N-acetyl-9-O-L-lactylneuraminic acid, a new acylneuraminic acid from bovine submandibular gland.
The acylneuraminic acid fraction, obtained on mild acid hydrolysis of glycoproteins from bovine submandibular glands, contains approximately 2% N-acety-9-O-L-lactylneuraminic acid. The compound has been isolated and purified by ion-exchange and cellulose column chromatography. The structure has been elucidated using thin-layer chromatography, colorimetry, gas-liquid chromatography/mass spectrometry, periodate oxidation and specific lactate dehydrogenases. An evaluation of the different analytical methods is given.
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D007773:Lactates / Q000032:analysis; D008968:Molecular Conformation; D012794:Sialic Acids / Q000032:analysis*; D013363:Submandibular Gland / Q000032:analysis*
D007773:Lactates; D012794:Sialic Acids; C011624:N-acetyl-9-O-lactylneuraminic acid