The efficiency of 14C detection in autoradiographs of semithin plastic sections.
Sections of uniformly labeled 14C-methylmethacrylate were used to determine the efficiency of 14C detection by Ilford L4 emulsion layers, 0.22 mu in thickness, in light microscope autoradiographs. When efficiency was expressed as a function of radiation dose (i.e., the number of decays/mu2) there was a strong dependency on radiation dose up to about 6 decays/mu2, after which detection efficiency was fairly constant. For most emulsion batches, the detection efficiency was about 5%, but it ranged from 8 to 3%.
D001345:Autoradiography / Q000379:methods*; D002250:Carbon Radioisotopes; D004655:Emulsions; D005069:Evaluation Studies as Topic; D007553:Isotope Labeling; D008768:Methylmethacrylates / Q000032:analysis*
D002250:Carbon Radioisotopes; D004655:Emulsions; D008768:Methylmethacrylates