[Hyperproliferation-associated keratin 16 expression in cholesteatoma].
The position of a cell in a stratified squamous epithelium correlates with its state of differentiation as well as with the expression of certain cytokeratins. By means of both immunohistochemistry using the APAAP method and high resolution one-dimensional gel electrophoresis, specimens of cholesteatoma epithelium and normal ear skin were investigated whether they contain keratin 16 (K 16) which is known to be a marker of hyperproliferation. It could be shown that K 16 is expressed in all layers of cholesteatoma epithelium. Further to prove this fact gel electrophoresis was used showing a protein band at the localization of keratin 16. Hence, cholesteatoma can be seen as a locally hyperproliferative disease.
D002455:Cell Division / Q000502:physiology*; D002781:Cholesteatoma / Q000473:pathology*; D004427:Ear Diseases / Q000473:pathology*; D004432:Ear, Middle / Q000473:pathology*; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis*
D007633:Keratins
Hyperproliferationsassoziierte Keratin 16-Expression im Cholesteatom.