Standardization of stinging insect extracts.
The measurement of IgE Ab to insects (bee etc.) can be accomplished using RAST-discs. This prevents the non-specific wheal and flare produced when skin tests in higher concentrations of either whole body or venom are used. It will also eliminate the possibility of a serious systemic reaction by direct skin testing. The in vitro neutralization of IgE antibody by pure fractions and or components employing the RAST disc method may well be a useful tool for standardization of allergens.
D000485:Allergens / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D001516:Bees / Q000276:immunology; D005069:Evaluation Studies as Topic; D006801:Humans; D006927:Hymenoptera / Q000276:immunology*; D007073:Immunoglobulin E / Q000032:analysis*; D008722:Methods; D008832:Microchemistry; D011863:Radioimmunoassay; D012882:Skin Tests; D014688:Venoms / Q000032:analysis
D000485:Allergens; D000906:Antibodies; D014688:Venoms; D007073:Immunoglobulin E