Isolation, composition and functional properties of alpha-lactalbumins from several species.
1. Galactosyltranferases were isolated from bovine and pig milk and alpha-labtalbumins from milks of cow, pig, dog, rabbit, baboon and chimpanzee. 2. Alpha-Lactalbumins were characterized by gel electrophoresis, amino acid composition, ultraviolet absorbance, and ability to promote synthesis of lactose by the galactosyltransferases. 3. The order of K-m values of the various alpha-lactalbumins was the same with both cow and pig galactosyltransferases and differed from the order predicted from taxonomical classification of the species. 4. A280/A260 abosrbance ratios were correlated with K-m values suggesting involvement of tyrosyl groups in the function of alpha-lactalbumin.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004285:Dogs; D005260:Female; D005690:Galactose; D006026:Glycoside Hydrolases / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism; D007700:Kinetics; D007768:Lactalbumin / Q000302:isolation & purification / Q000378:metabolism; D008892:Milk / Q000201:enzymology; D002679:Pan troglodytes; D010215:Papio; D011247:Pregnancy; D011817:Rabbits; D013045:Species Specificity; D013552:Swine
D000596:Amino Acids; D007768:Lactalbumin; D006602:Hexosyltransferases; D006026:Glycoside Hydrolases; D005690:Galactose