Spectrofluorometric and spectrodensitometric determination of chloroquine in plasma and urine.
Chloroquine in a concentration range from 5 ng to 2 mug per ml of biological fluid can be determined in plasma and urine by the spectrofluorometric methods. The spectrodensitometric method is less sensitive and should be used when other drugs are interfering with the chloroquine determination in solution. After extraction from urine and separation from interfering drugs and metabolites by TL-chromatography chloroquine can be determined by spectrodensitometry in a minimum concentration of 5 mug per ml of urine. Chloroquine should be determined in plasma and urine of patients suffering from malaria with an impaired renal function. Whether the fluorometric determination of chloroquine should be used in field investigations will depend on the problem to be investigated and the regional facilities.
D002738:Chloroquine / Q000032:analysis* / Q000097:blood / Q000652:urine; D003720:Densitometry / Q000379:methods; D006801:Humans; D007674:Kidney Diseases / Q000378:metabolism; D008288:Malaria / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods
D002738:Chloroquine