Studies on chromatin. III. v-Bodies and free DNA in chromatin lacking histone H1.
Chromatin lacking histone H1 was found by electron microscopy to contain 'beaded' deoxyribonucleoprotein fibers. Adjacent beads are connected with each other by threads having a DNA-like appearance. At least some of threads are shown to be free DNA stretches. Average length and the content of free DNA stretches in histone H1-depleted chromatin depends on the ionic conditions of the medium. The appearance of individual beads is similar to that of chromatin subunits or v-bodies [1] in the original chromatin. Thus, in agreement with the X-ray data [2], histone H1 apparently is not required for maintenance of a compact state of DNA in chromatin subunits.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D003852:Deoxyribonucleoproteins / Q000032:analysis; D006657:Histones / Q000032:analysis*; D051379:Mice; D008970:Molecular Weight; D009690:Nucleic Acid Conformation
D002843:Chromatin; D004273:DNA, Neoplasm; D003852:Deoxyribonucleoproteins; D006657:Histones