The intracellular localization and stability of the dihydrofolate synthetase in pea seedlings.
The amount of folate compounds rapidly increased during germination of the pea. An especially high increase was observed 2 days after sowing, with a continouously increasing content during germination. Dihydrofolate synthetase [EC 6.3.2.12] activity also increased with the increase in the amount of folate compounds, and the maximum activity was observed at 6 days after sowing in the dark and at 8 days after sowing in the light. The dihydrofolate synthetase was localized mostly in the mitochondrial fraction and it was easily extracted from the cell particles by osmotic shock. A similar localization was also observed in spinach leaves. The enzyme which was extracted from the isolated mitochondria was relatively stable im comparison with that extracted from whole cells.
D002736:Chloroplasts / Q000201:enzymology; D003624:Darkness; D004355:Drug Stability; D005492:Folic Acid / Q000031:analogs & derivatives / Q000096:biosynthesis; D005971:Glutamates; D008027:Light; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D010453:Peptide Synthases / Q000378:metabolism*; D063245:Plant Development; D010944:Plants / Q000201:enzymology*; D011622:Pterins; D012639:Seeds / Q000201:enzymology; D013347:Subcellular Fractions / Q000201:enzymology
D005971:Glutamates; D011622:Pterins; D005492:Folic Acid; D010453:Peptide Synthases