"In vitro" mucin biosynthesis by human gastric mucosa biopsies.
A method is described for the study of the "in vitro" biosynthesis of mucins by human gastric biopsy samples. 14C-glucose was incorporated in the biopsies obtained from the fundus and antral regions of four normal human donors during a four hours incubation period. Labelled glycoproteins (mucins) were released in the medium and were also retained in the tissue-residue. Neutral sugars, fucose, protein and DNA determinations carried out on the tissue homogenates indicated that significant information on mucin biosynthesis can be obtained by this biopsy procedure which is particularly suitable for the exploration of gastric pathology.
D000328:Adult; D001706:Biopsy; D002241:Carbohydrates / Q000032:analysis; D004247:DNA / Q000032:analysis; D005752:Gastric Mucins / Q000096:biosynthesis*; D005753:Gastric Mucosa / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D011506:Proteins / Q000032:analysis
D002241:Carbohydrates; D005752:Gastric Mucins; D011506:Proteins; D004247:DNA; D005947:Glucose