[The effect of detergent Triton X=100 on the light induced changes in the fluorescence yield of chloroplasts].
It is shown that light induced changes of the fluorescence yield (delta F) of isolated chloroplasts are affected by Triton X-100. delta F value descreases with the increase of the detergent concentration from 0 to 0.03%, increases in the range of 0.03--0.05% and is irreversibly blocked at concentrations more than 0.08--0.1%. The same dependence of delta F on  the detergent concentration is obtained for "digitonin" fragments of chloroplasts enriched in the photosystem 2, but not for fragments enriched in the photosystem 1. Light induced delta F of chloroplasts treated by detergent were activated by hydroxylamine and saturated at lower light intensities than delta F of untreated chloroplasts. Addition of 0.01% Triton resulted in an activation of light induced delta F of chloroplasts with damaged donor part of photosystem 2. It is suggested that the complex  dependence of delta F of chloroplasts on the Triton concentration is due to superposition of several effects: the uncoupling of photophosphorylation, inactivation of the electron transport chain in the donor and acceptor parts of photosystem 2, and changes of acting concentration of Triton X-100 within the range of critical micelle concentration.
D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D008027:Light; D010785:Photophosphorylation / Q000187:drug effects; D010788:Photosynthesis / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D000644:Quaternary Ammonium Compounds; D011092:Polyethylene Glycols
Deĭstvie detergenta Tritona X-100 na fotoindutsirovannye izmeneniia vykhoda fluorestsentsii khloroplastov