Purification of DNP specific antibodies using sepharose bound DNP-para-aminobenzoylglutamate.
An immunoabsorbent column employing a DNP derivative having restricted molecular freedom (dinitrophenylated-para-aminobenzoylglutamate--Sepharose 4B) was prepared in order to isolate and purify DNP specific antibodies in high yield. The antibodies were recovered from the immunoabsorbent column in yields from 80 to 90% using 3 M sodium thiocyanate, and these antibodies retained their immunologic activity. There appeared to be limited selection of antibodies with specific affinities as was noted in a parallel experiment comparing a second antibody purification procedure.
D000327:Adsorption; D062365:Aminobenzoates / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D004140:Dinitrophenols / Q000276:immunology*; D005971:Glutamates / Q000276:immunology*; D005998:Glycine / Q000276:immunology; D006801:Humans; D008722:Methods; D011134:Polysaccharides / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D012685:Sepharose / Q000276:immunology*; D012709:Serum Albumin
D062365:Aminobenzoates; D000906:Antibodies; D004140:Dinitrophenols; D005971:Glutamates; D011134:Polysaccharides; D012709:Serum Albumin; D012685:Sepharose; D005998:Glycine