Insulin-unresponsive tissues respond to superactive insulin-like material.
Insulin-like material prepared from insulin-Sepharose stimulates glucose oxidation by isolated diaphragm of C57Bl/6J ob/ob mice, but insulin does not. This material is much more effective than insulin on epididymal fat tissue from these mice. Insulin-like material and insulin are equipotent on the corresponding tissues from lean littermates.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D003964:Diaphragm / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D007333:Insulin Resistance; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese
D002250:Carbon Radioisotopes; D007328:Insulin; D005947:Glucose