Incorporation of Arginine-3H into chromatin of mouse eggs shortly after sperm penetration.
Mouse oocytes were fertilized in vitro in a complete cultivation medium enriched by L-arginine-5(3)H monohydrochloride. The oocytes were isolated four hours after insemination. The incorporation of this precursor, as detected by means of autoradiography, was significantly higher in swollen sperm heads and female chromosomes at anaphase/telophase of the second meiotic division than the incorporation into ooplasm.
D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001345:Autoradiography; D002843:Chromatin / Q000096:biosynthesis*; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D005306:Fertilization; D008297:Male; D008540:Meiosis; D051379:Mice; D008810:Mice, Inbred C57BL; D014176:Protein Biosynthesis; D013077:Sperm Head / Q000378:metabolism; D013997:Time Factors; D015053:Zygote / Q000378:metabolism*
D002843:Chromatin; D001120:Arginine