Interactions of antisera, sera, and oral fluid with glucosyltransferases.
Partially purified glucosyltransferases (GTF) isolated from Streptococcus mutans OMZ 176 and respective rabbit antisera were used to study enzyme-antibody interactions. A comparison between sensitive serological techniques and a functional inhibition test based on a radioenzyme assay demonstrated that the latter test system was the only one that discriminated between different antisera. Positive reactions in high dilutions in the former test systems were explained by the involvement of non-GTF contaminants and/or antibodies against enzyme regions distant to the  catalytic site. The minute cross-reactions between two enzyme fractions and the respective antisera in the functional inhibition test indicated that the two immunogens contained mainly GTF that differed in the structure of their catalytic region. Control rabbit sera, rat oral fluid, and insoluble and soluble glucans considerably activated the GTF eluted with a 0.5 M phosphate buffer from hydroxapatite. It is suggested that these enzymes had additional binding sites for macromolecules inherent to rabbit sera and rat oral fluid, respectively, and that the observed increase in enzyme activity was due to a more stable enzyme conformation. Possibly the stimulation of GTF by the soluble glucan fraction was caused by a primer and/or acceptor function; however, this was not the case of the insoluble glucan. A stable complex was formed in the absence of the enzyme substrate, sucrose, the activity of which was not readily enhanced. It is concluded that the GTF of strain OMZ 176 are composed of multiple, multi-reactive molecules that enable these enzymes to act as cross-linking agents.
D000042:Absorption; D000818:Animals; D000906:Antibodies; D001826:Body Fluids / Q000276:immunology*; D003168:Complement Fixation Tests; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism; D006386:Hemagglutination Tests; D006461:Hemolysis; D007106:Immune Sera / Q000494:pharmacology; D009061:Mouth Mucosa / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D013291:Streptococcus / Q000276:immunology*; D013295:Streptococcus mutans / Q000201:enzymology / Q000276:immunology*
D000906:Antibodies; D007106:Immune Sera; D005964:Glucosyltransferases