Tunicamycin-induced inhibition of functional expression of glutamate receptors in Xenopus oocytes.
The effects of tunicamycin, a specific inhibitor of N-linked glycosylation, on functional expression of glutamate receptor subtypes were investigated in RNA-injected oocytes. In the presence of tunicamycin the expression of ligand-operated receptors sensitive to kainate, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) and quisqualate were completely blocked. The inhibitory effect was reversible after removal of tunicamycin from the culture medium.
D000818:Animals; D004594:Electrophysiology; D018691:Excitatory Amino Acid Antagonists; D007051:Ibotenic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology; D009844:Oligosaccharides / Q000378:metabolism; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D016318:Quisqualic Acid / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D017470:Receptors, Glutamate / Q000096:biosynthesis; D014415:Tunicamycin / Q000494:pharmacology*; D014982:Xenopus laevis; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
D018691:Excitatory Amino Acid Antagonists; D007473:Ion Channels; D009844:Oligosaccharides; D017470:Receptors, Glutamate; D014415:Tunicamycin; D007051:Ibotenic Acid; D012313:RNA; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D016318:Quisqualic Acid; D007608:Kainic Acid