[Semisynthetic sheep des-A1-glycine insulin (author's transl)].
The synthetic Des-1-glycine-A-chain of sheep insulin as the monomeric cyclic bisdisulfide and native bovine B-chain bissulfonate were reduced together with mercaptoethanol. They combined at pH 10.6 to yield Des-A1-glycine-insulin. This was purified by gel and ion exchange chromatography. The low insulin activity (0.4 - 0.6%) as measured by the fat cell test as well as the change in the CD spectrum indicated that the loss of the N-terminal glycine of the A-chain results in fully inactive insulin. This confirms the results obtained earlier by partial synthesis of Des-A1-glycine-insulin.
D000818:Animals; D002942:Circular Dichroism; D004220:Disulfides; D005998:Glycine; D007328:Insulin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000302:isolation & purification; D008623:Mercaptoethanol; D011487:Protein Conformation; D012756:Sheep
D004220:Disulfides; D007328:Insulin; D008623:Mercaptoethanol; D005998:Glycine
Semisynthetisches Des-A1-glycin-Schafinsulin