Treatment with o,p'-DDD (mitotane) decreased cytochrome P-450, heme, and microsomal protein content in the dog adrenal cortex in vivo.
Treatment with o,p'-DDD, at a does which markedly inhibits steroidogenesis (60 mg/kg, intravenously), significantly decreased adrenal mitochondrial and microsomal "P-450" and microsomal protein contnet 34%, 55%, and 35% respectively, within 3 hours. The reduction of cytochrome P-450 content was accompanied by decrease in heme content, which explains the apparent loss of cytochrome P-450. This action provides a possible explanation for the inhibition of steroidogenesis caused by o,p'-DDD, and indicates that o,p'-DDD initiates a very destructive process in the dog adrenal cortex.
D000302:Adrenal Cortex / Q000648:ultrastructure*; D000311:Adrenal Glands / Q000648:ultrastructure*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004285:Dogs; D006418:Heme / Q000378:metabolism*; D066298:In Vitro Techniques; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D008939:Mitotane / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*
D011506:Proteins; D006418:Heme; D008939:Mitotane; D003577:Cytochrome P-450 Enzyme System