Hemocyanin - antibody labeling of phodopsin in mouse retina for a scanning electron microscope study.
To reveal the presence of rhodopsin on the surface of the mouse retina, a scanning electron microscope study of the immunolabeling of rhodopsin was attempted. The glutaraldehyde-fixed mouse retina was treated first with rabbit antibodies specific against bovine rhodopsin and then with hemocyanin-labeled goat antibodies specific against rabbit antibody. The distribution of hemocyanin label on mouse retina and the technique used for labeling are discussed.
D000818:Animals; D001666:Binding Sites, Antibody; D055598:Chemical Phenomena; D002621:Chemistry; D006041:Goats / Q000276:immunology; D006433:Hemocyanins; D006651:Histocytochemistry; D007074:Immunoglobulin G; D008722:Methods; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011817:Rabbits / Q000276:immunology; D012160:Retina / Q000032:analysis* / Q000648:ultrastructure; D012168:Retinal Pigments / Q000032:analysis*; D012243:Rhodopsin / Q000032:analysis*
D007074:Immunoglobulin G; D012168:Retinal Pigments; D012243:Rhodopsin; D006433:Hemocyanins