Lipophilic O-antigens containing D-glycero-D-mannoheptose as the sole neutral sugar in Rhodopseudomonas gelatinosa.
Lipopolysaccharides (LPS, O-antigens) of 12 strains of the photosynthetic bacterium Rhodopseudomonas gelatinosa were obtained by the phenol/chloroform/petroleum ether method, recommended for extracting lipophilic glycolipids of enterobacterial R-mutants. All R. gelatinosa LPS have essentially the same chemical composition. Similar to LPS of Salmonella R-mutants of chemotypes Rd1 and Rd2, the sole neutral sugar constituent is an aldoheptose. The heptose of R. gelatinosa LPS has the D-glycero-D-manno- configuration, in contrast to the L-glycero-D-mannoheptose of enterobacterial LPS. 2-Keto-3-deoxyoctonate forms the acid-labile linkage between the lipid moiety (lipid A) and the oligosaccharide moiety of R. gelatinosa LPS. Like enterobacterial lipid A, lipid A of this species contains phosphate and D-glucosamine as the sole amino sugar. The fatty acid spectrum conprises beta-hydroxycapric, lauric, and myristic acids. Beta-Hydroxymyristic acid, the typical fatty acid of enterobacterial LPS, is lacking. The R. gelatinosa LPS show O-antigenic acitivity; passive hemagglutinations with untreated or heat-treated (not well alkali-treated) LPS and antisera prepared against heat-killed cells yield high titers. According to the serological cross-reactions observed, the LPS of the 12 strains could be arranged into two different serotypes: serotype I comprising strains 29/1, 29/2, 25/2, and serotype II comprising strains 44/K/6, 3/1, IS/10, 39/2, Dr2, 2150, P8P9, K32, P18f3.1. No serological cross-reactions were observed between LPS of these two different serotypes in passive hemagglutinations.
D002210:Caprylates / Q000032:analysis; D002854:Chromatography, Paper; D003429:Cross Reactions; D003652:Decanoic Acids / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D005990:Glycerol; D006386:Hemagglutination Tests; D006539:Heptoses / Q000032:analysis*; D007536:Isomerism; D007850:Lauric Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology*; D008358:Mannose; D009227:Myristic Acids / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D012241:Rhodopseudomonas / Q000032:analysis / Q000276:immunology*; D012703:Serotyping
D002210:Caprylates; D003652:Decanoic Acids; D005227:Fatty Acids; D006539:Heptoses; D007850:Lauric Acids; D008055:Lipids; D008070:Lipopolysaccharides; D009227:Myristic Acids; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D005944:Glucosamine; D005990:Glycerol; D008358:Mannose