Chromatography on lipophilic dextran gels for fractionation of low molecular weight compounds I: steroid digitonides.
A method for the separation of 3beta-hydroxysterols from other sterols is presented. The method involves precipitating 3beta-hydroxysterols as the digitonides. The digitonide is then decomposed and separated into components using chromatography on highly cross-linked lipophilic polysaccharide gels. The digitonin in the mother liquor is separated from other sterols using the same chromatographic procedure.
D002845:Chromatography / Q000379:methods; D003911:Dextrans; D004071:Digitalis Glycosides / Q000302:isolation & purification*; D004072:Digitonin / Q000302:isolation & purification; D005782:Gels; D006914:Hydroxysteroids / Q000032:analysis; D008970:Molecular Weight; D012997:Solvents; D013261:Sterols / Q000302:isolation & purification*; D013265:Stigmasterol / Q000302:isolation & purification; D014316:Tritium
D003911:Dextrans; D004071:Digitalis Glycosides; D005782:Gels; D006914:Hydroxysteroids; D012997:Solvents; D013261:Sterols; D014316:Tritium; D013265:Stigmasterol; D004072:Digitonin