A new method for intratubular blockade in micropuncture experiments.
Criteria for a reliable blockade of tubular flow are generally discussed and experimental evidence is presented that solid paraffin meets all the necessary criteria in rat kidney tubules when an immobile and tight sealing intratubular blockade is requested. A special microinjection apparatus for the application of paraffin is described. Furthermore, several alternative methods and materials have been tested and have been found to be inferior to solid paraffin.
D000818:Animals; D005782:Gels; D007684:Kidney Tubules / Q000502:physiology*; D008845:Microinjections / Q000295:instrumentation; D009399:Nephrons / Q000502:physiology; D010232:Paraffin; D011677:Punctures / Q000379:methods*
D005782:Gels; D010232:Paraffin