Studies on the action of nerve growth factor. II. Neurotubule protein levels during neurite outgrowth.
The neurotubule protein content of chick embryo 8-day dorsal root and 14-day sympathetic ganglia, induced to extend neurites in the presence of Nerve Growth Factor, was determined by the time-decay colchicine binding assay procedure and by two independent polyacrylamide gel electrophoresis systems. The initial level of neurotubule protein in dorsal root ganglia was approximately 16% of the total soluble protein. This value was constant during Nerve Growth Factor-mediated neurite outgrowth. The initial level of neurotubule protein in sympathetic ganglia was also approximately 16%, and was unchanged during neurite outgrowth. In addition, C1300 mouse neuroblastoma cells, induced to extend neurites in 0.1% serum, also did not exhibit a change in neurotubule protein concentration, which remained approximately 9% of the total soluble protein.
D000818:Animals; D001369:Axons; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D005725:Ganglia, Autonomic / Q000378:metabolism; D005727:Ganglia, Spinal / Q000378:metabolism; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure
D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins