[The effect of diphtheria toxin on proliferation of a transplantable culture of human amnion cells].
Cytoxic effect (CTE) of diphtheria toxin (DT) is preceded by disturbances of proliferation process, this being expressed in reduction of the proliferation index (PI) and of mitotic index (MI) before the appearance of the CTE. The effect of the toxin on the proliferation and mitoses of the cell culture is expressed after the incubation period of about 3 hours. The extent and the rate of reduction of the indices directly depended on the dose of the toxin whose low doses at the early periods stimulated the proliferation. The maximum of all the manifestations of the toxin action coincided with the period of increased proliferation in control. On the basis of investigations carried out a hypothesis was put forward that the CTE of diphtheria toxin developed with the entrance of the cells into mitosis.
D000650:Amnion; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D004167:Diphtheria Toxin / Q000633:toxicity*; D006801:Humans; D008938:Mitosis; D013997:Time Factors
D004167:Diphtheria Toxin
Vliianie difteriĭnogo toksina na proliferatsiiu perevivaemoĭ kul'tury amnioticheskikh kletok cheloveka