Antigen-induced lymphoblast transformation in the diagnosis of cow's milk allergic diseases in infancy and early childhood.
The in vitro lymphocyte stimulation of 17 milk-allergic and eight control children was carried out with beta-lactoglobulin, bovine serum albumin, bovine hair extract and phytohaemagglutinin. The blastic transformation was significant with at least one of the milk antigens in 15 of the patients, but only in one of the controls. In two clinical pictures, corresponding to milk allergy of Arthus type (Schönlein-Henoch purpura and Heiner pneumonitis), besides the high ratio of specific blastic transformation of the lymphocytes the cellular reaction to PHA was pathologically decreased. A significant correlation could be demonstrated between the degrees of blastic transformation to BSA and bovine hair extract.
D000818:Animals; D000941:Antigens; D001249:Asthma / Q000175:diagnosis; D001991:Bronchitis / Q000175:diagnosis; D004485:Eczema / Q000175:diagnosis; D005260:Female; D006197:Hair; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000175:diagnosis*; D007782:Lactoglobulins; D037102:Lectins; D008213:Lymphocyte Activation; D008297:Male; D008892:Milk; D012710:Serum Albumin, Bovine
D000941:Antigens; D007782:Lactoglobulins; D037102:Lectins; D012710:Serum Albumin, Bovine