Cellular control of the tick-borne virus antigen production in persistently infected cell culture.
The influence of inhibition or stimulation of cellular DNA synthesis on tick-borne virus antigen production in persistently infected cell culture was studied. Either mitomycin C or cytosine-arabinoside caused cessation of antigen-containing cell number increase. Stimulation of cellular DNA synthesis by growth medium change increased the level of antigen-containing cells. When HEp-2-Sof culture was synchronized, a correlation was observed between the entrance of cells into DNA synthesis phase and the increase of proportion of antigen-containing cells.
D000956:Antigens, Viral; D002460:Cell Line; D003470:Culture Media; D003561:Cytarabine / Q000494:pharmacology*; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D008937:Mitomycins / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism
D000956:Antigens, Viral; D003470:Culture Media; D008937:Mitomycins; D003561:Cytarabine; D013936:Thymidine