A comparative study of the effects of aflatoxin B1, alpha-amanitin and actinomycin-D on RNA synthesis by rat liver.
The effect of different combinations of aflatoxin B1, alpha-amanitin and actinomycin-D on the incorporation of orotic acid-6-14C into RNA was investigated using rat liver slices. The results support the view that the mode of action of aflatoxin B1 and alpha-amanitin are similar in some respects, and that aflatoxin B1 and actinomycin-D act according to different mechanisms. Evidence was also obtained about the formation of an active metabolite of aflatoxin B1 in this system.
D000348:Aflatoxins / Q000494:pharmacology*; D000546:Amanitins / Q000494:pharmacology*; D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009963:Orotic Acid / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism
D000348:Aflatoxins; D000546:Amanitins; D003609:Dactinomycin; D009963:Orotic Acid; D012313:RNA