title: Radiosensitivities of DNA molecules in lymphocytes from the circulating blood of man.
abstract: The radiosensitivities of DNA molecules from lymphocytes of human circulating blood were examined by alkaline sucrose gradient centrifugation. The single-strand breaks of DNA per rad, including the breaks formed under the present alkaline condition, were 1.2 plus or minus 0.1 breaks per 10-12 daltons DNA per rad. When the cells were transformed, the number of breaks was found to increase to 1.8 plus or minus 0.2 breaks per 10-12 daltons DNA per rad. The lymphocytes are capable of rejoining radiation-induced single-strand breaks of DNA. The rate of rejoining was dependent upon types of the suspending medium. The rate increased to ten times of that of the non-transformed cells upon transformation.
mesh_terms: D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000097:blood / Q000528:radiation effects*; D004277:DNA, Single-Stranded; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D011830:Radiation Effects; D012710:Serum Albumin, Bovine; D013050:Spectrometry, Fluorescence; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D004277:DNA, Single-Stranded; D014316:Tritium; D012710:Serum Albumin, Bovine; D013395:Sucrose; D004247:DNA; D013936:Thymidine