Isolation and structure determination of a further metabolite of diftalone.
A further metabolite of diftalone, a new antiinflammatory drug, has been identified as 7,14-dihydroxyphthalazino [2,3-b] phthalazine-5,12(7H,14H)-dione, on the basis of physico-chemical properties. The free metabolite was isolated from guinea-pig urine, where it is present as such and as the beta-glucuronide. This structure confirms the selective susceptibility of the methylene group of diftalone towards biological hydroxylation.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000652:urine*; D001692:Biological Transport; D006168:Guinea Pigs; D006900:Hydroxylation; D009682:Magnetic Resonance Spectroscopy; D010793:Phthalazines / Q000032:analysis / Q000652:urine*; D011724:Pyridazines / Q000652:urine*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D000893:Anti-Inflammatory Agents; D010793:Phthalazines; D011724:Pyridazines