Determination of 17-monochloroacetylajmaline and its metabolite in plasma by TLC fluorescence detection.
A sensitive and specific method is described for the determination of 17-monochloroacetylajmaline (I) and its metabolite, ajmaline (II), in plasma. Method specificity is accomplished by combining an ion-pair extraction with chromatography followed by development and utilization of reaction product fluorescence of the isolated species on silica gel. Recovery of I and II added to plasma or water averaged 70%. The major loss in the assay resulted during a solvent evaporation step and was reproducible over the concentration interval studied. The limit of detectability for I is 0.06 mu-g/2 ml of plasma. The method was used to determine plasma levels of I and II in the dog following a dose of 10 mg/kg iv of I.
D000085:Acetates; D000404:Ajmaline / Q000031:analogs & derivatives* / Q000097:blood; D000447:Aldehydes; D000470:Alkaloids; D000818:Animals; D002713:Chlorine / Q000097:blood; D002855:Chromatography, Thin Layer; D003720:Densitometry; D004285:Dogs; D005453:Fluorescence; D009566:Nitrates
D000085:Acetates; D000447:Aldehydes; D000470:Alkaloids; D009566:Nitrates; D000404:Ajmaline; C008541:17-monochloroacetylajmaline; D002713:Chlorine