[The Km of malate dehydrogenase from pig heart with oxaloacetate as substrate (author's transl)].
Km is necessary to calculate the conditions for indicator reactions in coupled enzymic assays. When malate dehydrogenase is used as an indicator enzyme for the assay of aspartate aminotransferase activity, its Km in relation to oxaloacetate is needed. Km (oxaloacetate) of commercially available mitochondrial malate dehydrogenase from pig heart was determined as Km equals 1.65 x 10(-5) mol/1 using the measurement conditions for aspartate aminotransferase according to the preliminary recommendations of the IFCC.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D010071:Oxaloacetates; D013552:Swine
D010071:Oxaloacetates; D008291:Malate Dehydrogenase; D001219:Aspartate Aminotransferases
Km von Malat-Dehydrogenase aus Schweineherz in bezug auf oxalacetat