Primary cell culture for biochemical studies of human keratinocytes. A method for production of very large numbers of cells without the necessity of subculturing techniques.
Starting with large numbers of small split-thickness explants of human skin, it is possible to grow ample numbers of keratinocytes in vitro as primary cell cultures to permit biochemical studies without the need of subculturing techniques.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D008722:Methods; D012867:Skin / Q000378:metabolism*; D014020:Tissue Extracts
D003470:Culture Media; D014020:Tissue Extracts; D004247:DNA