Further studies on the isolation of ribosomes from normal and methylcholanthrene-treated mouse epidermis.
Ribosomes from normal or hyperplastic mouse epidermis have been isolated from the postmitochondrial supernatant and from the entire cytoplasm using either the postnuclear supernatant technique or the Redman technique which permits isolation of ribosomes separately from the nuclear, mitochondrial, and postmitochondrial fractions. In contrast to liver, the yields of ribosomes from skin are similar irrespective of what technique is used. Increasing the centrifugation force from 105,000 gav to 253,000 gav results in highly significant increases in the yields of ribosomes, from both normal and hyperplastic epidermis.
D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000648:ultrastructure; D005260:Female; D006965:Hyperplasia / Q000473:pathology*; D008099:Liver / Q000648:ultrastructure; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008928:Mitochondria / Q000648:ultrastructure; D012335:RNA, Ribosomal / Q000302:isolation & purification* / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects; D012867:Skin / Q000187:drug effects / Q000648:ultrastructure*
D012335:RNA, Ribosomal; D008748:Methylcholanthrene