Phosphorylation of mononucleotides and formation of cytidine 5'-diphosphate-choline and sugar nucleotides by respiration-deficient mutants of yeasts.
Respiration-deficient mutants (Rho-, petite) of Saccharomyces carlsbergensis were obtained by treatment with trypaflavin (euflavine). Dried cells of these mutants phosphorylated mononucleotides to their triphosphates and further formed not only cytidine 5'-diphosphate-choline, but also sugar nucleotides, such as uridine 5'-diphosphate-glucose, guanosine 5'-diphosphate-mannose, etc. The activities were the same or slightly greater than those of the wild strain. These results showed that energy (adenosine 5'-triphosphate) necessary for phosphorylation of mononucleotides was sufficiently supplied by the glycolysis system.
D000167:Acriflavine; D000255:Adenosine Triphosphate / Q000378:metabolism; D002794:Choline / Q000031:analogs & derivatives*; D003566:Cytidine Diphosphate Choline / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D009153:Mutagens; D009154:Mutation; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D009711:Nucleotides / Q000378:metabolism*; D010101:Oxygen Consumption; D012440:Saccharomyces / Q000378:metabolism*
D009153:Mutagens; D009702:Nucleoside Diphosphate Sugars; D009711:Nucleotides; D000167:Acriflavine; D003566:Cytidine Diphosphate Choline; D000255:Adenosine Triphosphate; D005947:Glucose; D002794:Choline