Separation of fresh tobacco smoke on a packed polar gas chromatographic column prior to on-line analysis by gas chromatography-mass spectrometry using a non-polar capillary column.
Gaseous samples of fresh tobacco smoke were injected on to a packed polar column (2,2-oxydipropionitrile) in all-glass chromatographic system. With the aid of a warm syringe, selected fractions were withdrawn from the bottom of the electron capture detector of the packed column for further injection into an efficient non-polar glass capillary column (SF-96), connectable on-line to a mass spectrometer. The method has permitted the separation and identification of some polar and non-polar components of tobacco smoke, which gave previously mixed, broad, tailing peaks when the smoke was injected directly into a non-polar capillary column.
D002849:Chromatography, Gas; D006358:Hot Temperature; D006838:Hydrocarbons / Q000032:analysis; D013058:Mass Spectrometry; D009862:Online Systems; D010947:Plants, Toxic; D012906:Smoke / Q000032:analysis*; D014026:Nicotiana
D006838:Hydrocarbons; D012906:Smoke