[Cellular distribution of elongation factor EF1 in wheat germ].
Cellular distribution of elongation factors (EF1) from imbibed then redessicated wheat embryos is determined after purification and analytical gel electrophoresis of soluble and ribosome-bound factors. Two heavy forms (EF1 H, mol. wt, 250 000) are found in cytosol while ribosome-bound factors contain a light form (EF1L, mol. wt, 45 000) with the greatest activity and a heavy form (mol. wt, 160 000) which might well be an intermediary in the recycling of ribosomal factor EF1L to soluble factor EF1H.
D002845:Chromatography; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D006882:Hydroxyapatites; D008970:Molecular Weight; D010445:Peptide Elongation Factors / Q000302:isolation & purification*; D012270:Ribosomes / Q000201:enzymology; D012639:Seeds / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology; D014908:Triticum / Q000201:enzymology
D006882:Hydroxyapatites; D010445:Peptide Elongation Factors
Distribution cellulaire des facteurs d'élongation EF1 du germe de blé