Quantitation of the biosynthesis of immunoglobulin in peripheral blood lymphocytes of normal and immunodeficient patients.
A solid phase radioimmunoassay was applied to the study of biosynthesis of immunoglobulins in lymphocytes cultured from peripheral blood of normal and immunodeficient patients. Total immunoglobulin and IgG were readily detected in lymphocyte culture fluids in studies of all normal individuals (age range 28 weeks, gestation to adulthood). In contrast, synthesis of immunoglobulins was absent or markedly reduced in cultures from patients with humoral immunodeficiencies. Inasmuch as this method requires relatively small amounts of blood, it may be of particular value in the diagnosis of humoral immunodeficiencies in infants and young children.
D000328:Adult; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D007153:Immunologic Deficiency Syndromes / Q000097:blood / Q000276:immunology*; D007223:Infant; D007231:Infant, Newborn; D008214:Lymphocytes / Q000276:immunology*
D007074:Immunoglobulin G; D007136:Immunoglobulins