Use of radioimmunoassays to determine the concentration of streptococcal group-specific antibodies in rabbit antisera.
A radioimmunoassay was developed for the quantitation of antibodies to the Group A, A-variant, and C carbohydrates in rabbit streptococcal antisera. The assay employs radiolabeled purified Group carbohydrate which has been tyrosylated to allow for incorporation of 125-I. This assay has an advantage over the intitative precipitin test because it measures non-precipitating antibody in addition to precipitating antibody. Furthermore, 7S anti-IgG in rabbit antisera give a falsely elevated value for the antibody concentration in the quantitative precipitin test. This did not occur with the radioimmunoassay. The assay described is reproducible, sensitive, and uses little antisera.
D000327:Adsorption; D000645:Ammonium Sulfate; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity; D001428:Bacterial Vaccines; D011232:Chemical Precipitation; D007106:Immune Sera / Q000032:analysis*; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis; D007457:Iodine Radioisotopes; D011135:Polysaccharides, Bacterial; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D011868:Radioisotopes; D012975:Sodium Isotopes; D013291:Streptococcus / Q000276:immunology*; D014443:Tyrosine
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007106:Immune Sera; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D011135:Polysaccharides, Bacterial; D011868:Radioisotopes; D012975:Sodium Isotopes; D014443:Tyrosine; D000645:Ammonium Sulfate