Immunoferritin and immune electron microscopic study of bovine herpesvirus strain DN-599.
Intracellular antigens of strain DN-599 bovine herpesvirus were detected in the cytoplasm and the nucleus of infected bovine embryonic kidney cells by the indirect immunoferritin (IF) technique. Specific tagging was observed in viral envelope and capsids. Aggregates of viral particles heavily coated with antibody were seen by immune electron microscopy (IEM).
D000818:Animals; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005293:Ferritins; D006041:Goats / Q000276:immunology; D006564:Herpesviridae / Q000254:growth & development / Q000276:immunology / Q000648:ultrastructure*; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure; D007668:Kidney; D008854:Microscopy, Electron; D011817:Rabbits / Q000276:immunology; D014461:Ultracentrifugation
D000956:Antigens, Viral; D005293:Ferritins