Demonstration of kinetoplast DNA in dyskinetoplastic strains of Trypanosoma equiperdum.
4,6 Diamidino-2-phenylindole (DAPI) forma a highly fluorescent complex with DNA which allows detection of mitochondrial DNA (K-DNA) in normal and dyskinetoplastic strains of Trypanosoma equiperdum. The K-DNA DAPI complexes in the dyskinetoplastic cells, cells lacking detactable K-DNA by other cytochemical methods, are not restricted to a single region of the organism as in the normal strain, but are seen as a row of particles. These observations support the hypothesis that the K-DNA is retained in dyskinetoplastic cells.
D004272:DNA, Mitochondrial / Q000032:analysis*; D005407:Flagella; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D009154:Mutation; D014345:Trypanosoma / Q000648:ultrastructure*
D004272:DNA, Mitochondrial; D005456:Fluorescent Dyes