Characteration of RNA synthesized during germination of Blastocladia ramosa Zoospores.
The synthesis of RNA was studied during the synchronous germination of Blastocladia ramosa zoospores. Comparison of RNA synthesis during germination of B. ramosa and Blastocladiella emersonii zoospores revealed that B. ramosa has a longer lag time before RNA synthesis is initiated and, in addition, the rate of RNA synthesis is ten-fold lower in B. ramosa. Zoospores of B. ramosa were shown to contain pre-formed messenger RNA but this messenger RNA directs only a portion of the protein synthesis which occurs during early germination. The conclusion that the remainder of the protein synthetic activity of the germinating spores is due to new message synthesis was supported by demonstrating that the timing of the initation of protein synthesis on new messages correlates with the time RNA synthesis is initiated. New message synthesis was also demonstrated by the incorporation of label into RNA which contains a poly (A) fragment. Synthesis of all classes of RNA including ribosomal, messenger, and transfer RNA was shown to be initiated at the same time. The implications of this observation are discussed.
D001754:Blastocladiella / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D005656:Fungal Proteins / Q000096:biosynthesis; D005658:Fungi / Q000254:growth & development / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis; D013172:Spores, Fungal / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors
D005656:Fungal Proteins; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D003609:Dactinomycin; D011061:Poly A; D012313:RNA; D012343:RNA, Transfer