[A simple extraction chamber for the analysis of gases by gas chromatography in small blood samples (50 microliters) (author's transl)].
A sample extraction chamber for the determination of O2- CO2- and N2O-concentrations in 50 microliters blood samples by gas chromatography is described. These blood samples can be transferred anaerobically from the ear lobe of a patient or other donor by a doctor or medical auxillary. It is shown that N2O, O2 and CO2 are completely extracted from blood, red cell suspension and HCO3-solutions respectively. When the concentrations of the same sample was repeatedly measured the coefficient of variation was 1.3% for O2 and N2O and 1.2% for CO2.
D001639:Bicarbonates; D001784:Blood Gas Analysis / Q000295:instrumentation*; D001800:Blood Specimen Collection / Q000295:instrumentation; D002245:Carbon Dioxide / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods; D004912:Erythrocytes / Q000032:analysis; D006801:Humans; D008832:Microchemistry / Q000379:methods; D009609:Nitrous Oxide / Q000097:blood; D010100:Oxygen / Q000097:blood
D001639:Bicarbonates; D002245:Carbon Dioxide; D009609:Nitrous Oxide; D010100:Oxygen
Eine einfache Extraktionskammer zur gaschromatographischen Gasanalyse in kleinen Blutproben (50 microliters)