Characterization of pulmonary surfactant from ox, rabbit, rat and sheep.
1. Pulmonary surfactants from ox, rabbit, rat and sheep were isolated and analysed. 2. All preparations had a high anenoic phosphatidylcholine content and would produce stable surface tensions of 0.01 Nm-1 or less. 3. Protein content was 8-18% of the dry weights. A number of proteins were observed; their overall composition were high in hydrophobic amino acid residues. 4. Lipid content varied from 79% (ox) to 90% (rabbit) with phosphatidylcholine representing from 58% (sheep) to 83% (rabbit) of the total lipid. The surfactant preparations were rather similar in lipid composition except that sheep surfactant contained about 10% lysophosphatidylcholine. 5. Hexadecanoic acid was the principal fatty acid. It was particularly high in phosphatidylcholine. 6. Phosphatidylglycerol was a minor constituent of all surfactants but phosphatidyldimethylethanolamine was not detected.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011663:Pulmonary Surfactants / Q000032:analysis / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013500:Surface Tension
D000596:Amino Acids; D005227:Fatty Acids; D008055:Lipids; D011506:Proteins; D011663:Pulmonary Surfactants