An optimal rice-based oral rehydration solution: the effect of different concentrations of amylase on production of short chain polymers of glucose.
As part of a plan to develop a rice-based oral rehydration solution containing short polymers of glucose instead of glucose, we determined the concentration of amylase that would yield the largest amount of short chain polymers. Thai rice (25 g) was boiled with 500 ml of distilled water for 30 min. Of 200 ml supernatant rice water obtained, 100 ml were digested with different amounts of amylase after cooling to 50 degrees C for 60 min, boiled for 5 min, and centrifuged (10,000 g, 25 degrees C) for 60 min. The resulting supernatant (80 ml) was freeze-dried; 1.75 g of the powder obtained were dissolved in 3.5 ml of water, passed through a Bio-Gel P2 column to separate short chain polymers (2-9 molecules of glucose) and long chain polymers (> 9 molecules of glucose), which were identified by spectrophotometry (lambda = 190 nm) or by high performance liquid chromatography. Ten mg of amylase (equivalent to 12,000 modified Wohlgemath units) per 100 ml of rice water was optimal for the production of short polymers of glucose from rice.
D000681:Amylases / Q000494:pharmacology*; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D012275:Oryza / Q000737:chemistry*; D011108:Polymers / Q000378:metabolism; D015532:Rehydration Solutions / Q000737:chemistry* / Q000378:metabolism
D011108:Polymers; D015532:Rehydration Solutions; D000681:Amylases; D005947:Glucose