title: [Kinetic studies of leucyl-tRNA synthetase: measurement of the ratio rates of overall and exchange reaction].
abstract: Equations for the ratio of the rates of aminoacylation of tRNA and the rates of ATP-PP exchange show characteristic dependences on the concentrations of substrates, products and inhibitors according to the reaction mechanism. Analyses of leucyl-tRNA synthesis show that these differences may be explained by the ratios of rates for the individual partial reactions. The ratio of rates as a function of tRNA concentration suggests that alternative mechanisms might exist but present methods do not allow the distinction of these possibilities.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D001665:Binding Sites; D007935:Leucine-tRNA Ligase / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D000604:Amino Acyl-tRNA Synthetases; D007935:Leucine-tRNA Ligase
vernacular_title: Kineticheskoe issledovanie leĭtsil-tRNK-sintetazy. Opredelenie otnositel'nykh skorostĭ summarnogo protsessa i stadii obmena pirofosfata