title: Lipogenesis and glyceride synthesis in the rat: response to diet and exercise.
abstract: The responses of hepatic and adipose tissue malic enzyme (ME), citrate cleavage enzyme (CCE), glucose-6-phosphate dehydrogenase (G6PD), and glyceride synthetase (GS) to exercise training and exhaustive exercies and the potential of a high fat or high carbohydrate diet to modify these responses were studied in male Carworth rats. Characteristic elevation and depression of ME, CCE, and G6PD were decreased in skeletal muscle, liver, and adipose tissues of high carbohydrate-fed rats. A significant two-way diet-training interaction was indicated for hepatic ME and G6PD. This interaction resulted from an apparent training modulation of ME and C6PD responses to the high fat and high carbohydrate diets. Adipose tissue G6PD was significantly decreased by training. Exhaustive exercise performed immediately prior to sacrifice did not significantly alter ME or CCE activities in either liver or adipose tissues, but decreased adipose tissue G6PD in untrained rats. Exhaustion was also associated with decreased GS activity in muscle and liver. Physical training was associated with a significant increase in GS in muscle and adipose tissues. In contrast to glyceride synthesis, no increase in adipose tissue lipogenic potential was noted in response to training, indicating that the physically trianed rat may have an enhanced ability to store but not synthesize fatty acids.
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase; D000273:Adipose Tissue / Q000201:enzymology; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004041:Dietary Fats / Q000008:administration & dosage*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis*; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000378:metabolism; D005082:Physical Exertion; D051381:Rats
chemical_list: D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005989:Glycerides; D008055:Lipids; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D001275:ATP Citrate (pro-S)-Lyase