Accessibility of the minor groove of DNA in chromatin to the binding of antibiotics netropsin and distamycin A.
The interaction of the antibiotics distamycin A, distamycin analogue and netropsin with chromatin of calf thymus has been studied by circular dichroism measurements and by gel filtration. The minor groove of DNA in chromatin is accessible by 83-89% to the binding of these antibiotics as compared with that of free DNA. The present results combined with our data on the methylation of chromatin with dimethylsulphate [3] strongly suggest that the minor groove of DNA in chromatin is not occupied by chromatin proteins.
D000818:Animals; D000900:Anti-Bacterial Agents; D001665:Binding Sites; D002417:Cattle; D002843:Chromatin / Q000032:analysis / Q000648:ultrastructure*; D002850:Chromatography, Gel; D002942:Circular Dichroism; D004247:DNA / Q000032:analysis; D004214:Distamycins; D006146:Guanidines; D009690:Nucleic Acid Conformation; D011758:Pyrroles; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013950:Thymus Gland
D000900:Anti-Bacterial Agents; D002843:Chromatin; D004214:Distamycins; D006146:Guanidines; D011758:Pyrroles; D004247:DNA