Modulation in the rates of incorporation of lipid precursors during the cell cycle.
The rates of incorporation of 3H-choline into phospholipid and of 3H-fucose into glycolipid have been measured during the cell cycle of the murine mastocytoma, P815Y, synchronized by either velocity sedimentation or excess thymidine blockade. The rate of 3H-choline incorporation into acid-insoluble material exhibited two distinct maxima coincident with the early S and G2 periods, whereas the rate of incorporation of 3H-fucose into lipid extractable material was maximal during the G2 period. Variation in rate of incorporation of 3H-choline could not be accounted for by changes in membrane permeability.
D002455:Cell Division; D002460:Cell Line; D002794:Choline / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D005643:Fucose / Q000378:metabolism*; D006017:Glycolipids / Q000096:biosynthesis*; D007700:Kinetics; D010743:Phospholipids / Q000096:biosynthesis*; D013936:Thymidine / Q000378:metabolism
D006017:Glycolipids; D010743:Phospholipids; D005643:Fucose; D004247:DNA; D002794:Choline; D013936:Thymidine