title: [Photic induction of polar growth in enucleated fragments of Acetabularia mediterranea].
abstract: The process of induction of polarity in enucleated fragments (EF) of Acetabularia mediterranea has been studied by the method of local illumination. A device is described which allows to illuminate one part of EF at 2, 500 lx and another at 5 lx. The local illumination of a part of EF during 10-24 hrs was shown to polarize with a high probability the subsequent growth and formation of caps at the subsequent uniform illumination. Using the method of successive illumination and darkening of apical and basal portions of EF, it was shown that the initial stages of induction were partially reversible and the complete determination of polarity took place after the initiation of the cap anlage. After the short-term induction by light (2500 lx), the darkening of the growth zone inhibits completely the process of cap initiation, but already at 100 lx the EF previously induced at 2 500 lx are initiated and grow. While using the local illumination of grafts of EF from two species, A. crenulata and A. mediterranea, with their subsequent fragmentation and the analysis of cap morphology, it was shown that the induction of polar growth did not provide the directed transport of the factors controlling morphogenesis to the future growth zone.
mesh_terms: D000076:Acetabularia / Q000254:growth & development*; D000460:Chlorophyta / Q000254:growth & development*; D009024:Morphogenesis; D010775:Photic Stimulation
vernacular_title: Induktsiia svetom poliarnogo rosta u énukleirovannykh fragmentov Acetabularia mediterranea