A potency assay for brucella allergens in guinea pigs.
In the development of a biological potency assay for brucella allergens by skin testing of guinea pigs, it was observed that the delayed hypersensitivity reactions to a given dose depended upon (1) the method of sensitization of the guinea pigs, (2) the time interval between sensitization and skin test and (3) the total of allergen given per animal. Data illustrating these factors are presented and a method for titrating allergens is recommended.
D000485:Allergens / Q000032:analysis*; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000276:immunology; D002002:Brucella / Q000276:immunology*; D006168:Guinea Pigs / Q000276:immunology; D006968:Hypersensitivity, Delayed
D000485:Allergens; D000942:Antigens, Bacterial; D001426:Bacterial Proteins