Modification of histone binding in calf thymus chromatin by protamine.
When calf thymus chromatin is incubated with protamine, the protein binds to DNA, forming a chromatin-protamine complex. The binding reaches a saturating level at the weight ratio of protamine to DNA of approximately 0.5. Although the saturated binding of protamine to DNA does not cause major displacement of histones from calf thymus chromatin, examination of the dissociation profiles by salt in combination with urea of protamine-treated chromatin shows that the histone-DNA interactions are markedly altered by such binding. The dissociation of histones from the chromatin-protamine complex requires less NaCl but the same concentration of urea as that for untreated chromatin, suggesting that the electorstatic interactions between the histones and DNA are decreased as a result of protamine binding. When protamine concentration is increased beyond that required for saturated binding to DNA during in vitro exposure of calf thymus chromatin to protamine, lysine-rich histone is completely displaced.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002843:Chromatin / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000032:analysis; D006657:Histones / Q000378:metabolism*; D046911:Macromolecular Substances; D011479:Protamines / Q000494:pharmacology*; D011485:Protein Binding; D011955:Receptors, Drug; D013950:Thymus Gland / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*
D002843:Chromatin; D006657:Histones; D046911:Macromolecular Substances; D011479:Protamines; D011955:Receptors, Drug; D004247:DNA