title: Double labeling with [3H]thymidine and [125I]iododeoxyuridine as a method for determining the fate of injected DNA and cells in vivo.
abstract: Mice were injected intravenously and intraperitoneally with preparations of intestinal nucleoprotein, spleen nuclei, mouse thymus cells, or human kidney T cells whose DNA had been labeled with both [3H]thymidine (TdR) and [125I]-iododeoxyuridine (IUdR). Since free TdR is reutilized more efficiently than free IUdR produced by enzymic hydrolysis of the exogenous DNA, the ratio of [3H]TdR/[125I]IUdR in the DNA fraction of the tissues of the recipient mice provides a measure of the amount of intact exogenous DNA in the tissue. In most instances, the doubly labeled exogenous DNA was almost completely hydrolyzed within 1 day injection, but survival of the DNA from whole cells could be demonstrated in some cases.
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002460:Cell Line; D002467:Cell Nucleus; D004247:DNA / Q000378:metabolism*; D005260:Female; D007065:Idoxuridine / Q000378:metabolism*; D007457:Iodine Radioisotopes; D051379:Mice; D008815:Mice, Inbred Strains; D009698:Nucleoproteins / Q000378:metabolism; D011849:Radioactive Tracers; D013154:Spleen / Q000648:ultrastructure; D013936:Thymidine / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology; D014316:Tritium
chemical_list: D007457:Iodine Radioisotopes; D009698:Nucleoproteins; D011849:Radioactive Tracers; D014316:Tritium; D004247:DNA; D007065:Idoxuridine; D013936:Thymidine