title: The protein-mediated transfer of phosphatidylcholine between membranes. The effect of membrane lipid composition and ionic composition of the medium.
abstract: The phosphatidylcholine exchange protein from bovine liver catalyzes the transfer of phosphatidylcholine between rat liver mitochondria and sonicated liposomes. The effect of changes in the liposomal lipid composition and ionic composition of the medium on the transfer have been determined. In addition, it has been determined how these changes affected the electrophoretic mobility i.e. the surface charge of the membrane particles involved. Transfer was inhibited by the incorporation of negatively charged phosphatidic acid, phosphatidylserine, phosphatidylglycerol and phosphatidylinositol into the phosphatidylcholine-containing vesicles; zwitterionic phosphatidyl-ethanolamine had much less of an inhibitory effect while positively charged stearylamine stimulated. The cation Mg2+ and, to a lesser extent, K+ overcame the inhibitory effect exerted by phosphatidic acid, in that concentration range where these ions neutralized the negative surface charge most effectively. Under conditions where Mg2+ and K+ affected the membrane surface charge relatively little inhibition was observed. In measuring the protein-mediated transfer between a monolayer and vesicles consisting of only phosphatidylcholine, cations inhibited the transfer in the order La3+ greater than Mg2+ larger than or equal to Ca2+ greater than K+ = Na+. Inhibition was not related to the ionic strength, and very likely reflects an interference of these cations with an electrostatic interaction between the exchange protein and the polar head group of phosphatidylcholine.
mesh_terms: D000818:Animals; D001665:Binding Sites; D001693:Biological Transport, Active; D002118:Calcium; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D050356:Lipid Metabolism; D008081:Liposomes / Q000378:metabolism*; D008274:Magnesium; D008297:Male; D008566:Membranes / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines / Q000378:metabolism*; D011188:Potassium; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011955:Receptors, Drug; D013229:Stearic Acids
chemical_list: D008081:Liposomes; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D011506:Proteins; D011955:Receptors, Drug; D013229:Stearic Acids; D008274:Magnesium; D011188:Potassium; D002118:Calcium