title: [Deaminase activity of Cl. botulinum type A, B, E and F cultures].
abstract: The authors present the results of a comparative study of desaminase activity in the suspensions of resting cells and in ultrasonic desintegrates of cells of Cl. botulinum types A, B, E and F against a number of amino acids and their amides. It was shown that types A, B, E and F possessed active desamination enzymes; this process, however coursed with a different degree of intensity depending on the substrate. Common for all the 4 types was the presence of desamidase L-asparaginase and L-glutaminase, and also of the desamination enzymes of the aspartic and glutamic acids. Strains of type B had the greatest set of enzymes, and of type A--the least; bacteria of types E and F occupied an intermediate position. None of the types studied contained tryptophandesaminase. Some of desaminases are bound to subcellular structures.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001215:Asparaginase / Q000378:metabolism; D002474:Cell-Free System; D003014:Clostridium botulinum / Q000201:enzymology*; D003470:Culture Media; D003641:Deamination; D005972:Glutaminase / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism
chemical_list: D000596:Amino Acids; D003470:Culture Media; D014364:Tryptophan; D001215:Asparaginase; D005972:Glutaminase
vernacular_title: Dezaminaznaia aktivnost' kul'tur Cl. botulinum tipov A, B, E i F