Role of Mn2+ in the reaction of polynucleotide phosphorylase with 2'-O-methylated substrates.
Polymerization of 2'-O-methylcytidine-5'-diphosphate (CmDP) with polynucleotide phosphorylase in the presence of Mn2+ proceeds with 65% yield after 72 h, and in the presence of Mg2+ the yield does not exceed 10%. Phosphorolysis of poly 2'-O-methylcytidylic acid and poly 2'-O-methyluridylic acid, as well as exchange of the beta-phosphate group of CmDP in the presence of Mn2+ and Mg2+, proceed with a yield of only a few percent. A possible mechanism of Mn2+ action on CmDP polymerization is discussed.
D055598:Chemical Phenomena; D002621:Chemistry; D003565:Cytidine Diphosphate / Q000031:analogs & derivatives; D003597:Cytosine Nucleotides / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology*; D008745:Methylation; D010710:Phosphates / Q000378:metabolism; D011072:Poly U / Q000031:analogs & derivatives / Q000378:metabolism; D011108:Polymers; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism*; D013329:Structure-Activity Relationship
D003597:Cytosine Nucleotides; D010710:Phosphates; D011108:Polymers; D011072:Poly U; C010485:2'-O-methylcytidine-5'-diphosphate; D008345:Manganese; D003565:Cytidine Diphosphate; D011117:Polyribonucleotide Nucleotidyltransferase; D008274:Magnesium