Purification and characterization of a hemagglutinin from Arachis hypogeae.
A. hypogaea hemagglutinin was purified by ammonium sulfate fractionation and Sepharose 6 B column chromatography. The homogeneity of the purified hemagglutinin was ascertained by ultracentrifugal analysis and polyacrylamide gel electrophoresis. It has a molecular weight of 106.500 and is a tetramer of a subunit with a molecular weight of 27.000. The purified hemagglutinin agglutinated neuraminidase-treated human erythrocytes regardless of their ABO group type, but did not agglutinate intact erythrocytes. In hapten inhibition assays with simple sugars, the so-called Mäkelä's group 2 sugars, which bear the same configuration of hydroxy groups at C-3 and C-4 as D-galactopyranose, were inhibitors for this hemagglutinin. It does not contain any carbohydrate, in contrast to most phytohemagglutinins except concanavalin A and wheat germ agglutinin.
D000017:ABO Blood-Group System; D000373:Agglutinins / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D002241:Carbohydrates / Q000032:analysis; D002462:Cell Membrane; D004912:Erythrocytes; D006020:Glycopeptides / Q000097:blood; D006023:Glycoproteins / Q000097:blood; D006388:Hemagglutinins / Q000032:analysis*; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight; D009439:Neuraminidase; D011485:Protein Binding; D012639:Seeds / Q000032:analysis*
D000017:ABO Blood-Group System; D000373:Agglutinins; D000596:Amino Acids; D002241:Carbohydrates; D006020:Glycopeptides; D006023:Glycoproteins; D006388:Hemagglutinins; D046911:Macromolecular Substances; D009439:Neuraminidase