Nothing dehydrogenase reaction as an artefact in serum isoenzyme analyses.
A nonspecific staining occurs in serum in isoenzyme methods based on tetrazolium staining reactions. The artefact is pronounced when a large application volume, a prolonged incubation time, distinctly alkaline conditions, or excess of phenazine methosulphate is used. Only phenazine methosulphate and a tetrazolium stain are required for the appearance. Inhibition studies and histochemical staining reactions give evidence that protein-bound sulphydryl groups are responsible for the artefact in serum.
D002729:Chloromercuribenzoates / Q000494:pharmacology; D003402:Creatine Kinase / Q000097:blood*; D003486:Cyanides / Q000494:pharmacology; D005978:Glutathione; D006801:Humans; D007202:Indicators and Reagents; D007527:Isoenzymes / Q000097:blood*; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008722:Methods; D008773:Methylphenazonium Methosulfate; D009203:Myocardial Infarction / Q000201:enzymology; D009284:Naphthols; D010088:Oxidoreductases / Q000097:blood*; D013438:Sulfhydryl Compounds / Q000097:blood; D013778:Tetrazolium Salts
D002729:Chloromercuribenzoates; D003486:Cyanides; D007202:Indicators and Reagents; D007527:Isoenzymes; D009284:Naphthols; D013438:Sulfhydryl Compounds; D013778:Tetrazolium Salts; D008773:Methylphenazonium Methosulfate; D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D005978:Glutathione