Electron microscopic demonstration of cell surface carbohydrates by means of peroxidase and ferritin complexes of the Lens culinaris lection.
The use of Lens culinaris lectin for electron microscopic detection of D-mannose,- D-glucose and N-acetyl-D-glucosamine like sites on tumor cells, erythrocytes, erythrocyte ghosts, cultured rat liver cells and various tissues of mice is demonstrated. In addition to Lens culinaris lectin-peroxidase reaction (LeL-po reaction) the preparation of active Lens culinaris lectin-ferritin conjugate are described and the specificity of cytochemical reactions are demonstrated. Furthermore experiments by immuno freeze-etching are reported for topological analysis of the lectin receptors.
D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D001666:Binding Sites, Antibody; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis* / Q000648:ultrastructure; D005293:Ferritins / Q000276:immunology*; D005613:Freeze Etching; D005947:Glucose / Q000032:analysis; D006801:Humans; D037102:Lectins; D008358:Mannose / Q000032:analysis; D008722:Methods; D051379:Mice; D008854:Microscopy, Electron; D010544:Peroxidases / Q000276:immunology*; D051381:Rats
D002241:Carbohydrates; D037102:Lectins; D005293:Ferritins; D010544:Peroxidases; D005947:Glucose; D008358:Mannose; D000117:Acetylglucosamine