In vivo and in vitro fertilization of hamster, rat and mouse eggs after treatment with anti-hamster ovary antiserum.
Rabbit antiserum against hamster ovary was examined on agargel diffusion plates against several hamster tissues, and also against rat and mouse ovarian extracts. Unabsorbed anti-hamster ovary antiserum showed eight to nine precipitin bands for hamster ovary and four to eight bands for other tissue extracts, but no bands against sperm antigens. Anti-hamster ovary antiserum also showed three to four bands for rat and one to two bands for mouse ovarian extracts. The present experiments confirmed previous reports for the hamster and mouse that treatment of eggs with anti-ovary antiserum blocked in vitro fertilization and that the extent of the inhibition was related to the formation of a precipitate on the zone pellucida. A single injection of anti-hamster ovary antiserum inhibited fertilization in mice but not in rats. In vitro fertilization of mouse eggs was also inhibited in the presence of such antiserum.
D000818:Animals; D006224:Cricetinae; D005260:Female; D005306:Fertilization; D007106:Immune Sera; D066298:In Vitro Techniques; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D008297:Male; D051379:Mice; D010053:Ovary / Q000276:immunology*; D010063:Ovum / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D015044:Zona Pellucida / Q000276:immunology
D007106:Immune Sera