Lipase-triggered kinetic assay of serum triglycerides.
We describe a kinetic method for assay of serum or plasma triglycerides, by use of an enzymatic hydrolysis and reaction sequence already described [Clin. Chem. 19, 476 (1973)]. The reaction is triggered by addition of lipase, at a time when free glycerol, or pyruvate (or both) are no longer present. In this method, therefore, there is no need for a blank glycerol assay. In the procedure, reagents are used that are available commercially in the form of stable, dry powders; the method for the preparation of the reagents has been changed to achieve improved stability and performance. Stability and recovery of added triglycerides are satisfactory.
D001322:Autoanalysis; D004355:Drug Stability; D005069:Evaluation Studies as Topic; D006801:Humans; D007700:Kinetics; D008049:Lipase / Q000378:metabolism; D008722:Methods; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014280:Triglycerides / Q000097:blood*
D014280:Triglycerides; D008049:Lipase