Surgical procedure for intraamnionic inoculation of the bovine fetus.
A surgical procedure that ensures intraamnionic inoculation of the bovine fetus is described. Right flank surgical operation was performed, using a "grid"-type entry though the abdominal muscles. A portion of the uterus containing 1 fetal leg was presented for inoculating the test material between the hooves. The procedure was rapid and simple to do and was safe for both the dam and the fetus.
D000650:Amnion; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases; D005260:Female; D005315:Fetal Diseases / Q000662:veterinary; D005333:Fetus; D006192:Haemophilus Infections / Q000662:veterinary; D007267:Injections / Q000379:methods / Q000662:veterinary*; D007813:Laparotomy / Q000379:methods / Q000662:veterinary*; D011183:Postoperative Complications / Q000662:veterinary; D011247:Pregnancy; D014599:Uterus