Analysis of the fibrin-polymerizing reaction using sodium dodecylsulfate-agarose gel electrophoresis.
1. A new method of sodium dodecylsulfate gel electrophoresis, sodium dodecyl-sulfate-agarose gel electrophoresis, was developed. The new electrophoresis was shown to have a high and efficient resolving power for fibrin polymers and was also applicable to other proteins. 2. By sodium dodecylsulfate-agarose gel electrophoresis, the fibrin polymerizing reaction with activated fibrin stabilizing factor was analyzed in detail. The cross-linking between the gamma-chains was indicated to occur at an intermolecular level. The solubility of polymerized fibrin was suggested to be related mainly to the formation of the crosslinks between gamma-chains.
D000818:Animals; D001777:Blood Coagulation; D002417:Cattle; D004586:Electrophoresis / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005176:Factor XIII; D005337:Fibrin; D046911:Macromolecular Substances; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D011108:Polymers; D011487:Protein Conformation; D012685:Sepharose; D012967:Sodium Dodecyl Sulfate
D046911:Macromolecular Substances; D010446:Peptide Fragments; D011108:Polymers; D012967:Sodium Dodecyl Sulfate; D005337:Fibrin; D012685:Sepharose; D005176:Factor XIII