Preliminary observations on the mitogenic effect of cyclopropenoid fatty acids on rat pancreas.
Male Sprague-Dawley rats were fed a diet containing 500 ppm of cyclopropenoid fatty acids (CPFA). After 2 weeks, mitotic activity and [3H]thymidine incorporation into DNA were significantly increased in pancreatic acinar cells. Continued feeding of the diet for 4 and 8 weeks led to decreasing mitotic activity which was still significantly increased over that of control animals. Focal necrosis of acinar cells was histologically apparent after 8 weeks of CPFA ingestion. Ultrastructural evidence of focal cytoplasmic injury was detected in acinar epithelial cells as early as 2 weeks after feeding of the CPFA diet was begun. A difference in dose response to CPFA appears to exist between Sprague-Dawley and Fischer F344 rats in that the latter, a dose of CPFA (5 mg/100 g body weight), did not evoke a mitogenic response.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003521:Cyclopropanes / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004721:Endoplasmic Reticulum / Q000473:pathology; D005227:Fatty Acids / Q000494:pharmacology*; D008297:Male; D008854:Microscopy, Electron; D008934:Mitogens; D008938:Mitosis / Q000187:drug effects; D009336:Necrosis; D010179:Pancreas / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D051381:Rats; D013045:Species Specificity; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D003521:Cyclopropanes; D005227:Fatty Acids; D008934:Mitogens; D004247:DNA; D013936:Thymidine