Concanavalin A as a selective agent in tissue culture for temperature-sensitive hamster cell lines.
A novel method for isolating Chinese hamster cell cultures with temperature-sensitive growth properties is described. Concanavalin A can be used as a selective agent in tissue culture to isolate lectin-resistant cell lines which exhibit colony-forming abilities at the nonpermissive temperature relative to the permissive temperature as low as 10(-4) to 10(-6). A general correlation exists between resistance to the lectin and temperature sensitivity.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D006224:Cricetinae; D004351:Drug Resistance; D005260:Female; D037102:Lectins / Q000494:pharmacology; D010053:Ovary; D013696:Temperature
D037102:Lectins; D003208:Concanavalin A