Isolation of endonuclease from mammalian cells by DNA - cellulose chromatography.
By means of DNA-cellulose chromatography an enzyme with endonucleolytic activity has been isolated from nuclear acidic protein fraction of mammalian cells. The main active fraction, eluted at 0.7 M NaCl, effects the velocity sedimentation of UV-irradiated and alkylated DNA, resulting in a decrease of the molecular weight. The fraction is completely inactive using native as well as heat-denatured DNA.
D000818:Animals; D002482:Cellulose; D002846:Chromatography, Affinity; D006224:Cricetinae; D004247:DNA / Q000528:radiation effects; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000528:radiation effects; D004720:Endonucleases / Q000302:isolation & purification*; D010954:Plasmacytoma; D011830:Radiation Effects; D014466:Ultraviolet Rays
D004273:DNA, Neoplasm; D002482:Cellulose; D004247:DNA; D004720:Endonucleases