Ultrastructural detection of lectin receptors by cytochemical affinity reaction using mannan-iron complex.
A two-step affinity reaction is described for electron microscopic demonstration of the Concanavalin A as well as the Lens culinaris lectin receptors by means of the yeast mannan-iron complex. First the tissue was incubated in the lectin. Afterwards the incubation in the yeast mannan-iron complex was performed and reaction takes place between the still free second sugar binding site of membrane bound lectin molecules and the polysaccharides. This membrane receptor-lectin-polysaccharide complex is revealed by the electron dense iron core of the yeast mannan-iron complex. The specificity of the reactions could be demonstrated by addition of the hapten or by incubation in the yeast mannan-iron complex only. The proposed technique has proved useful for demonstration of lectin receptors in the small intestine.
D000818:Animals; D001665:Binding Sites; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000032:analysis; D003208:Concanavalin A / Q000032:analysis; D007421:Intestine, Small / Q000032:analysis; D007501:Iron; D037102:Lectins / Q000032:analysis*; D008358:Mannose; D051379:Mice; D008854:Microscopy, Electron; D015003:Yeasts
D037102:Lectins; D003208:Concanavalin A; D007501:Iron; D008358:Mannose