Effect of time and temperature on blood P50 and 2,3-diphosphoglycerate measurements.
The oxygen affinity of hemoglobin is affected by various factors, including blood pH and erythrocyte 2,3-diphosphoglycerate. The latter and blood P50 values are unchanged when aliquots of blood from normal individuals are permitted to stand at room temperature for as long as 7 h. Our data also show that equilibration in a 37 degrees C tonometer for times ranging from 0.5 to 1.5 h causes no detectable alterations in P50 values.
D000328:Adult; D001784:Blood Gas Analysis; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis; D005260:Female; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010100:Oxygen; D011485:Protein Binding; D013696:Temperature; D013997:Time Factors
D004163:Diphosphoglyceric Acids; D006454:Hemoglobins; D010100:Oxygen