Radioimmunoassay of estradiol-17beta in bovine peripheral plasma with and without chromatography.
An assay of estradiol-17beta (E217beta) in bovine peripheral plasma is described. The plasma is incubated with an antiserum to E217beta-BSA and the gamma-globulin fraction precipitated with ammonium sulphate. After extraction with diethyl ether E217beta in the precipitate is estimated by radioimmunoassay using a specific antiserum against E217beta-6-BSA. Plasma concentrations of E217beta during the normal estrous cycle determined by this method and by a method involving Sephadex LH-20 chromatography range from 4 to 23 pg/ml.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D003429:Cross Reactions; D004958:Estradiol / Q000097:blood*; D004971:Estrus; D005069:Evaluation Studies as Topic; D005260:Female; D008722:Methods; D008832:Microchemistry; D011247:Pregnancy; D011863:Radioimmunoassay; D012756:Sheep / Q000276:immunology; D013997:Time Factors; D014316:Tritium
D014316:Tritium; D004958:Estradiol