title: [Detoxification of a purified concentrated C1. oedematiens toxin].
abstract: One of the stages of a new technological scheme for obtaining C1. oedematiens toxoid (detoxication with formaldehyde of a purified concentrated toxin) was elaborated under experimental conditions. It was found that under the effect of increased formalin concentrations (0.4--0.2%) or of prolonged action of heat there developed in the toxin a reduction of antigenic activity, expressed on immunoelectrophoregrams in the form of a change of surface potential of one of the electrophoretic fractions. Detoxication of the purified concentrated C1. oedematients toxin occurred in the course of 3 days without any losses of its antigenic activity under the following conditions: formalin concentration 0.1%, temperature 37-38 degrees C, pH L.7--6.9. No reversion of toxicity of the preparations detoxified under the mentioned conditions was revealed under the action of formalin-binding agent (sodium sulfite) on them.
mesh_terms: D003013:Clostridium; D005557:Formaldehyde; D006358:Hot Temperature; D007122:Immunoelectrophoresis; D014118:Toxins, Biological / Q000302:isolation & purification; D014121:Toxoids / Q000302:isolation & purification
chemical_list: D014118:Toxins, Biological; D014121:Toxoids; D005557:Formaldehyde
vernacular_title: Obezvrezhivanie ochishchennogo kontsentrirovannogo toksina C1. oedematiens