The steady-state kinetics of peroxidase with 2,2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen.
The chemical nature of the important new chromogen ABTS [2,2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid)] is described together with an account of the redox and spectroscopic properties of the system ABTS--H2O2--peroxidase. Keq. is calculated and a study of the steady-state kinetics over a whole range of substrate concentrations is reported. By using novel methods of kinetic analysis, an interpretation of the results is given which requires some extension of the classical peroxidase mechanism.
D052160:Benzothiazoles; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D007700:Kinetics; D008433:Mathematics; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010544:Peroxidases; D013053:Spectrophotometry; D013451:Sulfonic Acids; D013844:Thiazoles
D052160:Benzothiazoles; D007202:Indicators and Reagents; D013451:Sulfonic Acids; D013844:Thiazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006861:Hydrogen Peroxide; D010544:Peroxidases