A simplified procedure for synthesis of di-[14C]acyl-labeled lecithins.
A simplified procedure for synthesis of 1,2-di-[1'-14C]oleoyl-, 1,2-di-[1'-14C]linoleoyl-, and 1,2-di-[1'-14C]eicosatrienoyl-sn-glycero-3-phosphorylcholine is described. The method involves acylation of the CdCl2 complex of glycerophosphorylcholine with a 14C-labeled fatty acid in the presence of trifluoracetic anhydride and pyridine. The 14C-labeled lecithin is isolated in pure form by preparative thin-layer chromatography and alumina column chromatography in an overall yield of 12-24%. No isomerization or peroxidation of the unsaturated acids was detected.
D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D007553:Isotope Labeling; D008722:Methods; D008832:Microchemistry; D010713:Phosphatidylcholines / Q000138:chemical synthesis*
D002250:Carbon Radioisotopes; D010713:Phosphatidylcholines