Purification of porcine enterokinase by affinity chromatography.
A method is described for the purification of porcine enterokinase by affinity chromatography with p-aminobenzamidine as the ligand. Purification was completed by immunoadsorption with antisera raised to components binding non-biologically to the gel. The final enterokinase preparation was 2.3 times more active than the most active preparation previously described.
D000327:Adsorption; D000818:Animals; D002846:Chromatography, Affinity; D010450:Endopeptidases / Q000302:isolation & purification*; D004765:Enteropeptidase / Q000302:isolation & purification*; D008024:Ligands; D013552:Swine
D008024:Ligands; D010450:Endopeptidases; D004765:Enteropeptidase