Specific anti-tumor responses by cultured immune spleen cells. I. In vitro culture method and initial characterization of factors which block immune cell-mediated cytotoxicity in vitro.
Spleen cells from BALB/c mice which either bore syngeneic sarcomas or were normal controls were cultured in vitro. The culture supernatants of spleen cells from tumor-bearing mice inhibited (blocked) specific cell-mediated cytotoxicity to the tumor borne by the spleen donor. They also mediated specific antiserum-dependent cytotoxicity with control lymphoid cells. The appearance of blocking activity in culture supernatants was prevented by lysis of theta-positive spleen cells with antiserum and complement. The blocking activity was removed by passing culture supernatants through an anti-mouse immunoglobulin affinity column and was recovered in a 3 M NaSCN eluate of the column.
D000818:Animals; D002460:Cell Line; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D007106:Immune Sera; D007136:Immunoglobulins; D051379:Mice; D012513:Sarcoma, Experimental / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*
D007106:Immune Sera; D007136:Immunoglobulins; D003165:Complement System Proteins