title: Radioimmunoassay for motilin.
abstract: A specific radioimmunoassay for motilin has been developed with the use of antisera to porcine motilin raised in guinea pigs. Highly purified 125-I-motilin was used as the tracer and the sensitivity range was 10 to 320 pg. No cross-reactivity was demonstrated with gastric inhibitory polypeptide, secretin, glucagon, gastrin, cholecystokinin-pancreozymin, or vasoactive intestinal peptide. In dogs with denervated pouches of the fundus of the stomach and Mann-Bollman fistulae, duodenal alkalinization resulted in an increase in gastric motor activity in the fundic pouch with a corresponding increase in serum motilin.
mesh_terms: D000818:Animals; D000941:Antigens; D002417:Cattle / Q000276:immunology; D002766:Cholecystokinin / Q000097:blood / Q000276:immunology; D003429:Cross Reactions; D004285:Dogs; D005755:Gastrins / Q000097:blood / Q000276:immunology; D005768:Gastrointestinal Hormones / Q000097:blood / Q000276:immunology*; D005934:Glucagon / Q000097:blood; D006168:Guinea Pigs / Q000276:immunology; D006728:Hormones / Q000097:blood / Q000276:immunology; D006801:Humans; D007457:Iodine Radioisotopes; D010455:Peptides / Q000276:immunology*; D011863:Radioimmunoassay; D012633:Secretin / Q000097:blood / Q000276:immunology; D013552:Swine
chemical_list: D000941:Antigens; D005755:Gastrins; D005768:Gastrointestinal Hormones; D006728:Hormones; D007457:Iodine Radioisotopes; D010455:Peptides; D012633:Secretin; D005934:Glucagon; D002766:Cholecystokinin