In vitro cytotoxicity studies in bladder and renal cell cancer.
Attempts to obtain cell lines from bladder and renal cancer patients were done in 42 patients, with success to obtain primary cultures in 25 and long-term cultures in 7. Cell-mediated and humoral immunity was demonstrated in both tumors by microcytotoxicity in vitro. No cytotoxicity could be deomonstrated against target cells obtained from metastatic cancer tissue. Abrogation of cell-mediated immunity by serum factors was demonstrated. No recurrence was obtained in the group of patients showing positive lymphocytotoxicity during follow-up.
D000912:Antibodies, Neoplasm / Q000032:analysis; D000937:Antigen-Antibody Reactions; D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D007680:Kidney Neoplasms / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D000912:Antibodies, Neoplasm; D003165:Complement System Proteins