Histochemical localization of trehalase activity in dorsal flight muscle of the flesh fly Sarcophaga bullata with light and electron microscopy.
Trehalase activity in flight muscle of the flesh fly Sacrophaga bullata is detected histochemically at light- and electron-microscopic levels by using diaminobenzidine, glucose oxidase and peroxidase in the incubation medium. The association of trehalase activity with the inner mitochondrial membrane is confirmed. Biochemical assay shows that about 50% of the initial total trehalase activity is lost from the tissue during the histochemical processing and about 50% remains for histochemical detection.
D000818:Animals; D004175:Diptera / Q000201:enzymology*; D005426:Flight, Animal; D005949:Glucose Oxidase; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D008854:Microscopy, Electron; D009132:Muscles / Q000201:enzymology* / Q000648:ultrastructure; D014198:Trehalase / Q000032:analysis*
D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D014198:Trehalase