[A method of rational particle counting (author's transl)].
A method is described, which simplifies the counting of particles in a known volume. The counting of neurons of a brain region is used as an example for this procedure. The total volume is processed by histological serial sections. The total area of all sections of this volume is divided into counting squares by an ocular grid, from which we get a sample. This sample is described by the frequency distribution. The real number of particles in the volume is estimated by the values of the frequency distribution. The way of dividing, the size and the way of selection of these counting squares for a certain exactness of the estimation is determined. The method can be used for non-systematic distribution of particles in morphological structures.
D000818:Animals; D001921:Brain / Q000166:cytology*; D002452:Cell Count / Q000379:methods*; D002466:Cell Nucleolus; D002467:Cell Nucleus; D008297:Male; D008853:Microscopy; D008867:Microtomy; D008954:Models, Biological; D009474:Neurons / Q000166:cytology*; D011336:Probability; D012788:Shrews / Q000033:anatomy & histology*
Eine Methode zur rationellen Partikelzählung