117:Polyribonucleotide Nucleotidyltransferase; D008274:Magnesium
Vydelenie I kharakteristika Gomopoliribonukleotida, Sintezirobannogo Toluolobrabotannymi Kletkami E. Soli