title: A simple method of measuring breath hydrogen in carbohydrate malabsorption by end-expiratory sampling.
abstract: 1. A simple method is described for measuring the hydrogen concentration in alveolar air by end-expiratory sampling, by using a modified Haldane-Priestley tube and gas chromatography. Hydrogen was generated in vivo by ingestion of the non-absorbable sugar lactulose. 2. Alveolar hydrogen concentration showed a highly significant correlation with hydrogen production measured either by a rebreathing technique or by a total collection procedure. 3. The coefficient of variation of the end-expiratory method, assessed by comparing sixty-one paired results, was 11-6%. The coefficient of variation in ten measurements in one subject at 1 min intervals was 17-6%.
mesh_terms: D050260:Carbohydrate Metabolism; D002849:Chromatography, Gas; D006801:Humans; D006859:Hydrogen / Q000378:metabolism*; D008722:Methods; D011650:Pulmonary Alveoli / Q000378:metabolism*; D012123:Pulmonary Ventilation
chemical_list: D006859:Hydrogen