title: Studies on the effect of bile salt and colipase on enzymatic lipolysis. Improved method for the determination of pancreatic lipase and colipase.
abstract: The rate of hydrolysis of long chain triglycerides by pure bovine pancreatic lipase has been determined in the presence of variable amounts of bile salts and colipase. Cofactor-free lipase is strongly inhibited by sodium taurodesoxycholate and by mixed bovine bile salts at concentrations higher than the critical micellar concentration. Bile salt inhibited lipase is reactivated by the addition of bovine colipase. Gel filtration of pancreatic juice from several species (Cow, dog, pig) on Sephadex G 100 allows the separation of lipase from colipase. It is found that the enzyme catalyzed hydrolysis of long chain triglycerides by pancreatic lipase from one species is activated by the addition of colipase from other species. Studies on the activation of pancreatic lipase by colipase in the presence of bile salts allowed the re-evaluation of optimal conditions for the determination of lipase and the development of a procedure to assay colipase.
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002417:Cattle; D003089:Colipases / Q000378:metabolism* / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D008049:Lipase / Q000378:metabolism*; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology*; D010189:Pancreatic Juice / Q000187:drug effects / Q000201:enzymology*; D011506:Proteins / Q000378:metabolism*; D013552:Swine; D014280:Triglycerides / Q000378:metabolism
chemical_list: D001647:Bile Acids and Salts; D003089:Colipases; D011506:Proteins; D014280:Triglycerides; D003840:Deoxycholic Acid; D008049:Lipase