Cell population kinetics in the rat jejunal crypt.
Cell kinetics in the jejunal crypt of the male Wistar rat were studied using autoradiographic techniques with tritiated thymidine and a stathmokinetic technique with vincristine. The migration rate measured by following the movement of the 50% peak on the labelling index distribution curve with time after injection of tritiated thymidine gave a value of 1-43 +/- 0-14 (SE) cell positions per hour, compared with a value from a cumulative birth rate of 1-78 cell positions per hour. Tht crypt column length was 32-9 +/- 0-2 cells and the column count was 22-3 +/- 0-2. This measurement gave a total crypt population of 734 cells, compared with an estimate of 650 +/- l from direct observation of squashed, microdissected crypts. In each crypt 22-5 +/- 0-5 mitoses were present, and the crypt cell production rate was 32 cells per crypt per hour; this latter value was confirmed using two independent techniques. The crypt growth fraction calculated from the durations of phases of the cell cycle and the labelling index was 0-62. A value of 0-61 was found from the labelling index distribution curve. As assessed from crypt squashes, there were 403 proliferating cells per crypt.
D000818:Animals; D001345:Autoradiography; D002452:Cell Count; D002455:Cell Division; D002465:Cell Movement; D007583:Jejunum / Q000166:cytology*; D007700:Kinetics; D008297:Male; D008938:Mitosis; D008940:Mitotic Index; D051381:Rats; D013936:Thymidine; D014316:Tritium; D014750:Vincristine
D014316:Tritium; D014750:Vincristine; D013936:Thymidine