Two-dimensional electrophoresis analysis of human serum proteins during the acute-phase response.
The serum of patients with meningitis, due to infection by Haemophilus influenzae type b, was analyzed. Several known acute-phase proteins were separated by two-dimensional electrophoresis and estimated quantitatively. In addition, hitherto undescribed reactants were recognized. Gels were calibrated and relevant spots related to master spot numbers in the human serum protein database.
D000209:Acute-Phase Proteins / Q000302:isolation & purification*; D000210:Acute-Phase Reaction / Q000097:blood; D001797:Blood Protein Electrophoresis / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D008583:Meningitis, Haemophilus / Q000097:blood
D000209:Acute-Phase Proteins