[Determination of creatine kinase-MB in serum using inhibiting antibodies (author's transl)].
A new method for the determination of creatine kinase-MB activity in the serum is presented. The principle of this method is the direct measurement of the activity of creatine kinase M subunits by inhibiting antibodies. The total test procedure takes 15 min. In the sera of all the 83 patients tested, who have clinically proven myocard infarction, creatine kinase-MB activity can be measured between the 6th and 28th hour after infarction. At the time of maximum total creatine kinase activity the percentage of creatine kinase-MB activity is between 6 and 17%, the mean value being 8%. In cases of emergency this method can be used for the differential diagnosis of elevated total creatine kinase activities of unknown origin.
D004796:Clinical Enzyme Tests / Q000379:methods; D003402:Creatine Kinase / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007527:Isoenzymes / Q000097:blood*; D009203:Myocardial Infarction / Q000175:diagnosis; D009206:Myocardium / Q000201:enzymology*
D007527:Isoenzymes; D003402:Creatine Kinase
Bestimmung der Aktivität von Creatinkinase MB im Serum unter Verwendung inhibierender Antikörper