title: Derivatives of the insect moulting hormone for affinity chromatography, and their biological activities.
abstract: Several derivatives of the arthropod moulting hormone have been synthesized which were coupled to AH Sepharose 4B yielding about 2 mumole ligand per g wet gel. As an indication of the suitability of the ligands for biological work the puff inducing capacity of their methyl esters was tested. The methyl ester of inokosterone-C-26-carboxylic acid possesses the highest biological activity; lower activities were obtained with the esters of ecdysterone-C-6-CM-oxime and ecdysterone hemisuccinates. Therefore, inokosterone-C-26-carbocylic acid should be a useful ligand for affinity chromatography of ecdysone recptors from insect tissues.
mesh_terms: D002782:Cholestenes / Q000031:analogs & derivatives / Q000494:pharmacology; D002846:Chromatography, Affinity; D004330:Drosophila / Q000378:metabolism; D004441:Ecdysterone / Q000031:analogs & derivatives / Q000494:pharmacology; D008024:Ligands; D011485:Protein Binding; D012469:Salivary Glands / Q000378:metabolism; D012685:Sepharose
chemical_list: D002782:Cholestenes; D008024:Ligands; D004441:Ecdysterone; D012685:Sepharose