Synthesis of certain puromycin analogues and their use in studying the peptidyl synthetase enzyme of e. coli and rat liver ribosomes.
The beta-alanyl and L-histidyl analogues of puromycin were synthesized chemically and tested for their ability to release [3H] N-acetylphenylalanine from its tRNA carrier in the rat liver and E. Coli ribosomal systems. Both analogues were found to be inactive in releasing [3H] N-acetylphenylalanine. Reasons for the inactivity of these compounds are discussed in relation to the structure of the puromycin molecule and the requirements for puromycin-like activity.
D000818:Animals; D004926:Escherichia coli / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D010453:Peptide Synthases / Q000378:metabolism*; D010649:Phenylalanine / Q000031:analogs & derivatives; D011691:Puromycin / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D012343:RNA, Transfer; D051381:Rats; D012270:Ribosomes / Q000201:enzymology*; D013329:Structure-Activity Relationship
D010649:Phenylalanine; D011691:Puromycin; D012343:RNA, Transfer; D010453:Peptide Synthases