[Synthesis of some aminoacyl-AH-sepharoses via the N-carboxyanhydride method (author's transl)].
(Aminoacyl)n=1-10-AH-Sepharoses can easily be prepared by the N-carboxy anhydride method from amino acid N-carboxy anhydrides and AH-Sepharose 4B at pH 10.2, 0degreesC in aqueous borate buffer. Syntheses of cystinyl-, cysteinyl-, isoleucyl-, leucyl- and phenylalanyl-AH-Sepharoses are described. An improved preparation for cystine bis(N-carboxy anhydride) is presented.
D000596:Amino Acids; D000812:Anhydrides; D001665:Binding Sites; D008722:Methods; D011134:Polysaccharides / Q000138:chemical synthesis*; D012685:Sepharose / Q000031:analogs & derivatives / Q000138:chemical synthesis*
D000596:Amino Acids; D000812:Anhydrides; D011134:Polysaccharides; D012685:Sepharose
Synthese einiger Aminoacyl-AH-Sepharosen mit der N-Carboxyanhydrid-Methode