Substances interfering with the gas-liquid chromatographic determination of T-2 mycotoxin.
Two substances interfering with the gas-liquid chromatographic (GLC) detection of T-2 mycotoxin were identified as 1-glyceryl-monooleate and 1-glycerylmonolinoleate. These monoglycerides are natural products formed by species of Fusarium growing on cereal grains and are also additives contained in liquid vegetable and animal fats added to the feed mixture. The monoglycerides can be removed from the analytical sample by resolution by thin layer chromatography prior to separation by GLC. Trimethylsilyl ether derivatives of the monoglycerides and T-2 toxin have almost identical retention times on 3% OV-1 columns, whereas the trifluoroacetyl and pentafluoropropionyl derivatives give baseline separation on the same column. The monoglycerides can be misidentified as the T-2 toxin in analyses involbing GLC.
D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004340:Drug Contamination; D002523:Edible Grain / Q000032:analysis; D005463:Fluoroacetates; D005989:Glycerides; D008738:Methyl Ethers; D009183:Mycotoxins / Q000032:analysis*; D012717:Sesquiterpenes / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis*
D005463:Fluoroacetates; D005989:Glycerides; D008738:Methyl Ethers; D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes