title: Soluble melanoprotein of tumor origin.
abstract: The procedure for isolating soluble melanoprotein from human malignant melanoma was described. Electron microscopy confirmed the absence of any contaminating organized particles. The melanoprotein was brown hygroscopic substance with 8.8% of melanin, easily soluble in water. The solution revealed general absorption with a shoulder at 280 nm. Chemical composition was typical for a melanoprotein complex with characteristic high sulphur content (2.1%) and abundance in zinc (210 mug Zn/g dry sample). Sedimentation and electrophoretic properties indicated relative homogeneity. Similar substance could also be isolated from Harding-Passey mouse melanoma. Such soluble melanoproteins have been needed for their broad exploitation in tumor immunology.
mesh_terms: D005260:Female; D006801:Humans; D008545:Melanoma / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis / Q000302:isolation & purification*; D012878:Skin Neoplasms / Q000032:analysis*; D012995:Solubility
chemical_list: D009363:Neoplasm Proteins