Role of myoglobin in the oxygen supply to red skeletal muscle.
The contribution of nyoglobin to the oxygen uptake of red skeletal muscle was estimated from the difference in oxygen uptake with and without functional myoglobin. The oxygen uptake of bundles (25 mm long, 0.5 mm mean diameter) of muscle fibers teased from pigeon breast muscle was measured in families of steady states of oxygen pressure from 0 to 250 mm Hg. The oxygen-binding function of myoglobin, in situ in muscle fiber bundles, was abolished by treatment with nitrite of hydroxylamine, which convert oxymyoglobin in situ to high spin ferric myoglobin, or with phenylhydrazine, which converts oxymyoglobin to denatured products, or with 2-hydroxyethylhydrazine, which appears to remove myoglobin from the muslce. The oxygen uptake was again measured. At higher oxygen pressure, where oxygen availability does not limit the respiration of the fiber bundles, oxygen uptake is not affected by any of the four reagents, which is evidence that mitochondrial oxygen uptake is not impaired. At lower oxygen pressure, where oxygen uptake is one-half maximal, the steady state oxygen consumption is roughly halved by abolishing functional myoglobin. Under the steady state conditions studied, the storage function of myoglobin, being static, vanishes and the transport function stands revealed. We conclude from these experiments that myoglobin may transport a significant fraction of the oxygen consumed by muscle mitochondria.
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D010856:Columbidae; D003486:Cyanides / Q000494:pharmacology; D006834:Hydrazines / Q000494:pharmacology; D006898:Hydroxylamines / Q000494:pharmacology; D007700:Kinetics; D008931:Mitochondria, Muscle / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D009211:Myoglobin / Q000378:metabolism*; D009573:Nitrites / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D010313:Partial Pressure; D010659:Phenylhydrazines / Q000494:pharmacology; D013053:Spectrophotometry
D003486:Cyanides; D006834:Hydrazines; D006898:Hydroxylamines; D009211:Myoglobin; D009573:Nitrites; D010659:Phenylhydrazines; D000968:Antimycin A