title: Immersion fixation of rapidly frozen, untreated tissues and suspensions of cells including spermatozoa.
abstract: Small pieces of tissue, and cell suspensions in plastic artificial insemination (AI) 'straws', were frozen rapidly in Freon-12 at -155 degrees C, without pre-treatment. Peripheral fragments were thawed directly in 2% glutaraldehyde at 0 degrees C, and processed for transmission electron microscopy (TEM) studies. Preservation of ultrastructure was satisfactory, and freezing artifacts were minimal.
mesh_terms: D000818:Animals; D003584:Cytological Techniques; D004912:Erythrocytes / Q000648:ultrastructure; D005615:Freezing; D008297:Male; D009132:Muscles / Q000166:cytology; D009206:Myocardium / Q000648:ultrastructure; D051381:Rats; D013094:Spermatozoa / Q000648:ultrastructure*; D014021:Tissue Preservation