title: Application of over-run thin-layer and gas-liquid chromatography in the separation of closely related C19O2 steroids.
abstract: An improved method for the separation of epimeric C19O2 steroids and their related allylic alcohols is described. In this method, the steroids are first separated by over-run thin-layer chromatography, and the unresolved groups are further analysed as free or as trimethylsilyl ether derivatives by gas-liquid chromatography. The behaviour of twenty-one C19O2 steroids was investigated by thin-layer chromatography in four systems and by gas-liquid chromatography in four liquid phases. All steroid pairs of similar polarity were resolved by the combination of these two fractionation procedures.
mesh_terms: D015068:17-Ketosteroids / Q000302:isolation & purification; D015113:Androstane-3,17-diol / Q000302:isolation & purification; D000731:Androstanes / Q000302:isolation & purification*; D000734:Androstenediols / Q000302:isolation & purification; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D006914:Hydroxysteroids / Q000302:isolation & purification; D007664:Ketosteroids / Q000302:isolation & purification; D012997:Solvents; D014297:Trimethylsilyl Compounds / Q000302:isolation & purification
chemical_list: D015068:17-Ketosteroids; D000731:Androstanes; D000734:Androstenediols; D006914:Hydroxysteroids; D007664:Ketosteroids; D012997:Solvents; D014297:Trimethylsilyl Compounds; D015113:Androstane-3,17-diol