Testosterone metabolism in benign and malignant breast lesions.
Tissues from a variety of breast lesions were incubated with 14C-testosterone (17beta-hydroxy-4-androsten-3-one). Conversion to 14C-5alpha-dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one) and 14C-androstenedione (4-androsten-3,17-dione) was measured. Normal breast tissue showed formation of 14C-androstenedione but no formation of 14C-5alpha-dihydrotestosterone. Fibroadenomas showed a high degree of testosterone metabolism forming both 14C-androstenedione and 14C-5alpha-dihydrotestosterone. The adenocarcinomas of the breast, contrary to a previous report in the literature, showed no conversion to 14C-5alpha-dihydrotestosterone. The data suggest that formation of 5alpha-dihydrotestosterone is a predominant metabolic pathway in fibroadenoma.
D000230:Adenocarcinoma / Q000378:metabolism*; D000232:Adenofibroma / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000735:Androstenedione / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D013196:Dihydrotestosterone / Q000378:metabolism; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D066298:In Vitro Techniques; D008413:Mastitis / Q000378:metabolism*; D008722:Methods; D008875:Middle Aged; D011247:Pregnancy; D013739:Testosterone / Q000378:metabolism*
D002250:Carbon Radioisotopes; D013196:Dihydrotestosterone; D013739:Testosterone; D000735:Androstenedione