In vivo incorporation of cytidine-monophosphate-ciliatine into rat liver lipids.
1. In vivo this investigation was carried out in order to compare the incorporation into rat lipids of free [1,2-minus 14C]-ciliatine and CMP-[1,2-minus 14C]-ciliatine which is the precursor in phosphonolipid biosynthesis. 2. The incorporation of the radioactivity from CMP-[1,2-minus 14C]-ciliatine took place more rapidly than that from free [1,2-minus 14C]-ciliatine in both liver and kidney. The amount of radioactivity from the CMP-[1,2-minus 14C]-ciliatine incorporated into total liver lipids was about 5 times higher than that incorporated into total liver lipids of rat two hrs after injecting free-[1,2-minus 14C]-ciliatine. 3. The amount of [1,2-minus 14C]-ciliatine incorporated into total liver lipids was 15 and 21 times higher than that incorporated into total kidney lipids of rat two and four hrs after injecting free [1,2-minus 14C]-ciliatine. 4. If the main pathway for the phosphonolipid biosynthesis is via CMP-ciliatine, the rate of phosphonolipid formation from CMP-ciliatine must therefore be higher than that from free-ciliatine. The results obtained here indicate therefore that the main pathway for phosphonolipid biosynthesis is a pathway involving CMP-ciliatine. 5. An unknow compound was detected in the water soluble fraction of the acid hydrolyzate of liver phosphonolipids. This material migrated with the N-trimethyl-derivative of ciliatine on the thin-layer chromatogram. The result shows that there is therefore a possibility of methylation of exogenous ciliatine to the phosphonate analogue of choline in the mammalian body.
D000615:Aminoethylphosphonic Acid / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003597:Cytosine Nucleotides / Q000378:metabolism*; D004983:Ethanolamines / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D008297:Male; D063065:Organophosphonates / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D010758:Phosphorus / Q000032:analysis; D051381:Rats
D002250:Carbon Radioisotopes; D003597:Cytosine Nucleotides; D004983:Ethanolamines; D008055:Lipids; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D010743:Phospholipids; D010758:Phosphorus; D000615:Aminoethylphosphonic Acid