Carbohydrate-binding specificity of the so-called galactose-specific phytohemagglutinins.
The carbohydrate-binding specificities of various so-called galactose-specific phytohemagglutinins were investigated by means of hemagglutination-inhibition assays. As hapten inhibitors, glycopeptides prepared by pronase-digestion of various glycoproteins (porcine submaxillary mucin, bovine submaxillary mucin, and porcine thyroglobulin), and several glycosides of D-galactose and 2-acetamido-2-deoxy-D-galactose were employed. The results indicate that these galactose-specific phytohemagglutinins may recognize the sugar residue penultimate to D-galactose or 2-acetamido-2-deoxy-D-galactose residues of the sugar chain with which they interact, and that they can be classified into three groups based on the type of sugar sequence which they primarily recognize.
D000818:Animals; D002417:Cattle; D005688:Galactosamine / Q000276:immunology; D005690:Galactose / Q000276:immunology*; D005696:Galactosidases; D006020:Glycopeptides / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D037102:Lectins; D009077:Mucins / Q000276:immunology; D009439:Neuraminidase; D011485:Protein Binding; D013329:Structure-Activity Relationship; D013363:Submandibular Gland / Q000032:analysis; D013552:Swine; D013954:Thyroglobulin / Q000276:immunology
D006020:Glycopeptides; D006023:Glycoproteins; D037102:Lectins; D009077:Mucins; D005688:Galactosamine; D013954:Thyroglobulin; D005696:Galactosidases; D009439:Neuraminidase; D005690:Galactose