Purification and concentration of influenza inactivated viruses by continuous-flow zonal centrifugation.
A mathod is described for the purification, on an industrial scale, of influenza viruses grown in allantoic cavity of embryonated eggs.  The mehtod consists of combining continuous-flow centrifugation with zonal centrifugation in a sucrose (36.6 per cent-52.5 per cent w/v) density gradient.  The sample flow rate is approximately 3.7 litres/h and the volumes treated vary between 3 and 33 litres of allantoic fluid.  Both the recovery of the virus and the degree of concentration and purification result satisfactory.
D000482:Allantois; D000818:Animals; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D002642:Chick Embryo; D007252:Influenza Vaccines; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D014776:Virus Cultivation
D007252:Influenza Vaccines