Early onset of serum blocking in a murine melanoma model.
The early onset of serum blocking of host cell-mediated immunity was investigated in a transplantable murine melanoma employing two in vitro assays. One of these was based on the Takasugi and Klein cytotoxicity test; the other was a new test based on the inhibition of adherence of leukocytes to a plastic surface in the presence of tumor antigen. Both tests yielded similar results, and the adherence inhibition test was more sensitive. Blocking could be detected as early as 1 h after the inoculation of 1 times 10(7) melanoma cells. Early onset of serum blocking was also demonstrable if homogenized of tissue-cultured tumor cells were inoculated. Serum blocking under these circumstances was initially transient, declining at 24 to 48 h, but reappearing by the 4th day after inoculation. Furthermore, a functional immune system was not required for blocking factor development in this system, as evidenced by the onset of a similar pattern of blocking activity in heavily irradiated animals.
D000818:Animals; D000912:Antibodies, Neoplasm; D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D007104:Immune Adherence Reaction; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology; D008545:Melanoma / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D014185:Transplantation, Isogeneic
D000912:Antibodies, Neoplasm; D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm