A rapid technique for visualizing cerebral arteries in rat.
Intracardiac perfusion with cresyl violet acetate dissolved in 0.9% isotonic saline and dimethyl sulfoxide (DMSO) allows one to visualize the arterial blood supply to the central hemispheres. This technique is especially useful in demonstrating the ramifications of and areas supplied by the middle cerebral artery. Many individual differences in the course traversed by the artery and the distal areas supplied were observed. This technique could prove to be a useful adjunct to neurohistological techniques in studies investigating rodent models of stroke.
D000818:Animals; D048588:Benzoxazines; D002536:Cerebral Arteries / Q000473:pathology*; D002540:Cerebral Cortex / Q000098:blood supply*; D002544:Cerebral Infarction / Q000473:pathology*; D004121:Dimethyl Sulfoxide; D004292:Dominance, Cerebral / Q000502:physiology; D006652:Histological Techniques; D010078:Oxazines; D010477:Perfusion; D051381:Rats; D013194:Staining and Labeling
D048588:Benzoxazines; D010078:Oxazines; C028911:cresyl violet; D004121:Dimethyl Sulfoxide