Enrichment of photosystem I reaction center chlorophyll from spinach chloroplasts.
The reaction center chlorophyll of Photosystem I in spinach chloroplasts was highly enriched. Preparations having 5-9 chlorophylls per 1 P700 were obtained by treating the Photosystem I particles prepared by digitonin treatment of chloroplasts with wet diethyl ether. All P700 present in the extracted particles was found to be photoactive, undergoind oxidation upon illumination.
D001665:Binding Sites; D002734:Chlorophyll / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D004227:Dithionite; D007700:Kinetics; D008027:Light; D010084:Oxidation-Reduction; D010785:Photophosphorylation; D010944:Plants; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D002734:Chlorophyll; D004227:Dithionite