Augmentation of delayed-type hypersensitivity by doses of cyclophosphamide which do not affect antibody responses.
Mice immunized with more SRBC than are required to produce optimal delayed-type hypersensitivity reactions, developed good antibody responses and poor delayed foot pad reactions. Cyclophosphamide treatment in low doses (20 mg/kg) before immunization, augmented the delayed-type hypersensitivity without affecting antibody responses. Cyclophosphamide did not augment delayed responses to optimal doses of SRBC (0.01%), but did augment the delayed hypersensitivity response of mice immunized with a suboptimal antigen dose (0.001%); which produced no detectable antibody response with or without cyclophosphamide pretreatment. These results suggest that antibody feedback is not the sole regulator of delayed reactions; the possibility that suppressor T cells may also be involved is discussed.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D003520:Cyclophosphamide / Q000008:administration & dosage* / Q000276:immunology / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D005246:Feedback; D005777:Geese / Q000276:immunology; D006614:Hindlimb; D006736:Horses / Q000276:immunology; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008814:Mice, Inbred NZB; D012756:Sheep / Q000276:immunology
D003520:Cyclophosphamide