[Modified method of ultramicroelectrophoretic protein fractionation].
A modification is described for the method of ultramicroelectrophoretic fractioning of proteins in polyacrylamide gel in capillaries 400--100 microns in diameter. The original construction of the assembly of apparatuses is offered which can be assembled from details made in the USSR. The operation of dosed filling of capillaries is significantly facilitated. The optimal quantity of protein in one sample reaches 1.5--0.1 mkg (depending on the capillary diameter). The reproducability and sharpness of protein fractions by the offered method are not worse compared with the generally accepted method of macro-disc electrophoresis.
D002458:Cell Fractionation / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D008832:Microchemistry / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D014586:USSR
D011506:Proteins
Modifitsirovannyĭ metod ul'tramikroélektroforeticheskogo fraktsionirovanii belkov