Temporary inhibition of antibody-dependent, cell-mediated cytotoxicity by pretreatment of human attacking cells with ammonium chloride.
Treatment of lymphoid cell preparations with ammonium chloride buffers to remove contaminating erythroid cells markedly reduced the ability of these cells to function as attacking cells in antibody dependent cell mediated cytotoxicity tests (ADCC). The lymphoid cells, however, retained their ability to adhere to antibody-coated target cells. Recovery of function 20 hr after exposure to ammonium chloride suggests a metabolic rather than receptor effect of these salts on the ADCC function of lymphocytes.
D000643:Ammonium Chloride / Q000494:pharmacology*; D000918:Antibody Specificity; D002021:Buffers; D006801:Humans; D007104:Immune Adherence Reaction; D007111:Immunity, Cellular; D008214:Lymphocytes / Q000378:metabolism; D013997:Time Factors
D002021:Buffers; D000643:Ammonium Chloride