title: Effect of cerium on the rat liver: an ultrastructural and biochemical study.
abstract: In rats, liver steatosis and necrosis were induced by cerous chloride (CeCl3) and the evolution of these changes was examined. By electron microscopy, 17 hours after CeCl3 treatment, dilation, disorganization and degranulation of the rough endoplasmic reticulum (RER) were noted with an increase in the number and electron density of lysosome-like bodies. In addition, nuclear chromatin showed showed a marked focal electron density, and the nuclear membrane appeared to be interrupted. At 24 hours, the RER was markedly dilated and degranulated, with free ribosomes aggregated in the cytoplasm. The Golgi cisternae appeared to be empty. There was an increase in the number and size of lipid droplets, with depletion of glycogen. At 48 hours, a massive proliferation of smooth endoplasmic reticulum (SER) vesicles occurred. Large lipid droplets were scattered throughout the cytoplasm, while the mitochondria displayed mild changes. By the 8th day, the number of lipid droplets returned to normal; no abnormalities were detected in the other cell organelles. Biochemically, the total hepatic ATP levels fell significantly by the 12th hour, dropping to a minimum by the 48th hour. The liver was gradually depleted of glycogen within the first 48 hours, while hepatic triglycerides increased rapidly, reaching a peak at 96 hours. Exogenous administration of adenine, ATP (adenosine triphosphate), or tryptophan completely prevented CeCl3-induced mortality; hepatic fat accumulation and necrosis were markedly decreased. Glucose, dl-methionine, and choline had no protective effect. It appears that a defect in hepatocellular lipoprotein synthesis and/or release may be responsible for lipid accumulation.
mesh_terms: D000225:Adenine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D002467:Cell Nucleus / Q000648:ultrastructure; D002563:Cerium / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005234:Fatty Liver / Q000139:chemically induced; D005260:Female; D006056:Golgi Apparatus / Q000648:ultrastructure; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008112:Liver Glycogen / Q000378:metabolism; D008928:Mitochondria / Q000648:ultrastructure; D051381:Rats; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism; D014364:Tryptophan / Q000494:pharmacology
chemical_list: D008112:Liver Glycogen; D014280:Triglycerides; D002563:Cerium; D014364:Tryptophan; D000255:Adenosine Triphosphate; D000410:Alanine Transaminase; D000225:Adenine; D001663:Bilirubin