The attacker: target-cell ratio and serum effects on in vitro cell-mediated immunity.
Sera collected from mice rejecting an allogeneic tumor and showing arming activity for normal splenocytes increased target-cell lysis by immune cells when low (less than or equal to 25:1) attacker:target-cell ratios (A:T) were used but inhibited cytotoxicity at higher (100:1) A:T. The importance of this methodologic variable in studies of serum activity on cellular cytotoxicity is emphasized and the possible mechanisms are discussed.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D005260:Female; D007111:Immunity, Cellular; D007939:Leukemia L1210 / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H