A method for the in vitro measurement of tensions of blood gases with a mass spectrometer.
A specially designed cuvette with an in vivo catheter allows the in vitro measurement of gas tensions in heparinized samples of blood with a mass spectrometer. Approximately 1.5 cc of blood is required, half of which is used to clear the dead space of the cuvette. The partial pressures of oxygen (P-o2) and of carbon dioxide (Pco2) of arterial and venous samples obtained from the catheterized femoral arteries and veins of 10 mongrel dogs were measured with the mass spectometer and with a conventional pH/gass analyzer. Good agreement was obtained between the two methods. The range of validation for P-o2 was between 35 and 100 mmHg; for P-co2 it was between 20 and 88 mmHg. Depletion, temperature control, flow dependency, and calibration problems are discussed.
D000818:Animals; D001784:Blood Gas Analysis / Q000295:instrumentation; D002245:Carbon Dioxide / Q000097:blood*; D002404:Catheterization / Q000295:instrumentation*; D004285:Dogs; D013058:Mass Spectrometry / Q000295:instrumentation*; D010100:Oxygen / Q000097:blood*; D010313:Partial Pressure
D002245:Carbon Dioxide; D010100:Oxygen