Molecular weight of the acetylcholine receptors of electric organs and the effect of Triton X-100.
Studies are reported on the influence of Triton X-100 on the molecular weight and functional properties of the acetylcholine receptor. Results are presented principally for receptors purified from Torpedo californica and Torpedo marmorata with a limited number of observations on the receptor from Electrophorus electricus. In equilibrium dialysis measurements Trito, X-100 greatly reduced acetylcholine binding to the high affinity sites of the receptor from T. californica, but had only a small effect on the sites of lower affinity. Sedimentation equilibrium experiments on receptor in the absence of added Triton X-100 revealed average apparent molecular weight values of 510,000 for receptor from T. californica and 665,000 for T. marmorata. Under those conditions 0.113 mg of residual Triton X-100 were found per mg of protein as determined by using 3H-labeled Triton X-100. The sedimentation data indicated the presence of more than one molecular species, involving a unit with an apparent molecular weight of 330,000 and higher aggregates. Upon addition of Triton X-100, the higher aggregates were reduced, and above 0.1 percent Triton X-100 the 330,000 unit was the principal component present for receptor from all three species examined. Various structural models are considered in the light of this value, the polypeptide size from Na dodecyl sulfate-gel electrophoresis, and the protomer size determined by the molecular weight of an acetylcholine binding site.
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D003956:Dialysis; D004557:Electric Organ / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D007700:Kinetics; D008433:Mathematics; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011092:Polyethylene Glycols; D011485:Protein Binding; D000644:Quaternary Ammonium Compounds; D011950:Receptors, Cholinergic; D013045:Species Specificity
D009419:Nerve Tissue Proteins; D000644:Quaternary Ammonium Compounds; D011950:Receptors, Cholinergic; D011092:Polyethylene Glycols; D000109:Acetylcholine