Biosynthesis of serum albumin in rat liver. Isolation and probable structure of 'proalbumin' from rat liver.
1. Two methods are described for the preparation of 'proalbumin' in good yields from rat liver. 2. One of the methods does not depend on the use of specific antisera. 3. The product from both methods is identical as judged by electrophoresis on polyacrylamide gel, isoelectric focusing on pH gradients, ion-exchange chromatography and quantitative immunoelectrophoresis. The protein also appears to be radiochemically pure by these criteria. 4. The protein is free from serum albumin as judged by isoelectric focusing and co-chromatography on ion-exchange columns. It is judged to be free from other proteins by these same criteria and by specific precipitation with antibody. 5. It is converted into serum albumin by limited tryptic hydrolysis. The albumin so produced has the same N-terminal (glutamic acid) and C-terminal (alanine) amino acids as reported for rat serum albumin. 6. A hexapeptide is liberated from the N-terminal end of 'proalbumin' simultaneously. It contains three arginine, one phenylalanine, one valine and one glycine residues.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D007106:Immune Sera; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D008722:Methods; D008862:Microsomes, Liver / Q000032:analysis; D011228:Prealbumin / Q000302:isolation & purification*; D051381:Rats; D012709:Serum Albumin / Q000096:biosynthesis / Q000302:isolation & purification*; D012756:Sheep / Q000276:immunology; D014357:Trypsin
D000596:Amino Acids; D007106:Immune Sera; D011228:Prealbumin; D012709:Serum Albumin; D014357:Trypsin