[Point of micropreparative high pressure liquid chromatography and thin-layer chromatography for the identification of indole compounds in human plasma (author's transl)].
Results are presented on the analysis of indolic metabolites of tryptophan in human plasma, using high-pressure liquid chromatography and thin-layer chromatography. Dichloromethane/ethanol extracts of denaturated plasma were analysed. Thin-layer chromatography proved to be more advantageous for the analysis of this class compounds because it is possible to use a specific staining reagent (4-dimethylaminobenzaldehyde). The advantage of high-pressure liquid chromatography lies in the rapid isolation and purification of unknown compounds for identification in an off-line method. This application is demonstrated with the isolation of N-acetyltryptophan from human plasma. Preliminary results are presented on the plasma concentration of indole-3-lactic acid, indole-3-acetic acid and N-acetyltryptophan in healthy persons, phenylketonurics, and uremic patients.
D000293:Adolescent; D000328:Adult; D001547:Benzaldehydes; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005260:Female; D006801:Humans; D007210:Indoleacetic Acids / Q000097:blood; D007211:Indoles / Q000097:blood*; D007773:Lactates / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D010661:Phenylketonurias / Q000097:blood; D014364:Tryptophan / Q000031:analogs & derivatives / Q000097:blood; D014511:Uremia / Q000097:blood
D001547:Benzaldehydes; D007210:Indoleacetic Acids; D007211:Indoles; D007773:Lactates; D014364:Tryptophan
Kombinierte Anwendung der mikropräparativen Hochdruck-Flüssigkeitschromatographie und der Dünnschichtchromatographie beim Nachweis von Indolmetaboliten im menschlichen Plasma