title: Revision of estimates of the molecular weights of tobravirus coat proteins.
abstract: The molecular weights of proteins from several tobacco rattle viruses (or tobraviruses) have been determined by electrophoresis with 'marker' proteins in acrylamide gels of different strengths. Plotting KR, a measure of the effect of varying the gel strength, against molecular weight gave a value of 23,000 +/- 2,800 for tobacco rattle virus (TRV) strain CAM and similar values for other strains of TRV and pea early-browning virus. A value of 21,700 +/- 700 was obtained for TRV strain PRN by sedimentation to equilibrium in 6 M guanidine hydrochloride. These estimates are similar to those made by a statistical reappraisal of published data on amino acid composition, and a value of about 22,000 is proposed for TRV coat proteins.
mesh_terms: D008970:Molecular Weight; D010942:Plant Viruses / Q000032:analysis*; D010947:Plants, Toxic; D014026:Nicotiana; D014764:Viral Proteins / Q000032:analysis*
chemical_list: D014764:Viral Proteins