[Improvement in the granulocyte harvest from normal donors by means of a combination of "continuous flow" centrifugation and filter leukapheresis].
In 27 healthy donors a total of 30 leukaphreses were performed with the Aminco cellseparator. Pretreatment by steroids, anticoagulation by heparin and addition of fluid gelatin to the input line was performed in all runs. The combination of continuous flow centrifugation and filtration leukapheresis in 9 of the 30 runs resulted in highly significant increase in the yield of neutrophil granulocytes to a mean of 59.6x10(9) as compared with a mean of 30.8x10(9) obtained the conventional continuous-flow centrifugation alone. This significance is valid for the total yield of granulocytes as well as for the yield per hour of run or per liter of blood processed. No adverse reaction occurred.
D001803:Blood Transfusion; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D006098:Granulocytes; D006801:Humans; D007962:Leukocytes; D010956:Plasmapheresis
Verbesserung der Granulozytengewinnung von Normalspendern durch Kombination von "continuous-flow"-Zentrifugation und Filterleukapherese