[Incorporation of a 131-iodine-deoxycytidine monophosphate protamine complex into the DNA of tumor-bearing rats following partial synchronization with hydroxyurea].
The incorporation of 131-Iodo-deoxyuridine in rat tumours can be increased by partial synchronisation with hydroxyurea. The reported investigation was carried out to determine whether the incorporation could be further increased by using a basic 131-Iodo-deoxycytidine-monophosphate-protamine complex as a depot of Iodo-deoxyuridine. It was shown that the incorporation observed after synchronisation could be increased by using this complex (non-synchronized tumours 1,46% retention, synchronized tumours 2,28% retention; measured 4 hour after the final injection of hydroxyurea) whereas in a control group injected with 131-Iodo-deoxycytidine-monophosphate alone, only a small increase was observed. The large increase in activity in the liver and in the spleen (liver: non-synchronized 2,76%, synchronized 2,79% retention; spleen: non-synchronized 3,00, synchronized 3,80% retention; measured 4 hours after final injection of hydroxyurea) which is observed after injection of the 131-Iodo-deoxycytidine-monophosphate-protamine complex renders it unsuitable for use in the scintigraphic detection of tumours. 131-Iodo-deoxyuridine is shown to be more suitable for this purpose.
D000818:Animals; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D003841:Deoxycytidine / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism; D006918:Hydroxyurea / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008099:Liver / Q000378:metabolism; D008297:Male; D008938:Mitosis / Q000187:drug effects; D009455:Neurofibroma / Q000378:metabolism / Q000473:pathology; D009943:Organophosphorus Compounds; D011479:Protamines / Q000378:metabolism*; D011877:Radionuclide Imaging; D051381:Rats; D012513:Sarcoma, Experimental / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism
D004273:DNA, Neoplasm; D007457:Iodine Radioisotopes; D009943:Organophosphorus Compounds; D011479:Protamines; D003841:Deoxycytidine; D006918:Hydroxyurea
Einbau eines 131-Jod-Desoxycytidinmonophosphat-Protamin-Komplexes in die DNS von tumortragenden Ratten nach partieller Synchronisation mit Hydroxyharnstoff