Purification and characterization of 2-alkenal reductase.
The purification of a 2-alkenal reductase to homogeneity from a rat liver 100 000 times g supernatant is described. Its molecular weight has been determined by Sephadex G-100 chromatography and sodium dodecylsulfate polyacrylamide gel electrophoresis before and after reduction with mercaptoethanol and carboxymethylation. The monometric form has a molecular weight of 45 000. It tends to form, to a very small extent, dimeric and trimeric aggregates of molecular weights 90 000 and 135 000. The isoelectric point (IP) was determined to be 6.2 by isoelectric focusing.
D000475:Alkenes; D000818:Animals; D002458:Cell Fractionation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000201:enzymology; D004589:Electrophoresis, Disc; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D008099:Liver / Q000201:enzymology; D008623:Mercaptoethanol; D008970:Molecular Weight; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification*; D011489:Protein Denaturation; D051381:Rats
D000475:Alkenes; D008623:Mercaptoethanol; D009247:NADH, NADPH Oxidoreductases