Binding of secretory component to dimers of immunoglobulin A in vitro. Mechanism of the covalent bond formation.
A complex between secretory component and an immunoglobulin A (IgA) myeloma dimer has been studied in vitro as a model to elucidate the mechanism of the formation of disulfide bonds during assembly in vivo of secretory immunoglobin A. A small amount of free thiol groups, totally about 0.4 groups per mole of protein, were shown to be present on both the heavy and light chains of the IgA dimer, but not on its J-chain, while no such groups could be demonstrated on free secretory component. The SH-groups on IgA most likely exist as a result of incomplete oxidation of some intra-or interchain disulfide bonds of the molecule, analogous to what has been suggested for IgG. Several types of evidence indicated that the disulfide bonds between secretory component and IgA are formed after the noncovalent association of the two proteins by a sulfhydryl group-disulfide bond exchange reaction, in which the small amount of free sulfhydryl groups on the IgA dimer initiate the reaction by reducing a reactive disulfide bond on secretory component. This exchange reaction, which thus proceeds by the mechanism of so-called disulfide interchange reactions, requires certain conformational features of one or both of the proteins and leads to the formation of presumably two new interchain disulfide bonds between secretory component and IgA. The reaction does not progress to completion, however, but ends in an equilibrium so that a small proportion of the secretory component molecules always are unattached by disulfide bonds.
D001665:Binding Sites; D001798:Blood Proteins; D002729:Chloromercuribenzoates; D004220:Disulfides / Q000032:analysis; D006146:Guanidines; D006801:Humans; D007070:Immunoglobulin A; D007460:Iodoacetamide; D007467:Iodoproteins; D046911:Macromolecular Substances; D009194:Myeloma Proteins; D011485:Protein Binding; D011487:Protein Conformation
D001798:Blood Proteins; D002729:Chloromercuribenzoates; D004220:Disulfides; D006146:Guanidines; D007070:Immunoglobulin A; D007467:Iodoproteins; D046911:Macromolecular Substances; D009194:Myeloma Proteins; D007460:Iodoacetamide