Patulin inhibition of mycovirus replication in Penicillium stoloniferum.
Penicillium stoloniferum NRRL5267 contains two electrophoretically distinct viruses (PsV-F and PsV-S). An in vivo system was developed to test whether a number of fungal metabolites had antiviral properties on PsV-F replication in O.erties on PsV-F replication in P. stoloniferum. Preliminary results indicated that the mycotoxin patulin can block mycovirus replication. Portions of 48 h mycelium were incubated in the presence of varying levels of patulin, and after an additional 48 h incubation, PsV-F content was measured in E260 units by polyacrylamide gel electrophoresis. Patulin at 11, 16 and 20 mug/mg dry wt mycelia blocked PsV-F replication 26, 61 and 71%, respectively, compared with untreated controls. At these levels, host biomass RNA and protein synthesis were minimally affected. No-proliferating fungal mycelium is capable of continued support of PsV-F replication, which is sensitive to patulin. Apparently, inhibitory doses of patulin stimulated PsV-S replication during this 48 h incubation. The preferential action of patulin may arise from metabolite binding to functional enzymes required for virus replication.
D004305:Dose-Response Relationship, Drug; D005656:Fungal Proteins / Q000096:biosynthesis; D010365:Patulin / Q000494:pharmacology*; D010407:Penicillium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011714:Pyrans / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D012328:RNA Viruses / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
D005656:Fungal Proteins; D011714:Pyrans; D012313:RNA; D010365:Patulin