Polyadenylation of RNA in vitro in isolated chromatin and nuclei.
Poly(A) is added post-transcriptionally to RNA transcribed in vitro by endogenous form B DNA-dependent RNA polymerase bound to the template in isolated nuclei or chromatin. It is also added to processed fragments of the products from alpha-amanitin-resistant RNA polymerases A and/or C. The poly(A) segments are of similar size to those found in nuclear RNA pulse-labelled in vivo and are added onto the 3' terminus of RNA chains (whether pre-existing, completed during the incubation in vitro or created by fragmentation of larger RNA transcripts). That poly(A) addition is not directly mediated by any of the nuclear DNA-dependent RNA polymerases is shown by the differential sensitivities of RNA and poly(A) syntheses to increasing ionic strength and transcriptional inhibitors such as the exotoxin from Bacillus thuringiensis.
D000818:Animals; D001483:Base Sequence; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D002846:Chromatography, Affinity; D008099:Liver / Q000378:metabolism; D008970:Molecular Weight; D011061:Poly A / Q000096:biosynthesis / Q000302:isolation & purification; D012313:RNA / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D013698:Templates, Genetic
D002843:Chromatin; D011061:Poly A; D012313:RNA