Amino acid transport in placental slices. Mechanism of increased accumulation by prolonged incubation.
The accumulation of alpha-aminoisobutyric acid by placental slices is increased dramatically upon prior incubation of the slices in amino acid-free, buffered saline. This increase is inhibited by inhibitors of protein synthesis and is accompanied by an increased V for the transport process. While alternative explanations are discussed, these data suggest that the incubation effect may be mediated through an increase in the number of available transport sites which are synthesized during the incubation period. Incubation with an amino acid mixture diminishes the increase as well as general protein synthesis, suggesting that a reduced availability of amino acids may initiate compensatory changes in the synthesis of cellular transport proteins.
D000596:Amino Acids / Q000494:pharmacology; D000621:Aminoisobutyric Acids / Q000378:metabolism*; D001692:Biological Transport; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D010920:Placenta / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D011691:Puromycin / Q000494:pharmacology
D000596:Amino Acids; D000621:Aminoisobutyric Acids; D011691:Puromycin; D003513:Cycloheximide