title: Regulation of synthesis of ribosomal proteins during pyrimidine starvation in Escherichia coli.
abstract: The synthesis of ribosomal proteins during pyrimidine starvation was investigated. A progressive turn-off of protein synthesis, with a decay half-time of about 5 min, was observed when Escherichia coli cells were starved of uridine. By means of double-labelling, the syntheses of different ribosomal proteins were shown to be turned off unequally during the starvation. Comparison of the turn-off patterns for some proteins and the known polycistronic organization of the structural genes for these proteins suggests that a major cause of the unequal turn-off was the degradation of mRNA molecules for the ribosomal proteins from the 5'-end toward the 3'-end.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism*; D005796:Genes; D007930:Leucine / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012269:Ribosomal Proteins / Q000096:biosynthesis*; D014529:Uridine / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D012313:RNA; D007930:Leucine; D014529:Uridine