Physical studies on glycoproteins from ascitic fluid of Ehrlich ascites tumor.
Three glycoproteins, designated as F, M and S glycoproteins were identified in the HCIO4-soluble fraction of ascitic fluid of Ehrlich ascites tumor by 8% polyacrylamide disc gel electrophoresis. They were separated and purified as described previously (Reznick, A.Z. and Winzler, R.J. (1973) Fed. Proc. 32, 368 and Reznick, A.Z., Allen, H.J. and Winzler, R.J. (1973) Anal. Biochem. 52, 395-401) and subjected to physical characterization. Several physical properties such as molecular weights, sedimentation and diffusion coefficients, partial specific volumes, Stoke's radii and frictional ratios were determined. The physical parameters of F and S glycoproteins resemble data that have been reported for orosomucoid and haptoglobin-like glycoproteins, respectively. Properties of M glycoprotein could not be associated with a known glycoprotein.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D002850:Chromatography, Gel; D006023:Glycoproteins; D051379:Mice; D008970:Molecular Weight; D009997:Osmotic Pressure; D011487:Protein Conformation; D014461:Ultracentrifugation
D006023:Glycoproteins