Analysis of the diazinon metabolites G 27550 and GS 31144 by gas-liquid chromatography with nitrogen-specific detection after derivatization.
The alkylation and silylation of the diazinon metabolites G 27550 (2-isopropyl-4-methyl-6-hydroxypyrimidine) and GS 31144 [2-(1'-hydroxy-1'-methyl)-ethyl-4-methyl-6-hydroxypyrimidine] with methyl iodide/sodium hydride and bis-(trimethylsilyl)-trifluoroacetamide, respectively, were examined for use as confirmation techniques for these metabolites in sample material. The methods proved to be rapid and simple. The derivatives chromatographed well on 4% SE-30/6% QF-1. Electrolytic conductivity detection proved satisfactory for the derivatives with minimum cleanup. The techniques were successfully applied to the analysis of the two metabolites in dog urine in the range of 1 ppm.
D000478:Alkylation; D000818:Animals; D002250:Carbon Radioisotopes; D002849:Chromatography, Gas; D004285:Dogs; D004553:Electric Conductivity; D006900:Hydroxylation; D013058:Mass Spectrometry; D008745:Methylation; D009584:Nitrogen; D009946:Organothiophosphorus Compounds; D011743:Pyrimidines / Q000652:urine*; D013696:Temperature
D002250:Carbon Radioisotopes; D009946:Organothiophosphorus Compounds; D011743:Pyrimidines; D009584:Nitrogen