Simple radio-immunoassay for the measurement of human and rat IgE levels by ammonium sulfate precipitation.
A simple assay for quantitating IgE levels has been developed based on the observation that antibody-bound IgE is insoluble in 33 percent saturated (NH-4)2-SO-4 whereas unbound IgE is not. The method has been applied to human and rat IgE. Some preliminary results on IgE levels in parasite-injected rats are presented.
D000645:Ammonium Sulfate; D000818:Animals; D006801:Humans; D007106:Immune Sera; D007073:Immunoglobulin E / Q000032:analysis*; D007141:Immunoglobulin Fc Fragments; D007457:Iodine Radioisotopes; D009194:Myeloma Proteins; D009559:Nippostrongylus / Q000276:immunology; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats
D007106:Immune Sera; D007141:Immunoglobulin Fc Fragments; D007457:Iodine Radioisotopes; D009194:Myeloma Proteins; D007073:Immunoglobulin E; D000645:Ammonium Sulfate