Interleukin-2 inhibits the GABA-induced Cl- current in identified Aplysia neurons.
The effects of extracellularly applied recombinant human interleukin-2 (rhIL-2) on the gamma-aminobutyric acid (GABA)-induced Cl- current recorded from identified neurons (R9 and R12) of Aplysia kurodai were investigated with conventional voltage-clamp and pressure ejection techniques. Bath-applied rhIL-2 (10-40 U/ml) reduced the GABA-induced current in the neurons without affecting resting membrane conductance and the holding current. The suppressing effect of rhIL-2 on the current was completely reversible. Heat-inactivated rhIL-2 was without effect. These results suggest that the immunomodulator IL-2 can modulate the GABA-induced response in the nervous system.
D000818:Animals; D001048:Aplysia / Q000378:metabolism*; D001640:Bicuculline / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism*; D018756:GABA Antagonists; D005724:Ganglia / Q000166:cytology / Q000187:drug effects; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects*; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D009118:Muscimol / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism*; D010852:Picrotoxin / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D012623:Seawater; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D001640:Bicuculline; D002712:Chlorides; D018756:GABA Antagonists; D007376:Interleukin-2; D007473:Ion Channels; D009118:Muscimol; D010852:Picrotoxin; D011994:Recombinant Proteins; D005680:gamma-Aminobutyric Acid