Post-replication repair of DNA in Chinese hamster cells treated with cis platinum (II) diamine dichloride. Enhancement of toxicity and chromosome damage by caffeine.
The anti-tumor agent cis platinum (II) diammine dichloride (cis Pt(II)) caused chromosomal abnormalities in Chinese hamster V79-379A cells. The time of appearance of these abnormalities suggested that they arise as a consequence of DNA synthesis on a damaged template. The yield and severity of chromosomal abnormalities was greatly enhanced by a non-toxic concentration of caffeine, and this enhancement was associated with a potentiation of cis Pt(II) induced cell death. These results suggest that damage to DNA which arises from cis Pt(II) treatment can be repaired in this cell line by a caffeine-sensitive post-replication repair process.
D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002460:Cell Line; D002869:Chromosome Aberrations; D002945:Cisplatin / Q000633:toxicity*; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair; D004347:Drug Interactions
D002110:Caffeine; D004247:DNA; D002945:Cisplatin