Structure of the O-glycosidically linked carbohydrate units of rat brain glycoproteins.
The O-glycosidically linked carbohydrate units of rat brain glycopeptides were released as reduced oligosaccharides with NaOH/NaBH4 treatment. Five oligosaccharides were isolated using gel filtration, ion-exchange chromatography and preparative thin-layer chromatography. Studies employing periodate oxidation, methylation analysis, chromium trixide oxidation and gas-liquid chromatography-mass spectrometry indicated the following structures: (I) alpha-galactosyl-(1 leads to 3)-N-acetylgalactosaminitol, (II) beta-galactosyl-(1 leads to 3)-N-acetylgalactosaminitol, (III) N-acetylneuraminyl-[beta-galactosyl-(1 leads to 3)-N-acetylgalactosaminitol], (IV) N-acetylneuraminyl-(2 leads to 3)-beta-galactosyl-(1 leads to 3)-N-acetylgalactosaminitol and (V) N-acetylneuraminyl-(2 leads to 3)-beta-galactosyl-(1 leads to 3)[N-acetylneuraminyl-(2 leads to 6)]-N-acetylgalactosaminitol.
D000818:Animals; D001894:Borohydrides; D001923:Brain Chemistry; D005260:Female; D005688:Galactosamine / Q000032:analysis; D005690:Galactose / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006027:Glycosides; D008297:Male; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats
D001894:Borohydrides; D006023:Glycoproteins; D006027:Glycosides; D009844:Oligosaccharides; D005688:Galactosamine; D005690:Galactose