[Post-mortem decay of rat skeletal muscle polysomes].
Post-mortem changes of cytoplasmic RNP particles from rat skeletal muscles are studied using sucrose density gradient. The polysome destruction is found to beign within 1-2 hours after the animal death. However, a part of polysomes turned to be stable at least within 48-60 hours after the death. These polysomes incorporated 14C-leucine into acid insoluble fraction in cell-free system and were decomposited by RNase treatment (10 mkg/ml). The data suggest that within 24-48 hours of the after-death destruction the decay begins to free monoribosomes while there are still active polysomes in muscles.
D000818:Animals; D002499:Centrifugation, Density Gradient; D007930:Leucine / Q000378:metabolism; D009132:Muscles / Q000378:metabolism / Q000648:ultrastructure*; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism*; D011180:Postmortem Changes; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology; D012270:Ribosomes / Q000378:metabolism; D013997:Time Factors
D012260:Ribonucleases; D007930:Leucine
Izuchenie posmertnogo raspada polisom skeletnykh myshts krys