Infection of macaque monkeys with a chimeric human and simian immunodeficiency virus.
Two macaque monkeys were inoculated with a chimeric human and simian immunodeficiency virus carrying the tat, rev, vpu and env genes of human immunodeficiency virus type 1. Infectious virus was recovered from one of the monkeys at 2 and 6 weeks post-infection. The hybrid nature of the isolated viruses was verified by Southern and Western blotting analyses. Both of the monkeys infected with the chimera elicited a humoral antibody response against the virus.
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002874:Chromosome Mapping; D005814:Genes, Viral / Q000235:genetics*; D015752:Genes, env / Q000235:genetics; D015757:Genes, rev / Q000235:genetics; D015756:Genes, tat / Q000235:genetics; D016338:Genes, vpu / Q000235:genetics; D015658:HIV Infections / Q000235:genetics* / Q000662:veterinary*; D008251:Macaca; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D012190:Retroviridae / Q000254:growth & development; D016097:Simian Acquired Immunodeficiency Syndrome / Q000235:genetics*
D000914:Antibodies, Viral; D012367:RNA, Viral; C039919:RNA, recombinant; D012313:RNA