Direct determination of polyamines in human serum by radioimmunoassay.
Antipolyamine antibodies were produced following immunization of New Zealand white rabbits with thyroglobulin spermine conjugate, using multiple-site injection and macrophage-harvesting techniques. The antispermine antibody in a radioimmunoassay system cross-reacted with spermidine (22%), putrescine (1%), diaminopropane (6%), and cadaverine (0.16%). No reaction with L-lysine, ornithine, or histamine was noted. Using this antibody, the radioimmunoassay for direct determination of serum polyamines was developed. A sensitivity of 200 pg was achieved on human serum samples. This radioimmunoassay would appear to be a useful tool for the detection and monitoring of a variety of pathophysiological states associated with abnormal polyamine metabolism, i.e., cancer.
D000818:Animals; D002103:Cadaverine; D003429:Cross Reactions; D006736:Horses / Q000276:immunology; D006801:Humans; D009369:Neoplasms / Q000097:blood; D011073:Polyamines / Q000097:blood* / Q000276:immunology; D011700:Putrescine / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013095:Spermidine / Q000276:immunology; D013096:Spermine / Q000276:immunology
D011073:Polyamines; D013096:Spermine; D002103:Cadaverine; D013095:Spermidine; D011700:Putrescine