The urinary excretion of nucleosides of ribonucleic acid by patients with advanced cancer.
By means of a sensitive and specific method utilizing gas-liquid chromatography, the excretion levels for three nucleosides, degradation minor base products of ribonucleic acid, primarily transfer ribonucleic acid, were determined in 24-hour urine specimens from over 200 patients with solid tumor malignancies. These nucleosides were N2,N2-dimethylguanosine, l-methylinosine, and pseudouridine. When compared to normal control values, elevated levels of these compounds were found for patients in each of several tumor types studied. Increases in pseudouridine excretion suggest increased tumor transfer ribonucleic acid turnover; in addition, for the methylated nucleosides, higher than normal values may reflect enhanced transfer ribonucleic acid methylase activity of the neoplastic cells.
D002849:Chromatography, Gas / Q000379:methods; D006151:Guanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D007288:Inosine / Q000031:analogs & derivatives* / Q000652:urine; D009369:Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000652:urine*; D011560:Pseudouridine / Q000652:urine*; D012313:RNA / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives*; D012359:tRNA Methyltransferases / Q000378:metabolism
D006151:Guanosine; D011560:Pseudouridine; C008940:N(2),N(2)-dimethylguanosine; D007288:Inosine; D012313:RNA; D012343:RNA, Transfer; D012359:tRNA Methyltransferases; D014529:Uridine