Quantitative determination of cortisol in human plasma by high-pressure liquid chromatography.
A method is described for the determination of cortisol in human plasma by high-pressure liquid chromatography. The simplified extraction procedure makes the method applicable to routine clinical assays. Partition chromatography is carried out on a Zorbax-Sil column with the eluent system dichloromethane-ethanol-water. A 78% recovery was obtained for cortisol. The detection limit is 1 mug per 100 ml in 1 ml of plasma. Cortisol values were determined in samples from a random selection of patients.
D000293:Adolescent; D000328:Adult; D002648:Child; D002845:Chromatography / Q000379:methods*; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D008297:Male; D008722:Methods; D011239:Prednisolone / Q000097:blood; D011312:Pressure; D011863:Radioimmunoassay; D012997:Solvents
D012997:Solvents; D011239:Prednisolone; D006854:Hydrocortisone