Potentiation by caffeine of ultraviolet-light damage in cultured human cells.
Five cultured human cell lines (T-1 kidney, Chang liver, H.Ep. No. 2, HeLa-S3 and HeLa-O) were irradiated with ultraviolet light and immediately exposed to 1.0 and 3.0 mM caffeine for 44 h thereafter. This caffeine treatment reduced the surviving fraction (assayed by colony formation) of the irradiated population, but did not significantly reduce the colony-forming ability of unirradiated control cells. These findings suggest that many cultured human cell lines exhibit post-UV potentiation of potentially lethal damage by caffeine.
D002110:Caffeine / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured / Q000528:radiation effects*; D004247:DNA / Q000378:metabolism; D006801:Humans; D011831:Radiation Genetics; D014466:Ultraviolet Rays
D002110:Caffeine; D004247:DNA