Purification and properties of an esterase B4 from human liver.
A butyryl esterase, designated B4, has been purified from human liver and some of its properties described. The activity of this enzyme comprises 0.48% of the total butyryl esterase activity found in human liver. Esterase B4 has been distinguished from other butyryl esterases by its preference for the esters of the fluorogenic compounds 4-methyl umbilliferone and fluorescein over naphthyl esters as substrates. Other distinguishing features of this esterase include a relatively high pI (pH 8.7) A monomeric structure of low molecular weight (20 000) and high solubility in solutions of ammonium sulphate.
D000596:Amino Acids / Q000032:analysis; D004950:Esterases / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007525:Isoelectric Focusing; D008099:Liver / Q000201:enzymology*; D010971:Plastoquinone / Q000031:analogs & derivatives*; D011809:Quinones / Q000031:analogs & derivatives*
D000596:Amino Acids; D011809:Quinones; D004950:Esterases; D010971:Plastoquinone