Photodynamic effects of protoporphyrin on the architecture of erythrocyte membranes in protoporphyria and in normal red blood cells.
Protoporphyrin causes a photodynamic damage of the red blood cell membrane. After illumination of red blood cells in the presence of protoporphyrin three effects can be observed: 1. Red blood cell membranes show particle aggregation on the outer and inner fracture face, as seen in freeze-etch electron microscopy. 2. Electrophorograms of membrane proteins show an increasing protein association, not disrupted by sodium dodecyl sulfate. 3. The immunological response of A+ red cells to anti A serum is progressively retarded. It seems likely that these effects are interrelated consquences of the protoporphyrin-induced photodynamic membrane damage.
D002449:Cell Aggregation; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D003624:Darkness; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D005613:Freeze Etching; D006801:Humans; D008027:Light; D008854:Microscopy, Electron; D011164:Porphyrias / Q000097:blood*; D011166:Porphyrins / Q000494:pharmacology*; D011524:Protoporphyrins / Q000494:pharmacology*; D013997:Time Factors
D011166:Porphyrins; D011524:Protoporphyrins