Studies on the biosynthesis of basic 16-membered macrolide antibiotic, platenomycins. I. Selection of and cosynthesis by non-platenomycin-producing mutants.
In a search for blocked mutants which may produce a biosynthetic intermediate, mutation by N-methyl-N'-nitro-N-nitrosoguanidine treatment and/or ultra-violet irradiation were performed on platenomycin-producing Streptomyces platensis subsp. malvinus MCRL 0388 (NRRL 3761). Twenty four non-platenomycin-producing stable mutants were thus obtained and tested for cosynthesis ability. Antibiotic cosynthesis with a pair of these mutants made it possible to detect the producer of an intermediate. Among these mutants which were classified into eight groups (A to G and doubtful groups), mutants of groups A and B appeared from their complementation pattern to be the useful producers of biosynthetic intermediates of platenomycin.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D006027:Glycosides / Q000096:biosynthesis; D007783:Lactones / Q000096:biosynthesis; D009154:Mutation; D009604:Nitrosoguanidines / Q000494:pharmacology; D011830:Radiation Effects; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D000900:Anti-Bacterial Agents; D006027:Glycosides; D007783:Lactones; D009604:Nitrosoguanidines