Fatty-acid composition of rat brain lipids. Determined by support-coated open-tubular gas chromatography.
The nonhydroxy fatty acid composition of rat brain lipids (except gangliosides) was determined by support-coated open-tubular (SCOT) gas chromatography. Fatty acids of both odd and even chain lengths ranging from C14 to C26 were detected. Brain lipids contained 49% saturated, 29% monounsaturated, and 22% polyunsaturated fatty acids. Monoenoic fatty acids were mainly of the omega-9 and omega-7 series with minor amounts of omega-10 and amega-11 isomers. Dienes and trienes consisted of omega-6, amega-7, and omega-9 series. Tetraenes were of the omega-6 series. Small amounts of omega-6 and omega-3 pentaenes were detected. The most abundant polyunsaturated fatty acid was 22:6omega-3. The advantages of support-coated open-tubular columns over wall-coated open-tubular columns for the analysis of brain lipid fatty acids are discussed.
D000818:Animals; D001921:Brain; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas / Q000295:instrumentation*; D005026:Ethylene Glycols; D005227:Fatty Acids / Q000032:analysis*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D051381:Rats; D013386:Succinates
D005026:Ethylene Glycols; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D013386:Succinates