Specificity of SLE serum antibody for single-stranded and double-stranded DNA configuration.
The solid phase immunoassay for the measurement of serum antibody to double and single-stranded DNA is described. The technique allows a more accurate definition of the antibody specificity of SLE sera as defined by inhibition techniques. The clinical usefulness of immunologic assays in the management of patients with SLE is described, including measurement of urine light chain protein concentration. A core discussion is provided to illustrate how such a panel of tests is used in management.
D000293:Adolescent; D000645:Ammonium Sulfate; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000276:immunology; D011232:Chemical Precipitation; D002699:Chlorambucil / Q000627:therapeutic use; D003165:Complement System Proteins / Q000378:metabolism; D004247:DNA / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000276:immunology*; D005921:Glomerulonephritis / Q000209:etiology / Q000276:immunology; D006801:Humans; D007118:Immunoassay; D007128:Immunoglobulin Fragments / Q000652:urine; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000276:immunology* / Q000652:urine; D008297:Male; D011241:Prednisone / Q000627:therapeutic use; D011507:Proteinuria / Q000276:immunology
D004277:DNA, Single-Stranded; D007128:Immunoglobulin Fragments; D002699:Chlorambucil; D003165:Complement System Proteins; D004247:DNA; D000645:Ammonium Sulfate; D011241:Prednisone