[The metabolism of C14-labeled phenylalanine and tyrosine in malaria-infected Culex females (author's transl)].
1. Culex females are fed on C14-phenylalanine or C14-tyrosine in sugar solution. Autoradiographic studies on homogenated females 1 or 4 days after feeding, show that the labeled amino acids are metabolized on the first day and are not detectable on the fourth day. 2. After increase of the amino acid concentration by saturation of the sugar solution with the unlabeled amino acid, the labeled acid and its metabolites are visable over a longer period of time. 3. Phenylalanine is metabolized to tyrosine and at least four other substances. Radioactivity on the starting point of the chromatogram can be interpreted as incorporation of tyrosine into proteins. 4. After infection with Plasmodium cathemerium, and feeding of C14-phenylalanine C14-tyrosine is demonstable over a longer period.
D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002845:Chromatography; D003465:Culex / Q000378:metabolism*; D005260:Female; D008288:Malaria / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D014443:Tyrosine / Q000378:metabolism*
D002250:Carbon Radioisotopes; D014443:Tyrosine; D010649:Phenylalanine
Der Umbau von radioaktivem Phenylalanin und Tyrosin in Malaria-infizierten Culex-Weibchen