title: Enrichment and isolation of nitrogen fixing hydrogen bacteria.
abstract: An enrichment method for nitrogen fixing hydrogen bacteria is described. The procedure invariably resulted in the isolation of yellow-pigmented coryneform bacterial strains assigned to Corynebacterium autotrophicum. The procedure included a serial transfer in an ammonium-free mineral liquid medium under an atmosphere of 10% hydrogen, 5% oxygen, 10% carbon dioxide and 75% nitrogen, followed by a short alkali treatment and by streaking on nutrient broth-succinate agar. The ability to fix nitrogen was confirmed by the acetylene reduction test and by 15N2 incorporation.
mesh_terms: D000114:Acetylene / Q000378:metabolism; D003352:Corynebacterium / Q000302:isolation & purification* / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
chemical_list: D006601:Hexoses; D013395:Sucrose; D006859:Hydrogen; D000114:Acetylene