Biochemical and morphological studies of the short and long term survival of isolated nerve-endings.
The respiratory rate, potassium, amino acid, ATP and phosphocreatine content of cortical synaptosomes were measured over periods from one hour to 5 days. Incubation medium or tissue culture medium at 37 degrees C was employed. In suspensions, synaptosomes show diminished levels of all these parameters after 3 or 4 h, but synaptosome beds showed no loss of potassium, ATP or phosphocreatine at this time. Morphologically the synaptosomes showed good preservation of structure during the first 24 h in tissue culture. Respiratory response to electrical pulses was detectable even after 24 h in culture when basal respiration was low.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D046508:Culture Techniques; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000378:metabolism* / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010101:Oxygen Consumption; D010725:Phosphocreatine / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012694:Serine / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D005971:Glutamates; D007773:Lactates; D009419:Nerve Tissue Proteins; D010725:Phosphocreatine; D005973:Glutamine; D001224:Aspartic Acid; D012694:Serine; D005680:gamma-Aminobutyric Acid; D000255:Adenosine Triphosphate; D005947:Glucose; D011188:Potassium