title: Electrophoretic, immunologic and kinetic characterization of erythrocyte pyruvate kinase in the Basenji dog with pyruvate kinase deficiency.
abstract: The electrophoretic mobility and the immunologic specificity of erythrocyte pyruvate kinase (PK) of the homozygous Basenji dog with PK deficiency were identical to those of normal M2-type PK isozyme seen in the white cell but not to those of the erythrocyte PK isozyme. Kinetic properties and stability were also consistent with the M2-type PK isozyme. Defective PK in the homozygous red cell was due to the absence of the erythrocyte PK isozyme and the compensatory presence of M2-type PK isozyme, as seen in the severe classical type PK deficiency in man.
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000201:enzymology*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology*; D006579:Heterozygote; D006720:Homozygote; D007106:Immune Sera; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D011770:Pyruvate Kinase / Q000172:deficiency* / Q000276:immunology / Q000302:isolation & purification; D013154:Spleen / Q000201:enzymology
chemical_list: D007106:Immune Sera; D011770:Pyruvate Kinase