Brain gangliosides: increased incorporation of (1- 3H) glucosamine during training.
The incorporation of (1-3H)glucosamine into biochemical fractions of brain was studied in mice trained in a conditioned avoidance jump-up task, in mice yoked to the trained animals, and in undisturbed mice in their home cages. The (3H)glucosamine was injected subcutaneously 15 min before 15 min of training, and the mice killed after a total pulse time of one hour. There was a consistent and significant increase of about 21 percent of the incorporation of the 3H into the brain ganglioside fraction when trained mice were compared with quiet mice. This increase was not observed in any of the other chemical fractions studied, including the total chloroform-methanol-soluble compounds, the non-ganglioside lipids, and the chloroform-methanol-insoluble compounds (radioactivity principally in glycoprotein). Yoked mice showed an intermediate level of incorporation, exhibiting only a 12 percent increase in the ganglioside fraction. When the individual ganglioside species were analyzed by thin-layer chromatography the changed incorporation was not specific for any ganglioside species.
D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism*; D007858:Learning / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012794:Sialic Acids / Q000378:metabolism; D013997:Time Factors
D005732:Gangliosides; D012794:Sialic Acids; D005944:Glucosamine