Electron-capture gas chromatography of barbituric acids and diphenylhydantoin after pentafluorobenzylation.
Alkylation of barbituric acids and diphenylhydantoin with pentafluorobenzyl bromide is described; the reaction is quantitative in the presence of excess of triethylamine. The electron-capture detector response of the derivatives is high, ranging from 2 to 8 x 10(-17) moles/sec. The derivatives demonstrate excellent peak symmetry and are well suited to quantitative analysis. The structures of the derivatives have been confirmed by mass spectrometry.
D000478:Alkylation; D001463:Barbiturates / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D005464:Fluorobenzenes; D013058:Mass Spectrometry; D010672:Phenytoin / Q000032:analysis*
D001463:Barbiturates; D005464:Fluorobenzenes; D010672:Phenytoin