Purification of nuclear ribonucleoprotein complexes containing poly(adenylic acid).
Nuclear 14 S ribonucleoprotein particles containing poly(adenylic acid) were isolated from Ehrlich ascites carcinoma cells and purified by a two step method. The nuclear extract obtained at an ionic strength of 0.1 was treated by pancreatic and T1 ribonucleases and sedimented in a 15-30% sucrose gradient. The 14 S peak containing 50-60% poly(adenylic acid) was chromatographed on a poly(dT)-Sepharose column. The particles thus obtained were of high purity: they had an adenosine content of 90% and a protein moiety characteristic of the 14 S nuclear particles i.e. polypeptides with molecular weight of 51 000, 61 000 and 69 000 daltons.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis; D009698:Nucleoproteins / Q000302:isolation & purification*; D010179:Pancreas / Q000201:enzymology; D010455:Peptides / Q000032:analysis; D011061:Poly A / Q000032:analysis*; D012260:Ribonucleases; D012261:Ribonucleoproteins / Q000302:isolation & purification*; D012265:Ribonucleotides / Q000032:analysis
D009363:Neoplasm Proteins; D009698:Nucleoproteins; D010455:Peptides; D012261:Ribonucleoproteins; D012265:Ribonucleotides; D011061:Poly A; D012260:Ribonucleases