Properties of Ascaris muscle mitochondria. 1. Cytochromes.
1. The cytochrome system in Ascaris muscle mitochondria was further characterized using purer preparations. 2. Difference spectra (at 22 degrees C and -196 degrees C) of the mitochondrial preparations using succinate and ascorbate plus N,N,N' ,N'-tetramethyl-p-phenylenediamine show that Ascaris muscle mitochondria contain cytochromes c1, c and aa3, and also at least three b-type cytochromes. The b-type cytochrome is the predominant component. 3. Cytochrome c and Ascaris cytochrome b-560 can be extracted from the mitochondrial preparations with 150mM KCl, leaving the membrane-bound cytochromes c1, b and aa3 in the KCl residue.
D000818:Animals; D001200:Ascaris / Q000201:enzymology*; D001205:Ascorbic Acid; D001665:Binding Sites; D002248:Carbon Monoxide; D003580:Cytochromes / Q000302:isolation & purification / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000201:enzymology*; D010084:Oxidation-Reduction; D010655:Phenylenediamines; D011189:Potassium Chloride; D011485:Protein Binding; D011487:Protein Conformation; D013053:Spectrophotometry; D013386:Succinates; D013696:Temperature
D003580:Cytochromes; D010655:Phenylenediamines; D013386:Succinates; D011189:Potassium Chloride; D002248:Carbon Monoxide; D001205:Ascorbic Acid