The reliability of a mechanized procedure (Perkin-Elmer C4) for the Enzymatic determination of uric acid according to Kageyama.
The enzymatic determination of the uric acie concentration in urine and serum according to Kageyama ((1971), Clin. Chim. Acta 31, 421--426), which excludes the deproteinization of samples, was adapted to the C4 automatic analyzer (Perkin-Elmer). The reliability of this procedure and its correlation with an UV-method were investigated. The interaction from-sample-to-sample was considerable, but this could be reduced by the addition of Brij-35. The recovery of uric acid added to protein-containing samples (about 96%) was better than with the UV-method. Novaminsulfone was the only substance tested which interfered significantly.
D001322:Autoanalysis / Q000379:methods; D001798:Blood Proteins; D002374:Catalase; D003951:Diagnostic Errors; D004177:Dipyrone / Q000097:blood; D004355:Drug Stability; D006801:Humans; D007202:Indicators and Reagents; D014021:Tissue Preservation; D014503:Urate Oxidase; D014527:Uric Acid / Q000032:analysis*
D001798:Blood Proteins; D007202:Indicators and Reagents; D014527:Uric Acid; D004177:Dipyrone; D002374:Catalase; D014503:Urate Oxidase