[Determination of optimal conditions for physical and chemical inactivation of nuclei for obtaining haploid and anuclear loach (Misgurnus fossilis L.) embryos].
The doses of X-irradiation sufficient for obtained truly haploid and anuclear embryos of the loach were determined. Mitomycin C in concentrations arresting the development at the late blastula stage (100-500 mcg/ml) was tested for the purposes of chemical enucleation of embryos. The data obtained allowed to recommend the following doses of X-irradiation to obtained the 100% inactivation of one or both the paternal genomes and haploid and anuclear embryos of the loach: 40 kr for eggs and 60 kr for testes. Mitomycin in the concentrations tested did not cause the 100% enucleation of embryos.
D000818:Animals; D002467:Cell Nucleus; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000528:radiation effects; D005399:Fishes / Q000196:embryology*; D006238:Haploidy; D008937:Mitomycins / Q000494:pharmacology*; D011830:Radiation Effects; D014965:X-Rays
D008937:Mitomycins
Opredelenie optimal'nykh usloviĭ fizicheskoĭ i khimicheskoĭ inaktivatsii iader dlia polucheniia gaploidnykh i bez'iadernykh zarodysheĭ v'iuna Misgurnus fossilis (L.)