Some characteristics of monocarboxylic acid transfer across the cell membrane of epithelial cells from rat small intestine.
1. The translocation of monovalent organic anions (pyruvate, propionate,  acetate and butyrate) across the cell membrane of isolated epithelial cells from rat small intestine was studied by measuring competitive inhibition kinetics, exchange diffusion and temperature dependence of the efflux rate. A possible function of a monocarboxylate carrier in intestine will be discussed. 2. Earlier studies on the inhibition of pyruvate transport of fatty acids were extended to propionate and found to show the same characteristics. The kinetics, however, appeared to be more complex by the contribution of several diffusion pathways for propionate. 3. The mechanism of countertransport was most compatible with an "accelerated exchange diffusion" and could be studied at both sides of the membrane. This exchange diffusion exhibited saturation kinetics. It is proposed that different monocarboxylate anions may have different affinities for a common carrier. 4. Temperature dependence of the efflux of pyruvate and propionate was studied. Arrhenius plots obtained were not found to be linear between 0 and 5 degrees C. Between 5 and 15 degrees C activation energies for pyruvate and propionate efflux rates were found to be 19.6 and 12.6 kcal/mol, respectively.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D001693:Biological Transport, Active; D002087:Butyrates / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism* / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D011422:Propionates / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D013696:Temperature
D000085:Acetates; D002087:Butyrates; D002264:Carboxylic Acids; D011422:Propionates; D011773:Pyruvates