[Lateral diffusion of the protein components of the respiratory assembly of Microsoccus lysodeikticus].
Preparations with a selectively decreased (by 85-90%) content of NADH dehydrogenase were isolated by means of heating treatment of M. lysodeikticus isolated membranes. The degree of the reduction of the NADH dehydrogenase nearest neighbour in the respiration chain of cytochrome b556 in heated membranes is similar to that in intact membranes. It is concluded that cytochrome b556 and (or) NADH dehydrogenase are capable to lateral migration in the membrane of M. lysodeikticus, resulting in the inter-chain electrone transport. A coefficient of their lateral diffusion is calculated (D equals 8-10(-10)-2-10(-9) CM2SEC-1 At 30 degrees C) on the basis of kinetics of cytochrome reduction by NADH dehydrogenase. The electron transport, due to a diffusion of respiration carriers from one assambly to another, proceeds 100 times as slow as the electrone transport in the respiratory chain. The data obtained allow to consider the aggregation of respiration enzymes as a dynamic formation.
D001692:Biological Transport; D002462:Cell Membrane / Q000201:enzymology; D003580:Cytochromes / Q000378:metabolism*; D004058:Diffusion; D004579:Electron Transport; D007700:Kinetics; D046911:Macromolecular Substances; D008837:Micrococcus / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010101:Oxygen Consumption; D011485:Protein Binding
D003580:Cytochromes; D046911:Macromolecular Substances; D009247:NADH, NADPH Oxidoreductases
Lateral'naia diffuziia belkovykh komponentov dykhatel'nogo ansambiia Micrococcus lysodeikticus