Alpha-lactalbumin production in human mammary carcinoma.
Alpha-Lactalbumin was isolated from mature human milk and utilized as an immunogen in rabbits. A radioimmunoassay was developed that was capable of detecting nanogram quantities of the antigen. Alpha-Lactalbumin synthesis was detected in human breast cancer cells (MCF-7) cultivated as a continuous cell line in vitro. Other human carcinoma epithelial cell lines (throat and cervix) failed to react in this assay. The ability to synthesize alpha-lactalbumin by breast carcinoma cells appeared to be independent of the addition of prolactin to the culture medium.
D001943:Breast Neoplasms / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism*; D002460:Cell Line; D005260:Female; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007768:Lactalbumin / Q000096:biosynthesis*; D010610:Pharyngeal Neoplasms / Q000378:metabolism; D011388:Prolactin / Q000494:pharmacology; D011863:Radioimmunoassay
D011388:Prolactin; D007768:Lactalbumin