Passive cation movements in the Ehrlich ascites tumor cell: the effects of 2,4,6-trinitrobenzene sulfonic acid.
We have investigated the effects of 2,4,6-trinitrobenzene sulfonic acid (TNBS), an amino reactive reagent, on passive cation movements in Ehrlich ascites tumor cells. Incubation of tumor cells with TNBS (3 mM) results in a two phase association of TNBS with the cells. An initial, rapid phase, presumably at the level of the membrane, is independent of temperature, while the second phase increases linearly in time and is temperature dependent. Kinetic analyses of Na+ movements indicate that TNBS: (1) inhibits Na+ movement from a slowly exchanging cellular compartment, but is without effect on a more rapidly exchanging compartment; (2) does not alter net Na+ accumulation in transport-inhibited cells; and (3) is without effect on non-exchange Na+ efflux at 0 degrees C. The actions of TNBS on K+ movements depend upon temperature and the continued presence of TNBS in the environment. At 22 degrees C two minute exposure of the cells to TNBS leads to 77% inhibition of K+ efflux. With continued exposure to TNBS, the inhibition is only 42%. Reduction of the temperature to 0 degrees C decreases K+ efflux in control cells by 82%. Two minute exposure to TNBS enhances K+ efflux by 50%, while continuous exposure increases it by 144%. These results suggest: (1) TNBS interacts with several classes of membrane sites which are involved with the regulation of passive cation movements; and (2) passive Na+ and K+ movements across the cell membrane proceed by different pathways.
D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009578:Nitrobenzenes / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013696:Temperature; D014302:Trinitrobenzenesulfonic Acid / Q000378:metabolism / Q000494:pharmacology*
D009578:Nitrobenzenes; D014302:Trinitrobenzenesulfonic Acid; D012964:Sodium; D011188:Potassium