Globin synthesis in fibroblasts fused with erythroblasts. I. Avian fibroblasts.
Heterokaryons of chick embryo erythroblasts fused with other avian fibroblasts were studied with regard to globin production. After the incorporation of radioactive amino acids, soluble proteins were separated on SDS-urea polyacrylamide gels. There was a striking increase in radioactivity above background in the globin region from lysates of fusion cultures when compared with fibroblast cultures. This was maximal at 24 hours after fusion, and then declined. Electrophoresis on acid-or alkaline-urea gels further identified the material as globin chains. Tryptic digestion and fingerprinting revealed methionine-labeled peptides characteristic of chick embryo erythroblast globin. An apparent stimulation of globin chain synthesis by heterokaryons compared to erythroblasts was found to be due to a difference in the specific activity of the precursor amino acid pools in the different cell types.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002459:Cell Fusion; D002467:Cell Nucleus; D002642:Chick Embryo; D004372:Ducks; D004794:Enzyme Repression; D004900:Erythroblasts / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005796:Genes; D005914:Globins / Q000096:biosynthesis*; D006454:Hemoglobins / Q000096:biosynthesis; D006822:Hybrid Cells / Q000378:metabolism*; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism
D000596:Amino Acids; D006454:Hemoglobins; D005914:Globins; D007930:Leucine