Activation of lipase by a factor present in the gall-bladder epithelium.
The action of a bovine gall-bladder extract is studied on two lipolytic systems: the pancreas lipase and the plasma lipoproteinlipase. For pancreas lipase the lipolytic activity of pancreas homogenates of different species is evaluated in the presence of different quantities of gall-bladder extract. For plasma lipoproteinlipase, the enzyme is induced by injection of optimal doses of heparin associated with different quantities of gall-bladder extract and the activity is evaluated by measuring the clarifying power of the plasmas of the treated animals. The results confirm that the gall-bladder contains a factor activating the pancreas lipase and put in evidence some differences between lipase activities in different species. For plasma lipolytic activation, the action of the gall-bladder extract is particularly evident in the inductive phase of lipoproteinlipase, according to a typical process of saturation.
D000818:Animals; D002417:Cattle; D004285:Dogs; D005704:Gallbladder / Q000502:physiology*; D006168:Guinea Pigs; D006493:Heparin / Q000494:pharmacology; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000096:biosynthesis / Q000097:blood*; D010179:Pancreas / Q000201:enzymology*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013552:Swine; D014020:Tissue Extracts
D014020:Tissue Extracts; D006493:Heparin; D008049:Lipase; D008071:Lipoprotein Lipase