Studies on the low molecular weight RNA associated with 28S ribosomal RNA from Crotalus durissus terrificus liver.
A low molecular weight RNA was released from the purified rattlesnake 28 S RNA by brief heat treatment as well as by treatment with 80% dimethylsulfoxide or formamide. The sedimentation coeficient of this low molecular weight RNA was found to be 5.5 S, corresponding to a nucleotide number of 140 and a molecular weight of 46 000. It was also observed that 5.5S RNA is present in equimolar ratio to 5 S rRNA. Heat treatment of the purified 60 S ribosomal subunit also released the 5.5 S RNA. The possibility that this low molecular weight RNA is located on the surface of the large ribosomal subunit is discussed.
D000818:Animals; D001665:Binding Sites; D004121:Dimethyl Sulfoxide; D005559:Formamides; D006358:Hot Temperature; D008099:Liver / Q000032:analysis*; D008970:Molecular Weight; D012313:RNA; D012335:RNA, Ribosomal; D012911:Snakes; D013696:Temperature
D005559:Formamides; D012335:RNA, Ribosomal; D012313:RNA; D004121:Dimethyl Sulfoxide