Effect of combination treatment with cyclophosphamide and nonspecific passive immunization on a transplantable tumor in WKA rats.
Combination of cyclophosphamide (CY) and passive immunization with lymphoid cells sensitized to allogeneic tumor was studied in the treatment of a methylcholanthrene-induced transplantable fibrosarcoma in WKA rats. For determination of the most effective timing of combination treatment, rats given an injection of CY on Day 3 received passive transfer of the sensitized lymphoid cells on Day 0, 1, 2, 3, 4, or 6. A remarkable therapeutic effect was observed only when the passive transfer was combined on Day 4 with CY on Day 3. Rats inoculated with tumor succumbed in all cases without any treatment. After i.v. injection of CY on Day 3, 2 of 28 rats were cured (7.1%). Passive immunization with the sensitized lymphoid cells on Day 4 resulted in no therapeutic effect. After combination of CY on Day 3 and transfer of nonsensitized normal lymphoid cells on Day 4, 2 of 15 rats survived (13.3%). However, combination of CY and passive transfer of the nonspecifically sensitized lymphoid cells resulted in 23 survivors of 29 rats (79.3%).
D000818:Animals; D003520:Cyclophosphamide / Q000276:immunology / Q000627:therapeutic use*; D005260:Female; D005354:Fibrosarcoma / Q000188:drug therapy / Q000628:therapy; D007111:Immunity, Cellular; D007112:Immunity, Maternally-Acquired; D007114:Immunization; D007165:Immunosuppression Therapy; D007167:Immunotherapy; D008221:Lymphoid Tissue / Q000276:immunology; D008297:Male; D009374:Neoplasms, Experimental / Q000628:therapy*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D003520:Cyclophosphamide