[Attempts at localisation and evaluation of the RNA amounts in the tail and the cytoplasmic droplet of bull spermatozoa].
The RNA has been located and proportioned by a spectrophotometric method which absorbs the ultra-violet light. The technique used allows us to execute punctual dosage into the spermatozoon's tail. Each of these punctual dosages makes us realise the RNA localisation; and the sum of these dosages makes us realise the total quantity which is contained in one spermatozoon. The signification of the collected data is debated. A comparison has been made with chemical methods. These methods give the advantage to obtain valuations on large quantities of spermatozoa. But these valuations are always global value, and the individual value is always a mean's expression. Without being dull, the microspectophotometry is slow and brings about to effect on much restricted numbers, but the data are all individual measurements, (and we can even obtain punctual measurements in the range of 400 to 500 for the tail only. So that we can interprete these individual measurements and make very interesting comparisons from cell to cell. The possibilities are therefore very large and they complete fortunately the chemical methods.
D008297:Male; D012313:RNA / Q000032:analysis*; D013053:Spectrophotometry; D013082:Sperm Tail / Q000032:analysis*; D013094:Spermatozoa / Q000032:analysis*
D012313:RNA
Essai de localisation et de dosage de l'ARN dans le fragelle et dans la gouttelette cytoplasmique du spetometry