Sialoglycopeptides isolated from human arteriosclerotic tissue.
Sclerotic and non-sclerotic tissues of intima-media of human aorta were digested with pronase, after preliminary extraction of saline (1%)-soluble substances and fat. Subsequently, sialoglycopeptide fractions were obtained from the pronase digests by fractionation with CPC (cetylpyridinium chloride). Complete separation of sialoglycopeptides was achieved by chromatography on a DEAE-Sephadex A-25 column at pH 7.2, followed by repeated chromatography on the same Sephadex column at pH 5.2. The purified sialoglycopeptides thus obtained showed heterogeneity of their carbohydrate chains, although they contained the same monosaccharides (galactose, mannose, glucose, glucosamine, galactosamine, fucose, and sialic acid). Heterogeneity was also observed in their peptide chains. The contents of hydroxyamino acids and alkali-sensitive linkages of these preparations indicated that the fractions eluted from the DEAE-Sephadex A-25 column at higher molarity of the eluting salt at pH 5.2 contained many more O-glycosidic linkages between hydroxyamino acis(s) and sugar(s) than those eluted at lower molarity. The present data indicate that the concentration of sialoglycoproteins in intimamedia of human aorta increases significantly in arteriosclerosis in terms of defatted dry tissue. Moreover, the chemical nature of the sialoglycoproteins was modified slightly in the disease.
D000368:Aged; D000596:Amino Acids / Q000032:analysis; D001011:Aorta / Q000032:analysis; D001161:Arteriosclerosis; D002850:Chromatography, Gel; D006020:Glycopeptides / Q000032:analysis / Q000302:isolation & purification*; D006027:Glycosides / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006801:Humans; D008875:Middle Aged; D012794:Sialic Acids / Q000032:analysis / Q000302:isolation & purification; D013055:Spectrophotometry, Infrared
D000596:Amino Acids; D006020:Glycopeptides; D006027:Glycosides; D006595:Hexosamines; D006601:Hexoses; D012794:Sialic Acids