Readenylation of polyadenylate-free globin messenger RNA restores its stability in vivo.
Using an ATP:RNA adenyltransferase from Escherichia coli, a polyadenylic sequence was resynthesized onto rabbit globin mRNA from which the poly (A) segment had been previously removed. Conditions for obtaining a homogenous reconstituted globin mRNA preparation containing 30 adenylic residues per message molecule were determined. The reconstituted globin mRNA was microinjected into Xenopus laevis oocytes. Its stability was very similar to that of native mRNA.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D004355:Drug Stability; D004926:Escherichia coli / Q000201:enzymology; D005260:Female; D005914:Globins / Q000096:biosynthesis*; D006454:Hemoglobins / Q000096:biosynthesis; D009865:Oocytes / Q000378:metabolism; D011061:Poly A; D014176:Protein Biosynthesis; D012316:RNA Nucleotidyltransferases; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D014981:Xenopus
D000227:Adenine Nucleotides; D006454:Hemoglobins; D012333:RNA, Messenger; D011061:Poly A; D005914:Globins; D012316:RNA Nucleotidyltransferases