[High-voltage electrophoresis and its application in combination with other methods for protein structure studies].
The method of high-voltage paper electrophoresis may be applied not only for peptide separation, but also in modifications and combinations with other methods, so, by means of aminoethylation and maleylation it is possible to broaden or narrow the range of trypsin action. This, in its turn, makes it possible to isolate preparatively lysin- and arginine-containing peptides, oxidation with performic acid enables the thyol-containing fragments to be isolated and application of carboxypeptidase A-C-terminal peptide of protein. When studying the primary structure of proteins the method has already found its widest application but with an increase in the number of methods of protein specific modification its potentiabilities will be even wider.
D002268:Carboxypeptidases / Q000032:analysis; D004590:Electrophoresis, Paper / Q000379:methods; D010446:Peptide Fragments / Q000032:analysis; D011506:Proteins / Q000032:analysis*
D010446:Peptide Fragments; D011506:Proteins; D002268:Carboxypeptidases
Vysokovol'tnyĭ elektroforez i ego ispol'zovanie v sochetanii s drugimi metodami pri issledovanii struktury belkov