A specific gas chromatographic method for the determination of microsomal styrene monooxygenase and styrene epoxide hydratase activities.
A gas chromatographic (GC) method for the determination of the metabolite resulting from the activities of microsomal styrene monooxygenase (epoxide synthetase) and epoxide hydratase using styrene or styrene epoxide as substrates has been developed. The determination of the activities of both enzymes is based on the GC determination of phenylethylene glycol after its esterification with n-butylboronic acid. Kinetic parameters for both enzymes are given.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis*; D002849:Chromatography, Gas; D004851:Epoxide Hydrolases / Q000032:analysis*; D007700:Kinetics; D008722:Methods; D008861:Microsomes / Q000201:enzymology*; D013343:Styrenes
D013343:Styrenes; D001189:Aryl Hydrocarbon Hydroxylases; D004851:Epoxide Hydrolases