title: The surface membrane of Leishmania. I. The effects of lectins on different stages of Leishmania braziliensis.
abstract: The infective stages of Leishmania braziliensis, amastigotes and promastigotes subcultured a limited number of times, were agglutinated by Ricinus communis agglutinin and Concanavalin A. These results suggest that terminal ligands similar or identical with alpha-D mannose, alpha-D glucose (specific receptors for Con A), and alpha-D galactose (specific receptor for RCA) are present in the surface membrane of L. braziliensis. Noninfective promastigotes from the same stock, but subcultured approximately 500 times, were not agglutinated by RCA suggesting either the absence of the alpha-D galactose groups in the surface membrane or their presence in a very reduced number. Agglutination with soybean agglutinin, wheat germ agglutinin, or phytohemagglutinin P was not observed in any of the L. braziliensis forms tested. The difference in polysaccharide residues on the surface membrane of L. braziliensis may be related to the different pathogenic properties of the cell.
mesh_terms: D000371:Agglutination; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D006224:Cricetinae; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D007891:Leishmania / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D051379:Mice; D037121:Plant Lectins; D010947:Plants, Toxic; D012278:Ricinus; D013025:Glycine max; D014908:Triticum
chemical_list: D037102:Lectins; D037121:Plant Lectins; D003208:Concanavalin A