Isozymes of alpha-amylase in human urine.
Some physicochemical properties of crystalline alpha-amylase [EC 3.2.1.1] isolated from normal human urine were investigated. A crystalline preparation of the enzyme was homogeneous on velocity sedimentation, gel filtration, and sodium dodecyl sulfate disc electrophoresis, and its molecular weight was estimated to be 4.5 X 10-4. However, electrophoretic analyses revealed that the crystalline alpha-amylase consisted of at least five isozymes. The implications of the experimental results are discussed.
D000681:Amylases / Q000302:isolation & purification / Q000652:urine*; D002850:Chromatography, Gel; D003460:Crystallization; D004589:Electrophoresis, Disc; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification / Q000652:urine*; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation
D007527:Isoenzymes; D000681:Amylases