Glucocorticoid inhibition of protein synthesis in vivo and in vitro.
In young adult rats receiving glucocorticoids in pharmacological amounts, the accumulation proteins in the liver was suppressed. In vivo pulse-labeling experiments showed that inhibition of protein synthesis in the liver was an early effect of a single dose of glucocorticoid followed by a stimulation of protein synthesis. In animals receiving multiple doses of glucocorticoid over a period of a few days, a more pronounced inhibition in protein synthesis was observed, and the secondary stimulation of protein synthesis did not occur. These in vivo observations were paralleled by the results with cell-free protein-synthesizing systems, which indicated that the glucocorticoid inhibitory effect was imparted to the soluble fraction of the liver.
D000818:Animals; D002474:Cell-Free System; D004247:DNA / Q000096:biosynthesis; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000378:metabolism; D009929:Organ Size; D014176:Protein Biosynthesis; D051381:Rats; D013997:Time Factors
D004247:DNA; D007930:Leucine; D008775:Methylprednisolone