Oxalate accumulation from citrate by Aspergillus niger. II. Involvement of the tricarboxylic acid cyclase.
Carbon-14 was incorporated into oxalate and CO2 from either citrate-1,5-14C, succinate-1,4-14C, or fumarate-1,4-14C by cultures of Aspergillus niger pregrown on a medium which contained glucose as the sole carbon source and which did not allow citrate accumulation. In cell-free extracts of mycelium forming oxalate and CO2 from added citrate the following enzymes of the tricarboxylic acid (TCA) cycle were identified: citrate synthase CE 4.1.3.7), aconitate hydratase (EC4.2.1.3), NAD and NADP-dependent isocitrate dehydrogenase (EC 1.1.1.41, 1.1.1.42), (alpha-oxoglutarate dehydrogenase (EC 1.2.4.2), succinate dehydrogenase (EC 1.3.99.1), fumarate hydratase (EC 4.2.1.2), and malate dehydrogenase (EC 1.1.1.37). The in vitro activity of aconitate hydratase and of NADP-dependent isocitrate dehydrogenase was shown to be almost identical to the rate of in vivo degradation of citrate or to exceed this rate. The degradation of citrate to oxalate was inhibited completely by 9 mM fluoroacetate. It is concluded that the TCA cycle is involved in the formation of oxalate from citrate.
D000154:Aconitate Hydratase / Q000378:metabolism; D001230:Aspergillus / Q000201:enzymology*; D001234:Aspergillus niger / Q000201:enzymology*; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002950:Citrate (si)-Synthase / Q000378:metabolism; D002951:Citrates / Q000378:metabolism*; D002952:Citric Acid Cycle; D004798:Enzymes / Q000378:metabolism*; D005463:Fluoroacetates / Q000494:pharmacology; D005649:Fumarate Hydratase / Q000378:metabolism; D005650:Fumarates / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007658:Ketone Oxidoreductases / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D010070:Oxalates / Q000096:biosynthesis*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013386:Succinates / Q000378:metabolism
D002250:Carbon Radioisotopes; D002951:Citrates; D004798:Enzymes; D005463:Fluoroacetates; D005650:Fumarates; D010070:Oxalates; D013386:Succinates; D002245:Carbon Dioxide; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D007658:Ketone Oxidoreductases; D013385:Succinate Dehydrogenase; D002950:Citrate (si)-Synthase; D005649:Fumarate Hydratase; D000154:Aconitate Hydratase