Separation of synthetic mixtures of sugars and of sugars in body fluids with a high-resolution carbohydrate analyzer.
With use of a specially designed gradient-generating system, of anion-exchange resins of 10-20 mum particle diameter, and of an orcinol solution in concentrated sulfuric acid (1 g/liter) as detection reagent, a mixture of 16 carbohydrates can be separated in less than 4 h. The limit of detection for most sugars is in the 0.1--2.0 nanomole range. The detection reagent is stable for months and does not become colored. The reagent mixes with the column effluent in an exothermic reaction and allows use of relatively short mixing coils. The analyzer is appropriate for investigating body fluids rapidly and with high sensitivity.
D002852:Chromatography, Ion Exchange / Q000295:instrumentation; D004187:Disaccharides / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000652:urine; D006801:Humans; D007202:Indicators and Reagents; D009005:Monosaccharides / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000652:urine; D012118:Resorcinols
D004187:Disaccharides; D007202:Indicators and Reagents; D009005:Monosaccharides; D012118:Resorcinols; C005282:orcinol