Structure of N-terminal cyanogen bromide fragment of human plasma albumin.
The complete amino acid sequence of 87 residues of cyanogen bromide fragment CB1 (Asp), the N-terminal fragment of human plasma albumine molecule, has been established. The sequence was determined from the characterization of all tryptic peptides and of chymotryptic arginine-containing peptides in the fragment digested. Overlaps were obtained by tryptic and chymotryptic cleavage of the maleylated S-sulfo derivative of fragment CB1(Asp). Residue 34 is the only cysteine residue in the albumin molecule and it was determined in the form of S-carboxymethyl-cysteine. Edman and dansyl-Edman degradation were used for the sequential analysis.
D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D003619:Dansyl Compounds / Q000032:analysis; D004586:Electrophoresis; D006801:Humans; D006868:Hydrolysis; D007460:Iodoacetamide / Q000494:pharmacology; D008298:Maleates / Q000494:pharmacology; D010446:Peptide Fragments / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis*; D013447:Sulfites / Q000494:pharmacology; D013820:Thermolysin; D014357:Trypsin
D003619:Dansyl Compounds; D008298:Maleates; D010446:Peptide Fragments; D012709:Serum Albumin; D013447:Sulfites; D002918:Chymotrypsin; D014357:Trypsin; D013820:Thermolysin; D003488:Cyanogen Bromide; D007460:Iodoacetamide