title: Age-related changes in galanin- and calretinin-immunoreactive nerves of guinea-pig gallbladder.
abstract: Age-related changes in the expression of galanin- and calretinin-like immunoreactivity in the ganglionated plexus of 2-4 day, 6 month and 2 year old guinea-pig gallbladder were investigated. The ganglionated plexus was studied using immunohistochemical labelling techniques on whole mount stretch preparations of the gallbladders. Galanin- and calretinin-like immunoreactivity did not differ significantly between age groups of the 2-4 day and 6 month olds except for some reduction of calretinin-immunofluorescent nerves in the 6 month old preparations. However, in the ganglionated plexus of the 2 year old guinea-pig gallbladders, neither galanin- nor calretinin-like immunoreactivity could be detected. The implication of these findings, in relation to the physiological activity of the gallbladder is discussed.
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D064032:Calbindin 2; D019004:Galanin; D005704:Gallbladder / Q000294:innervation* / Q000378:metabolism; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D010455:Peptides / Q000276:immunology / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000276:immunology / Q000378:metabolism*
chemical_list: D064032:Calbindin 2; D010455:Peptides; D064030:S100 Calcium Binding Protein G; D019004:Galanin