Inhibition of the alkaline phosphatase of equine, bovine and rat neutrophilic leukocytes with levamisole at low concentrations.
In this light microscopic study, the inhibitory effect of levamisole on the alkaline phosphatase of equine, bovine and rat neutrophilic leukocytes was examined histochemically, using 0.05, 0.1 and 0.5 mM levamisole in the incubation mixture. Levamisole proved to be a potent inhibitor of neutrophilic alkaline phasphatase already at very low concentrations. Statistically significant differences in the rate of inhibition of the enzyme were found between the species examined. Some observations of the eosinophils were also made; equine eosinophils were found to be considerably resistant towards alkaline phosphatase inhibition with levamisole at the concentrations used.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors* / Q000097:blood; D000818:Animals; D002417:Cattle; D004804:Eosinophils / Q000201:enzymology; D006736:Horses; D007978:Levamisole / Q000494:pharmacology*; D009504:Neutrophils / Q000201:enzymology*; D051381:Rats
D007978:Levamisole; D000469:Alkaline Phosphatase