Reinitiation of DNA synthesis in senescent human fibroblasts upon fusion with cells of unlimited growth potential.
Postreplicative, "senescent" human fibroblasts were fused to HeLa or to SV-40 transformed human fibroblasts with Sendai virus. DNA synthesis was reinitiated in senescent nuclei in a high proportion of the heterodikaryons. The [3H]thymidine labeling index of senescent fibroblast nuclei in heteropolykaryons was a function of the ratio of HeLa to senescent nuclei.
D002459:Cell Fusion; D002467:Cell Nucleus / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D005347:Fibroblasts / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D006822:Hybrid Cells; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D004247:DNA; D013936:Thymidine