Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2).
Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible function of this DNA is discussed.
D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004270:DNA, Circular / Q000302:isolation & purification*; D005111:Extrachromosomal Inheritance; D008854:Microscopy, Electron; D008970:Molecular Weight; D009154:Mutation; D010761:Phosphorus Radioisotopes; D013302:Streptomyces / Q000032:analysis*; D013936:Thymidine; D014316:Tritium
D004269:DNA, Bacterial; D004270:DNA, Circular; D010761:Phosphorus Radioisotopes; D014316:Tritium; D013936:Thymidine