Growth hormone-release inhibiting hormone (GH-RIH) pathway of the rat hypothalamus revealed by the unlabeled antibody peroxidase-antiperoxidase method.
Utilizing the unlabeled antibody enzyme method, we investigated the distribution of hypothalamic elements immunoreactive with antibodies to growth hormone-release inhibiting hormone (GH-RIH). Immunostained elements, resembling neural processes, are distributed along a pathway corresponding to a portion of the tuberoinfundibular tract. However, GH-RIH fibers are caudal, dorsal and medial to LH-RH fibers detected by the same technique. Similar topographic arrangements are noted in coronal and sagittal sections. Comparable results were obtained with two different preparations of antisera to GH-RIH. No cell bodies specifically stained by anti-GH-RIH were detected. Our data agree with those of other investigators using immunohistochemical techniques.
D000818:Animals; D000936:Antigen-Antibody Complex; D005260:Female; D006651:Histocytochemistry; D007031:Hypothalamus / Q000276:immunology*; D008297:Male; D008473:Median Eminence / Q000378:metabolism; D009434:Neural Pathways; D009489:Neurosecretion; D010544:Peroxidases / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D051381:Rats; D013004:Somatostatin / Q000032:analysis* / Q000276:immunology
D000936:Antigen-Antibody Complex; D013004:Somatostatin; D010544:Peroxidases