[Biological assay of prostaglandin using the rat uterus].
Isometric contraction of rat uterus with PGF2alpha was more reliable and stable than when a stomach preparation was used. The method of PG bioassay using rat uterus was as follows: ovariectomized rats were given 10 mug of estrone s.c. 48 hr before the decapitation. Uterine strips were suspended in 2 ml of organ bath containing modified Locke-Ringer solution at 25 degrees C, and the isometric contraction was determined. As little as 4 ng/ml of PGF2alpha was determined quantitatively with this method.
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods*; D004970:Estrone / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000032:analysis; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D011460:Prostaglandins F / Q000032:analysis*; D051381:Rats; D013270:Stomach / Q000187:drug effects; D013696:Temperature; D014590:Uterine Contraction / Q000187:drug effects; D014599:Uterus / Q000187:drug effects*
D011460:Prostaglandins F; D004970:Estrone; D001285:Atropine