Microtubular proteins and concanavalin A receptors.
The inherent topographical distribution of Con A binding sites (CABS) is disperse or random in all cell types studied using hemocyanin to mark CABS in surface replicas. In virally transformed cells, the addition of Con A leads to the formation of clusters (CABS). A role for microtubules is suggested in this process since colchicine treatment of transformed cells and Con A addition lead to the aggregation of Con A into a "cap". During phagocytosis CABS are selectively removed from the surface. This selective movement is abolished by drugs that disrupt microtubules. Binding of Con A or RCA to intact cells at 37 degrees C leads to the removal of their receptors from the surface, presumably by "micropinocytosis".
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D051379:Mice; D008854:Microscopy, Electron; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D010587:Phagocytosis / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D011955:Receptors, Drug; D013997:Time Factors
D011506:Proteins; D011955:Receptors, Drug; D003208:Concanavalin A; D003078:Colchicine