Effect of insulin on human intestinal smooth muscle.
The action of insulin (0.1 U/ml) on the metabolism of human intestinal smooth muscle was studied in vitro. The experiments were performed on the muscle layer of human jujunum obtained from patients undergoing intestinal shunt operations because of obesity. Insulin significantly increased glucose uptake, glycogen content, the membrane transport of alpha-amino-isobutyric acid (AIB), the incorporation of leucine into protein and tended to increase the membrane transport of the nonutilizable model monosaccharide 3-0-methylglucose. The effects of insulin were moderate and appeared after incubation times of 120 to 180 min.
D000328:Adult; D000621:Aminoisobutyric Acids / Q000378:metabolism; D001693:Biological Transport, Active; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008297:Male; D008757:Methylglucosides / Q000378:metabolism; D008875:Middle Aged; D009124:Muscle Proteins / Q000096:biosynthesis; D013237:Stereoisomerism; D013395:Sucrose / Q000378:metabolism
D000621:Aminoisobutyric Acids; D007328:Insulin; D008757:Methylglucosides; D009124:Muscle Proteins; D013395:Sucrose; D007930:Leucine; D005947:Glucose