[Hydrogenase activity in Thiocapsa roseopersicina according to the D2--H20 metabolic reaction].
Extracts of Thiocapsa roseopersicina cells show hydrogenase activity, measured by evolution of H2 from reduced methylviologene (MV) and by D2-H2O exchange reaction. According to these reactions the most part of hydrogenases is found to be in the soluble fraction. Hydrogenase activity measured in the exchange reaction is completely inhibited by p-chloromercurybenzoate (5-10- minus 3 M), iodacetate (1-10- minus 2 M) and 26% inhibited by KCN and o-phenanthroline (5-10- minus 3 M). Evolution of H2 from reduced MV was not inhibited by o-phenanthroline, KCN and iodacetate and was inhibited by 66% only with p-chloromercurybenzoate. Light and ATP stimulated hydrogenase activity of chromatophores did not affect on its activity in the soluble fraction. The results obtained show that there are certain differences in hydrogenase systems responsible for the exchange reaction and evolution of H2.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001419:Bacteria / Q000201:enzymology*; D002729:Chloromercuribenzoates / Q000494:pharmacology; D002849:Chromatography, Gas; D003470:Culture Media; D003486:Cyanides / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004794:Enzyme Repression / Q000187:drug effects; D006859:Hydrogen / Q000378:metabolism; D006865:Hydrogenation; D007461:Iodoacetates / Q000494:pharmacology; D008027:Light; D008365:Manometry; D010269:Paraquat / Q000378:metabolism; D010618:Phenanthrolines / Q000494:pharmacology; D011188:Potassium; D014867:Water / Q000378:metabolism*
D002729:Chloromercuribenzoates; D003470:Culture Media; D003486:Cyanides; D007461:Iodoacetates; D010618:Phenanthrolines; D014867:Water; D006859:Hydrogen; D000255:Adenosine Triphosphate; D010269:Paraquat; D011188:Potassium
Gidrogenaznaia aktivnost' Thiocapsa roseopersicina po reaktsii obemna D2-H20