The production of acidic polysaccharides by 5-bromodeoxyuridine-treated B16 mouse melanoma cells.
B16 melanoma cells were treated in culture with 5-bromo-2-deoxyuridine. The cell-associated and released proteoglycans and sialoglycopeptides were compared to those of control cultures treated with thymidine. The 5-bromo-2-deoxyuridine-treated cultures showed a marked reduction in the proportion of cell-associated proteoglycans and sialoglycopeptides, an increase in the synthesis of hyaluronic acid, the absence of high-molecular-weight chondroitin sulfate, and the presence of increased amounts of heparan sulfate in the media. In addition, the 5-bromo-2-deoxyuridine-treated cells had a higher DNA content and were larger than controls.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002478:Cells, Cultured; D002807:Chondroitin / Q000096:biosynthesis; D004247:DNA / Q000378:metabolism; D003864:Depression, Chemical; D005944:Glucosamine / Q000378:metabolism; D006020:Glycopeptides / Q000096:biosynthesis; D006497:Heparan Sulfate / Q000096:biosynthesis; D006820:Hyaluronic Acid / Q000096:biosynthesis; D008545:Melanoma / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis*; D011509:Proteoglycans / Q000096:biosynthesis; D012794:Sialic Acids; D013936:Thymidine / Q000494:pharmacology
D001973:Bromodeoxyuridine; D002807:Chondroitin; D004247:DNA; D005944:Glucosamine; D006020:Glycopeptides; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D011134:Polysaccharides; D011509:Proteoglycans; D012794:Sialic Acids; D013936:Thymidine