Studies on interaction between poly(L-lysine58, L-phenylalanine42) and deoxyribonucleic acids.
A random copolymer of 58% L-lysine and 42% L-phenylalanine, poly(Lys58Phe42), was used as a model protein for studying the role of phenylalanine residues in protein-DNA interaction. Complexes between this copolypeptide and DNA, made by direct mixing, were studied by absorbance, circular dichroism (CD), fluorescence, and thermal denaturation. Complex formation results in an increase in absorbance, and an enhancement, red-shift, and broadening of phenylalanine fluorescence. The fluorescence enhancement is opposite to the quenching observed when a tyrosine copolypeptide is bound to DNA (R. M. Santella and H.J. Li (1974), Biopolymers 13, 1909). The positive CD band of DNA near 275 nm is reduced and red-shifted by the binding of the phenylalanine copolypeptide to a greater extent than by the tyrosine copolypeptide. Thermal denaturation of the complexes in 2.5 times 10(-4) M EDTA (pH 8.0) shows three characteristic melting bands. For complexes with calf thymus DNA, free base pairs melt at Tm,I (47-49 degrees) and copolypeptide-bound base pairs show two melting bands (Tm,II at 73-75 degrees, and Tm,III at 88 -90 degrees). Similar thermal denaturation results have been observed for complexes with Micrococcus luteus DNA. The fluorecence intensity of the complexes is greatly increased when the temperature is raised to the Tm,II region. In addition to fluorescence measurements, the effects of increasing temperature on absorption and CD spectra of the complexes were also studied. Stacking interaction between the phenylalanine chromophore and DNA bases, either partial or full intercalation, is implicated by the experimental results. Several mechanisms are proposed to describe the reaction between the copolypeptide and DNA, and thermal denaturation of the complex.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA; D004269:DNA, Bacterial; D006358:Hot Temperature; D007700:Kinetics; D046911:Macromolecular Substances; D008433:Mathematics; D008837:Micrococcus; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D010455:Peptides; D010649:Phenylalanine; D011107:Polylysine; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet
D004269:DNA, Bacterial; D046911:Macromolecular Substances; D010455:Peptides; D011107:Polylysine; D010649:Phenylalanine; D004247:DNA