[The creatine effect on RNA and protein synthesis in growing culture of chick embryo myoblasts].
4hr incubation of the growing culture of chick embrio myoblasts in the presence of 5 mM creatine resulted, regardless of a well-defined lowering of cell membrane permeability to labelled precursors, in: (1) the 1.5-fold induction of 14C-orotic acid incorporation into total cellular RNA; (2) the 1.9-fold stimulation of DNA-dependent RNA-polymerase activity and (3) the preferable, in comparison with total proteins, 14C-leucine incorporation in the myosin heavy chain. The stimulating effect of creatine on RNA-poly-merase deals with a definite enzyme fraction and it is caused by the augmentation of the transcriptional complex enzyme activity. Polyacrilamide gel electrophoresis of total 14C-RNA revealed a relative increase of radioactivity after creatine treatment in both high-molecular pike and the fraction corresponding to 26S RNA, the relative proportion of mRNA containing Poly-A in the composition of total cellular RNA being invariable. The data obtained show that, though creatine as a positive regulator manifestates its effect at different stages of the sucessive chain of reactions leading to muscular protein synthesis, the direct realization of creatine regulatory function is connected with nucleus and is fullfilled at the transcription level.
D000818:Animals; D001755:Blastocyst; D002642:Chick Embryo; D003401:Creatine / Q000494:pharmacology* / Q000502:physiology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D024510:Muscle Development; D009124:Muscle Proteins / Q000096:biosynthesis*; D009132:Muscles / Q000196:embryology / Q000378:metabolism*; D009218:Myosins / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects
D009124:Muscle Proteins; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D009218:Myosins; D003401:Creatine
Vliianie kreatina na sintez RNK i belka v rastusheche i kul'ture mioblastov kurinago embriona