The effective synthesis of uridylyl/3'-5'/5-methylcytidylyl/3'-5'/guanosine.
The triester method was adapted to the synthesis of uridylyl/3'-5'/5-methylcytidylyl/3'-5'/guanosine. As the protecting groups 4-methoxy-5,6-dihydro-2H-pyran for 2'-OH and 5'-OH groups of uridine and 2'-OH group of 5-methylcytidine, methoxymethylidene for I:3'-cis-diol system of guanosine, and benzoyl for the amino groups of 5-methylcytidine and guanosine were used. The obtained product was characterised by UV, electrophoresis, chromatography, an enzymatic digestion and alkaline hydrolysis.
D003562:Cytidine; D006151:Guanosine; D009682:Magnetic Resonance Spectroscopy; D008722:Methods; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014529:Uridine
D009841:Oligonucleotides; D009843:Oligoribonucleotides; D006151:Guanosine; D003562:Cytidine; D014529:Uridine