Galactose oxidase-induced blastogenesis of human lymphocytes and the effect of macrophages on the reaction.
Treatment of human lymphocytes with neuraminidase and galactose oxidase induced extensive blastogenesis. A less pronounced effect was observed after treatment of the cells with galactose oxidase alone. Macrophage-depleted human lymphocytes had a markedly reduced blastogenic response after treatment with neuraminidase and galactose oxidase. Incubation of the purified lymphocytes on macrophage monolayers markedly enhanced their response to neuraminidase and galactose oxidase. Furthermore, a stimulation of thymidine incorporation into purified untreated lymphocytes was noted after incubation of the cells on neuraminidase and galactose oxidase-treated macrophage monolayers.
D000429:Alcohol Oxidoreductases / Q000494:pharmacology*; D002478:Cells, Cultured; D005692:Galactose Oxidase / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D009439:Neuraminidase / Q000494:pharmacology
D000429:Alcohol Oxidoreductases; D005692:Galactose Oxidase; D009439:Neuraminidase