Plasma hemoglobin determination in beagle dogs. An adaptation of the method of Crosby and Furth.
The plasma hemoglobin concentration of beagle dogs was measured following an improved bleeding technic which minimized trauma of the red cells. The benzidine reaction (benzidine dihydrochloride) was used to measure the hemoglobin present. Plasma hemoglobin values of the first 2 ml of blood collected were statistically significantly higher (P less than 0.01) than values of the next 2-3 ml of blood. Plasma hemoglobin values of normal beagles were 0.5-2.5 mg/dl in minimally hemolyzed samples. Recovery rates of up to 92% of hemoglobin added to plasma were possible with this method.
D000818:Animals; D004285:Dogs / Q000097:blood*; D005260:Female; D006452:Hemoglobinometry / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis*; D008297:Male
D006454:Hemoglobins