title: The contribution of RNA and non-histone proteins to the circular dichroism spectrum of chromatin.
abstract: This paper is an investigation of the contribution of low salt extractable RNA and non-histone proteins to the circular dichroism of chromatin. Circular dichroism (CD) of chromatin above 250 nm is due mainly to DNA and is different from that of DNA free in solution. In addition, to a smaller extent, we find that low salt extractable RNA and/or non-histone protein side chain chromophores contribute significantly to the spectra in this region and account for the major differences observed among the CD spectra of chromatins isolated from the five tissues studied; pig cerebellum, myeloma, calf thymus, chick embryo brain, and chick erythrocytes.
mesh_terms: D000818:Animals; D001665:Binding Sites; D001923:Brain Chemistry; D002417:Cattle; D002531:Cerebellum; D002642:Chick Embryo; D002645:Chickens; D002843:Chromatin / Q000032:analysis; D002942:Circular Dichroism; D004912:Erythrocytes; D009101:Multiple Myeloma; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins; D011485:Protein Binding; D011487:Protein Conformation; D012313:RNA; D013053:Spectrophotometry; D013552:Swine; D013950:Thymus Gland
chemical_list: D002843:Chromatin; D009698:Nucleoproteins; D012313:RNA