Mechanism of inhbition of protein systhesis initiation by diacetoxyscirpenol and fusarenon X in the reticulocyte lysate system.
The mechanism of inhibition of peptide chain initiation by diacetoxyscirpenol and fusarenon X, (trichotecene mycotoxins), was studied in the reticulocyte lysate protein-synthesizing system. The mycotoxins did not inhibit formation of the complex between Met-tRNA-f and the 40S ribosomal subunit nor the initiation codon AUG-promoted combination of the complex with  60S ribosomal subunit. Formyl-methionyl-valine accumulated when the system was incubated with the mycotoxins and fMet-tRNA-f. This suggests that the inhibition occurs after the formation of the first peptide bond.
D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis*; D002499:Centrifugation, Density Gradient; D007930:Leucine / Q000378:metabolism; D008715:Methionine; D009183:Mycotoxins / Q000494:pharmacology*; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects*; D012343:RNA, Transfer / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D012717:Sesquiterpenes / Q000494:pharmacology*; D013997:Time Factors; D014255:Trichothecenes; D014633:Valine
D001798:Blood Proteins; D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes; D012343:RNA, Transfer; D008715:Methionine; D007930:Leucine; D014633:Valine; C001809:diacetoxyscirpenol