title: [Concentration and subcellular distribution of iron and copper in the pig liver. II. Studies on the livers of porkers and swine for slaughter with regard to the addition of copper sulfate to the diet].
abstract: In the livers of weaners, an average concentration of storage iron of 69.1 +/- 47.6 mug, and in those of pigs for slaughter, 326 +/- 54 mug/g tissue was measured. Addition of 250 ppm of copper as CuSO4-5H2O to the animals' food led to a significantly lower concentration of 248 +/- 63-5 mg Fe/g liver in pigs for slaughter. Of the storage iron, 66-1% for weaners, 79.2% for pigs for slaughter, and 76% for animals given Cu-supplemented food were localized in the microsomal fraction and in the cytosol. The copper concentration in the liver was 5.2 +/- 0.6 mug/g in weaners, 8.1 +/- 2.4 mug/g in pigs for slaughter and 120 +/- 83 mug/g in animals kept on a Cu-supplemented diet, Of these, 72.6% (pigs for slaughter) and 71.9% (weaners) came to the microsomal fraction and the cytosol, whereas addition of 250 ppm copper to the food ration enhanced the proportion in the nuclear fraction from 7.7 to 29.5%, and in the mitochondrial fraction, from 19.8 to 45.1%. The percent distribution of the copper on the subcellular fractions depends on the copper concentration in the liver: in the range of 5-12 mug/g tissue, increased copper concentration in the liver caused preferential localization of the copper in the cytosol, whereas between 25 and 330 mug/g tissue the copper was stored subcellularly in the following order: mitochondrial, nuclear, cytoplasmatic and microsomal fractions.
mesh_terms: D000818:Animals; D003300:Copper / Q000008:administration & dosage / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D004032:Diet; D005260:Female; D007501:Iron / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008297:Male; D008862:Microsomes, Liver / Q000032:analysis; D008930:Mitochondria, Liver / Q000032:analysis; D013431:Sulfates / Q000008:administration & dosage; D013552:Swine / Q000254:growth & development*
chemical_list: D013431:Sulfates; D003300:Copper; D007501:Iron
vernacular_title: Konzentration und subzelluläre Verteilung von Eisen und Kupfer in der Leber von Schweinen. II. Untersuchungen an der Leber von Läufer- und Schlachtschweinen unter besonderer Berücksichtigung eines Zusatzes von Kupfersulfat zur Futterration