[Study of the fructosediphosphate aldolase and transketolase activity in the nystatin producer, Actinomyces noursei].
Activity of transketolase, an enzyme of the pentose cycle and fructosodiphosphataldolase, an enzyme of glycolisis was studied in the dynamics of development of the nystatin-producing organism and its inactive mutant under various conditions of their cultivation with a purpose of finding relation between the antibiotic production and general metabolism of Act. noursei. The transketolase activity of the organism was 2-4 times higher than that of the inactive mutant. Addition of 8000 Units/ml of nystatin to the medium markedly suppressed (50-100 per cent) the aldolase activity, however it had no effect on the transkelotase activity. Possibly the antibiotic accumulated in the mycelium played the role of a regulator of the activity of the enzymes, directing the metabolites along the hexosomonophosphate pathway of carbohydrate dissimilation.
D000332:Aerobiosis; D003470:Culture Media; D004789:Enzyme Activation; D004794:Enzyme Repression / Q000187:drug effects; D005634:Fructose-Bisphosphate Aldolase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009154:Mutation; D009761:Nystatin / Q000096:biosynthesis* / Q000494:pharmacology; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development; D014174:Transketolase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D003470:Culture Media; D009761:Nystatin; D014174:Transketolase; D005634:Fructose-Bisphosphate Aldolase
Izuchenie aktivnosti fruktozodifosfatal'dolazy i transketolazy u produtsenta nistatina--Actinomyces noursei