[Spectral manifestations of different types of acriflavine binding to DNA in ultraviolet and visible region].
Difference absorption spectra (complex-sum of the initial reagents) are obtained in the visible and longwave UV region for the system of actiflavine and DNA in a number of cases differing in initial and final degrees of DNA filling by the dye, in particular separately for two types of dye binding to DNA. For these binding types conventional absorption spectra are calculated. In the visible region for the first binding type ("strong" binding) red shift of the absorption band is observed; for the second type ("weak" binding) we observed splitting of the band, short wavelength component being highly prevailing, and hypochromism. In the UV region for both binding types the spectra changed in approximately similar way; a slight blue shift and a rather remarkable hypochromism are observed. It is shown that the dye brings the main contribution into the spectral changes in the UV region. If to take into account the spectral properties of molecular aggregates the data obtained are compatible with the intercalation model for "strong" binding and dye stacking on DNA for "weak" binding.
D000166:Acridines; D000167:Acriflavine; D004247:DNA; D009690:Nucleic Acid Conformation; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
D000166:Acridines; D000167:Acriflavine; D004247:DNA
Spektral'nye proiavleniia raznykh tipov sviazi akriflavina s DNK v ul'trafioletrovoĭ i vidimoĭ oblastiakh