title: [The minimal freezing temperature for a necrosis of the epidermis and the influence of cryoprotective agents (author's transl)].
abstract: The sensitiveness to cold of the epidermis (cold threshold) is determined on the auricular skin of 24 rabbits, using freezing temperatures of -10, -15, -20, -25, -30, and -40 degrees C and a freezing time of 30 sec. The experiments are performed with and without application of cryoprotective agents (DMSO, glycerol and aethanol in a 10% solution) before cryosurgery. Only at -20 degrees C a necrosis of the epidermis is seen whereas after application of cryoprotective agents before freezing a necrosis of the epidermis first appears at -30 degrees C. Following these experiments clinically it might be possible to protect the epidermis in the periphery of the freezing area up to a certain degree with the use of cryoprotective agents, so that a topographically more concentrated epidermis-necrosis and therefore a limitation of the cryoeffect to the epidermis to a certain degree could be possible.
mesh_terms: D000818:Animals; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003452:Cryosurgery / Q000379:methods*; D004423:Ear; D009336:Necrosis; D011817:Rabbits; D012871:Skin Diseases / Q000517:prevention & control*; D013696:Temperature
chemical_list: D003451:Cryoprotective Agents
vernacular_title: Die Mindestgefriertemperatur für eine Hautnekrose und ihre Beeinflussung durch Kälteschutzsubstanzen