Transport-associated phosphorylation of 2-deoxy-D-glucose in Saccharomyces fragilis.
2-Deoxy-D-glucose transport and metabolism was studied in Saccharomyces fragilis. Inside the cells four phosphorylated and three non-phosphorylated derivatives were found and identified. Accumulation of phosphorylated 2-deoxyglucose derivatives was balanced by a concomitant decrease of cellular ATP, orthophosphate and polyphosphates. The free sugar was concentrated against a concentration gradient, contradiciting facilitated diffusion. Pulse labeling experiments revealed transport-associated phosphorylation. Theoretical considerations and analysis of the effects of iodoacetate showed that an intracellular hexokinase activity was not involved in 2-deoxyglucose phosphorylation, although this sugar is a good substrate for the enzyme in in vitro experiments.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000332:Aerobiosis; D000693:Anaerobiosis; D001693:Biological Transport, Active; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D006600:Hexosephosphates / Q000096:biosynthesis; D007700:Kinetics; D008433:Mathematics; D012440:Saccharomyces / Q000378:metabolism*
D003837:Deoxy Sugars; D006600:Hexosephosphates; D000255:Adenosine Triphosphate; D003847:Deoxyglucose