High-performance liquid chromatographic determination of kanamycin.
A fast, selective, and precise liquid chromatographic method for simultaneous, independent determination of kanamycins A and B is described. Sample components are separated on a pellicular cation exchanger and monitored by fluorescence using post-column on-line derivatization. Less than 0.35 mug of kanamycin B can be detected in as much as 7 mug kanamycin A injected. The detection limit for kanamycin A is less than 20 ng injected. Reproducibility of the entire chromatographic system is about 1% (2 theta) based upon repeated injections of standards. Precision of repeated process sample preparation is about 6% (2 theta). Chromatographic analysis time is less than 15 min per sample.
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005450:Fluorescamine; D007612:Kanamycin / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013696:Temperature
D005450:Fluorescamine; D007612:Kanamycin