Heterogeneous nuclear rna from lymphocytes of chronic lymphocytic leukaemia: adenylate-rich and double-stranded regions.
Rapidly labelled high molecular weight nuclear RNA from lymphocytes of chronic lymphocytic leukaemia was analysed for ribonuclease-stable adenylate-rich and double-stranded regions. The polyadenylate content corresponds to 0.4-0.5 percent and the content of double-stranded sequences to 2-4 percent of the total nucleotides. Partial association of polyadenylate segments with double-stranded regions was found by comparative analysis of (3H)-adenosine and (3H)-uridine labelled ribonuclease-stable RNA before and after thermal denaturation. Comparison with normal lymphocytes shows lower proportions of polyadenylate-containing RNA binding to poly(U)-Sepharose in leukaemia cells than in normals. Partial degradation of rapidly labelled high molecular weight RNA was found in leukaemia cases with low white cell counts.
D000241:Adenosine / Q000032:analysis; D007945:Leukemia, Lymphoid; D008214:Lymphocytes / Q000032:analysis*; D008970:Molecular Weight; D012313:RNA / Q000032:analysis*; D012260:Ribonucleases; D014529:Uridine / Q000032:analysis
D012313:RNA; D012260:Ribonucleases; D000241:Adenosine; D014529:Uridine