Lectin agglutinability of non-neoplastic and neoplastic human lymphoid cells in vitro.
Agglutination by two lectins, Concanavalin A (Con A) and Ricinus communis agglutinin (RCA), has been investigated in a human lymphoid cell system. The main conclusions of this study are: (1) no systematic correlation exists between the neoplastic state and sensitivity to Con A or RCA; (2) cells of neoplastic lines vary unsystematically in their surface properties as evaluated by Con A agglutination, with the possible exception that presence of Epstein-Barr virus (EBV) is associated with a high degree of agglutination and (3) cells of diploid lymphoblastoid lines and phytohemagglutinin (PHA)-stimulated lymphoctes agglutinate similarly and significantly better than unstimulated T- or B-lymphocytes. The relatively simple Con A agglutination assay can be used as an adjunct in classification of human lymphoid cell lines.
D000371:Agglutination; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology*; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology*; D007938:Leukemia / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology* / Q000473:pathology; D008223:Lymphoma / Q000276:immunology; D009101:Multiple Myeloma / Q000276:immunology; D009369:Neoplasms / Q000473:pathology*; D009439:Neuraminidase / Q000494:pharmacology; D037121:Plant Lectins; D010947:Plants, Toxic; D012278:Ricinus; D013268:Stimulation, Chemical; D014357:Trypsin / Q000494:pharmacology
D037102:Lectins; D037121:Plant Lectins; D003208:Concanavalin A; D009439:Neuraminidase; D006821:Hyaluronoglucosaminidase; D014357:Trypsin