Separation of Dirofilaria immitis allergen from the IgG-inducing antigens.
Allergen in crude extract of Dirofilaria immitis was purified and separated from the IgG-inducing antigens by a combination of DEAE-Sephadex A-50 chromatography and Sephadex G-200 gel filtration. The molecular weight of the purified preparation was estimated to be approximately 20,000 by gel filtration. The carbohydrate content of the preparation was apparently low, about 2%. Rats immunized with the allergenic fraction developed only a homocytotropic antibody and no hemagglutination antibody.
D000485:Allergens / Q000302:isolation & purification*; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000032:analysis*; D004183:Dirofilaria immitis / Q000276:immunology*; D005260:Female; D005370:Filarioidea / Q000276:immunology*; D007111:Immunity, Cellular; D007074:Immunoglobulin G; D007518:Isoantibodies / Q000032:analysis; D008297:Male; D011817:Rabbits; D051381:Rats
D000485:Allergens; D000941:Antigens; D007074:Immunoglobulin G; D007518:Isoantibodies