Genetic control and intracellular localization of glutamate oxaloacetic transaminase in maize.
Glutamate oxaloacetic transaminase (L-aspertate: 2-oxoglutarate aminotransferase, E.C. 2.6.1.1; GOT) was found to occur in five distinct electrophoretic forms in different tissue extracts from a number of highly inbred strains of Zea mays L. No major qualitative differences were detected in the various tissues examined, and the isozyme patterns did not undergo changes during temporal development of any given inbred strain. Cell fractionation studies showed one isozyme to be associated with the mitochondria (mGOT), another to be exclusively associated with the soluble fraction (sGOT), and a third to be associated with the glyoxysomes (gGOT). The glyoxysomal form occurs as two electrophoretically distinct variants which exist in different inbred strains of maize. The gGOT variants are under the control of two codiminant alleles (Got1A and Got1B) at the Got1 locus (isozyme5, gGOT). The genetic data and gene dosage effects suggest that GOT in maize is functionally a dimer.
D001219:Aspartate Aminotransferases / Q000378:metabolism*; D003433:Crosses, Genetic; D014644:Genetic Variation; D006579:Heterozygote; D006720:Homozygote; D007527:Isoenzymes / Q000378:metabolism*; D010641:Phenotype; D010944:Plants / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology; D003313:Zea mays / Q000201:enzymology
D007527:Isoenzymes; D001219:Aspartate Aminotransferases