title: The reliability of a mechanized procedure (Perkin-Elmer C4) for the Enzymatic determination of uric acid according to Kageyama.
abstract: The enzymatic determination of the uric acie concentration in urine and serum according to Kageyama ((1971), Clin. Chim. Acta 31, 421--426), which excludes the deproteinization of samples, was adapted to the C4 automatic analyzer (Perkin-Elmer). The reliability of this procedure and its correlation with an UV-method were investigated. The interaction from-sample-to-sample was considerable, but this could be reduced by the addition of Brij-35. The recovery of uric acid added to protein-containing samples (about 96%) was better than with the UV-method. Novaminsulfone was the only substance tested which interfered significantly.
mesh_terms: D001322:Autoanalysis / Q000379:methods; D001798:Blood Proteins; D002374:Catalase; D003951:Diagnostic Errors; D004177:Dipyrone / Q000097:blood; D004355:Drug Stability; D006801:Humans; D007202:Indicators and Reagents; D014021:Tissue Preservation; D014503:Urate Oxidase; D014527:Uric Acid / Q000032:analysis*
chemical_list: D001798:Blood Proteins; D007202:Indicators and Reagents; D014527:Uric Acid; D004177:Dipyrone; D002374:Catalase; D014503:Urate Oxidase