Quantitation of tryptophan metabolites in rat feces by thin-layer chromatography.
Indole, skatole, indole-3-acetic acid, indole-3-propionic acid, tryptamine, and free tryptophan have been extracted from rat fecal samples and quantitated by thin-layer chromatography. Additional tryptophan metabolites have been recovered in yields of 87-97% from supplemented fecal samples. Detection limits for all the compounds studied were in the 0.1 mug/g feces range. Quantitation was possible in the mug/g range with an estimated accuracy of plus or minus 10%.
D000818:Animals; D002855:Chromatography, Thin Layer; D005243:Feces / Q000032:analysis*; D007210:Indoleacetic Acids / Q000032:analysis; D007211:Indoles / Q000032:analysis; D008722:Methods; D011422:Propionates / Q000032:analysis; D051381:Rats; D012862:Skatole / Q000032:analysis; D014363:Tryptamines / Q000032:analysis; D014364:Tryptophan / Q000032:analysis* / Q000378:metabolism
D007210:Indoleacetic Acids; D007211:Indoles; D011422:Propionates; D014363:Tryptamines; D014364:Tryptophan; D012862:Skatole