High-pressure liquid chromatographic analysis of penicillin G potassium and its degradation products.
An anion-exchange high-pressure liquid chromatographic system capable of separating penicillin G potassium from five of its degradation products was developed. The retention times were: penicillin G potassium, 17.5 min; DL-penicillamine, 4.5 min; benzylpenilloic acid, 7.0 and 8.0 min; benzylpenamaldic acid, 13.0 min; benzylpenicilloic acid, 19.5 min; and benzylpenillic acid, 22.0 min. In addition, the system permits quantification using linear calibration curves.
D001593:Benzyl Compounds / Q000032:analysis; D002852:Chromatography, Ion Exchange; D013058:Mass Spectrometry; D008722:Methods; D010396:Penicillamine / Q000032:analysis; D010400:Penicillin G / Q000031:analogs & derivatives / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D001593:Benzyl Compounds; D010396:Penicillamine; D010400:Penicillin G