Fractionation of plant aminoacyl-tRNA synthetases on tRNA-Sepharose columns.
It has been shown that tRNA-Sepharose, a chromatographic adsorbent containing unfractionated tRNA bound to a Sepharose matrix, is a useful, group-specific adsorbent for fractionation of the plant aminoacyl-tRNA synthetases. Conditions are described in which Val-, Trp-, Phe-, Leu- and Ile-tRNA synthetases from yellow lupin seeds can be separated from each other on the tRNA-Sepharose columns. Factors affecting affinity chromatography on the t-RNA-Sepharose columns are discussed. The affinity chromatography procedure for the purification of lupin Ser-tRNA synthetase to homogenity is described.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D007533:Isoleucine-tRNA Ligase / Q000302:isolation & purification; D007935:Leucine-tRNA Ligase / Q000302:isolation & purification; D010652:Phenylalanine-tRNA Ligase / Q000302:isolation & purification; D010944:Plants / Q000201:enzymology*; D012343:RNA, Transfer; D012685:Sepharose; D014369:Tryptophan-tRNA Ligase / Q000302:isolation & purification; D014637:Valine-tRNA Ligase / Q000302:isolation & purification
D012685:Sepharose; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D014369:Tryptophan-tRNA Ligase; D010652:Phenylalanine-tRNA Ligase; D007935:Leucine-tRNA Ligase; D007533:Isoleucine-tRNA Ligase; D014637:Valine-tRNA Ligase