title: Nickel deficiency and nickel-rhodium interaction in chicks.
abstract: Nickel deficiency was produced in chicks under near optimal growth conditions. This judgment is based on the finding that chicks fed the experimental diet supplemented with nickel had a very satisfactory growth rate, over 600 g in 4 weeks. To induce nickel deficiency, chicks were raised in plastic cages located inside plastic isolators and were fed diets (containing 2-15 ng of nickel/g) based on dried skim milk, acid-washed ground corn, EDTA-extracted soy protein, and corn oil. In 2 experiments, controls were fed 3 mug of nickel/g as NiCl2-6H2O. In experiment 3, instead of 1 control group 25, 50, 250, and 2,500 ng/g of supplemental dietary nickel as NiCl2-6H2O were each given to separate groups of chicks. Nickel deprivation resulted in: ultrastructural changes in the liver with the most obvious abnormality in the organization of the rough endoplasmic reticulum; altered gross appearance, reduced oxidative ability, and decreased lipid phosphorus in the liver; altered shank skin pigmentation that was associated with a decrease in yellow lipochrome pigments; and lower hematocrits. Deficiency also tended to increase the thickness of the legs and size of the hock; decrease the length:width ratios of the tibias and femurs; and decrease the plasma cholesterol. None of the signs of deficiency were seen in chicks fed diets containing at least 52 ng of nickel/g. In one experiment, a group of birds was fed 50 mug of rhodium/g of diet as (ClRh(NH3)5)SO4 to ascertain whether rhodium is a metabolic antagonist of nickel. Supplemental rhodium increased the hematocrits and liver oxidative ability of both nickel-deficient and -supplemented chicks, and increased total liver lipids, liver lipid phosphorus, and liver cholesterol in the nickel-deficient chicks alone. Rhodium did not increase the signs of nickel deficiency.
mesh_terms: D000818:Animals; D001835:Body Weight; D001846:Bone Development; D002645:Chickens / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D003677:Deficiency Diseases / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D004864:Equipment and Supplies; D006400:Hematocrit; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D009532:Nickel / Q000172:deficiency / Q000378:metabolism; D010101:Oxygen Consumption; D010860:Pigments, Biological / Q000378:metabolism; D010969:Plastics; D012238:Rhodium / Q000378:metabolism*; D012867:Skin / Q000378:metabolism
chemical_list: D010860:Pigments, Biological; D010969:Plastics; D009532:Nickel; D002784:Cholesterol; D012238:Rhodium