Preparation of high-quality iodine-125-labelled pituitary luteinizing hormone for radioimmunoassay.
High quality pituitary luteinizing hormone labelled with 125-I was obtained after separating out the more heavily iodinated fractions, through starch gel electrophoresis, using the cathodal component (Fraction 1) which was further purified on Sephadex G-100, giving an almost pure 125-I-labelled luteinizing hormone preparation, presenting excellent immunoreactivity and low levels of damage on incubation in plasma. The quality control of the steps of the technique was done with plasma-coated talc (200 mg) which compared favorably, as far as being an indication of intact labelled luteinizing hormone, with the more time-consuming chromatoelectrophoresis.
D000818:Animals; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D004592:Electrophoresis, Starch Gel; D007457:Iodine Radioisotopes; D007986:Luteinizing Hormone / Q000032:analysis* / Q000302:isolation & purification; D008722:Methods; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013627:Talc
D007457:Iodine Radioisotopes; D013627:Talc; D007986:Luteinizing Hormone