[The fractional composition of immunoglobulin preparations studied by gel filtration on different carriers and by high-pressure liquid chromatography].
The fractional composition of immunoglobulin preparations produced by different manufacturing enterprises of this country has been studied by gel chromatography in columns packed with different carriers (Sephadex G-200 and ultragel AcA-34) and by high-performance liquid chromatography (HPLC). This study has revealed the nonstandard character of immunoglobulin preparations produced according to the same technological procedure (modified Cohn's method). The fractionation of immunoglobulins on different carriers with the use of different methods has yielded similar results confirmed by the statistical processing of the data. The results obtained in the study of the fractional composition of immunoglobulin preparations evidence that gel filtration with the use of ultragel and HPLC have greater resolving capacity in comparison with the method of gel filtration on traditionally used Sephadex G-200.
D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003911:Dextrans; D005069:Evaluation Studies as Topic; D005782:Gels; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D007202:Indicators and Reagents; D009930:Organic Chemicals; D011786:Quality Control
D003911:Dextrans; D005782:Gels; D007136:Immunoglobulins; D007202:Indicators and Reagents; D009930:Organic Chemicals; C079139:Ultragel AcA-34; C025614:sephadex
Izuchenie fraktsionnogo sostava preparatov immunoglobulinov metodom gel'-fil'tratsii na razlichnykh nositeliakh i metodom zhidkostnoĭ khromatografii vysokogo davleniia.