Organ culture of human skin in chemically defined medium.
Explants of adult human skin were grown in vitro in a chemically defined medium without serum. Under these conditions the epidermis was well maintained for up to 7 days, during which time new stratum corneum formation was observed. Most often the stratum corneum was orthokeratotic and associated with a prominent granular layer. Parakeratotic regions were also evident and these were not associated with a granular layer. During the first 5 days in culture there was a signficant increase in DNA synthesis (as measured radioautographically) and mitosis.
D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007642:Keratosis / Q000473:pathology; D008854:Microscopy, Electron; D008938:Mitosis; D009924:Organ Culture Techniques; D010241:Parakeratosis / Q000473:pathology; D012867:Skin / Q000166:cytology / Q000254:growth & development*
D003470:Culture Media; D004247:DNA