Characterization and messenger activity of poly(A)-containing RNA from Chlorella.
Poly(A)-containing RNA was isolated by cellulose column chromatography from total RNA extracted from Chlorella fusca var. vacuolata 211/8p. RNA retained by the column was identified as poly(A)-containing RNA because it contained ribonuclease-resistant tracts, 25 to 55 nucleotides in length, from which not less than 80% of base was found to be adenine after acid hydrolysis. The base composition of poly(A)-containing RNA differed from that of RNA (largely ribosomal) which did not adsorb to cellulose, having a higher adenine content and a lower guanine content. Poly(A)-containing RNA was polydisperse including molecules with mobilities from 10S to 40S with a mean of about 20S. In an in vitro system derived from wheat-germ, protein synthesis was stimulated by adding poly(A)-containing RNA from Chlorella. Optimum conditions were established in this system with respect to the amount of poly(A)-containing RNA added and the concentration of KCl and Mg-2+. It is proposed that, in Chlorella, poly(A)-containing RNA includes cytoplasmic mRNA as has been shown for some other eucaryotic organisms.
D000227:Adenine Nucleotides / Q000032:analysis; D002499:Centrifugation, Density Gradient; D005591:Chemical Fractionation; D002708:Chlorella / Q000032:analysis*; D003596:Cytosine / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006147:Guanine / Q000032:analysis; D008274:Magnesium / Q000494:pharmacology; D011119:Polynucleotides / Q000032:analysis; D011189:Potassium Chloride / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D012260:Ribonucleases; D014498:Uracil / Q000032:analysis
D000227:Adenine Nucleotides; D011119:Polynucleotides; D012333:RNA, Messenger; D014498:Uracil; D006147:Guanine; D012313:RNA; D011189:Potassium Chloride; D003596:Cytosine; D012260:Ribonucleases; D008274:Magnesium