The presence of a high-molecular-weight (guanine-plus-cytosine)-rich segment at the 3' end of rabbit 28S ribosomal ribonucleic acid.
The 3' hydroxyl end of 28S L-rRNA (major RNA species of the larger subribosomal particle) was labelled by coupling its 2-hydroxy-3-naphthoic acid hydrazine with diazotized [3H]aniline. The RNA was hydrolysed partially with ribonuclease T1 and fractionated on Sephadex G-200. The results show that a highly structured segment with 78% G+C content and a number-average molecular weight of at least 1.0x10(5)-1.8x10(5) is located at the 3' hydroxyl end of the 28S rRNA molecule.
D000818:Animals; D003596:Cytosine / Q000032:analysis; D006147:Guanine / Q000032:analysis; D008970:Molecular Weight; D012335:RNA, Ribosomal / Q000032:analysis*; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis
D012335:RNA, Ribosomal; D006147:Guanine; D003596:Cytosine