Modulation by prostaglandins of contractions in guinea-pig ileum.
A high concentration of indomethacin (40mu-g/ml) substantially reduced contractions of guinea-pig isolated ileum in Krebs solution to nerve stimulation with electrical pulses or nicotine. Responses to acetylcholine and histamine were also inhibited, but to a smaller extent. Low concentrations of prostaglandin E-2 (2 or 4ng/ml) mainly restored all the excitatory responses. Using a modified bathing solution (lacking in phosphate and with some other changes) indomethacin 0.36mu-g/ml selectively inhibited nerve-mediated contractions. The results explain differences in various reports, and support the possibility that prostaglandins modulate the response to cholinergic nerve activity.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D003864:Depression, Chemical; D004558:Electric Stimulation; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects* / Q000502:physiology; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009539:Nicotinic Acids / Q000494:pharmacology; D011458:Prostaglandins E / Q000378:metabolism / Q000494:pharmacology*; D013268:Stimulation, Chemical
D009539:Nicotinic Acids; D011458:Prostaglandins E; D006632:Histamine; D000109:Acetylcholine; D007213:Indomethacin