Identification of microorganisms by gas chromatographic-mass spectrometric analysis of cellular fatty acids.
The cellular fatty acid compositions of strains of Pseudomonas aeruginosa, P. cepacia, and P. maltophilia were determined by gas-liquid chromatography. A variety of acids was detected in these organisms, including branched and straight-chain acids, cyclopropane, and hydroxy acids. Comparison of the presence and relative amounts of these acids was useful in distinguishing the three species. Three branched-chain hydroxy acids not found in other bacteria were present in P. maltophilia and were identified by gas chromatography, mass spectrometry, and infrared spectroscopy.
D002473:Cell Wall / Q000032:analysis; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D005233:Fatty Alcohols / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D011549:Pseudomonas / Q000032:analysis* / Q000145:classification; D013055:Spectrophotometry, Infrared
D005227:Fatty Acids; D005233:Fatty Alcohols