Irreversible binding of carbon tetrachloride to microsomal phospholipids. Free radical nature of the reactive specie and alterations in the physico-chemical properties of the target fatty acids.
Thin layer and gas liquid chromatographic analysis of the fatty acid methyl esters derived from liver microsomal phosphatidylcholine from animals treated with 14CC1-4 revealed a very similar composition to that observed during the analysis of the reaction products arised by the in vitro benzoyl peroxide initiated CC1-4 chemical addition to double bonds of fatty acid methyl esters or to fatty acids in phospholipids later transformed to methyl esters. The well known CC1-3 free radical nature of this in vitro chemical reaction gives support to the possibility for a CC1-3 free radical formation in vivo.
D000818:Animals; D002250:Carbon Radioisotopes; D002251:Carbon Tetrachloride / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005609:Free Radicals; D066298:In Vitro Techniques; D008099:Liver / Q000648:ultrastructure; D008862:Microsomes, Liver / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
D002250:Carbon Radioisotopes; D005231:Fatty Acids, Unsaturated; D005609:Free Radicals; D010713:Phosphatidylcholines; D010743:Phospholipids; D002251:Carbon Tetrachloride