Retarding effect of dodecyl alcohol on polyacrylamide gel electrophoresis of SDS micelles and SDS-protein polypeptide complexes.
Micelles of sodium dodecyl sulfate (SDS) are significantly retarded by the addition of a small amount of dodecyl alcohol to a sample solution in SDS-polyacrylamide gel electrophoresis. The phenomenon can be ascribed to the decrease in charge density due to the incorporation of dodecyl alcohol into SDS micelles. The effect is extended to SDS-protein polypeptide complexes when the amount of SDS micelles is insufficient to accomodate the dodecyl alcohol. A similar effect is likely to occur when SDS-polyacrylamide gel electrophoresis is applied to a sample containing lipophilic materials.
D000438:Alcohols; D001665:Binding Sites; D003102:Colloids; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008823:Micelles; D008970:Molecular Weight; D011485:Protein Binding; D011506:Proteins; D012967:Sodium Dodecyl Sulfate
D000438:Alcohols; D003102:Colloids; D008823:Micelles; D011506:Proteins; D012967:Sodium Dodecyl Sulfate