The growth of African horse-sickness virus in embryonated hen eggs and the transmission of virus by Culicoides variipennis Coquillett (Diptera, Ceratopogonidae).
Seven-day-old embryonated hen eggs were infected with African Horse Sickness virus by the yolk sac and intravenous routes. Virus reached a high titre in the blood of infected embryos. Culicoides variipennis midges which took a blood meal from infected eggs became infected with virus, and after 7 days at 26 degrees - 27 degrees C transmitted African Horse Sickness virus to uninfected eggs. C. variipennis may therefore be considered a biological vector of African Horse Sickness virus in the laboratory.
D000355:African Horse Sickness / Q000635:transmission*; D000356:African Horse Sickness Virus / Q000254:growth & development* / Q000302:isolation & purification; D000821:Animal Feed; D000818:Animals; D001769:Blood / Q000382:microbiology; D001826:Body Fluids / Q000382:microbiology; D002519:Ceratopogonidae / Q000382:microbiology; D002642:Chick Embryo / Q000382:microbiology; D005321:Extraembryonic Membranes; D005260:Female; D006736:Horses; D007303:Insect Vectors; D051379:Mice; D012087:Reoviridae / Q000254:growth & development*; D014817:Vitelline Membrane / Q000382:microbiology