Microtubule assembly and the intracellular transport of secretory granules in pancreatic islets.
Polymerized and depolymerized tubulin were measured in pancreatic islets under various physiological and pharmacological conditions. Variations in insulin release from islets of fed or fasted rats were accompanied by concomitant changes in tubulin polymerization. Glucose induced the formation of microtubules in vitro independent of extracellular calcium. Total and polymerized tubulin content were decreased by fasting and restored by glucose feeding.
D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D051381:Rats; D013806:Theophylline / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism*
D006023:Glycoproteins; D007328:Insulin; D014404:Tubulin; D013806:Theophylline; D005947:Glucose; D003078:Colchicine