Mitogenic effect of fibroblast growth factor on early passage cultures of human and murine fibroblasts.
Fibroblast growth factor (FGF), a polypeptide that has been shown to stimulate division in 3T3 cells, was tested for mitogenic effects on diploid, early-passage cells from human and murine sources. The quantitative assay of [3H]thymidine incorporation into acid-insoluble material showed that FGF at low concentrations (10 minus 9 M) was more effective than additional serum for provoking the initiation of DNA synthesis in human foreskin fibroblasts or mouse fibroblasts maintained in 5 or 10% serum, respectively. The growth of the human fibroblasts was twice as fast in the presence of FGF plus 10% calf serum as it was in the presence of 10% calf serum or 20% fetal calf serum alone. The addition of FGF to primary cultures of mouse fibroblasts in 0.4% serum resulted in a twofold increase in cell number  compared to controls. In contrast to results obtained with 3T3 cells, neither insulin nor a glucocorticoid potentiated the effects of FGF on either human or mouse cells.
D000818:Animals; D001798:Blood Proteins; D001921:Brain; D002417:Cattle; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D003907:Dexamethasone / Q000494:pharmacology; D004171:Diploidy; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008297:Male; D051379:Mice; D008934:Mitogens; D010413:Penis; D010455:Peptides / Q000494:pharmacology*; D010902:Pituitary Gland; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D001798:Blood Proteins; D006133:Growth Substances; D007328:Insulin; D008934:Mitogens; D010455:Peptides; D014316:Tritium; D003907:Dexamethasone; D004247:DNA; D013936:Thymidine