Cycloheximide resistance in Chinese hamster cells. I. Spontaneous mutagenesis.
Resistance to cycloheximide (CHM) was studied in cultured Chinese hamster cells. Concentrations of CHM above 5-10-7 M were toxic for the cells. At concentrations above 9-10-7 M no colonies were recovered in selective medium. 15 resistant clones of independent origin were isolated in selective medium containing 7-10-7 M CHM. Resistance was stable when the cells were cultured under non-selective conditions. The spontaneous mutation rate was determined by the fluctuation test. Mutations to CHM resistance arose spontaneously. The spontaneous mutation rate to CHM resistance was about 10-5.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured / Q000187:drug effects*; D006224:Cricetinae; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004351:Drug Resistance; D005260:Female; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D010053:Ovary; D011336:Probability
D003470:Culture Media; D009153:Mutagens; D003513:Cycloheximide