Egasyn, a protein complexed with microsomal beta-glucuronidase.
Two polypeptide chains are present in murine beta-glucuronidase precipitated with a specific anti-beta-glucuronidase antibody F(ab)2 fragment. One is the catalytic subunit of beta-glucuronidase and the other has the properties predicted for the hypothetical beta-gluronidase membrane anchor protein. The new protein, named egasyn, is associated with microsomal, but no lysosomal beta-glucuronidase. It is released from the microsomal beta-glucuronidase complex by heat treatment. The YBR strain of mice carrying the Eg degrees mutation does not form an egasyn-beta-glucuronidase complex and is unable to retain beta-glucuronidase on microsomal membranes.
D000818:Animals; D002265:Carboxylic Ester Hydrolases; D004591:Electrophoresis, Polyacrylamide Gel; D005966:Glucuronidase / Q000276:immunology / Q000378:metabolism; D006041:Goats / Q000276:immunology; D007140:Immunoglobulin Fab Fragments; D007527:Isoenzymes / Q000276:immunology / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000201:enzymology*; D008970:Molecular Weight; D009154:Mutation; D011233:Precipitin Tests; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism
D007140:Immunoglobulin Fab Fragments; D007527:Isoenzymes; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D011506:Proteins; D002265:Carboxylic Ester Hydrolases; C009751:egasyn; D005966:Glucuronidase