A comparison of procedures for analysing microsatellite (CA)-repeat polymorphisms.
Dinucleotide repeat sequences ('microsatellites') have been used as polymorphic genetic markers following amplification in the polymerase chain reaction (PCR). We have compared several methods of analysing the PCR products. The most reliable and unambiguous results were obtained when the PCR products were probed with a specific dinucleotide repeat oligonucleotide, so that only the microsatellite-containing products were detectable.
D001943:Breast Neoplasms / Q000737:chemistry; D004247:DNA / Q000097:blood / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D004996:Ethidium; D005819:Genetic Markers; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013194:Staining and Labeling
D004273:DNA, Neoplasm; D005819:Genetic Markers; D015345:Oligonucleotide Probes; D004247:DNA; D004996:Ethidium