Radioactive antigen suicide of an anti-DNP (2,4-dinitrophenyl) clone. I. Recovery and escape from clonal dominance by suicide resistant precursors.
An anti-2,4-dinitrophenyl (DNP) clone line, maintained in vivo by serial passage through irradiated syngeneic recipients, was exposed to 125I-labeled dinitrophenylated L-Tyr-L-Glu-L-Lys copolymer antigen of high specific activity. Hapten-specific suicide was obtained, though with more difficulty than for a heterogeneous secondary response. The recovery was followed and was shown to occur either with the same clones or with new clones, depending on conditions. After suicide at a late passage of the clone, recovery by new clones occured very rapidly. This indicated that there was a background heterogeneity in the clone system, normally suppressed by clonal dominance and that some of these new clones were suicide resistant.
D000818:Animals; D000921:Antibody-Producing Cells / Q000528:radiation effects*; D002469:Cell Separation; D002999:Clone Cells / Q000276:immunology*; D004136:Dinitrobenzenes / Q000276:immunology; D005260:Female; D006241:Haptens; D007156:Immunologic Memory; D051379:Mice; D008808:Mice, Inbred CBA; D010455:Peptides / Q000276:immunology; D013997:Time Factors
D004136:Dinitrobenzenes; D006241:Haptens; D010455:Peptides