The incorporation of radioactive uridine into the hepatitis B antigen of a chimpanzee.
Radioactive (3H) uridine was incorporated into RNA isolated from the HBsAg of a chimpanzee carrier. HBsAg was purified by precipitation as an immune complex with the IgG fraction of chimpanzee anti-HBs. RNA was extracted from the washed complex with buffered phenol precipitated with alcohol and four nucleotides were identified by thin layer chromatography after alkali degradation.
D000818:Animals; D006511:Hepatitis B Antigens / Q000378:metabolism*; D008297:Male; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D014529:Uridine / Q000378:metabolism*
D006511:Hepatitis B Antigens; D012367:RNA, Viral; D014529:Uridine