An artifact in the gas chromatographic analysis of urinary organic acids from phenylketonuric children: decarboxylation of the phenylpyruvic acid during extraction.
Phenylpyruvic acid is converted at least partially to phenylacetic acid during the normal extraction procedure used to obtain urinary organic acid profiles of phenylketonuric children by gas chromatography. This decarboxylation reaction can be reduced or completely eliminated by forming oxime derivatives prior to extraction.
D002648:Child; D002849:Chromatography, Gas / Q000379:methods*; D003653:Decarboxylation; D005188:False Negative Reactions; D006801:Humans; D013058:Mass Spectrometry; D010091:Oximes; D010661:Phenylketonurias / Q000175:diagnosis*; D010667:Phenylpyruvic Acids / Q000652:urine*
D010091:Oximes; D010667:Phenylpyruvic Acids