Carbamycholine prevents giant granule-formation in cultured fibroblasts from beige (Chediak-Higashi) mice.
Primary embryonic fibroblasts isolated from beige (Chediak-Higashi) mice develop pathognomonic giant granules in vitro. Inclusion in the culture medium of carbamylcholine (carbachol) or phorbol myristate acetate (PMA) results in the generation of morphologically normally granules. Chediak-Higashi fibroblasts are highly susceptible to shape changes induced by colchicine. This abnormal property is also corrected by carbachol and PMA.
D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology*; D002478:Cells, Cultured; D002609:Chediak-Higashi Syndrome / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D008247:Lysosomes / Q000187:drug effects* / Q000648:ultrastructure; D010704:Phorbols / Q000494:pharmacology
D010704:Phorbols; D001285:Atropine; D002217:Carbachol