Increased thymidine uptake by methylcholanthrene-treated C3H/10T1/2 cells.
The uptake of 3H-thymidine in post-confluent cultures of methylcholanthrene-transformed C3H/10T1/I mouse embryo cells was markedly higher than in their non-transformed counterparts. In a reconstruction experiment as few as 2% transformed cells could be detected by increased thymidine uptake. Measurements made at various times up to 110 days in multi-chambered plates revealed that after 25 days methylcholanthrene-treated cultures incorporated significantly more thymidine than the acetone-treated controls. This increased uptake correlated with the appearance of Type III transformed foci.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D008748:Methylcholanthrene / Q000494:pharmacology*; D051379:Mice; D008809:Mice, Inbred C3H; D013268:Stimulation, Chemical; D013936:Thymidine / Q000378:metabolism*; D013997:Time Factors
D008748:Methylcholanthrene; D013936:Thymidine