Selection of chinese hamster somatic cell mutants after irradiation of BUdR-labelled cells.
The selection of somatic cell mutants after irradiation of BUdR-labelled cells is improved with the use of a mercury lamp combined with a plastic sheet which filters the unwanted wave lengths below 303 nm. New somatic cell mutants defective for galactose metabolism or auxotrophe for fetal calf serum were thus isolated.
D001973:Bromodeoxyuridine / Q000378:metabolism*; D002460:Cell Line; D003584:Cytological Techniques / Q000295:instrumentation; D004247:DNA / Q000032:analysis; D005690:Galactose / Q000378:metabolism; D009154:Mutation; D011830:Radiation Effects; D006599:UDPglucose-Hexose-1-Phosphate Uridylyltransferase / Q000378:metabolism; D014466:Ultraviolet Rays
D004247:DNA; D006599:UDPglucose-Hexose-1-Phosphate Uridylyltransferase; D001973:Bromodeoxyuridine; D005690:Galactose