Inhibition of RNA polymerase by captan at both DNA and substrate binding sites.
RNA synthesis carried out in vitro by Escherichia coli RNA polymerase was inhibited irreversibly by captan when T7 DNA was used as template. An earlier report and this one show that captan blocks the DNA binding site on the enzyme. Herein, it is also revealed that captan acts at the nucleoside triphosphate (NTP) binding site, and kinetic relationships of the action of captan at the two sites are detailed. The inhibition by captan via the DNA binding site of the enzyme was confirmed by kinetic studies and it was further shown that [14C]captan bound to the beta' subunit of RNA polymerase. This subunit contains the DNA binding site. Competitive-like inhibition by captan versus UTP led to the conclusion that captan also blocked the NTP binding site. In support of this conclusion, [14C]captan was observed to bind to the beta subunit which contains the NTP binding site. Whereas, preincubation of RNA polymerase with both DNA and NTPs prevented captan inhibition, preincubation with either DNA or NTPs alone was insufficient to protect the enzyme from the action of captan. Furthermore, the interaction of [14C]captan with the beta and beta' subunits was not prevented by a similar preincubation. Captan also bound, to a lesser extent, to the alpha and sigma subunits. Therefore, captan binding appears to involve interaction with RNA polymerase at sites in addition to those for DNA and NTP; however, this action does not inhibit the polymerase activity.
D001426:Bacterial Proteins / Q000037:antagonists & inhibitors; D017123:Bacteriophage T7 / Q000235:genetics; D001665:Binding Sites / Q000187:drug effects; D002215:Captan / Q000378:metabolism / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D046911:Macromolecular Substances; D012313:RNA / Q000096:biosynthesis; D013698:Templates, Genetic; D014544:Uridine Triphosphate / Q000494:pharmacology
D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D004279:DNA, Viral; D046911:Macromolecular Substances; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D002215:Captan; D014544:Uridine Triphosphate