Defective thyroglobulin synthesis in an experimental rat thyroid tumor: iodination and thyroid hormone synthesis in isolated tumor thyroglobulin.
Purified rat tumor thyroglobulin from the experimental rat thyroid tumor, line 1-1C2, was studied for its thyroid hormone content after in vivo and in vitro iodination and compared with normal and desialylated normal rat thyroglobulin. Tumor thyroglobulin had a very low sialic acid and iodine content; after in vivo iodination it contained only small amounts of triiodothyronine (T3) and no detectable thyroxine (T4). After in vitro iodination with 125I it showed a distribution of T3 and T4 very similar to that of normal and desialylated normal thyroglobulin iodinated in vitro. In vitro iodination dissociated tumor and desialylated normal thyroglobulin to a greater extent than normal thyroglobulin. Tumor tissue, on the other hand, showed considerable iodinating activity in the 105,000 X g pellet when studied with exogenous acceptors. These results are compatible with a role for sialic acid in the maturation and migration of thyroglobulin to the iodination site, rpovided that the intracellular distribution of the iodinating enzymes are normal.
D000818:Animals; D007455:Iodine / Q000378:metabolism*; D007457:Iodine Radioisotopes; D009374:Neoplasms, Experimental / Q000378:metabolism; D051381:Rats; D012794:Sialic Acids / Q000032:analysis; D013954:Thyroglobulin / Q000096:biosynthesis*; D013961:Thyroid Gland / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism*; D013974:Thyroxine / Q000096:biosynthesis*; D013997:Time Factors; D014284:Triiodothyronine / Q000096:biosynthesis*
D007457:Iodine Radioisotopes; D012794:Sialic Acids; D014284:Triiodothyronine; D013954:Thyroglobulin; D007455:Iodine; D013974:Thyroxine