Implication of ligand modified spectra of cytochrome P-450 associated with pregnenolone synthesis in mitochondria from corpus luteum.
The implications of ligand modified spectra of cytochrome P-450 in mitochondria from corpus luteum are considered. Mitochondria from bovine corpus luteum contain a single cytochrome P-450 which oxidizes cholesterol to pregnenolone and isocaproaldehyde. These mitochondria and the cytochrome P-450 purified from these mitochondria yield Type I spectral changes with substrates, reverse Type I spectral changes with certain steroid substrates, steroid products and unrelated steroid ligands. Nitrogenous ligands yield Type II spectral changes. Mitochondrial and purified cytochrome P-450 preparations are equivalent in this study. The inhibitory effects on the cholesterol monooxygenase are directly related to the spectral changes induced by Type II ligands. Lastly, it is suggested that a similar relationship exists with reverse Type I ligands.
D000616:Aminoglutethimide / Q000494:pharmacology; D000818:Animals; D001593:Benzyl Compounds / Q000494:pharmacology; D002248:Carbon Monoxide / Q000494:pharmacology; D002417:Cattle; D002784:Cholesterol / Q000378:metabolism; D002793:Cholic Acids / Q000494:pharmacology; D003338:Corpus Luteum / Q000378:metabolism*; D003489:Cyanoketone / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005260:Female; D006888:Hydroxycholesterols / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology; D008024:Ligands; D008928:Mitochondria / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D011284:Pregnenolone / Q000096:biosynthesis* / Q000494:pharmacology; D013057:Spectrum Analysis
D001593:Benzyl Compounds; D002793:Cholic Acids; D006888:Hydroxycholesterols; D007093:Imidazoles; D008024:Ligands; D000616:Aminoglutethimide; D003489:Cyanoketone; D011284:Pregnenolone; D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D010088:Oxidoreductases