[The effect of actinomycin d, acridine dyes and related substances on the biosynthesis of nucleic acids in normal and leukemic white blood cells. Comparative investigation in intact cells and in a cell-free system (author's transl)].
Acridine dyes inhibit the incorporation of 3H-thymidine and 3H-uridine in intact cells to the same extent as Actinomycin D. In contrast to Actinomycin D, RNA synthesis by DNA - dependent RNA polymerase in a cell-free system is inhibited at lo2 higher concentrations of acridine dyes, only. Possible differential effects on the cell membrane resulting in decreased intracellular pools of uridine and thymidine are discussed.
D000166:Acridines / Q000494:pharmacology*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002474:Cell-Free System / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004273:DNA, Neoplasm / Q000096:biosynthesis; D012321:DNA-Directed RNA Polymerases; D003609:Dactinomycin / Q000494:pharmacology*; D005622:Friend murine leukemia virus; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009696:Nucleic Acids / Q000096:biosynthesis*; D011370:Proflavine / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D013347:Subcellular Fractions / Q000187:drug effects*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D000166:Acridines; D004273:DNA, Neoplasm; D009696:Nucleic Acids; D012334:RNA, Neoplasm; D014316:Tritium; D003609:Dactinomycin; D012313:RNA; D004247:DNA; D011370:Proflavine; D012321:DNA-Directed RNA Polymerases; D013936:Thymidine; D014529:Uridine
Nukleinsäurebiosynthese normaler und leukämischer Blutzellen. Die Wirkung von Actinomycin D, Acridinfarbstoffen und verwandten Substanzen auf intakte Zellen und auf ein zellfreies System