Studies on Triton X-100 detergent micelles.
Triton X-100 micelle formation at 25 degrees C was studied by use of sedimentation equilibrium and fluorescence spectroscopic techniques. The apparent molecular weight of the major Triton X-100 micelle was found to be 81250, indicating a micelle number of 125. A micelle number of 121 was obtained with fluorescence titration experiments, which showed one molecule of 1-anilino-8-naphthalene sulfonate binding per micelle with an apparent association constant of 0.9 x 10(5) M. The fluorescent titration experiments also indicated the presence of another TX-100 binding species of variable size.
D000817:Anilino Naphthalenesulfonates; D001665:Binding Sites; D003102:Colloids; D005453:Fluorescence; D007700:Kinetics; D008433:Mathematics; D008823:Micelles; D008970:Molecular Weight; D011092:Polyethylene Glycols; D000644:Quaternary Ammonium Compounds; D013050:Spectrometry, Fluorescence
D000817:Anilino Naphthalenesulfonates; D003102:Colloids; D008823:Micelles; D000644:Quaternary Ammonium Compounds; D011092:Polyethylene Glycols