The synthesis of Forssman glycolipid in clones of nil 2 hamsters fibroblasts grown in monolayer or spinner culture.
The amount of Forssman glycolipid (GL-5) was investigated in two clones of Nil hamster cells grown either in monolayer or in spinner culture. GL-5 assayed by the complement-fixation inhibition test increased with increasing cell density in the monolayer. However, cells grown in spinner cultures failed to show the density-dependent response in both clones examined. Cells transformed by hamster sarcoma virus did not show the density-dependent increase of GL-5, even in cells grown in monolayer. The effect of transfer from confluent to sparse cultures on the amount and the synthesis of GL-5 was also examined. It is suggested that the GL-5 that accumulates in cells during confluency is diluted into the daughter cells and that the decrease of the Forssman lipid does not precede cell division.
D000818:Animals; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002554:Cerebrosides / Q000096:biosynthesis*; D002999:Clone Cells; D003168:Complement Fixation Tests; D006224:Cricetinae / Q000382:microbiology; D046508:Culture Techniques / Q000379:methods*; D005347:Fibroblasts / Q000378:metabolism*; D005915:Globosides; D012513:Sarcoma, Experimental / Q000382:microbiology
D002554:Cerebrosides; D005915:Globosides; C010163:Forssman glycolipid