[Determination of the number of cells with multiple nucleoli in histological sections (author's transl)].
A method has been described for correction of a bias, when cells with more than one nucleolus are counted by counting their nucleoli. The true numbers of cells and nucleoli per cell can be estimated by a system of linear equations, with coefficients depending on the thickness of the histological specimen, the average nuclear diameter and the probabilities of nuclear slices, which one gets by cutting a cell nucleus with a certain number of nucleoli. The presuppositions for this method have been discussed with respect to different practical situations. Uncorrected and corrected numbers of motoneurons in the nucl. n. oculomotorii of Tupaia belangeri have been compared.
D000818:Animals; D002452:Cell Count / Q000379:methods*; D002466:Cell Nucleolus; D008433:Mathematics; D008954:Models, Biological; D009046:Motor Neurons / Q000648:ultrastructure; D009802:Oculomotor Nerve / Q000648:ultrastructure; D012788:Shrews / Q000033:anatomy & histology
Bestimmung der Anzahl der Zellen mit mehr als einem Nucleolus im histologischen Schnittpriäparat