[Preparation and standarization of a Brucellosis allergen of sheep and goats].
The authors underline the consequences upon national economy and public health of sheep and goat brucellosis and expose the difficulties existing with the serological methods of diagnosis presently in use. In order to eliminate these disadvantages they propose the use of the intradermal allergic test reporting, at the same time, the reasons for which this test has not been until now universally adopted : lack of a standard allergen, always prepared by the same method and possessing excellent qualities. To avoid existing difficulties, they propose the use of a new allergen based on an original method of preparation, resulting from a careful study of the subject. They describe the method of preparation of the product and expose the different laboratory control tests on albinos guinea pigs. The excellent results obtained allow to conclude that the brucella melitensis strain used is suitable for the preparation of the allergen as well as for the sensitization of guinea pigs. The best sensitization method is the double subcutaneous injection of viable organisms in emulsion with Freund's adjuvant : one billion for the first and two billions for the second injection, at twenty days interval. Activity control of the allergen by intradermal inoculation of dilutions 1/10, 1/1001/1000, fifteen days after the last injection, gives the best results. Reading of the allergic reactions should take place twenty-four hours after the intradermal inoculation of the allergen because of rapid decrease in dimensions and intensity. The allergen demonstrates exceptional potency and specificity qualities; it may be considered as a "purified" product : all the tests on the control guinea pigs are negative, there is no formation of agglutinins nor sensitization of these laboratory animals towards the product itself.
D000485:Allergens / Q000302:isolation & purification* / Q000592:standards; D000818:Animals; D002002:Brucella / Q000032:analysis / Q000276:immunology; D002006:Brucellosis / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006041:Goats / Q000276:immunology; D006168:Guinea Pigs / Q000276:immunology; D008722:Methods; D008832:Microchemistry; D012756:Sheep / Q000276:immunology
D000485:Allergens
Préparation et standardisation d'un allergène brucellique pour les moutons et les chèvres