Improved micromethod for plasma fibrinogen unaffected by heparin therapy.
An improved micromethod for plasma fibrinogen, based on the Ellis-Stransky thrombin-clot density procedure, is presented. Modifications includes: (1) addition of Polybrene eliminates heparin interference; (2) measurement of absorbance at 340 nm provides improved sensitivity at low fibrinogen levels as well as with small volumes of plasma (40 mul.); (3) higher concentration of thrombin reduces the incubation time. Plasma fibrinogen by the proposed method compares well with a reference procedure based on total clottable protein (coefficient of correlation 0.99).
D001663:Bilirubin / Q000097:blood; D002940:Circadian Rhythm; D004211:Disseminated Intravascular Coagulation / Q000097:blood / Q000188:drug therapy; D005340:Fibrinogen / Q000032:analysis*; D006461:Hemolysis; D006493:Heparin / Q000627:therapeutic use; D006494:Heparin Antagonists / Q000008:administration & dosage; D006801:Humans; D006842:Hydrocarbons, Brominated / Q000008:administration & dosage; D008055:Lipids / Q000097:blood; D008832:Microchemistry; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D010783:Photometry / Q000379:methods*; D011073:Polyamines / Q000008:administration & dosage; D011108:Polymers / Q000008:administration & dosage; D013917:Thrombin; D013997:Time Factors
D006494:Heparin Antagonists; D006842:Hydrocarbons, Brominated; D008055:Lipids; D011073:Polyamines; D011108:Polymers; D005340:Fibrinogen; D006493:Heparin; D013917:Thrombin; D001663:Bilirubin