Structure of nuclear pre-mRNA. VI. "Reversed repeats" in animal DNA and their hybridization with double-stranded regions of pre-mRNA.
About 2% of mouse DNA reassociates at extremely low Cot values (10-7-10-6 mole-liter-1-sec). This DNA fraction was isolated with the aid of nuclease S1 and purified by chromatography on hydroxyapatite. The study of this fraction suggests that it has a structure of hairpin type. The DNA of the hairpins can hybridize with sequences forming the double-stranded regions in pre-mRNA. Probably at least some of the DNA of the hairpins, described as "reversed repeating sequences" of DNA, is transcribed with the formation of structures of hairpin type in pre-mRNA.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002467:Cell Nucleus / Q000032:analysis*; D002845:Chromatography; D004247:DNA; D003851:Deoxyribonucleases; D005260:Female; D005557:Formaldehyde; D006882:Hydroxyapatites; D007700:Kinetics; D007739:L Cells / Q000032:analysis; D051379:Mice; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D014158:Transcription, Genetic
D006882:Hydroxyapatites; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D005557:Formaldehyde; D004247:DNA; D003851:Deoxyribonucleases