[A new bifunctional reagent for the intramolecular crosslinking of insulin (author's transl)].
Reaction of bis-[2-(succinimidooxycarbonyloxy)ethyl]sulfone [SO2(Eoc-ONSu)2] with insulin in 1N NaHCO3/dimethylformamide forms NalphaA1,NepsilonA1,NepsilonB29-2,2'-sulfonylbis(ethoxycarbonyl)insulin [SO2(Eoc)2-insulin] in 20 - 35% yield. The product can be purified by partition chromatography. After cleavage of the disulfide bridges, reoxidation in very dilute solution reconstitutes about 60% of the original insulin activity. Cleavage of the crosslinking moiety can be achieved with 0.5N NaOH at 0 degrees C in only a few seconds, rendering a biologically fully active insulin.
D001665:Binding Sites; D004220:Disulfides / Q000032:analysis; D007202:Indicators and Reagents; D007328:Insulin; D046911:Macromolecular Substances; D010084:Oxidation-Reduction; D011485:Protein Binding; D011487:Protein Conformation; D013388:Succinimides; D013450:Sulfones
D004220:Disulfides; D007202:Indicators and Reagents; D007328:Insulin; D046911:Macromolecular Substances; D013388:Succinimides; D013450:Sulfones
Ein neues bifunktionelles Reagens zur intramolekularen Vernetzung von Insulin