title: On the fluorimetric determination of oestriol in pregnancy urines after thin layer chromatography.
abstract: An improved method is described for the determination of oestriol concentrations in urines of pregnant women. Oestriol is detected as a derivate of 1-dimethylaminonaphtalene-5-sulphonylchloride (DANSYLchloride). The method has the following characteristics: 1. The recovery of oestriol-16-glucuronide is 94%. 2. For the interpretation of the results obtained with this method mean values and 95% probability bounds of urinary oestriol excretion for each week of the last three months of pregnancy are given. (e.g. 49--125 mumol/24 h at 32 weeks of gestation, 108--238 mumol/24 h at 40 weeks). 3. The coefficient of variation of the method is 4% (from day to day). 4. The lowest detectable concentration is 3 mumol/l. 5. Influence of glucose can be eliminated. 6. Other urinary compounds have no influence on the determination.
mesh_terms: D001724:Birth Weight; D002855:Chromatography, Thin Layer / Q000379:methods; D003619:Dansyl Compounds; D004964:Estriol / Q000652:urine*; D005260:Female; D006029:Glycosuria; D006801:Humans; D007231:Infant, Newborn; D008832:Microchemistry; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D011336:Probability; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D003619:Dansyl Compounds; D004964:Estriol