Microchromatography of hemoglobins. V. Thin-layer chromatography of some hemoglobins on CM-cellulose.
By means of thin-layer ion-exchange chromatography, human hemoglobins A, S, and C in various combinations can be distinctly separated. Also, hemoglobin F can be separated from the A, S, and C components of cord blood samples from infants and in adult blood samples with a relatively high percentage of F (15% or more). The procedure uses CM-cellulose and solutions of Tris-HCl, NaCl, and KCN. A 0.3-mg sample in 5-10 mul of solvent is required. Development can be completed in 30-40 min.
D000367:Age Factors; D002266:Carboxymethylcellulose Sodium; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D006454:Hemoglobins / Q000032:analysis* / Q000145:classification; D006455:Hemoglobins, Abnormal / Q000032:analysis; D006801:Humans; D008832:Microchemistry; D012997:Solvents
D006454:Hemoglobins; D006455:Hemoglobins, Abnormal; D012997:Solvents; D002266:Carboxymethylcellulose Sodium