Histocompatibility testing in dogs. I. A semi-micro mixed lymphocyte culture (MLC) technique for histocompatibility matching in dogs.
A semi-micro method for the mixed lymphocyte culture (MLC) test in dogs is described. Optimization of various factors influencing the test were invistigated. Discrimination between allogeneic and isogeneic cell mixtures was possible after 90 h or culture but a culture period of 144 h was found to be optimal. The method measures MLC identity or non-identity and can be used as a tool for histocompatibility testing of lymphocyte defined (LD) Antigens.
D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D006650:Histocompatibility Testing / Q000379:methods*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D011830:Radiation Effects; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D013936:Thymidine