Regulation of in vitro mRNA transcription by a fraction of chromosomal proteins.
A fractionation scheme was developed which permits the isolation of chromosomal non-histone protein fraction associated with DNA in chromatin. This fraction which represents less than 10% of the total protein content of reticulocyte chromatin was found to be essential for the in vitro transcription of globin mRNA by chromatin preparations reconstituted from DNA and isolated chromosomal protein components.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002645:Chickens; D002843:Chromatin / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism*; D004270:DNA, Circular / Q000302:isolation & purification; D003852:Deoxyribonucleoproteins / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D009693:Nucleic Acid Hybridization; D009698:Nucleoproteins / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic
D002843:Chromatin; D004270:DNA, Circular; D003852:Deoxyribonucleoproteins; D009698:Nucleoproteins; D012333:RNA, Messenger