Application of open-tubular column/gas-liquid chromatography to the analysis of complex mixtures of branched-chain fatty acids.
Methyl and butyl esters of branched-chain fatty acids of subcutaneous triacylgycerols from barley-fed lambs were subjected to urea-adduction and the resulting fractions were analyzed as methyl esters by gas-liquid chromatography on wall-coated open-tubular columns. The chromatogram of each fraction was less complex than that of the original and there was evidence to suggest that, in the original mixture of branched-chain acids, components resistant to urea-adduction were present, but their occurrence was obscured in chromatograms by the presence of monomethyl-substituted acids.
D002849:Chromatography, Gas / Q000379:methods*; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D005990:Glycerol / Q000032:analysis; D008745:Methylation; D014508:Urea
D004952:Esters; D005227:Fatty Acids; D014508:Urea; D005990:Glycerol