title: On the role of divalent cations in the reaction mechanism of malic enzyme.
abstract: The kinetic order of addition of Mg2+ and L-malate to malic enzyme has been determined. Mg2+ is the first to bind, and probably acts as a link between the substrate and the active site.
mesh_terms: D002413:Cations, Divalent; D003035:Cobalt / Q000494:pharmacology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology; D011549:Pseudomonas / Q000201:enzymology*
chemical_list: D002413:Cations, Divalent; D003035:Cobalt; D008345:Manganese; D008291:Malate Dehydrogenase; D008274:Magnesium