Development and evaluation of method for simultaneous determination of phenobarbital and diphenylhydantoin in plasma by high-pressure liquid chromatography.
A procedure for the simultaneous analysis of diphenylhydantoin and phenobarbital in plasma by high-pressure liquid chromatography was developed and evaluated. Separation is effected on a porous particle silicic acid column with chloroform dioxane-isopropanol-acetic acid (310:9.7:1.0:0.1 by volume) and is monitored at 254 nm. Results of the procedure were compared with results of a GLC assay.
D002845:Chromatography; D002849:Chromatography, Gas; D006801:Humans; D008722:Methods; D010634:Phenobarbital / Q000097:blood*; D010672:Phenytoin / Q000097:blood*
D010672:Phenytoin; D010634:Phenobarbital