Alterations in steroid and gonadotropin release resulting from surgical stress during the morning of proestrus in 5-day cyclic rats.
The purpose of this experiment was to determine whether surgical stress on the morning of proestrus would elicit an early release of gonadotropin from the pituitary. Animals exhibiting 5-day estrous cycles underwent bilateral sham-ovariectomy under ether anesthesia at 0800 h of proestrus. These animals had high levels of progesterone and estradiol following the surgery. These steroids were thought to be adrenal in origin, since animals adrenalectomized at 0800 h of proestrus had low progesterone levels and estradiol comparable to unoperated controls. Subsequently, the sham-operated animals showed high FSH but not LH values at 1300 h, prior to the normal critical period for gonadotropin release. By 1400, the LH surge had begun, and progesterone was again being released. Adrenalectomized and unoperated controls showed no increase in any steroid or gonadotropin measured before 1400 h. These findings suggest that stress-induced release of adrenal estradiol and progesterone, rather than some other consequence of the surgical procedure, during the morning of proestrus, can advance the onset of release of FSH, prior to LH. Ovariectomy at 0800 h proestrus led to a rapid and dramatic increase in FSH but not LH secretion by 4 h after surgery. By 6 h after ovariectomy FSH had increased to six times control values and LH had increased to twice control values. Estradiol remained at control values for 6 h following surgery but 20alpha-hydroxypreg-4-en-3-one (20alpha-OHP) dropped quickly to baseline values. It is possible that a reduction in circulating 20 alpha-OHP may be responsible for the increases in FSH prior to LH in this group, but the absence of other negative feedback factors from the ovary or adrenal may also be involved.
D004092:20-alpha-Dihydroprogesterone / Q000097:blood; D000311:Adrenal Glands / Q000502:physiology; D000315:Adrenalectomy; D000818:Animals; D002369:Castration; D004958:Estradiol / Q000097:blood*; D004971:Estrus; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006065:Gonadotropins, Pituitary / Q000097:blood*; D007986:Luteinizing Hormone / Q000097:blood; D010053:Ovary / Q000502:physiology; D011247:Pregnancy; D011374:Progesterone / Q000097:blood*; D051381:Rats; D013312:Stress, Physiological / Q000378:metabolism*
D006065:Gonadotropins, Pituitary; D004092:20-alpha-Dihydroprogesterone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone