Characterization of dog small intestinal fucolipids with human blood group H activity.
Fucolipids with human blood group H activity were isolated from several dog small intestines. On the basis of mass spectrometry, periodate oxidation, enzyme degradation, methylation, and immunologic studies the following structure is proposed: Fucalpha(1 yields2)Galbeta(1 yields 4)Glc-NAcbeta(1 yields 3)Galbeta(1 yields 4)Glc-ceramide. The ceramide was shown by mass spectrometry to contain hydroxyhexadecanoic acid and phytosphingosine as major consitutuents.
D000017:ABO Blood-Group System; D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D002849:Chromatography, Gas; D002854:Chromatography, Paper; D004285:Dogs; D005643:Fucose / Q000032:analysis* / Q000276:immunology; D005690:Galactose / Q000032:analysis; D005696:Galactosidases; D005944:Glucosamine / Q000032:analysis; D006017:Glycolipids / Q000276:immunology / Q000302:isolation & purification; D006596:Hexosaminidases; D006801:Humans; D007421:Intestine, Small / Q000032:analysis* / Q000276:immunology; D013058:Mass Spectrometry; D008968:Molecular Conformation; D009928:Organ Specificity; D010944:Plants / Q000201:enzymology; D011817:Rabbits / Q000276:immunology
D000017:ABO Blood-Group System; D006017:Glycolipids; D005643:Fucose; D005696:Galactosidases; D006596:Hexosaminidases; D005944:Glucosamine; D000117:Acetylglucosamine; D005690:Galactose