A comparison between gonadotropin radioimmunoassays with the use of 48 hour and six-day incubation methods.
A double-antibody radioimmunoassay for human follicle-stimulating hormone (FSH) and human luteinizing hormone (LH) which can be carried to completion in 48 hours was compared with the conventional six-day double-antibody radioimmunoassay. The bound-over total (B/T) dose response curves for the 48 hour assay were comparable in slope and dose range with the six-day assay and the four per cent average within-assay and the nine per cent between-assay coefficients of variation for the 48 hour assay were comparable with the variation observed in the conventional six-day assay. Maximum variation among identical serum samples assayed in both systems was 15.3 per cent so that quantitative results obtained with either assay system were comparable.
D006063:Chorionic Gonadotropin / Q000032:analysis; D005640:Follicle Stimulating Hormone / Q000032:analysis*; D006801:Humans; D007457:Iodine Radioisotopes; D007986:Luteinizing Hormone / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*; D013997:Time Factors
D006063:Chorionic Gonadotropin; D007457:Iodine Radioisotopes; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone