Experimental production of aneuploidy in mouse oocytes.
The normal course of meiosis can be interrupted in cultured mouse oocytes by exposre to low temperature (23 degrees C). Re-transfer of the oocytes to a 37 degrees C environment will permit the meiotic process to resume and progress as usual to second metaphase. However, a significant number of oocytes treated in this manner prove to contain abnormal numbers of chromosomes.
D000782:Aneuploidy; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D008540:Meiosis; D051379:Mice; D009865:Oocytes / Q000648:ultrastructure; D010063:Ovum; D013696:Temperature