[Effect of actinomycin D on RNA synthesis in a stationary culture of Chinese hamster cells stimulated to proliferate].
The study of the effects of actinomycin D on stationary cultures of Chinese hamster cells and those stimulated by medium changing has revealed that RNA synthesis is more sensitive in the latter, the difference in the first place being attributed to the rate of labeled uridine incorporation into cell nucleoli. There is no significant difference in H3-actinomycin D uptake between stationary and stimulated cells, but the latter incorporate more labeled actinomycin D into their nuclei. A pronounced variation in sensitivity to actinomycin D is observed during the prereplicative period of stimulated cells. The first part of this period is more sensitive, which may be due to the necessity for stimulated cells to synthesize a great number of ribosomes to enter the mitotic cycle and to proceed through it.
D000818:Animals; D006224:Cricetinae; D003609:Dactinomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects*; D012313:RNA / Q000096:biosynthesis*; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D003609:Dactinomycin; D012313:RNA; D013936:Thymidine; D014529:Uridine
Vliianie aktinomitsina D na sintez RNK v statsionarnoĭ kul'ture kletok kitaĭskogo khomiachka, stimulirovannoĭ k proliferatsii