Further studies on the lens cell-free system. In vitro synthesis of the non-crystallin lens proteins.
1. The lens cell-free system synthesizes in addition to the crystallins, polypeptides which co-electrophorese with lens plasma membrane protein components. 2. Isolated lens polysomes can be translated in a heterologous cell-free system. They code for both the crystallins and membrane-protein-like components. 3. If messengers isolated from these polysomes by affinity chromatography on oligo-(dT)-cellulose are added to a heterologous cell-free system, only lens proteins of lower molecular weight are synthesized. 4. Different ionic conditions are required for optimal translation of different lens messengers.
D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D003459:Crystallins / Q000096:biosynthesis*; D007908:Lens, Crystalline / Q000378:metabolism*; D008970:Molecular Weight; D011132:Polyribosomes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D003459:Crystallins; D012333:RNA, Messenger