[Radioimmuno assay of the sulfonylurea AR-DF 26(author's transl)].
A radioimmunological method was developed for 1-cyclohexyl-3-[p-(beta-4,4-dimethyl-7-methoxy-1,3-(2H,4H)-isoquinolinedion-2-yl)phenethyl]-sulfonylurea (AR-DF 26, gliquidone, Glurenorm) estimations in plasma. The antisera were obtained by immunization of rabbits with AR-DF 26-bovine serum albumin (BSA) conjugate. The estimation is based on the competitive displacement in plasma samples of known amounts of labelled AR-DF 26, bound to a specific antibody by unknown amounts of unlabelled AR-DF 26. Concentrations of as low as 1 ng of AR-DF 26 per sample can be measured by this method. The investigations into the specificity of this reaction have shown that the antiserum is extremely specific for AR-DF 26. There is good reproducibility of the results and no differences could be found by variance analysis between different measurements of the same AR-DF 26 concentration. Since more than 70% of the plasma concentration correspond to unchanged AR-DF 26 for a longer period after application, the results of this AR-DF 26-radioimmuno assay is only minimally altered by its metabolites.
D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D003429:Cross Reactions; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood* / Q000276:immunology; D007106:Immune Sera; D007546:Isoquinolines / Q000097:blood / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D012710:Serum Albumin, Bovine; D013453:Sulfonylurea Compounds / Q000097:blood* / Q000276:immunology
D000936:Antigen-Antibody Complex; D007004:Hypoglycemic Agents; D007106:Immune Sera; D007546:Isoquinolines; D013453:Sulfonylurea Compounds; D012710:Serum Albumin, Bovine
Radioimmunologische Bestimmung des Sulfonylharnstoffes AR-DF 26