Delayed hypersensitivity to mono-azobenzene arsonate-N-acetyl-L-tyrosine. II. An operational model in the mouse.
Immunization of the mouse by free mono-ABA-Tyrosine (ABA-Tyr) in complete Freund's adjuvant leads to a specific sensitization. This can be assessed by increase in ear thickness after challenge with ABA-Protein conjugates. The intensity of the skin reaction is directly related to both the antigen dosage and the amount of BCG incorporated in the adjuvant. A close correlation has been shown between delayed skin reaction and "in vitro" lymphocyte proliferation in the presence of either ABA-Protein or free ABA-Tyr. In both "in vivo" and "in vitro" tests, sensitization is detectable from day 5 with a maximum on day 7. Contrasting with this strong cellular response, no antibody can be found. Thus, mouse sensitization with ABA-Tyr provides an operational model for pure cellular immunity. The extension of ABA-Tyr model to mouse may vacilitate the analysis of the involved cell populations.
D000818:Animals; D001391:Azo Compounds / Q000276:immunology* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005620:Freund's Adjuvant; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012882:Skin Tests; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000276:immunology / Q000494:pharmacology
D001391:Azo Compounds; D014443:Tyrosine; D005620:Freund's Adjuvant; D013936:Thymidine