Inhibitory effect of prostaglandin E2 on the incorporation of 3H-thymidine into the DNA of thymus cells.
When rat thymic cells suspended in a simple salt-glucose-albumin medium were incubated with 10 mug/ml Prostaglandin E2 (PGE2) an inhibition of incorporation of 3H-thymidine into DNA was observed. PGE1 had a similar effect, while PGB2 and arachidonic acid proved to be ineffective. This inhibitory effect of PGE2 could be detected after a lag period of about 30 min but in the second hour of incubation the rate of incorporation of 3H-thymidine decreased to about 60 per cent of the control value. The extent of inhibition was found to be proportional to the log10 of the PGE2 concentration. Autoradiography showed that PGE2 had no influence on the number of DNA synthesizing cells. As an effect of PGE2 an early transient increase in the labeling of thymidine triphosphate was observed while the levels of labeled thymidine in the cells were not found to be significantly different in the presence of PGE2.
D000818:Animals; D004261:DNA Replication / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D013936:Thymidine / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism*
D011458:Prostaglandins E; D013942:Thymine Nucleotides; D013936:Thymidine