Effect of X-irradiation on vitamin B12 binding capacity to intrinsic factor.
The effects of whole-body X-irradiation on vitamin B12-protein complex formation in gastric juice after oral administration of [57Co]-B12 have been studied. Two proteins with B12-binding activity have been isolated by gel filtration from gastric juice. 57Co-activity, recovered from B12-protein complex in gastric juice, is found to be about 30% less in the X-irradiated rat. In serum, vitamin B12 is mainly associated with alpha1-globulin. Radioactivity distribution in serum globulins after intraperitoneal injection of [57Co]-B12 was similar in control and X-irradiated rats.
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D005750:Gastric Juice / Q000528:radiation effects*; D007437:Intrinsic Factor / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D011485:Protein Binding / Q000528:radiation effects*; D011830:Radiation Effects; D051381:Rats; D013997:Time Factors; D014805:Vitamin B 12 / Q000378:metabolism / Q000528:radiation effects*; D014965:X-Rays
D001798:Blood Proteins; D007437:Intrinsic Factor; D014805:Vitamin B 12