Binding of d-tubocurarine di (methyl-14C) ether iodide and other amines to cartilage, chondroitin sulfate and human plasma proteins.
The binding of d-tubocurarine di [methyl-14C] ether iodide (d-TCE) and other amines to bovine nasal septum, chondroitin sulfate (CS) and human plasma proteins was examined by equilibrium dialysis. Bovine nasal septum and CS bound d-TCE to a greater extent than they did morphine, d-methadone (d-ME) and l-methadone (l-ME). Cartilage, relative to an equal weight of pure CS, bound slightly more d-TCE, 50% more d-ME and l-ME, and the same amount of morphine. The amount of d-TCE bound to pure CS was similar to that of decamethonium [methyl-14C] dibromide but less than that of two new iodinated bisquaternary compounds: 1,6-bis(N,N-dimethyl-3-iodobenzylamino)hexane dichloride and 1,6-bis[dimethyl-(3-amino-4,6-diiodo-benzyl)amino]hexane dichloride. The binding of d-TCE is greater for pure CS than for impure CS which contains 26% protein, and binding to either preparation was inversely related to sodium ion concentration. In the drug concentration range explored, 30 to 40% of d-TCE is bound to plasma proteins, which is twice that of decamethonium binding but equivalent to the binding of morphine and about one-half the binding of d-ME and l-ME. In this same drug concentration range, each gram of cartilage (dry weight) can bind about 10(-7) mol of d-TCE. This study suggests that the binding of d-TCE to cartilage is an ionic type bonding to the anionic sites of the ester sulfates and glucuronate moieties of CS and that the binding of d-TCE to the protein fraction of cartilage is probably equal to, or less than, its binding to the CS fraction. It is concluded that cartilage may represent a measurable distribution pool for d-tubocurarine.
D000588:Amines / Q000378:metabolism*; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002356:Cartilage / Q000378:metabolism*; D002417:Cattle; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008691:Methadone / Q000378:metabolism; D009020:Morphine / Q000378:metabolism; D009300:Nasal Septum / Q000378:metabolism; D011485:Protein Binding; D014403:Tubocurarine / Q000031:analogs & derivatives* / Q000378:metabolism
D000588:Amines; D001798:Blood Proteins; D009020:Morphine; D002807:Chondroitin; D002809:Chondroitin Sulfates; D008691:Methadone; D014403:Tubocurarine