N6-(delta2-isopentenyl)adenosine, an inhibitor of cellular transport of uridine and cytidine.
N6-(delta2-Isopentenyl)adenosine (IPAR) inhibited severely the incorporation of uridine and cytidine into S-180 cells in culture. When IPAR and the nucleosides were simultaneously present in the medium the inhibition was competitive (Ki 3.4 muM) and indicated inhibition of transport. However, the inhibition occurred even in the absence of extracellular IPAR if the cells had been preincubated with IPAR. Since 5'-IPAMP was the product which accumulated in large quantities in S-180 cells when incubated with IPAR, the effects of this AMP analog of the intracellular metabolism of uridine had to be considered. No direct correlation between the amount of intracellular IPAMP and the degree of inhibition of uridine utilization was observed and the relative distribution of uridine nucleotides in the acid soluble pool of the cells was unaltered in cells treated with IPAR. Also, IPAMP was not an inhibitor of uridine kinase in a cell free system nor was the activity of this enzyme affected by treatment of cells with IPAR. In addition, a profound inhibition of uridine utilization was also observed in a resistant subline of S-180 cells, which is unable to form IPAMP. These data suggest that IPAMP was not the inhibitory agent. Furthermore, the observation that the inhibition in both sensitive and resistant cells was caused even by a 15-second exposure to 100 muM IPAR, followed by rinsing, suggests that IPAR itself is the effective agent. It is concluded that IPAR exerts its inhibitory effect on uridine and cytidine utilization by becoming lodged in the cell membrane and thereby preventing the passage of these nucleosides into the cells. It is also shown that the inhibition of uridine and cytidine utilization by IPAR and by other potent nucleoside uptake inhibitors is unrelated to inhibition of growth or RNA-synthesis when the cells do not depend on an extracellular source of a nucleoside for growth.
D000241:Adenosine / Q000031:analogs & derivatives*; D000249:Adenosine Monophosphate / Q000031:analogs & derivatives; D001667:Binding, Competitive; D001693:Biological Transport, Active / Q000187:drug effects; D002460:Cell Line; D003562:Cytidine / Q000378:metabolism*; D003864:Depression, Chemical; D007541:Isopentenyladenosine / Q000378:metabolism / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D014529:Uridine / Q000378:metabolism*; D014541:Uridine Kinase / Q000378:metabolism
D000249:Adenosine Monophosphate; D003562:Cytidine; D012313:RNA; D007541:Isopentenyladenosine; D014541:Uridine Kinase; D000241:Adenosine; D014529:Uridine