[Cryopreservation of encysted Toxoplasms in liquid nitrogen (author's transl)].
USing an automatically controlled freezing system (cooling rate between 1,4 and 2,7 degrees C/min) with the addition of cyroprotectants (Dimethylsulfoxyd, Polivinyl-Pyrrolidon), we have been successful in maintaining encysted toxoplasms infectious for a minimum of 18 months.
D000818:Animals; D001331:Automation; D001921:Brain / Q000469:parasitology; D002470:Cell Survival; D005615:Freezing; D051379:Mice; D009584:Nitrogen; D011309:Preservation, Biological / Q000379:methods*; D013997:Time Factors; D014122:Toxoplasma / Q000254:growth & development* / Q000472:pathogenicity
D009584:Nitrogen
Konservierung von Toxoplasmem im Zystenstadium in flüssigem Stickstoff