Application of nuclear magnetic resonance spectroscopy to proteins.
The active sites of enzymes can be studied in great detail using nuclear magnetic resonance spectroscopy. The determination of pKa values of active site histidine residues in bovine pancreatic ribonuclease and the characterization of the binding of peptide hormones to carrier proteins are two such examples. The study of the active site of staphylococcal nuclease is another example and is presented in detail in this paper. The structure of 3'5'-thymidine diphosphate bound in the active site of staphylococcal nuclease has been studied by measuring the relaxation rate enhancement of substrate analog nuclei by a paramagnetic metal ion. The lanthanide ion, Gd(III), was substituted for Ca(II) in the formation of the ternary complex of nuclease: Gd(III) : 3'5'-thymidine diphosphate. Measurements were made of the transverse relaxation rates of protons and the longitudinal and transverse relaxation rates of the phosphorus nuclei of bound nucleotide. Internuclear distances between the metal ion and atoms of the 3'5'-thymidine diphosphate nucleotide were determined from these data by using the Solomon-Bloembergen equation. In general, these distances corresponded closely to those determined by previous X-ray crystallography of the thymidine diphosphate complex. These internuclear distances were also used with a computer program and graphics display to solve for metal : nucleotide geometries which were consistent with the experimental data. A geometry similar to the structure of the metal : nucleotide complex bound to nuclease determined by X-ray analysis was one of the solutions to this computer modeling process. For staphylococcal nuclease the NMR and X-ray methods yield compatible high resolution information about the structure of the active site.
D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D005682:Gadolinium / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008836:Micrococcal Nuclease / Q000378:metabolism*; D009481:Neurophysins / Q000378:metabolism; D010121:Oxytocin / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D010649:Phenylalanine / Q000378:metabolism; D011485:Protein Binding; D012260:Ribonucleases / Q000378:metabolism; D013329:Structure-Activity Relationship; D013696:Temperature; D013942:Thymine Nucleotides / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D014667:Vasopressins / Q000378:metabolism; D014961:X-Ray Diffraction
D009481:Neurophysins; D013942:Thymine Nucleotides; D014667:Vasopressins; D014443:Tyrosine; D010649:Phenylalanine; D006639:Histidine; D010121:Oxytocin; D005682:Gadolinium; D012260:Ribonucleases; D008836:Micrococcal Nuclease; D002118:Calcium