Deoxyribonucleic acid metabolism and nuclear division during spore germination in Fusarium oxysporum.
Ungerminated microconidia of Fusarium oxysporum have a mean cell DNA content of 0-134 times 10--12 g/cell with a guanine-plus-cytosine composition (%GC) of 50-75%. During germination, the first dry weight increase of the spore population was defected after 3 h incubation and the first germ tube appeared after 4 h. The total DNA of the culture sharply increased after 5 h, followed by a pause at 6 h. At this time the DNA content per nucleus was maximal and the first nuclear divisions were detected.   auses in the rise of total DNA of the culture and in the [14C]adenine incorporation pattern suggest that there is partial synchrony in DNA synthesis at the beginning of incubation. This is also supported by the fact that until 8 h, only hyphae with 1, 2 and 4 nucleic were observed. [14C]adenine incorporation into DNA averaged 2-68% of the total taken up in 10 h incubation.
D000225:Adenine / Q000378:metabolism; D002455:Cell Division; D002467:Cell Nucleus / Q000032:analysis; D004247:DNA / Q000096:biosynthesis*; D005670:Fusarium / Q000254:growth & development* / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D013172:Spores, Fungal / Q000032:analysis / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors
D004247:DNA; D000225:Adenine