Separation and determination of carnitine and its esters in human serum.
A colorimetric method for the determination of carnitine and its derivatives is described. Free carnitine, acetylcarnitine and palmitoylcarnitine were extracted with chloroform-methanol. After evaporation, the residue was dissolved in n-butanol and water; palmitoylcarnitine in the organic phase, and free carnitine and acetylcarnitine by passing the aqueous phase through a column of Amberlite CG-120 (H+). Separated carnitine derivatives were hydrolyzed to free carnitine which was followed by the Amberlite column chromatography. After eluting with 2N NH4OH solution, the samples were esterified and determined colorimetrically.
D002331:Carnitine / Q000097:blood*; D003124:Colorimetry / Q000379:methods; D004952:Esters / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods
D004952:Esters; D002331:Carnitine