Influenza of age on chromatin transcription in murine tissues using a heterologous and an homologous RNA polymerase.
No gross changes in the compositions of chromatin prepared from liver, kidney and brain were noted with age. A decreased age-dependent in vitro chromatin template activity was observed using an Escherichia coli RNA polymerase. This was paralleled by a reduction in the number of initiation sites on the chromatin for the enzyme. However, using a homologous RNA polymerase definite age-related specificities in template activity were found. When chromatin and enzyme were 'age-matched', maximal activity was observed. This levle of activity was comparable in both 6 and 31-month-old mice, which suggested no impairment of function with age.
D000375:Aging; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D013045:Species Specificity; D014158:Transcription, Genetic
D002843:Chromatin; D012321:DNA-Directed RNA Polymerases