Enzyme-linked immunoassay. I. Novel method for synthesis of the insulin-beta-D-galactosidase conjugate and its applicability for insulin assay.
Pork insulin was subjected to mercaptosuccinylation and then coupled to beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N,N'-o-phenylenedimaleimide. The competitive binding of the conjugate and insulin to anti-insulin antibody was tested. Results showed that formation of an insulin-beta-D-galactosidase conjugate could be used for immunoassay of insulin.
D001667:Binding, Competitive; D004926:Escherichia coli / Q000378:metabolism; D005696:Galactosidases / Q000378:metabolism*; D007118:Immunoassay / Q000379:methods*; D007328:Insulin / Q000032:analysis*; D008301:Maleimides; D013438:Sulfhydryl Compounds
D007328:Insulin; D008301:Maleimides; D013438:Sulfhydryl Compounds; D005696:Galactosidases