Two rapid and improved techniques for chromatographic fractionation of casein.
The major components of casein, alphas-, beta-, kappa-, and gamma-caseins, were chromatographically fractionated on diethylaminoethyl ion-exchange cellulose in stepwise elution with pH 8, .03 M glycine, or pH 7, .005 M phosphate buffers in the presence of 3.3 M urea and .1% 2-mercaptoethanol. Fractionation was at room temperature (around 25 C) in 4 to 4.5 h. The components showed a reasonable purity electrophoretically. These techniques can be used for quantitative estimation of casein composition.
D002364:Caseins / Q000032:analysis*; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D004592:Electrophoresis, Starch Gel
D002364:Caseins