Species specific monoclonal antibodies to Bacteroides fragilis lipopolysaccharide protect mice from severe infection.
Four monoclonal antibodies which reacted in a species specific manner with Bacteroides fragilis were isolated. They recognised at least two different epitopes of B. fragilis lipopolysaccharide. The monoclonal antibodies protected non-immune mice from longlasting bacteraemia and abscess formation induced by the intraperitoneal administration of B. fragilis in combination with an infection-potentiating agent. The monoclonal antibodies were as efficient as an anti-B. fragilis hyperimmune serum. Only antibodies administered intraperitoneally or intramuscularly were protective.
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal; D016470:Bacteremia / Q000517:prevention & control; D001442:Bacteroides Infections / Q000517:prevention & control*; D001441:Bacteroides fragilis / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D016219:Immunotherapy, Adoptive; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D008070:Lipopolysaccharides