[Nature of the effect "rapid oxygen consumption" during the introduction of tissue homogenate or a mitochondrial suspension into the polarographic cell].
After introducing rat brain homogenate or mitochondria into polarographic cell without adding the substrate a quick shift of recording pen from the point of initial concentration of oxygen towards zero is observed. According to Kotova and coworkers (Biochemia, 38, 287, 1973) this shift is accompanied by a decrease of oxygen concentration (less than oxygen rapid consumption greater than). We established that the size of this shift was not proportional to the quantity of homogenate. Boiled homogenate of the rat brain, liver DNA solution, starch gel and casein glue caused less than oxygen rapid consumption greater than. It was proposed to locate the point of the initial oxygen concentration on the polragoramme in the position of the recording pen after the introduction of homogenate into the polragoraphic cell.
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002364:Caseins; D004247:DNA; D005260:Female; D005782:Gels; D006358:Hot Temperature; D066298:In Vitro Techniques; D008099:Liver; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption; D011048:Polarography; D051381:Rats; D013213:Starch
D002364:Caseins; D005782:Gels; D013213:Starch; D004247:DNA
O prirode éffekta "bystrogo potrebleniia kisloroda" pri vnesenii gomogenata tkaneĭ ili suspenzii mitokhondriĭ v poliarograficheskuia iacheĭku