Differentiation of Friend virus-induced leukemia cells.
A long-term cultured Friend leukemia cell is able to differentiate along the erythrocytic series following treatment with some substances and that can be demonstrated by a conjugated erythrocyte membrane-specific antibody technique. Differentiation is induced by inhibition of DNA synthesis and by dimethylsulfoxide (DMSO), erythropoietin (EP) and Vitamin B12 (B12), and other agents. The effective substances were divided into 2 groups by the mode of differentiation of Friend cells. A differentiated state continues for several days when cells are re-cultured in the medium without substances. The decreased tumorigenecity of differentiated cells is proved by back transplantation to mice.
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004900:Erythroblasts / Q000473:pathology*; D004912:Erythrocytes / Q000473:pathology*; D004921:Erythropoietin / Q000494:pharmacology; D005622:Friend murine leukemia virus; D007942:Leukemia, Experimental / Q000097:blood* / Q000209:etiology / Q000378:metabolism; D051379:Mice; D051381:Rats; D013154:Spleen; D013997:Time Factors; D014805:Vitamin B 12 / Q000494:pharmacology
D000963:Antimetabolites; D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D004921:Erythropoietin; D014805:Vitamin B 12; D004121:Dimethyl Sulfoxide