In vitro influence of steroidal hormones on protein and glucose metabolism of isolated granulomatous tissue cells.
A standard method of preparing isolated cells from granulomatous tissue capsules was developed. Results of morphological and chemical studies indicated that the cells were intact and functional. An inhibitory effect of glucocorticoids added in vitro was demonstrated for the first time, and this effect was consonant with the influence of glucocorticoids on granulomas in vivo. The isolated cells were predominantly monocytes, macrophages and lymphocytes with smaller numbers of polymorphonuclear leucocytes, mast cells and fibroblasts. Dimethyl sulfoxide, used to aid steroid solubilization (0.0016 ml/ml), had no influence on protein synthesis or on glucose oxidation. Cholesterol, a nonhormonally active sterol (1 x 10(-4) M, 3 x 10(-4) M), had no effect on labeled glycine incorporation. Methylprednisolone (1 x 10(-4) M, 3 x 10(-4) M) decreased protein synthesis after 30 min of incubation indicating that this might be one important early mechanism of action. Methylprednisolone (1 x 10(-4) M) had no influence on glucose oxidation. Deoxycorticosterone (1 x 10(-4) M, 3 X 10(-4) M) inhibited both protein synthesis and glucose oxidation after 30 min of incubation.
D000818:Animals; D002784:Cholesterol / Q000494:pharmacology; D003900:Desoxycorticosterone / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D006099:Granuloma / Q000378:metabolism* / Q000473:pathology; D007962:Leukocytes / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology*; D009000:Monocytes / Q000378:metabolism; D010084:Oxidation-Reduction; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
D011506:Proteins; D003900:Desoxycorticosterone; D002784:Cholesterol; D005947:Glucose; D005998:Glycine; D008775:Methylprednisolone; D004121:Dimethyl Sulfoxide