title: False-positive rabbit ileal loop reactions attributed to Vibrio parahaemolyticus broth filtrates.
abstract: Vibrio parahaemolyticus broth filtrates have previously been shown to produce positive reactions in rabbit ileal loops only if concentrated 10-fold by lyophilization. This method of concentration produces solutions that contain greater than 20% NaCl. In the present study, however, concentrations of NaCl of greater than or equal to 4% induced positive responses in ileal loops, and desalting rendered previously reactive, concentrated broth filtrates negative. Therefore, enterotoxin was not demonstrated in our broth filtrates of V. parahaemolyticus, a finding which suggests that previous studies require further evaluation. Since most culture media contain 0.5% NaCl, it is important to determine and to control the NaCl content and the osmolality of all lyophilized concentrates tested in the ligated rabbit ileum.
mesh_terms: D000818:Animals; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000494:pharmacology; D005189:False Positive Reactions; D005612:Freeze Drying; D007082:Ileum / Q000187:drug effects / Q000382:microbiology*; D008297:Male; D008954:Models, Biological; D011817:Rabbits; D012462:Saline Solution, Hypertonic; D012965:Sodium Chloride / Q000494:pharmacology; D014735:Vibrio Infections / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000472:pathogenicity*; D014774:Virulence; D014882:Water-Electrolyte Balance / Q000187:drug effects
chemical_list: D003470:Culture Media; D004768:Enterotoxins; D012462:Saline Solution, Hypertonic; D012965:Sodium Chloride