[Isolation and physico-chemical properties of oligosaccharides of human milk].
From a non-dialysable fraction of human milk the authors have isolated 30 oligosaccharides by combining anion exchange and paper chromatography. These oligosaccharides (MW 998 to 3113) contain D (+)-galactose, L (-)-fucose, N-acetyl-D (+)-glucosamine, N-acetyl-D (-)-neuraminic acid in variable proportions and one single D (+)-glucose residue in terminal reducing position. The characteristics of the oligosaccharides are those of the so-called "Polonovski and Lespagnol gynolactose". These sugars do not originate from glycoproteins nor glycolipids. The authors suggest that the biosynthesis of human milk oligosaccharides is due to an activation of glycosyltransferases related to blood group substances, induced by lactose. They base this hypothesis on the fact that, as demonstrated by Strecker and Montreuil, spontaneous or glucose, galactose and lactose induced meliturias are accompagnied by an important urinary excretion of blood group substance related oligosaccharides.
D000117:Acetylglucosamine / Q000032:analysis; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D005260:Female; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006801:Humans; D008895:Milk, Human / Q000032:analysis*; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification; D011247:Pregnancy; D012794:Sialic Acids / Q000032:analysis
D009844:Oligosaccharides; D012794:Sialic Acids; D005643:Fucose; D005947:Glucose; D000117:Acetylglucosamine; D005690:Galactose
Isolement et étude des propriétés physico-chimiques d'oligosaccharides du lait de femme