Isolation and tissue specificity of chromatin-associated proteins in Vicia faba.
A method is described that permits extraction of one class of non-histones in 8 M urea minus 0.14 M mercaptoethanol prior to acid extraction of histones and a second class in 0.05 M Tris -1% sodium dodecyl sulfate following acid extraction of histones. Comparisons of histones and non-histones extracted by this method with those obtained by other procedures demonstrate two important advantages of the method; (1) histones obtained by this method are not contaminated by acid-soluble non-histones, and (2) non-histones are not subjected to acid or phenol during extraction. Changes in the distributions of chromatin-associated proteins in different tissues suggest that some species represent regulators of gene action.
D002843:Chromatin / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification; D008722:Methods; D009698:Nucleoproteins / Q000302:isolation & purification*; D009928:Organ Specificity; D010940:Plant Proteins / Q000302:isolation & purification*; D010944:Plants / Q000032:analysis*; D014508:Urea
D002843:Chromatin; D006657:Histones; D009698:Nucleoproteins; D010940:Plant Proteins; D014508:Urea