Effect of pituitary gonadotrophins on tritiated thymidine uptake by rat ovary. An autoradiographic study.
The effect of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) on the follicular growth in the ovary of the hypophysectomized rat was investigated using autoradiography. The numbers of DNA-synthesizing nuclei in the granulosa cell were measured by autoradiography after flashlabelling with tritiated (3-H) thymidine. The frequency of 3-H-thymidine labelled nuclei in the granulosa cell enhanced in the presence of FSH. In contrast, LH had no significant effect on thymidine uptake. The result suggests that FSH stimulates follicle cell division, whereas LH does not.
D000818:Animals; D001345:Autoradiography; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006065:Gonadotropins, Pituitary / Q000494:pharmacology*; D007016:Hypophysectomy; D007986:Luteinizing Hormone / Q000494:pharmacology; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium
D006065:Gonadotropins, Pituitary; D014316:Tritium; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D004247:DNA; D013936:Thymidine