Automated single-column analysis of amino acids using ascorbic acid as reductant for air-stable ninhydrin.
The procedure for operation of a constant-temperature, single-column automated amino acid analyser in the sub-nanomole range is described. The cycle time for a complete analysis is 90 min including equilibration for next cycle. Eluting buffers can be made in the laboratory or commercially available concentrates (Pico-Buffers) can be used. A novel reducing agent, ascorbic acid, incorporated into the column buffers was used to reduce air-stable ninhydrin.
D000596:Amino Acids / Q000032:analysis*; D001205:Ascorbic Acid; D001322:Autoanalysis; D002021:Buffers; D002845:Chromatography; D004355:Drug Stability; D007192:Indenes; D008832:Microchemistry; D009555:Ninhydrin; D010084:Oxidation-Reduction; D013696:Temperature
D000596:Amino Acids; D002021:Buffers; D007192:Indenes; D009555:Ninhydrin; D001205:Ascorbic Acid