Shifts in ATP synthesis during preimplantation stages of mouse embryos.
The actual and potential activities of the cyochrome system were studied in cleavage-stage mouse embroys. Activities were determined by assaying embroys for total ATP and the rates of [32-P]ATP synthesis both before and after their incubation in medium supplemented either with an energy coupling site inhibitor (antimycin, amytal or cyanide) or with the FADH-linked substrate, succinate. The data indicate that there are three major shifts in the mode of ATP production during preimplantation stages: the first, between the two-cell and late four-cell stages; the second, between the eight-celland late morula stages; and the third, between the late morula and late blastocyst stages. These data are discussed in relation to studies on the energy metabolism of cleavage and blastocyst stage mouse embryos.
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000654:Amobarbital / Q000494:pharmacology; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001665:Binding Sites; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D003486:Cyanides / Q000494:pharmacology; D047108:Embryonic Development; D005260:Female; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal; D013386:Succinates / Q000494:pharmacology
D003486:Cyanides; D013386:Succinates; D000968:Antimycin A; D000255:Adenosine Triphosphate; D000654:Amobarbital