title: The derivatization of oxidized polysaccharides for protein immobilization and affinity chromatography.
abstract: The present report describes the preparation of modified polysaccharides matrices useful for the synthesis of affinity adsorbents and immobilized proteins. Hydrazido-matrices were synthesized by condensing an excess of the bifunctional reagent, adipic acid dihydrazide, with periodate oxidized cellulose paper, Sephadex, or Sepharose matrices. Ribonucleotide dialdehyde cofactors, glyceraldehyde 3-phosphate, pyridoxal 5'-phosphate and oxidized DNAase B were separately bound to the hydrazido-polymers. Azido-matrices obtained by modification of the hydrazido-derivatives were coupled to specific amino ligands such as amino acids and proteins. Several adsorbents were prepared and used as models for affinity chromatography.
mesh_terms: D000447:Aldehydes / Q000138:chemical synthesis; D001386:Azides / Q000138:chemical synthesis; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D002918:Chymotrypsin / Q000302:isolation & purification; D006834:Hydrazines / Q000138:chemical synthesis; D008024:Ligands; D008722:Methods; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000138:chemical synthesis*; D011506:Proteins / Q000302:isolation & purification*; D012265:Ribonucleotides / Q000138:chemical synthesis; D012685:Sepharose; D014357:Trypsin / Q000302:isolation & purification; D014361:Trypsin Inhibitors
chemical_list: D000447:Aldehydes; D001386:Azides; D006834:Hydrazines; D008024:Ligands; D011134:Polysaccharides; D011506:Proteins; D012265:Ribonucleotides; D014361:Trypsin Inhibitors; D012685:Sepharose; D002918:Chymotrypsin; D014357:Trypsin