Identifications of radioactive steroid estrogen conjugates in blood plasma of laying hens after intramuscular injection of (4--14C)-estrone.
[4--14C] Estrone was injected intramuscularly into six laying hens. Fifty minutes later the hens were exsanguinated. The plasmas were examined for conjugates of radioactive phenolic steroids by recovery on columns of Amberlite XAD-2 or by extraction with tetrahydrofuran followed by chromatography on a column of DEAE-Sephadex A-25 in a gradient of NaCl. The biggest Sephadex chromatographic fraction (50,4% of total) contained about 42% of its radioactivity as estradiol-17alpha-3-sulfate and 18% as estradiol-17beta-3-sulfate and the remaining 40% was identified tentatively as estradiol-17alpha-17-sulfate plus a small proportion of estradiol-17beta-17-sulfate. The second biggest Sephadex chromatographic fraction (12.7% of total) was a mixture of conjugates not further identified. Minor fractions identified comprised estrone-beta-glucuronide (2.8%), estradiol-17alpha-3-beta-glucuronide (2.8%), estradiol-17beta-3-beta-glucuronide (2.3%) and estrone sulfate (6.0%). Evidence was obtained for the presence of small proportions of estradiol-17alpha disulfate and estradiol-17beta disulfate.
D000818:Animals; D002250:Carbon Radioisotopes; D002645:Chickens; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004958:Estradiol / Q000097:blood; D004967:Estrogens / Q000097:blood*; D004970:Estrone / Q000008:administration & dosage* / Q000097:blood; D005260:Female; D005965:Glucuronates / Q000097:blood; D007273:Injections, Intramuscular; D008722:Methods; D013464:Sulfuric Acids / Q000097:blood; D014316:Tritium
D002250:Carbon Radioisotopes; D004967:Estrogens; D005965:Glucuronates; D013464:Sulfuric Acids; D014316:Tritium; D004970:Estrone; D004958:Estradiol