title: Studies on a temperature sensitive nuclear petite mutant of Saccharomyces cerevisiae: phenotypic reversibility of the mitochondrial functions.
abstract: 1. We have studied the pleiotropic effect of a single-gene mutation of the pts mutant strain 1511 grown at 23 degrees C and 36 degrees C. 2. Growth of the mutant at the non-permissive temperature results in a decrease of respiration rate to about 50% after one generation and to less than 5% after five generations. The cytochrome spectra analysis revealed that only cytochrome c was present after growth at 36 degrees C. 3. Mitochondrial protein synthesis experiments in vivo demonstrated that the protein synthesizing system was not as rapidly inactivated by high temperature as the respiratory system. 4. The recovery of the respiratory capacity of the cells at 23 degrees C is complete but dependent on the de novo synthesis of a temperature sensitive protein.
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D004917:Erythromycin / Q000494:pharmacology; D005111:Extrachromosomal Inheritance; D008928:Mitochondria / Q000378:metabolism*; D009154:Mutation; D010101:Oxygen Consumption / Q000187:drug effects; D010641:Phenotype; D014176:Protein Biosynthesis; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure; D013696:Temperature
chemical_list: D003580:Cytochromes; D004917:Erythromycin