Induction of long-term survival of rat skin allografts by a novel, highly efficient anti-CD4 monoclonal antibody.
The new monoclonal antirat CD4 antibody RIB 5/2, which detects another epitope than those covered by W3/25 and MRC OX35, was tested for its immunosuppressive potency following skin allografting by using strain combinations with different genetic barriers in the MHC and genetic low- or high-responder background. High-dose and long-term therapy of the grafted rats led to a significant delay of the acute rejection (P < 0.01) in the strain combination Wistar Furth-to-BDX as well as in LEW1W-to-LEW1A. No significant prolongation of the mean allograft survival time was obtained for the high-responder rats (LEW1A-to-LEW1W). Cytofluorometric analysis revealed that RIB 5/2 exerts the immunosuppressive activity predominantly by modulation of the CD4 glycoprotein. Furthermore, the dependence of the humoral immune response against the mouse-globulins upon the administered protein quantity could be demonstrated.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000917:Antibody Formation / Q000276:immunology; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006085:Graft Survival; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D010641:Phenotype; D051381:Rats; D011917:Rats, Inbred Lew; D011920:Rats, Inbred WF; D016038:Skin Transplantation / Q000276:immunology*; D013997:Time Factors; D014184:Transplantation, Homologous / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D000939:Epitopes; D007130:Immunoglobulin Idiotypes