The cytological effects of infusion of luminal polyethylene glycol on the rat small intestinal mucosa.
An isolated sac of rat upper small intestine was prepared. Its lower end was drained to the skin. Solutions of known volume and composition were infused into the sac and the intestinal mucosa was examined by light and electron microscopy. Infusion of isosmotic (16.8 percent w/v) polyethylene glycol (PEG) resulted in prompt and progressively severe damage to enterocytes at the top of the villus, while goblet cells and basal-granulated cells were apparently unaffected. Thus, after 6-72 hrs a cap of goblet cells and basal-granulated cells accumulated at the tip of the villus, while the enterocytes underwent vacuolation and disintegration. Infusion of an isosmotic mixture of 1 percent PEG and 5.1 percent glucose caused no damage near the site of infusion, but damaged the intestinal sac further distally...
D000818:Animals; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000648:ultrastructure; D007421:Intestine, Small / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D011092:Polyethylene Glycols / Q000633:toxicity*; D051381:Rats; D014867:Water
D014867:Water; D011092:Polyethylene Glycols