Replication of mitochondrial DNA. Selective inhibition of the H-strand synthesis in isolated mitochondria by sibiromycin.
Sibiromycin, an antitumour antibiotic forming a stable complex with the double-stranded DNA selectively inhibits the incorporation of [3H]thymidine into the H-strand of mtDNA during the incubation of isolated rat liver mitochondria in vitro. A model accounting for this result is presented. It is concluded that the H-strand is a leading strand throughout mtDNA replication adn consequently the replication of rat liver monomer mtDNA is unidirectional.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D004261:DNA Replication / Q000187:drug effects*; D004272:DNA, Mitochondrial / Q000378:metabolism*; D003864:Depression, Chemical; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000378:metabolism; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D004272:DNA, Mitochondrial; D013936:Thymidine