title: [Excretion of estriol, estetrol, 16-epi-estriol, 16-keto-estradiol and 16-hydroxyestrone in the 24-hour urine of pregnant women in the last trimester].
abstract: In this publication a method is given which allows simultaneous estimation of estriol, estetrol, 16-epiestriol, 16-ketoestradiol and 16-hydroxyestrone in urine of pregnant women. First conjugates are precipitated with ammoniumsulfate and hydrolyzed. Then the steroids are extracted and converted to azodyes by reacting with the diazonium salt dark blue r. After separation by thin layer chromatography the azodyes are measured by remission analysis with a chromatogramm spectrophotometer. From the data obtained from 66 cases norm groups were set up for the excretion of the steroids in the 3rd trimester of pregnancy. In the last month of pregnancy the average excretion, expressed in % of excreted estriol, is as follows: estetrol 5,7%, 16-epiestriol 3,1%, 16-ketoestradiol 7,0%, 16-hydroxyestrone 5,3%.
mesh_terms: D000328:Adult; D002855:Chromatography, Thin Layer; D004953:Estetrol / Q000652:urine; D004964:Estriol / Q000652:urine; D004967:Estrogens / Q000302:isolation & purification / Q000652:urine*; D004970:Estrone / Q000652:urine; D005260:Female; D006801:Humans; D008722:Methods; D011247:Pregnancy; D011263:Pregnancy Trimester, Third
chemical_list: D004967:Estrogens; D004970:Estrone; D004953:Estetrol; D004964:Estriol
vernacular_title: Die Ausscheidung von Ostriol, Ostetrol, 16-Epi-Ostriol, 16-Keto-Ostradiol und 16-Hydroxy-Ostron im 24-Stundenharn Schwangerer des letzten Trimenons