Characterization of monosomes produced by aflatoxin B1.
A single injection of 1.5 mg aflatoxin B1 per kg body weight produced approx. 70% disaggregation of rat liver polysomes into monosomes within 18 h. Isolated monosomes dissociated into 40 S subunits during centrifugation in linear sucrose gradients containing 0.3 M KCI. The 4 S to 5 S molar RNA ratio of the monosomes was calculated to be 0.6, indicating 0.6 tRNA and/or aminoacyl tRNA molecule per ribosome; no peptidyl tRNA was present. These results suggest that a single injection of affatoxin B1 produces monosomes which resemble runoff ribosomes.
D000348:Aflatoxins / Q000494:pharmacology*; D000818:Animals; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D008722:Methods; D011132:Polyribosomes / Q000187:drug effects*; D012343:RNA, Transfer / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D012270:Ribosomes / Q000032:analysis / Q000187:drug effects
D000348:Aflatoxins; D012343:RNA, Transfer