title: Evaluation of methods of coenzyme activation of erythrocyte enzymes for detection of deficiency of vitamins B1, B2, and B6.
abstract: We describe optimized, ultraviolet spectrophotometric procedures for determination of erythrocyte transketolase, glutathione reductase, and aspartate aminotransferase activity, and their activation by their respective coenzymes--thiamine pyrophosphate, flavin-adenine dinucleotide, and pyridoxal-5-phosphate--as tests for vitamin B1, B2, and B6 deficiency. With these procedures we have investigated healthy subjects on normal and vitamin-supplemented diets, and a series of (mainly) alcoholic hospital in-patients. The enzyme procedures described have good precision and can be readily carried out in the routine laboratory. Abnormal transketolase activation correlated well with clinical evidence of vitamin B1 deficiency.
mesh_terms: D001219:Aspartate Aminotransferases / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D005182:Flavin-Adenine Dinucleotide; D005980:Glutathione Reductase / Q000097:blood*; D007700:Kinetics; D011732:Pyridoxal Phosphate; D012257:Riboflavin Deficiency / Q000175:diagnosis / Q000201:enzymology; D013832:Thiamine Deficiency / Q000175:diagnosis / Q000201:enzymology; D013835:Thiamine Pyrophosphate; D014174:Transketolase / Q000097:blood*; D026681:Vitamin B 6 Deficiency / Q000175:diagnosis / Q000201:enzymology; D014804:Vitamin B Deficiency / Q000175:diagnosis*
chemical_list: D005182:Flavin-Adenine Dinucleotide; D011732:Pyridoxal Phosphate; D005980:Glutathione Reductase; D014174:Transketolase; D001219:Aspartate Aminotransferases; D013835:Thiamine Pyrophosphate