Membrane-bound S-100 protein on nerve cells and its distribution.
In this study we present data supporting a heterogeneous distribution of the brain-specific S-100 protein on nerve cell plasma membranes from isolated neurons. This has been shown in a previous study by immunoelectron microscopy using peroxidase-conjugated anti-S-100 antiserum and in this study by immunofluorescence using fluorescein-conjugated anti-S-100 antiserum, the specificity of which is discussed. Incubation of the cells at +23 degrees C or at +4 degrees C did not change the distribution pattern of S-100 over the cell membranes, nor did incubation in antimetabolites, or incubation with univalent Fab fragments. We conclude that the membrane-bound S-100 protein with its heterogeneous and polar distribution on the nerve cells is a sign of a protein differentiation of the neuron.
D000818:Animals; D001386:Azides / Q000494:pharmacology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D005455:Fluorescent Antibody Technique; D007122:Immunoelectrophoresis; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D009418:S100 Proteins / Q000276:immunology / Q000378:metabolism*; D013696:Temperature
D001386:Azides; D009419:Nerve Tissue Proteins; D009418:S100 Proteins; D003208:Concanavalin A; D011691:Puromycin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003513:Cycloheximide