Entrapment of protein protease inhibitors in red blood cells.
Aprotinin and alpha 1-proteinase inhibitor have been encapsulated in human red blood cells (RBC) by a dialysis technique that involves transient hypotonic haemolysis followed by isotonic resealing. Both protease inhibitors can be encapsulated to a considerable extent. These molecules are released only by haemolysis of the cells and that excludes the possibility of using loaded erythrocytes for a slow release of the inhibitor(s) in the blood stream. However, the stability of the two inhibitors, the evidence for the binding of aprotinin to RBC components, and the results showing inhibition of endogenous proteolytic activity indicate that the inhibitors may be valuable in blocking, at least partially, undesired intraerythrocytic proteolytic reactions.
D007611:Aprotinin / Q000378:metabolism; D003956:Dialysis; D004337:Drug Carriers; D004339:Drug Compounding; D004912:Erythrocytes / Q000737:chemistry; D006461:Hemolysis; D006801:Humans; D011485:Protein Binding; D000515:alpha 1-Antitrypsin / Q000378:metabolism
D004337:Drug Carriers; D000515:alpha 1-Antitrypsin; D007611:Aprotinin