A micropuncture study of the effect of isoprenaline on renal tubular fluid and electrolyte transport in the rat.
To explain the mechanism of the isoprenaline induced antidiuresis the effect of this substance on fluid and electrolyte reabsorption in various nephron segments of the rat kidney has been studied using micropuncture techniques. Isoprenaline (1.5x10-9mol/kg-min i.v.) decreased GFR of superficial nephrons and increased fractional proximal fluid, sodium and potassium reabsorption. However, the fractions of filtered fluid and electrolytes, which had been reabsorbed up to early distal tubule were unchanged after isoprenaline. This indicated that the increased fractional proximal reabsorption has been completely compensated by decreased reabsorption from Henle's loops. In the distal convoluted tubules an increase in the fractional fluid and sodium reabsorption could be established using a recollection technique. Given directly into the distal tubular lumen isoprenaline stimulated the isotonic fluid reabsorption, which was measured by the Gertz split oil droplet method. From these results it is concluded that the decrease in superficial GFR and the increased distal tubular fluid and sodium reabsorption lead to the decrease in urine volume and in urinary electrolyte excretion after isoprenaline.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D004231:Diuresis / Q000187:drug effects; D004573:Electrolytes / Q000378:metabolism; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007444:Inulin / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D007684:Kidney Tubules / Q000187:drug effects* / Q000502:physiology; D007686:Kidney Tubules, Distal / Q000187:drug effects; D007687:Kidney Tubules, Proximal / Q000187:drug effects; D008138:Loop of Henle / Q000502:physiology; D008297:Male; D011188:Potassium / Q000378:metabolism; D011677:Punctures; D051381:Rats; D012964:Sodium / Q000378:metabolism
D004573:Electrolytes; D007444:Inulin; D012964:Sodium; D007545:Isoproterenol; D011188:Potassium