[Comparison of the nature of insulin secretion, determined by radioimmune and biological methods, normally and in diabetes mellitus].
The character of insulin secretion curves was studied under normal conditions and in diabetic patients during glucose tolerance test. Patients with diabetes mellitus were divided into the following groups: donors, patients with reduced glucose tolerance and with manifest diabetes. Insulin was determined in paralleled by 3 methods (biological ones and radioimmune one). An increase in immunoreactive insulin was seen in the group of patients with reduced glucose tolerance during the whole course of the test with a peak at the 30th minute and a simultaneous reduction and a sharp fall at the 60th minute of free insulin level. Apparently biologically less active forms of insulin and proinsulin are secreted which possess immunological properties and react well with antibodies to insulin.
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003920:Diabetes Mellitus / Q000503:physiopathology*; D005215:Fasting; D005609:Free Radicals; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D000078790:Insulin Secretion; D008297:Male; D011485:Protein Binding; D011863:Radioimmunoassay; D051381:Rats; D013997:Time Factors
D001786:Blood Glucose; D005609:Free Radicals; D007328:Insulin
Sravnenie kharaktera sekretsii insulina, opredeliaemogo radioimmunnym i biologikeskimi metodami, v norme i pri sakharnom diabete