title: Segregation of histone fractions from purified rat pancreas nuclei by isoelectric focusing.
abstract: The present report describes the purification of nuclei from rat pancreas and the use of isoelectric focusing for the segregation of histone fractions, a heterogeneous mixture with respect to charge. This procedure employs gels of 5% polyacrylamide as support matrices, in 6.25 M urea, and spans a pH range of 7.5-10.8. It was, therefore, found adequate for study of the microheterogeneity of histones. The banding pattern of histone fractions in pancreas development using electrofocusing is presented.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D010179:Pancreas / Q000032:analysis* / Q000254:growth & development / Q000648:ultrastructure; D051381:Rats
chemical_list: D006657:Histones; D000255:Adenosine Triphosphate