Injury of mouse lymphocytes caused by exogenous methyl linoleate hydroperoxides in vitro.
The incubation with methyl linoleate hydroperoxides (MLHPO), a model of lipid peroxides, depressed DNA, RNA and protein syntheses of mouse thymic lymphocytes and increased the amount of thiobarbituric acid-reactive substances in lymphocytes. These phenomena were also found in the splenic lymphoblasts in the DNA synthetic phase (S-phase) obtained by mitogen. Prior culturing with all-rac-alpha-tocopherol increased DNA synthesis in splenic lymphoblasts. Electron microscopically, cytoplasmic micro-organelles of splenic lymphoblasts in the S- and G2-phases were markedly destroyed as compared with nuclei. No discernible changes were observed in not-blastotransformed lymphocytes under these experimental conditions. These findings indicate that thymic lymphocytes and splenic lymphoblasts are affected by exogenous lipid peroxides, and cytoplasmic micro-organelles of splenic lymphoblasts might be markedly damaged by exogenous lipid peroxides as compared to their nuclei.
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D008054:Lipid Peroxides / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis / Q000187:drug effects; D013154:Spleen / Q000166:cytology; D013858:Thiobarbiturates / Q000737:chemistry; D013950:Thymus Gland / Q000166:cytology; D014810:Vitamin E / Q000737:chemistry
D008054:Lipid Peroxides; D013858:Thiobarbiturates; C008464:methyl linoleate hydroperoxide; D014810:Vitamin E; D012313:RNA; D004247:DNA; C029684:thiobarbituric acid