Isoelectric focusing of pure rat pancreatic juice in polyacrylamide gel : possibility of concomitant estimation of radioactivity incorporated into single identified protein constituents.
A method is described for the isoelectric focusing of rat pancreatic juice on columns of polyacrylamide gel containing urea. Twenty-six bands were separated, and 6 were identified. Application of this method of separation to in vivo radioactively labelled pancreatic juice resulted in bands which could be counted with precision. This procedure allowed also the expression of radioactivity of one constituent relative to any single band, or relative to total radioactivity, concomitantly.
D000178:Acrylamides; D000681:Amylases / Q000302:isolation & purification; D000818:Animals; D002250:Carbon Radioisotopes; D002268:Carboxypeptidases / Q000302:isolation & purification; D002918:Chymotrypsin / Q000302:isolation & purification; D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D007525:Isoelectric Focusing / Q000379:methods; D008049:Lipase / Q000302:isolation & purification; D010189:Pancreatic Juice / Q000201:enzymology*; D051381:Rats; D014357:Trypsin / Q000302:isolation & purification; D014508:Urea
D000178:Acrylamides; D002250:Carbon Radioisotopes; D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D014508:Urea; D008049:Lipase; D000681:Amylases; D002268:Carboxypeptidases; D002918:Chymotrypsin; D014357:Trypsin