Separation and characterization of the reduced metabolites of the 18-hydroxydeoxycorticosterone hormone by gas-liquid chromatography-mass spectrometry. Occurrence of stereoisomeric forms in rat adrenals and liver.
In order to study the metabolism of 18-OH-11-deoxycorticosterone, the mineralocorticoid hormone responsible for hypertension in rats and humans, we have synthesized the following dihydrogenated and tetrahydrogenated reference derivatives: 18,21-dihydroxy-5alpha-pregnane-3,20-dione, 18,21-dihydroxy-5beta-pregnane-3,20-dione, 3alpha,18,21-trihydroxy-5alpha-pregnan-20-one (I), 3beta,18,21-trihydroxy-5alpha-pregnan-20-one (II), 3alpha,18,21-trihydroxy-5beta-pregnan-20-one (III) and 3beta,18,21-trihydroxy-5beta-pregnan-20-one (IV). A complete separation of these compounds from each other and from tetrahydrocorticosterone isomers has been realized only by the association of thin-layer chromatography and gas-liquid chromatography on high-efficiency glass capillary columns. Characterization by gas-liquid chromatography-mas spectrometry is described. The stereoisomer distribution in rats is: adrenals of adult males and females (compounds I and II in the ratio 4:1); adrenals of 23-day-old males and females (compounds I, II and III, 11:11:3); liver of females (compound I and traces of III); and the liver of adult male (compounds II, III and IV, 4.5:4.5:1).
D015070:18-Hydroxydesoxycorticosterone / Q000032:analysis / Q000378:metabolism; D000311:Adrenal Glands / Q000032:analysis; D000328:Adult; D000818:Animals; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003900:Desoxycorticosterone / Q000031:analogs & derivatives*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006973:Hypertension / Q000378:metabolism; D007536:Isomerism; D008099:Liver / Q000032:analysis; D008297:Male; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D051381:Rats; D013237:Stereoisomerism
D015070:18-Hydroxydesoxycorticosterone; D003900:Desoxycorticosterone