title: Preimplantation development in vivo and in vitro in bank voles, Clethrionomys glareolus, treated with PMSG and HCG.
abstract: Ovulation can be induced in the bank vole by PMSG and HCG and takes place 9 1/2 to 11 1/2 hr after administration of HCG. The number of eggs obtained varied from 1 to 30 (average 9.3). The highest ovulation rates were observed when PMSG and HCG were administered 41 to 43 1/2 hr apart. The embryos attained the blastocyst stage 96 hr after HCG injection. Bank vole eggs can be cultured in chemically defined media beginning with the eight-cell stage; only 25% of four-cell eggs developed to blastocysts in vitro.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D046508:Culture Techniques; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D008297:Male; D010060:Ovulation / Q000187:drug effects*; D010063:Ovum / Q000502:physiology*; D011247:Pregnancy; D012377:Rodentia / Q000196:embryology / Q000502:physiology*; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine