A simple method for hemoglobin measurement in cell culture system.
A simple method of hemoglobin analysis in a cell culture system is described. Hemoglobins synthesized in cell cultures are labeled with radioactive amino acids. The cell extract containing radiolabeled hemoglobin is mixed with A, F, S, C, hemoglobin markers and separated by cellulose acetate electrophoresis. Individual bands of hemoglobin are cut from the gel and analyzed for radioactivity. This method is especially useful for determination of newly synthesized minute amount of hemoglobin in cell extracts that are difficult to visualize by staining procedure.
D000755:Anemia, Sickle Cell / Q000097:blood / Q000473:pathology*; D000818:Animals; D002478:Cells, Cultured; D004588:Electrophoresis, Cellulose Acetate; D015672:Erythroid Precursor Cells / Q000737:chemistry*; D005319:Fetal Hemoglobin / Q000032:analysis; D005622:Friend murine leukemia virus; D006427:Hemin / Q000494:pharmacology; D006441:Hemoglobin A / Q000032:analysis; D006451:Hemoglobin, Sickle / Q000032:analysis; D006452:Hemoglobinometry / Q000379:methods*; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D007930:Leucine; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis; D014411:Neoplastic Stem Cells / Q000737:chemistry* / Q000187:drug effects; D014316:Tritium
D006451:Hemoglobin, Sickle; D006454:Hemoglobins; D009363:Neoplasm Proteins; D014316:Tritium; D006427:Hemin; D006441:Hemoglobin A; D005319:Fetal Hemoglobin; D007930:Leucine