A soluble cadmium-binding component in rat and dog spleen.
The spleens of cadmium-treated Sprague-Dawley rats and mongrel dogs were examined for cytoplasmic soluble cadmium-binding moieties following the cessation of parenteral cadmium exposure. Cell homogenates prepared in 0.25M sucrose were centrifuged at 29,000 g and the supernatant fractionated on a fractionating column (Sephadex G-75). Ultraviolet absorbing peaks that showed characteristically high 250-nm to 280-nm absorbences were further analyzed for cadmium content by atomic absorption spectrophotometry. Cadmium-binding fractions isolated from the spleens of both species were characteristically similar to rat kidney metallothionein-like substances isolated and identified by the same procedure. Molecular weights for the canine and rat spleen cadmium-binding components were estimated at 14,783 +/- 343 and 13,916 +/- 490, respectively. These preliminary data indicate the production and persistence of soluble metallothionein-like substances in spleen that bind and retain cadmium ions long after cadmium exposure has ceased.
D000818:Animals; D002104:Cadmium / Q000008:administration & dosage / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004285:Dogs; D005260:Female; D008297:Male; D008667:Metalloproteins / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats; D012995:Solubility; D013154:Spleen / Q000166:cytology / Q000378:metabolism*
D008667:Metalloproteins; D002104:Cadmium; D008668:Metallothionein