Poly (A) polymerase of bovine lymphosarcoma.
Poly(A) polymerase has been extensively purified from low-salt extracts of bovine lymphosarcoma. The enzyme is Mn2+ dependent, requires an oligonucleotide or RNA primer, incorporates only adenosine triphosphate, and is inhibited by other ribonucleotides or deoxynucleotides. Oligoadenylate and ribosomal RNA are good primers for the enzyme; transfer RNA and poly(A) are poor. RNA transcribed in vitro by homologous RNA polymerase is an efficient primer. The properties of the enzyme are similar to the properties of the Mn2+ -activated poly(A) polymerase of calf thymus. Approximately the same amount of enzyme appears to be present in lymphosarcoma and calf thymus.
D000227:Adenine Nucleotides; D000255:Adenosine Triphosphate; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000201:enzymology*; D055598:Chemical Phenomena; D002621:Chemistry; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors; D003854:Deoxyribonucleotides; D008228:Lymphoma, Non-Hodgkin / Q000201:enzymology* / Q000662:veterinary; D008345:Manganese; D009713:Nucleotidyltransferases / Q000302:isolation & purification*; D009841:Oligonucleotides; D011119:Polynucleotides; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D012343:RNA, Transfer; D012265:Ribonucleotides; D013950:Thymus Gland / Q000201:enzymology
D000227:Adenine Nucleotides; D003854:Deoxyribonucleotides; D009841:Oligonucleotides; D011119:Polynucleotides; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D012265:Ribonucleotides; D008345:Manganese; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D009713:Nucleotidyltransferases; D012321:DNA-Directed RNA Polymerases