[Separation of reb blood cells in the isopyknic dextran and albumin density gradient].
The separation of red blood cells according to their maturity and age was compared in the isopyknic dextran and albumin density gradients, and the quality of separation was appraised by selected criteria (density distribution curves, accumulation of reticulocytes, activity of G-6-PD). The achieved quality of separation, as measured by the quotient of G-6-PD activity in the cells of lower density/G-6-PD activity in cells of higher density is of equal value in the two media. In the dextran gradient, separation of old cells seems to be accomplished more readily, while albumin affords better separation of young erythrocytes. The reticulocytes are in both media 4-5 mg/ml lighter than the total cell population. When shifting pH from 7.4 to 6.8 both in erythrocytes and reticulocytes the density decreases by 3-6 mg/ml irrespective of the type and pretreatment of the separation medium.
D000418:Albumins; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D002500:Centrifugation, Isopycnic; D003911:Dextrans; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D012156:Reticulocytes / Q000201:enzymology
D000418:Albumins; D003911:Dextrans; D005954:Glucosephosphate Dehydrogenase
Trennungen von roten Blutzellen im isopyknischen Dextran- und Albumindichtegradienten