Effect of calcium depletion and subsequent repletion on parathyroids, parafollicular (C) cells and bone in the growing pig.
The ultrastructure of the chief cells of the parathroid gland and thyroid parafollicular (C) cells and the morphology of bone in calcium depletion and subsequent repletion were examined in young growing pigs. A low calcium diet resulted in osteopenia, increased removal of the cartilaginous core, osteoclasia and osteocytic osteolysis. Subsequent repletion quickly returned bone to normal. In pigs fed the low calcium diet, there was a marked depletion of secretory granules but a striking increase in the number of microtubules in chief cells. Increasing the calcium content of the diet to normal quickly returned the ultrastructural appearance of chief cells to apparent normal. In the initial response to calcium repletion, chief cells exhibited large number of lysosomes and occasionally prominent  paracrystalloid bodies. Electron microscopic examination of parafollicular (C) cells of the thyroid gland failed to reveal differences in ultrastructure between test and control pigs. These findings support the view that bone resorption following calcium deficiency may be the result of a secondary hyperparathyroidism rather than of calcium deficiency per se.
D000818:Animals; D001862:Bone Resorption / Q000209:etiology* / Q000473:pathology; D001842:Bone and Bones / Q000473:pathology; D002116:Calcitonin; D002118:Calcium / Q000172:deficiency*; D008870:Microtubules / Q000648:ultrastructure; D010280:Parathyroid Glands / Q000648:ultrastructure*; D013552:Swine; D013961:Thyroid Gland / Q000648:ultrastructure*
D002116:Calcitonin; D002118:Calcium