Hydrophobic chromatography of hepatitis B surface antigen on 1,9-diaminononane or 1,10-diaminodecane linked to agarose.
A series of omega-aminoalkyl-agarose differing in the number of carbon atoms in the alkyl chains was prepared and used for chromatography of hepatitis B surface antigen (HBSAg). HBSAg was separated from the major part of serum proteins by adsorption to columns of 1,9-diaminononane or 1,10-diaminodecane linked to agarose beads (Sepharose 4B or 2B) followed by elution with 4 M-NaSCN.
D000473:Alkanes; D002499:Centrifugation, Density Gradient; D002847:Chromatography, Agarose / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D003959:Diamines; D006511:Hepatitis B Antigens / Q000302:isolation & purification*; D013861:Thiocyanates; D014462:Ultrafiltration
D000473:Alkanes; D003959:Diamines; D006511:Hepatitis B Antigens; D013861:Thiocyanates