[Isolation of RNA containing a polyadenylic acid sequence by chromatography on unmodified cellulose].
A new modification of the procedure of the isolation of polyA-containing RNAs is worked out, which makes possible to isolate this RNA fraction free of considerable contamination with rRNA. The administration of 0.0001 M EDTA-Na2 provides the absence of RNA aggregation and prevents non-specific RNA binding on cellulose columns, which takes place when more high EDTA-Na2 concentrations in elution solutions are applied. Under these conditions synthetic polyA in model experiments practically completely binds with cellulose in a broad range of concentrations. It permits to use the procedure described for the preparative isolation of RNA fractions, containing polyA sequences.
D000818:Animals; D001483:Base Sequence; D002482:Cellulose; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D008223:Lymphoma; D051379:Mice; D009374:Neoplasms, Experimental / Q000032:analysis; D012313:RNA / Q000032:analysis*; D012334:RNA, Neoplasm / Q000302:isolation & purification
D012334:RNA, Neoplasm; D012313:RNA; D002482:Cellulose
Vydelenie RNK, soderzhashchikh posledovatel'nosti poliadenilovoĭ kisloty, khromatografieĭ na nemodifitsirovannoĭ tselliuloze