title: Stimulation of the uptake of soluble proteins into isolated HeLa nuclei by pancreatic deoxyribonuclease.
abstract: The uptake into isolated HeLa nuclei of radioactive cytosol proteins and purified Escherichia coli ribosomal protein L7 is stimulated up to 4-fold by pancreatic deoxyribonuclease (DNase I). Similar effects are not observed with pancreatic ribonuclease A or phospholipase C. The results reported suggest that there is a general stimulatory effect of DNase on protein uptake by nuclei.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000494:pharmacology*; D004996:Ethidium / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D010740:Phospholipases / Q000494:pharmacology; D012260:Ribonucleases / Q000494:pharmacology; D012270:Ribosomes / Q000378:metabolism; D014316:Tritium
chemical_list: D001426:Bacterial Proteins; D009363:Neoplasm Proteins; D014316:Tritium; D003609:Dactinomycin; D003851:Deoxyribonucleases; D010740:Phospholipases; D012260:Ribonucleases; D004996:Ethidium; D007930:Leucine