Renin secretion from rat renal cortical cell suspensions.
Renin secretion of rat renal cortical cell suspensions was studied as a functionof variations in the composition of the suspension media.  Renin secretion was determined by incubating samples of the suspension media with rat renin substrate and measuringthe angiotensin I generated by radioimmunoassay.  Increasing the medium sodium concentration (50-144 meq/liter) linearly decreased the rate of renin secretion.  This relationship was unaffected by adding ouabain (10 minus 3 M)  to the suspensions.  The addition of furosemide (from 10 minus 5 to 10 minus 3 M) stimulated renin secretion.  Considered with previous observations, these results suggest that renin secretionis controlled by some function of sodium on the lumenal boder of the macula densa cells.
D000804:Angiotensin II / Q000032:analysis; D000818:Animals; D003864:Depression, Chemical; D005665:Furosemide / Q000494:pharmacology; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D012083:Renin / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology
D000804:Angiotensin II; D010042:Ouabain; D005665:Furosemide; D012964:Sodium; D012083:Renin