Fluoride inhibition of the initiation of protein synthesis in the reticulocyte lysate cell-free system.
KF (30 mM) strongly inhibits polypeptide chain initiation in the reticulocyte lysate cell-free system.. Chain elongation is partially inhibited but proceeds to a significant extent with little initiation of new chains. Polysome breakdown is incomplete after incubations as long as 20 min. Under these conditions deacylated tRNA-Met accumulates in a fraction sedimenting faster than 120 S and thus may be associated with ribosomes bound to mRNA. Incubation of the system with KF results in the accumulation of a complex which can initiate synthesis of polypeptide chains in the presence of aurintricarboxylate; KF thus inhibits a step in initiation after that inhibited by aurintricarboxylate. The accumulation of deacylated tRNA-Met is correlated with the accumulation of the aurintricarboxylate-resistant complex. Both phenomema are dependent on KF and both are inhibited by aurintricarboxylate in the same range of concentrations which inhibit initiation of protein synthesis.
D000818:Animals; D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D001798:Blood Proteins / Q000096:biosynthesis*; D002474:Cell-Free System; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D005459:Fluorides / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008715:Methionine; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects*; D011132:Polyribosomes / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*; D013997:Time Factors
D001798:Blood Proteins; D001312:Aurintricarboxylic Acid; D012343:RNA, Transfer; D008715:Methionine; D007930:Leucine; D005459:Fluorides