Effect of metabolic inhibitors on pyrogen production by rabbit leukocytes.
The metabolic inhibitors, actinomycin D, cycloheximide, puromycin dihydrochloride, puromycin aminonucleoside, and p-fluorophenylalanine did not inhibit the release of leukocytic pyrogen whether endotoxin was preincubated with cells for 20 min at 37 degrees C before addition of inhibitor or inhibitor was preincubated with cells for 1 hr before addition of endotoxin. On the other hand, cortison inhibited release of pyrogen under both experimental conditions. Poly(I): poly(C) was not effective in inducing rabbit leukocytes to produce an endogenous pyrogen.
D000818:Animals; D001833:Body Temperature Regulation; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004731:Endotoxins / Q000494:pharmacology*; D005260:Female; D006854:Hydrocortisone / Q000494:pharmacology; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D011070:Poly I-C / Q000494:pharmacology*; D011691:Puromycin / Q000494:pharmacology; D011692:Puromycin Aminonucleoside / Q000494:pharmacology; D011749:Pyrogens / Q000097:blood*; D011817:Rabbits; D012475:Salmonella; D010135:p-Fluorophenylalanine / Q000494:pharmacology
D004731:Endotoxins; D011749:Pyrogens; D003609:Dactinomycin; D011691:Puromycin; D011692:Puromycin Aminonucleoside; D010135:p-Fluorophenylalanine; D003513:Cycloheximide; D011070:Poly I-C; D006854:Hydrocortisone