Controlled pore glass chromatography of protein-sodium dodecyl sulphate complexes.
The inclusion of urea has been found to eliminate adsorption of protein-sodium dodecyl sulphate (SDS) complexes to controlled pore glass. Using buffer containing 6 M urea, 0.5% SDS and glass with pore diameter 12.3 nm, it is possible to determine protein molecular weights in the range 3500-12,000. Results with glass of larger pore diameter (25.5 nm) are similar to those reported in the absence of urea in the molecular-weight range 12,000-140,000. Controlled pore glass chromatography also permits the study of the relative importance of conformation free of charge effects for those proteins which deviate from the normal calibration curve for SDS-polyacrylamide gels.
D000327:Adsorption; D000818:Animals; D002021:Buffers; D002850:Chromatography, Gel / Q000379:methods*; D005898:Glass; D008970:Molecular Weight; D011489:Protein Denaturation; D011506:Proteins; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate; D014508:Urea
D002021:Buffers; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D014508:Urea