Dipotassium tetramethyl osmate: a stain for electron microscopy.
Methanol solutions of dipotassium tetramethyl osmate (DTMO) have been found to be useful as general stains in electron microscopic studies of plant and fungal ultrastructure. The stain solutions are easy to prepare, stable when anhydrous and convenient to use. Although generally similar in staining to lead citrate stains, some elements of cell ultrastructure appear different with dipotassium tetramethyl osmate staining, particularly the outer cell walls of fungi. Indications of specific precipitate-producing reactions in cell storage areas are observed.
D002473:Cell Wall / Q000648:ultrastructure; D002951:Citrates; D005069:Evaluation Studies as Topic; D006651:Histocytochemistry; D008854:Microscopy, Electron / Q000379:methods*; D009235:Myxomycetes / Q000648:ultrastructure*; D009992:Osmium; D010944:Plants / Q000648:ultrastructure
D002951:Citrates; D009992:Osmium