Colorimetric and gas-liquid chromatographic determination of atropine-hyoscyamine and hyoscine (scopolamine) in solanaceous plants.
Two colorimetric micromethods are described for the determination of atropine-hyoscyamine and hyoscine (scopolamine), using p-dimethylaminobenzaldehyde and citric acid-acetic anhydride as the color reagents. These methods are sensitive to 60-1200 and 10-360 mug alkaloid/10 ml. The colorimetric methods were also successfully applied after a preliminary thin layer chromatographic separation of the alkaloids. A gas-liquid chromatographic procedure was also developed, which yielded comparable results with the colorimetric methods.
D001285:Atropine / Q000032:analysis*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003124:Colorimetry / Q000379:methods*; D008832:Microchemistry; D010946:Plants, Medicinal / Q000032:analysis; D012601:Scopolamine / Q000032:analysis*
D001285:Atropine; D012601:Scopolamine