Isolation and characterization of the DNA fraction of rat liver chromatin which binds polylysine.
The structure of eukaryotic chromatin has been investigated by isolating and analyzing the "accessible" DNA fraction of rat liver chromatin. This DNA fraction has been isolated by titrating the chromatin with the protese-resistant D isomer of polylysine to bind the "accessible" DNA sites. After removal of chromosomal proteins by digestion with pronase, all DNA not protected from attack by bound polylysine was removed by digestion with DNase. Even after exhaustive treatment with pronase and DNase approximately 30% of the chromatin DNA remains resistant to nuclease attack. Analysis of the isolated DNA shows it to be mainly double-stranded with an average size of 200-250 base pairs. The DNA is slightly A-T rich and contains both repetitive and "single-copy" nuleotide sequences. The results suggest that there are extensive regions in chromatin where the DNA is not tightly complexed with protein. Furthermore, the DNA of these regions is similar in gross properties to the DNA of the total genome.
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000032:analysis*; D008297:Male; D009690:Nucleic Acid Conformation; D011132:Polyribosomes / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D013237:Stereoisomerism
D002843:Chromatin; D004247:DNA