A high molecular weight RNA fraction in chromatin.
Utilizing a new chromatin isolation and fractionation technique we have obtained a high molecular weight RNA fraction from L-929 cell chromatin. The synthesis of this RNA is not greatly inhibited by concentrations of 0.04 mug/ml actinomycin D in the medium. Its synthesis appears to be strongly inhibited by 2 mug/ml of alpha-amanitin. The RNA appears to be quickly degraded (or removed from the chromatin) and does not contain a poly(A) sequence at its 3'-OH terminal end. Our working hypothesis is that this RNA is "nascent" heterogenous nuclear RNA partially transcribed from regions of the chromatin.
D000227:Adenine Nucleotides / Q000032:analysis; D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D007739:L Cells / Q000378:metabolism; D008793:Metrizamide / Q000494:pharmacology; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D011485:Protein Binding; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000302:isolation & purification; D012343:RNA, Transfer / Q000302:isolation & purification; D014529:Uridine / Q000378:metabolism
D000227:Adenine Nucleotides; D000546:Amanitins; D002843:Chromatin; D012335:RNA, Ribosomal; D003609:Dactinomycin; D012313:RNA; D012343:RNA, Transfer; D008793:Metrizamide; D014529:Uridine