The aerobic metabolism of cyclohexanecarboxylic acid by Acinetobacter anitratum.
1. The aerobic metabolism of cyclohexanecarboxylic acid by a bacterium isolated from garden soil (Acinetobacter anitratum) was investigated. 2. Evidence for the formation of cyclohex-1-ene-1-carboxylate, 2-hydroxycyclohexanecarboxylate and pimelate when either cell suspensions or cell-free extracts were incubated in the presence of cyclohexanecarboxylic acid is presented. 3. Crude cell-free extracts required ATP, CoA, FAD and Mg2+ as cofactors for the production of pimelate from cyclohexanecarboxylic acid, suggesting the existence of an activating reaction with formation of CoA esters, in this system.
D000150:Acinetobacter / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000421:Alcaligenes / Q000378:metabolism*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003065:Coenzyme A / Q000378:metabolism; D003509:Cyclohexanecarboxylic Acids / Q000378:metabolism*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D008274:Magnesium; D010101:Oxygen Consumption; D010867:Pimelic Acids / Q000096:biosynthesis
D003509:Cyclohexanecarboxylic Acids; D010867:Pimelic Acids; D005182:Flavin-Adenine Dinucleotide; D000255:Adenosine Triphosphate; D008274:Magnesium; D003065:Coenzyme A