Separation of 1,2-dimethylhydrazine metabolites by high-pressure liquid chromatography.
The separation of 1,2-dimethylhydrazine, azomethane, azoxymethane, methylazoxymethanol, methylazoxymethanol acetate, formaldehyde and methanol by high-pressure liquid chromatography on columns of C18/Corasil, muBondapak C18 and Aminex A-27 is described. The separations are highly reproducible and rapid and may be used for kinetic studies. An example of an application of these methods to the analysis of metabolites in rat urine derived from (14C)-1,2-dimethylhydrazine is reported.
D000818:Animals; D001391:Azo Compounds / Q000032:analysis*; D001397:Azoxymethane / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004127:Dimethylhydrazines / Q000378:metabolism; D005557:Formaldehyde / Q000032:analysis; D007700:Kinetics; D008697:Methane / Q000031:analogs & derivatives; D000432:Methanol / Q000031:analogs & derivatives / Q000032:analysis; D051381:Rats
D001391:Azo Compounds; D004127:Dimethylhydrazines; D005557:Formaldehyde; D001397:Azoxymethane; D008697:Methane; D000432:Methanol