[Isolation of cathepsins D by affinity chromatography].
The paper deals with the isolation of cathepsin D from rat liver, chicken liver and bovine spleen by affinity chromatography. The synthesis of the adsorbent was performed using the competitive inhibitor of cathepsins D and pepsin pepstatin and activated Sepharose. Application of a pepstatin-Sepharose column allows obtaining a highly active and purified enzyme in a short period of time.
D000818:Animals; D002403:Cathepsins / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D002417:Cattle; D002645:Chickens; D002846:Chromatography, Affinity; D008099:Liver / Q000201:enzymology; D010436:Pepstatins; D051381:Rats; D012685:Sepharose; D013154:Spleen / Q000201:enzymology
D010436:Pepstatins; D012685:Sepharose; D002403:Cathepsins
Vydelenie katepsinov gruppy D s pomoshch'iu khromatografii po srodstvu