The separation and identification of dopamine 3-O-sulfate and dopamine 4-O-sulfate in urine of Parkinsonian patients.
A method is described for the isolation and measurement of dopamine 3-O-sulfate and dopamine 4-O-sulfate. The sulfate isomers of dopamine were synthesized chemically by the reaction of dopamine with sulfuric acid. The isomers were isolated by anion-exchange column chromatography and the identities of the two isomers were confirmed by gas chromatography coupled with mass spectrometry. A method is described for the assay of the two isomers by high-pressure liquid chromatography. When Parkinsonian patients were treated with 4.0 g/day of L-dopa, the amount of dopamine 3-O-sulfate excreted in the urine was 19.6 times that of dopamine 4-O-sulfate. When untreated Parkinsonian patients received tracer quantities of 3H-L-dopa either intravenously or orally, 3H-dopamine 3-O-sulfate was the predominant isomer but the amounts were only 3 times those of the 4-isomer. Greater quantities of both isomers were excreted when 3H-L-dopa was given orally as compared to intravenous administration. Since dopamine 3-O-sulfate is the predominant sulfate isomer, it is concluded that sulfate conjugation of dopamine could possibly compete with 3-O-methylation in determining the resultant conjugated product.
D000284:Administration, Oral; D000368:Aged; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004298:Dopamine / Q000138:chemical synthesis / Q000652:urine*; D005260:Female; D006801:Humans; D007263:Infusions, Parenteral; D007536:Isomerism; D007980:Levodopa / Q000008:administration & dosage / Q000627:therapeutic use; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010300:Parkinson Disease / Q000188:drug therapy / Q000652:urine*; D013431:Sulfates / Q000652:urine; D014439:Tyramine / Q000031:analogs & derivatives
D013431:Sulfates; D007980:Levodopa; D004298:Dopamine; D014439:Tyramine