title: [Characteristics of hybrid strains of actinomycetes, prepared by crossing Streptomyces coelicolor A3(2) and Streptomyces lividans 66 strains].
abstract: Pecularities of crosses between strains of Streptomyces coelicolor A3(2) and S. lividans 66 are described. Recombinants which contain different contributions from parental chromosomes are shown to arise in these crosses. Due to a low frequency of recombination which seems to be the result of incomplete homology between A3(2) and 66 chromosomes, heteroclone and heterokaryone colonies are formed. These may be considered to be intermediate stages in the process of recombinant formation. Results of crosses between A3(2) derivatives of various fertility types are analysed in view of a suggestion that there exist two modes of genetic material transfer in Actinomycetes: by means of conjugation and via heterokaryone formation stage. The study of recombinant properties reveals that in the presence of sex plasmid SCPI F two substances are formed, one of which (IAM) inhibits airial mycelium and the other (ISM)--substrate mycelium growth. In addition, not only SCPI presence but the involvement of certain A3(2) genes seems to be required for ISM synthesis. A part of recombinants is shown to possess some new antibiotic properties differing from those of original parental strains namely extension of their antimicrobic spectrum for Actinomycetes and bacteria.
mesh_terms: D003433:Crosses, Genetic; D006824:Hybridization, Genetic; D011995:Recombination, Genetic; D013302:Streptomyces
vernacular_title: Kharakteristika gibridnykh shtammov aktinomitsetov, poluchennykh v skreshchivanii mezhdu shtammami Streptomyces coelicolor A3(2) i Streptomyces lividans 66