Kinetic properties of hexokinase in resected temporal lobes of patients with drug-resistant epilepsy.
Properties of the cerebral glycolytic enzyme, hexokinase, were studied in biopsy samples of human temporal lobe, obtained during lobectomy for drug-resistant epilepsy and compared "blind" with contol biopsy samples of human cerebral cortex. No significant changes in the total activity or subcellular distribution of the enzyme were observed but the Km value for glucose was altered. The 17 control samples gave a normal mean value for Km (glucose) of 0.05 mM and the 14 epileptic samples gave a significantly higher mean value of 0.09 mM. The drugs used in previous treatment of the epilepsies were "scored" with respect to type and dose; analysis of these in relation to the kinetic results eliminated the possibility that the increase in Km value was an artifact due to the drugs. The observed change in enzyme kinetic properties is discussed in terms of potential interactions of small molecules with the isoenzymes of cerebral hexokinase.
D000367:Age Factors; D003593:Cytoplasm / Q000201:enzymology; D004833:Epilepsy, Temporal Lobe / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006593:Hexokinase / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008297:Male; D010672:Phenytoin / Q000627:therapeutic use; D011292:Premedication; D011324:Primidone / Q000627:therapeutic use; D013702:Temporal Lobe / Q000201:enzymology* / Q000473:pathology
D011324:Primidone; D010672:Phenytoin; D006593:Hexokinase