title: Separation of pineal extracts by gelfiltration. II. Identification and isolation of two indoles from sheep pineal glands.
abstract: After separation of aqueous extracts of sheep pineal bodies and sheep cerebral cortex on Sephadex G-25, a high fluorescence peak showing an excitation maximum at 305 leads to 310 nm and a fluorescence maximum at 350 leads to 355 nm was observed in pineal extracts, which could not be detected in cerebral cortex extracts. Separation of this pineal fluorescence peak on Sephadix G-10 with water elution, followed by gradient elution of water and NaOH, gave rise to several highly fluorescent peaks. From one of these it was possible to isolate a substance with Rf-values, using thinlayer chromatographic, fluorescence and mass spectra studies, identical with synthetic 5-hydroxyindole-3-acetic acid. From another peak a substance, idential with synthetic 5-methoxyindole-3-acetic acid, could be isolated. The results are compared with those obtained in previous studies.
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000032:analysis; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004590:Electrophoresis, Paper; D006897:Hydroxyindoleacetic Acid / Q000302:isolation & purification; D007210:Indoleacetic Acids / Q000031:analogs & derivatives / Q000302:isolation & purification*; D013058:Mass Spectrometry; D010870:Pineal Gland / Q000032:analysis*; D012756:Sheep; D013050:Spectrometry, Fluorescence; D014020:Tissue Extracts
chemical_list: D007210:Indoleacetic Acids; D014020:Tissue Extracts; D006897:Hydroxyindoleacetic Acid