title: Study on RNA synthesis in the retina and retinal pigment epithelium of mice by light microscopic radioautography.
abstract: With the aim of determining the distribution of the incorporation of 3H-uridine in both retina and retinal pigment epithelium (RPE), the mouse eyes at embryonic day 9.5 (E 9.5), E 12.5, E 14.5, E 16.5, E 18.5 of gestational ages, and postnatal day 1 (P 1), P 3, P 7, P 14 were analyzed by light microscopic radioautography. Small pieces of the ocular tissues were labelled with 3H-uridine in vitro and light microscopic radioautographs were prepared. The average grain numbers per cell of the respective regions of tissues were calculated. In the retina, the grain numbers increased gradually from E 9.5 to P 1 and reached the maximal value at P 1, and then decreased until P 14. However, the grain numbers were more in the vitreal portion than those in the scleral portion at E 16.5 and then became more in the scleral portion from E 18.5 to P 14. It is considered that the ganglion and bipolar cells finish the RNA synthesis earlier, while the photoreceptor cells do it later during the fetal and postnatal development. In the RPE, the grain numbers gradually increased from E 12.5 to P 7 and then decreased until P 14. Considering the same ages, the grain numbers increased in the following order, anterior, equatorial and posterior regions during embryonic stages, but decreased in the same order after birth. Therefore, it is suggested that the activity of RNA synthesis in PE cells is higher in the posterior region than in the anterior region during embryonic stages. But the activity ascends generally and becomes relatively higher in the anterior region, after birth. Comparing the retina and RPE, it was noted that the grain numbers in the RPE were more important than in the retina and that the maximal value was at P 1 in the retina, while it was at P 7 in the RPE. From these results, it can be concluded that the RNA synthesis ceases earlier in the retina than in the RPE.
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D005260:Female; D005865:Gestational Age; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D012160:Retina / Q000196:embryology / Q000254:growth & development*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
chemical_list: D014316:Tritium; D012313:RNA; D014529:Uridine