Genetic variation of in vitro cytolytic activity and in vivo rejection potential of non-immunized semi-syngeneic mice against a mouse lymphoma line.
Spleens of normal young mice of certain genotypes contain lymphocytes that can kill strain A-derived YAC-1 and some other in vitro-grown Moloney lymphoma lines in a 51Cr-release cytotoxic test. We have previously shown that mouse strains can be classified as high or low reactors in this test. F(1) hybrids between low- and high-reactive strains are high-reactive. In the present study, strain-A mice and eight different A F(1) hybrids were tested in parallel for their spleen-cell-mediated killing effect in vitro and their ability to reject graded numbers of YAC ascites or in vitro cultivated cells in vivo. There was a clear correlation between in vitro cytotoxicity and in vivo rejection in all tested genotypes. In segregating (A times C57Bl) times A backcross mice, in vivo rejection of YAC cells was H-2 linked. This is in line with the earlier backcross analysis of the in vitro cytotoxicity, suggesting a polygenic control with at least one H-2 linked factor.
D000818:Animals; D002460:Cell Line; D003433:Crosses, Genetic; D003601:Cytotoxicity Tests, Immunologic; D008040:Genetic Linkage; D014644:Genetic Variation; D005838:Genotype; D006084:Graft Rejection; D006648:Histocompatibility; D006824:Hybridization, Genetic; D008214:Lymphocytes / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D014184:Transplantation, Homologous