An improved HRP method for tracing axons in whole-mount preparations during early stages of regeneration in peripheral nerves of adult animals.
An improved method using HRP for visualizing axons in whole-mount preparations is described. Application of this method to preparations of regenerating frog peripheral nerves maintained in organ culture was used to show that regeneration following nerve crush begins after about 3 days.
D000818:Animals; D001369:Axons / Q000648:ultrastructure*; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D008867:Microtomy; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000502:physiology*; D010525:Peripheral Nerves / Q000166:cytology* / Q000502:physiology; D011896:Rana temporaria; D013194:Staining and Labeling
D006735:Horseradish Peroxidase