Holding plastic-embedded specimens for sectioning in a rotary microtome.
Three methods are evaluated for holding capsules of plastic-embedded tissue for rotary microtomy. Use of a V-block is rapid but deforms the capsule. Gluing the capsule to a supporting block is useful for reorientation of the capsule but otherwise time consuming. adapter is easy to use, does not deform the tissue, and is the preferred method for routine microtomy.
D000818:Animals; D008099:Liver / Q000033:anatomy & histology; D008867:Microtomy / Q000379:methods*; D010969:Plastics; D051381:Rats
D010969:Plastics