Radioimmunoassay of chlordiazepoxide in plasma.
A simple, rapid, and sensitive radioimmunoassay was developed for the determination of chlordiazepoxide (7-chloro-2-methylamino-5-phenyl-3H-1,4-benzodiazepine 4-oxide) in plasma. Antiserum capable of binding chlordiazepoxide-2-14C was obtained from rabbits following immunization with an antigen prepared by coupling the reactive acyl azide of 7-chloro-5-(4-hydrazinocarbonylmethoxyphenyl)-2-methylamino-3H-1,4-benzodiazepine 4-oxide to bovine serum albumin. The radioimmunoassay allows for the specific determination of chlordiazepoxide directly in plasma without extraction and was compared with a differential spectrofluorometric assay for chlordiazepoxide. Both methods gave satisfactory agreement for the plasma levels of chlordiazepoxide in human subjects resulting from single and chronic oral doses of chlordiazepoxide hydrochloride.
D000284:Administration, Oral; D000818:Animals; D000918:Antibody Specificity; D002250:Carbon Radioisotopes; D002707:Chlordiazepoxide / Q000008:administration & dosage / Q000097:blood* / Q000276:immunology; D005260:Female; D006801:Humans; D007106:Immune Sera; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D012710:Serum Albumin, Bovine; D013050:Spectrometry, Fluorescence
D002250:Carbon Radioisotopes; D007106:Immune Sera; D012710:Serum Albumin, Bovine; D002707:Chlordiazepoxide