Purification and characterization of dopamine beta-hydroxylase from bovine adrenal medulla.
A new purification procedure that permits large-scale purification of dopamine beta-hydroxylase from bovine adrenal medulla was developed. Whole adrenal medullas were extracted with 0.1% Triton X-100, and the enzyme was purified by precipitation with polyethylene glycol, chromatography on DEAE-cellulose, and adsorption to concanavalin A linked to agarose. The yield of protein and the specific activity were high compared with previously published methods. The enzyme appeared essentially homogenous by the criteria of polyacrylamide gel electrophoresis in the presence or absence of dodecylsulfate, and sedimentation velocity analysis. The purified protein was subjected to amino acid and carbohydrate analyses, and the results were compared with previously published data. We found about 3 mol of copper per mol of protein (tetramer of 290000 daltons). No free sulfhydryl groups could be found. Analysis for NH2-terminal amino acids with [14C]dansyl chloride revealed 2 residues of alanine and 2 residues of serine per tetramer. We found the NH2-terminal amino acid of chromogranin A to be leucine. The results of our analysis for amino acid composition and NH2-terminal amino acids do not support the suggestion that dopamine beta-hydroxylase and chromogranin A contain identical peptide chains.
D000313:Adrenal Medulla / Q000201:enzymology*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D003300:Copper / Q000032:analysis; D003594:Cytoplasmic Granules / Q000201:enzymology; D004299:Dopamine beta-Hydroxylase / Q000032:analysis / Q000302:isolation & purification*; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis
D000596:Amino Acids; D006595:Hexosamines; D006601:Hexoses; D012794:Sialic Acids; D003300:Copper; D004299:Dopamine beta-Hydroxylase