Immobilized glucose oxidase used in the continuous-flow determination of serum glucose.
We used a tubular glucose-oxidase wall reactor in the "AutoAnalyzer II" continuous-flow analytical system to determine glucose in blood serum. Sensitivity was high and wash characteristics were satisfactory with use of a 30-cm tube containing immobilized glucose oxidase. Results compared favorably with those of the conventional free-enzyme method. More than 25000 such assays can be performed with a single enzyme tube, which also shows long-term storage stability. Because of the steady-state chemistry 60 samples can be analyzed per hour. The linearity of the method is excellent and sample interaction from 5.0 to 1.0 g/liter is less than 5%. Results correlate well (greater than 0.993) with those obtained with both the neocuproine method used in the "SMA 12/60" multichannel analytical system and the free glucose oxidase method used in the AutoAnalyzer.
D001322:Autoanalysis; D001786:Blood Glucose / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D005949:Glucose Oxidase / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D012044:Regression Analysis; D012995:Solubility
D001786:Blood Glucose; D005949:Glucose Oxidase