Modification of the epidermal growth factor affecting the stability of its high molecular weight complex.
The epidermal growth factor (EGF) can be isolated from the submaxillary gland of the adult male mouse as part of a high molecular weight complex (HMW-EGF). This complex can be reversibly dissociated into its subunits, EGF AND EGF-binding protein, an arginine esteropeptidase (Taylor, J. M., Cohen, S., and Mitchell, W. M. (1970) Proc. Natl. Acad. Sci. U. S. A. 67, 164-171). The COOH-terminal arginine residue of EGF was quantitatively removed by digestion with carboxypeptidase B...
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D006133:Growth Substances / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008970:Molecular Weight; D010447:Peptide Hydrolases; D010455:Peptides / Q000494:pharmacology; D011485:Protein Binding; D012867:Skin / Q000187:drug effects; D013363:Submandibular Gland / Q000032:analysis
D000596:Amino Acids; D006133:Growth Substances; D046911:Macromolecular Substances; D010455:Peptides; D010447:Peptide Hydrolases