title: Nucleotide sequence of 5-S RNA from Bacillus licheniformis.
abstract: The complete nucleotide sequence of 5-S RNA from Bacillus licheniformis was determined by analysis of complete and partial digests obtained with either T1 or pancreatic ribonuclease. The molecule was found to have a length of 116 nucleotides and may possess a minor sequence heterogeneity. There is a large degree of homology between the sequence of B. licheniformis 5-S RNA and those published for 5-S RNA from B. megatherium and B. stearothermophilus. The difference between the three 5-S RNA species are limited mainly to the two terminal and one internal sequence. B. licheniformis 5-S RNA contains the sequence U95-G-A-G-A-G100, which in B. subtilis has been implicated in the processing of precursor 5-S RNA. Possible models for the secondary structure of prokaryotic 5-S RNA are discussed on the basis of the results of limited digestion of B. licheniformis 5-S RNA by ribonuclease T1.
mesh_terms: D001407:Bacillus / Q000032:analysis*; D001483:Base Sequence; D001665:Binding Sites; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D012329:RNA, Bacterial / Q000032:analysis*; D012260:Ribonucleases; D013045:Species Specificity; D013104:Spheroplasts / Q000032:analysis
chemical_list: D009841:Oligonucleotides; D012329:RNA, Bacterial; D012260:Ribonucleases