Improved method of selection for mutants of Pseudomonas putida.
Optimum conditions for enrichment of mutants of Pseudomonas putida in liquid culture were established using a procedure which combines N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis with an improved D-cycloserine selection.
D001431:Bacteriological Techniques; D003470:Culture Media; D003523:Cycloserine; D006639:Histidine / Q000378:metabolism; D008769:Methylnitronitrosoguanidine; D009153:Mutagens; D009154:Mutation; D011549:Pseudomonas / Q000302:isolation & purification* / Q000378:metabolism
D003470:Culture Media; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D006639:Histidine; D003523:Cycloserine