On the activity of brain phospholipase A2 towards specifically labelled glycerophospholipids during subacute sclerosing panencephalitis.
1,2-Diacyl-sn-glycero-3-phosphorylcholine, -ethanolamine and -serine, specifically labelled with different fatty acids at either the 1- or 2-position, were prepared enzymatically using the acyltransferase system of rat liver microsomes. The substrates were subjected to hydrolysis by phospholipase A2 obtained from brain tissue of a normal and a case of subacute sclerosing panencephalitis (SSPE). In the pathological tissue and increase of approx. 50% in phospholipase A2 activity could be observed in comparison to that from the control brain for all investigated substrates. Experiments with phosphatidylethanolamines, specifically labelled with different fatty acids in the 2-position, revealed that the phospholipase A2 activity of the SSPE brain tissue was enhanced by about 50% when compared to the control brain regardless of the fatty acid constituent at the 2-position of the substrates.
D000293:Adolescent; D002540:Cerebral Cortex / Q000201:enzymology*; D006801:Humans; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D013344:Subacute Sclerosing Panencephalitis / Q000201:enzymology*; D013997:Time Factors
D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010718:Phosphatidylserines; D010743:Phospholipids; D010740:Phospholipases