Affinity partitioning of steroid-binding proteins. The use of polyethylene oxide-bound estradiol for purifying delta 5 goes to 4 3-oxosteroid isomerase.
D001665:Binding Sites; D002846:Chromatography, Affinity; D004958:Estradiol / Q000494:pharmacology; D007535:Isomerases / Q000302:isolation & purification*; D010087:Oxides; D011095:Polyethylenes; D011485:Protein Binding; D011549:Pseudomonas / Q000201:enzymology; D013251:Steroid Isomerases / Q000302:isolation & purification* / Q000378:metabolism
D010087:Oxides; D011095:Polyethylenes; D004958:Estradiol; D007535:Isomerases; D013251:Steroid Isomerases