[Study of toxin from burned skin].
By means of gel filtration, ion exchange chromatography and polyacrylamide gel disc electrophoresis the toxin from burned rat skin was characterized as a protein with molecular weight 300,000 containing 3-5 subunits. Dissociation of the initial complex was caused by ion exchange chromatography, electrophoresis in fine pore gels and under storage. The dissociation was shown to become irreversible in presence of detergents.
D000818:Animals; D001665:Binding Sites; D002056:Burns / Q000378:metabolism*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011485:Protein Binding; D014176:Protein Biosynthesis; D051381:Rats; D012867:Skin / Q000378:metabolism*; D014118:Toxins, Biological / Q000096:biosynthesis*
D046911:Macromolecular Substances; D014118:Toxins, Biological
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