The solution of pharmacological problems with computers. Part 9: Kinetics of the inhibition of the enzyme morphine-N-demethylase by N-allylnormorphine. Mathematical analysis by a FORTRAN-program.
In this paper, we present a method for statistical analysis of enzyme-kinetic data. This analysis allows determination of the character of the inhibition of an enzyme reaction, and computes the 95% confidence limits of the dissociation constants and of Vmax. This method involves the solution of the Michaelis-Menten equation, (see article for formula), using the Guass-Newton iteration.
D000818:Animals; D003201:Computers; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008956:Models, Chemical; D009020:Morphine; D009269:Nalorphine / Q000494:pharmacology*; D010089:Oxidoreductases, N-Demethylating / Q000037:antagonists & inhibitors*; D051381:Rats
D009020:Morphine; D010089:Oxidoreductases, N-Demethylating; D009269:Nalorphine