Correlation of in vivo malignancy with in vitro properties of human-mouse hybrid cells.
The in vivo tumorigenicity of malignant mouse-nonmalignant human somatic cell hybrids was correlated with the in vitro characteristics. The renal adenocarcinoma mouse cell line RAG and the normal, diploid human cell line Wl-38 were used as the fusion parents. Five independent RAG Wl 38 hybrid clones were tested for concanavalin A (Con A) agglutination patterns, in vitro invasiveness, and tumor formation in immunosuppressed mice. Tests on the parental lines showed that RAG cells agglutinated at much lower levels of Con A than the Wl-38 cells. RAG cells overgrew Wl-38 cells in the in vitro invasiveness assay. RAG cells readily formed tumors in untreated adult or young immunosuppressed mice, whereas the Wl-38 cells did not. The five hybrid clones were all similar, since they had Con A agglutination levels intermediate to those of both parents, though patterns were more "tumor-like", overgrew the Wl-38 cells in the invasiveness assay, and formed tumors in immunosuppressed mice.
D000230:Adenocarcinoma / Q000209:etiology; D000371:Agglutination; D000818:Animals; D002455:Cell Division; D002875:Chromosomes / Q000648:ultrastructure; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D007165:Immunosuppression Therapy; D007680:Kidney Neoplasms / Q000209:etiology; D008297:Male; D051379:Mice; D009374:Neoplasms, Experimental / Q000209:etiology*
D003208:Concanavalin A