title: In vitro cytotoxicity studies in bladder and renal cell cancer.
abstract: Attempts to obtain cell lines from bladder and renal cancer patients were done in 42 patients, with success to obtain primary cultures in 25 and long-term cultures in 7. Cell-mediated and humoral immunity was demonstrated in both tumors by microcytotoxicity in vitro. No cytotoxicity could be deomonstrated against target cells obtained from metastatic cancer tissue. Abrogation of cell-mediated immunity by serum factors was demonstrated. No recurrence was obtained in the group of patients showing positive lymphocytotoxicity during follow-up.
mesh_terms: D000912:Antibodies, Neoplasm / Q000032:analysis; D000937:Antigen-Antibody Reactions; D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D007680:Kidney Neoplasms / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
chemical_list: D000912:Antibodies, Neoplasm; D003165:Complement System Proteins