[Heterogeneous Epstein-Barr virus infection patterns in peripheral T cell lymphoma of the angioimmunoblastic lymphadenopathy type].
32 cases of T cell lymphoma of angioimmunoblastic lymphadenopathy type (AILD-TCL) were investigated for their association with Epstein-Barr virus (EBV). Polymerase chain reaction (PCR) for detection of EBV-DNA and in situ hybridization for EBV-encoded small nuclear RNAs (EBER) produced almost identical results, showing that all but one of AILD-TCL cases contained EBV genomes. Three distinctive patterns of EBV infection were observed after immunophenotypical characterization of EBER positive cells: 1. predominant infection of B-immunoblasts (26% of the cases), 2. predominant infection of neoplastic T cells (42% of the cases) and 3. infection of few small lymphocytes (32% of the cases). EBV-encoded latent membrane protein was frequently detectable in cases exhibiting patterns 1 and 2. These findings suggest that, in AILD-TCL patients B cells and especially T cells are highly susceptible to a persistent EBV infection which may lead to a growth advantage of infected cells.
D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000145:classification / Q000382:microbiology* / Q000473:pathology*; D017403:In Situ Hybridization; D016411:Lymphoma, T-Cell, Peripheral / Q000145:classification / Q000382:microbiology* / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012342:RNA, Small Nuclear / Q000032:analysis / Q000235:genetics
D004279:DNA, Viral; D012342:RNA, Small Nuclear
Heterogene Epstein-Barr Virus Infektionsmuster beim peripheren T-Zellenlymphom vom Typ der angioimmunoblastischen Lymphadenopathie.