Genetic studies on Plasmodium chabaudi: recombination between enzyme markers.
Two lines of Plasmodium chabaudi differing in three characters have been crossed, using a technique previously described for P. yoelii. One line, termed 47AS, was characterized by an electrophoretic form of 6-hosphogluconate dehydrogenase, denoted 6PGD-2, a form of lactate dehydrogenase, denoted LDH-3, and was pyrimethanime-resistant. The second line, termed 10AJ, possessed enzyme forms of 6PGD-3 and LDH-2 and was pyrimethamine-sensitive. The cross was made by permitting mosquitoes to feed on a mixture of the two lines and infecting rodents with the resulting sporozoites. The products of the cross were cloned by dilution and examined for enzyme-type and drug-response. Results showed that recombination had occurred between each of the three characters. Clones characterized by 6PGD-2/LDH-2 and 6PGD-3/LDH-3 demonstrated recombination between the enzyme markers. The drug-resistance character segregated independently of either enzyme marker.
D000818:Animals; D003433:Crosses, Genetic; D004352:Drug Resistance, Microbial; D005796:Genes; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis; D010961:Plasmodium / Q000187:drug effects / Q000201:enzymology; D011739:Pyrimethamine / Q000494:pharmacology; D011995:Recombination, Genetic
D007770:L-Lactate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D011739:Pyrimethamine