Microassay of an arylamidase in needle biopsies of human liver.
A method is described for determination of liver arylamidase activity in homogenates of the small amount of human as well as experimental animal tissues (approx. 5-15 mg wet wt.) obtained by needle biopsy. One milligram of human liver contains 90-130 mug protein. The arylamidase activity in normal liver was 0.2 to 0.6 nmoles benzylhydrazine formed/mug protein/hr. The microdetermination of an arylamidase described in this paper is worth in the diagnosis of hepatic injury because of the use of biopsy sample.
D000626:Aminopeptidases / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001547:Benzaldehydes / Q000378:metabolism; D001593:Benzyl Compounds / Q000378:metabolism; D001706:Biopsy; D006801:Humans; D006834:Hydrazines / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D051381:Rats
D001547:Benzaldehydes; D001593:Benzyl Compounds; D006834:Hydrazines; D000626:Aminopeptidases