[Optimization of a method for setting up the neuraminidase activity inhibition reaction (author's transl)].
Different conditions for neuraminidase activity inhibition test using ovomucine as the substrate were compared. The optimal results could be obtained after 1-hour contact of virus with antibody at 37 degrees C and after 18-hour contact of virus-antibody mixture with the substrate also at 37 degrees C. Antineuraminidase antibody titer may be calculated by the results of the test integration in comparison with virus control without correction for normal rabbit serum.
D000482:Allantois; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D004794:Enzyme Repression; D005260:Female; D007106:Immune Sera; D007158:Immunologic Techniques; D009439:Neuraminidase / Q000037:antagonists & inhibitors* / Q000276:immunology; D009975:Orthomyxoviridae / Q000201:enzymology; D010059:Ovomucin; D013696:Temperature; D013997:Time Factors; D014776:Virus Cultivation
D000914:Antibodies, Viral; D007106:Immune Sera; D010059:Ovomucin; D009439:Neuraminidase
Optimizatsiia metoda postanovki reaktsii podavleniia neĭraminidaznoĭ aktivnosti