Microbiological determination of drug partitioning. IV. Drug-protein interactions.
The protein binding characteristics of chloramphenicol, furazolium chloride, benzalkonium chloride, and phenylmercuric nitrate were described from their partitioning behavior in gelatin-acacia complex coacervate systems. Although the partitioning was determined by two different methods (microbiological and chemical), the microbiological method was more reliable for this type of investigation. Drug-protein parameters were calculated for the four antimicrobials. The advantages of the coacervate systems over other models for protein binding studies of drugs are discussed.
D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001548:Benzalkonium Compounds; D001681:Biological Assay; D002701:Chloramphenicol; D005664:Furazolidone; D008628:Mercury; D009581:Nitrofurans; D009942:Organometallic Compounds; D004364:Pharmaceutical Preparations; D011485:Protein Binding; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D012995:Solubility; D013844:Thiazoles
D001548:Benzalkonium Compounds; D009581:Nitrofurans; D009942:Organometallic Compounds; D004364:Pharmaceutical Preparations; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D013844:Thiazoles; D005664:Furazolidone; D002701:Chloramphenicol; D008628:Mercury