The raising and characterization of cortisol-binding antiserum.
Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail. The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such. The specificity of the harvested antiserum and transcortin towards the coupling of several related steroids are compared; the rabbit antiserum appears to be the more selective binding agent.
D000818:Animals; D000917:Antibody Formation; D001666:Binding Sites, Antibody; D002850:Chromatography, Gel; D006854:Hydrocortisone / Q000276:immunology*; D007106:Immune Sera; D007457:Iodine Radioisotopes; D008722:Methods; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D014156:Transcortin
D007106:Immune Sera; D007457:Iodine Radioisotopes; D014156:Transcortin; D006854:Hydrocortisone