Purkinje fibre glycogen. A morphologic and biochemical study of glycogen particles isolated from the cow conducting system.
Glycogen from the cow conducting system was extracted by crude and mild methods. For comparison similar extractions were also performed on cow ordinary ventricular tissue. Glycogen particles from the conducting system, isolated by a mild method, were characterized by a low molecular weight (3-5 X 10(6)) and small dimensions (average diameter about 30 nm). 3.5-7% protein was firmly bound to the glycogen. The glycogen, based on spectrophotometric analysis, appeared to be in a native state. Glycogen as a polysaccharide-protein complex can thus be obtained from the cow conducting system and is proposed to be useful for analysis of the structure and function of glycogen in the conducting system.
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D006003:Glycogen / Q000032:analysis* / Q000302:isolation & purification; D006329:Heart Conduction System / Q000032:analysis*; D006651:Histocytochemistry; D008722:Methods; D008970:Molecular Weight; D011690:Purkinje Fibers / Q000032:analysis*; D013053:Spectrophotometry
D006003:Glycogen