Behaviour of polyclonal human IgE in the course of fractionation.
Normal human pooled plasma was fractionated by a variety of methods. The IgE concentration of the different fractions was determined by a solid-phase radioimmunoassay. The results of these studies indicate, that polyclonal IgE behaves similarly to IgE of myeloma origin. A biospecific method was worked out to purify IgE from fraction III of the cold ethanol fractionation procedure.
D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006801:Humans; D007073:Immunoglobulin E / Q000302:isolation & purification; D009194:Myeloma Proteins / Q000302:isolation & purification; D011092:Polyethylene Glycols / Q000494:pharmacology
D009194:Myeloma Proteins; D007073:Immunoglobulin E; D011092:Polyethylene Glycols