DNA-synthesis in synchronized L-cells after irradiation during the G1-phase of the cell cycle.
Mouse fibroblast L-929 cells synchonized by mitotic selection were irradiated during the G1-phase of the cell cycle with a dose of 1000 rad. The rat of DNA synthesis was measured by 3H-thymidine incorporation, and the progression of the cells through the cell cycle was determined using a pulse-cytophotometer. Irradiation caused a decrease in the rate of DNA synthesis to half the control value, and an extension of the S-phase to twice its normal duration.
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis*; D007739:L Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D051379:Mice; D011830:Radiation Effects; D014965:X-Rays
D004247:DNA