title: [Energizing plasmalemma of yeasts is necessary for activation of its H+-ATPase by glucose].
abstract: ATPase of yeast plasmalemma is known to be activated during incubation of cells or protoplasts with glucose. It has been shown that the level of ATPase activation is sharply decreased after pretreatment of cells or protoplasts with mercaptoethanol, dinitrophenol, gramicidin D, nigericin, or monensin. It is suggested that deenergization of yeast plasmalemma by monensin, nigericin, and mercaptoethanol as uncoupler plays a crucial role in the prevention of in vivo activation of plasma membrane ATPase by glucose. It is concluded that energization of yeast plasmalemma is necessary for activation of ATPase by glucose.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004140:Dinitrophenols / Q000494:pharmacology; D004789:Enzyme Activation; D005947:Glucose / Q000494:pharmacology*; D006096:Gramicidin / Q000494:pharmacology; D007476:Ionophores; D008623:Mercaptoethanol; D008985:Monensin / Q000494:pharmacology; D009550:Nigericin / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012440:Saccharomyces / Q000201:enzymology*
chemical_list: D004140:Dinitrophenols; D007476:Ionophores; D006096:Gramicidin; D008623:Mercaptoethanol; D000255:Adenosine Triphosphate; D008985:Monensin; D006180:Proton-Translocating ATPases; D005947:Glucose; D009550:Nigericin
vernacular_title: Energizatsiia plazmalemmy drozhzheĭ neobkhodima dlia aktivatsii ee H+-ATPazy gliukozoĭ.