Dipeptide transport in isolated intestinal brush border membrane.
Transport of glycyl-L-leucine into isolated brush border membrane vesicles was studied. On the basis of the following observations it was postulated that glycyl-L-leucine was transported intact by a specific dipeptide mechanism. (1) The differing time course and Na-+ stimulation of glycine, L-leucine and glycyl-L-leucine. (2) The failure of glycine and L-leucine to inhibit glycyl-L-leucine transport. (3) Initial presence of dipeptide within the vesicle. (4) Inhibition of glycyl-L-leucine uptake by other dipeptides. (5) The occurrence of accelerated amino acid uptake in the presence of the dipeptide.
D000818:Animals; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004151:Dipeptides / Q000378:metabolism* / Q000494:pharmacology; D005998:Glycine / Q000378:metabolism / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism / Q000494:pharmacology; D008353:Mannitol / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D013997:Time Factors
D004151:Dipeptides; D008353:Mannitol; D012964:Sodium; D007930:Leucine; D005998:Glycine