[Morphologic and biochemical heterogeneity of lysosomes].
By means of isopycnic centrifugation in the continuous density gradient of sucrose two subfractions of lysosomes were isolated from rat liver homogenates: a "light" one (with the floating density p=1.13) and a "heavy" one (p=1.24). Electron microscopic, enzymatic and electron microscope enzymatic analysis of the isolated subfractions showed that the "light" subfraction consisted mainly of newly-formed primary lysosomes, while the "heavy" one was presented by secondary lysosomes. Parallel biochemical investigations demonstrated a considerable enzymatic heterogeneity of the two lysosomal subfractions: the "light" subfraction was characterized by a high specific activity of acid DNase, acid RNase and beta-galactosidase, and by almost total absence of beta-glucosidase activity, while the "heavy" one was characterized by a high specific activity of beta-glucosidase, beta-glucuronidase and beta-N-acetylglucosaminidase. Possible causes of enzyme heterogeneity of rat liver lysosomes are discussed.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D005696:Galactosidases / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure*; D008247:Lysosomes / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism
D003851:Deoxyribonucleases; D012260:Ribonucleases; D001192:Arylsulfatases; D005696:Galactosidases; D005959:Glucosidases; D005966:Glucuronidase; D000118:Acetylglucosaminidase
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