GLC determination of heroin and its metabolites in human urine.
Heroin and its metabolites, 6-monoacetylmorphine, morphine, and normorphine, were determined in human urine with a GLC procedure. Heroin was extracted with chloroform at pH 4.5 and chromatographed at a temperature programmed from 200-250 degrees by 8 degrees/min. 6-Monoacetylmorphine and morphine were extracted with ethylene dichloride containing 30% isopropanol at pH 8.5, and normorphine was extracted at pH 10.4 wtih the same solvent. The extract was derivatized with trimethylsilylimidazole and chromatographed at 230 degrees for the determination of 6-monoacetylmorphine and morphine and at 220 degrees for normorphine and morphine.
D002849:Chromatography, Gas; D003932:Heroin / Q000031:analogs & derivatives / Q000652:urine*; D006801:Humans; D008722:Methods; D009020:Morphine / Q000652:urine
D003932:Heroin; D009020:Morphine