Isolation and mass spectrometric identification of two metabolites of FK 506 from rat liver microsomal incubation media.
A transient epoxide in equilibrium with its possible isomeric forms, most probably induced easily by neighboring-group assistance of a hydroxy group, and a dihydrodiol formed under the influence of the hydrase enzymic activity and/or by simple hydrolysis were isolated by HPLC and identified by FAB/MS from rat liver microsomal incubation media.
D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016339:Spectrometry, Mass, Fast Atom Bombardment; D016559:Tacrolimus / Q000032:analysis / Q000378:metabolism*
D016559:Tacrolimus