[2 methods of preparing solvent systems for the chromatographic separation of organic acids].
The solvent system of alcohol-acid-water gradually forms an equilibrated amount of ester which facilitates the separation of di- and tri-carboxylic acids on paper chromatograms. Using the system of n-butanol-formic acid-water (3:1:1) as an illustration, two methods of accelerating the system ageing are demonstrated: 1) butanol-acid mixture at a required ratio is kept for several hours before water is added and 2) butanol-acid mixture is passed through the KU-2 layer in the H-form. The effect identical to that achieved as a result of 5-day storage of the solvent system under usual conditions can be reached with the first scheme during 6 hours and with the second scheme during 20-30 min.
D000143:Acids / Q000302:isolation & purification*; D000156:Aconitic Acid / Q000302:isolation & purification; D000440:Butanols; D002854:Chromatography, Paper / Q000379:methods*; D002951:Citrates / Q000302:isolation & purification; D004987:Ethers; D005561:Formates; D008293:Malates / Q000302:isolation & purification; D012997:Solvents; D013644:Tartrates / Q000302:isolation & purification; D013997:Time Factors; D014867:Water
D000143:Acids; D000440:Butanols; D002951:Citrates; D004987:Ethers; D005561:Formates; D008293:Malates; D012997:Solvents; D013644:Tartrates; D014867:Water; D000156:Aconitic Acid
Dva sposoba prigotovleniia sistem rastvoriteleĭ dlia khromatograficheskogo razdeleniia organicheskikh kislot