Acyl-CoA pool and acyl-CoA thioesterase in Streptomyces noursei var. polifungini.
The size of acyl-CoA pool in S. noursei var. polifungini was found to be associated with the antibiotic-synthesizing ability, and was negatively correlated with both the thio-esterase activity (acetyl-CoA hydrolase, EC 3.1.2.1) and its affinity towards acetyl-CoA and propionyl-CoA. The apparent Michaelis constants with acetyl-CoA and propionyl-CoA were 33 times 10(-5) and 6.6 times 10(-5) M in the low producing strain, and 8.5 times 10(-4) and 1.2 times 10(-4) M in the high producing strain, respectively.
D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000935:Antifungal Agents / Q000096:biosynthesis*; D003065:Coenzyme A / Q000031:analogs & derivatives*; D011090:Polyenes / Q000096:biosynthesis; D013045:Species Specificity; D013302:Streptomyces / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000378:metabolism*
D000935:Antifungal Agents; D011090:Polyenes; D000105:Acetyl Coenzyme A; D013869:Thiolester Hydrolases; D003065:Coenzyme A