Release and depletion of substance P by capsaicin in substantia gelatinosa studied with the antibody microprobe technique and immunohistochemistry.
Using an antibody microprobe technique, we have detected substance P release from the region of the substantia gelatinosa of the cat during the first, but not the second, 30 min of topical application of capsaicin (1-3%) to the tibial nerve. Immunohistochemical analysis also showed that substance P-like immunoreactivity was markedly reduced in the superficial layer of the dorsal horn 30 min after application of capsaicin. These results indicate that substance P is released and then depleted from primary afferent central terminals following acute application of capsaicin to the peripheral sensory nerve.
D000344:Afferent Pathways / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002211:Capsaicin / Q000494:pharmacology*; D002415:Cats; D007150:Immunohistochemistry; D008839:Microelectrodes; D009411:Nerve Endings / Q000187:drug effects* / Q000378:metabolism; D013373:Substance P / Q000378:metabolism*; D013376:Substantia Gelatinosa / Q000187:drug effects* / Q000378:metabolism; D014741:Video Recording
D000906:Antibodies; D013373:Substance P; D002211:Capsaicin