1-beta-D-arabinofuranosylcytosine-induced malignant transformation of hamster and rat cells in culture.
The potent antileukemic chemotherapeutic drug, 1-beta-D-arabinofuranosylcytosine (ara-C), was observed to transform malignantly secondary hamster fetal cells as well as established rat cells in vitro. Results indicated that: (a) hamster cells altered by treatment with ara-C are morphologically indistinguishable from cells transformed with benzo(a)pyrene; (b) the ara-C-induced transformation can be observed under conditions of little or no inhibition of DNA synthesis and little or no cytotoxicity; (c) the transformation can occur with only a 6-hr exposure to ara-C; and (d) the transformation of hamster cells seems to require cellular DNA synthesis for cells in S phase are much more sensitive to ara-C-induced transformation than are G1-arrested cells. Representative transformed colonies of hamster and rat cells produced rapidly growing fibrosarcomas in inoculated newborn hamsters and rats, respectively. Control cells remained nontumorigenic.
D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D006224:Cricetinae; D003561:Cytarabine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D005333:Fetus; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology; D051381:Rats; D014184:Transplantation, Homologous
D001580:Benzopyrenes; D003561:Cytarabine; D004247:DNA