[Ultrastructural study of the pseudopregnant rat luteal cell following the effect of luteinizing hormone and of 2 inhibitors of synthesis (amino-glutethimide and cycloheximide)].
Incubation of ovaries from pseudopregnant rats have been performed with the gonadotropin LH and with two inhibitors, aminoglutéthimide and cycloheximide for periods comprised between 1 and 6 hours. Then the luteal cells have been studied with electron microscope. Measurements in have been used to express the variations in volume of two organels, mitochondria and lipid droplets. The principal modifications induced by these compounds bear on the mitochondria. Treatment with LH is followed by an increase in the volume of individual mitochondria resulting probably of fusion of organels. Treatments with the inhibitors are followed by reduction in the volume of individual mitochondria and in the total volume of mitochondrial apparatus. Mixed treatments using LH and an inhibitor allow to obtain organels of intermediate size. Volume of lipids is increased by incubation with LH and probably decreased by the inhibitors. Smooth endoplasmic reticulum shows rearrangements with the various treatments. It seems not convenient to extend incubations over 4 hours in order to avoid cytolysis.
D000616:Aminoglutethimide / Q000494:pharmacology*; D000818:Animals; D003338:Corpus Luteum / Q000648:ultrastructure*; D003513:Cycloheximide / Q000494:pharmacology*; D005260:Female; D008184:Luteal Cells / Q000648:ultrastructure*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects; D008933:Mitochondrial Swelling / Q000187:drug effects; D011374:Progesterone / Q000096:biosynthesis; D011555:Pseudopregnancy / Q000187:drug effects; D051381:Rats
D000616:Aminoglutethimide; D011374:Progesterone; D007986:Luteinizing Hormone; D003513:Cycloheximide
Etude ultrastructurale de la cellule lutéale de la ratte pseudogestante après action de l'hormone lutéinisante et de deux inhibiteurs de synthèse (aminoglutéthimide et cycloheximide