Inhibitory effect of fluoride on glucose tests with glucose oxidase strips.
Sodium fluoride was inadvertently added as a preservative to the urine of an eight-year-old boy with diabetes mellitus before urinary glucose was measured. On preliminary screening of the urine, the test by glucose oxidase paper reagent strip gave a negative reading for glucose, whereas quantitative urinary glucose assay by the coupled enzyme reaction (hexokinase-glucose 6-phosphate dehydrogenase) gave a glucose concentration of 81.5 g/liter. Inadvertent use of sodium fluoride as a urine preservative may cause a falsely negative result with the glucose tests involving oxidase.
D002648:Child; D003920:Diabetes Mellitus / Q000175:diagnosis*; D005188:False Negative Reactions; D005459:Fluorides / Q000494:pharmacology*; D005949:Glucose Oxidase / Q000037:antagonists & inhibitors*; D006029:Glycosuria; D006593:Hexokinase; D006801:Humans; D008297:Male; D011934:Reagent Strips
D011934:Reagent Strips; D005949:Glucose Oxidase; D006593:Hexokinase; D005459:Fluorides