Cell damage and dye reduction in the quantitative nitroblue tetrazolium (NBT) test.
Nitroblue tetrazolium (NBT) is toxic to neutrophils; an effect which is greatly enhanced by endotoxin and latex particles. Cell damage, measured by the release of the cytoplasmic marker enzyme lactate dehydrogenase (LDH), was closely related to dye reduction. This suggests that, in this test, dye reduction occurs largely as a result of contact between intracellular reducing compounds and NBT following damage of the outer cell membrane. The expression of dye reduction as a function of LDH release should enhance the sensitivity of the quantitative NBT test by correcting for the observed intersubject variation in cell damage. The relationship between cell damage and dye reduction is a measure of the reducing capacity of the cell. This was normal in immature, bone marrow neutrophils, but diminished in neutrophils of patients with chronic granulomatous disease.
D001854:Bone Marrow Cells; D002462:Cell Membrane; D002469:Cell Separation; D004731:Endotoxins; D006105:Granulomatous Disease, Chronic / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D007840:Latex; D008863:Microspheres; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism*; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010587:Phagocytosis; D013778:Tetrazolium Salts / Q000378:metabolism*
D004731:Endotoxins; D007840:Latex; D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium; D007770:L-Lactate Dehydrogenase