A micro method for simultaneous determination of galactosyltransferase and 5'-nucleotidase activities in cell fractions.
Galactosyltransferase and 5'-nucleotidase were assayed in the same reaction mixture, with ovalbumin as exogenous acceptor of (14-C)galactose and with (3-H)AMP as the substrate for the 5'-nucleotidase assay. The substrates and reaction products of either assay had no significant effect on the activity of the other enzyme.
D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D005690:Galactose / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008722:Methods; D009708:Nucleotidases / Q000378:metabolism*; D010047:Ovalbumin; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology*
D000249:Adenosine Monophosphate; D010047:Ovalbumin; D006602:Hexosyltransferases; D009708:Nucleotidases; D005690:Galactose