The effect of steroids on adhesiveness, rosette-forming ability and survival of cultured, human mononuclear cells.
The effect of different steroids on human mononuclear blood cells during the first 90 minutes of culture in vitro was tested. Cortisol and testosterone were found to increase the ability of lymophocytes to adhere on plastic surfaces. None of the other steroids tested exhibited this effect. Cortisol and corticosterone were found to reduce the number of surviving macrophages in the culture dishes, tested 4 to 8 days after the exposure to the drugs. No lysis of mononuclear cells could be detected following addition of cortisol in doses up to 10(-1) mg/ml. The findings support the previous statements that human lymphocytes are more cortisol-resistant than those of mouse, rat and rabbit.
D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003345:Corticosterone / Q000494:pharmacology; D003900:Desoxycorticosterone / Q000494:pharmacology; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007104:Immune Adherence Reaction; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D013256:Steroids / Q000494:pharmacology*; D013739:Testosterone / Q000494:pharmacology; D013760:Tetrahydrocortisol / Q000494:pharmacology
D013256:Steroids; D013739:Testosterone; D003900:Desoxycorticosterone; D013760:Tetrahydrocortisol; D003345:Corticosterone; D006854:Hydrocortisone