Automated high-pressure liquid chromatographic analysis of aspirin, phenacetin, and caffeine.
An automated high-pressure liquid chromatographic (HPLC) method for the separation and determination of aspirin, phenacetin, and caffeine in pharmaceutical dosage forms is descreibed. Separation of these compounds for quantitation is achieved on a controlled pore glass support, utilizing a mixture of acetic acid and chloroform as the mobile phase. The method is specific, accurate, and simple and provides for the quantitation of each chromatogram in a continuous fashion every 7 min. HPLC separation of other analgesics was studied on a spherical siliceous support. The feasibility of determining free salicylic acid in analgesics also was established.
D001241:Aspirin / Q000032:analysis*; D001322:Autoanalysis / Q000295:instrumentation; D002110:Caffeine / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D010615:Phenacetin / Q000032:analysis*
D002110:Caffeine; D010615:Phenacetin; D001241:Aspirin