Effect of estradiol on the early incorporation of (3H) thymidine into uterine DNA on immature mouse.
The incorporation of [3H]thymidine into uterine DNA was markedly depressed within 10 to 30 minutes after intraperitoneal administration of 17beta-estradiol to immature mouse. Maximum inhibition occurred about 6 hours after the hormone was administered. Uterine DNA content and the amount of [3H]thymidine incorporated into the acid-soluble fraction was not affected during the period of hormone-induced inhibition. Moreover, the in vitro incorporation of [3H]thymidine by isolated estradiol-treated mouse uterus was blocked. In contrast to the uterus, 17beta-estradiol did not influence the incorporation of thymidine into mouse liver DNA. Evidence is presented to show that the incorporation of thymidine into uterine DNA was blocked initially by 17beta-estradiol.
D000818:Animals; D002369:Castration; D004247:DNA / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000378:metabolism; D051379:Mice; D051381:Rats; D013936:Thymidine / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
D004958:Estradiol; D004247:DNA; D013936:Thymidine