Diazepam, ethanol and drug metabolizing enzymes in rat liver.
The effect of diazepam and/or ethanol treatment of seven days on the drug metabolizing enzymes in rat liver microsomes was studied. Diazepam decreased the metabolism rate of 3,4-benzpyrene by 38% and that of p-nitrobenzoic acid by 15%. Ethanol increased the metabolism rate of N-methylaniline by 15% and decreased the reduction of p-nitrobbenzoic acid by 48%. Although diazepam alone had no inducing effect, its use with ethanol increased the cytochrome P-450 content (56%) and the rate of metabolism of N-methylaniline (44%) more than ethanol alone. The rate of metabolism of diazepam was not affected by any treatment.
D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003975:Diazepam / Q000378:metabolism / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D009579:Nitrobenzoates / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010084:Oxidation-Reduction; D010089:Oxidoreductases, N-Demethylating; D051381:Rats
D000814:Aniline Compounds; D009579:Nitrobenzoates; D000431:Ethanol; D003577:Cytochrome P-450 Enzyme System; D001579:Benzopyrene Hydroxylase; D010089:Oxidoreductases, N-Demethylating; D003975:Diazepam