[Substrate specifity of "cholesteroloxidase" for the enzymatic determination of cholesterol].
The substrate specifity of "Cholesteroloxidase" (E.C.1.1.3.6) from Nocardia erythropolis was studied. The main sterols from animal and plant fats and 4-methylsterols as well as one triterpene served as substrates. The enzyme is not specific for cholesterol but also reacts with all the natural 3 beta-hydroxysterols. However, the enzymatic reaction may contribute as a new analytical method to determine the "total sterols" in the unsaponifiable matter of fats.
D000429:Alcohol Oxidoreductases; D002021:Buffers; D002784:Cholesterol / Q000032:analysis*; D009615:Nocardia / Q000201:enzymology; D010084:Oxidation-Reduction; D010783:Photometry; D010840:Phytosterols
D002021:Buffers; D010840:Phytosterols; D002784:Cholesterol; D000429:Alcohol Oxidoreductases
Zur Spezifität der "Cholesterinoxydase" für die enzymatische Cholesterinbestimmung