[Isolation and composition of a glycoprotein from lamb gastric mucosa].
A glycoprotein is isolated from lamb gastric mucosa. It is purified first by reduction with dithiothreitol and then by chromatography on CM-Sephadex followed by gelfiltration on Sephadex G-75. Its homogeneity is investigated by disc electrophoresis, immunoelectrophoresis, analytical ultracentrifugation and identification of the carboxy- and amino-terminal amino acids. This glycoprotein contains 56 p. cent carbohydrates, consisting of N-glycolyneuraminic acid, fucose, galactose, and hexosamines. Hexosamines and galactose are in equimolar ratio ; galactosamine and glucosamine in the ratio 1 :2. The amino acid composition shows that threonine, serine and proline account for half of the amino acid residues. The cysteine origin and function are discussed.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D004229:Dithiothreitol; D004589:Electrophoresis, Disc; D005643:Fucose / Q000032:analysis; D005753:Gastric Mucosa / Q000032:analysis*; D006023:Glycoproteins / Q000302:isolation & purification; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D007122:Immunoelectrophoresis; D012756:Sheep; D012794:Sialic Acids / Q000032:analysis; D014461:Ultracentrifugation
D000596:Amino Acids; D006023:Glycoproteins; D006595:Hexosamines; D006601:Hexoses; D012794:Sialic Acids; D005643:Fucose; D004229:Dithiothreitol
Isolement et composition d'une glycoprotéine de muqueuse gastrique d'agneau