Spin label studies on rat liver plasma membrane: calcium effects on membrane fluidity.
Spin-labeled stearic acid probes were incorporated into rat liver plasma membrane purified by a zonal centrifugation procedure. Temperature effects on the order parameter calculated from the spectra indicated the possible presence of a membrane phase transition at 20 degrees, in agreement with earlier studies in other systems. Addition of Ca2+ (1.96 mM) at 37 degrees increased the order parameter by 6.0 +/- 0.7%, suggesting a Ca2+-mediated decrease in membrane fluidity.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure*; D051381:Rats; D013696:Temperature
D002118:Calcium