The role of 5-S RNA in temperature-sensitive ribosomal RNA maturation.
The synthesis of 5-S RNA was found to be unchanged at both the permissive (33.5 degrees C) and non-permissive (38.5 degrees C) temperatures in a temperature-sensitive Baby Hamster Kidney cell line (BHK 21 ts 422 E) as measured relative to synthesis of 18-S rRNA. The 5-S RNA is shown to be associated with nucleolar ribonucleoprotein particles even though rRNA processing does not yield a functional 28-S rRNA at the non-permissive temperature. The amount of 5-S RNA found associated with the 80-S ribonucleoprotein particles was the same at the permissive and non-permissive temperatures, indicating that an aberrant 5-S RNA contribution to rRNA processing is not a primary cause for the temperature-sensitive lesion of rRNA maturation in this mutant cell line. The amount of 5-S RNA in nucleolar 80-S RNA particles indicated that the association of 5-S RNA with the rRNA precursor particle occurs before the cleavage step at which 32-S precursor RNA is produced.
D002458:Cell Fractionation; D002460:Cell Line; D002466:Cell Nucleolus / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D009154:Mutation; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology*; D012335:RNA, Ribosomal / Q000302:isolation & purification / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000032:analysis; D013696:Temperature
D012335:RNA, Ribosomal; D012261:Ribonucleoproteins; D012313:RNA