Dose and time dependent luteotropic and luteolytic action of prostaglandin F2alpha in the luteinized rabbit ovary.
The mechanism of stimulatory and inhibitory action of PGF2alpha on ovarian steroidogenesis both under in vitro and in vivo conditions has been studied in the pseudopregnant rabbits. Short term incubation of the ovaries with PGF2alpha (2.82 times 10(-5)M) resulted in an increased synthesis of progesterone and 20alpha-OH P. The addition of PGF2alpha in the medium and further incubation of the ovaries obtained from rabbits that had been constantly infused with PGF2alpha (0.5 mug/min.) for two hours resulted in increased synthesis of these progestins. The ratio of progesterone to 20alpha -OH P was also enhanced under these conditions and thus supported the luteotropic action of small doses of PGF2 under short term incubations. However, as the amount of PGF2alpha infused was increased to 5 mug/min., the addition of PGF2alpha under in vitro conditions strikingly decreased the production of these progestins. The ratio decreased the production of these progestins. The ratio of progesterone to 20alpha -OH P was also decreased and thus was indicative of luteolytic action of higher doses of PGF2alpha. High doses of PGF2alpha (5.64 times 10(-4)M) failed to cause any significant change in the progestin synthesis under short term incubation. These results thus suggest that the luteotropic and luteolytic action of PGF2alpha in the luteinized rabbit ovary is dose and time dependent.
D006907:17-alpha-Hydroxypregnenolone / Q000096:biosynthesis*; D000085:Acetates / Q000378:metabolism; D000818:Animals; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D005260:Female; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D010053:Ovary / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000096:biosynthesis* / Q000378:metabolism; D011460:Prostaglandins F / Q000008:administration & dosage / Q000494:pharmacology*; D011555:Pseudopregnancy; D011817:Rabbits; D013268:Stimulation, Chemical; D013997:Time Factors
D000085:Acetates; D011460:Prostaglandins F; D006907:17-alpha-Hydroxypregnenolone; D011374:Progesterone