Molecular basis of Tn-polyagglutinability.
Spectrophotometric and gas-liquid chromatographic analyses on the carbohydrate moiety of tryptic erythrocyte glycopeptides from persons with Tn-syndrome reveal a selective lowering of the galactose and sialic acid content, the degree being dependent on the percentage of polyagglutinable cells. Alkaline borohydride specifically releases N-acetylgalactosaminitol, and the amount is correlated to the percentage of pathological acetylgalactosaminitol, and the amount is correlated to the percentage of pathological erythrocytes. It is concluded that the alkali-labile carbohydrate chains of Tn-polyagglutinable red cells solely consist of N-acetylgalactosamine linked to serine or threonine. Experiments with heterophile agglutinins whose specificity is known are in line with the above-mentioned results. As judged from SDS-polyacrylamide gel electrophoresis the three major membrane glycoproteins are affected to a different extent by the defect.
D000743:Anemia, Hemolytic / Q000276:immunology*; D000910:Antibodies, Heterophile / Q000032:analysis; D001786:Blood Glucose / Q000032:analysis; D001797:Blood Protein Electrophoresis; D002462:Cell Membrane / Q000276:immunology; D002849:Chromatography, Gas; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000032:analysis; D006020:Glycopeptides / Q000032:analysis; D006384:Hemagglutination; D006386:Hemagglutination Tests; D006601:Hexoses / Q000032:analysis; D006801:Humans; D007970:Leukopenia / Q000276:immunology*; D008970:Molecular Weight; D012794:Sialic Acids / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D013577:Syndrome; D013921:Thrombocytopenia / Q000276:immunology*; D014357:Trypsin
D000910:Antibodies, Heterophile; D001786:Blood Glucose; D006020:Glycopeptides; D006601:Hexoses; D012794:Sialic Acids; D012967:Sodium Dodecyl Sulfate; D014357:Trypsin