title: Modified nucleosides and bizarre 5'-termini in mouse myeloma mRNA.
abstract: Messenger RNAs from mouse myeloma cells contain N-6-methyl adenosine and novel 5' termini having 7-methy-guanosine in a 5', 5' triphosphate linkage with ribose-methylated nucleosides. Ten common 5'-terminal sequences of the forms m-7-G-5'-PPP-5'-NmpNp and m-7-G-5'-ppp-5'-NmpNmpNp are reported. Structures like this may be a general feature of mRNA in eukaryotes.
mesh_terms: D000227:Adenine Nucleotides / Q000032:analysis; D000241:Adenosine / Q000032:analysis; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D002460:Cell Line; D004586:Electrophoresis; D006151:Guanosine / Q000032:analysis; D051379:Mice; D009101:Multiple Myeloma / Q000032:analysis*; D009705:Nucleosides / Q000032:analysis*; D011119:Polynucleotides / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis
chemical_list: D000227:Adenine Nucleotides; D009705:Nucleosides; D011119:Polynucleotides; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D006151:Guanosine; D012343:RNA, Transfer; D000241:Adenosine