Biochemical aspects of the visual process.  XXVII. Stereospecificity of ocular retinol dehydrogenases and the visual cycle.
A comparative study is made of the stereospecificity of two particulate retinol dehydrogenases from bovine eyes and of horse liver alcohol dehydrogenase.  The particulate retinol dehydrogenase of outer segments reacts with the all-trans isomers of retinaldehyde and retinol but not with the 11-cis  compounds.  In contrast, a particulate retinol dehydrogenase present in pigment epithelium reacts preferentially with the 11-cis compounds.  Horse liver alcohol dehydrogenase (EC 1.1.1.1.) can convert both isomers, but the all-trans isomers are clearly preferred.  Differences with regard to cofactor preference and stability are also noted.  The outer segment enzyme clearly functions in the rhodopsin cycle.  It is unlikely that the 11-cis retinol dehydrogenase from pigment epithelium is directly involved in providing 11-cis retinaldehyde from rhodopsin regeneration, but it may serve to make available 11-cis retinaldehyde from rhodopdsin, digested in phagocytized rod sacs, for the synthesis of visual pigment by the visual cells.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003902:Detergents / Q000494:pharmacology; D004072:Digitonin / Q000494:pharmacology; D004848:Epithelium / Q000201:enzymology; D006736:Horses; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008954:Models, Biological; D009928:Organ Specificity; D010786:Photoreceptor Cells / Q000187:drug effects / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D013045:Species Specificity; D013237:Stereoisomerism; D014785:Vision, Ocular; D014801:Vitamin A
D003902:Detergents; D014801:Vitamin A; D011092:Polyethylene Glycols; D000429:Alcohol Oxidoreductases; D004072:Digitonin