Further observations on the immunological induction of DNA synthesis in mouse peritoneal macrophages. Role of products of activated lymphocytes.
Subcutaneous injection of ovalbumin (OA) into mice immunized with OA in Freund's complete adjuvant was followed by an increase in the numbers of peritoneal macrophages synthesizing DNA, determined by autoradiography. The effect was immunologically specific. The increase was followed by an increase in the numbers of peritoneal macrophages; the numbers of peritoneal lymphocytes also increased. Injection of OA into immunized or normal mice was followed by a blood monocytosis. Increased DNA synthesis, determined by liquid scintillation counting, occurred in spleen or lymph node cells from immunized mice, cultured with OA. Diluted supernatants from such cultures, injected intravenously into normal mice, caused increases in the numbers of DNA-synthesizing peritoneal macrophages. Similarly, supernatants from concanavalin A stimulated spleen cells, freed of Con A, also caused an increase in DNA-synthesizing macrophages.
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology*; D001345:Autoradiography; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D007271:Injections, Intradermal; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D010047:Ovalbumin / Q000008:administration & dosage / Q000494:pharmacology; D012709:Serum Albumin / Q000008:administration & dosage; D013997:Time Factors
D008222:Lymphokines; D012709:Serum Albumin; D003208:Concanavalin A; D010047:Ovalbumin; D004247:DNA