Perfusion of human ovaries in vitro with artificial medium: metabolism and steroidogenesis.
A study was made of ovaries perfused in vitro with a liquid fluorocarbon that was stabilized in emulsion by a nonionic detergent to which tissue culture medium was added. A pulsatile flow was used in an organ preservation system under aseptic conditions. Glucose was utilized and lactate produced at a fairly constant rate for as long as 20 hours of perfusion. 14-C-progesterone and 14-C-17alphaOH-progesterone were synthesized from 14-C-acetate and estradiol was secreted. The production of these steroids by the perfused ovary appears to be responsive to gonadotropins.
D000085:Acetates / Q000378:metabolism; D000328:Adult; D001639:Bicarbonates / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007773:Lactates / Q000096:biosynthesis; D007986:Luteinizing Hormone / Q000494:pharmacology; D008875:Middle Aged; D010053:Ovary / Q000378:metabolism* / Q000502:physiology; D010477:Perfusion; D011374:Progesterone / Q000096:biosynthesis*; D013256:Steroids / Q000096:biosynthesis / Q000378:metabolism; D013997:Time Factors
D000085:Acetates; D001639:Bicarbonates; D006063:Chorionic Gonadotropin; D007773:Lactates; D013256:Steroids; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D005947:Glucose