Interference with the transport of heparin-releasable lipoprotein lipase in the perfused rat heart by colchicine and vinblastine.
The effect of pretreatment with colchicine or vinblastine on the lipoprotein lipase activity of rat heart was studied. Administration of colchicine or vinblastine 4 h prior to perfusion of the heart caused a very marked reduction in lipoprotein lipase activity released into the perfusate within 1 min of heparin perfusion. At the same time an increase in residual heart lipase occurred so that total lipoprotein lipase content of the heart (heparin releasable plus residual) did not change. The colchicine effect was dose and time dependent; no decrease in heparin-releasable enzyme activity occurred after only 30 min of pretreatment or upon addition of colchicine into the perfusate. These results indicate that colchicine did not impede enzyme synthesis or its release from the cell surface, but may have interfered with the transport of lipoprotein lipase from the site of its synthesis to the endothelial cell surface.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology*; D005260:Female; D006321:Heart / Q000187:drug effects*; D006493:Heparin / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology*; D010477:Perfusion; D011479:Protamines / Q000494:pharmacology; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D013347:Subcellular Fractions / Q000201:enzymology; D013997:Time Factors; D014747:Vinblastine / Q000494:pharmacology*
D011479:Protamines; D012965:Sodium Chloride; D014747:Vinblastine; D006493:Heparin; D008071:Lipoprotein Lipase; D003078:Colchicine