[Effect of naproxen on DNA synthesis and DNA repair of human lymphocytes in in vivo experiments].
This investigation was conducted in order to evaluate the possibility of late defects caused by drugs. After their base-line blood levels had been assessed, 3 patients with classical rheumatoid arthritis (according to ARA criteria; American Rheumatism Association) received 500 mg of d-2-(6'-methoxy-2'-naphthyl)-propionic acid (naproxen) daily (in capsules). At the peak of naproxen blood levels following the first naproxen dose, and once monthly for 7 months, thereafter, blood samples were drawn. After separating the lymphocytes from the whole blood, they were damaged by means of 60-Co. Hydroxyurea was added for the suppression of their semiconservative DNA-synthesis. The incorporation of 3-H-THYMIDINE INTO THE DNA of the cells was measured after radiation at different incubation periods. The results showed normal behaviour of the DNA-synthesis following naproxen administration in all three patients. The DNA-repair capacity was normal in one female patient, but slightly lowered in the remaining two.
D001172:Arthritis, Rheumatoid / Q000097:blood; D002908:Chronic Disease; D003037:Cobalt Radioisotopes; D004247:DNA / Q000096:biosynthesis*; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D008875:Middle Aged; D009280:Naphthaleneacetic Acids / Q000494:pharmacology*; D009288:Naproxen / Q000494:pharmacology*; D011239:Prednisolone / Q000494:pharmacology; D011830:Radiation Effects; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D003037:Cobalt Radioisotopes; D009280:Naphthaleneacetic Acids; D014316:Tritium; D009288:Naproxen; D004247:DNA; D011239:Prednisolone; D013936:Thymidine; D006918:Hydroxyurea
Uber den Einfluss von Naproxen auf die DNS-Synthese und DNS-Reparatur menschlicher Lymphozyten im In-vivo-Versuch