A protein factor required for activation of phosphoenolpyruvate carboxykinase by ferrous ions.
When rat liver cytosolic P-enolpyruvate carboxykinase is purified, its activity is no longer enhanced by incubation with 30 muM Fe2+. Ferrous ion stimulation of the purified enzyme is restored by the addition of rat liver cytosol. The agent responsible is a cytosolic protein, named P-enolpyruvate carboxykinase ferroactivator, that was readily separated from the enzyme during purification of the latter. A quantitative assay for P-enolpyruvate carboxykinase ferroactivator is described. Subcellular fractionation of livers from fasted rats shows that 98% of the combined mitochondrial and cytosolic P-enolpyruvate carboxykinase ferroactivator activity resides in the cytosol. Fasting does not produce significant change in this cytosolic activity when compared to that of fed animals. Examination of various tissue homogenates shows that the ferroactivator is found in liver, kidney, erythrocytes, adipose tissue, and brain. No activity was detected in blood serum or skeletal muscle. The ability to enhance the activity of purified rat liver cytosolic P-enolpyruvate carboxykinase in the presence of Fe2+ is not species specific. P-enolpyruvate carboxykinase ferroactivator may have an important function in regulating enzyme activity in vivo.
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D004789:Enzyme Activation; D004912:Erythrocytes / Q000378:metabolism; D005215:Fasting; D005296:Ferrous Compounds / Q000494:pharmacology*; D007501:Iron / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D008345:Manganese / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism*; D011506:Proteins / Q000494:pharmacology*; D051381:Rats
D005296:Ferrous Compounds; D010710:Phosphates; D011506:Proteins; D008345:Manganese; D007501:Iron; D010729:Phosphoenolpyruvate Carboxykinase (GTP)