title: Determinants of glomerular filtration in experimental glomerulonephritis in the rat.
abstract: Pressures and flows were measured in surface glomerular capillaries, efferent arterioles, and proximal tubules of 22 Wistar rats in the early autologous phase of nephrotoxic serum nephritis (NSN). Linear deposits of rabbit and rat IgG and C3 component of complement were demonstrated in glomerular capillary walls by immunofluorescence microscopy. Light microscopy revealed diffuse proliferative glomerulonephritis, and proteinuria was present. Although whole kidney and single nephron glomerular filtration rate (GFR) in NSN (0.8 plus or minus 0.04 SE2 ml/min and 2 plus or minus 2 nl/min, respectively) remained unchanged from values in 16 weight-matched NORMAL HYDROPENIC control rats (0.8 plus or minus 0.08 and 28 plus or minus 2), important alterations in glomerular dynamics were noted. Mean transcapillary hydraulic pressure difference (deltaP) averaged 41 plus or minus 1 mm Hg in NSN versus 32 plus or minus 1 in controls (P LESS THAN 0.005). Oncotic pressures at the afferent (piA) end of the glomerular capillary were similar in both groups ( 16 mm /g) but increased much less by the efferent end (piE) in NSN (to 29 plus or minus 1 mm Hg) than in controls (33 plus or minus 1, P less than 0.025). Hence, equality between deltaP and piE, denoting filtration pressure equilibrium, obtained in control but not in NSN rats. While glomerular plasma flow rate was slightly higher in NSN (88 plus or minus 8 nl/min) than in controls (76 plus or minus 6, P greater than 0.2), the failure to achieve filtration equilibrium in NSN rats was primarily the consequence of a marked fall in the glomerular capillary ultrafiltration coefficient, Kf, to a mean value of  0.03 nl/(s times mm Hg), considerably lower than that found recently for the normal rat, 0.08 nl/(s times mm Hg). Thus, despite extensive glomerular injury, evidenced morphologically and by the low Kf, GFR remained normal. This maintenance of GFR resulted primarily from increases in deltaP, which tended to increase the net driving force for filtration, and thereby compensate for the reduction in Kf.
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D002196:Capillaries / Q000276:immunology / Q000503:physiopathology / Q000648:ultrastructure; D003165:Complement System Proteins; D005919:Glomerular Filtration Rate; D005921:Glomerulonephritis / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000302:isolation & purification; D007444:Inulin / Q000097:blood / Q000378:metabolism; D007668:Kidney; D007672:Kidney Cortex / Q000276:immunology / Q000473:pathology; D007678:Kidney Glomerulus / Q000098:blood supply / Q000503:physiopathology / Q000648:ultrastructure; D007687:Kidney Tubules, Proximal / Q000503:physiopathology; D007962:Leukocytes / Q000648:ultrastructure; D008433:Mathematics; D011507:Proteinuria / Q000276:immunology; D051381:Rats
chemical_list: D007106:Immune Sera; D007074:Immunoglobulin G; D007444:Inulin; D003165:Complement System Proteins