On the localisation of d-tubocurarine in rat liver lysosomes in vivo by electron microscopy and subcellular fractionation.
After i.v. injection in the rat, d-tubocurarine is taken up and concentrated by the liver. A method is developed for the visualisation of d-tubocurarine inside the liver cell by electron microscopy. Glutaraldehyde fixed liver blocks were immersed in an ammonium molybdate solution; d-tubocurarine was precipitated at sites of high concentration by molybdate, to form an insoluble d-tubocurarine-molybdate complex. This precipitate was found predominantly at the surface of lysosome-like particles, but also inside these organelles. In subcellular fractionation experiments, d-tubocurarine was found with a high relative specific "activity" in the lysosomal fraction, lending support to a lysosomal localisation of d-tubocurarine.
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002458:Cell Fractionation; D008099:Liver / Q000648:ultrastructure; D008247:Lysosomes / Q000032:analysis* / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008982:Molybdenum; D000644:Quaternary Ammonium Compounds; D051381:Rats; D013347:Subcellular Fractions / Q000032:analysis; D014316:Tritium; D014403:Tubocurarine / Q000032:analysis*
D000644:Quaternary Ammonium Compounds; D014316:Tritium; D008982:Molybdenum; D000135:Acid Phosphatase; D014403:Tubocurarine