[Application of the theory of oxygen transfer: determination of the Michaelis constant of glucose oxidase with respect to oxygen].
The oxidation of beta-D-glucose with glucose oxidase generally requires oxygen, which, under normal conditions is present at low concentrations in the reaction medium. Experiments show that glucose oxidase is no longer saturated by oxygen at enzyme concentrations greater than 0.4 mg.ml1. This is due to the decrease in the oxygen concentration of the solution. The value of the oxygen mass transfer coefficients and dissolved oxygen concentrations are determined. These dissolved oxygen concentrations are found to correlate with direct measurements with an oxygen electrode. From this, the Michaelis constant of glucose oxidase for oxygen is calculated. These experiments also show that oxygen is a limiting factor for this reaction.
D001234:Aspergillus niger / Q000201:enzymology; D001665:Binding Sites; D004305:Dose-Response Relationship, Drug; D004566:Electrodes; D005949:Glucose Oxidase / Q000378:metabolism*; D007700:Kinetics; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D013696:Temperature; D014867:Water / Q000032:analysis
D014867:Water; D005949:Glucose Oxidase; D010100:Oxygen
Application de la théorie du transfert d'oxygène: détermination de la constante de Michaelis de la glucose oxydase par rapport à l'oxygène