Isolation of a concanavalin A receptor from mouse L cells.
A cell surface glycoprotein receptor for concanavalin A (Con A) has been isolated from mouse L cells. The isolation procedure involved dissolving whole L cells in 0.3 M lithium diiodosalicylate and extracting with aqueous phenol. The Con A receptor, which was found in the aqueous phase of this extract, was further purified by affinity chromatography on a column of Con A-Sepharose; the receptor was adsorbed to Con A-Sepharose and eluted with 0.1 M methyl alpha-D-glucopyranoside or with 0.1 M methyl alpha-D-mannopyranoside, but not with other monosaccharides. The cell surface location of the Con A receptor purified in this way was confirmed by showing that it can be isolated from purified L cell plasma membranes and by demonstrating that it can be labeled from the exterior surface of intact L cells by the nonpenetrating galactose oxidase-KB3H4 system. Biochemical studies of the Con A receptor have shown that it migrates on sodium dodecyl sulfate-polyacrylamide gels as a single component having an apparent molecular weight of approximately 100,000. Its N-terminal amino acid is valine and it has carbohydrate attached at several (at least five) different sites along the polypeptide chain.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D007739:L Cells / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D011485:Protein Binding; D011955:Receptors, Drug
D000596:Amino Acids; D006023:Glycoproteins; D011955:Receptors, Drug; D003208:Concanavalin A