Immunoadsorbents: non-specific binding of proteins to albumin-sepharose.
Human serum albumin-Sepharose was prepared by coupling human serum albumin to cyanogen bromide activated Sepharose 4B. This immunoadsorbent showed considerable non-specific protein adsorption. The adsorbed proteins were mainly immunoglobulins which could not be separated from required antibody. It is suggested that basic groups formed in the preparation of the albumin-Sepharose are responsible for this non-specific protein adsorption. Non-specific protein adsorption could be completely eliminated by neutralizing the basic groups of the albumin-Sepharose with the anionic dye blue dextran. The resultant blue dextran-albumin-Sepharose allowed purification of anti-albumin-antibody with high yield. It is shown that the isolated antibody is pure and retains its native properties.
D000327:Adsorption; D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification; D000918:Antibody Specificity; D006801:Humans; D011134:Polysaccharides; D011485:Protein Binding; D012685:Sepharose; D012709:Serum Albumin / Q000276:immunology*
D000906:Antibodies; D011134:Polysaccharides; D012709:Serum Albumin; D012685:Sepharose