Polyribosomes in different stages of the life cycle of the water mold Allomyces arbuscula.
Synchronous gametogenesis in the water mold Allomyces arbuscula is blocked by actinomycin D added at the onset of the process. Formation of the male gametangium can be selectively inhibited by administering actinomycin one hr after the induction of gametogenesis. The polyribosome pattern obtained after density gradient centrifugation remains virtually unchanged throughout gametogenesis until a stage immediately preceding maturation of the gametes. When ribosome from gametes and swarming zygotes are analyzed on gradients, some RNase-sensitive materials is found to band in the heavier portion of the gradient. Its presence suggests that some messenger RNA associated with ribosomes is conserved in the swarming cells. During gametogenesis RNA is de novo synthesized and becomes associated with the polyribosomes.
D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D008411:Chytridiomycota / Q000187:drug effects / Q000254:growth & development*; D003609:Dactinomycin / Q000494:pharmacology; D005658:Fungi / Q000254:growth & development*; D005854:Germ Cells / Q000187:drug effects; D008297:Male; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D011132:Polyribosomes / Q000187:drug effects; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012098:Reproduction / Q000187:drug effects; D012260:Ribonucleases; D013172:Spores, Fungal
D010710:Phosphates; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D003609:Dactinomycin; D012260:Ribonucleases