Chromosomal anomalies in radiation-induced fibrosis in the pig.
R-banded karyotypes were established on fibroblasts from fibrotic tissues derived from experimental fibrosis induced in pigs, either surgically or by 64 Gy of gamma-rays from iridium-192. No chromosome aberrations were observed in the surgical fibrosis. In radiation-induced fibrosis, the high frequency of abnormal karyotypes and the frequent complexity of the chromosomal rearrangements suggest that the fibroblasts originated either from the 64-Gy area, or from the penumbra, but certainly not from non-irradiated areas. At early passages in vitro, almost all karyotypes were different, demonstrating a multiclonal origin of fibrotic tissue. At late passages (above 24), the situation was quite different, with the persistence of one or two clones only, demonstrating a strong selective pressure occurring in vitro.
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D005260:Female; D005355:Fibrosis / Q000209:etiology / Q000235:genetics*; D005720:Gamma Rays / Q000009:adverse effects; D007621:Karyotyping; D008297:Male; D011833:Radiation Injuries, Experimental / Q000235:genetics*; D012867:Skin / Q000473:pathology / Q000528:radiation effects*; D013552:Swine