Pyrogen from mouse macrophages causes fever in mice.
Mouse peritoneal macrophages, after phagocytosis, release an endogenous (leucocyte) pyrogen. Intravenous injection of stimulated cell culture supernatant produces a prompt, monophasic fever in mice maintained in a 35 degree environment. The pyrogen is distinct from endotoxin, and resembles cell pyrogens of other species in heat-lability and pronase sensitivity. Human leucocyte pyrogen produces identical responses in mice. Measurement of fever in mice appears to provide a sensitive biological assay for endogenous pyrogens.
D000818:Animals; D001681:Biological Assay; D001831:Body Temperature; D001833:Body Temperature Regulation; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D005334:Fever / Q000209:etiology*; D008264:Macrophages / Q000378:metabolism* / Q000502:physiology; D051379:Mice / Q000502:physiology*; D010587:Phagocytosis; D011749:Pyrogens / Q000032:analysis
D004731:Endotoxins; D011749:Pyrogens