Spontaneous release of soluble HL-A antigens from platelets during conservation.
Experiments with the aim of studying the solubilisation of HL-A antigens from blood platelets by methods which do not involve any biologically active processes (moderate, discontinuous agitation of a low concentration of platelets suspended in a saline medium, in the presence of an antiseptic; supernatants collected at frequent intervals) have shown that platelets release membrane proteins, including HL-A antigens, spontaneously. Optimal conditions for the treatment of membrane proteins have been perfected. The great stability of HL-A antigens under these conditions permits prolonged treatment. The products extracted are soluble and extremely complex. The molecular weight of the HL-A antigens is between 40,000 and 70,000.
D001792:Blood Platelets / Q000276:immunology* / Q000502:physiology; D002462:Cell Membrane / Q000276:immunology; D003168:Complement Fixation Tests; D006680:HLA Antigens / Q000032:analysis*; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D008970:Molecular Weight; D012995:Solubility
D006680:HLA Antigens; D006649:Histocompatibility Antigens