The relationship between GSH, GSSG and non-GSH thiol in GSH-deficient erythrocytes from Finnish landrace and Tasmanian merino sheep.
1. Two automated colorimetric methods have been developed for assaying the GSH and total thiol in protein-free extracts of erythrocytes. They employ as chromogens 5,5'-dithiobis-(2-nitrobenzoate) (DTNB) and alloxan. 2. The concentrations of GSH, GSSG and total non-protein thiol have been estimated in high and low GSH erythrocytes from Finnish Landrace and Tasmanian Merino sheep. 3. In both breeds of sheep low GSH cells were found to have low concentrations of total non-protein thiol and GSSG as well as of GSH. 4. Nevertheless high and low GSH cells have similar values for the oxidation-reduction potential of the GSH : GSSG couple.
D000496:Alloxan; D000818:Animals; D001322:Autoanalysis; D003956:Dialysis / Q000379:methods; D004228:Dithionitrobenzoic Acid; D004912:Erythrocytes / Q000032:analysis*; D005978:Glutathione / Q000097:blood / Q000172:deficiency*; D010084:Oxidation-Reduction; D012756:Sheep / Q000097:blood*; D013045:Species Specificity; D013438:Sulfhydryl Compounds / Q000097:blood*
D013438:Sulfhydryl Compounds; D000496:Alloxan; D004228:Dithionitrobenzoic Acid; D005978:Glutathione