Asymmetry and transposition rates of phosphatidylcholine in rat erythrocyte ghosts.
Purified phospholipid exchange protein from beef heart cytosol is used to accelerate the exchange of phospholipids between labeled sealed ghosts and phosphatidylcholine/cholesterol liposomes. The purified protein accelerates the transfer of phosphatidylcholine and, to a lesser degree, that of sphingomyelin, phosphatidylinositol, and lysophosphatidylcholine. The presence of exchange protein does not accelerate the exchange of phospholipids between intact red blood cells and liposomes, but 75% of the phosphatidylcholine of sealed ghosts is readily available for exchange. The remaining 25% is also exchangeable but at a slower rate. When the exchange is assayed between inside-out vesicles and liposomes, 37% of the phosphatidylcholine is readily available, and 63% is exchanged at a slower rate. These results are consistent with an asymmetric distribution of phosphatidylcholine in isolated erythrocyte membrane fractions. The sum of the forward and backward transposition of phosphatidylcholine between the inside and outside layers of sealed ghost membranes amounts to 11% per hour, and the half-time for equilibration is 2.3 h. Significatnly lower values are obtained for the inside-out vesicles (half-time for equilibration: 5.3 h). These results suggest that, during the formation of the vesicles, the asymmetry of phosphatidylcholine is partially preserved, but structural changes occur in the membrane that affect the rate of membrane transposition of phosphatidylcholine.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002784:Cholesterol; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D008081:Liposomes; D008297:Male; D008433:Mathematics; D008567:Membranes, Artificial; D008954:Models, Biological; D010713:Phosphatidylcholines / Q000097:blood*; D010743:Phospholipids / Q000097:blood; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats
D008081:Liposomes; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D010743:Phospholipids; D011092:Polyethylene Glycols; D002784:Cholesterol