Radioimmunossay for detection of antigen and antibodies to Newcastle disease virus.
When Newcastle disease virus (NDV) is treated with NP-40 and ether a membrane fraction of 150,000 m.w. is obtained. This fraction which is composed of two polypeptides with m.w. of 56,000 and 76,000 was used in a radioimmunoassay (RIA). The assay was developed for both antigen and antibody and was found to be reproducible, specific, and highly sensitive. Titers of 1:51,200 were determined by RIA as compared to 1:4 by agar gel diffusion and 1:200 by hemaglutination inhibition (HI). As little as 5 ng of viral protein were detected by RIA inhibition technique. Labeled antigen could be stored in the presence of serum, KCI and Triton X-100 at -20 degrees C for as long as 6 weeks and retained similar reactivity as fresh reagent.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis* / Q000302:isolation & purification; D011232:Chemical Precipitation; D002645:Chickens; D007106:Immune Sera / Q000032:analysis; D009522:Newcastle disease virus / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera