Cytoplasmic expression of a CD24-related epitope in human PHA activated normal T lymphocytes.
In this study we have analysed by immunoperoxidase (IPx) and indirect immunofluorescence (IIF) the intracellular and cell surface reactivity of VIB-E3 mAb, previously clustered as anti-CD24 antigen, on resting and activated normal human T lymphocytes. By IPx assay VIB-E3 mAb did not show reactivity with normal resting T cells. In contrast, the analysis of 11 different samples of PHA activated normal mononuclear cells, showed an intracytoplasmic expression of CD24. Kinetic studies showed that CD24 appears 24 to 48 h after PHA stimulation. To our knowledge, this is the first evidence that a CD24-related epitope is expressed in normal activated T lymphocytes.
D015703:Antigens, CD / Q000096:biosynthesis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis; D018826:CD13 Antigens; D051927:CD24 Antigen; D017252:CD3 Complex / Q000096:biosynthesis; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation / Q000276:immunology*; D008562:Membrane Glycoproteins; D010835:Phytohemagglutinins / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D051927:CD24 Antigen; C497889:CD24 protein, human; D017252:CD3 Complex; D000939:Epitopes; D006684:HLA-DR Antigens; D008562:Membrane Glycoproteins; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2; D018826:CD13 Antigens