title: Effects of cortisol on serially propogated fibroblast cell cultures derived from the rabbit retal lung and skin.
abstract: Cortisol affects the growth of serially propogated, fibroblast cell cultures derived from the rabbit fetal lung in a manner which is dependent upon the gestational age of the material used: early in gestation (20 days), the hormone (10(-7)-10(-5) M) stimulates [6-3H]thymidine incorporation into DNA, while in late gestation (28 days), cortisol (10(-7) and 10(-6) M) inhibits this process. Cultures derived from the rabbit fetal skin are inhibited by cortisol (10(-5) M) at both gestational ages. Fibroblasts derived from lung, but not from skin, efficiently convert cortisone to cortisol and this activity increases with advancing gestation. Cortisol does not affect the incorporation of [3H]choline into lecithin by confluent cultures of any of the fibroblast types studied.
mesh_terms: D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D003348:Cortisone / Q000378:metabolism; D003470:Culture Media; D005260:Female; D005333:Fetus / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology*; D008168:Lung / Q000196:embryology*; D010713:Phosphatidylcholines / Q000096:biosynthesis; D011247:Pregnancy; D011817:Rabbits; D012867:Skin / Q000196:embryology*
chemical_list: D003470:Culture Media; D010713:Phosphatidylcholines; D002794:Choline; D003348:Cortisone; D006854:Hydrocortisone