Four hour-test for the identification of Enterobacteriaceae.
A set of 12 rapid biochemical tests--lysinedecarboxylase, ornithinedecarboxylase, beta-galactosidase, urease, hydrogensulphide, indole, acetoin, deoxyribonuclease, esculin, mannitol, raffinose and sorbitol--were selected from an original set of 13 tests and were found to give 98% accurate reactions within 4 hrs of incubation for the identification of bacteria belonging to Enterobacteriaceae. This set permits identification on the genus and/or species level for Escherichia, Shigella, Citrobacter, Salmonella, Klebsiella, Enterobacter, Serratia and Proteus.
D000093:Acetoin / Q000096:biosynthesis; D001120:Arginine / Q000032:analysis; D003851:Deoxyribonucleases / Q000096:biosynthesis; D004755:Enterobacteriaceae / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism; D004929:Esculin / Q000378:metabolism; D005696:Galactosidases / Q000032:analysis; D006862:Hydrogen Sulfide / Q000378:metabolism; D007211:Indoles / Q000032:analysis; D008239:Lysine / Q000032:analysis; D008353:Mannitol / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000032:analysis; D011887:Raffinose / Q000378:metabolism; D013012:Sorbitol / Q000378:metabolism; D013997:Time Factors; D014510:Urease / Q000032:analysis
D007211:Indoles; D004929:Esculin; D008353:Mannitol; D013012:Sorbitol; D001120:Arginine; D000093:Acetoin; D003851:Deoxyribonucleases; D005696:Galactosidases; D014510:Urease; D009955:Ornithine Decarboxylase; D008239:Lysine; D011887:Raffinose; D006862:Hydrogen Sulfide