Placental acid hydrolase purification on concanavalin A-sepharose.
Crude human placental extract was chromatographed on concanavalin A-Sepharose. Using a single procedure, 20- to over 300-fold purification of six acid glycohydrolases was achieved, in most cases with a good yield. Improved yield of individual enzymes could be achieved by modifications of the method of elution. The capacity of concanavalin A-Sepharose to bind glycohydrolases from placenta was very large, and will provice a useful procedure for the large-scale purification of the human enzymes.
D002845:Chromatography; D003208:Concanavalin A; D005260:Female; D005696:Galactosidases / Q000302:isolation & purification; D005959:Glucosidases / Q000302:isolation & purification; D005966:Glucuronidase / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism; D006596:Hexosaminidases / Q000302:isolation & purification; D006801:Humans; D006867:Hydrolases / Q000302:isolation & purification*; D008358:Mannose; D008759:Methylglycosides; D010920:Placenta / Q000201:enzymology*; D011134:Polysaccharides; D011247:Pregnancy; D011506:Proteins / Q000302:isolation & purification; D012685:Sepharose; D014020:Tissue Extracts
D008759:Methylglycosides; D011134:Polysaccharides; D011506:Proteins; D014020:Tissue Extracts; D003208:Concanavalin A; D012685:Sepharose; D006867:Hydrolases; D005696:Galactosidases; D005959:Glucosidases; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; D005966:Glucuronidase; D008358:Mannose