A short-chain acyl-CoA: 1-alkyl-2-acyl-sn-glycerol acyltrasnferase from a microsomal fraction of the rabbit Harderian gland.
The 3-position of the alkyldiacylglycerols from the pink portion of the rabbit harderian gland is occupied exclusively by isovaleric acid. We describe a microsomal 1-alkyl-2-acyl-sn-glycerol acyltransferase from this gland which specifically incorporates short-chain acyl-CoA's into the 3-position of alkylacyl-glycerols. The enzyme is most active in the presence of CoA esters with chain lengths similar to isovaleric acid and is inactive in the presence of acetyl CoA and long-chain acyl-CoA's. No evidence was found for an enzyme that would transfer long-chain acyl-CoA's to the same substrate. The specificity of this acyltransferase can account for the exclusion of long-chain acyl moieties from the 3-position of the alkyldiacylglycerols in the harderian gland of rabbits.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002264:Carboxylic Acids; D002855:Chromatography, Thin Layer; D003065:Coenzyme A; D005989:Glycerides; D006243:Harderian Gland / Q000201:enzymology*; D007765:Lacrimal Apparatus / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology*; D011817:Rabbits; D013329:Structure-Activity Relationship; D014631:Valerates
D002264:Carboxylic Acids; D005989:Glycerides; D014631:Valerates; D000217:Acyltransferases; D003065:Coenzyme A