title: [Determination of prolactin].
abstract: Prolactin is a pituitary stimulating hormone which is capable of inducing and maintaining lactation. The recent introduction of very sensitive methods of estimating the levels have led to its isolation and identification of its physico-chemical characteristics in the human and also to the physio-pathological study of its secretion. The authors demonstrate a human homologous radio-immune level estimating technique which uses a human anti-prolactin serum obtained from the rabbit human prolactin highly purified with iodine 125 using the lacto-peroxydase method. This radio-immune level estimation constitutes a feasible and satisfactory approach for the base level of prolactin. The spread of results which have been obtained, however, showed that one sole estimation is not sufficient. The authors draw attention to the need to carry out dynamic tests of stimulation or of braking in order to reveal possible abnormalities of prolactin secretion.
mesh_terms: D000818:Animals; D002940:Circadian Rhythm; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D011388:Prolactin / Q000097:blood* / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D007457:Iodine Radioisotopes; D011388:Prolactin
vernacular_title: Dosage de la prolactine