Dissociation of MNSs antigens using sodium deoxycholate fraction of erythrocyte membranes.
A dissociation of the MNSs blood group system has been demonstrated by sodium deoxycholate fractionation of erythrocyte membrane preparations. The MN antigens were localized in a fraction that sedimented at 30,000 g while the Ss antigens remained soluble after 100,000 g centrifugation for donors who were heterozygous at the MN locus. In contrast, fractions derived from erythrocytes of 'homozygous' M or N donors showed M or N activity in both the 30,000 g pellet and 100,000 g supernatant and Ss remained in the 100,000 g supernatant.
D001789:Blood Group Antigens / Q000302:isolation & purification*; D002462:Cell Membrane / Q000276:immunology*; D005591:Chemical Fractionation; D003298:Coombs Test; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004912:Erythrocytes / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007106:Immune Sera; D007519:Isoantigens / Q000032:analysis; D010641:Phenotype; D014461:Ultracentrifugation
D001789:Blood Group Antigens; D007106:Immune Sera; D007519:Isoantigens; D003840:Deoxycholic Acid