Specific determination of testosterone in female plasma by radioimmunoassay: a rapid and reliable procedure for the routine clinical laboratory.
A rapid and reliable procedure for the specific determination of testosterone in female plasma is described. The assay entails a simple biphasic solvent partitioning of plasma extracts in order to remove cross-reacting steroids before quantitation of testosterone by radioimmunoassay using an antiserum raised against testosterone-11alpha-hemisuccinyl-bovine serum albumin. "Testosterone' values obtained in this way are in excellent agreeement with those obtained following thin-layer chromatographic purification of plasma extracts. However, the present procedure is easier, faster and cheaper to perform, and enables a single technician to assay 32 females plasma samples (in duplicate) in one working day.
D000311:Adrenal Glands / Q000502:physiology; D000315:Adrenalectomy; D000818:Animals; D002369:Castration; D002417:Cattle; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008722:Methods; D010053:Ovary / Q000502:physiology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine; D012997:Solvents; D013386:Succinates; D013739:Testosterone / Q000097:blood*; D013997:Time Factors
D012997:Solvents; D013386:Succinates; D012710:Serum Albumin, Bovine; D013739:Testosterone