Radioimmunoassay of serum pancreatic amylase in normal and pancreatectomized pigs (38504).
A double antibody radioimmunoassay has been developed that is capable of assaying and discriminating small amounts of pancreatic amylase even in the presence of amylase isoenzymes. Assays on adult pigs indicate a mean serum concentration of pancreatic amylase of 2.4mug/ml. To date, no other technique can offer such a discrimination of the pancreatic amylase in the circulation. Following pancreatectomy, the amount of radioimmunoassayable amylase in the circulation disappeared within 48 hr. This was reflected by a simultaneous decline in the serum amylase enzyme activity. Within 48 hr after pancreatectomy, the serum amylase enzyme activity had declined 60%. Thus, in pigs, the pancreas contributes significantly to the circulating amylase levels.
D000681:Amylases / Q000097:blood*; D000818:Animals; D003429:Cross Reactions; D007106:Immune Sera; D007527:Isoenzymes; D010179:Pancreas / Q000201:enzymology*; D010180:Pancreatectomy; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013552:Swine
D007106:Immune Sera; D007527:Isoenzymes; D000681:Amylases