Activity staining of endoglucanases in polyacrylamide gels.
The endoglucanases of Penicillium funiculosum were analyzed for the presence of multiple forms using a modified version of the Congo red method. Postelectrophoretic slab gels were directly incubated in a solution of carboxymethylcellulose for a period as short as 15 min and then the activities were visualized by staining with Congo red. Ten distinct bands of clearances were obtained indicating the presence of at least as many multiple forms.
D000180:Acrylic Resins; D002480:Cellulase / Q000032:analysis*; D003224:Congo Red; D005656:Fungal Proteins / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis*; D010407:Penicillium / Q000201:enzymology*; D011489:Protein Denaturation; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods
D000180:Acrylic Resins; D005656:Fungal Proteins; D007527:Isoenzymes; C016680:polyacrylamide gels; D003224:Congo Red; D002480:Cellulase