[The fractionation of non-histone chromosomal proteins from rat kidney and liver and their DNA complexes by using the cation exchangers phosphocellulose and SE-Sephadex].
Results of the gradient chromatography of chromosomal nonhistone proteins of the rat liver and kidney and their complexes with DNA on the phosphocellulose and SE-Sephadex are presented. The profiles of elution of the preparation of the homologous tissues with NaCl linear gradient of 0.1-1.0 M are equal. In the fractions of 0.4-0.5 M NaCl, protein components are discovered only in the samples of kidney.
D000818:Animals; D002411:Cation Exchange Resins; D002458:Cell Fractionation / Q000379:methods; D002482:Cellulose / Q000031:analogs & derivatives; D002852:Chromatography, Ion Exchange / Q000379:methods; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D004247:DNA / Q000302:isolation & purification*; D003911:Dextrans; D005782:Gels; D007202:Indicators and Reagents; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D008297:Male; D008970:Molecular Weight; D051381:Rats
D002411:Cation Exchange Resins; D002868:Chromosomal Proteins, Non-Histone; D003911:Dextrans; D005782:Gels; D007202:Indicators and Reagents; D002482:Cellulose; D004247:DNA; C025614:sephadex; C008881:phosphocellulose
Fraktsionirovanie khromosomnykh negistonovykh belkov pochek i pecheni krys i ikh kompleksov s DNK s ispol'zovaniem kationitov fosfotselliulozy i SE-sefadeksa.