An improved method for plasma fibrinogen based on thrombin time measurement.
Methods for plasma fibrinogen based on thrombin time are technically simple, rapid, and sensitive, although "false low" results may occur due to heparin interference. The present modification incorporates Polybrene into buffer to eliminate this heparin interference. The proposed method shown excellent agreement with a reference procedure based on clottable protein, and excellent day-to-day precision (C.V.3.5%). The present method is easily adaptable to semi-automated measurements.
D001322:Autoanalysis; D001780:Blood Coagulation Tests; D002021:Buffers; D005069:Evaluation Studies as Topic; D005340:Fibrinogen / Q000032:analysis*; D006493:Heparin; D006801:Humans; D008071:Lipoprotein Lipase / Q000097:blood; D008722:Methods; D013917:Thrombin / Q000378:metabolism*; D013997:Time Factors
D002021:Buffers; D005340:Fibrinogen; D006493:Heparin; D008071:Lipoprotein Lipase; D013917:Thrombin