Micro-scale two-dimensional polyacrylamide gell electrophoresis of ribosomal proteins.
A specially designed apparatus and conditions are described for the rapid analysis of ribosomal proteins by two-dimensional gel electrophoresis on a micro scale. The resolution of proteins in electropherograms is comparable to that obtained with other systems, but because of miniaturization, only 0.5 to 1 mug of each protein is required, and the entire procedure, including electrophoresis in both dimensions, and staining and destaining can be completed in 6 to 7 hours.
D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation*; D008722:Methods; D008904:Miniaturization / Q000295:instrumentation; D012269:Ribosomal Proteins / Q000032:analysis*; D013997:Time Factors
D012269:Ribosomal Proteins