Messenger RNA induction of fast sodium ion channels in cultured cardiac myoblasts.
Incubation with adult heart messenger RNA caused the appearance of fast sodium ion channels in young myocardial cells whose development has been arrested in vitro. The induction was blocked by cycloheximide, indicating dependence on protein synthesis. Thus, cardiac myoblasts can be made to differentiate in vitro, and membrane properties can be altered by exogenous RNA.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001692:Biological Transport; D002454:Cell Differentiation / Q000187:drug effects; D002642:Chick Embryo; D003513:Cycloheximide / Q000494:pharmacology; D006321:Heart / Q000196:embryology* / Q000502:physiology; D007700:Kinetics; D009124:Muscle Proteins / Q000096:biosynthesis; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D009124:Muscle Proteins; D012333:RNA, Messenger; D013779:Tetrodotoxin; D003513:Cycloheximide; D012964:Sodium