Changes in insulin content of, and insulin and RNA synthesis in, isolated rat islets of Langerhans after hypophysectomy and short-term growth hormone replacement.
Three to four weeks after hypophysectomy, the insulin content and glucose-stimulated insulin or total RNA synthesis in the pancreatic islets were estimated. The ratio of the glucose-stimulated insulin release to the islet insulin content was lowered. After short-term GH replacement, insulin release and insulin content were slightly increased such that the ratio of release to content remained virtually unaltered. Glucose-stimulated insulin synthesis was also depressed but improved slightly after GH replacement. However, there was no significant change in the incorporation of [5-3H]uridine into islet total RNA after hypophysectomy or subsequent GH replacement.
D000818:Animals; D002417:Cattle; D003609:Dactinomycin / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D013006:Growth Hormone / Q000494:pharmacology*; D007016:Hypophysectomy; D066298:In Vitro Techniques; D007328:Insulin / Q000096:biosynthesis / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010902:Pituitary Gland / Q000502:physiology*; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D013997:Time Factors; D014498:Uracil / Q000378:metabolism; D014500:Uracil Nucleotides / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D007328:Insulin; D014500:Uracil Nucleotides; D003609:Dactinomycin; D014498:Uracil; D012313:RNA; D013006:Growth Hormone; D005947:Glucose; D014529:Uridine