Significance of the presence of the various subtypes for the preparation of HBAb.
The superficial Australia antigen is essentially bound to the small particles and possesses different antigenic specificities 'a', 'd', 'g', 'w', and 'r'. The population which we tested was characterized by the major frequency (more than 90%) of the 'ag' subtype with respect to the 'ad' subtype. The doses have been achieved using the solid phase RIA, modified according to Ling, in which the specificity of the antisera anti 'a', 'd' and 'g' was obtained by means of the absorption and dissociation of the antigen-antibody complexes. The investigations which we carried out to establish the avidity of the various anti-HBs anti-sera used for research into the Au antigen, have established that in general the avidity is maximal for the anti-ad and minimal or absent for the anti-ag. From this arises the necessity for the preparation of an anti-HBs anti-serum 'pool' consisting of healthy and hepatitic donors, with an optimal relationship between the subtypes 'ad' and 'ag'. The subsequent research carried out using the true subtypes in order to establish a relationship between the subtype and the presence of the anticore antibody on RIA revealed the lack of any correlation.
D000208:Acute Disease; D002908:Chronic Disease; D006509:Hepatitis B / Q000276:immunology*; D006511:Hepatitis B Antigens / Q000032:analysis*; D006801:Humans; D012703:Serotyping
D006511:Hepatitis B Antigens