Alkaline phosphatase modulation by osmolality changes during the growth cycle of KB cell cultures.
Growing KB cells in hyperosmolar medium causes in reduction in total alkaline phosphatase activity associated with a decrease in the proportion of the heat-labile and an increase of the heat-stable enzyme components. In standard medium enzyme activity increases progressively during a 6-day growth cycle and the proportion of heat-stable activity remains constant. In hyperosmolar medium, activity increases only during the initial 24 hr after the change in osmolality and then levels off, but the heat-stable alkaline phosphatase activity increases 7-fold within 48 hr. The transmission in thermostability is discernible 24 hr after increasing the osmolality of the medium and toward the end of a growth cycle most of the activity is heat-stable.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D002277:Carcinoma; D002455:Cell Division; D002460:Cell Line / Q000201:enzymology*; D003470:Culture Media; D006358:Hot Temperature; D006801:Humans; D009303:Nasopharyngeal Neoplasms; D009994:Osmolar Concentration; D013997:Time Factors
D003470:Culture Media; D000469:Alkaline Phosphatase