Characteristics of messenger ribonucleoprotein and putative messenger RNA from Ehrlich ascites tumor cells.
Ehrlich ascites tumor cell messenger ribonucleoprotein (mRNP) and putative messenger RNA (mRNA) were isolated under conditions minimizing RNA degradation by ribonucleases and non-specific protein-RNA interaction, mRNP dissociated from polysomes by puromycin and EDTA sedimented to 185S. Putative messenger RNA released by proteinase K sedimented from 50 to 97S, with a maximum molecular weight approximating 6-25 X 10(6) daltons.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D051379:Mice; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm; D012261:Ribonucleoproteins / Q000032:analysis*
D009698:Nucleoproteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012261:Ribonucleoproteins