[On the absorption and excretion of raubasin in rats with particular reference to a method for the direct determination of the alkaloid].
A sensitive method has been developed for the determination of raubasin in biological fluids. The procedure involves preliminary solvent extraction of raubasin and its metabolites from alkaline body fluids into chloroform, followed by thin-layer chromatography and in situ fluorimetric quantitation. This method permits the quantitative assay of raubasin down to 0.02 mug with a standard deviation of less than 5%. Drug recovery from plasma is greater than 85%. The method has been applied to the measurement of raubasin concentrations found in rat plasma and urine after a single, oral dose of the alkaloid. A biliary elimination of the unchanged drug and of some metabolites has been demonstrated.
D000284:Administration, Oral; D000818:Animals; D001659:Biliary Tract / Q000378:metabolism; D002855:Chromatography, Thin Layer; D008297:Male; D008722:Methods; D051381:Rats; D046948:Secologanin Tryptamine Alkaloids / Q000008:administration & dosage; D013050:Spectrometry, Fluorescence; D015016:Yohimbine / Q000378:metabolism*
D046948:Secologanin Tryptamine Alkaloids; D015016:Yohimbine
Untersuchungen zur Resorption und Ausscheidung des Raubasins in Ratten unter Verwendung einer direkten Nachweismethode des Alkaloids