A simple rapid separation of C1-esterase using an immunoadsorbent column.
An immunoadsorbent-antibody (egg albumin-Sepharose-antibody) column was found to be suitable for the rapid separation of C1-esterase from normal human serum. About 1.54 mg of C1-esterase, with a specific activity of 447 units/mg was obtained in voer 80% yield from the 20 ml of human serum.
D000327:Adsorption; D000418:Albumins; D000906:Antibodies; D002846:Chromatography, Affinity; D003165:Complement System Proteins / Q000302:isolation & purification*; D004527:Egg Proteins; D004950:Esterases / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D012685:Sepharose
D000418:Albumins; D000906:Antibodies; D004527:Egg Proteins; D003165:Complement System Proteins; D012685:Sepharose; D004950:Esterases