Gel chromatography of steroid oestrogens on sephadex LH-20.
The behavior of 23 steroid oestrogens Sephadex LH-20 using six solvent systems was investigated. The fractions eluted by gel column chromatography were analysed by thin-layer chromatography and spectrometry. The method was used for the radiochemical purity of labelled compounds by isotopic dilution.
D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004958:Estradiol / Q000031:analogs & derivatives / Q000032:analysis; D004967:Estrogens / Q000032:analysis*; D004970:Estrone / Q000031:analogs & derivatives / Q000032:analysis; D007202:Indicators and Reagents; D013053:Spectrophotometry
D004967:Estrogens; D007202:Indicators and Reagents; D004970:Estrone; D004958:Estradiol