Total synthesis of a structural gene for the human peptide hormone angiotensin II.
Seven oligonucleotide chains containing between 6 and 11 nucleotide units were synthesized. The segments were phosphorylated by T4 polynucleotide 5'-hydroxyl-kinase and joined by T4 polynucleotide synthetase (ATP) to give the double-stranded DNA consisting of 33 base pairs. The DNA sequence was deduced from the known peptide sequence according to the genetic code.
D000804:Angiotensin II / Q000096:biosynthesis*; D001483:Base Sequence; D003090:Coliphages / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis*; D005796:Genes; D005815:Genetic Code; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism; D011118:Polynucleotide Ligases / Q000378:metabolism; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D000804:Angiotensin II; D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D011118:Polynucleotide Ligases