Endotoxin, thrombin, and the Limulus amebocyte lysate test.
The Limulus amebocyte lysate, a proteinaceous composite isolated from the hemolymph cells of the horseshoe crab (Limulus polyphemus) is sensitive to picogram quantities of Gram-negative bacterial lipopolysaccharides. However, a controversy currently exists as to whether the Limulus amebocyte lysate is specifically sensitive to Gram-negative bacterial endotoxins as a result of a recent report that the blood coagulation protease, thrombin, can mimic endotoxins in the Limulus amebocyte lysate test. Experiments including those employing two highly purified fractions isolated from the Limulus lystae have provided us with evidence that thrombin per se is unable to mimic endotoxin.
D000818:Animals; D001097:Arachnida / Q000187:drug effects*; D001681:Biological Assay / Q000379:methods; D002417:Cattle; D002725:Chloroform / Q000494:pharmacology; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004586:Electrophoresis; D004731:Endotoxins / Q000032:analysis / Q000494:pharmacology*; D006458:Hemolymph / Q000187:drug effects*; D006358:Hot Temperature; D066298:In Vitro Techniques; D007531:Isoflurophate / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology*
D004731:Endotoxins; D007531:Isoflurophate; D002725:Chloroform; D013917:Thrombin