title: Inhibition of nucleoside Q formation in transfer ribonucleic acid during methionine starvation of relaxed-control Escherichia coli.
abstract: The elution profiles of Asp-tRNA from unstarved and starved cultures of a relaxed-control (Rel-) strain of Escherichia coli were compared by reversed-phase chromatography. Methionine starvation results in the appearance of several additional species of Asp-tRNA which are not observed with starvation for leucine or histidine. By the criterion of cyanogen bromide-effected shifts in chromatographic elution position, a large portion of the tRNAAsp synthesized in methionine-starved cells lacks the normal Q nucleoside. By the same criterion, virtually all of the tRNAAsp from unstarved, leucine-starved, and histidine-starved cells contain Q. We conclude that methionine starvation prevents the formation of the norma Q nucleoside in Rel- E. coli.
mesh_terms: D001224:Aspartic Acid / Q000378:metabolism; D003488:Cyanogen Bromide / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D006639:Histidine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000096:biosynthesis*
chemical_list: D012329:RNA, Bacterial; D006151:Guanosine; D001224:Aspartic Acid; D006639:Histidine; D012343:RNA, Transfer; D008715:Methionine; D007930:Leucine; D003488:Cyanogen Bromide