Binding of morphine by serum globulins from morphine-treated rabbits. I. Induction and measurement of binding.
Treatment of rabbits with morphine under conditions defined in this report leads to significant and reproducible increases in the binding of morphine by serum globulins. Complexes formed are readily dissociable so that the conditions employed in measuring binding become critical. The data presented document the effects of several variables upon the results of binding measurements. Also described are treatments that lead either to "priming" or to the inhibition of a subsequent response.
D000818:Animals; D001665:Binding Sites; D003956:Dialysis; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D006241:Haptens / Q000032:analysis; D007279:Injections, Subcutaneous; D008297:Male; D009020:Morphine / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D004856:Postural Balance; D011485:Protein Binding; D011817:Rabbits; D011863:Radioimmunoassay; D012709:Serum Albumin / Q000494:pharmacology; D012712:Serum Globulins / Q000494:pharmacology*
D006241:Haptens; D012709:Serum Albumin; D012712:Serum Globulins; D009020:Morphine