Demonstration by immunoelectro-osmophoresis of precipitating antibodies to a purified rubella virus antigen.
The nonionic detergent Triton X-100 was used to extract antigens of rubella virus from infected tissue culture cells. Three virus-specific antigens were demonstrated by crossed immunoelectrophoresis by using a pool of human gamma globulin as antiserum. The most dominant of these antigens were purified by ion-exchange chromatography on diethylaminoethyl-cellulose. This antigen was of glucoprotein nature and had slow electrophoretic motility and low binding capacity to diethylaminoethyl-cellulose. Thus, it seems likely that the antigen is identical with the precipitating antigen of rubella virus designated b-antigen or tro-osmophoresis with precipiting antibody in sera obtained from patients recovering from acute postnatal rubella. The precipitin reaction that could be correlated to the hemaglutination-inhibition titers of the same sera appeared 12 days after onset of the disease and remained positive for several years.
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000302:isolation & purification; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D003315:Cornea; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D005944:Glucosamine / Q000378:metabolism; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007122:Immunoelectrophoresis; D010047:Ovalbumin; D011233:Precipitin Tests; D011817:Rabbits; D012412:Rubella virus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D002250:Carbon Radioisotopes; D010047:Ovalbumin; D005944:Glucosamine