Immunoreactive gastrin in differential and density gradient fractions of rat gastric mucosa.
Immunoreactive gastrin was measured in subcellular fractions of rat gastric mucosa. The sedimentational properties of subcellular gastrin-containing structures were distinct from those of mitochondria. After centrifugation in sucrose density gradients using a zonal rotor, the peak of immunoreactive gastrin was found in 1-17--1-18 g cm(-3) density sucrose (1-35 M; 39-5%, W/W). A thermolabile component with 125I-labelled gastrin-binding activity present in gastric mucosal homogenates and fractions was not associated with the gastrin storage vesicles sedimenting in density gradients.
D000818:Animals; D005753:Gastric Mucosa / Q000032:analysis* / Q000201:enzymology; D005755:Gastrins / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D013347:Subcellular Fractions / Q000032:analysis; D013385:Succinate Dehydrogenase / Q000032:analysis
D005755:Gastrins; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase