[Stimulation by insulin of DNA synthesis in cultured mouse fibroblasts].
With the aid of autoradiography, the effect of insulin on entering S- from G1-period of the mitotic cycle and on the rate of DNA synthesis of the mouse fibroblasts (L), was studied,--in the cells incubated for 24 hr in serum-free medium. In these conditions the cells were temporarily blocked in G1-period. Insulin (100 mcU/ml) increased by 1.5-fold the amount of cells in S-period as well as caused a marked stimulation of DNA synthesis.
D000818:Animals; D001345:Autoradiography; D004247:DNA / Q000096:biosynthesis*; D007328:Insulin / Q000494:pharmacology*; D007739:L Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D013268:Stimulation, Chemical; D013936:Thymidine; D014316:Tritium
D007328:Insulin; D014316:Tritium; D004247:DNA; D013936:Thymidine
Stimuliatsiia insulinom sinteza DNK v kul'tiviruemykh myshinykh fibroblastakh