Excretion of carbon-14-labeled aflatoxin B1 via bile, urine, and intestinal contents of the chicken.
Carbon-14-labeled aflatoxin B1 was prepared by growing Aspergillus parasiticus in medium containing sucrose-14-C as the sole carbon source. The 14-C-labeled aflatoxin B1 was extracted with chloroform and purified by thin-layer chromatography (TLC) followed by precipitation from solution and washing. The 14-C-labeled aflatoxin B1 was intravenously given to anesthetized dwarf White Leghorn or Rhode Island Red hens with cannulated ureters and bile ducts. The 14-C had a calculated plasma half-life of 1.5 minutes and rapidly appeared in the bile. The 14-C concentration in the bile reached values approximately 7 times maximum for plasma, and most could no longer be extracted with chloroform. This finding indicated that metabolites of the 14-C-labeled aflatoxin B1 had been excreted against a concentration gradient into the bile. The quantities of 14-C excreted via bile, urine, and intestinal contents remained at a fairly constant ratio of 70:15:15, respectively, over the 315-minute experimental period. Six major chloroform-soluble compounds containing 14-C were isolated. Three of these were tentatively identified as aflatoxin B1, B2, and M1. A 4th was believed to be aflatoxin B2a. None of the early chloroform-soluble metabolites were as cytotoxic as aflatoxin B1.
D000348:Aflatoxins / Q000097:blood / Q000378:metabolism* / Q000652:urine; D000818:Animals; D001646:Bile / Q000378:metabolism*; D001652:Bile Ducts; D001853:Bone Marrow / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D002404:Catheterization / Q000662:veterinary; D002645:Chickens / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D007413:Intestinal Mucosa / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D014546:Urinary Catheterization / Q000662:veterinary
D000348:Aflatoxins; D002250:Carbon Radioisotopes