[Purification of creatine kinase from beef heart mitochondria].
53-fold purified creatine kinase is isolated from beef heart mitochondria by phosphate buffer extraction followed by chromatography on DEAE-cellulose and KM-cellulose and preparative electrophoresis in phosphate buffer density gradient. The purified enzyme was homogenous under electrophoresis in agarose gel and moved to cathode. The enzyme did not enter into separating gel under disc electrophoresis in conditions for the separation of neutral anc acid proteins, while under conditions for separating alkaline proteins it produced five fractions. The stability of creatine kinase under storage considerably decreased after the purification.
D000818:Animals; D002417:Cattle; D003402:Creatine Kinase / Q000302:isolation & purification* / Q000378:metabolism; D004355:Drug Stability; D008931:Mitochondria, Muscle / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D013997:Time Factors
D003402:Creatine Kinase
Ochistka kreatinkinazy iz mitokhondriĭ serdtsa byka