Gas-chromatographic determination of an antifibrinolytic drug, epsilon-aminocaproic acid.
We describe a modified method for assay of epsilon-aminocaproic acid. Serum or cerebrospinal fluid is deproteinized, followed by cation-exchange column-chromatography, and N-trifluoroacetyl-n-butyl derivatives of amino acids are formed and separated by gas chromatography. Tranexamic acid, a nonprotein amino acid, was used as an internal standard. The assay is sensitive and precise, and results correlate adequately with those obtained with an automated amino acid analyzer (ion-exchange chromatography).
D000596:Amino Acids / Q000032:analysis; D000614:Aminocaproates / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D001322:Autoanalysis; D002849:Chromatography, Gas / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D008832:Microchemistry
D000596:Amino Acids; D000614:Aminocaproates