Isolation and identification of prostaglandin E2 from gastrointestinal tract of shark Triakis scyllia.
A prostaglandin was isolated from the gastrointestinal tract of the shark Triakis scyllia and identified as prostaglandin E2 by bioassay, thin-layer chromatography, gas-liquid chromatography, ultraviolet absorption spectrometry and mass spectrometry. The fatty acid composition of the tissue was also determined. The amount of eicosatetraenoic acid in this tissue was 17.2% of the total fatty acids, but the percentages of eicosatrienoic acid and eicospentaenoid acid were low.
D000818:Animals; D001681:Biological Assay; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004064:Digestive System / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D013058:Mass Spectrometry; D011453:Prostaglandins / Q000302:isolation & purification; D011458:Prostaglandins E / Q000032:analysis*; D012754:Sharks; D013056:Spectrophotometry, Ultraviolet
D005227:Fatty Acids; D011453:Prostaglandins; D011458:Prostaglandins E