XLV. Metabolism of 3beta,7alpha-dihydroxy-5alpha-cholestanoic acid in the rat with a bile fistula.
[25R]3beta,7alpha-Dihydroxy-[5alpha,6alpha- -3 H2]-5alpha-cholestanoic acid was prepared, purified and 0.34 mg was administered intraperitoneally as the potassium salt to each of three adult male rats with cannulated bile ducts. Bile collected in the first 24 h, containing 97% of the administered 3-H was hydrolysed and the free bile acids were separated by acetic acid partition chromatography. Of the chromatographed tritium 58% was associated with allochenodeoxycholic acid and 14% with its 3beta-isomer; only 5% of the 3-H was found in allocholic acid and 1% with the substrate or more polar unidentified materials. Thus, this dihydroxy-5alpha-cholestanoic acid is metabolized in the rat primarily to dihydroxy-5alpha-cholanic acids, comparable to the metabolism of 3alpha, 7alpha-dihydroxy-5beta-cholestanoic acid in man.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism; D001652:Bile Ducts; D002404:Catheterization; D002635:Chenodeoxycholic Acid / Q000096:biosynthesis; D002776:Cholestanes / Q000378:metabolism*; D002845:Chromatography; D002855:Chromatography, Thin Layer; D051381:Rats; D013997:Time Factors
D001647:Bile Acids and Salts; D002776:Cholestanes; D002635:Chenodeoxycholic Acid