Selective solubilization of hemagglutinin and neuraminidase from influenza viruses.
A novel approach is described for the isolation of hemagglutinin and neuraminidase from influenza viruses. In contrast to published procedures, the two glycoproteins are selectively solubilized, leaving an intact viral subparticle which contains lipid, RNA and non-glycoproteins. The pronounced differences in size allow a simple separation of the solubilized proteins from the virus 'core'.
D002593:Cetrimonium Compounds; D006023:Glycoproteins / Q000302:isolation & purification*; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D007252:Influenza Vaccines; D008055:Lipids / Q000032:analysis; D009439:Neuraminidase / Q000302:isolation & purification*; D009975:Orthomyxoviridae / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D012367:RNA, Viral / Q000032:analysis; D012995:Solubility; D013501:Surface-Active Agents; D014764:Viral Proteins / Q000032:analysis / Q000302:isolation & purification*
D002593:Cetrimonium Compounds; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D008055:Lipids; D012367:RNA, Viral; D013501:Surface-Active Agents; D014764:Viral Proteins; D009439:Neuraminidase