[Comparison of the activities of free and carrier fixed horseradish peroxidase].
Commerical horseradish-peroxidase was covalently bound to BrCN-activated sepharose. The activity parameters Vmax and Km were determined by the leukomalachite green reaction. Compared with the soluble enzyme, the immobilized POD has a relative residual activity of 22%. Dimethylsulfoxide and formamide were found to diminish the enzymatic activity in a concentration-dependent manner. The activity of the free enzyme in aqueous formamide solution (10%) is reduced by 43%, that of the insolubilized enzyme by 68%. Dimethylsulfoxide (15%) does not alter the LMG conversion rate of free POD, whilst a rate loss by 60% was observed for the immobilized enzyme.
D001665:Binding Sites; D003488:Cyanogen Bromide; D004121:Dimethyl Sulfoxide; D005559:Formamides; D006735:Horseradish Peroxidase / Q000494:pharmacology*; D010544:Peroxidases / Q000494:pharmacology*; D012685:Sepharose; D012995:Solubility
D005559:Formamides; D012685:Sepharose; D006735:Horseradish Peroxidase; D010544:Peroxidases; D003488:Cyanogen Bromide; D004121:Dimethyl Sulfoxide
Aktivitätsvergleich von freier und trägerfixierter Meerrettich-Peroxidase