Improved method for the simultaneous determination of phenobarbital, primidone and diphenylhydantoin in patient's serum by gas-liquid chromatography.
A quantitative gas-liquid chromatographic method for the simultaneous determination of phenobarbital, primidone and diphenylhydantoin in human serum is described. A double extraction is employed to improve the removal of interfering substances. The N,N-dimethyl derivatives of the compounds are prepared by "oncolumn" methylation with 50% Methelute. Decomposition of phenobarbital to N-methyl-a-phenylbutyramide was negligible if the contact time with Methelute was less than 10 min. The drugs were stable in serum for at least two weeks. This procedure provides a rapid, sensitive, selective and accurate method for the routine determination of serum concentrations of three of the most commonly prescribed anticonvulsant drugs.
D000927:Anticonvulsants / Q000592:standards; D002849:Chromatography, Gas; D004355:Drug Stability; D006801:Humans; D008722:Methods; D008745:Methylation; D010634:Phenobarbital / Q000097:blood*; D010672:Phenytoin / Q000097:blood*; D011324:Primidone / Q000097:blood*
D000927:Anticonvulsants; D011324:Primidone; D010672:Phenytoin; D010634:Phenobarbital