Photometric determination of nitrogen. Wet incineration followed by formation of indophenol blue with salicylate/hypochlorite.
Ammonia in wet incineration residues has been analyzed photometrically by a modified Berthelot reaction using a water-free digestion mixture. Phenol is substituted by salicylate in the dye-producing reaction. The method can be adapted for any biological material with a minimum content of 0.04 mug N/sample. Nonprotein nitrogen may be determined in the supernatant of TCA precipitates.
D006801:Humans; D007202:Indicators and Reagents; D009584:Nitrogen / Q000032:analysis* / Q000097:blood / Q000652:urine; D013053:Spectrophotometry / Q000379:methods
D007202:Indicators and Reagents; D009584:Nitrogen