The metabolic and phagocytic activities of leukocytes from patients receiving corticosteroid and radiation therapy, and patients with bacterial infections.
Peripheral blood leukocytes from patients given corticosteroid or radiation therapy, as well as patients with bacterial or viral infections, were studied with regard to the selected enzyme activities of the hexose monophosphate shunt (HMS). Glucose-6-phosphate dehydrogenase (G-6-PD), 6-phosphogluconate dehydrogenase (6-PGD) and reduced nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase were assayed spectrophotometrically on mixed leukocyte suspensions in isotonic glycerol. Enzyme activities of G-6PD and NADPH oxidase in patients receiving corticosteroid or radiation therapy were significantly lower than the enzyme activity of 6-PGD. In patients with bacterial infections, activities of the three enzymes increased but in patients with viral infections, only the activities of NADPH oxidase and G-6PD were slightly decreased. Nitroblue tetrazolium (NBT) dyereducing activities of neutrophils from patients receiving corticosteroid or radiation therapy were attenuated which coincides with the reduced activities of HMS enzymes. From these results, it is likely that the reduced activities of intraleukocytic HMS enzymes of patients receiving corticosteroid or radiation therapy are correlated with intracellular bactericidal activities which might result from the attenuated level of hydrogen peroxide production.
D000293:Adolescent; D000305:Adrenal Cortex Hormones / Q000009:adverse effects / Q000494:pharmacology* / Q000627:therapeutic use; D000328:Adult; D001424:Bacterial Infections / Q000201:enzymology*; D001932:Brain Neoplasms / Q000201:enzymology / Q000532:radiotherapy; D002648:Child; D002675:Child, Preschool; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D008180:Lupus Erythematosus, Systemic / Q000188:drug therapy; D008297:Male; D008875:Middle Aged; D009247:NADH, NADPH Oxidoreductases / Q000097:blood*; D009393:Nephritis / Q000188:drug therapy; D009580:Nitroblue Tetrazolium; D010587:Phagocytosis; D010734:Phosphogluconate Dehydrogenase / Q000097:blood*; D011878:Radiotherapy / Q000009:adverse effects; D012595:Scleroderma, Systemic / Q000188:drug therapy; D014594:Uterine Neoplasms / Q000532:radiotherapy; D014777:Virus Diseases / Q000201:enzymology*
D000305:Adrenal Cortex Hormones; D009580:Nitroblue Tetrazolium; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases