Differential sensitivity of low molecular weight DNA polymerase to sulfhydryl-blocking reagents.
Activity of a 2.5 S mouse myeloma DNA polymerase (termed DNA polymerase II) measured with either poly(rA) or poly(dA) as template did not require sulfhydryl-reducing reagents, but was sensitive to inhibition by p-hydroxymercuribenzoate and the sulfhydryl-alkylating reagent, N-ethylmaleimide; however, the activity was much more sensitive to inhibition by p-hydroxymercuribenzoate than by the sulfhydryl-alkylating reagent. The p-hydroxymercuribenzoate inhibition appeared to involve the mercurial portion of the p-hydroxymercuribenzoate molecule because HgCl2 was an equally effective inhibitor, while p-hydroxybenzoate had little effect upon enzyme activity. The p-hydroxymercuribenzoate inhibition was reversed by an equal concentration of the sulfhydryl-reducing reagent, dithiothreitol.
D000478:Alkylation; D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D002460:Cell Line; D002712:Chlorides / Q000494:pharmacology; D004254:DNA Nucleotidyltransferases / Q000037:antagonists & inhibitors*; D004229:Dithiothreitol / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D006880:Hydroxy Acids / Q000494:pharmacology; D006902:Hydroxymercuribenzoates / Q000494:pharmacology; D008628:Mercury / Q000494:pharmacology; D051379:Mice; D009101:Multiple Myeloma / Q000201:enzymology*; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D001565:Benzoates; D002712:Chlorides; D006880:Hydroxy Acids; D006902:Hydroxymercuribenzoates; D013439:Sulfhydryl Reagents; D004254:DNA Nucleotidyltransferases; D008628:Mercury; D005033:Ethylmaleimide; D004229:Dithiothreitol