title: Effect of detergents and sucrose derivatives on the modified protein determination of Lowry.
abstract: The effect on the protein determination method of Lowry, as modified by Hartreee, of widely used detergents (sodium deoxycholate, sodium laurylsulphate, Triton X-100 and Triton WR-1339) and of sugar derivatives often used for preparing concentration gradients (sucrose, dextran, Ficoll and Metrizamide) was examined. Each of the compounds studied gave rise to a coloured product in the test; however, the correlation between concentration and optical density was usually nonlinear. The majority of these substances - especially Ficoll, Metrizamide and dextran - interfere with the procedure even at low concentrations, thus their elimination from the sample before protein determination is inevitable.
mesh_terms: D002499:Centrifugation, Density Gradient; D003840:Deoxycholic Acid; D003902:Detergents; D003911:Dextrans; D005362:Ficoll; D008793:Metrizamide; D011092:Polyethylene Glycols; D011506:Proteins / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods; D013395:Sucrose
chemical_list: D003902:Detergents; D003911:Dextrans; D011506:Proteins; D003840:Deoxycholic Acid; D005362:Ficoll; D011092:Polyethylene Glycols; D013395:Sucrose; D008793:Metrizamide