The 7.1 S nuclear DNA polymerase and DNA replication in intact liver.
Just as after 70% hepatectomy, the activity of the 7.1 S DNA polymerase, but not the 3.2 S polymerase, is elevated in liver nuclei from unoperated animals in which hepatic DNA replication has been induced with a mixture of biochemicals or by a dietary manipulation. Again as with regenerating liver, the stimulated intact livers show a relationship between the increases in the enzyme activity and thymidine incorporation in vivo over a wide range of hepatic responses. These observations are consistent with a role for the 7.1 S activity in nuclear DNA replication. Cytosine arabinoside 5'-triphosphate and novobiocin can be used to distinguish between the 3.2 S and 7.1 S polymerases from nuclei of stimulated intact liver as well as of regenerating liver.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D003561:Cytarabine / Q000031:analogs & derivatives; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004261:DNA Replication; D005260:Female; D006498:Hepatectomy; D008099:Liver / Q000201:enzymology*; D009675:Novobiocin / Q000494:pharmacology; D051381:Rats; D013095:Spermidine / Q000494:pharmacology; D013268:Stimulation, Chemical; D013936:Thymidine / Q000378:metabolism; D013963:Thyroid Hormones / Q000494:pharmacology
D013963:Thyroid Hormones; D003561:Cytarabine; D009675:Novobiocin; D004254:DNA Nucleotidyltransferases; D013095:Spermidine; D013936:Thymidine