Studies on the intracellular location of enzymes of the photosynthetic carbon-reduction cycle.
Homogenates of dark-pretreated leaves yield two particulate fractions in density gradient centrifugation: one contains chlorophyll (chloroplasts) while a second fraction contains ribulose-1, 5-bisphophate carboxylase, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase and catalase. Addition of a microbody-rich pellet to chloroplasts isolated from dark-pretreated plants largely enhances both oxygen evolution and CO2-fixation into organic compounds. The pathway of CO2 reduction may be part of a membrane system which, under suitable conditions, may separate from the chloroplast as a distinct cytoplasmic entity, having physical properties similar to those of microbodies.
D002245:Carbon Dioxide / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D003624:Darkness; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D008027:Light; D008830:Microbodies / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis; D010944:Plants / Q000378:metabolism*; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism
D002734:Chlorophyll; D002245:Carbon Dioxide; D002374:Catalase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D012273:Ribulose-Bisphosphate Carboxylase; D010100:Oxygen