Antibody response by cells cultured on dialysis membrane in disposable plastic vessels.
Plastic vessels were used to culture mouse spleen cells by the technique of Marbrook (1967) and good primary antibody responses were obtained. These vessels some advantages over glassware, which may allow a wider use of this technique.
D000818:Animals; D000917:Antibody Formation; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D008214:Lymphocytes / Q000276:immunology*; D008567:Membranes, Artificial; D051379:Mice; D010969:Plastics; D013154:Spleen / Q000166:cytology
D008567:Membranes, Artificial; D010969:Plastics