An extended kinetic analysis of valinomycin-induced Rb-transport through monoglyceride membranes.
The time course of the current following a voltage jump, which is applied to monoglyceride bilayers in the presence of valinomycin, shows two relaxation times. This is basically in agreement with a simple carrier model which has been described in full detail formerly. Relaxation times and amplitudes allow a calculation of the rate constants of the transport model. The presented data supplement an analysis which was hitherto based only on the slower relaxation process and on information derived from the nonlinearity of current-voltage characteristics. The additional resolution of the faster relaxation time allowed an approximate determination of the voltage dependence of the translocation rate constant for carrier-ion-complex and provided evidence for a small voltage dependence of the interfacial reaction. The dependence of the relaxation parameters on the ion concentration in the aqueous phase was interpreted assuming a saturation of the ion concentration at the reaction plane at high bulk concentrations.
D001692:Biological Transport; D004594:Electrophysiology; D005989:Glycerides; D007700:Kinetics; D008433:Mathematics; D008567:Membranes, Artificial; D008954:Models, Biological; D010716:Phosphatidylinositols; D012413:Rubidium / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014634:Valinomycin / Q000494:pharmacology*
D005989:Glycerides; D008567:Membranes, Artificial; D010716:Phosphatidylinositols; D014634:Valinomycin; D012413:Rubidium