Application of pulse radiolysis to the study of drug interactions with biological macromolecules.
Pulse radiolysis has been used to study the interactions of a variety of small molecules with DNA. The diffusion-controlled rate constants for the reactions of e(aq) with dyes, cations and other small molecules including radioprotectors and radiosensitizers, were found to decrease remarkably upon association with DNA. These kinetic data can be used to quantitate the degree and extent of such interactions. This technique may have wide practical application for the in situ study of many small molecule-macromolecule associations, under physiological conditions.
D000963:Antimetabolites / Q000494:pharmacology; D001665:Binding Sites; D002413:Cations, Divalent; D004396:Coloring Agents / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects*; D004058:Diffusion; D004996:Ethidium / Q000528:radiation effects*; D007700:Kinetics; D008722:Methods; D009690:Nucleic Acid Conformation; D004364:Pharmaceutical Preparations / Q000528:radiation effects*; D011830:Radiation Effects; D013053:Spectrophotometry; D013997:Time Factors
D000963:Antimetabolites; D002413:Cations, Divalent; D004396:Coloring Agents; D004364:Pharmaceutical Preparations; D004247:DNA; D004996:Ethidium