Inhibition of DNA chain growth by alpha-2'-deoxythioguanosine.
Mecca lymphosarcoma cells were incubated with (35-S)-alpha-2'-deoxythioguanosine for 8 hr and DNA was analyzed in alkaline sucrose gradients. 35-S radioactivity was found exclusively in a low-molecular-weight fraction. Pulse-chase experiments showed that 35-S-containing DNA fragments formed during the pulse were not incorporated into high-molecular-weight DNA following the chase. These results, together with the previous observation that (35-S)-alpha-2'-deoxythioguanosine was found predominantly in the terminal nucleoside position of DNA chains, suggested that alpha-2'deoxythioguanosine, once incorporated, terminates chain elongation.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000302:isolation & purification; D003853:Deoxyribonucleosides / Q000494:pharmacology*; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism*; D051379:Mice; D008806:Mice, Inbred AKR; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000378:metabolism; D013866:Thioguanine / Q000031:analogs & derivatives / Q000494:pharmacology
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D003853:Deoxyribonucleosides; D004247:DNA; D013866:Thioguanine