H-ras and c-myc RNA expression in human T-cell ALL and in normal human lymphocytes.
We have compared the RNA expression of two oncogenes, H-ras and c-myc, with normal human lymphocytes, normal human T-cells, and human T-cell acute lymphoblastic leukemia (ALL). T-cell ALL RNA was derived from two human T-cell lines (MOLT-3 and CEM) and from five children with T-cell ALL. The RNA expression of H-ras and the third exon of c-myc was determined by northern hybridization. RNA from both MOLT-3 and CEM overexpressed both H-ras and the third exon of c-myc compared to normal lymphocytes and normal T-cells. T-cell ALL samples from four of five children overexpressed H-ras, while three of five patient samples overexpressed the third exon of c-myc when compared to normal lymphocytes and normal T-cells. Additional information on the oncogene expression of T-cell ALL may prove to be useful in the diagnosis and treatment of T-cell ALL.
D015152:Blotting, Northern; D001853:Bone Marrow / Q000473:pathology; D002648:Child; D004587:Electrophoresis, Agar Gel; D005091:Exons; D015870:Gene Expression; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics* / Q000473:pathology; D012313:RNA / Q000032:analysis* / Q000235:genetics; D012334:RNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D012016:Reference Values; D013601:T-Lymphocytes / Q000502:physiology*; D014407:Tumor Cells, Cultured
D012334:RNA, Neoplasm; D012313:RNA