Covalent linkage between RNA and nascent DNA in the slime mold, Physarum polycephalum.
When alpha--32 P-labeled deoxyribonucleoside triphosphates are injected into plasmodia of the eukaryotic slime mold, Physarum polycephalum, they are incorporated initially into strands of DNA which are mostly less than 300 nucleotides long. Sixty minutes after injection incorporated deoxyribonucleoside triphosphates are found in much longer strands. If the short strands found two minutes after injection are denatured and centrifuged to equilibrium in a Cs(2)SO(4) density gradient, they migrate to a density slightly greater than that of single-stranded Physarum DNA. When these short strands are treated with alkali to hydrolyze RNA, a small fraction of the incorporated -32P is made acid-soluble and is identified as a mixture of the four ribonucleoside 2',3'-monophosphates. Such transfer of -32P to ribonucleotides occurs when any of the 4 alpha--32P-labeled deoxyribonucleoside triphosphates is used for injection, but the transfer is greatest with [alpha--32P]dGTP. We conclude that very short stretches of RNA are found linked through phosphodiester bonds to nascent DNA chains in Physarum polycephalum and that any of the 16 possible combinations of ribo- and deoxyribonucleotides can occur at the RNA-DNA junction.
D001483:Base Sequence; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification; D004590:Electrophoresis, Paper; D009235:Myxomycetes / Q000032:analysis* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D012265:Ribonucleotides / Q000032:analysis
D012265:Ribonucleotides; D012313:RNA; D004247:DNA