title: Brain nitric oxide synthase is a haemoprotein.
abstract: Brain nitric oxide (NO) synthase showed pyridine haemochrome spectra typical of ferroprotoporphyrin IX-containing enzymes. The haem content of purified NO synthase was in the range 0.7-0.9 mol/mol of 160 kDa subunit. In the presence of CO, NO, KCN and miconazole, the L-citrulline-forming activity of NO synthase was markedly diminished, demonstrating that enzyme-bound haem is involved in enzymic NO synthesis.
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002248:Carbon Monoxide / Q000494:pharmacology; D002956:Citrulline / Q000378:metabolism; D006418:Heme / Q000032:analysis / Q000378:metabolism*; D008825:Miconazole / Q000494:pharmacology; D009569:Nitric Oxide / Q000494:pharmacology; D019001:Nitric Oxide Synthase; D011190:Potassium Cyanide / Q000494:pharmacology; D013053:Spectrophotometry; D013552:Swine
chemical_list: D002956:Citrulline; D009569:Nitric Oxide; D006418:Heme; D008825:Miconazole; D002248:Carbon Monoxide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D011190:Potassium Cyanide