title: Effects of Na+ and other monovalent cations on Ca-efflux from synaptosomes.
abstract: Effects of monovalent cations on Ca-efflux were examined in rat brain cortex slices, synaptosomes and synaptic plasma membranes. Effluxes of 45Ca from brain slices and synaptosomes were stimulated by Na+ in medium and the effects of Na+ on the 45Ca-effluxes disappeared at low temperature. Furthermore, we observed that Rb+ had more effect than Na+ on 45Ca-efflux from the synaptosomes, while Li+ had less effect. On the other hand, release of 45Ca from the synaptic plasma membranes which had been preincubated with ATP, MG++ and 45Ca was stimulated by Na+ and Li+. But Cs+ and Rb+ were less effective on the release. These results indicate occurrence of Na-dependent Ca-efflux at nerve endings. However, the assumption that ATP-dependent Ca-binding with synaptic plasma membranes may be a partial reaction of Ca-efflux was not supported by these experiments.
mesh_terms: D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002462:Cell Membrane / Q000378:metabolism; D066298:In Vitro Techniques; D008670:Metals / Q000494:pharmacology*; D051381:Rats; D012964:Sodium / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002132:Calcium Radioisotopes; D008670:Metals; D012964:Sodium; D002118:Calcium