Assay and relationship of HT-2 toxin and T-2 toxin formation in liquid culture.
Both T-2 toxin and HT-2 toxin can be conveniently quantitated in crude extracts by using a combination of thin-layer chromatography and fluorodensitometry. This technique was used to follow the production of these toxins by liquid cultures of Fusarium poae (NRRL 3287). T-2 toxin was produced prior to HT-2 toxin and hexadeuterio-T-2 toxin was converted by the culture to trideuterio-HT-2 toxin.
D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D003470:Culture Media; D003720:Densitometry; D005670:Fusarium / Q000032:analysis / Q000378:metabolism*; D012717:Sesquiterpenes / Q000096:biosynthesis*; D014255:Trichothecenes / Q000032:analysis / Q000096:biosynthesis*
D003470:Culture Media; D012717:Sesquiterpenes; D014255:Trichothecenes