Immunochemistry of hepatitis B surface antigen (HBsAg): preparation and characterization of antibodies to the constituent polypeptides.
The structural polypeptides of HBsAg were shown to be immunogenic in guinea pigs. Purified 22-nm forms of the ad and any subtypes of HBsAg were solubilized under reducing conditions and electrophoresed in SDS-polyacrylamide gels. Individual polypeptides isolated from both HBsAg/ad and HBsAg/ay subtypes were used to hyperimmunize guinea pigs using Freund's complete adjuvant. All animals produced specific antibodies against native HBsAg as determined by complement fixation, passive hemagglutination, and double-antibody radioimmunoprecipitation assays. Each polypeptide contained within its structure the group-specific HBsAg determinant, a. Equilibrium competitive inhibition studies were conducted to determine the relative affinities of antisera produced against the major HBsAg polypeptides P-1, P-2, and P-6 (23,000, 29,000, and 72,000 daltons, respectively).
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001667:Binding, Competitive; D002353:Carrier State; D002462:Cell Membrane / Q000276:immunology; D003168:Complement Fixation Tests; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006509:Hepatitis B / Q000276:immunology*; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens / Q000032:analysis*; D006801:Humans; D007106:Immune Sera; D007120:Immunochemistry; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D010455:Peptides / Q000302:isolation & purification; D011863:Radioimmunoassay; D012967:Sodium Dodecyl Sulfate; D013634:Tannins
D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens; D007106:Immune Sera; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D010455:Peptides; D013634:Tannins; D012967:Sodium Dodecyl Sulfate