Phosphate uptake into organic compounds in skeletal muscle.
Isolated intact frog muscles were incubated in 32-P-labelled Ringer's solution for various periods of time (30 s-20 h). Labelled compounds were isolated from TCA, methanol-chloroform-water, and water extracts of muscle. Hexosephosphates, phosphocreatine, phosphoenolphyruvate, alpha-glycerol phosphate, adenosine triphosphate, and inorganic phosphate were identified after 30 s, and 4 h incubation. Much more labelling was found after 20 h. The incorporation of 32-P in 30 s into organic phosphate compounds, such as alpha-glycerol phosphate and ATP, showed that immediate esterification of Pi occured on, or just inside, the sarcolemma.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001001:Anura; D001345:Autoradiography; D005994:Glycerophosphates / Q000378:metabolism; D066298:In Vitro Techniques; D008055:Lipids / Q000032:analysis; D009132:Muscles / Q000032:analysis / Q000378:metabolism*; D010710:Phosphates / Q000032:analysis / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D011506:Proteins / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D005994:Glycerophosphates; D008055:Lipids; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D011506:Proteins; D000255:Adenosine Triphosphate