Thin layer chromatographic separation and spectrodensitometric determination of higher and lower sulfonated subsidiary dyes in FD&C Yellow No. 6.
A thin layer chromatographic method is presented by which higher and lower sulfonated subsidiary dyes are simultaneously separated from FD&C Yellow No. 6. After separation, the colors are quantitated directly, using a spectrodensitometer. Recoveries of 1, 2, and 5% of added 1-(4-sulfophenylazo)-2-naphthol-3,6-disulfonic acid ranged from 92 to 111%. Recoveries of 1, 2, and 5% of added 1-(4-sulfophenylazo)-2-naphthol ranged from 90 to 110%.
D001391:Azo Compounds / Q000032:analysis*; D001557:Benzenesulfonates / Q000032:analysis; D002855:Chromatography, Thin Layer; D004396:Coloring Agents / Q000032:analysis*; D003720:Densitometry; D005505:Food Coloring Agents / Q000032:analysis*; D009282:Naphthalenesulfonates / Q000032:analysis*; D013057:Spectrum Analysis
D001391:Azo Compounds; D001557:Benzenesulfonates; D004396:Coloring Agents; D005505:Food Coloring Agents; D009282:Naphthalenesulfonates