Purification and some characteristics of the coagulation factor IX from human plasma.
Non-activated coagulation factor IX was purified approx. 10,000-fold from human plasma. The final product was electrophoretically homogeneous and comprised a tingle polypeptide chain with a molecular weight of about 70,000 and a pI of 4.3-4.45. The N-terminal amino acid was glycine. The amino acid and the carbohydrate contents were analysed and a monospecific antiserum to the factor was raised in rabbits.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D005164:Factor IX / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D005998:Glycine / Q000032:analysis; D006801:Humans; D007106:Immune Sera; D008970:Molecular Weight; D010443:Peptide Chain Termination, Translational; D010455:Peptides / Q000032:analysis; D011817:Rabbits / Q000276:immunology
D000596:Amino Acids; D002241:Carbohydrates; D007106:Immune Sera; D010455:Peptides; D005164:Factor IX; D005998:Glycine