Measurement of membrane protein lateral diffusion in single cells.
Fluorescence rapidly returns to spots bleached by a laser beam in the continuous fluorescence of cultured cells labeled on the surface with fluorescein isothiocyanate. The rate of recovery of fluorescence after bleaching can be interpreted as a measure of the lateral diffusion of integral membrane proteins labeled with fluorescein.
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004058:Diffusion; D005456:Fluorescent Dyes; D007834:Lasers; D011506:Proteins / Q000378:metabolism*
D005456:Fluorescent Dyes; D011506:Proteins