MCG101-AA, a new ascites tumour in C57 mice. 4. Influence of enzyme treatment on transplantability and some in vitro characteristics.
The transplantabilities of trypsin and neuraminidase-treated 101-AA cells were compared with untreated cells. The cell doses required for growth were similar for enzyme-treated and control cells. Yet, trypsinized cells injected i.v. gave rise to less lung tumours and, in one experiment, they produced more extrapulmonary tumours than control cells or neuraminidase-treated cells. These findings were collated with some data on the effect of enzyme treatment on the tumour cell membranes, obtained by particle size analysis, cell electrophoresis, lectin agglutinability and sialic acid determinations. The importance of cell surface characteristics for specific metastasis patterns, in this case a peculiar "myotrophism", is discussed.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D037102:Lectins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009439:Neuraminidase / Q000494:pharmacology*; D012794:Sialic Acids / Q000378:metabolism; D013268:Stimulation, Chemical; D014184:Transplantation, Homologous; D014357:Trypsin / Q000494:pharmacology*
D037102:Lectins; D012794:Sialic Acids; D009439:Neuraminidase; D014357:Trypsin