Alterations in Sindbis viral enbelope proteins by treating BHK cells with glucosamine.
Addition of d-glucosamine to BHK cells infected with Sindbis virus inhibited the formation of the E-2 viral envelope from its precursor PE-2. Release of virus was blocked, and two new viral protein bands replaced the normal envelope protein bands detected in SDS-gel electropherograms of infected cell extracts.
D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine / Q000494:pharmacology*; D007668:Kidney; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D012845:Sindbis Virus / Q000276:immunology / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014764:Viral Proteins / Q000096:biosynthesis* / Q000276:immunology
D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D008715:Methionine; D005944:Glucosamine