Assessment of the genotoxic potential of riboflavin and lumiflavin. A. Effect of metabolic enzymes.
The mutagenic potential of riboflavin and its photodegradation product lumiflavin was evaluated using the umu test, SOS chromotest and Ames Salmonella assay. Both riboflavin and lumiflavin by themselves were found to be non-mutagenic. On treatment with rat liver microsomal enzymes (S9) or caecal cell-free extract (CCE), lumiflavin acquired mutagenicity, while the status of riboflavin remained unaffected. Activation of lumiflavin by metabolic enzymes was found to result in an alteration of its spectral characteristics.
D000469:Alkaline Phosphatase / Q000502:physiology; D000818:Animals; D002432:Cecum / Q000201:enzymology*; D002855:Chromatography, Thin Layer; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005415:Flavins / Q000633:toxicity*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009152:Mutagenicity Tests; D051381:Rats; D017208:Rats, Wistar; D012256:Riboflavin / Q000633:toxicity*; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium; D001616:beta-Galactosidase / Q000502:physiology
D005415:Flavins; C001922:lumiflavin; D004247:DNA; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase; D012256:Riboflavin