Chemical and metabolic properties of adenosine diphosphate ribose derivatives of nuclear proteins.
1. ADP-ribose is found in rat liver nuclei covalently bound to histone F1, to a non-histone protein, and to a small peptide. 2. A single unit of ADP-ribose, covalently bound to phosphoserine, was isolated from an enzymic hydrolysate of histone F1. ADP-ribose-bearing peptides were isolated from a tryptic digest of the histone. 3. It is proposed that the 1'-hydroxyl group of ADP-ribose is linked to the phosphate group of phosphoserine in histone F1. 4. The incorporation of 32P into ADP-ribose on histone F1 a parallels the DNA content through the cell cycle. An increased incorporation of the nucleotide into the other derivatives is observed during S phase. 5. It is suggested that the ADP-ribose derivative of histone F1 has a role in maintaining the G0 state and that one or both of the other derivatives is concerned with control of DNA synthesis.
D000247:Adenosine Diphosphate Sugars / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D006657:Histones / Q000378:metabolism; D008099:Liver / Q000032:analysis; D008297:Male; D009698:Nucleoproteins / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D011485:Protein Binding; D051381:Rats
D000247:Adenosine Diphosphate Sugars; D000596:Amino Acids; D006657:Histones; D009698:Nucleoproteins; D009702:Nucleoside Diphosphate Sugars; D010758:Phosphorus