Chromatography of biopolymers and their fragments on ion-exchange derivatives of the hydrophilic macroporous synthetic gel spheron.
New ion-exchange materials have been developed from the synthetic glycol methacrylate gel Spheron: carboxymethyl-Spheron (weakly acidic); phosphonyl-Spheron (medium acidic); sulphonyl-Spheron (strongly acidic); diethylaminoethyl-Spheron (Type 1, weakly basic; Type 2, medium to strongly basic). Information is presented on the chromatographic characteristics of these new, macroporous, hydrophilic and rigid ion-exchangers as determined by experiments on the separation of mixtures of proteins, peptides, amino acids, nucleic acids, oligonucleotides, and nucleotides. The possibility is discussed of the use of ion-exchangers of this type in high-performance liquid chromatography of biopolymers.
D000596:Amino Acids; D001704:Biopolymers; D002850:Chromatography, Gel; D006018:Glycols; D007475:Ion Exchange Resins; D046911:Macromolecular Substances; D008689:Methacrylates; D009696:Nucleic Acids; D009711:Nucleotides; D010455:Peptides; D011506:Proteins
D000596:Amino Acids; D001704:Biopolymers; D006018:Glycols; D007475:Ion Exchange Resins; D046911:Macromolecular Substances; D008689:Methacrylates; D009696:Nucleic Acids; D009711:Nucleotides; D010455:Peptides; D011506:Proteins