Mannitol transport in Streptococcus mutans.
A hexitol-inducible, phosphoenolpyruvate-dependent phosphotransferase system was demonstrated in Streptococcus mutans. Cell-free extracts obtained from mannitol-grown cells from a representative strain of each of the five S. mutans serotypes (AHT, BHT, C-67-1, 6715, and LM7) were capable of converting mannitol to mannitol-1-phosphate by a reaction which required phosphoenolpyruvate and Mg2+. Mannitol and sorbitol phosphotransferase activities were found in cell-free extracts prepared from cells grown on the respective substrate, but neither hexitol phosphotransferase activity was present in extracts obtained from cells grown on other substrates examined. A heat-stable, low-molecular-weight component was partially purified from glucose-grown cells and found to stimulate the mannitol phosphotransferase system. Divalent cations Mn2+ and Ca2+ partially replaced Mg2+, while Zn2+ was found to be highly inhibitory.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001693:Biological Transport, Active; D002118:Calcium / Q000494:pharmacology; D002474:Cell-Free System; D005947:Glucose / Q000378:metabolism; D006600:Hexosephosphates / Q000096:biosynthesis; D008274:Magnesium / Q000494:pharmacology; D008353:Mannitol / Q000378:metabolism*; D008354:Mannitol Dehydrogenases / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D013012:Sorbitol; D013045:Species Specificity; D013291:Streptococcus / Q000378:metabolism*; D013295:Streptococcus mutans / Q000201:enzymology / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D006600:Hexosephosphates; D008353:Mannitol; D013012:Sorbitol; D010728:Phosphoenolpyruvate; D000429:Alcohol Oxidoreductases; D008354:Mannitol Dehydrogenases; D010770:Phosphotransferases; D008274:Magnesium; D005947:Glucose; D015032:Zinc; D002118:Calcium