Detection of virus-like particles in the coremia of Penicillium claviforme.
A double-stranded ribonucleic acid (dsRNA) was isolated from coremial extracts of wild type P. claviforme, by methylated-albumin kieselguhr chromatography. Differential centrifugations of the coremial extracts from WT and Sh mutant strains yielded two classes of virus-like particles (VLP), of dimensions 25-30 nm, and 50-70 nm. The possible ecological significance of fungal viruses is discussed.
D009154:Mutation; D010407:Penicillium / Q000648:ultrastructure; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D014780:Viruses / Q000302:isolation & purification* / Q000648:ultrastructure
D012367:RNA, Viral