A method for testing the effects of fluids on rat skin using acrylic chambers.
A method is presented whereby test fluids may be kept in contact with the skin of the back of the rat for 5 to 6 days. Methyl methacrylate chambers with hemispherical test domes were made and attached to rubber washers of larger diameter, which in turn were attached to the skin of the back of the rat using an alkyl cyanoacrylate (Eastman 910 adhesive). Each chamber had a removable stainless-steel screw to allow it to be filled and replenished with fluid as required. To prevent the rat gnawing the chambers from its back, twice weekly 4 mm was removed from the lower incisors and 1 1/2 mm from the upper incisors, using a diamond disc in a dental handpiece. The young adult rats were fed a soft diet and after an initial temporary fall in weight, they moved about the cages in a normal manner.
D000287:Administration, Topical; D000818:Animals; D001835:Body Weight; D003487:Cyanoacrylates; D004032:Diet; D008297:Male; D008722:Methods; D008768:Methylmethacrylates; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*; D051381:Rats; D012867:Skin / Q000187:drug effects*; D012996:Solutions
D003487:Cyanoacrylates; D008768:Methylmethacrylates; D004364:Pharmaceutical Preparations; D012996:Solutions