title: CD5 and immunoglobulin VH gene expression in B-cell lines from patients with autoimmune diseases.
abstract: We studied the CD5 mRNA expression and VH gene family usage in Epstein-Barr virus (EBV)-immortalized B-cell lines derived from the blood of patients with type 1 diabetes (IDDM) of recent onset and of patients with polyneuritis cranialis multiplex (cranial neuritis; CN). After immortalization with EBV, at least 10 cell lines from each subject were tested for surface CD5 and CD20. mRNA expression was studied using cDNA probes for the six VH families as well as for CD5. The EBV lines from the IDDM patients used the VHIV family more frequently and VHI and VHII families less frequently than lines from controls. EBV lines from CN patients expressed the VHI and VHII families more often than those of the controls. When the IDDM and CN lines were compared, the lines derived from IDDM patients were found to use VH families I and II less frequently and VH families IV and V more frequently than lines from CN patients. There were no significant differences in the mean numbers of CD5+ B cells in the cell lines tested. More than half of the lines from each patient expressed CD5 at the mRNA level. No correlation was seen between the expression of surface CD5 and the level of CD5 mRNA expression. There was, however, a positive correlation between the usage of VH families III, V and VI, and the CD5 mRNA expression. In conclusion, the usage of VH families I to VI seemed to differ in patients with IDDM and CN. No differences were seen in the surface CD5 expression, but the lines expressing CD5 mRNA preferentially used the VH families III, V and VI.
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis*; D001323:Autoantibodies / Q000032:analysis; D001327:Autoimmune Diseases / Q000276:immunology*; D001402:B-Lymphocytes / Q000378:metabolism*; D018956:CD5 Antigens; D002460:Cell Line; D002472:Cell Transformation, Viral; D002648:Child; D003391:Cranial Nerves / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology; D005260:Female; D005803:Genes, Immunoglobulin / Q000502:physiology*; D006801:Humans; D015151:Immunoblotting; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis*; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis*; D008297:Male; D009443:Neuritis / Q000276:immunology; D011061:Poly A / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger
chemical_list: D015703:Antigens, CD; D001323:Autoantibodies; D018956:CD5 Antigens; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA