Aging and the passage of L-tyrosine, L-DOPA, and inulin into mouse brain slices in vitro.
The effect of age on active transport of catecholamine precursors was studied in a healthy population of mature (12-mo.-old) and senescent (30-mo.-old) C57BL/6J male mice. No age differences were observed in uptake of L-(3H)-tyrosine IN VITRO by slices of hypothalamus, brain stem, and striatum at external concentrations equal to or greater than blood levels. However, age-related reductions were observed intermittently in the hypothalamus, brain stem, and striatum at very low concentrations of tyrosine (less than 10 pg/ml). No age differences were observed in incorporation of L-(3H)-tyrosine into hypothalamic proteins when corrected for intracellular uptake of L-(3H)-tyrosine. No age differences were observed in uptake of L-(3H)-DOPA. The possible contribution of age-related changes in extra-cellular space to decreased uptake was determined to be negligible by wash-out studies of tyrosine and by the uptake of inulin. A significant age-related increase of inulin uptake IN VITRO was observed under ionic conditions which minimize tissue swelling.
D000375:Aging; D000818:Animals; D001693:Biological Transport, Active; D001921:Brain / Q000378:metabolism*; D001933:Brain Stem / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D066298:In Vitro Techniques; D007444:Inulin / Q000378:metabolism*; D007980:Levodopa / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014316:Tritium; D014443:Tyrosine / Q000378:metabolism*
D014316:Tritium; D014443:Tyrosine; D007980:Levodopa; D007444:Inulin