[The "protective" effect of gamma-irradiation of cells in metaphase of mitosis following V-irradiation during the S period].
20,1% cells with chromosomes aberrations were obtained after UV-irradiation of embryonal fibroblasts of mice at the S-stage in vitro at a decreasing dose of 40erg/mm2. Subsequent gamma-irradiation at the metaphase of the first mitosis at a 5 krad dose led to a statistically significant decrease of the frequency of aberrant cells observed in the same mitosis down to 11,7%. The frequency of spontaneous aberrations did not change during the first few minutes after gamma-irradiation of intact cells at the metaphase. The "protective" effect of gamma-rays can not be explained either by unequal changes of the duration of mitotic stages for aberrant and normal cells, or by sticking of chromosome fragments or by breaks of bridges at the anaphase. The death of cells "under irradiation" also appears to be a hardly probable case of the effect observed. It is assumed that the decrease of the aberrations frequency is the result of predicted earlier modification of the processes of realization of potential chromosome damages into visible aberrations at the metaphase.
D000818:Animals; D002869:Chromosome Aberrations; D005347:Fibroblasts; D005720:Gamma Rays; D051379:Mice; D008807:Mice, Inbred BALB C / Q000196:embryology; D008938:Mitosis / Q000528:radiation effects*; D011831:Radiation Genetics; D014466:Ultraviolet Rays
"Zashchitnyĭ" éffekt gamma-oblucheniia kletok v metafaze mitoza posle UF-oblucheniia v S-periode