Quantitation of the ABO system antigens by means of isoprecipitating antisera.
A quantitative method for the determination of blood group specific substances A and B on red blood cells was developed, using them to absorb isoprecipitating antisera anti-A and anti-B, followed by estimation of the residual antibody by means of a counterimmunoelectrophoretic technique with standard solutions of A and B substances prepared from saliva. The method was tested on a population of A2B donors. The mean amount of A substance in A2B RBC was 131 X 10(-12) +/- 217 X 10(-12) u/cell in the population of 95 donors. The A substance thus determined agreed significantly with values obtained by classical haemagglutinating titration. The mean amount of B substance in A2B RBC was 222 X 10(-12) +/- 178 X 10(-12) m/cell. The amounts of both the A and B substances were not correlated on the same RBC. Several peaks of the distribution curve of both substances confirmed the non-homogeneity of the populations, there being a wide standard deviation presumably due to differences in the antigen content of different subgroups.
D000017:ABO Blood-Group System; D011232:Chemical Precipitation; D003378:Counterimmunoelectrophoresis; D005260:Female; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D008297:Male; D011234:Precipitins / Q000302:isolation & purification
D000017:ABO Blood-Group System; D007518:Isoantibodies; D011234:Precipitins