A DNA-directed DNA polymerase from murine liver mitochondria.
A DNA-directed DNA polymerase has been isolated from murine liver mitochondria. The mitochondrial DNA polymerase is distinguishable from other DNA polymerases found in the nucleus and cytosol of murine cells by several enzymatic and physical properties. It is stimulated 5--6-fold by 0.15 M KCl, does not require a sulfhydryl reducing agent for activity, and is inhibited by ethidium bromide or ATP. The enzyme has a sedimentation coefficient of 8.8 S in the presence of up to 0.5 M KCl, a molecular weight of 150--170000, and utilizes natural templates in the following order of preference: activated DNA (100%), single stranded DNA (24%), and native DNA (5%).
D000818:Animals; D004247:DNA; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D004996:Ethidium / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D008930:Mitochondria, Liver / Q000201:enzymology*; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000494:pharmacology
D008623:Mercaptoethanol; D011189:Potassium Chloride; D004247:DNA; D004254:DNA Nucleotidyltransferases; D004996:Ethidium; D008274:Magnesium; D005033:Ethylmaleimide