Dissociation-constants of metat-ion-complexes with alkaline phosphatase from pig kidney.
Using metal-ion buffers it was possible to remove Zn2+, Mg2+ and Mn2+ ions of pig kidney alkaline phosphatase reversibly. The dissociation constants obtained are KEMg: 4 X 10(-7) M, KEMn: 4 X 10(-8) M and KEZn: 8 X 10(-13) M (22 degrees C, pH: 9.6, mu: 0.07).
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent; D007501:Iron / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D013552:Swine; D015032:Zinc / Q000494:pharmacology
D002413:Cations, Divalent; D008345:Manganese; D007501:Iron; D000469:Alkaline Phosphatase; D008274:Magnesium; D015032:Zinc; D002118:Calcium