Metabolism of long-chain isoprenoid alcohols. Incorporation of phytol and dihydrophytol into the lipids of rat brain.
[U-14-C]Phytol (3,7,11,15-tetramethylhexadec-2-en-1-ol) and [U-14-C]dihydrophytol (3,7,11,15-tetramethylhexadecanol) were administered intracerebrally to 18-day-old rats and incorporation of radioactivity into brain lipids was determined after 6 and 24 h. Radioactivity from [U-14-C]phytol was found in free phytenic (3,7,11,15-tetramethylhexadec-2-enoic), phytanic (3,7,11,15-tetramethylhexadecanoic) and pristanic (2,6,10,14-tetramethylpentadecanoic) acids, in phytanic and pristanic acid moieties of neutral and polar lipids, and in esters of phytol. In addition, evidence is presented for the utilization of phytol to form 1-O-phytenyl-2-acyl glycerophosphatides. Radioactivity from [U-14-C]dihydrophytol was found in free phytanic and pristanic acids, the corresponding acyl groups of neutral and polar lipids, esters of dihydrophytol and 1-O-phytanyl-2-acyl glycerophosphatides. Incorporation of either substrate into O-alkylglycerols was very low, and labeled branched-chain alk-1-enylglycerols could not be detected.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004224:Diterpenes / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D050356:Lipid Metabolism; D008297:Male; D010743:Phospholipids / Q000378:metabolism; D010836:Phytol / Q000031:analogs & derivatives / Q000378:metabolism*; D051381:Rats; D014280:Triglycerides / Q000378:metabolism; D014885:Waxes / Q000378:metabolism
D002250:Carbon Radioisotopes; D004224:Diterpenes; D005230:Fatty Acids, Nonesterified; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D014280:Triglycerides; D014885:Waxes; D010836:Phytol