Preparation of peritoneal cell metaphases of rats, mice and chinese hamsters after mitogenic stimulation with magnesium sulfate and/or aluminum hydroxide.
A new cytogenetic system in vivo is presented using peritoneal cells, collected from unanesthetized rats, mice and Chinese hamsters, 48 h after an i.p. injection of a mitogen. The test is useful for periodic monitoring of chromosome aberration rates in the investigation of such problems as aging and chronic effects of mutagens and carcinogens. Among the 2 mitogens tested, aluminum hydroxyde was more efficient than magnesium sulfate in all 3 species. The rat is considered to be the most suitable animal for this test, in view of its easy handling, high cell yield and favourable response to mitogens. The spontaneous chromosome aberration rate in this animal was found to be comparable with that of bone-marrow cells.
D000536:Aluminum Hydroxide / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D002452:Cell Count; D002869:Chromosome Aberrations; D006224:Cricetinae; D007274:Injections, Intraperitoneal; D007621:Karyotyping / Q000379:methods*; D008278:Magnesium Sulfate / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D008934:Mitogens; D008938:Mitosis / Q000187:drug effects*; D008940:Mitotic Index; D051381:Rats
D008934:Mitogens; D000536:Aluminum Hydroxide; D008278:Magnesium Sulfate