The amino acid sequence of plastocyanin from spinach. (Spinacia oleracea L.).
The amino acid sequence of spinach (Spinacia oleracea L.) plastocyanin was determined. It consists of a single polypeptide chain of 99 residues and has a sequence molecular weight of 10415. The sequence was determined by using a Beckman 890C automatic sequencer and by the dansyl--phenyl isothiocyanate analysis of peptides obtained by the enzymic digestion of purified CNBr fragments. Overlap through the two methionine residues was not shown. Sedimentation equilibrium in the ultracentrifuge gave a molecular weight for spinach plastocyanin of about 9000, in contrast with the value of 21000 reported previously by Katoh et al. (1962).
D000595:Amino Acid Sequence; D008970:Molecular Weight; D010940:Plant Proteins / Q000032:analysis*; D010944:Plants / Q000032:analysis*; D010970:Plastocyanin / Q000032:analysis*
D010940:Plant Proteins; D010970:Plastocyanin