High-pressure liquid chromatographic determination of sulfamethazine residue in bovine tissue.
A sensitive high-pressure liquid chromatographic assay procedure is described for residues of sulfamethazine in bovine kidney, liver, muscle, and fat tissue at a sensitivity of 0.04 ppm. Studies were conducted using sulfamethazine-negative bovine tissue to ensure that the peak eluting at the indicated retention volume was sulfamethazine and that the recoveries from spiked tissues were consistent. Data are presented showing the levels of sulfamethazine in the tissue of 12 treated animals found by this technique. The method is more specific than the frequently used colorimetric methods and does not require the tedious separations involved in TLC assays.
D000273:Adipose Tissue / Q000032:analysis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008722:Methods; D009132:Muscles / Q000032:analysis; D013418:Sulfamethazine / Q000032:analysis*
D013418:Sulfamethazine