Detection of HBs antigen with latex particles coated with human antibody prepared by affinity chromatography on concanavalin A-sepharose.
Human HBs antibody was isolated by affinity chromatography on HBs antigen absorbed to concanavalin A linked to Sepharose 4B. When a human anti-HBs immunoglobulin preparation obtained by Cohn's cold ethanol fractionation method was used as a starting material, the antibody was concentrated about 10 times in terms of the passive hemagglutination titer with a recovery rate higher than 50%. Latex particles coated with human anti-HBs antibody thus prepared were proved to be useful in detecting HBs antigen in human blood samples. In its sensitivity and in rapidity of its performance, the antibody-coated latex agglutination test seems to be superior to conventional immunodiffusion techniques.
D002846:Chromatography, Affinity; D003208:Concanavalin A; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens / Q000032:analysis*; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods; D012685:Sepharose
D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens; D003208:Concanavalin A; D012685:Sepharose