Primary culture and maintenance of steroid-secreting human placenatal monolayers.
A simple method is described for primary culture and for maintenance of hormone-producing cells from normal human placenta. A consistent yield of cells was obtained and an average survival of 3 to 4 months in culture using 1 mm3 explants from the most vascular area of the placentas. These explants were placed in a variety of culture media in 30 ml flasks and incubated at 37 degrees C in an atmosphere of 5% CO2 and 95% air. The best yields in terms of cell growth were observed with Eagle's MEM (minimum essential medium) with supplements of horse serum and fetal calf serum or human cord serum. (Ham's F-10 with supplement of horse serum and fetal calf serum supports growth for the longest period and media containing human cord serum had the best yield of steroids.
D002470:Cell Survival; D002478:Cells, Cultured / Q000378:metabolism; D003470:Culture Media; D005260:Female; D006801:Humans; D010920:Placenta / Q000166:cytology*; D010926:Placental Hormones / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism
D003470:Culture Media; D010926:Placental Hormones; D011374:Progesterone