The susceptibility of calves to infection with a strain of bovine ephemeral fever virus inoculated intracerebrally.
Three newborn calves were inoculated intracerebrally with bovine ephemeral fever virus strain 525. The 2 that lacked detectable neutralising antibody to bovine ephemeral fever vaccine developed fatal encephalitis after 4 and 7 days respectively. The third calf which had a low level of maternal antibody remained healthy and developed antibody that became undetectable after 6 months. Bovine ephemeral fever virus strain 525 was reisolated from the brains of both dead calves by intracerebral inoculation of suckling mice. Homogenates that were prepared from the brains of the calves failed to produce disease or to induce antibody formation in susceptible calves when inoculated intravenously. Strain 525 of BEF virus has been shown to possess a degree of neurovirulence for laboratory animals that has not been reported for other strains (Tzipori and Spradbrow 1974). Although this strain is unable to produce viraemia in calves after I/V inoculation, the present study shows that strain 525 can multiply in the brain tissues of calves and cause death after I/C inoculation.
D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000096:biosynthesis; D001921:Brain / Q000382:microbiology; D002417:Cattle; D004660:Encephalitis / Q000209:etiology / Q000662:veterinary; D004810:Ephemeral Fever / Q000276:immunology / Q000382:microbiology*; D007267:Injections; D051379:Mice; D012328:RNA Viruses / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*
D000914:Antibodies, Viral