The excision of pyrimidine dimers from the DNA of mutant and wild-type strains of Ustilago.
UV-induced pyrimidine dimers were excised from the DNA of wild-type and four mutant strains of Ustilago maydis. Excision was partially dose dependent. The kinetics of excision differed in recombination deficient strains (rec 1 and rec 2) from those found in a recombination proficient radiation-sensitive strain (uvs 3). At fluences above 100 J-m-2 excision was saturated in uvs 3 but not in rec 1 or rec 2. Fluences above 300 J-m-2 started to saturate excision in wild-type. pol1-1, a temperature-sensitive DNA polymerase mutant, was excision proficient at both the permissive (22 degree) and restrictive (32 degree) temperatures. Wild-type cells were observed to excise CC before CT or TT in high dose experiments.
D001487:Basidiomycota / Q000528:radiation effects*; D004247:DNA / Q000528:radiation effects*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D007700:Kinetics; D008027:Light; D009154:Mutation; D011831:Radiation Genetics; D011995:Recombination, Genetic; D014466:Ultraviolet Rays; D014588:Ustilago / Q000201:enzymology / Q000528:radiation effects*
D004247:DNA; D004254:DNA Nucleotidyltransferases