[Blast transformation of the lymphocytes of the blood of Macaca rhesus in culture by means of stimulation with phytohemagglutinin and allogenic leukocytes (methods of cultivation and quantitative evaluation of blast transformation)].
The authors consider a number of methodical questions of great significance for the brief-term cultures of both human and animal lymphocytes. A method of assessment of blast transformation of lymphocytes by radiometric measurement of H-3-thymidine incorporated into the cells was elaborated. A heterogenic system trichloracetic acid-insoluble cell fraction on a millipore filter--was used for measuring the tritium irradiation.
D000818:Animals; D001639:Bicarbonates; D002417:Cattle; D003470:Culture Media; D005994:Glycerophosphates; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D007962:Leukocytes / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008253:Macaca mulatta; D013936:Thymidine; D014316:Tritium; D014325:Tromethamine
D001639:Bicarbonates; D003470:Culture Media; D005994:Glycerophosphates; D037102:Lectins; D014325:Tromethamine; D014316:Tritium; D013936:Thymidine
Blastnaia transformatsiia limfotsitov krovi makak rezusov v kul'turakh, stimulirovannykh fitogemaggliutininom i allogennymi leĭkotsitami (metody kul'tivirovaniia i kolichestvennoĭ otsenki blastnoĭ transformatsii)