[Molecular aspects of the interaction of albumin and tannined erythrocytes in the process of hemosensitization. II. The effect of temperature].
A study was made of a combined influence of the temperature of the medium and of sensitin concentration on the process of interaction of tannin-treated sheep erythrocytes and human serum albumin. On the basis of determination of the number of molecules bound by a single erythrocyte during hemosensitization,, depending on the mentioned conditions, it was revealed that the relative total albumin binding increased with the elevation of temperature. Elevation of temperature also led to the absolute and the relative increase in the stable and a reduction in the loose albumin binding by a single erythrocyte and the growth of sensitivity of the passive hemagglutination test. The role of chemical mechanisms in the erythrocyte albumin loading was demonstrated; this permitted to carry out erythrocyte albumin sensitization at a comparatively high temperature for the purpose of increasing the efficacy of passive hemagglutination test.
D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D007114:Immunization; D008967:Molecular Biology; D011485:Protein Binding; D012044:Regression Analysis; D012709:Serum Albumin / Q000276:immunology*; D013634:Tannins; D013696:Temperature
D012709:Serum Albumin; D013634:Tannins
Molekuliarnye aspekty vzaimodeĭstviia al'bumina i tanizirovannykh éritrotsitov v protsesse gemosensibilizatsii. Soobshchenie II. Vliianie temperatury