Intestinal glycoproteins of germfree rats. IV. Oligosaccharides obtained by chemical degradation of a water-soluble glycoprotein fraction.
Removal of sialic acid from the glycoprotein and subsequent degradation with sodium hydroxide-sodium borohydride resulted in the formation of a number of low-molecular weight carbohydrate compounds of which three were isolated and characterized: 2-deoxy-2-acetamido-D-galactitol; O-alpha-6-fucopyranosyl (1 leads to 2)-O-Beta-D-galactopyranosyl-(1 leads to 3)-2-deoxy-2-acetamido-D-galactitol (oligosaccharide I); O-Beta-D galactopyranosyl-(1 leads to 3)-2-deoxy-2-acetamido-D-galactitol (oligosaccharide II). From an acetolysate of the desialized glycoprotein a fourth component was obtained and characterized to be O-Beta-D-galactopyranosyl-(1 leads to 4)-2-deoxy-2-acetamido-D-glucose (oligosaccharide III).
D000818:Animals; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D004848:Epithelium / Q000032:analysis; D005856:Germ-Free Life; D006023:Glycoproteins / Q000032:analysis*; D007413:Intestinal Mucosa / Q000032:analysis; D007422:Intestines / Q000032:analysis*; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats
D006023:Glycoproteins; D009844:Oligosaccharides