The behaviour of LDH-3 in patients with malignant diseases during therapy with cytostatic drugs and prednisone, studied by LDH-isoenzyme electrophoresis on cellulose acetate.
A modified method used for the quantitative estimation of LDH-isoenzymes in serum after electrophoresis on cellulose acetate is described. Total LDH-activity and isoenzyme distribution in serum samples of capillary blood are compared to those in samples collected by venipuncture. Total LDH-values and LDH-distributions both in 29 normal children and 14 children with malignant diseases are given. When studying the LDH-distribution in serum samples of children with malignant diseases a significantly decreased LDH-3 was found in many patients. LDH-4 and LDH-5 were also reduced. It was noted that these children all received therapy with prednisone. Possible explanations are discussed.
D000217:Acyltransferases / Q000097:blood; D000410:Alanine Transaminase / Q000097:blood; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002648:Child; D004586:Electrophoresis; D005971:Glutamates; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D009369:Neoplasms / Q000188:drug therapy / Q000201:enzymology*; D011241:Prednisone / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D005971:Glutamates; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D000217:Acyltransferases; D000410:Alanine Transaminase; D011241:Prednisone