The binding of 8-anilinonaphthalene-1-sulphonate to cytoplasmic aspartate aminotransferase from pig heart.
Anilinonaphthalenesulphonate binds to cytoplasmic aspartate aminotransferase with high affinity (Kd about 10 muM) and with a stoicheiometry of one molecule per dimer. It is not displaced by aliphatic or aromatic dicarboxylate substrate analogues. The enzyme is believed to be a symmetrical dimer with identical subunits; it can evidently function asymmetrically in binding anilinonaphthalenesulphonate.
D000817:Anilino Naphthalenesulfonates / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D001665:Binding Sites; D003593:Cytoplasm / Q000201:enzymology; D007700:Kinetics; D008968:Molecular Conformation; D009206:Myocardium / Q000201:enzymology*; D013552:Swine
D000817:Anilino Naphthalenesulfonates; D001219:Aspartate Aminotransferases