Specific interaction of chromatin non-histone proteins with DNA.
The interaction of different preparations of chromatin non-histone proteins of rat liver and thymus with homologous and heterologous DNA was studied. It is shown by the method of fixation of non-histone proteins-DNA complexes on nitrocellulose filters that: (1) all the non-histone proteins preparations studied form complexes with DNA in 0.02 M Tris--HCl (pH 7.5)--3 mM MgCl2; (2) the main part of non-histone proteins interacting with DNA binds to it non-specifically; (3) a small part of non-histone proteins interacts specifically with the homologous native DNA in 5 M urea; (4) both homologous and heterologous denatured DNA binds non-histone proteins more effectively than the native one; (5) the specific interaction of non-histone proteins with the homologous denatured DNA is observed both without urea and in its presence. The specific interaction of a small part of non-histone proteins with the homologous native and denatured DNA is also shown by the method of non-histone proteins chromatography on polyacrylamide--agarose columns containing DNA. The data obtained are discussed in the light of the possible non-histone proteins role in the specific regulation of the transcription process.
D000818:Animals; D001667:Binding, Competitive; D002843:Chromatin / Q000302:isolation & purification / Q000378:metabolism*; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D009691:Nucleic Acid Denaturation; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D013950:Thymus Gland / Q000032:analysis / Q000378:metabolism*
D002843:Chromatin; D011506:Proteins; D004247:DNA