[Preparation of isolated cells by alkaline dissociation of formalin fixed tissue].
A procedure is described for preparation of isolated cells by treating formaldehyde fixed tissues with a 50%-solution of KOH. This results in complete yield of cells from a variety of organs (liver, kidney, heart, spleen, etc.). The alkali-treated cells entirely retain their morphological and tinctorial peculiarities. It was shown that preparations derived from alkali-treated tissues were useful for a series of quantitative cytological and cytochemical techniques: cytofluorometric estimation of nuclear DNA; interferometric determination of dry cell mass; autoradiographic studies of nuclear DNA synthesis; cell number counts; evaluation of cell distribution according to the number of their nuclei; estimation of the mitotic index.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D007908:Lens, Crystalline / Q000166:cytology; D008099:Liver / Q000166:cytology; D008115:Liver Regeneration; D051379:Mice; D009206:Myocardium / Q000166:cytology; D010179:Pancreas / Q000166:cytology; D011689:Purkinje Cells
Poluchenie izolirovannykh kletok metodom shchelochnoĭ dissotsiatsii fiksirovannykh formalinom tkaneĭ