Isolation and polymerization of brain actin.
The studies presented here confirm earlier reports that an actin-like protein is abundant in brain. However, when the traditional procedures for isolating muscle actin are applied to brain, many different proteins are extracted. Tubulin, a major protein in brain with properties similar to actin, is the major constituent. A method is described for isolating the "brain actin" to a purity of 90-95%. The isolation method begins with an extraction of bovine brain in low ionic strength buffer with ATP and sucrose. The extract is treated with NH4SO4, MgCl, and KCl and incubated  at 37 degrees C. A precipitate is formed which contains primarily tubulin and brain actin. Resolubilization of the brain actin is achieved with a low ionic strength buffer solution with sucrose and ATP. Further purification is accomplished by a cycle of polymerization-depolymerization. This "brain actin" shares with muscle actin the following properties: (1) Similar molecular weight and molecular charge as determined by SDS polyacrylamide gel and ordinary disc electrophoresis; (2) Polymerization to a filamentous form under the same conditions; (3) Contains 3-methylhistidine; (4) Vinblastine sulfate will induce filament formation.
D000199:Actins / Q000302:isolation & purification*; D000818:Animals; D001923:Brain Chemistry; D002021:Buffers; D002118:Calcium; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D003078:Colchicine; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D008274:Magnesium; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D011108:Polymers; D014404:Tubulin; D014747:Vinblastine
D000199:Actins; D002021:Buffers; D009419:Nerve Tissue Proteins; D011108:Polymers; D014404:Tubulin; D014747:Vinblastine; D008274:Magnesium; D003078:Colchicine; D002118:Calcium