A comparison of the chromatographic properties of various polyadenylate binding materials.
The suitability of various materials for messenger RNA isolation and possible fractionation was examined by comparing the chromatographic behaviour of synthetic poly(riboadenylic acid) [poly(A)] and ribosomal RNA on each suport. The ratios of poly(A) to ribosomal RNA capacities of the various materials under different chromatographic conditions were used to estimate the potential selectivity of the columns for messenger RNA isolation. Using this criterion, benzoylated cellulose and oligo(deoxythymidylate)-cellulose should be the most selective materials of those studied. The elution conditions (temperature or salt gradient) used to obtain bound poly(A) were investigated to determine the optimum conditions for possible messenger RNA fractionation. It is concluded that oligo(deoxythymidylate)-cellulose appears to be the most suitable support of those investigated for messenger RNA isolation and possible fractionation.
D001665:Binding Sites; D002482:Cellulose / Q000031:analogs & derivatives; D002846:Chromatography, Affinity; D009838:Oligodeoxyribonucleotides; D011061:Poly A / Q000032:analysis*; D012333:RNA, Messenger / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000032:analysis; D013696:Temperature; D013938:Thymidine Monophosphate
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A; D013938:Thymidine Monophosphate; D002482:Cellulose