5-Hydroxytryptamine-14C and dexamphetamine-14C uptake by fundal sarcolemma preparations: effect of thermal and chemical alterations as indirect approach to the problem of the common receptor.
By submitting a sarcolemma preparation from gastric fundus to thermal (47 degrees C) and chemical (2 M urea) alterations, a significant decrease of the binding of labelled serotonin and dexamphetamine, 40% and 30% respectively, is observed. This finding suggests that the receptors for these two drugs contain a protein moiety, which undergoes a structural and functional modification upon the thermal or chemical procedure employed. The strictly similar behaviour of the binding properties of serotonin and dexamphetamine, in our experimental conditions, gives a strong indirect support to the hypothesis of a common receptor for these two drugs.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003913:Dextroamphetamine / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011955:Receptors, Drug / Q000187:drug effects; D012508:Sarcolemma / Q000187:drug effects / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D013270:Stomach / Q000648:ultrastructure; D013696:Temperature; D014508:Urea / Q000378:metabolism
D011955:Receptors, Drug; D012701:Serotonin; D014508:Urea; D003913:Dextroamphetamine