Collaborative study of the glass wool filtration method for the recovery of virus inoculated into ground beef.
A method for estimating viral population levels in ground beef was studied collaboratively in 7 laboratories. The collaborators recovered virus from 6 inoculated samples. Three samples were replicates of the high virus concentration 050 plaque-forming units (pfu)/g) and 3 replicates represented the low concentration (10 pfu/g). Six of the 7 collaborators recovered acceptable levels of virus from the samples. The per cent of variation was 30.6 for the high concentration and 18.5 for the low concentration. Collaborators did not differ from one another significantly in the results obtained for the 10 pfu/g samples, but results from one collaborator were significantly low for the recovery of virus from the 50 pfu/g samples. The results indicate that the glass wool filtration method is adequate for the detection of a number of viruses that may be found in foods. The method has been adopted as official first action.
D000818:Animals; D005374:Filtration; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005898:Glass; D008460:Meat / Q000032:analysis*; D014780:Viruses / Q000302:isolation & purification*; D014935:Wool