[Repair replication in synchronous Ehrlich ascites cells after reversal of a methotrexate block (author's transl)].
If synchronous Ehrlich ascites cells are arrested at the beginning of the S phase by a methotrexate block, and the block is reversed by addition of thymidine to the culture, the cells incorporate a considerable amount of this thymidine into parental DNA within the first minute. These newly synthesized segments are irregularly distributed among the total parental DNA. This kind of distribution is expected if these segments are the result of excision repair. On the basis of the kinetics of the process this repair probably is DNA synthesis in regions already excised during the methotrexate block.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002455:Cell Division; D002499:Centrifugation, Density Gradient; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D008727:Methotrexate / Q000494:pharmacology*; D051379:Mice; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D013936:Thymidine; D008727:Methotrexate
Reparatur-Replikation in synchronen Ehrlich-Asciteszellen nach Aufhebung eines Methotrexat-Blocks