I. A study of the stages in the quantitative isolation of aminoacyl-tRNA synthetase activities from mouse liver.
The elution profiles of 17 aminoacyl-tRNA synthetases from chromatography of 149 000 x g supernatant on Sephadex G-200 were determined as well as the influence of different methods of homogenization and of chromatography on DEAE-cellulose on the elution profiles. With gentle homogenization all synthetases were eluted in the void volume in four different peaks, containing (a) leucyl- and phenylalanyl-, (b) lysyl-, prolyl-, isoleucyl-, methionyl-, glycl-, and valyl-, (c) arginyl-, alanyl-, and asparaginyl- and (d) aspartyl-, histidyl-, seryl-, threonyl-, glutaminyl-, and tyrosyl- tRNA synthetases. With less gentle homogenization, peaks of lower molecular weight appeared. More than two peaks for each aminoacyl-tRNA synthetases were never found. Of the aminoacyl-tRNA synthetases examined, alanyl-,arginyl-, aspartyl-, leucyl- and lysyl-tRNA synthetases were not inactivated by chromatography on DEAE-cellulose, whereas phenylalanyl- and seryl-tRNA synthetases lost 60% of their activity.
D000596:Amino Acids; D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D000818:Animals; D002250:Carbon Radioisotopes; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D008099:Liver / Q000201:enzymology; D051379:Mice; D014176:Protein Biosynthesis; D012260:Ribonucleases / Q000378:metabolism
D000596:Amino Acids; D002250:Carbon Radioisotopes; D012260:Ribonucleases; D000604:Amino Acyl-tRNA Synthetases