Development of a method for the purification of human trypsin by single step affinity chromatography suitable for human isotope incorporation studies.
D000208:Acute Disease; D000681:Amylases / Q000302:isolation & purification / Q000378:metabolism; D015153:Blotting, Western; D002846:Chromatography, Affinity / Q000379:methods*; D004386:Duodenum / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007419:Intestinal Secretions / Q000201:enzymology; D007553:Isotope Labeling; D008970:Molecular Weight; D010195:Pancreatitis / Q000201:enzymology; D014357:Trypsin / Q000302:isolation & purification* / Q000378:metabolism
D000681:Amylases; D014357:Trypsin