Cleavage of cardiac steroid glucosides by pure beta-glucosidase from Aspergillus ventii.
Electrophoretically pure beta-glucosidase (beta-glucoside glucohydrolase, EC 3.2.1.21) A3 from Aspergillus wentii was shown to deglucosylate cardiac steroid biosides, triosides and tetraosides with a terminal beta-D-glucoside residue linked to the aglycon sugar in 1 leads to 6 or 1 leads to 4 position. In dependence on the structure and conformation of the aglycon, the deglucosylation rate may vary by six orders of magnitude.
D001230:Aspergillus / Q000201:enzymology*; D002301:Cardiac Glycosides / Q000378:metabolism*; D002474:Cell-Free System; D005959:Glucosidases / Q000378:metabolism*; D006868:Hydrolysis
D002301:Cardiac Glycosides; D005959:Glucosidases