Modification of bovine pancreatic ribonuclease A with pyridoxal 5'-phosphate. Isolation and identification of derivatives.
Bovine pancreatic ribonuclease A was allowed to react with pyridoxal 5'-phosphate at pH 8 and 4 degrees. After reduction with sodium borohydride, the principal products formed in the initial stages of modification were separated by successive chromatography on CM-cellulose and SP-Sephadex. The isolated derivatives were identified as Nalpha-(P-pyridoxyl)-Lys-1-,Nepsilon-(P-pyridoxyl)-Lys-7-, and Nepsilon-(P-pyridoxyl)-Lys-41-ribonuclease A. These results are interpreted in terms of the specificity of pyridoxal-P as a protein reagent.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D008239:Lysine / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D010179:Pancreas / Q000201:enzymology; D011732:Pyridoxal Phosphate; D012260:Ribonucleases
D000596:Amino Acids; D011732:Pyridoxal Phosphate; D012260:Ribonucleases; D008239:Lysine