Blood culture technique based on centrifugation: clinical evaluation.
A total of 1,000 blood samples from patients suspected of having a bacteremia were analyzed concurrently, where possible, by three methods: (i) Trypticase soy broth with sodium polyanethol sulfonate and a CO2 atmosphere: (ii) pour plates with either brain heart infusion agar or Sabouraud dextrose agar; and (iii) centrifugation of the suspected organism in a hypertonic solution. There were 176 positive cultures. The centrifugation technique recovered 73% of the positive cultures. The broth and pour plate techniques recovered 38 and 49%, respectively. The centrifugation technique showed an increased isolation rate for Pseudomonas, fungi, and gram-positive cocci. In general, for each organism the time required for the detection of a positive culture was shortest for the centrifugation technique.
D001419:Bacteria / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000382:microbiology*; D001769:Blood / Q000382:microbiology*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D005069:Evaluation Studies as Topic; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D006982:Hypertonic Solutions; D009181:Mycoses / Q000382:microbiology*; D013045:Species Specificity
D006982:Hypertonic Solutions