The effect of "ouabain" on the ultrastructure of cerebral arterioles and surrounding tissue, studied by a cannulation of a cerebral artery.
A technique for cannulation of a parietal branch of the middle cerebral artery is described by which high but local concentrations of substances can be achieved in cortical vessels. Using this technique it was shown that ouabain, a specific inhibitor of the Na+-K+-ATPase enzyme system, can produce alterations in the blood brain barrier (BBB) permeability as seen by the passage of Evans Blue into cortical tissue. Electron microscopy revealed changes in the endothelium of cerebral arterioles ranging from an increase in the number of vesicles and vacuoles to complete breakdown of cytoplasm and membranes. Swelling of the peri-arteriolar end feet of protoplasmic astroglia and of dendrites was characteristic of tissue surrounding affected arterioles. Swollen fibrous astrocytes, oligodendrocytes and microglia were not seen even in areas of vasogenic edema. These results are discussed in terms of current ideas of the BBB and astroglial function.
D000818:Animals; D001253:Astrocytes / Q000648:ultrastructure; D001812:Blood-Brain Barrier / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000648:ultrastructure; D002199:Capillary Permeability / Q000187:drug effects; D002415:Cats; D002536:Cerebral Arteries / Q000187:drug effects*; D003712:Dendrites / Q000648:ultrastructure; D004727:Endothelium / Q000648:ultrastructure; D008833:Microcirculation / Q000187:drug effects / Q000648:ultrastructure; D008933:Mitochondrial Swelling; D010042:Ouabain / Q000494:pharmacology*; D013572:Synaptic Vesicles / Q000648:ultrastructure
D010042:Ouabain