title: Mechanism of the mutagenic action of hydroxylamine. VIII. Functional properties of the modified adenosine residues.
abstract: The action of O-methylhydroxylamine in vitro upon some amber and rII ochre mutants of T4 phage leads to a considerable increase in the frequency of reversions and conversions of ochre and opal mutants presumably due to A yields G transitions. The transitions seem to be caused by modification of the adenine bases  of the genome to give N-6-methoxyadenine which has equivocal (A- and G-like) specificity. However, the functional activity of N-6-methoxyadenosine residues in the RNA polymerase system is low. We failed to detect any significant activity of these residues either as components of a template polyribonucleotide, or as nucleoside triphosphate precursors.
mesh_terms: D000227:Adenine Nucleotides / Q000138:chemical synthesis; D000241:Adenosine / Q000378:metabolism*; D003090:Coliphages / Q000187:drug effects / Q000378:metabolism*; D004267:DNA Viruses / Q000187:drug effects; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D006898:Hydroxylamines / Q000494:pharmacology*; D007700:Kinetics; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D013698:Templates, Genetic; D013997:Time Factors
chemical_list: D000227:Adenine Nucleotides; D006898:Hydroxylamines; D009153:Mutagens; D012321:DNA-Directed RNA Polymerases; D000241:Adenosine