[Isolation of phospholipase D from cotton seeds].
A method of isolating homogenous phospholipase D from cotton seeds is described. The homogeneity of the enzyme preparation is demonstrated by means of polyacrylamide disc electrophoresis, gel filtration through Sephadex G-200 and determination of N-terminal amino acid residue, which turned to be glutamic acid. Molecular weight of the enzyme is found to be 71000 +/- 3000. Several equilibrium forms of the enzyme are observed.
D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D003368:Gossypium / Q000201:enzymology*; D010740:Phospholipases / Q000302:isolation & purification*; D012639:Seeds / Q000201:enzymology*
D010740:Phospholipases
Vydelenie fosfolipazy D iz semian khlopchatnika