Effects of selected sulfhydryl inhibitors on nonhistone chromosomal proteins of HeLa cells.
Effects of the SH inhibitor sodium iodoacetate, alone and with adjuncts menadiol diphosphate, sodium malonate, sodium fluoride and heparin, on incorporation of tryptophane-3 H into nonhistone chromosomal proteins of HeLa cells were examined. The drugs block incorporation of tryptophane-3 H into nonhistone chromosomal proteins far more than incorporation of leucine-3 H into total cellular proteins. Drug effects on thymidine phosphorylation and DNA synthesis in HeLa cells exceed corresponding effects on fibroblasts from normal healing wounds.
D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005347:Fibroblasts; D005459:Fluorides / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects* / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008314:Malonates / Q000494:pharmacology; D008466:Mechlorethamine / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009698:Nucleoproteins / Q000096:biosynthesis*; D014364:Tryptophan / Q000378:metabolism; D014812:Vitamin K / Q000494:pharmacology
D004273:DNA, Neoplasm; D007461:Iodoacetates; D008314:Malonates; D009363:Neoplasm Proteins; D009698:Nucleoproteins; D014812:Vitamin K; D008466:Mechlorethamine; D014364:Tryptophan; D006493:Heparin; D004254:DNA Nucleotidyltransferases; D007930:Leucine; D005459:Fluorides