[Preparation and kinetic properties of immobilized benzyl penicillin acylase].
An active insoluble preparation of immobilized benzyl penicillin acylase (IBA) EC 3.5.1.11 has been obtained by its entrapping into polyacrylamide gel lattice. Due to immobilization the preparation maintains up to 87% of its initial activity. The kinetics of IBA at low substrate concentrations obeys the Michaelis-Menten law; however, the apparent KM value decreases and the temperature optimum elevates. The inhibition by the reaction products--6-aminopenicillanic acid and phenylacetic acid--has been found to be 4.3 mM. The resultant IBA preparation proves to be suitable for hydrolysis of 5% benzyl penicillin solutions.
D000581:Amidohydrolases / Q000378:metabolism*; D001665:Binding Sites; D007700:Kinetics; D008433:Mathematics; D010399:Penicillin Amidase / Q000302:isolation & purification / Q000378:metabolism*; D010400:Penicillin G; D011485:Protein Binding; D013696:Temperature
D000581:Amidohydrolases; D010399:Penicillin Amidase; D010400:Penicillin G
Poluchenie i kineticheskie svoĭstva priparata immobilizovannoĭ benzilpenitsillinatsilazy