Expression of vascular cell adhesion molecule-1 in human renal allografts.
The expression of vascular cell adhesion molecule-1 (VCAM-1) in 11 human renal allograft biopsies and 3 normal kidney specimens was investigated by immunocytochemistry. VCAM-1 expression was correlated with the degree of CD3+ T cell infiltration and the clinicopathologic diagnosis of acute rejection. CD3+ infiltrates were seen in all biopsies with rejection, but not in normal biopsies or one with acute tubular necrosis, and were accompanied by CD68+ monocyte/macrophage infiltrates. In normal biopsies, VCAM-1 was present on occasional tubules, where its expression was patchy and restricted to the basolateral surface of cells with slight cytoplasmic staining. The total number of tubules expressing VCAM-1 significantly increased in specimens infiltrated with CD3+ T cells. Moreover, in these infiltrated biopsy specimens, VCAM-1 was present throughout the cytoplasm of tubular cells concentrated on the basolateral surface. VCAM-1 was also observed on vascular endothelial cells where its expression correlated with the degree of CD3+ infiltrate. Mean scores (0 to 3+) for endothelial VCAM-1 expression increased from 0 (CD3+ score, 0) to a mean score of 2.25 in association with CD3+ T cell infiltrates (CD3+ score, 3). Endothelial VCAM-1 was predominantly on vessels in areas of infiltrate, including peritubular capillaries, venules, and arterioles, but was notably absent on glomerular endothelium. VCAM-1 also stained mesangial cells in an occasional CD3+ infiltrated specimen. It was concluded that the expression of VCAM-1 is increased on renal tubules and renovascular endothelium in rejecting renal allografts in association with CD3+ infiltrates.(ABSTRACT TRUNCATED AT 250 WORDS)
D000208:Acute Disease; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis*; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000737:chemistry / Q000473:pathology; D006084:Graft Rejection / Q000473:pathology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D016030:Kidney Transplantation / Q000473:pathology*; D007683:Kidney Tubular Necrosis, Acute / Q000378:metabolism / Q000473:pathology; D007684:Kidney Tubules / Q000737:chemistry / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D009000:Monocytes / Q000473:pathology; D011183:Postoperative Complications / Q000378:metabolism / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000473:pathology; D014184:Transplantation, Homologous; D019010:Vascular Cell Adhesion Molecule-1; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D017252:CD3 Complex; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1; D019010:Vascular Cell Adhesion Molecule-1; C067980:CD68 antigen, human; D000097382:CD68 Molecule