Application of pulsed-gradient Fourier transform nuclear magnetic resonance to the study of self-diffusion of phospholipid vesicles.
A pulsed-gradient Fourier transform nuclear magnetic resonance (NMR) technique was appplied to the study of diffusion of phospholipid vesicles. The diffusion coefficient of dimyristoyllecithin vesicles (DML) in a D2O-phospahte buffer at 37 degrees is D = 1.9 TIMES 10(-6) cm2/sec. In a solution made viscous by DNA addition, the diffusion coefficient of DML vesicles was 3.5 times 10(-7) cm2/sec. These values compare favorably with the diffusion rate for liposomes as determined by ultracentrifugation and by Stokes law calculation. The data suggest that DML diffusion is controlled primarily by whole liposome migration as opposed to movement of individual molecules within the liposome, liposome rotation, or fast exchange between lecithin molecules in solution and in vesicles.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004247:DNA; D004058:Diffusion; D005583:Fourier Analysis; D007700:Kinetics; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D008433:Mathematics; D008968:Molecular Conformation; D010713:Phosphatidylcholines; D013950:Thymus Gland
D008081:Liposomes; D010713:Phosphatidylcholines; D004247:DNA