Bone cells in culture: morphologic transformation by hormones.
Hormones and purine nucleosides and nucleotides induced cultured bone cells to transform transiently from a spherical to a stellate shape. Cytochalasin B also induced the transformation. The change was blocked by colchicine and vinblastine, but not by lumicolchicine or cycloheximide. This morphologic transformation may provide a dynamic model of hormone action and bone cell modulation in vitro.
D000818:Animals; D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003470:Culture Media; D004837:Epinephrine / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D010281:Parathyroid Hormone / Q000494:pharmacology; D011684:Purine Nucleosides / Q000494:pharmacology*; D011685:Purine Nucleotides / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors; D014747:Vinblastine / Q000494:pharmacology
D003470:Culture Media; D006728:Hormones; D010281:Parathyroid Hormone; D011684:Purine Nucleosides; D011685:Purine Nucleotides; D014747:Vinblastine; D003078:Colchicine; D004837:Epinephrine