Cell growth and thymidine incorporation changes induced by Dolichos lectin in embryo fibroblasts at various stages of differentiation.
We report here the effect of Dolichos lectin on chick embryo fibroblasts from embryos between 6th and 16th day of development. There is evidence that Dolichos lectin decreases cell number and proportion of cells incorporating tritium labelled thymidine in case of chick embryo fibroblasts of 6th, 8th and 10th day of development. Dolichos lectin stimulated the proliferation of 16-day old embryo cells. No effect was noticed on 12-day embryo cells at different concentrations of Dolichos lectin used. This lectin is specifically inhibited by N-acetyl-D-galactosamine and anti-Dolichos lectin serum. The difference in response by cells during different stages of embryonic development could perhaps be explained as some regulatory changes occurring on the cell surface.
D000116:Acetylgalactosamine / Q000494:pharmacology; D000367:Age Factors; D000818:Animals; D001345:Autoradiography; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D002642:Chick Embryo; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts; D037102:Lectins / Q000276:immunology / Q000494:pharmacology* / Q000633:toxicity; D037121:Plant Lectins; D010944:Plants; D013268:Stimulation, Chemical; D013936:Thymidine / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium
D037102:Lectins; D037121:Plant Lectins; D014316:Tritium; D004247:DNA; D000116:Acetylgalactosamine; D013936:Thymidine