Increased lysosomal hydrolase activity in kidney and brain from the vitamin B6-deficient developing rat.
Between 7 and 9 postnatal days, the kidney, spleen heart, and liver from vitamin B6-deficient young either plateaued and failed to gain weight or lost weight, whereas brain continued to gain weight but at a more reduced rate than normal. The level of cofactor in kidney of experimental animals was below that of controls at all time points studied. alpha-L-Fucosidase activity was already moderately increased in deficient kidney at 3 postnatal days and remained elevated throughout the time course. beta-D-galactosidase, acid phosphatase, and hexosaminidases A and B in vitamin B6-deficient young all showed moderate increases in activities after 9 days, at one or more time points. In brain, alpha-L-fucosidase activity was increased somewhat throughout the time course, but changes in beta-D-glucuronidase, beta-D-galactosidase, alpha-D-mannosidase, acid phosphatase, and hexosaminidases A and B did not occur until after 7 postnatal days. When weights of kidney and brain were both normal, there was a marked elevation in alpha-L-fucosidase activity in these organs suggesting that this increase in enzyme activity may be a specific effect of vitamin B6 deficiency. Elevated alpha-L-fucosidase activity in kidhney may reflect alterations in fucose-containing glycosubstances of glomerular basement membranes.
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001806:Blood Urea Nitrogen; D001921:Brain / Q000201:enzymology*; D005260:Female; D005696:Galactosidases / Q000032:analysis; D005966:Glucuronidase / Q000032:analysis; D006128:Growth; D006596:Hexosaminidases / Q000032:analysis; D006867:Hydrolases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D008361:Mannosidases / Q000032:analysis; D009206:Myocardium / Q000201:enzymology; D009929:Organ Size; D011247:Pregnancy; D011732:Pyridoxal Phosphate / Q000032:analysis; D011736:Pyridoxine / Q000032:analysis; D051381:Rats; D013154:Spleen / Q000201:enzymology; D026681:Vitamin B 6 Deficiency / Q000201:enzymology*; D005644:alpha-L-Fucosidase / Q000032:analysis
D011732:Pyridoxal Phosphate; D006867:Hydrolases; D000135:Acid Phosphatase; D005696:Galactosidases; D006596:Hexosaminidases; D008361:Mannosidases; D005966:Glucuronidase; D005644:alpha-L-Fucosidase; D011736:Pyridoxine