Effect of triiodothyronine on chick embryo heart cells maintained in tissue culture.
The effects of L-triiodothyronine (T3) on heart cells were studied in chick embryo heart cells maintained in tissue culture. T3 at a concentration of 3 x 10(-7) M had no effect on the contraction rate of these cells, either acutely or after 24 hr. On the other hand, T3 in concentrations as low as 3 x 10(-9) M stimulated both 2-deoxy-D-(1-3H) glucose uptake and lactic acid production. The effect of T3 on both deoxyglucose uptake and lactic acid production was noticed only after a lag period. Lactic acid production was measured again after removal of T3 from the media, and was found to remain elevated for at least an additional five days.
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D002642:Chick Embryo; D003847:Deoxyglucose / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism*; D013997:Time Factors; D014284:Triiodothyronine / Q000494:pharmacology*
D007773:Lactates; D014284:Triiodothyronine; D003847:Deoxyglucose