[Collection of hematopoietic stem cells from canine peripheral blood and their ability to regenerate hematopoiesis].
A procedure is presented for the collection of a large number of hemopoietic stem cells from the peripheral blood of dogs by means of a single leukapheresis using the NCI-IBM Blood Cell Separator. In the course of a leukapheresis of about 285 min duration a mean of 23 x 10-9 leukocytes is collected from the blood. The hemopoietic stem cells among such separated leukocytes initiate repopulation of bone marrow within 10 days after whole body X-irradiation with 1200 R. The cell numbers in a defined histological section of femoral bone marrow are evaluated 9 to 10 days after irradiation and subsequent autologous transfusion of 6.72 x 10-9 separated mononuclear leukocytes. The results indicate that the bone marrow cell numbers of transfused dogs are significantly greater than in dogs given only 1200 R and reach a level of approximately 49% of the normal value. Possible ways of increasing the yield of hemopoietic stem cells from the peripheral blood will be considered.
D000818:Animals; D001804:Blood Transfusion, Autologous; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D004285:Dogs; D005269:Femur; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells; D007962:Leukocytes; D008297:Male; D011827:Radiation; D013997:Time Factors
Untersuchungen über die Gewinnung hämopoetischer Stammzellen aus dem peripheren Blut von Hunden und über ihre Fähigkeit, die Blutzellbildung zu regenerieren