[Laboratory diagnosis of Pneumocystis carinii: comparison of cytological, immunofluorescent and immunocytochemical staining].
Authors review several methods for diagnosis of Pneumocystis carinii on BAL fluid in AIDS patients aiming to identify ideal routine technic. Giemsa, Gomori and Toluidine Blue staining. Direct and Indirect Immunofluorescence. Immunocytochemical methods were tested and advantages/disadvantages compared on BAL fluid from AIDS patients. Pneumocystis carinii was detected in 7 out 21 cases (33%). Gomori staining and Toluidine Blue staining were chosen for routine Pneumocystis carinii detection while IF technics are deserved as very useful in fields showing high fungal ++ contamination (inducted sputum).
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001399:Azure Stains; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D011010:Pneumocystis / Q000276:immunology / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology; D013194:Staining and Labeling; D014048:Tolonium Chloride
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001399:Azure Stains; D014048:Tolonium Chloride
Diagnosi di laboratorio di Pneumocystis carinii: paragone tra colorazioni citologiche, immunofluorescenza e immunocitochimica.