On the carbohydrate composition of bovine colostrum trypsin inhibitor.
The trypsin inhibitor from bovine colostrum has been separated into several forms by CM-Sephadex and DEAE-cellulose chromatography. These forms differ in the amount and composition of the carbohydrate they contain, which has been quantitated for four components by gas-liquid chromatography and standrad colorimetric procedures. The monosaccharides fucose, mannose, galactose, galactosamine, glucosamine and sialic acids have been determined. A microheterogeneity was establish ed in the carbohydrate moiety, which amounts to about 40% of the total molecular weight (Mr 11 000 - 14 000) of bovine colostrum inhibitor.
D000818:Animals; D002417:Cattle; D003126:Colostrum / Q000032:analysis*; D005260:Female; D005643:Fucose / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis*; D011247:Pregnancy; D012794:Sialic Acids / Q000032:analysis; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000032:analysis*
D006023:Glycoproteins; D006601:Hexoses; D009005:Monosaccharides; D012794:Sialic Acids; D014361:Trypsin Inhibitors; D005643:Fucose; D005688:Galactosamine; D014357:Trypsin; D005944:Glucosamine