[Demonstration by direct immunofluorescence of intracellular immunoglobulins on sections of tissues fixed in Bouin's fluid and embedded in paraplast].
Sections obtained from lymphoid tissues fixed immediately after biopsy in Bouin's fluid and embedded in paraplast may be used to visualize the immunosecretory cells with classical direct immunofluorescence. A comparative study of the same fragments processed according to the frozen section method followed by acetone fixation or according to Sainte-Marie's method confirms the value and the advantages of the proposed technique. Early fixation of thin fragments seems to be the main point.
D000818:Animals; D001706:Biopsy; D003593:Cytoplasm / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006041:Goats / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D007158:Immunologic Techniques; D008221:Lymphoid Tissue / Q000276:immunology*; D009369:Neoplasms / Q000276:immunology / Q000473:pathology
D007106:Immune Sera; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins
Mise en évidence des immunoglobulines intracellulaires par immunofluorescence directe sur coupe histologique aprés fixation par le liquide de Bouin et inclusion en paraplast