[Peculiarities of RNA synthesis in sections of rat liver tissues and cells of chicken embryos under conditions of protein synthesis inhibition].
Kinetics of radioactive predecessors incorporation into RNA and proteins of the rat liver and chicken embryos cells was studied as affected by different doses of cycloheximide. In both cases a definite dependence is established for changes in the specific radioactivity of nuclei and cytoplasm RNA on the antibiotic concentration: the doses of cycloheximide which cause an insignificant inhibition of protein biosynthesis do not evoke an increase in the intensity of RNA biosynthesis. Intermediate doses of cyclohemide in certain periods of incubation stimulate incorporation of 2-14C-orotic acid into RNA both in experiments with the tissue sections and with cell culture. A dependence is supposed to be between the intracellular bond of the changes in RNA biosynthesis on a degree of protein biosynthesis inhibition.
D000818:Animals; D002642:Chick Embryo; D003513:Cycloheximide / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D009963:Orotic Acid / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D013045:Species Specificity; D014158:Transcription, Genetic / Q000187:drug effects*
D009963:Orotic Acid; D012313:RNA; D003513:Cycloheximide
Osoblyvosti syntezu RNK v zrizakh tkanyny pechinky shchuriv i klitynakh embrioniv kureĭ v umovakh pryhnichennia syntezu bilka