Mutagenesis in cultured human diploid cells. III. Induction of 8-azaguanine-resistant mutations by furylfuramide.
Trans-2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (furylfuramide: FF or AF2) was tested for ability to induce 8-azaguanine (8AG) resistant mutations in cultured human diploid cells. FF had a relatively severe cytotoxic effect on the cells. From the concentration-survival curve, the D0 value for 2-h treatment with FF was estimated to be 11 mug/ml. When cells were treated with FF at various concentrations for 2 h, cultured in normal medium for 48 h, and then selected in medium containing 8AG at 30 mug/ml, the induced mutation frequency increased gradually with increase in concentration of FF. When cells were treated with FF at 10 mug/ml for 2 h, cultured in normal medium for various periods of mutation expression time, and selected with 8AG at 30 mug/ml, the highest induced mutation frequency was obtained with 48 h of mutation expression time. Microscopic examination of the numbers of cells in colonies indicated that the total number of cells increased by half during this mutation expression time of 48 h.
D000178:Acrylamides / Q000494:pharmacology; D001375:Azaguanine / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D004171:Diploidy; D004622:Embryo, Mammalian; D005520:Food Preservatives / Q000494:pharmacology; D005787:Gene Frequency; D006801:Humans; D008168:Lung; D009153:Mutagens; D009581:Nitrofurans / Q000494:pharmacology*; D013997:Time Factors
D000178:Acrylamides; D005520:Food Preservatives; D009153:Mutagens; D009581:Nitrofurans; D001375:Azaguanine