Metabolism of (4-14C)progesterone and (7alpha-3H)pregnenolone by human ovaries perfused in vitro.
Nine human ovaries were perfused in vitro with [4-14C]progesterone and in addition one ovary with [7-3H]pregnenolone. From the perfusate unchanged progesterone and five different metabolites were isolated: 20alpha-dihydroprogesterone, 17alpha-hydroxyprogesterone, 16alpha-hydroxyprogesterone, 5alpha-pregnane-3,20-dione and 4-androstene-3,17-dione. In the ovarian tissue saturated pregnane derivatives were the main metabolites. When [3H]pregnenolone and [14C]progesterone were perfused simultaneously a stimulation of delta4-5-isomerase and 3beta-dehydrogenase activity by HCG was demonstrated.
D004092:20-alpha-Dihydroprogesterone / Q000096:biosynthesis; D000328:Adult; D000735:Androstenedione / Q000096:biosynthesis; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D006801:Humans; D006908:Hydroxyprogesterones / Q000096:biosynthesis; D066298:In Vitro Techniques; D007535:Isomerases / Q000378:metabolism; D008875:Middle Aged; D010053:Ovary / Q000201:enzymology / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D011277:Pregnanediones / Q000096:biosynthesis; D011278:Pregnanes / Q000096:biosynthesis; D011284:Pregnenolone / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D013268:Stimulation, Chemical
D006063:Chorionic Gonadotropin; D006908:Hydroxyprogesterones; D011277:Pregnanediones; D011278:Pregnanes; D004092:20-alpha-Dihydroprogesterone; D000735:Androstenedione; D011374:Progesterone; D011284:Pregnenolone; D010088:Oxidoreductases; D007535:Isomerases