Demonstration of lag phase in the sol-gel transformation of deoxygenated S hemoglobin without temperature alteration.
1). During the sol to gel transformation of deoxygenated sickle hemoglobin, a time-dependent process preceding gel formation (lag phase) was demonstrated that was inversely proportional to a function of the hemoglobin concentration and that occurred without alteration in temperature, pH, or oxygen tension. 2). As determined by the Schachman modification of the capillary viscometer, preparations of oxyhemoglobin S and A and deoxyhemoglobin A were indistinguishable when compared over a wide range of concentrations. Up to the concentration at which gelling occurred, deoxyhemoglobin S exhibited the same viscosity behavior. The viscosity of deoxygenated hemoglobin S within the lower gelling concentration range was normal during the lag phase and became abnormally high only at the time of gelation.
D000755:Anemia, Sickle Cell / Q000097:blood; D005319:Fetal Hemoglobin / Q000032:analysis; D006451:Hemoglobin, Sickle / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006720:Homozygote; D006801:Humans; D008706:Methemoglobin / Q000032:analysis; D012212:Rheology; D013435:Sulfhemoglobin / Q000032:analysis; D013696:Temperature; D013997:Time Factors; D014783:Viscosity
D006451:Hemoglobin, Sickle; D006455:Hemoglobins, Abnormal; D008706:Methemoglobin; D013435:Sulfhemoglobin; D005319:Fetal Hemoglobin