Metabolism of alpha-aminoadipic and alpha-ketoadipic acids: studies using rat and beef liver, and human leukocytes.
The reported studies have shown that alpha-DL-amino [1-14C]adipic acid is metabolized to radioactive alpha-ketoadipic acid and then to 14CO2 in rat and beef liver homogenates. It was also demonstrated that alpha-keto [1-14C]adipic acid is decarboxylated to 14CO2. The effects of several co-factors and other variables, i.e. alpha-ketoglutarate, pyridoxal phosphate, NAD, thiamine pyrophosphate (TPP), FAD, LA, and pH have also been delimited. These reactions and the effects of the co-factors were also established in freshly drawn leukocytes. Using the methods outlined, a technique for studying the metabolism of alpha-amino-adipate and alpha-ketoadipate in the leukocytes from 10 ml of blood was developed.
D015074:2-Aminoadipic Acid / Q000378:metabolism*; D000272:Adipates / Q000378:metabolism*; D000600:Amino Acids, Dicarboxylic / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D013045:Species Specificity
D000272:Adipates; D000600:Amino Acids, Dicarboxylic; D015074:2-Aminoadipic Acid