title: Comparative studies on binding of vitronectin and fibronectin to groups A and C streptococci.
abstract: Binding of 125I-labelled fibronectin and vitronectin to streptococci of group A (S. pyogenes), group B (S. agalactiae) and group C (S. dysgalactiae and S. zooepidemicus) isolated from various human infections and bovine mastitis, and S. uberis bovine isolates, was studied. Binding of vitronectin and fibronectin was common among both human groups A and C, and bovine group C streptococci. S. agalactiae strains of human and bovine origin as well as S. uberis bovine isolates bound low levels of both proteins. The binding of radiolabelled fibronectin and vitronectin to selected groups A and C streptococcal strains was specific, time-dependent and occurred with both live and heat-killed (80 degrees C for 15 min) cells. Binding declined rapidly after treatment of cells with trypsin or proteinase K, while pepsin digestion at pH 5.5 affected vitronectin but not fibronectin binding.
mesh_terms: D003470:Culture Media / Q000737:chemistry; D005353:Fibronectins / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D010434:Pepsin A / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D013045:Species Specificity; D013291:Streptococcus / Q000145:classification / Q000187:drug effects / Q000378:metabolism; D013292:Streptococcus agalactiae / Q000187:drug effects / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology; D019096:Vitronectin
chemical_list: D003470:Culture Media; D005353:Fibronectins; D006023:Glycoproteins; D019096:Vitronectin; D014357:Trypsin; D010434:Pepsin A