title: Proviral DNA of Moloney leukemia virus: purification and visualization.
abstract: Closed-circular proviral DNA of Moloney leukemia virus has been purified from a 10(7) excess of cellular and mitochondrial DNA. The DNA can be visualized in the electron microscope and has the contour length of a molecule with a moleculecular weight of about 5.5 + 10(6). Electron microscopic observation of a hybrid between viral RNA and this circular DNA confirms the viral origin of this molecule.
mesh_terms: D004270:DNA, Circular / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification*; D008854:Microscopy, Electron; D008979:Moloney murine leukemia virus / Q000032:analysis* / Q000254:growth & development; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral; D012260:Ribonucleases
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D012367:RNA, Viral; D012260:Ribonucleases