title: A technique for in situ karyotyping of primary amniotic fluid cell cultures.
abstract: A time proven technique is described for growing amniotic fluid cell cultures on cover glasses in Leighton tubes and for processing the mitotic cells in situ. Karyotyping the clones in situ eliminates most of the problems caused by somatic chromosome mutations in vitro and by maternal cell growth.
mesh_terms: D000649:Amniocentesis; D002478:Cells, Cultured; D002999:Clone Cells; D003470:Culture Media; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008297:Male; D009154:Mutation; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods; D012732:Sex Determination Analysis
chemical_list: D003470:Culture Media