title: The effect of hypertonic urea solution on Na+ and K+ transport through the smooth muscle membrane.
abstract: The aim of this work was to examine the effect of a hypertonic solution (Krebs solution + 290 mM urea) on K+ and Na+ transport. The experiments were carried out on the guinea-pig taenia coli preparations using the method of Na-24 and K-24 loading and washout. The efflux curves were analysed by means of the digital computer technique. The following parameters were determined: efflux rate constant k2, influx rate constant k1, intracellular ion concentration C1 ion flux M and permeability P. Any significant difference between PNa/PK ratio in hypertonic urea and isotonic Krebs solutions was found.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003106:Colon / Q000378:metabolism; D006168:Guinea Pigs; D006982:Hypertonic Solutions; D009130:Muscle, Smooth / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D014508:Urea / Q000494:pharmacology*
chemical_list: D006982:Hypertonic Solutions; D014508:Urea; D012964:Sodium; D011188:Potassium