Fluorometry of plasma amino nitrogen, with use of fluorescamine.
We describe a fluorometric procedure, with use of fluorescamine, for determining plasma amino nitrogen in a 10-mul sample of deproteinized plasma. Values for apparently healthy human subjects agree well with those obtained by spectrophotometry. The simplicity and speed of the assay recommends it as a screening procedure for detecting aminoacidopathies.
D000328:Adult; D000592:Amino Acid Metabolism, Inborn Errors / Q000097:blood / Q000175:diagnosis*; D000596:Amino Acids / Q000097:blood*; D001798:Blood Proteins; D005069:Evaluation Studies as Topic; D005260:Female; D005450:Fluorescamine; D005470:Fluorometry; D006801:Humans; D008297:Male; D008403:Mass Screening; D009584:Nitrogen / Q000097:blood*; D013141:Spiro Compounds
D000596:Amino Acids; D001798:Blood Proteins; D013141:Spiro Compounds; D005450:Fluorescamine; D009584:Nitrogen