title: Adsorption of tryptophan metabolites from physiological fluids on XAD-2 and determination by single ion monitoring.
abstract: Endogenous tryptamine, 5-hydroxytryptamine, indoleacetic acid, 5-hydroxyindoleacetic and tryptophan have been recovered from urine and cerebro-spinal fluid by adsorption on XAD-2 resin (0.3 g). After adsorption of the sample on the resin, desorption with methanol provides a single fraction that contains all of these metabolites. The mass spectra of their pentafluoropropionyl derivatives show prominent ions at m/e 276 and 438 which are characteristic of indoles and 5-hydroxyindoles, respectively, a feature that allows the concurrent determination of all the components of each group by functional group analysis. A method has been developed to carry out single ion monitoring with the peak matching system of an Hitachi RMU-6H mass spectrometer. Identifications are based on the respective Kovats Indices and single ion monitoring of two characteristic ions per compound: tryptophan (m/e 276 and 347); tryptamine (m/e 276 and 289); indoleacetic acid (m/e 276 and 335); 5-hydroxytryptamine (m/e 438 and 451); 5-hydroxyindoleacetic acid (m/e 438 and 497). The method described illustrates the feasibility of assaying biogenic indoleamines and acidic metabolites, as well as their precursor amino acid on a single fraction in contrast to other standard fractionation methods. This is possible even if the mass spectrometer is not equipped with an alternating voltage accelerator provided that it has a peak matcher, although the lack of an alternating voltage accelerator requires two separate injections of the same sample, for quantification and identification; one for the indole profile and another for the 5-hydroxyindole profile. Both profiles can be verified by individual monitoring of the other confirmatory ions. With this method the use of a multiple ion detector would allow a simultaneous determination of all of these metabolites in one gas chromatograph mass spectrometer run.
mesh_terms: D000327:Adsorption; D002849:Chromatography, Gas; D007210:Indoleacetic Acids / Q000032:analysis; D013058:Mass Spectrometry; D012117:Resins, Synthetic; D012997:Solvents; D014363:Tryptamines / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000652:urine; D014364:Tryptophan / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000652:urine
chemical_list: D007210:Indoleacetic Acids; D012117:Resins, Synthetic; D012997:Solvents; D014363:Tryptamines; D014364:Tryptophan