A method for separating estrogens and phytoestrogens on Sephadex LH-20 columns.
A procedure for separating estrogens from phytoestrogens in animal fluids is presented. The use of Sephadex LH-20 in absolute methanol permits a simple separation and allows the measurement of phytoestrogen levels in plasma and urine by the competitive binding assay. This method facilitates a more accurate measure of the hormonal balance in animals with an exogenous input of phytoestrogens.
D002850:Chromatography, Gel / Q000379:methods; D004958:Estradiol / Q000097:blood / Q000302:isolation & purification; D004964:Estriol / Q000097:blood / Q000302:isolation & purification; D004967:Estrogens / Q000302:isolation & purification*; D004970:Estrone / Q000097:blood / Q000302:isolation & purification; D005419:Flavonoids / Q000302:isolation & purification*; D006801:Humans; D007529:Isoflavones / Q000302:isolation & purification*
D004967:Estrogens; D005419:Flavonoids; D007529:Isoflavones; D004970:Estrone; D004958:Estradiol; D004964:Estriol