ENU mutagenesis in the mouse electrophoretic specific-locus test. 2. Mutational studies of mature oocytes.
Experiments were conducted using the biochemical specific-locus test to assess the mutagenicity of N-ethyl-N-nitrosourea (ENU) in mature oocytes of mice. C57Bl/6J females were treated with 100 mg/kg ENU by intraperitoneal injection and mated to untreated DBA males for 1 week following treatment. 1447 progeny were screened for evidence of mutations affecting the electrophoretic mobility of 32 different proteins; two mutants were detected by electrophoretic analyses. These results provide evidence that ENU is a germ-cell mutagen in mouse mature oocytes, although the frequency of mutants is somewhat lower than that obtained from spermatogonia treated with the same dose.
D000818:Animals; D003433:Crosses, Genetic; D004586:Electrophoresis; D005038:Ethylnitrosourea / Q000633:toxicity*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D010641:Phenotype
D005038:Ethylnitrosourea