[Demonstration of isoenzymes of human serum N-acetyl-beta-D-hexosaminidases, during pregnancy].
The N-acetyl-beta-D-hexosaminidase activity (EC 3.2.1.30) in human serum during pregnancy is increased. A polyacrylamide gel electrophoresis, without sodium dodecyl sulfate-uree, of the total serum allowed after incubation with the N-acetyl-beta-D-glucosaminide (6-bromo-2-hydroxy-3-naphthoyl-O-anisidine) coupled with the diazonium salt of O-amino-azotoluene to localise two zones of enzymatic activity. An electrophoresis of the same samples in exponential gradient polyacrylamide gel, shows 6 to 8 zones of different enzymatic activities.
D000118:Acetylglucosaminidase / Q000097:blood*; D000578:Amidines / Q000378:metabolism; D000580:Amido Black; D000606:Amino Sugars / Q000378:metabolism; D001797:Blood Protein Electrophoresis; D003224:Congo Red; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D006596:Hexosaminidases / Q000097:blood*; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D011247:Pregnancy
D000578:Amidines; D000606:Amino Sugars; D007527:Isoenzymes; D003224:Congo Red; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D000580:Amido Black
Mise en évidence d'isoenzymes des N-acétyl-beta-D-hexosaminidases du sérum humain pendant la gestation