Purification of growth-promoting peptides and proteins, and of histones, by high pressure silica gel chromatography.
A rapid method for the purification of histones and a variety of growth-promoting proteins and peptides by chromatography on silica gel has been developed. The isolation of the growth-promoting components of serum has been hampered by excessive losses associated with the use of water-based purification mens in acidic methanol-H2O solutions (eg. insulin, albumin, the somatomedins) provides a basis for purification on high-pressure silica gel columns, while peptides and histones can be purified in similar solvents. After column chromatography, the solvent is removed by flash-evaporation, or the protein may be precipitated directly from the solvent by neutralization of the pH and the addition of ethanol. The retention of biological activity (eg. somatomedin-C binding to insulin receptors and cell-growth stimulation) and recovery are excellent.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006657:Histones / Q000302:isolation & purification*; D007328:Insulin / Q000097:blood; D010455:Peptides / Q000097:blood / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*; D013002:Somatomedins / Q000302:isolation & purification*
D006657:Histones; D007328:Insulin; D010455:Peptides; D011506:Proteins; D013002:Somatomedins