Heterogeneity of mitochondrial DNA from Saccharomyces cerevisiae and genetic information for tRNA.
Mitochondrial DNA from wild-type Saccharomyces cerevisiae and from an "extreme" petite mutant were analyzed by hybridization of several tRNAs on DNA fragments of different buoyant density, obtained by sonication and fractionation on a CsCl gradient. The hybridization patterns show that the genes for tRNAser, tRNAphe, tRNAhis, tRNAval, tRNAileu are present on wild-type mitochondrial DNA, while only genes for tRNAser and tRNAhis are present on petite mitochondrial DNA; moreover the hybridization patterns indicate that these genes are not clustered and suggest that more than one gene might exist for tRNAser and tRNAhis.
D002499:Centrifugation, Density Gradient; D004272:DNA, Mitochondrial / Q000032:analysis*; D007700:Kinetics; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012343:RNA, Transfer; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D004272:DNA, Mitochondrial; D012343:RNA, Transfer