Purification of a lysinonorleucine cross-linked peptide fraction from porcine aorta elastin.
Highly purified elastin from porcine aorta was submitted to elastase digestion. The enzymic products were subjected to gel filtration on Sephadex G 25. The excluded fraction was then submitted to thermolysin digestion and gel filtration. The excluded fraction was submitted to partial acid hydrolysis and gel filtration. Several sub-fractions were obtained. The F3 subfraction containing cross-linking agents (desmosines and lysinonorleucine) was finally subjected to ion exchange chromatography. A highly enriched peptide fraction containing lysinomorleucine was obtained and then purified by preparative electrophoresis. The ratio of enrichment passed from 2 residues of lysinonorleucine (expressed as lysine) from starting material (elastin) to 178 out of 1,000 residues in the final step. In this peptide fraction, if we express in molar ratio and consider the amount of lysinonorleucine is one residue, the following amino-acid composition is Pro:3, Gly:1, Ala:2, LNL:1, Lys:2. No traces of desmosines are detected. The role of lysinonorleucine in bridging functions in elastin is discussed.
D000596:Amino Acids / Q000032:analysis; D000614:Aminocaproates / Q000032:analysis*; D000818:Animals; D001011:Aorta / Q000032:analysis*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004549:Elastin / Q000032:analysis*; D008239:Lysine / Q000032:analysis*; D009646:Norleucine / Q000032:analysis*; D010196:Pancreatic Elastase; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification*; D013552:Swine; D013820:Thermolysin
D000596:Amino Acids; D000614:Aminocaproates; D010446:Peptide Fragments; D009646:Norleucine; D004549:Elastin; D010196:Pancreatic Elastase; D013820:Thermolysin; D008239:Lysine