Production and characterisation of antisera to 18-hydroxy-11-deoxycorticosterone.
The production of highly sensitive and specific antisera to 18-hydroxy-11-deoxycorticosterone (18,21-dihydroxy-4-pregnene-3,20-dione) is reported. The antisera were generated in rabbits and guinea pigs with a 3-carboxymethoxime derivative of the steroid coupled to rabbit serum albumin. Antibody characteristics were determined by a radioimmunoassay procedure. Only minor differences between the two animal species were observed. Antibody titers ranged from 10 to 8000. Association constants were in the order of 10(8) to 10(10) 1/mole. A minimal amount of 40 pg unlabeled steroid was necessary to displace 50% of the tritiated steroid. Cross reaction with cortisol was 0.0002% to 0.031%, with aldosterone 0.0007% to 1.09%, with corticosterone 0.0025% to 1%, with 18-hydroxy-corticosterone 0.05% to 1% and with progesterone 0.0048% to 1.5%.
D015070:18-Hydroxydesoxycorticosterone / Q000032:analysis / Q000276:immunology*; D000818:Animals; D003900:Desoxycorticosterone / Q000031:analogs & derivatives*; D006168:Guinea Pigs / Q000276:immunology; D007106:Immune Sera; D008832:Microchemistry; D011233:Precipitin Tests / Q000379:methods; D011817:Rabbits / Q000276:immunology; D013045:Species Specificity
D015070:18-Hydroxydesoxycorticosterone; D007106:Immune Sera; D003900:Desoxycorticosterone