Delayed hypersensitivity to mono-azobenzene-arsonate-N-acetyl-L-tyrosine. I. Induction of cellular sensitivity in the rat.
In the guinea pig, mono-ABA-Tyrosone (ABA-Tyr) has been extensively studied as a hapten capable of inducing delayed type hypersensitivity in the absence of circulating antibodies. This model has been extented to the rat, and cellular sensitivity has been assessed both "in vivo" (skin reaction) and "in vitro" (cellular proliferation and lymphocyte migration inhibition). All the reactions studied follow a reproducible pattern with maximal positivity between 4 and 13 days, and negative responses after 20 days. Circulating antibodies were not detectable at any period after immunization. In view of the original properties of ABA-Tyr, and of the advantage of the rat as an experimental animal, the proposed model can be expected to facilitate further studies on the cellular mechanisms of delayed hypersensitivity.
D000818:Animals; D001391:Azo Compounds / Q000276:immunology*; D002464:Cell Migration Inhibition; D005620:Freund's Adjuvant; D006386:Hemagglutination Tests; D006968:Hypersensitivity, Delayed; D008198:Lymph Nodes / Q000187:drug effects / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012882:Skin Tests; D013936:Thymidine / Q000378:metabolism; D014443:Tyrosine / Q000276:immunology*; D010132:p-Azobenzenearsonate / Q000276:immunology*
D001391:Azo Compounds; D014443:Tyrosine; D010132:p-Azobenzenearsonate; D005620:Freund's Adjuvant; D013936:Thymidine