Studies on the formation of lipid peroxides and on some enzymic activities in the liver of vitamin E-deficient rats.
Rats were fed a 5 or 20% casein diet that causes liver necrosis unless supplemented with vitamin E or selenite. The following activities were studied in liver subcellar fractions: enzymic formation of lipid peroxides, NADPH-cytochrome c reductase, oxidative demethylation of aminopyrine, and incorporation of [14C]leucine into protein (with microsomes); xanthine oxidase (with soluble supernatant); and RNA polymerases I and II (with nuclei). Formation of lipid peroxides was higher in rats fed diets without vitamin E and was not reduced significantly by dietary selenite. The activity of xanthine oxidase was higher in animals fed the 20% casein than in those fed the 5% casein diet; however, a higher activity was observed in the rats fed the latter diet without vitamin E or selenite than in those receiving these supplements. The activity of RNA polymerase I was higher in rats fed the low casein diet. Other activities examined were not affected significantly by the level of dietary casein or by vitamin E or selenits.
D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003579:Cytochrome Reductases / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004044:Dietary Proteins / Q000008:administration & dosage; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D010545:Peroxides / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D012643:Selenium / Q000494:pharmacology; D014811:Vitamin E Deficiency / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
D004044:Dietary Proteins; D010545:Peroxides; D014969:Xanthine Oxidase; D000633:Aminopyrine N-Demethylase; D003579:Cytochrome Reductases; D012321:DNA-Directed RNA Polymerases; D012643:Selenium