Studies on glycoproteins and glycopeptides from Tn-polyagglutinable erythrocytes.
Direct evidence for the theory that Tn-polyagglutinable erythrocytes have a deficiency of alkali-labile sialic acid and galactose is gained by analysing the carbohydrate moiety of the tryptic glycopeptides with spectrophotometric methods and gas-liquid chromatography. Alkaline borohydride treatment of these glycopeptides specifically releases N-acetylgalactosaminitol. In addition it is shown that mainly the third and the first membrane glycoprotein are affected by the defect.
D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000032:analysis; D002849:Chromatography, Gas; D004589:Electrophoresis, Disc; D004912:Erythrocytes / Q000032:analysis*; D006020:Glycopeptides / Q000097:blood*; D006023:Glycoproteins / Q000097:blood*; D006601:Hexoses / Q000097:blood; D012794:Sialic Acids / Q000097:blood; D014357:Trypsin; D014462:Ultrafiltration
D006020:Glycopeptides; D006023:Glycoproteins; D006601:Hexoses; D012794:Sialic Acids; D014357:Trypsin