In vivo activation of C3 revealed by crossed immunoelectrophoresis as a parameter of immunological activity in disease.
A technique suitable for the demonstration of in vivo activation of the third component of complement (C3) is described, and the results of detailed methodological studies are presented. The technique is a useful tool for monitoring the activity of the humoral immune system in disease states. The serum levels of complement components reflect a balance between synthesis and catabolic rates, while the present method gives a more dynamic picture of the state of the complement system. The results of the application of the technique to different groups of patients are presented.
D000743:Anemia, Hemolytic / Q000276:immunology; D000818:Animals; D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology; D003176:Complement C3 / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism*; D004355:Drug Stability; D006801:Humans; D007122:Immunoelectrophoresis; D008722:Methods; D011817:Rabbits / Q000276:immunology; D013997:Time Factors
D003176:Complement C3; D003165:Complement System Proteins