[Determination of protein concentration in cleaving mammalian ova by the capillary spectrophotometry method].
A micromodification of the Lowry's method is described which allows to measure reliably the protein content in 5-20 embryos of white rats and CBA mice. Differences in the protein content in rat embryos at the stages of 2 blastomeres and blastocyst were shown to be statistically unreliable (20.2 +/- 1.4 and 18.9 +/- 1.3 ng, resp.). The protein content in the mouse embryos at the same two stages differs reliably (19.1 +/- 1.1 and 22.0 +/- +/- 1.7 ng, resp.). The protein content in zona pellucida does not differ reliably from those in rat embryos both at the stages of 2 blastomeres (4.5 ng) and blastocyst (2.3 ng). The protein content in embryos devoid of zona pellucida decreased after 3 hours incubation in the medium 199 at 37 degrees at the stages of morula and blastocyst by 17-20% in rats and by 50% in mice. Addition of 1% serum albumin to the incubation medium did not prevent the partial "loss" of protein by the embryos. The protein content in the rat and mouse embryos at the stage of 2 blastomeres suffered no changes under long-term incubation in the medium 199.
D000818:Animals; D005260:Female; D051379:Mice; D008808:Mice, Inbred CBA; D010063:Ovum / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D013053:Spectrophotometry / Q000379:methods
D011506:Proteins
Opredelenie soderzhaniia belka v drobiashchikhsia iaĭtsekletkakh mlekopitaiushchikh metodom kapilliarnoĭ spektrofotometrii