[Biosynthesis of glutathione. VI. Ocurrence and level of gamma-glutamylcysteine in human red cells (author's transl)].
The concentration of the glutathione precursor gamma-glutamylcysteine in hemolysates of human red cells was determined using three different methods. a) The [14C]N-ethylmaleimide derivatives of the intracellular sulfhydryl compounds were separated on a cation exchange column. The amount of [14C]N-ethylmaleimide glutamycysteine was radiochemically determined. b) With purified glutathione synthetase, an enzymatic endpoint metabolite determination was performed with [14C]glycine as one substrate. The amount of labelled glutathione formed from the gamma-glutamylcysteine present was measured radiochemically.
D003545:Cysteine / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism*; D005033:Ethylmaleimide / Q000097:blood; D005978:Glutathione / Q000096:biosynthesis / Q000097:blood*; D006801:Humans; D010453:Peptide Synthases / Q000097:blood
D010453:Peptide Synthases; D005978:Glutathione; D003545:Cysteine; D005033:Ethylmaleimide
Glutathionbiosynthese, VI. Der gamma-Glutamylcystein-Spiegel in menschlichen Erythrozyten