Reversed phase chromatography of isoaccepting tRNA's from healthy and crown gall tissues from Nicotiana tabacum.
RPC 5 (Reversed Phase Chromatography) of aminoacyl-tRNA's from healthy and crown gall (induced by Agrobacterium tume-faciens strain B6) tobacco tissues were compared for eleven amino acids. For ten amino acids: alanine, arginine, glutamic acid, glycine, isoleucine, leucine, lysine, methionine, tyrosine, and valine, no qualitative or quantitative differences could be detected between aminoacyl-tRNA's from both sources. Phenylalanyl-tRNA's from crown gall tissues gave two peaks on RPC 5; the minor early eluting species (peak 1) was always absent in elution profiles of phenylalanyl-tRNA's from healthy tissues or from tobacco leaves. After the "Y" base was removed by pH 2.9 treatment, peak 2 of phenylalanine tRNA was shifted to the position of peak 1.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D046508:Culture Techniques; D010649:Phenylalanine; D010941:Plant Tumors / Q000032:analysis*; D010944:Plants / Q000032:analysis* / Q000201:enzymology; D010947:Plants, Toxic; D012343:RNA, Transfer / Q000302:isolation & purification*; D014026:Nicotiana / Q000032:analysis / Q000201:enzymology
D010649:Phenylalanine; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases