Microfluorometric measurement at low temperature.
A cooling chamber for microfluorometric measurement is described, which allows to cool under a microscope a biological sample till 77 K and to measure it with an objective of a numerical aperture 0.6. From first experiments with BAO (5)-stained Tradescantia pollen it can be concluded, that experiments in this range of temperature open new aspects regarding the interpretation of microfluorometric phenomena.
D003080:Cold Temperature; D005453:Fluorescence; D005470:Fluorometry / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000379:methods*; D011058:Pollen / Q000032:analysis; D012034:Refrigeration / Q000295:instrumentation