An approach to the numerical quantitation of acute tissue response to biomaterials.
The hydrolytic enzyme activity associated with the tissue reaction to implanted polyvinyl chloride rods containing graded concentrations of an organotin stabilizer was quantitated by microphotometry of tissue sections. The procedure was more sensitive to the effects of the additive than the usual histologic evaluation. It is considered that this type of approach offers a sensitive and objective index to supplement the more subjective morphologic observations in judging the acute histotoxicity of implants.
D000135:Acid Phosphatase / Q000378:metabolism; D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006867:Hydrolases / Q000378:metabolism*; D008264:Macrophages / Q000201:enzymology; D009132:Muscles / Q000187:drug effects / Q000201:enzymology / Q000601:surgery; D009947:Organotin Compounds / Q000494:pharmacology; D010783:Photometry; D010795:Phthalic Acids / Q000494:pharmacology*; D011143:Polyvinyl Chloride / Q000494:pharmacology*; D011145:Polyvinyls / Q000494:pharmacology*; D051381:Rats; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D001672:Biocompatible Materials; D009947:Organotin Compounds; D010795:Phthalic Acids; D011145:Polyvinyls; D013438:Sulfhydryl Compounds; D011143:Polyvinyl Chloride; D006867:Hydrolases; D000135:Acid Phosphatase; D000626:Aminopeptidases