[A two-step determination of plasma total cholesterol and free cholesterol by gas-liquid chromatography. Suggestion for a reference method (author's transl)].
A technique for the determination of free and esterified plasma cholesterol by gas-liquid chromatography is proposed as a reference method. After a critical examination of all the stages to prove its validity, the method is compared to the usual techniques. Such a comparison confirms that colorimetric methods are more accurate after a lipidic extraction and with a purification step by liquid-solid chromatography.
D002784:Cholesterol / Q000031:analogs & derivatives* / Q000097:blood*; D002788:Cholesterol Esters / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D003124:Colorimetry / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans
D002788:Cholesterol Esters; D002784:Cholesterol
Dosage en deux temps du cholestérol total et du cholestérol libre plasmatiques par chromatographie gas-liquide. Proposition d'une methode de référence