title: [Effect of proteolysis inhibitors on the incorporation of labelled amino acids into proteins].
abstract: Role of peptide bond breaks in the incorporation of amino acids into proteins in a "protein--amino acid" system is investigated. For this purpose the incorporation of labelled amino acids into trypsin under the inhibition of its autolysis by a specific inhibitor from soybean and epsilon-amino-caproic acid is studied. The trypsin inhibitor from soybean is found to suppress considerably the incorporation of 14C-glycine, 14C-lysine and 14C-methionine into crystal trypsin and not to affect the incorporation of labelled amino acids into chomotrypsin, papain and carboxypeptidase. Epsilon-Aminocaproic acid inhibited 14C-glycine incorporation into crystal trypsin by 40% and did not change its incorporation level into serum albumin. The dependency of amino acid incorporation level into trypsin on the activity of autolysis in the "protein--amino acid" system is demonstrated.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000494:pharmacology*; D014361:Trypsin Inhibitors / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D014361:Trypsin Inhibitors; D014360:Trypsin Inhibitor, Kunitz Soybean
vernacular_title: Vliianie ingibitorov proteoliza na vkliuchenie mechenykh aminokislot v izolirovannye belki