Molecular size and amino acid composition of H-2d antigen solubilized in Nonidet P-40.
H-2d antigenic material solubilized by the detergent Nonidet P-40 from L-1210 mouse leukemia cells was isolated by gel filtration on Bio-Gel P-100. A single peak eluted in the void volume consisted of about 90% protein, 8% hexose and traces of sialic acids. In sedimentation velocity runs, the antigen sedimented as a single peak of 3-1 S. Molecular weight determined by sedimentation equilibrium as well as calculated from amino acid composition was found to be in the range of 53,000 daltons and approx. 45,000-51,000 when calculated from sodium dodecyl sulfate polyacrylamide gel electrophoresis. Secondary structure of H-2d glycoprotein was predicted from the amino acid composition. For NP-40-solubilized H-2d antigen, about 34% of helix, 13% beta sheet and 41% turns was found.
D000596:Amino Acids / Q000032:analysis*; D002460:Cell Line; D003902:Detergents; D006649:Histocompatibility Antigens / Q000032:analysis*; D008970:Molecular Weight; D014461:Ultracentrifugation
D000596:Amino Acids; D003902:Detergents; D006649:Histocompatibility Antigens