Determination of phosphate in serum and urine by a single step malachite-green method.
The highly sensitive malachite-green method for the determination of phosphate requires a protective colloid in order to avoid precipitation of the formed dye salt. A polyvinylalcohol proved to be suitable and permit the determination of phosphate in urine and in serum without prior precipitation of the proteins. The present method is suitable for a one-step procedure which allows the analysis of several hundred samples per man-hour and is applicable to microquantities of serum or urine.
D001597:Benzylidene Compounds; D004396:Coloring Agents; D003956:Dialysis; D008722:Methods; D010710:Phosphates / Q000032:analysis* / Q000097:blood / Q000652:urine; D011142:Polyvinyl Alcohol; D000644:Quaternary Ammonium Compounds; D013053:Spectrophotometry
D001597:Benzylidene Compounds; D004396:Coloring Agents; D010710:Phosphates; D000644:Quaternary Ammonium Compounds; D011142:Polyvinyl Alcohol