[Determination of the size of protein molecules by means of polyacrylamide gel electrophoresis].
A correlation between the relative electrophoretic mobility of 15 proteins and the concentration of polyacrylamide gel was considered. The extrapolation of straight lines to the zero mobility enabled to determine the effective radius of protein molecules and the extrapolation to the zero concentration of the gel was used to estimate the free electrophoretic mobility. Use of the method for study of protein molecules is discussed.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008970:Molecular Weight; D010316:Particle Size; D011487:Protein Conformation; D011506:Proteins
D011506:Proteins
Opredelenie razmera belkovykh molekul s pomoshen'iu zlektroforeza v gele poliakrilamida