title: Characterization of midbrain component of the trigger for arousal from hibernation.
abstract: The ability of the midbrain reticular formation (MRF) to trigger arousal from hibernation and to change body temperature (Tb) during euthermia was tested in golden-mantled ground squirrels (Citellus lateralis). During hibernation (ambient temperature 5-6 degrees C) microinjections of cholinomimetic compounds (acetylcholine and carbachol) triggered full arousal or produced transient increases in Tb in 15 out of 19 tests; MRF microinjections of norepinephrine (NE) and 5-hydroxytryptamine (5-HT), at the same concentrations that produced full arousal when microinjected into the preoptic/anterior hypothalamus (PO/AH) area in previous experiments, has no effect on Tb in 13 out of 16 tests. In experiments on euthermic ground squirrels tested at an ambient temperature of 25 degrees C, MRF microinjections of acetylcholine raised Tb, whereas the monoamines had no significant effects. These results show that cholinoceptive neurons in the MRF can trigger arousal from hibernation and increase Tb during euthermia. The failure of NE and 5-HT to produce any significant effects at the same doses that were reported to be effective in the PO/AH indicates that the functional organization of the MRF portion of the arousal mechanism is different from that reported for the PO/AH.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001143:Arousal / Q000502:physiology*; D001831:Body Temperature / Q000187:drug effects; D002217:Carbachol / Q000494:pharmacology; D003404:Creatinine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D008636:Mesencephalon / Q000033:anatomy & histology / Q000502:physiology*; D008845:Microinjections; D009638:Norepinephrine / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013268:Stimulation, Chemical; D013644:Tartrates / Q000494:pharmacology
chemical_list: D013644:Tartrates; D012701:Serotonin; D012965:Sodium Chloride; D002217:Carbachol; D003404:Creatinine; D000109:Acetylcholine; D009638:Norepinephrine