Origin and partial characterization of Fc receptor-bearing cells found within experimental carcinomas and sarcomas.
A variety of murine connective and epithelial tissue tumors, including the SAD/2 and FS9 fibrosarcomas, the TA3/Ha and CAD/2 mammary carcinomas and a primary methylcholanthrene-induced sarcoma, were found to contain a high proportion of cells with receptors for the Fc portion of immunoglobulin G ("Fc receptors"). Experiments were undertaken to assess whether these cells were neoplastic, or whether they represented the infiltration into the tumor of non-malignant host cells such as macrophages or lymphocytes. It was found that long-term established in vitro cell lines of the TA3/Ha SAD/2 and CAD/2 tumors were entirely negative for the Fc receptor, whereas injection of these cells led to the formation of tumors containing a high proportion of Fc receptor-bearing cells. Many of these cells were actively phagocytic as assessed by ingestion of iron filings or antibody-coated erythrocytes. Injection of Fc receptor-negative cultured tumor cells into F1 hybrids, in which host cells could be distinguished from the tumor cells by anti-H2 sera, revealed that many or all of the Fc receptor-bearing cells in the resultant tumor were of host origin. In contrast to its effect on normal spleen cells, anti-theta serum treatment also partially inhibited Fc rosettes, suggesting a T-lymphocyte origin for some of the Fc receptor-bearing cells. Since almost all cells with potential anti-tumor activity bear Fc receptors, it is suggested that an index of host cell infiltration of carcinomas and sarcomas can quickly and easily be ascertained by enumeration of Fc receptor-bearing cells.
D000818:Animals; D001666:Binding Sites, Antibody; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology; D002460:Cell Line; D003238:Connective Tissue / Q000276:immunology; D003239:Connective Tissue Cells; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007104:Immune Adherence Reaction; D007106:Immune Sera; D007141:Immunoglobulin Fc Fragments; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008325:Mammary Neoplasms, Experimental / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects; D011947:Receptors, Antigen, B-Cell; D012513:Sarcoma, Experimental / Q000276:immunology*; D012756:Sheep / Q000276:immunology; D013268:Stimulation, Chemical; D014357:Trypsin / Q000494:pharmacology
D007106:Immune Sera; D007141:Immunoglobulin Fc Fragments; D011947:Receptors, Antigen, B-Cell; D014357:Trypsin