title: Studies on chromatin. V. A model for the structure of chromatin subunit.
abstract: A new model for the fine structure of the chromatin subunit (or 'nucleosome') is proposed. The model is based on previous experimental findings [1-14] and on two new suggestions, namely: (1) Eight histones form a toroidal-shaped histone coe of nucleosome and are arranged in the following ciruclar sequence: (see article). (2) DNA is 'kinked' around a toroidal-shaped histone core in a 'solenoid-like' mode, each kink occurring every 10 base pairs along DNA. The electron microscopic evidence for a toroidal shape of the nucleosome is described in the preceding paper [13]. The possibility of the existence of kinks in the DNA double helix was considered recently by Crick and Klug [14]. The proposed model of the nucleosome, being more detailed than earlier models permits us to explain in direct structural terms the yet unordered set of data bearing on the pattern of histone-histone interactions in chromatin, the results of a mild deoxyribonuclease digestion of DNA within the nucleosomal particle and also the quantitative data on the unwinding of the DNA duplex upon formation of the nucleosome.
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002843:Chromatin / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000032:analysis; D003852:Deoxyribonucleoproteins / Q000032:analysis; D006657:Histones / Q000032:analysis; D051379:Mice; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins; D011487:Protein Conformation
chemical_list: D002843:Chromatin; D004273:DNA, Neoplasm; D003852:Deoxyribonucleoproteins; D006657:Histones; D009698:Nucleoproteins