A simple method for making new transducing lines of coliphage lambda.
A variety of novel transducing lines of phage lambda can be obtained by induction of a mixed culture of abnormal lysogens. Such a culture is simply made by mass lysogenization of a host lacking the normal prophage attachment site.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003090:Coliphages / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media; D005838:Genotype; D008242:Lysogeny; D011995:Recombination, Genetic; D014161:Transduction, Genetic; D010948:Viral Plaque Assay
D003470:Culture Media