Genetic control of specific immune suppression. I. Experimental conditions for the stimulation of suppressor cells by the copolymer L-glutamic acid50-L-tyrosine50 (GT) in nonresponder BALB/c mice.
In the present studies we have confirmed that the random copolymer of L-glutamic acid50-L-tyrosine50 (GT) fails to induce an antibody response in a large number of inbred strains of mice. Nevertheless, GT complexed to methylated bovine serum albumin (MBSA) elicits a GT-specific IgG PFC response in vivo. Furthermore, injection of BALB/c mice with 10 to 100 mug of GT specifically decreases their ability to develop anti-GT PFC responses to a subsequent challenge with GT-MBSA. GT-specific tolerance can be transferred to normal, syngeneic recipients by spleen cells or thymocytes of GT-primed animals. These results indicate that the stimulation of suppressor cells can be observed in nonresponder mice with another synthetic polypeptide besides GAT. Various parameters of GT-specific immunosuppression in BALB/c mice are described. The application of these techniques to the study of the genetic factors controlling the stimulation of specific immune suppression is discussed.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D005971:Glutamates / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007108:Immune Tolerance; D007114:Immunization; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D011108:Polymers / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D014443:Tyrosine / Q000276:immunology
D000941:Antigens; D005971:Glutamates; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D011108:Polymers; D012710:Serum Albumin, Bovine; D014443:Tyrosine