Unique cathepsin D-type proteases in rat thoracic duct lymphocytes and in rat lymphoid tissues.
Two unique cathepsin D-type proteases apparently present only in rat thoracic duct lymphocytes and in rat lymphoid tissues are described. One, termed H enzyme, has an apparent molecular weight of similar to95,000; the other, termed L enzyme, has an apparent molecular weight of similar to45,000, in common with that of most cathepsins D from other tissues and species. Both enzymes differ from cathepsin D, however, by a considerably greater sensitivity to inhibition by pepstatin and by a smaller degree of inhibition by an antiserum which inhibits rat liver cathepsin D. H enzyme is converted to L enzyme by treatment with beta-mercaptoethanol; the relationship between the two enzymes remains unknown. H and L enzyme have been detected in rat lymphoid tissues and in mouse spleen, but they are not present in other rat tissues (liver, kidney, adrenals), rabbit tissues, calf thymus, bovine spleen, or human tonsils. As measured on acid-denatured bovine hemoglobin as substrate, both enzymes have pH activity curves identical with that of rat liver cathepsin D, with optimal activity at pH 3.6. Activity on human serum albumin is much less and also shows an optimum at pH 3.6; hence, neither enzyme has the properties of cathepsin E. Thiol-reactive inhibitiors have no effect on the activity of H and L enzyme; thus they do not belong to the B group of cathepsins. Additional information, discussed in this paper, leads us to conclude that partially purified H and L enzymes are cathepsin D-type proteases.
D000311:Adrenal Glands / Q000201:enzymology; D000818:Animals; D000937:Antigen-Antibody Reactions; D002403:Cathepsins / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D006801:Humans; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology; D008198:Lymph Nodes / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology*; D008221:Lymphoid Tissue / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D008970:Molecular Weight; D009928:Organ Specificity; D014066:Palatine Tonsil / Q000201:enzymology; D011817:Rabbits / Q000276:immunology; D051381:Rats; D013154:Spleen / Q000201:enzymology; D013897:Thoracic Duct / Q000201:enzymology*; D013950:Thymus Gland / Q000201:enzymology
D007527:Isoenzymes; D002403:Cathepsins