[Role of monovalent cations in reactions catalyzed by glycerol dehydrase from Aerobacter aerogenes].
Cobamide-dependent glyceroldehydrase (GDH) is shown to have an absolute requirement in monovalent cations: K+, NH4+, Tl+, Rb+ and Cs+. Dependencies of initial dehydratation rates of three substrates: glycerol, ethyleneglycol and 1,2-propandiol on the concentration of K+ are studied. Km values for K+, NH4+ and Tl+ are calculated to be 7-10-3, 4-10-3 and 1-10-3 M respectively. Effect of K+ on Km values for glycerol and coenzyme and on maximal reaction rate is investigated. It is shown that the apparent affinity of the substrate to the enzyme does not depend on monovalent cation; the apparent affinity of the coenzyme somewhat changes with the change of K+ concentration. Maximal reaction rate increases with the increase of K+ content. On the basis of kinetic data obtained possible mechanism of the activating effect of monovalent cations in reactions, catalyzed by GDH, is discussed.
D002414:Cations, Monovalent; D003038:Cobamides / Q000494:pharmacology; D004754:Enterobacter / Q000201:enzymology*; D004755:Enterobacteriaceae / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D006836:Hydro-Lyases / Q000378:metabolism*; D007700:Kinetics; D011188:Potassium / Q000494:pharmacology
D002414:Cations, Monovalent; D003038:Cobamides; D006836:Hydro-Lyases; D011188:Potassium