Separation of free and protein-bound ligands in the radioimmunoassay by gel filtration-centrifugation.
The application of gel filtration-centrifugation to the separation of free and protein-bound ligands in radioimmunoassay is described. The method is simple and rapid. A large number of samples can be handled simultaneously and the bount radioactivity is directly transferred to the scintillation vial. Using a 9 X 36 mm column of Sephadex G-50 (coarse), 400 mul of the reaction mixture can be separated during 2 min centrifugation.
D000818:Animals; D002498:Centrifugation; D002850:Chromatography, Gel; D004958:Estradiol; D007074:Immunoglobulin G; D008024:Ligands / Q000032:analysis*; D011193:Potassium Iodide; D011485:Protein Binding; D011863:Radioimmunoassay; D013552:Swine
D007074:Immunoglobulin G; D008024:Ligands; D011193:Potassium Iodide; D004958:Estradiol