Purification and characterization of a sperm-coating antigen from human seminal plasma.
A sperm-coating antigen has been purified from human seminal plasma by a combination of fractionation methods including ammonium sulfate precipitation, Sephadex G-100 gel filtration, and diethylaminoethyl cellulose ion-exchange chromatography. The purified antigen was demonstrated to be immunologically identical sperm-coating antigen, not derived from blood serum but having immunologically cross-relating antigens in milk and saliva, and a glycoprotein with a molecular weight of approximately 33,000. The purpose of purifying this antigen was discussed.
D000941:Antigens / Q000032:analysis / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007122:Immunoelectrophoresis; D008297:Male; D008970:Molecular Weight; D012661:Semen / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*
D000941:Antigens; D006023:Glycoproteins