Effect of leucine on the temperature sensitive phenotype of a mammalian leucyl-tRNA synthetase mutant.
The concentration of leucine in the growth medium has been found to influence the expression of the temperature sensitive phenotype of a mutant of Chinese hamster ovary cells with an altered leucyl-tRNA synthetase. Plating efficiency and growth studies showed that increasing the leucine concentration allows cells to survive at normally non-permissive high temperatures and conversely decreasing the leucine concentration enhances the adverse effectsof high temperature. A similar but smaller effect was noted with isoleucine. It is suggested that this observation may form the basis of a rapid test, useful in directing the investigation of the lesion in similar mutants to pathways involving specific amino acids.
D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D005260:Female; D007532:Isoleucine / Q000494:pharmacology; D007930:Leucine / Q000494:pharmacology*; D009154:Mutation; D010053:Ovary; D010641:Phenotype; D013696:Temperature
D007532:Isoleucine; D000604:Amino Acyl-tRNA Synthetases; D007930:Leucine