Inhibitory effects of synthetic rat C-peptide 1 on insulin secretion in the isolated perfused rat pancreas.
The effects of synthetic rat C-peptide 1 on insulin secretion from the isolated perfused rat pancreas was studied. The perfusion technique was according to the procedure described by Grodsky et al (1963) with a few modifications. After about 20 min of stabilization, the pancreas was perfused for 40 min with synthetic rat C-peptide 1 (100 ng/ml), during which (at 20 min) glucose concentration was switched from 2.7 to 16.6 mM. Insulin response to glucose (16.6 mM) was significantly decreased by the addition of the rat C-peptide 1 in advance when compared with those not pretreated with the C-peptide. In other experiments, the pancreas was exposed to glucose infusion (16.6 mM) for 60 min. 20 min after the start of glucose infusion, the C-peptide (100 ng/ml) was interposed for 20 min. The insulin secretion was reduced by the addition of the C-peptide. It is postulated that the C-peptide may have a role in the regulatory mechanism of the insulin secretion.
D000818:Animals; D003864:Depression, Chemical; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D010477:Perfusion; D011384:Proinsulin; D051381:Rats
D007328:Insulin; D010455:Peptides; D011384:Proinsulin; D005947:Glucose