Some biological properties and an in vivo evaluation of tyrosine phenol-lyase on growth of B-16 melanoma.
Tyrosine phenol-lyase from Erwinia herbicola was purified with the goal of assessing its effect on growth of malignant melanoma. Ammonium sulfate-sodium citrate fractionation and diethylaminoethyl cellulose-hydroxylapatite chromatography were used. The purified enzyme was shown to reduce plasma tyrosine levels when administered to normal C57BL x DBA/2 F1 mice. The plasma half-life value of the enzyme was found to be 6 to 7 hr. Unlike results reported with glutaminase and asparaginase preparations, the lactate dehydrogenase-elevating virus had no significant influence on plasma clearance of tyrosine phenol-lyase. The enzyme significantly inhibited growth of established B-16 melanoma tumors.
D000818:Animals; D005260:Female; D007772:Lactate dehydrogenase-elevating virus; D008190:Lyases / Q000627:therapeutic use*; D008297:Male; D008545:Melanoma / Q000188:drug therapy*; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy; D010649:Phenylalanine / Q000097:blood; D014443:Tyrosine / Q000097:blood; D014447:Tyrosine Phenol-Lyase / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*
D014443:Tyrosine; D010649:Phenylalanine; D008190:Lyases; D014447:Tyrosine Phenol-Lyase