[Evaluation of various radioimmunological techniques for the detection of antigen HBs].
The different radioimmunoassay technics routinely used for detection of HBs Antigen are Ausria I, Ausria II, RIA and CLB/RIA. Classification of these techniques either liquid or solide phase, is realized by nature of the tracer (Antigen or Antibody radioiodinated). The sensitivity and the specificity are presented as the comparison with other reactions. There is no significative difference on 1089 tested samples between Ausria I and RIA. The percentage of false positives in RIA or CLB/RIA reactions is 1.5% when the incubation is at 20 degrees C and 1% when it is at 37 degrees C. Advantages, disadvantages, cost prices, automation and possibility of computer treatment are discussed. The utilization of reference sera is proposed and the problem of the utilization en France of the radioactive products is approached.
D006511:Hepatitis B Antigens / Q000302:isolation & purification*; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*
D006511:Hepatitis B Antigens
Etude critique des différentes techniques radioimmunologiques de détection de l'antigène HBs