Detection of IgG antibody to deoxyribonucleic acid by the radioallergosorbent test.
IgG antibodies to heat-denatured calf thymus deoxyribonucleic acid (DNA) single-stranded DNA:ss-DNA) in the sera of patients with systemic lupus erythematosus (SLE) could be detected by means of solid-phase radioimmunoassay. Single-stranded DNA coupled covalently to microcrystalline cellulose and 125I-labeled anti-human gamma chain were used for this test. The principle of this test is known as the radioallergosorbent test (RAST). This paper describes the usefulness of the RAST for the detection of human IgG antibody. Specificity of this test was examined by an inhibition test using ss-DNA and double-stranded DNA (ds-DNA). A trial for the detection of IgM and IgA antibodies to ss-DNA was also attempted.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004247:DNA / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000302:isolation & purification; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification; D007141:Immunoglobulin Fc Fragments / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000032:analysis* / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods
D000906:Antibodies; D004277:DNA, Single-Stranded; D007070:Immunoglobulin A; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D004247:DNA