Tryptophan biosynthetic pathway in the Enterobacteriaceae: some physical properties of the enzymes.
Several physical properties of the first four enzymatic activities of the tryptophan pathway were examined using gel filtration and ion exchange chromatography. Five different patterns were noted. Differences in the anthranilate synthetase (AS) and phosphoribosylanthranilate transferase (PRT) defined these patterns. In all the organisms studied phosphoribosylanthranilate isomerase and indoleglycerol phosphate synthetase co-eluted from both diethylaminoethyl-cellulose and G-200 and thus probably are contained in a single polypeptide of 50,000 daltons. An AS-PRT complex was found in Citrobacter species, Enterobacter cloacae, and Erwinia dissolvens. In all the other bacteria examined AS and PTR were separate molecules. In Serratia marcescens, S. marinorubra, and Enterobacter liquefaciens, AS was 140,000 daltons and PRT was 45,000 daltons. In Erwinia carotavora and Enterobacter hafniae the AS was the same size as the Serratia species but the PRT was larger at 67,000 daltons. Two Proteus species had an AS and PRT of the same size as E. carotavora and E. halfniae but the Proteus AS was different in that it partially dissociated upon gel filtration. Aeromonas formicans was unique in its possession of an AS with a molecular weight of 220,000. The PRT of A. formicans was found to elute at 67,000 daltons. Possible paths of evolution of the tryptophan enzymes are discussed in terms of the results of this study. The results presented here are also considered with respect to existing taxonomic schemes of the enteric bacteria.
D000878:Anthranilate Synthase / Q000378:metabolism; D005075:Biological Evolution; D002238:Carbohydrate Epimerases / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology*; D008970:Molecular Weight; D010430:Pentosyltransferases / Q000378:metabolism; D013045:Species Specificity; D014364:Tryptophan / Q000096:biosynthesis*; D062367:ortho-Aminobenzoates
D062367:ortho-Aminobenzoates; D014364:Tryptophan; D010430:Pentosyltransferases; D002262:Carboxy-Lyases; D000878:Anthranilate Synthase; D002238:Carbohydrate Epimerases