Polypeptide composition of spleen necrosis virus, a reticuloendotheliosis virus.
The polypeptide composition of virions of spleen necrosis virus, a reticuloendotheliosis virus, was determined using electrophoresis on sodium dodecyl sulfate-containing, 10 percent polyacrylamide gels. Ten polypeptides were resolved. Four of these were present in minor and somewhat variable amounts. Two proteins, gp71 and gp22, contained D-glucosamine and were located on the outer surface of the lipid envelope, as demonstrated by lactoperoxidase-catalyzed iodination and by bromelain digestion. The results suggest that two of the minor proteins, p36 and p26, were also located on the outer surface, although they lacked D-glucosamine. Treatment of the virus with 0.25 percent Nonidet P-40 and 1 percent dithiothreitol produced a subparticle with a buoyant density of approximately 1.31 g/cm-3. This particle was relatively enriched with polypeptides p77, p62, and p50 and contained small amounts of three other polypeptides.
D004591:Electrophoresis, Polyacrylamide Gel; D008206:Lymphatic Diseases / Q000382:microbiology*; D010455:Peptides / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*; D014777:Virus Diseases / Q000382:microbiology*; D014780:Viruses / Q000032:analysis / Q000201:enzymology / Q000378:metabolism*
D010455:Peptides; D014764:Viral Proteins