Studies on 5-hydroxyuridine (isobarbituridine): X. Isolation and tentative identification of 5-hydroxymethyluridine from ribosomal RNA.
Yeast ribosomal RNA was hydrolyzed to its constituent nucleosides with the aid of snake venom and bacterial alkaline phosphatase. Lyophilized hydrolysate was labeled with radioactive 5-hydroxyuridine and applied to partition chromatography. It was found that some components of rRNA are held on the column and can be eluted with water. Eighty-nine percent of the label, a large portion of cytidine, and several unidentified compounds were found in the water wash. The direct application of the wash concentrate to ascending paper chromatography in saturated butanol-H2O resulted in the separation of three distinct UV-absorbing bands. Further resolution and characterization of one band of unidentified material revealed the presence of an additional nucleoside. On the basis of chromatographic and electrophoretic behavior and UV-absorption spectra, it was tentatively identified as 5-hydroxymethyluridine.
D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D004586:Electrophoresis; D006868:Hydrolysis; D012335:RNA, Ribosomal; D013056:Spectrophotometry, Ultraviolet; D014529:Uridine / Q000031:analogs & derivatives*
D012335:RNA, Ribosomal; C012367:5-hydroxymethyluridine; C010194:5-hydroxyuridine; D014529:Uridine