Immunodiagnosis of human dirofilariasis by enzyme-linked immunosorbent assay using recombinant DNA-derived fusion protein.
An enzyme-linked immunosorbent assay (ELISA) using antigenic beta-galactosidase-Dirofilaria immitis recombinant fusion protein (FP) obtained by the recombinant DNA technique provided a useful diagnostic tool for human dirofilariasis. D. immitis-infected human sera reacted strongly with FP that was immobilized with anti-beta-galactosidase monoclonal antibody on microplates. However, the FP did not react with sera from patients with other filariasis. In detection of anti-D. immitis IgG antibody. ELISA using FP was highly sensitive and specific compared to that using crude somatic antigen.
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth; D003429:Cross Reactions; D004183:Dirofilaria immitis / Q000276:immunology*; D004184:Dirofilariasis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006801:Humans; D011993:Recombinant Fusion Proteins; D012680:Sensitivity and Specificity; D001616:beta-Galactosidase / Q000276:immunology
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase