Determination of risperidone and 9-hydroxyrisperidone in plasma, urine and animal tissues by high-performance liquid chromatography.
A high-performance liquid chromatographic method has been developed for the simultaneous determination of the new antipsychotic risperidone and its major metabolite 9-hydroxyrisperidone in plasma, urine and animal tissues. The alkalinized plasma samples were extracted with ethyl acetate and further purified prior to reversed-phase chromatography with ultraviolet detection at 280 nm. The method could also be applied to urine samples and animal tissue homogenates. Quantification limits were 2 ng/ml for plasma and urine and 10 ng/g for animal tissue. The method was applied to pharmacokinetic studies in experimental animals, human volunteers and patients.
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007555:Isoxazoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D000068882:Paliperidone Palmitate; D010880:Piperidines / Q000032:analysis* / Q000097:blood / Q000652:urine; D011743:Pyrimidines / Q000032:analysis* / Q000097:blood / Q000652:urine; D011786:Quality Control; D015203:Reproducibility of Results; D018967:Risperidone; D013056:Spectrophotometry, Ultraviolet
D007555:Isoxazoles; D010880:Piperidines; D011743:Pyrimidines; D018967:Risperidone; D000068882:Paliperidone Palmitate