Hyperosmolar cold storage kidney preservative solution.
A hyperosmolar kidney preservative solution which can maintain kidneys from experimental animals viable for up to 72 hours is described.  Using the criterion of a one-month failure rate, the cold storage preservation method was found to be superior to machine preservation methods.  Sachs' solution was found to be superior to all other cold storage solutions.  The most important aspect of the hyperosmolar kidney preservative solution appears to be its ability to maintain normal intracellular electrolyte composition and to prevent cellular damage due to swelling.  The present formula requires that the basic solution and a magnesium chloride additive solution be prepared separately and combined before use.  A stable combined solution is proposed which can be sterilized by membrane filtration.
D000818:Animals; D001639:Bicarbonates; D001693:Biological Transport, Active; D003080:Cold Temperature; D004285:Dogs; D006982:Hypertonic Solutions; D007668:Kidney / Q000378:metabolism / Q000502:physiology; D008274:Magnesium; D009926:Organ Preservation; D013242:Sterilization; D014021:Tissue Preservation
D001639:Bicarbonates; D006982:Hypertonic Solutions; D008274:Magnesium