Gas metabolism in ovine rumen cultures on a nitrogen-deficient medium.
Inocula of rumen fluid from sheep were grown on a medium of low nitrogen content under an oxygen-free atmosphere containing N2. The cultures produced methane and carbon dioxide. Hydrogen gas was utilized and the partial pressure of N2 decreased by 24% in 48 h. Assuming this N2 to have been reduced to metabolically available nitrogenous compounds, these account for about 0.2% of the sheep's daily need.
D000693:Anaerobiosis; D000818:Animals; D001128:Argon / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D003470:Culture Media; D005260:Female; D005740:Gases / Q000378:metabolism*; D005750:Gastric Juice / Q000382:microbiology*; D006859:Hydrogen / Q000378:metabolism; D008697:Methane / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010313:Partial Pressure; D012756:Sheep
D003470:Culture Media; D005740:Gases; D002245:Carbon Dioxide; D001128:Argon; D006859:Hydrogen; D009584:Nitrogen; D008697:Methane