Enzymatic synthesis of glucocerebroside by UDP-glucose: ceramide glucosyltransferase during ontogenesis of chicken retina.
The activity of uridine diphosphate-glucose: ceramide glucosyltransferase during chick retina ontogenesis was studied. This enzyme catalyzes the first step in the biosynthetic pathway leading to gangliosides. The glucosyltransferase activity was detected in 8 day old embryos, and was present in adult animals. The highest specific activity was found ca. day 10 of embryonic life. The radioactive product was identified as glucocerebroside by means of radiochromatography. The presence of phosphatidylcholine in the incubation mixtures was found to stimulate the enzyme.
D000367:Age Factors; D000818:Animals; D002518:Ceramides; D002554:Cerebrosides / Q000096:biosynthesis*; D002642:Chick Embryo; D002645:Chickens; D005964:Glucosyltransferases / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000494:pharmacology; D012160:Retina / Q000201:enzymology* / Q000254:growth & development; D014532:Uridine Diphosphate Glucose
D002518:Ceramides; D002554:Cerebrosides; D010713:Phosphatidylcholines; D005964:Glucosyltransferases; C023246:ceramide glucosyltransferase; D014532:Uridine Diphosphate Glucose