An experimental study of acute and chronic effects of phenacetin on the rat kidney, using clinical-chemical and biochemical methods.
In rats, changes in urinary enzymatic activities (AP, SP, LAP, beta-GLU, MUR) were recorded following the administration of phenacetin in acute doses (4.75 and 7.15 mmol/kg). Urinary AP and LAP activities were measured over 77 days in which 3.35 mmol phenacetin/kg were given daily. The results revealed immediate and delayed effects of phenacetin, depending upon the quality of the drug used. In the chronic series, changes in urinary enzymatic activities were less pronounced. Concomitant biochemical investigations of kidney cell fractions revealed the occurrence of mitochondrial damage under the influence of chronic phenacetin administration. Following acute doses of phenacetin, destructive alterations in the plasma membrane of kidney cells were encountered. Investigations of serum enzymatic activities 24 h after phenacetin administration did not reveal any significant changes.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005966:Glucuronidase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008566:Membranes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D010615:Phenacetin / Q000494:pharmacology*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D005993:Glycerolphosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D000469:Alkaline Phosphatase; D005966:Glucuronidase; D007931:Leucyl Aminopeptidase; D010615:Phenacetin