The separation of 9 S RNA from avian immature red blood cells into f2c-histone mRNA and globin mRNA.
9 S RNA from avian immature red blood cells was isolated from polysome-released ribonucleoprotein particles by sucrose-gradient techniques. Translation of this RNA in an Ehrlich ascites cell-free system and product analysis revealed that globin mRNA was contaminated by f2c-histone mRNA. When 9 S RNA was applied to oligo(dT)-cellulose columns a partial separation could be achieved. Poly (A)-containing globin mRNA did not contain f2c-histon mRNA, whereas the RNA which was not absorbed to oligo(dT)-cellulose contained all the f2c-histone mRNA besides substantial amounts of globin mRNA.
D000818:Animals; D001717:Birds; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002467:Cell Nucleus / Q000032:analysis; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000032:analysis* / Q000378:metabolism; D005914:Globins / Q000096:biosynthesis*; D006657:Histones / Q000096:biosynthesis*; D007930:Leucine / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D011132:Polyribosomes / Q000032:analysis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D012335:RNA, Ribosomal / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism
D006657:Histones; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D005914:Globins; D007930:Leucine; D008239:Lysine