Reaction of HeLa cell methyl-labelled 28S ribosomal RNA with sodium bisulphite: a conformational probe for methylated sequences.
The reaction of 14C methyl-labelled HeLa cell 28 S ribosomal RNA with sodium bisulphite was studied. Using conditions under which 30% of the total cytidine residues were de-aminated to uridine, the reactivities of individual cytidine residues near particular methylation sites differed widely; some underwent almost quantitative reaction, some showed intermediate reactivity and others were almost inert. The possible value of this method as a conformational probe for ribosomal RNA is discussed.
D001483:Base Sequence; D001665:Binding Sites; D006367:HeLa Cells; D008745:Methylation; D009843:Oligoribonucleotides / Q000032:analysis; D012335:RNA, Ribosomal; D013447:Sulfites
D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D013447:Sulfites