Purification and chemical characterization of the major neurotoxin from the venom of Pelamis plautrus.
A major toxin was isolated from the venom of the sea snake Pelamis platurus (yellow-bellied sea snake) by Sephadex G-50 and carboxymethylcellulose column chromatography. The LD50 of the pure toxin (Pelamis toxin a) was 0.044 mug/g in mice representing a tenfold increase in toxicity after purification. The toxin was homogeneous in acrylamide disc gel electrophoresis and eluted as a single peak after isoelectric focusing in a sucrose density gradient column. The isoelectric point was 9.69; thus it is a highly basic protein. The toxin contained 55 amino acid residues with four disulfide linkages. When all disulfide linkages were reduced and alkylated, the toxic action of the pure toxin disappeared leading to the conclusion that the disulfide bonds of the neurotoxin were essential for toxic action.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004355:Drug Stability; D004589:Electrophoresis, Disc; D007525:Isoelectric Focusing; D007928:Lethal Dose 50; D051379:Mice; D008970:Molecular Weight; D012623:Seawater; D012910:Snake Venoms / Q000032:analysis*; D014118:Toxins, Biological / Q000302:isolation & purification / Q000494:pharmacology; D014688:Venoms / Q000032:analysis*
D000596:Amino Acids; D002241:Carbohydrates; D012910:Snake Venoms; D014118:Toxins, Biological; D014688:Venoms