A nonchromatographic radioimmunoassay for 17alpha-hydroxyprogesterone.
A radioimmunoassay is described for the measurement of 17alpha-hydroxyprogesterone in human plasma. We have employed the principle of "immunologic purification." 17-OHP and related steroids are bound to an excess of antiserum. Steroids with a low affinity for the antibody are extracted by ether. The 17-OHP is subsequently freed from antibody by acid hydrolysis and this extract is assayed by radioimmunoassay.
D000328:Adult; D000937:Antigen-Antibody Reactions; D002648:Child; D002850:Chromatography, Gel; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood*; D007106:Immune Sera; D008297:Male; D008598:Menstruation; D008722:Methods; D010049:Ovarian Diseases / Q000097:blood; D011863:Radioimmunoassay; D013997:Time Factors; D014316:Tritium
D006908:Hydroxyprogesterones; D007106:Immune Sera; D014316:Tritium