Sheep liver serine-threonine dehydratase. I. Purification and evidence for covalently linked alpha-ketobutyrate as its cofactor.
L-Serine-threonine dehydratase (EC 4.2.1.16) from sheep liver has been obtained as a highly purified preparation as shown by ultracentrifuge studies and analytical disc gel electrophoresis. The dehydratase has a molecular weight of 98,000 +/- 10,000 and is composed of two nonidentical subunits with molecular weights of 41,000 and 47,000. The 41,000 subunit is covalently linked to the carbonyl reagent-sensitive coenzyme which has been identified as alpha-ketobutyric acid.
D000818:Animals; D002087:Butyrates / Q000032:analysis*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003067:Coenzymes / Q000032:analysis*; D006836:Hydro-Lyases / Q000302:isolation & purification*; D007651:Keto Acids; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D012756:Sheep; D013913:Threonine Dehydratase / Q000032:analysis / Q000302:isolation & purification*
D002087:Butyrates; D003067:Coenzymes; D007651:Keto Acids; D046911:Macromolecular Substances; C005087:alpha-ketobutyric acid; D006836:Hydro-Lyases; D013913:Threonine Dehydratase