title: Rapid purification of 70S RNA from media of cells producing RNA tumor viruses.
abstract: Media from cells producing RNA tumor viruses, when treated with sodium dodecyl sulfate and polyvinyl sulfate, yield 70S RNA as the major species binding oligo(dT)-cellulose. The procedure described for purifying 70S RNA requires no special equipment and is suitable for rapidly processing large quantities of media or for purifying RNA from commercially avialable virus, with a 5- to 10-fold higher yield than was obtained using existing methods.
mesh_terms: D002460:Cell Line; D002845:Chromatography / Q000379:methods; D003470:Culture Media / Q000032:analysis; D005069:Evaluation Studies as Topic; D009858:Oncogenic Viruses / Q000032:analysis* / Q000254:growth & development; D011145:Polyvinyls; D012328:RNA Viruses / Q000032:analysis* / Q000254:growth & development; D012367:RNA, Viral / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D014779:Virus Replication
chemical_list: D003470:Culture Media; D011145:Polyvinyls; D012367:RNA, Viral; D012967:Sodium Dodecyl Sulfate