Microsomal N-hydroxylation of dibenzylamine.
1. The properties of the rabbit liver microsomal enzyme system(s) catalysing the formation of N,N-dibenzylhydroxylamine as the major metabolite of dibenzylamine have been investigated. 2. The system consists of NADPH- and NADH-dependent components which are differentiated by their different pH optima and sensitivity towards cyanide. 3. The effect of various metabolic inhibitors on the N-oxidation process in vitro are investigated. 4. The N-oxidation of the parent amine was inhibited by CO, SKF 525-A, and  inhibitors known to interact with microsomal cytochrome P-450. Phenobarbitone pre-treatment stimulates further metabolism of the hydroxylamine.
D000818:Animals; D001593:Benzyl Compounds / Q000378:metabolism*; D002415:Cats; D002645:Chickens; D006224:Cricetinae; D005260:Female; D006168:Guinea Pigs; D006898:Hydroxylamines / Q000378:metabolism*; D006900:Hydroxylation; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D001593:Benzyl Compounds; D006898:Hydroxylamines; D010088:Oxidoreductases