Reprogramming in the absence of DNA synthesis in Galleria larval epidermis.
Larval epidermal cells from a day-1 penultimate instar Galleria larva on implantation into day-5 last instar larva metamorphose and deposit a pupal cuticle at the same time as the host pupates. DNA synthesis in the implanted larval cell was monitored with 3-H-thymidine. Various regimens of 3-H-thymidine application were used and under no conditions did the larval cells incorporate label during the period from implantation to deposition of pupal cuticle. This suggests that a wax moth larval ectoderm cell can reprogram its genome to secrete a pupal cuticle without a precedent cell division.
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D004261:DNA Replication; D007814:Larva; D008675:Metamorphosis, Biological; D009036:Moths; D011679:Pupa; D012867:Skin / Q000166:cytology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D013936:Thymidine