DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment.
We have developed a method for permeabilizing CHO cells to nucleotides under conditions which allow most cells to remain viable. Permeabilized cells can carry out ATP-dependent, semiconservative synthesis of DNA. The data are consistent with the continuation of DNA synthesis in those cells in S phase at the time of treatment, possibly limited to completion of replicon synthesis without new initiations.
D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002470:Cell Survival; D004247:DNA / Q000096:biosynthesis*; D004260:DNA Repair; D003843:Deoxycytidine Monophosphate / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011136:Polysorbates / Q000494:pharmacology*
D003854:Deoxyribonucleotides; D011136:Polysorbates; D003843:Deoxycytidine Monophosphate; D011092:Polyethylene Glycols; D004247:DNA