title: Industrial experience with the detection of retroviruses.
abstract: An important aspect of the virological assessment of cell substrates used to produce biologicals is their retroviral status. In this presentation we will attempt to document some of the practical experiences of industry when testing cell substrates for retroviruses using infectivity tests, reverse transcriptase and electron microscopy. We will also explore some important aspects of the experimental design of these retrovirus assay systems and review some results from the application of these methods in model, experimental systems.
mesh_terms: D000818:Animals; D001709:Biotechnology; D016466:CHO Cells; D002460:Cell Line / Q000382:microbiology*; D006224:Cricetinae; D005069:Evaluation Studies as Topic; D006801:Humans; D008854:Microscopy, Electron; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012190:Retroviridae / Q000201:enzymology / Q000302:isolation & purification* / Q000472:pathogenicity; D012192:Retroviridae Infections / Q000209:etiology; D014773:Virology / Q000379:methods
chemical_list: D012194:RNA-Directed DNA Polymerase