Guanidinium-CsCl density gradients for isopycnic analysis of nucleic acids.
The addition of guanidinium chloride to CsCl gradients lowers the apparent density of RNA, DNA, and hybrid polymers in such a way that all three can be banded, fractionated, and analyzed in one gradient with essentially no damage to their chemical integrity or to their biological activity.
D002499:Centrifugation, Density Gradient / Q000379:methods*; D002500:Centrifugation, Isopycnic / Q000379:methods*; D002586:Cesium; D002712:Chlorides; D004247:DNA / Q000032:analysis; D006146:Guanidines; D009691:Nucleic Acid Denaturation; D009696:Nucleic Acids / Q000032:analysis*; D012313:RNA / Q000032:analysis
D002712:Chlorides; D006146:Guanidines; D009696:Nucleic Acids; D002586:Cesium; D012313:RNA; D004247:DNA