Decondensation of human sperm nuclei and HP1 protamine degradation from normospermia and asthenospermia in Xenopus egg extracts.
The process of human sperm decondensation has been studied in vitro in cytoplasmic extracts prepared from unfertilized Xenopus laevis eggs. The chromatin decondensation-recondensation cycle was divided into four stages according to chromatin appearance. Spermatozoa from normospermia and asthenospermia were evaluated according to their capacity to reach these stages, and their DNA integrity was assessed by acridine orange (AO) staining. We observed a significant difference between normospermia and asthenozoospermia in the ability to achieve the cycle of chromatin decondensation-recondensation. These results correlated with AO staining. The role of human protamine 1 degradation in the decondensation process was evaluated by immunostaining. It was found not to be a prerequisite for the earlier stage of chromatin decondensation and it was not implied in the latest stages of pronuclear development.
D000165:Acridine Orange; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D002843:Chromatin / Q000648:ultrastructure*; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D010063:Ovum / Q000378:metabolism*; D011479:Protamines / Q000378:metabolism*; D013094:Spermatozoa / Q000737:chemistry / Q000648:ultrastructure*; D013194:Staining and Labeling; D014982:Xenopus laevis
D002843:Chromatin; D011479:Protamines; D004247:DNA; D000165:Acridine Orange