The evaluation of various culture media in combination with dimethylsulfoxide for ultrarapid freezing of murine embryos.
Bicarbonate-buffered HTF medium, Medicult, and T6 are as effective as PB1 medium when used in combination with DMSO in ultrarapid freezing of two-cell mouse embryos. However, the use of phosphate-buffered T6 results in reduced in vitro development and inner cell mass size as compared with bicarbonate- and Hepes-buffered T6 when used with 3.5 M of DMSO. Hence, the use of this media for ultrarapid freezing should be avoided when this concentration of DMSO is used.
D000818:Animals; D001639:Bicarbonates; D001755:Blastocyst / Q000502:physiology*; D002021:Buffers; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D004121:Dimethyl Sulfoxide; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010710:Phosphates; D013194:Staining and Labeling
D001639:Bicarbonates; D002021:Buffers; D003470:Culture Media; D010710:Phosphates; D004121:Dimethyl Sulfoxide