[Identification of antibodies by means of immunofluorescence in autoimmune diseases].
A short survey of the methods of the immunofluorescence microscopy for the demonstration of various humoral antibodies is given. It is referred to the necessity of a far-reaching standardisation, whereby especially exact data in publications are necessary. Apart from the fluorochromium/protein ratio as conjuncate criteria among others have proved the ratio of antibody protein to the total protein content and the suitability test at the antigenic material concerned. The following antibodies are dealt with in particulars; antinuclear factors, antibodies of thrombocytes, antibodies of the skeletal muscles, antibodies against smooth musculature, antibodies against mitochondria, antibodies of parietal cells, antibodies against microsomas and antibodies against structures of connective tissue.
D000906:Antibodies / Q000302:isolation & purification*; D000974:Antibodies, Antinuclear / Q000302:isolation & purification; D000917:Antibody Formation; D001327:Autoimmune Diseases / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000276:immunology; D009130:Muscle, Smooth / Q000276:immunology
D000906:Antibodies; D000974:Antibodies, Antinuclear
Nachweis von Antikörpern mit Hilfe der Immunfluoreszenz bei Autoimmunerkrankungen