A new type of carbohydrate-protein linkage in a glycopeptide from normal human urine.
A glycopeptide, 3-O-beta-D-glucopyranosyl-alpha-L-fucopyranosyl-L-threonine, has been isolated from normal human urine. The glycopeptide was isolated by gel chromatography, preparative zone electrophoresis, paper chromatography, and high voltage electrophoresis. The average yield of the glycopeptide was in the range of 0.2 to 0.3 mg/liter of urine. Sugar analysis and amino acid analysis gave equimolar amounts of glucose, fucose, and threonine. Linkages and sequential order were established by methylation analysis of the glycopeptide after degradation of the amino acid residue with ninhydrin. The permethylated product was analyzed on gas liquid chromatography and mass spectrometry. Anomeric configuration was deduced from optical rotation.
D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D004586:Electrophoresis; D005643:Fucose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006020:Glycopeptides / Q000652:urine*; D006801:Humans; D013058:Mass Spectrometry; D011048:Polarography; D013912:Threonine / Q000032:analysis
D006020:Glycopeptides; D005643:Fucose; D013912:Threonine; D005947:Glucose