Use of monoclonal antibodies for discrimination between natural and recombinant human interferon-tau.
Different monoclonal antibodies (MAbs) were raised against denaturated and native recombinant human Interferon-tau (IFN-tau). This approach gave us MAbs which recognized either N-term (prepared with SDS-denaturated IFN-tau) or C-terminal part of the antigen as well as MAbs which linked to non linear epitopes (obtained with native form of IFN-tau). Some of them inhibited or enhanced their respective binding to IFN-tau. After characterization, these antibodies were used as probes and some were selected to prepare two quantitative sensitive sandwich IRMAs able to discriminate between recombinant and natural IFN-tau.
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D015592:Immunoradiometric Assay / Q000379:methods; D007371:Interferon-gamma / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D015335:Molecular Probes; D009500:Neutralization Tests; D011489:Protein Denaturation; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015335:Molecular Probes; D011994:Recombinant Proteins; D007371:Interferon-gamma