A comparative study of the effects of lucanthone (miracil D) and actinomycin D on the Chinese hamster cells grown in cultures.
Chinese hamster cells of an established clone line grown in monolayers were incubated for up to two hours with either lucanthone (0.3-30 mug/ml) or actinomycin D (0.06-0.10 MUG/ML) AND SUbjected to radioautographic investigations with 3H-uridine during the period of treatment. At concentration of 9 mug/ml lucanthone selectively inhibited the synthesis of nucleolar (ribosomal) RNA while the extranucleolar RNA synthesis proceeded at a high level. Similar results were obtained with 0.08 mug/ml actinomycin D. Protein synthesis and mitotic activity were also affected by lucanthone but the drug did not markedly interfere with DNA synthesis. Lucanthone appeared to be much less effective in cell killing than  actinomycin D and its inhibitory effects on the nucleolar RNA synthesis and other cellular processes proved readily reversible. The results allow to conclude that lucanthone may be useful as a tool for studying RNA synthesis in animal cells.
D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008154:Lucanthone / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D014316:Tritium; D003609:Dactinomycin; D012313:RNA; D004247:DNA; D008154:Lucanthone; D014529:Uridine