[Use of an ultramicroelectrophoretic method for separating blood serum cellular and isoenzymes].
Methods for staining of ultramicroenzymelectrophoregrams are described. Sharp and well reproducible bands of isozymes of LDH, MDH, G-6-PD, AcP, AlP, alpha-NAE were obtained from homogenates of cultivated human fibroblasts, of rat's liver, kidney and brain, of human embryonic liver, and in blood serum of man and frog. An extract from the rat brain containing only 3.4 ng of proteins or a quantity of 70-80 fibroblasts were sufficient for obtaining a well differentiated spectrum of LDH isozymes. The ultramicromethod significantly lowers the need in deficit reagents, reduces the cost and time of analysis.
D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001001:Anura; D001797:Blood Protein Electrophoresis / Q000379:methods; D001921:Brain / Q000166:cytology; D002477:Cells / Q000201:enzymology*; D002478:Cells, Cultured; D004586:Electrophoresis / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology; D004625:Embryo, Nonmammalian; D004950:Esterases / Q000032:analysis; D005347:Fibroblasts / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007668:Kidney / Q000166:cytology; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000166:cytology; D008291:Malate Dehydrogenase / Q000032:analysis; D008832:Microchemistry / Q000379:methods*; D009280:Naphthaleneacetic Acids / Q000032:analysis; D051381:Rats
D007527:Isoenzymes; D009280:Naphthaleneacetic Acids; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D004950:Esterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase
Primenenie metoda ul'tramikroélektroforeza dlina razdeleniia izofermentov syvorotki krovi i kletok