Establishment of a cell line (SH-4) from pleural effusion of a patient with melanoma.
A continuous tissue culture line (SH-4) derived from pleural effusion cells of a patient with metastatic melanoma is described. The cells became established after five passages lasting a period of 5 months of slow growth. Doubling time of the continuous culture was 20 hr in passage 51. The cell line is now in passage 60. The cells of all passages examined appear spindle-shaped or oligodendritic as seen by light microscopy. Pigment deposition in the cells is light and incomplete, but more marked than in cells of the original pleural effusion as shown by electron microscopy. Chromosome complement shows a single peak with a modal number of 51.
D002455:Cell Division; D002460:Cell Line; D002875:Chromosomes / Q000032:analysis; D006801:Humans; D008545:Melanoma; D010996:Pleural Effusion