Improved method for isolation of human IgM as a by-product.
The authors describe a simple method for isolating heterogeneous IgM from fraction I obtained as a by-product of the large scale fractionation of human plasma. The optimum circumstances of fractionation were determined and it was found that the majority of IgM is precipitated between 2 and 5% polyethylene glycol concentration and 1.1 and 1.8 M ammonium sulphate concentration. After a simple fractionation and gel filtration, a preparation of high purity can be obtained.
D000645:Ammonium Sulfate / Q000494:pharmacology; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D007075:Immunoglobulin M / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000494:pharmacology; D012712:Serum Globulins / Q000032:analysis
D007075:Immunoglobulin M; D012712:Serum Globulins; D011092:Polyethylene Glycols; D005340:Fibrinogen; D000645:Ammonium Sulfate