Preparation of human lung biopsy specimens by perfusion-fixation.
A technique for perfusion-fixation of human lung biopsy specimens is described. The tissue is fixed in an expanded state by perfusing glutaraldehyde into cannulated blood vessels or bronchioles on the excisional surfaces. These specimens are ideally suited for histologic study and for both transmission and scanning electron microscopy.
D001706:Biopsy; D006652:Histological Techniques; D006801:Humans; D008168:Lung / Q000473:pathology / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D010477:Perfusion / Q000379:methods*