The lecithin: cholesterol acyltransferase reaction, lysolecithin and red cell ageing in blood stored under normal transfusion service conditions.
The lecithin:cholesterol acyltransferase (LCAT) activity and lysolecithin content of human blood stored under standard blood transfusion service conditions at 4 degrees C for 6 weeks has been investigated. Cooling the blood to 4 degrees C rapidly inactivates the LCAT reaction, but the enzyme is not denatured during storage under these conditions. Citrate in the anticoagulant did not activate the LCAT reaction in freshly-taken whole blood. The total phospholipid and total lysolecithin content of whole blood decreased during storage at 4 degrees C for 6 weeks. The lysolecithin content of fresh red cells (2.0-3.0 mumol lysolecithin x 10(-11) per cell) showed no significant change during the storage period.
D000217:Acyltransferases / Q000097:blood*; D001771:Blood Banks; D001793:Blood Preservation / Q000379:methods*; D002951:Citrates / Q000494:pharmacology; D004905:Erythrocyte Aging; D006801:Humans; D008244:Lysophosphatidylcholines / Q000097:blood*; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood*; D010743:Phospholipids / Q000097:blood; D013997:Time Factors
D002951:Citrates; D008244:Lysophosphatidylcholines; D010743:Phospholipids; D000217:Acyltransferases; D007862:Phosphatidylcholine-Sterol O-Acyltransferase