[Microtiter agglutination test for salmonella and brucella antibodies (author's transl)].
To compare the tube agglutination test and the microtiter plate agglutination procedure, 207 human sera were tested for agglutinins to Salmonella and 267 sera for agglutinins to Brucella by both methods. A positive reaction in the microtiter test was indicated by a smooth or irregular mat shape at the bottom of each well in the microtiter plate, whereas a negative reaction was shown by a settling of the antigen to a button shape. As can be seen in table 1, identical titer or only one dilution difference between both tests were found in more than 93% of the samples tested. The plate test was easier to perform and saved time, space, antigen and serum. The microtiter-agglutination technique can, therefore, be recommended for the routine laboratory for serology.
D000372:Agglutination Tests / Q000379:methods*; D000373:Agglutinins / Q000032:analysis; D000907:Antibodies, Bacterial / Q000032:analysis*; D002002:Brucella / Q000276:immunology*; D006801:Humans; D012475:Salmonella / Q000276:immunology*; D012698:Serologic Tests
D000373:Agglutinins; D000907:Antibodies, Bacterial
Vergleich von Röhrchen- und Mikrotiter-Agglutination zum Nachweis von Antikörpern gegen Salmonellen und Brucellen