title: Skim passage: a rapid method of adapting influenza viruses to new host tissues.
abstract: Skim passage is a series of single-growth cycles whose earliest yield is separated and used, without dilution, for further passages. The method allows effective selection of fast-growing mutants, and the time of adaptation can be shortened to a few days instead of the several weeks or months taken by the conventional sequence of blind passages. The technical details are illustrated by three examples, which also serve to display the scope and limitations of the method.
mesh_terms: D000222:Adaptation, Physiological; D000482:Allantois; D000818:Animals; D002417:Cattle; D002460:Cell Line; D046508:Culture Techniques; D006389:Hemagglutinins, Viral; D007668:Kidney; D051379:Mice; D009154:Mutation; D009975:Orthomyxoviridae / Q000254:growth & development* / Q000276:immunology; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D006389:Hemagglutinins, Viral