[Solubilization of sarcolemma acetylcholinesterase with Triton X-100 and KCl].
It is shown that simultaneous treatment with sarcolemma with triton X-100 (0.1-1.0%) and KCl (0.6-1.2 M) solubilizes about 60% of acetyl cholinesterase, however its specific activity in the intact sarcolemma and in the obtained fractions is the same. A considerable part of lipids (about 90% of phospholipids and 50% of cholesterol) may be removed by treating sarcolemma with 0.1% triton X-100; it may be fixed in the membrane by hydrophoric bonds. Other phospholipids and cholesterol are bound with sarcolemma proteins more strongly and, probably, form the protein-lipid complexes. In the composition of phospholipids strongly bound with sarcolemma proteins a relative content of sphingomyelin increases and that of lysolecithin decreases.
D000110:Acetylcholinesterase / Q000302:isolation & purification*; D000818:Animals; D008055:Lipids / Q000302:isolation & purification; D008854:Microscopy, Electron; D011092:Polyethylene Glycols; D011189:Potassium Chloride; D011817:Rabbits; D012508:Sarcolemma / Q000032:analysis / Q000201:enzymology* / Q000648:ultrastructure
D008055:Lipids; D011092:Polyethylene Glycols; D011189:Potassium Chloride; D000110:Acetylcholinesterase
Solivbilizatsiia atsetilkholinesterazi sarkolemi Tritonom X-100 ta KCl