A novel fluorimetric determination of sphingosine.
A fluorimetric method to determine sphingosine and the related bases at nmole level is described. For the fluorescence labeling of the long chain bases, pyridoxal was conjugated to the amino groups of the compounds. The method is applicable to sphingolipids after hydrolysis or methanolysis even in the presence of largeamount of contaminating amino-containing substances.
D005470:Fluorometry / Q000379:methods; D013110:Sphingosine / Q000032:analysis*
D013110:Sphingosine