Morphometric and biochemical studies in trigeminal nerve of rat after trichloroethylene or dichloroacetylene oral administration.
Trigeminal nerve impairment is one of the main features of the clinical spectrum observed after trichloroethylene (Tce) exposure. A morphometric study of teased fibres, an analysis of the fatty acid composition of total lipids of the trigeminal trunk, measurements of myelin basic protein (MBP) and of 2'-3'-cyclic nucleotide 3' phosphohydrolase (CNP) activity were used to characterize this lesion. Furthermore, the effects of Tce and of dichloroacetylene (Dca), a breakdown product supposed to be the main cause of the trigeminal neuropathy, were compared under the same experimental conditions. Results showed a significant decrease of the internode length and of the mean fibre diameter in the Dca group whereas these values, although also diminished, were not significantly affected in the Tce group. In the trigeminal nerve, this was associated with a significant (46%) decrease in sigma. (n-6) fatty acid and a 22% decrease in sigma. (n-3) fatty acid contents in the Tce group whereas these decreases were respectively 32% in sigma. (n-6) fatty acid and 27% (significant) in sigma. (n-3) fatty acid contents in the Dca group. In this group, a 64% increase in sigma. Fatty aldehydes was also observed. Finally, Tce or Dca had only a slight effect on the overall profile of fatty acid content in the brain and no significant variation was observed in MBP level and CNP activity. Both compounds can thus induce trigeminal nerve alterations, but with a higher intensity for Dca.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism*; D000114:Acetylene / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D000284:Administration, Oral; D000818:Animals; D001921:Brain / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification; D004676:Myelin Basic Protein / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014241:Trichloroethylene / Q000008:administration & dosage / Q000633:toxicity*; D014276:Trigeminal Nerve / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D005227:Fatty Acids; D008055:Lipids; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D014241:Trichloroethylene; C002978:dichloroacetylene; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; C039809:2',3'-cyclic nucleotide 2'-phosphodiesterase; D000114:Acetylene