A new technique for dissociation of hair follicles into single cells.
A method is described for the complete dissociation of mouse hair follicles into a suspension of single cells suitable for cell culture. Dermal tissue containing hair follicles is digested sequentially with trypsin and chondroitinase ABC under mild conditions.
D000818:Animals; D002469:Cell Separation; D002808:Chondroitin Lyases; D046508:Culture Techniques / Q000379:methods*; D006197:Hair; D051379:Mice; D012867:Skin; D014357:Trypsin
D014357:Trypsin; D002808:Chondroitin Lyases