Photoaffinity labeling of the beta-adrenergic receptors and receptor reserve for isoprenaline.
The beta-adrenergic receptors in the taenia isolated from the guinea pig caecum were irreversibly inactivated by photoaffinity labeling with 1-isoprenaline and 2-(2-hydroxy-3-isopropylaminopropoxy)iodobenzene. The photoinactivation was eliminated in the presence of protectors of the receptors. Photoinactivation caused a considerable parallel shift in the concentration-action curve of 1-isoprenaline. The results possibly point to a certain receptor reserve for 1-isoprenaline.
D000345:Affinity Labels / Q000494:pharmacology*; D000818:Animals; D002432:Cecum / Q000187:drug effects; D003106:Colon / Q000187:drug effects; D066298:In Vitro Techniques; D007462:Iodobenzenes / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D010782:Photolysis; D011412:Propanolamines / Q000494:pharmacology*; D011941:Receptors, Adrenergic / Q000187:drug effects*; D011955:Receptors, Drug / Q000187:drug effects
D000345:Affinity Labels; D007462:Iodobenzenes; D011412:Propanolamines; D011941:Receptors, Adrenergic; D011955:Receptors, Drug; C011861:2-(2-hydroxy-3-isopropylaminopropoxy)iodobenzene; D007545:Isoproterenol