[Mechanism of the enzymatic reaction catalyzed by uridine diphosphate glucose dehydrogenase. The chemical synthesis of uridine diphosphate glucose-6-H3 and its oxidation by uridine diphosphate glucose dehydrogenase].
The synthesis of UDP-glucose-6-s-H was performed through condensation of alpha-D-glucopyranosyl phosphate-6-3-H and uridine 5'-phosphomorpholidate. Enzymic oxidation of UDP-glucose-6-3-H with calf liver UDP-glucose dehydrogenase was found to proceed with direct transfer of the hydrogen from C-6 of UDP-glucose onto NAD.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D002417:Cattle; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D008099:Liver / Q000201:enzymology; D010084:Oxidation-Reduction; D012588:Scintillation Counting; D013053:Spectrophotometry; D014316:Tritium; D014532:Uridine Diphosphate Glucose / Q000138:chemical synthesis*; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D014539:Uridine Diphosphate Sugars / Q000138:chemical synthesis*
D014539:Uridine Diphosphate Sugars; D014316:Tritium; D000429:Alcohol Oxidoreductases; D014533:Uridine Diphosphate Glucose Dehydrogenase; D014532:Uridine Diphosphate Glucose
O mekhanizme fermentativnoĭ reaktsii, kataliziruemoĭ uridindifosfatgliukoza-degidrogenazoĭ. Khimicheskiĭ sintez uridindifosfatgliukozy-6-H3 i ee okislenie uridindifosfatgliukoza-degidrogenazoĭ