title: The function of slime from Physarum flavicomum in the control of cell division.
abstract: A haploid cell of the myxomycete Physarum flavicomum undergoes cytokinesis, producing a large population of cells. However, after syngamy, cytokinesis no longer occurs but karyokinesis does and subsequent growth results in the formation of a diploid syncytial plasmodium. Slime, which is produced by the plasmodium but not the haploid cells, was aseptically isolated and purified, and tested for its effect as a cytokinetic regulator. Slime (a viscous, high molecular weight, acidic glycoprotein) affected cytokinesis of the haploid myxamoebae growing in pure culture in soluble media, and the effect was concentration dependent. In simple media, a slime concentration of about 6  10(-5) mug protein per cell suppressed cytokinesis about 50%, unequally inhibited the synthesis of protein, RNA, and DNA, but stimulated respiration. The biological activity of slime was not species specific and it also affected the bacterium Bacillus subtilis by inhibiting cytokinesis, stimulating oxygen uptake, and producing an aberrant cell morphology. Slime was inactivated by heat, fragmentation, and incubation with dithiothreitol, mercaptoethanol, and the proteolytic enzyme papain (EC 3.4.22.2). The inhibitory effect of slime on cell division of haploid cells could not be achieved using mucin or various polyanions. The possible role of slime in the production of the diploid syncytium is discussed.
mesh_terms: D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004171:Diploidy; D004229:Dithiothreitol / Q000494:pharmacology; D005656:Fungal Proteins / Q000096:biosynthesis / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis / Q000494:pharmacology; D006238:Haploidy; D006358:Hot Temperature; D008623:Mercaptoethanol / Q000494:pharmacology; D009235:Myxomycetes / Q000254:growth & development*; D010101:Oxygen Consumption / Q000187:drug effects; D010206:Papain / Q000494:pharmacology; D010804:Physarum / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis
chemical_list: D005656:Fungal Proteins; D006023:Glycoproteins; D008623:Mercaptoethanol; D012313:RNA; D004247:DNA; D010206:Papain; D004229:Dithiothreitol