Damaged DNA in lymphocytes of aplastic anemia.
The size of single-stranded DNA in lymphocytes in GO stage from 22 patients with acquired primary and secondary aplastic anemia was estimated by alkaline sucrose gradient centrifugation. The average size was 9.3 (+/-0.3) times 10(7) daltons. The lymphocytes of patients contained significantly more single-strand breaks in DNA, compared to those of normal persons. The difference in size of single-stranded DNA which had been present in nontransformed lymphocytes could also be observed in transformed lymphocytes. Some characteristic differences could be observed in the sedimentation patterns of single-stranded DNA in the lymphocytes of patients with aplastic anemia and those of normal persons. The single-strand breaks in DNA suggested that the repair processes were disturbed in the DNA molecules of circulating lymphocytes from patients with acquired primary and secondary aplastic anemia.
D000741:Anemia, Aplastic / Q000097:blood*; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004277:DNA, Single-Stranded / Q000032:analysis*; D006801:Humans; D007942:Leukemia, Experimental / Q000097:blood; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000032:analysis*; D008970:Molecular Weight
D004277:DNA, Single-Stranded