Differential control of rat microsomal "aryl hydrocarbon" monooxygenase and epoxide hydratase.
A growing body of evidence implicates epoxide metabolites of mutagenic and carcinogenic polycyclic hydrocarbons as either the only species, or one of the contributing species responsible for these adverse effects. Selective induction of epoxide hydratase(s) catalyzing the transformation of epoxides to electrophilically unreactive dihydrodiols, under conditions not leading to increases in monooxygenase(s) responsible for epoxide formation would, therefore, be of interest. All inducers of rat hepatic epoxide hydratase (determined with [7-3H]styrene oxide as substrate) which have been discovered also induced monooxygenase (determined with benzo(a)pyrene as substrate) suggesting a possible common biosynthetic control of these enzymes. The enzyme levels observed in different sexes and at different stages of the ontogenetic development, possibly dependent on endogenous inducers, strengthened this view. No sex difference is epoxide hydratase activity was observed in young rats (1 to 5 days old) while epoxide hydratase levels were about 3-fold higher in adult males than in females, which was remarkably similar to the behavior of monooxygenase. Moreover, the prenatal development of epoxide hydratase and monooxygenase appeared to be similar--although the low enzyme levels precluded accurate determinations of the latter. Although different types of known monooxygenase inducers all led to epoxide hydratase induction in adult rat liver, their effect of epoxide hydratase and monooxygenase could be dissociated by transplacental treatment. Dissociation was clearest with inducers of the polycyclic hydrocarbon type which led to great induction of monooxygenase while epoxide hydratase remained unchanged. The increases in monooxygenase activity were very different when determined by two methods based on different principles, demonstrating that at least two monooxygenases are involved in oxidative metabolism of benzo(a)pyrene, and that the control of epoxide hydratase is not under common control with either of them.
D000375:Aging; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D004851:Epoxide Hydrolases / Q000378:metabolism*; D005260:Female; D005333:Fetus; D006836:Hydro-Lyases / Q000378:metabolism*; D008099:Liver / Q000254:growth & development; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D009153:Mutagens; D009570:Nitriles / Q000494:pharmacology; D010634:Phenobarbital / Q000494:pharmacology; D011247:Pregnancy; D011284:Pregnenolone / Q000494:pharmacology; D051381:Rats
D009153:Mutagens; D009570:Nitriles; D008748:Methylcholanthrene; D011284:Pregnenolone; D001189:Aryl Hydrocarbon Hydroxylases; D004851:Epoxide Hydrolases; D006836:Hydro-Lyases; D010634:Phenobarbital