Preparation of 14C-labeled sterigmatocystin in liquid media.
14C-labeled sterigmatocystin was prepared from surface cultures of Aspergillus versicolor A-18074 maintained in liquid media by multiple additions of (1-14C)acetate to the cultures. The highest yield of 7.75 mg/10 ml was found with a sucrose-asparagine-ammonium medium in which more than 3% of the radioactivity of the added (1-14C)acetate was recovered ithe purified (ring-14C) sterigmatocystin. The method offers an easy way to prepare 14C-labeled sterigmatocystin for studies of this mycotoxin.
D000085:Acetates / Q000378:metabolism; D000645:Ammonium Sulfate; D001216:Asparagine; D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D001345:Autoradiography; D002250:Carbon Radioisotopes; D003470:Culture Media; D005663:Furans / Q000096:biosynthesis / Q000302:isolation & purification; D009183:Mycotoxins / Q000096:biosynthesis* / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet; D013395:Sucrose; D014966:Xanthenes / Q000096:biosynthesis* / Q000302:isolation & purification
D000085:Acetates; D002250:Carbon Radioisotopes; D003470:Culture Media; D005663:Furans; D009183:Mycotoxins; D014966:Xanthenes; D013395:Sucrose; D001216:Asparagine; D000645:Ammonium Sulfate