[Influence of thyroid status on the electrophoretic pattern of rat liver mitochondrial proteins].
Liver mitochondria were isolated from normal and thyroidectomized rats and their protein components analyzed by polyacrylamide gel electrophoresis. In whole mitochondria 35 protein fractions with MW ranging from 10,000 to 135,000 were characterized. In the absence of thyroid hormone secretion, the amount of a MW 54,000 fraction was always decreased. Injection of small doses of 3,5,3'-triiodo-L-thyronine to the thyroidectomized animal restored the quantity of that protein fraction to normal. Isolated outer mitochondrial membranes showed the presence of 20 protein fractions. These fractions revealed no change after thyroidectomy. The mitoplast, which contained 35 fractions, exhibited a decrease of the MW 54,000 component in thyroidectomized rats. The mitoplast was separated into several fractions. Water soluble matrix proteins presented molecular weights ranging between 40,000 and 55,000. Proteins, which were slightly bound to the inner mitochondrial membrane and could be extracted by KCl, presented molecular weights between 25,000 and 45,000. Structural proteins showed a principal specific component of MW equals 23,000. Electrophoretic patterns obtained with these submitochondrial fractions were similar in normal and thyroidectomized animals. The mitoplast fraction which contained the insoluble cytochromes (a, a3, b, c1) was isolated ; its principal constituent, of MW 54,000 was significantly decreased after thyroidectomy. Thus, the lack of thyroid hormone secretion lowered the level of a protein constituent bound to the inner membrane of liver mitochondria. The synthesis of this constituent could be controlled by mitochondrial nucleic acids.
D000818:Animals; D003580:Cytochromes / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008566:Membranes; D008930:Mitochondria, Liver / Q000032:analysis / Q000187:drug effects*; D008970:Molecular Weight; D011485:Protein Binding; D011506:Proteins / Q000032:analysis; D051381:Rats; D013965:Thyroidectomy; D014284:Triiodothyronine / Q000494:pharmacology*
D003580:Cytochromes; D011506:Proteins; D014284:Triiodothyronine
Influence de l'état thyroïdien sur le profil électrophorétique des protéines mitochondriales de foie de rat