Reversible inhibition of DNA, RNA and protein synthesis in human cells by lead chloride.
An acute intoxication by lead chloride (conc. 2.5 - 10(-4)M) produces a temporary reduction of macromolecular syntheses in HeLa cells growing asynchronously. The reduction is similar for DNA, RNA and proteins and differs only in the intensity. After a one-day intoxication, if the cells are put back in a fresh medium, the syntheses return to normal within 10 h. The histochemical sulphide-silver method shows that lead is present in the cells during the inhibition.
D002455:Cell Division / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D003864:Depression, Chemical; D006367:HeLa Cells / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D007854:Lead / Q000378:metabolism / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*
D007854:Lead; D012313:RNA; D004247:DNA