Rapid determination of (carbon-14) glucose specific radioactivity for in vivo glucose kinetics.
Specific radioactivity of blood glucose was determined by use of a Dowex-1 anion-exchange column after (carbon-14) glucose was infused for in vivo kinetics. The first 20-ml eluate of protein-free blood filtrate from the column was discarded; then 10 ml was collected, lyophilized, and counted in a liquid-scintillation counter. Glucose concentration was determined and specific radioactivity calculated. Kinetic results were comparable to those obtained with the more laborious glucose-pentaacetate procedure.
D000085:Acetates; D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D002250:Carbon Radioisotopes / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D005069:Evaluation Studies as Topic; D005947:Glucose / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics
D000085:Acetates; D001786:Blood Glucose; D002250:Carbon Radioisotopes; D005947:Glucose