Variation of serum iron concentration in young healthy men: Within-day and day-to-day changes.
1. Serum iron concentration values were determined on a group of 11 healthy young men on blood specimens which were drawn at various hours of the day: 800 h, 1100 h, and 1400 h; on each of four separate days. 2. using a three-way ANOVA (analysis of variance) model, we determined the diurnal variation both for the group as a whole (main hour effect) and for the individual subjects (subject-hour and subject-day-hour interactions). 3. the total combined within-day variation as expressed in coefficients of variation was 12.9% with the peak value seen at 1400 h. 4. on a separate study, blood specimens were obtained at 1100 h on five separate days during a two week period on the same volunteers. 5. using a two-way ANOVA model (subject and day) the day-to-day coefficient of variation was found to be 26.6% for the group. In both cases (within-day and day-to-day) the biological variation was considerably greater than the analytic variation.
D000328:Adult; D002940:Circadian Rhythm; D003201:Computers; D006801:Humans; D007501:Iron / Q000097:blood*; D008297:Male; D010507:Periodicity
D007501:Iron