Studies on the isolation and chemical properties of porcine follicle-stimulating hormone.
Porcine follicle-stimulating hormone (pFSH) has been prepared from acetone dried pituitary glands. The acetone powder was initially extracted in pH 5 ethanol-acetate buffer (40% ethanol and acetate at 0.5 ionic strength) containing 0.02% PMSF. The gonadotropin rich fraction, obtained in the 80% ethanol precipitate, was dissolved in 0.12 M NH4HCO3, heated at 60 degrees for 3 min, cooled, and centrifuged. The supernatant was lyophilized for chromatography on QAE-Sephadex, then Sephadex G-100. The pFSH obtained has a biological activity of 15 X NIH-FSH-P-1 and 2.1% sialic acid. The amino acid analysis is characterized by high lysine, aspartic acid, and glutamic acid with notable amounts of threonine, proline, and half-cystine.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D005640:Follicle Stimulating Hormone / Q000032:analysis* / Q000302:isolation & purification; D010902:Pituitary Gland / Q000032:analysis; D013552:Swine
D000596:Amino Acids; D005640:Follicle Stimulating Hormone