[Production of the methyl ether of 8,14-seco-delta,3,5,(10),9(11)-estratetraendiol-3,17beta-one-14 using a culture of Saccharomyces cerevisiae BKMU-488].
The culture Saccharomyces cerevisiae BKMU-499 was selected from 100 strains of different Saccharomyces species to obtain an optically active methyl ester 8,14-seco-delta1,3,5(10),9(11)-estratetraendiol-3-17beta-on-14. Various conditions of the transformation were examined. The yield of this keto-alcohol depended on the following factors: level of aeration, method of steroid administration, physiological state of the culture, and composition of the cultivation medium. The optimal yeild of the optically active product was 84%.
D000332:Aerobiosis; D003470:Culture Media; D004963:Estrenes / Q000096:biosynthesis*; D007659:Ketones / Q000378:metabolism; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012632:Secosteroids / Q000096:biosynthesis*; D013997:Time Factors
D003470:Culture Media; D004963:Estrenes; D007659:Ketones; D012632:Secosteroids
Poluchenie metilovogo éfira 8,14-seco-del'tal, 3,5(10),9(11)-éstratetradiola-3,17beta-ona-14 s pomoshch'iu kul'tury Saccharomyces cerevisiae BKMU-488