title: Chitinous components of the cell wall of Fusarium oxysporum.
abstract: The cell wall of Fusarium oxysporum f. sp. lycopersici was digested with chitinase to analyze the structure of its chitinous components. In spite of a similar acetylation degree of the cell wall components to that of 25-35% acetylated chitosan, only N-acetylglucosamine disaccharide [(GlcNAc)2] was obtained from chitinase hydrolyzate of the fungal cell wall by CM-Sephadex C-25 column chromatography, while (GlcNAc)2 and several types of deacetylated chitooligosaccharides were separated from that of 25-35% acetylated chitosan. The results indicate that N-acetylglucosamine residues in the polysaccharide chains of the fungal cell wall are most likely condensed into some region, while acetylated residues are more scattered in 25-35% acetylated chitosan.
mesh_terms: D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D002686:Chitin / Q000031:analogs & derivatives / Q000032:analysis*; D002688:Chitinases / Q000378:metabolism; D048271:Chitosan; D002845:Chromatography / Q000379:methods; D003911:Dextrans; D005670:Fusarium / Q000737:chemistry* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D013055:Spectrophotometry, Infrared
chemical_list: D002241:Carbohydrates; D003911:Dextrans; D002686:Chitin; D048271:Chitosan; C025614:sephadex; D002688:Chitinases