Transfer of serine into polypeptides and myosin by chromatographic species of seryl-transfer ribonucleic acid.
The efficiencies of two chromatographic species of [3-H]seryl-tRNA, namely peaks I and II, in cell-free amino acid incorporation were investigated. The maximum yield of polypeptide seems to be the same for the reaction mixtures containing either peak I or peak II, suggesting that the efficiency of both peaks in total protein synthesis is the same. The efficiency of transfer of serine into myosin heavy subunit (myosin H) by peaks I and II was also investigated. Peak II of [3-H]seryl-tRNA transfers three times as much serine into myosin H as peak I.
D000604:Amino Acyl-tRNA Synthetases; D000818:Animals; D002474:Cell-Free System; D002642:Chick Embryo; D002848:Chromatography, DEAE-Cellulose; D009132:Muscles / Q000378:metabolism; D009218:Myosins / Q000096:biosynthesis*; D010452:Peptide Biosynthesis; D011132:Polyribosomes / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism*; D012694:Serine / Q000378:metabolism*
D012694:Serine; D012343:RNA, Transfer; D009218:Myosins; D000604:Amino Acyl-tRNA Synthetases