Giant sarcoplasmic reticulum vesicles: a study of membrane morphogenesis.
Rabbit sarcoplasmic reticulum vesicles were fused into giant proteoliposomes in a medium of 0.1 M KCl, 10 mM Tris-maleate, pH 7.0, 10 micrograms ml-1 antipain, 10 micrograms ml-1 leupeptin, 25 IU per ml Trasylol, 3 mM NaN3, 3.75% PEG 1500 and 3% DMSO by brief exposure to 37 degrees C, followed by incubation for 4 h at 25 degrees C. Approximately 5-10% of the sarcoplasmic reticulum elements underwent fusion, forming single-walled spherical vesicles of 1-25 microns diameter, in which the polarity of the native membrane was preserved. The Ca(2+)-stimulated ATPase activity remained essentially unchanged after fusion. On exposure to decavanadate in a Ca(2+)-free medium the spherical vesicles assumed a corrugated appearance with the formation of long ridges separated by deep furrows that eventually pinched off longitudinally and separated into numerous long crystalline tubules of uniform (approximately 0.1 microns) diameter. The vanadate-induced transformation of giant vesicles into tubules implies that the geometry of the sarcoplasmic reticulum membrane is determined by the conformation of the Ca(2+)-ATPase.
D000818:Animals; D000976:Antipain / Q000494:pharmacology; D007611:Aprotinin / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000502:physiology*; D003460:Crystallization; D004533:Egtazic Acid / Q000494:pharmacology; D007811:Lanthanum / Q000494:pharmacology; D007976:Leupeptins / Q000494:pharmacology; D008081:Liposomes; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000502:physiology*; D008854:Microscopy, Electron; D009024:Morphogenesis; D009124:Muscle Proteins / Q000502:physiology*; D011510:Proteolipids; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology / Q000648:ultrastructure*; D013696:Temperature; D014638:Vanadates / Q000494:pharmacology
D007976:Leupeptins; D008081:Liposomes; D008565:Membrane Proteins; D009124:Muscle Proteins; D011510:Proteolipids; C020485:proteoliposomes; C028521:lanthanum chloride; D000976:Antipain; D014638:Vanadates; D004533:Egtazic Acid; D007811:Lanthanum; D007611:Aprotinin; D000252:Calcium-Transporting ATPases; C032854:leupeptin