[Effect of the composition of the medium and magnesium and calcium ions on the germination of spores of Thermoactinomyces vulgaris].
The germination of the spores of Thermoactinomyces vulgaris formed on a complex medium is stimulated by suspending them in solutions containing Mg2+ and Ca2+ ions. The stimulation is not the result of the initiation of the spores in the presence of the ions since the experiments were carried out at a temperature of 20 degrees C at which the initiation did not virtually take place. The ions of Na+ and K+ have almost no effect on the germination of the spores. The fraction of the resting spores of Thermoactinomyces vulgaris depends on the composition of the growth medium, especially on its amino acid composition. The addition of Mg2+ and Ca2+ ions to a minimal synthetic growth medium stimulates the growth of the cultures and decreases the dormancy of the spores. The spores formed on the synthetic medium are less thermostable than the spores formed on the complex medium. Thermostability of the spores increases upon the addition of Mg2+ to the synthetic medium. Spore suspensions obtained on the synthetic medium with Mg2+ or Ca2+ are initiated more completely than spore suspensions obtained on the complex medium.
D000596:Amino Acids; D002118:Calcium / Q000494:pharmacology*; D003470:Culture Media; D008274:Magnesium / Q000494:pharmacology*; D008848:Micromonosporaceae / Q000187:drug effects / Q000254:growth & development*; D013171:Spores, Bacterial / Q000187:drug effects / Q000254:growth & development; D013696:Temperature
D000596:Amino Acids; D003470:Culture Media; D008274:Magnesium; D002118:Calcium
Prorastanie spor Thermoactinomyces vulgaris: znachenie sostava sredy, rol' Mg2+ i Ca2+