Rapid method for preparing cell-free extracts of Aspergillus ochraceus.
A rapid method for preparing cell-free extracts of Aspergillus ochraceus was developed.  Mycelial mats were prefrozen in liquid nitrogen, ground to a fine powder in a cold mortar, and homogenized in an all-glass mechanical homogenizer.  This method provided preparations averaging 43.0 mg of protein per g of mycelium (wet weight).  The method was fast, efficient, and did not subject the extract to temperatures above 1 C or to heavy metals.  The preparation method was suitable for studying a variety of in vitro fungal enzyme systems.  Amylase, acid phosphate dehydrogenase, beta-glucosidase, beta fructofuranosidase, and trehalase activities were measurable in the preparations.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000681:Amylases / Q000378:metabolism; D001230:Aspergillus / Q000201:enzymology; D002374:Catalase / Q000378:metabolism; D002458:Cell Fractionation / Q000379:methods*; D002474:Cell-Free System; D064429:Fatty Acid Synthases / Q000378:metabolism; D005615:Freezing; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D011312:Pressure; D013393:Sucrase / Q000378:metabolism; D014198:Trehalase / Q000378:metabolism
D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D064429:Fatty Acid Synthases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000681:Amylases; D005959:Glucosidases; D014198:Trehalase; D013393:Sucrase