[Activity of acid phosphatase and succinate and dehydroorotate dehydrogenases during the interaction of allogeneic lymphocytes and target cells in tissue culture].
A study was made of the activity of acid phosphatase in interaction of lymphocytes and target cells; it was shown that in the lymphocytes its greatest increase occurred in the 1- and sometimes in the 3-hour cultures; sometimes the activity of the enzyme in the L-cells increased by the 3rd-6th hour. The initial activity of acid phosphatase was greater in the immune lymphocytes; later, on their addition to the L-cell culture, there was no significant difference in the changes in the activity of the enzyme in the immune and normal lymphocytes. In the L-cells the activity of acid phosphatase was lower on addition of normal lymphocytes in comparison with the immune ones. The activity of dehydrogenases in the lymphocytes increased by the 3rd hour of the incubation period and was greater in the immune lymphocytes than in the normal ones. The activity of the succininc- and dehydroorotic dehydrogenases altered practically at the same periods in the L-cells as in the lymphocytes. The intensification of the activity of the redox enzymes and of the acid phosphatase during the first hours of contact pointed to the rapid activation of the effector lymphocytes.
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D000937:Antigen-Antibody Reactions; D003601:Cytotoxicity Tests, Immunologic; D066298:In Vitro Techniques; D007739:L Cells / Q000201:enzymology / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009963:Orotic Acid; D010088:Oxidoreductases / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism
D009963:Orotic Acid; D010088:Oxidoreductases; C019999:dehydroorotate dehydrogenase; D013385:Succinate Dehydrogenase; D000135:Acid Phosphatase
Izuchenie aktivnosti kisloĭ fosfatazy i degidrogenaz suktsinata i degidroorotata pri vzaimodeĭstvii allogennykh limfotsitov i kletok-misheneĭ kul'ture tkani