The retrograde transport of horseradish peroxidase from the developing limb of the chick embryo.
Chick embryos ranging in age from 4.0 to 18 days of incubation and 1-2-day-old hatchlings received injections of HRP solutions directly into the leg musculature. After survival periods of from 5 to 25 h motoneurons and cells in the spinal sensory ganglia were found to be stained with the HRP reaction product. It was found that the first appearance of a positive HRP reaction coincided with the time when nerve processes are first detected in the limb-bud by silver techniques (i.e. at 4.5 days of incubation). Only neurons with processes in the region of injection showed a positive reaction. The demonstration of a retrograde transport mechanism in neurons and axons which are still undergoing growth and differentiation provides a possible mechanism for the peripheral regulation of certain features of CNS neurogenesis. The application of this technique during development may also allow one to map neuroanatomical pathways during their formation.
D000818:Animals; D002642:Chick Embryo; D005727:Ganglia, Spinal / Q000196:embryology* / Q000378:metabolism; D006614:Hindlimb / Q000294:innervation*; D006651:Histocytochemistry; D010544:Peroxidases / Q000378:metabolism; D013127:Spinal Nerves / Q000196:embryology* / Q000378:metabolism
D010544:Peroxidases