Ribosomal and informational ribonucleoprotein complexes of animal cells. Study on rat liver ribonucleic acids as constituents of ribonucleoprotein complexes by chromatography on nucleoprotein-celite columns.
A novel method of RNA fractionation has been developed. Nuclear and cytoplasmic rat liver RNA species were fractionated as constituents of corresponding ribonucleoprotein particles, which were previously adsorbed on a Celite-column by their protein component. The fractionation is based on a dissociation of the particles (linear concentration gradient of LiCl and urea with subsequent temperature gradient), which results in a gradual release of the RNA molecules from ribonucleoprotein complexes. Thus the fractionation is in accordance with the tightness of the RNA-protein bonds. A gradient elution of RNA from a nucleoprotein-Celite column permitted fractionation of both ribosomal and rapidly labelled non-ribosomal RNA. The latter, both nuclear and cytoplasmic, could be separated by chromatography on nucleoprotein-Celite columns into two main fractions (components I and V). In cytoplasmic RNA components I and V presumably correspond to mlRNA (messenger-like RNA of free cytoplasmic particles) and mRNA (template RNA associated with ribosomes) respectively.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D007274:Injections, Intraperitoneal; D008099:Liver / Q000032:analysis*; D008297:Male; D009698:Nucleoproteins; D011132:Polyribosomes / Q000032:analysis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D051381:Rats; D012261:Ribonucleoproteins; D012270:Ribosomes / Q000032:analysis
D009698:Nucleoproteins; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins; D012313:RNA