[Preparation of labelled DNA from animal tissues].
Incorporation of 3H-thymidine into cells of bone marrow, spleen and intestinal mucosa was studied in rats with anemia, caused by administration of phenyl hydrazine-HCl. Optimal duration for administration of the labelled precursor was determined and the doses of 3H-thymidine required to produce 3H-DNA with the definite specific radioactivity were established. The isolation of the labelled preparations of DNA from rat spleen after incorporation of 2-2.5 muCi of 3H-thymidine is described; their physico-chemical properties were studied. The specific radioactivity of 3H-DNA was equal to 5-10(6)--10-10(6) disintegrations per 1 min per 1 mg.
D000740:Anemia / Q000139:chemically induced / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification*; D007413:Intestinal Mucosa / Q000378:metabolism*; D009928:Organ Specificity; D010659:Phenylhydrazines; D051381:Rats; D013154:Spleen / Q000378:metabolism*
D010659:Phenylhydrazines; D004247:DNA
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