[Crystallin synthesis in the lens of newborn mice].
Lenses of newborn mice were incubated for different time in the Hanks solution containing 14C-amino acids mixture. Syntheses of gamma-crystallin and subunits of alpha-crystallin were shown to start at the first minute of the incybation. The incorporation rate of 14C-amino acids into gamma-crystallin was twice as high as that into alpha-crystallin within 5 minutes of the incubation. The assembly of alpha-crystallin tetramers took place after 5 minutes from the beginning of the incubation. Preincubation with actinomycin D for 3 and 6 hours resulted in the decrease of 14C-amino acids incorporation into gamma-crystallin only. These data suggest that the synthesis of gamma-crystallin takes place on both short-lived and long-lived mRNAs. Alpha-Crystallin subunits are supposed to synthesize only on long-lived mRNAs.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D003459:Crystallins / Q000096:biosynthesis*; D003609:Dactinomycin / Q000494:pharmacology; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
D000596:Amino Acids; D003459:Crystallins; D012333:RNA, Messenger; D003609:Dactinomycin
Izuchenie sinteza kristallinov v khrustalike novorozhdennykh mysheĭ