Increased urinary excretion of labelled deoxyribonucleosides in rats with stable pre-labelled DNA treated with methyl methanesulphonate or nitrogen mustard.
After the DNA of newborn female rats had been labelled by repeated injections of [14C]orotate (totalling 36 mu Ci) during the first 3 weeks of life, approximately 1,000,000 dpm were found in the DNA of the liver, lungs, kidneys, gut, brain, heart and spleen of 8-week-old rats. Methyl methanesulphonate (MMS) (80 mg/kg) and di-(2-chloroethyl)methylamine (HN2) (5 mg/kg) injection increased the amount of 14C-labelled DNA pyrimidine nucleosides excreted in the urine to 5000 dpm from 350 dpm before injection. The effect on RNA products was much less marked.
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D002846:Chromatography, Affinity; D004247:DNA / Q000378:metabolism; D003853:Deoxyribonucleosides / Q000652:urine; D005260:Female; D007279:Injections, Subcutaneous; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008466:Mechlorethamine / Q000008:administration & dosage / Q000494:pharmacology*; D008741:Methyl Methanesulfonate / Q000008:administration & dosage / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism; D009963:Orotic Acid / Q000378:metabolism; D011741:Pyrimidine Nucleosides / Q000378:metabolism / Q000652:urine; D051381:Rats; D013154:Spleen / Q000378:metabolism
D002250:Carbon Radioisotopes; D003853:Deoxyribonucleosides; D011741:Pyrimidine Nucleosides; D008466:Mechlorethamine; D009963:Orotic Acid; D004247:DNA; D008741:Methyl Methanesulfonate