Purification of xylanase from the wood-rotting fungus Trametes hirsuta.
Xylanase (EC 3.2.1.8) was purified from a crude extracellular enzyme of the wood-rotting fungus Trametes hirsuta by a four-step procedure involving precipitation with ammonium sulphate, gel filtration on Bio-Gel P-10, column chromatography on DEAE-Sephadex A-50 and preparative electrophoresis on polyacrylamide gel. The isolated enzyme, electrophoretically homogeneous, was separated from beta-xylo-sylosidase and other hydrolytic enzymes studied. The analysis of the degradation products of water-soluble (4-0-methylglucurono)-D-xylan from willow by the purified xylanase showed it to be endoxylanase (beta-1,4-xylan xylanohydrolase).
D000681:Amylases / Q000378:metabolism; D001487:Basidiomycota / Q000201:enzymology*; D002474:Cell-Free System; D002480:Cellulase / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D008361:Mannosidases / Q000378:metabolism; D011124:Polyporaceae / Q000201:enzymology* / Q000378:metabolism; D014934:Wood; D014990:Xylans / Q000378:metabolism
D014990:Xylans; D006867:Hydrolases; D000681:Amylases; D005959:Glucosidases; D006026:Glycoside Hydrolases; D008361:Mannosidases; D002480:Cellulase