Maximum likelihood estimation and identification directly from single-channel recordings.
We present a method for analysis of noisy sampled data from a single-channel patch clamp which bypasses restoration of an idealized quantal signal. We show that, even in the absence of a specific model, the conductance levels and mean dwell times within those levels can be estimated. Estimation of the rate constants of a hypothesized kinetic scheme is more difficult. We present examples in which the rate constants can be effectively estimated and examples in which they cannot.
D000818:Animals; D004553:Electric Conductivity; D007473:Ion Channels / Q000502:physiology*; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological; D011336:Probability; D013997:Time Factors
D007473:Ion Channels