Some new bifunctional reagents for the conjugation of a protein with an amino compound in water.
Five symmetrical bifunctional reagents (I-V) have been synthesized and tested for their usefulness for the conjugation of albumin with tryptophan, in aqueous solution. With I and II about 17, with IV and V about 8 tryptophan residues could be introduced in the native protein. The difference is discussed. Reagent III has a very low solubility in water but may be useful if organic solvents are permissible.
D001665:Binding Sites; D006801:Humans; D007202:Indicators and Reagents; D011485:Protein Binding; D011487:Protein Conformation; D011726:Pyridinium Compounds; D012709:Serum Albumin; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013388:Succinimides; D013450:Sulfones; D014364:Tryptophan / Q000031:analogs & derivatives
D007202:Indicators and Reagents; D011726:Pyridinium Compounds; D012709:Serum Albumin; D013388:Succinimides; D013450:Sulfones; D014364:Tryptophan