A growth medium for the production of a bacterin for immunization against infectious coryza.
A serum-free modified Casman broth medium was developed to grow Haemophilus gallinarum to titers of 10-8 organisms per ml. Formalin-inactivated bacterins prepared form the broth culture were used to immunize chickens. A single dose of bacterin containing adjuvant protected 6 of 7 chickens against a homologous challenge 4 weeks postvaccination. A second dose given 3 weeks after the first offered no better protection than a single dose.
D000818:Animals; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines / Q000008:administration & dosage; D002645:Chickens; D003470:Culture Media; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006190:Haemophilus / Q000254:growth & development* / Q000276:immunology; D006192:Haemophilus Infections / Q000517:prevention & control / Q000662:veterinary*; D007114:Immunization / Q000662:veterinary*; D007279:Injections, Subcutaneous; D011201:Poultry Diseases / Q000517:prevention & control*
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D003470:Culture Media; D005620:Freund's Adjuvant