title: Mitotic activity and nucleic acid precursor incorporation in denervated and innervated limb stumps of axolotl larvae.
abstract: Mitotic activity and DNA and RNA precursor incorporation were compared in innervated regenerating limbs and in denervated, non-regenerating limbs on days 8 and 9 post-amputation. Innervated limbs had well-developed cone stage blastemas which showed high cellular mitotic indices and H3-thymidine labeling indices of 0.40-0.50 and H3-uridine labeling indices of 0.50-0.75. In contrast, denervated limbs showed dedifferentiated cells distally under thickened wound epithelia, but essentially no mitotic activity and no blastema formation. These dedifferentiated cells showed lower levels of H3-thymidine (0.10 index) and H3-uridine (0.50) incorporation than regenerating limbs. Labeling indices of wound epithelia are also compared.
mesh_terms: D000557:Ambystoma; D000818:Animals; D004247:DNA / Q000096:biosynthesis; D003714:Denervation; D005552:Forelimb / Q000294:innervation / Q000502:physiology; D008938:Mitosis; D012313:RNA / Q000096:biosynthesis; D012038:Regeneration; D013936:Thymidine / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D012313:RNA; D004247:DNA; D013936:Thymidine; D014529:Uridine