Isolation of DNA polymerase gamma from an adenovirus 2 DNA replication complex.
The major DNA polymerase in a nuclear membrane complex that is capable of synthesizing viral DNA sequences in vitro has been purified about 900-fold from adenovirus 2-infected KB cells. The enzyme was characterized as belonging to the class of mammalian DNA polymerases (DNA polymerase gamma) that can utilize poly(A) with oligo(dT) as template primer.
D000256:Adenoviridae / Q000378:metabolism*; D002277:Carcinoma; D002458:Cell Fractionation; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D006801:Humans; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D009062:Mouth Neoplasms; D011092:Polyethylene Glycols; D011119:Polynucleotides / Q000378:metabolism; D013698:Templates, Genetic
D004279:DNA, Viral; D011119:Polynucleotides; D011092:Polyethylene Glycols; D008345:Manganese; D004254:DNA Nucleotidyltransferases