Isolation and in vitro translation of human placental lactogen messenger RNA from human term placenta.
A messenger activity for HPL was identified in normal human term placentas. The mRNA was translated in rabbit reticulocyte cell-free system. The HPL synthesized was quantified by a specific immunoprecipitation and further identified by electrophoresis on sodium dodecyl sulfate polyacrylamide gel. The HPL synthesized in the reticulocyte lysate exhibited a molecular weight between 20,000 and 22,000 daltons similar to the active hormone. The messenger RNA activity for HPL corresponded to a sedimentation coefficient of 11-12 S. Furthermore the messenger activity for HPL was preferentially associated with membrane bound polyribosomes than with free polyribosomes.
D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D005260:Female; D006801:Humans; D008970:Molecular Weight; D010920:Placenta / Q000378:metabolism*; D010928:Placental Lactogen / Q000096:biosynthesis* / Q000276:immunology; D011132:Polyribosomes / Q000032:analysis / Q000378:metabolism; D011233:Precipitin Tests; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000302:isolation & purification / Q000378:metabolism
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D010928:Placental Lactogen