Biosynthesis of 17beta-oestradiol in human breast carcinoma tissue and a novel method for its characterization.
Conversion of 7alpha3H-testosterone to 17beta-oestradiol by human mammary carcinoma tissue in vitro has been demonstrated. It was characterized unequivocally by conversion to 17beta-oestradiol-3-sulphate upon incubation with adenosine-3'-phosphate-5'-phosphosulphate and the highly specific enzyme oestrogen sulphotransferase.
D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism*; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D010724:Phosphoadenosine Phosphosulfate; D013466:Sulfurtransferases; D013739:Testosterone / Q000378:metabolism
D013739:Testosterone; D010724:Phosphoadenosine Phosphosulfate; D004958:Estradiol; D013466:Sulfurtransferases