[Enzymatic detection of carbamate pesticides on impregnated cellulose plates].
The detection of carbamate pesticides by an enzyme-inhibition method on impregnated (ethylene glycol, triethylene glycol, beta,beta'-oxydipropionitrile) cellulose thin layer plates is described. The enzyme-substrate combination: pig liver esterase-indophenylacetate (chromogenic substrate) was the most sensitive. The following parameters concerning the sensitivity of the method were investigated: enzyme source, substrate, pH, inhibition time, inhibition temperature, thickness of the cellulose layer, stationary phase.
D002219:Carbamates; D002482:Cellulose; D002800:Cholinesterase Inhibitors / Q000032:analysis; D002855:Chromatography, Thin Layer; D004950:Esterases; D007306:Insecticides / Q000032:analysis*; D008722:Methods
D002219:Carbamates; D002800:Cholinesterase Inhibitors; D007306:Insecticides; D002482:Cellulose; D004950:Esterases
Enzymatischer Nachweis von Carbamat-Pesticiden auf imprägnierten Cellulose-Platten