Quantitative assay of diclofenac in biological material by gas-liquid chromatography.
Diclofenac and an internal standard are isolated from biological samples by acid-specific extraction and converted into the corresponding indolones with 0.5% sulphuric acid in 2,2,2-trifluorethanol. The neutral derivatives are isolated by solvent extraction and subjected to gas-liquid chromatography. The use of an electron capture detector permits the assay of diclofenac at levels down to 2 ng per sample.
D000893:Anti-Inflammatory Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas; D010648:Phenylacetates / Q000032:analysis* / Q000097:blood / Q000652:urine; D012997:Solvents
D000893:Anti-Inflammatory Agents; D010648:Phenylacetates; D012997:Solvents