title: The influence of acridine dyes and caffeine on recovery from ultraviolet damage in Eudorina elegans.
abstract: Caffeine and the acridine dyes, acridine orange and acriflavine, were used to examine the repair potential in Eudorina elegans following ultraviolet irradiation. Acridines blocked photoreactivation primarily as a result of absorption of photoreactivating wavelengths, but acridines did not influence dark survival. Therefore, an acridine-sensitive excision-resynthesis-repair process is absent in Eudorina. Caffeine decreased both dark and light survival, the latter only after relatively high doses of ultraviolet light were used for inactivation. The caffeine-sensitive repair process appears to function most actively when the organisms are engaged in DNA synthesis, indicating that a postreplication-repair system exists in Eudorina. However, the data suggest that a repair system not associated with the DNA synthetic phases may also exist.
mesh_terms: D000166:Acridines / Q000494:pharmacology*; D000167:Acriflavine / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000187:drug effects*; D003624:Darkness; D056890:Eukaryota / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D008027:Light; D011830:Radiation Effects; D014466:Ultraviolet Rays
chemical_list: D000166:Acridines; D000167:Acriflavine; D002110:Caffeine; D004247:DNA