[Sedimentation properties of virion RNA of Newcastle disease virus after annealing under different conditions].
After annealing at high temperatures 50S RNA of Newcastle disease virus was partially degraded. The resulting fragments had the sedimentation coefficient of 23--32S. Solution of dimethylsulfoxide and formamide were used to decrease the temperature of annealing. However, annealing with denaturating agents caused agglomeration of 50S RNA molecules. This phenomenon is discussed.
D002499:Centrifugation, Density Gradient; D004121:Dimethyl Sulfoxide; D006358:Hot Temperature; D009522:Newcastle disease virus; D012367:RNA, Viral
D012367:RNA, Viral; D004121:Dimethyl Sulfoxide
Sedimentatsionnye svoĭstva virionnoĭ RNK virusa bolezni N'iukasla posle otzhiga pri razlichnykh usloviiakh