Practical aspects on the determination of serum alkaline phosphatase isoenzymes using L-phenylalanine and urea.
Simple methods for estimation of the most frequently occurring alkaline phosphatase isoenzymes in serum are evaluated. Methods based on inhibition by L-phenylalanine or denaturation by heat or urea have been adopted for a reaction-rate instrument (LKB 8600) using conditions recommended by the Scandinavian Enzyme Committee, when applicable. The analytical results are directly evaluated for various groups of patients and also used in a mathematical "three-isoenzyme" model. These calculations did not improve the clinical value of the primarily obtained data. Thus, it was concluded that under certain precautions the results obtained in the presence of L-phenylalanine or urea should be used separately.
D000469:Alkaline Phosphatase / Q000097:blood*; D001859:Bone Neoplasms / Q000201:enzymology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006358:Hot Temperature; D006801:Humans; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000097:blood; D008099:Liver / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology; D010649:Phenylalanine; D011247:Pregnancy; D014508:Urea
D007527:Isoenzymes; D010649:Phenylalanine; D014508:Urea; D000469:Alkaline Phosphatase