[Radioimmunoassay of n-butylbiguanide in human serum].
A radioimmuno assay for butylbiguanide in human serum is described. The detection limit is 1.5 ng per assay tube, the intra assay variance 3 percent, and the inter assay variability 12 percent. Antibodies to the biguanide group were obtained by immunizing rabbits with p-carboxy-phenethyl-biguanide coupled to bovine serum albumin. The affinity of the antibodies for tyrosine, urea, guanidine, butylbiguanide, phenethyl-biguanide, and p-hydroxy-phenethyl-biguanide is shown by competitive binding studies. The recovery rate is 100 percent.
D000818:Animals; D000936:Antigen-Antibody Complex; D001645:Biguanides / Q000097:blood*; D001667:Binding, Competitive; D002417:Cattle; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera; D007457:Iodine Radioisotopes; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014443:Tyrosine / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D000936:Antigen-Antibody Complex; D001645:Biguanides; D006146:Guanidines; D007106:Immune Sera; D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine; D014443:Tyrosine; D014508:Urea
Radioimmunologische Bestimmung von n-Butylbiguanid im menschlichen Serum