Ageing and dopamine uptake by subcellular fractions of the C57BL/6J male mouse brain.
The uptake of dopamine (DA) by synaptosomes from the hypothalamus and striatum was reduced in healthy ageing mice at DA concentrations below 10(-7) M. Kinectic analysis indicates that ageing increases the Km of high affinity DA uptake processes by about 35%, but does not alter alter Vmax. l-Tyrosine and serotonin uptake at low concentrations was unaltered by ageing in the striatum and hypothalamus; no changes of L-norepinephrine uptake were found in the hypothalamus. We conclude that ageing may selectively affect DA uptake processes by neuronal membranes.
D000375:Aging; D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D012701:Serotonin; D014443:Tyrosine; D004298:Dopamine; D009638:Norepinephrine