Analysis of programmed gene action by means of radiation chimaeras.
Agglutinability by H-2 antisera of neonatal erythrocytes appears early in some mouse strains (such as C57BL/10) and late in others (A, CBA, etc). This might be due to interstrain differences of the postulated temporal genes controlling in this case the developmental expression of H-2 gene products A similarr effect might be simulated by interstrain variation in neonatal size of erythrocytes (possibly reflecting their stage of maturation from the generally larger blasts) which could indirectly affect agglutinability through a different density of H-2 sites on the cell surface. Such correlation was, however, not found in this study. A possible involvement of an extracellular control mechanism was also ruled out by demonstrating that in allogeneic radiation chimaeras created reciprocally from late and early strain mice (used as irradiated recipients and donors of curative cell inocula) the appearance of donor-specific H-2 agglutinability conforms to the donor's timing type being unaffected by the contrasting host's environment. The delayed agglutinability in a late strain was further shown to concern not only neonatal erythrocytes, but also those recruited from stem cells in "adult" bone marrow. Finally, two strains (C67BL/10 and CBA) classified as, respectively, early and late by H-2 agglutinability were typed similarly on the basis of the time course of 59Fe-incorporation rate/mg. spleen in newly formed syngeneic radiation chimaeras. The question whether this coincidence is due to chance or to H-2 linkage of a common or rather separate control mechanisms is discussed.
D000371:Agglutination; D000818:Animals; D000917:Antibody Formation; D004912:Erythrocytes / Q000276:immunology; D005796:Genes; D006384:Hemagglutination; D006649:Histocompatibility Antigens; D006824:Hybridization, Genetic; D007504:Iron Radioisotopes; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D008815:Mice, Inbred Strains; D011828:Radiation Chimera; D013154:Spleen / Q000276:immunology
D006649:Histocompatibility Antigens; D007504:Iron Radioisotopes