title: Ultrastructure of the milk fat globule membrane with and without triglyceride.
abstract: The primary milk fat globule membrane (MFGM) around freshly secreted milk fat globules consists of a unit membrane separated from the triglyceride core by a dense material. This dense material may widen to include cytoplasmic organelles or may form small blebs. Preincubation and fixation of the globules at temperatures between 4 degrees C and 60 degrees C has no effect on the width or appearance of the dense material. Isolated MFGM profiles show structures identical to those found on intact globules. The dense material on the isolated MFGM profiles is unaffected by extractions which remove essentially all the triglyceride present in the pellets of MFGM. The structure of the primary MFGM is therefore independent of any triglyceride content and the earlier suggestions that the dark material represented a triglyceride layer of high melting point adsorped during cooling of the globules after milking are not supported by the work described in this paper.
mesh_terms: D000818:Animals; D002417:Cattle; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D008055:Lipids / Q000032:analysis; D008566:Membranes / Q000648:ultrastructure; D008892:Milk / Q000032:analysis*; D013696:Temperature; D014280:Triglycerides / Q000032:analysis*
chemical_list: D005227:Fatty Acids; D008055:Lipids; D014280:Triglycerides