Demonstration of immunoglobulin containing deposits in glomerular basement membrane in experimental chronic serum sickness using horseradish peroxidase labelled antiserum.
Horseradish peroxidase (HRP) labelled antibodies were used in rabbit glomerulonephritis to obtain localization of immunoglobulins in the glomerulus at a light and electron microscope level. Better localization of deposits was obtained with peroxidase labelled antiserum than with immunofluorescence at the light microscope level. Ultrastructurally, immunoglobulin localization was seen to correspond to the electron dense deposits demonstrated in and on the glomerular basement membrane by conventional microscopy.
D000818:Animals; D001485:Basement Membrane / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000150:complications / Q000276:immunology; D006735:Horseradish Peroxidase / Q000276:immunology; D007106:Immune Sera; D007136:Immunoglobulins / Q000032:analysis*; D007678:Kidney Glomerulus / Q000276:immunology*; D008854:Microscopy, Electron; D011507:Proteinuria / Q000150:complications / Q000276:immunology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D012713:Serum Sickness / Q000276:immunology*
D007106:Immune Sera; D007136:Immunoglobulins; D012710:Serum Albumin, Bovine; D006735:Horseradish Peroxidase