title: On the enthalpy of binding of ADP to heavy meromyosin.
abstract: The binding of ADP to heavy meromyosin has been studied by microcalorimetry. Minute amounts of myokinase interfere with binding measurements, but by selection of appropriate conditions, we can estimate that the value of the apparent deltaHbinding lies between - 1.0 and - 3.0 kcal per mole of ADP bound (0.3 M KC1, 2 mM MgC12, 20mM Tris, pH 8.00, 20 degrees C). Values of deltaHbinding reported to date are an order of magnitude larger, and we suggest that these values are artifactual results due to myokinase contamination.
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D004734:Energy Metabolism; D015879:Myosin Subfragments / Q000378:metabolism*
chemical_list: D015879:Myosin Subfragments; D000244:Adenosine Diphosphate