Influence of temperature on the detectibility and chromosomal distribution of specific DNA sequences by in situ hybridisation.
Hybridising certain AT-rich satellite complementary RNAs (cRNAs) to their homologous chromosomal DNA sequences at different temperatures of incubation results in a different dispersion of autoradiographic label throughout the karyotypes. The temperature at which most label, or cRNA-DNA hybrid formation, exists corresponds to the optimal rate temperature for the hybridisation of these same satellite cRNA-DNA hybrids as determined by RNA excess filter hybridisation. It is likely that the in situ hybridisation results can therefore be explained by the fact that there is a similar temperature-dependence on the rate of hybrid formation for both in situ and RNA excess hybridisation. This should have important implications for the designing of in situ hybridisation experiments in general.
D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D004276:DNA, Satellite / Q000032:analysis*; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D012313:RNA; D013696:Temperature
D004276:DNA, Satellite; D012313:RNA; D004247:DNA