title: Culture of cells in beem capsules: a new technique for electron microscopic study of monolayer cultures.
abstract: A method is described for the monolayer cultivation of primary cell suspensions and established cell lines directly in carbon-coated BEEM capsules, BEEM capsules are routinely employed by electron microscopists in tissue embedding procedures; growing monolayer cultures directly on the lids of inverted BEEM capsules presents the obvious advantage of maintaining cell to cell and cell to substratum conthaets with a minimum of stress and damage in the preparative steps for electron microscopy.
mesh_terms: D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured / Q000648:ultrastructure; D003584:Cytological Techniques; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007365:Intercellular Junctions / Q000648:ultrastructure; D010179:Pancreas / Q000166:cytology / Q000378:metabolism
chemical_list: D007328:Insulin