Inhibition of the in vivo effects of concanavalin-A on mammalian epidermis by alpha-methyl-D-glucopyranoside.
Concanavalin-A (Con-A) injected intradermally into newborn rats produces inhibition of granular-cell formation, accumulation of spinous cells, glycogen deposition, and a decrease followed by an increase in the number of basal cells in DNA synthesis. These changes were maximal with a dose of 0.1 mg Con-A, although 0.005, 0.01, and 0.05 mg caused some epidermal changes. The Con-A effects were partially blocked when 0.1 ml of 0.3 or 0.1 M alpha-methyl-D-glucopyranoside (alphaMG) solution was injected 2 hr after 0.1 mg Con-A and completely inhibited by injection of 0.1 ml of 3.0 M alphaMG solution. The inhibitory effects were not seen after injection of 0.1 ml of 3.0 M N-acetyl-galactosamine saline solution, or 0.1 ml normal saline. Injection of alphaMG alone did not cause any changes in epidermal cells. These results indicate that specific sugar inhibits Con-A effects on mammalian epidermis in vivo.
D000117:Acetylglucosamine / Q000494:pharmacology; D000818:Animals; D003208:Concanavalin A / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006003:Glycogen / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D008759:Methylglycosides / Q000494:pharmacology*; D014176:Protein Biosynthesis; D051381:Rats; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D008759:Methylglycosides; D003208:Concanavalin A; D006639:Histidine; D006003:Glycogen; D004247:DNA; D000117:Acetylglucosamine; D013936:Thymidine