Cholesteryl sulfate and sterol sulfatase in the human reproductive tract.
Cholesteryl sulfate is a component of human seminal plasma (avg. 445 mug%) and spermatozoa (15 mug/10 (9) cells) and represents more than 85% of the sterol sulfate fraction. This conjugate is avidly  bound by spermatozoa when compared to other steroids or steroid sulfates. Autoradiographic localization of CS associated with the spermatozoa revealed a greater accumulation of the radioactivity in the acrosomal region in many, but not all, of those cells examined. Semen is not a site of metabolism of the sterol sulfate but the enzyme, sterol sulfatase, is present in the human female reproductive tract. This cleavage enzyme was detected in Graafian follicles and the activity in the endometrium was ten-fold that found in the Fallopian tube. These findings lead to the proposal that cholesteryl sulfate, an amphipathic molecule ideally suited for interaction with membrane components and implicated in erythrocyte membrane stabilization, may be involved in membrane modifications of the spermatozoa during the process of fertilization.
D002582:Cervix Mucus / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D002788:Cholesterol Esters; D003897:Desmosterol / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009215:Myometrium / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012661:Semen / Q000201:enzymology / Q000378:metabolism*; D013094:Spermatozoa / Q000201:enzymology / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism; D013261:Sterols / Q000378:metabolism; D013429:Sulfatases / Q000378:metabolism*
D002788:Cholesterol Esters; D011506:Proteins; D013256:Steroids; D013261:Sterols; D003897:Desmosterol; D002784:Cholesterol; D013429:Sulfatases; C007045:cholesteryl sulfate