title: [Isolation and properties of several auxotrophic mutants of a highly virulent strain of the plague microbe].
abstract: 2432 stable auxotrophic mutants were selected from high virulent Yersinia pestis strain 20b after treatment with nitroso guanidine. They were deficient in amino acids (arginine, aspartic acid, citrulline, glycine, glutamic acid, histidine, isoleucine, serine, leucine, lysine, ornithine, proline, tryptophan, tyrosine, valine), pyrimidine and vitamins (riboflavin, thyamine, nicotinamide). Some mutants were two- and three-fold dependent. The leucine-, histidine-, purine-dependent mutants were isolated with the high frequency. All the mutants, like their original strain, grew in R-form; they were sensitive to diagnostic phages, had pesticine-fibrinolysin-coagulase system (fraction I) and were calcium-dependent. P+ cultures of auxotrophs were not virulent for laboratory animals.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009154:Mutation; D009604:Nitrosoguanidines / Q000494:pharmacology*; D011743:Pyrimidines / Q000378:metabolism; D014774:Virulence; D014815:Vitamins / Q000378:metabolism; D015010:Yersinia pestis / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity
chemical_list: D000596:Amino Acids; D009604:Nitrosoguanidines; D011743:Pyrimidines; D014815:Vitamins; D008769:Methylnitronitrosoguanidine
vernacular_title: Vydelenie i svoĭstva nekotorykh auksotrofnykh mutantov vysokovirulentnogo shtamma chumnogo mikroba