title: Urine aldosterone radioimmunoassay: validation of a method without chromatography.
abstract: A simplified radioimmunoassay of urinary aldosterone is reported. Acid-hydrolyzed urine was extracted with dichloromethane and the extract assayed without further purification, Urinary aldosterone values in patients with Cushing's syndrome, low and normal-renin essential hypertension, congenital adrenal hyperplasia, and primary aldosteronism determined by this method agreed closely (r = 0.95, P less than 0.01) with values obtained using a standardized chromatographic method. This simplified assay represents a significant advance in out capabilitites for evaluating patients for abnormalities in aldosterone physiology.
mesh_terms: D000307:Adrenal Gland Diseases / Q000652:urine; D000450:Aldosterone / Q000652:urine*; D002854:Chromatography, Paper; D003480:Cushing Syndrome / Q000652:urine; D006801:Humans; D006929:Hyperaldosteronism / Q000652:urine; D006965:Hyperplasia / Q000652:urine; D006973:Hypertension / Q000652:urine; D011863:Radioimmunoassay / Q000379:methods*
chemical_list: D000450:Aldosterone