Rapid purification of tri-iodothyronine and thyroxine protein conjugates for antibody production.
Thyroxine (T-4) and tri-iodothyronine (T-3) were coupled to human serum albumin (HSA) with carbodi-imide. By adsorption chromatography on Sephadex G-25, fractions containing purified conjugate, but not reversibly-bound T-3 or T-4, were obtained, and this procedure took 5 h; considerably less than the conventional dialysis technique. Highly specific high-titre antisera were produced in rabbits and guinea-pigs by injection of these fractions in Freund's adjuvant.
D000818:Animals; D000918:Antibody Specificity; D002234:Carbodiimides; D002850:Chromatography, Gel / Q000379:methods*; D003429:Cross Reactions; D006168:Guinea Pigs / Q000276:immunology; D007106:Immune Sera; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D012709:Serum Albumin; D013974:Thyroxine / Q000276:immunology / Q000302:isolation & purification*; D014284:Triiodothyronine / Q000276:immunology / Q000302:isolation & purification*
D002234:Carbodiimides; D007106:Immune Sera; D012709:Serum Albumin; D014284:Triiodothyronine; D013974:Thyroxine