[The effect of inhibition of protein synthesis on some biochemical and functional activities of the brain in chick embryos].
By means of radioautographic and biochemical methods, it has been shown that cycloheximide administration to the brain in a dose of 76 mug significantly decreases protein synthesis within 30 min, the decrease being maximal after 2 hours. RNA synthesis in neuronal cytoplasm is blocked within 4 hours. Changes in biopotentials of the forebrain and midbrain structures were observed already 15-20 minutes after cycloheximide treatment. Application of cycloheximide (12.5-25 mug) to the area of vascular brannching of the foetal membranes of a 17-day embryo inhibits motor activity within 20 min.; in 28-day embryos, this effect is observed at a dose of 75 mug. Therefore, inhibition of protein synthesis in the brain results in sharp changes of the electrical activity of the brain and inhibits motor activity of the chick embryo.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D002642:Chick Embryo; D003513:Cycloheximide / Q000494:pharmacology*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004558:Electric Stimulation; D014176:Protein Biosynthesis / Q000187:drug effects*; D014158:Transcription, Genetic / Q000187:drug effects
D003513:Cycloheximide
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