Erythropoiesis in steel mutant mice: effects of erythropoietin in vitro.
Adult SI/SI-d mutant mice have severe macrocytic, normochromic anemia. Moreover these animals are unresponsive to the stimulation of erythropoietin in vivo. By means of a bone marrow cell suspension culture system, the present investigation shows that in adult SI/SI-d marrow, there are cells capable of responding in vitro to erythropoietin in a normal fashion. Moreover, the erythropoietin present in SI/SI-d serum is biologically active in vitro without any prior biochemical modification. These observations support the suggestion that there is a defect in differentiation in the erythroid cell lines of SI/SI-d mice in vivo due to an abnormal hemopoietic microenvironment.
D000748:Anemia, Macrocytic / Q000097:blood; D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004920:Erythropoiesis; D004921:Erythropoietin / Q000502:physiology*; D005269:Femur; D006418:Heme / Q000096:biosynthesis; D007504:Iron Radioisotopes; D051379:Mice; D009154:Mutation; D012756:Sheep
D007504:Iron Radioisotopes; D004921:Erythropoietin; D006418:Heme