Detection of carcinoembryonic antigen in tissue sections by immunoperoxidase.
A triple-bridge, indirect, immunoperoxidase method for detecting and localizing carcinoembryonic antigen (CEA) in tissue sections is described. By this technique, a cell-surface localization of CEA in colonic carcinoma and ovarian mucinous cystadenocarcinoma cells could be visualized. In the case of the colonic cancer, both the tumor from the descending colon and a metastasis to the skin gave positive peroxidase reactions for CEA. This immunocytochemical method for demonstrating the presence of CEA functioned in both frozen, ethanol-fixed and formalin-fixed, paraffin-embedded tissues, thus making it applicable for use with tissue sections conventionally prepared for light microscopy.
D000230:Adenocarcinoma / Q000276:immunology; D000818:Animals; D002272:Carcinoembryonic Antigen / Q000008:administration & dosage / Q000032:analysis*; D003110:Colonic Neoplasms / Q000276:immunology; D003537:Cystadenoma / Q000276:immunology; D005260:Female; D006041:Goats; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G; D007279:Injections, Subcutaneous; D010051:Ovarian Neoplasms / Q000276:immunology; D011817:Rabbits
D002272:Carcinoembryonic Antigen; D007106:Immune Sera; D007074:Immunoglobulin G; D006735:Horseradish Peroxidase