Nonpolar lipids of a halotolerant species of Staphylococcus epidermidis.
The nonpolar lipids of a halotolerant Staphylococcus epidermidis, isolated in pure culture from a growth medium for extreme halophiles containing 25% sodium chloride, were found to contain squalene, menaquinone-7, free fatty acids (mainly anteiso-15:0 and anteiso-17:0), undecaprenol, nonaprenol with predominately cis-isoprene residues, heptaprenol, with predominately trans-isoprene residues, and 1,2- and 1,3-diglycerides containing anteiso- 15:0 and anteiso-17:0 branched chain fatty acid residues. The above compounds were isolated in pure form by column and thin-layer chromatography and were characterized by ultraviolet, proton magnetic resonance, and mass spectra. Fatty acid moieities were characterized by gas-liquid chromatographic retention times of their methyl esters.
D002855:Chromatography, Thin Layer; D004075:Diglycerides / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005233:Fatty Alcohols / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D013058:Mass Spectrometry; D013185:Squalene / Q000032:analysis; D013210:Staphylococcus / Q000032:analysis*
D004075:Diglycerides; D005230:Fatty Acids, Nonesterified; D005233:Fatty Alcohols; D008055:Lipids; D013185:Squalene