Effect of dietary rapeseed oil on hepatic hexobarbital metabolism in mice.
The effect of dietary rapeseed oil (RSO) on hepatic hexobarbital metabolism has been studied in short-term experiments in mice by measuring both the activity of mixed function oxidase system in microsomal preparations in vitro and the in vivo hexobarbital-induced sleeping time. Moreover, the fatty acid pattern of liver tissue, with particular regard to erucic and gadoleic acids, was analyzed gas-chromatographically. The hexobarbital metabolism in young RSO-fed (RSO containing 52% of erucic acid and 7.4% of gadoleic acid; 50 cal% for 3 days) is lower by about 44% than in controls treated in the same way with peanut oil, independently of sex. RSO did not have a statistically significant effect of hexobarbital metabolism in adult animals of both sexes. As to the dose-response study, the prolongation of the hexobarbital-induced sleeping time in young male is statistically significant when fed with 30 cal% in RSO for 3 days, while it is not more statistically significant in mice fed 15 cal% in RSO. In RSO mice (50 cal%) erucic and gadoleic acid account both for about 10% of the total fatty acids in the liver tissue, while they are quite absent in controls. It causes a relative deficiency of linoleic and arachidonic acids, known to be essential for synthesis and maintenance of the membranes supporting hepatic microsomal enzymes responsible for the metabolism of some drugs.
D000818:Animals; D010367:Arachis; D001711:Biotransformation; D004041:Dietary Fats; D005227:Fatty Acids / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005260:Female; D006591:Hexobarbital / Q000378:metabolism*; D008658:Inactivation, Metabolic; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology; D009821:Oils / Q000494:pharmacology*; D010088:Oxidoreductases / Q000378:metabolism*; D012639:Seeds; D012890:Sleep / Q000187:drug effects; D013997:Time Factors
D004041:Dietary Fats; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D009821:Oils; D006591:Hexobarbital; D010088:Oxidoreductases