Submicrogram assay for scopolamine in plasma and urine.
A GLC-mass spectrometric method for scopolamine, sensitive to 50 pg/ml for a 4-ml plasma or urine sample, was developed. The method used a deuterated internal standard to minimize variability in absolute recovery in the extraction procedure. Scopoline and deuterated scopoline were formed from the base-catalyzed hydrolysis of scopolamine and the internal standard and were analyzed as the heptafluorobutyrates, using a GLC-mass spectrometric system by monitoring the m/e 138 and 141 fragments, respectively.
D002849:Chromatography, Gas; D003903:Deuterium; D006801:Humans; D013058:Mass Spectrometry; D008722:Methods; D008832:Microchemistry; D012601:Scopolamine / Q000032:analysis* / Q000097:blood / Q000652:urine
D003903:Deuterium; D012601:Scopolamine