Some observations on the determination of cortisol in human plasma by radioimmunoassay using antisera against cortisol-3-BSA.
Antisera were obtained from rabbits immunised against cortisol-3-BSA with a view to examining their application in a radioimmunoassay of the steroid. One such antiserum was studied in detail; cross-reactivity with other C21 steroids normally present in human plasma was negligible and it proved possible to establish a radioimmunoassay which satisfied all criteria of reliability. The specificity of the cortisol determination achieved in human plasma was examined by performing measurements with and without including an initial Sephadex LH-20 column chromatographic purification step; the values obtained were in excellent agreement both for normal plasma and for that obtained following adrenal stimulation with ACTH.
D000224:Addison Disease / Q000097:blood; D000328:Adult; D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D006801:Humans; D006854:Hydrocortisone / Q000097:blood* / Q000276:immunology; D008722:Methods; D011485:Protein Binding; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine
D012710:Serum Albumin, Bovine; D006854:Hydrocortisone