Purification and characterization of guinea-pig intestinal brush borders.
The purification of guinea-pig intestinal brush borders by a rapid sucrose-gradient-centrifugation step is reported. A 29-fold increase in the maltase/DNA quotient indicates considerable purification of the brush borders from nuclei. The biological activity of the brush borders was well preserved, as demonstrated by a high recovery of human gastric-juice-mediated uptake of 57Co-labelled vitamin B-12; homogeneity and purity were confirmed by scanning electron microscopy. Both the morphological appearance and biological activity were unchanged after prolonged storage in glycerol.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000032:analysis; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000032:analysis / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D013347:Subcellular Fractions / Q000378:metabolism
D004247:DNA