Sindbis virus glycoproteins: effect of the host cell on the oligosaccharides.
Sindbis virus was grown in four different host cells and the carbohydrate portions of the glycoproteins were analyzed. Sindbis virus grown in BHK-21 cells has more sialic acid and galactose than Sindbis virus grown in chicken embryo cells. In other respects the carbohydrates from virus grown in these two hosts are very similar. Sindbis virus grown either in chick cells transformed by Rous sarcoma virus or in BHK cells transformed by polyoma virus was also examined. In comparisons of virus from normal and transformed cells, differences in the amount of sialic acid were observed; but otherwise the carbohydrate structures appeared basically similar. The growth conditions used for the host cell also affected the degree of completion of the carbohydrate chains of the viral glycoproteins.
D000606:Amino Sugars / Q000032:analysis; D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D006224:Cricetinae; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis*; D009439:Neuraminidase / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis / Q000096:biosynthesis*; D012794:Sialic Acids / Q000032:analysis; D012845:Sindbis Virus / Q000378:metabolism*; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D000606:Amino Sugars; D006023:Glycoproteins; D009844:Oligosaccharides; D012794:Sialic Acids; D014764:Viral Proteins; D009439:Neuraminidase; D005944:Glucosamine; D005690:Galactose