Effect of chloramphenicol on early mRNA synthesis in bacteriophage lambda.
The N gene product induces lambda-delayed early mRNA synthesis. This transcription was sensitive to chloramphenicol at the very early stage of phage development, but became resistant within 2-4 min after infection. However, the same transcription was sensitive to the antibiotic throughout phage development if lambda tof- mutant was used as the infecting phage. The results suggest involvement of the tof gene in lambda early transcription; the tof product may act as a stabilizer of the N gene function.
D002701:Chloramphenicol / Q000494:pharmacology*; D003090:Coliphages / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial; D005796:Genes; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects*
D012333:RNA, Messenger; D002701:Chloramphenicol