Adaptation of BHK-21 cells to growth in shaker culture and subsequent challenge by Japanese encephalitis virus.
Baby hamster kidney (BHK-21) cells were adapted to grow in shaker culture using Waymouth medium 752/1 containing 20 mM N-2-hydroxyethyl-piperazine-N'-2'-ethanesulfonic acid buffer and supplemented with 2.5% (vol/vol) calf serum, 0.002% (wt/vol) sodium oleate, and 0.2% fatty acid-free bovine serum albumin (WO2.5). Infectivity of Japanese encephalitis virus grown in the cells adapted to WO2.5 approached 2 x 10(8) plaque-forming units per ml. The culture volume of infected cells was reduced fivefold 12 h after infection. This step resulted in a 10-fold increase in infectivity over that obtained from infected cultures not subjected to volume reduction.
D000818:Animals; D001798:Blood Proteins; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D003470:Culture Media; D004664:Encephalitis Virus, Japanese / Q000254:growth & development*; D006531:HEPES; D007668:Kidney; D009829:Oleic Acids; D012710:Serum Albumin, Bovine; D014779:Virus Replication
D001798:Blood Proteins; D003470:Culture Media; D009829:Oleic Acids; D012710:Serum Albumin, Bovine; D006531:HEPES