RNA polymerase activity of Amsacta moorei entomopox virions.
Optimum conditions for in vitro RNA synthesis by the entomopoxvirus from Amsacta moorei, except for a temperature optimum of 26 degrees, were similar to those reported for vaccinia. Incorporation of 3H-ATP in the presence of CTP, GTP and UTP was significantly inhibited by actinomycin D; incorporation of 3H-ATP alone was not. The products formed by incorporation of 3H-ATP alone or with the three other nucleotides contained polyadenylic acid sequences. The sedimentation coefficient of the 3H-ATP-labeled product formed in the presence of CTP, GTP and UTP was reduced from 8-23S to 3-5S after RNase treatment. The product formed from 3H-ATP alone had an apparent sedimentation value of 3-5S.
D002474:Cell-Free System; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D007304:Insect Viruses / Q000201:enzymology*; D009711:Nucleotides / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D013696:Temperature
D009711:Nucleotides; D012367:RNA, Viral; D003609:Dactinomycin; D012321:DNA-Directed RNA Polymerases