title: Movement of neurosecretory product through the anatomical compartments of the neural lobe of the pituitary gland. An electron microscopic autoradiographic study.
abstract: Electron-microscope autoradiographs have been prepared from the neural lobes of the pituitary glands of rats which had received intracisternal injections of [35S] cysteine at various times before gland removal. The rate of appearance and disappearance of autoradiographically demonstrable radioactivity in the neural lobe closely paralleled that previously determined, biochemically, for radioactive hormones and neurophysins. Radioactivity was appreciably associated with the undilated parts of neurosecretory axons only during the first few hours after injection of the label. The axonal dilations were subdivided into those in which small vesicles could be seen ("endings") and those in which no small vesicles could be seen ("swellings"). Radioactivity appeared first in "endings" and then in progressively larger and larger profiles of "swellings". It appeared that newly arrived granules were found close to the limiting membrane of the nerve swelling and that as time progressed they moved deeper and deeper into the swelling. On the basis of the results, suggestions were made for an anatomical explanation of the readily-releasable pool of hormone which has been demonstrated pharmacologically.
mesh_terms: D000818:Animals; D001345:Autoradiography; D001369:Axons / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009481:Neurophysins / Q000378:metabolism; D009489:Neurosecretion; D010904:Pituitary Gland, Posterior / Q000294:innervation / Q000378:metabolism*; D010909:Pituitary Hormones, Posterior / Q000378:metabolism; D051381:Rats; D013462:Sulfur Radioisotopes
chemical_list: D009481:Neurophysins; D010909:Pituitary Hormones, Posterior; D013462:Sulfur Radioisotopes; D003545:Cysteine