title: One-tube versus two-step amplification of RNA transcripts using polymerase chain reaction.
mesh_terms: D000818:Animals; D001483:Base Sequence; D004274:DNA, Recombinant / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase; D004586:Electrophoresis / Q000379:methods; D006321:Heart / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000502:physiology*; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D017208:Rats, Wistar; D019914:Taq Polymerase; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D012313:RNA; D019914:Taq Polymerase; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase