11 beta-Hydroxylase with affinity to C-21-Deoxysteroids from ovaries of patients with polycystic ovary syndrome.
When ovarian mitochondria from a patient with polycystic ovary syndrome (POS) were incubated with [7-3H]17alpha-hydroxypregnenolone and [4-14C]17alpha-hydroxyprogesterone, 11beta-hydroxylated metabolites were obtained. When the same mitochondrial preparation was incubated with [7-3H]17alpha-hydroxypregnenolone and [4-14C]11-deoxycortisol no 11beta-hydroxylated derivatives of 11-deoxycortisol were found. These results are compatible with and support the conclusion that the ovary of POS patients who excrete pregnanetriolone contains an 11beta-hydroxylase capable of hydroxylating C-21-deoxysteroids but not C-21-hydroxysteroids.
D006907:17-alpha-Hydroxypregnenolone / Q000378:metabolism; D003350:Cortodoxone / Q000378:metabolism; D005260:Female; D006801:Humans; D006908:Hydroxyprogesterones / Q000378:metabolism; D006914:Hydroxysteroids / Q000378:metabolism*; D066298:In Vitro Techniques; D008928:Mitochondria / Q000201:enzymology; D010053:Ovary / Q000201:enzymology* / Q000648:ultrastructure; D011085:Polycystic Ovary Syndrome / Q000201:enzymology*; D013250:Steroid Hydroxylases / Q000378:metabolism*
D006908:Hydroxyprogesterones; D006914:Hydroxysteroids; D006907:17-alpha-Hydroxypregnenolone; D013250:Steroid Hydroxylases; D003350:Cortodoxone