Gas-liquid chromatographic and mass fragmentographic determination of 3-O-methylated catecholamines in human plasma.
Plasma 3-O-methylated catecholamines, i.e. 3-methoxytyramine, normetanephrine and metanephrine, were separated from catecholamines by passing through alumina and further purified by adsorbing on weakly acidic resin and Amberlite XAD-4. The amines were trifluoroacetylated and determined by gas chromatography or mass fragmentography. Tracer quantities of tritiated 3-MT, NMN or MN were used as internal standards for total recovery estimations. The contents of 3-O-methylated catecholamines in the plasma of normal persons and patients with hyperthyroidism, hypertension, neuroblastoma and pheochromocytoma were measured.
D000328:Adult; D000368:Aged; D002395:Catecholamines / Q000097:blood*; D002648:Child; D002849:Chromatography, Gas / Q000379:methods; D002852:Chromatography, Ion Exchange; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006973:Hypertension / Q000097:blood; D006980:Hyperthyroidism / Q000097:blood; D008297:Male; D008676:Metanephrine / Q000097:blood; D008832:Microchemistry; D009447:Neuroblastoma / Q000097:blood; D009647:Normetanephrine / Q000097:blood; D010673:Pheochromocytoma / Q000097:blood; D013329:Structure-Activity Relationship; D014439:Tyramine / Q000031:analogs & derivatives / Q000097:blood
D002395:Catecholamines; D009647:Normetanephrine; D008676:Metanephrine; D014439:Tyramine