Assay of flucytosine and furosemide by high-pressure liquid chromatography.
A high-pressure liquid chromatographic method for the analysis of flucytosine and furosemide concentrations in biological fluid is described. The separations were carried out on a pellicular cation-exchange resin eluted with an ammonium phosphate buffer. Detection of elution peaks was by UV absorption at 280 nm and fluorescence monitoring. Advantages of the method are specificity, minimal preanalysis sample workup, and small sample size.
D002021:Buffers; D002852:Chromatography, Ion Exchange; D003596:Cytosine / Q000031:analogs & derivatives*; D005437:Flucytosine / Q000032:analysis*; D005665:Furosemide / Q000032:analysis*; D008722:Methods; D013056:Spectrophotometry, Ultraviolet; D013462:Sulfur Radioisotopes
D002021:Buffers; D013462:Sulfur Radioisotopes; D005665:Furosemide; D003596:Cytosine; D005437:Flucytosine