title: Derepression and repression of the histidine operon: role of the feedback site of the first enzyme.
abstract: Thiazolealanine, a false feedback inhibitor, causes transient repression of the his operon previously derepressed by a severe histidine limitation in strains with a wild-type or feedback-hypersensitive first enzyme but not in feedback-resistant mutants. Since experiments reported here clearly demonstrate that thiazolealanine is not transferred to tRNAHis, it is proposed that this "transient repression" is effected through the interaction of thiazolealanine with the feedback site of the enzyme. Experiments in the presence of rifampin indicate that this thiazolealanine-mediated effect is exerted at the level of translation. We conclude that histidine (free), in addition to forming co-repressor, also represses the operon at the level of translation through feedback interaction with the first enzyme of the pathway (adenosine 5'-triphosphate phosphoribosyltransferase). Rates of derepression in feedback-resistant strains are roughly half of those observed in controls, suggesting a positive role played by a first enzyme with a normal but unoccupied feedback site. Some feedback-resistant mutants, in contrast to the wild type, were unable to exhibit derepression under histidine limitation caused by aminotriazole.
mesh_terms: D001276:ATP Phosphoribosyltransferase / Q000378:metabolism*; D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D004794:Enzyme Repression; D005246:Feedback; D006639:Histidine / Q000096:biosynthesis*; D006641:Histidinol / Q000494:pharmacology; D009154:Mutation; D009876:Operon; D010430:Pentosyltransferases / Q000378:metabolism*; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000201:enzymology / Q000378:metabolism*; D014230:Triazoles / Q000494:pharmacology
chemical_list: D012329:RNA, Bacterial; D012333:RNA, Messenger; D014230:Triazoles; D006639:Histidine; D006641:Histidinol; D010430:Pentosyltransferases; D001276:ATP Phosphoribosyltransferase; D000409:Alanine; D012293:Rifampin