The determination of gamma-amino-beta-(p-chlorophenyl)butyric acid (baclofen) in biological material by gas-liquid chromatography.
Baclofen and the internal standard are isolated from biological samples by adsorption on charcoal. After elution, the compounds are converted into butyl esters and purified by base-specific solvent extraction. The butyl esters are then N-acylated with heptafluorobutyrylimidazole, and the neutral derivatives are isolated by solvent extraction and subjected to gas-liquid chromarography, with use of an electron-capture detector. Baclofen can be determined in concentrations down to about 30 ng per sample.
D000215:Acylation; D000613:Aminobutyrates / Q000032:analysis*; D001418:Baclofen / Q000032:analysis* / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D008832:Microchemistry; D012997:Solvents
D000613:Aminobutyrates; D007202:Indicators and Reagents; D012997:Solvents; D001418:Baclofen