The development of antisera to prostaglandins B2 and F2alpha and their analysis using solid-phase and double antibody radioimmunoassay methods.
1 A method for the production of highly substituted prostaglandin-bovine serum albumin conjugates has been developed. 2 Antisera to prostaglandins B2 and F2alpha were raised in rabbits immunized with prostaglandin-bovine serum albumin conjugates. 3 The antisera were assessed for specificity and sensitivity by the double antibody radioimmunoassay method and after they were covalently linked to powdered cellulose to form a 'solid-phase' system. 4 Solid phase radioimmunoassays were developed using conventional shaking and in the presence of sucrose which obviates the need for continuous mixing of the incubates.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D000918:Antibody Specificity; D008297:Male; D011453:Prostaglandins / Q000276:immunology*; D011460:Prostaglandins F / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D012710:Serum Albumin, Bovine; D013997:Time Factors
D000906:Antibodies; D011453:Prostaglandins; D011460:Prostaglandins F; D012710:Serum Albumin, Bovine