Base composition heterogeneity of mammalian DNAs in CsCl-netropsin density gradient.
DNAs of 15 mammals and some lower organisms were analysed by CsCl-netropsin density gradient centrifugation. Increased resolving power of this method enabled to detect many new components in mammalian DNAs. Distinct components were detected in the density range of the main band. These components found in different mammalian DNAs have probably limited variation in the G+C content. Most of other components seems to be species specific. The DNAs of lower organisms form homogeneous band even in the presence of netropsin. The relation between densities in CsCl-netropsin and CsCl density gradient is nonlinear. This result supports a hypothesis that in high ionic strength netropsin is preferentially bound to (dA.dT) clusters.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004247:DNA; D003854:Deoxyribonucleotides / Q000032:analysis; D008099:Liver / Q000032:analysis; D008970:Molecular Weight; D013045:Species Specificity
D003854:Deoxyribonucleotides; D004247:DNA