title: Reiteration frequency of the gene for tissue-specific histone H5 in the chicken genome.
abstract: Chicken erythroid cells contain a tissue specific histone known as H5 in addition to the five major histone species found in other organisms. The mRNA coding for this histone has been isolated by indirect immunoprecipitation from immature, non-dividing reticulocytes in which this is the only histone synthesised. The mRNA has been modified by the enzymatic addition of a 3' polyadenylic acid tract, and transcribed into complementary DNA (cDNA) using the RNA-dependent DNA-polymerase from avian myeloblastosis virus. Studies on the hybridisation of this cDNA indicate that the gene coding for the H5 histone is reiterated 10 times in the chicken genome.
mesh_terms: D000483:Alleles; D000818:Animals; D002645:Chickens; D004247:DNA / Q000096:biosynthesis; D005796:Genes; D006657:Histones / Q000096:biosynthesis*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000302:isolation & purification
chemical_list: D006657:Histones; D012333:RNA, Messenger; D004247:DNA