[Transformational activity of the oncornaviruses isolated from RH and Hep-2 cell lines].
Oncornaviruses isolated from transplantable cell cultures Hep-2 and RH were used for inoculating primary tripsinized cell cultures of human foetal kidney (HFK) and human embryonic fibroblasts (HEF). In all 15 cases no transformation of HEF cells was noted. In inoculation of HFK with oncornaviruses isolated from RH cell culture in 2 of 16 cases there were obtained transformed cell cultures: HFK+VRH and HFK+VRH Mc) occurred twice as fast. The transformed cell cultures have gone through 15-23 passages and were characterized by a high mitotic activity, production of oncornaviruses, type A and B, and absence of contact inhibition, a capacity for multistratum three-dimensional growth, the modal number of chromosomes 64 and a mixed type AB of electrophoretic mobility of glucoso-6-phosphate dehydrogenase.
D002294:Carcinoma, Squamous Cell / Q000382:microbiology; D002460:Cell Line / Q000201:enzymology / Q000382:microbiology*; D002478:Cells, Cultured; D002874:Chromosome Mapping; D003588:Cytopathogenic Effect, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D007822:Laryngeal Neoplasms / Q000382:microbiology; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D009858:Oncogenic Viruses / Q000302:isolation & purification*; D013997:Time Factors; D014170:Transformation, Genetic
D005954:Glucosephosphate Dehydrogenase
O transformiruiushcheĭ aktivnosti onkornavirusov, vydelennykh iz kletochnykh linii RH i Hep-2.