Isolation and characterization of a sulfated glycoprotein from rabbit uterus.
Crude glycoproteins were extracted with 0.15 M NaCl from the pooled endometrial scrapings of rabbit uteri after treatment with estrogen. The crude glycoproteins were fractionated with ammonium sulfate, followed by DEAE-cellulose column chromatography, treatment with CM-Sephadex C-25 and gel filtration on Sephadex G-200. Subsequently, purification of an acidic glycoprotein was carried out by gel filtration on Sephadex G-200 and then Sepharose 4B. The results of electrophoresis and enzymatic digestion, together with analytical data and the infrared spectrum indicated that the acidic glycoprotein was a sulfated glycoprotein.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D011817:Rabbits; D012794:Sialic Acids / Q000032:analysis; D013463:Sulfuric Acid Esters / Q000378:metabolism; D014599:Uterus / Q000378:metabolism*
D000596:Amino Acids; D004967:Estrogens; D006023:Glycoproteins; D006595:Hexosamines; D006601:Hexoses; D012794:Sialic Acids; D013463:Sulfuric Acid Esters