Peanut agglutinin, a new mitogen that binds to galactosyl sites exposed after neuraminidase treatment.
Peanut agglutinin, purified by affinity chromatography, agglutinates lymphocytes from mouse, rat, guinea pig, and man only after their treatment with neuraminidase. However, it stimulates only neuraminidase-treated rat and human cells. A similar number cell surface receptors for peanut agglutinin was found on neuraminidase-treated rat and mouse lymphocytes although the latter cells were not stimulated by the lectin. Galactose specifically inhibited the agglutination and stimulation of lymphocytes by peanut agglutinin. Sequential treatment of lymphocytes with neuraminidase and beta-galactosidase markedly reduced the response of the cells to stimulation by peanut agglutinin, soybean agglutinin, and galactose oxidase. It is suggested that the same galactosyl residue may be the target for the initial step in triggering lymphocytes by the above mentioned mitogens.
D000372:Agglutination Tests; D000818:Animals; D010367:Arachis; D001666:Binding Sites, Antibody; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005690:Galactose / Q000276:immunology* / Q000494:pharmacology; D005696:Galactosidases / Q000494:pharmacology; D006168:Guinea Pigs; D006801:Humans; D037102:Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D009439:Neuraminidase / Q000378:metabolism*; D037121:Plant Lectins; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D037102:Lectins; D008934:Mitogens; D037121:Plant Lectins; D014316:Tritium; D003208:Concanavalin A; D005696:Galactosidases; D009439:Neuraminidase; D013936:Thymidine; D005690:Galactose