Effect of cycloheximide on viral precursor protein B in Sindbis virus-infected BHK cells.
The possible role of B protein, a postulated precursor of viral envelope proteins was studied in Sindbis virus-infected BHK cells. Although tryptic digestion of the B protein produces tryptic peptides of the viral envelope proteins it seems unlikely that B molecules accumulated in the cytoplasm of infected cells are subsequently cleaved into structural proteins. We suggest that if B is really the precursor of E1 and PE2 viral envelope proteins, the cleavage takes place on the ribosomes during the synthesis of the second part of the B protein. Low concentrations of cycloheximide completely blocked the synthesis of B protein but did not inhibit the synthesis of viral structural proteins.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology*; D007668:Kidney; D011498:Protein Precursors / Q000096:biosynthesis*; D012845:Sindbis Virus / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*
D011498:Protein Precursors; D014764:Viral Proteins; D003513:Cycloheximide