[Inhibiting effect and mechanism of action of 5'-deoxy-5'-fluorothymidine on vaccinia virus reproduction].
5'-deoxy-5'-fluorothymidine in concentrations 2.5 times 10-4 to 2 times 10-3 M was found capable of inhibiting vaccinia virus reproduction in tissue culture. Its inhibiting effect was associated with the primary capacity to inhibit biosynthesis of thymidylic nucleotides in the stage of TDP and TTP synthesis which appears to be the consequence of inhibition of thydidylate kinase activity. Under these conditions accumulation of TMP is observed. The data obtained permit to consider 5'-deoxy-5'-fluorothymidine to be an "early" inhibitor of vaccinia virus reporduction, and its mechanism of action to be a new type of directed interference with vaccinia virus reproduction.
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002474:Cell-Free System; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts; D013936:Thymidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013942:Thymine Nucleotides / Q000096:biosynthesis; D014316:Tritium; D014616:Vaccinia virus / Q000187:drug effects* / Q000254:growth & development; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D013942:Thymine Nucleotides; D014316:Tritium; D013936:Thymidine
Ingibiruiushchiĭ éffekt i mekhanizm deĭstviia 5'-dezoksi-5'-ftortimidina na reproduktsiiu virusa ospovaktsiny