Adrenal and liver metabolites of 18-hydroxy-11-deoxycorticosterone in the rat. Identification of reduced compounds (18-OH-TH-DOC).
The presence of reduced metabolites of 18-hydroxy-11-deoxycorticosterone has been investigated in the adrenals of 23 day-old and adult rats and in the liver of adult rats. By thin-layer chromatography a fraction of the adrenal steroid extract migrating like tetrahydrocorticosterone has been isolated. By gas chromatography-mass spectrometry several isomers of 3,18,21-trihydroxy-pregnan-20-one (18-OH-TH-DOC) have been separated in this fraction and identified by comparison with authentic samples which have been chemically and enzymatically synthesized. The major tetrahydrogenated metabolite in the adult and prepuberal rat adrenals is 3beta,18,21-trihydroxy-5alpha-pregnan-20-one (18-OH-TH-DOC II). The 3alpha,18,21-trihydroxy-5beta-pregnan-20-one has been found only in the prepuberal rat adrenal. A third tetrahydrogenated isomer has been tentatively indentified as 3alpha,18,21-trihydroxy-5alpha-pregnan-20-one. Quantitative measurements by mass fragmentography show that adrenal reductase activity on 18-hydroxy-11-deoxycorticosterone is higher than on corticosterone. The 18-OH-TH-DOC II has been identified in the liver of adult male rat.
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002849:Chromatography, Gas; D003345:Corticosterone / Q000378:metabolism; D003900:Desoxycorticosterone / Q000031:analogs & derivatives* / Q000378:metabolism; D006889:Hydroxycorticosteroids / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D013058:Mass Spectrometry; D009928:Organ Specificity; D010084:Oxidation-Reduction; D051381:Rats
D006889:Hydroxycorticosteroids; D003900:Desoxycorticosterone; D003345:Corticosterone