Development of a radioimmunoassay for etorphine (preliminary communication).
A radioimmunoassay for etorphine has been developed using antibodies raised in a rabbit against an etorphine-3-hemisuccinate-bovine serum albumin conjugate. Using tritiated etorphine as the label, the antiserum could be used at a final dilution of 1:4 and had an avidity of 1.8 times 10-9 L.M.-1. The sensitivity of the assay was 0.88 plus or minus 0.04 ng etorphine per ml of serum and there was no cross-reactivity with any of the six opiate alkaloids examined over the range 0-100 ng/ml.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002417:Cattle; D003429:Cross Reactions; D005048:Etorphine / Q000032:analysis* / Q000276:immunology; D007114:Immunization; D009019:Morphinans / Q000032:analysis*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine; D013386:Succinates / Q000032:analysis; D014316:Tritium
D000906:Antibodies; D009019:Morphinans; D013386:Succinates; D014316:Tritium; D012710:Serum Albumin, Bovine; D005048:Etorphine