Radioimmunoassays for free and conjugated trienbolone and for trienbolone acetate in bovine tissue and plasma samples.
A specific, sensitive, precise and accurate radioimmunoassay has been developed for the quantitation of the synthetic anabolic steroid trienbolone acetate (TBA) and its major metabolites, free and conjugated trienbolone (TBOH) in bovine tissues and plasma. With the extraction procedure described unspecific interference with the antigen-antibody reaction could be ruled out. The assay can significantly detect amounts of more than 40 pg TBOH and 70 pg TBA. 0.1 - 2.0 g tissue and 0.1 - 1 ml plasma are sufficient for 1 determination. Residues (range 0.1 -2.0 ng/g) were still present in calves 69 days after implantation of "Revalor" (20 mg estradiol-17beta and 140 mg TBA) with the highest concentrations found in liver and TBA could only be quantitated in fat.
D000273:Adipose Tissue / Q000378:metabolism; D045930:Anabolic Agents / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D004958:Estradiol / Q000378:metabolism; D004963:Estrenes / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D004967:Estrogens; D007106:Immune Sera; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008832:Microchemistry; D009132:Muscles / Q000378:metabolism*; D011863:Radioimmunoassay
D045930:Anabolic Agents; D004963:Estrenes; D004967:Estrogens; D007106:Immune Sera; D004958:Estradiol