Rapid citrate-agar electrophoresis in routine screening for hemoglobinopathies using a simple hemolysate.
A system for rapid hemoglobin electrophoresis on both cellulose acetate and citrate-agar combined with a simple solubility test for sickling variants is described. By use of a saponin hemolysate of once-washed erythrocytes, all three tests can be performed at one time so that an accurate result of the hemoglobin phenotype can be provided expeditiously. A technic for rapid hemoglobin electrophoresis on citrate-agar, using standard equipment is described in detail.
D001774:Blood Chemical Analysis; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate; D004912:Erythrocytes / Q000032:analysis*; D006453:Hemoglobinopathies / Q000175:diagnosis*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans
D006454:Hemoglobins