Anti-Pr3: serological and immunochemical identification of a new anti-Pr subspecificity.
A monoclonal IgM(kappa) anti-Pr cold agglutinin occurring after a rubella infection is shown to have the 'new' anti-Pr subspecificity anti-Pr3. Pr3 determinants are found on cat and sheep erythrocytes which lack Pr1 and Pr2 determinants. By carbodiimide treatment of human erythrocyte glycoproteins, which causes intramolecular coupling of N-acetylneuraminic acid carboxyl groups and nucleophilic centers of the glycoprotein backbone, Pr3 antigen activity is strongly increased, while Pr1 and Pr2 determinants are inactivated.
D000328:Adult; D000373:Agglutinins / Q000302:isolation & purification*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000302:isolation & purification*; D001789:Blood Group Antigens; D003080:Cold Temperature; D004912:Erythrocytes / Q000276:immunology*; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D008297:Male
D000373:Agglutinins; D001323:Autoantibodies; D001789:Blood Group Antigens; D006023:Glycoproteins; D007075:Immunoglobulin M