Gangliosides of chemically and virally transformed rat embryo cells.
The gangliosides of control rat embryo cells, 3-methylcholanthrene, Rauscher leukemia virus, and combined 3-methylcholanthrene-Rauscher leukemia virus transformants were examined using [14 C]glucosamine as a tracer. All four cell lines exhibited a complex pattern of gangliosides. While N-acetylgalactosaminyl-(N-acetylneuraminyl)-galactosyl-glucosyl-ceramide was the major ganglioside in the control cell line, N-acetylneuraminyl-galactosyl-glucosyl-ceramide was the major ganglioside in the three transformants. The 3-methylcholanthrene transformant possessed a ganglioside pattern different from that of the Rauscher leukemia virus transformant. Hydrolysis of the gangliosides indicated that galactosamine, N-acetyl-and N-glycolylneuraminic acid were the labeled components in all cell lines.
D000116:Acetylgalactosamine / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002518:Ceramides / Q000096:biosynthesis; D004622:Embryo, Mammalian; D005690:Galactose / Q000032:analysis; D005732:Gangliosides / Q000096:biosynthesis*; D005944:Glucosamine / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D008748:Methylcholanthrene / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011925:Rauscher Virus / Q000378:metabolism; D012794:Sialic Acids / Q000032:analysis
D002518:Ceramides; D005732:Gangliosides; D012794:Sialic Acids; D008748:Methylcholanthrene; D005947:Glucose; D000116:Acetylgalactosamine; D005944:Glucosamine; D005690:Galactose