title: [Significance of radioimmune method for detection of HB antigen in blood donors].
abstract: The evaluation of frequency of HBag in 923 blood donors is given: the counterelectrophoresis demonstrates HBag positive cases in 1, 2% the radioimmunoassay (Ausria-125, Abbott) in 1, 5%. The haemagglutination (Hepanosticon, Organon) in 7 cases, which were negative with counterelectrophoresis, and positive with the radioimmunoassay, discovers HBAg in 3 cases. RIA method is more sensitive in the control of HBAg positive donors after two to three years, and in detecting of immuno complexes prepared in vitro. The titre of the antigen is 1000 times higher when made with RIA method, and up to 60 times higher when made with the method of haemagglutination than with the counterelectrophoresis. In 4 cases of hepatitis after transfusion of HBAg negative blood, determined with counterelectrophoresis, have been involved bottles, which were positive only with RIA Method. Austria II-125 (Abbott) method was comparatively tested with counterelectrophoresis on different groups--446 persons.
mesh_terms: D000936:Antigen-Antibody Complex; D001782:Blood Donors; D005260:Female; D006386:Hemagglutination Tests; D006387:Hemagglutination, Viral; D006509:Hepatitis B / Q000276:immunology*; D006511:Hepatitis B Antigens / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D011863:Radioimmunoassay
chemical_list: D000936:Antigen-Antibody Complex; D006511:Hepatitis B Antigens
vernacular_title: Znacaj radioimune metode za testiranje HB antigena kod davalaca krvi