title: Susceptibility of a hybrid plasmid to excision of genetic material.
abstract: A 5 Megadalton segment of DNA carrying a gene for kanamycin resistance from R447 b (a plasmid of group N of molecular weight 33 Megadaltons) has been inserted into Plac (a plasmid of the A--C complex of molecular weight 101 Megadaltons) to produce the recombinant plasmid Plac-R447 b (Coetzee, 1974). The recombinant plasmid is a typical member of the A--C complex except that entry of an N group plasmid into a Plac-R447 b+ recipient frequently leads to the loss of 5 Megadaltons of DNA (including the kanamycin resistance determinant) from the resident plasmid. In those transcipients from which kanamycin resistance is not eliminated, both plasmids are stably inherited.
mesh_terms: D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000378:metabolism; D004352:Drug Resistance, Microbial; D005111:Extrachromosomal Inheritance; D006824:Hybridization, Genetic; D007612:Kanamycin / Q000494:pharmacology; D008970:Molecular Weight; D010957:Plasmids; D011513:Proteus mirabilis / Q000187:drug effects / Q000502:physiology*; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial; D007612:Kanamycin