The effects of protriptyline and clomipramine in vitro on the uptake of 5-hydroxytryptamine and dopamine in human platelet-rich plasma.
The effects of protriptyline and clomipramine, at concentrations of 10(-7)M to 10(-4)M, were studied in vitro on the uptake of 5-hydroxytryptamine and dopamine uptake in human platelet-rich plasma. It was found that the tertiary amine, clomipramine, was a more potent inhibitor of 5-hydroxytryptamine uptake than the secondary amine, protriptyline. The activity of both compounds was competitive but it was thought unlikely that they acted through tryptamine receptor sites as methysergide 2.5 X 10(-8)M had very little effect on 5-hydroxytryptamine uptake. Neither tricyclic antidepressant had any marked effect on dopamine uptake.
D001667:Binding, Competitive; D001692:Biological Transport / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002997:Clomipramine / Q000494:pharmacology*; D003864:Depression, Chemical; D003984:Dibenzazepines / Q000494:pharmacology*; D003986:Dibenzocycloheptenes / Q000494:pharmacology*; D004298:Dopamine / Q000097:blood*; D006801:Humans; D008784:Methysergide / Q000494:pharmacology; D011530:Protriptyline / Q000494:pharmacology*; D012701:Serotonin / Q000097:blood*
D003984:Dibenzazepines; D003986:Dibenzocycloheptenes; D012701:Serotonin; D011530:Protriptyline; D002997:Clomipramine; D004298:Dopamine; D008784:Methysergide