Thin-layer chromatographic method for determining carbamazepine and two of its metabolites in serum.
A sensitive and highly specific thin-layer chromatographic method for determining simulataneously serum levels of carbamazepine and two of its major metabolites, carbamazepine-10 11-epoxide and 10, 11-dihydroxycarbamazepine, is presented. Serum (1 mug) was spotted directly onto the thin-layer plate and, after irrigation, the separated spots were converted into fluorescing compounds by exposing the plates to hydrogen chloride gas for 5 min and then to strong ultraviolet radiation from a mercury lamp for 20 min. The fluorescence was measured quantitatively using a spectrofluorimeter equipped with a thin-layer chromatogram scanning attachment. Two microlitres of serum are sufficient for a duplicate determination.
D002220:Carbamazepine / Q000031:analogs & derivatives / Q000097:blood* / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D003720:Densitometry; D004852:Epoxy Compounds / Q000097:blood; D006801:Humans; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
D004852:Epoxy Compounds; D002220:Carbamazepine