Reversible loss in suspension culture of a major cell-surface glycoprotein of the TA3-Ha mouse tumor.
A major cell-surface glycoprotein of the TA3-Ha ascites mammary adenocarcinoma diminished during transfer from ascites growth to cell growth in suspension culture. A sensitive, hemagglutination-inhibition assay that used a lectin from Vicia graminea seeds indicated approximately a 50% loss after 7-10 days of culture and a 90% loss after 2 months. These findings were corroborated by carbohydrate and amino acid analysis with gas-liquid chromatography of trypsin glycopeptides released from the cell surface. Repassage of the cultured cells in vivo caused the reappearance of the surface glycoprotein.
D000230:Adenocarcinoma / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002845:Chromatography; D006023:Glycoproteins / Q000032:analysis*; D006385:Hemagglutination Inhibition Tests; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D013997:Time Factors
D000596:Amino Acids; D006023:Glycoproteins