[Electron microscope studies of the kidney after its preservation at low temperatures].
Fragments the renal cortical part of white rats were frozened in two solutions: saline and 15% of glycerol. The preservation was made in liquid nitrogen (--196 degrees) for a period of 24 hours. The obtained results from the electrone microscopic examination showed that when freezing was performed without protective media, the intactness of the cells and their ultrastructural organization were destroyed. The nuclei preserved their intactness. When protective media (15% of glycerol) was used, then greater stability to low temperatures revealed the epitheleal cells, forming the glomerulus and intersticial tubules, but the most sensitive were the epithelial cells in the main part of the nephrone.
D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D005615:Freezing; D007668:Kidney / Q000648:ultrastructure*; D007678:Kidney Glomerulus / Q000648:ultrastructure; D008854:Microscopy, Electron; D009399:Nephrons / Q000648:ultrastructure; D009926:Organ Preservation; D051381:Rats; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation
Elektronnomikroskopski izsledvaniia vurkhu bubrek sled konserviraneto mu pri niski temperaturi