Anti-actomyosin. Influence on adhesive behaviour of eukaryotic cells and of Cuvierian tubules.
The effect of antisera against chicken gizzard smooth-muscle actomyosin and against pectoralis striated-muscle actomyosin on adhesive behaviour of eukaryotic cells (from sea urchin embryos and from a silicious sponge) and of Cuvierian tubules has been studied. The results with sea urchin cells, which require divalent cations for aggregation, showed that antiserum to chicken gizzard smooth-muscle actomyosin inhibited reaggreagation of trypsin-treated cells better than mechanically dissociated cells, while anti-chicken pectoralis striated-muscle had no effect. Primary reaggreagation of trypsin-dissociated sponge cells, in the presence of calcium and magnesium, is also inhibitable by anti-gizzard smooth-muscle but not by anti-pectoralis straited muscle. Anti-gizzard smooth-muscle had no effect on secondary reaggregation of sponge cells mediated by a soluble aggregation factor. Anti-gizzard smooth-muscle inhibited Cuvierian tubule adhesion.
D000205:Actomyosin / Q000276:immunology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002645:Chickens; D004625:Embryo, Nonmammalian; D005260:Female; D005895:Gizzard, Avian / Q000276:immunology; D007106:Immune Sera; D009130:Muscle, Smooth / Q000276:immunology; D009132:Muscles / Q000276:immunology; D011161:Porifera / Q000166:cytology / Q000502:physiology*; D012617:Sea Urchins / Q000166:cytology / Q000502:physiology*
D007106:Immune Sera; D000205:Actomyosin; D002118:Calcium