Temperature-sensitive cell cycle mutants: a chinese hamster cell line with a reversible block in cytokinesis.
A thermosensitive line (TS 111) was isolated from a suspension culture of Chinese hamster fibroblasts, using a BUdR suicide selection technique. In this line, cytokinesis is blocked at 39 degrees C. DNA and protein synthesis are not arrested but keep on at a steady rate. Giant cells are produced which accumulate either numerous nuclei or one big nucleus with several nucleoli and more than a hundred chromosomes. At each nuclear cycle, all the chromosomes in the cell appear to condense in a synchronous manner, although it is possible that not all the sets of chromosomes duplicate. When the culture is returned to the permissive temperature (34 degrees C) after a prolonged arrest at the restrictive temperature, cytokinesis resumes with early extrusion of karyoplasts from multinucleated cells. The division block is independent of cell density in suspension culture and is not prevented by cell contact when cells grow attached to Petri dishes. At 34 degrees C, a residual expression of the mutation is indicated by the presence of binucleate and up to 30% anucleate cells. A remarkable similarity and some synergism exists between the mutation and cytochalasin B effects.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure; D006224:Cricetinae; D003571:Cytochalasin B / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005020:Ethyl Methanesulfonate; D005347:Fibroblasts; D007621:Karyotyping; D009153:Mutagens; D009154:Mutation; D014176:Protein Biosynthesis; D013696:Temperature
D009153:Mutagens; D003571:Cytochalasin B; D004247:DNA; D005020:Ethyl Methanesulfonate; D001973:Bromodeoxyuridine