Zonal rotor analysis of the subcellular localization of alpha-glycerphosphate dehydrogenase, alpha-naphthyl palmitate and beta-naphthyl laurate hydrolases in the mucosa of the guinea-pig small intestine.
Whole homogenate of guinea-pig small intestine mucosa was analysed by centrifugation in a zonal rotor. The results indicate that FAD-linked alpha-glycerophosphate dehydrogenase is localized in the mitochondria and that NAD-linked alpha-glycerophosphate dehydrogeanse is a soluble phase enzyme. An enzyme hydrolysing alpha-naphthyl palmitate at an acid pH was localized in the lysosomes and was activated by 0.1% Triton X-100 and by freezing and thawing. An alkaline hydrolase acting on beta-naphthyl laurate was localized in the 'microsomes'. The possibility of this enzyme being different from alpha-naphthyl acetate hydrolase is discussed.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002501:Centrifugation, Zonal; D004950:Esterases / Q000378:metabolism*; D005260:Female; D005615:Freezing; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006168:Guinea Pigs; D007421:Intestine, Small / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D011092:Polyethylene Glycols; D013347:Subcellular Fractions / Q000201:enzymology
D011092:Polyethylene Glycols; D005993:Glycerolphosphate Dehydrogenase; D004950:Esterases