Spontaneous maturation of oocytes isolated from ovaries of immature hypophysectomized rats.
Oocytes were collected from preantral follicles (200-300 mu in diameter) from 30-day-old immature rats 7 days after hypophysectomy. The ova were cultured in vitro for 17 hrs in a chemically defined medium and scored cytologically for meiotic maturation. Of 534 oocytes that were cultured 89% resumed meiosis; however, 98% of these oocytes arrested in either metaphase or anaphase I. In contrast, 82% of the oocytes isolated from preovulatory follicles (approximately 600 mu in diameter) of adult proestrus rats progressed to metaphase II. These results are discussed in terms of functional FSH and LH receptors on the granulosa cells.
D000818:Animals; D005260:Female; D007016:Hypophysectomy; D066298:In Vitro Techniques; D008540:Meiosis; D006080:Ovarian Follicle / Q000166:cytology; D010060:Ovulation; D010063:Ovum / Q000166:cytology*; D051381:Rats; D012741:Sexual Maturation