Comparison of various cryoprotective agents on washed chicken spermatozoa. 5. Effect of glucose, sucrose and polyvinylpyrrolidone.
A study was conducted to determine the effect of glucose, sucrose and polyvinylpyrrolidone (PVP) on the viability of chicken spermatozoa. Levels of 4, 8 and 12% glucose, sucrose or 12% PVP significantly reduced the motility and fertilizing capacity of spermatozoa. Adding the compounds in bulk or fractions eliminated the drastic effect of 4% sucrose on motility and fertility but proved ineffective with 4% glucose or 12% PVP. A complete loss of motility with no effect on fertility was observed with 6% PVP was combined with either ethylene glycol or DMSO.
D000818:Animals; D002645:Chickens / Q000502:physiology*; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005298:Fertility / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D008297:Male; D011205:Povidone / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D013395:Sucrose / Q000494:pharmacology*
D003451:Cryoprotective Agents; D013395:Sucrose; D011205:Povidone; D005947:Glucose; D004121:Dimethyl Sulfoxide