Evaluation of altered blood-brain barrier permeability to amino acids.
The blood-brain barrier (BBB) in one cerebral hemisphere of rabbit was damaged by the ipsilateral infusion of hypertonic (3 Molar) urea into the carotid artery. Using the indicator diffusion technique, the clearance of the glycine (by simple diffusion) and phenylalanine molecule (by facilitated diffusion) was enhanced in the urea infused cerebral hemisphere. The experimental methodology is a nondestructive and sensitive method for evaluating the integrity of the BBB.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D005998:Glycine / Q000378:metabolism; D006982:Hypertonic Solutions; D010539:Permeability; D010649:Phenylalanine / Q000378:metabolism; D011817:Rabbits; D014508:Urea / Q000494:pharmacology
D000596:Amino Acids; D006982:Hypertonic Solutions; D010649:Phenylalanine; D014508:Urea; D005998:Glycine