[Modification of tRNA 1 Val from yeast with monoperphthalic acid].
A method is proposed for analysis of natural and chemically modified polynucleotides which consists in enzymatic conversion of the polymer or oligomer into nucleosides followed by cation-exchange chromotography on the microcolumns. By using the method developed it was shown that after treatment of the yeast tRNAVal and tRNAPhe with monoperphthalic acid N-oxides of adenosine and cytidine were formed. Poly (U, G) was not modified at a measurable extent whereas GMP was decomposed. In tRNAVal (yeast)the adenosines and cytosines of the anticodon loop and 3'-end are most reactive; it is the case for the C17 of the diHU-loop as well. These data are in agreement with the results obtained for tRNA modification with other reagents and for limited enzymatic hydrolysis of the tRNAVal. The limitations of the reaction of the monoperphthalate with nucleic acids are briefly discussed.
D001483:Base Sequence; D001665:Binding Sites; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000032:analysis; D010649:Phenylalanine; D010795:Phthalic Acids; D012343:RNA, Transfer; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis
D009841:Oligonucleotides; D010795:Phthalic Acids; D012265:Ribonucleotides; D010649:Phenylalanine; D012343:RNA, Transfer; D012260:Ribonucleases
Modifikatsiia drozhzhevoi tRNA 1 Val monoperftalevoĭ kislotoĭ