Inhibition of protein synthesis by D-threo-chloramphenicol in the laboratory and nodule forms of Rhizobium lupini.
Protein synthesis by both laboratory-grown bacteria and isolated nodule bacteroids of Rhizobium lupini (strain WU8) is inhibited by D-threo-chloramphenicol, the bacteroid form being the more sensitive to the antibiotic. A comparison between the two forms of the uptake of [14C]chloramphenicol showed that the bacteria always attained a lower intracellular chloramphenicol concentration. It is proposed that the sensitivity difference is due to a difference in membrane permeability between the two forms.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002463:Cell Membrane Permeability; D002701:Chloramphenicol / Q000494:pharmacology*; D004917:Erythromycin / Q000494:pharmacology; D008034:Lincomycin / Q000494:pharmacology; D010944:Plants / Q000382:microbiology*; D012231:Rhizobium / Q000187:drug effects / Q000378:metabolism*; D012988:Soil Microbiology
D001426:Bacterial Proteins; D004917:Erythromycin; D002701:Chloramphenicol; D008034:Lincomycin