Effects of lysophosphatidyl choline and phospholipase A on the lens.
Lenses incubated for 24 or 63 hours in media containing either lysophosphatidyl choline or phospholipase A gained Na+ ions and water. Electrolyte imbalances and damage to lens fiber membranes occurred at lysophosphatidyl choline and phospholipase A concentrations of 7.5 mug per milliliter and 0.25 mug per milliliter, respectively. Intravitreal injection of 250 mug of lysophosphatidyl choline or 0.4 mug of phospholipase A induced posterior subcapsular cataracts which progressed to maturity only in the latter instance. This is the first demonstration of the cataractogenic effects of a naturally occurring aqueous humor phospholipid or its generating enzyme.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D010713:Phosphatidylcholines / Q000494:pharmacology*; D010740:Phospholipases / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D011817:Rabbits; D012964:Sodium / Q000378:metabolism; D014617:Vacuoles / Q000648:ultrastructure; D014867:Water / Q000378:metabolism
D010713:Phosphatidylcholines; D014867:Water; D012964:Sodium; D010740:Phospholipases; D011188:Potassium