New method for the isolation and crystallization of human liver alkaline phosphatase.
A new and simple method for the purification of human liver alkaline phosphatase using butanol extraction, acetone and ammonium sulfate precipitations, anion exchange chromatography and gel filtration is described.
D000096:Acetone; D000469:Alkaline Phosphatase / Q000302:isolation & purification*; D000645:Ammonium Sulfate; D000440:Butanols; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008722:Methods
D000440:Butanols; D000096:Acetone; D000469:Alkaline Phosphatase; D000645:Ammonium Sulfate