Semi-automated competitive protein binding analysis of serum thyroxine on reusable Sephadex columns and its advantages over radioimmunoassay.
Competitive protein-binding analysis of serum thyroxine on small, reusable, Sephadex columns has been further studied and improved. The improved, semi-automated procedure results in reduced working time and costs. It has also been established that triiodothyronine crossreacts only 1/6 to 1/9 as well as thyroxine, and can be ignored because it represents only about 1/80 of the total serum iodothyronine content. The economic and methodological advantages of the improved method over radioammunoassay and other displacement assays are discussed.
D000837:Anion Exchange Resins; D001667:Binding, Competitive; D002852:Chromatography, Ion Exchange / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods; D011869:Radioligand Assay; D013974:Thyroxine / Q000097:blood*
D000837:Anion Exchange Resins; D013974:Thyroxine