Reptilase clot retraction induced by electrical stimulation.
Retraction of platelet rich plasma clotted by reptilase is induced by electrical stimulation. Optimal retraction is obtained by stimuli, applied for more than 4 min, with the following characteristics: intensity = 150 volts, duration = 50 msec each, frequency = 10/sec. Electrically induced reptilase clot retraction is shown to be inhibited by EDTA, EGTA, methyl-xanthines, PGE1, acetylsalicylic acid, indomethacin, but not by apyrase or by phosphoenolpyruvate-pyruvate kinase and MgCl2. The results indicate that electrical stimulation induces retraction of PRP clotted by reptilase by triggering off an increased availability of Ca2+ in the intracellular space.
D001081:Apyrase / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D012103:Batroxobin; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000494:pharmacology; D003018:Clot Retraction; D004558:Electric Stimulation; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D010447:Peptide Hydrolases; D011188:Potassium / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011770:Pyruvate Kinase / Q000494:pharmacology; D013997:Time Factors; D014970:Xanthines / Q000494:pharmacology
D011458:Prostaglandins E; D014970:Xanthines; D011770:Pyruvate Kinase; D010447:Peptide Hydrolases; D012103:Batroxobin; D001081:Apyrase; D008274:Magnesium; D001241:Aspirin; D011188:Potassium; D002118:Calcium; D007213:Indomethacin