Growth dynamics of Agrobacterium tumefaciens in chemostat cultures limited by carbon source and mineral nutrients.
Agrobacterium tumefaciens was grown in a chemostat in a chemically-defined medium which hs alpha-methyl D-glucoside, magnesium, manganese, phosphate or urea as the growth-limiting nutrient. Steady-state biomass concentrations were dependent on the specific growth rate of the organism when alpha-methyl D-glucoside, manganese or phosphate were growth-limiting nutrients. During magnesium-limited growth, large undamped oscillations in biomass concentration occurred. In all chemostat cultures a variant organism was selected which had lost the ability to grow in the medium supplied, bur survived on products of carbon metabolism derived from the wild-type.
D003470:Culture Media; D014644:Genetic Variation; D008274:Magnesium; D008345:Manganese; D008757:Methylglucosides; D010710:Phosphates; D012231:Rhizobium / Q000254:growth & development* / Q000378:metabolism; D014508:Urea
D003470:Culture Media; D008757:Methylglucosides; D010710:Phosphates; D008345:Manganese; D014508:Urea; D008274:Magnesium