Cellular interactions in the control of granulopoiesis.
We have examined several new techniques for studying cellular interactions in the control of granulopoiesis in vitro. Better in vitro growth of mouse bone marrow can be achieved by modifying standard diffusion chamber systems to allow continuous fluid circulation and cell migration. When certain organ fragments are added to such systems, enhanced granulocytic differentiation is observed.
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002465:Cell Movement; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D007962:Leukocytes / Q000166:cytology*; D008722:Methods; D051379:Mice; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology