The storage of cow eggs at room temperature and at low temperatures.
The survival and development of cow eggs in the rabbit oviduct after storage at room temperature and after cooling and storage at 0-7-5 degrees C was examined. In PBS medium at room temperature 88% of Day-5 and 85% of Day-3 eggs showed normal development, but in TCM 199, 71% of Day-5 and only 49% of Day-3 eggs showed normal development. Duration of storage (1 1/2-2 hr or 6 1/2-7 1/2 hr) and cleavage stage before storage had no appreciable effect on development. Some retardation of development occurred in Day-3 eggs after 96 hr in the rabbit oviduct when compared to Day-5 eggs after 48 hr. Cooling of Day-5 and Day-6 eggs to 0-7-5 degrees C resulted in degeneration of a large proportion of eggs. Of the factors examined, storage medium (PBS or PBS+20%FCS), storage time (2 min, 24 hr) and storage temperature (0, 2, 5 or 7-5 degrees C) had little effect, but slower cooling rates tended to improve survival of eggs although the differences were not significant. More morulae (greater than 32 cells) than 8-to 24-celled eggs developed normally.
D000818:Animals; D001755:Blastocyst / Q000648:ultrastructure; D002417:Cattle; D003080:Cold Temperature / Q000009:adverse effects; D003470:Culture Media; D005260:Female; D010063:Ovum / Q000648:ultrastructure; D011247:Pregnancy; D011817:Rabbits; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation; D014183:Transplantation, Heterologous; D015053:Zygote / Q000637:transplantation*
D003470:Culture Media