[Application of the automatic Edman procedure to establishing the primary structure of swine pepsinogen and its fragments].
The automatic Edman procedure was applied to elucidate N-terminal sequences of swine pepsinogen, pepsin, and the fragments of its degradation by BrCN, i. e. B-1 and B-5. A "Beckman"  model 890 instrument was used in experiments. 50 amino acid residues were split off the pepsinogen molecule and identified and 55 amino acid residues-off the pepsin molecule by means of gas chromatography. A continuous N-terminal sequence of pepsinogen was 119 amino acids, in which the overlapping of the known peptide sequences with enzymic hydrolysers was taken into account. In B-1, B-4, B-5 fragments 22, 31 and 38, residues, respectively were analyzed with the sequencer.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001322:Autoanalysis; D010434:Pepsin A / Q000032:analysis*; D010435:Pepsinogens / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D013552:Swine; D013861:Thiocyanates
D000596:Amino Acids; D010435:Pepsinogens; D010446:Peptide Fragments; D013861:Thiocyanates; D010434:Pepsin A
Primenenie avtomaticheskogo metoda Edmana dlia ustanovleniia pervichnoĭ struktury svinogo pepsinogena, pepsina i ego fragmentov