[The interaction of pyridoxamine-5'-thiophosphate with aspartate apo-transaminase].
Interaction of 5'-bromopyridoxamine with thiophosphate yeld 5'-phosphate thio ester of pyridoxamine (I). Apotransaminase and I reacted in equimolar amounts; a stable complex with 10--15 per cent of activity formed exhibiting a positive CD at 335 nm. Incubation of the complex with alpha-ketoglutarate led to formation of corresponding aldimine form and to partial splitting of the S--P bond. The same splitting take place during incubation of the I--apo-enzyme complex with substrate mixture.
D001051:Apoenzymes / Q000378:metabolism; D001059:Apoproteins; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001665:Binding Sites; D002942:Circular Dichroism; D009946:Organothiophosphorus Compounds; D011485:Protein Binding; D011487:Protein Conformation; D011733:Pyridoxamine / Q000031:analogs & derivatives*
D001051:Apoenzymes; D001059:Apoproteins; D009946:Organothiophosphorus Compounds; D011733:Pyridoxamine; D001219:Aspartate Aminotransferases