Sodium dodecyl sulfate in protein chemistry.
This review summarizes in a brief manner the main aspects of the application of sodium dodecyl sulfate (SDS) to protein chemistry. The principal problems of SDS-polyacrylamide gel electrophoresis are described, as well as the anomalous behavior of protein-SDS complexes and the inactivation of enzymes due to variable binding of SDS to the polypeptides studied. The particular value of SDS in elucidating the protein composition of biological membranes and in membrane-reconstitution experiments is discussed.
D002462:Cell Membrane / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000378:metabolism; D006358:Hot Temperature; D008970:Molecular Weight; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism / Q000494:pharmacology; D012997:Solvents
D004798:Enzymes; D011506:Proteins; D012997:Solvents; D012967:Sodium Dodecyl Sulfate