Histochemical and biochemical evidence for the presence of microbodies in phytophthora palmivora.
Using the diaminobenzidine (DAB) reaction catalase activity could be demonstrated histochemically in cytoplasmic structures of Phytophthora palmivora bearing general ultrastructural features of microbodies. These socalled U-bodies sediment together with the catalase activity in Ficoll-Sorbitol-Sucrose gradients following prior purification by differential centrifugation.
D015100:3,3'-Diaminobenzidine; D002374:Catalase / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004796:Clinical Enzyme Tests; D005658:Fungi / Q000648:ultrastructure*; D006651:Histocytochemistry; D008830:Microbodies / Q000201:enzymology; D009940:Organoids; D010838:Phytophthora / Q000201:enzymology / Q000648:ultrastructure*; D013172:Spores, Fungal / Q000648:ultrastructure
D015100:3,3'-Diaminobenzidine; D002374:Catalase