Concurrent development of resistance to 6-azauridine and adenosine in a mouse cell line.
A variant of the hypoxanthine-guanine phosphoribosyltransferase deficient, and adenine phosphoribosyltransferase deficient mouse resistant to 6-azauridine. These cells are not only resistant to 6-azauridine (5 X 10(-4) M), but also to adenosine (10(-3) M). Resistance persists indefinitely even in the absence of both compounds. The resistant cells are killed by 5-fluorouridine (10(-6) M), indicating that the part of the salvage pathway for pyrimidine ribonucleotide biosynthesis which is relevant to the action of 6-azauridine is intact. The heritable change producing concurrent resistance to 6-azauridine and adenosine probably involves the de novo pyrimidine biosynthetic pathway.
D000241:Adenosine / Q000494:pharmacology*; D001380:Azauridine / Q000494:pharmacology*; D001769:Blood; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects; D003470:Culture Media; D004351:Drug Resistance; D007621:Karyotyping; D007928:Lethal Dose 50; D010641:Phenotype; D014529:Uridine / Q000031:analogs & derivatives
D003470:Culture Media; D001380:Azauridine; D000241:Adenosine; D014529:Uridine