[Ribosomal RNA synthesis in haploid and diploid loach embryos].
rRNA synthesis was compared in the loach haploid (In) and diploid (2n) embryos. The relative intensity of synthesis was evaluated by 14C-uridine incorporation in 27S and 18S rRNA isolated from ribosomes taking into account label incorporation into total acid-soluble fraction and phosphrylated uridine derivatives. Label incorporation into rRNA, in reference with DNA content in 1n and 2n embryos, suggests that the level of rRNA synthesis per DNA unit in haploids is twice that in diploids whereas, in reference per cell, the same amount of ribosomes is synthesized both in haploids and diploids. The data obtained show that the amount of rRNAs synthesized in the loach embryogenesis does not depend on ploidy.
D000818:Animals; D004171:Diploidy; D005399:Fishes / Q000196:embryology* / Q000378:metabolism; D006238:Haploidy; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D014529:Uridine / Q000378:metabolism
D012335:RNA, Ribosomal; D014529:Uridine
Sintez ribosomnykh RNK v gaploidnykh i diploidnykh embrionakh v'iuna