Message activity of influenza viral RNA.
The message activity of influenza virion RNA in the wheat germ cell-free protein-synthesizing system was investigated. RNA extracted from purified virions was found to direct the synthesis of a polypeptide that had the mobility of viral nucleocapsid protein on sodium dodecyl sulfate-polyacrylamide gels. Further characterization of the protein indicated it was not the nucleocapsid protein. No other polypeptides were detected. We conclude that influenza virion RNA is inactive as a template for the synthesis of virus-specific proteins.
D000596:Amino Acids / Q000378:metabolism; D002474:Cell-Free System; D009975:Orthomyxoviridae / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010936:Plant Extracts; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D013698:Templates, Genetic; D014908:Triticum; D014764:Viral Proteins / Q000096:biosynthesis*
D000596:Amino Acids; D010936:Plant Extracts; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins