Improved acetylating radioenzymatic assay of amikacin, tobramycin, and sisomicin in serum.
The acetylating radioenzymatic assay of aminoglycosides was modified by using [(3)H]acetyl coenzyme A as cosubstrate. This modification reduces the cost of the method by at least one-half without sacrificing rapidity or accuracy.
D000583:Amikacin / Q000097:blood*; D000900:Anti-Bacterial Agents / Q000097:blood*; D003065:Coenzyme A; D007612:Kanamycin / Q000031:analogs & derivatives*; D008722:Methods; D012853:Sisomicin / Q000097:blood*; D014031:Tobramycin / Q000097:blood*
D000900:Anti-Bacterial Agents; D007612:Kanamycin; D000583:Amikacin; D003065:Coenzyme A; D014031:Tobramycin; D012853:Sisomicin