[Effect of low temperatures on the level of nucleic acids and protein in the nuclei of early bird embryos (cytophometric study)].
The content of DNA and protein in the nuclei was determined cytophotometrically as well as DNA and RNA in nucleoli of chicken embryos of different gastrullation stages after 3 days of storage at +6 degrees, +2 degrees and - 2 degrees C. It was established that the response of embryos to cold at the cell level became apparent in inhibiting the protein synthesizing system (under effects of suboptimal and extreme temperature). inhibiting the migration processes of nuclear RNA into the nucleoplasm and nuclear proteins into the cytoplasm and a certain alteration in the nucleus ploidy (under extreme conditions). The reaction of each stage under study and different cellular layers of the same differentiation stage were shown to have certain specificity.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002642:Chick Embryo / Q000378:metabolism*; D003080:Cold Temperature; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D005865:Gestational Age; D011003:Ploidies; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*
D011506:Proteins; D012313:RNA; D004247:DNA
Vliianie nizhkikh temperatur na soderzhanie nukleinovykh kislot i belka v iadrakh rannikh zarodysheĭ ptits (tsitofotometricheskoe issledovanie)