Mucinous secretion of totally isolated porcine stomach perfused with homologous blood.
Principal glycoproteins and amino acids were determined in porcine gastric juice obtained from totally isolated stomachs perfused extracorporeally with homologous blood. Acid juices collected during prolonged stimulation of stomachs with histamine or pentagastrin, infused into gastric arteries, were used. All samples showed the presence of glactose, glucosamine, galactosamine, fucose, mannose and sulphate. Galactose and glucosamine were found in equimolar amounts and the galactose to galactosamine ratio was, in most samples, 2:1. Compared with human non-stimulated gastric juice studied by others, marked differences were found in the relationship between carbohydrate components and between amino acids. Porcine gastric juice contained much more protein than human gastric juice. Some characteristics of porcine mucinous secretion may be due to conditions of juice collection from the isolated gastric preparation.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001769:Blood; D005112:Extracorporeal Circulation; D005260:Female; D005750:Gastric Juice / Q000378:metabolism*; D005752:Gastric Mucins / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D008297:Male; D010418:Pentagastrin / Q000494:pharmacology; D010477:Perfusion; D012636:Secretory Rate / Q000187:drug effects; D013045:Species Specificity; D013268:Stimulation, Chemical; D013270:Stomach / Q000502:physiology*; D013552:Swine
D000596:Amino Acids; D005752:Gastric Mucins; D006023:Glycoproteins; D006632:Histamine; D010418:Pentagastrin