Radioimmunoassay of colchicine.
Antibodies to cholchicine were raised in rabbits after injection of N-desacetylthiocolchicine conjugated to bovine serum albumin. Antisera diluted 1:100 bound 50% of 1.6ng of [3H]colchicine. Cross-reaction of the antisera with colchicine derivatives and other drugs was measured and a radioimmunoassay of colchicine in biological fluids was proposed.
D000818:Animals; D000918:Antibody Specificity; D002850:Chromatography, Gel; D003078:Colchicine / Q000032:analysis* / Q000276:immunology; D006241:Haptens; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay
D006241:Haptens; D003078:Colchicine