title: Lymphocyte activation by monovalent fragments of antibodies reactive with cell surface carbohydrates.
abstract: Antibodies reactive with cell surface carbohydrates were isolated from normal chicken serum and were found to be mitogenic for mouse splenic lymphocytes as assayed by both blast transformation and [3H]thymidine incorporation. The Fab' fragments of these carbohydrate-binding immunoglobulins were just as mitogenic as the divalent native antibody. Moreover, succinylated Fab' fragments, which probably would not form self-associating aggregates, showed similar mitogenic properties. All of these results indicate that, at least for saccharide-specific ligands, multipoint attachment and receptor cross-linkage on the cell to which the ligand is attached may not be a stringent requirement for activation.
mesh_terms: D000818:Animals; D001666:Binding Sites, Antibody; D002241:Carbohydrates / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D002645:Chickens; D003429:Cross Reactions; D007140:Immunoglobulin Fab Fragments; D007128:Immunoglobulin Fragments; D008213:Lymphocyte Activation; D051379:Mice; D008934:Mitogens; D013154:Spleen / Q000276:immunology
chemical_list: D002241:Carbohydrates; D007140:Immunoglobulin Fab Fragments; D007128:Immunoglobulin Fragments; D008934:Mitogens