Studies on deoxyribonucleoproteins. IX. Subfractions of deoxyribonucleoproteins from rat and mouse liver.
By carrier-free continuous electrophoresis, deoxyribonucleoprotein from rat and mouse liver could be separated into two subfractions. The more anodic fraction (DNP I), comprising 5 - 8 per cent of the total, contains fewer proteins (two types of histones only). [3H]Cyclophosphamide caused in vivo a 2.5 times higher alkylation of the DNA in in DNP I than of the DNA in DNP II. These and additional results led to the suggestion of a structural model with DNP I as a spacer in the deoxyribonucleoprotein fiber.
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D003852:Deoxyribonucleoproteins / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006657:Histones / Q000302:isolation & purification; D008099:Liver / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009698:Nucleoproteins / Q000032:analysis*; D051381:Rats; D013045:Species Specificity
D003852:Deoxyribonucleoproteins; D006657:Histones; D009698:Nucleoproteins; D003520:Cyclophosphamide