Biochemical evidence associating membrane-encapsidated particles with reverse transcription in human gastric cancer.
We provide biochemical evidence demonstrating that membrane encapsidated structures present in terminal gastric patients share similar features with retrovirus-like particles. Purified particles reveal few polypeptides, one of them glycosylated. A ribonucleoprotein core-like component is recovered which retains the DNA-polymerizing activity and contains at least one RNA component which can be efficiently translated in vitro.
D000230:Adenocarcinoma / Q000382:microbiology* / Q000648:ultrastructure; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis; D008566:Membranes / Q000382:microbiology / Q000648:ultrastructure; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012194:RNA-Directed DNA Polymerase / Q000032:analysis*; D012190:Retroviridae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D012261:Ribonucleoproteins / Q000032:analysis; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000648:ultrastructure
D008565:Membrane Proteins; D012261:Ribonucleoproteins; D012194:RNA-Directed DNA Polymerase