Influence of bile flow, theophylline and some organic anions on the biliary excretion of 3H-ouabain in rats.
The influence of some organic anions and theophylline on the biliary excretion of 3H-ouabain in bile-fistula rats (treated with 55 nmoles X kg(-1) 3H-ouabain i.p.) was investigated. Compared with controls (31.5 +/- 1.6 nmoles X kg(-1) per 3 hr) the excreted amounts of the glycoside within 3 hr were decreased to the following values: 22.3 +/- 0.9 nmoles X kg(-1) by taurocholic acid (0.7 nmoles X kg(-1) X hr(-1); 25.6 +/- 1.7 nmoles X kg(-1) by sulfobromophthalein (0.15 nmoles X kg(-1); 19.3 +/- 2.0 nmoles X kg(-1) by phenolphthalein (0.5 nmoles X kg(-1) and 20.9 +/- 2.1 nmoles X kg(-1) by probenecid (1.5 nmoles X kg(-1). Taurocholic acid and probenecid increased the bile flow by about 50 per cent compared to the controls as well as the biliary excretion of Na+, K+ and Cl-. Ethacrynic acid (0.2 nmoles X kg(-1) and the phospho-diesterase inhibitor theophylline (1.1 nmoles X kg(-1) had no influence on bile flow, electrolyte-and ouabain excretion. Therefore no relation between the biliary transport of ouabain and the bile flow could be observed. The interference of the hepatic transport of ouabain with organic anions is discussed in view of the current concept of a specific hepatic transport for steroid compounds.
D000818:Animals; D001646:Bile / Q000187:drug effects / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D010042:Ouabain / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology*; D013997:Time Factors
D002712:Chlorides; D010042:Ouabain; D012964:Sodium; D013806:Theophylline; D011188:Potassium