[Effect of thiamine, riboflavin and ascorbic acid on tetracycline, erythromycin and levomycetin activity].
The effect of antibiotics was estimated by inhibition of the protein increase in the broth culture of Staph, aureus during incubation at a temperature of 37 degrees for 18 hours. In some experiments preincubation of the antibiotic solutions with the vitamins for 2 hours at light and in dark was used. The antibiotic concentrations in gamma per 1 ml were equal to those of the vitamins. In the experiments with tetracycline and 2-hour preincubation at light the antibiotic in a concentration of 0.1gamma ml inhibited for certain the protein increase by 58.9%, in combination with thiamin it inhibited the protein by 60 per cent and in combination with ascorbic acid by 59%. Riboflavin lowered the activity of tetracycline to a value not differing for certain from the control one. In the experiments with preincubation in dark tetracycline inhibited the protein increase by 55.2%, in combination with thiamin it inhibited the protein increase by 50.5%, in combination with riboflavin by 53% and in combination with ascorbic acid by 57.2%. Erythromycin in a concentration of 0.03gamma/ml when preincubated at light inhibited the protein increase by 48.8% and in combinations with thiamin, riboflavin or ascorbic acid by 23, 27, 47.2% respectively. When preincubated in the darkness erythromycin alone inhibited the protein increase by 47.8% and in combinations with thiamin, riboflavin or ascorbic acid by 32.5, 51.1 or 49.8% respectively. The above vitamins has no effect on levomycetin activity.
D001205:Ascorbic Acid / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors; D002701:Chloramphenicol / Q000494:pharmacology*; D003470:Culture Media; D003864:Depression, Chemical; D004347:Drug Interactions; D004917:Erythromycin / Q000494:pharmacology*; D012256:Riboflavin / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology*; D013831:Thiamine / Q000494:pharmacology*
D001426:Bacterial Proteins; D003470:Culture Media; D004917:Erythromycin; D002701:Chloramphenicol; D013752:Tetracycline; D001205:Ascorbic Acid; D012256:Riboflavin; D013831:Thiamine
Vliianie tiamina, riboflavina i askorbinovoĭ kisloty na aktivnost' tetratsiklina, éritromitsina i levomitsetina