Applications of gas-liquid chromatography in protein chemistry. Determination of C-terminal sequences on nanomolar amounts of proteins.
A method for the determination of C-terminal amino acids and C-terminal amino acid sequences in nanomolar amounts of proteins is described, based on carboxypeptidase A digestion of the protein, followed by removal of the partially digested protein and quantitative gas-liquid chromatographic determination of the amino acids released after known time intervals. Sequences deduced from the kinetics of release of specific amino acids are compared with the known C-terminal sequences of well-characterized proteins.
D000595:Amino Acid Sequence; D000818:Animals; D002268:Carboxypeptidases / Q000032:analysis; D002417:Cattle; D002849:Chromatography, Gas; D002918:Chymotrypsin / Q000032:analysis; D005934:Glucagon / Q000032:analysis; D007328:Insulin / Q000032:analysis; D007700:Kinetics; D008832:Microchemistry; D009113:Muramidase / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012260:Ribonucleases / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000032:analysis; D014269:Trifluoroacetic Acid
D007328:Insulin; D011506:Proteins; D012710:Serum Albumin, Bovine; D005934:Glucagon; D014269:Trifluoroacetic Acid; D012260:Ribonucleases; D009113:Muramidase; D002268:Carboxypeptidases; D002918:Chymotrypsin