Dengue virus plaque formation on microplate cultures and its application to virus neutralization (38564).
Dengue virus plaque formation on BHK-21 cell microplate cultures was described.  The clear plaques were visible usually 5 days after incubation in a CO-2 incubator at 37 degrees.  The cells cultured in a 3-oz bottle were sufficient to prepare two microculture plates which were usually ready for use after 1-2 days of cultivation in the CO-2 incubator at 37 degrees.  The overall procedures were easy and of economic advantage.
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification*; D007668:Kidney; D009500:Neutralization Tests; D013997:Time Factors; D010948:Viral Plaque Assay / Q000379:methods*; D014776:Virus Cultivation / Q000379:methods*