Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose.
The growth of Chinese hamster somatic cells was inhibited by 0.2 mg/cc of 2-deoxygalactose. Mutants partially or fully resistant to 2-deoxygalactose were isolated in a single-step or two-step selection. Some of them did not grow as well as the wild type; one of them which lacked galactokinase(EC.2.7.1.6) activity did not grow at all in galactose medium. The galactokinase kinetic properties (Vmax & kmax of the other mutants and of the wild type were different. Therefore resistance resulted either from the possible absence of galactokinase synthesis or from a structural mutation, possible a missence mutation, in the galactokinase gene.- A simple diagnostic test for juvenile cataract is proposed.
D002460:Cell Line; D004351:Drug Resistance; D005643:Fucose / Q000494:pharmacology*; D005690:Galactose / Q000378:metabolism*; D009154:Mutation; D010770:Phosphotransferases / Q000378:metabolism*
D005643:Fucose; D010770:Phosphotransferases; D005690:Galactose