title: [Isolation of chloroplasts and chloroplast DNA from pea leaves].
abstract: A method of isolating DNA from pea chloroplasts (ch-DNA) in CsCl density gradient is described. DNA preparations are free of 5-methylcytosine and have a melting temperature of 86.5 degrees. Denatured DNA molecules completely reassociate for 3 hours at 60 degrees C. It is concluded that the preparations obtained are pure ch-DNA.
mesh_terms: D002499:Centrifugation, Density Gradient / Q000379:methods; D002736:Chloroplasts / Q000032:analysis / Q000166:cytology*; D004247:DNA / Q000302:isolation & purification*; D007700:Kinetics; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D010944:Plants; D013696:Temperature
chemical_list: D004247:DNA
vernacular_title: Vydelenie khloroplastov i khloroplastnoĭ DNK iz list'ev gorokha