[Analysis of the long-term results of contact of human cell cultures with influenza virus].
Influenza A/Hong Kong/1/68 and A/Victoria/35/72 viruses may produce in primarily trypsinized human embryo kidney (HEK) cell culture a chronic form of persistence accompanied by periodic emergence of cytopathic changes arrested by additional medium change. This persistence causes morphological changes in HEK culture manifested in the substitution of epithelial elements by fibroblasts and prolongs the "life" of culture by over 100 days as compared to the controls. The chronic form of persistence of these viruses in human embryo lung diploid cell culture (HELDC) was accompanied by a cytoproliferogenic effect but did not lead to histomorphological changes and did not exert "oncogenic-lide" changes in cell membranes. Inapparent infection with A/Hong Kong/1/68 virus in HELDC did not cause any histomorphological and cytokaryological abnormalities and was accompanied by complete elimination of the virus from the culture. No oncornavirus contamination was found in HELDC cluture either in intact state or after inoculation with influenza virus.
D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D066298:In Vitro Techniques; D009975:Orthomyxoviridae; D013997:Time Factors
Analiz otdalennykh posledstviĭ kontakta kletok kul'tur chelovecheskogo proiskhozhdeniia s virusom grippa