[Sparteine and tedisamil inhibit chloride channels in pulmonary epithelial cells isolated from patients with cystic fibrosis].
Chloride current was measured in cells isolated from the pulmonary epithelium of patients with cystic fibrosis. By applying the whole cell patch clamp method three types of chloride current were identified, i.e. a time-dependent, an activating with time, and an inactivating with time. The time-dependent component of ICl proved to be DIDS sensitive. The effect of the potassium current blocker sparteine and its analogue tedisamil on ICl was tested. both substances were found to be chloride current inhibitors, yet the effectiveness of sparteine was 10(4) times higher.
D001643:Bridged Bicyclo Compounds / Q000494:pharmacology*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism*; D003521:Cyclopropanes / Q000494:pharmacology*; D003550:Cystic Fibrosis / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000187:drug effects*; D008168:Lung / Q000378:metabolism*; D013034:Sparteine / Q000494:pharmacology*
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002712:Chlorides; D003521:Cyclopropanes; D007473:Ion Channels; D013034:Sparteine; C059921:tedisamil
Sparteín a tedisamil inhibujú chlórový prúd v bunkách izolovaných z pl'úcneho epitelu pacientov s cystickou fibrózou.