Quantification of physiological changes in gut endocrine cells: immunocytochemical and radioimmunoassays of secretin cell function.
A model experiment involving the response of secretin cells to acid perfusion of the duodenum has shown that, with suitable sampling methods, quantitative immunocytochemistry using Image Analysis provides results which closely match those obtained by the application of radioimmunoassay to tissue extracts.
D000818:Animals; D004386:Duodenum; D005768:Gastrointestinal Hormones / Q000032:analysis / Q000378:metabolism*; D006651:Histocytochemistry; D006851:Hydrochloric Acid; D007120:Immunochemistry; D007413:Intestinal Mucosa / Q000032:analysis / Q000378:metabolism*; D011863:Radioimmunoassay; D012633:Secretin / Q000032:analysis / Q000378:metabolism*; D013552:Swine
D005768:Gastrointestinal Hormones; D012633:Secretin; D006851:Hydrochloric Acid