Neutral glycosphingolipids containing mannose from the bivalve Corbicula sandai.
1. A unique subclass of ceramide oligosaccharides from whole tissue of the fresh-water bivalve Corbicula sandai has been isolated. Through the use of chemical, enzymatic, and physical techniques, two novel glycolipids were characterized as mannosyl-beta (1 leads to 4)-glucosyl ceramide and mannosyl-alpha (1 leads to 4)-mannosyl-beta (1 leads to 4)-glucosyl ceramide. 2. The components of fatty acids and long chain bases in the two glycolipids were analyzed by gas-liquid chromatography and were further identified by mass spectrometry. The data show that, with respect to the major components, both lipids have similar caramide moieties.
D000818:Animals; D002518:Ceramides / Q000032:analysis; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D006028:Glycosphingolipids / Q000302:isolation & purification; D006601:Hexoses / Q000032:analysis; D007668:Kidney / Q000201:enzymology; D008358:Mannose / Q000032:analysis*; D008361:Mannosidases; D008974:Mollusca / Q000032:analysis*; D012908:Snails / Q000201:enzymology; D013110:Sphingosine / Q000032:analysis; D013552:Swine
D002518:Ceramides; D005227:Fatty Acids; D006028:Glycosphingolipids; D006601:Hexoses; D008361:Mannosidases; D013110:Sphingosine; D008358:Mannose