An immunohistochemical study of testicular biopsies in childhood acute lymphoblastic leukemia: reactivity of normal testicular components and leukemic infiltrates.
We performed an immunohistochemical analysis of frozen sections from testicular biopsies from 23 children with acute lymphoblastic leukemia. Eleven cases were infiltrated by leukemia. Tumor cells were immunostained by a panel of antibodies that identified CD10, CD43, CD19, CD3, CD7, and MHC class I and II. The immunoreactivity of normal testicular components was also studied. Normal testis showed no CD10 reactivity. Wide variation in the number of stromal macrophages identified by CD11c was found. Transferrin receptor (CD71) was expressed by some stromal macrophages, by seminiferous tubules, and by Leydig cells. B lymphocytes were absent from the testicular stroma but small numbers of T lymphocytes were consistently present. MHC class I and II were expressed by most stromal cells but not by seminiferous tubules.
D000293:Adolescent; D015703:Antigens, CD / Q000032:analysis*; D018941:Antigens, CD19; D019011:Antigens, CD7; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D006681:HLA-D Antigens / Q000032:analysis; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007223:Infant; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000276:immunology / Q000473:pathology*; D051917:Leukosialin; D008297:Male; D015260:Neprilysin / Q000032:analysis; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000473:pathology*; D011990:Receptors, Transferrin / Q000032:analysis; D012016:Reference Values; D012795:Sialoglycoproteins / Q000032:analysis; D013737:Testis / Q000276:immunology / Q000473:pathology*
D015703:Antigens, CD; D018941:Antigens, CD19; D019011:Antigens, CD7; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D006681:HLA-D Antigens; D015395:Histocompatibility Antigens Class I; D051917:Leukosialin; D011990:Receptors, Transferrin; C465014:SPN protein, human; D012795:Sialoglycoproteins; D015260:Neprilysin