Molecular cloning of the carboxy terminus of a canine tracheobronchial mucin.
A cDNA library constructed from canine tracheal mRNA was screened with polyclonal antiserum specific to canine tracheal apomucin (CTM-A). Eight antibody reactive clones were isolated and purified to clonality. One of the clones, designated pCTM-A, had a 1.7 kb insert and included a single open reading frame with a poly (A)+ tail. The amino acid composition of the encoded protein was consistent with that expected for CTM-A. The fusion protein produced by cloning the 1.7 kb insert in the pMALc expression vector reacted with the purified anti-apomucin CTM-A antibody. Also, polyclonal antibodies raised to the purified protein product encoded by pCTM-A reacted with deglycosylated CTM-A confirming that this clone does indeed code for apomucin CTM-A. This is the first report of a cDNA encoding the C-terminus of a canine tracheal mucin.
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004285:Dogs; D015723:Gene Library; D008969:Molecular Sequence Data; D009077:Mucins / Q000032:analysis / Q000235:genetics*; D004676:Myelin Basic Protein / Q000032:analysis / Q000235:genetics; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014132:Trachea / Q000502:physiology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D009077:Mucins; D004676:Myelin Basic Protein; D010455:Peptides; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D004247:DNA