The quantitative protein composition of calf thymus chromatin.
The proteins of calf thymus chromatin were analysed quantitatively using a combination of polyacrylamide and cellogel electrophoreses. Quantification was achieved by determining the staining values of each purified histone with amido-black. The results indicate that, on average, 700 molecules histone F1, 850 of F2al, 1440 of F2a2, 1 890 of F2b, 2380 of F3 and 500-1000 non-histone molecules are bound per 10(5) base pairs of DNA. This suggests a moderately dense protein covering of the DNA.
D000580:Amido Black; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000032:analysis; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis*; D013950:Thymus Gland / Q000032:analysis / Q000648:ultrastructure*
D000596:Amino Acids; D002843:Chromatin; D006657:Histones; D009698:Nucleoproteins; D011506:Proteins; D004247:DNA; D000580:Amido Black