Fatty acid metabolism in the microsomal fraction of developing rabbit brain.
1. Incorporation of [14C]malonyl-CoA into fatty acid and lipid by the microsomal fraction from developing rabbit brain has been determined. 2. The specific activity for malonyl-CoA incorporation into preformed fatty acid reached a maximum at 15-20 days of age and the increase in enzyme activity paralleled that of palmitoyl-CoA synthesis in the microsomal fraction. 3. Most of the preformed fatty acid was derived from microsomal membrane lipid, and added acyl-CoA only slightly increased the incorporation. Inhibition occurred at concentrations in excess of 2 muM acyl-CoA. Endogenous acyl-CoA may be formed by acyl-group turnover through the action of phospholipase A and acyl-CoA synthetase. 4. Added 14C-labelled acyl-CoA was elongated to a longer chain-length product than endogenous fatty acid elongated with [14C]malonyl-CoA. 5. Both ATP and acyl-CoA influence the incorporation of elongated fatty acids into complex lipids, possibly through their effect on acyl-CoA synthetase.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D003065:Coenzyme A / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D008314:Malonates / Q000378:metabolism; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D010169:Palmitic Acids / Q000378:metabolism; D011817:Rabbits; D013329:Structure-Activity Relationship; D013997:Time Factors
D005227:Fatty Acids; D008314:Malonates; D010169:Palmitic Acids; D000255:Adenosine Triphosphate; D003065:Coenzyme A