Automated spectrophotometric assay of cefazolin.
An automated, stability-indicating, UV spectrophotometric assay for cefazolin is presented. The method employs a reaction with hydroxylamine and derives its stability-indicating power through comparison of reacted and unreacted aliquots of the sample. A double-probe sampling procedure is used. Good agreement with microbiological assays is obtained, and the coefficient of variation is about 1%.
D001322:Autoanalysis / Q000295:instrumentation; D002437:Cefazolin / Q000032:analysis*; D002511:Cephalosporins / Q000032:analysis*; D006898:Hydroxylamines / Q000032:analysis; D008722:Methods; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation
D002511:Cephalosporins; D006898:Hydroxylamines; D002437:Cefazolin