Selective effects of enucleation and transfer of heterologous nuclei on cytoplasmic organelles in Amoeba proteus.
The ultrastructural changes in the cytoplasm of lethal hybrids obtained by nuclear transplantation between different strains of Amoeba proteus were compared with those of enucleated amebae.  It was found that, whereas the Golgi complex and glycocalyx degenerated first in enucleated cells, mitochondria and endosymbiotes became abnormal first in the hybrids.  The selective effects are attributed to the presence of nucleic acids in the mitochondria and endosymbiotes and hence to the different interactions they would have with the nuclear genome.
D000656:Amoeba / Q000648:ultrastructure*; D002455:Cell Division; D003593:Cytoplasm; D006824:Hybridization, Genetic; D008854:Microscopy, Electron; D053652:Nuclear Transfer Techniques; D009696:Nucleic Acids / Q000032:analysis; D009940:Organoids; D014184:Transplantation, Homologous
D009696:Nucleic Acids