Quantitative determination of alkaloids in belladonna tincture USP.
A simplified method for the quantitative analysis of hyoscyamine hydrobromide or atropine in Belladonna Tincture USP is described. The procedure is based on the formation of an alkaloid-dye complex which can be extracted with an organic solvent and measured spectrophotometrically. The results obtained by this method compare favorably with those obtained by the USP method.
D001532:Atropa belladonna / Q000032:analysis*; D001285:Atropine / Q000032:analysis; D001533:Belladonna Alkaloids / Q000032:analysis*; D001979:Bromthymol Blue; D008722:Methods; D010946:Plants, Medicinal; D010947:Plants, Toxic; D013056:Spectrophotometry, Ultraviolet
D001533:Belladonna Alkaloids; D001285:Atropine; D001979:Bromthymol Blue