Inorganic polyphosphate as an integral part of alkaline phosphatase preparations.
Alkaline phosphatase (from chicken intestinal sources) was shown to contain a considerable amount of polyanionic phosphorus which was released by basic digestion. The polyanionic phosphorus of alkaline phosphatase is not associated with protein or polyalcohols and does not exhibit a visible or ultraviolet absorption spectrum. Alkaline phosphatase and abiogenic inorganic polyphosphate were found to incorporate 32P-orthophosphate under similar experimental conditions. It has been previously reported that this enzyme will incorporate 32P-orthophosphate into its protein phosphoserine without the apparent concomitant utilization of an energy source. This reported phosphorylation was immediately reversible upon dilution of the phosphorylated enzyme with unlabelled orthophosphate, which indicates that the initial phosphorylation was an exchange reaction. These observations suggest that this polyanionic phosphorus from alkaline phosphatase may be inorganic polyphosphate.
D000469:Alkaline Phosphatase; D000818:Animals; D002645:Chickens; D007422:Intestines / Q000201:enzymology; D010710:Phosphates / Q000032:analysis*; D011108:Polymers / Q000032:analysis*
D010710:Phosphates; D011108:Polymers; D000469:Alkaline Phosphatase