Immune precipitation of immunoglobulin producing polysomes from mouse myeloma cells.
MPC-11 myeloma cells synthesizing IgG were labeled in vitro with 3H-uridine and the cell lysates, or purified polysomes treated with rabbit antiserum against the purified MPC-11 protein. The specificity of the immune precipitation was tested by rabbit anti-egg albumin and by precipitation of polysomes from "non-producer" myeloma and HeLa cells. The specific precipitation was 10 to 15% of the total cytoplasmic RNA for the IgG-producing clone of MPC-11 and about 2% for the non-producer clone or HeLa cells. Up to 20% of the total polysomal and ribosomal RNA were precipitated from the IgG producing clone. This value correlated with the IgG synthesis of these cells which is also about 20% of the total protein produced. The results suggest that myeloma cells produce a relatively large quantity of IgG due to the presence of a large amount of specific m-RNA molecules.
D000818:Animals; D000906:Antibodies; D002999:Clone Cells; D007074:Immunoglobulin G / Q000096:biosynthesis*; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D011132:Polyribosomes / Q000276:immunology*; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis
D000906:Antibodies; D007074:Immunoglobulin G; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D010047:Ovalbumin