Studies on the disposition of cyclophosphamide (NSC-26271) in tumor-bearing mice by means of gas chromatography-chemical ionization-mass fragmentography.
The disposition of cyclophosphamide (CP) in mice bearing Lewis lung tumor has been studied by chemical ionization-mass fragmentography using isophosphamide as the internal standard. The drugs were analyzed as their N-trifluoroacetyl derivatives in order to increase sensitivity and to avoid column adsorption and decomposition phenomena. The minimum detectable amount was 500 pg/injection. Linearity was found up to microgram amounts of the substance without any interference of endogenous compounds.
D000107:Acetylation; D000478:Alkylation; D000818:Animals; D002849:Chromatography, Gas; D003520:Cyclophosphamide / Q000032:analysis* / Q000378:metabolism / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D008175:Lung Neoplasms / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008832:Microchemistry; D009374:Neoplasms, Experimental
D003520:Cyclophosphamide