A simple and sensitive micro-haemagglutination inhibition assay for the semi-quantitation of the third component of complement.
C3-coated cells have been prepared by incubating sensitised erythrocytes with the appropriate dilutions of serum. Inhibition of agglutination of these cells by an anti-C3 serum provides a sensitive and specific assay for C3 detection and semi-quantitation.
D000818:Animals; D002021:Buffers; D003176:Complement C3 / Q000032:analysis* / Q000302:isolation & purification; D003165:Complement System Proteins / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology; D006023:Glycoproteins; D006385:Hemagglutination Inhibition Tests / Q000379:methods*; D006460:Hemolysin Proteins; D006801:Humans; D012756:Sheep / Q000276:immunology
D002021:Buffers; D003176:Complement C3; D006023:Glycoproteins; D006460:Hemolysin Proteins; D003165:Complement System Proteins