Alkali lability of bacteriophage phi W-14 DNA.
The molecular weight of bacteriophage phi W-14 DNA, determined by velocity sedimentation in neutral sucrose gradients, was 92 +/- 6 X 10(6). The DNA showed marked fragmentation in alkaline sucrose gradients. This fragmentation was not a consequence of preexisting single-strand interruptions in the DNA, since thermal denaturation of DNA yielded intact single strands. The alpha-putrescinylthymine groups in phi W-14 DNA appeared to be labile; some, or parts of some, of these groups were cleaved from the DNA in alkali.
D001435:Bacteriophages / Q000032:analysis* / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000032:analysis*; D006358:Hot Temperature; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D011549:Pseudomonas; D012260:Ribonucleases / Q000378:metabolism; D012972:Sodium Hydroxide / Q000494:pharmacology
D004279:DNA, Viral; D012972:Sodium Hydroxide; D012260:Ribonucleases