Papain-solubilized Ag-B antigens. I. Isolated and characterization of two components composing Ag-B antigens.
Ag-B antigen molecules of about 59,000 daltons were partially purified from papain digests of liver cell membranes of Fischer and ACI rats. These preparations were radioiodinated and the labeled Ag-B antigen molecultes were isolated as specific immune complexes with alloantibodies directed to Ag-B1 or Ag-B4. These specifically purified Ag-B antigen molecules were found to give two fragments of 37,000 and 11,000 daltons on sodium sulfate-acrylamide gel electrophoresis. The two fragments (or very similar ones) were isolated from the radioiodinated partially purified Ag-B antigen preparations by acid dissociation and subsequent gel filtration. The 37,000-dalton fragment retained the same Ag-B alloantigenic specificity as the parental 59,000-dalton Ag-B antigen molecules, whereas the 11,000-dalton fragment did not carry any detectable Ag-B alloantigenic activity. In the reaction with rabbit antisera raised against rat cell membranes, each fragment was shown to be antigenically distinctive.
D000818:Animals; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D002462:Cell Membrane / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D006649:Histocompatibility Antigens / Q000302:isolation & purification*; D007106:Immune Sera; D007457:Iodine Radioisotopes; D007518:Isoantibodies; D008099:Liver / Q000166:cytology; D010206:Papain; D011863:Radioimmunoassay; D051381:Rats / Q000276:immunology*; D011919:Rats, Inbred Strains; D012967:Sodium Dodecyl Sulfate
D000936:Antigen-Antibody Complex; D006649:Histocompatibility Antigens; D007106:Immune Sera; D007457:Iodine Radioisotopes; D007518:Isoantibodies; D012967:Sodium Dodecyl Sulfate; D010206:Papain