Structural characteristics of native and enzymically formed dextran of S. sanguis ATCC 10558.
The structure of the native dextran produced by Streptococcus sanguis ATCC 10558 was analyzed by g.l.c.-m.s. of the methylated alditol acetates derived from the polymer. The results indicate that the polymer contains D-glucosyl residues substituted at C-6 or C-3, or both, as well as unsubstituted D-glucosyl residues. These data aially purified dextransucrase on sucrose. The proportion of D-glucosyl residues substituted at C-3 is diminished in this case. It is concluded that several enzymes are involved in the dextran synthesis.
D002474:Cell-Free System; D002849:Chromatography, Gas; D003911:Dextrans / Q000096:biosynthesis; D005947:Glucose / Q000032:analysis; D005964:Glucosyltransferases; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008757:Methylglucosides / Q000032:analysis; D008968:Molecular Conformation; D013291:Streptococcus / Q000032:analysis / Q000378:metabolism*
D003911:Dextrans; D008757:Methylglucosides; D005964:Glucosyltransferases; D005947:Glucose