Confirmation of the human thymidine kinase locus, 17q21 leads to 17qter, by means of a man-mouse somatic cell hybrid, D98/AH-2 X LMTK-Cl-lD.
A large metacentric marker chromosome, m20, in a line of human D98/AH-2 cells was identified by Q bands as being a translocation (1;17)(p36;q21). This was confirmed by means of somatic cell hybridization between D98/AH-2 and thymidine kinase (TK) deficient mouse cells. The hybrid clones by HAT selective system retained m20, indicating the presence of TK locus on this chromosome. The results also provide evidence that TK gene is located on the distal region of the breakpoint in 17q21 but not on 17q21 leads to 17pter.
D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D002874:Chromosome Mapping; D002902:Chromosomes, Human, 16-18; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D013937:Thymidine Kinase
D013937:Thymidine Kinase