Accumulation and excretion of neutral lipids in the metacercaria of Leucochloridiomorpha constantiae (Trematoda) maintained in vitro.
Histochemical and thin-layer chromatographic analyses were made on neutral lipids in Leucochloridiomorpha constantiae (Trematoda) metacercariae incubated in non-nutrient Locke's solution at 37.5 or 42 C for 24 hr. As determined by Oil Red O staining, lipid droplets accumulate mainly in the intestine of incubated worms. Attempts to distinguish specific neutral lipid fraction of metacercariae obtained directly from snails is free sterol. During incubation, metacercariae accumulate triglycerides and to a lesser extent cholesterol esters, and excrete free sterol into the medium.
D000818:Animals; D002432:Cecum / Q000378:metabolism; D006651:Histocytochemistry; D050356:Lipid Metabolism; D013261:Sterols / Q000378:metabolism; D014200:Trematoda / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D013261:Sterols; D014280:Triglycerides