Thin-layer chromatographic separation of sulfated and nonsulfated lithocholic acids and their glycine and taurine conjugates.
A method for superior thin-layer chromatographic separation of lithocholic acid and its N-glycine and N-taurine conjugates, as well as their respective 3alpha-sulfates, is described. A solvent system of chloroform-methanol-acetic acid-water 65:24:15:9 (v/v) is used with air-dried plates of silicic acid containing calcium sulfate (10% by weight) under conditions of chamber saturation.
D002793:Cholic Acids / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer; D005069:Evaluation Studies as Topic; D005998:Glycine / Q000302:isolation & purification*; D008095:Lithocholic Acid / Q000302:isolation & purification*; D013464:Sulfuric Acids / Q000302:isolation & purification; D013654:Taurine / Q000302:isolation & purification*
D002793:Cholic Acids; D013464:Sulfuric Acids; D013654:Taurine; D008095:Lithocholic Acid; D005998:Glycine