Mutations in Escherichia coli that relieve catabolite repression of tryptophanase synthesis. Tryptophanase promoter-like mutations.
From a strain lacking adenyl cyclase and the catabolite-sensitive gene activator protein, two mutants were isolated that can synthesize tryptophanase. Each mutation is extremely closely linked to the tryptophanase structural gene. The mutations differ from one another in the rate of synthesis of tryptophanase that they permit in the genetic background in which they were isolated; they differ from one another and also from the wild type in the maximum rate of synthesis of tryptophanase that they permit in a genetic background with intact adenyl cyclase and catabolite-sensitive gene activator protein. Both mutations appear to lie in the tryptophanase promoter.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003090:Coliphages; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology*; D005796:Genes; D008040:Genetic Linkage; D008190:Lyases / Q000096:biosynthesis*; D009154:Mutation; D009876:Operon; D011995:Recombination, Genetic; D014161:Transduction, Genetic; D014368:Tryptophanase / Q000096:biosynthesis*
D003470:Culture Media; D008190:Lyases; D014368:Tryptophanase