The biology of isolated immunocytes. I. Isolation into a closed liquid microchamber: application to PFC.
Biological properties of isolated immunocytes directly involved in an immune response such as plaque forming cells may be studied in a closed liquid microchamber. Furthermore the selected cells can be cultured for over three days; immunocytological treatments of these cells permit also their characterization by electron microscopy.
D000818:Animals; D000921:Antibody-Producing Cells; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007104:Immune Adherence Reaction; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008846:Micromanipulation; D011817:Rabbits / Q000276:immunology; D012756:Sheep; D013154:Spleen / Q000166:cytology