Growth of the haploid phase of the myxomycete Physarum flavicomum in defined minimal medium.
The haploid phase (myxamoebae-swarm cells) of the myxomycete Physarum flavicomum grew readily in chemical defined liquid media. The minimal medium contained salts, glucose, biotin, thiamine, hematin, glycine, L-arginine and L-methionine. Cell yields of 1.4 times 10(7) cells/ml were obtained in this medium in aerobic shake culture. These cells consumed about 35 muliters of oxygen/mg protein-hr in the minimal medium. The morphology of cells maintained in this medium appeared to be "normal". L-valine replaced either glycine or L-methionine in the minimal medium but the growth rates and cell yields were reduced. Growth rates increased in media containing four, seven, or fourteen amino acids.
D000596:Amino Acids; D001710:Biotin; D003470:Culture Media; D006238:Haploidy; D006418:Heme; D009235:Myxomycetes / Q000254:growth & development*; D010101:Oxygen Consumption; D010804:Physarum / Q000254:growth & development* / Q000378:metabolism; D013831:Thiamine
D000596:Amino Acids; D003470:Culture Media; D006418:Heme; D001710:Biotin; D013831:Thiamine