A new method for determining liver microsomal cholesterol 7alpha-hydroxylase.
A method is described to determine the mass of 7alpha-hydroxy cholesterol synthetized in vitro by liver microsomes without the use of a radioactive substrate.
D000818:Animals; D001894:Borohydrides; D002784:Cholesterol / Q000031:analogs & derivatives; D002790:Cholesterol 7-alpha-Hydroxylase / Q000032:analysis*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005260:Female; D006888:Hydroxycholesterols / Q000032:analysis / Q000138:chemical synthesis; D007553:Isotope Labeling; D007664:Ketosteroids; D007700:Kinetics; D008297:Male; D008722:Methods; D008862:Microsomes, Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D051381:Rats; D013250:Steroid Hydroxylases / Q000032:analysis*; D014316:Tritium
D001894:Borohydrides; D006888:Hydroxycholesterols; D007664:Ketosteroids; D014316:Tritium; D002784:Cholesterol; D013250:Steroid Hydroxylases; D002790:Cholesterol 7-alpha-Hydroxylase