title: Effects of selected sulfhydryl inhibitors on nonhistone chromosomal proteins of HeLa cells.
abstract: Effects of the SH inhibitor sodium iodoacetate, alone and with adjuncts menadiol diphosphate, sodium malonate, sodium fluoride and heparin, on incorporation of tryptophane-3 H into nonhistone chromosomal proteins of HeLa cells were examined. The drugs block incorporation of tryptophane-3 H into nonhistone chromosomal proteins far more than incorporation of leucine-3 H into total cellular proteins. Drug effects on thymidine phosphorylation and DNA synthesis in HeLa cells exceed corresponding effects on fibroblasts from normal healing wounds.
mesh_terms: D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005347:Fibroblasts; D005459:Fluorides / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects* / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008314:Malonates / Q000494:pharmacology; D008466:Mechlorethamine / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009698:Nucleoproteins / Q000096:biosynthesis*; D014364:Tryptophan / Q000378:metabolism; D014812:Vitamin K / Q000494:pharmacology
chemical_list: D004273:DNA, Neoplasm; D007461:Iodoacetates; D008314:Malonates; D009363:Neoplasm Proteins; D009698:Nucleoproteins; D014812:Vitamin K; D008466:Mechlorethamine; D014364:Tryptophan; D006493:Heparin; D004254:DNA Nucleotidyltransferases; D007930:Leucine; D005459:Fluorides