[Method for the primary selection of active antitumor alkylating preparations in vitro and in vivo].
To reveal the cytotoxic activity of antitumor drugs the experiments were conducted in vitro-in vivo. Active substances in the dosage of 800 mg per 1 Kg of tumor were found to suppress sarcoma 45 transplantability during 1 hour in vitro. But inactive substances did it only in much larger doses. The result of the in vivo-in vitro experiments coincided with the results obtained by other investigators studying the antitumor activity in vivo. The experiments using the proposed method of selection required a much less amount of the substance (a biological experiment, repeated twice, can be carried out having 1--3 mg), animals, time and efforts of the researcher.
D000477:Alkylating Agents / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D066298:In Vitro Techniques; D007928:Lethal Dose 50; D009368:Neoplasm Transplantation; D051381:Rats; D012513:Sarcoma, Experimental / Q000188:drug therapy; D013997:Time Factors
D000477:Alkylating Agents; D000970:Antineoplastic Agents
Sposob pervichnogo otbora aktivnykh protivoopukholevykh alkiliruiushchikh preparatov in vitro--in vivo