Effect of triphenylmethane derivatives on cell-free macromolecular synthesis. I. Aminoacyl-tRNA synthetase.
The inhibitory effect of aurintricarboxylic acid (ATA) on phenylalanyl-tRNA synthetase is demonstrated in the rabbit-reticulocyte system. This inhibition is not specific to ATA; other triphenylmethane derivatives are also potent inhibitors of this enzyme reaction. The site of inhibitory action is in the enzyme itself, not in the tRNA molecule. It thus appears that the skeletal structure of ATA, not the side chains, is responsible for its inhibitory action, and that ATA is a nonspecific inhibitor of the reactions involving polynucleotides.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D002850:Chromatography, Gel; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology*; D007700:Kinetics; D010636:Phenols / Q000494:pharmacology; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*; D013329:Structure-Activity Relationship
D001565:Benzoates; D003509:Cyclohexanecarboxylic Acids; D010636:Phenols; D000604:Amino Acyl-tRNA Synthetases