Studies on norepinephrine-induced efflux of free fatty acid from hamster brown-adipose-tissue cells.
Cells were isolated from brown adipose tissue of warm-adapted hamsters and the fate of free fatty acids released during norepinephrine-induced lipolysis was investigated. The isolated resting cells contain between 100-400 nmoles cell-associated free fatty acids per 10(6) cells; most preparations contained about 200 nmoles/10(6) cells. During norepinephrine-stimulated lipolysis, the level of cell-associated free fatty acids remains constant or decreases gradually, but does not increase, while the concentration of extracellular fatty acids increases linearly. The rate of norepinephrine-stimulated efflux of free fatty acids was 40 +/- 20 nmol X min-1 X 10(6) cells-1 (n = 11) at 37 degrees C. The data strongly indicate that brown adipose tissue can supply free fatty acids to the circulatory system in hamster.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D006224:Cricetinae; D003847:Deoxyglucose / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D009584:Nitrogen / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D010169:Palmitic Acids / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D005230:Fatty Acids, Nonesterified; D010169:Palmitic Acids; D012710:Serum Albumin, Bovine; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D000255:Adenosine Triphosphate; D003847:Deoxyglucose; D009584:Nitrogen; D009638:Norepinephrine