Quantitation of labeled globin messenger RNA by hybridization with excess complementary DNA covalently bound to cellulose.
A method is described to quantitate labeled globin mRNA by hybridization with excess cDNA which was enzymatically polymerized on oligo(dT)-cellulose. In a large excess of cDNA-cellulose the rate of RNA hybridization was dependent on DNA concentration and not on RNA concentration. Nonhybridized RNA can be digested by RNase and washed from the cDNA which is covalently bound to cellulose. This enables the detection of labeled globin mRNA even when present in a porportion as low as 0.02-0.03% of the total RNA.
D000818:Animals; D001667:Binding, Competitive; D001921:Brain; D002482:Cellulose / Q000378:metabolism*; D005914:Globins / Q000096:biosynthesis*; D006358:Hot Temperature; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
D012333:RNA, Messenger; D005914:Globins; D002482:Cellulose