The use of microfilters as an alternative to centrifugation in radioimmunoassay.
A microfilter was designed, together with a mechanical filtration apparatus for the separation of "antibody-bound" and "free" antigen in radioimmunoassay. A comparison of the results obtained using both filtration and centrifugation was carried out and the optimum incubation times, reaction conditions and washing volume for use with the microfilter were studied. The use of microfilters in radioimmunoassay gave reproducible results and offered a convenient means of automating the separation procedure.
D002498:Centrifugation; D005374:Filtration / Q000295:instrumentation; D008832:Microchemistry; D010316:Particle Size; D011863:Radioimmunoassay / Q000379:methods*; D013972:Thyrotropin / Q000032:analysis*; D013997:Time Factors
D013972:Thyrotropin