Electrical properties of the cellular transepithelial pathway in Necturus gallbladder. I. Circuit analysis and steady-state effects of mucosal solution ionic substitutions.
Microelectrode techniques were employed to measure the electrical resistance of the cell membranes and the shunt pathway, and the equivalent electromotive forces (EMF's) at both cell borders in Necturus gallbladder epithelium. The cell is, on the average, 57 mV negative to the mucosal solution and 59 mV negative to the serosal solution. The transepithelial potential (Vms) ranges from 0.5 to 5 mV, serosal solution positive. Assuming that the shunt EMF (Vs) is zero with standard Ringer's bathing oth sides of the tissue, both cell membrane EMF's are oriented with the negative pole toward the cell interior and are 39.9 +/- 3.6 mV (apical, Va), and 69.4 +/- 1.8 mV (basal-lateral, Vb)...
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005704:Gallbladder / Q000502:physiology*; D008433:Mathematics; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D011188:Potassium / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D014562:Caudata
D012965:Sodium Chloride; D011188:Potassium