title: Liposome spin immunoassay: a new sensitive method for detecting lipid substances in aqueous media.
abstract: A new sensitive immunoassay procedure is described for quantitative detection of glycolipids and other lipids in aqueous media. As with other immunoassays specific antiserum is first reacted with the free lipid hapten. The amount of antibody activity remaining is measured by assaying the release, in the presence of complement, of spin label marker from liposomes containing the same lipid hapten. Using this method, 2.6 pmol of aqueous Forssman hapten was detected, and the sensitivity could be increased further.
mesh_terms: D000937:Antigen-Antibody Reactions; D005577:Forssman Antigen / Q000032:analysis; D006017:Glycolipids / Q000032:analysis* / Q000276:immunology; D007118:Immunoassay / Q000379:methods; D008081:Liposomes
chemical_list: D006017:Glycolipids; D008081:Liposomes; D005577:Forssman Antigen