Comparison of the induction course, biophysical chemical interactions and photochemical action spectra of phenobarbital and 3-methylcholanthrene induce hepatic microsomal P-450.
A comparison has been made of the physical and chemical properties of hepatic microsomal P-450 and associated enzyme systems from rats treated with phenobarbital or with 3-methylcholanthrene and other polycyclic aryl hydrocarbons. The results of these studies, though preliminary in nature, indicate clearly that the aryl-induced mixed-function oxidase systems differ significantly from the PB-induced ones in time course of induction, spectral properties, hyroxylase and demethylase activities, CO-inhibition of these reactions and light-reversal of the inhibition. The results support and extend the findings of other investigators regarding the differential biophysical and biochemical properties of aryl-induced systems and provide an experimental design for studying these properties in greater depth at the maximum of aryl induction.
D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D001711:Biotransformation; D001835:Body Weight; D002248:Carbon Monoxide / Q000494:pharmacology; D003061:Codeine / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004790:Enzyme Induction; D006591:Hexobarbital / Q000494:pharmacology; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D008797:Metyrapone / Q000494:pharmacology; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000032:analysis; D010084:Oxidation-Reduction; D010634:Phenobarbital / Q000494:pharmacology*; D010777:Photochemistry; D011083:Polycyclic Compounds / Q000494:pharmacology; D011335:Proadifen / Q000494:pharmacology; D051381:Rats; D013057:Spectrum Analysis; D013997:Time Factors
D000814:Aniline Compounds; D011083:Polycyclic Compounds; D008748:Methylcholanthrene; D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System; D011335:Proadifen; D006591:Hexobarbital; D006899:Mixed Function Oxygenases; D003061:Codeine; D010634:Phenobarbital; D008797:Metyrapone