Segregation of histone fractions from purified rat pancreas nuclei by isoelectric focusing.
The present report describes the purification of nuclei from rat pancreas and the use of isoelectric focusing for the segregation of histone fractions, a heterogeneous mixture with respect to charge. This procedure employs gels of 5% polyacrylamide as support matrices, in 6.25 M urea, and spans a pH range of 7.5-10.8. It was, therefore, found adequate for study of the microheterogeneity of histones. The banding pattern of histone fractions in pancreas development using electrofocusing is presented.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D010179:Pancreas / Q000032:analysis* / Q000254:growth & development / Q000648:ultrastructure; D051381:Rats
D006657:Histones; D000255:Adenosine Triphosphate