Human interferon production: superinduction by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole.
Polyinosinic.polycytidylic acid [poly(I.C)] induced production of interferon by a strain of diploid human fibroblasts (FS-4), measured between 5 and 24 hours from induction, is enhanced up to 128-fold by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), a reversible inhibitor of nuclear heterogeneous RNA synthesis. A normalized dose-effect plot shows a close correlation between the superinducing effect of DRB and inhibition of RNA synthesis. Cultures that contained DRB continue to produce interferon for up to 4 days. Removal of the drug at any time during this period leads to a prompt shutoff of interferon production.
D001562:Benzimidazoles / Q000494:pharmacology*; D002478:Cells, Cultured; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D007369:Interferon Inducers / Q000494:pharmacology*; D007372:Interferons / Q000096:biosynthesis*; D011070:Poly I-C / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D012263:Ribonucleosides / Q000494:pharmacology*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
D001562:Benzimidazoles; D007369:Interferon Inducers; D012263:Ribonucleosides; D007372:Interferons; D011070:Poly I-C