Development of a competitive radioimmunoassay and a sandwich enzyme-linked immunosorbent assay for recombinant human granulocyte colony-stimulating factor. Application to a pharmacokinetic study in rats.
A competitive radioimmunoassay (RIA) and a sandwich enzyme-linked immunosorbent assay (ELISA) for the measurement of recombinant human granulocyte colony-stimulating factor (rhG-CSF) in rat serum was developed using polyclonal antibody obtained by immunizing rabbits with rhG-CSF. The anti-rhG-CSF antibody did not recognize other colony-stimulating factors or interleukins. RIA and ELISA gave satisfactory reproducibility as judged by intra- and inter-assay precision. Good agreements between the RIA, ELISA and bioassay were observed with rat serum samples after administration of rhG-CSF. The competitive RIA and sandwich ELISA described here appear to be as equally useful as the bioassay in studying the pharmacokinetics of rhG-CSF in animals.
D000818:Animals; D001681:Biological Assay; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood*; D008297:Male; D051379:Mice; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000097:blood
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor