A rapid, quantitative determination of clottable fibrinogen unaffected by heparin.
A turbidimetric assay for clottable plasma fibrinogen which is not sensitive to heparin or Pyran inhibition is described. The basis of the assay is the substitution of Reptilase-R for the thrombin usually employed. The assay correlates very well with a thrombin turbidimetric method and also has other advantages, including better stability of the clotting enzyme and more rapid attainment of equilibrium.
D005340:Fibrinogen / Q000032:analysis*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D009391:Nephelometry and Turbidimetry; D010447:Peptide Hydrolases / Q000378:metabolism; D010783:Photometry; D011714:Pyrans / Q000494:pharmacology; D012910:Snake Venoms; D013053:Spectrophotometry; D013917:Thrombin / Q000032:analysis / Q000378:metabolism
D011714:Pyrans; D012910:Snake Venoms; D005340:Fibrinogen; D006493:Heparin; D010447:Peptide Hydrolases; D013917:Thrombin