Effects of environmental lighting, superior cervical ganglionectomy and adrenergic drugs on microtubule protein levels of the rat hypothalamus.
Bilateral superior cervical ganglionectomy or exposure of rats to light for 10 days resulted in comparable increases in microtubule protein content in the hypothalamus. Administration of norepinephrine, L-dopa or isoproterenol decreased hypothalamic tubulin levels to a greater extent in ganglionectomized rats. A prior injection of phentolamine prevented norepinphrine's effects in intact rats and decreased but did not abolish the effect of the catecholamine in ganglionectomized animals. Phentolamine impaired L-dopa activity in intact and denervated rats as did propranolol; beta-adrenergic blockage impaired the effect of norepinephrine in denervated rats and abolished that of isoproterenol. Phentolamine plus propranolol prevented norepinephrine's effects whereas it only impaired L-dopa activity on hypothalamic tubulin content. The prior administration of actinomycin D blocked the effects of norepinephrine, L-dopa or isoproterenol. Pinealectomy abolished the isoproterenol-induced decrease in microtubule protein content and impaired that following L-dopa. These data suggest that tubulin levels of the rat hypothalamus are controlled by adrenergic transmitter via alpha- and beta-receptors, the latter involving the pineal gland.
D000818:Animals; D001337:Autonomic Agents / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology; D005725:Ganglia, Autonomic / Q000378:metabolism / Q000601:surgery; D007031:Hypothalamus / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D007980:Levodopa / Q000494:pharmacology; D008027:Light; D008297:Male; D008870:Microtubules / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009434:Neural Pathways; D009638:Norepinephrine / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats
D001337:Autonomic Agents; D009419:Nerve Tissue Proteins; D003609:Dactinomycin; D007980:Levodopa; D011433:Propranolol; D007545:Isoproterenol; D009638:Norepinephrine; D010646:Phentolamine