Poly (U, s 4-U) as a synthetic messenger RNA for polyphenylalanine synthesis in a cell-free system derivem derived from rat liver.
Two different U and S4-U containing polymers with U:s4U ratio of 3:1 and 5:1 were synthesized. Their activity as messenger RNA was studied in an amino acid incorporation cell-free system from rat liver. It was shown that both copolymers can stimulate the incorporation of phenylalanine into oligophynylalamyl-tRNA.
D000818:Animals; D001665:Binding Sites; D002474:Cell-Free System; D008099:Liver / Q000378:metabolism*; D009842:Oligopeptides / Q000096:biosynthesis; D010452:Peptide Biosynthesis; D010649:Phenylalanine / Q000378:metabolism; D011072:Poly U / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D051381:Rats; D012270:Ribosomes / Q000378:metabolism; D013889:Thiouracil
D009842:Oligopeptides; D012333:RNA, Messenger; D011072:Poly U; D010649:Phenylalanine; D013889:Thiouracil; D012343:RNA, Transfer