Chromatin-associated RNA content of heterochromatin and euchromatin.
Chromatin from TLT hepatoma cells, mouse liver cells, and mouse brain cells was fractionated by differential centrifugation into a pellet, enriched with heterochromatin, and a supernatant, enriched with euchromatin. The pellet was found to contain more than twice as much of a particular species of chromatin-associated RNA per milligram chromatin DNA as did the supernatant. This chromatin-associated RNA was also found to be associated with the transcriptionally inactive chromatin of mature avian erythrocytes. Bull sperm, whose genome is known to be completely inactive, was used as the source in the preparation of sperm heads. Bull sperm head RNA appeared to consist of a single, low molecular weight species which migrated on polyacrylamide gels at a rate just slightly slower than the aforementioned chromatin-associated RNA. The results are interpreted as indicating that this chromatin-associated RNA is more prevalently associated with the heterochromatic fraction of chromatin. It is postulated that this chromatin-associated RNA might constitute a structural component of heterochromatin.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis; D006570:Heterochromatin / Q000032:analysis*; D008099:Liver / Q000032:analysis; D008297:Male; D051379:Mice; D009928:Organ Specificity; D012313:RNA / Q000032:analysis* / Q000097:blood; D012334:RNA, Neoplasm / Q000032:analysis*; D013045:Species Specificity; D013094:Spermatozoa / Q000032:analysis
D002843:Chromatin; D006570:Heterochromatin; D012334:RNA, Neoplasm; D012313:RNA