Isolation, ultrastructure and chemical composition of the outermost layer ("exo-layer") of the Epidermophyton floccosum cell wall.
The outer-most layer ("exo-layer") of the wall was isolated from cell walls of Epidermophyton floccosum. The pure cell walls, obtained by disruption in a Ribi cell fractionator, sonication and centrifugation, were digested with snail enzyme for 12 h. Thereafter, the exo-layer preparation was obtained as the fraction resistant to the snail enzyme. Electron microscopy showed that the exo-layer is a thin, stranded network structure 10-20 nm thick. Chemical analysis of the exo-layer showed that the main components are protein (63 percent), mannose (10 percent) and glucosamine (17 percent). Sodium dodecyl sulfate polyacrylamide gel electrophoresis has revealed that the main band is a glycoprotein containing mannose.
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002458:Cell Fractionation / Q000379:methods; D002473:Cell Wall / Q000032:analysis / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D004821:Epidermophyton / Q000032:analysis / Q000648:ultrastructure*; D005656:Fungal Proteins / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008854:Microscopy, Electron; D008970:Molecular Weight
D000596:Amino Acids; D002241:Carbohydrates; D005656:Fungal Proteins; D006023:Glycoproteins; D008055:Lipids