Properties of immobilized flavodoxin from Peptostreptococcus elsdenii. An affinity ligand for the purification of riboflavin 5'-phosphate (FMN) and its analogues.
The small flavoprotein, flavodoxin, isolated from Peptostreptococcus elsdenii, has been covalently coupled to CNBr-activated Sepharose 4B. The immobilized protein replaces ferredoxin as an electron carrier in hydrogen production from dithionite or pyruvate in the presence of ferredoxin-free extracts of P. elsdenii; compared with soluble flavodoxin, its activities in these systems are 13% and 3.5% respectively. Acid treatment reversibly dissociates FMN from the immobilized protein. The dissociation constant of the complex with FMN, determined by fluorimetric titration, is 1.5 (+/- 0.4) nM, and is therefore very little different from that of soluble flavodoxin. Like soluble apoflavodoxin, the immobilized apoprotein is highly specific for flavins with an N-10 side-chain of 5 carbon atoms and a C-5' phosphate group. Approximately half of the flavin impurity in commercial preparations of FMN (12-15% of the total flavin), and similar impurity in synthetic analogues of FMN, is not separated by conventional purification procedures, but it is readily and conveniently removed by affinity chromatography with apoflavodoxin as the immobilized ligand. The immobilized protein is stable for long periods; its capacity for FMN decreases by only 20% after 15 cycles of flavin dissociation and reassociation during several months.
D001426:Bacterial Proteins / Q000378:metabolism; D001665:Binding Sites; D002846:Chromatography, Affinity / Q000379:methods; D005486:Flavin Mononucleotide / Q000031:analogs & derivatives / Q000302:isolation & purification*; D005420:Flavoproteins / Q000378:metabolism; D007700:Kinetics; D010462:Peptostreptococcus / Q000378:metabolism*; D011485:Protein Binding; D012995:Solubility; D013329:Structure-Activity Relationship
D001426:Bacterial Proteins; D005420:Flavoproteins; D005486:Flavin Mononucleotide