Urogenital infection and seminal excretion after inoculation of bulls and rams with chlamydiae.
Five mature rams and 4 bulls were inoculated parenterally with bovine or ovine chlamydial strains of type 1 and 2. One to 3 days later, all animals developed a chlamydemia lasting 4 to 8 days. Chlamydial agents were isolated from the semen near the end of the chlamydemic phase. All rams and 3 of 4 inoculated bulls excreted chlamydiae in the semen for 22 to 29 days. From 8 to 39 days after inoculation, selected rams or bulls were killed to test for chlamydial infection in the urogenital tract and other organs. Chlamydiae were isolated in developing chicken embryos from testis, epididymis, and accessory sex glands. Bulls examined 29 and 39 days after inoculation did not harbor chlamydiae. Chlamydiae were also not isolated from 3 control bulls which were from the same herd as the principal bulls. All inoculated bulls and rams had a group-specific chlamydial antibody response within 7 days. The titers reached maximal levels of 128 to 512 at 14 days after inoculation. Subsequently, the antibody titers decreased gradually. Seminal plasma collected at different times after animals were inoculated did not fix complement in the presence of chlamydial group antigen. The number of polymorphonuclear leukocytes in the semen increased during the experiment. The semen was grossly purulent in 2 rams inoculated with the type 2 chlamydial strain of polyarthritis.
D000818:Animals; D000917:Antibody Formation; D001769:Blood / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology*; D002689:Chlamydia / Q000302:isolation & purification; D002690:Chlamydia Infections / Q000276:immunology / Q000662:veterinary*; D005832:Genital Diseases, Male / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D012661:Semen / Q000382:microbiology*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology*; D013737:Testis / Q000382:microbiology