[The effect physical factors and proteolytic enzymes on actinomycete mycelium and spores].
The influence of enzymes (lyzozyme, trypsin, papain) and physical factors (hydrostatic pressure, mechanical sand grinding, extrusion in the frozen state, ultrasonic treatment) on the spores and mycelium of actinomycetes Actinomyces streptomycini B-6 and Thiobacillus vulgaris 2681 was studied. Actinomycete spores proved to be stable to trypsin and papain. Lyzozyme induced an almost complete disruption of the mycelium. The influence of hydrostatic pressure and lyzozyme on spores was the least effective. The best disruption of spores was produced by ultrasonic treatment and extrusion in the frozen state. The intensity of coloration of pigmented supernatants was the highest during ultrasonic disruption of spores.
D000191:Actinomycetaceae / Q000648:ultrastructure*; D005615:Freezing; D009113:Muramidase; D010206:Papain; D010447:Peptide Hydrolases; D011312:Pressure; D013172:Spores, Fungal / Q000648:ultrastructure; D014357:Trypsin; D014465:Ultrasonics
D009113:Muramidase; D010447:Peptide Hydrolases; D014357:Trypsin; D010206:Papain
Deĭstvie nekotorykh fizicheskikh faktorov i proteoliticheskikh fermentov na mitseliĭ i spory aktinomitsetov