title: [Characteristics of somatic cell hybrids (mouse X Chinese hamster) with different ratios of parental species chromosome sets. IV. Electrophoretic analysis of several enzymes of the dehydrogenase class].
abstract: Electrophoretic mobilities in polyacrylamide gel of five dehydrogenases: NADP-dependent malate dehydrogenase (NADP-MDH), 6-phosphogluconate dehydrogenase (6PGD), alcohol dehydrogenase (ADH), glucose-6-phosphate dehydrogenase (G6PD) and glutamate dehydrogenase (GDH) were investigated in a series of mouse X Chinese hamster somatic cell hybrids. Seven hybrid lines with different ratio of chromosome sets of hamster and mouse: 1:1, 2:1, 3:1 and 1:2 respectively were studied. NADP-MDH and 6PGD of both parental species and intermediate hybrid bands were present in all hybrids except two lines. These lines had only hamster MDH due to the elimination of mouse chromosomes. A correlation was found between the gene dose and the intensity of the expression of the MDH bands. The mouse type ADH was detected in all hybrids. The hamster ADH was found in one of the hybrid lines that lost all mouse chromosomes during cultivation. It is suggested that hamster ADH activity was suppressed in hybrids by the mouse genome. The species origin of GDH and G6PD could not be established due to similarity of electrophoretic mobilities of respective enzymes in parental cells.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D002875:Chromosomes; D006224:Cricetinae; D005838:Genotype; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006822:Hybrid Cells / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D051379:Mice; D010088:Oxidoreductases / Q000378:metabolism*; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism
chemical_list: D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005969:Glutamate Dehydrogenase
vernacular_title: Kharakteristika gibridov somaticheskikh kletok (mysh' X kitaĭskiĭ khomiachok) s raznym sootnosheniem khromosomnykh naborov roditel'skikh vidov. Soobshchenie IV. Elektroforeticheskiĭ analiz neskol'kikh fermentov klassa degidrogenaz