Methylation analysis of the major gangliosides of the human alimentary mucosa.
The five major gangliosides of the human alimentary mucosa were purified with silicic acid column chromatography and with thin-layer chromatography. The linkages in the carbohydrate portion were analysed by permethylation with gas chromatography-mass spectrometry. A succesful analysis of the linkages of two hematosides and three tetraglycosylceramides was performed. Two of the tetraglycosylamides had a galactosamine in their chain and one had a glucosamine.
D055598:Chemical Phenomena; D002621:Chemistry; D005688:Galactosamine / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005732:Gangliosides / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006801:Humans; D007413:Intestinal Mucosa / Q000032:analysis*; D013058:Mass Spectrometry; D008745:Methylation; D012794:Sialic Acids / Q000032:analysis
D005732:Gangliosides; D012794:Sialic Acids; D005688:Galactosamine; D005947:Glucose; D005944:Glucosamine; D005690:Galactose