p-CMB-dextran T 10. A useful tool in evaluating the functional importance of superficial SH-groups in rat adipocytes.
A large SH-blocking reagent p-CMB-dextran T 10 (MW about 10,000) was applied to isolated rat adipocytes. This compound possesses the same reactivity towards SH-groups as uncoupled p-CMB. P-CMB-Dextran T 10 did not influence the basal glucose uptake in contrast to uncoupled p-CMB. The results are discussed with respect to the usefulness of p-CMB-dextran T 10 in evaluating the role of superficial SH-groups in various membrane functions.
D000273:Adipose Tissue / Q000032:analysis* / Q000187:drug effects; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002729:Chloromercuribenzoates / Q000494:pharmacology*; D003911:Dextrans / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
D002729:Chloromercuribenzoates; D003911:Dextrans; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D014280:Triglycerides; D002245:Carbon Dioxide; D005947:Glucose