Synthesis of immobilized flavin derivatives and their use in purification of chicken egg-white ovoflavoprotein.
Affinity adsorbents for flavoproteins were prepared by the covalent attachment of polyacrylamide and agarose to flavin derivatives linked through position N(3) of the flavin nucleus. 3-Carboxymethyl-FMN covalently linked to aminoalkyl substituted agarose was successfully used for the separation and purification of the apo form of the ovoflavoprotein from chicken egg white. High yields and high purities were achieved by two different isolation procedures employing the affinity adsorbent.
D000178:Acrylamides; D000818:Animals; D001059:Apoproteins / Q000302:isolation & purification; D001965:Bromides; D002021:Buffers; D002645:Chickens; D002846:Chromatography, Affinity; D004527:Egg Proteins / Q000302:isolation & purification*; D004529:Egg White; D005486:Flavin Mononucleotide; D005415:Flavins; D005420:Flavoproteins / Q000302:isolation & purification*; D008024:Ligands; D010059:Ovomucin / Q000302:isolation & purification; D011485:Protein Binding; D012685:Sepharose
D000178:Acrylamides; D001059:Apoproteins; D001965:Bromides; D002021:Buffers; D004527:Egg Proteins; D005415:Flavins; D005420:Flavoproteins; D008024:Ligands; D010059:Ovomucin; D005486:Flavin Mononucleotide; D012685:Sepharose