Induction of error-prone repair as a consequence of DNA ligase deficiency in Escherichia coli.
DNA ligase deficiency is shown to induce generalized mutator activity in E. coli. This mutator activity is unaffected by 3 mug/ml of chloramphenicol but is abolished both in lig-recA double mutants and by incubation with 20 mug/ml of chloramphenicol. Dna ligase deficiency is also shown to reactivate ultraviolet light-irradiated phage lambda and T7 and to increase both spontaneous and ultraviolet light-induced mutagenesis in phage lambda, all of which are abolished in lig-recA strains. Interaction occurs between the molecular events of mutagenesis induced by ultraviolet irradiation and those induced by DNA ligase deficiency. These observations suggest a common pathway, coordinately expressed with the inducible repair mode, that is responsible for mutagenesis.
D002701:Chloramphenicol / Q000494:pharmacology; D003090:Coliphages / Q000528:radiation effects; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000201:enzymology* / Q000528:radiation effects; D009154:Mutation / Q000187:drug effects; D011118:Polynucleotide Ligases / Q000378:metabolism*; D011830:Radiation Effects; D013696:Temperature; D014466:Ultraviolet Rays
D002701:Chloramphenicol; D011118:Polynucleotide Ligases