Methisoprinol-effect on the replication cycle of human hepatitis A virus.
The antiviral activity of methisoprinol was investigated under different conditions using a strain of hepatitis A virus (HAV), that shows a strong cytopathic effect on the Frp/3 cell line 7-9 days post-infection. Treatment of Frp/3 at a dose range of 125-1200 micrograms/ml had no toxic effect and showed a dose dependent inhibition of the HAV replication cycle. At the methisoprinol dose of 500 micrograms/ml the cytopathic effect was completely abolished and HAV antigen production reduced by 50% as measured by indirect immunofluorescence (IIF) and commercial enzyme-linked assay (ELISA). The virus yield was virtually abolished at the highest dose employed (1000 micrograms/ml).
D000818:Animals; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D006507:Hepatovirus / Q000187:drug effects*; D007542:Inosine Pranobex / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D007542:Inosine Pranobex