title: Characterization of the lipids of mesosomal vesicles and plasma membranes from Staphylococcus aureus.
abstract: Mesosomal vesicles and plasma membranes were isolated from Staphylococcus aureus ATCC 6538P by protoplasting and differential centrifugation. The lipids of each of the two membrane fractions were extracted with pyridine-acetic acid-N-butanol, and the nonlipid contaminants were removed by Sephadex treatment. The lipids were then separated by passage through diethylaminoethyl-cellulose columns and characterized by thin-layer chromatographic, chemical, and spectral analyses. The lipids were separated into four discrete diethylaminoethyl fractions: (i) vitamin K2, carotenoids, C55 isoprenoid alcohol, and monoglucosyl diglyceride; (ii) cardiolipin, carotenoids, phosphatidyl glycerol, diglucosyl diglyceride, and an unidentified ninhydrin-positive component; (iii) cardiolipid and phosphatidyl glyderol; (iv) cardiolipin, phosphatidyl glycerol, and phosphatidyl glucose. Qualitatively, no difference in lipid composition between mesosomal vesicles and plasma membranes was found. However, based on equal dry weights of membrane materials, a relative quantitative difference in the amount of specific lipids in mesosomal vesicles and plasma membranes was observed. There are 4 times more monoglucosyl diglyceride, 2.6 times more diglucosyl diglyceride, 3.8 times more phosphatidyl glucose, 2 times more carotenoids, and 2 times more vitamin K2 found in mesosomal vesicles than in plasma membranes. The concentration of cardiolipin and phosphatidyl glycerol is 3.6 and 6 times greater, respectively, in mesosomal vesicles.
mesh_terms: D002308:Cardiolipins / Q000032:analysis; D002338:Carotenoids / Q000032:analysis; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D002855:Chromatography, Thin Layer; D004075:Diglycerides / Q000032:analysis; D005233:Fatty Alcohols / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D011523:Protoplasts; D012997:Solvents; D013210:Staphylococcus / Q000032:analysis / Q000648:ultrastructure*; D014812:Vitamin K / Q000032:analysis
chemical_list: D002308:Cardiolipins; D004075:Diglycerides; D005233:Fatty Alcohols; D006027:Glycosides; D008055:Lipids; D010743:Phospholipids; D012997:Solvents; D014812:Vitamin K; D002338:Carotenoids