A radioimmunoassay for chemically modified grass pollen allergen.
Because of the physicochemical properties of tyrosine adsorbed chemically modified grass pollen allergens neither the human skin test in atopic volunteers nor the inhibition of the radioallergosorbent test are suitable assay procedures. Radioimmunoassays involving a labelled IgG fraction of rabbit antisera to the modified materials can be developed. The dilution inherent in the solubilisation procedure for the tyrosine does not, in most cases, take the antigen concentration below the level of sensitivity of the assay. Assay of different batches of Pollinex with an inhibition assay shows that discernment of different dose levels is possible. The results are compared with a method of assaying total protein.
D000485:Allergens / Q000032:analysis*; D000818:Animals; D000937:Antigen-Antibody Reactions; D003488:Cyanogen Bromide; D004305:Dose-Response Relationship, Drug; D007073:Immunoglobulin E; D008722:Methods; D006109:Poaceae / Q000276:immunology*; D011058:Pollen; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012882:Skin Tests; D013997:Time Factors
D000485:Allergens; D007073:Immunoglobulin E; D003488:Cyanogen Bromide