[Separation of ribonucleoprotein particles (Hn-RNP) from nuclei of L cells, in two classes, by isopycnic centrifugation in metrizamide gradients].
Separation of nuclear Hn-RNP particles into two density classes, following isopy cnic centrifugation in metrizamide gradients, is reported. One class (density 1.31 g/ml) is rapidly labelled, and contains polydisperse heterogeneous high molecular weight particles, easily diffusible from intact nuclei under certain conditions. The other class (density 1.18 g/ml) is of lower molecular weight, not diffusible, and needs apparently a longer time to be labelled and/or to accumulate inside the nuclei.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis* / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D007739:L Cells / Q000032:analysis*; D008793:Metrizamide; D051379:Mice; D009698:Nucleoproteins / Q000302:isolation & purification*; D012334:RNA, Neoplasm / Q000302:isolation & purification*
D009698:Nucleoproteins; D012334:RNA, Neoplasm; D008793:Metrizamide
Séparation des particules ribonucléoprotéiques (Hn-RNP) des noyaux de cellules L, en deux classes, par centrifugation isopycnique en gradients de métrizamide