title: The kinetics of cholesterol oxidase synthesis by Nocardia rhodocrous.
abstract: The production of cholesterol oxidase by 3 liter batch cultures of Nocardia rhodocrous growing on a glycerol/yeast extract medium was investigated. Cholesterol was shown to be a good inducer of the enzyme. The optimum time for cholesterol addition and the quantity to be added were determined, resulting in a 15-fold yield increase. Cholesterol oxidase synthesis was influenced by the dissolved oxygen tension. Maximum cholesterol oxidase production was obtained at 30-40% air saturation. The effect of growth conditions on the extraction of cholesterol oxidase by Triton X-100 was investigated. The scale-up of the fermentation to 800 liters in a pilot-plant fermenter is described.
mesh_terms: D000730:Androstadienes / Q000032:analysis; D000735:Androstenedione / Q000032:analysis; D000736:Androstenes / Q000032:analysis; D002783:Cholestenones / Q000032:analysis; D002784:Cholesterol / Q000032:analysis; D004790:Enzyme Induction; D005285:Fermentation; D006913:Hydroxysteroid Dehydrogenases / Q000096:biosynthesis* / Q000378:metabolism; D007664:Ketosteroids / Q000032:analysis; D007700:Kinetics; D009615:Nocardia / Q000201:enzymology* / Q000254:growth & development; D010100:Oxygen / Q000494:pharmacology; D010313:Partial Pressure
chemical_list: D000730:Androstadienes; D000736:Androstenes; D002783:Cholestenones; D007664:Ketosteroids; D000735:Androstenedione; D002784:Cholesterol; D006913:Hydroxysteroid Dehydrogenases; D010100:Oxygen