[DNA and protein synthesis in cultured lens epithelial cells. I. Test system].
An in vitro test system for measuring DNA and protein synthesis in cultivated lens epithelium cells was developed. The method is suited also for other monolayer cultures; it has the following advantages: a) Cultivation of cells, incubation with radionuclides, preparation of the samples and measurement of radioactivity are carried out in the same vessel (scintillation vial); b) The use of 3H-thymidine and 14C-phenylalanine allows simultaneous measurement of DNA and protein synthesis; c) Only small amounts of cells (10(4) to 10(5) cells) are required to measure DNA and protein synthesis. The test system is highly sensitive to synthetic effectors (cytosone arabinoside, actinomycin D, puromycin), and is thus appropriate for the detection of inhibitors of DNA and protein synthesis and for testing the toxicity of drugs.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003459:Crystallins / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism*; D000431:Ethanol; D007908:Lens, Crystalline / Q000378:metabolism; D000432:Methanol; D008722:Methods; D010649:Phenylalanine / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014357:Trypsin
D003459:Crystallins; D000431:Ethanol; D010649:Phenylalanine; D004247:DNA; D014357:Trypsin; D013936:Thymidine; D000432:Methanol
DNS- und Protein-Synthese in kultivierten Linsenepithelzellen. I. Testsystem