Production of CO2 in the living mouse immediately after injection of 1- or 2-14C acetate.
Three groups of mice, normally fed, fasted and fed after a fasting period are injected intravenously with either 1- or 2-14C acetate. The respiratory 14CO2 as well as that of the liver, the adipose tissue and the carcass are collected after 3 min and the radioactivity measured. A determination is also made of the radioactivity of the tissue fatty acids and, for two groups of mice, of the circulating glucose. A calculation is suggested by which the number of revolutions performed by the acetate C in the Krebs cycle before it is transformed into CO2 can be deduced. The results suggest that the Krebs cycle is very open, that the acetate C found in the glucose has already broken away from the cycle after one revolution and that the C which appears in the form of CO2 has performed an average of only 2.8 to 3.6 revolutions. The results are evaluated as a function of the experimental conditions chosen.
D000085:Acetates / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002952:Citric Acid Cycle; D005215:Fasting; D005227:Fatty Acids / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D012119:Respiration
D000085:Acetates; D002250:Carbon Radioisotopes; D005227:Fatty Acids; D002245:Carbon Dioxide; D005947:Glucose