title: Fat metabolism in higher plants. Production of short- and medium-chain acyl-acyl carrier protein by spinach stroma preparations treated with cerulenin.
abstract: Incubation of stroma preparations from spinach chloroplasts with low concentrations of cerulenin (10 muM) resulted in severe inhibition of fatty acid synthesis but stimulated the release of medium-chain acids in very high proportions (60-70%). Preincubation of these preparations with cerulenin in the absence of substrate exerted no additional effect on subsequent fatty acid synthesis (as measured by incorporation of [14C]acetate into fatty acids) or the pattern of radioactive acids obtained. Acyl-protein, acyl-CoA, free fatty acids and lipids were resolved from each other and analysed for their distribution of 14C-labelled fatty acids. Acyl-protein derived from cerulenin-treated preparations was the only fraction which contained short- and medium-chain acids (C6--C12). The other fractions from both control and cerulenin-treated groups consisted exclusively of C16 and C18 acids. Acyl-protein was purified by gel filtration chromatography and was characterized as acyl-acyl carrier protein.
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002352:Carrier Proteins / Q000096:biosynthesis*; D002569:Cerulenin / Q000494:pharmacology*; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D003065:Coenzyme A / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D008055:Lipids / Q000096:biosynthesis; D010940:Plant Proteins / Q000096:biosynthesis; D010944:Plants / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014238:Trichloroacetic Acid / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D002352:Carrier Proteins; D005227:Fatty Acids; D008055:Lipids; D010940:Plant Proteins; D002569:Cerulenin; D014238:Trichloroacetic Acid; D003065:Coenzyme A