Spectrophotometric determination of allylisothiocyanate in mustard seed oil.
Allylisothiocyanate is determined spectrophotometrically after reaction with 2,3-dichloro-1,4-naphthoquinone. For pure samples, the color intensity is proportional to allylisothiocyanate content in the range 0.8-3.0 mg/ml reaction mixture. A modified procedure is used to estimate allylisothiocyanate content of mustard seed oil. The reaction is linear for allylisothiocyanate  concentrations in the range 40-240 mug/ml reaction mixture. Two mustard seed oil samples contained 0.995 not equal to 0.020 and 0.981 not equal to 0.019% allylisothiocyanate.
D009149:Mustard Plant / Q000032:analysis*; D009821:Oils / Q000032:analysis; D010946:Plants, Medicinal; D012639:Seeds / Q000032:analysis; D013053:Spectrophotometry; D013861:Thiocyanates / Q000032:analysis*
D009821:Oils; D013861:Thiocyanates