[Variability of the levorin producer, Actinomyces levoris Krass].
Natural variation of the levorin-producing organism Act. levoris, strain 28 was studied with respect to the colony morphology and production of levorin and levoristatin. The population of strain 28 consisted of 3 morphological colony types, the main type amounting to 99.7 percent. The strain variation with respect to production of levorin and levoristatin ranged from 20 to 180 and from 0 to 300 percent respectively as compared to the control. Mutant M-28 differing from the initial strain by the colony morphology, moderate phage titer and preferable production of levoristatin was isolated as a result of repeated passages of strain 28 onto agarized Chapek media with starch without maintaining selection. Variants differing from the population of strains 28 and M-28 by the ratio of levorin and levoristatin in the culture fluid were selected. No correlation in production of the above antibiotics by strain 28 was noted. Preparations obtained with strain M-28 differed from those obtained with strain 28 in a significant content of levoristatin.
D000190:Actinomyces / Q000166:cytology / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000032:analysis; D000935:Antifungal Agents / Q000096:biosynthesis / Q000302:isolation & purification*; D002854:Chromatography, Paper; D003377:Countercurrent Distribution; D003470:Culture Media; D003609:Dactinomycin / Q000031:analogs & derivatives; D014644:Genetic Variation; D009154:Mutation; D011090:Polyenes / Q000302:isolation & purification / Q000378:metabolism; D012641:Selection, Genetic; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003470:Culture Media; D011090:Polyenes; C008276:levoristatin; D003609:Dactinomycin
Izmenchivost' produtsenta levorina--Actinomyces levoris Krass