II. The influence of 17-beta-oestradiol on aminoacyl-tRNA synthetases from mouse uterus and mouse liver.
The activities of 17 aminoacyl-tRNA synthetases have been determined in liver and uterus preparations of mice. One group of mice were castrated and seven days later given 5 mug 17-beta-oestradiol in olive oil; a similar dose was given after 24 h. The animals were sacrificed one day later. A control group of mice which were also castrated, received olive oil without 17-beta-oestradiol. As the aminoacyl-tRNA synthetases may be found both in high molecular weight and low molecular weight forms, the forms present in the preparations are discussed. The activities of the aminoacyl-tRNA synthetases from uterus augmented under the influence of 17-beta-oestradiol, but to different degrees. The increase in the activities of isoleucyl- and prolyl-tRNA synthetases was not significant. In liver, only the activity of lysyl-tRNA synthetase augmented significantly.
D000596:Amino Acids / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000201:enzymology; D051379:Mice; D014599:Uterus / Q000201:enzymology
D000596:Amino Acids; D004958:Estradiol; D000604:Amino Acyl-tRNA Synthetases