[Isolation of oligonucleotides and aminoacylated nucleotides on a boron-arylated polymer].
2',3'-diol groups-containing oligonucleotides were shown to be adsorbed on N,N-diethyl-N-(p-methylphenylboryl) aminoethylsephadex A-25, forming the complex between boryl groups of the polymer and cys-diol part of oligonucleotides. This way the mixture of oligonucleotides pNn + (Np) n-1 Np can be fractionated. The procedure described is suitable also for the isolation of pure aminoacyl-nucleotides having free cys-diol groups in a carbohydrate part of the molecule.
D000215:Acylation; D000586:Amination; D001896:Boron Compounds; D002845:Chromatography / Q000379:methods*; D009711:Nucleotides / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000302:isolation & purification*; D011108:Polymers
D001896:Boron Compounds; D009711:Nucleotides; D009841:Oligonucleotides; D011108:Polymers
Vydelenie oligonukleotidov i aminoatsilirovannykh nukleotidov na borarilirovannom polimere