Factors involved in concanavalin A agglutination of KB and NC37 cells grown in suspension culture.
NC37 and KB cells grown in suspension culture remained agglutinable at 23 degrees C by concanavalin A (Con A) after sufficient glutaraldehyde fixation to prevent lateral mobility (clustering) of Con A binding sites. In contrast, at 4 degrees C agglutination of the fixed cells was blocked. No significant differences in 3H-Con A binding were observed between unfixed cells and glutaraldehyde-fixed cells at 23 degrees C and unfixed cells at 4 degrees C. Con A bound to fixed cells at 4 degrees C produced agglutination when the cells were washed and then warmed to 23 degrees C. Thus a cold-sensitive factor unrelated to the binding of Con A or the clustering of Con A sites is necessary for agglutination to occur.
D001665:Binding Sites; D002449:Cell Aggregation / Q000187:drug effects*; D002460:Cell Line; D003208:Concanavalin A / Q000378:metabolism / Q000494:pharmacology*
D003208:Concanavalin A