Endotoxin from Fusobacterium necrophorum of bovine hepatic abscess origin.
The endotoxic activity of Fusobacterium necrophorum bov 5 was investigated. The supernatant (S) fluid and cell wall (CW) preparation, obtained after differential centrifugation of the ruptured cell mass, were lethal for mice. The toxicity of the S fluid was stable during prolonged storage, treatment with formalin, and heating for 15 minutes at 80, 100, and 121 C, but was destroyed by alkaline hydrolysis with 0.25 N NaOH. The toxic factor was found in a high molecular weight (MW) fraction after gel filtration. The properties exhibited by the toxic S fluid resembled those of endotoxic lipopolysaccharide (LPS). Extracted and partially purified LPS (endotoxin) from F necrophorum bov 5 demonstrated a mouse median lethal dose (mouse LD50) of 16.8 mg/kg of body weight. The toxic LPS material, a high molecular weight moiety as estimated by gel filtration, was resistant to ribonuclease (RNase), deoxyribonuclease (DNase), and pronase treatment. A positive Shwartzman reaction (median skin lesion dose (SLD50) equal to 3.32 mug/kg of body weight) and biphasic fever response (minimal dose required to produce a fever index of 40 sq cm which falls on the linear portion of dose-response curve (FL40) equal to 0.41 mug/kg of body weight) further indicated the toxin was endotoxin in nature. The LPS from F necrophorum bov 5 was less toxic than Salmonella typhimurium LPS; but had considerable toxicity for experimental animals. The toxic activity of the partially purified F necrophorum bov 5 endotoxin was separated into 2 fraction regions by diethylaminoethyl (DEAE)-cellulose chromatography. The data provide evidence for the production of a potent endotoxin, possibly composed of more than one toxic component, which may be released upon cell disruption.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002473:Cell Wall / Q000032:analysis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004731:Endotoxins / Q000302:isolation & purification* / Q000633:toxicity; D005334:Fever / Q000139:chemically induced; D005557:Formaldehyde / Q000494:pharmacology; D005673:Fusobacterium / Q000032:analysis / Q000254:growth & development / Q000302:isolation & purification*; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000633:toxicity; D008100:Liver Abscess / Q000382:microbiology / Q000662:veterinary*; D051379:Mice; D011402:Pronase / Q000494:pharmacology; D011817:Rabbits; D012772:Shock, Septic / Q000209:etiology; D012790:Shwartzman Phenomenon / Q000209:etiology; D013696:Temperature; D013997:Time Factors
D004731:Endotoxins; D008070:Lipopolysaccharides; D005557:Formaldehyde; D011402:Pronase