The influence of acridine dyes and caffeine on recovery from ultraviolet damage in Eudorina elegans.
Caffeine and the acridine dyes, acridine orange and acriflavine, were used to examine the repair potential in Eudorina elegans following ultraviolet irradiation. Acridines blocked photoreactivation primarily as a result of absorption of photoreactivating wavelengths, but acridines did not influence dark survival. Therefore, an acridine-sensitive excision-resynthesis-repair process is absent in Eudorina. Caffeine decreased both dark and light survival, the latter only after relatively high doses of ultraviolet light were used for inactivation. The caffeine-sensitive repair process appears to function most actively when the organisms are engaged in DNA synthesis, indicating that a postreplication-repair system exists in Eudorina. However, the data suggest that a repair system not associated with the DNA synthetic phases may also exist.
D000166:Acridines / Q000494:pharmacology*; D000167:Acriflavine / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000187:drug effects*; D003624:Darkness; D056890:Eukaryota / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D008027:Light; D011830:Radiation Effects; D014466:Ultraviolet Rays
D000166:Acridines; D000167:Acriflavine; D002110:Caffeine; D004247:DNA