Tryptic peptides from the beta polypeptide chain of AII component of chicken hemoglobin.
The aminoethylated beta polypeptide chain in AII component from the hemoglobin of adult chicken was digested with trypsin [EC 3.4.21.4] and the resulting peptides were separated and purified by ion exchange chromatography, paper chromatography, and gel filtration. Eighteen tryptic peptides, which were nonoverlapping, accounted for all of the amino acid residues in the beta polypeptide chain. The amino acid sequences of the tryptic peptides were established by a combination of enzymatic digestion and subtractive Edman degradation.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002268:Carboxypeptidases; D002645:Chickens; D002854:Chromatography, Paper; D002918:Chymotrypsin; D006454:Hemoglobins; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000032:analysis; D014357:Trypsin
D000596:Amino Acids; D006454:Hemoglobins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D002268:Carboxypeptidases; D002918:Chymotrypsin; D014357:Trypsin