Identification of carcinogenic acetates of fluorenylhydroxamic acids by high-pressure liquid chromatography.
A method for the identification and quantitation of carcinogeniic O-acetates of fluorenylhydroxamic acids by high-pressure liquid chromatography is described. The adsorbent is Corasil II and a mixture of ethyl acetate-n-hexane (1:1) is used as the solvent. N-Acetoxy-2-fluorenylacetamide or N-acetoxy-3-fluorenylacetamide are separable as single peaks from N-acetoxy-4-fluorenylacetamide and from N-acetoxy-2-fluorenylbenzamide. The peak height traced by the recorder is linearly proportional to the amount of compound in the effluent. The method can be utilized for the detection of 0.1-1.0 mug of compound. The method has been used to identify the products of the decomposition of N-acetoxy-2-fluorenylacetamide in aqueous media.
D000099:Acetoxyacetylaminofluorene / Q000031:analogs & derivatives / Q000032:analysis*; D000327:Adsorption; D001549:Benzamides / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005449:Fluorenes / Q000032:analysis*; D007536:Isomerism; D008832:Microchemistry; D012997:Solvents
D001549:Benzamides; D005449:Fluorenes; D012997:Solvents; D000099:Acetoxyacetylaminofluorene