Isolation of cold-sensitive Chinese hamster cells.
Six cold-sensitive variants have been isolated from Chinese hamster ovary cells by the BUdR-visible light selection technique. The properties of one of these lines have been studied in detail. This line stops dividing immediately after a shift from 39 degrees C to 33 degrees C though its doubling time at 39 degrees C is only slightly longer than that of wild-type cells. The rates of DNA and protein synthesis are severely reduced at 33 degrees C, but the rate of RNA synthesis is not significantly different from wild-type cells. This line may be defective in protein synthesis, but the results of sedimentation analysis indicate that it probably has normal ribosomal subunit assembly.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003080:Cold Temperature; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D005260:Female; D009154:Mutation; D010053:Ovary; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012270:Ribosomes / Q000032:analysis
D012313:RNA; D004247:DNA; D001973:Bromodeoxyuridine