Fluorometric screening method for detecting benzodiazepines in blood and urine.
We report a fluorometric method for detecting diazepam, chlordiazepoxide, oxazepam, chlorazepate, and (or) their major metabolites in blood, urine, or gastric contents at low therapeutic concentrations. The drugs are first hydrolyzed to their respective benzophenones and converted to highly fluorescent 9-acridanones. Total benzodiazepines (parent plus metabolites) in blood and gastric contents are semiquantitatively evaluated and compared to results of gas-chromatographic determinations.
D001569:Benzodiazepines / Q000032:analysis* / Q000097:blood / Q000652:urine; D002707:Chlordiazepoxide / Q000032:analysis; D003975:Diazepam / Q000097:blood; D005750:Gastric Juice / Q000032:analysis; D006801:Humans; D010076:Oxazepam / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D001569:Benzodiazepines; D010076:Oxazepam; D002707:Chlordiazepoxide; D003975:Diazepam