Studies of biosynthesis of insulin by isolated perfused rat pancreas.
Incorporation studies with 3H-leucine have demonstrated that proinsulin is formed as a precursor of insulin in the perfused pancreas. A dependency of the insulin synthesis on the glucose concentration was revealed, based on a direct stimulation of the proinsulin synthesis. The conversion of proinsulin into insulin was not accelerated by glucose stimulation. The conversion also occurred in the absence of glucose.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000096:biosynthesis*; D007930:Leucine / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D010477:Perfusion; D011384:Proinsulin / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D013268:Stimulation, Chemical; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism
D007328:Insulin; D011691:Puromycin; D011384:Proinsulin; D014357:Trypsin; D007930:Leucine; D005947:Glucose