title: Synapse-specific expression of acetylcholine receptor genes and their products at original synaptic sites in rat soleus muscle fibres regenerating in the absence of innervation.
abstract: To test the hypothesis that synaptic basal lamina can induce synapse-specific expression of acetylcholine receptor (AChR) genes, we examined the levels mRNA for the alpha- and epsilon-subunits of the AChR in regenerating rat soleus muscles up to 17 days of regeneration. Following destruction of all muscle fibres and their nuclei by exposure to venom of the Australian tiger snake, new fibres regenerated within the original basal lamina sheaths. Northern blots showed that original mRNA was lost during degeneration. Early in regeneration, both alpha- and epsilon-subunit mRNAs were present throughout the muscle fibres but in situ hybridization showed them to be concentrated primarily at original synaptic sites, even when the nerve was absent during regeneration. A similar concentration was seen in denervated regenerating muscles kept active by electrical stimulation and in muscles frozen 41-44 hours after venom injection to destroy all cells in the synaptic region of the muscle. Acetylcholine-gated ion channels with properties similar to those at normal neuromuscular junctions were concentrated at original synaptic sites on denervated stimulated muscles. Taken together, these findings provide strong evidence that factors that induce the synapse-specific expression of AChR genes are stably bound to synaptic basal lamina.
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000502:physiology; D015870:Gene Expression / Q000502:physiology*; D017403:In Situ Hybridization; D009132:Muscles / Q000502:physiology*; D009469:Neuromuscular Junction / Q000502:physiology*; D012313:RNA / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000235:genetics; D012038:Regeneration / Q000235:genetics*; D013569:Synapses / Q000502:physiology*
chemical_list: D011950:Receptors, Cholinergic; D012313:RNA