[Fluorescence analysis study of the structural lability of biomembranes and their components. II. Higher fatty acids].
Binding constants, quantum yield and temperature relationship of the fluorescence of ionic (1-anilinonaphtalene-8-sulphonate-ANS) and neutral (N-phenyl-1-naphtalamine-PNA) probes have been studied on micellae and ufasomes of natural fat acids. The following regularities have been obtained: 1. The Anion dye (ANS) sharply decreases the intensity of fluorescence on fat acid structures while alkilating the medium, it is not practically bound with them at pH-7; 2. The neutral dye (PNA) shiws no fluorescence on micellae of the limited fat acids and is comparatively well bound with urasomes. The binding constant quantum yield of PNA fluorescence significantly decreases during the transition from the ufasomes of oleic acid to those of polyunsaturated fat acids. 3. The temperature relationship of PNA fluorescence intensity sorbed on oleic acid ufasomes shows twists in the region of 16--20degreesC and 46--52degreesC. The first transition is connected with the melting of potassium oleate.
D015057:1-Naphthylamine / Q000031:analogs & derivatives; D000817:Anilino Naphthalenesulfonates; D005231:Fatty Acids, Unsaturated; D005470:Fluorometry; D008823:Micelles
D000817:Anilino Naphthalenesulfonates; D005231:Fatty Acids, Unsaturated; D008823:Micelles; D015057:1-Naphthylamine
Issledovanie strukturnoĭ labil'nosti biomembran i ikh komponentov metodom fluorestsentnogo analiza. II. Vysshie zhirnye kisloty