The complexity of satellite deoxyribonucleic acid in a higher plant.
Purified satellite DNA from melon (Cucumis melo) was shown to contain at least two components from thermal-denaturation and renaturation studies. Two components were separated after partial renaturation, a fast-renaturing fraction similar in complexity to mouse satellite DNA, and one with 6000 times greater complexity. Both components renatured very accurately, indicating a minimum of sequence divergence. Centrifugation of the purified satellite DNA in Ag+/Cs2SO4 gradients resolved two major and several minor fractions. The two major fractions were only slightly enriched for fast- or slow-renaturing sequences.
D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000032:analysis; D004247:DNA; D004276:DNA, Satellite / Q000032:analysis; D004279:DNA, Viral; D006358:Hot Temperature; D007700:Kinetics; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D010944:Plants / Q000032:analysis*; D013045:Species Specificity
D004276:DNA, Satellite; D004279:DNA, Viral; D004247:DNA