[Application of a reversed passive hemagglutination test to the detection of HBs antigen].
In this report we present an evaluation of the sensitivity, specificity and ability to detect HBs Ag carriers of a new reversed passive hemagglutination test, using immunochemically purified chimpanzee anti HBs bound to stabilized human erythrocytes. The method was shown to have a sensitivity equal (within one two fold dilution) to that of the Ausria I 125 ratio immuno assay, and in a double blind comparison detected essentially the same number of Hbs Ag containing specimens among volunteer blood donors. The method therefore provides an economical method for the third generation testing of blood donors. The methodology which has been described incorporates a definitive specificity test in which serum drawn before and after immunization of chimpanzees with purified HBs Ag is compared for its ability to neutralize the hemagglutination reaction. The use of serum from the same animal for this purpose avoids the theoretical possibility that antiglobulin antibodies directed at subclass determinants such as Gm of Inv could be differentially inhibited due to possible subclass differences in the blocking sera employed. A reliable test for specificity of HBs Ag screening results is essential to avoid false notification of donors that they are carriers of hepatitis B virus.
D000818:Animals; D001782:Blood Donors; D001800:Blood Specimen Collection / Q000379:methods*; D006386:Hemagglutination Tests / Q000379:methods*; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens / Q000302:isolation & purification*; D006801:Humans
D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens
Technique d'hémagglutination passive inverse appliquée à la détection de l'antigène HBs