[A simple thin-layer chromatographic assay of urocanic acid in the stratum corneum for evaluation of skin histidase activity (author's transl)].
A simple and rapid urocanic acid estimation by mean of TLC as an indirect assay for skin histidase activity is described. Very small amounts of stratum corneum (about 1 mg) are eluated in concentrated NH3, 5 mul of the eluate were chromatographied and stained with Pauly's reagent. Investigations were done in 76 healthy persons, 17 heterocygotes and 24 children with histidinemia. In 18 histidinemic children urocanic acid was found not at all, in 5 as a trace and in 1 - at the age of 24-28 months. - as a nearly normal concentration. In 11 children with histidinemia and 3 controls the results of our method are compared with these of direct enzyme activity essay and a good correlation could be shown. Identification of heterocygous is not possible.
D000642:Ammonia-Lyases / Q000378:metabolism*; D002675:Child, Preschool; D002855:Chromatography, Thin Layer / Q000379:methods; D006579:Heterozygote; D006639:Histidine / Q000097:blood / Q000652:urine; D006638:Histidine Ammonia-Lyase / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000378:metabolism*; D007223:Infant; D012867:Skin / Q000201:enzymology* / Q000378:metabolism; D014560:Urocanic Acid / Q000378:metabolism*
D007093:Imidazoles; D006639:Histidine; D000642:Ammonia-Lyases; D006638:Histidine Ammonia-Lyase; D014560:Urocanic Acid
Ein einfacher Dünnschiectchromatographic-Nachweis von Urocaninsäure in stratum coreneum als Ausdruck der Aktivität von Hauthistidase