A new direct lead technique for histochemical demonstration of alkaline phosphatase activity.
A lead method for demonstrating alkaline phosphatase is described. The method is based on direct precipitation of lead as lead phosphatase at pH 9.5, the pH optimum of the enzyme. Stable incubation medium was achieved by using tartrate, instead of maleate, as chelating for lead. The method was found to be suitable for visualization of alkaline phosphatase in different types of tissues.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D006651:Histocytochemistry; D006801:Humans; D007962:Leukocytes / Q000201:enzymology; D051379:Mice; D001743:Urinary Bladder / Q000201:enzymology
D000469:Alkaline Phosphatase