Effect ofcholinergic ligands on the lipids of acetylcholine receptor-rich membrane preparations from Torpedo californica.
Ion permeation, triggered by ligand-receptor interaction, is associated with the primary events of membrane depolarization at the neuromuscular junction and synaptic connections. To explore the possible sites of ion permeation, the long-lived fluorescent probe pyrene (fluorescence lifetime approximately 400 nsec) has been inserted into the lipid phase of acetylcholine receptor-rich membrane (AcChR-M) preparations from Torpedo californica. The pyrene probe is susceptible to both fluidity and permeability changes in the lipid bilayer. These changes are detected by variations in the rate of decay of the excited singlet state of pyrene after pulsation with a 10-nsec ruby laser flash. Variations of these lifetimes in the membrane preparations alone or in the presence of quenchers show that binding of cholinergic agonists and antagonists, neurotoxins, and local anesthetics to AcChR-M produces varying effects on the properties of the pyrene probe in the lipid phase. It is concluded that binding of cholinergic ligands to the receptor does not significantly alter the fluidity of permeability of the lipids in the bilayer contact with pyrene. On the other hand, local anesthetics do affect these properties.
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002038:Bungarotoxins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D004557:Electric Organ / Q000378:metabolism*; D005399:Fishes; D007700:Kinetics; D008024:Ligands; D008567:Membranes, Artificial; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011485:Protein Binding; D011721:Pyrenes / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013696:Temperature
D002038:Bungarotoxins; D008024:Ligands; D008567:Membranes, Artificial; D009419:Nerve Tissue Proteins; D011721:Pyrenes; D011950:Receptors, Cholinergic; D000109:Acetylcholine