Modification of polysaccharides containing uronic acid residues.
Klebsiella Type 47 capsular polysaccharide has side chains attached to the main chain via D-glucuronic acid residues. The side chains have been removed to yield an essentially linear polysaccharide by the following sequence of reactions: (1) substitution of hydroxyl and carboxyl groups with methyl vinyl ether; (2) beta-elimination by treatment with base; (3) removal of modified uronic acid residues and protecting groups by mild acid hydrolysis. The possibility of modifying other uronic acid-containing polysaccharides by this method is discussed.
D002849:Chromatography, Gas; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D005965:Glucuronates / Q000032:analysis*; D007709:Klebsiella / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008722:Methods; D008745:Methylation; D009904:Optical Rotation; D011135:Polysaccharides, Bacterial; D012210:Rhamnose / Q000032:analysis
D005965:Glucuronates; D011135:Polysaccharides, Bacterial; D005947:Glucose; D012210:Rhamnose; D005690:Galactose