Role of gene 2 in bacteriophage T7 DNA synthesis.
Studies have been carried out to elucidate the in vivo function of gene 2 in T7 DNA synthesis. In gene 2-infected cells the rate of incorporation of (3-H)thymidine into acid-insoluble material is about 60% that of cells infected with T7 wild type. Gene 2 mutants do not however produce viable phage after infection of the nonpermissive host. In T7 wild type-infected cells, a major portion of the newly alkaline sucrose gradients. The concatemers serve as precursors for the formation of mature T7 DNA as demonstrated in pulse-chase experiments. In similar studies carried out with gene 2-infected cells, concatemers are not detected when the intracellular DNA is analyzed at several different times during the infection process. The DNA made during a gene 2 infection is present as duplex structures with a sedimentation rate close to mature T7 DNA.
D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000254:growth & development / Q000378:metabolism*; D004261:DNA Replication; D004267:DNA Viruses / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D005796:Genes; D009154:Mutation; D009694:Nucleic Acid Precursors / Q000096:biosynthesis; D013997:Time Factors; D014779:Virus Replication
D004279:DNA, Viral; D009694:Nucleic Acid Precursors