[Molecular weight characteristics of the proteins of oocytes and of cleavage ova in CBA mice by means of capillary microdisc electrophoresis].
5-20 oocytes or cleaving embryos of CBA mice were dissolves in 0.2-0.5 ml of 1% buffered sodium dodecylsulfate (SDS), and soluble proteins were separated using electrophoresis in capillaries filled with PAA-SDS-gel. The method enabled us to calculate the approximate molecular weight of proteins by relative mobilities and allowed to determine 1-5 mmcg protein in a single band. 7 groups of proteins were identified in mouse oocytes using 10% PAA-0.1% SDS-gel with the following molecular weights: 208 000, 206 000, 155 000, 112 000, 59 000, 40 000 and 30 000, resp. The same groups of protein were discovered in zygotes and cleaving mouse embryos. The molecular weight distribution of low-molecular weight basic proteins of CBA mice is species-specific and reveals a qualitative changes in the early embryogenesis.
D000818:Animals; D002455:Cell Division; D004527:Egg Proteins / Q000032:analysis*; D004589:Electrophoresis, Disc / Q000379:methods*; D005260:Female; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D009865:Oocytes / Q000032:analysis*; D010063:Ovum / Q000032:analysis*; D011247:Pregnancy; D051381:Rats; D012995:Solubility; D013045:Species Specificity; D015053:Zygote / Q000032:analysis
D004527:Egg Proteins
Molekuliarno-vesovaia kharakteristika belkov ootsitov i drobiashchikhsia iaĭtsekletok mysheĭ CBA metodom kapilliarnogo mikrodiskelektroforeza