A proton magnetic resonance investigation of the glycosyl torsion angle of uracil nucleosides and nucleotides.
The use of line-shape decomposition techniques permitted the small 5-bond (5-J51') and 4-bond (4-J61') proton-proton coupling constants of a series of uracil nucleosides and nucleotides to be determined accurately. From an analysis of these coupling constants we have determined that the uracil base is in a predominantly anti conformation in aqueous solution and the mean position is not substantially altered by phosphate substitution at the 2', 3', or 5' positions, by changing the furanose stereochemistry from a ribose to a deoxyribose or an arabinose, or by an increase in temperature of 43 degree C.
D001089:Arabinose / Q000031:analogs & derivatives; D003201:Computers; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D014500:Uracil Nucleotides; D014529:Uridine / Q000031:analogs & derivatives
D014500:Uracil Nucleotides; D001089:Arabinose; D014529:Uridine