Translation of Semliki forest virus 42S and 26S RNAs in a cell-free system derived from Escherichia coli.
The SFV 42S RNA and the intracellular 26S RNA have been translated in a prokaryotic cell-free system, the E. coli S30. About half of the [35S]methionine-labelled products directed by both RNAs had molecular weights larger than 20,000 on polyacrylamide gels. Both products contained tryptic peptides which comigrated with all the capsid and envelope protein-derived peptides. The most striking difference between the prokaryotic and eukaryotic systems lay in the translation of the 42 S RNA: The "42S RNA-specific nonstructural" peptides, which predominate in the eukaryotic systems, were apparently absent from the product translated by the prokaryotic system.
D002474:Cell-Free System; D004926:Escherichia coli; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000032:analysis / Q000378:metabolism*; D012672:Semliki forest virus / Q000032:analysis / Q000378:metabolism*; D014357:Trypsin / Q000032:analysis; D014764:Viral Proteins / Q000096:biosynthesis*
D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins; D014357:Trypsin