[Studies on the metabolism of benzo(a)pyrene in alveolar-macrophages. II. Kinetics of metabolism and characterization of the metabolites (author's transl)].
The purpose of this communication is to study the metabolism of benzo(a)pyrene by alveolar macrophages from guinea pigs. A column chromatographic technique using a gradient elution (fig. 1) followed by thin layer chromatography has been employed to separate the metabolites. Alveolar macrophages in culture produce metabolites (fig. 2), which remain predominantly in the cells (tab. 1). The presence of serum in the medium modifies the kind of metabolites formed (fig. 3,4). In order to identify the metabolites, the products formed by liver microsomes from benzo(a)pyrene have been separated (fig. 5), and spectroscopically characterised. Unknown substances have been detected by thin layer chromatography (fig. 6,8). The spectra of 3-OH-benzo(a)pyrene 4,5-dihydro-4,5-dihydroxybenzo(a)pyrene, 7,8-dihydro-7,8-dihydroxybenzo(a)pyrene, 9,10-dihydro-9, 10-dihydroxybenzo(a)pyrene could be identified (fig. 7, 9, 10, 11). The characterisation of labelled metabolites formed by alveolar macrophages in culture has been achieved by comparison of their chromatographic behaviour with that of the reference metabolites obtained from liver microsomes (fig. 12). With the exception of the 4,5-diol all metabolites were present in extracts from alveolar macrophages. The results are discussed and compared with published data (tab. 2). The present knowledge about the pathways of metabolism of benzo(a)pyrene is summarized in a scheme (fig. 13).
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism*; D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media; D006168:Guinea Pigs; D008264:Macrophages / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology
D001580:Benzopyrenes; D003470:Culture Media
Untersuchungen über den Metabolismus von Benzo(a)pyren in Alveolarmakrophagen II. Kinetik des Abbaus und Charakterisierung der Metabolite