Column chromatographic analysis of barbiturates in their dosage forms. II. secobarbital, amobarbital, and pentobarbital.
A general method for the analysis of barbiturates, using column parition chromatography, was extended to the assay of secobarbital, amobarbital, and pentobarbital. A solution of the barbiturate constitutes the immobile phase in the chromatographic system. It is eluted with ether-isooctane (1+9) and passed onto a K3PO4 column, which retains the barbiturate while extraneous materials are washed out. The barbiturate is removed from the column with etherisooctane (3+1), extracted from the eluate with NH3, and measured spectrophotometrically.
D000654:Amobarbital / Q000032:analysis; D001463:Barbiturates / Q000032:analysis*; D002214:Capsules; D002845:Chromatography; D004304:Dosage Forms; D006801:Humans; D008722:Methods; D010424:Pentobarbital / Q000032:analysis; D012631:Secobarbital / Q000032:analysis; D012997:Solvents; D013053:Spectrophotometry; D013607:Tablets
D001463:Barbiturates; D002214:Capsules; D004304:Dosage Forms; D012997:Solvents; D013607:Tablets; D012631:Secobarbital; D000654:Amobarbital; D010424:Pentobarbital