A nuclear magnetic resonance study of the metal binding sites in bacitracin.
Carbon-13 nuclear magnetic resonance spectroscopy has been used to identify sites in bacitracin which bind Cu2+ and Mn2+. Results are presented which implicate the free carboxyl groups of the aspartic and glutamic acid residues and the imidazole ring of the histidine residue as metal complexation sites between pH 6 and 8. Evidence is presented which also indicates that the thiazoline ring of bacitracin binds Mn2+. Bacitracin does not bind Cu2+ or Mn2+ at pH values of 2.5 or less.
D001414:Bacitracin; D001665:Binding Sites; D003300:Copper; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese
D001414:Bacitracin; D008345:Manganese; D003300:Copper