Isolation of a DNA fraction highly enriched in transfer RNA genes from Xenopus laevis.
A DNA fraction highly enriched in tRNA genes can be isolated from the Xenopus laevis genome by the use of Ag+/Cs2SO4 density gradients. Ag+ shows a low affinity for some tRNA cistrons, allowing their separation from bulk DNA upon equilibrium centrifugation in a Cs2SO4 density gradient. Contaminating DNA in the resulting tDNA fraction is further removed by two additional CsCl density gradient centrifugations. The final DNA fraction is 60-fold enriched in tRNA genes, compared to the starting DNA material.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000302:isolation & purification*; D004912:Erythrocytes; D005796:Genes; D007668:Kidney; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal; D012343:RNA, Transfer; D012834:Silver / Q000494:pharmacology; D014316:Tritium; D014981:Xenopus
D012335:RNA, Ribosomal; D014316:Tritium; D012834:Silver; D004247:DNA; D012343:RNA, Transfer