Liquid ion exchangers in paper chromatography of steroidal glucosiduronic acids, glucosiduronic esters and free steroids. Influence of concentration of exchanger and counterion.
The chromatographic mobility of steroidal glucosiduronic acids on paper in chloroform-formamide increases as the concentration of ion exchanger in the chloroform phase increases; mobility decreases as the concentration of counterion in formamide increases. Mobility of glucosiduronic esters and of hydroxylated free steroids increases with an increase in concentration of exchanger; small changes in concentration of counterion in the stationary phase do not alter the migration of these nonionizable compounds. Data are presented which suggest that partition of the glucosiduronic acids between the two phases occurs predominantly by an ion-exchange process and that hydrogen bonding plays a secondary role. Partition of the glucosiduronic esters and hydroxylated free steroids appears to occur primarily by a hydrogen-bonding process.
D002784:Cholesterol / Q000031:analogs & derivatives; D002845:Chromatography / Q000379:methods*; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D005965:Glucuronates / Q000032:analysis*; D006914:Hydroxysteroids / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D013256:Steroids / Q000032:analysis*; D013261:Sterols / Q000032:analysis
D005965:Glucuronates; D006914:Hydroxysteroids; D000644:Quaternary Ammonium Compounds; D013256:Steroids; D013261:Sterols; D002784:Cholesterol