Study of a yeast mutant with modified mitochondrial translocation of adenine nucleotides. I. Characterization of the op-1 mutant during mutagenesis with ethidium bromide.
The effect of ethidium bromide on the growth of a yeast mutant with an impaired mitochrondrial translocation system of adenine nucleotides (op-1 mutant) was investigated. It was found that the op-1 mutant stops growing both under growing and non-growing conditions after treatment with ethidium bromide and that the growth cannot be restored by adding low-molecular compounds to the growth medium. It was the aim of the experiments to clarify whether the cessation of growth of the op-1 mutant after induction of the rho- mutation can be simulated by inhibitors phenotypically changing the mitochondrial function. It appears likely that the op-1 mutant stops growing only after the rho- mutation has been induced, because the phenotypic simulation of the rho- mutation does not lead the cessation of growth of the op-1 mutant.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002455:Cell Division; D003470:Culture Media; D004996:Ethidium / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D009153:Mutagens; D009154:Mutation; D010101:Oxygen Consumption; D015003:Yeasts / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D000227:Adenine Nucleotides; D000900:Anti-Bacterial Agents; D003470:Culture Media; D009153:Mutagens; D004996:Ethidium