Comparative studies of the intestinal absorption of [3H]pteroylmonoglutamate and [3H]pteroylheptaglutamate in man.
Comparative efficiencies of absorption of crystalline folic acid polyglutamate and monoglutamyl folic acid were determined in 11 normal subjects by measurement of the excretion of radioisotope in the urine after oral administration of [3H]pteroylheptaglutamic acid ([3H]PteGlu7) synthesized in our laboratory and of [3H]pteroylglutamic acid ([3H]PGA). Following ingestion of 0.6 mumole of [3H]PteGlu7, urinary excretion of radioactivity over 48 hr averaged 56.1 +/- 11.2% of the total dose. By comparison the ingestion of 0.6 mumole of [3H]PGA resulted in an average urinary excretion of 70.8 +/- 13.0% for the same time period. Approximately 90% of the urinary radioactivity was excreted during the initial 24-hr collection period. The mean recovery of radioactivity in urine and stool was 94% and recovery exceeded 84% in all subjects. The principal radioactive compound in the urine chromatographed with standard pteroylmonoglutamates. By chromatography, urinary folates were monoglutamates whether [3H]PGA or [3H]PteGlu7 was administered. The time course of folate absorption for the study compounds was compared by measuring the rise in serum radioactivity after the oral folate dose. Peak values in serum folate radioactivity following [3H]PGA occurred at 1 hr, whereas the peak values after [3H]PteGlu7 more often occurred at 2 hr. Only monoglutamyl folate was detected in the serum. These studies demonstrate that in normal subjects physiological doses of crystalline monoglutamyl and crystalline heptaglutamyl folates are both absorbed with high degrees of efficiency.
D001681:Biological Assay; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D004586:Electrophoresis; D005243:Feces / Q000032:analysis; D005260:Female; D005492:Folic Acid / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007408:Intestinal Absorption; D008297:Male; D013997:Time Factors; D014316:Tritium / Q000032:analysis / Q000097:blood / Q000652:urine
D014316:Tritium; D005492:Folic Acid