Influence of ouabain on the fine structure of teleost retina.
In this study the effect of intraocular injection of 10 mul 10(-5) M ouabain on the retinal fine structure of the cyprinide teleost Carassius carassius is described. The final concentration of the drug in the eye-bulb was approximately 3-10(-6)M. After 8--24 h the ouabain application produces alterations in the retinal ganglion cells, that progress subsequently to necrobiosis. The outer horizontal cells of the inner nuclear layer show a severe cytoplasmic swelling that is reversible and disappears 8 days after ouabain injection. Amacrines, bipolars and receptor cells are relatively insensitive to the ouabain-induced Na+-K+-ATPase-inhibition. The differential susceptibility to ouabain in the retina possibly reflects a cell-specific pattern of Na+-K+-ATPase-activity or -concentration, whose physiological significance is discussed.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003530:Cyprinidae; D005724:Ganglia / Q000648:ultrastructure; D008870:Microtubules / Q000648:ultrastructure; D010042:Ouabain / Q000494:pharmacology*; D010786:Photoreceptor Cells / Q000648:ultrastructure; D011188:Potassium / Q000378:metabolism; D012160:Retina / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D012162:Retinal Degeneration; D012964:Sodium / Q000378:metabolism; D013569:Synapses / Q000648:ultrastructure; D013997:Time Factors
D010042:Ouabain; D000255:Adenosine Triphosphate; D012964:Sodium; D011188:Potassium