Quantitative method for estimating fecal amino acids.
We describe a method for quantitatively estimating 24 ninhydrin-reacting substances, including the commoner amino acids, in fecal dialysate prepared from ingested dialysis bags retrieved from fresh stool. It is accurate to 2 mumol of alpha-amino nitrogen per liter of fecal dialysate, and for most substances recovery of added standards is 100%. It involves dilution, ultrafiltration, and automated ion-exchange column chromatography of the dialysate. Some normal values are provided.
D000328:Adult; D000596:Amino Acids / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D003956:Dialysis; D005243:Feces / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D014462:Ultrafiltration
D000596:Amino Acids