Studies on the persistence of differentiated functions in rat hepatocytes set into primary tissue cultures. Specific binding of 3H-bilirubin after eight days of staying in vitro.
Preliminary experiments were performed which indicate that even after 8 days of staying in primary in vitro tissue culture rat hepatocytes are still able to take up and bind tritiated, unconjugated bilirubin in a specific fashion and much more intensely than do connective tissue cells present in the same cultures. The data are suggestive of the maintenance in 8-day cultured hepatocytes of at least some of the specific pathways bound to bilirubin metabolism.
D000818:Animals; D001345:Autoradiography; D001663:Bilirubin / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured / Q000378:metabolism*; D046508:Culture Techniques; D005260:Female; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051381:Rats; D013997:Time Factors; D014316:Tritium
D014316:Tritium; D001663:Bilirubin