Messenger RNA-containing ribonucleoprotein from mitochondrial polyribosomes of rat liver.
A ribonucleoprotein was released from carefully purified rat liver mitochondrial polyribosomes after dissociation with 1 M potassium chloridepuromycin. This ribonucleoprotein was characterized by a sedimentation coefficient ranging from 10-14 S and buoyant density of 1.48 g cm(-3) in cesium chloride equilibrium centrifugation differing in these parameters from the subunits of mitochondrial ribosomes. Poly(A)-containing RNA constituted more than 30% of the total RNA content in this non-ribosomal ribonucleoprotein.
D000818:Animals; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D008930:Mitochondria, Liver / Q000032:analysis*; D008970:Molecular Weight; D009698:Nucleoproteins; D011061:Poly A / Q000032:analysis; D011132:Polyribosomes / Q000032:analysis* / Q000648:ultrastructure; D011485:Protein Binding; D011691:Puromycin; D012333:RNA, Messenger; D051381:Rats; D012261:Ribonucleoproteins
D009698:Nucleoproteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D011061:Poly A; D011691:Puromycin