Differentiation of melanocytes in cultures of primary malignant melanoma indicated by 5-S-cysteinyldopa formation.
The dedifferentiation of cultured primary human malignant melanocytes was not accompanied by disappearance of 5-S-cysteinyldopa formation. The addition of conditioned medium from undifferentiated fibroblast-like cells brought about the reappearance of pigmented melanocytes and the increase of the metabolite in the cells and culture medium. The presence of 5-S-cysteinyldopa in cultured cells indicated the melanocytic origin of undifferentiated cells, and the increase of this metabolite was characteristic of differentiation.
D002454:Cell Differentiation; D002478:Cells, Cultured; D003545:Cysteine / Q000031:analogs & derivatives* / Q000378:metabolism; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D008544:Melanocytes / Q000378:metabolism* / Q000473:pathology; D008545:Melanoma / Q000378:metabolism*
D004295:Dihydroxyphenylalanine; D003545:Cysteine