Purification of Japanese encephalitis virus vaccine by zonal centrifugation.
A large-scale procedure for purification and concentration of Japanese encephalitis virus vaccine by a continuous-flow isopycnic banding technique in a sucrose density gradient solution, using a K-III zonal centrifuge rotor, is presented. The quality of zonal-purified vaccine was compared with commercial and Japanese National Institutes of Health reference vaccines for antigenicity, immunodiffusion, and allergic encephalitogenicity tests to show its high purity.
D000818:Animals; D002501:Centrifugation, Zonal; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology; D006168:Guinea Pigs; D051379:Mice; D011817:Rabbits; D013395:Sucrose; D014765:Viral Vaccines / Q000302:isolation & purification* / Q000592:standards
D014765:Viral Vaccines; D013395:Sucrose