Production of anti-W24 xenoantisera in rabbits.
One out of four rabbits injected with partially purified soluble HL-A antigen from serum produced cytotoxic antibodies to human lymphocytes. These antibodies appear to be directed to the specificity W24 as determined by correlation studies with HL-A alloantisera and by absorption-inhibition experiments with human platelets and with serum soluble HL-A antigens. In the sera from the other three immunized rabbits, no antibodies to human lymphoid cells could be detected by the cytotoxic test, blocking of T cell rosette formation, stimulation of DNA synthesis and inhibition of mixed lymphocyte reaction.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007106:Immune Sera; D007114:Immunization; D008214:Lymphocytes / Q000276:immunology; D011817:Rabbits / Q000276:immunology*; D013045:Species Specificity
D000906:Antibodies; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007106:Immune Sera; D003165:Complement System Proteins