[Effects of Methotrexat on the chromosomes of a human fetus in vivo (author's transl)].
A pregnant woman in the 17th week of gestation with Hodgkin's disease was treated with 50 mg Methotrexate i.v. on the 3 days preceeding therapeutic abortion. Cytogenetic studies on blood, brain, skin and lung of the fetus were performed. Slight structural aberrations such as gaps, chromatid breaks and accentric fragments were found in an average of 8,5% of the counted metaphases. The following drastic structural chromosome aberrations were found in 24,8% of all observed metaphases: 1) mitoses with stretched chromosomes, comparable with special segments (9,9%). 2) clumping of chromosomes in varying degrees (5,4%). 3) combinations of 1) and 2) (0,4%). 4) nuclear fragments (7,4%). 5) pulverized chromosomes (1,7%). Endoreduplications were found in 2%. The modal number was 46 with a rate of 50,1%, hypodiploids 46,3%, hyperdiploids and polyploids 3,6%. Karyotype: 46, XX.
D000032:Abortion, Therapeutic; D001773:Blood Cells; D001921:Brain / Q000166:cytology; D002842:Chromatids / Q000648:ultrastructure; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D003582:Cytogenetics; D005260:Female; D005333:Fetus / Q000187:drug effects; D006689:Hodgkin Disease / Q000188:drug therapy; D006801:Humans; D008168:Lung / Q000166:cytology; D008727:Methotrexate / Q000627:therapeutic use; D008938:Mitosis / Q000187:drug effects; D011123:Polyploidy; D011247:Pregnancy; D011248:Pregnancy Complications; D012867:Skin / Q000166:cytology
D008727:Methotrexate
Wirkung von Methotrexat auf die Chromosomen eines menschlichen Foetus in vivo