Fractionation of some bovine whey proteins by recycling gel filtration on a large scale.
Fractionation of bovine whey concentrate was performed by gel filtration on Sephadex G-75 both on a laboratory scale and on a large scale. By a recycling procedure and improved separation was obtained and the whey proteins were resolved into four fractions in the weight ratio 3:12:1:4. The fractions were analysed by polyacrylamide gel (PAG) electrophoresis and the apparent molecular weights were determined by thin layer gel chromatography (TLG) and by sodium dodecylsulfate (SDS) gel electrophoresis.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008722:Methods; D008894:Milk Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D012967:Sodium Dodecyl Sulfate
D008894:Milk Proteins; D012967:Sodium Dodecyl Sulfate