Studies on the cultivation of Clostridium oncolyticum M 55. 5th communication: The influence of iron, zinc, and cobaltous ions on the growth and the kininase activity of clostridium oncolyticum M 55 ATCC 13.732.
The precipitation or iron sulfide by hydrogen sulfide excreting Clostridium oncolyticum M 55 led to further examinations in order to avoid this disturbing factor. The method should render the cultivation of Cl. oncolyticum M 55 under definite conditions and should also intensify the formation and the enrichment of the clostridial kininases. Cobaltous ions and zinc granules were found useful for cultivation, because no disturbances increased from both during sterilization and incubation. The determination of the growth cycles and the replication rates showed the usefulness of zinc for substitution of iron. Cobaltous ions did only allow a low replication rate. These results confirmed those showing the influence of chelate-forming agents on the kininase activity. Cobaltous ions were not able to exchange the central atom of the metallo-proteide. The influence of the peptone quality on the kininase activity in presence of zinc was also examined, since examinations with iron media have shown a direct connection between peptone quality and enzyme activity. The results with zinc-containing media were nearly 5-10% lower than those with iron. The results allowed also the conclusion that the kininase excreted by Cl. oncolyticum M 55 is an iron-containing metallo-proteide, whose central atom is exchangeable for zinc. The consequences of the loss of enzyme activity for the oncolytic therapy with Cl. oncolyticum M 55 will be shown in further examinations.
D000085:Acetates; D001920:Bradykinin; D002268:Carboxypeptidases / Q000378:metabolism*; D002712:Chlorides; D003013:Clostridium / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D003035:Cobalt / Q000494:pharmacology*; D003470:Culture Media; D007501:Iron / Q000494:pharmacology*; D001121:Lysine Carboxypeptidase / Q000378:metabolism*; D009573:Nitrites; D015032:Zinc / Q000494:pharmacology*
D000085:Acetates; D002712:Chlorides; D003470:Culture Media; D009573:Nitrites; D003035:Cobalt; D007501:Iron; D002268:Carboxypeptidases; D001121:Lysine Carboxypeptidase; D015032:Zinc; D001920:Bradykinin