Rapid purification of 70S RNA from media of cells producing RNA tumor viruses.
Media from cells producing RNA tumor viruses, when treated with sodium dodecyl sulfate and polyvinyl sulfate, yield 70S RNA as the major species binding oligo(dT)-cellulose. The procedure described for purifying 70S RNA requires no special equipment and is suitable for rapidly processing large quantities of media or for purifying RNA from commercially avialable virus, with a 5- to 10-fold higher yield than was obtained using existing methods.
D002460:Cell Line; D002845:Chromatography / Q000379:methods; D003470:Culture Media / Q000032:analysis; D005069:Evaluation Studies as Topic; D009858:Oncogenic Viruses / Q000032:analysis* / Q000254:growth & development; D011145:Polyvinyls; D012328:RNA Viruses / Q000032:analysis* / Q000254:growth & development; D012367:RNA, Viral / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D014779:Virus Replication
D003470:Culture Media; D011145:Polyvinyls; D012367:RNA, Viral; D012967:Sodium Dodecyl Sulfate