Aminopeptidase B in human serum.
The activity of arginine aminopeptidases in human serum was studied with special reference to the possible involvement of enzymes resembling aminopeptidase B (APB). The serum samples were obtained from 10 patients with various acute inflammatory processes, both during the acute phase and after treatment with penicillin. The administration of penicillin to the patients did not result in noticable changes in the chromatographic pattern of the APB-like enzyme. The disappearance of the acute symptoms did not significantly affect the overall specific activity of arginine aminopeptidases in serum. The results showed that human serum does contain, contrary to earlier reports, an enzyme corresponding to APB, even though in very low concentrations. The low enzyme activity was revealed by prolonged reaction times and by the use of larger samples in chromatography than earlier. The APB-like enzyme in serum may have derived from blood cells.
D000038:Abscess / Q000188:drug therapy / Q000201:enzymology*; D000208:Acute Disease; D000626:Aminopeptidases / Q000097:blood*; D004938:Esophageal Neoplasms / Q000201:enzymology; D006801:Humans; D007249:Inflammation / Q000201:enzymology; D007700:Kinetics; D009059:Mouth Diseases / Q000188:drug therapy / Q000201:enzymology*; D010406:Penicillins / Q000627:therapeutic use; D013329:Structure-Activity Relationship
D010406:Penicillins; D000626:Aminopeptidases