Fluorescamine as a terminating agent in solid phase peptide synthesis.
Fluorescamine was shown to be an excellent terminating agent for blocking unreacted amino groups during solid phase peptide synthesis. A comparison of the termination efficiency of fluorescamine versus that of acetylation revealed that the former method gave superior products as assessed by peptide analysis, dansyl-amino end group determination and biological assay. In addition, fluorescamine terminated fragments were converted to non-fluorescent spirolactones during the deprotection stage. These spirolactones were stable to subsequent solid phase reaction conditions and were readily removed from the target peptide.
D000107:Acetylation; D000596:Amino Acids / Q000032:analysis; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001572:Benzofurans / Q000378:metabolism; D001681:Biological Assay; D002855:Chromatography, Thin Layer; D003106:Colon / Q000378:metabolism; D046508:Culture Techniques; D004285:Dogs; D004590:Electrophoresis, Paper; D005260:Female; D007202:Indicators and Reagents; D008297:Male; D010452:Peptide Biosynthesis; D010455:Peptides / Q000032:analysis; D051381:Rats; D013141:Spiro Compounds / Q000378:metabolism*
D000596:Amino Acids; D001572:Benzofurans; D007202:Indicators and Reagents; D010455:Peptides; D013141:Spiro Compounds; D000804:Angiotensin II