[Effects of colchicine on the content of ribonucleic acids and total proteins of cultured nerve cells. Preliminary study].
Nervous cells from chick embryo lumbo-sacral spinal cord have been cultivated in vitro and treated with colchicine. The effects of this alkaloid on the RNA and the total protein contents of nervous cells have been studied by quantitative cytochemical methods. The RNA content has been measured by cytophotometry after DNase digestion and gallocyanine-chromalun staining; the cellular protein content has been measured after Naphtol Yellow S staining. In colchicine treated cultures, the main part of the nervous cell population consists of spheroidal and piriform neuroblasts; some cells are neurocytes with short processes. During the 48 first hours in vitro, the RNA content is approximatively identical in treated cells as in controls. The cellular total protein content is much lower in the treated cells than in the controls.
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D003078:Colchicine / Q000494:pharmacology*; D009474:Neurons / Q000032:analysis / Q000187:drug effects*; D011506:Proteins / Q000032:analysis*; D012313:RNA / Q000032:analysis*; D013116:Spinal Cord / Q000166:cytology
D011506:Proteins; D012313:RNA; D003078:Colchicine
Effets de la colchicine sur la teneur en acides ribonucleiques et en proteines totales de cellules nerveuses cultivees in vitro