A simple method for the quantitation of isozyme patterns.
Isozyme patterns may be analyzed quantitatively, without the use of a densitometer, by performing serial twofold dilutions of a sample to a visual end point. The specific activity (S) of a given dehydrogenase isozyme can be assessed in the presence of other isozymes catalyzing the same reaction, by (1) determining the isozyme titer (T) (defined as mg protein/ml in the last visible band) and (2) applying the formula S = K/T, where K is 1.6 X 10(-3) units/ml in the last visible band. The units/ml (U) in the starting material can be calculated from the equation U = K (2)n-1, where n is the number of the slot producing the last visible band.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D004592:Electrophoresis, Starch Gel / Q000379:methods; D004912:Erythrocytes / Q000032:analysis; D014644:Genetic Variation; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D013045:Species Specificity
D001798:Blood Proteins; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase