[Simplification of the dinitrophenylhydrazine method for the photometric determination of ascorbic acid and dehydroascorbic acid in fruit juices. I. Application to juice of black current (author's transl)].
The photometric determination of ascorbic acid and dehydroascorbic acid by preparing the 2,4-dinitrophenylhydrazone derivative of dehydroascorbic acid and purifying it by thinlayerchromatography was simplified and adapted to the microliter system of Eppendorf Gerätebau, Hamburg, Germany. Using this method with juice of black current, quite reproducible results for vitamin C contents were obtained. Compared with the method of the International Federation of Fruit Juice Producers (titration with 2,6-dichlorphenolindophenol) the contents of vitamin C found with nine different commercial juices of black current were lower from 13 to 30%.
D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D003683:Dehydroascorbic Acid / Q000032:analysis*; D005638:Fruit / Q000032:analysis*; D006834:Hydrazines; D008722:Methods; D010783:Photometry
D006834:Hydrazines; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid
Vereinfachung der Dinitrophenylhydrazin-Methode zur photometrischen Bestimmung von Ascorbinsäure und Dehydroascorbinsäure in Fruchtsäften