The preparation and properties of factor V from bovine plasma.
1. A procedure for the purification of factor V from bovine plasma with a good yield and in a high state of purity is described. The isolation procedure involves BaSO4, adsorption, chromatography of the absorbed plasma on DEAE-Sephadex A-50, activation with thrombin of the A-50 eluate, chromatography on Amberlite CG-50 and precipitation with (NH4)2SO4. Factor V is obtained with a 160% yield and 5000-fold purification. 2. Some of the physico-chemical properties of Ac-globulin have been measured. The molecular weight was determined by electrophoresis is SDS-polyacrylamide gel and found to be 30 000. The studies employing disc electrophoresis and immunoelectrophoresis suggest the existence of several active isoforms of factor V.
D000818:Animals; D001464:Barium; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D005165:Factor V / Q000302:isolation & purification; D007122:Immunoelectrophoresis; D007527:Isoenzymes / Q000302:isolation & purification; D008722:Methods; D008970:Molecular Weight; D011817:Rabbits / Q000276:immunology; D013917:Thrombin / Q000302:isolation & purification; D014461:Ultracentrifugation
D007527:Isoenzymes; D001464:Barium; D005165:Factor V; D013917:Thrombin