Kinetic determination of glucose with the GEMSAEC (ENI) centrifugal analyzer by the glucose dehydrogenase reaction, and comparison with two commonly used procedures.
A new glucose dehydrogenase preparation has been used to determine the glucose concentration in serum or plasma with the GEMSAEC(ENI) analyzer. The reaction is sufficiently linear to be suitable for a kinetic determination. A sample volume of 15 mul or less is needed, and 14 determinations can be done simultaneously within 160 s (the time needed for loading the samples and reagents into the distribution disc plus a reaction time of 70 s). The reaction is linear up to 300 mg of glucose per 100 ml, but with special computer software linearity could be extended to 1 g of glucose per 100 ml. Day-to-day and within-day precision have been tested with a number of different sera and standards. Accuracy has been checked by comparing the results with those obtained by the hexokinase and the glucose oxidase/peroxidase methods. A better agreement was found with the hexokinase method. The proposed procedure has the advantage of involving only one enzymatic step and of directly measuring reduced coenzyme formation at 340 nm.
D000429:Alcohol Oxidoreductases; D001322:Autoanalysis; D002498:Centrifugation; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006593:Hexokinase; D008722:Methods; D010544:Peroxidases
D000429:Alcohol Oxidoreductases; D005949:Glucose Oxidase; D010544:Peroxidases; D006593:Hexokinase; D005947:Glucose