A quantitative method for determination of aflatoxin B in roasted corn.
Roasting aflatoxin-contaminated corn will reduce toxin levels. A quantitative analysis for aflatoxin in roasted corn has been developed by modifying a cleanup technique for green coffee extracts approved as official first action by the AOAC. A chloroform extract is partially purified on a Florisil column, and thin layer chromatographic (TLC) plates are developed with methylene chloride-chloroform-isoamyl alcohol-formic acid (81+15+3+1). Recoveries average 101% and the sensitivity limit is 5 ppb aflatoxin B1. A 2-dimensional TLC procedure can also be used to separate the aflatoxins from background interferences.
D000348:Aflatoxins / Q000032:analysis*; D002855:Chromatography, Thin Layer; D003720:Densitometry; D005506:Food Contamination; D006358:Hot Temperature; D008722:Methods; D013997:Time Factors; D003313:Zea mays / Q000032:analysis*
D000348:Aflatoxins