Identification of N-acylglycines by gas-liquid chromatography.
Glycine conjugates of aliphatic carboxylic acids of clinical interest, and a series of structurally related compounds, were synthesized. The gas chromatographic elution behavior of trimethylsilyl derivatives of these N-acylglycines was examined on columns of 5% OV-1. A single peak for each compound was observed on the chromatograms after derivatization with a reagent containing N,9-bis-(trimethylsilyl)-acetamide in pyridine (1 : 1, v/v) for 20 min at 60 degrees C. The methylene unit values of the trimethylsilyl derivatives of these N-acylglycines are reported.
D002264:Carboxylic Acids / Q000032:analysis*; D002849:Chromatography, Gas; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D008722:Methods; D013329:Structure-Activity Relationship
D002264:Carboxylic Acids; D005998:Glycine