Radioimmunoassay of 17-hydroxyprogesterone in serum with or without thin-layer chromatographic purification.
A radioimmunoassay for the measurement of 17-hydroxyprogesterone (17-OHP) is described. The antigen 11-desoxycortisol-21-hemisuccinate-bovine serum albumin has been used to produce two antisera of different antibody populations in the same animal. The thin-layer chromatographic system described can be used to separate all the cross-reacting steroids investigated from 17-OHP. A simplified method is also presented using preliminary solvent extraction only. The mean 17-OHP levels measured, using this method, were, for normal men, 0.86 +/- 0.19 ng/ml (SD), for normal females 0.30 +/- 0.19 ng/ml in the follicular phase and 1.72 +/- 0.18 ng/ml in the luteal phase of the menstrual cycle, and 0.45 +/- 0.17 ng/ml in normal postmenopausal women.
D002855:Chromatography, Thin Layer; D005069:Evaluation Studies as Topic; D005260:Female; D005498:Follicular Phase; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood* / Q000302:isolation & purification; D008183:Luteal Phase; D008297:Male; D008593:Menopause; D011863:Radioimmunoassay / Q000379:methods
D006908:Hydroxyprogesterones