Simple method for the isolation and purification of hemoglobin components.
A simple method for the isolation and purification of hemoglobin components from starch gel by electrophoresis is described. An inverted bottle with a cut off bottom is used. Pieces of gel containing the hemoglobin are embedded in potato starch, wetted with buffer, and placed in the neck of the inverted bottle, the mouth of which has been closed with filter paper and tape. Connection with the negative tank buffer is achieved by inserting a layer of starch gel between the potato starch and the negative tank buffer. By electrophoresis, the hemoglobin migrates and accumulates in a dialysing tube tied around the mouth of the bottle and hanging into the positive tank buffer.
D002021:Buffers; D003956:Dialysis; D004592:Electrophoresis, Starch Gel; D006454:Hemoglobins / Q000302:isolation & purification*; D008722:Methods
D002021:Buffers; D006454:Hemoglobins