Effect of cytochrome C oxidase and polyanions on the alkaline transition of ferricytochrome C.
Application of heparin, polyadenylate, polyglutamate and polygalacturonate resulted in changes in the electron absorption spectrum of cytochrome c that resembled those after cytochrome c oxidase application at neutral pH. The formed complexes of cytochrome c with polyanions retain the bond of Met-80 with heme iron. Cytochrome c oxidase and the polyanions increased the apparent pKa of alkaline transition of cytochrome c by an order of magnitude.
D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000737:chemistry* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000494:pharmacology*; D006493:Heparin / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009206:Myocardium / Q000201:enzymology; D009994:Osmolar Concentration; D010368:Pectins / Q000378:metabolism / Q000494:pharmacology; D011061:Poly A / Q000378:metabolism / Q000494:pharmacology; D000071228:Polyelectrolytes; D011099:Polyglutamic Acid / Q000378:metabolism / Q000494:pharmacology; D011108:Polymers / Q000378:metabolism / Q000494:pharmacology*; D013053:Spectrophotometry
D003574:Cytochrome c Group; D003576:Electron Transport Complex IV; D006493:Heparin; D010368:Pectins; D011061:Poly A; D000071228:Polyelectrolytes; D011099:Polyglutamic Acid; D011108:Polymers; C003181:polygalacturonic acid