In situ kidney preservation for transplantation with use of profound hypothermia (5 to 20 degrees C.) with an intact circulation.
Twenty-seven anesthetized dogs were surface cooled at 4 to 6 degrees C. or 15 to 20 degrees C. Circulation was provided by the mechanical ventricular assist in 23 dogs. After 24 or 48 hours of in situ preservation, the kidneys were transplanted into the necks or iliac fossas of anephric recipients. Renal function was preserved in the cadaver for up to 48 hours at 15 to 20 degrees C. by maintaining a pulsatile circulation. Further cooling to 4 to 6 degrees C. caused progressive deterioration in renal function. The nonperfused kidneys kept in situ at 4 to 6 degrees C. did not produce any urine after transplantation. If the practical problems of total body cooling are solved, in situ preservation of multiple organs in the cadaver would increase the number of available organs for transplantation.
D000818:Animals; D001775:Blood Circulation; D001794:Blood Pressure; D004285:Dogs; D005112:Extracorporeal Circulation; D007036:Hypothermia, Induced; D007668:Kidney / Q000098:blood supply / Q000503:physiopathology; D016030:Kidney Transplantation; D014021:Tissue Preservation; D014184:Transplantation, Homologous