The allergens of Schistosoma mansoni. II. Further separation by sephadex G-200 and ion-exchange chromatography.
A total of thirty-five antigen fractions were prepared from adult Schistosoma mansoni by extraction into borate-buffered saline, precipitation at pH 4-6, gel-filtration on Sephadex G-100 and G-200, and ion-exchange chromatography on CM- and DEAE-cellulose. The allergic activity of these antigens was assayed by a modified Prausnitz-Küstner (P-K) type reaction in rats. The results showed that the allergen-reagin axis in rat schistosomiasis is a multicomponent system involving molecules of widely different chemical nature and molecular weight, rather than a simple, single antigen-antibody interaction. The significance of these findings to the use of purified antigens for the diagnosis of schistosomiasis in the field is discussed.
D000485:Allergens / Q000032:analysis; D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006969:Hypersensitivity, Immediate; D008970:Molecular Weight; D051381:Rats; D011935:Reagins; D012550:Schistosoma mansoni / Q000276:immunology*
D000485:Allergens; D000941:Antigens; D011935:Reagins