Adenovirus type 2 DNA replication. II. Termini of DNA replication.
Complete, mature adenovirus type 2 DNA molecules were isolated from virus-infected HeLa cells, pulse-labeled at 20 h postinfection in [3H]thymidine pulses shorter than the time necessary for one round of viral DNA replication. After digestion with the restriction endonucleases Eco RI, Hpa I, and Hind III, a temporal order of synthesis of different regions of the viral genome was established from the relative specific radioactivities in the restriction enzyme fragments. A comparison with the physical order of these fragments revealed the existence of two termini of DNA replication towards both the molecular right and left ends, respectively, of the viral chromosome.
D000256:Adenoviridae / Q000032:analysis / Q000378:metabolism*; D001483:Base Sequence; D004261:DNA Replication; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D006367:HeLa Cells
D004279:DNA, Viral; D004262:DNA Restriction Enzymes