Activation of carcinogenic arylhydroxamic acids by human tissues.
Incubation of the carcinogenic arylhydroxamic acids N-hydroxy-N-2-fluorenylacetamide or N-hydroxy-N-4-biphenylacetamide and tRNA with 105,000 times g supernatants of homogenates of human small intestine, liver, or colon led to formation of arylamine-substituted nucleic acid adducts. These data indicated that enzymes of human tissues could activate arylhydroxamic acids by N leads to O acyl transfer. The unstable N-acetoxyarylamines formed by these enzymes reacted spontaneously with the tRNA to give covalently linked adducts with the nucleic acid.
D000217:Acyltransferases / Q000378:metabolism; D000611:Aminobiphenyl Compounds / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism*; D002474:Cell-Free System; D003600:Cytosol / Q000201:enzymology; D005449:Fluorenes / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000378:metabolism; D006881:Hydroxyacetylaminofluorene / Q000378:metabolism*; D007421:Intestine, Small / Q000201:enzymology / Q000648:ultrastructure; D012343:RNA, Transfer / Q000378:metabolism*
D000611:Aminobiphenyl Compounds; D002273:Carcinogens; D005449:Fluorenes; D006877:Hydroxamic Acids; C001365:N-hydroxy-4-acetylaminobiphenyl; D006881:Hydroxyacetylaminofluorene; D012343:RNA, Transfer; D000217:Acyltransferases