Purification of a fibroblast growth factor from bovine pituitary.
The purification from bovine pituitary gland of a growth factor responsible for the control of animal cell division in tissue culture is reported. This growth factor is a polypeptide of 13,300 molecular weight and is homogeneous when analyzed by polyacrylamide gel electrophoresis, carboxymethyl-Sephadex gradient elution chromatography, and Sephadex G-50 chromatography. The yield of growth factor is 5 mg per kg of pituitary. It is active in stimulating DNA synthesis in 3T3 cells at concentrations as low as 2 times 10-13 M with saturation at 1 times 10-10 M.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D003619:Dansyl Compounds; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D010053:Ovary / Q000187:drug effects; D010902:Pituitary Gland / Q000032:analysis*
D000596:Amino Acids; D003619:Dansyl Compounds; D006133:Growth Substances; D005688:Galactosamine; D004247:DNA; D005944:Glucosamine