Random distribution of centromere regions at mitosis in cultured cells of Muntiacus muntjak.
The manner in which centromere regions of mitotic chromosomes are distributed with respect to the age of their DNA was studied. Cells of the Indian deer Muntiacus muntjak, were grown in the presence of bromodeoxyuridine (BrdU) for two generations and stained with the fluorescent dye Hoechst 33258. Chromatids containing "granddaughter DNA" appear dim when compared with those containing "grandparental DNA". The frequencies of the various anaphase patterns of bright and dim centromere regions were binomially distributed, indicating random distribution of chromatids with respect to the age of their DNA templates.
D000818:Animals; D002478:Cells, Cultured; D002842:Chromatids; D003670:Deer; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008938:Mitosis; D013045:Species Specificity; D013997:Time Factors