Use of trimethylsilyl and other homologous trialkylsilyl derivatives for the separation and characterization of mono- and dihydroxycannabinoids by combined gas chromatography and mass spectrometry.
A gas chromatographic separation of dihydroxy- from monohydroxycannabinoids by the use of homologous trialkylsilyl derivatives is discussed. Trimethylsilyl derivatives produced a group of peaks containing both sets of compounds, sometimes poorly resolved, whereas by increasing the alkyl chain length to n-butyl complete fractionation into two groups, was achieved. The mass spectra of these derivatives resembled those of the trimethylsilyl derivatives with the addition of a set of ions resulting from estimation of the Si-alkyl chains as olefins.
D000081:Acetamides / Q000378:metabolism; D000097:Acetonitriles / Q000378:metabolism; D002188:Cannabis / Q000302:isolation & purification* / Q000378:metabolism; D002849:Chromatography, Gas; D004048:Diethylamines / Q000378:metabolism; D013058:Mass Spectrometry; D011725:Pyridines / Q000378:metabolism; D012825:Silicon; D014297:Trimethylsilyl Compounds / Q000378:metabolism
D000081:Acetamides; D000097:Acetonitriles; D004048:Diethylamines; D011725:Pyridines; D014297:Trimethylsilyl Compounds; D012825:Silicon