Molecular properties of multiple forms of acid phosphatase from horse liver.
1. Horse liver acid phosphatase was separated into two partially purified fractions differing in molecular weight (enzyme I about 100 00, enzyme II about 25 000). 2. Enzyme I was separated into several subfractions by DEAE-cellulose chromatography and isoelectric focusing. 3. Molecular weight, sedimentation coefficient and effective molecular radii were determined for acid phosphatases I and II by gel filtration and density-gradient centrifugation.
D000135:Acid Phosphatase / Q000032:analysis* / Q000145:classification; D000818:Animals; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D006736:Horses / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight
D000135:Acid Phosphatase