Purification and biochemical characterization of squirrel monkey retrovirus-H produced in a human lymphoblastoid cell line.
We found a type D retrovirus in a human lymphoblastoid cell line of B-cell lineage. Molecular cloning and nucleotide sequencing of the provirus genome revealed that this virus was closely related to squirrel monkey retrovirus (SMRV), and we designated this virus as SMRV-H. To investigate the relationship between these retroviruses, SMRV-H was purified from the virus-producing cells, and its biochemical properties were characterized. The cell-adhesive virus particles were successfully separated from the cell by a brief trypsin treatment and purified by velocity sedimentation. The purification of the virus was confirmed by electron microscopy. Major gag protein of the virus is phosphorylated, and has a molecular weight of 34 kDa. The virion-associated reverse transcriptase prefers Mg2+ to Mn2+. These properties of SMRV-H were almost the same as those of SMRV.
D000818:Animals; D001402:B-Lymphocytes; D030121:Betaretrovirus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002413:Cations, Divalent; D002460:Cell Line; D003001:Cloning, Molecular; D015683:Gene Products, gag / Q000032:analysis; D016679:Genome, Viral; D006801:Humans; D008970:Molecular Weight; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012453:Saimiri / Q000382:microbiology*; D013698:Templates, Genetic; D015678:Viral Structural Proteins / Q000302:isolation & purification; D014771:Virion / Q000235:genetics / Q000302:isolation & purification*
D002413:Cations, Divalent; D015683:Gene Products, gag; D015678:Viral Structural Proteins; D012194:RNA-Directed DNA Polymerase