Occurrence of a new cephalosporoate in a culture broth of a Cephalosporium acremonium mutant.
A new metabolite was isolated from the culture filtrate of a deacetylcephalosporin C-producing mutant, derived from Cephalosporium acremonium ATCC 14553, by means of adsorption on activated carbon, column chromatography on DEAE-Sephadex A-25 and gel filtration through a Sephadex G-10 column. The compound was identified as D-5-amino-5-carboxyvaleramido-(5-formyl-4-carboxy-2H, 3H, 6H-tetrahydro-1, 3-thiazinyl) glycine by spectral analyses, elucidation of hydrolysis products of the compound, and comparison of characteristics of the compound with those of a synthetic authentic compound.
D000164:Acremonium / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D002511:Cephalosporins / Q000096:biosynthesis* / Q000138:chemical synthesis / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D003470:Culture Media; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D000596:Amino Acids; D002511:Cephalosporins; D003470:Culture Media