Application of densitometry to the qualitative and quantitative evaluation of pharmaceutical colourants.
Samples from all the British manufacturers of food grade Amaranth and Sunset Yellow FCF were examined by a densitometric thin-layer chromatographic procedure which provides a rapid and convenient method for producing a qualitative and quantitative statement on the impurity profiles of dye materials. Wide variations in the impurities were found which could account for variations in toxicological results. The method could be incorporated into official specifications to control more precisely the quality of food grade dyes.
D000548:Amaranth Dye / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002855:Chromatography, Thin Layer / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis*; D003720:Densitometry / Q000379:methods*; D005505:Food Coloring Agents / Q000032:analysis* / Q000592:standards
D004396:Coloring Agents; D005505:Food Coloring Agents; D000548:Amaranth Dye