Constituents of Cannabis sativa L. VIII: Possible biological application of a new method to separate cannabidiol and cannabichromene.
Synthetic and naturally occurring cannabidiol and cannabichromene were distinctly separated without derivation by GLC using a 6% OV-1 column; an artifact of cannabichromene, cannabicyclol, was separated from (minus)-delta9-trans-tetrahydrocanna-bivarin. This procedure is versatile and applicable for the quantitation of Cannabis containing both cannabidiol and cannabichromene. Biological interaction among (minus)-delta9-trans-tetrhydrocanabinol, cannabichromene, and other cannabinoids in natural Cannabis preparations can now be studied. In the phenyl methyl silicone polymer series, cannabidiol precedes ca-nabichromene on columns containing below a 50% phenyl-to-methyl ratio. Columns containing below a 50% phenyl-to-methyl ratio. Columns containing a 50:50 or greater ratio of phenyl to methyl reverse the separation order with cannabichromene preceding cannabidiol.
D001578:Benzopyrans / Q000302:isolation & purification*; D002185:Cannabidiol / Q000302:isolation & purification*; D002186:Cannabinoids; D002188:Cannabis / Q000032:analysis* / Q000302:isolation & purification*; D002849:Chromatography, Gas; D013058:Mass Spectrometry; D008722:Methods; D013696:Temperature
D001578:Benzopyrans; D002186:Cannabinoids; D002185:Cannabidiol; C010695:cannabichromene