Analytical and comparative chromatographic maps of oligosaccharides produced by acetolysis and partial acid hydrolysis of glycoprotids.
The authors describe a chromatographic mapping procedure of oligosaccharides present in acetolysates and partial acid hydrolysates of glycopeptides or glycoproteins. Oligosaccharides are first fractionated on charcoal-Celite columns and then identified by paper chromatography. This procedure is sensitive and reproducible and allows to compare the structure of N-glycans and of glycoproteins from various sources.
D000085:Acetates; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography / Q000379:methods; D002854:Chromatography, Paper; D006023:Glycoproteins / Q000032:analysis*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000032:analysis*; D014168:Transferrin / Q000032:analysis
D000085:Acetates; D006023:Glycoproteins; D009844:Oligosaccharides; D014168:Transferrin