[Polymorphism and glycoprotein character of Ricinus communis lectins purified by affinity chromatography].
Two lectin fractions (S20W = 6,8 and 4,9 S) were purified from Ricinus communis seeds. The purification was carried out in four steps : ammonium sulfate fractionation, affinity chromatography on Sepharose 4 B, gel filtration on Sephadex G 150 and chromatography on CM celluloes. The purified lectins were glycoproteins whose chemical composition was determined. Amino terminal analysis of the two fractions revealed glycine and serine. Polyacrylamide gel electrophoresis of the higher molecular weight fraction allowed the separation of several components with different affinity for PAS staining.
D000017:ABO Blood-Group System; D000117:Acetylglucosamine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D002846:Chromatography, Affinity; D004912:Erythrocytes; D006023:Glycoproteins / Q000032:analysis*; D006384:Hemagglutination; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D009005:Monosaccharides / Q000032:analysis; D037121:Plant Lectins; D010947:Plants, Toxic; D012278:Ricinus / Q000032:analysis*
D000017:ABO Blood-Group System; D000596:Amino Acids; D006023:Glycoproteins; D037102:Lectins; D009005:Monosaccharides; D037121:Plant Lectins; D000117:Acetylglucosamine
Polymorphisme et caractère glycoprotétique des lectines de Ricinus communis purifiées par chromatographie d'affinité