Automated elution electrophoresis: a potential clinical tool.
An elution electrophoresis system in which a porous packed bed is used for separation and a flow photometer or colorimeter for continuous monitoring of the eluate may be capable of rapid, high-resolution analysis of serum proteins and other protein mixtures with very little manual labor. In a prototype of such a system we used a cooled separations column, 3 mm in diameter and 40 cm long, containing polyacrylamide beads. Samples are introduced, via a microsyringe, through a septum at the column midpoint. Typical analyses for serum proteins or serum isoenzymes require an electrophoresis time of about 30 min at 1200 V.
D001322:Autoanalysis; D001798:Blood Proteins / Q000302:isolation & purification*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate; D005069:Evaluation Studies as Topic; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood; D013056:Spectrophotometry, Ultraviolet
D001798:Blood Proteins; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase