Purification of murine thymus leukemia antigen (TL). A quantitative assessment of limited proteolysis.
The murine thymus leukemia antigen (TL) has been solubilized from the tumor ASL1 and from an established cell line ASL1W, by papain digestion. When a 15-min digest was chromatographed on Sephadex G-200, two peaks of TL activity were eluted with apparent molecular weights of approximately 58,000 and 31,000. Chromatography of a 30-min digest under the same conditions resulted in elution of a single peak of activity with an apparent molecular weight of 58,000. Additional purification was carried out on the 58,000 molecular weight material by absorption to, and elution from DEAE-cellulose. The combination of gel filtration and ion exchange chromatography resulted in approximately a 150-fold purification.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D002460:Cell Line; D002470:Cell Survival; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D007942:Leukemia, Experimental / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D010206:Papain; D013950:Thymus Gland / Q000032:analysis / Q000276:immunology*
D000941:Antigens; D010206:Papain