16-sulfates of estriol in body fluids of human pregnancy at term.
A method was developed for the assay of estriol-16-sulfate (E3-16S) and estriol-3, 16-disulfate (E3-3,16-diS) in maternal serum, cord serum and amniotic fluid at delivery in human pregnancy. Tritiated E3-16S and E3-3,16-diS are added to the fluid being analyzed. The conjugates are separated and purified by sequential chromatography on alumina, Celite and Sephadex LH-20. Each conjugate is hydrolyzed with Glusulase and the released estriol is quantified by radioimmmunoassay. E3-3,16-diS was found in each fluid, most concentrated in the cord serum. Small amounts of E3-16S were found in some amniotic fluids, and this conjugate was virtually absent from the sera. These new estriol conjugates comprise less than 1 percent of total, estriol, apparently too low to be of diagnostic value in human pregnancy.
D000653:Amniotic Fluid / Q000378:metabolism; D001826:Body Fluids / Q000378:metabolism*; D002845:Chromatography; D004964:Estriol / Q000097:blood / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D011247:Pregnancy
D004964:Estriol