[Ultrahistochemical demonstration of glucose-6-phosphatase in hair cells of the guinea pig organ of corti (author's transl)].
This study is concerned with the ultrahistochemical demonstration of glucose-6-phosphatase activity and its localization in inner and outer hair cells of the guinea pig organ of Corti. The enzyme activity has been demonstrated by a method according to Hugon et al. (1970). In hair cells of the organ of Corti a characteristic distribution pattern of reaction products has been registered. Subsurface cisterns and the Hensen's bodies of outer hair cells were heavily loaded with reaction products. In addition, the endoplasmic reticulum and the nuclear membrane as well as postsynaptic cisterns were rich in precipitates. With regard to their morphologic pecularities the inner hair cells show a more homogeneous distribution of enzyme activity. The findings corroborate the former assumption of a genetic relationship of either subsurface cisterns and Hensen's body to the endoplasmic reticulum of outer hair cells. Furthermore, the high glucose-6-phosphatase activity of both subsurface cisterns and Hensen's bodies are considered indicative of their participitation in the energy metabolism of outer hair cells. Referring to biochemical studies of Thalmann and associates (1973), the narrow spatial relationship of glucose-6-phosphatase positive ER membranes to mitochondria presumably represents a morphologic correlation of aerobic and anaerobic metabolic pathways in the guinea pig organ of Corti.
D000818:Animals; D004734:Energy Metabolism; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis*; D006168:Guinea Pigs; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009925:Organ of Corti / Q000201:enzymology*
D005954:Glucosephosphate Dehydrogenase
Ultrahistochemische Verteilung der Glucose-6-Phosphatase in den Sinneszellen des Corti-Organs des Meerschweinchens