[Accumulation of p-aminohippuric acid and cyclopenthiazide in renal cortex slices from rats of various ages and their dependence on the energy supply].
The accumulation of p-aminohippuric acid (PAH) and cyclopenthiazide, two drugs of acidic character and extremely different physico-chemical properties, was determined in renal cortical slices of rats aged 5, 15, 33, 55, 105, and 240 days. PAH is accumulated in the cortical slices of 5- and 15-day-old animals to a lesser extent than in those of adult rats. Cyclopenthiazide is accumulated in much higher amounts than PAH in all age groups. The age dependence of cyclopenthiazide accumulation is not so pronounced as with PAH accumulation. There is no accumulation of PAH in the cortical slices of all age groups through active tubular transport, when the energy supply is inhibited by means of 2,4-dinitrophenol (DNP) or nitrogen 2 times bubbling (i.e. anaerobic incubation). By subsequent addition of DNP to the incubation medium or subsequent N2 atmosphere bubbling, an already existing PAH accumulation may be completely nullified. Contrary to PAH accumulation, cyclopenthiazide accumulation can be neither prevented nor abolished by inhibiting the energy supply.
D000367:Age Factors; D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D003518:Cyclopenthiazide / Q000378:metabolism*; D004140:Dinitrophenols / Q000494:pharmacology; D004232:Diuretics; D004734:Energy Metabolism / Q000187:drug effects; D007672:Kidney Cortex / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism; D051381:Rats; D049993:Sodium Chloride Symporter Inhibitors / Q000378:metabolism*
D000618:Aminohippuric Acids; D004140:Dinitrophenols; D004232:Diuretics; D049993:Sodium Chloride Symporter Inhibitors; D003518:Cyclopenthiazide
Die Akkumulation von p-Aminohippursäure und Zyklopenthiazid in Nierenrindenschnitten verschieden alter Ratten und ihre Abhängigkeit von der Energiebereitstellung