The protein nature of the Thy-1.2 alloantigen as expressed by the murin lymphoblastoid line S-49.1 TB-2-3.
This preliminary study was undertaken to investigate the chemical nature of the Thy-1.2 antigen expressed on the murine cell line S-49.1 TB-2-3 (S-49). The presence of the Thy-1.2 antigen was indicated by the inhibition of AKR anti-C3-Thy-1.2 serum induced lysis of 51Cr-primed target cells. It was found that limited digestion of S-49 cells with crude papain yielded a Thy-1.2-containing solution. The protein nature of the Thy-1.2 antigen obtained in this manner was indicated by changes after proteolytic digestion. Separate digestions with crystalline papain, insolubilized papain, and insolubilized protease all destroyed the Thy-1.2 activity. These results suggest that the protein moiety is necessary for Thy-1.2 activity.
D000042:Absorption; D000818:Animals; D000918:Antibody Specificity; D002460:Cell Line; D002725:Chloroform; D002850:Chromatography, Gel; D002860:Chromium Radioisotopes; D003165:Complement System Proteins; D003460:Crystallization; D003601:Cytotoxicity Tests, Immunologic; D006168:Guinea Pigs / Q000276:immunology; D007106:Immune Sera; D007519:Isoantigens / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology*; D000432:Methanol; D051379:Mice; D008806:Mice, Inbred AKR; D008970:Molecular Weight; D010206:Papain; D010447:Peptide Hydrolases; D011506:Proteins / Q000032:analysis*; D012995:Solubility; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*
D002860:Chromium Radioisotopes; D007106:Immune Sera; D007519:Isoantigens; D011506:Proteins; D002725:Chloroform; D003165:Complement System Proteins; D010447:Peptide Hydrolases; D010206:Papain; D000432:Methanol