title: Chromosome markers in Mus musculus: differences in C-banding between the subspecies M.m. musculus and M.m. molossinus.
abstract: Quinacrine (Q-band) and centromeric heterochromatin (C-band) patterns of metaphase chromosomes of two subspecies of Mus musculus were compared. M.m. musculus (the laboratory mouse) and M.m. molossinus (a subspecies from Southeast Asia) had similar Q-band patterns along the length of the chromosomes, but differences were observed in the centromeric region of some chromosomes. The two subspecies had very different distributions of C-band material. Antibodies to 5-methyleytosine were bound to regions of the chromosome corresponding to the C-bands in each animal. These findings support the idea that satellite DNA, which is concentrated in the C-band region, changes more quickly than bulk DNA. The interfertility of these two subspecies permits the development of a musculus strain carrying normal marker chromosomes for genetic studies.
mesh_terms: D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002875:Chromosomes / Q000032:analysis*; D003433:Crosses, Genetic; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004276:DNA, Satellite / Q000032:analysis; D005260:Female; D014644:Genetic Variation; D006570:Heterochromatin / Q000032:analysis*; D007246:Infertility / Q000235:genetics; D008297:Male; D051379:Mice / Q000502:physiology*; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D011796:Quinacrine
chemical_list: D004276:DNA, Satellite; D006570:Heterochromatin; D003596:Cytosine; D011796:Quinacrine