Protein synthesis initiation factors from chicken erythroblasts.
Two factors (IF-I and IF-II) necessary for the initiation of protein synthesis have been partially purified from a 0.5 M KC1 wash of chicken erythroblast polysomes. IF-I mediates the binding of the initiator tRNA and GTP to a 40 S ribosomal subunit, resulting in the formation of a 44 S initiation intermediate. In the presence of IF-II and a suitable RNA template, the 44 S initiation intermediate combines with a 60 S ribosomal subunit to form a functional 80 S initiation complex. The methionyl moiety of the initiator tRNA in the 80 S initiation complex is able to react with puromycin to form methionylpuromycin.
D000818:Animals; D001665:Binding Sites; D002645:Chickens; D004912:Erythrocytes / Q000032:analysis*; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000097:blood; D011132:Polyribosomes / Q000378:metabolism; D011485:Protein Binding; D011691:Puromycin; D012343:RNA, Transfer / Q000378:metabolism; D013698:Templates, Genetic
D010448:Peptide Initiation Factors; D011691:Puromycin; D012343:RNA, Transfer