Separation of creatine kinase isoenzymes in serum by ion-exchange column chromatography (Mercer's method, modified to increase sensitivity).
We describe our experience with Mercer's method [Clin. Chem. 20, 36 (1974)] for separating isoenzymes of creatine kinase (EC 2.7.3.2) in serum by ion-exchange chromatography. By using diethylaminoethyl cellulose rather than diethylaminoethyl Sephadex in the column and by changing the ionic strength of the eluting buffer, we could detect a significant proportion of the MB isoenzyme of serum creatine kinase at normal activities of creatine kinase in serum, even in the absence of cardiac disease.
D002482:Cellulose; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003402:Creatine Kinase / Q000097:blood* / Q000302:isolation & purification; D004586:Electrophoresis; D006331:Heart Diseases / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification; D008297:Male; D008722:Methods; D008832:Microchemistry; D009203:Myocardial Infarction / Q000201:enzymology
D007527:Isoenzymes; D002482:Cellulose; D003402:Creatine Kinase