In vitro radiolabeling procedure which labels the proteins of Newcastle disease virions with carbon-14.
A convenient and gentle procedure was used for in vitro radioisotopic labeling the proteins of Newcastle disease virus. This method, which utilizes [14C]formaldehyde and sodium borohydride, labels all the proteins of purified Newcastle disease virus. Virions labeled in this manner retained biological and biopysical integrity
D000818:Animals; D001894:Borohydrides / Q000378:metabolism; D002250:Carbon Radioisotopes; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005557:Formaldehyde / Q000378:metabolism; D066298:In Vitro Techniques; D007553:Isotope Labeling; D009521:Newcastle Disease / Q000175:diagnosis; D009522:Newcastle disease virus; D014764:Viral Proteins / Q000032:analysis / Q000378:metabolism
D001894:Borohydrides; D002250:Carbon Radioisotopes; D014764:Viral Proteins; D005557:Formaldehyde