Increased synthesis of ribonucleic acids in mouse liver after treatment with a nonionic detergent.
A single injection of Tween 40 (polyoxyethylene(20)sorbitan monohexanoate) in the dose range of 600--800 mg/kg body weight induced an increased short-term labeling especially of poly(A)-containing mRNAs in mouse liver (using either [3H]orotic acid or [32P]orthophosphate as RNA precursors), and apparently increased the turnover rates of both rRNAs and mRNAs in this organ over a period of 24 h. In the early period (4 h) after the injection of Tween 40, there was also a significant increase in the content of 32P-labeled adenylic acid in microsomal poly(A)-mRNA fraction. The activity of DNA-dependent RNA polymerases in the nuclei of treated animals was stimulated up to 60% over that in control nuclei in the same period after detergent injection.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011136:Polysorbates / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects*
D011136:Polysorbates; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A; D011092:Polyethylene Glycols; D012321:DNA-Directed RNA Polymerases