Gas-liquid chromatographic determination of nikethamide in injectable preparations.
A gas-liquid chromatographic (GLC) method, using a 4% XE-60 on 80-100 mesh Gas-Chrom Q column, a flame ionization detector, and anthracene as the internal standard, has been developed for the direct determination of nikethamide. Eight collaborators analyzed 4 samples, using methanol as the solvent; the coefficients of variation obtained ranged from 1.19 to 3.20%. In a limited study with acetone as the solvent, the coefficients of variation ranged from 0.59 to 1.96%. The GLC method with acetone as a solvent has been adopted as official first action.
D002849:Chromatography, Gas / Q000379:methods*; D008832:Microchemistry; D009552:Nikethamide / Q000032:analysis*; D012996:Solutions / Q000032:analysis; D012997:Solvents
D012996:Solutions; D012997:Solvents; D009552:Nikethamide