[Creatine induction of creatine kinase synthesis in a developing monolayer culture of muscle cells].
Creatine action on the activity of creatine kinase (ATP: creatine-phosphotransferase; EC 2.7.3.2) and the content of water-soluble proteins in the developing monolayer culture of chick myoblasts are studied. Creatine at concentrations of 1.9-10- minus 3-3.8-10- minus 3 M is shown to increase reliably the creatine kinase activity by 1,1--2,9 times and to reduct considerably the content of water-soluble proteins. Lower concentrations of creatine (3.8-10- minus 5 M) also increased the creatine kinase activity but did not change the contents of water-soluble proteins. The creatine effect was maximal at the period preceding the termination of tissue cells differentiation. In the course of the combined effect of both actinomycin D (50 mcg/plate) and creatine (3.8-10- minus 3 M) the creatine kinase activity was much higher than that in the presence of actinomycin D alone which considerably reduced the enzyme activity as well as the contents of water-soluble proteins.
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003401:Creatine / Q000494:pharmacology*; D003402:Creatine Kinase / Q000096:biosynthesis*; D003470:Culture Media; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004790:Enzyme Induction / Q000187:drug effects; D009124:Muscle Proteins / Q000032:analysis; D009132:Muscles / Q000166:cytology*; D013997:Time Factors
D003470:Culture Media; D009124:Muscle Proteins; D003609:Dactinomycin; D003402:Creatine Kinase; D003401:Creatine
Induktsiia kreatinom sinteza kreatinkinazy v razvivaiushcheĭsia monosloĭnoĭ kul'ture myshechnykh kletok