Lipid metabolism of developing central nervous tissues in organotypic cultures. II. A comparative study of medium and tissue fatty acids in developing rat cerebral cortex and cerebellum.
Organotypic cultures of rat cerebral cortex and cerebellum have been supplemented with different media. Fatty acid analysis in incubated media and in tissue explants revealed a characteristic differentiation of three periods during maturation: a posttraumatic repair phase, a synaptogenic period and a synaptic stage. Medium fatty acids are biphysically depleted from the nutrient. Both tissues differ in the depletion pattern of individual fatty acids. The development of tissue fatty acids is mainly observed during the synaptogenic period. Turnover studies of individual fatty acids indicate a tissue-specific and time-dependent characteristic.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002531:Cerebellum / Q000196:embryology / Q000378:metabolism*; D002540:Cerebral Cortex / Q000196:embryology / Q000378:metabolism*; D003470:Culture Media; D046508:Culture Techniques; D005227:Fatty Acids / Q000378:metabolism*; D008042:Linolenic Acids / Q000378:metabolism; D050356:Lipid Metabolism; D009829:Oleic Acids / Q000378:metabolism; D010168:Palmitates / Q000378:metabolism; D051381:Rats; D013229:Stearic Acids / Q000378:metabolism
D001095:Arachidonic Acids; D003470:Culture Media; D005227:Fatty Acids; D008042:Linolenic Acids; D009829:Oleic Acids; D010168:Palmitates; D013229:Stearic Acids