The preparation of membranes of some human T-mycoplasmas and the analysis of their carbohydrate content.
Purified membranes were prepared from seven human T-mycoplasmas of which four are laboratory strains and three isolates from patients with nonspecific urethritis. The T-mycoplasmas were resistant to osmotic shock and sonication. Membranes were obtained only after lysing most of the T-mycoplasmas by four passes through a cell fractionator at 40,000 psi and separating the membranes from the unlysed cells by differential centrifugation. The membranes contained between 1 and 7% carbohydrates by dry weight. Mannose, galactose, and glucose were identified with glucose in the largest concentration.
D002241:Carbohydrates / Q000032:analysis*; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000032:analysis; D006801:Humans; D014509:Ureaplasma / Q000032:analysis* / Q000648:ultrastructure
D002241:Carbohydrates