Procollagen peptidase: its mode of action on the native substrate.
Procollagen peptidase was recovered from the medium of human and mouse fibroblast cultures by precipitation with ammonium sulfate. The test substrate for the in vitro enzymatic reaction was radioactively-labeled, disulfide-linked procollagen prepared from the medium of human fibroblast cultures. The enzymatic digests were analyzed by electrophoresis in polyacrylamide gets containing sodium dodecyl sulfate and urea. The human and mouse enzymes reacted with the substrate to generate the same intermediates and final products. Procollagen peptidase acts as an endopeptidase which cleaves each of the three procollagen chains in turn. The final products of the reaction are collagen and a three-chain, disulfide-linked fragment derived from the nonhelical aminoterminal residues of procollagen.
D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002474:Cell-Free System; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D005347:Fibroblasts; D006801:Humans; D051379:Mice; D008956:Models, Chemical; D008970:Molecular Weight; D011392:Proline / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D012867:Skin; D014316:Tritium; D014364:Tryptophan / Q000378:metabolism
D002250:Carbon Radioisotopes; D011498:Protein Precursors; D014316:Tritium; D014364:Tryptophan; D003094:Collagen; D011392:Proline; D010450:Endopeptidases