Pre-ovulatory elevation of rat ovarian prostaglandins F, and its blockade by indomethacin.
Prostaglandins of the F series have been measured, by radioimmunoassay, in ovaries of prepubertal rats in which first estrus was synchronized by injection of pregnant mare serum gonadotropin. Marked elevations, up to 20-fold, were observed approximately in parallel with the endogenous luteinizing hormone surge which occurs on the evening of proestrus, and within 4 h after injection of exogenous luteinizing hormone on the morning of proestrus. Injection of indomethacin, 0.8 mg per rat, at 14:45 on proestrus, prevented the increase in ovarian prostaglandins F without significantly reducing the proestrous elevation of serum luteinizing hormone. These observations support elevation of serum luteinizing hormone. These observations support a physiologic role of prostaglandins F in rat ovarian responses to luteinizing hormone.
D000818:Animals; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000097:blood / Q000494:pharmacology; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D010060:Ovulation / Q000187:drug effects; D011460:Prostaglandins F / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011863:Radioimmunoassay; D051381:Rats; D013268:Stimulation, Chemical
D006064:Gonadotropins, Equine; D011460:Prostaglandins F; D007986:Luteinizing Hormone; D007213:Indomethacin