Rapid screening method for aflatoxins and zearalenone in corn.
The methanol-water extraction system used in AOAC Method II for aflatoxins extracts both the aflatoxins and zearalenone from corn. Using this methanol-water extraction system as a base, a rapid screening procedure has been developed for these mycotoxins. The methanol-water extract is defatted with hexane and the pigments are precipitated with copper carbonate. The aflatoxins and zearalenone are subsequently extracted into chloroform and are then detected by half-plate TLC. An elapsed time of about 1 hr is required to analyze 1 sample. The sensitivity of the method is about 2 mu-g/kg for aflatoxin B-1 and 100 mu-g/kg for zearalenone.
D000348:Aflatoxins / Q000032:analysis; D007783:Lactones / Q000032:analysis*; D008722:Methods; D012997:Solvents; D003313:Zea mays / Q000032:analysis*; D015025:Zearalenone / Q000032:analysis*
D000348:Aflatoxins; D007783:Lactones; D012997:Solvents; D015025:Zearalenone