Purification of tumor-specific antigens. An overview of the relevance to human colon carcinoma.
Methods which dissociate intramolecular noncovalent bonds have been used to prepare soluble derivatives of cell-surface antigens. Applications of these techniques to human colon carcinoma are underway. Continuous tissue-culture strains derived from primary lesions were developed and shown to be composed of malignant epithelial elements. Parallel data on the preparation and activity of soluble materials in a murine model methylcholanthrene system reveal that although cultured cells are a satisfactory source for antigen extraction, they are poor targets of the immune response. The development of methods to quantitate the biologic activity of colon-specific, soluble materials may provide indicator systems to define the antigenic determinants, to permit purification, and to serve as assays of the efficacy of immunotherapy.
D000230:Adenocarcinoma / Q000276:immunology* / Q000517:prevention & control; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D002272:Carcinoembryonic Antigen; D002460:Cell Line; D003110:Colonic Neoplasms / Q000276:immunology* / Q000517:prevention & control; D006801:Humans; D007111:Immunity, Cellular; D012995:Solubility
D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen