Sugar transport by renal plasma membrane vesicles. Characterization of the systems in the brush-border microvilli and basal-lateral plasma membranes.
Uptake studies of D-and L-glucose were performed on vesicles derived from brush-border and basal-lateral membranes. The uptake of the sugars into the vesicles was osmotically sensitive and independent of glucose metabolism. In brush-border vesicles D-glucose but not L-glucose transport was Na-+-dependent, wn the presence of an initial Na+gradient. Basal-lateral membranes take up D-glucose faster than L-glucose, but the D-glucose uptake is significantly less sensitive to sodium removal and only moderately inhibited by phlorzin as compared to the prush-border fraction.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism*; D002642:Chick Embryo; D005947:Glucose / Q000378:metabolism*; D007672:Kidney Cortex / Q000378:metabolism*; D009994:Osmolar Concentration; D010693:Phloretin / Q000494:pharmacology; D010695:Phlorhizin / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D013237:Stereoisomerism; D013696:Temperature
D012964:Sodium; D010695:Phlorhizin; D005947:Glucose; D010693:Phloretin