[Chromatography of nucleic acids, proteins and certain phages on granulated hydroxyapatite].
A modification of the classical method of hydroxyapatite synthesis is proposed. The essence of the modification is hydroxyapatite synthesis in the presence of an additional component silicic acid particles. The subsequent steps of the method are modified so, as to retain the intactness of crystals at all the stages of preparation and use of the adsorbent. The final product consists of large spherical agregates (200-250 mu in diameter) and contains about 1% of tightly bound silicic acid. It slightly differs from usual hydroxyapatite in its chromatographic properties. Granulated hydroxyapatite obtained has a high specific capacity and can be repeatedly used in experiments (up to 50 chromatographic cycles). Native high-polymeric T2 phage DNA was practically quantitatively eluated from the column. Conditions for chromatography of some proteins (lysozyme, RNase, DNase) are described. Fractionation and purification of T2 and T3 bacteriophages and TMV are carried out by means of chromatography on granulated hydroxyapatite.
D002845:Chromatography / Q000379:methods*; D003090:Coliphages / Q000032:analysis*; D003460:Crystallization; D004247:DNA / Q000032:analysis*; D006882:Hydroxyapatites / Q000138:chemical synthesis / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis*; D012313:RNA / Q000032:analysis*
D006882:Hydroxyapatites; D011506:Proteins; D012313:RNA; D004247:DNA
Khromatografiia nukleinovykh kislot, belkov i nekotorykh fagov na granulirovannom gidroksiapatite