Interaction of IgE with rat basophilic leukemia cells. III. Release of intact receptors on cell-free particles.
Rat basophilic leukemia cells which bind rat IgE-release particulate material as they lose viability. This material appears to contain the surface receptors for IgE in their native form. Up to 80% of the receptor activity has been recovered in a fraction which contains only 4 to 5% of the total cellular protein.
D000818:Animals; D001491:Basophils / Q000276:immunology*; D001666:Binding Sites, Antibody; D001798:Blood Proteins / Q000032:analysis; D002458:Cell Fractionation; D002474:Cell-Free System; D002478:Cells, Cultured; D004589:Electrophoresis, Disc; D007073:Immunoglobulin E; D007457:Iodine Radioisotopes; D007938:Leukemia / Q000276:immunology*; D051381:Rats; D012967:Sodium Dodecyl Sulfate; D013997:Time Factors
D001798:Blood Proteins; D007457:Iodine Radioisotopes; D012967:Sodium Dodecyl Sulfate; D007073:Immunoglobulin E