Studies on the contact sensitization of man with simple chemicals. IV. Timing of skin reactivity, lymphokine production, and blastogenesis following rechallenge with dinitrochlorobenzene using an automated microassay.
An automated microassay was adapted to the sutdy of specific in vitro lymphocyte transformation to dinitrochlorobenzene (DNCB) erythrocyte complexes (DNCB-antigen). Studies of culture conditions indicated that in flat-bottomed microtiter culture plates, 0.2ml culturese containing 4x105 leukocytes and a total culture time of 4 or 5 days yeild satisfactory results for the relatively low responses seen with specific antigens such as DNCB-antigen. Culture were incubated for 3 hr with tritiated thymidine and harvested with the Multipe Automated  Sample Harvester(MASH II). The effect of DNCB rechallenge on in vitro lymphocte transformation was stuided using this automated microassay. DNCB rechallenge boosted  the in vitro response to DNON 25  TO 31 MM Hg; four patients (hypercapnic, group II) at a co2 from 54 to57 mm Hg. AH 8165 0.25 mg/kg (one quarter of the dose required for intubation) was given by rapid central venous injection. Haemodynamic responses were similar in the two groups; these were increase in heart rate ranging from 45 to 60 percent, increases in mean arterial pressure of 17-20 pre cent, and increases in cardiac output of 22-32 per cent. The intensity of neuromusclar blockade of the forearm muscles after AH 8165 was similar in the two groups, and there was no significant difference in recovery rates; roup I patients were 80 per cent recovered in 36.6 min, group II patinets in 47.3 min. It was concluded that the activity of AH 8165 was not influenced by moderate changes in respiratory acid-base status.
D000941:Antigens; D003877:Dermatitis, Contact / Q000276:immunology*; D004137:Dinitrochlorobenzene / Q000494:pharmacology*; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008222:Lymphokines / Q000096:biosynthesis*; D008832:Microchemistry; D009578:Nitrobenzenes / Q000494:pharmacology*; D012867:Skin / Q000276:immunology*; D012879:Skin Physiological Phenomena; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000941:Antigens; D004137:Dinitrochlorobenzene; D008222:Lymphokines; D009578:Nitrobenzenes; D013936:Thymidine