Photoreactions of phosphorothioate and cysteamine-S-phosphate. Photosubstitution and photophosphoryl transfer.
The photoreactions of phosphorothioate and cysteamine-S-phosphate were investigated. On irradiation of phosphorothioate a marked change in absorption spectrum was observed. The product migrated in high voltage electrophoresis, with different mobility from that of phosphorothioate and its dimer, or inorganic orthophosphate. It contained phosphate and sulfur in a ratio of 2: 1, without reducing properties. Therefore it was suggested that the product is either pyrothiophosphate, or a cyclic compound, with similar composition. On irradiation of phosphorothioate in the presence of potential phosphoryl group acceptor, such as glucose or galactose, 25-40% of the phosphoryl group was transferred. The formation of glucose 6-phosphate, or galactose 6-phosphate was observed. The photolysis of cysteamine-S-phosphate gave cysteamine, inorganic orthophospate and taurine. Under the same conditions of irradiation, inorganic orthophosphate or aminoethanol-O-phosphate were found to be stable.
D003543:Cysteamine / Q000031:analogs & derivatives / Q000528:radiation effects; D004590:Electrophoresis, Paper; D005690:Galactose; D005947:Glucose; D006600:Hexosephosphates; D007700:Kinetics; D009943:Organophosphorus Compounds / Q000528:radiation effects; D009946:Organothiophosphorus Compounds / Q000528:radiation effects; D010782:Photolysis; D010785:Photophosphorylation; D011830:Radiation Effects; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
D006600:Hexosephosphates; D009943:Organophosphorus Compounds; D009946:Organothiophosphorus Compounds; D003543:Cysteamine; D005947:Glucose; D005690:Galactose