Electrophoretic properties of sodium dodecyl sulfate and related changes in its concentration in SDS-polyacrylamide gel electrophoresis.
Sodium dodecyl sulfate(SDS) in a protein sample solution migrates in SDS-polyacrylamide gel electrophoresis as a band with a mobility higher than those of protein bands. Behind this band, which is mostly composed of SDS micelles, SDS concentration is raised uniformly in a gel column as a result of the retardation effect of the gel matrix on SDS micelles. Electrophoretic patterns of SDS were obtained when SDS was omitted from various portions of the gel electrophoretic system.
D001665:Binding Sites; D004566:Electrodes; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007202:Indicators and Reagents; D008823:Micelles; D008970:Molecular Weight; D011506:Proteins; D012967:Sodium Dodecyl Sulfate
D007202:Indicators and Reagents; D008823:Micelles; D011506:Proteins; D012967:Sodium Dodecyl Sulfate