An example of lyophilized protein-based materials not simulating patient sera.
Use of reconstituted lyophilized protein-based materials in the clinical laboratory is partly based on the assumption that these materials adequately simulate patients' sera. We examined several of these materials and found that certain ones do not have the same adsorbancies at 340 and 380 nm as do most sera. The implication of this is examined with respect to glucose determination by the hexokinase method on a dual-wave-length blank-subtraction instrument.
D001322:Autoanalysis; D001786:Blood Glucose; D001798:Blood Proteins / Q000032:analysis*; D005612:Freeze Drying; D006801:Humans; D007565:Jaundice / Q000097:blood; D007753:Laboratories / Q000592:standards; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D001786:Blood Glucose; D001798:Blood Proteins