Heparin-sensitive and nonsensitive forms of thrombin.
Two forms of thrombin (Ts-thrombin and Tp-thrombin) were found with respect to heparin sensitivity. Inactivation of Ts-thrombin by antithrombin-III was facilitated with heparin, whereas inactivation of Tp-thrombin was not. Both thrombins were bound to heparin in a Sephadex G-200 gel filtration experiment. Ts-thrombin proved to be more stable and was better protected by heparin against heat inactivation of 54 degrees C than Tp-thrombin.
D000818:Animals; D000991:Antithrombins / Q000502:physiology; D001665:Binding Sites; D002417:Cattle; D002850:Chromatography, Gel; D006493:Heparin / Q000494:pharmacology*; D007700:Kinetics; D011485:Protein Binding; D013917:Thrombin / Q000378:metabolism*; D013997:Time Factors
D000991:Antithrombins; D006493:Heparin; D013917:Thrombin