Inhibition of 6-methylsalicyclic acid synthesis by the antibiotic cerulenin.
Cerulenin, a specific inhibitor of beta-ketoacyl-(acyl carrier protein) synthetase [EC 2.3.1.41] and 3-hydroxy-3-methylglutaryl-CoA synthetase [EC 4.1.3.5], was studied to determine whether it inhibits 6-methylsalicylic acid synthesis, in which so-called "polyketide" formation, a condensation step similar to that in fatty acid synthesis, is involved. In fact, 100 mug/ml (4.5 X 10(-4) M) of cerulenin inhibited 60% of 6-methylsalicylic acid synthetase activity and 68% of fatty acid synthetase activity of Penicillium urticae.
D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / Q000037:antagonists & inhibitors*; D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D000935:Antifungal Agents / Q000494:pharmacology*; D002569:Cerulenin / Q000494:pharmacology*; D064429:Fatty Acid Synthases / Q000378:metabolism; D007700:Kinetics; D010407:Penicillium / Q000187:drug effects / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism; D012459:Salicylates / Q000096:biosynthesis*
D000935:Antifungal Agents; D010636:Phenols; D012459:Salicylates; D002569:Cerulenin; D000217:Acyltransferases; D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; D064429:Fatty Acid Synthases