In vitro test of heart muscle viability.
The purpose of the present study was to establish viability criteria for hearts perfused with a non-biological synthetic perfusate in a Langendorff type preparation. During hypothermic perfusion, the only discriminant between eight undamaged and four hypoxically damaged hearts was resistance to perfusion flow. At near normothermic conditions, the damaged hearts exhibited a decreased respiratory rate. It was also suggested that adrenalin stress of the isolated heart might be useful instead of orthotopic transplantation, as a final test of viability.
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology; D004987:Ethers / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D007773:Lactates / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D009924:Organ Culture Techniques; D010101:Oxygen Consumption; D010477:Perfusion; D011188:Potassium / Q000378:metabolism; D011312:Pressure; D011817:Rabbits; D012964:Sodium / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014022:Tissue Survival
D004987:Ethers; D007773:Lactates; D002245:Carbon Dioxide; D012964:Sodium; D003402:Creatine Kinase; D005947:Glucose; D011188:Potassium; D004837:Epinephrine