The effect of glucose and lactose on beta-D-galactosidase activity and formation of sclerotia in Sclerotium rolfsii.
Addition of 0.5% (w/v) lactose to a glucose-mineral mdeium (SM) induced formation of sclerotia and beta-D-galactosidase (beta-D-galactoside galactohydrolase)(EC 3.2.1.23) synthesis in Sclerotium rolfsii types A and R; These effects as well as lactose uptake were inversely related to glucose concentration within the tested range of 0.5 to 2.5% (w/v).  Transfer of lactose-grown colonies to a glucose-supplemented medium nullified the inducible effect of lactose on formation of sclerotia, whereas transfer to water agar did not. It is concluded that glucose nullifies the effect of lactose on S. rolfsii by interfering with its active uptake.
D005696:Galactosidases / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism*; D003904:Mitosporic Fungi / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D009024:Morphogenesis; D013172:Spores, Fungal / Q000254:growth & development
D005696:Galactosidases; D005947:Glucose; D007785:Lactose