Quantitative infrared spectroscopy of lipids in solution: I. Theoretical and practical aspects of the new method.
In Part I of this series, new data for quantitative infrared spectroscopy of pure lipids are reported for various frequency regions. A new analysis of previously reported infrared data demonstrates that the E1 percent/1 cm concept, long in use in ultraviolet studies of proteins, is applicable to the analysis of lipids of the infrared. It is shown that integrated absorbance measurements over optimal limits of the C-H-stretching region are substantially more sensitive than those of the C-H-bending region. It is shown that the new method is more sensitive and specific than gravimetric methods currently in use.
D005989:Glycerides / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008433:Mathematics; D013055:Spectrophotometry, Infrared / Q000379:methods; D013229:Stearic Acids / Q000032:analysis
D005989:Glycerides; D008055:Lipids; D013229:Stearic Acids