title: Demonstration of macrophage chemotactic factors in the aqueous humor during experimental immunogenic uveitis in rabbits.
abstract: Factors in the queous humor capable of increasing the random migration and chemotaxis of monocytes were demonstrated during experimental uveitis in the rabbit. Injection of endotoxin-free human serum albumin into the vitreous produced a severe inflammation detectable after 7 days and maximal between 12 to 21 days. Aqueous humor was measured for chemotactic activity with a modified Boyden chamber, which permitted the use of volumes of less than 0.025 ml. Chematactic activity was measurable during the primary inflammation and remained elevated for a short period of time after clinical inflammation and anterior chamber cellular reaction had decreased. A severe reinduction uveitis could be initiated by the intravenous injection of human serum albumin after day 30. Chemotactic factors could be measured at high titers by 8 h after this intravenous challenge.
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000276:immunology*; D002465:Cell Movement; D002633:Chemotaxis; D008264:Macrophages / Q000276:immunology*; D008297:Male; D011817:Rabbits; D012709:Serum Albumin; D014605:Uveitis / Q000209:etiology / Q000276:immunology*
chemical_list: D012709:Serum Albumin