Production of antibodies to amanitins as the basis for their radioimmunoassay.
The production of antibodies against amanitins is described. By means of these antibodies, a radioimmunoassay was developed which allows detection of as little as 0.5 ng of amanitins in 1 ml of serum. By this method, the clearance of alpha-amanitin from the blood of poisoned mice was measured.
D000546:Amanitins / Q000097:blood / Q000276:immunology*; D000818:Animals; D005620:Freund's Adjuvant; D008297:Male; D051379:Mice; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats / Q000276:immunology; D012709:Serum Albumin
D000546:Amanitins; D012709:Serum Albumin; D005620:Freund's Adjuvant