[Vergleichende Untersuchungen mit (14c)5-Aminolävulinat und (14c)Uroporphyrinogen (author's transl)].
Cell-free extracts from Clostridium tetanomorphum, a microorganism which synthesizes corrins but no heme, are capable of converting both 5-aminolevulinate and uroporphyrinogen III into cobyrinic acid. Comparative examinations with (14C)5-aminolevulinate and (14C)uroporphyrinogen yielded corresponding results. Cell-free extracts from Clostridium tetanomorphum contain uroporphyrinogen III. To obtain good radiochemical yields it is therefore necessary to use substrates of high specific radioactivity. A method for the preparation of 14C-labelled uroporphyrin I-IV with high specific radioactivity is described.
D000622:Aminolevulinic Acid / Q000378:metabolism*; D002474:Cell-Free System; D003013:Clostridium / Q000378:metabolism*; D007982:Levulinic Acids / Q000378:metabolism*; D011165:Porphyrinogens / Q000378:metabolism*; D014577:Uroporphyrinogens / Q000378:metabolism*; D014578:Uroporphyrins / Q000138:chemical synthesis; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000096:biosynthesis
D007982:Levulinic Acids; D011165:Porphyrinogens; D014577:Uroporphyrinogens; D014578:Uroporphyrins; C010895:cobyrinic acid; D000622:Aminolevulinic Acid; D014805:Vitamin B 12
Bildung von Cobyrinsäure mittels eines zellfreien Systems aus Clostridium tetanomorphum