Open-flask alkaline hydrolysis of amino acid phenylthiohydantoin derivatives.
It is suggested that the open-flask system for the acid hydrolysis of proteins described earlier can also be used to advantage for the alkaline hydrolysis of amino acid phenylthiohydantoin derivatives obtained in protein sequential analysis according to Edman. Results obtained under nitrogen and argon atmospheres are reported for the hydrolysis of 0.5 mumole of each phenylthiohydantoin derivative, which is the amount of protein that is usually employed in an automatic sequenator.
D000468:Alkalies; D000596:Amino Acids / Q000032:analysis*; D001128:Argon; D002845:Chromatography; D006827:Hydantoins / Q000032:analysis*; D006868:Hydrolysis; D009584:Nitrogen; D010669:Phenylthiohydantoin / Q000032:analysis*; D011492:Protein Hydrolysates / Q000032:analysis
D000468:Alkalies; D000596:Amino Acids; D006827:Hydantoins; D011492:Protein Hydrolysates; D010669:Phenylthiohydantoin; D001128:Argon; D009584:Nitrogen