Changes in prostaglandin concentration in blood subjected to repetitive freezing and thawing.
Successive freezing and thawing of whole blood results in a consistently higher yield of various prostaglandin (PG) compounds. Evaluations were made with radioimmunological assay. The increase in PG concentrations seems to be more associated with cell fragmentation and not with the dissociation of albumin-PG complex. Our data suggest that there may be some dissociation of non-albumin-PG complexes. Artifactually high PG concentrations due to in vitro PG synthetase activity appears minimal at least with respect to indomethacin blocking of this enzyme. There are, in general, only slight differences in PG concentrations in samples with and without indomethacin.
D001793:Blood Preservation; D005260:Female; D005615:Freezing; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D011453:Prostaglandins / Q000097:blood*; D011485:Protein Binding; D013048:Specimen Handling; D013997:Time Factors
D011453:Prostaglandins; D007213:Indomethacin