Alterations in the transcriptional capacity of hepatic DNA-dependent RNA polymerase I and II by N-hydroxy-2-acetylaminofluorene.
Using hepatic RNA polymerases I and II, acetylaminofluorene modification of DNA in vitro results in reduction of RNA elongation but not in an altered frequency of incorporation of [alpha32P]ribonucleotides. In contrast, in vivo modification of RNA polymerases by a single dose of N-2-hydroxy-2-acetylaminofluorene results in an alteration of their capacity for transcribing a normal RNA product but does not affect the product size transcribed from normal or carcinogen-modified templates.
D000818:Animals; D001483:Base Sequence; D002850:Chromatography, Gel; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005449:Fluorenes / Q000494:pharmacology*; D006881:Hydroxyacetylaminofluorene / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D009691:Nucleic Acid Denaturation; D011089:Polydeoxyribonucleotides / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*
D005449:Fluorenes; D011089:Polydeoxyribonucleotides; D006881:Hydroxyacetylaminofluorene; D004247:DNA; D012321:DNA-Directed RNA Polymerases