Transplantation resistance to mouse ependymoblastoma following immunization with dehistonized syngeneic tumor chromatin.
Female C57BL/J6 mice pretreated with dehistonized ependymoblastoma chromatin rejected 5 x 10(4) or 10(5) s.c.-implanted syngeneic ependymoblastoma cells. Control mice that received Freund's adjuvant containing dehistonized chromatin from normal mouse brain developed palpable tumor nodules in 90% and 35 to 45% of the animals, respectively. Increased binding of spleen lymphocytes from mice pretreated with dehistonized tumor chromatin to Sepharose 4B coupled with components of dehistonized tumor chromatin was observed. This provides initial evidence for the presence of "committed" lymphocytes in mice pretreated with dehistonized chromatin from this mouse ependymoblastoma.
D000818:Animals; D002843:Chromatin / Q000276:immunology* / Q000302:isolation & purification; D004806:Ependymoma / Q000032:analysis / Q000276:immunology*; D005260:Female; D006084:Graft Rejection; D007111:Immunity, Cellular; D007114:Immunization; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology; D013154:Spleen / Q000276:immunology
D002843:Chromatin