[Fluorimetric determination of testosterone on Al2O3 by thin-layer chromatographic separation of the trimethylsilyl ether derivatives (author's transl)].
Small quantities (parts per 10-9) of testosterone were determined in the presence of large amounts of other steroids in urine samples obtained during pregnancy by subjecting their trimethylsilyl derivatives to spectrofluorimetry on alumina after purification by thin-layer chromatography on silica gel. The activity of the alumina and the fluorescence reactions are discussed.
D000535:Aluminum; D002855:Chromatography, Thin Layer; D005260:Female; D005782:Gels; D006801:Humans; D008722:Methods; D011247:Pregnancy; D013050:Spectrometry, Fluorescence; D013739:Testosterone / Q000652:urine*; D014297:Trimethylsilyl Compounds
D005782:Gels; D014297:Trimethylsilyl Compounds; D013739:Testosterone; D000535:Aluminum
Fluorimetrische Testosteronbestimmung auf Al2O3 durch Dünnschichtchromatographische Trennung der Trimethylsilyläther