Mutations to azaguanine resistance induced in cultured diploid human fibroblasts by the carcinogen, N-acetoxy-2-acetylaminofluorene.
The ability of the carcinogen, N-acetoxy-2-acetylaminofluorene (N-AcO-AAF), to induce mutations to azaguanine resistance in diploid human cells was quantitatively investigated and shown to be dose-dependent. The 8-azaguanine (AG) resistance was shown to be heritable in the absence of mutagen or selective agent and the cells of the mutant clones were shown to retain normal sensitivity to N-AcO-AFF.
D000099:Acetoxyacetylaminofluorene / Q000494:pharmacology*; D001375:Azaguanine / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured / Q000187:drug effects*; D002999:Clone Cells / Q000187:drug effects; D003470:Culture Media; D004171:Diploidy; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D005347:Fibroblasts / Q000187:drug effects; D005449:Fluorenes / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D009154:Mutation
D003470:Culture Media; D005449:Fluorenes; D000099:Acetoxyacetylaminofluorene; D001375:Azaguanine