Temperature-sensitive mutants isolated from hamster and canine cell lines persistently infected with Newcastle disease virus.
Evidence is presented which confirms that temperature-sensitive (ts) mutants with an RNA- phenotype are spontaneously selected in persistent infection of cell lines with Newcastle disease virus. Persistently infected BHK-21 cells, maintained since 1973, produce no interferon and are completely susceptible to vesicular stomatitis virus. Persistent infection of a canine kidney cell line (MDCK) terminated with destruction of all cells at about 100 days. Even under these conditions, a high proportion (33%) of RNA- temperature-sensitive mutants was present in the virus population 60 days after the infection was initiated.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology; D006224:Cricetinae; D003593:Cytoplasm / Q000382:microbiology; D004285:Dogs; D006389:Hemagglutinins, Viral / Q000096:biosynthesis; D007372:Interferons / Q000096:biosynthesis; D009154:Mutation; D009522:Newcastle disease virus / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D013696:Temperature; D013997:Time Factors; D014776:Virus Cultivation; D014779:Virus Replication
D006389:Hemagglutinins, Viral; D012367:RNA, Viral; D007372:Interferons