Production of 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin by Aspergillus caespitosus.
Aspergillus caepitosus Raper and Thom cultured on autoclaved cracked, yellow field corn produced 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin which was extracted and then purified by silica gel columns. The physical and spectral properties of the colorless crystals from methylene chloride:hexane were consistent with those published for 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin. Yield of high purity 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin was about 140 mg of corn per kg.
D001230:Aspergillus / Q000378:metabolism*; D002845:Chromatography; D003374:Coumarins / Q000096:biosynthesis*; D003470:Culture Media; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D009183:Mycotoxins / Q000096:biosynthesis*; D003313:Zea mays
D003374:Coumarins; D003470:Culture Media; D009183:Mycotoxins