cAMP-mediated second messenger mechanisms are involved in spinule formation in teleost cone horizontal cells.
A number of light adaptive changes of teleost horizontal cells are mediated by dopamine D1 receptors coupled positively with the cAMP second messenger system. Spinules, finger-like extensions from horizontal cell dendrites directed towards the cone pedicle cytoplasm, are formed in response to a stimulation of D1 receptors. We studied the second messenger mechanism associated with this process using isolated dark-adapted cyprinid retinae. Increasing intracellular cAMP concentrations by adding a membrane permeable analogue, or by stimulating the adenylate cyclase and simultaneously blocking the degradation of cAMP, resulted in a significant increase of spinule numbers in spite of the absence of light. In contradistinction to using isolated retinae for pharmacological experiments, injection of drugs into the vitreous had inconsistent or negative results.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000221:Adaptation, Ocular / Q000502:physiology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D004298:Dopamine / Q000502:physiology; D005399:Fishes / Q000502:physiology*; D008854:Microscopy, Electron; D009434:Neural Pathways / Q000166:cytology / Q000502:physiology; D010786:Photoreceptor Cells / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D017447:Receptors, Dopamine D1 / Q000187:drug effects; D015290:Second Messenger Systems / Q000502:physiology*
D017447:Receptors, Dopamine D1; D005576:Colforsin; D003994:Bucladesine; D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine; D004298:Dopamine