Comparison of sulfobromophthalein (BSP) and sulfobromophthalein glutathione (BSP-GSH) disposition under conditions of altered liver function and in the isolated perfused rat liver.
Disposition of sulfobromophthalein (BSP) and sulfobromophthalein glutathione (BSP-GSH) was compared in control, phenobarbital, or alpha-naphthylisothiocyanate-(ANIT)-treated rats, and in the isolated perfused rat liver preparation. After dye administration, BSP-GSH was found to have a more rapid early plasma disappearance rate, a more rapid appearance in the liver, and a greater rate of biliary excretion both in vivo and in the isolated perfused liver, than that of BSP. In considering these observations, it is concluded that hepatic uptake as well as biliary excretion of BSP-GSH is faster than that of BSP. Comparing BSP and phenobarbital, augmentation or reduction in plasma dye concentration, mean plasma half-life (2 to 30 minutes), hepatic dye content, and bile dye concentration, were of the same order of magnitude for both dyes. However, with dye infusion of 3.6 mumoles per kilogram per minute, phenobarbital significantly enhanced the rate of biliary excretion of BSP but not BSP-GSH and ANIT treatment had a greater inhibitory effect on biliary excretion of BSP-GSH than BSP.
D015058:1-Naphthylisothiocyanate / Q000494:pharmacology; D000818:Animals; D001646:Bile / Q000378:metabolism; D005978:Glutathione / Q000008:administration & dosage / Q000378:metabolism*; D006207:Half-Life; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D013448:Sulfobromophthalein / Q000008:administration & dosage / Q000378:metabolism*
D013448:Sulfobromophthalein; D015058:1-Naphthylisothiocyanate; D005978:Glutathione; D010634:Phenobarbital