Inhibition of protein synthesis in mouse myeloma cells by Ricinus communis toxin.
The mechanism of inhibition of protein synthesis in mouse myeloma cells by Ricinus communis toxin was studied. No significant disaggregation of polysomes into monosomes was detected in the toxin-treated cells. The activity of the polysomes isolated from the cells treated with the toxin in protein synthesis was remarkably lower than that of the untreated cells, while the activity of the supernatant enzyme fraction was retained. The ribosomes derived from the polysomes of the toxin-treated cells were inactive in poly(U)-dependent polyphenylalanine synthesis. The activity of ribosomes reconstituted by hybridizing subunits derived from the ribosomes of normal and toxin-treated cells were measured in poly(U)-dependent polyphenylalanine synthesis, and the 60 S subunit was revealed to be inactive. These results indicate that the target of action of the toxin towards intact cells is the 60 S ribosomal subunit.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D051379:Mice; D009101:Multiple Myeloma / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009374:Neoplasms, Experimental / Q000378:metabolism; D010944:Plants; D011072:Poly U / Q000378:metabolism; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D014118:Toxins, Biological / Q000276:immunology / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
D000596:Amino Acids; D009363:Neoplasm Proteins; D014118:Toxins, Biological; D011072:Poly U; D012260:Ribonucleases