Isolation and properties of a fragment of human serum albumin demonstrating the absence of a methionine residue from some of the albumin molecules.
1. A fragment designated D was isolated from human serum albumin degraded by CNBr. Its properties show that it is made up of the B and C fragments isolated by McMenamy et al. (1971) (J. Biol. Chem., 246, 4744-4750). 2. Reduction of fragment D gives rise to two chains, one of which consists of the second subfragment of reduced fragment B linked to fragment C by an amino acid different from methionine. It thus demonstrates the existence of albumin molecules from which the second methionine residue located between fragments B and C is missing.
D000596:Amino Acids / Q000032:analysis; D000941:Antigens; D011232:Chemical Precipitation; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007120:Immunochemistry; D008715:Methionine / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis / Q000302:isolation & purification*; D014461:Ultracentrifugation
D000596:Amino Acids; D000941:Antigens; D012709:Serum Albumin; D008715:Methionine; D003488:Cyanogen Bromide