Cortisol-binding protein in the cytosol of rat carrageenin granuloma.
Cortisol-binding protein was prepared and partially purified by (NH4)2SO4 fractionation and DEAE-cellulose column chromatography from 105,000 g supernatant fraction of cytoplasm in rat carrageenin granuloma, which is assumed to be one of the most appropriate experimental models of inflammation. The cortisol-binding protein in the inflammatous tissue, although similar to transcortine, was not transcortine itself. The binding protein was eluted at 0.12 M NaCl by DEAE-cellulose column chromatography with a shallow salt gradient. Sedimentation constant and dissociation constant of the binding protein were 4-5 S and 1.0 X 10(-9) M, respectively. Optimum pH for binding to cortisol was 8.0. Binding ability of the binding protein to cortisol was very sensitive to pronase E and trypsin but resistant to RNase. Specificity of the protein for binding other steroids revealed that 17beta-estradiol did not bind to the protein, while androstenedione and testosterone had one sixth as much affinity to the binding protein as that of cortisol. There was good a correlation between the amount of the binding protein in the inflammatory tissue and anti-inflammatory effect of cortisol. Namely, the maximum cortisol-binding ability was seen on a 5 day old granuloma which is the so called 'steroid sensitive stage'. Thereafter, the binding ability decreased with the increasing stage of granuloma.
D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D002351:Carrageenan; D002848:Chromatography, DEAE-Cellulose; D003600:Cytosol / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D006099:Granuloma / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D011402:Pronase / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism; D014156:Transcortin / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol; D002351:Carrageenan; D014156:Transcortin; D012260:Ribonucleases; D014357:Trypsin; D011402:Pronase