title: Expression of DRG during murine embryonic development.
abstract: We had previously characterised a cDNA which encodes a novel GTP-binding protein DRG. The expression of drg gene is down-regulated during the embryonic development of murine central nervous system. Further analysis of drg mRNA and protein in adult mouse tissues and various cell lines of different origins indicated that it is expressed widely, albeit at low and variable levels. In situ hybridisation analysis of mRNA expression in sections of mouse embryos indicated that drg is expressed strongly in various embryonic tissues. The expression of drg mRNA is greatly reduced in newborn animals. At cellular level, DRG protein can be detected in the cytoplasm. These observations suggest that DRG may play multiple roles in development and normal cell metabolism.
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins / Q000032:analysis / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D015151:Immunoblotting; D017403:In Situ Hybridization; D051379:Mice; D009928:Organ Specificity; D016716:PC12 Cells; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; C078418:developmentally regulated GTP-binding protein; D011061:Poly A; D012313:RNA; D019204:GTP-Binding Proteins