Polysaccharides as labels for antibodies in electron microscopy.
Two polysaccharides, dextran 250 and dextran 70, were covalently linked to antibody molecules, antihuman immunoglobulin G and antihuman type O red blood cells. In electron microscope preparations exposed to lead citrate, polysaccharides, because they chelate lead, were quite dense. Polysaccharides served as a tag for the antibody molecules. Also, bacterial dextran 1355 was used to demonstrate antibody molecules on the surface of ascites tumor cells which are known to be producing a specific antibody to bacterial dextran 1355. The varying sized polysaccharide molecules that are readily available commercially, the high electron density of the polysaccharides after lead staining and a mild procedure for covalently linking polysaccharide to antibody make polysaccharides attractive as particulate labels for antibody in electronmicroscopy.
D000017:ABO Blood-Group System; D000818:Animals; D000906:Antibodies; D002460:Cell Line; D003911:Dextrans; D004912:Erythrocytes / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D007074:Immunoglobulin G; D008198:Lymph Nodes; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010954:Plasmacytoma; D011135:Polysaccharides, Bacterial; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology
D000017:ABO Blood-Group System; D000906:Antibodies; D003911:Dextrans; D007074:Immunoglobulin G; D011135:Polysaccharides, Bacterial