Fluorescence reactions of fluorescamine with levodopa and its derivatives: fluorescence assay of 3-methoxy-4-hydroxyphenylalanine in levodopa dosage forms.
A simple fluorometric method for the quantitation of 3-methoxy-4-hydroxyphenylalanine, either alone or in the presence of levodopa, is presented. Fluorescence is developed by reaction with fluorescamine. The interactions of this reagent with levodopa and a number of its derivatives under various experimental conditions were studied. Negligible fluorescence was obtained with levodopa, dopamine, levodopa benzyl ester, and 6-hydroxydopamine.
D055598:Chemical Phenomena; D002621:Chemistry; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives; D004298:Dopamine / Q000032:analysis; D005450:Fluorescamine; D005453:Fluorescence; D007980:Levodopa / Q000031:analogs & derivatives; D010084:Oxidation-Reduction; D010649:Phenylalanine / Q000031:analogs & derivatives*; D013056:Spectrophotometry, Ultraviolet; D013141:Spiro Compounds
D013141:Spiro Compounds; D005450:Fluorescamine; D007980:Levodopa; D010649:Phenylalanine; D004295:Dihydroxyphenylalanine; D004298:Dopamine