Otimum kinetic enzymatic procedures for glucose and triglycerides in plasma and serum.
I describe optimum kinetic procedures for measuring glucose and triglycerides in plasma and serum by enzymatic techniques. Glucose was measured by the hexokinase method at a 1000-fold sample dilution. Absorbance and concentration are linearly related for concentrations up to 4 g/liter. Results are reproducible to about 25 mg/liter (standard error). Comparisons between the kinetic glucose method described here and an automated o-toluidine and a manual end-point method showed no apparent bias between the methods; correlation coefficients were 0.998 and 0.991, respectively. Triglycerides (triacylglycerols) were measured in a fully enzymatic system by measuring free glycerol after hydrolysis with lipase. Absorbance and concentration are linearly related to greater than 2.5 g/liter at a 300-fold sample dilution. Repeatability was about 30 mg/liter (standard error). Compared with a manual method, the correlation coefficient was 0.978, with a slope of 0.93.
D001786:Blood Glucose / Q000032:analysis*; D005990:Glycerol / Q000097:blood; D006593:Hexokinase; D006801:Humans; D007700:Kinetics; D008049:Lipase; D008722:Methods; D013053:Spectrophotometry; D014280:Triglycerides / Q000097:blood*
D001786:Blood Glucose; D014280:Triglycerides; D006593:Hexokinase; D008049:Lipase; D005990:Glycerol