Sterols of scallop. I. Application of hydrophobic sephadex derivatives to the resolution of a complex mixture of marine sterols.
Column chromatography on a hydroxyalkoxypropyl derivative of Sephadex LH-20 and on Anasil B has been applied to the resolution of complex marine sterol mixture in combination with argentation thin-layer chromatography and gas chromatography. This approach permits isolation in quantity of individual sterols from a complex mixture and separation of sterol mixtures that were not resolved without the modified Sephadex step. Seventeen sterols were detected in the scallop Placopecten magellanicus. 24-Methyl-cholesterol, 24-ethyl-cholesterol, 24-methyl-22-dehydrocholesterol and 24-ethyl-22-dehydrocholesterol, i.e. sterols whose configuration at C-24 had not been definitively established, were isolated in sufficient quantities for further study by nuclear magnetic resonance spectroscopy.
D000818:Animals; D002784:Cholesterol / Q000031:analogs & derivatives; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D008745:Methylation; D008974:Mollusca / Q000378:metabolism*; D013261:Sterols / Q000302:isolation & purification*
D013261:Sterols; D002784:Cholesterol