Comparison of four different methods for the recovery of dotriacontane-16,17-14C from small animal respiratory tissue.
The efficiency and accuracy of four different methods of tissue preparation for the recovery of dotriacontane-16,17-14C (DOT-16,17-14C) from rat respiratory tissue were compared by means of liquid scintillation counting. A DOT-16,17-14C standard solution was placed on the respiratory epithelium of the nasal apex, larynx, trachea and main bronchi. Combustion of the lyophilized organs in a Packard sample oxidizer revealed the highest recovery and accuracy (93--100%), sample oxidizing by combustion without lyophilization the lowest recovery and accuracy (73--92%). Tissue solubilization by the commercially available tissue solubilizer TS-1 revealed a better recovery and accuracy (89--95%) than tissue maceration by boiling in methanolic KOH and toluene extraction (74--91%). Depending on the laboratory equipment, lyophilization or tissue solubilization is preferred; maceration and toluene extraction as well as combustion without freeze-drying are disregarded for further investigations because of the cumbersome procedure as well as the low recovery and accuracy, respectively.
D000818:Animals; D001842:Bone and Bones / Q000032:analysis; D002250:Carbon Radioisotopes; D002356:Cartilage / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005260:Female; D005612:Freeze Drying; D006838:Hydrocarbons / Q000032:analysis* / Q000378:metabolism; D007817:Laryngeal Cartilages / Q000032:analysis; D007830:Larynx / Q000032:analysis; D008168:Lung / Q000032:analysis; D008297:Male; D009666:Nose / Q000032:analysis; D009929:Organ Size; D051381:Rats; D012137:Respiratory System / Q000032:analysis* / Q000378:metabolism; D012907:Smoking; D014132:Trachea / Q000032:analysis
D002250:Carbon Radioisotopes; D006838:Hydrocarbons