title: Lipophosphonoglycan of the plasma membrane of A canthamoeba castellanii. Inositol and phytosphingosine content and general structural features.
abstract: Lipophosphonoglycan, a major component of the plasma membrane of Acanthamoeba castellanii, has now been shown to contain 8% inositol and 13% C25- and C24-phytosphingosines in addition to the previously identified content of neutral sugars (26%), amino sugars (3%), aminophosphonates (10%), acidhydrolyzable phosphate (3%), and long chain fatty acids (14%). The fatty acids and phytosphingosines are in ceramide groups. Lipophosphonoglycan can be separated by dodecyl sulfate-polyacrylamide electrophoresis into two major components that are similar in composition except for different oligosaccharide groups. A tentative structural model incorporating these features is proposed in which each of the two components of lipophosphonoglycan is conceived as an oligomeric inositol-containing glycosphingolipid.
mesh_terms: D000656:Amoeba / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis*; D002518:Ceramides / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006028:Glycosphingolipids / Q000032:analysis*; D006601:Hexoses / Q000032:analysis; D007294:Inositol / Q000032:analysis*; D063065:Organophosphonates / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D013110:Sphingosine / Q000032:analysis / Q000031:analogs & derivatives
chemical_list: D002518:Ceramides; D005227:Fatty Acids; D006028:Glycosphingolipids; D006601:Hexoses; D063065:Organophosphonates; D010710:Phosphates; C008290:lipophosphonoglycan; D007294:Inositol; C012491:phytosphingosine; D013110:Sphingosine