Chromosomal sensitivity of human lymphocytes to bleomycin. Influence of antioxidant enzyme activities in whole blood and different blood fractions.
The activity of the antioxidant enzymes catalase (CAT), peroxidases (POD), and superoxide dismutases (SOD) in whole blood and different blood fractions was analyzed in 20 normal human beings and correlated with the chromosomal sensitivity of lymphocytes to bleomycin (BLM) (measured as frequency of dicentric chromosomes per BLM dose). Our results demonstrate that both the physiologic activities of the enzymes and the chromosomal sensitivity to BLM exhibit an ample and significant interindividual variability. An inverse and linear correlation between chromosomal sensitivity to BLM and the concentration of 1) CAT and POD in plasma and 2) SOD in whole blood, erythrocytes, and plasma was found. On the other hand, the chromosomal sensitivity to BLM showed a direct correlation with the concentration of SOD and POD in mononuclear leukocytes. It is suggested that a determination of antioxidant enzyme (AOE) activities in a given cell population may serve to predict the chromosomal sensitivity to BLM.
D000328:Adult; D000704:Analysis of Variance; D000975:Antioxidants; D001761:Bleomycin / Q000494:pharmacology*; D002374:Catalase / Q000097:blood; D002478:Cells, Cultured; D002875:Chromosomes / Q000187:drug effects*; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010544:Peroxidases / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood
D000975:Antioxidants; D001761:Bleomycin; D010544:Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase