[A microfluorometric study of cell nucleus DNP-complex renaturation].
A study of the kinetics of renaturation of the DNP in the nuclei of human peripheral blood lymphocytes with the aid of modified Riglers method showed the absence of renaturation of the preparations preliminarily headed to 65 degrees C. Heating the preparations to higher temperatures (75, 85 and 100 degreesC) led to the appearance of characteristic renaturation profiles with the maximum at a two-minute incubation (direct measurement of the intensity of fluorescence of the nuclei of cells transfered into a cold citrate-salt solution, as a time function).
D000166:Acridines; D004247:DNA; D005470:Fluorometry; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes; D009695:Nucleic Acid Renaturation; D009696:Nucleic Acids; D009698:Nucleoproteins
D000166:Acridines; D009696:Nucleic Acids; D009698:Nucleoproteins; D004247:DNA
Issledovanie renaturatsii DNP-kompleksa kletochnykh iader mikrofliuorimetricheskim metodom