Quantitative autoradiographic assessment of 3H-estradiol uptake in immunocytochemically characterized pituitary cells.
Dry-mount autoradiography was combined with peroxidase immunocytochemistry to examine estrogen uptake in four pituitary cell types. Quantification by silver grain counts was used to compare 3H-estradiol uptake in nuclei of pituitary cells 60 min after i.v. injection into short-term (control) and long-term ovariectomized and in long-term thyroidectomized rats. Under all three hormonal states, the order of labeling intensity was: gonadotropes greater than somatotropes greater than lactotrops greater than thyrotropes. Long-term ovariectomy caused a significant increase in estrogen uptake of gonadotropes, somatotropes and lactotropes, while uptake in thyrotropes decreased. Long-term thyroidectomy decreased uptake in somatotropes, lactotropes and thyrotropes while gonadotropes remained unchanged.
D000818:Animals; D001345:Autoradiography; D002369:Castration; D004958:Estradiol / Q000378:metabolism*; D004967:Estrogens / Q000502:physiology; D005246:Feedback; D005260:Female; D006065:Gonadotropins, Pituitary / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism; D006651:Histocytochemistry; D006735:Horseradish Peroxidase / Q000276:immunology; D010902:Pituitary Gland / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism; D051381:Rats; D013965:Thyroidectomy; D013972:Thyrotropin / Q000032:analysis
D004967:Estrogens; D006065:Gonadotropins, Pituitary; D004958:Estradiol; D011388:Prolactin; D013972:Thyrotropin; D013006:Growth Hormone; D006735:Horseradish Peroxidase