Gas chromatographic determination of beta-phenethylbiguanide and its metabolite p-hydroxy-beta-phenethylbiguanide in serum and urine.
A gas chromatographic method is presented for the detection of beta-phenethylbiguanide (PEBG) and its metabolite, p-hydroxy-beta-phenethylbiguanide (p-OHPEBG). The procedure is applicable for the determination of the drug and its metabolite in the serum and urine of rats. The detection limit is 0.2 mug PEBG and 0.5 mug p-OH PEBG per ml of serum or urine. A time course study of blood concentration and elimination rate following intraperitoneal injection of 100 mg/kg of PEBG to normal rats was performed. Beta-PEBG was found to be present in the blood and the urine, p-OH PEBG was only detected in the urine. Twenty-four hours following intraperitoneal injection, the urine contained 32% of the administered dose, 20% as unaltered PEBG and 12% as p-OH PEBG.
D000818:Animals; D002849:Chromatography, Gas; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D008722:Methods; D010629:Phenformin / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000378:metabolism / Q000652:urine; D051381:Rats; D013997:Time Factors
D010629:Phenformin