Gas chromatography of bile acids as their hexafluoroisopropyl ester-trifluoroacetyl derivatives.
Bile acids, such as cholic, chenodeoxycholic, deoxycholic, lithocholic and ursodeoxycholic acids, were allowed to react with hexafluoroisopropanol and tri-fluoracetic anhydride at 37 for 30 min. The resulting derivatives were gas chromatographed on QF-1, with flame ionization detection, and were identified by gas chromatography-mass spectrometry. Separation was good. By using this method, these acids were detected in samples of human duodenal fluid; the ratios of each were 24.4, 41.5, 24.9, 2.3 and 6.9%, respectively.
D001647:Bile Acids and Salts / Q000032:analysis*; D002849:Chromatography, Gas; D006801:Humans; D007202:Indicators and Reagents; D007419:Intestinal Secretions / Q000032:analysis; D013058:Mass Spectrometry; D008722:Methods; D020005:Propanols; D014269:Trifluoroacetic Acid; D006845:Hydrocarbons, Fluorinated
D001647:Bile Acids and Salts; D007202:Indicators and Reagents; D020005:Propanols; D014269:Trifluoroacetic Acid; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated