A rapid specific radioimmunoassay for unconjugated estriol in plasma.
A rapid, non-chromatographic radioimmunossaay for unconjugated estriol in pregnancy plasma has been developed which utilizes a commonly available antiestrogen antisera. Estradiol-17beta and estrone demonstrate 135% relative cross-reactivity with our antiserum, as compared with 100% for estriol. Specificity is achieved by purification of estriol with solvent partitioning using benzene: petroleum ether (1:1). The results obtained using this method are similar to a radioimmunoassay utilizing a highly specific, but commercially unavailable, antiestriol antiserum. The method is precise, with coefficients of variation ranging from 3.0 to 8.2%.
D000918:Antibody Specificity; D004964:Estriol / Q000097:blood* / Q000302:isolation & purification; D004967:Estrogens; D005260:Female; D006801:Humans; D011247:Pregnancy; D011863:Radioimmunoassay
D004967:Estrogens; D004964:Estriol