Presence and subcellular localization of two distinct mitogenic fractions in the cells of Nocardia rubra and Nocardia opaca: preparation of soluble mitogenic peptidoglycan fractions.
Fractionation of cells of Nocardia rubra and Nocardia opaca led to the separation of the cell wall and a "cytoplasmic" fraction. Both fractions were mitogenic for the splenocytes of AKR and nude mice and of rabbits. The peptidoglycan was the active part of the cell wall fraction. The products solubilized by the action of Streptomyces albus G peptidases on the peptidoglycan of N. rubra were mitogenic, but the products solubilized by lysoyme were not. Tentative structures are proposed for these fractions. The most active part of the cytoplasmic fractions could be sedimented by centrifugation and seemed to be related to the cytoplasmic membrane.
D000818:Animals; D002458:Cell Fractionation; D002473:Cell Wall / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D008213:Lymphocyte Activation; D051379:Mice; D008819:Mice, Nude; D008934:Mitogens / Q000032:analysis / Q000302:isolation & purification*; D009615:Nocardia / Q000276:immunology*; D010457:Peptidoglycan / Q000302:isolation & purification*; D011817:Rabbits; D012995:Solubility; D013347:Subcellular Fractions / Q000032:analysis
D008934:Mitogens; D010457:Peptidoglycan