The influence of urea and guanidine chloride on the binding of the binding of the bacterial substrate and inhibitors to hen lysozyme at physiological temperature (40 degrees) (*).
The influence of urea and of guanidine chloride on the binding of the bacterial substrate and of inhibitors such as N-acetylglucosamine or chitotetraose to hen lysozyme were studied at 20 degrees and at 40 degrees C (physiological temperature). The action of urea did not prevent a certain degree of organization of the enzyme compatible with its usual behaviour in the presence of some inhibitors and with its crystallization ; guanidine chloride, already at low concentrations, seemed to have a more severe effect on lysozyme.
D000117:Acetylglucosamine / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D002686:Chitin / Q000494:pharmacology; D003352:Corynebacterium / Q000378:metabolism; D004529:Egg White; D006146:Guanidines / Q000494:pharmacology*; D007700:Kinetics; D009113:Muramidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009844:Oligosaccharides; D013696:Temperature; D013780:Tetroses / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology*
D006146:Guanidines; D009844:Oligosaccharides; D013780:Tetroses; D002686:Chitin; C012238:chitotetrose; D014508:Urea; D009113:Muramidase; D000117:Acetylglucosamine