Polysome isolation of sepharose column chromatography.
Polysomes from the mouse myeloma MOPC-21 were purified by gel filtration of Sepharose 6B, 4B and 2B columns. All three columns eliminated nearly all intracellular material smaller than 40 S subunits. In addition, passage through 4B and 2B columns substantially reduced the amount of subunits and monosomes in the preparations. Purified polysomes retained structural integrity when stored at -85 degrees C for at least nine weeks.
D002458:Cell Fractionation; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel / Q000379:methods; D005615:Freezing; D011132:Polyribosomes / Q000648:ultrastructure*; D012685:Sepharose
D012685:Sepharose