ATP metabolism in tn/tn mouse embryos.
Total ATP, ATP/ADP ratios, and the rates of synthesis and turnover of ATP were determined for t12-, tw32- and T+/T+-litters at the 2-cell, 4-cell, 8-cell, early morula, late morula and late blastocyst stages. The results show that tn-litters have excessive rates of ATP metabolism up to the cleavage stage immediately before the stage of lethality for most tw32/tw32 embryos (early morula) and most t12/t12 embryos (late morula); mutant ATP metabolism then falls below control levels. After the death of the tn homozygotes, ATP metabolism in the tn-litters (composed of only T+/T+ and T+/tn embryos) returns to control levels. These data are discussed in relation to the phenotypes of the homozygous tn embryos.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis / Q000378:metabolism*; D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D005260:Female; D005804:Genes, Lethal; D005808:Genes, Recessive; D006720:Homozygote; D008297:Male; D051379:Mice / Q000196:embryology*; D008815:Mice, Inbred Strains; D009154:Mutation
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate