[Standardized system for incorporating labelled amino acid in vitro with rat spleen fractions].
The authors describe a standardized system devoid of cells with polyribosomes and total cellular juice, obtained from a rat spleen. They discuss the dependence between incorporating capability and the temperature of incubation, concentration of magnesium ions as well as the significance of single components for the optimal capacity of the incorporating system.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism; D008722:Methods; D010649:Phenylalanine / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D011849:Radioactive Tracers; D011868:Radioisotopes; D051381:Rats; D013154:Spleen / Q000378:metabolism*
D000596:Amino Acids; D002250:Carbon Radioisotopes; D011849:Radioactive Tracers; D011868:Radioisotopes; D010649:Phenylalanine
Standartizirana sistema za vkliuchvane na beliazana aminokiselina in vitro s fraktsii ot slezka na plukh