title: Studies on chromatin. III. v-Bodies and free DNA in chromatin lacking histone H1.
abstract: Chromatin lacking histone H1 was found by electron microscopy to contain 'beaded' deoxyribonucleoprotein fibers. Adjacent beads are connected with each other by threads having a DNA-like appearance. At least some of threads are shown to be free DNA stretches. Average length and the content of free DNA stretches in histone H1-depleted chromatin depends on the ionic conditions of the medium. The appearance of individual beads is similar to that of chromatin subunits or v-bodies [1] in the original chromatin. Thus, in agreement with the X-ray data [2], histone H1 apparently is not required for maintenance of a compact state of DNA in chromatin subunits.
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D003852:Deoxyribonucleoproteins / Q000032:analysis; D006657:Histones / Q000032:analysis*; D051379:Mice; D008970:Molecular Weight; D009690:Nucleic Acid Conformation
chemical_list: D002843:Chromatin; D004273:DNA, Neoplasm; D003852:Deoxyribonucleoproteins; D006657:Histones