Big growth hormone (GH): conversion to small GH without peptide bond cleavage.
Mercaptoethanol treatment of big growth hormone (GH) obtained from human pituitary resulted in a 60% conversion to small GH. Further dissociation was obtained by combined treatment with the reducing agent plus urea. The results indicate that the existence of big GH is dependent upon the formation of inter-polypeptide chain disulfide bonds.
D001665:Binding Sites; D002850:Chromatography, Gel; D004220:Disulfides; D004355:Drug Stability; D013006:Growth Hormone / Q000276:immunology; D006801:Humans; D046911:Macromolecular Substances; D008623:Mercaptoethanol; D008970:Molecular Weight; D010902:Pituitary Gland / Q000032:analysis; D011485:Protein Binding; D011863:Radioimmunoassay; D014508:Urea
D004220:Disulfides; D046911:Macromolecular Substances; D008623:Mercaptoethanol; D014508:Urea; D013006:Growth Hormone