Effects of pyridoxine deficiency on the composition of plasma and liver fatty acids in rats fed low and high fat diets.
A study was made of the effect of pyridoxine deficiency on the fatty acids of liver and plasma in rats fed diets containing 1, 10, or 20% fat. Both ad libitum-fed and pair-fed control rats were used. In pyridoxine deficiency, arachidonic acid decreased with a concomitant increase in linoleic acid. Incubation of liver slices with [1-14C]acetate showed no significant alteration of fatty acid synthesis in pyridoxine-deficient rats fed 1 or 10% fat when compared with the ad libitum-fed control rats, but revealed a marked increase in cholesterogenesis compared with either control group. Incubations with [1-14C]linoleate resulted in no differences in the synthesis of arachidonate between the experimental and the ad libitum-fed control rats. Incubations with [5,6,8,9,11,12,14,15-3H]arachidonate showed decreased incorporation of this fatty acid in total liver lipids in pyridoxine-deficient rats compared with ad libitum fed control rats. We concluded that the diminution in arachidonate observed in liver lipids of pyridoxine-deficient rats does not result from a lower conversion of linoleate into arachidonate, but possibly from an increased degradation of this fatty acid.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage*; D005227:Fatty Acids / Q000378:metabolism*; D008041:Linoleic Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D014280:Triglycerides / Q000378:metabolism; D026681:Vitamin B 6 Deficiency / Q000378:metabolism*
D001095:Arachidonic Acids; D004041:Dietary Fats; D005227:Fatty Acids; D008041:Linoleic Acids; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol