Penicillium stoloniferum virus: altered replication in ultraviolet-derived mutants.
Phenotypic mutants of the wild type of Penicillium stoloniferum NRRL5267 were obtained from conidia exposed to ultraviolet light for 60 min (10% survival). Virus content of the wild type and of nine phenotypic mutants was determined by polyacrylamide gel electrophoresis. Four mutants had no detectable Penicillium stoloniferum virus F (PsV-F), whereas the other five had levels of PsV-F in the mycelium similar to the wild-type strain. All nine mutants and the wild type had comparable levels of Penicillium stoloniferum virus S (PsV-S). Maximum virus levels occurred after 9 days of submerged culture in a 2% yeast extract-15% sucrose medium. Virus replication in the fungal host continued after protein, RNA and DNA synthesis levelled off. Virus levels ranged from 85 to 150 E-260 units (extinction units at 260 nm in I cm cell) per 4-7 to 5-3 g dry weight of mycelium for the mutant strains compared to 106 E-260 units per 4-2 g dry weight of the wild-type strain.
D003116:Color; D004247:DNA / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000096:biosynthesis; D009154:Mutation; D010407:Penicillium / Q000378:metabolism / Q000528:radiation effects; D010641:Phenotype; D010942:Plant Viruses / Q000254:growth & development* / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D012328:RNA Viruses; D012367:RNA, Viral / Q000096:biosynthesis; D011830:Radiation Effects; D013172:Spores, Fungal / Q000528:radiation effects; D014466:Ultraviolet Rays; D014779:Virus Replication
D005656:Fungal Proteins; D012367:RNA, Viral; D012313:RNA; D004247:DNA