title: [Determination of iron with CentrifiChem System].
abstract: The determination of iron was adapted to the CentrifiChem System. The iron bound to transferrin is freed with a detergent, reduced to Fe++ with sodium dithionite, and determined with bathophenanthroline disulphonate. The operation consists of one run for the blank value and one analytical run. Although the actual reaction time is extremely short, a reaction time of 8--10 min is recommended for both the blank value and the analysis. This ensures adequate clearing during centrifugation under the influence of Teepol. 2 times 100 mul serum are required, and 80 determinations per hour are possible.
mesh_terms: D001322:Autoanalysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003124:Colorimetry; D004227:Dithionite; D007501:Iron / Q000097:blood*; D010084:Oxidation-Reduction; D010618:Phenanthrolines; D014168:Transferrin / Q000378:metabolism
chemical_list: D010618:Phenanthrolines; D014168:Transferrin; D004227:Dithionite; D007501:Iron
vernacular_title: Eisen-Bestimmung mit dem CentrifiChem System