title: Synthesis of brain-specific acidic proteins in rat and mouse cerebral slices.
abstract: Optimal conditions for incorporation of radioactive amiino acids into protein by slices of brain have been established. Protein synthesis continued for at least 4 h at 35 degrees. The highest incorporation of amino acids into protein was obtained with 20-50 mg wet weight per ml incubated at pH 7.1. After incubation of 500 mg of slices acidic proteins were separated by saltfractionation and electrophoresis on 14% polyacrylamide gel. The most acidic band was shown to contain S-100 protein. Indentification was solubility of the protein in 100% (NH4)2SO4, electrophoretic mobility at pH 8.9, precipitation with specific antibodies, and 2-dimensional electrophoresis in polyacrylamide gel. The protein band was insoluble in 50% methanol and contained between 0.06 and 0.2% of the radioactivity present in the total soluble proteins; while other well separated acidic proteins contained about 10 times the radioactivity of S-100 protein.
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D051381:Rats; D009418:S100 Proteins / Q000096:biosynthesis
chemical_list: D000596:Amino Acids; D009419:Nerve Tissue Proteins; D009418:S100 Proteins