Haemoglobin synthesis in human fetal liver maintained in short-term tissue culture.
Human fetal liver maintained in tissue culture has been examined as a model system for studying the factors involved in the switch from fetal to adult haemoglobin production. In erythropoietin-treated cultures alpha-, beta- and gamma-chain synthesis remains active for up to 12 days. Globin-chain synthesis is balanced and the alpha/beta + gamma-chain production ratio remains constant up to about 8 days; in longer cultures gamma-chain production declines before that of beta chains. It is concluded that the system may be useful for examining factors involved in the switch from gamma- to beta-chain production provided that these are acting on cells already committed to the erythroid compartment.
D004305:Dose-Response Relationship, Drug; D004920:Erythropoiesis; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D005319:Fetal Hemoglobin / Q000096:biosynthesis; D005914:Globins / Q000096:biosynthesis; D006454:Hemoglobins / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000196:embryology* / Q000378:metabolism; D011247:Pregnancy; D013997:Time Factors
D006454:Hemoglobins; D004921:Erythropoietin; D005914:Globins; D005319:Fetal Hemoglobin; D007930:Leucine