Adenylate cyclase stimulation by trypsin.
Adenylate cyclase activity of a rat embryo fibroblast cell line (F111) is markedly increased by brief treatment with 1:300 trypsin. The degree of stimulation depends upon the length of time the cells are treated and the concentration of trypsin. Crystalline trypsin produced a stimulation similar to that obtained with 1:300 trypsin. Further, the addition of soybean trypsin inhibitor blocked the stimulation of adenylate cyclase by 1:300 trypsin. Trypsin-treated adenylate cyclase responds to PGE1, but there is no increase over that of untreated enzyme. This result and the increase in fluoride-stimulated levels of activity suggest that the trypsin is acting upon the catalytic unit of the enzyme.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D002460:Cell Line; D004789:Enzyme Activation; D005459:Fluorides / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology*; D014361:Trypsin Inhibitors
D011458:Prostaglandins E; D014361:Trypsin Inhibitors; D014357:Trypsin; D000262:Adenylyl Cyclases; D005459:Fluorides