title: Parasexual genetic analysis of aggregation-deficient mutants of Dictyostelium discoideum.
abstract: One hundred and thirty-nine independent, nitrosoguanidine-induced mutants blocked early in development were isolated in two haploid strains of D. discoideum. Forty of these developmental mutants were completely aggregation-deficient on bacterial lawns (Class I mutants) and these mutants were selected for parasexual genetic analysis. By fusing the Class I mutants with developmentally-competent strains the developmental mutations in 39 of these mutants were shown to be recessive; the remaining mutation appeared to be partially dominant. Complementation analysis of the developmental mutations in the Class I strains identified 5 complementation groups. Statistical analysis of the complementation data suggests that there are approximately 40 genes in this organism which will completely block aggregation when mutated and perhaps as many as 150 genes involved in some aspect of the aggregation process. Linkage analysis of 18 Class I developmental mutations revealed that 10 of these mutations map in linkage group II at a minimum of 5 loci.
mesh_terms: D002449:Cell Aggregation; D002874:Chromosome Mapping; D002875:Chromosomes; D004023:Dictyostelium / Q000502:physiology*; D005799:Genes, Dominant; D005808:Genes, Recessive; D008040:Genetic Linkage; D006238:Haploidy; D009154:Mutation; D009235:Myxomycetes / Q000502:physiology*; D009604:Nitrosoguanidines / Q000494:pharmacology; D011995:Recombination, Genetic; D012723:Sex
chemical_list: D009604:Nitrosoguanidines