Gram-scale purification of methionyl-tRNA and tyrosyl-tRNA synthetases from Escherichia coli.
A procedure is presented for the purification of 3 g of homogeneous methionyl-tRNA synthetase and 1 g of homogeneous tyrosyl-tRNA synthetase from 50 kg batches of Escherichia coli. The procedure permits the isolation of many enzymes simultaneously and the elution positions of seven other aminoacyl-tRNA synthetases, catalase, rhodanese, phosphofructokinase, elongation factor Tu and cytochrome b-562 are indicated. The problems of extraction work on this scale are discussed.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology*; D006882:Hydroxyapatites; D008718:Methionine-tRNA Ligase / Q000302:isolation & purification*; D014448:Tyrosine-tRNA Ligase / Q000302:isolation & purification*
D006882:Hydroxyapatites; D000604:Amino Acyl-tRNA Synthetases; D014448:Tyrosine-tRNA Ligase; D008718:Methionine-tRNA Ligase