[DNA replication and the development of spontaneous mutations in the somatic cells of mammals. I. The relationship between the outcome of gene mutations and the duration of cell life].
The reduction of serum concentration in culture medium to 0,1% was proposed as a method of slowing division and DNA replication in Chinese hamster cells in vitro. Under these conditions the rate of DNA replication was reduced by more than one order of magnitude, while cell viability remained high enough (about 10%) for a long period up to 30 days. The spontaneous mutation rate to 6-mercaptopurine resistance, as calculated per day in cells with reduced DNA replication rate, was comparable to that in normally dividing cells [(1,2 +/- 0,3) X 10(-5) and (3,1 +/- 0,5) X 10(-5) per cell per day respectively]. This proves that a considerable fraction of spontaneous mutants arise independently on DNA replicative synthesis.
D000818:Animals; D002999:Clone Cells; D006224:Cricetinae; D004261:DNA Replication; D009154:Mutation
Vozniknovenie spontannykh mutatsiĭ v somaticheskikh kletkakh mlekopitaiushchikh i replikatsiia DNK. I. Zavisimost' vykhoda gennykh mutatsiĭ ot dlitel'nosti zhizni kletok