High resistance to cucumber mosaic virus conferred by satellite RNA and coat protein in transgenic commercial tobacco cultivar G-140.
A chimeric vector was constructed to express cucumber mosaic virus (CMV) satellite (Sat) RNA and coat protein (CP). Transgenic lines of tobacco cultivar G-140 expressing CP and Sat-RNA were obtained; these lines had high resistance to CMV. Fifty to 70% of the transgenic plants were symptomless 90 days after inoculation with 25-50 micrograms/ml of CMV. Resistance was about twice that conferred by the Sat-RNA or the CP gene alone in transformed plants.
D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D002678:Chimera; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded; D005814:Genes, Viral; D005822:Genetic Vectors; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000502:physiology*; D010935:Plant Diseases; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012313:RNA / Q000235:genetics*; D019244:RNA, Satellite; D012367:RNA, Viral / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D014170:Transformation, Genetic
D004277:DNA, Single-Stranded; D019244:RNA, Satellite; D012367:RNA, Viral; D012313:RNA