The early effects of ouabain on potassium metabolism and rate of proliferation of mouse lymphoblasts.
Murine lymphoblasts grown in suspension culture in the presence of ouabain showed a dose dependent and sequential decrease in 86Rb+ (K+ analogue) influx, cellular potassium content, and growth rate. An increase in eosin staining and a decrease in cell number was observed after two hours in the presence of 1 mM ouabain; 1 muM ouabain was without effect on any of the parameters measured. Ouabain inhibition was rapidly and completely reversible at concentrations that were not cytotoxic.
D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D008223:Lymphoma / Q000378:metabolism*; D051379:Mice; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism*; D011868:Radioisotopes; D012413:Rubidium; D013054:Spectrophotometry, Atomic
D011868:Radioisotopes; D010042:Ouabain; D012413:Rubidium; D011188:Potassium