Separation of C19 and C21 dihydroxysteroids by open-hole tubular glass columns and lipophilic gel chromatography.
The combination of lipophilic gel chromatography and high-resolution glass capillary chromatography has been shown to be an important means of identification and quantification of steroid urinary metabolites. Some problems in the use of capillary columns, such as coating with polar and apolar stationary phases, column performance, thermal stability and injection systems have been investigated and this type of chromatography has been used in the analysis of 33 dihydroxysteroids. Gel chromatography is a method complementary to high-efficiency gas chromatography. The steroids are eluted according to the number of hydroxyl groups in the molecules.
D015113:Androstane-3,17-diol / Q000652:urine; D000731:Androstanes / Q000652:urine; D002850:Chromatography, Gel / Q000295:instrumentation*; D005898:Glass; D006914:Hydroxysteroids / Q000652:urine*; D008433:Mathematics; D011276:Pregnanediol / Q000652:urine; D011278:Pregnanes / Q000652:urine; D012997:Solvents
D000731:Androstanes; D006914:Hydroxysteroids; D011278:Pregnanes; D012997:Solvents; D015113:Androstane-3,17-diol; D011276:Pregnanediol