Automated immunochemical method for determination of urinary protein of plasma origin.
An automated continuous-flow procedure has been developed for the rapid determination of urinary proteins of plasma origin. Antiserum to whole human plasma was used as the reagent, and the antigen-antibody reactions were quantitated by nephelometry. By adding polyethylene glycol (mol wt 6000-7500) to the reaction medium, reaction time was decreased to less than 3 min; no sample blanks were required; and samples were analyzed at a rate of 70 per hour. Recovery studies yielded an average of 98.5% of the added protein. In-run replicate precision (CV) of the method was 1.45%; day-to-day precision was 2.58%.
D001322:Autoanalysis / Q000379:methods; D001798:Blood Proteins / Q000652:urine*; D006801:Humans; D007674:Kidney Diseases / Q000652:urine; D010949:Plasma / Q000276:immunology; D011092:Polyethylene Glycols
D001798:Blood Proteins; D011092:Polyethylene Glycols