Chromium-51 release from human blood platelets by anti A antibody.
Platelets were obtained by differential centrifugation from Group A donors and were incubated with Na2-51Cr04. After washing to remove excess chromium, the platelets were exposed for one hour to Anti A antibody which showed agglutinating activity only. Chromium release was no greater than that of controls. Hemolytic Anti A in the presence of rabbit complement released 15 percent more chromium than controls. Anti P1A1, a platelet specific antibody, when incubated with platelets in the presence of rabbit complement released 45 percent more chromium than controls. It appears that Anti A antibody is not as destructive to platelets as a platelet specific antibody is, even when the Anti A antibody shows hemolytic activity with red cells. These observations are in agreement with previous in vivo studies of ABO incompatible platelets.
D000017:ABO Blood-Group System / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies; D001792:Blood Platelets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D001803:Blood Transfusion; D002498:Centrifugation; D002857:Chromium / Q000378:metabolism*; D002860:Chromium Radioisotopes; D003165:Complement System Proteins; D004912:Erythrocytes / Q000276:immunology; D006461:Hemolysis; D006801:Humans; D007518:Isoantibodies; D011817:Rabbits / Q000276:immunology
D000017:ABO Blood-Group System; D000906:Antibodies; D002860:Chromium Radioisotopes; D007518:Isoantibodies; D002857:Chromium; D003165:Complement System Proteins