Thin layer chromatography of parathion as paraoxon with cholinesterase inhibition detection.
A simple, sensitive, and rapid method is described for the quantitative estimation of ng amounts of parathion (O,O-diethyl O-p-nitrophenyl phosphorothioate) as paraoxon (O,O-diethyl O-p-nitrophenyl phosphate) on thin layer chromatograms. Paraoxon is detected by inhibition, using p-nitrobenzenediazonium fluoroborate as the chromogenic reagent. This chromogenic reagent is more sensitive than Fast Blue B or indoxyl acetate; 0.1 ng may be detected and amounts from 5 to 50 ng may be estimated. The method is a viable alternative to gas chromatographic analysis for parathion.
D000818:Animals; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D008099:Liver / Q000201:enzymology; D008832:Microchemistry; D010261:Paraoxon / Q000032:analysis*; D010278:Parathion / Q000032:analysis*; D051381:Rats
D002800:Cholinesterase Inhibitors; D010278:Parathion; D010261:Paraoxon