Separation of the coprophorphyrin isomers I and III by thin-layer chromatography.
An improved, specific and sensitive method for the determination of the coproporphyrin isomers I and III is described. In this method, the hydrolysis of the coproporphyrin methyl esters takes place in the silica gel layer of acid-resistant pre-coated thin layer plates. After chromatography in the solvent system 2,6-dimethylpyridine/water, (volume ratio 20 ml + 6 ml), in an ammonia atmosphere, the isomers are measured, either directly in the silica gel layer by fluorimetry, or after elution in 1.0 mol/l hydrochloric acid. The technique makes analysis in the nanogram range possible. See article.
D002855:Chromatography, Thin Layer; D003306:Coproporphyrins / Q000652:urine*; D006801:Humans; D006868:Hydrolysis; D007536:Isomerism; D007855:Lead Poisoning / Q000652:urine; D008722:Methods; D011164:Porphyrias / Q000652:urine; D011166:Porphyrins / Q000652:urine*; D012997:Solvents; D013053:Spectrophotometry
D003306:Coproporphyrins; D011166:Porphyrins; D012997:Solvents