[Beta-Hydroxybutyrate dehydrogenase of rat liver inner mitochondrial membrane. Its isolation, characterization, and reactivation by lecithins differing in their apolar regions. The influence of the addition of cholesterol on its level of reactivation (author's transl)].
The beta-hydroxybutyrate dehydrogenase has been isolated and purified from the inner mitochondrial membrane of the rat liver. It consists in a dimer of molecular weight 77 000 composed by two subunits of molecular weight 38 000 each. The level of its reactivation by lecithin is influenced by the length and degree of unsaturated of their aliphatic chains. The addition of cholesterol inhibits the reactivation.
D000818:Animals; D002784:Cholesterol / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism*; D046911:Macromolecular Substances; D008930:Mitochondria, Liver / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000494:pharmacology; D051381:Rats; D013329:Structure-Activity Relationship
D046911:Macromolecular Substances; D010713:Phosphatidylcholines; D002784:Cholesterol; D006884:Hydroxybutyrate Dehydrogenase
La beta-hydroxybutyrate déshydrogenase de la membrane interne des mitochondries de foie de rat. Son isolément, ses caracteristiques, sa réactivation par des lécithines presentant des groupements apolaires differents: influence de l'adjonction de cholestérol sur son degré de réactivation