title: Characterization of a naturally occurring diamine auxotroph of Veillonella alcalescens.
abstract: Veillonella alcalescens strain ATCC 17745 was shown to require putrescine or cadaverine for growth. None of the other compounds tried, including magnesium and spermidine, were able to substitute for the diamines. Studies with labeled diamines showed that spermidine was made from putrescine in this organism. A polyamine analogous to spermidine, but made from cadaverine, was not found. A combination of growth experiments and chemical assays suggested that protein synthesis was limited in diamine-starved cells. Protein synthesis occurred prior to nucleic acid synthesis when putrescine was added to starved cells.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002103:Cadaverine / Q000378:metabolism; D003959:Diamines / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D009154:Mutation; D011073:Polyamines / Q000096:biosynthesis; D011700:Putrescine / Q000378:metabolism; D012329:RNA, Bacterial / Q000096:biosynthesis; D013095:Spermidine / Q000096:biosynthesis / Q000378:metabolism; D013096:Spermine / Q000096:biosynthesis; D014678:Veillonella / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003959:Diamines; D011073:Polyamines; D012329:RNA, Bacterial; D013096:Spermine; D008274:Magnesium; D002103:Cadaverine; D013095:Spermidine; D011700:Putrescine