Determination of sperm-antibodies in sera of sterile women by affinity-chromatography (affinity-chromatography of sperm-antibodies).
The total amount of humoral sperm antibody protein is determined following its isolation by affinity-chromatography on spermatozoa. Serum proteins are eluted by Sorensen phosphate buffer. Antibodies attached to spermatozoa suspended in Sephadex G 75 columns resist elution by Sorensen buffer. They can be quantitatively washed out by 8 ml Tris HCl-buffer pH 8,9 and be determined as proteins by the Folin-Ciocalteu method.
D000327:Adsorption; D000906:Antibodies / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007247:Infertility, Female / Q000276:immunology*; D008297:Male; D008722:Methods; D013094:Spermatozoa / Q000276:immunology*
D000906:Antibodies; D001798:Blood Proteins; D007136:Immunoglobulins