A biologically active hormonal fragment isolated from bovine parathyroid glands (BPTH 1-65).
Fresh frozen bovine parathyroid glands were defatted in acetone, when extracted with phenol. Following trichloroacetic acid precipitation, the resultant peptides were chromatographed on Sephadex G-100. Parathyroid hormone (BPTH) characteristically elutes in the fourth peak. However, we also observed significant hormonal activity, both biological and immunological, in the fifth elution peak. The peak V material had potent hypercalcemic activity in the rat and chick, and stimulated adenylate cyclase activity in the rat renal cortex bioassay. This material was further purified by ion exchange chromatography on carboxymethylcellulose in 8 M urea. The biological activity of the purified peptide (3700 MRC units/mg) was equivalent to that of the native hormone on a molar basis. Amino acid analysis, carboxypeptidase digestion, and partial Edman sequence analysis identified this material as BPTH 1-65, a hormonal fragment lacking the C-terminal 19 residues of the 84 residue hormone molecule. Several immunoassays using different anti-PTH antisera had variable reactivity toward the BPTH 1-65 fragment, showing that it may be useful for further characterizing antibody recognition sites. The presence of a lysine residue at position 65 suggests a tryptic-like cleavage may be responsible for the genesis of this hormonal fragment. Further investigation will be necessary to determine if this peptide has physiological significance.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D002417:Cattle; D002645:Chickens; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004589:Electrophoresis, Disc; D006934:Hypercalcemia / Q000139:chemically induced / Q000378:metabolism; D007672:Kidney Cortex / Q000187:drug effects; D010280:Parathyroid Glands / Q000032:analysis*; D010281:Parathyroid Hormone / Q000302:isolation & purification / Q000494:pharmacology; D010446:Peptide Fragments / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D014357:Trypsin
D000596:Amino Acids; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D014357:Trypsin