title: Studies on the brush border membrane of the mouse duodenum. I. Membrane isolation and analysis of protein components.
abstract: Brush border membranes have been isolated from villus epithelial cells of the adult Swiss mouse duodenum. Preparations of these membranes are not contaminated by other organelles as judged from electron-micrographs of sectioned pellets of brush borders. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins from brush borders solubilized in Tris-sodium dodecyl sulfate buffer reveals a reproducible Coomassie Brilliant Blue pattern of 17 bands. By comparing the brush border protein band positions with those of standard proteins run concurrently on sodium dodecyl sulfate-polyacrylamide gel slabs it is estimated that the 17 brush border proteins and subunits have molecular weights ranging from over 250,000 to around 16,000. Periodate-fuchsin sulfite staining shows that the five more slowly migrating, high molecular weight proteins are glycoproteins. The two proteins of smallest molecular size react positively with Oil Red O but have very small amounts of lipophilic amino acid residues, which indicates that the lipid extractable from the gels in these areas is a contaminant and is not bound to the proteins.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000032:analysis* / Q000648:ultrastructure; D004386:Duodenum / Q000032:analysis*; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis; D007413:Intestinal Mucosa / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D008857:Microscopy, Interference; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*
chemical_list: D008055:Lipids; D011506:Proteins