Direct application to dairy foods of a Listeria-specific oligonucleotide probe to 16S rRNA.
A potentially Listeria-specific 28 base oligonucleotide probe was designed from 16S rRNA sequence data. Using either 32P or non-radioactive (alkaline phosphatase) labels, the probe was shown to be highly specific as it hybridised to RNA extracted from all of the species of Listeria but not to any of the other bacteria tested. Both probe methods were highly sensitive and ca 10(2) cfu/ml Listeria could be detected in pure cultures. A rapid procedure for extracting RNA from milk, Camembert and cottage cheese was developed. This allowed the direct application of the probe to these foods and gave a rapid and specific method of detecting > 10(2) cfu/g or ml Listeria in these foods.
D001483:Base Sequence; D003124:Colorimetry / Q000379:methods; D003611:Dairy Products / Q000382:microbiology*; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification
D015345:Oligonucleotide Probes; D012329:RNA, Bacterial