Evaluation of excitation light sources for incident immunofluorescence microscopy.
A variety of fluorescent excitation light sources were compared using a standard fluorescein solution or a bacterial conjugate with immunofluorescent microscopy. Quantitative data were obtained with microscope photometric apparatus. Both the quantitative data and comparative conjugate titering suggest that the 450-W xenon arc excited significantly more fluorescence than did the more commonly used 250-W mercury arc or the 100-W halogen lamp. The conjugate could be diluted 4 to 32 times more using the 450-W xenon. Additional advantages of 450-W xenon excitation include sufficient energy of wave lengths between 470 to 490 mm, thus permitting narrow-band excitation resulting in less autofluorescence and the ability to perform fluorescent-antibody procedures without the darkening of ambient room light.
D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006219:Halogens; D008027:Light; D008628:Mercury; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D014978:Xenon
D006219:Halogens; D014978:Xenon; D008628:Mercury