Evidence against the proposed interaction of thionitrobenzoate with protein disulphide bonds.
Thionitrobenzoate was prepared from 5,5'-dithiobis-(2-nitrobenzoic acid), and its reaction with several disulphide-containing proteins was examined under various conditions. No evidence for any cleavage of disulphide bonds was obtained. The claim by Robyt et al. [Arch. Biochem. Biophys. (1971) 147, 262-269] that thionitrobenzoate allows the quantitative determination of disulphide bonds in proteins is not substantiated.
D001665:Binding Sites; D004220:Disulfides; D004228:Dithionitrobenzoic Acid; D007328:Insulin; D009113:Muramidase; D009579:Nitrobenzoates; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins; D013438:Sulfhydryl Compounds; D014357:Trypsin
D004220:Disulfides; D007328:Insulin; D009579:Nitrobenzoates; D011506:Proteins; D013438:Sulfhydryl Compounds; C011136:thionitrobenzoic acid; D004228:Dithionitrobenzoic Acid; D009113:Muramidase; D014357:Trypsin