The application of radiolabelled HL-A antibodies in the detection of (soluble) transplantation antigens: an alternative for the microcytotoxicity test.
The use of radiolabelled anti HL-A antibody preparations is described. Absorption by platelets, followed by acid elution, fractionation of the eluate by column chromatography, radiolabelling and a second absorption--elution step with platelets yielded specific preparations, irrespective of original cytotoxic titres. The binding of these radiolabelled antibodies to lymphocytes could be inhibited by soluble antigens present in normal plasma or obtained from spleen cells by papain treatment. As a method for the detection of soluble transplantation antigens the use of radiolabelled antibodies appeared as sensitive as the microcytotoxicity test.
D000327:Adsorption; D000918:Antibody Specificity; D001667:Binding, Competitive; D002700:Chloramines / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D007457:Iodine Radioisotopes; D007518:Isoantibodies / Q000302:isolation & purification; D007553:Isotope Labeling
D002700:Chloramines; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007457:Iodine Radioisotopes; D007518:Isoantibodies