[Value of determination of mononuclear blood cell proliferation in vitro in the diagnosis of lymphosarcoma].
The authors describe mitotic activity in non-stimulated cultures of mononuclear cells obtained from the peripheral blood of patients with lymphosarcoma. The investigations were carried out on 3 patients in stage 3rd and 4th of this disease and 5 patients in the stage of sarcomatous-cell leukaemia. During a period of 24-48 hours after setting up of culture a rise was observed in the number of blast cells and appearance of up to 10% of mitotic figures. This observation may be useful for differentiation of sarcomatous-cell leukaemia from chronic lymphocytic leukaemia in which cells obtained from the peripheral blood and cultured in vitro without addition of mitogen do not demonstrate mitotic activity.
D000293:Adolescent; D000328:Adult; D000368:Aged; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood / Q000175:diagnosis; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D013997:Time Factors
Wartość badania proliferacji jednojadrzastych komórek krwi in vitro dla rozpoznawania miesaka limfatycznego