[Purification and some properties of the thymidine kinase from the rat thymus].
Preparation of thymidine kinase, purified 500-fold, was isolated from rat thymus by means of fractionation with ammonium sulphate, gel filtration on Sephadex G-200, treatment with calcium phosphate gel and chromatography on DEAE-cellulose. The enzyme was shown to be stabilized by 0.12 mM of thymidine, activated by Mg2 (optimum concentration 12 mM) and inhibited by Mn2+ATP served as donor of phosphate groups in reactions, catalyzed by thymidine kinase. In respect to the phosphate acceptor the enzyme showed sharp specificity: it used as a substrate only thymidine, deoxyuridine and its derivatives, substituted at the 5-th position by haloid group. In study of affinity of the enzyme for the substrate Km equals 2.5-10 minus 5 M (Vmax equals 0.09) was determined.
D000818:Animals; D002250:Carbon Radioisotopes; D002845:Chromatography / Q000379:methods; D002854:Chromatography, Paper; D003720:Densitometry; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D004794:Enzyme Repression; D051381:Rats; D012588:Scintillation Counting; D013936:Thymidine / Q000302:isolation & purification; D013937:Thymidine Kinase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D013950:Thymus Gland / Q000201:enzymology*; D014461:Ultracentrifugation
D002250:Carbon Radioisotopes; D013937:Thymidine Kinase; D013936:Thymidine
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