Osmium (VI) complexes of the 3', 5'-dinucleoside monophosphates, ApU and UpA.
The dinucleoside monophosphates, ApU and UpA, react with potassium osmate (VI) and 2,2'-bipyridyl to form the corresponding oxo-osmium (VI) bipyridyl sugar ester in which the osmate group is bonded to the terminal 2',3'-glycol. Osmium (VIII) tetroxide and 2,2'-bipyridyl react with the dinucleosides to form the corresponding oxo-osmium (VI) bipyridyl heterocyclic esters which result from addition of the tetroxide to the 5,6-double bond of the uracil residue. Although capable of transesterification reactions, these heterocyclic esters are exceptionally stable toward exchange reactions in solution. No apparent exchange was observed after 1 month. This reaction thus seems promising for single-site osmium labeling in polynucleotides.
D000227:Adenine Nucleotides; D001665:Binding Sites; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D009841:Oligonucleotides; D009992:Osmium; D014500:Uracil Nucleotides
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D014500:Uracil Nucleotides; D009992:Osmium