Synthesis of kallikreins by rat kidney slices.
1 Four radioactive akllikreins were isolated from rat kidney slices incubated with (3H)-L-leucine. 2 The kallikreins were purified by procedures previously used for the isolation of rat urinary kallikreins (Nustad & Pierce, 1974), and by affinity chromatography on a column of insolubilized anti-rat urinary kallikrein. 3 The kidney kallikreins resembled the urinary kallikreins in their relative amounts, isoelectric points and electrophoretic mobilities on polyacrylamide disc gels. 4 The data indicate that the kidney synthesizes four kallikreins which are released into urine. The kallikreins are not changed by passage through the lower urinary tract.
D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D007610:Kallikreins / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D007668:Kidney / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D051381:Rats; D012756:Sheep / Q000276:immunology
D011506:Proteins; D007610:Kallikreins; D007930:Leucine