Short-term treatment of alloxan-diabetic rats with intrajejunal administration of water-in-oil-in-water insulin emulsions.
Alloxan-diabetic rats with fasting blood glucose levels above 300 mg./100 ml. were treated with intrajejunal administration of water-in-oil-in-water (W/O/W) insulin emulsions via an indwelling catheter at a dose of either 25 or 50 U./100 gm. body weight, three times daily for five to fourteen days. The course of diabetes was followed by determinations of glucose levels in blood and urine. During treatment a significant reduction in urinary glucose levels was observed in all rats studied. In two rats treated with 25 U./100 gm., fasting blood glucose levels did not change significantly. In four of five rats treated with 50 U./100 gm., W/O/W insulin emulsions significantly lessened hyperglycemia during treatment. Quantitative estimates suggested that the effectiveness of 50 U./100 gm. of intrajejunal W/O/W insulin emulsions was comparable to that after intramuscular regular insulin at doses between 1 and 2 U./100 gm. These results would indicate that diabetes can be controlled by enteral administration of insulin preparations.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002404:Catheterization; D002940:Circadian Rhythm; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D005069:Evaluation Studies as Topic; D006029:Glycosuria; D007273:Injections, Intramuscular; D007328:Insulin / Q000008:administration & dosage / Q000627:therapeutic use*; D007583:Jejunum; D008297:Male; D009821:Oils; D051381:Rats; D013997:Time Factors; D014867:Water
D001786:Blood Glucose; D004655:Emulsions; D007328:Insulin; D009821:Oils; D014867:Water