Specific role of magnesium ion in protecting the anticomplementary effect of DNA in immune hemolysis.
When a constant quantity of denatured DNA required for C-fixation reaction was employed as the antigen, an anticomplementary action of DNA was frequently observed. In order to cope with this difficulty in the analysis of C-fixation, the relationship between DNA, Mg2+ and C was investigated. It was found that this phenomenon was closely related to the flexible conformation of DNA which is highly influencd by Mg2+. Further, this effect could be eliminated in the presence of 5MM MgCl2. The mechanism of the elimination by Mg2+ of the anticomplementary effect of DNA was discussed.
D000818:Animals; D003168:Complement Fixation Tests; D004247:DNA / Q000032:analysis / Q000276:immunology*; D006461:Hemolysis / Q000187:drug effects*; D007106:Immune Sera; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D011817:Rabbits / Q000276:immunology; D012474:Salmon; D013094:Spermatozoa / Q000032:analysis
D007106:Immune Sera; D004247:DNA; D008274:Magnesium