Further study on the two-column plasma catecholamine assay.
The two-column procedure for plasma catecholamine determination was adopted as a routine diagnostic test. It was found that the recovery of catecholamine added to properly preserved plasma was 65%. The most efficient recovery occurred when the water content of alumina used was above 23%. Both interassay and intra-assay precision were +/- 10% (coefficient of variation). The catecholamine was stable, in properly preserved plasma, for at least 18 days at -20 degrees C.
D000535:Aluminum; D002395:Catecholamines / Q000097:blood* / Q000302:isolation & purification; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004837:Epinephrine / Q000097:blood; D005470:Fluorometry / Q000379:methods; D006801:Humans; D007475:Ion Exchange Resins; D009638:Norepinephrine / Q000097:blood; D010087:Oxides; D014867:Water / Q000032:analysis
D002395:Catecholamines; D007475:Ion Exchange Resins; D010087:Oxides; D014867:Water; D000535:Aluminum; D009638:Norepinephrine; D004837:Epinephrine