title: Comparative glycolytic metabolism of sperm from normal, asthenospermic, and oligoasthenospermic men.
abstract: The glycolysis of spermatozoa from normal, asthenospermic, and oligoasthen ospermic men was studied using a respirometry technique to measure glucose utilization by the production of 14CO2 from glucose 14C (U-L). Lactate and pyruvate were measured by a spectrophotometric method using DNA as reference. Human spermatozoa preferred glucose to fructose as the glycolytic substrate when concentrations of these hexoses did not exceed normal concentrations in the blood. Spermatozoa from oligoasthenospermic men produced an average of 3.5 times more 14CO2 (345, 457 dpm/mg DNA/hour) than did spermatozoa from asthenospermic (88,837 dpm/mg DNA/hour) and normal men (96,595 dpm/mg DNA/hour). They also formed four times more lactate (9.63 mumoles/mg DNA/hour) than spermatozoa from normal men (2.33 mumoles/mg DNA/hour) and 6.4 times more pyruvate (2.90 mumoles/mg DNA/hour compared to 0.45 mumoles/mg DNA/hour). Spermatozoa from asthenospermic men formed amounts of lactate (3.01 mumoles/mg DNA/hour) and pyruvate (0.63 mumoles/mg DNA/hour) similar to those produced by spermatozoa from normal men.
mesh_terms: D002245:Carbon Dioxide / Q000096:biosynthesis; D002250:Carbon Radioisotopes; D004247:DNA / Q000096:biosynthesis; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D006601:Hexoses / Q000378:metabolism*; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism*; D007315:Insemination, Artificial; D007773:Lactates / Q000096:biosynthesis; D008297:Male; D011773:Pyruvates / Q000096:biosynthesis; D013053:Spectrophotometry; D013094:Spermatozoa / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002250:Carbon Radioisotopes; D006601:Hexoses; D007773:Lactates; D011773:Pyruvates; D002245:Carbon Dioxide; D005632:Fructose; D004247:DNA; D005947:Glucose