Quantitative evaluation of autoradiographs by X-ray spectroscopy.
Measurements of mass of developed silver in autoradiograms have been made using X-ray microanalysis. This procedure has been applied to a scanning electron microscopic study of protease-induced cell stimulation in 3H-thymidine-labelled bladder tissues in vitro. Differences in silver concentrations over individual nuclei were demonstrated by X-ray analysis and quantitative data was obtained showing an increased rate of 3H-thymidine incorporation in protease-treated cultures. The initial evidence indicates that X-ray microanalysis could provide a potentially useful quantitative procedure for autoradiography.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000648:ultrastructure; D004247:DNA / Q000096:biosynthesis; D004577:Electron Probe Microanalysis; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D010447:Peptide Hydrolases / Q000494:pharmacology; D001743:Urinary Bladder / Q000648:ultrastructure*; D014965:X-Rays
D004247:DNA; D010447:Peptide Hydrolases