Energy yield of denitrification: an estimate from growth yield in continuous cultures of Pseudomonas denitrificans under nitrate-, nitrite- and oxide-limited conditions.
The molar growth yields of Pseudomonas denitrificans, for nitrate, nitrite and nitrous oxide, were determined in chemostat culture under electron acceptor-limited conditions. Glutamate was used as the source of energy, carbon and nitrogen. The catabolic pattern was identical, irrespective of the terminal electron acceptors. The molar growth yields, corrected for maintenance energy, were 28-6 g/mol nitrate, 16-9 g/mol nitrite and 8-8 g/mol nitrous oxide. The energy yield, expressed on an electron basis, was proportional to the oxidation number of the nitrogen: nitrate (plus 5), nitrite (plus 3) and nitrous oxide (plus 1). It was concluded that oxidative phosphorylation occurs to a similar extent in each of the electron transport chains associated with the reduction of nitrate to nitrite, nitrite to nitrous oxide and nitrous oxide to nitrogen.
D000085:Acetates / Q000096:biosynthesis; D000693:Anaerobiosis; D002245:Carbon Dioxide / Q000096:biosynthesis; D002849:Chromatography, Gas; D004579:Electron Transport; D004734:Energy Metabolism; D005971:Glutamates / Q000378:metabolism; D013058:Mass Spectrometry; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D009609:Nitrous Oxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation; D011549:Pseudomonas / Q000254:growth & development* / Q000378:metabolism; D012044:Regression Analysis; D012988:Soil Microbiology; D013386:Succinates / Q000096:biosynthesis; D014871:Water Microbiology
D000085:Acetates; D005971:Glutamates; D009566:Nitrates; D009573:Nitrites; D013386:Succinates; D002245:Carbon Dioxide; D009609:Nitrous Oxide