Electrophoretic analysis of the major polypeptides of human erythrocyte membranes prepared by low and high osmolarity haemolysis.
Human erythrocyte membranes were prepared in three ways: washing in hypotonic Tris buffer, pH 7.6, by lysis in isotonic Tris buffer pH 7.6 after incubation at 37 degrees C for 2 hours and by ultrasonication in an isotonic medium, pH 7.6. Analysis of the major polypeptides of the erythrocyte membranes by  sodium dodecylsulphate polyacrylamide gel electrophoresis revealed a selective depletion of a major polypeptide representing glyceraldehyde-3-phosphate dehydrogenase in the membranes prepared by high osmolarity lysis. The pattern of seperation of the remaining polypeptides was identical in the 3 different membrane preparations.
D001798:Blood Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000032:analysis* / Q000201:enzymology; D005069:Evaluation Studies as Topic; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000097:blood; D006461:Hemolysis; D006801:Humans; D008722:Methods; D009994:Osmolar Concentration; D010455:Peptides / Q000097:blood*
D001798:Blood Proteins; D010455:Peptides; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases