The effect of 5-bromodeoxyuridine on cell division and differentiation of preimplantation mouse embryos.
Mouse embryos exposed to concentrations of 5-bromodeoxyuridine (BUdR) ranging from 0-01 to 1-0 mug/ml in vitro for two days from the 8-cell stage exhibit a concentration-dependent decrease in the frequency of normal blastocysts and decrease in average cell number per embryo. A 20-h exposure was adequate to achieve the full BUdR response. Both effects were eliminated in the presence of excess thymidine. Autoradiographs demonstrated that BUdR[3H] was incorporated into DNA during the first and second day of culture. Thus, BUdR appears to act through incorporation into DNA; and, in this system, cell division is at least as sensitive to BUdR as is differentiation.
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003841:Deoxycytidine / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000187:drug effects*; D051379:Mice; D013936:Thymidine / Q000494:pharmacology
D003841:Deoxycytidine; D001973:Bromodeoxyuridine; D013936:Thymidine