Histochemical study of monoamine oxidase latency in the liver of the rat.
In a histochemical test system with adrenaline as substrate and nitroblue tetrazolium (NBT) as electron acceptor, an increase of NBT reduction in rat liver sections was found microspectrophotometrically following short hypotonic treatment. Investigations with iproniazide, a monoamine oxidase inhibitor, and non-enzymatic NBT reduction showed that the increased formazan formation was related to the presence of monoamine oxidase. It is suggested that the reason for the observed increase of formazan formation is due to increased permeability of the inner mitochondrial membrane to NBT. Consequently, the increase of monoamine oxidase observed in the histochemical test system does not represent mobilization of a latent activity, but rather complete assessment of activity that is normally present.
D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000378:metabolism; D005562:Formazans / Q000378:metabolism; D006651:Histocytochemistry; D007038:Hypotonic Solutions; D066298:In Vitro Techniques; D007490:Iproniazid / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D008297:Male; D008566:Membranes; D008930:Mitochondria, Liver / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D009997:Osmotic Pressure; D010084:Oxidation-Reduction; D010539:Permeability; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D013395:Sucrose / Q000494:pharmacology; D014867:Water / Q000494:pharmacology
D005562:Formazans; D007038:Hypotonic Solutions; D014867:Water; D009580:Nitroblue Tetrazolium; D011092:Polyethylene Glycols; D013395:Sucrose; D007490:Iproniazid; D008995:Monoamine Oxidase; D004837:Epinephrine