Effects of 60 and 80% oxygen on cell division in lung alveoli of squirrel monkeys.
The purpose of this study was to determine the effects on cell division of 60 and 80% oxygen in the lung alveoli of squirrel monkeys. To accomplish this, squirrel monkeys were exposed to 60 and 80% oxygen for up to 8 d. Prior to sacrifice, cells preparing to divide were labeled with tritiated thymidine (3H-TdR). Labeled cells were visualized with autoradiographic techinques, counted with the light microscope, and expressed in terms of a labeling index. In the present study, it was shown that DNA synthesis was not changed by exposure to 60%; however, with exposure to 80% oxygen, cell labeling was well above control levels by 5 d. Analysis of the cell types involved showed an increase in labeling. due to an increase in dividing Type 2 cells. Other cells in the alveolar wall also showed an increase in labeling
D000818:Animals; D001273:Atmosphere Exposure Chambers; D001345:Autoradiography; D002455:Cell Division / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D008297:Male; D010100:Oxygen / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000187:drug effects; D012453:Saimiri; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D004247:DNA; D010100:Oxygen; D013936:Thymidine