[Methods of determining admixtures in commercial preparations of tetracycline].
The results and conditions of separation and quantitative determination of the admixtures of epi- and anhydro-derivatives of tetracycline in its market products were compared and analyzed using the following chromatographic methods: chromatography in a thin layer of a sorbent in 2 modifications, circular paper chromatography, chromatography on a column of gel Sephadex G-25. The comparison of the results of the study provided recommendation of the column chromatography with gel Sephadex G-25 for separate or summation quantitative determination of 4-epitetracycline. The method of chromatography in a thin layer of a sorbent may be successfully used for semiquantitative determination of the admixture levels in tetracycline market drugs.
D002850:Chromatography, Gel / Q000379:methods; D002854:Chromatography, Paper / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003720:Densitometry; D004340:Drug Contamination; D013752:Tetracycline / Q000032:analysis*; D013754:Tetracyclines / Q000032:analysis
D013754:Tetracyclines; D013752:Tetracycline
Metody opredeleniia primeseĭ v tovarnykh preparatakh tetratsiklina