Purification and properties of rat brain pyruvate carboxylase.
Rat brain pyruvate carboxylase was purified 2000-fold and some of its properties and kinetic parameters were investigated. The use of (NH4)2SO4 gradient solubilization on a Celite column and precipitation with polyethylene glycol permitted purification to an estimated 20% purity. Except for a few subtle kinetic differences this enzyme is indistinguishable from rat liver pyruvate carboxylase.
D000645:Ammonium Sulfate; D000818:Animals; D001360:Avidin / Q000494:pharmacology; D001921:Brain / Q000201:enzymology*; D011232:Chemical Precipitation; D004789:Enzyme Activation; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D008970:Molecular Weight; D011092:Polyethylene Glycols; D011766:Pyruvate Carboxylase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D051381:Rats
D001360:Avidin; D011092:Polyethylene Glycols; D011766:Pyruvate Carboxylase; D000645:Ammonium Sulfate