Modification of the surface structure of murine peritoneal macrophages following chemotherapy. A scanning electron microscopic study.
The effects on peritoneal macrophages of some chemotherapeutic agents administered subcutaneously to conventional male AB/Jena mice were studied. beta-[1-ethyl-(5-bis-(beta-chloroethyl)-aminobenzimidazolyl-(2)]-DL-alanine (IMET 2925), beta-[1-phenyl-5-bis-(beta-chloroethyl)-amino-benzimidazolyl-(2)]-DL-alanine (IMET 3164), and gamma-[1-phenyl-5-bis-(beta-chloroethyl)-amino-benzimidazolyl-(2)]-butyric acid (IMET 37/70) evoked a pronounced variation of the cell surface consisting in a shortening, coarsening and reduction in number of the microvili and other projections. Likewise, 1.3-bis(piperidinomethyl)-5-ethyl-5-phenyl-barbituric acid induced a flattening of the surface, but the structures appeared to be increased in number per area. Chlorambucil, 6-mercaptopurine, and 5-fluoro-uracil failed to provoke any obvious change in the threedimensional cell surface structure. These morphological findings are consistent with functional results reported previously.
D000818:Animals; D001463:Barbiturates / Q000494:pharmacology; D001562:Benzimidazoles / Q000494:pharmacology; D002699:Chlorambucil / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D015122:Mercaptopurine / Q000494:pharmacology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010537:Peritoneum / Q000648:ultrastructure*
D001463:Barbiturates; D001562:Benzimidazoles; D002699:Chlorambucil; D015122:Mercaptopurine