Alkaloids of tobacco and blood coagulation: effect of nicotine on thrombin and fibrinogen.
An alkaloid of tobacco, nicotine, affected the clot-formation property of the enzyme, thrombin, on the substrate, plasma or fibrinogen (thrombin time). Higher concentrations of nicotine retarded the clot-formation property of thrombin. By decreasing the nicotine concentration in the clotting mixture, the clotting time of thrombin was accelerated. Nicotine also modified the clot formation property of plasma or fibrinogen to thrombin, and this effect was dose dependent. From this experimental evidence, it is suggested that nicotine does alter the clot-forming properties of thrombin on fibrinogen.
D000470:Alkaloids / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D004357:Drug Synergism; D005340:Fibrinogen / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D009538:Nicotine / Q000494:pharmacology*; D010947:Plants, Toxic; D013917:Thrombin / Q000494:pharmacology*; D013997:Time Factors; D014026:Nicotiana
D000470:Alkaloids; D009538:Nicotine; D005340:Fibrinogen; D013917:Thrombin