Effect of the exotoxin of Bacillus thuringiensis on the biosynthesis and maturation of mouse liver nuclear RNA.
The effect of the exotoxin of Bacillus thuringiensis on the in vivo incorporation of [14-C] orotic acid into mouse liver nuclear rRNA and low molecular weight RNA was studied. The following results were obtained. 1. The exotoxin does not inhibit the synthesis of 45 S pre-rRNA, but causes a breakdown of these molecules. 2. The exotoxin inhibits the conversion of 38 S pre-rRNA into 32 S and 21 S. 3. The exotoxin inhibits the labelling of nuclear 5 S RNA, whereas the labelling of 4.6 S pre-tRNA is not affected. It is suggested that 5 S RNA may control the processing of 45 S pre-rRNA.
D000362:Agar; D000818:Animals; D001407:Bacillus; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009963:Orotic Acid / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D014118:Toxins, Biological / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D012335:RNA, Ribosomal; D014118:Toxins, Biological; D009963:Orotic Acid; D012313:RNA; D000362:Agar