Non-histone chromosomal proteins easily extractible from chick erythrocytes.
Those non-histone chromosomal proteins which are easily extractible from chick erythrocytes differ substantially from proteins similarly extracted from other tissues of various species. Although a protein P1 was isolated along with histone H1 by extraction of calf thymus chromatin with HC1O4, the same procedure did not extract this protein from chick erythrocyte chromatin of either normal or regenerating blood. Likewise , non-histone proteins extracted with 0.35 M NaCl from calf thymus differed from those of normal chick erythrocytes, which were qualitatively identical but quantitatively inferior to those of regenerating blood. The major protein of about 25 000 molecular weight, totally extracted with 0.35 M NaCl from calf thymus, was not found in chick erythrocyte chromatin, but rather another major protein of about 35 000 molecular weight was partially extracted from erythrocytes.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification*; D002417:Cattle; D002645:Chickens; D002875:Chromosomes / Q000032:analysis*; D004247:DNA / Q000032:analysis; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000032:analysis*; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis / Q000302:isolation & purification*; D012313:RNA / Q000032:analysis; D013950:Thymus Gland / Q000032:analysis
D000596:Amino Acids; D001798:Blood Proteins; D009698:Nucleoproteins; D012313:RNA; D004247:DNA