Reconstitution of pig lymphocyte plasma membranes from solubilized components, with particular reference to membrane-associated immunoglobulins.
Plasma membranes of lymphocytes obtained from pig mesenteric lymph nodes were reconstituted after solubilization with bile salts. The proportion by weight of immunoglobulin in the reconstituted membrane was no greater than about 5-10% of that in the original membrane. Possible reasons for the low reincorporation of immunoglobulin are discussed.
D000818:Animals; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D008214:Lymphocytes / Q000648:ultrastructure*; D008854:Microscopy, Electron; D011947:Receptors, Antigen, B-Cell; D012995:Solubility; D013552:Swine
D003902:Detergents; D011947:Receptors, Antigen, B-Cell