Effects of insulin and of NSILA-S on the perfused rat heart: glucose uptake, lactate production and efflux of 3-0-methyl glucose.
200 muU of insulin per ml perfusion medium stimulated glucose uptake and lactate production by the isolated perfused rat heart about 5 and 10 fold, respectively. Nonsuppressible insulin-like activity (NSILA-S) extracted from human serum had almost the same maximal effects. Mol per mol the two substances were equally effective and, correspondingly, the dose-response curves were very similar. NSILA-S accelerated the efflux of 3-0-methyl glucose from the heart cells to the same extent as insulin. It can be concluded that NSILA-S acts on heart muscle in a very similar way to that of insulin.
D000818:Animals; D001692:Biological Transport; D005947:Glucose / Q000378:metabolism*; D006321:Heart / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007773:Lactates / Q000096:biosynthesis*; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D010477:Perfusion; D051381:Rats
D007328:Insulin; D007773:Lactates; D010455:Peptides; D005947:Glucose