Studies on the formation of cyclohexylamine and N-methylcyclohexylamine from bromhexine in animals and man, and simultaneous determination of cyclohexylamine and N-methylcyclohexylamine by gas chromatography.
In order to detect cyclohexylamine and N-methylcyclohexylamine simultaneously in urine, a gas chromatographic method was developed in which Chromosorb 103 (porous polymer) was found to be suitable as the column packing. The detection limits were 0.1 mug/ml of cyclohexylamine and 0.4 mug/ml of N-methylcyclohexylamine in urine. In tests on animals administered bromhexine, which contains N-methylcyclohexylamine as part of its side-chain, neither cyclohexylamine nor N-methylcyclohexylamine was detected in the urine.
D000818:Animals; D001964:Bromhexine / Q000378:metabolism*; D002849:Chromatography, Gas; D003514:Cyclohexylamines / Q000652:urine*; D004305:Dose-Response Relationship, Drug; D007267:Injections; D008297:Male; D008745:Methylation; D051381:Rats; D012997:Solvents; D013607:Tablets
D003514:Cyclohexylamines; D012997:Solvents; D013607:Tablets; D001964:Bromhexine