Heterogeneity among preparations of crude trypsin used to disaggregate the human tonsil.
Crude trypsin is the agent of choice for the  disaggregation of human tonsil. A great variability which is not related to specific activity exists among lots of crude trypsin. An efficient lot of crude trypsin is necessary to obtain optimal proportions of tonsillar cells for the purification of lymphocytes and plasma cells by a previously reported technique.
D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D014066:Palatine Tonsil / Q000166:cytology / Q000187:drug effects*; D014357:Trypsin / Q000032:analysis / Q000494:pharmacology / Q000592:standards
D014357:Trypsin