title: Rapid regeneration of the dermal-epidermal junction after partial separation by vacuum: an electron microscopic study.
abstract: A process of rapid repair of dermal-epidermal adherence, as found in experiments with interrupted suction, was investigated electron microscopically. Biopsies in different stages of the process of suction blister formation and of the repair process were studied. Results show that suction blister formation occurs by successive detachment of hemidesmosomes from the basement membrane, and that, after partial separation of the epidermis from the dermis, a rapid regeneration of the dermal-epidermal junction takes place. This regeneration process apparently consists of two steps: realignment of basal cells to the basement membrane accompanied by autophagocytosis of detached hemidesmosomes, and de novo formation of hemidesmosomes. The time required for this structural regeneration corresponds well with the speed of the functional repair of dermal-epidermal adherence measured with the technique of interrupted suction.
mesh_terms: D000328:Adult; D001768:Blister / Q000473:pathology* / Q000503:physiopathology; D003896:Desmosomes / Q000648:ultrastructure; D004487:Edema; D006801:Humans; D007365:Intercellular Junctions / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D012038:Regeneration; D012867:Skin / Q000293:injuries* / Q000503:physiopathology / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure