Interferon-neutralizing or enhancing activities in hybridoma cell fluids after in vitro immunization.
Several hybridomas supernatants capable of interferon beta (IFN-beta) or "IFN epsilon" ("IFN-eps") neutralizing or enhancing activities were obtained after in vitro immunization of BALB/c and C57 mice spleen cells and their fusion with Sp2/0 plasmacytoma cells. Besides rather low anti-IFN-beta or "eps" antibody secretion several cloned hybridoma fluids contained a factor potentiating anti-viral activity of the both IFNs. It is speculated that this activity is due to production by some hybridomas of another lymphokine.
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D016899:Interferon-beta / Q000037:antagonists & inhibitors / Q000276:immunology*; D007372:Interferons / Q000037:antagonists & inhibitors / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D016899:Interferon-beta; D007372:Interferons