Translation of Rauscher  leukemia virus RNA in heterologous cell-free systems.
Rauscher leukemia virus RNA (RLV RNA) is translated in mammalian cell-free systems into distinct polypeptides which are immunoprecipitable by an antiserum directed against RLV proteins. These polypeptides partially comigrate electrophoretically with native viral proteins synthesized in vivo in JLS-V9 cells. Besides 72000-, 65000- and 50000-dalton polypeptides a 15000-dalton polypeptide is also synthesized in vitro. Analysis of incubations of RLV RNA in different cell-free systems reveals that no virus-specific factors are required in the translation of RLV RNA in vitro.
D000818:Animals; D002287:Carcinoma, Krebs 2 / Q000378:metabolism; D002460:Cell Line; D002474:Cell-Free System; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism*; D011817:Rabbits; D011925:Rauscher Virus / Q000378:metabolism*; D012156:Reticulocytes / Q000378:metabolism
D012367:RNA, Viral