Induction of 16alpha-hydroxylase in cultured human lymphocytes.
Lymphocytes grown for 72 hr in the presence of mitogenic agents exhibited a nine-fold increase in 16alpha-hydroxylase activity over a 24-hr period following the addition of 17beta-estradiol as an inducing agent. The major metabolite of 17beta-estradiol produced by induced lymphocytes showed a chromatographic mobility indistinguishable from that of estriol. Enzymatic activity increased linearly with cell numbers, was inhibited by CO, and exhibited no induced increase in the presence of cycloheximide. A population survey indicated about 68% of a randomly selected Caucasian group to be essentially uninducible for 16alpha-hydroxylase.
D001189:Aryl Hydrocarbon Hydroxylases; D002248:Carbon Monoxide / Q000494:pharmacology; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D065727:Cytochrome P-450 CYP2C8; D065729:Cytochrome P-450 CYP2C9; D004790:Enzyme Induction / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D039181:Steroid 16-alpha-Hydroxylase; D013250:Steroid Hydroxylases / Q000096:biosynthesis*
D004958:Estradiol; D002248:Carbon Monoxide; D003513:Cycloheximide; D013250:Steroid Hydroxylases; C450260:CYP2C9 protein, human; D065729:Cytochrome P-450 CYP2C9; D001189:Aryl Hydrocarbon Hydroxylases; C450259:CYP2C8 protein, human; D065727:Cytochrome P-450 CYP2C8; D039181:Steroid 16-alpha-Hydroxylase