title: [Antigenic structure of a fragment of human serum albumin].
abstract: The antigenic structure of a fragment (F1) of human serum albumin (HSA), carrying one of the antigenic sites of the whole molecule, has been studied. Previous studies have shown that this fragment is made of two peptide chains linked by a disulfide bond. One of these chains named alpha has 28 residues and one intrachain disulfide bond giving rise to a loop of 9 or 10 residues. The chain occurs under two forms: the beta chain made of 25 residues and the gamma chain which differs from the beta chain by the absence of the two last C-terminal residues (F. Bellon and C. Lapresle, Biochem. J., 1975, 147, 585-592). The alpha and beta chains both inhibit independantly at 100% the agglutination by an anti-albumin serum of red cells sensitized with F1. Chains alpha, beta and gamma displace anti-HSA antibodies fixed upon an immunoadsorbent prepared with F1. Chain alpha is more active than chain beta which in turn is more active than chain gamma. Reduction and alkylation of alpha and beta chains abolish almost completely their immunological activity. Immunoadsorbents prepared with alpha or beta chains adsorb all of the anti-F1 antibodies from an anti-HSA serum. Antibodies isolated from each chain are inhibited by both chains. Chains alpha and beta induce in rabbits the formation of antibodies reacting with F1 and HSA, alpha chain being a better immunogen than beta chain.
mesh_terms: D062365:Aminobenzoates / Q000378:metabolism; D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D007457:Iodine Radioisotopes; D012709:Serum Albumin / Q000032:analysis / Q000276:immunology*
chemical_list: D062365:Aminobenzoates; D000906:Antibodies; D000941:Antigens; D007457:Iodine Radioisotopes; D012709:Serum Albumin
vernacular_title: Etude de la structure antigénique d'un fragment de sérum albumine humaine