Isobaric titration of readine monolayers: kinetics of hydrolysis of glycerides by pancreatic lipase B.
The rate of lipolytic enzyme-catalyzed reactions yielding water-soluble products can be measured by isobaric titration. The method is based upon the measurement of the amount of substrate that must be added to a monalayer to maintain constant surface pressure during the course of the enzymatic reaction. The rate constants determined for the hydrolysis of trioctanion and 1,2-diotanoin by pancreatic lipase were identical with those determined by the variable surface pressure method and by a radioactive substrate technique. This direct titrimetric method has a wider dynamic range and more versatility for following surface reactions that previously described systems.
D000818:Animals; D002210:Caprylates; D002250:Carbon Radioisotopes; D055598:Chemical Phenomena; D002621:Chemistry; D004075:Diglycerides / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005989:Glycerides / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D008433:Mathematics; D008567:Membranes, Artificial; D008722:Methods; D010179:Pancreas / Q000201:enzymology*; D012995:Solubility; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013552:Swine; D014280:Triglycerides / Q000378:metabolism
D002210:Caprylates; D002250:Carbon Radioisotopes; D004075:Diglycerides; D005989:Glycerides; D008567:Membranes, Artificial; D014280:Triglycerides; D008049:Lipase