Electron microscope study on human lymphocyte-sheep erythrocyte rosettes.
Nonimmune rosette formation of human peripheral blood lymphocytes and cultered MOLT 3 and MOLT 4 cells with sheep red blood cells was studied by transmission electron microscopy. At the cell contact area of the rosette, lymphoid and red cell membranes were parallel and 80-100 A apart. The inner leaflet of the lymphoid cell membrane seemed denser, and amorphous substance attached to its cytoplasmic face. The cell contact area was 100-1000 nm wide and frequently on the lymphoiid cell body rather than on microvilli, though some cells extended microvilli near red cells.
D000818:Animals; D002448:Cell Adhesion; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D004912:Erythrocytes / Q000648:ultrastructure; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D008854:Microscopy, Electron; D012756:Sheep