A facile method for the preparation of N6-substituted ATP-Sepharose.
The attachment of 6-methylsulfonylpurineribose 5'-triphosphate to AH-Sepharose 4B as a facile method for the preparation of ATP-Sepharose is described. This reaction is compared to the coupling of 6-chloropurineriboside 5'-triphosphate as well as N(6)-aminocaproyl-ATP. An improved synthesis of purineriboside 5'-phosphates which does not require purification by column chromatography is also described.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives*; D001665:Binding Sites; D002846:Chromatography, Affinity / Q000379:methods*; D003488:Cyanogen Bromide; D011134:Polysaccharides; D012685:Sepharose
D011134:Polysaccharides; D000255:Adenosine Triphosphate; D012685:Sepharose; D003488:Cyanogen Bromide