title: Chloral hydrate causes breakdown of polysomes in Chlamydomonas reinhardi in vivo.
abstract: Chloral hydrate produces a biphasic change in the proportion in the cell. Within 1 to 2 min after addition to cells, it inhibits protein synthesis and causes polysomes to break down. The ribosomes dissociate from mRNA by a process which requires protein synthesis but which is apparently abnormal. Released ribosomes do not appear to be bound to fragments of mRNA, but do carry a nascent polypeptide chain. Protein synthesis remains inhibited by more than 85% for over 24 hours, but the apparently normal polyteraction of the cells with chloral hydrate itself and not from its conversion of its usual metabolic products, trichloroethanol or trichloroacetic acid.
mesh_terms: D002696:Chlamydomonas / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D002697:Chloral Hydrate / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D014176:Protein Biosynthesis / Q000187:drug effects; D011691:Puromycin / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D002697:Chloral Hydrate; D011691:Puromycin; D003513:Cycloheximide