Preparation and isolation of a taste bud-derived fraction from bovine circumvallate papillae.
A crude extract from taste buds was prepared from circumvallate papillae of bovine tongues by a procedure consisting of freezing, coring, excision, treatment with hypotonic buffer, nitrogen pressurization and selective homogenization. Examination of taste buds by light and electron microscopy before and after this procedure indicated that some of the contents of the buds, mainly from the more apical portions, was extruded following the procedure; anatomical changes could not be observed in the epithelial tissue immediately surrounding the taste buds.
D000818:Animals; D002417:Cattle; D008854:Microscopy, Electron; D013650:Taste Buds / Q000166:cytology* / Q000648:ultrastructure