The difference in absorption of bull seminal ribonuclease by organ tissues of juvenile and adult mice.
Young (6- to 8-day-old) mice, weighing 3-5 g., died after the injection of 0.5 mg. bull seminal ribonuclease, whereas adult mice (24-27 g.) withstood a 10-fold amount given in one injection and a 30-fold amount administered in 15 injections without histological changes in the tissues or blood cell counts. The determination of the antitesticular efficiency of AS, AS RNase in the mouse revealed that kidney, heart, lung, peritoneal and spleen tissues in young animals significantly absorb in vitro the enzyme. In adult mice, absorption occurs only in spleen, testicular tissue and Crocker tumour tissue. The difference in absorption of AS RNase by the tissues of juvenile and adult mice was also confirmed by indirect immunofluorescence under conditions in vitro and even in vivo.
D000375:Aging; D000818:Animals; D002417:Cattle; D008297:Male; D051379:Mice; D012260:Ribonucleases / Q000378:metabolism*; D012510:Sarcoma 180 / Q000378:metabolism; D012661:Semen / Q000201:enzymology*
D012260:Ribonucleases