[Characteristics of human embryonal hepatocytes cultivated in vitro].
A simple and reproducible method for cultivation of human embryo (7--12-week) hepatocytes in a monolayer is presented. The culture showed the capacity for limited growth, displayed a viability for 4 to 6 weeks without being subcultured, and retained some specific characteristics: synthesis of serum protein (albumin and alpha-fetoprotein), a significant amount of glycogen, a high activity of monaminooxidase, and a characteristics response to a high concentration of glucocorticoids. These properties are useful as markers for genetic experiments or for selection.
D002478:Cells, Cultured; D006651:Histocytochemistry; D006801:Humans; D008099:Liver / Q000166:cytology / Q000196:embryology*
Kharakteristike émbrional'nykh gepatotsitov cheloveka, kultiviruemykh in vitro