title: In vitro growth promotion of rat leukemia L5222 cells in the presence of 2-mercaptoethanol.
abstract: Propagation of the transplantable acute rat leukemia L5222 in vitro was highly dependent on the reducing agent 2-mercaptoethanol (2-Me). Maximum growth promotion was obtained in the simultaneous presence of 1.25-2.5 x 10-4m 2-Me and 30% fetal bovine serum in enriched tissue culture medium NCTC 135. A short exposure to the reducing agent insured growth of the leukemia cells for 5 days, but extended culture necessitated constant presence of the thiol. After 2-8 days of culture, inoculation of the cell suspension produced widespread leukemia in inbred BD IX rats.
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D007942:Leukemia, Experimental / Q000209:etiology; D008623:Mercaptoethanol / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D008623:Mercaptoethanol