The cytoprotective properties of prostaglandin E2 against the toxic effects of actinomycin C on embryonic neural retina cells.
Prostaglandins (PGs) have been shown to cytoprotect various tissue types against the toxic effects of many chemicals. The mechanism of this protection is poorly understood, but the involvement of cAMP is often implied. Only one previous study examined nervous tissue and PG protection. The present study was designed to determine if PGE2 affords cytoprotection to a more specific nervous tissue (embryonic neural retina) from the toxicity of actinomycin C (AMC) using a trypan blue exclusion assay. The lowest concentration of PGE2 (2 x 10(-5)M) had no effect, but as the concentration increased (3 x 10(-5)M and 5 x 10(-5)M), PGE2 did afford protection against AMC in a dose dependent fashion. Theophylline treated cells were not protected, suggesting that cAMP may not be the primary mechanism of protection.
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D000242:Cyclic AMP / Q000502:physiology*; D003609:Dactinomycin / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000633:toxicity; D015232:Dinoprostone / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D012160:Retina / Q000166:cytology / Q000187:drug effects* / Q000196:embryology
C030013:cactinomycin; D003609:Dactinomycin; D000242:Cyclic AMP; D015232:Dinoprostone