Organ culture of normal and carcinogen-treated rat bladder.
A system for organ culture of rat bladder in defined medium, without serum, is described. Transitional epithelial morphology of normal bladder was well maintained in Waymouth's Medium MB 752/1, while Ham's Medium F12 caused marked epithelial hyperplasia. Hyperplastic epithelial states induced by in vivo administration of the carcinogen, N-methyl-N-nitrosourea, were well maintained in vitro during organ culture in Medium MB 752/1. The combination of hydrocortisone and insulin markedly inhibited the proliferative response of normal bladder epithelium grown in Medium F12.
D000818:Animals; D003470:Culture Media; D005260:Female; D008770:Methylnitrosourea; D009374:Neoplasms, Experimental / Q000473:pathology; D009924:Organ Culture Techniques; D011230:Precancerous Conditions / Q000473:pathology; D051381:Rats; D001743:Urinary Bladder / Q000033:anatomy & histology*; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000473:pathology*
D003470:Culture Media; D008770:Methylnitrosourea