Utilization of ribonucleic acid and deoxyoligomer primers for polyadenylic acid synthesis by adenosine triphosphate: polynucleotidylexotransferase from maize.
The ATP:polynucleotidylexotransferase isolated and purified from maize seedlings catalyzes the synthesis of polyadenylic acid by the sequential addition of 80 to 200 AMP moieties from ATP to the 3'-hydroxyl terminus of either ribo- or deoxyoligomers. Copurification of the RNA and DNA-primed activities, identical metal cofactor and reaction requirements for either primer and identical heat inactivation curves with either primer strongly suggest that both primers are utilized by the same enzyme.
D000255:Adenosine Triphosphate; D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D003854:Deoxyribonucleotides; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D007700:Kinetics; D009713:Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D009841:Oligonucleotides / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism*; D011119:Polynucleotides / Q000096:biosynthesis*; D012313:RNA / Q000378:metabolism*; D013950:Thymus Gland; D013997:Time Factors; D003313:Zea mays / Q000201:enzymology
D003854:Deoxyribonucleotides; D009841:Oligonucleotides; D011119:Polynucleotides; D012313:RNA; D000255:Adenosine Triphosphate; D009713:Nucleotidyltransferases; D011062:Polynucleotide Adenylyltransferase