Structure of the bovine endothelin-B receptor gene and its tissue-specific expression revealed by northern analysis.
We have isolated and characterized the gene encoding the bovine endothelin (ET) receptor ETB with seven putative membrane-spanning domains. The gene exists as a single copy of the bovine genome and extends over 36 kilobases. The coding region is divided into seven exons separated by six introns; the first exon encodes the first and second transmembrane domains and each of the following transmembrane domains is encoded by a separate exon. The 5' region of the transcript was examined by the ribonuclease protection analysis; multiple start sites were deduced with no apparent "TATA" box in the expected upstream region. Northern blot analysis demonstrated a widespread but tissue-specific distribution of the ETB receptor in bovine tissues.
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002417:Cattle; D005091:Exons; D015870:Gene Expression; D007438:Introns; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D017466:Receptors, Endothelin / Q000737:chemistry / Q000235:genetics*
D017466:Receptors, Endothelin