Estimation of urinary diamines and polyamines by thin-layer chromatography.
The dansylated derivatives of ammonia, 1,3-diaminopropane, 1,4-diaminobutane, 1,5-diaminopentane, spermidine, histamine, and spermine were separated by one ascending development in chloroform-triethylamine (5:1) on a 250-mum silica gel 60 plate. Putrescine, cadaverine, spermidine, and spermine in human urine were quantitated by a direct scan of the fluorescent intensity of the spots corresponding to these compounds. Higher amounts of spermidine and spermine were found in the urines of cancer patients compared to the values of these substances in normal urine.
D001943:Breast Neoplasms / Q000652:urine; D002103:Cadaverine / Q000652:urine; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds; D003959:Diamines / Q000652:urine*; D006801:Humans; D008545:Melanoma / Q000652:urine; D009101:Multiple Myeloma / Q000652:urine; D011073:Polyamines / Q000652:urine*; D011700:Putrescine / Q000652:urine; D013095:Spermidine / Q000652:urine; D013096:Spermine / Q000652:urine
D003619:Dansyl Compounds; D003959:Diamines; D011073:Polyamines; D013096:Spermine; D002103:Cadaverine; D013095:Spermidine; D011700:Putrescine