[Effect of acetylcholine on phosphorylase from skeletal muscle].
Acetylcholine (1-10(-5) g/ml) was shown to increase the content of active form of phosphorylase A in homogenate of cat skeletal muscle. Atropine (1-10(-5) g/ml) diminished acetylcholine-induced increase in the phosphorylase activity from 22% to 5.28%. Activating effect of acetylcholine was observed also in superatant (105000 g), although it was 2-fold decreased. No acetylcholine-induced phosphorylase activation was observed in the presence of Mg2+. Addition of Ca2+ (2.2-10(-14) M) did not affect considerably the activation process. It is concluded that the mechanism of acetylcholine-induced phosphorylase activation is different from the effect of adrenaline on phosphorylase.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002415:Cats; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology*; D006005:Phosphorylases / Q000378:metabolism*
D001285:Atropine; D006005:Phosphorylases; D008274:Magnesium; D000109:Acetylcholine; D002118:Calcium; D004837:Epinephrine
Deistzie Atsetilkholina Na Fosforilazi Skeletnoi Myschtsy