Production of immune anti-Lewis sera in goats.
Incomplete anti-Lec, anti-Led, anti-Les (anti-Leb+Led), anti-Lens (anti-Lea+Lec), anti-Lem (anti-Leb+Lec) and anti-Lex (anti-Lea+Leb) antibodies were prepared by the immunization of goats with boiled saliva, absorbing the resulting sera with trypsinized erythrocytes. The discovery (2) of anti-Led which reacts only with Le(a-b-) red cells of secretors, together with an analysis of the theories of GRUBB and CEPPELLINI led the present author to predict the existence of anti-Lec which would react only with Le(a-b-) red cells of non-secretors. GUNSON and LATHAM (1)reported their finding of an antibody fulfilling this latter criterion in the serum of a sample submitted for routine antenatal tests.
D000818:Animals; D000917:Antibody Formation; D006041:Goats / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D007983:Lewis Blood Group Antigens; D012463:Saliva / Q000276:immunology
D007106:Immune Sera; D007983:Lewis Blood Group Antigens