On the character of the nuclear membrane-associated DNA of HeLa cells.
The nature of the nascent DNA-membrane complexes isolated from synchronized HeLa cells was examined. It was found that membrane-associated DNA is not the result of repair replication. A study of the stability of the complexes to various degradative agents and enzymes suggest that the DNA is associated with a structure which is composed of a lipoprotein. An examination of the physical structure of this DNA indicates that it might be a "nicked" duplex with a base content similar to that of the bulk DNA.
D001665:Binding Sites; D002250:Carbon Radioisotopes; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism*; D002500:Centrifugation, Isopycnic; D002794:Choline / Q000378:metabolism; D002845:Chromatography; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair; D003840:Deoxycholic Acid / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000494:pharmacology; D005472:Fluorouracil / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008566:Membranes / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D010447:Peptide Hydrolases / Q000494:pharmacology; D010740:Phospholipases / Q000494:pharmacology; D012260:Ribonucleases / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D002250:Carbon Radioisotopes; D003840:Deoxycholic Acid; D012965:Sodium Chloride; D004247:DNA; D003851:Deoxyribonucleases; D010740:Phospholipases; D012260:Ribonucleases; D010447:Peptide Hydrolases; D002794:Choline; D005472:Fluorouracil; D013936:Thymidine