[Intracellular distribution of creatine kinase isoenzymes in the brains and hearts of rats at different stages of postnatal development].
The total activity and range of the creatine kinase (CK) isozymes have been studied in the homogenate and subcellular fractions (nuclei, mitochondria, cytoplasm) of the rat brain and heart during postnatal ontogenesis. The total activity of CK in the brain and heart of newborn rats was found to be 4 and 2 times less, resp., than in those of adults. The age patterns were established in the activity of cytoplasmic (CK-1, CK-2 and CK-3) and mitochondrial (CK-4) isozymes. During the whole postnatal development the rat brain contains only one cytoplasmic isozyme, CK-1. In the heart of newborn rats, as compared with adults, the content of CK-1 and CK-2 is much higher and that of CK-3 lower. On the 12-15th day of life the range of the CK isozymes approaches that characteristic of adult animals. The activity of CK-4 was found in the brain on the 5-7th day of life and in the heart on 12-15th day. In the range of the CK isozymes in the adult brain the content of mitochondrial CK amounts to 19.3% and in the heart to 16.5%. The data obtained complement the literary ones suggesting the low level of energy-forming processes in the brain and heart cells at the early stages of the rat postnatal development.
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure; D002467:Cell Nucleus / Q000201:enzymology; D003402:Creatine Kinase / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology; D006321:Heart / Q000254:growth & development; D007527:Isoenzymes / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats
D007527:Isoenzymes; D003402:Creatine Kinase
Vnutrikletochnoe raspredelenie izofermentov kreatinkinazy v golovnom mozge i serdtse krys na razlichnykh stadiiakh postnatal'nogo razvitiia