Regulation of homoserine transacetylase in whole cells of Bacillus polymyxa.
The levels of homoserine transacetylase (EC 2.3.1.31) in Bacillus polymyxa grown in minimal medium can vary over a 40-fold range, depending on whether methionine limits growth or is present in excess. This suggests that the synthesis of the enzyme is under control by methionine or one of its metabolites. The stability of homoserine transacetylase in growing cells was measured after repression of further synthesis by the addition of methionine. At 30 degrees, the enzyme was stable for 2 hours, whereas at 37 degrees it decayed with a half-life of 40 min. This contrasts with the striking instability in cell-free extracts described in the preceding paper (Wyman, A., and Paulus, H. (1975) J. Biol. Chem. 250, 3897-3903). The properties of homoserine transacetylase were also studied in cells of B. polymyxa that had been made permeable to small molecules by treatment with toluene. They differed in two important respects from those of the enzyme in cell-free extracts described in the preceding paper: the enzyme was relatively stable, with a half-life of 15 min at 37 degrees, and responded in a sigmoid manner to increasing concentrations of the inhibitors L-methionine and S-adenosylmethionine. These observations suggest that homoserine transacetylase is an oligomeric protein within the bacterial cell but dissociates into monomers in cell-free extracts. When B. polymyxa was transferred at 39 degrees from a rich medium to one without amino acids, growth resumed only very slowly. The growth lag after shift-down was not observed at 37 degrees or in the presence of methionine or cystathionine. This phenomenon appears to be due to a need for derepression of homoserine transacetylase upon shift-down which is thwarted at 39 degrees by the rapid thermal inactivation of the enzyme. A possible physiological function of the striking thermolability of the first enzyme in methionine biosynthesis is discussed.
D000123:Acetyltransferases / Q000378:metabolism*; D001407:Bacillus / Q000187:drug effects / Q000201:enzymology*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D004355:Drug Stability; D006714:Homoserine; D007700:Kinetics; D008715:Methionine / Q000494:pharmacology*; D012436:S-Adenosylmethionine / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014050:Toluene / Q000494:pharmacology
D003470:Culture Media; D014050:Toluene; D006714:Homoserine; D012436:S-Adenosylmethionine; D008715:Methionine; D000123:Acetyltransferases