Release of lipoprotein lipase from fat cells in vitro.
Release of lipoprotein lipase from rat fat cells incubated at 20 degrees in medium with albumin, but without glucose proceeded at a constant rate for 30 min. The initial rate of release was increased when serum was present in the medium. Maximal stimulation (100-300%) was produced with 3.8% serum. The maximal increment in release caused by serum was always greater than that produced by heparin and when both were added release was greater than it was with either one alone. The active component(s) of serum, nondialyzable and stable for 30 min at 56 degrees C, was present in sera from humans and rats in the fed or fasted state. Glucose plus insulin (but neither alone) enhanced the rate of lipase release in the presence of serum but not in its absence. The half-life of the lipase in basal medium of 20 degrees C was 90 min. Heparin decreased this to about 50 min and serum markedly prolonged it whether or not heparin was present. Lipoprotein lipase activity in cells and fractions thereof was assayed in extracts of acetone powders. After centrifugation of fat cell homogenates at 600 times g for 15 min, only 50-60% of the activity was recovered in the supernatant. After centrifugation at 100 000 times g for 60 min, the supernatant contained about 10% of the total activity and the sediment 40%. In some experiments, most of the rest was recovered in the floating fat fraction. Total lipoprotein lipase activity of cells plus medium increased steadily during incubation of fat cells for 1h at 30 degrees C. The major increment occurred in the cells and activity in the medium was always less than 15% of the total. Our observations are consistent with the view that activation may be an important determinant of fat cell lipoprotein lipase activity as well as an integral part of the release process.
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D001769:Blood; D003956:Dialysis; D004822:Epididymis; D005947:Glucose / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006358:Hot Temperature; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D013997:Time Factors
D007328:Insulin; D006493:Heparin; D008071:Lipoprotein Lipase; D005947:Glucose