The use of ammonium sulphate precipitation method for the determination of antigen-binding capacity and affinity of anti-tetanus antibodies in human serum.
Radioimmunoassay of anti-tetanus antibodies produced in human serum in response to tetanus toxoid immunization was carried out by employing the non-specific precipitation effect of ammonium sulphate described by Farr (1958). The results showed that the method was sensitive and was able to differentiate between immune and non-immune persons. The effects of booster dose injection on the quantity and quality of antibodies produced during immunization were discussed, and the possible use of the method to predict the amount of immunogen, the timing and number of injections required for optimal host response in immunization schedules was suggested.
D000645:Ammonium Sulfate; D000937:Antigen-Antibody Reactions; D011232:Chemical Precipitation; D005260:Female; D006801:Humans; D007106:Immune Sera; D007115:Immunization Schedule; D007117:Immunization, Secondary; D007457:Iodine Radioisotopes; D008297:Male; D011863:Radioimmunoassay / Q000379:methods*; D013742:Tetanus / Q000276:immunology*; D013743:Tetanus Antitoxin; D013745:Tetanus Toxoid / Q000627:therapeutic use
D007106:Immune Sera; D007457:Iodine Radioisotopes; D013743:Tetanus Antitoxin; D013745:Tetanus Toxoid; D000645:Ammonium Sulfate