Microsomal O-demethylation, N-demethylation and aromatic hydroxylation in the presence of bisulfite and dithiothreitol.
Microsomal O-demethylation of p-nitroanisole, N-demethylation of aminopyrine and aromatic hydroxylation of aniline, were not altered by sodium bisulfite or dithiothreitol at concentrations effective for their use as antioxidants. Incorporation of these antioxidants in microsomal metabolism studies should have general utility in preventing decomposition of readily air oxidizable compounds.
D000632:Aminopyrine / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D000840:Anisoles / Q000378:metabolism; D003640:Dealkylation; D004229:Dithiothreitol / Q000494:pharmacology*; D006900:Hydroxylation; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013447:Sulfites / Q000494:pharmacology*
D000814:Aniline Compounds; D000840:Anisoles; D013447:Sulfites; D000632:Aminopyrine; D004229:Dithiothreitol