Intraction of aminoacyl-tRNA synthetases with ribosomes and ribosomal subunits.
Salt-washed ribosomes from rabbit reticulocytes stimulate seven partially purified aminoacyl-tRNA synthetases up to threefold: arginyl-, alanyl-, isoleucyl-, lysyl-, methionyl-, phenylalanyl- and valyl-tRNA synthetase. Both isolated subunits but not total ribosomal RNA show the stimulation. The increase in synthetase activity is also found in a highly purified Escherichia coli system employing homogenous (E. coli)phenyl-alanyl-tRNA synthetase and (E. coli)- tRNAPhe. The biological significance of the stimulation is discussed.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002458:Cell Fractionation; D008274:Magnesium / Q000494:pharmacology; D011485:Protein Binding; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014633:Valine
D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D014633:Valine; D008274:Magnesium