Cultivation of mycoplasma from conjunctiva and production of corneal immune response in guinea pigs.
Mycoplasma canis was the most frequent mycoplasmal isolant obtained from the conjunctival surface of the eyes of dogs. Nine mycoplasmal isolates, 5 were M canis, were recovered from the lower cul-de-sac of 101 dogs. Experimentally induced immune keratitis was produced by sensitizing guinea pigs to 3 antigen preparations of M canis and then administering intracorneal challenge inoculation. The guinea pigs were clinically observed by leukocyte migration-inhibition (LMI) technique and by histopathologic study of their corneas. The corneal reaction of the guinea pigs was not identical to a clinical entity of the dog.
D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage; D002464:Cell Migration Inhibition; D003228:Conjunctiva / Q000382:microbiology*; D003315:Cornea / Q000276:immunology*; D003318:Corneal Opacity / Q000276:immunology / Q000662:veterinary; D004285:Dogs / Q000382:microbiology; D005260:Female; D006168:Guinea Pigs; D007267:Injections; D007271:Injections, Intradermal; D007273:Injections, Intramuscular; D007634:Keratitis / Q000276:immunology / Q000662:veterinary*; D007962:Leukocytes / Q000276:immunology; D008297:Male; D009174:Mycoplasma / Q000276:immunology* / Q000302:isolation & purification; D012376:Rodent Diseases / Q000276:immunology*
D000942:Antigens, Bacterial