Formation of internucleotide 3'-5' phosphoramidate links by direct coupling of phosphoryl and amino groups.
The stepwise synthesis of oligomers derived from 5'-amino-5'-deoxythymidine 3'-phosphate and 5'-amino-5'-deoxy-3'-O-mono-p-methoxytrityl-thymidine is described. The internucleotide phosphoramidate links were formed by condensation of nucleoside 3'-phosphates with aminonucleosides by means of triphenylphosphine and dipridyl disulfide.
D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D004220:Disulfides; D006868:Hydrolysis; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis*; D010720:Phosphines; D011725:Pyridines; D013696:Temperature; D013942:Thymine Nucleotides / Q000138:chemical synthesis*
D004220:Disulfides; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D010720:Phosphines; D011725:Pyridines; D013942:Thymine Nucleotides