Serum IgE levels during the potentiated reagin response to egg albumin in rats infected with Nippostrongylus brasiliensis.
Rats were immunized with 10 mug EA and alum; a low titer IgE anti-EA response was observed; subsequent infection with N. brasiliensis greatly potentiated this response. Serum from these rats was tested for its IgE content by a recently developed radioimmunoassay for rat IgE. The average serum level of IgE was 1.15 mug/ml; this level was not altered by immunization, but infection with N. brasiliensis was accompanied by an 80-fold increase in concentration of serum IgE. This increase could not be accounted for by IgE anti-EA or anti-worm antibodies. Infection with N. brasiliensis appears to have an adjuvant effect on IgE antibody responses to diverse environmental antigens as well as the experimentally induced IgE anti-EA response.
D000327:Adsorption; D000818:Animals; D000917:Antibody Formation; D003488:Cyanogen Bromide; D006041:Goats; D007106:Immune Sera; D007073:Immunoglobulin E / Q000032:analysis*; D007457:Iodine Radioisotopes; D008297:Male; D009194:Myeloma Proteins; D009348:Nematoda / Q000276:immunology; D009349:Nematode Infections / Q000276:immunology*; D010047:Ovalbumin; D010323:Passive Cutaneous Anaphylaxis; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats
D007106:Immune Sera; D007457:Iodine Radioisotopes; D009194:Myeloma Proteins; D007073:Immunoglobulin E; D010047:Ovalbumin; D003488:Cyanogen Bromide