[Structural rearrangements in membranes induced by anesthetics].
Studies were carried out on the effects of anesthetics on the stability of membranes to sodium dodecylsulfate and trypsin. The initial rate of membrane desintegration by detergent evaluated by light scattering with stopped flow method was increased in the presence of ethanol, propanol, benzyl alcohol, procain-amide (erthrocyte membrane) and chlorpromazine (rat brain synaptosomes). Concentrations of anesthetics which correspond to half of the maximum effect were very close to that of blocking the action potential and inducing 50%--antihemolysis. Alcohols and procaine at anesthetic concentrations inhibited proteolysis of isolated erythrocyte membranes but were without effect on their ultrasonic fragments. Increase of membrane desintegtation rate is interpreted as structural rearrangements of membranes with the weakening of detergent sensitive intermolecular interactions. Proteolysis inhibition of ghosts but not the gragments indicates against the participation of the basic func of proteins in the structural membrane rearrangements.
D000433:1-Propanol / Q000494:pharmacology; D000438:Alcohols / Q000494:pharmacology*; D000818:Animals; D001592:Benzyl Alcohols / Q000494:pharmacology; D002417:Cattle; D002746:Chlorpromazine / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects*; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008566:Membranes / Q000187:drug effects; D011342:Procainamide / Q000494:pharmacology*; D051381:Rats; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013574:Synaptosomes / Q000187:drug effects*; D014357:Trypsin / Q000494:pharmacology
D000438:Alcohols; D001592:Benzyl Alcohols; D012967:Sodium Dodecyl Sulfate; D000431:Ethanol; D000433:1-Propanol; D014357:Trypsin; D011342:Procainamide; D002746:Chlorpromazine
Strukturnye perestroĭki membran, indutsiruemye anestetikami