Characterization and replication control of plasmids from Enterobacter cloacae DF 13.
It has been shown previously that Enterobacter clocacae DF13 harbours at least five different size classes of plasmids. A 45 x 10(6)-Mr self-transmissible R factor determining resistance against tetracyclin, sulfanilamide, streptomycin and chloramphenicol, a 6.0 x 10(6)-Mr bacteriocinogenic factor without sex factors activity and cryptic plasmids in the size classes of Mr 1.3 x10(6), 2.8 x 10(6) and 8.0 x 10(6) respectively. The present work deals with the determination of the homogeneity and molecular relationship of 1.3 x 10(6)-Mr (mini) and 2.8 x 10(6)-Mr (midi) cryptic plasmids and the 6.0 x 10(6)-Mr (maxi) bacteriocinogenic factor, their kinetics of replication and their replication control in response to inhibition of protein synthesis.
D001483:Base Sequence; D002701:Chloramphenicol / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000378:metabolism; D004261:DNA Replication; D004755:Enterobacteriaceae / Q000378:metabolism*; D005111:Extrachromosomal Inheritance; D007700:Kinetics; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D010957:Plasmids; D011815:R Factors / Q000187:drug effects; D013307:Streptomycin / Q000494:pharmacology; D013424:Sulfanilamides / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology
D013424:Sulfanilamides; D002701:Chloramphenicol; D013752:Tetracycline; D013307:Streptomycin