[New possibilities for demonstration of some biogenous amines in the adrenal by X-ray microanalysis (author's transl)].
Technical possibilities for the histochemical demonstration of some biogenous amines with the scanning electron-microscope and X-ray microanalysis were shown by using argentaffine and chromaffine reactions. Objects were adrenals of adult minipigs. Beside morphologic studies the amounts of silver or chromium can be determined semiquantitatively by the intensity of the specific X-radiate from "sum up-analysis" out of punctual areas. Furthermore the high amine concentrations in the adrenal medulla permit the performance of "line-analysis" and of "element-distribution-pictures" for silver or chromium. At the points chosen for analysis the concentration of epinephrine + norepinephrine is six times as high in the adrenal medulla as in the cortex; for norepinephrine alone it is only three times higher. Line-analysis shows that the concentration of katecholamines in the cortex is very low in the periphery and high near the medulla. The chromaffine reaction for serotonin leads to a distinct chromium peak in the medulla with a distribution quotient of 4.6 : 1 versus the cortex showing that there must be remarkable amounts of serotonin in the adrenal medulla, inspite of the fact that serotonin cannot by synthetized there from its natural precursor.
D000302:Adrenal Cortex / Q000032:analysis; D000311:Adrenal Glands / Q000032:analysis*; D000313:Adrenal Medulla / Q000032:analysis; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002857:Chromium; D004298:Dopamine / Q000032:analysis; D004577:Electron Probe Microanalysis / Q000379:methods; D004837:Epinephrine / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D009638:Norepinephrine / Q000032:analysis; D012701:Serotonin / Q000032:analysis; D012834:Silver; D013552:Swine
D001679:Biogenic Amines; D002857:Chromium; D012701:Serotonin; D012834:Silver; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine
Neue Möglichkeiten zum Nachweis verschiedener biogener Amine in der Nebenniere mittels Röntgenmikroanalyse