Tryptophan and cystein residues of the acetylcholine receptors of Torpedo species. Relationship to binding of cholinergic ligands.
Several methods were used to analyze for tryptophan in the acetylcholine (ACh) receptors purified from the electric organs of the electric rays, Torpedo californica and Torpedo marmorata. The best value of tryptophan was 2.4 mol %. When excited at 290 nm, both receptors fluoresced with a maximum at 336, but there was no change in the fluorescence emission spectra upon binding of carbamylcholine, d-tubocurarine, ACh, or decamethonium. The free SH content of the Torpedo receptors varied in different preparations, and was highest in that purified from fresh T. californica using deaerated solutions and dialysis under nitrogen, and lowest in that prepared from the aged lyophilized membranes of T. marmorata. The maximum free SH content was 20 nmol/mg of protein or 0.22 mol %, equal to at most 18% of the total cysteic acid residues. Reaction of either 33% or of all the SH residues with p-chloromercuribenzoate reduced maximum ACh binding to the pure receptor prepared from fresh T. californica by only 23%.
D000109:Acetylcholine / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003545:Cysteine / Q000032:analysis* / Q000378:metabolism; D004557:Electric Organ / Q000378:metabolism*; D004593:Electrophorus; D005399:Fishes / Q000378:metabolism*; D011950:Receptors, Cholinergic; D013045:Species Specificity; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000378:metabolism; D014364:Tryptophan / Q000032:analysis* / Q000378:metabolism
D000596:Amino Acids; D011950:Receptors, Cholinergic; D013438:Sulfhydryl Compounds; D014364:Tryptophan; D003545:Cysteine; D000109:Acetylcholine