Separation of the methylated nucleoside consituents of ribonucleic acid by two-dimensional thin-layer chromatography.
In an attempt to separate a number of methylated nucleoside constituents reportedly present in various mammalian RNA preparations, a comparison of the migration of selected nucleoside standards was assessed via thin-layer chromatographic techniques with respect to three solvent systems and an adsorbent of varying proportions of silica gel and microcrystalline cellulose. The results of these studies revealed that a mixture of seventeen methylated constituents could be separated following two-dimensional development on an adsorbent containing 60% cellulose and 40% silica gel. Constituents separated included the 2'-O-methylated derivatives of adenosine, guanosine, cytidine and uridine, 1-, N6- and N6,N6-methyladenosines, 1-methylinosine, 1-, 7-, N2- and N2,N2-methylguanosines, 3-, 4- and 5-methylcytidines and 3- and 5-methyluridines. In addition, all of the above constituents were separated from their respective non-methylated nucleosides and bases.
D000241:Adenosine / Q000032:analysis; D002855:Chromatography, Thin Layer; D003562:Cytidine / Q000032:analysis; D006151:Guanosine / Q000032:analysis; D008745:Methylation; D012263:Ribonucleosides; D012997:Solvents; D014529:Uridine / Q000032:analysis
D012263:Ribonucleosides; D012997:Solvents; D006151:Guanosine; D003562:Cytidine; D000241:Adenosine; D014529:Uridine