Regulation of species-specific ribosomal RNA in a somatic hybrid cell.
When 13B hamster-mouse hybrid cells are harvested either right after 4 h of incubation with [me-3H]methionine or following 26 h of "chase" with excess non-radioactive methionine, in both cases about half of the labeled cytoplasmic rRNA is of hamster type. It had been previously shown in this laboratory (Eliceiri, G.L. (1973) Biochim. Biophys. Acta 312, 737-741) that when [3H]uridine was the radioactive precursor about 80% of the labeled cytoplasmic rRNA was of hamster type after a short incubation, and about half after a long incubation. It is postulated that a temporary difference in the specific acitivity of [3H]UTP in possibly segregated mouse and hamster types of nucleoli might account for these results. The master/mouse ratio of cytoplasmic rRNA in hybrid 13B is similar in free and in membrane-bound ribosomes, and in ribosomes of sparse (rapidly growing) cell populations and of confluent (slowly growing) cells.
D000818:Animals; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006822:Hybrid Cells / Q000378:metabolism*; D007553:Isotope Labeling; D008715:Methionine / Q000378:metabolism; D051379:Mice; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D012335:RNA, Ribosomal; D014316:Tritium; D008715:Methionine