Microchromatography of hemoglobins. II. A simplified procedure for the determination of hemoglobin A2.
A modification of the microchromatographic procedure for Hb-A2 which utilizes DEAE-cellulose (Reference 1) allows the quantitation of Hb-A2 without interference from any Hb-S in the sample. Elution of Hb-A2 with a glycine-KCN developer is much less sensitive to minor change in pH of the developer, but is greatly dependent on the pH of the ion exchanger.
D002848:Chromatography, DEAE-Cellulose / Q000379:methods*; D006452:Hemoglobinometry / Q000379:methods*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans
D006455:Hemoglobins, Abnormal