Hematopoiesis in the embryonic mouse spleen. II. Alterations after phenylhydrazine administration to the mothers.
The effect of phenylhydrazine (PHZ) on the hematopoietic events in the embryonic spleen of C57Bl/6J mice was examined by light and electron microscopy. Following PHZ in injections to the mothers, the embryonic spleen revealed a marked increase in erythroid precursors, with a shift to mature cells. This phenomenon was part of a more generalized stimulation of erythorpoiesis, expressed by a shift to mature red cell precursors in the embryonic livers and an increase in the percentage of non-nucleated cells in the embryonic peripheral blood. Concomitantly stimulation of phagocytosis in the spleen of embryos in the early gestational days and increased vascularity were observed, and a later effect of granulocytopoietic stimulation. The effect on erythropoiesis in the embryonic spleen might be a sequence of erythropoietin stimulation, either in the mothers or the fetuses, due to anemia and hypoxia following PHZ injections.
D000740:Anemia / Q000139:chemically induced; D000818:Animals; D004900:Erythroblasts / Q000648:ultrastructure; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D006413:Hematopoietic System; D008099:Liver / Q000187:drug effects; D051379:Mice; D008854:Microscopy, Electron; D010587:Phagocytosis; D010659:Phenylhydrazines / Q000494:pharmacology*; D011247:Pregnancy; D013154:Spleen / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure
D010659:Phenylhydrazines; D004921:Erythropoietin