In vitro system for the synthesis of teichoic acid linked to peptidoglycan.
A crude cell wall preparation from Staphylococcus aureus H prepared by the method of Mirelman and Sharon (1972) was shown to catalyze the synthesis of polyribitol phosphate linked to the cell wall peptidoglycan. The reaction used cytidine diphosphate (CDP)-ribitol as a substrate and in addition required the presence of CDP-glycerol, uridine diphosphate (UDP)-N-acetyl-D-glucosamine, and adenosine triphosphate. Incubation of radioactive CDP-glycerol with the crude cell wall preparation resulted in the transfer of glycerol phosphate residues to the cell wall; this reaction was greatly stimulated by the presence of UDP-N-acetylglucosamine. These data suggest that polyribitol phosphate is linked to the cell wall peptidoglycan by an oligomer contaning N-acetyl-D-glucosamine and glycerol phosphate.
D000255:Adenosine Triphosphate / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002474:Cell-Free System; D005994:Glycerophosphates / Q000378:metabolism; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism; D010457:Peptidoglycan / Q000096:biosynthesis; D013211:Staphylococcus aureus / Q000378:metabolism*; D013682:Teichoic Acids / Q000096:biosynthesis*; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism
D005994:Glycerophosphates; D009702:Nucleoside Diphosphate Sugars; D010457:Peptidoglycan; D013682:Teichoic Acids; D014537:Uridine Diphosphate N-Acetylglucosamine; D000255:Adenosine Triphosphate