The tris-catalyzed isomerization of potassium D-glucose 6-O-sulfate.
Potassium D-glucose 6-O-[35S]sulfate was partially converted by Tris (greater than or equal 25 mM, pH 7.5) to 35SO4(2-), fructose, and a 35S-labelled compound which was tentatively identified as fructose 6-O-sulfate. The isomerization reaction was also catalyzed by ethanolamine and 2-dimethylaminoethanol but not by glycine, triethanolamine, or pentaerythritol. The significance of these findings in relation to kinetic studies of the enzyme glycosulfatase is discussed.
D000588:Amines; D005632:Fructose; D005947:Glucose; D007536:Isomerism; D013464:Sulfuric Acids; D014325:Tromethamine
D000588:Amines; D013464:Sulfuric Acids; D014325:Tromethamine; D005632:Fructose; D005947:Glucose