Expression of the gene coding for the light chain of calpactin I (annexin II) in cell lines DiFi, HT-29, and WI-38.
Calpactin I (annexin II) light chain gene messages were expressed in the DiFi and HT-29 human colon cancer cell lines, as well as in the diploid lung fibroblast cell line WI-38. However, expression of an approximately 1.0 kb transcript was stronger in DiFi and HT-29 cells than in WI-38 cells. The moderate to strong expression of such transcripts in DiFi and HT-29 cells indicates that the calcium binding protein, calpactin I, may be abundant in colon carcinoma cells. Calpactin I is the major substrate of pp60v-src, a tyrosine-specific protein kinase encoded by v-src, whose cellular homologue c-src also codes for a tyrosine kinase (pp60c-src), known to be activated in colon carcinomas and in cell lines derived from them (including HT-29). Abundance of calpactin I in such cells is consistent with the possibility that activation of the pp60c-src tyrosine kinase contributes to the origin of human colon cancers.
D017302:Annexins / Q000235:genetics*; D015152:Blotting, Northern; D002460:Cell Line; D003110:Colonic Neoplasms; D015342:DNA Probes; D015870:Gene Expression; D006801:Humans; D008168:Lung; D008565:Membrane Proteins / Q000235:genetics*; D008970:Molecular Weight; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017302:Annexins; D015342:DNA Probes; D008565:Membrane Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012313:RNA