Interaction of a non-histone chromatin protein (high-mobility group protein 2) with DNA.
1. The interaction with DNA of the calf thymus chromatin non-histone protein termed the high-mobility group protein 2 has been studied by sedimentation analysis in the ultracentrifuge and by measuring the binding of the 125I-labelled protein to DNA. The results have been compared with those obtained previously by us [Eur. J. Biochem. (1974) 47, 263-270] for the interaction of high-mobility group protein 1 with DNA. Although the binding parameters are similar for these two proteins, high-mobility group protein 2 differs from high-mobility group protein 1 in that the former appears to change the shape of the DNA to a more compact form. 2. The molecular weight of high-mobility group protein 2 has been determined by equilibrium sedimentation and a mean value of 26 000 was obtained. 3. A low level of nuclease activity detected in one preparation of high-mobility group protein 2 has been investigated.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002843:Chromatin / Q000032:analysis*; D003090:Coliphages / Q000032:analysis; D004247:DNA; D004279:DNA, Viral; D003851:Deoxyribonucleases / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007457:Iodine Radioisotopes; D007700:Kinetics; D046911:Macromolecular Substances; D008433:Mathematics; D008970:Molecular Weight; D009698:Nucleoproteins; D011485:Protein Binding; D013950:Thymus Gland / Q000032:analysis
D002843:Chromatin; D004279:DNA, Viral; D007457:Iodine Radioisotopes; D046911:Macromolecular Substances; D009698:Nucleoproteins; D004247:DNA; D003851:Deoxyribonucleases