Heat denaturation of bovine liver glutamate dehydrogenase.
Heat denaturation of bovine liver glutamate dehydrogenase occurred at 47 degrees with loss of enzyme activity and formation of inactive, insoluble protein. Fractional loss of catalytic activity coincided with alteration in protein fluorescence and solubility for a corresponding percentage of protein molecules. Operationally, at 50% denaturation, one-half of the total population of enzyme molecules is fully active catalytically and soluble and the other half of the protein molecule population is completely inactive catalytically and insoluble.
D000818:Animals; D002417:Cattle; D005969:Glutamate Dehydrogenase; D006358:Hot Temperature; D008099:Liver / Q000201:enzymology*; D008968:Molecular Conformation; D011489:Protein Denaturation; D011506:Proteins / Q000032:analysis
D011506:Proteins; D005969:Glutamate Dehydrogenase