An aseptic peritoneal cell collection technic for small laboratory animals.
Peritoneal exudate was collected by introducing a lavage medium (sterile saline or an equivalent) via syringe and needle into the peritoneal cavity of the anesthetized animal. The lavage medium was recovered by allowing it to flow through the needle into a sterile container. This method guards against contamination. Eighty to 90% of the initial lavage volume is retrieved.
D000818:Animals; D000830:Animals, Laboratory; D001202:Ascitic Fluid / Q000166:cytology*; D006224:Cricetinae; D006168:Guinea Pigs; D008722:Methods; D051379:Mice; D009339:Needles; D011817:Rabbits; D051381:Rats; D013594:Syringes; D007507:Therapeutic Irrigation