Interactions of KB-cell glycoproteins with an adenovirus capsid protein.
Glycoprotein material extracted from human KB cells with a flurocarbon, trichlorotrifluoroethane (Arklone P), into a waste-soluble fraction binds to fibre, a structural protein of the adenovirus type-5 capsid. The fibre-binding glycoprotein(s) were purified by ion-exchange chromatography on DEAE-Sephadex and affinity chromatography on a fibre-Sepharose support. The purification procedure also includes a trypsinization step which eliminates the bulk of contaminating KB cell proteins present in the aqueous fraction without appreciably affecting the activity of the fibre-binding glycoprotein(s). Some comparison is made of the membrane-bound receptors for adenovirus and the water-soluble fibre-binding glycoprotein(s).
D000256:Adenoviridae; D001665:Binding Sites; D002460:Cell Line; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000302:isolation & purification; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification; D011485:Protein Binding; D014764:Viral Proteins / Q000302:isolation & purification
D006023:Glycoproteins; D009363:Neoplasm Proteins; D014764:Viral Proteins