Modification of the template capacity of liver chromatin for form-B ribonucleic acid polymerase by food intake in rats under controlled feeding schedules.
Nuclei from liver of rats accustomed to eating during the first 8h of a daily 12h dark period demonstrate an increased capacity to synthesize RNA 6H after the beginning of the feeding period. 2. This increase is accompanied by a higher yield of extractable form-B DNA-dependent RNA polymerase activity. 3. The endogenous RNA polymerase activity associated with nuclear chromatin is also stimulated by food intake. Both purified and chromatin-associated form-B enzyme activities exhibit different ionic strength requirements after food intake. 4. The sensitivity of exogenous (added) form-B-enzyme to changes in ionic strength changes after feeding when chromatin is used as template. 5. Chromatin extracted from the liver of fed rats is a better template for form-B-enzyme than chromatin extracted from starved rats.
D000645:Ammonium Sulfate; D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D004247:DNA; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005502:Food; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D013698:Templates, Genetic; D013997:Time Factors
D002843:Chromatin; D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D000645:Ammonium Sulfate