The incorporation of ammonia nitrogen into albumin in man: the effects of diet, uremia and growth hormone.
15 N-ammonium chloride was given orally to 17 individuals in 20 studies. Retention of 15N by those with normal renal function was inversely proportional to the protein intake; enrichment of albumin with 15N increased during protein restriction. Protein restriction appeared to be a more potent stimulus to the synthetic use of ammonia than a large pool of urea nitrogen. Both retention of isotope and enrichment of albumin decreased when a high protein diet was given. Enrichment of albumin with 15N in response to 10 or 20 mg of growth hormone daily was similar to that in healthy individuals on a 20 g protein diet for 3 weeks. The relevance of these findings to the nutritional potential of recycled urea nitrogen is discussed.
D000328:Adult; D000643:Ammonium Chloride / Q000378:metabolism*; D001834:Body Water; D005243:Feces / Q000032:analysis; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology; D006801:Humans; D007674:Kidney Diseases / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes; D011241:Prednisone / Q000494:pharmacology; D011488:Protein Deficiency / Q000378:metabolism; D012709:Serum Albumin / Q000096:biosynthesis*; D014508:Urea / Q000378:metabolism; D014511:Uremia / Q000378:metabolism
D009587:Nitrogen Isotopes; D012709:Serum Albumin; D000643:Ammonium Chloride; D014508:Urea; D013006:Growth Hormone; D009584:Nitrogen; D011241:Prednisone