A spectrophotometric assay for gentamicin.
A rapid and accurate spectrophotometric assay has been developed for the determination of blood werum levels of gentamicin and related antibiotics. The assay uses a purified enzyme from Escherichia coli JR88/C600 that acetylates gentamicin with the production of coenzyme A, linked to a chemical reaction with a sulfhydryl reagent to produce stoichiometric amounts of a sensitive chromophore, monitored in the visible spectrum. The system provides advantages of speed, cost, convenience, accuracy, and enzyme stability to the desirable characteristics encountered with previous enzymatic methods.
D000123:Acetyltransferases / Q000378:metabolism; D004228:Dithionitrobenzoic Acid; D004926:Escherichia coli / Q000201:enzymology; D005839:Gentamicins / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D013053:Spectrophotometry / Q000379:methods*
D005839:Gentamicins; D004228:Dithionitrobenzoic Acid; D000123:Acetyltransferases