title: Artifacts in the radioimmunoassay of peptide hormones in gastric and duodenal secretions.
abstract: Observations of apparently high concentrations of gastrin and insulin in gastric and duodenal secretions as determined by radioimmunoassay may arise from artifacts caused by proteolytic enzymes. The double-antibody and charcoal separation techniques as commonly employed fail to recognize extensive proteolytic damage to labeled antigen and to antibody.
mesh_terms: D000937:Antigen-Antibody Reactions; D004386:Duodenum; D005750:Gastric Juice / Q000032:analysis* / Q000201:enzymology; D005755:Gastrins / Q000032:analysis; D006801:Humans; D007328:Insulin / Q000032:analysis; D007419:Intestinal Secretions / Q000032:analysis* / Q000201:enzymology; D010447:Peptide Hydrolases / Q000502:physiology; D011863:Radioimmunoassay
chemical_list: D005755:Gastrins; D007328:Insulin; D010447:Peptide Hydrolases