Simple determination of 3alpha-hydroxy fraction and 3beta-hydroxy fraction of urinary 17-ketosteroids.
We describe a method for determination of the 3alpha-hydroxysteroid fraction and 3beta-hydroxysteroid fraction of 17-ketosteroids in urine. The method includes enzymatic hydrolysis with beta-glucuronidase and extraction with ethyl acetate and color development of 3beta-hydroxysteroids by 3beta-hydroxysteroid oxidase and of total 17-ketosteroids by the Zimmermann reaction. The value for 3alpha-hydroxysteroids is calculated by subtracting the value for 3beta-hydroxysteroids from the value for total 17-ketosteroids. Recovery was assessed and results compared with those by an accepted method. Results were satisfactory for measuring these fractions of 17-ketosteroids.
D015068:17-Ketosteroids / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods; D005966:Glucuronidase; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases; D006914:Hydroxysteroids / Q000652:urine*; D008722:Methods; D008832:Microchemistry
D015068:17-Ketosteroids; D006914:Hydroxysteroids; D006913:Hydroxysteroid Dehydrogenases; D005966:Glucuronidase