Translation of honeybee promelittin messenger RNA. Formation of a larger product in a mammalian cell-free system.
Venom glands of honeybees synthesize the peptide melittin via the precursor promelittin. Total RNA preparations from venom glands served as template in a cell-free system prepared from mammalian cells. The heterologous system translated the insect mRNA with approximately the same efficiency as hemoglobin mRNA. A polypeptide was synthesized which, as shown by acrylamide gel electrophoresis in the presence of detergent, has a higher molecular weight than promelittin. Analysis of peptic fragments as well as Edman degradation have demonstrated that sequences characteristic of venom gland promelittin are present in this product formed in vitro. Furthermore, a bacterial protease which specifically splits after acidic residues liberates from the cell-free product a fragment which closely resembles melittin. Evidence is presented that most of the extra amino acids are located at the amino terminus of the product formed in vitro. The larger polypeptide detected in vitro may represent a precursor of promelittin.
D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees; D002474:Cell-Free System; D005260:Female; D008555:Melitten / Q000096:biosynthesis*; D051379:Mice; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D013698:Templates, Genetic; D014688:Venoms / Q000096:biosynthesis*
D011498:Protein Precursors; D012333:RNA, Messenger; D014688:Venoms; D008555:Melitten