Differential binding to Sepharose-Con A of B12-binding proteins from human gastric juice.
Human gastric juice was found to contain at least two vitamin B-12 binding substances.  One of the proteins which formed a complex with B12 was found to bind to a column containing Sepharose-ConA.  Since the protein which bound to Sepharose-ConA was absent in the gastric juice of pernicious anemia patients it was concluded that this protein was intrinsic factor.  The ability of intrinsic factor to bind to Sepharose-ConA offers a potential means by which intrinsic factor could be separated from other B-12 binding proteins in gastric juice.  The ConA binding properties of intrinsic factor might be exploited in the development of a diagnostic test for pernicious anemia.
D000752:Anemia, Pernicious / Q000175:diagnosis / Q000378:metabolism; D002846:Chromatography, Affinity; D003208:Concanavalin A; D004586:Electrophoresis; D005260:Female; D005750:Gastric Juice / Q000032:analysis*; D006801:Humans; D007437:Intrinsic Factor / Q000032:analysis*; D008297:Male; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D012685:Sepharose; D014805:Vitamin B 12 / Q000378:metabolism
D011506:Proteins; D003208:Concanavalin A; D007437:Intrinsic Factor; D012685:Sepharose; D014805:Vitamin B 12