Thin-layer chromatographic determination of barbiturates and phenytoin in serum and blood.
A method is described for the measurement of blood, serum and/or plasma levels of hexobarbital, phenobarbital, cyclobarbital and phenytoin by ultraviolet reflectance photometry on thin-layer chromatograms. The lowest concentrations measured were 0.3-0.7 mug/ml. The accuracy was similar to that of gas chromatographic procudures. For phenytoin determinations 5-(p-methylphenyl)-5-phenylhydantoin may be used as internal standard. The method has been applied to clinico-pharmacological assays, to the measurement of cyclobarbital elimination in man following a therapeutic dose, and to the study of phenobarbital kinetics in rats using serial blood samples.
D000818:Animals; D001463:Barbiturates / Q000097:blood*; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D006591:Hexobarbital / Q000097:blood; D006801:Humans; D007700:Kinetics; D010634:Phenobarbital / Q000097:blood; D010672:Phenytoin / Q000097:blood*; D051381:Rats
D001463:Barbiturates; D010672:Phenytoin; D006591:Hexobarbital; D010634:Phenobarbital