[Characteristics of the hydrodynamic and biological properties of flagellar preparations isolated from submerged cultures of S. typhi].
A study was made of the hydrodynamic and biological properties of the flagella from the S. typhi 4446 cultures, isolated by the methods of differential centrifugation, in crude condition and following depolimerization and denaturing by the action of chemical and physical agents. Molecular parameters of the slow and rapid components of the flagella and subunits of the flagellin were compared. The greatest antigenic activity was possessed by the high molecular fraction of the flagella isolated in the 60% sucrose density gradient.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000942:Antigens, Bacterial / Q000032:analysis; D055592:Biophysical Phenomena; D001703:Biophysics; D003470:Culture Media; D005407:Flagella / Q000276:immunology*; D005408:Flagellin / Q000032:analysis; D007114:Immunization; D008970:Molecular Weight; D011817:Rabbits; D012485:Salmonella typhi / Q000166:cytology* / Q000276:immunology
D000942:Antigens, Bacterial; D003470:Culture Media; D005408:Flagellin
Kharakteristika gidrodinamicheskikh i biologicheskikh svoĭstv preparatov zhgutikov, vydelennykh iz glubinnykh kul'tur S. typhi