A method for detecting factor-VIII clotting activity associated with factor VIII-related antigen in agarose gels.
A method for detecting factor-VIII clotting activity in agarose is described. It is based on factor VIII promoting coagulation in a mixture of haemophilic plasma in agarose which is detected by a change in opacity. When this test was used to detect factor-VIII clotting activity in a one-dimensional Laurell electroimmunoassay for factor VIII-related antigen all the factor-VIII activity was found in the same position as the factor VIII-related antigen immunoprecipitate. Factor-VIII clotting activity did not appear to be simply trapped in this immunoprecipitate and therefore it has been concluded that the molecule containing factor-VIII clotting activity carries factor VIII-related antigen determinants.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001666:Binding Sites, Antibody; D001777:Blood Coagulation; D005169:Factor VIII / Q000032:analysis / Q000276:immunology; D006467:Hemophilia A / Q000097:blood / Q000276:immunology; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D011817:Rabbits / Q000276:immunology; D012685:Sepharose
D000941:Antigens; D005169:Factor VIII; D012685:Sepharose