Experimental paracetamol-induced hepatic necrosis: a light- and electron-microscope, and histochemical study.
Rats were killed at various time-intervals up to 48 hr after a single large dose of paracetamol (3 g per kg) and their livers examined by light and electron microscopy. In general, this revealed glycogen depletion, loss of ribosomes, and cytoplasmic matrix swelling commencing 3-6 hr after administration which in centrilobular hepatocytes progressed to frank coagulative necrosis at 12-24 hr. Midzonal cells showed more prominent aqueous swelling with besiculation of the endoplasmic reticulum, and in some cells, gross hydropic vacuolation.
D000082:Acetaminophen / Q000009:adverse effects*; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006651:Histocytochemistry; D008099:Liver / Q000648:ultrastructure; D008107:Liver Diseases / Q000201:enzymology / Q000473:pathology; D008247:Lysosomes / Q000648:ultrastructure; D008264:Macrophages / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains; D012270:Ribosomes / Q000648:ultrastructure; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013997:Time Factors; D014617:Vacuoles / Q000648:ultrastructure
D000082:Acetaminophen; D013385:Succinate Dehydrogenase; D000135:Acid Phosphatase