Simultaneous application of pulsed high frequency currents and gamma-rays to cultured melanoma cells.
Cells differentiated from a human metastasizing melanoma have been irradiated simultaneously with pulsed high frequency currents (27.12 MHz, 400--600 pulses/s, pulse duration 65mus, average power 25--38 Watt) (Diapulse), and gamma radiation from a Co-60 source.  Simultaneous pulsed high frequency field and gamma radiation acted in a way resembling a cell-protecting mechanism, enhancing the number of surviving cells to the extent of about 15 percent as compared with the cell number scored after pure Co-60 gamma-radiation treatment.  Irradiation with pure high frequency currents resulted in increasing cell death for a period of up 4 minutes; subsequently, this seemed to be almost independent of time.  The number of cells which survived, as compared with the controls, was found to be approximately 80 percent, near a distance of 6.5 cm from the symmetry axis, perpendicular to the plane of the radiating element.
D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured / Q000528:radiation effects; D003037:Cobalt Radioisotopes / Q000627:therapeutic use; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D008545:Melanoma / Q000532:radiotherapy*; D011830:Radiation Effects; D011838:Radiation-Sensitizing Agents; D011867:Radioisotope Teletherapy; D012878:Skin Neoplasms / Q000532:radiotherapy; D013997:Time Factors; D014465:Ultrasonics
D003037:Cobalt Radioisotopes; D011838:Radiation-Sensitizing Agents