Evidence that the isomerization of D-ribose and L-rhamnose is catalyzed by the same enzyme in Mycobacterium smegmatis.
D-Ribose isomerase was purified and crystallized from cells of Mycobacterium smegmatis grown on either D-ribose or L-rhamnose. Isomerase activity for both of these sugars remained together throughout the purification. The isomerase from L-rhamnose-grown cells had the same chemical and physical properties as the enzyme isolated from D-ribose grown cells. In addition, immunological studies indicated that both activities were in the same protein since antisera prepared against either of the crystals cross-reacted with the other and gave lines of symmetry by the agar gel diffusion method.
D002238:Carbohydrate Epimerases / Q000276:immunology / Q000378:metabolism*; D003429:Cross Reactions; D009161:Mycobacterium / Q000201:enzymology* / Q000378:metabolism; D012210:Rhamnose / Q000378:metabolism*; D012266:Ribose / Q000378:metabolism*; D013237:Stereoisomerism
D012266:Ribose; D002238:Carbohydrate Epimerases; D012210:Rhamnose