The synthesis of mono- and bifunctional nitriles and imidoesters carrying a fluorescent group.
The synthesis of nitriles and dinitriles carrying fluorescent dansyl groups is described. From these compounds the corresponding mono- and bifunctional imidoesters were prepared. They are suitable for labelling or crosslinking, respectively, of proteins under mild conditions.
D001665:Binding Sites; D003619:Dansyl Compounds; D004952:Esters; D005453:Fluorescence; D005969:Glutamate Dehydrogenase / Q000032:analysis; D007094:Imides / Q000138:chemical synthesis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008722:Methods; D009570:Nitriles / Q000138:chemical synthesis*; D011485:Protein Binding; D013329:Structure-Activity Relationship
D003619:Dansyl Compounds; D004952:Esters; D007094:Imides; D009570:Nitriles; D007770:L-Lactate Dehydrogenase; D005969:Glutamate Dehydrogenase