The separation of S-carboxymethylated human fibrinogen chains with a single carboxymethyl-cellulose chromatography.
A single chromatography on CM 52 cellulose was sufficient to separate the S-carboxymethylated chains of human fibrinogen with a variable gradient mixer. The purity of the chains was confirmed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Approximately 36 percent of the S-carboxymethylated fibrinogen was recovered as the purified chains.
D002266:Carboxymethylcellulose Sodium; D002852:Chromatography, Ion Exchange; D005340:Fibrinogen / Q000302:isolation & purification*; D006801:Humans; D010446:Peptide Fragments / Q000302:isolation & purification
D010446:Peptide Fragments; D005340:Fibrinogen; D002266:Carboxymethylcellulose Sodium