Preparation of superior thin-layer chromatograms for quantitative in situ densitometry by nonequilibrated development. I. Trisulfapyrimidines: sulfadiazine, sulfamerazine, and sulfamethazine.
A method of obtaining controlled nonequilibrated horizontal plate development by employing a developing system open to room atmosphere is described and compared with other plate-developing techniques. Nonequilibrated plate development produced spot configurations with the precise physical outline and densitometric distribution needed for optical scanning.
D002855:Chromatography, Thin Layer / Q000295:instrumentation; D003720:Densitometry; D013411:Sulfadiazine / Q000032:analysis; D013416:Sulfamerazine / Q000032:analysis; D013418:Sulfamethazine / Q000032:analysis; D013449:Sulfonamides / Q000032:analysis*
D013449:Sulfonamides; D013411:Sulfadiazine; D013418:Sulfamethazine; D013416:Sulfamerazine