[The rapid determination of urinary estriol in pregnancy by thin layer chromatography (author's transl)].
A simplified method for the determination of urinary estriol in pregnancy by thin layer chromatography is described. After precipitation of the conjugated substances with ammonium sulfate a short hydrolysis is carried out. Estriol is extracted with diethylether and coupled with dark blue R salt to an azo-dye. After thin layer chromatographic isolation, a spectrophotometric determination from the chromatogram is done. The recovery rate is 94%. The mean error in serial determination is +/- 5,4%. 1 nanogram (10(-9) g) of estriol can be determined from the thin layer chromatogram. The time required to obtain a result is just over 1 hour.
D002855:Chromatography, Thin Layer / Q000379:methods; D004964:Estriol / Q000652:urine*; D005260:Female; D006801:Humans; D006868:Hydrolysis; D011247:Pregnancy
D004964:Estriol
Zur dünnschichtchromatographischen Schnell-bestimmung von Ostriol aus Harn von Schwangeren