title: Glycopeptides from the egg jelly coat of the toad Bufo vulgaris.
abstract: 1. Glycopeptides isolated by exhaustive pronase digestion from the egg jelly coat of toad, Bufo vulgaris, were separated by DEAE-cellulose chromatography into two fractions. These fractions each gave a single band when they were subjected to electrophoresis on cellulose acetate strips. The glycopeptides behaved as a kind of "macroglycopeptide" on gel chromatography. 2. The glycopeptide fractions contained as sugar components galactosamine, glucosamine, galactose, fucose and N-acetylneuraminic acid. The amino acid composition was characterized by the presence of threonine and serine, which accounted for approximately 70% of the total amino acids. 3. Large amounts of galactosamine, threonine and serine were destroyed by treatment with alkali, indicating that the carbohydrate-peptide linkage in the glycopeptides is mostly an O-glycosidic bond between N-acetylgalactosamine and the hydroxy groups of threonine and serine.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002025:Bufonidae; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D004586:Electrophoresis; D005260:Female; D006020:Glycopeptides / Q000302:isolation & purification; D006601:Hexoses / Q000032:analysis; D010063:Ovum / Q000032:analysis*; D011485:Protein Binding; D012794:Sialic Acids / Q000032:analysis
chemical_list: D000596:Amino Acids; D006020:Glycopeptides; D006601:Hexoses; D012794:Sialic Acids