Automated separation and measurement of lactate dehydrogenase isoenzymes.
We describe a totally automated, computer-controlled system for separating and measuring the activity of lactate dehydrogenase (EC 1.1.1.27) isoenzymes. These isoenzymes in tissue extracts were separated on disposable DEAE-cellulose mini-columns. Resolution was complete except that LD-1 was not resolved from LD-2.
D001322:Autoanalysis / Q000295:instrumentation; D002848:Chromatography, DEAE-Cellulose; D004586:Electrophoresis; D006801:Humans; D009206:Myocardium / Q000201:enzymology; D013154:Spleen / Q000201:enzymology