Electrophoretic separation of serum proteins on cellulose acetate followed by electrophoresis in antibody - containing agarose gel.
A modification of the crossed immunoelectrophoresis of serum proteins is described in which the first step is carried out on cellulose acetate membranes and the second in antibody-containing agarose gel. The use of a special chamber allows reproducible conditions. The cellulose acetate membranes with the protein fractions separated in the first step can, if desired, be stored at minus 20 degrees C until further use. The technique of reversed intermediate gel has been worked out and employed for the identification of the Laurell peaks and their localization in the pherogram. Crossed immunoelectrophoresis with cellulose acetate and agarose is simple, provides reproducible results and requires no apparatus other than a special gel chamber.
D000906:Antibodies; D001798:Blood Proteins / Q000302:isolation & purification*; D003429:Cross Reactions; D004355:Drug Stability; D004586:Electrophoresis; D004588:Electrophoresis, Cellulose Acetate; D005615:Freezing; D006801:Humans; D007122:Immunoelectrophoresis; D008722:Methods; D008855:Microscopy, Electron, Scanning; D012685:Sepharose
D000906:Antibodies; D001798:Blood Proteins; D012685:Sepharose