Comparison of results for quantitative determination of morphine by radioimmunoassay, enzyme immunoassay, and spectrofluorometry.
The quantitative results (accuracy and precision) for determination of opiates by radioimmunoassay (RIA), enzyme immunoassay (EMIT), and spectrofluorometry on split samples are compared. A variety of physiological samples were studied, including random urine from a methadone maintenance clinic and postmortem urine, blood, bile, brain, and lung tissue from heroin-induced or heroin-related deaths. The opiate concentrations detected by the two immunoassay methods were in good agreement with each other in the absence of interfering substances which are believed to react with the antimorphine antibodies. The immunoassay results were in agreement within the relative standard deviation with the fluorometry results in 55% of the urine samples and 80% of the blood samples. The immunological methods are superior to fluorometry for quantitation of morphine in urine samples due to quenching interferences in fluorometry from urine. They were comparable to fluorometry for quantitation of morphine in blood samples.
D000328:Adult; D004798:Enzymes; D005260:Female; D005554:Forensic Medicine; D003932:Heroin / Q000506:poisoning; D006801:Humans; D007118:Immunoassay; D008297:Male; D009020:Morphine / Q000032:analysis* / Q000506:poisoning; D011863:Radioimmunoassay; D013050:Spectrometry, Fluorescence
D004798:Enzymes; D003932:Heroin; D009020:Morphine