A new approach to the study of phospholipid-protein interactions in biological membranes. Synthesis of fatty acids and phospholipids containing photosensitive groups.
In a general approach to the study, in vivo and in vitro, of the interactions between phospholipids and proteins in biological membranes, a variety of fatty acids containing photosensitive groups in different positions in the alkyl chains has been synthesized. The fatty acids synthesized include: 16-azidopalmitelaidic acid, 12-azidooleic acid, 6-, 9-, 11-, and 12-azidostearic acid, 12-omicron-(ethyl-2-diazomalonyl)stearic and -oleic acids, 12-omicron-(4-azido-2-nitrophenyl)stearic and -oleic acids, and 12-oxo-10-octadecenoic acid. Some of the above synthetic fatty acids were also prepared in the radioactively labeled form. For in vitro studies, many of the above fatty acids were used to acylate the 2 position in the preparation of a number of mixed acylphosphatidylcholines and mixed acylphosphatidylethanolamines. On sonication, the synthetic phospholipids formed sealed vesicles. Intermolecular cross-linking of the fatty acyl chains in phospholipids was demonstrated on photolysis of the vesicles.
D005227:Fatty Acids / Q000138:chemical synthesis*; D008027:Light; D008081:Liposomes; D008567:Membranes, Artificial; D008954:Models, Biological; D009829:Oleic Acids / Q000138:chemical synthesis; D010743:Phospholipids / Q000138:chemical synthesis; D010782:Photolysis; D011506:Proteins; D013229:Stearic Acids / Q000138:chemical synthesis; D013329:Structure-Activity Relationship
D005227:Fatty Acids; D008081:Liposomes; D008567:Membranes, Artificial; D009829:Oleic Acids; D010743:Phospholipids; D011506:Proteins; D013229:Stearic Acids