Chromatin model calculations: Arrays of spherical nu bodies.
Chromatin fibers consists of globular nucleohistone particles (designated nu bodies) along the length of the chromatin DNA with approximately 6-to7-fold compaction of the DNA within the nu bodies. We have calculated theoretical small-angle x-ray scattering curves and have compared these with experimental data in the literature. Several models predict maxima at the correct angles. The first maximum (approximately 110 degrees A) results from interparticle interference, while both the spatial arrangement and the structure factor the nu bodies can contribute to the additional small-angle maxima. These calculations suggest models which can account for the electron microscopic observation that chromatin is seen as either approximately 100-or approximately 200-to 250 degrees A-diameter fibers, depending on the solvent conditions. They also account for the limited orientability of the x-ray pattern from pulled chromatin fibers.
D001665:Binding Sites; D002843:Chromatin / Q000648:ultrastructure; D004247:DNA; D008433:Mathematics; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D014961:X-Ray Diffraction
D002843:Chromatin; D004247:DNA