[Effect of temperature and concentrations of initial components on synthesis of internucleotide bond catalyzed by pancreatic ribonuclease].
The synthesis of cytidylyu-(3,-5,)-cytidine (CpC) catalyzed by pancreatic ribonuclease at 23 degrees, 0 degrees, and -15 degrees C in Tris-HCl-buffer was compared with that in aqueous propan-2-0. The data obtained show that the increase in the yield of oligonucleotides in aqueous buffer at -15 degrees, observed earlier is rather a result of the concentration change in the reaction mixture caused by the freezing of water than by a temperature fall from 0 to -15 degrees. A 4-fold increase in the initial concentrations of the substrates and ribonuclease with respect to the concentrations used earlier leads to the yield of CC in a homogeneous solution at 0 degrees close to is yield found in the frozen mixture at -15 degrees.
D003597:Cytosine Nucleotides / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D009841:Oligonucleotides / Q000096:biosynthesis*; D009843:Oligoribonucleotides / Q000096:biosynthesis*; D010179:Pancreas / Q000201:enzymology*; D012260:Ribonucleases / Q000378:metabolism*; D013696:Temperature
D003597:Cytosine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012260:Ribonucleases
Vliianie temperatury i kontsentratsil iskhodnykh komponentov na sintez mezhnukleotidnoĭ sviazi, kataliziruemĭ pankreaticheskoĭ ribonukeazoĭ