5-Oxoprolinase from rat kidney, II. Molecular weight determinations.
The molecular weight of 5-oxoprolinase from rat kidney was estimated by gel filtration on Sephadex G-200 and G-150 to be 460 000 +/- 30 000. A value of 230 000 +/- 10 000 was obtained by zonal sedimentation in a sucrose gradient. Polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate yielded a molecular weightof 115 000 +/- 6 000. It is concluded that 5-oxoprolinase consists of four subunits of 115 000 daltons each. The dissociation or aggregation behavior of the enzyme seems to be influenced neither by the presence of the substrates 5-oxo-L-proline and MgATP2theta nor by the presence of the stabilizing compounds glutathione mercaptoethanol or dithioerythritol.
D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D002501:Centrifugation, Zonal; D002850:Chromatography, Gel; D004355:Drug Stability; D004589:Electrophoresis, Disc; D007668:Kidney / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011761:Pyrrolidonecarboxylic Acid; D051381:Rats; D013438:Sulfhydryl Compounds
D046911:Macromolecular Substances; D013438:Sulfhydryl Compounds; D000581:Amidohydrolases; D011761:Pyrrolidonecarboxylic Acid