The effect of intact and lysed leukocytes on murine tumor growth and host survival.
The inhibitory effect of sensitized lymph-node cells on transplanted tumor growth in the mouse is well documented. The efficacy of peripheral lymphocytes is disputed. Lysed lymphocyte extracts ("transfer factor"), although effective in transferring several types of delayed hypersensitivity in man, have had equivocal results in animals, particularly in tumor immunity. Fifty 129SV mice were sensitized over 1 month with multiple injections of a transplantable tumor that had arisen in the strain as a spontaneous testicular teratoma. These lymphocyte donor mice were sacrificed and lymphocytes pooled. Half of the lymphocytes were given intact to the 25 mice of experimental group A. Remaining lymphocytes were lysed and given to the 25 mice of group B. The control group C of 25 mice received intact lymphocytes from unsensitized donors. All mice were then challenged with 10(6) tumor cells subcutaneously. Two weeks after challenge no animals in the treated groups (A and B) had palpable tumors, whereas 32% in the control group C had palpable tumors. After 3 weeks 25% of group A, 42% of group B, and 63% of group C had tumors. Tumors ultimately grew in 60% of group A, 76% of group B, and 88% of group C. The effect of treatment on rate of tumor growth after appearance was variable, showing inhibition in some and enhancement in others. The ability of intact, sensitized lymphocytes and lymphocyte extracts to confer relative tumor immunity was demonstrated.
D000818:Animals; D005260:Female; D007106:Immune Sera / Q000008:administration & dosage; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental; D013724:Teratoma / Q000276:immunology* / Q000401:mortality / Q000473:pathology; D013736:Testicular Neoplasms / Q000276:immunology* / Q000401:mortality / Q000473:pathology
D007106:Immune Sera