The size and detergent binding of membrane proteins.
Sucrose density gradient centrifugation has been used to measure the binding of Triton X-100 above its critical micellar concentration to a variety of purified membrane and non-membrane proteins.  In addition, binding studies were done on the three proteins below the critical micellar concentration of detergent to distinguish between the interaction of proteins with detergent monomers and detergent micelles.  A procedure is described for the calculation of the molecular weight of these Triton X-100 protein complexes and measurements were made for opsin, plasma low density lipoprotein, the (Na-+ plus K-+)-dependent adenosine triphosphatase, the human red blood cell major sialoglycoprotein (PAS-1) and the human red blood cell minor glycoprotein (bandIII).  These proteins behave as monomers or dimers in detergent and bind between 0.28 and 1.12 g of detergent per g of protein.  A general method is also present for calculating the molecular size and shape of impure membrane proteins in detergent.  Finally, Triton X-100 was shown to replace bound Na dodecyl-SO4 on the minor glycoprotein of the red blood cell.
D000418:Albumins; D000818:Animals; D001001:Anura; D003386:Brachyura; D002417:Cattle; D002645:Chickens; D004285:Dogs; D004798:Enzymes; D004912:Erythrocytes; D006023:Glycoproteins; D006454:Hemoglobins; D006736:Horses; D006801:Humans; D008074:Lipoproteins; D008566:Membranes / Q000187:drug effects*; D008928:Mitochondria; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011485:Protein Binding; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D012168:Retinal Pigments; D012519:Sarcoplasmic Reticulum / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D012995:Solubility; D013552:Swine; D014907:Whales
D000418:Albumins; D004798:Enzymes; D006023:Glycoproteins; D006454:Hemoglobins; D008074:Lipoproteins; D000644:Quaternary Ammonium Compounds; D012168:Retinal Pigments; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols