Cell wall of Fusarium sulphureum; I. Chemical composition of the hyphal wall.
The hyphae wall of Fusarium sulphureum Schlect. (Isolate 1) was isolated and purified. Electron microscopy studies showed that the isolated cell wall consisted of two distinct layers, an outer electron dense layer and a broader electron transparent inner layer. Chemical analysis revealed that the cell wall contained 66% carbohydrate, 7.3% protein, 5.5% lipid and 1.8% ash. The major cell wall component N-acetylglucosamine (39%) was shown by X-ray diffraction analysis to be present as chitin. Glucose constituted 14% of the cell wall, while mannose, galactose, and glucuronic acid, accounted for 15% of the cell wall. Glucuronic acid appears to be predominantly linked to galactose in the intact wall.
D000117:Acetylglucosamine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D002473:Cell Wall / Q000032:analysis* / Q000648:ultrastructure; D002686:Chitin / Q000032:analysis; D002849:Chromatography, Gas; D002854:Chromatography, Paper; D005670:Fusarium / Q000032:analysis* / Q000648:ultrastructure; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D005965:Glucuronates / Q000032:analysis; D008358:Mannose / Q000032:analysis; D008854:Microscopy, Electron; D014961:X-Ray Diffraction
D000596:Amino Acids; D005965:Glucuronates; D002686:Chitin; D005947:Glucose; D008358:Mannose; D000117:Acetylglucosamine; D005690:Galactose