Association of leucogenenol with specific proteins in serum.
Determination of the concentration of leucogenenol in the IgM, IgG, albumin, and low molecular weight protein fractions of serum demonstrated that leucogenenol is found only in protein fractions of molecular weights in the range of IgM and IgG. Determination of the distribution of leucogenenol in chromatographically separated serum proteins showed that leucogenenol has a maximum concentration in the proteins intermediate between those with the molecular weights of IgM and IgG. Comparison of the ratio of effluent to void volumes with the ratios of known proteins suggest that the associated or carrier protein of leucogenenol has a molecular weight of approximately 3 x 10-5. When additional leucogenenol is added to serum it is found in the same protein fractions as the leucogenenol normally present, indicating that there is excess carrier protein for leucogenenol in the serum.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis; D002850:Chromatography, Gel; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007932:Leucogenenol / Q000097:blood* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D012709:Serum Albumin / Q000032:analysis; D013141:Spiro Compounds / Q000097:blood*
D001798:Blood Proteins; D002352:Carrier Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012709:Serum Albumin; D013141:Spiro Compounds; D007932:Leucogenenol