Improved rapid method to check electrode localizations in the brain.
A modification of known rapid methods to check electrode localization in the brain is described: the essential point consists in immersing the brain, after fixation in 4 percent Formalin, in pure heptane at -30 degrees C for 5 hr. Unstained sections, cut with the freezing microtome, are placed uncovered on glass slides and photographed (in an enlarger, as if they were negatives) either wet, to obtain  a contrast resembling Weil staining, or dry, to obtain a picture corresponding to Nissl staining.
D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D002415:Cats; D005615:Freezing; D006168:Guinea Pigs; D006652:Histological Techniques; D051381:Rats; D013238:Stereotaxic Techniques