Extrachromosomal inheritance in Schizosaccharomyces pombe. II. Evidence for extrakaryotically inherited respiratory deficient mutants.
In contrast to the wild-type, mutant [ANTr8] is able spontaneously to throw off stable respiratory deficient mutants. The frequency of these mutants is considerably enhanced by treatment with ethidium bromide (EB) or the azo-dye Janus green (JG). An unstable cell state with a petite-like phenotype is found in both mutant [ANTr8] and wild-type after EB-treatment. However, only in the mutant is this unstable cell state followed by the appearance of stable respiratory deficient (RD) mutants. Formation of microcolonies is observed both in [ANTr8] and wild-type. RD mutants were isolated after EB treatment. Three of them (mit-12, mit-25, and mit-30) were analyzed and mit-25 characterized in more detail.
D001203:Ascomycota / Q000378:metabolism*; D004396:Coloring Agents; D004272:DNA, Mitochondrial / Q000378:metabolism*; D004171:Diploidy; D004579:Electron Transport; D004996:Ethidium / Q000494:pharmacology; D005111:Extrachromosomal Inheritance; D006238:Haploidy; D008938:Mitosis; D009154:Mutation; D012568:Schizosaccharomyces / Q000378:metabolism*
D004396:Coloring Agents; D004272:DNA, Mitochondrial; D004996:Ethidium