Cell cycle dependent exposure of a high molecular weight protein on the surface of mouse L cells.
The non-penetrating lactoperoxidase iodination probe has been employed in conjunction with synchronously dividing populations of mouse L cells to identify a high molecular weight protein is preferentially exposed on the L cell surface during G1 phase of the cell cycle. Progression of cells from G1 to S is accompanied by a marked decrease in tha availability of this structure, called band 1, to lactoperoxidase-catalyzed iodination and it remains unavailable until cells re-enter G1. It is suggested that the band 1 polypeptide may be functionally involved in the regulation of L cell growth.
D000818:Animals; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D007739:L Cells / Q000378:metabolism*; D007784:Lactoperoxidase; D051379:Mice; D008970:Molecular Weight; D011506:Proteins / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D011506:Proteins; D007784:Lactoperoxidase; D013936:Thymidine