[Direct determination of inorganic phosphor in serum and urine utilizing Rhodamin B (author's transl)].
A micromethod is described for the spectrophotometric determination of inorganic phosphate in serum and urine utilizing Rhodamin B as a dye (phosphomolybdat-Rhodamin B complex), Brij 35, and polyvinylpyrrolidon as catalyst. The procedure does not involve deproteinization and yields a stable complex in 20 min. The linearity is constant up to at least 9 mg Pi per 100 ml. It is both accurate (r = 0.972 in serum, r = 0.989 in urine, recovery in urine 98%) and precise (vk = 1.16% in the series). Bilirubin up to 10 mg/100 ml and serum protein do not interfere with the method.
D001663:Bilirubin; D005069:Evaluation Studies as Topic; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods; D008832:Microchemistry; D008982:Molybdenum; D010710:Phosphates / Q000032:analysis* / Q000097:blood / Q000652:urine; D011205:Povidone; D012235:Rhodamines; D013053:Spectrophotometry; D013501:Surface-Active Agents
D007202:Indicators and Reagents; D010710:Phosphates; D012235:Rhodamines; D013501:Surface-Active Agents; D008982:Molybdenum; D011205:Povidone; D001663:Bilirubin
Bestimmung von anorganischem Phosphor in biologischem Material mit einem einstufigen Reagens, enthaltend Rhodamin B