A column chromatography fractionation of the hydroxyproline-containing urinary peptides with continuous automatic detection.
Dialyzable and non-dialyzable urinary hydroxyproline-containing peptides are chromatographed respectively on QAE-Sephadex and on phosphocellulose. They are detected and quantitated by continuous hydrolysis in 3.3 N NaOH followed by oxidation by chloramine T and colorimetry with p-dimethylamino-benzaldehyde. The patterns of dialyzable urinary hypropeptides do not show significant qualitative differences between normal subjects and patients suffering from Paget's bone disease or cancer metastases of bone. The patterns of non-dialyzable urinary hypropeptides, show more variability in the case of normal subjects and differ more largely in the case of Paget's disease of bone.
D001322:Autoanalysis / Q000379:methods; D001859:Bone Neoplasms / Q000652:urine; D005591:Chemical Fractionation; D002845:Chromatography; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006801:Humans; D006909:Hydroxyproline / Q000652:urine*; D007202:Indicators and Reagents; D009362:Neoplasm Metastasis; D010001:Osteitis Deformans / Q000652:urine; D010455:Peptides / Q000652:urine*
D007202:Indicators and Reagents; D010455:Peptides; D006909:Hydroxyproline