Isolation and characterization of two hydroxyproline-containing glycoproteins from normal animal lung lavage and lamellar bodies.
Two glycopeptides, present in particulate material obtained by pulmonary lavage from normal rabbits, were isolated and characterized. The same two glycopeptides were present in preparations of lamellar bodies from rabbit lung. The estimated molecular weights of the two glycopeptides by sodium dodecyl sulfate-acrylamide gel electrophoresis were found to be 62,000 and 36,000 and both were found to contain hydroxyproline and relatively high amount of glycine (11 and 15 per cent, respectively). Carbohydrate analysis of the two glycopeptides demonstrated the presence of glucosamine, sialic acid, mannose, Fucose, and galactose. Similar glycopeptides of the same molecular weights, and amino acid and carbohydrate compositions have been found in layage mateial isolated from lungs of patients with alveolar proteinosis. The data indicate that thses two collagen-like glycopeptides are major intra-alveolar proteins in many mammals, including humans.
D000596:Amino Acids; D000818:Animals; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose; D005690:Galactose; D005944:Glucosamine; D006023:Glycoproteins / Q000302:isolation & purification*; D006909:Hydroxyproline; D007525:Isoelectric Focusing; D008168:Lung / Q000032:analysis*; D008358:Mannose; D011650:Pulmonary Alveoli / Q000032:analysis; D011817:Rabbits; D012794:Sialic Acids; D012967:Sodium Dodecyl Sulfate; D013395:Sucrose
D000596:Amino Acids; D006023:Glycoproteins; D012794:Sialic Acids; D005643:Fucose; D012967:Sodium Dodecyl Sulfate; D013395:Sucrose; D005944:Glucosamine; D008358:Mannose; D006909:Hydroxyproline; D005690:Galactose