Simplified photometric copper-soap method for rapid assay of serum lipase activity.
We modified the method of Yang and Biggs [Clin. Chem. 17, 512 (1971)] for greater speed and simplicity. Serum is incubated with an emulsified olive oil substrate, the fatty acids extracted, the copper soap formed, and phases separated, all in a single tube. An aliquot of the organic phase is removed for color development. One analysis can be completed in 35 min, 20 in less than 2 h. The method is accurate and precise. Results correlate well with the unmodified colorimetric method, and with the classical titrimetric lipase procedure. The reference interval (normal range) is 20 to 180 U/liter.
D000328:Adult; D003300:Copper; D005260:Female; D006801:Humans; D008049:Lipase / Q000097:blood*; D008297:Male; D009821:Oils; D010783:Photometry / Q000379:methods
D009821:Oils; D003300:Copper; D008049:Lipase