DNA reassociation kinetics and chromosome structure in the crabs Cancer borealis and Libinia emarginata.
DNA reassociation kinetics have been partly elucidated for the higher crabs C. borealis and L. emarginata, using calf thymus DNA as a standard. These crabs contain no detectable repeated DNA in the approximate multiplicity frequency range 2-100 copies, which is unusual for invertebrate DNAs. Each species contains a component renaturing at an intermediate rate, and also a very rapidly renaturing fraction. The very rapidly renaturing fraction is considerably larger than the cesium chloride-resolvable satellites of each species. The fraction reassociating at an intermediate rate includes sequences with a reiteration frequency of up to 9.0 X 10(4) copies. This is unusually high for invertebrate DNAs. The nearly exact correlation between kinetic complexity and independently determined haploid genome size leads to the conclusion that the most slowly renaturing sequences of both crab species are present only once per haploid genome. Therefore the chromatids of these species are uninemic structures, and there has been no detectable occurrence of polyploid speciation in the recent evolutionary history of either species.
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003386:Brachyura / Q000166:cytology*; D002845:Chromatography; D002875:Chromosomes; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D006238:Haploidy; D006882:Hydroxyapatites; D007700:Kinetics; D008297:Male; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D009696:Nucleic Acids
D004277:DNA, Single-Stranded; D006882:Hydroxyapatites; D009696:Nucleic Acids; D004247:DNA