A competitive protein binding assay for plasma 25-hydroxyvitamin D3 in normal children.
In order to determine the plasma level of 25-hydroxyvitamin D3 (25-OH-D3) in Japanese children by the competitive protein binding assay, we studied binding proteins in D-deficient rats, and obtained the following results. 1) By using serum and kidney cytosol of D-deficient rats, we could obtain a standard curve with high sensitivity and accuracy which enabled us to determine the 25-OH-D3 level ranging from 0.5 to 5.0 ng. 2) The plasma 25-OH-D3 level was found to be 21.6+/-10.1 ng/ml (n=17) in healthy infants and children from 1 to 15 year-old, and 11.4+/-8.6 ng/ml (n=27) in mature neonates up to 2 days after delivery.
D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D002675:Child, Preschool; D002784:Cholesterol; D003348:Cortisone; D003429:Cross Reactions; D005260:Female; D006801:Humans; D006887:Hydroxycholecalciferols / Q000097:blood*; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy; D011374:Progesterone; D011869:Radioligand Assay; D051381:Rats; D014807:Vitamin D; D014808:Vitamin D Deficiency / Q000097:blood
D006887:Hydroxycholecalciferols; D014807:Vitamin D; D011374:Progesterone; D002784:Cholesterol; D003348:Cortisone