Reconstitution of chromatin: mode of reassociation of chromosomal proteins.
The mode of reassociation of Ehrlich ascites histones and non-histone proteins during chromatin reconstitution was studied by sodium dodecyl sulfate polyacryl-amide gel electrophoresis. In the procedure of Bekhor et al. (I. Bekhor, G. M. Kung, and J. Bonner, (1969), J. Mol. Biol. 39, 351) most of histones and non-histone proteins reassociate with DNA in the last dialysis step of the dissociated chromatin, that is the dialysis of the chromatin in 0.4 M NaCl-5 M urea against a dilute buffer. The reassociation of histones and non-histone proteins with DNA is more gradual in the procedure of L. Kleiman and R.-C. C. Huang [(1972), J. Mol. Biol. 64, 1]. However, in both procedures the bulk of the Ehrlich ascites non-histone proteins reassociate with DNA after the binding of histones to DNA. There are small amounts of non-histone proteins which reassociate with DNA before and at the same time as histones reassociate with DNA.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002843:Chromatin / Q000032:analysis / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis; D008099:Liver / Q000032:analysis; D046911:Macromolecular Substances; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis; D009698:Nucleoproteins / Q000032:analysis; D011485:Protein Binding; D051381:Rats; D012965:Sodium Chloride; D014508:Urea
D002843:Chromatin; D006657:Histones; D046911:Macromolecular Substances; D009363:Neoplasm Proteins; D009698:Nucleoproteins; D012965:Sodium Chloride; D014508:Urea