The effect of 5-bromodeoxyuridine on the synthesis of nuclear and cytoplasmic DNA-binding proteins isolated from sea urchin embryos.
Newly synthesized DNA-binding proteins were isolated from the nuclei and, separately from, the cytoplasm of sea urchin mofula stage embryos. The presence of 5-bromodeoxyuridine during embryogenesis did not appear to alter the synthesis of either class of DNA-binding proteins. This result tends to argue that cell differentiation in early embryos is not regulated by differential synthesis of DNA-binding proteins. Sea urchin mofulae synthesize a broad range, by molecular weight, or cytoplasmic DNA-binding proteins which dissociate from sea urchin DNA-cellulose at relatively high salt concentrations (0.6-2.0 M NaCl). The most prominant of these apparently high-binding-affinity proteins has an approximate molecular weight of 33,000.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002454:Cell Differentiation; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism*; D002846:Chromatography, Affinity; D003593:Cytoplasm / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian; D008970:Molecular Weight; D011485:Protein Binding; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D012617:Sea Urchins; D014158:Transcription, Genetic
D011506:Proteins; D004247:DNA; D001973:Bromodeoxyuridine