Immunopharmacological studies using 5-hydroxytryptamine antibody.
An antibody to serotonin (5-HT) was tested on several preparations in which 5-HT is considered to be involved. The antibody was capable of effectively competing with 5-HT receptor sites for free molecules of 5-HT. It inhibited the uptake of 3H-5HT by blood platelets and may explain the reduced endogenous 5-HT concentration in blood platelets of immunized rabbits. The decreased platelet content of 5-HT was substantiated by electron microscopic studies which showed a reduction in the number of 5-HT-containing subcellular storage organelles in platelets from these animals. The 5-HT antibody inhibited the 5-HT-induced contraction of aortic strips in a tissue bath preparation. When given intraventricularly, it inhibited for a time the sedative actions of reserpine thus adding new evidence to the often postulated role of 5-HT in the actions of reserpine.
D000262:Adenylyl Cyclases / Q000276:immunology; D000818:Animals; D000906:Antibodies; D001667:Binding, Competitive; D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D008297:Male; D008854:Microscopy, Electron; D009200:Myocardial Contraction / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D012110:Reserpine / Q000037:antagonists & inhibitors; D012701:Serotonin / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D012701:Serotonin; D012110:Reserpine; D000262:Adenylyl Cyclases