More sensitive automated detection of polyamines in physiological fluids and tissue extracts with omicron-phthalaldehyde.
Omicron-Phthalaldehyde reacts with primary amines to form an intensely blue-fluorescing product. Replacing ninhydrin with this compound in our automated high-pressure liquid-chromatographic technique for determination of polyamines, we achieved a 6- to 10-fold increase in sensitivity. Linearity and reproducibility are excellent, and the method is shown to be applicable to analysis of physiological fluids and of tissue extracts.
D000447:Aldehydes; D000653:Amniotic Fluid / Q000032:analysis; D000818:Animals; D001322:Autoanalysis; D001932:Brain Neoplasms / Q000032:analysis; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008832:Microchemistry; D009374:Neoplasms, Experimental / Q000032:analysis; D010795:Phthalic Acids; D011073:Polyamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D011247:Pregnancy; D011700:Putrescine / Q000032:analysis; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis
D000447:Aldehydes; D007202:Indicators and Reagents; D010795:Phthalic Acids; D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine