[Basic peptides from bee venom, I: isolation, reduction and reoxidation of apamin and MCD-peptide (author's transl)].
A simple isolation of melittin, apamin and MCD-peptide from bee venom is described. The peptides were separated using Sephadex G-50 and Biogel P-4 followed by preparative column chromatography using silicagel. Apamin and MCD-peptide were converted to the tetra-S-sulfonate derivatives by oxidative sulfitolysis. After reduction of these derivatives by mercaptoethanol followed by cautious air-reoxidation, the disulfide bridges can be reconstituted. The biological activities of the reoxidation products are 50 to 60% for apamin and 70 to 80% for MCD-peptide.
D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000032:analysis*; D002850:Chromatography, Gel; D004220:Disulfides / Q000032:analysis; D008555:Melitten / Q000302:isolation & purification; D010084:Oxidation-Reduction; D010455:Peptides / Q000302:isolation & purification; D011485:Protein Binding; D011487:Protein Conformation; D014688:Venoms / Q000032:analysis*
D004220:Disulfides; D010455:Peptides; D014688:Venoms; D008555:Melitten
Basische Peptide des Bienengifts, I. isolierung, Reduktion und Reoxidation von Apamin und MCD-Peptid