title: In vitro cultivation and identification of aortic endothelium from miniature pig.
abstract: Pieces of miniature pig aorta were explanted endothelial face down directly on the bottom of Petri dishes. On removal of the aorta fragments after 2-4 days of incubation single cells or small groups of viable endothelium were found attached. The cells developed into a permanent line (at present over 18 passages) and were identified as pure endothelial cells both by light- and electron microscopic examination.
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology* / Q000648:ultrastructure; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D004727:Endothelium / Q000166:cytology; D013552:Swine