[Modification of the indirect hemagglutination test for rapid detection and identification of arbovirus antigens in brain suspensions from infected suckling mice].
It has been found possible to use erythrocytes sensitized by antibody from immune ascitic fluids of white mice for detection and identification of arbovirus antigens in suspensions of brains from infected suckling mice. Nonspecific agglutination of sensitized erythrocytes by animal brain suspensions could be eliminated considerably by increasing NaCl concentration in the reaction ingredient mixture pu to 1.3 M. Specific hemagglutination by the virus antigens present in the brain suspensions was retained. The diagnostic titer of the test was 1:8. Preliminary addition of homologous immune globulin to the antigenic material specifically inhibited agglutination of sensitized erythrocytes.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D001103:Arboviruses / Q000276:immunology*; D001202:Ascitic Fluid / Q000276:immunology; D001921:Brain / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests / Q000379:methods*; D051379:Mice / Q000276:immunology; D012965:Sodium Chloride
D000914:Antibodies, Viral; D000956:Antigens, Viral; D012965:Sodium Chloride
Modifikatsiia reaktsii nepriamoi gemaggliutinatsii dlia bystrogo vyiavleniia i identifikatsii arbovirusnykh antigenov v suspensiiakh mozga infitsirovannykh myshei-sosunkov