[Countercurrent distribution of polysulfonated proteins (author's transl)].
The reaction of bovine beta-lactoglobulin AB with reagents 4-(isothiocyanato) benzene sulfonic acid, 5-(isothiocyanato) benzol-1,3-bis(sulfonic acid) and 7-(isothiocyanato) naphthalene-1,3,5-tris(sulfonic acid) is described. The blocking of the epsilon-amino groups of lysine is quantitative. The thus modified protein can be analysed in the countercurrent distribution apparatus and can be split very rapidly at the arginine residues with trypsin.
D000818:Animals; D001557:Benzenesulfonates; D001665:Binding Sites; D002417:Cattle; D003377:Countercurrent Distribution / Q000379:methods; D007782:Lactoglobulins / Q000302:isolation & purification*; D011485:Protein Binding; D013861:Thiocyanates; D014357:Trypsin
D001557:Benzenesulfonates; D007782:Lactoglobulins; D013861:Thiocyanates; D014357:Trypsin
Gegenstromverteilung von poly-sulfonierten Proteinen