Biosynthesis and cytokinin activity of 8-hydroxy and 2,8-dihydroxy derivatives of zeatin and N-6(increment-2-isopentenyl)adenine.
8-Hydroxy and 2,8-dihydroxy derivatives of the cytokinins, 6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine and N-6-(increment -2-isopentenyl)adenine, have been biosynthesized by xanthine oxidase oxidation. 8-Hydroxy derivatives have been shown to be the major intermdeiates. These compounds were tested for cytokinin activity in the tobacco bioassay. The results suggest that substitution of the 8 position with a hydroxyl group causes less decrease of cytokinin activity than substitution of both the 2 and 8 positions with hydroxyl groups.
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001681:Biological Assay; D002374:Catalase; D002417:Cattle; D003583:Cytokinins; D005260:Female; D008099:Liver / Q000201:enzymology; D013058:Mass Spectrometry; D008892:Milk / Q000201:enzymology; D008968:Molecular Conformation; D010944:Plants / Q000187:drug effects; D010947:Plants, Toxic; D011687:Purines / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D014026:Nicotiana / Q000187:drug effects; D014969:Xanthine Oxidase; D015026:Zeatin / Q000378:metabolism* / Q000494:pharmacology
D003583:Cytokinins; D011687:Purines; D015026:Zeatin; D002374:Catalase; D014969:Xanthine Oxidase; D000225:Adenine