Catabolism of cholesterol by bovine adrenal-cortex enzymes: in vitro formation of oxygenated sterols and side-chain cleavage products.
The identification of sterols and steroids formed by incubation of [7alph-3H, 26-14C]cholesterol with mitochondrial enzymes from bovine adrenal cortex is reported. Only 17% of the radioactivity associated with cholesterol metabolites was found in pregnenolone, whereas 15% was reliable to oxygenated sterols and 6% to steroid compounds. The significance of the formation of these compounds is discussed particularly as regards oxygenated cholesterol derivatives.
D000302:Adrenal Cortex / Q000201:enzymology*; D000311:Adrenal Glands / Q000201:enzymology*; D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000378:metabolism*; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D008928:Mitochondria / Q000201:enzymology; D011284:Pregnenolone / Q000096:biosynthesis; D013261:Sterols / Q000378:metabolism
D013261:Sterols; D011284:Pregnenolone; D002784:Cholesterol