Immunological cross-reaction between an Onchocerca paramyosin-like molecule and a microfilaria surface antigen.
A monoclonal antibody (2A5B9), previously shown to be reactive with a 14 kD surface associated antigen of Onchocerca microfilariae, was found to recognise a 92 kD molecule present in an adult worm extract. The antibody was used to select cDNA clones with a coding capacity larger than 14 kD, from a lambda gt11 library of O. volvulus. Nucleotide sequencing of the cDNA of one such clone revealed extensive homology to the myosin (unc-54) and paramyosin (unc-15) genes of Caenorhabditis elegans, similarly to myosin and paramyosin genes of Onchocerca volvulus, Brugia malayi, Dirofilaria immitis and Schistosoma mansoni. The immunological implications of antigenic cross-reactivity between a surface molecule and paramyosin, a known protective antigen, are discussed.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D003429:Cross Reactions; D004247:DNA / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D015723:Gene Library; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D008297:Male; D008842:Microfilariae / Q000276:immunology; D008969:Molecular Sequence Data; D009218:Myosins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D009854:Onchocerca / Q000235:genetics / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D014335:Tropomyosin / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000954:Antigens, Surface; D000939:Epitopes; D015801:Helminth Proteins; D014335:Tropomyosin; C078794:paramyosin-like antigen, Onchocerca volvulus; D004247:DNA; D009218:Myosins