title: Specific types of prosomes are associated to subnetworks of the intermediate filaments in PtK1 cells.
abstract: Prosomes are small ribonucleoprotein (RNP) particles of unique morphology in the electron microscope but of variable protein and RNA composition, depending on the differentiation state of the cells studied. They were initially observed as subcomplexes of untranslated mRNP. In previous studies, we found that prosomes are associated to the intermediate filaments (IF) of cytokeratin type in HeLa and PtK1 cells. Here we have studied in detail the association of prosomal antigens with the IF networks in PtK1 cells. Contrary to our earlier conclusions, in these cells the vimentin fibers also carry prosomes which, thus, distribute in between the two types of networks. During the selective collapse of the IF induced by acrylamide, and upon recovery after the withdrawal of the drug, no dissociation of the prosome and IF networks of cytokeratin- and vimentin-type could be observed. These data show that even in a dynamic situation, prosome and IF antigens do not dissociate, indicating strongly that they are located on one and the same structure. Furthermore, the differential distribution of specific prosomal antigens between both types of intermediate filament networks indicates that prosomes do not ubiquitously populate the intermediate filaments but occupy subnetworks of either vimentin or cytokeratin type.
mesh_terms: D020106:Acrylamide; D000178:Acrylamides / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D004173:Dipodomys; D007122:Immunoelectrophoresis; D007382:Intermediate Filaments / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D007633:Keratins / Q000378:metabolism; D007668:Kidney; D008870:Microtubules / Q000187:drug effects; D008970:Molecular Weight; D012261:Ribonucleoproteins / Q000737:chemistry*; D014746:Vimentin / Q000378:metabolism
chemical_list: D000178:Acrylamides; D012261:Ribonucleoproteins; D014746:Vimentin; D020106:Acrylamide; D007633:Keratins