New azasteroidal antifungal antibiotics from Geotrichum flavo-brunneum. II. Isolation and characterization.
A novel group of antibiotics, comprising microbiologically-active structurally-related factors A25822A, B, D, H, L, M and N, produced by culturing Geotrichum flavo-brunneum NRRL 3862 under submerged aerobic fermentation conditions was isolated by extraction. The individual factors were separated and purified by chromatography and crystallization. The major factor, A25822B, a 15-aza-24-methylene-D-homocholestadiene is a white crystalline compound, C25H45NO. The antibiotics are highly active against fungi and marginally active against bacteria.
D000935:Antifungal Agents / Q000302:isolation & purification*; D001372:Aza Compounds / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002774:Cholestadienes / Q000302:isolation & purification*; D003500:Cyclization; D005285:Fermentation; D006718:Homosteroids / Q000302:isolation & purification; D003904:Mitosporic Fungi / Q000032:analysis*; D012995:Solubility
D000935:Antifungal Agents; D001372:Aza Compounds; D002774:Cholestadienes; D006718:Homosteroids