[The effect of mRNA on the stability of the ribosome--aminoacyl-tRNA complex].
The effect of some olygo- and polynucleotides on the dissociation rate of the 14C-aminoacyl-tRNA - ribosome complex was investigated. Polyuridylic and polycytidylic acids were shown to accelerate significantly dissociation of the complex of lysyl- and phenylalanyl-tRNA with native ribosomes, but not to affect the complexes of these aminoacyl-tRNA's with 50S subunits. It is proposed that the template polynucleotides decrease the affinity of ribosomes to tRNA by association with the mRNA-binding site on 30S subunits.
D001665:Binding Sites; D009841:Oligonucleotides / Q000494:pharmacology; D011066:Poly C / Q000494:pharmacology; D011072:Poly U / Q000494:pharmacology; D011119:Polynucleotides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000494:pharmacology*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D013698:Templates, Genetic
D009841:Oligonucleotides; D011119:Polynucleotides; D012333:RNA, Messenger; D011072:Poly U; D011066:Poly C; D012343:RNA, Transfer
Vliianie IRnk Na Stabil'noct' Komppleksa Ribosoma-- Aminoatsil-TRnk