Rat liver hexokinases during development.
The four glucose-phosphorylating isozymes from rat liver were separated by DEAE-cellulose column chromatography at several ages during development. The isozymes exhibited a sequential mode of appearance. The isozymes A, B, and C reached maximal values of activity at days +1, +3, and +7, respectively, decaying afterwards to the low characteristic adult value. Isozyme D activity was detected in very low levels as early as day +3, rising from day +18 to reach constant adult values at day +30. A fraction of the glucose phosphorylating activity was found associated with particulate material in prenatal and newborn rats. From day +15 onwards, no particulate activity could be detected. The particulate activity was found to be composed of three low-Km isozymes as of the corresponding soluble fraction.
D000375:Aging; D000818:Animals; D005260:Female; D005333:Fetus; D006593:Hexokinase / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000201:enzymology* / Q000254:growth & development; D008297:Male; D011247:Pregnancy; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D006593:Hexokinase