Properties of sea urchin embryo messenger RNA containing and lacking poly(A).
Various properties of nonhistone messenger RNA species containing poly(A), [+A]mRNA, and lacking poly(A), [-A]mRNA, are described: the rates of turnover of these mRNA classes are not significantly different, as indicated by their similar rates of entry into and decay from the cytoplasm; each mRNA class is essentially entirely transcribed from unique DNA sequences; the ratio of [+A] to [-A] nonhistone mRNA increases with increase in size of free polyribosome, although the average molecular weights of these mRNAs are similar in each polysomal size class. These results indicate that the [+A]mRNA species tend to be more fully loaded with ribosomes than the nonhistone [-A]mRNA species.
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D006657:Histones; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis; D011132:Polyribosomes / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D012617:Sea Urchins / Q000196:embryology; D015053:Zygote / Q000032:analysis*
D006657:Histones; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A