Rapidly labeled intermediates in DNA replication in higher plants.
In detached Vicia embryos, the incorporation of [3-H] thymidine into DNA starts at about 25 h after the beginning of imbibition and reaches maximum at about 33 h. The DNA synthesized during the first replication phase was extracted. Alkaline sucrose density-gradient analyses of the DNA indicated the occurrence of several short pieces of rapidly labeled DNA having sedimentation values of approx. 10 S and 14 S, after a pulse for 5 to 10 min. Prolonged labeling and chase incubation led to a shift of the shorter fragments to longer ones of 19 S and 22 S or more, thus indicating the nature of intermediates during DNA replication of these short fragments.
D002499:Centrifugation, Density Gradient; D004247:DNA / Q000096:biosynthesis* / Q000302:isolation & purification; D004261:DNA Replication; D007700:Kinetics; D010944:Plants / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D004247:DNA; D013936:Thymidine