panD, a new chromosomal locus of Salmonella typhimurium for the biosynthesis of beta-alanine.
Three mutants of Salmonella typhimurium LT2, which required either pantothenate or beta-alanine for growth, were obtained after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Their phenotype was: SM30 Pan-, SM31 Pan- Met-, SM32 Pan- Thi- (requirement for the thiazole-moiety of thiamine). Neither aspartate, dihydrouracil, nor beta-ureidopropionate replaced beta-alanine as growth factor. By conjugation it was found that the three genetic lesions (Pan-, Met-, Thi-) were located at about minute 128 of the bacterial chromosome. By transduction 63% linkage was found between the Pan and Met loci, and 84% between the Thi and Pan loci. Probably the thiazole auxotrophy was due to a lesion in the thiG locus. The new genetic locus responsible for the synthesis of beta-alanine was named panD.
D000409:Alanine / Q000096:biosynthesis*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D008040:Genetic Linkage; D009154:Mutation; D010205:Pantothenic Acid / Q000378:metabolism; D010641:Phenotype; D012486:Salmonella typhimurium / Q000378:metabolism*
D010205:Pantothenic Acid; D000409:Alanine