A quantitative method for the application of compressive forces to bone in tissue culture.
A quantitative method for the application of compressive forces to long bones in culture is described. Using this method it was found that a physiological pressure of 80g/cm sq. applied to tibiae of 16-day-old chick embryos reduced glucose consumption to 50% of controls. Twenty four hours after the release of pressure glucose utilization again increased, approaching control levels. A pressure of 80 g/cm sq. also stimulated thymidine incorporation into DNA. The same pressure decreased the size of the extracellular fluid pool by 8%, but had no effect on the rate of equilibration of this pool with 22Na.
D000818:Animals; D001345:Autoradiography; D001692:Biological Transport; D001846:Bone Development; D001842:Bone and Bones / Q000378:metabolism*; D002642:Chick Embryo; D004247:DNA / Q000096:biosynthesis; D005110:Extracellular Space / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D008722:Methods; D009924:Organ Culture Techniques; D011312:Pressure; D013314:Stress, Mechanical; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D005947:Glucose; D013936:Thymidine