The reaction of choline dehydrogenase with some electron acceptors.
1. The choline dehydrogenase (EC 1.1.99.1) WAS SOLUBILIZED FROM ACETONE-DRIED POWDERS OF RAT LIVER MITOCHONDRIA BY TREATMENT WITH Naja naja venom. 2. The kinetics of the reaction of enzyme with phenazine methosulphate and ubiquinone-2 as electron acceptors were investigated. 3. With both electron acceptors the reaction mechanism appears to involve a free, modified-enzyme intermediate. 4. With some electron acceptors the maximum velocity of the reaction is independent of the nature of the acceptor. With phenazine methosulphate and ubiquinone-2 as acceptors the Km value for choline is also independent of the nature of the acceptor molecule. 5. The mechanism of the Triton X-100-solubilized enzyme is apparently the smae as that for the snake venom solubilized enzyme.
D015086:2,6-Dichloroindophenol; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D004579:Electron Transport; D007700:Kinetics; D008433:Mathematics; D008773:Methylphenazonium Methosulfate; D008930:Mitochondria, Liver / Q000201:enzymology; D010100:Oxygen; D011092:Polyethylene Glycols; D011809:Quinones; D012910:Snake Venoms; D012995:Solubility; D014451:Ubiquinone
D011809:Quinones; D012910:Snake Venoms; D014451:Ubiquinone; D008773:Methylphenazonium Methosulfate; D011092:Polyethylene Glycols; D015086:2,6-Dichloroindophenol; D000429:Alcohol Oxidoreductases; D010100:Oxygen