Preliminary purification and dosages of the erythropoietic factor from ox-spleen.
The administration of ox-spleen homogenate, whether in rats made polycythemic by blood transfusion or in mice polycythemic by hypoxia, elicited a significant stimulatory effect on the rate of erythropoiesis. This activity remained unchanged even if the spleen, before being homogenized, was exhaustively washed with isotonic buffer pH 7.4 up to the total blood elimination.
D000818:Animals; D001772:Blood Cell Count; D002417:Cattle; D004912:Erythrocytes / Q000378:metabolism; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D006400:Hematocrit; D007501:Iron / Q000378:metabolism; D051379:Mice; D011086:Polycythemia / Q000378:metabolism; D051381:Rats; D012156:Reticulocytes; D013154:Spleen; D014020:Tissue Extracts / Q000494:pharmacology*
D014020:Tissue Extracts; D004921:Erythropoietin; D007501:Iron