[Use of peroxidase markers in the research and diagnosis of paludism].
The indirect peroxidase labelled technic for the diagnosis and research in malaria rests on the same principle than immunofluorescence test (I. F.). Homologous or heterologous antigens are incubated with patient serum in the same conditions than for I. F., and then with peroxydase, labelled, conjugate diluted 1/200. The resulting complex is colored by the diaminobenzidine method of Graham and Karnovski. The coloration is observed in light microscopy. This technic as sensitive as immunofluorescence test can be done, with a cheaper microscopic material. The possibility of studying the conjugate fixation in electron microscopy opens new direction for research.
D000818:Animals; D000936:Antigen-Antibody Complex; D006041:Goats / Q000276:immunology; D008288:Malaria / Q000175:diagnosis* / Q000201:enzymology / Q000276:immunology; D010544:Peroxidases; D012756:Sheep / Q000276:immunology
D000936:Antigen-Antibody Complex; D010544:Peroxidases
Utilisation de marqueurs à la peroxydase pour la recherche et le diagnostic immunologique du paludisme