Selective modification of cell surface proteins and thymidine transport in hamster cells exposed to cholera toxin.
The increased adherence and morphological response which occurs in Chinese hamster ovary cells as a result of exposure to cholera toxin is paralleled by modification in the relative exposure of outer proteins. Mild proteolysis treatment of the cells prelabeled with [3H] glucosamine reveals a markedly different kinetics of release of external glycopeptides as a result of exposure to cholera toxin. Selective alterations in external tyrosyl-rich proteins can also be detected by lactoperoxidase-catalyzed radioiodination. The above modifications are accompanied by a decrease in the rate of thymidine uptake by toxin-treated cells.
D001693:Biological Transport, Active / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D006023:Glycoproteins; D011506:Proteins; D013936:Thymidine / Q000378:metabolism*; D014118:Toxins, Biological / Q000494:pharmacology*; D014734:Vibrio cholerae
D006023:Glycoproteins; D011506:Proteins; D014118:Toxins, Biological; D013936:Thymidine