Lomofungin inhibition of allophanate hydrolase synthesis in Saccharomyces cerevisiae.
The RNA polymerase inhibitor, lomofungin has been used to determine the half life of specific synthetic capacities (invertase and alpha-glucosidase) as well as that for gross protein synthesis. In both cases the studies conclude that cognate messenger RNAs decay with a half life of approximately 20 minutes. This antibiotic has been used to determine the half life of allophanate hydrolase specific synthetic capacity. We find that it decays with a half life of about three minutes; a value that agrees with the decay rates of allophanate hydrolase synthetic capacity following removal of inducer. These observations argue that mRNA may be metabolized by two separate routes in Saccharomyces.
D000492:Allophanate Hydrolase / Q000096:biosynthesis*; D000581:Amidohydrolases / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000096:biosynthesis; D006207:Half-Life; D007930:Leucine / Q000378:metabolism; D010619:Phenazines / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014498:Uracil / Q000378:metabolism
D005656:Fungal Proteins; D010619:Phenazines; D014498:Uracil; D012313:RNA; D000581:Amidohydrolases; D000492:Allophanate Hydrolase; D007930:Leucine