[Preservation of tissue culture cells by deep freezing].
The effect of polyethylene oxide, mol. wght 400 and 4000, the rate of freezing in liquid nitrogen, and thawing in a water bath at 37 degrees C on the survival rate, the growth activity, miotic activity and the activity of some oxidation-reduction enzymes of the most important links of the chain of biological oxidation, SDG, NAD-H2, NADP-H2- diaphorases, electrophoretic motility of continuous human and animal tissue culture cells, RH HEp-2, SPEV were studied. The developed regimen of freezing at a rate of 30 degrees per minute retains 95% viable cells. The miotic activity, enzymatic activity and electrophoretic motility of frozen-thawed cells did not differ from those of the controls.
D002470:Cell Survival; D005615:Freezing; D014021:Tissue Preservation / Q000379:methods*
Konservirovanie kletok kul'tury tkaneĭ metodome glubokogo zamorazhivaniia