[The lysis of proteins with cyanogen bromide (author's transl)].
Bovine beta-lactoglobulin-AB was split with cyanogen bromide, and the reaction mixture was analyzed by countercurrent distribution, gel chromatography and finally, chromatography on phosphocellulose. In addition to the previously described splitting products, we obtained three more minor products, with yields of 8, 15 and 25%. The analytical data indicate that these were formed by lysis C-terminal from the tryptophan-19 and the tryptophan-61 of beta-lactoglobulin. The result is discussed.
D000596:Amino Acids; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D003377:Countercurrent Distribution; D003488:Cyanogen Bromide; D007782:Lactoglobulins
D000596:Amino Acids; D007782:Lactoglobulins; D003488:Cyanogen Bromide
Uber die Spaltung von Eiweissstoffen mit Bromcyan