[Carbonic anhydrase--marker of cell type in cell culture of the guinea pig vas deferens].
Polyclonal antibodies (PCAB) to smooth muscle myosin (SMM), monoclonal antibodies (MCAB) to cytokeratin 8 (clon HI, IgGI) and H4 (IgM), as well as PCAB to carbonic anhydrase III were used for identification of the cell types in the vas deferens cell culture of guinea pig. Smooth muscle cells (SMC) are identified by intensive staining of PCAB to SMM. Fibroblast-like cells (FBL) are determined by the presence of the filament finest network, apparently responding to the myosin non-muscular forms, which are present in PCAB to SMM. The epithelial cells are stained by MCAB to cytokeratins. PCAB to carbonic anhydrase III interact with all three cell types. In the majority of SMC the enzyme is detected as solitary stripes, though there are diffuse ones across the whole cytoplasms, the nucleus remains clearly visible. Carbonic anhydrase III in epithelial cells is detected only in nucleoli and along nucleus membrane while in FBL--in nucleoli and cytoplasm as focal granulation. PCAB to carbonic anhydrase III may serve as a universal marker for identification of cell type in the guinea pig vas deferens cell culture.
D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis*; D002478:Cells, Cultured; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D009130:Muscle, Smooth / Q000166:cytology; D013194:Staining and Labeling; D014649:Vas Deferens / Q000166:cytology*
D002256:Carbonic Anhydrases
Karboangidraza--marker kletochnykh tipov v kul'ture kletok semiavynosiashchego protoka morskikh svinok.