title: Temperature-dependent template switching during in vitro cDNA synthesis by the AMV-reverse transcriptase.
abstract: Reverse transcriptase template switching has been invoked to explain several aspects of retroviral replication and recombination, and has been reported in vitro for the Moloney murine leukemia virus (M-MuLV) reverse transcriptase. During in vitro cDNA synthesis, the avian myeloblastosis virus (AMV) reverse transcriptase can switch from one template to another in a homology-dependent and temperature-dependent manner. Chimeric cDNA molecules are generated within 30 min at high incubation temperatures, with an increasing efficiency from 42 degrees C to 50 degrees C. Such products are detectable only after much longer incubation times when primer extension reactions are carried out at lower temperatures (90 min at 37 degrees C).
mesh_terms: D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis*; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D013698:Templates, Genetic; D014157:Transcription Factors / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; C067996:MotA protein, Enterobacteria phage T4; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D014157:Transcription Factors; D014764:Viral Proteins; D004247:DNA; D012194:RNA-Directed DNA Polymerase