Y-chromatin positive cells in the smear preparations of the gonad from a XX male.
An ambisexual male infant showed a normal female karyotype by the leukocyte culture technique in two different occasions. Cultured fibroblasts from the right gonad had also a 46, XX complement. They were Y-chromatin negative. To our surprise, Y-chromatin was observed in 96% of the nuclei in smears from the uncultured gonad. It is emphasized that the examination of the uncultured gonad by the quinacrine technique is mandatory in the study of "XX males".
D005347:Fibroblasts / Q000648:ultrastructure; D006066:Gonads / Q000166:cytology; D006801:Humans; D007223:Infant; D009030:Mosaicism; D012728:Sex Chromatin; D012729:Sex Chromosome Aberrations / Q000235:genetics*; D012730:Sex Chromosomes