The sugar part of kappa-caseins from cow milk and colostrum and its microheterogeneity.
Cow kappa-casein contains only three different sugars (Gal, GalNAc, NeuNAc). However detailed analyses achieved mainly by gas liquid chromatography suggested a microheterogeneity at the sugar level. After alkaline borohydride treatment, filtration on Bio-Gel P4 and paper chromatography, different carbohydrate parts were obtained. The two main compounds had the following molar compositions: GalNAc (1), Gal(1) and NeuNAc (1) and GalNAc (1), Gal(1) and NeuNAc (2). From these data and our previous sequence studies, some formulae of the polysaccharide part were proposed. One of them was closely related to the sugar sequence of a glycopeptide with MN activity which was in agreement with our observation concerning a cross antigenic reactivity between the N blood group substances and the caseinoglycopeptides. All the polysaccharide parts isolated from colostrum caseinoglycopeptide were much more complex than those obtained from the normal glycopeptide, confirming an evolution of the sugar part as a function of time after parturition.
D000116:Acetylgalactosamine / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002364:Caseins / Q000032:analysis*; D002417:Cattle; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D003124:Colorimetry; D003126:Colostrum / Q000032:analysis*; D005260:Female; D005690:Galactose / Q000032:analysis; D008892:Milk / Q000032:analysis*; D011247:Pregnancy; D012794:Sialic Acids / Q000032:analysis
D002241:Carbohydrates; D002364:Caseins; D012794:Sialic Acids; D000116:Acetylgalactosamine; D005690:Galactose