Chromosomal proteins of rat brain: increased synthesis and affinity for DNA following a pulse of the carcinogen ethyinitrosourea in vivo.
A single pulse of ethlnitrosourea (EtNU), administered to 10-day-old BD IX-rats, specifically results in a high incidence of neuroectodermal tumors in the central and peripheral nervous system. At five days after an EtNU-pulse, analyses of protein-DNA interactions were performed using chromatin dissociation and re-association experiments, following incorporation of radioactive leucine into brain chromosomal proteins (CP) during short-term suspension culture. In comparison with 15-day-old control animals, the brain cells of EtNU-treated rats exhibited (i) an increased rate of CP synthesis, and (ii) an increased affinity of the newly-synthesized CP for brain DNA of both control and EtNU-treated animals.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005038:Ethylnitrosourea / Q000494:pharmacology*; D009607:Nitrosourea Compounds / Q000494:pharmacology*; D009698:Nucleoproteins / Q000096:biosynthesis* / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats
D002843:Chromatin; D009607:Nitrosourea Compounds; D009698:Nucleoproteins; D004247:DNA; D005038:Ethylnitrosourea