[Effect of cytostatic drugs on the metabolism of incubated human blood platelets].
Degranol was chosen from the group of cytostatic drugs and Cytosar and Flucrouracil from the group of antimetabolites for investigations on the effects of cytostatics on the metabolism of thrombocytes. After isolation from blood collected on ACD fluid thromtocytes were suspended in own plasma containing EDTA and the cytostatic agent in a concentration of 6 x 10(-3)M and were subjected to incubation during 3 hours at 37 degrees C. After incubation the thrombocyte count, the levels of nucleotide compounds, and adenine nucleotides (ATP + ADP) released by thrombocytes under the action of thrombin, and the activity of dehydrogenase glucose-6-phosphate and lactic dehydrogenase were determined. The results of these determinations showed that only Degranol as a compounds belonging to the group of alkylating cytostatics caused lowering of ATP level (by about 30%) in the incubated cells. The drugs from the group of antimetabolites caused no significant changes in the studied parameters.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001792:Blood Platelets / Q000201:enzymology / Q000378:metabolism*; D003561:Cytarabine / Q000494:pharmacology*; D005472:Fluorouracil / Q000494:pharmacology*; D006156:Guanosine Diphosphate Sugars / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008357:Mannomustine / Q000494:pharmacology*; D013997:Time Factors
D006156:Guanosine Diphosphate Sugars; D003561:Cytarabine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D008357:Mannomustine; D007770:L-Lactate Dehydrogenase; D005472:Fluorouracil
Badania nad wpływem leków cytostatycznych na metabolizm inkubowanych krwinek płytkowych człowieka