Localization of polycyclic hydrocarbon carcinogens in the lung following intratracheal instillation in gelatin solution.
The deposition and localization of benzo(a)pyrene (BP) suspended in a gelatin-0.9 percent NaCl solution was studied in hamster lungs by ultraviolet fluorescence microscopy. BP was deposited primarily in the alveolar region of the lung. Although large numbers of BP-filled macrophages were seen in the upper airways by 24 hr after an instillation, little BP appeared to penetrate into the bronchial epithelium. The intratracheal instillation of polycyclic hydrocarbons in a gelatin-0.9 percent NaCl solution appears to be a useful model when it is desired to deliver the carcinogen dose to the peripheral lung.
D000818:Animals; D001580:Benzopyrenes / Q000008:administration & dosage / Q000378:metabolism; D001980:Bronchi / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism*; D006224:Cricetinae; D005780:Gelatin; D006838:Hydrocarbons / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D014677:Pharmaceutical Vehicles; D011650:Pulmonary Alveoli / Q000378:metabolism; D013997:Time Factors
D001580:Benzopyrenes; D002273:Carcinogens; D006838:Hydrocarbons; D014677:Pharmaceutical Vehicles; D005780:Gelatin