Isolation of a Sindbis virus variant by passage on mouse plasmacytoma cells.
A variant of Sindbis virus has been isolated by growing a stock of virus, previously passaged on chicken embryo cells, in mouse plasmacytoma (MOPC 315) cells in suspension culture. An indirect immunofluorescence test and infective center assay showed that only a small fraction of cells could be infected by the stock wild-type virus, but that the population of virus accumulating after a few passages on the mouse cells had host-range properties distinct from the stock virus. The mouse-passaged virus retained its virulence for the original host and back-passaging of this virus on chicken cells did not change its newly acquired properties. Thus, this variant appears to be a genetically distinct form of Sindbis that adsorbs to and grows much better than the stock virus on several types of mouse cells including cultures of mouse macrophages.
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D006224:Cricetinae; D014644:Genetic Variation; D007668:Kidney; D007739:L Cells; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma; D012845:Sindbis Virus / Q000254:growth & development / Q000302:isolation & purification*; D013997:Time Factors; D014776:Virus Cultivation; D014779:Virus Replication