Replication of eastern equine encephalitis viruses (New Jersey and Louisiana strains and the Ets-4 mutant) in rabbit kidney cells.
Virus yield, viral RNA synthesis, viral protein synthesis, cytopathology, and virus-induced shutoff of host protein synthesis were examined in rabbit kidney (RK) cells infected with eastern equine encephalitis (EEE) viruses. The New Jersey (NJ) strain replicated most rapidly, and exhibited a distinct patter in the synthesis of structural and non-structural viral proteins. The Louisiana (LA) strain and the Ets-4 mutant appeared to be similar in RK celles and chick embryo fibroblasts. Although the viral peptides comprising the viral RNA polymerase could not be conclusively identified, two non-structural proteins of molecular weights 105,000 and 85,000 were temporally associated with increasing rates of polymerase activity in NJ-infected RK cells and were found in increased amounts in Ets-4-infected cells compared to La-infected cells.
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D004663:Encephalitis Virus, Eastern Equine / Q000254:growth & development* / Q000378:metabolism; D004668:Encephalitis Viruses / Q000254:growth & development*; D007668:Kidney; D009154:Mutation; D010455:Peptides / Q000032:analysis; D012367:RNA, Viral / Q000096:biosynthesis; D011817:Rabbits; D013696:Temperature; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins