Liquid chromatographic separation and detection of nanogram quantities of biologically important dicarboxylic acids.
Phenacyl and benzyl derivatives of some biologically significant dicarboxylic acids have been prepared in a quantitative manner using a crown ether catalyst. They were separated using reversed-phase chromatography. The column used was packed with Corasil II to which a C9 phase (nonyl) was bonded. Water-methanol (68:32) was used as the mobile phase. Detection limits in the range of 5--15 ng have been obtained for a non-optimized system. The ease of preparation of these derivatives, coupled with their excellent chromatographic properties, makes this a very attractive procedure for the investigation of mixtures containing biologically significant acids. The implications and future of this technique are discussed.
D000478:Alkylation; D001593:Benzyl Compounds / Q000032:analysis; D002384:Catalysis; D002853:Chromatography, Liquid / Q000379:methods*; D003998:Dicarboxylic Acids / Q000032:analysis*; D004987:Ethers / Q000302:isolation & purification; D008832:Microchemistry; D010648:Phenylacetates / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D001593:Benzyl Compounds; D003998:Dicarboxylic Acids; D004987:Ethers; D010648:Phenylacetates