[Dynamics of the amino acid composition of the medium during cultivation of isolated liver and kidney by the controlled perfusion method].
The dynamics of the medium amino acid composition was studied during a 6-hour perfusion of dog kidney and liver by a mixture of autogenic plasma and medium 199 (a ratio of 2 : 3). In comparison to the initial level the amount of histidine during 6-hour cultivation of the kidney was found to increase 2.2 times, that of glutamic acid--1.7 times, and of alanine and lysine--1.6 times. At the same time the amount of arginine, serine and asparagic acid became 3.3 times and of glutamine and threonine--2.5 times lower. With the liver perfusion the level of glutaminic acid became 2.9 times, of alanine--2.3 times, of cystine--2 times and of glycine--1.5 times higher. With the liver perfusion the level of arginine decreased so rapidly that none was found in the medium after a 2-hour perfusion. The described method of amino acid analysis during the cultivation of the organs could serve as means for elaborating and correcting the culture media.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D009924:Organ Culture Techniques
D000596:Amino Acids
Dinamika aminokislotnogo sostava sredy pri kul'tivirovanii izolirovannykh pecheni i pochki metodom upravliaemoĭ perfuzii