Preparation and separation of d(pT)-10-n oligonucleotides.
A series of oligomers having the general formula d(pT)-10-n, n varying from 2 to 20, has been prepared by enzymatic joining of d(pT)-10, annealed on poly dA, employing T-4 polynucleotide ligase. The oligomers could be separated on 8 or 12% polyacrylamide gels. Such oligomers may prove useful as molecular weight markers and initiators for various polymerases.
D003090:Coliphages / Q000201:enzymology; D004267:DNA Viruses / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D008722:Methods; D008970:Molecular Weight; D009841:Oligonucleotides / Q000096:biosynthesis* / Q000302:isolation & purification; D011118:Polynucleotide Ligases; D013942:Thymine Nucleotides
D009841:Oligonucleotides; D013942:Thymine Nucleotides; D011118:Polynucleotide Ligases