Automated red cell antibody detection. An improved method.
A single-channel AutoAnalyzer was used to screen potential blood-grouping sera to exclude the presence of unwanted antibodies. In order to detect the widest possible range of antibodies, a low-ionic strength Polybrene method was employed, but difficulty was encountered because the aggregated cells adhered to the glass surfaces of the reaction coils, resulting in instability of the system. Modifications were made to the apparatus, and the improvements in the demonstration of antibodies that resulted are described.
D000017:ABO Blood-Group System; D000906:Antibodies; D001322:Autoanalysis / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology*; D006583:Hexadimethrine Bromide / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera; D007518:Isoantibodies / Q000032:analysis*; D011092:Polyethylene Glycols
D000017:ABO Blood-Group System; D000906:Antibodies; D007106:Immune Sera; D007518:Isoantibodies; D011092:Polyethylene Glycols; D006583:Hexadimethrine Bromide