Z protein in hepatic uptake and esterification of long-chain fatty acids.
Fatty acids radioactivity was bound to Z protein in liver after administration of['3H]oleate to rats or to a perfused rat liver preparation.  Pretreatment withflavaspidic acid (340 mumol/kg), a potent inhibitor of fatty acid binding to hepatic Zprotein in vitri, effectively reduced oleate radioactivity bound to Z by 90.2 plusor minus 4.3% and 85.0 plus or minus 6.2% in the intact rat and perfused liver, respectively.  In spite of this effect, pretreatment of rats with flavaspidic acid did notalter plasma clearance, hepatic uptake, and esterification of ['3H]oleate.  In contrast, in the perfused liver preparation, infusion of flavaspidic acid (340 mumol/kg)or bromosulphalein (360 mumol/kg) increased uptake of ['3H]oleate at least twofold,and oleate esterification was decreased by 15-30%.  These results suggest that the binding of long-chain fatty acids to Z protein is not an obligatory step in their uptakeby the liver and that Z protein may be involved in fatty acid esterification.
D000818:Animals; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000378:metabolism*; D004952:Esters; D005227:Fatty Acids / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D008297:Male; D009829:Oleic Acids / Q000378:metabolism; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000494:pharmacology; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000502:physiology*; D051381:Rats; D013448:Sulfobromophthalein / Q000494:pharmacology
D004952:Esters; D005227:Fatty Acids; D009829:Oleic Acids; D011506:Proteins; D013448:Sulfobromophthalein; D010696:Phloroglucinol