Immunogenicity of inactivated influenza vaccine, purified by adsorption chromatography on porous glas.
A chromatographic process has been developed for a mass purification and concentration of influenza virus. The final virus suspension, purified some 400 times, is characterized by its antigenic properties and protein content. The virus was killed by UV-light and the resulting vaccine tried on 345 people. The immunity level is 8 times higher than the control. In mice which were infected after immunization with a homologous and heterologous influenza virus strains a high protection index was found (10(6) EID50 for homologous and 10(4) EID50 for heterologous strains).
D000327:Adsorption; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002845:Chromatography; D005069:Evaluation Studies as Topic; D006801:Humans; D007114:Immunization; D007252:Influenza Vaccines / Q000302:isolation & purification* / Q000592:standards; D007251:Influenza, Human / Q000517:prevention & control*; D051379:Mice; D009975:Orthomyxoviridae / Q000032:analysis / Q000276:immunology; D014611:Vaccination; D014764:Viral Proteins / Q000032:analysis
D000956:Antigens, Viral; D007252:Influenza Vaccines; D014764:Viral Proteins