[Preparation of a standardized antigenic extract from several strains of African trypansomes].
The preparation of a soluble antigen from african trypanosomes is described below. It is important to observe the following steps: --minimal dose of the phytohemagglutinin must be placed in rapid contact with the trypanosomes; --the standard speed of the different centrifugations must be strictly observed; --a thorough washing of the trypanosomes must be carried out to eliminate the hetero-antigens, but caution should be excercised, as over washing is harmful to the Trypanosomes.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification* / Q000592:standards; D002498:Centrifugation / Q000379:methods; D037102:Lectins; D008722:Methods; D051379:Mice; D051381:Rats; D014346:Trypanosoma brucei brucei / Q000276:immunology*; D014347:Trypanosoma brucei gambiense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000175:diagnosis
D000941:Antigens; D037102:Lectins
Préparation d'un extrait antigénique standardisé à partir de diverses souches de trypansomes africains