Tumor-associated and embryonic antigens in soluble fractions of a chemically-induced rat colon carcinoma.
Soluble intracytoplasmic protein fractions and solubilized tumor membrane preparations produced by 3 M KC1 or papain treatment were isolated from a 1,2 dimethylhydrazine-HC1 (DMH)-induced rat colon carcinoma, DHM-BU 1. Solubilized preparations were fractionated by DEAE cellulose and Sephadex G200 column Chromatography. Crude extracts and fractionated materials were tested for their ability to inhibit the cytotoxicyt of lymph-node cells (LNC) from rats bearing isografts of an N-methyl-N-nitro-N-nirosoguanidine (NG)-induced colon carcinoma, ng-W1...
D000230:Adenocarcinoma / Q000139:chemically induced / Q000276:immunology*; D000818:Animals; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D004127:Dimethylhydrazines; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008769:Methylnitronitrosoguanidine; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D051381:Rats; D011915:Rats, Inbred BUF; D011920:Rats, Inbred WF; D014184:Transplantation, Homologous
D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D004127:Dimethylhydrazines; D008769:Methylnitronitrosoguanidine