Human luteinizing hormone. Isolation and characterization of the native hormone and its alpha and beta subunits.
A new procedure is described for the isolation of the alpha and beta chains of the hormone. In this method, thenative hormone is incubated in acidic urea and the chains are then separated by ion-exchange chromatography. The amino-terminal residue of the alpha subunit is valine. The carboxy-terminal end of the alpha subunit is of variable length. No amino-terminal residue was detected for the beta chain; glycine was found at its carboxy-terminal end by the selective titration method. The amino acid and carbohydrate compositions of the hormone and both subunits are presented. The beta chain contains sialic acid and is devoid of galactosamine in contrast to the beta subunits of other species. Contamination of our human lutenizing hormone preparation by other pituitary glycoprotein hormones such as thyroid-stimulating hormone and follicle-stimulating hormone amounted to 0.5 and 0.25 percent by weight respectively. Cross-contamination of the initial alpha and beta subunit preparations was measured by specific radioimmunoassays and amounted to 4.1 and 2 percent by weight respecitively. Further extensive purification of these subunit preparations was then performed by means of affinity chromatography using immunosorbants. The final preparations exhibited a residual cross-contamination amounting to 0.2 and 0.02 percent by weight for the alpha and beta subunits respectively.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001681:Biological Assay; D002846:Chromatography, Affinity; D005260:Female; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006801:Humans; D007986:Luteinizing Hormone / Q000032:analysis* / Q000276:immunology / Q000494:pharmacology; D046911:Macromolecular Substances; D010053:Ovary / Q000187:drug effects; D010902:Pituitary Gland / Q000032:analysis; D011863:Radioimmunoassay; D012794:Sialic Acids / Q000032:analysis
D000596:Amino Acids; D006595:Hexosamines; D006601:Hexoses; D046911:Macromolecular Substances; D012794:Sialic Acids; D007986:Luteinizing Hormone