Demonstration of transganglionically transported choleragenoid in rat spinal cord by immunofluorescence cytochemistry.
Injections of the B-subunit of cholera toxin (CTB) were made into the sciatic nerve of the rat. Following a survival of 2-3 days, the fluorescent antibody technique was used to show that CTB can be utilized as a highly sensitive immunocytochemically detectable transganglionic tracer for primary afferent fibers in the spinal cord. CTB-labeled fibers as well as fibers containing calcitonin gene-related peptide- (CGRP-) or substance P-like immunoreactivity were visualized simultaneously by using different fluorochromes. However, no double labeled fibers were found.
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002772:Cholera Toxin; D005260:Female; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D005724:Ganglia / Q000033:anatomy & histology*; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000033:anatomy & histology* / Q000166:cytology; D013373:Substance P / Q000378:metabolism
D005456:Fluorescent Dyes; D013373:Substance P; D002772:Cholera Toxin; D006735:Horseradish Peroxidase; D015740:Calcitonin Gene-Related Peptide