Purification of complement-fixing antigens of Rickettsia sennetsu by ether treatment.
Soluble and particulate complement-fixing antigens of Rickettsia sennetsu were prepared from spleen suspensions of mice infected with the rickettsia and treated with cyclophosphamide. The medium used during purification of antigens, a solution consisting of equal volumes of phosphate-glutamate-sucrose buffer and veronal-buffered saline, was suitable for obtaining antigens with high titers and no anti-complementary activity. By heat treatment, it was demonstrated that the soluble antigen was heat labile and the particulate antigen was heat stable. The soluble antigen was precipitated by 80% saturation with ammonium sulfate. Cross-complement fixation tests using both soluble and particulate antigens revealed that there was no antigenic difference among strains of R. Sennetsu. On the other hand, no cross-activity was observed between R. sennetsu and R. orientalis.
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002021:Buffers; D003168:Complement Fixation Tests; D003429:Cross Reactions; D004986:Ether; D005019:Ethyl Ethers; D006168:Guinea Pigs; D006358:Hot Temperature; D051379:Mice; D012281:Rickettsia / Q000276:immunology*; D012995:Solubility
D000942:Antigens, Bacterial; D002021:Buffers; D005019:Ethyl Ethers; D004986:Ether