The action of plasma amine oxidase on beta-haloamines. Evidence for proton abstraction in the oxidative reaction.
The action of plasma amine oxidase upon beta-Br-ethylamine beta-Cl-ethylamine, beta-OH-phenylethylamine, and beta-Cl-phenylethylamine was examined. Beta-Br-ethylamine is a substrate and irreversible inactivator of the enzyme. The enzyme becomes covalently labeled by the inactivator. Approximately 2 mol of inactivator are incorporated per mol of enzyme (MW 170,000). The reduced enzyme is not inactivated. The enzyme catalyzes the elimination of HCl from beta-Cl-phenylethylamine to produce phenylacetaldehyde. The rate of the elimination reaction is comparable to the normal oxidative reaction. We conclude that the occurrence of this elimination reaction establishes the ability of the enzyme to catalyze proton abstraction from C-1 of the substrate and that proton abstraction occurs during the catalytic oxidation normally catalyzed by plasma amine oxidase. Beta-Cl-ethylamine is only oxidized to corresponding aldehyde. Beta-OH-phenylethylamine is neither oxidized, nor does elimination occur. It is a competitive inhibitor in the oxidation of benzylamine and in the elimination of HCl from beta-Cl-phenylethylamine.
D000818:Animals; D001595:Benzylamine Oxidase / Q000097:blood; D001966:Bromine; D002417:Cattle; D002713:Chlorine; D005021:Ethylamines / Q000138:chemical synthesis; D007700:Kinetics; D008995:Monoamine Oxidase / Q000097:blood*; D010084:Oxidation-Reduction; D010627:Phenethylamines / Q000138:chemical synthesis; D013329:Structure-Activity Relationship
D005021:Ethylamines; D010627:Phenethylamines; D002713:Chlorine; D001595:Benzylamine Oxidase; D008995:Monoamine Oxidase; D001966:Bromine