Cellulose acetate electrophoresis of creatine phosphokinase isoenzymes in the diagnosis of myocardial infarction.
A method for rapid separation and visualization of creatine phosphokinase (EC 2.7.3.2 ATP: creatine phosphotransferase) isoenzymes on cellulose acetate has been developed. The technic uses the Beckman microzone electrophoresis system, and is applicable to other cellulose acetate systems. Incubation is with a commercially available substrate solution, and the isoenzyme bands are visualized by fluorescence using a long-wavelength ultraviolet light. An intermediate migrating isoenzyme band (CPK-MB) was detected in sera of 67 of 68 patients with myocardial infarction, and was not present in patients with other disorders that might clinically be confused with myocardial infarction.
D000787:Angina Pectoris / Q000201:enzymology; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001921:Brain / Q000201:enzymology; D002764:Cholecystitis / Q000201:enzymology; D003402:Creatine Kinase / Q000032:analysis* / Q000097:blood; D004562:Electrocardiography; D004588:Electrophoresis, Cellulose Acetate; D006333:Heart Failure / Q000201:enzymology; D006801:Humans; D007037:Hypothyroidism / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D009132:Muscles / Q000201:enzymology; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D011655:Pulmonary Embolism / Q000201:enzymology; D013997:Time Factors
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase