[DNA-dependent RNA polymerase from the nuclei of the spleen of white rats].
DNA-dependent RNA polymerase was isolated from rat spleen cell nuclei and was identified as A and B RNA polymerases by data on DEAE- and P-cellulose ionic exchange chromatography and on concentration dependency on bivalent ions and (NH4)2SO4. Two forms of the enzyme differed from each other in the activity in RNA synthesizing system, and their activity was completely inhibited by actinomycin, DNase and RNase.
D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology*; D002712:Chlorides / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D004247:DNA / Q000494:pharmacology*; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification*; D003609:Dactinomycin / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008345:Manganese / Q000494:pharmacology; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology; D013053:Spectrophotometry; D013154:Spleen / Q000166:cytology*
D002712:Chlorides; D003609:Dactinomycin; D008345:Manganese; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D003851:Deoxyribonucleases; D012260:Ribonucleases; D008274:Magnesium; D000645:Ammonium Sulfate; D012293:Rifampin
DNK-zavisimaia RNK-polimeraza iz iader selezenki belykh krys