Function of 5'-nucleotidase in the uptake of adenosine from AMP by human lymphocytes.
The function of 5'-nucleotidase in nucleoside uptake from AMP was investigated in human lymphocytes by comparing the transport in cells containing this enzyme (5'N+) with that in cells deficient in the activity (5'N-). The rate of adenosine and Pi uptake from AMP was 3.9-fold greater in the 5'N+ then in the 5'N- lymphocytes. There was no difference in transport between these cells when incubated with adenosine or Pi. These results indicate that phosphorylytic cleavage of AMP by 5'-nucleotidase is necessary for the uptake of the nucleotide and Pi moieties by the human lymphocyte.
D000241:Adenosine / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism*; D001693:Biological Transport, Active; D001881:Borates / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D006801:Humans; D007288:Inosine / Q000031:analogs & derivatives / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009708:Nucleotidases / Q000378:metabolism*
D001881:Borates; D003486:Cyanides; D007461:Iodoacetates; D000249:Adenosine Monophosphate; D007288:Inosine; D009708:Nucleotidases; D000241:Adenosine; D005459:Fluorides