[Effect of 0.005M CaCl-2 and 0.14M NaCl on the yield and extractibility of histones from rat liver nuclei].
The release and extraction of histones from the nuclei of rat liver under the influence of 0.005 M CaCl2 and 0.14 M NaCl was investigated. A considerable loss of nuclear histones, especially lysine-rich ones, was shown biochemically and cytophotometrically. The preliminary incubation of the nuclei in the respective salt solutions resulted in a more complete extraction of histones. This may be explained by a weaker binding capacity of histones to DNA under the latter conditions. Electrophoresis in polyacrylamid gel showed an increased mobility of histone fractions removed from the nuclei by incubation in the salt solutions.
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002467:Cell Nucleus / Q000032:analysis* / Q000187:drug effects; D006657:Histones / Q000302:isolation & purification*; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis* / Q000187:drug effects; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology
D006657:Histones; D012965:Sodium Chloride; D002122:Calcium Chloride
Vliianie 0.005M CaCl-2 i 0.14M NaCl na vykhod i ékstragiruemost' gistonov iz iader pecheni krys