[The affinity lavage of the abdominal cavity in diffuse peritonitis with liquid sorbents based on cross-linked dextrans].
Experiments were conducted on 35 Wistar rats with generalized purulent peritonitis. Twenty-four hours after infection the authors introduced into the animals' abdominal cavity gels of cross-linked dextrans with immobilized polymixin (50 mg/g dry weight) and terrilithin (100 PE/g dry weight), as well as a combination of these sorbents with 24-hour exposure in a 5 ml volume. The object of the study was the detoxification effect. The authors revealed a phenomenon of agglutination of red cells of an ox or rat sensitized by plasma of sick animals in mixing with sephadex G-200 granules with polymixin W immobilized on them. The agglutination titers determined the activity of the sorbent taking part in lavage of the abdominal cavity. The study showed that cross-linked dextrans with immobilized polymixin B and terrilithin introduced into the abdominal cavity in peritonitis are capable of extracting substrates possessing affinity to them, the polymixin sorbent raises the quality of careful mechanical lavage. Combination of gels based on cross-linked dextrans with enzymatic and affinity functions in relation to toxins leads to detoxification and restoration of the disturbed biochemical values by 87%.
D000005:Abdomen; D000345:Affinity Labels / Q000627:therapeutic use*; D000681:Amylases / Q000627:therapeutic use; D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use; D003432:Cross-Linking Reagents / Q000627:therapeutic use*; D003911:Dextrans / Q000627:therapeutic use*; D004338:Drug Combinations; D005069:Evaluation Studies as Topic; D010447:Peptide Hydrolases / Q000627:therapeutic use; D010538:Peritonitis / Q000097:blood / Q000628:therapy*; D011112:Polymyxin B / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D016060:Sorption Detoxification / Q000379:methods*; D007507:Therapeutic Irrigation / Q000379:methods
D000345:Affinity Labels; D000890:Anti-Infective Agents; D003432:Cross-Linking Reagents; D003911:Dextrans; D004338:Drug Combinations; C009402:amylase, phosphates, proteases drug combinations; D000681:Amylases; D010447:Peptide Hydrolases; D011112:Polymyxin B
Affinnyĭ lavazh briushnoĭ polosti pri razlitom peritonite zhidkimi sorbentami na osnove sshitykh dekstranov.