Distribution of Corynebacterium renale among healthy bulls with special reference to inhabitation of type III in the prepuce.
Distribution of Corynebacterium renale among apparently healthy bulls reared in Hokkaido was investigated. The organism was detected from 46 (39.3%) of 117 specimens of preputial cavity washing and from 60 (51.7%) of 116 specimens of semen. The isolates studied in this survey belonged to type III, except a few which belonged to type II. No type I strain was isolated from any bull. C. renale type III was isolated from the prepuce in six of seven bulls slaughtered and from urethra in three, but not at all from any other organ. In the seven bulls, no macroscopic changes were seen, but a slight infiltration of lymphocytes and formation of lymph nodules were noticed in the prepuce. No other microscopical changes could be demonstrated in any other organ. No serum antibody response was detected. To ascertain the virulence of C. renale isolated from the bulls, a strain of type II was inoculated into the urinary bladder of a healthy cow. The cow exhibited fever and hematuria on and after the 10th day. Typical cystitis was proved when the cow was necropsied on the 14th day after inoculation. From these result it is conceived that C. renale type II organisms inhabit the prepuce of apparently healthy bulls at a high rate, without inducing any disturbance.
D000818:Animals; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000473:pathology; D003352:Corynebacterium / Q000302:isolation & purification* / Q000472:pathogenicity; D003354:Corynebacterium Infections / Q000150:complications / Q000473:pathology / Q000662:veterinary; D003556:Cystitis / Q000209:etiology / Q000473:pathology / Q000662:veterinary; D005260:Female; D008297:Male; D010413:Penis / Q000382:microbiology*; D012661:Semen / Q000382:microbiology; D014521:Urethra / Q000382:microbiology; D001743:Urinary Bladder / Q000473:pathology; D014774:Virulence