A variant of hexokinase isoenzymes detected in ascites tumor cells.
In the hexokinase isoenzyme pattern on the cellulose acetate membrane electropherogram of the supernatant fraction of Ehrlich ascites tumor cells, a band was detected moving slower than of common Type I isoenzyme. This band was assumed to be due to the presence of a variant of the hexokinase isoenzymes in the cells, since such a band was not detected in the normal tissues, and a change in the intensity of the band in response to a sulfhydryl agent, etc., differed from that of the common Type I and II isoenzymes. The band was also found in AH-7974 and MH-134 ascites tumor cells, in spite of the difference in the species of the tumor-bearing animals and originating tissue.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D004586:Electrophoresis; D006593:Hexokinase / Q000302:isolation & purification*; D007527:Isoenzymes / Q000302:isolation & purification*; D008297:Male; D051379:Mice; D009374:Neoplasms, Experimental / Q000201:enzymology; D051381:Rats
D007527:Isoenzymes; D006593:Hexokinase