A modified rosette inhibition test in the early diagnosis of renal allograft rejection.
The rosette inhibition test has been employed in the follow-up of 30 renal transplant patients using a technique which enables highly accurate marking of T-lymphocytes. The minimal inhibitory concentration (MIC) of anti-lymphocyte globulin (ALG) - that is, that concentration of ALG causing 25% inhibition of rosette formation-was higher than 1/16,000 in 32 (97%) out of 33 separate determinations made two to six days befor rejection became clinically evident, and lower than 1/16,000 in 323 (92%) out of 351 determinations after which no clinical evidence of rejection developed. The results presented in this paper indicate that this modified test is a useful tool either to predict the occurrence of or to confirm the diagnosis of rejection.
D006084:Graft Rejection; D006801:Humans; D007104:Immune Adherence Reaction / Q000379:methods*; D016030:Kidney Transplantation; D014184:Transplantation, Homologous