Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the breakdown of cellulose. 2. Activities of the five endo-1,4-beta-glucanases towards carboxymethylcellulose.
A complementary discussion is given of an earlier presented theory for viscometric determination of enzymic activity towards polymer substrates. Five different, purified endo-1,4-beta-glucanases from the fungus Sporotrichum pulverulentum have been characterised with respect to their molecular activities(Am) and Michaelis-Menten constants (Km). The errors in the Am and Km values are discussed. The Am value seems to be more valuable as a distinguishing parameter than the Km value.
D002266:Carboxymethylcellulose Sodium / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D008747:Methylcellulose / Q000031:analogs & derivatives*; D013175:Sporothrix / Q000201:enzymology*; D014783:Viscosity
D008747:Methylcellulose; D006026:Glycoside Hydrolases; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium