[Different forms of pyruvate kinase from the rabbit adrenal cortex].
Pyruvatekinase (PK) isoenzymes were isolated by means of (NH4)2SO4 fractionation (20-45% and 50-70% of saturation) and DEAE-cellulose chromatography. Two peaks of PK activity (I and II) were discovered under chromatography of 20-45%  saturation precipitate, and one peak (I-A)-under chromatography of 50-70% saturation precipitate. PK-II in its properties (two-peaks kinetics for phosphoenolpyruvate (PEP), the resistance to effectors) is like L-type PK, which has lost its allosteric properties in the process of long-term isolation at low temperatures. PK-I and PK-I-A have S-form kinetics for PEP. They are inhibited by L-alanine and phenylalanine, and they probably are variants of the initial K(M2) type.
D000302:Adrenal Cortex / Q000201:enzymology*; D000311:Adrenal Glands / Q000201:enzymology*; D000409:Alanine / Q000494:pharmacology; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D007527:Isoenzymes / Q000032:analysis*; D007700:Kinetics; D010649:Phenylalanine / Q000494:pharmacology; D010728:Phosphoenolpyruvate / Q000378:metabolism; D011770:Pyruvate Kinase / Q000032:analysis* / Q000037:antagonists & inhibitors; D011817:Rabbits
D007527:Isoenzymes; D010649:Phenylalanine; D010728:Phosphoenolpyruvate; D011770:Pyruvate Kinase; D000409:Alanine
Razlichnye formy piruvatkinazy iz kory pochki krolika