A new iron-binding protein isolated from intestinal mucosa.
A new iron-binding protein contained in guinea pig intestinal mucosa has been purified to homogeneity. The protein has a molecular weight of 78,000 in a nondissociating system and 43,000 in SDS-gel electrophoresis. It binds approximately 2 moles of iron per mole with a formation constant of 10(19) at pH 7. It is distinguished from transferrin and lactoferrin by differences on DEAE-Sephadex chromatography and spectroscopy and by its failure to cross-react with antisera to these other iron-binding proteins.
D000818:Animals; D005260:Female; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007781:Lactoferrin / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism
D011506:Proteins; D014168:Transferrin; D007501:Iron; D007781:Lactoferrin