Comparison of IgE values as determined by different solid phase radioimmunoassay methods.
Accurate measurement of low IgE concentrations if technically difficult. In this paper results obtained by a direct sandwich and three inhibition methods of radioimmunoassays are compared. For values above 50 U/ml good correlation was obtained with all methods. Below 50 U/ml, however, the inhibition methods tended to yield falsely high values. For very low concentrations, 1--10 U/ml the best correlation was obtained between the direct sandwich test (PRIST) and the inhibition test using a correction factor to allow for the non-specific effect of serum. The four methods were used to quantify IgE in cord serum samples from healthy individuals. The mean value obtained by PRIST was 0-4 U/ml and by the inhibition test, using a correction factor, 0-6 U/ml respectively. Because of its greater simplicity the direct sandwich test is recommended.
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D007223:Infant; D007231:Infant, Newborn; D011863:Radioimmunoassay / Q000379:methods*
D007073:Immunoglobulin E