Renal handling of human beta2-microglobulin in the rat: the importance of sham-operation.
The plasma disappearance rates of human beta2-microglobulin and (hydroxy-methyl-C14)-inulin was measured in unoperated, sham-operated, ureter-ligated and nephrectomised rats. Judging from the C14-activity curves in ureter-ligated and nephrectomised rats a substantial glomerular filtration continued after obstruction of the urine flow. Almost 90% of the injected protein had disappeared from plasma 120 min after injection into nephrectomised rats indicating a considerable extra-renal elimination and invalidating attempts to evaluate different renal mechanisms of elimination before and after ureter-ligation. Sham-operation induced an increase of the plasma disappearance of both beta2-microglobulin and inulin. The latter phenomenon may explain earlier findings of similarity between the disappearance curves of low molecular weight proteins in unoperated and ureter-ligated animals and stresses the importance of using sham-operated animals as controls.
D000818:Animals; D005919:Glomerular Filtration Rate; D006801:Humans; D007275:Injections, Intravenous; D007444:Inulin / Q000008:administration & dosage / Q000378:metabolism; D007668:Kidney / Q000378:metabolism* / Q000502:physiology; D008026:Ligation; D008297:Male; D009392:Nephrectomy; D051381:Rats; D012712:Serum Globulins / Q000008:administration & dosage / Q000378:metabolism*; D013514:Surgical Procedures, Operative; D013997:Time Factors; D014513:Ureter / Q000502:physiology
D012712:Serum Globulins; D007444:Inulin