Protective effect of zinc on heme biosynthesis disturbances in rabbits after administration per os of tin.
In the present study the lowest p.o. doses of tin affecting heme biosynthesis in rabbits were determined and the protective effect of zinc on these disorders was evaluated. The experiment was performed on female rabbits who received per os single doses of SnCl2 x 2 H2O (10, 100, and 200 mg Sn/kg) and ZnSO4 (50 mg Zn/kg s.c.). The activities of delta-aminolevulinic acid dehydratase (ALA-D) in the whole blood, free erythrocyte propoporhyrins, urine delta-aminolevulinic acid, and coproporphyrins (CP-U) were determined. In animals administered tin at a dose of 100 mg Sn/kg, ALA-D activity decreased by about 80% and two- to threefold increases in the ALA and CP concentrations in urine were observed. A protective effect of zinc with respect to ALA-D activity was noticed in both groups (100 and 200 mg Sn/kg) after combined administration of both metals. Results of an interaction between zinc and tin were also observed to reduce ALA levels in urine, whereas zinc did not protect against an effect of tin on CP excretion.
D000284:Administration, Oral; D000622:Aminolevulinic Acid / Q000652:urine*; D000818:Animals; D003306:Coproporphyrins / Q000652:urine*; D004347:Drug Interactions; D005260:Female; D006418:Heme / Q000096:biosynthesis*; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D011817:Rabbits; D014001:Tin / Q000008:administration & dosage*; D017971:Tin Compounds; D015032:Zinc / Q000008:administration & dosage* / Q000494:pharmacology
D003306:Coproporphyrins; D017971:Tin Compounds; C023599:stannous chloride; D006418:Heme; D014001:Tin; D000622:Aminolevulinic Acid; D000623:Porphobilinogen Synthase; D015032:Zinc