Parallel electrophoretic fractionation of alkaline phosphatase and serum protein on cellulose acetate strips: clinical evaluation.
We describe here a simple, time-saving method for separating alkaline phosphatases on cellulose acetate, with a parallel detection of serum protein pattern. The staining procedure is based on a coupling reaction between the reaction product of alpha-naphthol with Fast Red Violet LB. The enzymatic reaction is done by bringing the cellulose acetate strip containing the phosphatase band into contact with the surface of a solid agar/substrate mixture. The whole procedure requires 80 min. The method has some features in common with other recently proposed procedures but is more reproducible and rapid because of the choice of the reagents and an improved yield of the coupling step.
D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood*; D001797:Blood Protein Electrophoresis / Q000379:methods*; D001847:Bone Diseases / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D005069:Evaluation Studies as Topic; D005260:Female; D006505:Hepatitis / Q000097:blood; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D007565:Jaundice / Q000097:blood; D008103:Liver Cirrhosis / Q000097:blood; D008297:Male; D009369:Neoplasms / Q000097:blood; D011247:Pregnancy
D007527:Isoenzymes; D000469:Alkaline Phosphatase