An improved method for the determination of 5-phosphoribosyl 1-pyrophosphate.
A spectrophotometric method for the determination of 5-phosphoribosyl 1-pyrophosphate (PRPP) is presented which shows several advantages in comparison to the radiochemical techniques, such as a relatively simple, rapid and less expensive procedure. This technique has been used to evaluate PRPP content in erythrocytes, leukocytes and lymphocytes of normal subjects and individuals with partial hypoxanthine guanine phosphoribosyltransferase (EC 2.4.2.8) deficiency. The results obtained proved to be completely reliable in both groups of subjects examined, with values of PRPP similar to those observed by radiochemical techniques.
D000328:Adult; D000818:Animals; D001773:Blood Cells / Q000032:analysis*; D002417:Cattle; D004912:Erythrocytes / Q000032:analysis; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency*; D007962:Leukocytes / Q000032:analysis; D008099:Liver / Q000032:analysis; D008214:Lymphocytes / Q000032:analysis; D010428:Pentosephosphates / Q000097:blood*; D010754:Phosphoribosyl Pyrophosphate / Q000097:blood*; D051381:Rats; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D014527:Uric Acid / Q000097:blood
D010428:Pentosephosphates; D014527:Uric Acid; D010754:Phosphoribosyl Pyrophosphate; D007041:Hypoxanthine Phosphoribosyltransferase