Physiological and biochemical effects of the mycotoxin patulin on Chang liver cell cultures.
Patulin exhibits both cytotoxic and cytopathic effects on cultured Chang liver cells. The LD50 found was 1.85 mug per ml of patulin. Effects on growth were observed with as little as 0.1 mug per ml of patulin; a 50% reduction in growth was observed at 0.38 mug per ml of patulin. Using a challenge dose of 2.5 mug per ml of patulin, the cytotoxic effect was reversible after an exposure of 10 min, but was not reversible after 20 min. Protein synthesis was depressed after 60 min and RNA synthesis after 20 min of contact with patulin. Neither protein nor RNA synthesis was completely inhibited after 260 min.
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007928:Lethal Dose 50; D008099:Liver; D009694:Nucleic Acid Precursors / Q000378:metabolism; D010365:Patulin / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011714:Pyrans / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D013997:Time Factors
D009694:Nucleic Acid Precursors; D011714:Pyrans; D012335:RNA, Ribosomal; D012313:RNA; D010365:Patulin