Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.
The level of deoxyadenylate (da) regions in human DNA was estimated from formation of poly(U)-poly(da) triplexes on nitrocellulose filters that were RNAase resistant. The (dA) rich sequences were determined following mild ribonuclease treatment of the poly(U)-DNA hybrids (5 mug/ml at 25 degreesC for 30 min), where as exhaustive ribonuclease treatment (5 mug/ml at 25 degrees C for 6 hr) estimated the more (dA) pure sequences. The level of (dA) rich regions was 0.39% of the DNA and for the more (dA) pure regions it was 0.07%. The (dA) regions were widely distributed throughout human DNA regardless of base composition or sequence repetition. However, a concentration of (dA) regions into main band CsC1 gradient fractions of DNA and into repeated DNA was observed.
D000227:Adenine Nucleotides / Q000032:analysis; D001940:Breast / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D004247:DNA / Q000032:analysis / Q000097:blood / Q000302:isolation & purification; D005260:Female; D006801:Humans; D006882:Hydroxyapatites; D008214:Lymphocytes / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010179:Pancreas / Q000201:enzymology; D010920:Placenta / Q000032:analysis; D011072:Poly U; D011247:Pregnancy; D012260:Ribonucleases; D014462:Ultrafiltration
D000227:Adenine Nucleotides; D006882:Hydroxyapatites; D011072:Poly U; D004247:DNA; D012260:Ribonucleases