Separation and quantitative determination of impurities in tetracycline.
Anhydrotetracycline, epianhydrotetracycline, epitetracycline, and chlortetracycline are generally found as impurities in tetracycline. A new thin-layer partition chromatographic method is described in which the impurities are separated on a kieselguhr layer impregnated with ethylene glycol-water-acetone-ethyl acetate (2:2:15:15). Compared to previous methods, this determiniation is easier to perform and more versatile and it prevents rapid epimerization. The quantitative determination of the tetracyclines utilizes spectrophotometry or direct TLC fluorometry.
D002855:Chromatography, Thin Layer; D004340:Drug Contamination; D005470:Fluorometry; D008722:Methods; D013053:Spectrophotometry; D013752:Tetracycline / Q000032:analysis*
D013752:Tetracycline