5-methylcytosine in heterochromatic regions of chromosomes in Bovidae.
The centromeric regions of cattle, goat and sheep chromosomes bind anti-5-MeC as revealed by immunofluorescence technique, indicating concentration of 5-MeC at these heterochromatic regions. The centromere of the submetacentric X of cattle remains nearly unstained and so do the centromeres of the acrocentric X chromosomes in goat and sheep. The short arm of the cattle Y exhibits strong anti-5-MeC binding whereas the tiny Y chromosomes of goat and sheep contain no brightly fluorescent material.
D000818:Animals; D005075:Biological Evolution; D002417:Cattle; D002875:Chromosomes / Q000032:analysis; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004276:DNA, Satellite / Q000032:analysis / Q000378:metabolism; D006041:Goats; D006570:Heterochromatin / Q000032:analysis*; D008297:Male; D008745:Methylation; D012730:Sex Chromosomes / Q000032:analysis; D012756:Sheep
D004276:DNA, Satellite; D006570:Heterochromatin; D003596:Cytosine