Protein synthesis in chloroplasts. Characteristics and products of protein synthesis in vitro in etioplasts and developing chloroplasts from pea leaves.
The function of plastid ribosomes in pea (Pisum sativum L.) was investigated by characterizing the products of protein synthesis in vitro in plastids isolated at different stages during the transition from etioplast to chloroplast. Etioplasts and plastids isolated after 24, 48 and 96h of greening in continuous white light, use added ATP to incorporate labelled amino acids into protein. Plastids isolated from greening leaves can also use light as the source of energy for protein synthesis. The labelled polypeptides synthesized in isolated plastids were analysed by electrophoresis in sodium dodecyl sulphate-ureapolyacrylamide gels. Six polypeptides are synthesized in etioplasts with ATP as energy source. Only one of these polypeptides is present in a 150 000g supernatant fraction. This polypeptide has been identified as the large subunit of Fraction I protein (3-phospho-D-glycerate carboxylyase EC 4.1.1.39) by comparing the tryptic 'map' of its L-(35S)methionine-labelled peptides with the tryptic 'map' of large subunit peptides from Fraction I labelled with L-(35S)methionine in vivo. The same gel pattern of six polypeptides is seen when plastids isolated from greening leaves are incubated with either added ATP or light as the energy source. However, the rates of synthesis of particular polypeptides are different in plastids isolated at different stages of the etioplast to chloroplast transition. The results support the idea that plastid ribosomes synthesize only a small number of proteins, and that the number and molecular weight of these proteins does not alter during the formation of chloroplasts from etioplasts.
D000255:Adenosine Triphosphate; D000367:Age Factors; D000596:Amino Acids / Q000378:metabolism; D002262:Carboxy-Lyases / Q000032:analysis; D002736:Chloroplasts / Q000032:analysis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D010940:Plant Proteins / Q000096:biosynthesis*; D010944:Plants / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate
D000596:Amino Acids; D010455:Peptides; D010940:Plant Proteins; D012967:Sodium Dodecyl Sulfate; D000255:Adenosine Triphosphate; D002262:Carboxy-Lyases