title: A and B and A1Leb substances in glycosphingolipid fractions of human serum.
abstract: A and B and A1Leb substances were adsorbed onto red cells exposed to glycosphingolipid fractions prepared from the serum of group A and B and A1,Le(a minus b plus) donors. Group O cells exposed to fractions prepared from the serum of group A or B donors were agglutinated by an IgM cross-reacting antibody present in some group O sera. Cells exposed to fractions from A1,Le(a minus b plus) serum were agglutinated by anti-A1Leb. The amount of A substance in the fractions was related to the A subtype (A1 or A2) and to the Lewis and secretor phenotype of the donor. The uptake of blood-group substances from the lipid fractions was inhibited by the addition of whole serum to the fractions.
mesh_terms: D000017:ABO Blood-Group System / Q000032:analysis*; D002845:Chromatography; D004912:Erythrocytes / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins; D007983:Lewis Blood Group Antigens / Q000032:analysis*; D010641:Phenotype; D012824:Silicic Acid; D013107:Sphingolipids / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000017:ABO Blood-Group System; D037102:Lectins; D007983:Lewis Blood Group Antigens; D013107:Sphingolipids; D012824:Silicic Acid