[The influence of pyridoxal 5'-phosphate on the temperature relationships of aspartate aminotransferase isoenzymes].
The relationship between temperature and the behaviour of aspartate aminotransferase was investigated in the presence of pyridoxal 5'-phosphate. The addition in vitro of pyridoxal 5'-phosphate caused an increase in the activity and altered the thermal behaviour of aspartate aminotransferase. In choosing the temperature for the determination of enzymic activity, the concentration of the coenzyme must therefore also be considered.
D001219:Aspartate Aminotransferases / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D013696:Temperature; D013816:Thermodynamics
D007527:Isoenzymes; D011732:Pyridoxal Phosphate; D001219:Aspartate Aminotransferases
Der Einfluss von Pyridoxal-5'-phosphat auf das Temperaturverhalten der Aspartataminotransferase-Isoenzyme