A rapid modified gas chromatographic assay for esterase activity.
A rapid modified procedure for the gas chromatogrpahic determination of esterase activity was studied. Aliphatic esters such as ethyl n-butyrate, n-propul n-butyrate, n-butyl n-butyrate and n-amyl n-butyrate were used as substrates and acetone was chosen as the most suitable solvent for dissolving the substrates in order to avoid alcoholysis. The enzyme reaction was started in a mixture of 0.03 M phosphate buffer, pH 7.90, containing an adequals, an aliquot of the reaction solutionwas injected directly on to a gas chromatograph and the alcohols produced were separated.
D000818:Animals; D002087:Butyrates / Q000378:metabolism; D002849:Chromatography, Gas; D004950:Esterases / Q000097:blood / Q000378:metabolism*; D005260:Female; D005410:Flame Ionization; D006801:Humans; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D013552:Swine
D002087:Butyrates; D004950:Esterases