Ageing in vivo and neuraminidase treatment of rabbit erythrocytes: influence on half-life as assessed by 51Cr labelling.
Old and young rabbit erythrocytes, separated by centrifugation, contained different respective activities of glucose-6-phosphate dehydrogenase, pyruvate kinase and acetylcholinesterase, and different quantities of stromal sialic acid. A systematic study of the survival rate of young and old erythrocytes incubated with different amounts of Vibrio cholerae neuraminidase is described. The half-life of intact old erythrocytes is significantly shorter than that of young erythrocytes with a similar sialic acid content.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002802:Cholinesterases / Q000097:blood; D002860:Chromium Radioisotopes; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000032:analysis / Q000187:drug effects*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D006207:Half-Life; D008297:Male; D009439:Neuraminidase / Q000494:pharmacology*; D011770:Pyruvate Kinase / Q000097:blood; D011817:Rabbits; D012794:Sialic Acids / Q000097:blood; D014734:Vibrio cholerae / Q000201:enzymology
D002860:Chromium Radioisotopes; D012794:Sialic Acids; D005954:Glucosephosphate Dehydrogenase; D011770:Pyruvate Kinase; D002802:Cholinesterases; D009439:Neuraminidase