Activity of eight enzymes of chicken seminal plasma in the eluent from agarose gel chromatography.
Chromatography of seminal plasma from fresh, untreated chicken semen on Bio-Rad A1.5m agarose gel yielded five major peaks of ultraviolet absorbancy at 280 nm.  Two peaks with 280/260 absorbancy ratios less than unity suggested the presence of free nucleotides.  Enzyme assays on the eluent fractions resulted in substantial single peaks of lactic dehydrogenase, glutamic oxaloacetic transaminase, phosphohexose isomerase, acid phosphatase and alkaline phosphatase activity.  Acetylcholinesterase and aminopeptidase assays produced multiple peaks of activity.  No trypsin-like enzyme activity was detected, suggesting the presence of a seminal plasma trypsin-like enzyme inhibitor.  Molecular weight estimates were obtained for all enzyme activity peaks.
D000110:Acetylcholinesterase / Q000302:isolation & purification; D000135:Acid Phosphatase / Q000302:isolation & purification; D000469:Alkaline Phosphatase / Q000302:isolation & purification; D000626:Aminopeptidases / Q000302:isolation & purification; D000818:Animals; D001219:Aspartate Aminotransferases / Q000302:isolation & purification; D002645:Chickens / Q000378:metabolism*; D002847:Chromatography, Agarose; D005956:Glucose-6-Phosphate Isomerase / Q000302:isolation & purification; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification; D008297:Male; D008970:Molecular Weight; D009711:Nucleotides / Q000302:isolation & purification; D012661:Semen / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet
D009711:Nucleotides; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000110:Acetylcholinesterase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000626:Aminopeptidases; D005956:Glucose-6-Phosphate Isomerase