A simple nonradioisotope technic for the determination of platelet life-span.
Acetylsalicylic acid was shown both in vivo and in vitro to prevent the platelet lipid peroxidation normally induced by the aggregating agents thrombin and epinephrine, and the sulfhydryl inhibitor N-ethylmaleimide. After aspirin ingestion, there was a significant reduction (p smaller than 0.005) in platelet lipid peroxidation, with a gradual return to base-line values over a 10-day period. With these values, a normal platelet survival curve was constructed with a mean half-life of 4.4 days (range of 2.9 to 5.9 days). These values agree with the standard 51-Cr survivals in three patients with chronic idiopathic thrombocytopenic purpura. Half-lives of 1.0, 2.5, and 4.1 days by lipid peroxide technic compared with 1.9, 2.5, and 3.9 days by the concurrent use of 51-Cr. Thus, the technic may be used to measure platelet survival.
D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D002470:Cell Survival; D002860:Chromium Radioisotopes; D002908:Chronic Disease; D004837:Epinephrine / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D006207:Half-Life; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000097:blood; D008722:Methods; D010545:Peroxides / Q000097:blood; D010974:Platelet Aggregation / Q000187:drug effects; D011696:Purpura, Thrombocytopenic / Q000097:blood; D013917:Thrombin / Q000494:pharmacology; D013997:Time Factors
D002860:Chromium Radioisotopes; D008055:Lipids; D010545:Peroxides; D013917:Thrombin; D005033:Ethylmaleimide; D001241:Aspirin; D004837:Epinephrine