Unusual increase in mitochondrial glycerolphosphate dehydrogenase activity in primary cultures of rat liver cells.
Primary cultures of neonatal rat liver cells show an increase in the activity of mitochondrial glycerolphosphate dehydrogenase between the second and tenth day of cultivation. At the end of cultivation the activity level exceeded that of liver tissue in vivo. Replacement of normal serum by hypothyroid serum or addition of triiodothyronine to the medium did not influence significantly the enzyme activity in vitro, in contrast to the very marked effects of thyroid hormones observed in vivo.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005347:Fibroblasts; D005993:Glycerolphosphate Dehydrogenase / Q000302:isolation & purification*; D008930:Mitochondria, Liver / Q000201:enzymology*; D011506:Proteins / Q000032:analysis; D051381:Rats; D013385:Succinate Dehydrogenase / Q000302:isolation & purification
D011506:Proteins; D005993:Glycerolphosphate Dehydrogenase; D013385:Succinate Dehydrogenase