Demonstration of ricin-binding sites on the outer face of azurophil and specific granules of rabbit polymorphonuclear leukocytes.
The presence of carbohydrate residues on the outer surface of PMN granules has been demonstrated by the use of ricin-conjugated ferritin. The binding of the lectin was inhibited by alpha-lactose. No difference in the binding densities of azurophil or specific granules was observed.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002241:Carbohydrates; D002458:Cell Fractionation; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000648:ultrastructure; D005293:Ferritins; D007785:Lactose / Q000378:metabolism; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D009504:Neutrophils / Q000648:ultrastructure*; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits; D012276:Ricin / Q000378:metabolism*
D002241:Carbohydrates; D010940:Plant Proteins; D005293:Ferritins; D012276:Ricin; D007785:Lactose