The use of freeze-fracture and negative staining techniques to study the ultrastructure of melanosomes isolated from B16 melanoma.
Freeze-fracture negative staining methods were used to investigate the structure of melanosomes isolated from B16 melanoma. The findings demonstrated the presence of a melanosomal double-layered membrane. Negative staining procedure showed that the melanosomal membrane is sometimes ruptured, displaced or peeled off during the purification process. Fractured melanosomes showed melanin spheres with a mean diameter of 24 nm. This compares with a mean diameter of 26 nm for the melanin spheres revealed by negative staining. The granular appearance of fractured mature melanosomes is due to the fracture following a random path between melanin spheres.
D000818:Animals; D005614:Freeze Fracturing; D008543:Melanins; D008544:Melanocytes / Q000473:pathology / Q000648:ultrastructure*; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology; D009940:Organoids / Q000473:pathology / Q000648:ultrastructure*
D008543:Melanins