N-dimethylaminomethylene derivatives for the gas-liquid chromatography of primary sulfonamides.
Dimethylformamide dialkylacetals have been found to react readily with primary sulfonamides to form N-dimethylaminomethylene derivatives. These compounds possess excellent gas-liquid chromatographic properties and can be conveniently prepared at the submicrogram level. Their retention times are much greater than those of other sulfonamide derivatives (e.g., N,N-dimethyl) but their ease of preparation and lack of absorptive properties make them attractive for gas-liquid chromatographic studies. The practical applicability of this derivatization approach to biological studies is illustrated by the gas-liquid chromatographic determination of 3-bromo-5-cyanobenzenesulfonamide in ovine blood using 3,5-dibromobenzenesulfonamide as the internal standard. The method has a detection limit of 25 ppb with electron capture detection.
D000818:Animals; D001969:Bromobenzenes / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods; D004123:Dimethylamines; D012756:Sheep; D013449:Sulfonamides / Q000097:blood*; D014105:Tosyl Compounds; D014297:Trimethylsilyl Compounds
D001969:Bromobenzenes; D004123:Dimethylamines; D013449:Sulfonamides; D014105:Tosyl Compounds; D014297:Trimethylsilyl Compounds