Requirement for ribosome-releasing factor for the release of ribosomes at the termination codon.
With the use of 3H-labeled R 17 amB2 phage RNA having an UAG codon at the seventh triplet of the coat cistron, release of the RNA from ribosomes at the termination codon was studied. The ribosome-releasing factor previously described was shown to stimulate the process of mRNA release at the termination factor (RF-1). GTP was required for this process and guanosine 5'-(beta,gamma-methylene)triphosphate could not replace GTP. No apparent change of size of R 17 RNA was observed during the release of the R 17 RNA from the ribosomes. The ribosome-releasing factor is distinct from the known termination codon-specific factor such as RF-1.
D000596:Amino Acids; D001216:Asparagine / Q000494:pharmacology; D003062:Codon / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D010445:Peptide Elongation Factors; D010448:Peptide Initiation Factors; D010454:Peptide Termination Factors; D011132:Polyribosomes / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D000596:Amino Acids; D003062:Codon; D010445:Peptide Elongation Factors; D010448:Peptide Initiation Factors; D010454:Peptide Termination Factors; D012333:RNA, Messenger; C011265:guanosine 5'-(beta,gamma-methylene)triphosphate; D011691:Puromycin; D001216:Asparagine; D006160:Guanosine Triphosphate; D012343:RNA, Transfer