title: Chemical analytic approaches to the definition of uremic toxins.
abstract: In this preliminary research we have started a systematic study of substances present in uremic but non in normal serum. Methylguanidine has been examined by the new single method of ion pair extraction and has been found in higher concentration in uremic plasma than in normal, but in lower concentration than previously described with less selective method and used in experimental animal intoxication. We did not find a correlation of Methylguanidine concentration and uremic symptoms and complications as pericarditis, neuropathy and uremic coma. Using the high spead gel filtration method comparative analyses of uremic and normal fluids and many substances have been registered a 9 peak serie. Peak 7 seem to be the most interesting. It is less prominent in normal plasma, increases in uremic patients and decreases after dietary treatment, peritoneal dialysis and hemodialysis. Furthermore it is formed by middle molecular weight substances (approximately 1300).
mesh_terms: D002850:Chromatography, Gel; D006801:Humans; D008760:Methylguanidine / Q000097:blood; D008970:Molecular Weight; D014118:Toxins, Biological / Q000097:blood*; D014462:Ultrafiltration; D014511:Uremia / Q000097:blood*
chemical_list: D014118:Toxins, Biological; D008760:Methylguanidine