Characterization of IgG-containing DEAE fractions of canine serum.
A high-titered canine antilymphocyte serum was chromatographed on DE-52 cellulose and the fractions were assayed for cytotoxic activity. Although cytotoxicity was associated with the first 63% of the column effluent corresponding to fractions 1 through 7, significant activity (256) was demonstrable only in fractions 2, 3, 4, and 5. An immunoelectrophoretic study of fractions 1 through 5 showed only IgGa in fraction 1, IgGa and IgGb as electrophoretically separate arcs in fraction 2, and as electrophoretically inseparable arcs in fractions 3 through 5. Cytotoxicity is postulated to be associated with the IgGb subclass, since titers correspond with the distribution of IgGb in column fractions.
D000818:Animals; D000961:Antilymphocyte Serum / Q000032:analysis* / Q000302:isolation & purification; D002848:Chromatography, DEAE-Cellulose; D003601:Cytotoxicity Tests, Immunologic; D004285:Dogs / Q000276:immunology*; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis*
D000961:Antilymphocyte Serum; D007074:Immunoglobulin G