title: [Identification and characterization of proteins from human bronchial secretion (author's transl)].
abstract: An analysis of bronchial mucus proteins was carried out by crossed immunoelectrophoresis. Before electrophoretic migration, sputum was treated with Ecteola-cellulose, which retains acid mucins. The proteins were then extracted by a phosphate/saline buffer pH 7.5. Crossed immunoelectrophoresis of the "bronchial extracts" was carried out with an anti-human serum: fifteen proteins were detected. Among them, IgA and protease inhibitiors play an important role in bronchial pathology. Bronchial extracts were also studied with immune serums against milk proteins, whole saliva and proteins of bronchial mucus. Bronchotransferrin, amylase and two esterases were characterized. Four other proteins were also detected with immune serums against bronchial mucus-proteins: their biological role is still unknown.
mesh_terms: D000681:Amylases / Q000032:analysis; D001980:Bronchi; D001991:Bronchitis; D004950:Esterases / Q000032:analysis; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000032:analysis; D008894:Milk Proteins / Q000276:immunology; D008895:Milk, Human; D009093:Mucus / Q000032:analysis*; D011247:Pregnancy; D011480:Protease Inhibitors; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D012463:Saliva / Q000276:immunology; D013045:Species Specificity; D014168:Transferrin / Q000032:analysis
chemical_list: D007070:Immunoglobulin A; D008894:Milk Proteins; D011480:Protease Inhibitors; D011506:Proteins; D014168:Transferrin; D004950:Esterases; D000681:Amylases
vernacular_title: Identification et caractérisation des constituants protétiques de la sécrétion bronchique humaine