Engineering of artificial cell adhesion proteins by grafting the Arg-Gly-Asp cell adhesive signal to a calpastatin segment.
A new artificial cell adhesive protein was engineered by grafting the Arg-Gly-Asp (RGD) sequence, the minimal recognition signal of fibronectin for interaction with integrins, to a calpastatin segment by in vitro mutagenesis. The mutagenized protein showed cell adhesive activity in addition to calpain inhibitory activity. The RGD signal grafted to the calpastatin segment was recognized by the vitronectin receptor but not by the fibronectin receptor.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000502:physiology*; D002154:Calpain / Q000037:antagonists & inhibitors; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D009842:Oligopeptides / Q000235:genetics / Q000502:physiology*; D016024:Receptors, Cytoadhesin / Q000502:physiology; D017469:Receptors, Fibronectin / Q000502:physiology; D019030:Receptors, Vitronectin; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000494:pharmacology / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology
D002135:Calcium-Binding Proteins; D015815:Cell Adhesion Molecules; D009842:Oligopeptides; D016024:Receptors, Cytoadhesin; D017469:Receptors, Fibronectin; D019030:Receptors, Vitronectin; D011993:Recombinant Fusion Proteins; C047981:arginyl-glycyl-aspartic acid; C033668:calpastatin; D002154:Calpain