Host-phage interaction on Agrobacterium tumefaciens. IV. Phage-directed protein synthesis.
Gel electrophoretic and autoradiographic techniques were used to detect the temporal sequence of protein synthesis after infection of the sensitive strain Agrobacterium tumefaciens with phage LV-1. Three classes of protein were detected: early proteins, class I, which include a protein capable of shutting off host protein synthesis; class II, proteins which are detected after 30 min; and late proteins, class III, which include the phage-directed endolysin and five additional proteins that appear 45 min after infection.
D000596:Amino Acids / Q000378:metabolism; D001345:Autoradiography; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis; D001435:Bacteriophages / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004267:DNA Viruses; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D008242:Lysogeny; D012231:Rhizobium / Q000378:metabolism*; D013997:Time Factors; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D000596:Amino Acids; D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D014764:Viral Proteins