Neonatal androgenization: effects of responsiveness of ovarian alkaline phosphatase to gonadotropins.
Five-day-old female mice were injected subcutaneously with 100 mug of testosterone benzoate in oil, or with oil only. At various ages thereafter, they received either 5 I.U. human chorionic gonadotropin (hCG) per 10 g body weight or saline only, and were killed 24 h later. Alkaline phosphatase activity was measured in whole ovarian homogenates. Neonatal androgenization failed to affect the early phases of ovarian responsiveness, but selectively abolished both the normal rise in alkaline phosphatase which precedes the onset of puberty and the responsiveness of the enzyme to hCG stimulation at this time.
D000375:Aging; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D005260:Female; D051379:Mice; D010053:Ovary / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013739:Testosterone / Q000494:pharmacology*
D006063:Chorionic Gonadotropin; D013739:Testosterone; D000469:Alkaline Phosphatase