Interpretation of the properties of chromatin extracts from mammalian nuclei.
Chromatin from standard preparations of nuclei stabilized by magnesium ions at 0-4 degrees C was degraded during the nuclear isolation, and newly-synthesized chromatin was degraded slightly more slowly than the ;older' chromatin. The significance of this observation, and its relation to the interpretation of the properties of nucleoprotein extracts is discussed.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis* / Q000378:metabolism; D002843:Chromatin / Q000302:isolation & purification* / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration; D008274:Magnesium / Q000494:pharmacology; D008837:Micrococcus / Q000201:enzymology; D008970:Molecular Weight; D051381:Rats
D002843:Chromatin; D004720:Endonucleases; D008274:Magnesium; D002118:Calcium