[Isolation and properties of cytoplasmic 21S and 7.5S RNP-particles from rat M-1 sarcoma cells].
RNP-particles, containing components with coefficient of sedimentation 119 S, 78 S, 60 S, 40 S, 21 S and 7.5 S were prepared from cells of sarcoma M-1. After separation in sucrose gradient, physico-chemical properties of 21 S and 7.5 S particles were studied. RNA of the particles was shown to belong to the GC-type. More than 10 protein fractions were found in polyacrylamide gel electrophoresis of these particles in presence of sodium dodecyl sulphate. Some of the proteins possessed electrophoretic mobility similar to that ribosomal proteins. In CsCl2 floating density of 21 S and 7.5 S particles was equal to 1.355 g/cm-3. By means of immunodiffusion method it was shown that light RNP-particles were analogous to the ribosomes of rat liver tissue and sarcoma M-1. Incorporation of 14-C-leucine into protein and 32-P-ortophosphate into RNA within 4, 7 and 12 hrs after administration of the isotopes demonstrated metabolic stability of RNA and high activity of the protein in the RNP-particles studied.
D000362:Agar; D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000166:cytology; D009363:Neoplasm Proteins / Q000032:analysis / Q000302:isolation & purification*; D009698:Nucleoproteins / Q000032:analysis / Q000302:isolation & purification*; D010710:Phosphates / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis / Q000302:isolation & purification*; D051381:Rats; D012265:Ribonucleotides / Q000032:analysis; D012270:Ribosomes / Q000032:analysis; D012513:Sarcoma, Experimental / Q000032:analysis* / Q000473:pathology; D014461:Ultracentrifugation
D009363:Neoplasm Proteins; D009698:Nucleoproteins; D010710:Phosphates; D012334:RNA, Neoplasm; D012265:Ribonucleotides; D000362:Agar; D007930:Leucine
Vydelenie i svoĭstva tsitoplazmaticheskikh 21 S i 7,5 S RNP-chastits iz kletok sarkomy M-1 krys