Relative activity of alpha-tocopherol and gamma-tocopherol in preventing oxidative red cell hemolysis.
The purpose of this study was to compare the antioxidant activities of alpha-tocopherol and gamma-tocopherol in protecting the red cell membrane against lipid peroxidation. Tocopherols were incorporated into the red cell membrane by incubating cells with solutions of the tocopherols in bovine albumin. The cells were then washed and subjected to the dialuric acid hemolysis test. Analysis of variance of the response curves revealed that gamma-tocopherol had 38% of the activity of alpha-tocopherol. No evidence was found for an interaction between the two tocopherols when present in the red cell membranes simultaneously.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001463:Barbiturates / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects; D004357:Drug Synergism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D050356:Lipid Metabolism; D008297:Male; D010084:Oxidation-Reduction; D010545:Peroxides / Q000378:metabolism; D051381:Rats; D013329:Structure-Activity Relationship; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*; D014811:Vitamin E Deficiency / Q000097:blood
D000975:Antioxidants; D001463:Barbiturates; D010545:Peroxides; D014810:Vitamin E