Studies on steroids. CIX. O-omega-haloalkyloximes, new derivatives for gas chromatography-mass spectrometry of oxosteroids.
Several kinds of omega-haloalkoxyamines have been prepared as new derivative-forming agents applicable for the gas chromatography-mass spectrometry of carbonyl compounds. Of these reagents, 2-chloroethoxyamine hydrochloride [O-(2-chloroethyl)hydroxylamine] was the most suitable for use with oxosteroids because of its higher reactivity and the appropriate retention value and satisfactory chromatographic properties of the resulting oximes. The contribution to the retention value due to formation of the 2-chloroethyloxime was determined for 5alpha-androstanones having the oxo-group at various positions in the steroid nucleus. As expected, 17-oxosteroid O-2-haloethyloximes exhibit readily distinguishable isotope peaks as a cluster in the mas spectrum.
D015068:17-Ketosteroids / Q000032:analysis*; D000731:Androstanes / Q000032:analysis*; D002849:Chromatography, Gas; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008722:Methods
D015068:17-Ketosteroids; D000731:Androstanes; D007202:Indicators and Reagents