Two factors affecting the heat stability of xanthine oxidase in extracts of mouse intestine.
The heat stability at 56 C of xanthine oxidase (Xox) from mouse intestine was found to be affected by two factors: (1) Xox which had been partially digested by trypsin was less heat stable than Xox which was protected from digestion by the presence of soybean trypsin inhibitor and phenylmethylsulfonyl fluoride; (2) Xox was less heat stable in glass tubes than in polystyrene tubes and siliconized glass tubes resembled polystyrene tubes in this respect.
D000818:Animals; D004355:Drug Stability; D005898:Glass; D006358:Hot Temperature; D007421:Intestine, Small / Q000201:enzymology*; D008297:Male; D051379:Mice; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011137:Polystyrenes; D014357:Trypsin / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000378:metabolism*
D011137:Polystyrenes; D014361:Trypsin Inhibitors; D010664:Phenylmethylsulfonyl Fluoride; D014969:Xanthine Oxidase; D014357:Trypsin