A specific radioimmunoassay for PGE2 using an antibody with high specificity and a sephadex LH-20 microcolumn for the separation of prostaglandins.
Antiserum against PGE2 was raised in rabbits following immunization with prostaglandin-hen-gamma-globulin conjugate. The antiserum exhibited 14% cross reactivity with PGE1 and far less cross-reaction with heterologous prostaglandins. A microcolumn of Sephadex LH-20 was used for a partial, but sufficient separation of PGE2 from PGE1 and a complete separation from heterologous prostaglandins to ensure a specific RIA for PGE2. The precision of the method in the rage 10-500 picograms showed a coefficient of variation varying between 4 and 13%. The detection limit was 10 picograms corresponding to 15 pg/ml of PGE2 in serum. In order to demonstrate the validity of the method values obtained for non-diuretic rat renal venous serum were compared with those obtained using the isotope derivative method of Bojesen & Buckhave (1972) on the same samples. The concentrations of PGE2 obtained were 239 +/- 25 pg/ml and 250 +/- 58 pg/ml, respectively.
D000818:Animals; D000918:Antibody Specificity; D002850:Chromatography, Gel / Q000379:methods*; D007106:Immune Sera; D007114:Immunization; D008297:Male; D011454:Prostaglandins A / Q000097:blood / Q000302:isolation & purification; D011458:Prostaglandins E / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D011460:Prostaglandins F / Q000097:blood / Q000302:isolation & purification; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats
D007106:Immune Sera; D011454:Prostaglandins A; D011458:Prostaglandins E; D011460:Prostaglandins F