Isolation of peptides containing citrulline and the cross-link, epsilon-(gamma-glutamyl)lysine, from hair medulla protein.
The epsilon-(gamma-glutamyl)lysine cross-link has been unequivocally localized in the citrulline-containing protein of hair medulla tissue. This was achieved by the isolation of cross-linked peptides that contain citrulline. The peptides were purified by molecular sieving, cation-exchange and paper chromatography and paper electrophoresis and their amino acid compositions are reported. They were detected by analyzing a large number obtained from a tryptic digest of thermolytic peptides of the medulla protein. There is no evidence at present as to whether the cross-link is in fact inter- or intrachain, or both.
D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D002956:Citrulline / Q000032:analysis; D005971:Glutamates / Q000032:analysis; D006197:Hair / Q000032:analysis; D008239:Lysine / Q000032:analysis; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins; D013820:Thermolysin; D014357:Trypsin
D000596:Amino Acids; D005971:Glutamates; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011506:Proteins; D002956:Citrulline; D014357:Trypsin; D013820:Thermolysin; D008239:Lysine