[Dynamics of the amino acid composition of the medium during cultivation of isolated organs by the directed perfusion method].
The dynamics of the amino acid composition of the medium under conditions of adequate perfusion of the isolated organs of a dog (sternum, kidney and liver) was studied. It was found that after a 6-hour perfusion of the complex of organs the amount in the perfusion medium of such amino acids as histidine, lysine, alanine, considerably increased, whereas the amount of arginine, serine, aspartic acid, threonine with glutamine, isoleucine, proline, leucine and valine decreased as compared with their initial concentration. The dynamics of the amino acid medium composition during a 4-hour perfusion was studied in experiments with the isolated sternum. The concentration of alanine, lysine and histidine increased in the medium. At the same time there was seen a decrease in the concentration of serine, aspartic acid, isoleucine, tyrosine and phenyl-alanine.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D003470:Culture Media; D004285:Dogs; D009924:Organ Culture Techniques; D009926:Organ Preservation; D010477:Perfusion; D014021:Tissue Preservation
D000596:Amino Acids; D003470:Culture Media
Dinamika aminokislotnogo sostava sredy pri kul'tivirovanii izolirovannykh organov metodom upravliaemoĭ perfuzii