Sequence of methylated nucleotides at the 5'-terminus of adenovirus-specific RNA.
RNA labeled with [methyl-3H] methionine and [14C]uridine was isolated from the cytoplasm of adenovirus-infected cells and purified by poly(U)-Sepharose chromatography and hybridization to filters containing immobilized adeovirus DNA. Analysis by dimethyl sulfoxide-sucrose gradient sedimentation suggested that the major mRNA species were methylated. 7-Methylguanosine was identified at the 5'-terminus of the advenovirus-specific RNA and could be removed by periodate oxidation and beta-elimination. Structures of the type m7G(5')ppp(5')Nm containing the unusual nucleoside N6, O2'-dimethyladenosine, and smaller amounts of 2'-O-methyladenosine were isolated by DEAE-cellulose chromatography after P1 nuclease digestion of the RNA. Evidence for some 5'-terminal sequences, m7G(5')ppp(5')m6AmpNm, with additional 2'-O-methylribonucleosides was also obtained. A base-methylated nucleoside, N6-methyladenosine, is located within the RNA chain and is released as a mononucleotide by alkali hydrolysis.
D000241:Adenosine / Q000031:analogs & derivatives / Q000032:analysis; D000256:Adenoviridae / Q000032:analysis*; D003593:Cytoplasm / Q000032:analysis; D004279:DNA, Viral; D006151:Guanosine / Q000031:analogs & derivatives / Q000032:analysis; D006367:HeLa Cells; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*
D004279:DNA, Viral; D009711:Nucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D006151:Guanosine; D000241:Adenosine