Thrombin-induced oxygen consumption, malonyldialdehyde formation and serotonin secretion in human platelets.
The relationship of a thrombin-induced burst in O2 consumption to lipid peroxidation in washed human platelets was investigated by measuring malonyldialdehyde, a by-product of endoperoxide degradation in platelets. The ratio of O2 consumed by malonyldialdehyde produced was approximately 7:1. Acetylsalicylate blocked the formation of malonyldialdehyde completely and partially inhibited the O2 burst induced by thrombin. 5,8,11,14-Eicosatetraynoic acid inhibited the O2 burst as well as the malonyldialdehyde formation completely. The release of [14C]serotonin was not affected by either inhibitor.
D001792:Blood Platelets / Q000378:metabolism*; D006801:Humans; D008314:Malonates / Q000097:blood*; D008315:Malondialdehyde / Q000096:biosynthesis / Q000097:blood*; D010101:Oxygen Consumption / Q000187:drug effects*; D012701:Serotonin / Q000097:blood*; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D008314:Malonates; D012701:Serotonin; D008315:Malondialdehyde; D013917:Thrombin