Effects of proteolytic encymes on uremic serum inhibition of hepatic and renal transport functions.
Dialysates of rat uremic serum were incubated with Pronase, Carboxypeptidase A, Leucine Aminopeptidase, or Trypsin to investigate whether these enzymes might destroy the inhibitory effects of the dialysates on the uptake of paraaminohippurate by rat renal cortical slices or the uptake of purines by rat liver slices. Of these enzymes, Pronase produced the greatest destruction (63.2 +/- 4.5%) of the inhibitory action on renal transport, with a much smaller destruction (17.8 +/- 2.1%) of the inhibitory effect on hepatic transport.
D000618:Aminohippuric Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D001769:Blood / Q000187:drug effects; D002268:Carboxypeptidases / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007931:Leucyl Aminopeptidase / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010455:Peptides / Q000494:pharmacology*; D011402:Pronase / Q000494:pharmacology; D011687:Purines / Q000378:metabolism; D051381:Rats; D013329:Structure-Activity Relationship; D014357:Trypsin / Q000494:pharmacology; D014511:Uremia / Q000097:blood*
D000618:Aminohippuric Acids; D010455:Peptides; D011687:Purines; D002268:Carboxypeptidases; D007931:Leucyl Aminopeptidase; D014357:Trypsin; D011402:Pronase