Analysis of macromolecule-macromolecule interactions by the moving boundary method.
A constituent velocity equation has been derived which permits analysis of macromolecule (P)--macromolecule (A) interactions in which a number of complexes, PA, PA2, PA3,---,PAn, are in rapid equilibrium. In previous treatments, it was assumed that the velocity of complex PAi (i = 1,2,3,---,n) increased linearly with i, an assumption limiting the validity of the resulting equation. In the present treatment, the velocity of PAi is expressed in the form of a polynomial function in i and a generalized equation has been formulated.
D004586:Electrophoresis; D008024:Ligands; D046911:Macromolecular Substances; D008433:Mathematics; D008722:Methods; D008968:Molecular Conformation; D008970:Molecular Weight; D011485:Protein Binding; D014461:Ultracentrifugation
D008024:Ligands; D046911:Macromolecular Substances