Detection of circulating carcinoembryonic antigen (CEA) by counter-immunoelectrophoresis.
A sensitive method is described for detecting circulating carcinoembryonic antigen (CEA) by combined use of counter-immunoelectrophoresis and intensifiers. Perchloric acid extraction of serum was followed by counter-immunoelectrophoresis against monospecific antiserum to CEA. A precipitin line formed was intensified further by electrophoretic binding of anti-gammaG gamma-globulin and Amido Black staining. Less than 10 ng CEA/ml was detectable. Using this method, a total of 84 sera from patients with or without malignancy was examined. The results obtained were in general agreement with those reported by radioimmunoassay. The method appears to be useful as a diagnostic aid, since it requires neither labeled preparations of purified CEA nor special equipment for assay.
D000951:Antigens, Neoplasm / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D008832:Microchemistry; D009369:Neoplasms / Q000276:immunology
D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen