Improved methods for production, isolation, and assay of two new chlorisoxazoline amino acid antitumor antimetabolites: U-42, 126 and U43, 795.
Improved fermentation and isolation procedures for antitumor antimetabolites U-42,126 and U-43,795 increased drug yields 30-fold. The sensitivity limit of a newly developed assay is 0.03 mug of U-42,126 and 2.0 mug of U-43,795 per ml. The in vitro antimicrobial effect of both drugs was antagonized by histidine.
D000903:Antibiotics, Antineoplastic / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology; D000963:Antimetabolites / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology; D005998:Glycine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology; D007555:Isoxazoles / Q000138:chemical synthesis / Q000302:isolation & purification; D008722:Methods; D010080:Oxazoles
D000903:Antibiotics, Antineoplastic; D000963:Antimetabolites; D007555:Isoxazoles; D010080:Oxazoles; D005998:Glycine