Effect of some inhibitors on kanamycin formation by Streptomyces kanamyceticus.
The formation of kanamycin is markedly inhibited by mercuric chloride, sodium iodoacetate, 2,4-dinitrophenol, sodium arsenite and sodium azide particularly when these are added at the start of fermentation. Less inhibition of kanamycin synthesis is observed in case of sodium 5,5-diethylbarbiturate, malonic acid, sodium arsenate and sodium fluoride. Inhibition of kanamycin synthesis is associated with growth inhibition in case of 2,4-dinitrophenol, sodium arsenite and sodium azide. Bacitracin and D-cycloserine have a stimulatory effect on kanamycin synthesis with slight inhibition of cellular growth. This stimulation might be due to accumulation of cell wallintermediates--aminosugar and sugar--which are shunted to the pathway of kanamycin synthesis. Penicillin lowers kanamycin synthesis by 65 percent as compared with 19 percent reduction of cellular growth. Chloramphenicol has a stimulatory effect at lower concentration (20 mug/ml), when it is added at 24 h of fermentation. At higher concentration (50 mug/ml) chloramphenicol shows marked inhibition of both cellular growth and antibiotic biosynthesis.
D001151:Arsenic / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D001414:Bacitracin / Q000494:pharmacology; D001463:Barbiturates / Q000494:pharmacology; D002473:Cell Wall; D002701:Chloramphenicol / Q000494:pharmacology; D003523:Cycloserine / Q000494:pharmacology; D003864:Depression, Chemical; D004140:Dinitrophenols / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology; D007612:Kanamycin / Q000096:biosynthesis*; D008314:Malonates / Q000494:pharmacology; D008628:Mercury / Q000494:pharmacology; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism*
D001386:Azides; D001463:Barbiturates; D004140:Dinitrophenols; D007461:Iodoacetates; D008314:Malonates; D001414:Bacitracin; D007612:Kanamycin; D002701:Chloramphenicol; D003523:Cycloserine; D008628:Mercury; D001151:Arsenic; D005459:Fluorides