Lipid composition in postnatal methylazoxymethanol-treated swiss albino mouse cerebellum.
Postnatal Swiss albino mice were treated with with methylazoxymethanol acetate (MAM) or saline and sacrificed at 25 days of age. Granule cell depletion resulted. Significant reduction in the cerebellar weight, protein, ganglioside sialic acid , cerebrosides, sulfatides, and phospholipids were documented. There was not, however, selective reduction of any component known to be associated with the synaptic structures. Specific association of cerebellar ganglioside with its granule cell population was not substantiated. Cerebroside/sulfatide ratios were not different in the two groups, indicating that significant alterations previously observed in spinal cord were not present in the cerebellum. It was concluded that the bulk of cerebellar granule cells and their synaptic connections could be deleted without affecting total ganglioside and phospholipid concentrations.
D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D001391:Azo Compounds / Q000494:pharmacology*; D001923:Brain Chemistry / Q000187:drug effects; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D002554:Cerebrosides / Q000032:analysis; D005732:Gangliosides / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008746:Methylazoxymethanol Acetate / Q000494:pharmacology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D013433:Sulfoglycosphingolipids / Q000032:analysis
D001391:Azo Compounds; D002554:Cerebrosides; D005732:Gangliosides; D008055:Lipids; D009419:Nerve Tissue Proteins; D010743:Phospholipids; D013433:Sulfoglycosphingolipids; D008746:Methylazoxymethanol Acetate