Role of tyrosine residues in cytoplasmic aspartate aminotransferase from beef kidney.
Cytoplasmic aspartate aminotransferase from beef kidney loses 25% of its activity on nitration with tetranitromethane while the apoenzyme about 95%. In the holoenzyme 0.5 tyrosine residue and 1.0 tyrosine residue in the apoenzyme are nitrated per enzyme protomer. In addition 1 cysteine residue per protomer is oxidized in both. The presence of substrates, alpha-ketoglutarate and glutamate, both at ten times their Km values, does not change these results. Mercaptoethanol does not affect the residual activity of either the nitrated holo or apoenzyme. Dithionite abolishes the activity of the nitrated holoenzyme by reducing tha coenzyme moiety. It has no effect on the native holoenzyme or on either the native or nitroapoenzyme.
D000818:Animals; D001051:Apoenzymes; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D001665:Binding Sites; D002417:Cattle; D004227:Dithionite / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008623:Mercaptoethanol / Q000494:pharmacology; D008626:Mercuribenzoates / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology*; D008970:Molecular Weight; D011485:Protein Binding; D011487:Protein Conformation; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013774:Tetranitromethane / Q000494:pharmacology; D014443:Tyrosine / Q000032:analysis
D001051:Apoenzymes; D008626:Mercuribenzoates; D004227:Dithionite; D014443:Tyrosine; D008623:Mercaptoethanol; D001219:Aspartate Aminotransferases; D013774:Tetranitromethane