Localization of lectins in legume cotyledons.
High-resolution techniques for the localization of lectins are described. Concanavalin A (Con A) and phytohaemagglutinin (PHA) are localized using a fluorescent method with (FITC)-labelled immunoglobulins which bind to the lectins in sections of jack and red kidney bean cotyledons. Specificity is defined by the use of specific sugar inhibitors. Both Con A and PHA are found in cytoplasmic sites. Lectins with beta-glycoside specificity are detected with red-coloured artificial carbohydrate antigens. The beta-galactosyl and beta-glucosyl antigens bind specifically to clusters of spherical bodies in the intercellular spaces, to cell wall sites, and to the periphery of the cytoplasm associated with the cell membrane.
D002473:Cell Wall / Q000648:ultrastructure; D003208:Concanavalin A / Q000302:isolation & purification; D003593:Cytoplasm / Q000648:ultrastructure; D037102:Lectins / Q000302:isolation & purification*; D037121:Plant Lectins; D010944:Plants / Q000648:ultrastructure*; D013045:Species Specificity
D037102:Lectins; D037121:Plant Lectins; D003208:Concanavalin A