The effect of a mammalian repair endonuclease on x-irradiated DNA.
DNA was extracted from rat liver of non-irradiated animals, and was irradiated in vitro, and from animals which received whole body doses of X-radiation. Sedimentation on neutral and alkaline sucrose gradients as well as measurements of 32P release after sequential treatment with endonuclease and alkaline phosphatase and determination of triphosphate incorporation after the sequential treatment with endonuclease, alkaline phosphatase and DNA polymerase indicated that DNA irradiated in vivo and in vitro were effective substrates for the mammalian repair endonuclease. The experiments suggest that in addition to strand breaks, X-radiation causes base damage and they have provided a plausible explanation for the formation of double strand breaks in DNA irradiated in vivo.
D000469:Alkaline Phosphatase; D000818:Animals; D004247:DNA / Q000528:radiation effects*; D004254:DNA Nucleotidyltransferases; D004260:DNA Repair / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004720:Endonucleases / Q000378:metabolism*; D008099:Liver / Q000528:radiation effects; D011118:Polynucleotide Ligases / Q000378:metabolism*; D011830:Radiation Effects; D051381:Rats; D014965:X-Rays
D004247:DNA; D004254:DNA Nucleotidyltransferases; D004720:Endonucleases; D000469:Alkaline Phosphatase; D011118:Polynucleotide Ligases