Binding of ethidium bromide to ribosomal RNA. Absorption, fluorescence, circular and electric dichroism study.
The interaction between ethidium bromide and ribosomal RNA has been studied by means of absorption, fluorescence, circular and electric dichroism measurements in the near ultraviolet and visible regions at low ionic strength (1 . 10(-3) and 6 . 10(-3). The results have been interpreted on the basis of a model of interaction involving the intercalation of the phenanthridinium ring of the dye in the double-stranded regions of the RNA molecule, resulting in an increase of the dye-dye interactions as compared to DNA, and a stiffening of the intercalation regions.
D001665:Binding Sites; D002942:Circular Dichroism; D004996:Ethidium; D007700:Kinetics; D008433:Mathematics; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal; D012441:Saccharomyces cerevisiae; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013057:Spectrum Analysis
D012335:RNA, Ribosomal; D004996:Ethidium