Radioimmunoassay for plasma betamethasone 17-benzoate.
A sensitive radioimmunoassay for plasma betamethasone 17-benzoate has been developed. The antiserum used was obtained by immunizing rabbits with betamethasone 17-benzoate-21-hemisuccinate-bovine-serum-albumin conjugate. All of the endogenous steroids tested cross reacted less than 0.10%. A standard curve was established with a useful range from 0.05-5 ng. Reliability criteria were satisfactory. Measurement of plasma concentrations of betamethasone 17-benzoate was performed in patients and in rabbits following occlusive dressing of betamethasone 17-benzoate cream and gel base.
D000818:Animals; D001623:Betamethasone / Q000031:analogs & derivatives / Q000097:blood* / Q000276:immunology; D003429:Cross Reactions; D006801:Humans; D006854:Hydrocortisone / Q000097:blood / Q000276:immunology; D008832:Microchemistry; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay
D001623:Betamethasone; D006854:Hydrocortisone