[Detection of clonal immunoglobulin rearrangements in paraffin embedded tissues by PCR].
A polymerase chain reaction based method was established using immunoglobulin VH and VK gene rearrangements as markers to detect clonal B cell populations in paraffin embedded sections. Family specific VH and VK primers are used in separate reactions together with the corresponding J primers to amplify rearranged VH and VK genes from genomic DNA. This allows to distinguish clonal from polyclonal B cell populations in most of the cases. The method may be helpful in routine diagnosis of B cell NHL and some morphological and immunohistochemical difficult cases.
D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006652:Histological Techniques; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007938:Leukemia / Q000235:genetics / Q000276:immunology*; D008223:Lymphoma / Q000175:diagnosis / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010232:Paraffin; D016133:Polymerase Chain Reaction / Q000379:methods
D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D009838:Oligodeoxyribonucleotides; D010232:Paraffin
Nachweis monoklonaler Immunglobulingen-Umlagerungen in Paraffinschnitten mittels PCR.