A 25 kDa alpha 2-microglobulin-related protein is a component of the 125 kDa form of human gelatinase.
Besides the monomeric mammalian 95 kDa progelatinase, two additional forms, a disulfide-bridged 220 kDa dimer and a 125 kDa form were isolated from human PMN leukocytes. The 125 kDa progelatinase was identified as a covalently linked, disulfide-bridged heterodimer formed of the monomer with a 25 kDa protein. This 25 kDa protein was isolated from gelatinase bound to the affinity support of gelatin-Sepharose and eluted by DTE-containing buffer. The amino acid sequence of tryptic peptides of this protein revealed homology with an alpha 2-microglobulin-related protein from rats, a protein so far unknown in humans.
D000510:Alpha-Globulins / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D017364:Collagenases / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid
D000510:Alpha-Globulins; C025500:alpha(2)-microglobulin; D017364:Collagenases; D020780:Matrix Metalloproteinase 9