Hydrolysis of biological materials for histamine determination on the isolated guinea-pig ileum.
Conditions of hydrolysis of biological material for determinations of histamine (Hi) using the guinea-pig ileum method were investigated. Overheated vapour has been applied at varying intervals and it was found that optimal conditions for tissue He liberation are obtained after 2 hour hydrolysis in autoclave. Results were compared with the orginal Code's method.
D000818:Animals; D006168:Guinea Pigs; D006632:Histamine / Q000032:analysis*; D006868:Hydrolysis; D007082:Ileum / Q000032:analysis*; D008722:Methods; D013997:Time Factors
D006632:Histamine