Topographical distribution of dopaminergic innervation and of dopaminergic receptors in the rat striatum. I. Mictoestimation of [3H] dopamine uptake and dopamine content in microdiscs.
Topographical variations in the uptake of [3H] dopamine (DA) and in the endogenous content of DA were estimated in the striatum of the rat. For this purpose, microdiscs were punched out in serial 500 mum sections. [3H] DA uptake was measured in 0.25 M sucrose homogenates prepared from microdiscs punched out from frozen slices (--7C). This uptake was similar to that observed in fresh tissues. It was unaffected by desmethylimipramine (5 X 10(-7) M), inhibited by benztropine (10(-6) M) and no longer detectable after 6-hydroxydopamine-induced degeneration of the nigrostriatal dopaminergic pathway. Both [3H] DA uptake and DA content decreased regularly from the rostral to the caudal part of the structure. In contrast, no important differences could be found in the dorso-ventral plane. These results suggest that the extent of dopaminergic innervation is heterogenous within the structure.
D000818:Animals; D001590:Benztropine / Q000494:pharmacology; D001923:Brain Chemistry; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D008297:Male; D051381:Rats; D011941:Receptors, Adrenergic
D011941:Receptors, Adrenergic; D001590:Benztropine; D004298:Dopamine