title: The efficacy of killed Trypanosoma evansi vaccines in mice.
abstract: Four strains of Trypanosoma evarsi (D3, D4, D5 and D6) isolated from German shepherd dogs were inoculated into mice, and infected blood was used to prepare 9 separate killed vaccines. White mice inoculated with 1:100 diluted PBS vaccine, 0.5% carbol vaccine, or 100% Lugol vaccine showed survival rates of more than 60%. Among these 3 vaccines PBS vaccine and 0.5% carbol vaccine showed higher survival rates at 1:500 and 1:1000 dilutions, respectively. When young mice (15-20 g) were immunized with PBS vaccine, they resisted challenge with homologous strains, D3 strain in single injection, D6 strain in double injections and all strains in 5 injections. Protection however was not observed in old mice (25-30 g) give the same vaccine preparation. When mice were vaccinated with a single injection of D3 vaccine and challenged with heterologous strains, only those challenged with D4 strain at 10-5 dilution showed a survival rate of more than 60%. There was no difference in protective ability among PBS vaccine, agar adjuvant and kaolin adjuvant vaccines. Agglutinating antibody was demonstrated in mice receiving 5 injections of PBS vaccine.
mesh_terms: D000367:Age Factors; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D005069:Evaluation Studies as Topic; D051379:Mice; D013045:Species Specificity; D014345:Trypanosoma / Q000276:immunology*; D014352:Trypanosomiasis / Q000517:prevention & control*; D014612:Vaccines
chemical_list: D000906:Antibodies; D014612:Vaccines