Distribution of covalently bound and non-covalently bound secretory component on sbuclasses of rabbit secretory IgA.
The distribution of non-covalently bound secretory component (SC) on the two subclasses, IgA-f and IgA-g of rabbit secretory IgA (sIgA) was determined; the two subclasses were separated from each other by the use of antibody-immunosorbent columns and were subjected to SDS polyacrylamide gel electrophoresis. No SC appeared to be dissociated from the IgA-f molecules from each of 11 different rabbits; the IgA-g molecules, however, did have SC which was dissociated by SDS. Thus, all of the noncovalently bound SC on rabbit sIgA resides on the IgA-g subclass molecules.
D000818:Animals; D003126:Colostrum / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006041:Goats / Q000276:immunology; D007106:Immune Sera; D007070:Immunoglobulin A / Q000032:analysis*; D007128:Immunoglobulin Fragments / Q000032:analysis*; D007457:Iodine Radioisotopes; D008892:Milk / Q000276:immunology*; D008970:Molecular Weight; D009438:Neuraminic Acids; D011233:Precipitin Tests; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate
D007106:Immune Sera; D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments; D007457:Iodine Radioisotopes; D009438:Neuraminic Acids; D012967:Sodium Dodecyl Sulfate