title: Thin-layer chromatographic screening and confirmation of basic drugs of abuse in urine.
abstract: Thin-layer chromatographic procedures are presented for the positive identification of methodone, primary metabolite of methodone (2-ethylidine-1,5-dimethyl-3,3-diphenylpyrrolidine), propoxyphene, norpropoxyphene, cocaine, benzoylecgonine, methoqualone, and phencyclidine from urine specimens. Initial screening of specimens is done by developing plates in ethyl acetate-methanol-diethylamine (90:10:1.6). Samples screened positive are confirmed in methylene chloride-methyl ethyl ketone-concentrated ammonium hydroxide (74:25:0.8), depending on the drug(s) indicated by the screening procedure. The method is quite sensitive, detecting most of the listed drugs at levels of 1.0 mug/ml or less.
mesh_terms: D002855:Chromatography, Thin Layer; D003042:Cocaine / Q000031:analogs & derivatives / Q000652:urine; D011431:Dextropropoxyphene / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D008403:Mass Screening; D008691:Methadone / Q000652:urine; D008702:Methaqualone / Q000652:urine; D008968:Molecular Conformation; D011619:Psychotropic Drugs / Q000652:urine*; D012997:Solvents; D019966:Substance-Related Disorders / Q000652:urine*
chemical_list: D011619:Psychotropic Drugs; D012997:Solvents; D008702:Methaqualone; D003042:Cocaine; D011431:Dextropropoxyphene; D008691:Methadone