Gas-liquid chromatographic determination of strychnine in grain baits.
A simple and rapid gas-liquid chromatographic procedure, using a 6' times 1/4'' glass column packed with 5% SE-30 on Chromosorb W (DMCS) and a flame ionization detector, is described. Grain baits containing strychnine alkaloid are ground, mixed, and extracted by shaking with chloroform containing an internal standard, 1,3,5-triphenylbenzene. Without further cleanup, extract filtrates are injected directly into a gas chromatograph. Peak height ratios are used for quantitation of strychnine. The analysis of commercial samples shows that the method compares well with a commonly employed ultraviolet spectrophotometric method; good precision, with recoveries ranging from 89.9 to 91.7%, is obtained in the analysis of prepared samples. The method is sensitive to 2 mug strychnine.
D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000032:analysis*; D008832:Microchemistry; D012378:Rodenticides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013331:Strychnine / Q000032:analysis*
D012378:Rodenticides; D013331:Strychnine