9 S thyroid particulate iodoprotein.
Particulate iodoproteins have been studied in the rat thyroid gland using the isotopic 125I equilibration method. Pulse experiments were also performed with a second isotope, 131I. Labelled iodoproteins, both soluble and solubilized by digitonin from the thyroid particulate material, were analyzed by sucrose-gradient ultracentrifugation. 1. At isotopic equilibrium and irrespective of the iodide content of the diet two particulate iodoproteins, with sedimentation coefficients of 27 and 19 S, were solubilized by digitonin. In addition a 9 S iodoprotein was also present but its proportion varied markedly with the iodine content of the diet: it accounted for 50-60% of the label found at the particulate level when the dialy diet of iodine was high (75-500 mug/day) but was almost absent when the diet was only 2 mug/day. 2. Most of this 9 S protein was found in the 600-15 000 times g particulate pellet, i.e. a fraction enriched in lysosomes and phagolysosomes. 3. The iodoamino acid composition of the 9 S fraction was very similar to that of the 19 S particulate thyroglobulin: its hormone content was 19%. 4. The double precipitation technique showed that the 9 S fraction is immunochemically related to thyroglobulin. 5. Pulse experiments showed that the 9 S particulate iodoprotein was slowly labelled by 131I. 6. The amount of 9 S iodoprotein was increased by thyrotropin (30-40% increase versus control experiments 5 min after thyrotropin injection). These properties of the 9 S particulate iodoprotein are discussed in relation to the assumption that it might be a product of partial proteolysis of thyroglobulin after endocytosis and partial digestion by the phagolysosomes.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004072:Digitonin; D007454:Iodides / Q000032:analysis; D007467:Iodoproteins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D008297:Male; D051381:Rats; D013347:Subcellular Fractions / Q000032:analysis; D013961:Thyroid Gland / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D013972:Thyrotropin / Q000494:pharmacology
D000596:Amino Acids; D007454:Iodides; D007467:Iodoproteins; D013972:Thyrotropin; D004072:Digitonin