title: Isolation and partial characterization of human eosinophil granules. Comparison to neutrophils.
abstract: Human blood eosinophils obtained from untreated patients with large numbers of circulating eosinophils were purified and lysed. An eosinophil contains 2.65 times as much peroxidase, 2.44 times as much beta-glucuronidase, approximately two times as much acid beta-glycerophosphatase, and 1.2 times as much protein as a neutrophil. Lysate filtration allowed isolation of eosinophil granules by isopycnic ultracentrifugation in sucrose. The granules had a mean density of rho 1.24 g/ml, and contained peroxidase, beta-glucuronidase, and acid beta-glycerophosphatase. They totally lacked muramidase and alkaline phosphatase. Electron micrography confirmed the isolation.
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000032:analysis; D002458:Cell Fractionation; D003594:Cytoplasmic Granules / Q000032:analysis*; D004804:Eosinophils / Q000032:analysis* / Q000201:enzymology / Q000648:ultrastructure; D005966:Glucuronidase / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009113:Muramidase / Q000032:analysis; D009504:Neutrophils / Q000032:analysis; D010544:Peroxidases / Q000032:analysis; D011506:Proteins / Q000032:analysis
chemical_list: D011506:Proteins; D010544:Peroxidases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D009113:Muramidase; D005966:Glucuronidase