Comparison of RNA polymerase associated with Newcastle disease virus and a temperature-sensitive mutant of Newcastle disease virus isolated from persistently infected L cells.
An in vitro comparison was made of the RNA polymerase activity associated with Newcastle disease virus (NDVo) and three clones of the temperature-sensitive mutant (NDVpi) isolated from persistently infected L cells. Less polymerase activity was associated with the NDVpi clones. Also, compared to NDVo, an increase in incubation temperature from 32 to 37 or 42 C resulted in a marked decrease in polymerase activity for the temperature-sensitive mutants which coincided with their inability to replicate at 42 C.
D002474:Cell-Free System; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D007700:Kinetics; D007739:L Cells; D009154:Mutation; D009522:Newcastle disease virus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D013696:Temperature; D014779:Virus Replication
D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases