[Reliable micromethod for determination of the protein content in tissue homogenates].
A micromodification for protein determination in tissue material using Amido black 10 B is described. Compared with the method of LOWRY et al. it requires a comparable time expenditure, but has three principal advantages: 1) it is 5-10fold more sensitive; 2) the calibration curve is linear over a virtually unlimited range; 3) it is feasible in the presence of a number of substances frequently used in protein analyses and making difficult or impossible measurement according to LOWRY et al.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002021:Buffers; D002540:Cerebral Cortex / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D007202:Indicators and Reagents; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008846:Micromanipulation; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D014793:Visual Cortex / Q000378:metabolism
D002021:Buffers; D007202:Indicators and Reagents; D011506:Proteins
Eine störungsfreie Mikromethode zur Bestimmung des Proteingehaltes in Gewebehomogenaten