Membrane permeability changes during stimulation of isolated salivary glands of Calliphora by 5-hydroxytryptamine.
1. The membrane resistance of isolated salivary glands was found to decrease in response to 5-HT. The change in resistance was calcium-dependent. 2. The resistance change of the apical membrane was found to be much greater than the change in resistance of the basal membrane. 3. Potential responses under current-clamped conditions showed that one part of the biphasic response to 5-HT (attributed to an increase in chloride permeability) could be reversed and the other part (attributed to an increase in a potassium pump) could not. 4. These observations have been incorporated into a model which, on evaluation, predicts all of the observed potential changes during the action of 5-HT. It  suggests that the potential responses reflect changes in the internal chloride concentration produced by the calcium-dependent increases in chloride permeability.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002712:Chlorides / Q000378:metabolism; D003470:Culture Media; D004175:Diptera / Q000502:physiology*; D004553:Electric Conductivity / Q000187:drug effects; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D008954:Models, Biological; D012469:Salivary Glands / Q000187:drug effects* / Q000378:metabolism; D012701:Serotonin / Q000494:pharmacology*
D002712:Chlorides; D003470:Culture Media; D012701:Serotonin; D002118:Calcium