Computer-controlled automation of radioimmunoassay based on gel entrapment of antibody.
We describe a new, broadly applicable approach to radioimmunoassay. Solid-phase binding reagent is prepared by entrapping antibody in polyacrylamide gel. This gel is then fragmented, sieved, and dehydrated in 95 percent ethanol. Upon evaporation of the ethanol, the dry antibody-gel particles are dispensed into miniature disposable plastic columns. A unidirectional flowing stream configuration is used to construct a standard curve for the polypeptide hormone, angiotensin I, used here to exemplify the technique. Good reproducibility of this standard curve is demonstrated. The analytical system includes a computer-controlled sample turntable, digital pipet, and liquid-switching assembly.
D000178:Acrylamides; D000804:Angiotensin II / Q000276:immunology; D000906:Antibodies; D001331:Automation; D002850:Chromatography, Gel; D003201:Computers; D003202:Computers, Analog; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods*
D000178:Acrylamides; D000906:Antibodies; D000804:Angiotensin II