Removal of unwanted proteins from cell extracts by means of antiserum.
The efficiency of removal of soluble proteins in cell-free bacterial extracts by means of antiserum from rabbits immunized with similar extracts was measured. Precipitation followed by Sephadex gel-chromatography was used. Up to 80% (exceptionally 90%) removal could be obtained. The method might be applied to enrichment for "foreign" cell-extract components, for example, viral products in virus infected cells. Tests for the specificity of the method are also presented.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001407:Bacillus / Q000032:analysis; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification*; D002850:Chromatography, Gel / Q000379:methods; D003090:Coliphages; D004926:Escherichia coli / Q000032:analysis; D007106:Immune Sera / Q000302:isolation & purification; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D013045:Species Specificity
D001426:Bacterial Proteins; D007106:Immune Sera