Effect of UV-irradiation in vitro on adenin nucleotides metabolism, Na+ and K+ concentration, osmotic properties and submicroscopic structure of pigeon red blood cells.
Effect of UV-irradiation in vitro on metabolism of adenine nucleotides: ADP, ATP and AXP, osmotic properties and submicroscopic structure of nucleated pigeon red blood cell was investigated. Irradiation carried out for 60, 120, 180, 240 and 300 minutes. Hanau S-500 lamp with efficiency 4.34-10(8) erh/sec was used. A decrease of ATP content with a stimultaneous increase of ADP and AXP contents and a rather constant level of the sum of adenine compounds was observed. UV-irradiation caused a decrease of reversal of hemolysis and osmotic resistance to hypotonic NaCl solutions. An equivalent exchange of Na+ and K+ ion and an increase of hematocrit value, following UV-irradiation was observed. Electron microscope studies demonstrated changes of ultrastructure concerning both cell nucleus and thickness and granulation of cell membrane.
D000227:Adenine Nucleotides / Q000097:blood*; D000244:Adenosine Diphosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D010856:Columbidae; D004912:Erythrocytes / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D006461:Hemolysis; D007038:Hypotonic Solutions; D008854:Microscopy, Electron; D009996:Osmotic Fragility / Q000528:radiation effects; D011188:Potassium / Q000097:blood*; D011830:Radiation Effects; D012964:Sodium / Q000097:blood*; D013053:Spectrophotometry; D014466:Ultraviolet Rays
D000227:Adenine Nucleotides; D007038:Hypotonic Solutions; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D012964:Sodium; D011188:Potassium