High-pressure liquid chromatographic determination of 2-aminoethyl hydrogen sulfate in plasma and urine.
A sensitive and specific method for the analysis of 2-aminoethyl hydrogen sulfate in biological fluids by high-pressure liquid chromatography is described. Aliquots from deproteinized plasma or from acidified urine were subjected to ion-exchange clean-up by eluting with water through two columns packed with Dowex 1-X4 (Cl- form) and Dowex 50W-X8 (H+ form) resins. Recoveries in the eluates were consistent and quantitative (95-100%). Effluents from such treatment were chromatographed on a Durrum cation-exchange column with 0.05 M citrate buffer containing 0.15 N Na+ (pH 2.6). Detection was accomplished with an automated fluorescamine detection system. Using 2 ml of plasma or urine, 2-aminoethyl hydrogen sulfate could be assayed at concentrations of 0.3 mug/ml. Linear responses were observed up to at least 133 mug/ml. The method is both accurate and reproducible with a relative standard deviation of about +/- 4.0%.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D004232:Diuretics; D004285:Dogs; D004983:Ethanolamines; D005021:Ethylamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D008832:Microchemistry; D013431:Sulfates / Q000032:analysis / Q000097:blood / Q000652:urine
D004232:Diuretics; D004983:Ethanolamines; D005021:Ethylamines; D013431:Sulfates; C020619:ethanolamine O-sulfate