Paramyxovirus-avian cell relationship: discrepant impact of 6-azauridine on virus production by susceptible and less susceptible cells.
The replication of mumps virus in susceptible chicken embryonic heart cells was escalated by daily treatment of cultures with 6-azauridine (6-AU). On the other hand, virus production by less susceptible liver cells was depressed by 6-AU. The population of infected susceptible cells was not increased, but the release of virus by infected susceptible cells was enhanced 20-fold by 10 mug of 6-AU per ml. Less susceptible cells, which incorporated less ribonucleic acid and protein precursor than susceptible cells, sustained a constant level of viral release during 6-AU treatment; however, the number of infected less susceptible cells underwent substantial decline.
D000818:Animals; D001380:Azauridine / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002642:Chick Embryo; D046508:Culture Techniques; D008099:Liver; D009109:Mumps virus / Q000187:drug effects; D009206:Myocardium; D010252:Paramyxoviridae / Q000254:growth & development*; D012367:RNA, Viral / Q000096:biosynthesis; D014316:Tritium; D014357:Trypsin; D014529:Uridine / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
D002250:Carbon Radioisotopes; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D001380:Azauridine; D014357:Trypsin; D014529:Uridine