The glycoprotease of Pasteurella haemolytica A1 eliminates binding of myeloid cells to P-selectin but not to E-selectin.
HL-60 cells and neutrophils treated with the glycoprotease from Pasteurella haemolytica A1, an enzyme which is specific for O-sialoglycoproteins, were found to be incapable of binding P-selectin but still bound E-selectin. Comparative analysis of [35-S] cysteine labeled proteins from HL-60 cells by 2-dimensional electrophoresis indicated that two major proteins with M(r) 100 and 115 kd were significantly removed from cells which had been treated.
D015703:Antigens, CD / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism; D019040:E-Selectin; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D017493:Leukocyte Common Antigens / Q000378:metabolism; D051917:Leukosialin; D016978:Mannheimia haemolytica / Q000201:enzymology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000378:metabolism* / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D011485:Protein Binding; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D051917:Leukosialin; D008562:Membrane Glycoproteins; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D016201:Receptors, Lymphocyte Homing; C465014:SPN protein, human; D012795:Sialoglycoproteins; D017493:Leukocyte Common Antigens; D008666:Metalloendopeptidases; C070738:O-sialoglycoprotein endopeptidase; D003545:Cysteine