Determination of coumaphos and its oxygen analog in eggs by in situ fluorometry.
A method is reported for the simultaneous determination of coumaphos (o,o-diethyl o(3-chloro-4-methyl-2-oxo-2H-1-benzopyran-7-yl)phosphorothioate) and its oxygen analog, Coroxon, in eggs in the ppb range. The residues are extracted with acetone and chloroform. The extract is purified by liquid-liquid partition followed by column chromatography and then by 2-dimensional thin layer chromatography. The fluorescence is measured directly on the chromatogram.
D000818:Animals; D002855:Chromatography, Thin Layer; D003372:Coumaphos / Q000031:analogs & derivatives / Q000032:analysis*; D003374:Coumarins / Q000032:analysis; D004531:Eggs / Q000032:analysis*; D005470:Fluorometry; D007306:Insecticides / Q000032:analysis*; D008722:Methods; D010573:Pesticide Residues / Q000032:analysis*; D012997:Solvents
D003374:Coumarins; D007306:Insecticides; D010573:Pesticide Residues; D012997:Solvents; C007130:coroxon; D003372:Coumaphos