The paradoxical effect of fatty acid on steroid-albumin interaction.
Careful investigation of the influence of palmitic and lauric acid on the interaction of progesterone and testosterone with several batches of untreated and defatted bovine and human serum albumins have revealed that, by contrast with published data for studies with progesterone as well as nonsteroid ligands, there is a surprising stimulation rather than inhibition of binding, albeit with a reduction of the apparent number of binding sites in almost all instances. Furthermore, fatty acid tends to minimize or eliminate the well-known differences in affinity between bovine and human albumin for interactions with these two steroids. The values for binding affinity in the interaction of testosterone with these batches of human serum albumin are significantly higher than those previously published by us and other authors and the value for progesterone-bovine albumin interaction is not in accordance with the "polarity rule". Studies of these same interactions by ultraviolet difference spectroscopy give further evidence of the augmentation in binding but, in the case of defatted bovine albumin only, the aromatic difference troughs are indicative of tyrosine perturbation whereas refatted bovine albumin, defatted and refatted human albumin manifest tryptophan perturbation. Quantitative correlation of perturbation with level of bound steroid suggests that fatty acid alters the ratio (possibly hydrogen-bonded to non hydrogen-bonded) of two forms of bound steroid. There is also further evidence that the binding sites for testosterone and progesterone are not identical.
D001665:Binding Sites; D006801:Humans; D007700:Kinetics; D007850:Lauric Acids; D010169:Palmitic Acids; D011374:Progesterone; D011485:Protein Binding; D011487:Protein Conformation; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D013056:Spectrophotometry, Ultraviolet; D013739:Testosterone
D007850:Lauric Acids; D010169:Palmitic Acids; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D013739:Testosterone; D011374:Progesterone