Cytophilic attachment of ovine IgG2 to autologous polymorphonuclear leucocytes.
Preparations of ovine polymorphs were stained with fluoroscein-conjugated monospecific anti-immunoglobulin sera. Approximately 26% of polymorphs specifically stained with anti-gamma2, 16% with anti-light chain, 24% with anti-Fab and 24% with anti-ovine serum. There was no significant staining of polymorphs with anti-gamma1, anti-mu or anti-alpha. Cytophilic IgG2 could be removed from the cells by incubation at 37 degress and subsequently replaced by incubation in autologous serum at 4 degrees.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular; D007074:Immunoglobulin G; D007962:Leukocytes / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology*
D007074:Immunoglobulin G