Value of a new technique of sensitization to dinitro-2, 4-chlorobenzene.
Experimental sensitization to dinitro-2,4-chlorobenzene (DNCB) is being studied according to different techniques, the variable factors being the concentrations of DNCB, the number of applications and the time between the sensitizing application and the reading of the challenge test. The authors relate and discuss their technique (only one touch of a drop of 10% DNCB in acetone, covered with a cover-slip and left to evaporate), the results and the advantages: in particular, stanardization of the parameters of sensitization, (quantity of DNCB, area and time of application) and reduction in the risk of toxic effects inherent in excessively high concentrations of DNCB. In addition, the authors emphasize the fundamental significance of the length of time needed to acquire experimental sensitization.
D000328:Adult; D000368:Aged; D003877:Dermatitis, Contact / Q000175:diagnosis* / Q000209:etiology; D004137:Dinitrochlorobenzene / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009578:Nitrobenzenes / Q000276:immunology*; D010328:Patch Tests / Q000379:methods*; D012882:Skin Tests / Q000379:methods*; D013997:Time Factors
D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes