title: Cellular and free lipopolysaccharides of some species of Neisseria.
abstract: Cultures of eight non-pathogenic species of Neisseria grown in simple defined media released lipopolysaccharide (free lipopolysaccharide) by a process distinct from cellular autolysis. Analyses of the pure cellular and free lipopolysaccharides obtained from six species of Neisseria revealed that they were remarkably similar and were devoid of detectable O-antigen side chains. Three distinct types of core-oligosaccharides were demonstrated. Type I core-oligosaccharide was a branched structure of alpha-D-glucopyranosyl units (7 mol) terminated by a reducing end group of 3-deoxy-D-manno-octulosonic acid. Type II core-oligosaccharide contained D-glucose, 2-deoxy-2-amino-D-glucose, L-rhamnose, L-glycero-D-manno-heptose, 3-deoxy-D-manno-octulosonic acid, phosphate, and ethanolamine in a molar ratio of 3:2:1:1:1:1:1. Type III coreoligosaccharide was composed of D-glucose, L-glycero-D-manno-heptose, 3-deoxy-D-manno-octulosonic acid, and phosphate in a molar ratio of 3:3:1:1. Lipopolysaccharides of N. caviae and N. sicca contained type I core-oligosaccharides exclusively, while those of N. flava and N. perflava contained only type II core-oligosaccharide. Cellular lipopolysaccharide from N. cinerea contained core-oligosaccharides of types I and II in a ratio of 27:73, while the analogous preparation from N. flavescens contained core-oligosaccharide types II and III in a ratio of 21:4. Free lipopolysaccharides from these two organisms contained only one type of coreoligosaccharide. Lipid A components of all the lipopolysaccharide preparations were very similar being composed of about 25% by weight of dodecanoic acid, 3-hydroxy-dodecanoic acid, and 3-hydroxy-tetradecanoic acid.
mesh_terms: D000606:Amino Sugars / Q000032:analysis; D004731:Endotoxins / Q000032:analysis*; D004983:Ethanolamines / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D006539:Heptoses / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D009343:Neisseria / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D012210:Rhamnose / Q000032:analysis; D013045:Species Specificity
chemical_list: D000606:Amino Sugars; D004731:Endotoxins; D004983:Ethanolamines; D005227:Fatty Acids; D005960:Glucosides; D006539:Heptoses; D008055:Lipids; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D012210:Rhamnose