Detergent extraction of erythrocyte ghosts. Comparison of residues after cholate and Triton X-100 treatments.
1. Human erythrocyte ghosts were extracted with individual free and conjugated bile salts and, for comparison, with Triton X-100 under conditions approximating to physiological temperature, pH and tonicity. 2. Treatment with cholate, glycocholate, taurocholate, or with Triton X-100 gave lipid-depleted residues. These could still be seen as ghost-like profiles by phase contrast microscopy. Deopxycholate brought about complete membrane dissolutiom. 3. The cholate residue gave a trilamellar image by electron microscopy and in condensed form gave a smaller membrane repeat than untreated membranes. It had a polypeptide composition representing mainly integral proteins. 4. The Triton X-100 residue had a granular profile in the electron microscope and a polypeptide composition largely representing peripheral proteins.
D001647:Bile Acids and Salts; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000032:analysis; D002458:Cell Fractionation; D002462:Cell Membrane / Q000648:ultrastructure*; D002793:Cholic Acids; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron; D011092:Polyethylene Glycols; D000644:Quaternary Ammonium Compounds
D001647:Bile Acids and Salts; D001798:Blood Proteins; D002793:Cholic Acids; D000644:Quaternary Ammonium Compounds; D011092:Polyethylene Glycols