[Pyrogen testing in vitro using the Limulus test].
The applicability of the Limulus test for the pyrogen test was checked in comparison to the pyrogen test in rabbits.  In 6 out of 24 lots of raw materials and drugs pyrogens could be detected by means of the pyrogen test in rabbits. 2 of these 6 lots showed positive reaction in the Limulus test, there were no false positive results.  Testing 7 bacterial strains in modified quantity of germs the Limulus test turned out to be more sensitive than the pyrogen test in rabbits.  The application of this in vitro test as a complement to the pyrogen test in rabbits for a certain kind of problems is discussed.
D000818:Animals; D001681:Biological Assay; D001831:Body Temperature; D005260:Female; D066298:In Vitro Techniques; D007263:Infusions, Parenteral; D008297:Male; D008722:Methods; D011749:Pyrogens / Q000032:analysis*; D011817:Rabbits; D012996:Solutions; D013535:Suspensions
D011749:Pyrogens; D012996:Solutions; D013535:Suspensions
Ein Beitrag zur in-vitro-Pyrogenprüfung mittels des Limulus-Tests