A new reversed passive hemagglutination test for detection of HBsAg.
A new reversed passive hemagglutination test for HBsAg, termed Raphadex B, has been developed using immunochemically purified chimpanzee anti-HBs bound to stabilized human erythrocytes. The test has been found to have equivalent sensitivity to the Ausria 125I radioimmunoassay, and detected a similar number of HBsAg-containing specimens in screening of volunteer blood donors. This method offers an economical approach to third generation methodology for hepatitis B screening of blood donors.
D000017:ABO Blood-Group System; D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D000956:Antigens, Viral / Q000008:administration & dosage; D001782:Blood Donors; D002986:Clinical Trials as Topic; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests / Q000379:methods*; D006511:Hepatitis B Antigens / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D007273:Injections, Intramuscular; D009500:Neutralization Tests; D002679:Pan troglodytes
D000017:ABO Blood-Group System; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006511:Hepatitis B Antigens