The failure of methotrexate to inhibit chicken fibroblast proliferation in a serum-containing culture medium.
Methotrexate (1 muM) abolishes the proliferation of chicken fibroblasts in a culture medium containing defibrinogenated chicken plasma but does not affect proliferation in a medium containing chicken serum.
D000818:Animals; D001769:Blood; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D002645:Chickens; D003470:Culture Media; D005340:Fibrinogen; D005347:Fibroblasts / Q000187:drug effects*; D005492:Folic Acid; D006358:Hot Temperature; D008727:Methotrexate / Q000494:pharmacology*; D010949:Plasma
D003470:Culture Media; D005340:Fibrinogen; D005492:Folic Acid; D008727:Methotrexate