[Effect of acid extraction of basic proteins on the binding of acridine orange by cell chromatin].
The binding of acridine orange to chromatin of fixed human lymphocytes is increased after stepwise removal of histones by hydrochloric acid. No changes in the dye-binding features of the cells were found after f1 histone extraction (0.01 N HC1). Additional extraction of f2 and f3 by 0.05 N HC1 (partial extraction) and by 0.25 N HC1 (more complete extraction) resulted in increased binding of acridine orange (1.9 and 2.5 of that of the control, respectively).9222
D000166:Acridines; D001798:Blood Proteins; D002843:Chromatin / Q000032:analysis*; D006657:Histones / Q000302:isolation & purification*; D006801:Humans; D006851:Hydrochloric Acid; D066298:In Vitro Techniques; D008214:Lymphocytes; D011485:Protein Binding
D000166:Acridines; D001798:Blood Proteins; D002843:Chromatin; D006657:Histones; D006851:Hydrochloric Acid
Vliianie kislotnoĭ ékstraktsii osnovnykh belkov na sviazyvanie khromatinom kletok akridinovnogo oranzhevogo