Regional mapping of the human No. 1 and X chromosome in interspecific cell hybrids using an X/1 translocation.
Fibroblasts from a carrier of an X/1 translocation, 46,XY,t(X;1)(q28;q31), were fused with Chinese hamster cells. The resulting hybrids were analyzed for human No. 1 and X-chromosome markers. The data indicate that the loci for PGM1, PGD, PPH, and GuK1 are situated either in the long arm proximal to a break point in band 1q31 or in the short arm. The loci for Pep-C, FH, and GuK1 are located distal to the break point. HPRT and G6PD are probably situated distal to a break point in band q28 of the X chromosome; alpha-Gal A is situated proximal to the break point, either on the long or short arm of the chromosome.
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D002900:Chromosomes, Human, 1-3; D006224:Cricetinae; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology / Q000648:ultrastructure; D012730:Sex Chromosomes; D014178:Translocation, Genetic