Preparation and storage of control slides for fetal hemoglobin determination by acid elution.
To simplify the reading of acid eluted blood smears for fetal hemoglobin, positive and negative controls are placed side by side on a single slide. Red blood cells having high Hb F content are stained a bright pink in contrast to cells devoid of Hb F which appear as ghost cells with a faint pink outline. A method for preparing, fixing, and short-term storing of control slides with their subsequent room temperature acid elution and staining is given.
D000143:Acids; D001773:Blood Cells; D005319:Fetal Hemoglobin / Q000032:analysis*; D006452:Hemoglobinometry / Q000295:instrumentation; D006801:Humans
D000143:Acids; D005319:Fetal Hemoglobin