The composition and proposed subunit structure of egg-white beta-ovomucin. The isolation of an unreduced soluble ovomucin.
1. New preparations of reduced carboxymethylated beta-ovomucin (S-carboxymethyl-beta-ovomucin) were homogeneous by sedimentation analysis, analytical sedimentation to equilibrium in CsCl gradients, and disc electrophoresis in sodium dodecyl sulphate. 2. Degradation of S-carboxymethyl-beta-ovomucin with either CNBr or trypsin indicated the presence of a subunit (approx. mol. wt. 112300). 3. Electron microscopy showed that S-carboxymethyl-beta-ovomucin consisted of chains of globular units (approx. mol. wt. 103 000). IN 6M-guanidinium chloride S-carboxymethyl-beta-ovomucin existed mainly as an aggregate (mol. wt. 720 000). 4. S-Carboxymethyl-beta-ovomucin contained ester sulphate (4.24%, W/W) and carbohydrate (60%, W/W), which consisted of large amounts of galactose (22%, W/W), galactosamine (8.9%, W/W) and sialic acid (10.6%, W/W). 5. An unreduced soluble fibrous component (component SGH) extracted from crude ovomucin precipitate with 5M-guanidinium chloride contained beta-ovomucin (approx. 70%, W/W). By using the Scheraga-Mandelkern equation the molecular weight of component SGH was calculated to be 11.5 times 10(6).
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002264:Carboxylic Acids; D002498:Centrifugation; D002645:Chickens; D003488:Cyanogen Bromide; D004527:Egg Proteins / Q000302:isolation & purification*; D004589:Electrophoresis, Disc; D004952:Esters / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005690:Galactose / Q000032:analysis; D008854:Microscopy, Electron; D008970:Molecular Weight; D010047:Ovalbumin / Q000032:analysis; D010059:Ovomucin / Q000032:analysis / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D012794:Sialic Acids / Q000032:analysis; D014357:Trypsin; D014783:Viscosity
D000596:Amino Acids; D002241:Carbohydrates; D002264:Carboxylic Acids; D004527:Egg Proteins; D004952:Esters; D012794:Sialic Acids; D010059:Ovomucin; D005688:Galactosamine; D010047:Ovalbumin; D014357:Trypsin; D003488:Cyanogen Bromide; D005690:Galactose