Run-off ribosomes in liver, spleen and pancreas of the rat.
Free polyribosomes, isolated from liver, spleen and pancreas of the rat, were suspended in a medium at 0.5 mM Mg2+ and analyzed in the analytical ultracentrifuge. The percentage of run-off ribosomes, distinguised by a sedimentation coefficient below 77S was calculated from centrifugal experiments. The amount of run-off ribosomes differed in the various tissues of the rat but was not influenced by fasting overnight.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D005215:Fasting; D008099:Liver / Q000648:ultrastructure*; D008274:Magnesium; D009928:Organ Specificity; D010179:Pancreas / Q000648:ultrastructure*; D011132:Polyribosomes / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012260:Ribonucleases; D012270:Ribosomes / Q000648:ultrastructure*; D013154:Spleen / Q000648:ultrastructure*
D012333:RNA, Messenger; D012260:Ribonucleases; D008274:Magnesium