[A simplified method for the fluorometric determination of 5-hydroxy-indoleacetic acid in the human liquor cerebrospinalis (author's transl)].
The fluorometric analysis of 5-hydroxy-indoleacetic acid by condensation with o-phthaldialdehyde has been modified by substituting a HCl-H2SO4 mixture at room temperature for hot concentrated HCl. The sensitivity of the method was increased by a factor of 2.7.
D000447:Aldehydes; D005470:Fluorometry; D006801:Humans; D006851:Hydrochloric Acid; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid*; D007202:Indicators and Reagents; D008722:Methods; D010795:Phthalic Acids; D013464:Sulfuric Acids
D000447:Aldehydes; D007202:Indicators and Reagents; D010795:Phthalic Acids; D013464:Sulfuric Acids; D006897:Hydroxyindoleacetic Acid; D006851:Hydrochloric Acid
Méthode simplifiée pour le dosage fluorométrique de l'acide 5-hydroxy-indoleacétique dans le liquide céphalo-rachidien humain