GLC analysis of hydrochlorothiazide in blood and plasma.
GLC with electron-capture detection was successfully applied to the analysis of hydrochlorothiazide in human blood and plasma with a sensitivity (0.05 mug/ml) suitable for use with persons on therapeutic dosage levels. On-column methylation with trimethylanilinium hydroxide in methanol was employed to convert hydrochlorothiazide and its bromo analog, which served as the internal standard, to their tetramethyl derivatives.
D002849:Chromatography, Gas; D006801:Humans; D006852:Hydrochlorothiazide / Q000031:analogs & derivatives / Q000097:blood*; D008722:Methods
D006852:Hydrochlorothiazide