Separation of pineal extracts by gelfiltration. III. Sheep pineal factors acting either on the hypothalamus, or on the anterior hypophysis of mice and rats in in vitro experiments.
Some compounds, other than melatonin, can be detected in aqueous extracts of sheep pineals, using in vitro bioassays. Mild and simple separation methods are used such as gelfiltration on Sephadex G-25, ultrafiltration through Amicon ultramembranes UM-2and UM-05, followed by gelfiltration on Sephadex G-10 and paper electrophoresis. Some fractions are acting on the hypothalamus, others on the anterior hypophysis of mice and rats. The results are compared with those obtained previously with different extraction and separation methods. Key words: Pineal extract, pineal activities in vitro experiments, hypothalamus, anterior hypophysis, paper electrophoresis.
D000818:Animals; D001681:Biological Assay; D002540:Cerebral Cortex / Q000032:analysis; D002850:Chromatography, Gel; D004590:Electrophoresis, Paper; D005260:Female; D006062:Gonadotropins / Q000378:metabolism; D007031:Hypothalamus / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008722:Methods; D051379:Mice; D010870:Pineal Gland; D010902:Pituitary Gland / Q000502:physiology*; D010903:Pituitary Gland, Anterior / Q000502:physiology*; D011863:Radioimmunoassay; D051381:Rats; D012756:Sheep; D013347:Subcellular Fractions; D014020:Tissue Extracts / Q000032:analysis*
D006062:Gonadotropins; D014020:Tissue Extracts