Toxicity of antibiotics on cultured human skin fibroblasts.
Human diploid fibroblasts cultured in Dulbecco's Modified Eagle's medium (DME) were exposed to different concentrations of 15 antibiotics to determine the limiting toxic concentration. The number of cells surviving after antibiotic treatment was given as the index of toxicity. No visible chromosomal damage could be detected when half the maximal toxic concentration was applied. The maximum limiting concentration was found to be the same for both the preconfluent and postconfluent phases.
D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002512:Cephalothin / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D002842:Chromatids / Q000187:drug effects; D002869:Chromosome Aberrations; D003022:Clotrimazole / Q000494:pharmacology; D004917:Erythromycin / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D010118:Oxytetracycline / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D013420:Sulfamethoxazole / Q000494:pharmacology; D014295:Trimethoprim / Q000494:pharmacology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D010406:Penicillins; D004917:Erythromycin; D002701:Chloramphenicol; D014295:Trimethoprim; D003022:Clotrimazole; D013420:Sulfamethoxazole; D002512:Cephalothin; D010118:Oxytetracycline