Action of phospholipases on the phosphatidylcholine exchange protein from beef liver.
The phospholipases A2, C and D have been used to investigate the localization of phosphatidylcholine in the phosphatidylcholine exchange protein from beef liver. The rate of enzymatic hydrolysis of the protein-bound phosphatidylcholine was found to be very low. Addition of deoxycholate, isobutanol or dioxane to the native protein, under conditions where delipidation did not occur, greatly enhanced the hydrolytic action of the phospholipases. From these results it is concluded that phosphatidylcholine may be buried in the protein molecule.
D000818:Animals; D001693:Biological Transport, Active; D000440:Butanols / Q000494:pharmacology; D002417:Cattle; D003840:Deoxycholic Acid / Q000494:pharmacology; D004146:Dioxanes / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D010740:Phospholipases / Q000494:pharmacology*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D011955:Receptors, Drug / Q000187:drug effects*
D000440:Butanols; D004146:Dioxanes; D010713:Phosphatidylcholines; D011506:Proteins; D011955:Receptors, Drug; D003840:Deoxycholic Acid; D010740:Phospholipases