Autoradiographic demonstration of DNA replication in ultrathin sections of plastic-embedded tissues using an exogeneous DNA polymerase.
A DNA-dependent DNA polymerase isolated from regenerating rat liver can mediate the incorporation of tritiated nucleoside triphosphates into acid-insoluble polydeoxyribonucleotides using the DNA contained in ultrathin sections of glycol methacrylate-embedded crab testis as initiator-template. (Summary see p. 186).
D000818:Animals; D001345:Autoradiography; D003386:Brachyura; D004254:DNA Nucleotidyltransferases; D004261:DNA Replication; D008099:Liver / Q000201:enzymology; D008115:Liver Regeneration; D008297:Male; D013698:Templates, Genetic; D013737:Testis / Q000166:cytology
D004254:DNA Nucleotidyltransferases