Fluorocarbon aerosol propellants V: binding interaction with human albumin.
Binding of trichloromonofluoromethane, dichlorodifluoromethane, and dichlorotetrafluoroethane was studied in aqueous 5% human albumin solution, using the partition coefficient method in sealed serum bottles. The partition coefficient and the fraction of fluorocarbons bound were highly dependent on fluorocarbon concentrations. The average binding sites per molecule of albumin were 2.17, 0.30, and 0.42 and the binding association constants were 1.11 X10-3, 1.73 X10-3, and 5.06X10-3 M-minus 1, respectively. At the lowest concentration studied, 62.3, 25.5, and 65.6% were found bound to albumin, respectively. This appears to represent the first extensive study on any gas-albumin interaction.
D000335:Aerosol Propellants / Q000494:pharmacology*; D000336:Aerosols / Q000494:pharmacology*; D001665:Binding Sites; D001667:Binding, Competitive; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005465:Fluorocarbon Polymers / Q000494:pharmacology*; D005466:Fluorocarbons / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D012709:Serum Albumin / Q000378:metabolism*
D000335:Aerosol Propellants; D000336:Aerosols; D005465:Fluorocarbon Polymers; D005466:Fluorocarbons; D012709:Serum Albumin