Simple isolated perfused artery preparation: vasoconstrictor evaluation.
Small mesenteric arteries free of all extraarterial tissues were obtained from anesthetized dogs and perfused in vitro with Krebs solution. Vasoconstrictor responses of these arteries to intraarterial levarterenol and epinephrine were dose related and equivalent to those of arteries surrounded by fat and other tissues. Responsiveness was stable for at least 60 min. This simple preparation is useful for the study of vasoconstrictor phenomena uncomplicated arterial tissue.
D000818:Animals; D002404:Catheterization; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D007269:Injections, Intra-Arterial; D007421:Intestine, Small / Q000098:blood supply; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects*; D009638:Norepinephrine / Q000494:pharmacology; D010477:Perfusion / Q000379:methods; D012039:Regional Blood Flow / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D014662:Vasoconstrictor Agents; D009638:Norepinephrine; D004837:Epinephrine