A rapid radioimmunoassay method for plasma luteinizing hormone.
1. A rapid double antibody radioummunoassay method for the determination of plasma luteinizing hormone (hLH) is described. 2. The method involves preincubation of the anti-LH serum with the precipitating second antibody and incubation of this mixture with unknows and standards at room temperature overnight. The incubation tubes are then centrifuged, supernatants decanted and the precipitates counted. 3. A total of two working days is required to complete the assay and report the results. 4. This method is more rapid than the conventional ones currently used for the estimation of LH in blood.
D000818:Animals; D000906:Antibodies; D001665:Binding Sites; D004355:Drug Stability; D005069:Evaluation Studies as Topic; D006041:Goats / Q000276:immunology; D006801:Humans; D007700:Kinetics; D007986:Luteinizing Hormone / Q000097:blood*; D008832:Microchemistry; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D013997:Time Factors
D000906:Antibodies; D007986:Luteinizing Hormone