Determination of the equilibrium constants of self-associating protein systems. XI. The application of C-(r) in graphical analysis and the enumeration of interacting species in the ultracentrifuge.
A greatly simplified procedure is proposed which employs C equals f(r) as determined from sedimentation equilibrium measurements in graphical analysis of self-associating protein systems and in the enumeration of interacting species in the ultracentrifuge. Basic equations given here are applicable to any self-associating system. A procedure is outlined for enumeration of interacting components independent of non-ideal behavior, using principal component analysis.
D001665:Binding Sites; D046911:Macromolecular Substances; D008433:Mathematics; D008970:Molecular Weight; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins
D046911:Macromolecular Substances; D011506:Proteins