Protein composition of nuclear 14 S ribonucleoprotein particles containing poly (A).
Nuclear 14 S RNP particles containing poly (A) from Ehrlich ascites carcinoma cells and rat liver were purified by re-sedimentation in sucrose gradients, by Cs2SO4 density gradient centrifugation and by affinity chromatography on a poly (dT)-Sepharose column. Proteins of these RNP particles were electrophoresed in urea and SDS-polyacrylamide gels. RNP particles of ascites carcinoma cells contain two main bands having molecular weights of 51 000 and 69 000 daltons, respectively, and two or three minor components.
D000227:Adenine Nucleotides / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*; D009698:Nucleoproteins / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D051381:Rats; D014508:Urea
D000227:Adenine Nucleotides; D009363:Neoplasm Proteins; D009698:Nucleoproteins; D012334:RNA, Neoplasm; D014508:Urea