Stimulation of granulopoiesis by liver macrophages.
Isolated murine Kupffer cells or liver macrophages release high levels of colony-stimulating activity (CSA) necessary for the proliferation of granulocytic and mononuclear cell colonies in vitro.  CSA was detected in media conditioned by Kupffer cells ata concemtration of 0.25 x 10'6 cells per millilter.  Conditioned media from suspensions of unseparated liver did not stimulate colony formation and, in high concentrations,were inhibitory.  CSA obtained from Kupffer cells was more active on a cell percell basis than CSA OBTAINED FROM LUNG OR PERITONEAL MACROPHAGES.  These studies indicate that liver macrophages can also release CSA and further support the concept that the monocyte-macrophage system may play a role in the regulation of granulopoiesis.
D000818:Animals; D001853:Bone Marrow; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D006098:Granulocytes; D006410:Hematopoiesis; D006801:Humans; D007728:Kupffer Cells / Q000502:physiology; D007962:Leukocytes; D008099:Liver / Q000166:cytology*; D008168:Lung / Q000166:cytology; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D010537:Peritoneum / Q000166:cytology
D003470:Culture Media