title: Changes in plasma membrane structure associated with malignant transformation in human urinary bladder epithelium.
abstract: Integral membrane proteins are visualized as intramembrane particles (IMP; also called membrane-associated particles) at the cleaved surfaces of freeze-fractured plasma membranes. Topographical distributions of the IMP of urothelial cell membranes in normal human bladder and for a small series of low-grade noninvasive transitional cell carcinomas and invasive transtional cell carcinomas are shown to be significantly different. Using several statistical methods that test IMP topography via à vis the random (Poisson) hypothesis, it is demonstrated that IMP are mildly aggregated in plasma membranes of normal human urothelial cells and that, in noninvasive carcinomas, IMP aggregation is increased. In invasive transitional cell carcinomas, IMP are statistically nonaggregated and are in a random distribution in the plane of the membrane. IMP numerical densities are also altered in the course of neoplastic transformation. IMP are significantly increased in number in plasma membranes in human noninvasive transitional cell carcinomas but are similar to control values in invasive tumors. Loss of IMP and changes in IMP topography may be related to tumor invasiveness or they may represent an epiphenomenon.
mesh_terms: D002295:Carcinoma, Transitional Cell / Q000473:pathology; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic; D005614:Freeze Fracturing; D006801:Humans; D008854:Microscopy, Electron; D009363:Neoplasm Proteins / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D009363:Neoplasm Proteins