title: [Effect of lyophilization methods on the formation of free radicals, the survivability and makeup of a population of the lincomycin producer].
abstract: It was shown that a short-time exposure of the spores of Act. roseolus 981 to air (oxygen) on their lyophilization by the chamber method resulted in formation of increased numbers of the free radicals in the lyophilized spores suspension and decreased the survival of the spores on their further storage under vacuum. The phenomena were less pronounced when the exposure of the spores to the air was excluded by using the collector method of lyophilization. This testified to the advantages of the latter method as compared to the chamber one. No effect of the lyophilization methods on the population composition of the spontaneous variants of Act. roseolus 981 was observed.
mesh_terms: D002470:Cell Survival; D005609:Free Radicals; D005612:Freeze Drying / Q000379:methods*; D014644:Genetic Variation; D008034:Lincomycin / Q000096:biosynthesis*; D013171:Spores, Bacterial / Q000502:physiology; D013302:Streptomyces / Q000502:physiology*; D013997:Time Factors
chemical_list: D005609:Free Radicals; D008034:Lincomycin
vernacular_title: Vliianie sposobov liofilizatsii na obrazovanie svobodnykh radikalov, vyzhivaemost' i sostav populiatsii produtsenta linkomitsina