Isolation and sequence of sheep immunoglobulin E heavy-chain complementary DNA.
A complementary DNA (cDNA) corresponding to sheep immunoglobulin E heavy (IgE H) chain messenger RNA (mRNA) was isolated and sequenced. A fragment of sheep IgE constant H (epsilon)-chain was initially synthesized using the polymerase chain reaction (PCR) and single-stranded cDNA prepared from the mRNA of parasite-stimulated sheep lymph nodes. This fragment was then used to probe a cDNA library, again prepared from a parasite-stimulated lymph node. A recombinant clone containing cDNA encoding a sheep IgE H-chain of 1802 base pairs was isolated and the H-chain cDNA-sequenced. The nucleotide sequence was found to code for a complete sheep IgE H-chain, consisting of both variable (V) and constant (C) regions. When the amino acid sequence derived from the nucleotide sequence was compared to other species epsilon-chains, interesting homologies and differences between corresponding domains were found, including conservation in cysteine and tryptophan residues and variable glycosylation sites.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics*; D007139:Immunoglobulin epsilon-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000235:genetics / Q000276:immunology*
D007139:Immunoglobulin epsilon-Chains; D012333:RNA, Messenger; D011061:Poly A; D007073:Immunoglobulin E; D012313:RNA; D004247:DNA