Selection of biochemically variant clones of human lymphoid cells.
Two established clones of human lymphoid cells, Raji and P3HRI, could be cloned in semi-solid agarose-containing medium at high efficiency. Several other human lymphoid cell lines examined cloned poorly if at all under these conditions. Variant clones of Raji and P3HRI resistant to 6-thioguanine, and in the case of Raji also to either 8-azaadenine or 5-bromo-2'-deoxyuridine, were selected and characterized.
D001372:Aza Compounds / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D003470:Culture Media; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008967:Molecular Biology; D011687:Purines / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D012641:Selection, Genetic; D013866:Thioguanine / Q000494:pharmacology
D001372:Aza Compounds; D003470:Culture Media; D011687:Purines; D011743:Pyrimidines; D013866:Thioguanine; D001973:Bromodeoxyuridine