title: Artificial induction of lactation in ewes: the role of prolactin.
abstract: The mammary glands of 30 non-pregnant, intact ewes were developed by subcutaneously injecting oestrogen plus progesterone at intervals of 3 days from day 0 to day 27. Two days later (day 29), 15 ewes were injected subcutaneously with 18 mg ergocryptine, to inhibit specifically secretion of prolactin. Then groups of ewes, each comprising five ergocryptiine-treated and five untreated ewes, were injected from days 30 to 34 with either four intravenous injections each day of 1 i.u. syntocinon, one subcutaneous injection each day of 10 mg dexamethasone trimethylacetate, or two subcutaneous injections each day of 2-5 mg oestradiol benzoate plus 6-25 mg progesterone. All ewes were milked by hand on days 30-50. Within 24 h of injecting ergocryptine, levels of prolactin in serum were reduced to negligible values (less than 2 ng/ml). Comparison of results for ewes not receiving ergocryptine showed that syntocinon, dexamethasone and oestradiol benzoate plus progesterone, at the doses used, were equally effective in initiating milk secretion. Peak yields of 0-23-0-27 kg/day were achieved. On the other hand, ewes treated with ergocryptine before syntocinon or dexamethasone produced peak yields of only 0-12-0-13 kg/day and ewes treated with ergocryptine before oestradiol benzoate plus progesterone produced negligible amounts of secretion. The results suggest that syntocinon and dexamethasone were either lactogenic per se or effected the release of hormones of the lactogenic complex other than prolactin. However, oestradiol benzoate plus progesterone appeared to be lactogenic by virtue of the influence of oestrogen on the secretion of prolactin.
mesh_terms: D000818:Animals; D004873:Ergolines / Q000494:pharmacology*; D005260:Female; D007774:Lactation / Q000187:drug effects*; D007785:Lactose / Q000032:analysis; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000254:growth & development; D008892:Milk / Q000032:analysis / Q000378:metabolism; D011247:Pregnancy; D011388:Prolactin / Q000037:antagonists & inhibitors / Q000097:blood / Q000502:physiology*; D012756:Sheep / Q000502:physiology*
chemical_list: D004873:Ergolines; D011388:Prolactin; D007785:Lactose