Cellular energy dependent agglutination of rat ascites tumor cells mediated by concanavalin A and Ricinus communis agglutinin.
Effect of various metabolic inhibitors on the agglutination of rat ascites tumor cells mediated by concanavalin A and Ricinus communis agglutinin was studied using a quantitative assay method for agglutination in which turbidity of cell suspension is measured. Cell agglutination was inhibited by low temperature, cytochalasin B and inhibitors of energy generating systems without affecting lectin binding, and agglutination was not affected by hydroxyurea, actinomycin D or cycloheximide. The inhibitors of energy generating systems decreased the cellular ATP level and inhibited macromolecular synthesis under the conditions where they inhibited the agglutinations. In contrast, cytochalasin B did not depress the cellular ATP level nor inhibit RNA and protein syntheses. These results suggest that the agglutination is associated with cellular energy dependent processes other than macromolecular synthesis; probably with some cellular surface movements participated by microfilament activity.
D000371:Agglutination; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004140:Dinitrophenols / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D037102:Lectins / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009374:Neoplasms, Experimental / Q000503:physiopathology*; D037121:Plant Lectins; D010947:Plants, Toxic; D012334:RNA, Neoplasm / Q000096:biosynthesis; D051381:Rats; D012278:Ricinus; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D004273:DNA, Neoplasm; D004140:Dinitrophenols; D037102:Lectins; D009363:Neoplasm Proteins; D037121:Plant Lectins; D012334:RNA, Neoplasm; D003208:Concanavalin A; D003571:Cytochalasin B; D007930:Leucine; D013936:Thymidine; D014529:Uridine