The effect of a synthetic double-stranded RNA on IgC1 and IgE production by guinea-pigs. A comparative study with lipopolysaccharide.
The effect of synthetic complexes of polyribocytidylic acid (poly-IC) on the production of homocytotropic antibodies by guinea-pigs was studied. It was observed that poly-IC has a definite adjuvant effect on the production of both IgG1 and IgE. The adjuvant effect was present when poly-IC was injected 24 hours after the antigen, but was absent when the polynucleotide was injected 48 hours after the antigen. In comparison with lipopolysaccharide (LPS) the adjuvant effect of poly-IC was weaker, more than 0.5 mg/kg of this polynucleotide being required to show an adjuvant effect, whereas only 20 mug/kg of LPS was enough to show a positive adjuvant effect. Furthermore, considerably less antibody was detected in the animals stimulated with the polynucleotide.
D000276:Adjuvants, Immunologic; D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D005260:Female; D006168:Guinea Pigs; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D011070:Poly I-C / Q000276:immunology*; D013997:Time Factors
D000276:Adjuvants, Immunologic; D000941:Antigens; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D007073:Immunoglobulin E; D010047:Ovalbumin; D011070:Poly I-C