Preparative isotachophoretic separation of skin test antigens from blastomycin purified derivative.
This investigation examined the theoretical and practical parameters requisite to preparatory istachophoretic separation of blastomycin purified derivative. It resulted in a relatively simple, two-step procedure for preparation of blastomycin antigens in milligram quantitites that exhibited sensitivity and specificity in experimentally infected guinea pigs. Analysis of the nine isotachophoretic fractions for skin test sensitivity and specificity provided some insight into the generally accepted unreliability of blastomycin when used for immunological evaluation.
D000818:Animals; D000946:Antigens, Fungal / Q000302:isolation & purification*; D001758:Blastomyces; D004591:Electrophoresis, Polyacrylamide Gel; D005657:Fungal Vaccines / Q000032:analysis; D006168:Guinea Pigs; D007525:Isoelectric Focusing; D012882:Skin Tests
D000946:Antigens, Fungal; D005657:Fungal Vaccines