Purification of staphylococcal alpha-toxin by adsorption chromatography on glass.
Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chromatography, is composed of a single electrophoretic form, containing less than 2% of other forms.
D000327:Adsorption; D000818:Animals; D000838:Anions; D002845:Chromatography / Q000379:methods; D005898:Glass; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D011817:Rabbits; D013211:Staphylococcus aureus; D014118:Toxins, Biological / Q000302:isolation & purification*
D000838:Anions; D014118:Toxins, Biological