Oxygen uptake by suspension of mouse brain cells.
A cell suspension was prepared by sieving mouse brain cortices in an isotonic  solution of purified polyvinylpyrrolidone. A large increase on the O2-uptake by the suspension could be obtained with the preparation procedure described. The respiratory rate of suspension, incubated in saline medium, containing 6.2 mM K+, 10 mM pyruvate, 5 mM fumarate and 0.9 mM 5'-AMP, was equal to 68% of the respiratory rate of slices. High K+ concentration (65 mM) stimulated O2-consumption of suspension by 64% (73% in slices).
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D005650:Fumarates / Q000494:pharmacology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D010101:Oxygen Consumption / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology; D011205:Povidone / Q000494:pharmacology; D011773:Pyruvates / Q000494:pharmacology
D005650:Fumarates; D011773:Pyruvates; D000249:Adenosine Monophosphate; D011205:Povidone; D011188:Potassium