Further characterization of bovine pancreatic lipase.
1. The amino acid composition of bovine pancreatic lipase is very similar to that of porcine lipase. 2. Bovine lipase possesses a residue of lysine at the N-terminal position and a half cystine or a cysteine at the C-terminal position. 3. Bovine lipase contains two free sulfhydryl groups of different reactivities to 5, 5'-dithiobis-(2-nitrobenzoic) acid. One of these groups is buried in the native conformation of the enzyme and is fully titrated in 1.5 M urea when reaction is performed in the presense of 1 mM EDTA.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001565:Benzoates; D002268:Carboxypeptidases; D002417:Cattle; D004220:Disulfides; D008049:Lipase / Q000032:analysis* / Q000378:metabolism; D009574:Nitro Compounds; D010179:Pancreas / Q000201:enzymology*; D012967:Sodium Dodecyl Sulfate; D013438:Sulfhydryl Compounds / Q000032:analysis; D013552:Swine; D014508:Urea
D000596:Amino Acids; D001565:Benzoates; D004220:Disulfides; D009574:Nitro Compounds; D013438:Sulfhydryl Compounds; D012967:Sodium Dodecyl Sulfate; D014508:Urea; D008049:Lipase; D002268:Carboxypeptidases