Increased hepatic triacylglycerol secretion in fasted rats with ventromedial hypothalamic lesions.
Adult female rats with lesions in the ventromedial hypothalamic area and sham-operated controls were given Triton WR 1339 intravenously after 24 h without food for measurement of liver triacylglycerol secretion rate. Tritiated water was injected for measurement of lipogenesis in liver, perirenal and subcutaneous adipose tissues in vivo. The experiments were performed on unrestrained animals with a chronically implnted venous heart cannula after 24 h without food. By the use of this technique, anesthesia and handling of the animals during the experiments was avoided. The following differences in the lesioned animals compared to the sham-operated controls were found: relative hypertriglyceridemia. A significant increase of triacylglycerol accumulation in the plasma. Increased incorporation of 3H FROM 3H20 into liver fatty acids. The experiments demonstrate that hepatic lipid synthesis during fasting is greater in the lesioned than in the control animals, but not high enough to account for the increased triacylglycerol secretion. A shift in the hepatic metabolism of fatty acids, leading to greater triacylglycerol formation at the expense of other processes is therefore suggested. The possible role of insulin in these metabolic changes is discussed.
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D005215:Fasting; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D007031:Hypothalamus / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D014280:Triglycerides / Q000097:blood / Q000378:metabolism*; D014867:Water / Q000378:metabolism
D005227:Fatty Acids; D014280:Triglycerides; D014867:Water; D011092:Polyethylene Glycols