Isolation and partial characterization of human kidney gangliosides.
1. Eight gangliosides were purified from chloroform/methanol extracts of human kidneys by using modified Folch partition, dialysis, ethanol precipitation, silicic acid column chromatography and preparative thin-layer chromatography. 2. By thin-layer chromatographic behaviour and gas-liquid chromatographic determinations the main gangliosides in human kidney are N-acetylneuraminyllactosylceramide (74% of total) and di-N-acetylneuraminyllactosylceramide (19% of total). 3. Five hexosamine-containing fractions were isolated. Four of them were homogeneous on thin-layer chromatography, and one contained two gangliosides. By gas-liquid chromatography-mass spectrometry it was shown that two gangliosides (together 5% of total) contain glucosamine, and one (1% of total) contains galactosamine. The other of the glucosamine gangliosides contains fucose in addition to the usual sugars found in gangliosides. Of the two remaining hexosamine positive fractions (together 1% of total) one was homogeneous on thin-layer chromatography, the other contained two gangliosides. These two fractions contained both glucosamine and galactosamine. 4. The main long-chain base in all fractions was sphingosine.
D002849:Chromatography, Gas; D005688:Galactosamine / Q000032:analysis; D005732:Gangliosides / Q000302:isolation & purification; D005944:Glucosamine / Q000032:analysis; D006801:Humans; D007668:Kidney / Q000032:analysis*; D013058:Mass Spectrometry; D012794:Sialic Acids / Q000032:analysis; D013110:Sphingosine / Q000032:analysis
D005732:Gangliosides; D012794:Sialic Acids; D005688:Galactosamine; D005944:Glucosamine; D013110:Sphingosine