On the origin of desmethyl imipramine in rat brain.
The formation of DMI after incubation of IP-C-14 with supernatant fractions prepared from brain and liver of male Wistar rats were studied to determine the sources of DMI in brain tissue. IP and its metabolites were isolated by thin layer chromatography, eluted and counted in a Geiger-Muller counter. A substantial amount of DMI was formed when the liver supernatant fraction was used, whereas the brain fraction did not form DMI even at low level. Under the incubation condition used, about 2.7% of IP was converted non-enzymatically to a DMI-like substance. Based on the results presented it appears that at least most of the DMI reported by other authors to be formed in rat brain after IP administration, reaches the brain via the blood stream.
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D003891:Desipramine / Q000378:metabolism*; D007099:Imipramine / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051381:Rats
D007099:Imipramine; D003891:Desipramine