Immunosensitivity and histocompatibility antigens in drug-altered leukemic cells.
New antigenic properties of experimental lymphomas have been reported previously following in vivo treatment with antitumor agents. 5-(3,3-Dimethyl-1-triazeno)imidazole-4-carboxamide (DIC) induced new antigenic characteristics on L1210 and L5178Y lymphomas, that were previously investigated in studies in animals compatible with the original untreated parental tumors. Here the L1210/DIC and L5178Y/DIC susceptibility to the cytotoxic effects of allogeneic and xenogeneic lymphocytes and sera obtained from animals sensitized to DBA/2 histocompatibility antigens were studied. The original and the DIC tumors showed the same sensitivity to anti-DBA/2 cellular and humoral cytotoxicity. The immune response electied in allogeneic mice by the original and DIC sublines was evaluated by in vitro cell-mediated and humoral cytotoxic assay. Beyond the immune response to histocompatibility antigens, a specific, anti-DIC-antigen immunoreaction was not found. Inhibition assay of the cell-mediated cytotoxicity and absorption of the humoral cytotoxicity demonstrated that DIC-induced antigens are not reciprocally related in cell-surface concentration to the natural DBA/2 histocompatibility antigens associated with tumor cells of DIC lines. An experiment was conducted in which specific activity against the DIC-treated L5178Y/DIC cells was observed with anti-L5178Y/DIC rabbit immune serum absorbed with the parental L5178Y lymphoma. This finding provides additional support to previous studies indicating that treatment with DIC induced new antigens on the lymphoma cells.
D000818:Animals; D000917:Antibody Formation; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D003606:Dacarbazine / Q000276:immunology / Q000494:pharmacology*; D006649:Histocompatibility Antigens; D007939:Leukemia L1210; D007942:Leukemia, Experimental / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D014226:Triazenes / Q000494:pharmacology*
D000951:Antigens, Neoplasm; D006649:Histocompatibility Antigens; D014226:Triazenes; D003606:Dacarbazine