Cholesterol, 7-ketocholesterol and 25-hydroxycholesterol uptake studies and effect on 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity in human fibroblasts.
In human fibroblasts two oxidized derivatives of cholesterol, 7-ketocholesterol and 25-hydroxycholesterol, but not cholesterol itself, are potent inhibitors of 3-hydroxy-3-methylglutaryl co-enzyme A reductase (mevalonate: NADP+ oxidoreductase (Co-enzyme A acylating), (EC 1.1.1.34), the rate-limiting enzyme in sterol biosynthesis. In addition, these derivatives of cholesterol are effective regulators in cells from homozygous familial hypercholesterolemic individuals. The differences in the inhibitory potencies of the sterols cannot be explained in terms of the amount of uptake into the cell.
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors*; D001692:Biological Transport; D002478:Cells, Cultured; D002784:Cholesterol / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D003470:Culture Media; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006888:Hydroxycholesterols / Q000378:metabolism; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007231:Infant, Newborn; D007664:Ketosteroids / Q000378:metabolism; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood; D008297:Male; D013816:Thermodynamics; D013997:Time Factors
D003470:Culture Media; D006888:Hydroxycholesterols; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007664:Ketosteroids; D008074:Lipoproteins; D002784:Cholesterol; D000429:Alcohol Oxidoreductases