[Studies on tissue cultures from calf eyes].
The growth of lens epithelium and iris tissue was studied by biomicroscopy and electronmicroscopy, its velocity measured. With the Warburg-method it was possible to show the oxygen-uptake of both tissues without and after addition of different substances. Pilocarpine lowered the O2-uptake of the epithelia to less than 1/3 of its normal value, whilst it raised that of the iris to the double amount. Napthochinone inhibited the O2-uptake of both tissues to little more than half of the normal level. The least differences as compared to the controls showed methyl-prednisolone: it lowered scarcely the O2-uptake of the epithelium, and the O2-uptake of the iris to almost half the normal rate. The author concludes that cataract developes, if oxygen-uptake of lens epithelium and iris tissue are reduced in the same way without any possibility of compensation. Under certain conditions this cell-biological method may be employed to detect cataractogenous substances.
D000818:Animals; D002417:Cattle; D046508:Culture Techniques; D005123:Eye / Q000648:ultrastructure*; D007498:Iris / Q000378:metabolism / Q000648:ultrastructure; D007908:Lens, Crystalline / Q000378:metabolism / Q000648:ultrastructure; D008775:Methylprednisolone / Q000494:pharmacology; D008854:Microscopy, Electron; D010101:Oxygen Consumption / Q000187:drug effects; D010862:Pilocarpine / Q000494:pharmacology
D010862:Pilocarpine; D008775:Methylprednisolone
Untersuchungen an Gewebekulturen aus Kälberaugen