Non-histone protein synthesis during G1 phase and its relation to DNA replication.
The kinetics of non-histone chromosomal protein (NHCP) synthesis were studied in Chinese hamster ovary (CHO) plateau phase cells stimulated to proliferate and were compared to NHCP synthesis kinetics in two populations of synchronous G1 traversing cells. In all cases, NHCP synthesis rates increase 3- to 5-fold as cells traversed G1 and attained maximum values one hour before semi-conservative DNA replication began. Similar to results in synchronous G1 cells, the molecular weight distributions of the NHCP fraction from stimulated plateau phase cells underwent only minor changes, measured by sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis, as these cells moved toward S phase. Yet, during this progression after plateau phase and in the transition from early G1 to late G1 in synchronous cells, the total NHCP fraction increased significantly (1.5-2-fold) in amount per cell. These data indicate that plateau phase cells are similar to early G1 cells both in terms of their amounts of non-histone per cell and in their subsequent NHCP synthesis kinetics as they move toward S phase. These results extend previous findings which suggested that NHCP synthesis was coupled to DNA replication and demonstrate that the increased NHCP synthesis and accumulation in chromatin may be a biochemical marker for G1 progression.
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival; D004261:DNA Replication; D007930:Leucine / Q000378:metabolism; D008938:Mitosis; D008940:Mitotic Index; D008970:Molecular Weight; D009698:Nucleoproteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D013936:Thymidine / Q000494:pharmacology
D009698:Nucleoproteins; D007930:Leucine; D013936:Thymidine