ET-1 released histamine from guinea pig pulmonary but not peritoneal mast cells.
Endothelin(ET)-1 triggered histamine release of mast cells from pulmonary tissue but not from the peritoneal cavity of guinea pigs. The observed difference in response to ET-1 was attributable to a quantitative difference in ET-1 binding sites between both cells. The concentrations of ET-1 required for half maximal release of histamine and half maximal binding of [125I]ET-1 were approximately 0.05 and 0.08 nM, respectively. The release of histamine by ET-1 was a Ca(2+)-dependent but not a cytotoxic process. These observations, taken together, suggest that ET-1 induces histamine release from mast cells in a receptor-dependent fashion.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D016232:Endothelins / Q000378:metabolism / Q000494:pharmacology*; D006168:Guinea Pigs; D006636:Histamine Release / Q000187:drug effects*; D007700:Kinetics; D008168:Lung / Q000502:physiology*; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D010529:Peritoneal Cavity / Q000502:physiology; D017466:Receptors, Endothelin / Q000378:metabolism; D013004:Somatostatin / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D016232:Endothelins; D017466:Receptors, Endothelin; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D013004:Somatostatin