The properties of oligonucleotide fragments containing the triphosphorylated 5'-termini of nuclear pre-mRNA from Ehrlich ascites carcinoma cells.
Triphosphorylated 5'-end fragments 50-150 nucleotides in length were isolated from nuclear pre-mRNA with the aid of a hydroxyapatite chromatography. They are enriched in U and G (28 and 26%, respectively). About 15% of the fragments isolated from poly(U)+RNA contain poly(U) tracks. Neither poly(A)- nor double-stranded sequences were found. Hybridization experiments in conditions of vast DNA excess demonstrated that the 5'-end fragments contain a low amount of highly repetitive sequences but enriched in sequences hybridizing at C0t 1/2 approximately 100.
D000818:Animals; D001483:Base Sequence; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009843:Oligoribonucleotides; D011072:Poly U / Q000032:analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D012265:Ribonucleotides / Q000032:analysis
D009843:Oligoribonucleotides; D012333:RNA, Messenger; D012265:Ribonucleotides; D011072:Poly U