[Toxigenesis of Clostridium onvyi type A. 1. Communication: production of alpha-toxin in vitro (author's transl)].
The production and excretion of alpha-toxin in vitro was studied with two pathogenetically different strains of Cl. novyi type A. During the exponential phase of growth nearly all alpha-toxin produced by strain 955 was concentrated within the cell, while only small amounts of toxin could be detected in the culture medium. On the contrary, alpha-toxin produced by strain Fö during the exponential phase of growth was found in equal parts (240 DLM/2 times 10(8) cells) within and outside the cell. During the stationary phase of growth 50 to 75% of the intracellular alpha-toxin was released into the culture fluid. The different rates of excretion of alpha-toxin may be the reason for the differing phenomena in gas gangrene of guinea pigs infected with Cl. novyi.
D000818:Animals; D002498:Centrifugation; D003013:Clostridium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D003015:Clostridium Infections; D003470:Culture Media; D004487:Edema; D004731:Endotoxins / Q000032:analysis / Q000096:biosynthesis* / Q000633:toxicity; D005110:Extracellular Space / Q000032:analysis; D006168:Guinea Pigs; D007424:Intracellular Fluid / Q000032:analysis; D010400:Penicillin G / Q000494:pharmacology; D013010:Sonication
D003470:Culture Media; D004731:Endotoxins; D010400:Penicillin G
Zur Toxigenese von Clostridium novyi Typ A. 1. Mitteilung: alpha-Toxin-Bildung in vitro