Inhibition of RNA synthesis by acetyl salicylate and actinomycin D during early development in the mouse.
Experiments were designed to ascertain and compare the effects of acetyl salicylate and actinomycin D on RNA synthesis in mouse oocytes in vitro and in vivo. After exposure to the drugs the effects on RNA synthesis were measured by incorporation of [3H]uridine and autoradiography. The results indicate that acetyl salicylate inhibits RNA synthesis in the treated oocytes as does actinomycin D. The only difference in the effects of these two drugs is that salicylate inhibits RNA synthesis to a much lesser degree than does actinomycin D. Effects from a short exposure to salicylate may be reversible; the same effects with actinomycin D cannot be reversed. In utero exposure of the female fetus may lead to partial or total sterility (depending on the dose and time of exposure) of that fetus and/or abnormal development of the progeny from those mice (F2). These results suggest that RNA synthesis in early oogenesis is a vital part of later development of the oocytes in adult mouse ovary. Inhibition of RNA may be one of the causes of malformations and sterility.
D000818:Animals; D001241:Aspirin / Q000009:adverse effects / Q000494:pharmacology*; D001345:Autoradiography; D001947:Breeding; D003609:Dactinomycin / Q000009:adverse effects / Q000494:pharmacology*; D003864:Depression, Chemical; D005260:Female; D005313:Fetal Death / Q000139:chemically induced; D005865:Gestational Age; D007246:Infertility / Q000139:chemically induced; D008297:Male; D051379:Mice; D009865:Oocytes / Q000378:metabolism / Q000648:ultrastructure*; D010053:Ovary / Q000196:embryology; D010063:Ovum / Q000648:ultrastructure*; D010920:Placenta / Q000033:anatomy & histology; D011247:Pregnancy; D012313:RNA / Q000096:biosynthesis*; D012098:Reproduction / Q000187:drug effects; D014529:Uridine / Q000378:metabolism; D015053:Zygote
D003609:Dactinomycin; D012313:RNA; D001241:Aspirin; D014529:Uridine