title: The biosynthesis of transfer RNA in insects. I. Increase of amino acid acceptor activity of specific tRNA's utilized for silk protein biosynthesis in the silk gland of Bombyx mori.
abstract: 1) To detect the quantitative changes of amino acid acceptor activity of tRNA's from the posterior and middle silk glands of Bombyx mori at various ages, a relatively simple and rapid method was established using a mixture of radioactive amino acids in Chlorella hydrolysate. 2) The acceptor activities of silk gland tRNA for 15 amino acids tested seemed to be almost on the same level at the end of the 4th moult stage. During the 5th instar, however, characteristic increases were observed in glycine, alanine, and serine acceptor activities in both silk glands. 3) In the posterior silk gland, which produces fibroin, the acceptor activities for glycine and alanine increased more than that for serine. In the middle silk gland, which produces sericine, the acceptor activity for serine increased more than those for glycine and alanine. 4) In the light of observations on the increase of corresponding aminoacyl-tRNA synthetase activities in the silk glands, a functional adaptation of tRNA synthesis in the tissue is discussed.
mesh_terms: D000412:Alanine-tRNA Ligase / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases; D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D006032:Glycine-tRNA Ligase / Q000378:metabolism; D007700:Kinetics; D007814:Larva; D007935:Leucine-tRNA Ligase / Q000378:metabolism; D008675:Metamorphosis, Biological; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis*; D012714:Serine-tRNA Ligase / Q000378:metabolism; D013997:Time Factors; D046249:Transfer RNA Aminoacylation
chemical_list: D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D012714:Serine-tRNA Ligase; D006032:Glycine-tRNA Ligase; D007935:Leucine-tRNA Ligase; D000412:Alanine-tRNA Ligase