The case for colorimetric two-point stopped-enzyme-activity determinations exhibiting zero-order reactions.
A careful assessment is made of the relative merits of the two common approaches to enzyme determinations: the so-called kinetic and the two-point colorimetric. A plea is made for a pluralistic approach to the selection of enzyme methods based on a full understanding of the modalities of each procedure. A rational approach to clinical enzymology demands an intellignet choice of methods, irrespective of mode, that will best suit a particular laboratory situation.
D003124:Colorimetry / Q000379:methods; D004798:Enzymes / Q000032:analysis*; D007700:Kinetics; D008722:Methods; D013053:Spectrophotometry / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D004798:Enzymes