The effect of serum and experimental variables on the transferrin and reticulocyte interaction.
The transfer of iron from transferrin to the developing erythrocyte is a research area of high interest and considerable controversy. We have found that the results of transferrin-reticulocyte incubation studies are quite sensitive to the experimental procedures that are utilized. Reticulocytosis has been induced in rabbits by phelbotomy and phenylhydrazine injections. While the latter gives a higher reticulocyte count, the cells appear to exhibit an altered transferrin-membrane interaction. Transferrin has been iodinated by published methods utilizing chloramine-T and molecular iodine. The iodotransferrin products exhibit the same iron donation ability, however, evidence was found that the chloramine-T treatment leads to a nonspecific binding of transferrin to the reticulocyte. The means of saturating transferrin with 59Fe is also of prime importance. Fe(NH4)2(SO4)2 and especially FeCl3 were found to yield nonspecifically bound iron when added to transferrin or serum. This artifact was reflected in an altered transferrin-reticulocyte interaction. Using what we believe to be optimal conditions, the effect of serum on the transferrin-reticulocyte system was re-examined. The results clearly indicated an enhancement of iron uptake by reticulocytes in the presence of serum, as well as an accelerated incorporation of iron by the cytoplasmic fraction.
D000818:Animals; D001059:Apoproteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003470:Culture Media; D007501:Iron / Q000097:blood; D007700:Kinetics; D011485:Protein Binding; D011817:Rabbits; D011955:Receptors, Drug; D012156:Reticulocytes / Q000378:metabolism*; D013997:Time Factors; D014168:Transferrin / Q000378:metabolism*
D001059:Apoproteins; D003470:Culture Media; D011955:Receptors, Drug; D014168:Transferrin; D007501:Iron