Nature of progesterone action on amino acid uptake by isolated full-grown oocyte of Xenopus laevis.
L-leucine uptake into full-grown oocytes of Xenopus laevis is a saturable process which is Na+ dependent and presumably coupled to Na+ gradient. Our results indicate that progesterone (10(-6) M) Blocks abruptly, around the germinal vesicle breakdown, the saturable transport of L-leucine. p-Chloromercuribenzoate (10(-4) M) induces maturation and after a short lag of time strongly inhibits L-leucine uptake. Cycloheximide prevents progesterone-induced maturation and permeability changes.
D000818:Animals; D001693:Biological Transport, Active; D002729:Chloromercuribenzoates / Q000494:pharmacology; D005260:Female; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology; D014981:Xenopus
D002729:Chloromercuribenzoates; D011374:Progesterone; D012964:Sodium; D007930:Leucine