title: Preparation of 1,2-3H-21-deoxycortisol by incubation of 1,2-3H-17-hydroxyprogesterone with rat adrenal mitochondria.
abstract: A method is described for preparation of 1,2-3H-21-deoxycortisol by incubation of 1,2-3H-17-hydroxyprogesterone with rat adrenal mitoochondria. The radiochemical purity of the product was established by two subsequent chromatographies, reversed isotopic dilution and oxidation to 21-deoxycortisone. The final yield of 1,2-3H-21-deoxycortisol was approximately 15%.
mesh_terms: D015065:17-Hydroxycorticosteroids / Q000031:analogs & derivatives*; D000311:Adrenal Glands / Q000648:ultrastructure*; D000818:Animals; D003350:Cortodoxone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000302:isolation & purification; D003460:Crystallization; D005260:Female; D006900:Hydroxylation; D006908:Hydroxyprogesterones / Q000378:metabolism*; D066298:In Vitro Techniques; D008928:Mitochondria / Q000378:metabolism*; D051381:Rats; D014316:Tritium
chemical_list: D015065:17-Hydroxycorticosteroids; D006908:Hydroxyprogesterones; D014316:Tritium; C003556:21-deoxycortisol; D003350:Cortodoxone