Anaerobic coryneforms isolated from human bone marrow and skin. Chemical, biochemical and serological studies and some of their biological activities.
Eighteen isolates if anaerobic coryneforms from human bone marrow and skin and four type strains of Propionibacterium were studied chemically, biochemically and antigenically. All of the isolates were identified as Propionibacterium acnes; of the 18 isolates,16 belonged to sterotype I and two to serotype II. By means of gas liquid chromatography and mass spectral analysis, a large amount of iso-type fatty acids, such as iso-pentadecanoic and iso-heptadecanoic acids were detected in whole cells of isolates and type strains. Antitumor and adjuvant effects of the isolates and type strains were found to differ considerably among the strains. One of the isolates, P. acnes C-7, which showed potent biological activities was fractionated by hot phenol-water extraction. The resulting insoluble middle layer was found the most effective in tumor protection, adjuvant action in immune response and phagocytic activity in mice.
D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D000818:Animals; D001853:Bone Marrow / Q000382:microbiology*; D002458:Cell Fractionation; D003429:Cross Reactions; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D051379:Mice; D011424:Propionibacterium / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D012510:Sarcoma 180 / Q000628:therapy; D012867:Skin / Q000382:microbiology*
D000276:Adjuvants, Immunologic; D005227:Fatty Acids