Computerized immunoblot analyses (CIBA) of the distribution of prekallikrein and its activation products in vivo and in vitro.
The distribution of prekallikrein and the complexes of kallikrein with C1 inhibitor (C1INH), alpha 2-macroglobulin and approximately 58-kDa protein(s) (e.g. antithrombin III), differs in normal and C1INH-deficient human plasma, activated in vitro, due to different C1INH levels. Different distribution in the deficient plasma activated in vivo or in vitro, suggests different rates of complex clearance.
D000990:Antithrombin III / Q000378:metabolism; D003174:Complement C1 Inactivator Proteins / Q000172:deficiency / Q000378:metabolism; D003201:Computers; D004789:Enzyme Activation; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D066298:In Vitro Techniques; D007610:Kallikreins / Q000378:metabolism; D011288:Prekallikrein / Q000302:isolation & purification* / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism
D003174:Complement C1 Inactivator Proteins; D000511:alpha-Macroglobulins; D000990:Antithrombin III; D011288:Prekallikrein; D007610:Kallikreins