Affinity chromatography of human leukocyte and diploid cell interferons on sepharose-bound antibodies.
Interferons produced in human peripheral leukocytes (LE) and foreskin fibroblast (FS-4) cells were subjected to affinity chromatography on Sepharose-bound globulins from rabbits immunized with these interferons. Anti-LE interferon sera neutralized both interferons, but titers against FS-4 interferon were consistently lower than those against LE interferon. Anti-FS-4 interferon sera neutralized only FS-4 but not LE interferon. Accordingly, affinity columns constructed with anti-FS-4 globulin excluded LE but not FS-4 interferon, whereas those prepared with anti-LE interferon globulin bound and eluted both LE and FS-4 interferons. Purification of native interferons of both types on anti-LE interferon-Sepharose ranged from 680- to 3,600-fold and recoveries from 72 to 126%. Specific activities of eluate pools varied from 4 to 30 times 10-6 reference (B, 69/19) units per milligram protien.
D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D002846:Chromatography, Affinity; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D007114:Immunization; D007372:Interferons / Q000302:isolation & purification*; D007962:Leukocytes / Q000032:analysis; D008297:Male; D011817:Rabbits / Q000276:immunology; D012685:Sepharose
D000906:Antibodies; D007372:Interferons; D012685:Sepharose