A rapid quantitative determination of triglycerides by means of thin-layer chromatography.
A quantitative chromatographic method for determining triglycerides in serum is described. The serum is applied directly to a thin-layer plate and after partition with one developing solvent the fractions are charred with 10% sulphuric acid. Charring takes 20 minutes. The concentration of triglycerides is measured against a triolein standard, which in itself is corrected for having too many double bonds by scanning the charring of the triglyceride fraction with a "flying spot"-scanner or a slitscanner. The results are compared with other methods of determination.
D002855:Chromatography, Thin Layer / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D013997:Time Factors; D014280:Triglycerides / Q000097:blood*
D014280:Triglycerides