[Isolation and physical chemical properties of rat hemopexin].
Rat hemopexin was purified by a procedure involving three different steps : ammonium sulfate precipitation, rivanol precipitation and DEAE-cellulose chromatography with concave gradient of molarity. Purity of the preparation was checked by three different methods : analytical ultracentrifugation, immunoelectrophoresis and acrylamide gel electrophoresis. The principal physical properties were studied. The amino acid and carbohydrate composition was determined and compared with that of human and rabbit hemopexin.
D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004975:Ethacridine; D006466:Hemopexin / Q000032:analysis* / Q000302:isolation & purification; D006601:Hexoses / Q000032:analysis; D006801:Humans; D007122:Immunoelectrophoresis; D008297:Male; D011817:Rabbits; D051381:Rats; D014461:Ultracentrifugation
D000596:Amino Acids; D000606:Amino Sugars; D006601:Hexoses; D006466:Hemopexin; D004975:Ethacridine
Isolement et propriétés physico-chimiques de l'hémopexine de rat