Inactivation of hapten-modified bacteriophage by antibody: increased sensitivity of the assay in media containing polyethylene glycol.
Inactivation of dinitrophenylated bacteriophage T4 by porcine and rabbit anti-dinitrophenyl (DNP) antibodies was studied by the complex inactivation methods. The inactivation titers increased 20-30 times when polyethylene glycol, mol. wt. 6000, was added to the incubation media. Consequently, the detection of dinitrophenylated serum albumin with the modified bacteriophage could be carried out with higher sensitivity upon addition of polyethylene glycol.
D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000032:analysis*; D003090:Coliphages; D003470:Culture Media; D004136:Dinitrobenzenes / Q000276:immunology; D006241:Haptens; D008722:Methods; D011092:Polyethylene Glycols
D000906:Antibodies; D000941:Antigens; D003470:Culture Media; D004136:Dinitrobenzenes; D006241:Haptens; D011092:Polyethylene Glycols