title: Action of the mycotoxin of Fusarium sporotrichiella v. sporotrichioides on lysosomal membranes.
abstract: The effect of the mycotoxin of Fusarium sporotrichiella v. sporotrichioides (sporofusarin) was studied in vitro on the total and nonsedimenting activity of eight lysosomal enzymes: acid ribonuclease, aryl sulfatases A and B, beta-glucuronidase, alpha- and beta-galactosidases, beta-glucosidase, beta-acetylglucosaminidase, and alpha-mannosidase. Incubation of a suspension of rat liver lysosomes with an aqueous solution of sporofusarin led to inhibition of the total activity of the membrane-bound lysosomal enzyme beta-glucosidase. In a dose of only 1.6 x 10-5 M sporofusarin caused a significant increase in the nonsedimenting activity of nearly all the enzymes; in a concentration of 1.6 x 10-3 M most of the enzymes of the lysosomal matrix (beta-glucuronidase, beta-galactosidase, aryl sulfatases A and B) were liberated almost completely into the supernatant, and nearly all the beta-glucosidase also was liberated. It is postulated that damage to the subcellular membranes is an important component of the toxic action of sporofusarin.
mesh_terms: D000818:Animals; D005670:Fusarium; D005959:Glucosidases / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism*; D008099:Liver / Q000648:ultrastructure; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008358:Mannose; D008566:Membranes / Q000187:drug effects; D009183:Mycotoxins / Q000494:pharmacology*; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D013429:Sulfatases / Q000378:metabolism
chemical_list: D009183:Mycotoxins; D006867:Hydrolases; D012260:Ribonucleases; D013429:Sulfatases; D005959:Glucosidases; D006026:Glycoside Hydrolases; D005966:Glucuronidase; D008358:Mannose