Proliferation pattern of hamster melanoma cells cultured in diffusion chambers in pre-immunized hosts.
Fortner M Mel 1 melanoma cells from the Golden Hamster were capable of exponential proliferation during intraperitoneal diffusion chamber culture in xenogenic host animals (Balb/c mice, BD IX and Marshall rats). When the host animals had been preimmunized with these melanoma cells, rapid cell lysis was observed in the chambers within 4 hrs after implantation. The cell numbers were reduced to about 1 - 10% of the inoculum. At this level the cell numbers persisted for several days, as opposed to control cultures in untreated hosts, where the cells proliferated rapidly. After 6 - 10 days the cell numbers in chambers from immunized hosts slowly increased in spite of a high level of cytotoxicity in ther serum of the animals. The same pattern was seen after previous immunization with hamster lymphocytes, indicating that the antibodies were directed against a common antigen for melanoma cells and lymphocytes in hamsters. The diffusion chamber method appears to be a useful system for the study of a pure humoral immune reaction against tumour cells, and especially for investigation of the phenomenon that some tumour cells are able to escape a strong humoral cytotoxic effect from host animals.
D000818:Animals; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D003601:Cytotoxicity Tests, Immunologic; D004058:Diffusion; D005260:Female; D007114:Immunization; D008545:Melanoma / Q000276:immunology* / Q000473:pathology; D051379:Mice; D009368:Neoplasm Transplantation; D010529:Peritoneal Cavity; D051381:Rats; D012878:Skin Neoplasms / Q000276:immunology* / Q000473:pathology; D014181:Transplantation Immunology; D014183:Transplantation, Heterologous