Subcellular distribution of S-adenosylmethionine decarboxylase in rat liver. Evidence of decarboxylation of S-adenosylmethionine separate from synthesis of spermidine.
The activity of S-adenosylmethionine decarboxylase in rat liver homogenates is localized chiefly in the crude nuclear fraction, probably associated with membrane fragments, with the remainder in the supernatant fraction. This distribution is not paralleled by the activity of the cytoplasmic enzyme, lactate dehydrogenase. The spermidine-synthesizing activity of whole homogenate is recovered entirely in the supermidine-synthesizing activity of whole homogenate is recovered entirely in the supernatant fraction. Measurement of various kinetic parameters in crude fractions provided not positive evidence for isozymes of S-adenosylmethionine decarboxylase. Some species do not possess a sedimentable fraction of S-adenosylmethionine decarboxylase activity in liver. In those species all activity present in the whole homogenate of liver is released into the supernatant fraction.
D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002262:Carboxy-Lyases / Q000378:metabolism*; D002645:Chickens; D003653:Decarboxylation; D005289:Ferrets; D005849:Gerbillinae; D006168:Guinea Pigs; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D011700:Putrescine / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012436:S-Adenosylmethionine / Q000378:metabolism*; D013045:Species Specificity; D013095:Spermidine / Q000096:biosynthesis* / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013154:Spleen / Q000201:enzymology; D013347:Subcellular Fractions / Q000378:metabolism
D013096:Spermine; D012436:S-Adenosylmethionine; D002262:Carboxy-Lyases; D012437:Adenosylmethionine Decarboxylase; D013095:Spermidine; D011700:Putrescine