Coagulation in an agarose gel and its application to the detection and measurement of factor VIII antibodies.
Evidence has been presented that fibrin formation may be detected directly in agarose gels containing citrated plasma when they are treated with thrombin or calcium chloride. A new assay is described for haemophilic factor-VIII antibody based on inhibition of fibrin formation within an agarose gel containing normal plasma.
D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D001780:Blood Coagulation Tests / Q000379:methods; D002122:Calcium Chloride / Q000378:metabolism; D005169:Factor VIII / Q000276:immunology*; D005337:Fibrin / Q000032:analysis; D005782:Gels; D006467:Hemophilia A / Q000097:blood / Q000276:immunology; D006801:Humans; D011134:Polysaccharides; D012685:Sepharose; D013997:Time Factors
D000906:Antibodies; D005782:Gels; D011134:Polysaccharides; D005169:Factor VIII; D005337:Fibrin; D012685:Sepharose; D002122:Calcium Chloride