[Comparative characteristics of 2 methods of separating mouse T- and B-lymphocytes].
The results of separation of T- and B-cells of the C3H/Sn and AKR/j micr lymph nodes with AKR-anti-theta3H and C3H-thetaAKR isosera and cellular immunosorbents containing the antigen-antibody complexes were analyzed in this work. The strain differences in the theta+-cells (T-lymphocytes) level were demonstrated in the untreated mice of the noted strains. Populations enriched by B-lymphocytes under different methods of separation differed somewhat in composition. The cellular immunosorption had many advantages in comparison with the cytotoxic-immune sera used for the T- and B-cell separation.
D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D003601:Cytotoxicity Tests, Immunologic; D007106:Immune Sera; D007158:Immunologic Techniques; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D000936:Antigen-Antibody Complex; D000941:Antigens; D007106:Immune Sera
Sravnitel'naia kharakteristika dvukh metodov razdeleniia T- i B-limfotsitov mysheĭ