The 11alpha and 17alpha-progesterone hydroxylases of Rhizopus nigricans NRRL 1477.
Differential induction of cultures of Rhizopus nigricans indicated that hydroxylation of progesterone at the 11alpha- and 17alpha-positions is due to two separate enzymes. This is supported by the finding that 11alpha- and 17alpha-hydroxylating activities are separated by differential centrigufation of cell-free extracts. The feasibility of introducing a hydroxyl group at the 11alpha- or 17alpha-position of hydroxylated progesterone derivatives was tested.
D002474:Cell-Free System; D004790:Enzyme Induction; D006900:Hydroxylation; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D011374:Progesterone / Q000032:analysis / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology* / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000302:isolation & purification / Q000378:metabolism*; D014461:Ultracentrifugation
D007527:Isoenzymes; D011374:Progesterone; D013250:Steroid Hydroxylases