Method for extraction of glycoprotein hormones from plasma for use in radioimmunoassays.
Concanavalin-A covalently linked to Sepharose was used to extract the glycoproteins from a volume of human plasma about 10 times that volume usually added to conventional radioimmunoassay systems. Using a chorionic gonadoptropin (hCG) radioimmunoassay, hCG inhibition curves generated in the presence of the extracts were nearly identical to those generated by the conventional method; therefore, the sensitivity of the radioimmunoassays for glycoprotein hormones.
D000818:Animals; D006063:Chorionic Gonadotropin / Q000097:blood; D003208:Concanavalin A; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006023:Glycoproteins / Q000097:blood*; D006728:Hormones / Q000097:blood*; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D012685:Sepharose; D013972:Thyrotropin / Q000097:blood
D006063:Chorionic Gonadotropin; D006023:Glycoproteins; D006728:Hormones; D003208:Concanavalin A; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D012685:Sepharose