Identification of bisphosphatidic acid and its plasmalogen analogues in the phospholipids of a marine bacterium.
A relatively nonpolar unidentified phospholipid (phospholipid X) , isolated from the gram-negative marine bacterium MB 45, was characterized both chromatographically and by chemical analysis. Phospholipid X was shown to be an acidic phospholipid without vicinal hydroxyl, free-amino, or amide groups. The presence of O-alkenyl groups was indicated by a positive reaction for plasmalogen. Mild alkaline methanolysis of phospholipid X yielded only glycerophosphoryglycerol as the derivative. Acetolysis produced only diacyl-glycerol monoacetate. Clevage of O-alkenyl chains by methanolic hydrochloride resulted in the formation of three lyso derivatives. It was estimated that 18.2% of phospholipid X was plasmalogen. From these data, together with chromatographic comparisons with standards, infrared spectra, a molecular weight estimation, and the determination of the glycerol-phosphate-acyl ester ratio, it was concluded that phospholipid X was bisphosphatidic acid mixed with its plasmalogen analogues.
D001419:Bacteria / Q000032:analysis*; D002854:Chromatography, Paper; D008970:Molecular Weight; D010712:Phosphatidic Acids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D010955:Plasmalogens / Q000032:analysis*; D012623:Seawater; D013057:Spectrum Analysis; D014871:Water Microbiology
D010712:Phosphatidic Acids; D010743:Phospholipids; D010955:Plasmalogens