Effect of long-wave ultraviolet light on the lens. I. Model systems for detecting and measuring effect on the lens in vitro.
Rat mouse, and chick lenses incubated with 3-aminotriazole under long-wave ultraviolet (UV) show reduced accumulation and incorporation of leucine and a loss of glutathione. The effect on leucine incorporation is strikingly enhanced when capsule-epithelium pools are incubated. The procedure may identify photosensitizers or metabolic inhibitors which are cataractogenic when acting in conjunction with UV.
D000640:Amitrole / Q000494:pharmacology; D000818:Animals; D002374:Catalase / Q000037:antagonists & inhibitors; D002642:Chick Embryo; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000378:metabolism / Q000528:radiation effects*; D007930:Leucine / Q000378:metabolism; D051379:Mice; D011830:Radiation Effects; D051381:Rats; D014466:Ultraviolet Rays
D002374:Catalase; D005978:Glutathione; D007930:Leucine; D000640:Amitrole