Inhibition of hemoglobin synthesis by pancreatic deoxyribonuclease in rabbit reticulocyte lysates.
A pancreatic deoxyribonuclease preparation, shown to be free of significant ribonuclease activity, inhibits hemoglobin synthesis in a rabbit reticulocytelysate. Preincubation with DNAase (15 min at 25 degrees C) is required to obtain a marked inhibitory effect (nearly 70%). It has been shown that DNAase does not significantly interfere with the elongation or termination steps of translation, but it seems to prevent reinitiation of globin polypeptide chains allowing polysome run-off. In particular, the formation of the 40S/met-tRNAmetf complex is greatly reduced in the DNAase-treated lysate. At the moment the mechanism by which DNAase inhibits initiation is not clear.
D000818:Animals; D002287:Carcinoma, Krebs 2 / Q000378:metabolism; D003851:Deoxyribonucleases / Q000494:pharmacology*; D005914:Globins / Q000096:biosynthesis; D006454:Hemoglobins / Q000096:biosynthesis*; D007700:Kinetics; D008297:Male; D010179:Pancreas / Q000201:enzymology; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism
D006454:Hemoglobins; D012333:RNA, Messenger; D005914:Globins; D003851:Deoxyribonucleases