Quantitative assays of human monocyte-macrophage function.
Monocyte-macrophages are required for the development of cell mediated immunity to a variety of microorganisms and tumors. Quantitative assays of human monocyte-macrophage function would be most useful in the evaluation of cell mediated immune function in man. Five quantitative assays are described that provide a human monocyte-macrophage function profile. These assays parallel the physiologic steps necessary for monocyte-macrophages to function as phagocytes: 1) chemotaxis, 2) opsonization, 3) phagocytosis, 4) phagocytosis-induced metabolic stimulation and 5) destruction of foreign material. Application of these quantitative assays will allow detection and dissection of disorders of monocyte-macrophage function in man.
D000328:Adult; D001433:Bacteriolysis; D002469:Cell Separation; D002633:Chemotaxis; D006801:Humans; D007111:Immunity, Cellular; D008264:Macrophages / Q000276:immunology* / Q000502:physiology; D009000:Monocytes / Q000276:immunology* / Q000502:physiology; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D009895:Opsonin Proteins; D010587:Phagocytosis / Q000187:drug effects; D015003:Yeasts / Q000378:metabolism
D009895:Opsonin Proteins; D009580:Nitroblue Tetrazolium