Renin activity solid phase radioimmunoassay.
A solid-phase radioimmunoassay for measurement of generation angiotensin I (renin activity) in plasma is reported. The angiontensin I antisera were attached to diazotized arylamine and N-hydroxy succinimide controlled pore glass, and reacted with labelled and unlabelled angiotentin I for saturation analysis before and after one hour incubation. Chelating agents were used to inhibit the conversion of angiotensin I to angiotensin II in vitro. The assay is accurate, rapid and reproducible.
D000804:Angiotensin II / Q000276:immunology; D007457:Iodine Radioisotopes; D008722:Methods; D011863:Radioimmunoassay; D012083:Renin / Q000378:metabolism*; D013388:Succinimides / Q000276:immunology
D007457:Iodine Radioisotopes; D013388:Succinimides; D000804:Angiotensin II; D012083:Renin