Effect of polysaccharide interactions on antibiotic susceptibility of Pseudomonas aeruginosa.
The relative viscosity of Pseudomonas aeruginosa alginate was shown to increase markedly when combined with mucin, Ca2+ ions and the exopolysaccharide from Pseudomonas cepacia. The presence of such a heterodisperse polysaccharide solution significantly reduced the diffusion and hence antimicrobial activity of tobramycin and to a lesser extent ciprofloxacin against Ps. aeruginosa by factors of 90 and 2.5-fold respectively over a 5 h incubation period. The clinical implications of these results are discussed in relation to cystic fibrosis.
D016956:Burkholderia cepacia / Q000737:chemistry; D002412:Cations / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology*; D004347:Drug Interactions; D004352:Drug Resistance, Microbial; D008826:Microbial Sensitivity Tests; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D014031:Tobramycin / Q000494:pharmacology*; D014783:Viscosity / Q000187:drug effects
D002412:Cations; D011135:Polysaccharides, Bacterial; C064841:exopolysaccharide, Pseudomonas; D002939:Ciprofloxacin; D014031:Tobramycin