Serum parathyroid hormone: a double antibody radioimmunoassay.
A radioimmunoassay for parathyroid hormone (PTH) using a double antibody system is described. Because of the immunolgoical heterogeneity of the hormone in human serum, the standard used has been serum from a patient with parathyroid carcinoma. With the use of the synthetic 34 amino acid N-terminal fragment of PTH, the anti-PTH antiserum was determined to react primarily with the N-terminal end of the molecule. PTH was detectable in the sera of 25% of normal subjects and elevated in 18 of 19 patients with parathyroid adenoma and carcinoma. Serum PTH levels were elevated in 3 of 5 patients with parathyroid hyperplasia.
D000236:Adenoma / Q000097:blood; D000818:Animals; D002277:Carcinoma / Q000097:blood; D002417:Cattle; D002645:Chickens; D006965:Hyperplasia / Q000097:blood; D007457:Iodine Radioisotopes; D010281:Parathyroid Hormone / Q000097:blood*; D010282:Parathyroid Neoplasms / Q000097:blood; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*
D007457:Iodine Radioisotopes; D010281:Parathyroid Hormone