An improved method of assessing topical corticosteroid activity.
Topical application of ointment bases causes varying degrees of epidermal thickening in guinea-pigs. This is reproducible and can be accurately measured. Suppression of these changes was produced by addition of beta-methasone 17-valerate, fluocinolone acetonide, fluocinonide and hydrocortisone acetate. The inhibitory effect of these steroids was related to the type of corticosteriod, its concentration and the ointment base. The effect was still evident at extremely high dilutions of the steroids and could be measured at concentrations as low as 5 X 10(-5) % of fluocinonide in FAPG. This technique has the merits of being reproducible, sensitive and accurate. It should find a place among existing methods in assaying the efficacy of topical corticosteroids and in aiding in the selection of bases most suitable for formulation of these preparations.
D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001624:Betamethasone Valerate / Q000494:pharmacology; D001699:Biometry; D005446:Fluocinolone Acetonide / Q000494:pharmacology; D005447:Fluocinonide / Q000494:pharmacology; D006168:Guinea Pigs; D006854:Hydrocortisone / Q000494:pharmacology; D008722:Methods; D009823:Ointment Bases; D010592:Pharmaceutic Aids; D011409:Propylene Glycols; D012867:Skin / Q000187:drug effects*
D000893:Anti-Inflammatory Agents; D009823:Ointment Bases; D010592:Pharmaceutic Aids; D011409:Propylene Glycols; D005446:Fluocinolone Acetonide; D005447:Fluocinonide; D001624:Betamethasone Valerate; D006854:Hydrocortisone