Mutant enrichment in the colonial alga, Eudorina elegans.
An enrichment procedure has been developed that results in at least a 200 X increase in mutation frequency in the colonial alga, Eudorina elegans. A period of nitrogen starvation followed by treatment with 8-azaguanine results in the death of wild-type cells and the maintenance of mutants. N'-nitro-N-nitro-soguanidine-induced acetate, p-aminobenzoic acid and reduced nitrogen requiring mutants have been isolated by this procedure.
D000085:Acetates / Q000378:metabolism; D062365:Aminobenzoates / Q000378:metabolism; D001375:Azaguanine / Q000494:pharmacology; D056890:Eukaryota / Q000378:metabolism*; D009153:Mutagens; D009154:Mutation; D009584:Nitrogen / Q000172:deficiency / Q000378:metabolism; D009604:Nitrosoguanidines / Q000378:metabolism
D000085:Acetates; D062365:Aminobenzoates; D009153:Mutagens; D009604:Nitrosoguanidines; D009584:Nitrogen; D001375:Azaguanine