Organic cation uptake in vitro by the rabbit iris-ciliary body, renal cortex, and choroid plexus.
The uptake in vitro of radioactively labeled test substances was studied in tissues from albino rabbits. Choroid plexus, slices of outer renal cortex, and iris-ciliary body were incubated in a K-rich medium containing one of the cations 14C-Emepronium (Cetiprin), 14C-tetraethylammonium, 14C-choline, or 125I-o-iodobenzyltrimethylammonium and sometimes the anions 131I-o-iodohippurate and 125I-iodipamide. Choroid plexus and renal cortex accumulated all test substances, some to very high tissue-medium ratios. The iris-ciliary body preparation accumulated the anions well but the organic cations only weakly. The only convincing uptake was that of Emepronium. The affinity of this uptake system seemed to be similar to that in the kidney, half-saturating around 10(-4)M Emepronium.
D000818:Animals; D001692:Biological Transport; D001949:Bretylium Compounds / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D002831:Choroid Plexus / Q000378:metabolism*; D002924:Ciliary Body / Q000378:metabolism*; D004629:Emepronium / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007458:Iodipamide / Q000378:metabolism; D007465:Iodohippuric Acid / Q000378:metabolism; D007498:Iris / Q000378:metabolism*; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D013329:Structure-Activity Relationship; D013757:Tetraethylammonium Compounds / Q000378:metabolism
D001949:Bretylium Compounds; D007457:Iodine Radioisotopes; D013757:Tetraethylammonium Compounds; D004629:Emepronium; D007465:Iodohippuric Acid; D002794:Choline; D007458:Iodipamide