Simultaneous fluorescence histochemical demonstration of catecholamines and tryptophyl-peptides in endocrine cells.
Simple and efficient fluorescence histochemical methods for the concomitant demonstration of tryptophyl-peptide-containing cells and dopamine-containing cells have been developed in this study. Combined formaldehyde and chloral vapour or solution of 5% glyoxylic acid monohydrate in n-butanol induced concomitantly strong yellow fluorescence in the tryptophyl-peptide-containing cells and moderate green fluorescence in the dopamine-containing cells in the sections of the freeze-dried adenohypophysis.
D000085:Acetates / Q000494:pharmacology; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002697:Chloral Hydrate / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology; D005612:Freeze Drying; D006038:Glyoxylates / Q000494:pharmacology; D006651:Histocytochemistry; D007980:Levodopa / Q000494:pharmacology; D008297:Male; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism*; D014835:Volatilization
D000085:Acetates; D002395:Catecholamines; D006038:Glyoxylates; D010455:Peptides; D005557:Formaldehyde; D012701:Serotonin; D002697:Chloral Hydrate; D007980:Levodopa; D014364:Tryptophan; D004298:Dopamine