[Activity and properties of RNA-dependent RNA polymerase induced in cell cultures infected with Venezuelan equine encephalomyelitis virus].
The activity and properties of RNA-dependent RNA-polymerase in cells infected with Venezuelan equine encephalomyelitis virus were studied. The maximum activity of the enzyme was found 4-5 hours after infection. The effect of two synthetic drugs (rimantadine and diacetylaminocyclohexenon) with antiviral activity against some viruses on RNA-polymerase was studied. Rimantadine was shown to have no effect on the synthesis and activity of the enzyme. Diacetylaminocyclohexenon added into the growth medium was found to inhibit production of RNA-polymerase by 35-40% without reducing the activity of the enzyme in vitro.
D000085:Acetates / Q000494:pharmacology; D000255:Adenosine Triphosphate; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001952:Bridged-Ring Compounds / Q000494:pharmacology; D002642:Chick Embryo; D003514:Cyclohexylamines / Q000031:analogs & derivatives / Q000494:pharmacology; D003516:Cycloparaffins / Q000494:pharmacology; D003597:Cytosine Nucleotides; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis / Q000378:metabolism*; D003609:Dactinomycin; D004666:Encephalitis Virus, Venezuelan Equine / Q000187:drug effects / Q000254:growth & development*; D005021:Ethylamines / Q000494:pharmacology; D005347:Fibroblasts / Q000201:enzymology; D006150:Guanine Nucleotides; D008274:Magnesium; D012367:RNA, Viral / Q000096:biosynthesis; D014316:Tritium; D014500:Uracil Nucleotides; D014529:Uridine; D014779:Virus Replication / Q000187:drug effects
D000085:Acetates; D000998:Antiviral Agents; D001952:Bridged-Ring Compounds; D003514:Cyclohexylamines; D003516:Cycloparaffins; D003597:Cytosine Nucleotides; D005021:Ethylamines; D006150:Guanine Nucleotides; D012367:RNA, Viral; D014500:Uracil Nucleotides; D014316:Tritium; D003609:Dactinomycin; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium; D014529:Uridine
Izuchenie aktivnosti i nekotorykh svoĭstv RNK-zavisimoĭ RNK-polimerazy, indutirovannoĭ v kul'ture kletok, zarazhennykh virusom venesuél'skogo éntsefalomielita loshadeĭ