GLC determination of 17 alpha-ethynylestriol 3-cyclopentyl ether.
A rapid, sensitive, and accurate GLC method of analysis of a new estrogenic drug, 17 alpha-ethynylestriol 3-cyclopentyl ether, was developed. The drug and the internal standard, tetratriacontane, are dissolved in chloroform, and an aliquot is heated with N-trimethylsilylimidazole at 80 degrees for 30 min. The silylated sample is chromatographed using a column packed with 1% methyl vinyl silicone gum on Gas Chrom Q. Quantitation is achieved by computer calculation of the peak area ratios. The observed peak is the 16alpha,17beta-bistrimethylsilyl derivative of the new drug substance. The GLC method was applied to the quantitative determination of the estrogenic compound in a tablet formulation containing 25 mug/tablet.
D002849:Chromatography, Gas; D003201:Computers; D045166:Estradiol Congeners / Q000032:analysis*; D004964:Estriol / Q000031:analogs & derivatives; D013058:Mass Spectrometry; D008722:Methods; D009651:Norpregnatrienes / Q000032:analysis*; D011800:Quinestrol / Q000031:analogs & derivatives / Q000032:analysis*; D013607:Tablets / Q000032:analysis
D045166:Estradiol Congeners; D009651:Norpregnatrienes; D013607:Tablets; C010722:nylestriol; D004964:Estriol; D011800:Quinestrol