Incorporation of (3H)thymidine into brain DNA after cerebellar damage.
Changes of incorporation of [3H] thymidine into brain DNA were studied in C57BL/6J mice after perinatal neocerebellar lesion. The destruction of part of the left neocerebellar cortex caused temporary increase of the specific radioactivity of DNA extracted from neocerebellum (15.22 +/- 0.57 cpm/mg DNA vs. 4.83 +/- 0.40 cpm/mg DNA in controls), from left hemisphere (9.86 +/- 0.45 cpm/mg DNA in operated vs. 4.22 +/- 0.40 cpm/mg DNA in controls), and from right hemisphere (11.75 +/- 0.52 cpm/mg DNA in operated vs. 4.78 +/- 0.39 cpm/mg DNA in controls). The labeled DNA was localized both in glia and in neurons in different brain areas. In animals operated upon in adult age, no changes in labeling of brain DNA were observed.
D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001927:Brain Diseases / Q000378:metabolism*; D002525:Cerebellar Cortex / Q000473:pathology; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism; D012018:Reflex; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium / Q000378:metabolism
D014316:Tritium; D004247:DNA; D013936:Thymidine