[Use of colour reactions to identify lipid classes, including tocopherols, by thin-layer chromatography].
Colour reactions of different classes and individual lipids of plant origin with organic and inorganic compounds (used as reagents) were studied to identify them in thin-layer silica gel. Some of the reagents (p-dimethyl phenylene diamine, chromotropic acid in H2SO4, Ti(SO4)2 in H2SO4) helped to detect main lipid classes and to identify selectively individual compounds (alpha- and gamma-tocopherols). Hydrochloric p-nitrophenyl diazonium, p-dimethyl aminobenzaldehyde are of interest mainly as group reagents to lipid classes with common properties. The reaction sensitivity is measured in micrograms.
D002855:Chromatography, Thin Layer / Q000379:methods; D007202:Indicators and Reagents; D008055:Lipids / Q000032:analysis*; D008832:Microchemistry; D010944:Plants / Q000032:analysis; D014810:Vitamin E / Q000032:analysis*
D007202:Indicators and Reagents; D008055:Lipids; D014810:Vitamin E
Ispol'zovanie nekotorykh tsvetnykh reaktsiĭ dlia identifikatsii otdel'nykh klassov lipidov, vkliuchaia tokoferoly, pri TSKh