Biochemical genetics of alpha-amylase isozymes of the chicken pancreas.
In the chicken population at large, three electrophoretically distinct pancreatic alpha-amylase isozymes were discovered. The isozymes were designated Pa 1, Pa 2, and Pa 3. The local population of chickens, however, possessed only isozymes Pa 2 and Pa 3 present as three phenotypes: Amy-2 B, consisting of isozyme Pa2; Amy2 BC, consisting of isozymes Pa 2 plus Pa 3; and Amy2 C, consisting of isozyme Pa 3. Pancreatic biopsy permitted the establishment of a breeding flock with defined amylase phenotypes. Matings of this flock established that amylases are inherited as codominant alleles at a single genetic locus. Further, there was no evidence of ontogenetic modification of the amylase isozymes. It was observed that amylase isozymes Pa 2 and Pa 3 each generated a family of at least three faster-migrating amylolytic proteins. These post-translationally modified amylases were designated Pa Xa, Pa Xb, and Pa Xc, where X represents the number of the progenitor amylase. Structural analyses of purified amylases demonstrated that all amylase isozymes are nonglycosidated, monomeric molecules of molecular weight 55,000. In addition, the data are consistent with the hypothesis that the faster-migrating amylases are produced by deamidation of asparagine and/or glutamine residues.
D000483:Alleles; D000596:Amino Acids / Q000032:analysis; D000681:Amylases / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002645:Chickens; D002874:Chromosome Mapping; D004591:Electrophoresis, Polyacrylamide Gel; D005799:Genes, Dominant; D014644:Genetic Variation; D007527:Isoenzymes / Q000378:metabolism*; D008967:Molecular Biology; D008970:Molecular Weight; D010179:Pancreas / Q000201:enzymology*; D010455:Peptides / Q000032:analysis; D010641:Phenotype; D014176:Protein Biosynthesis; D012967:Sodium Dodecyl Sulfate
D000596:Amino Acids; D002241:Carbohydrates; D007527:Isoenzymes; D010455:Peptides; D012967:Sodium Dodecyl Sulfate; D000681:Amylases