Effect of glutamic acid on the fatty acid and lipid composition of Choanephora cucurbitarum.
The fatty acid composition of the total, neutral, sterol, free fatty acid, and polar-lipid fractions in the mycelium of Choanephora curcurbitarum was determined. The major fatty acids in all lipid fractions were palmitic, oleic, linoleic, and gamma-linolenic acid. Different lipid fractions did not show any particular preference for any individual fatty acid; however, the degree of unsaturation was different in different lipid fractions. Free fatty acid and polar lipid fractions contained a higher proportion of gamma-linolenic acid than did triglyceride and sterol fractions. Addition of glutamic acid to the malt-yeast extract and medium resulted in the biosynthesis of a number of long-chain fatty acids beyond the gamma-linolenic acid. These fatty acids, e.g., C22:1, C24:0, and C26:0, were never observed to be present in the fungus when grown on a malt-yeast extract medium without glutamic acid. Furthermore, thin-layer chromatographic analysis showed a larger and denser spot of diphosphatidyl glycerol from the mycelium grown on glutamic acid medium than from the control mycelium. The possible significance of this finding is discussed.
D003470:Culture Media; D005227:Fatty Acids / Q000032:analysis*; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005658:Fungi / Q000032:analysis*; D005971:Glutamates / Q000378:metabolism*; D008055:Lipids / Q000032:analysis*; D009090:Mucorales / Q000032:analysis* / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D013261:Sterols / Q000032:analysis; D014280:Triglycerides / Q000032:analysis
D003470:Culture Media; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D005971:Glutamates; D008055:Lipids; D010743:Phospholipids; D013261:Sterols; D014280:Triglycerides