title: Measurement of umbilical blood flow in fetal lambs in utero.
abstract: We continuously measured umbilical blood flow in fetal lambs in utero by placing an electromagnetic flow transducer around the common umbilical artery. Umbilical arteries originate from a short common segment as the terminal branches of the descending aorta. This segment was isolated by a retroperitoneal surgical approach and encircled with a specially constructed electromagnetic flow transducer. Catheters were also placed in fetal vessles to monitor pressure and derive flow values by the radionuclide-labeled microsphere technique. The fetus and ewe were allowed to recover for two days before studies were performed. Average umbilical blood flow obtained in 11 animals with the transducer was 199 ml/kg per min. In seven animals flow measurements obtained with the transducer were compared with those derived from microsphere injections. Paired measurements varied by an average of only 5.3%. This technique makes possible the accurate and instantaneous measurement of umbilical blood flow in fetal lambs in utero over a prolonged period.
mesh_terms: D000818:Animals; D005260:Female; D005333:Fetus / Q000502:physiology*; D008722:Methods; D008863:Microspheres; D011247:Pregnancy; D012039:Regional Blood Flow; D012756:Sheep; D014159:Transducers; D014469:Umbilical Arteries / Q000502:physiology*