Modifications of cellular RNA-polymerase II after infection with frog virus 3.
RNA-polymerase II extracted from FV3-infected and uninfected BHK cells were compared by measuring their abilities to bind [3-H]-amanitin and ribonucleoside triphosphates. Binding sites for [3-H]-amanitin and the dissociation constant of the complex between [3H]-amanitin and RNA-polymerase II were significantly modified following FV3 infection. The apparent Km's for ribonucleoside triphosphates remained unchanged.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000546:Amanitins / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002474:Cell-Free System; D006224:Cricetinae; D003597:Cytosine Nucleotides / Q000378:metabolism; D004267:DNA Viruses / Q000254:growth & development*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D007668:Kidney; D012313:RNA / Q000096:biosynthesis; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism
D000546:Amanitins; D003597:Cytosine Nucleotides; D014500:Uracil Nucleotides; D014316:Tritium; D012313:RNA; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases