Estradiol-17beta biosynthesis in cultured granulosa cells from hypophysectomized immature rats; stimulation by follicle-stimulating hormone.
Granulosa cells isolated from the ovaries of hypophysectomized immature rats synthesize and secrete estradiol-17beta and estrone when grown for 2 days in monolayer culture in a synthetic medium containing testosterone (0.5 muM) and a highly purified follicle-stimulating hormone (FSH) preparation (0.25 mug/ml). Secretion is negligible in the absence of either testosterone or FSH, and a highly purified luteinizing hormone (LH) preparation (0.25 mug/ml) was without significant stimulatory effect. It is concluded that FSH regulates estrogen biosynthesis in granulosa cells of hypophysectomized rats by a specific stimulation of the aromatizing enzyme system.
D000818:Animals; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D007016:Hypophysectomy; D007986:Luteinizing Hormone / Q000494:pharmacology; D006080:Ovarian Follicle / Q000378:metabolism*; D010902:Pituitary Gland / Q000502:physiology*; D051381:Rats; D013739:Testosterone / Q000494:pharmacology
D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone