Association of endonuclease activity with serotypes belonging to the three subgroups of human adenoviruses.
Endonuclease activity has been detected in association with highly purified virions, pentons, and/or dodecons of adenovirus types 2, 3, 5, 9, 12, 15, and 16. Only single-strand scissions in substrate DNA were detected. The nuclease activity was detected by a highly sensitive ethidium bromide fluorimetric assay procedure.
D000256:Adenoviridae / Q000201:enzymology*; D001435:Bacteriophages; D002277:Carcinoma; D002460:Cell Line; D004267:DNA Viruses; D004270:DNA, Circular / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*; D004996:Ethidium; D005470:Fluorometry; D006367:HeLa Cells; D006801:Humans; D009062:Mouth Neoplasms; D011549:Pseudomonas; D012703:Serotyping; D014764:Viral Proteins / Q000378:metabolism
D004270:DNA, Circular; D004279:DNA, Viral; D014764:Viral Proteins; D003851:Deoxyribonucleases; D004720:Endonucleases; D004996:Ethidium