Critical importance of citrate--blood ratio in platelet aggregation studies.
The effects of citrate concentration on adenosinediphosphate-, epinephrine-, collagen-, and ristocetin-induced human platelet aggregation were investigated. Relatively small increments in citrate concentration markedly inhibited platelet aggregation by all three physiologic agents. The inhibitory effect was greatest on epinephrine-induced aggregation, and least on collagen-induced aggregation. Ristocetin-induced aggregation was not affected by excess citrate anticoagulation. These findings indicate the importance of controlling the citrate:blood ratio in clinical platelet aggregation studies and in the assessment of antiplatelet drugs.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D002951:Citrates / Q000097:blood*; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects; D012310:Ristocetin / Q000494:pharmacology; D013997:Time Factors
D002951:Citrates; D012310:Ristocetin; D000244:Adenosine Diphosphate; D003094:Collagen; D004837:Epinephrine