Haemopoietic progenitor cells in prenatal congenitally anaemic 'flexed-tailed' (f/f) mice.
The incidences of erythroid colony forming cells (CFUe) and granulocyte-macrophage colony forming cells (CFUc) have been measured in 11-18 d prenatal livers of mice of genotype f/f and nearly congenic +/+ controls. In normal fetal livers numbers of CFUe (cells able to form colonies of 16 or more cells after 72 h in vitro) rise to a maximum on day 14 of gestation and represent c 1% of total fetal liver cells. In f/f fetal livers, peak values for numbers and proportions of CFUe are 50% of normal. The f/f lesion does not reduce the numbers of CFUc in fetal liver. Since this deficiency in CFUe parallels deficiencies of similar magnitude in spleen-colony forming units (CFUs) and erythroblasts in the liver, and erythrocytes in the blood, of f/f fetuses it is concluded that the f/f lesion is expressed at an early stage of haemopoietic development in prenatal life. The possibility that restricted haem synthesis is the primary effect of the f/f genotype and responsible for disturbances of both haemopoietic cellular proliferation and haemoglobin synthesis is examined.
D000740:Anemia / Q000151:congenital*; D000818:Animals; D001854:Bone Marrow Cells; D004912:Erythrocytes / Q000166:cytology; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D005333:Fetus / Q000166:cytology; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D008099:Liver / Q000196:embryology; D008264:Macrophages / Q000166:cytology; D051379:Mice; D011247:Pregnancy
D004921:Erythropoietin