title: Heterogeneous distribution of DNA alkylation products in rat liver chromatin after in vivo administration of N,N-di[14C]methylnitrosamine.
abstract: Poly-l-lysine (PL) binds to about 50% of chromatin DNA, rendering it resistant to degradation by DNAase I. Separation of the unbound DNA as acid-soluble nucleotides allows the fractionation of chromatin DNA into two zones. After in vivo administration of N,N-di[14C]methylnitrosamine, the amount of alkylation in DNA was found to be lower in the polylysine-binding regions. Some possible reasons for this heterogeneous distribution are discussed.
mesh_terms: D000478:Alkylation; D000818:Animals; D001665:Binding Sites; D002843:Chromatin / Q000187:drug effects*; D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects*; D008297:Male; D009602:Nitrosamines / Q000494:pharmacology*; D011107:Polylysine / Q000378:metabolism; D011485:Protein Binding; D051381:Rats
chemical_list: D002843:Chromatin; D009602:Nitrosamines; D011107:Polylysine; D004247:DNA; D003851:Deoxyribonucleases; D004128:Dimethylnitrosamine