Radioimmunoassay for cortisol using antibodies against prednisolone conjugated at the 3-position.
A radioimmunoassay for the measurement of cortisol in plasma was developed. The procedure requires a one-step precipitation of the proteins in plasma or a one-step extraction with methylene chloride in urine. The method is precise, accurate, sensitive, and very specific. The primary antibody was prepared against prednisolone-3- carboxymethoxime-bovine serum albumin. The method is sensitive to 12.5 pg/ml of plasma. The antiserum has little interference from other endogenous steroids and metabolites of cortisol. 0830 hour plasma levels of cortisol in twenty normal human adults averaged 150 +/- 33 ng/ml.
D000328:Adult; D006801:Humans; D006854:Hydrocortisone / Q000097:blood* / Q000276:immunology; D011239:Prednisolone / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods*
D011239:Prednisolone; D006854:Hydrocortisone