[Influence of some cytostatic drugs on semiconservative DNA-synthesis and DNA-repair in normal and cancer cells (author's transl)].
The action of cyclophosphamide, vincristine, vinblastine and procarbacine on the enzymes of the excision repair and on the semiconservative DNA-synthesis was investigated. To get a view of the total repair enzyme complexes the incorporation of 3H-thymidine into DNA after UV- and Gamma-irradiation was measured, and sedimentation profiles in alkaline sucrose were carried out. The drugs were also tested after being metabolized. The 4 drugs had no influence in an in vitro system on DNA-repair using spleen cells. Vinblastine and procarbacine showed an inhibitory effect on semiconservative DNA-synthesis. Using Yoshida ascites cells for the investigations we found an inhibition of the exonuclease-polymerase system by cyclophosphamide and procarbacine and a supression of ligase reaction by vinblastine and vincristine. Different degrees of inhibition of DNA-synthesis in Yoshida ascites cells could be seen after use of the 4 cytostatic drugs.
D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003520:Cyclophosphamide / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005720:Gamma Rays; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D011118:Polynucleotide Ligases / Q000378:metabolism; D011344:Procarbazine / Q000494:pharmacology*; D011830:Radiation Effects; D051381:Rats; D012517:Sarcoma, Yoshida / Q000378:metabolism; D013154:Spleen / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays; D014747:Vinblastine / Q000494:pharmacology*; D014750:Vincristine / Q000494:pharmacology*
D004273:DNA, Neoplasm; D011344:Procarbazine; D014750:Vincristine; D014747:Vinblastine; D003520:Cyclophosphamide; D004247:DNA; D011118:Polynucleotide Ligases
Die Wirkung einiger Zytostatika auf die semikonservative DNS-Synthese und auf die DNS-Reparatur von normalen und Tumorzellen