[Protein makeup of rabbit myofibrils determined by a disc eletrophoretic method in the presence of sodium dodecyl sulfate].
The protein subunit composition of isolated myofibrils of rabbit skeletal muscle is studied by polyacrylamide gel disc-electrophoresis in the presense of sodium dodecyl sulfate (SDS). The method of disc-SDS-electrophoresis is described in detail. The electrophoretic patterns of SDS-solubilized myofibrils obtained by disc-SDS-electrophoresis and by SDS-electrophoresis in continuous buffer system according to Weber and Osborn are compared. The former results in a markedly improved resolution and allows to discover some additional protein components, the origin of these additional components being discussed. A standard curve is given for determination of polypeptide chain molecular weights by disc-SDS-electrophoresis.
D000818:Animals; D004589:Electrophoresis, Disc / Q000295:instrumentation / Q000379:methods*; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis*; D009210:Myofibrils / Q000032:analysis*; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate
D009124:Muscle Proteins; D012967:Sodium Dodecyl Sulfate
Belkovyĭ sostav miofibrill krolika, opredelennyĭ metodom disk-élektroforeza v prisutstvii dodetsilsul'fata natriia