Isolation of isoflavones inhibiting DOPA decarboxylase from fungi and streptomyces.
By screening of culture filtrates of fungi and streptomyces for activity in inhibit dopa decarboxylase the following isoflavone compounds were obtained: psi-tectorigenen (I), genistein (II), orobol (IV), 8-hydroxygenistein (V) and a new compound (III). III was elucidated to be 3', 4', 5, 7-tetrahydroxy-8methoxy isoflavone. Among these isoflavones, IV and III showed the strongest activity in inhibiting dopa decarboxylase. All these isoflavones also inhibited histidine decarboxylase and catechol-O-methyltrasnferase. Activities of these compounds to inhibit tyrosine hydroxylase and dopamine beta-hydroxylase were examined. Orobol which showed no or only slight inhibition of tyrosine hydroxylase and dopamine beta-hydroxylase exhibited a significant hypotensive effect on spontaneously hypertensive rats.
D000818:Animals; D000959:Antihypertensive Agents; D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D001230:Aspergillus / Q000032:analysis*; D001234:Aspergillus niger / Q000032:analysis*; D005419:Flavonoids / Q000302:isolation & purification*; D006640:Histidine Decarboxylase / Q000037:antagonists & inhibitors; D007529:Isoflavones / Q000302:isolation & purification* / Q000494:pharmacology; D008750:Methyldopa / Q000494:pharmacology; D051379:Mice; D051381:Rats; D013302:Streptomyces / Q000032:analysis*
D000959:Antihypertensive Agents; D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D005419:Flavonoids; D007529:Isoflavones; D008750:Methyldopa; D006640:Histidine Decarboxylase