[Proceedings: Iodine metabolism in isolated human thyroid cells (hyperplastic goitre and toxic adenoma) (author's transl)].
We present here data on hormone synthesis and thyroxine secretion by isolated human thyroid cells. Thyroid cells were dispersed by trypsinization. Thyroid tissue was surgically obtained from two euthyroid patients with hyperplastic goitre and from two thyrotoxic patients with toxic adenoma. In 6 hr incubation experiments, we observed that isolated human thyroid cells, i) concentrated iodide, ii) synthetized iodothyronines, iii) released thyroxine in the incubation medium. Each parameters was TSH dependant. That thyroxine release was due to a secretion process is suggested by similar and further documented data obtained with isolated hog thyroid cells. This technique may provide further help for thyroid physiopathology investigation in humans.
D000236:Adenoma / Q000378:metabolism; D000818:Animals; D006042:Goiter / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007455:Iodine / Q000378:metabolism; D013552:Swine; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D013963:Thyroid Hormones / Q000096:biosynthesis; D013964:Thyroid Neoplasms / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology; D013974:Thyroxine / Q000378:metabolism
D013963:Thyroid Hormones; D013972:Thyrotropin; D007455:Iodine; D013974:Thyroxine
Métabolisme de l'iode dans les cellules thyroïdienes humaines isolées. Goitres hyperplasiques et adénomes toxiques