The synthesis of prostaglandin F by human endometrium in organ culture.
Slices of human endometrium obtained from hysterectomy specimens were cultured for 48 hours in an organ culture medium supplemented with ethanol (control, vehicle), 17-beta-estradiol (.5 mug/ml), or progesterone (.5 mug/ml). Uncultured endometrial tissue, cultured tissues, and the media were assayed for prostaglandin F (PGF) by a radioimmunoassay technique. Hematoxylin and eosin and periodic acid-Schiff stain histologic controls were done on all tissues. The concentrations of PGF in picograms/milligram, corrected for percent recovery, in the differently treated tissues were: preculture 298; culture control 2210; estrogen-treated 2680; progesterone-treated 1260. All differences except those between estrogen and control (p greater than .10) and progesterone and control (p less than .10) are significant at the p = .02 level or better. Progesterone appears to inhibit PGF synthesis which occurs during in vitro culture of human endometrium; estrogen tended to increase PGF synthesis in this system.
D000328:Adult; D003470:Culture Media; D003864:Depression, Chemical; D004717:Endometrium / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D009924:Organ Culture Techniques; D011374:Progesterone / Q000494:pharmacology; D011460:Prostaglandins F / Q000032:analysis / Q000096:biosynthesis*; D013268:Stimulation, Chemical
D003470:Culture Media; D011460:Prostaglandins F; D011374:Progesterone; D004958:Estradiol