The use of cellulose xanthate for the immobilisation of biological molecules.
The mercapto groups of cellulose xanthate can reversibly form disulphide bridges with L-cysteine. This property has been utilised for the immobilisation of a protein and an enzyme. These macromolecules, as polythiol derivatives, formed disulphide linkages with the matrix without serious disturbance of their active sites, became firmly bound to the xanthate, and were not eluted by normal washing conditions. Cellulose xanthate is a cheap, easily prepared matrix which permits a simple coupling reaction. The immobilisation process is selectively reversible.
D001665:Binding Sites; D002482:Cellulose / Q000031:analogs & derivatives*; D003545:Cysteine; D004220:Disulfides; D004798:Enzymes; D008722:Methods; D011485:Protein Binding; D011506:Proteins; D012995:Solubility; D014970:Xanthines
D004220:Disulfides; D004798:Enzymes; D011506:Proteins; D014970:Xanthines; C528876:cellulose xanthate; D002482:Cellulose; D003545:Cysteine