The uptake of delta9-tetrahydrocannabinol in choroid plexus and brain cortex in vitro and in vivo.
[3H]delta9 Tetrahydrocannabinol (delta9-THC) was actively transported by the choroid plexus and cerebral cortical slices of the rabbit when incubated as a BSA-microsuspension in artificial rabbit CSF. The transport system for delta9-THC in choroid plexus had a V max of 174 nmoles/mg tissue/h, approximately 9-fold greater than that observed for cortical slices. In vivo experiments demonstrated a preferential distribution of delta9-THC in choroid plexus at 1 h after intravenous injection. These results indicate that delta9-THC is actively accumulated by choroidal epithelium and may also be transported across the epithelial stroma into the capillary circulation. This suggests that the choroid plexus participates in the regulation of delta9-THC concentration in CSF and indirectly in brain by means of the "sink" function of the CSF.
D000818:Animals; D001693:Biological Transport, Active; D002188:Cannabis / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D002831:Choroid Plexus / Q000378:metabolism*; D013759:Dronabinol / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits
D013759:Dronabinol