Distribution of troponin components in the thin filament studied by immunoelectron microscopy.
1. The localization of specific antiboidies against troponin components, i.e., troponin T(TN-T), troponin I(TN-I, and troponin C(TN-C), was studied by the use of an electrom microscope. 2. Every antibody was distributed along the thin filament with a period of 38 nm. 3. Staining with anti-TN-I or anti-TN-C formed narrow striations. The location of the first striation was 26 nm from the free end of the thin filament. 4. The width of individual striations formed by anti-TN-T was 14--20nm The H-band-side end of each striation coincided with the location of anti-TN-I or anti-TN-C.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera / Q000032:analysis; D008854:Microscopy, Electron; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000032:analysis* / Q000276:immunology; D013045:Species Specificity; D014336:Troponin / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D007106:Immune Sera; D009124:Muscle Proteins; D014336:Troponin