Effects of short term ether and pentobarbital anesthesia on bone and cartilage metabolism.
The effects of stress due to brief (4--5 min) ether and pentobarbital anesthesia vs. decapitation on assays of seven enzymes in homogenates of synovium, articular and epiphyseal cartilage, and metaphyseal and cortical bone were compared. Etherization caused twofold changes in synovial and articular cartilage G-6-PDH, LDH, CPK, glutamic DH, and ICDH based on tissue dry weight and DNA content. Pentobarbital anesthesia produced only slightly lower activities, per gram DNA, of LDH, acid phosphatase, and glutamic-DH in cortical bone. Epiphyseal cartilage metabolism was unaffected by either mode of anesthesia. No difference could be detected between levels of enzyme activities of the several tissues taken from rats that had been decapitated or anesthetized with pentobarbital. The changes in enzyme activities suggested that pentobarbital was non-stressful and appropriate to metabolic studies in the skeleton.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000758:Anesthesia; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects* / Q000201:enzymology; D002356:Cartilage / Q000187:drug effects* / Q000201:enzymology; D003402:Creatine Kinase / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004986:Ether / Q000494:pharmacology*; D005019:Ethyl Ethers / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010424:Pentobarbital / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D051381:Rats
D005019:Ethyl Ethers; D011506:Proteins; D004986:Ether; D004247:DNA; D000429:Alcohol Oxidoreductases; D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005969:Glutamate Dehydrogenase; D003402:Creatine Kinase; D010424:Pentobarbital