Studies on the cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA.
The cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA has been studied by electron microscopy. Analysis of the electron microscopic data by means of a simple statistical mechanical model has yielded a value of 3.8-7.6 X 10(10) l./mol for the cooperative binding constant in 0.15 M NaCl. Studied under elevated salt conditions have shown that the average DNA protein complex length is 50% of the length found at 0.04 or 0.15 M NaCl.
D001426:Bacterial Proteins; D001665:Binding Sites; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004926:Escherichia coli; D008854:Microscopy, Electron; D008954:Models, Biological; D009994:Osmolar Concentration; D011485:Protein Binding
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral