[The effect of insulin therapy on the level of natural killer cells of different immunological phenotypes (CD16+, CD56+ and CD57+) in the blood of patients with diabetes mellitus type I].
Flow cytometry with monoclonal antibody Leu-7 (CD57), Leu-11 (CD56) and Leu-19 (CD56) were used to study the content of different subpopulations of natural killer cells (NK-cells) in the blood of type I diabetes mellitus patients before and after insulin treatment and in healthy people. After a course of insulin therapy most patients showed restoration of the total cell number with antigens on their surface characteristic of NK-cells, especially CD56, that indicates the essential role of hypoinsulin in the depression of the NK-system. At the same time a small group of patients was distinguished who did not show such normalization. This may indicate participation of other mechanisms in the formation of natural immunity failure, in particular, the genetic.
D015703:Antigens, CD / Q000187:drug effects* / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000187:drug effects* / Q000276:immunology; D019002:CD56 Antigen; D018959:CD57 Antigens; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007328:Insulin / Q000627:therapeutic use*; D049528:Insulin, Long-Acting / Q000627:therapeutic use*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D007958:Leukocyte Count / Q000187:drug effects; D010641:Phenotype; D017452:Receptors, IgG / Q000187:drug effects* / Q000276:immunology
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D019002:CD56 Antigen; D018959:CD57 Antigens; D007328:Insulin; D049528:Insulin, Long-Acting; D017452:Receptors, IgG
Vliianie insulinoterapii na soderzhanie estestvennykh kletok-killerov razlichnogo immunologicheskogo fenotipa (CD16+, CD56+ i CD57+) v krovi bol'nykh sakharnym diabetom I tipa.