Preparation of coenocytes for freeze-etching.
Successful freeze-etching of a coenocyte has been accomplished with glutaraldehyde stabilization followed by infiltration with cryoprotectant. Hyphae of the coenocytic water mold Achlya were stabilized with 5% glutaraldehyde in phosphate buffer. Gradual infiltration by dropwise addition of the cryoprotectant (25% glycerol, 10% ethylene glycol, distilled water, v/v) is accomplished over a period of 8-10 hr on a shaker. Subsequent freeze-etching is carried out by standard procedures.
D002462:Cell Membrane / Q000648:ultrastructure; D002473:Cell Wall / Q000648:ultrastructure; D003451:Cryoprotective Agents; D003593:Cytoplasm / Q000648:ultrastructure; D005026:Ethylene Glycols; D005613:Freeze Etching; D005658:Fungi / Q000648:ultrastructure; D005990:Glycerol; D010944:Plants / Q000648:ultrastructure*
D003451:Cryoprotective Agents; D005026:Ethylene Glycols; D005990:Glycerol