Binding of polyriboinosinic-polyribocytidylic acid with cultured cells.
Homogenates prepared from polyriboinosinic-polyribocytidylic acid copolymer [poly(rI) - poly(rC)]-treated cells exhibited antiviral activity in chick embryo, L and rabbit kidney cells. The antiviral activity in the homogenate co-sedimented with cellular membrane material and was shown to be poly(rI) - poly(rC) by a hybridization competition test with immobilized polyribocytidylic acid. The results indicate that poly(rI) - poly(rC) binds firmly to cellular membrane. These studies, however, could not differentiate between specific binding leading to the interferon induction and non-specific binding possibly unrelated to the induction of interferon.
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured / Q000378:metabolism*; D007372:Interferons / Q000096:biosynthesis*; D007739:L Cells / Q000378:metabolism; D004680:Maus Elberfeld virus / Q000378:metabolism; D051379:Mice; D011070:Poly I-C / Q000378:metabolism* / Q000494:pharmacology; D012845:Sindbis Virus / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D014762:Viral Interference
D007372:Interferons; D011070:Poly I-C