title: Micromethod for lead determination in whole blood by atomic absorption, with use of the graphite furnace.
abstract: I describe a micro-scale method for determining lead in whole blood by utilizing a graphite furnace.  Sample pretreatment consists of fivefold dilution with a dilute surfactant.  The method is directly calibrated with lead standards prepared in dilute HNO3.  To eliminate a small, nonspecific absorption signal from the blood matrix, simultaneous background correction is used.  Interlaboratory comparison with a flame atomic absorption technique that requires extraction yielded high correlation (r equal to 0.98).  Within-run precision (coefficient of variation) ranged from 2 to 4 percent.  Lead in blood can be accurately measured in as little as 20 mul of blood, hence the method is suitable for routine laboratory use and for pediatric screening.
mesh_terms: D003903:Deuterium; D005069:Evaluation Studies as Topic; D006108:Graphite; D006358:Hot Temperature; D006801:Humans; D007854:Lead / Q000097:blood*; D008722:Methods; D008832:Microchemistry; D013054:Spectrophotometry, Atomic; D013997:Time Factors
chemical_list: D007854:Lead; D006108:Graphite; D003903:Deuterium