Effects of SH-reagents of different molecular size upon glucose metabolism in isolated rat fat cells.
To study the role of membrane SH-groups in glucose transport of isolated rat fat cells we compared the effects of a small organic mercurial reagent p-CMB with those of a large p--CMB-derivative -- p-CMB-Dextran, MW  10.000 --. It could be shown that both compounds were of almost identical reactivity on fat cell homogenate metabolism. When applied to intact fat cells uncoupled p--CMB showed an (1) insulin like enhancement of 14C incorporation from (U-14C) glucose into CO2 and triglyceride, (2) inhibition of the insulin-stimulatory effect on these parameters and (3) inhibition of basal glucose uptake dependent on the concentrations used. Identical concentrations of p-CMB-Dextran, however, failed to influence basal glucose uptake as well as the insulin mediated increase in glucose metabolism.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002729:Chloromercuribenzoates / Q000494:pharmacology*; D003911:Dextrans / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008970:Molecular Weight; D051381:Rats; D014280:Triglycerides / Q000096:biosynthesis
D002729:Chloromercuribenzoates; D003911:Dextrans; D014280:Triglycerides; D002245:Carbon Dioxide; D005947:Glucose