Detection of lactate dehydrogenase isoenzymes in single mammalian oocytes during cleavage by a micromodivication of disc electrophoresis.
A micromodification of the method of disc-electrophoresis in glass capillary tubes is described. It can be used to study the protein composition and activity of lactate dehydrogenase isoenzymes in single rat and mouse oocytes during clevage.
D000818:Animals; D001755:Blastocyst / Q000201:enzymology; D004589:Electrophoresis, Disc / Q000379:methods*; D005260:Female; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D010063:Ovum / Q000201:enzymology*; D011247:Pregnancy; D051381:Rats
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase