Molecular properties of the inducible lupanine hydroxylase from growing cultures of Pseudomonas lupanini.
An improved method for isolation of lupanine hydroxylase, giving a 450-fold purification, is presented. The molecular weight of the enzyme is about 72 000, and the sedimentation coefficient S20, W 5.05. The enzyme contains a component similar to Pseudomonas cytochromes c. Its oxidation-reduction potential was found to be below + 45 mV.
D000470:Alkaloids / Q000494:pharmacology; D000596:Amino Acids / Q000032:analysis; D001392:Azocines / Q000494:pharmacology*; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000187:drug effects / Q000201:enzymology*; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation
D000470:Alkaloids; D000596:Amino Acids; D001392:Azocines; D006899:Mixed Function Oxygenases