title: Establishment of two cell lines from embryonic tissue of the tobacco hornworm, Manduca sexta (L).
abstract: Two cell lines were derived from primary cultures of embryonic tissue of the tobacco hornworm, Manduca sexta (L.). The cell lines were maintained on hemolymph-free synthetic insect medium. Cytogenetic and immunoligical identification of the lines were carried out. Techniques for obtaining the line and medium, subculturieng and freezing procedures for long-term storage, and the morphological and growth characteristics are described.
mesh_terms: D002449:Cell Aggregation; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002470:Cell Survival; D002478:Cells, Cultured / Q000648:ultrastructure; D003470:Culture Media; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005615:Freezing; D007621:Karyotyping; D007915:Lepidoptera / Q000166:cytology*; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D003470:Culture Media