[Target epitopes of monoclonal antibodies against ABH structures].
Comparing the reactivities of murine and human monoclonal antibodies directed against the antigens of A, B, and H blood groups by using different techniques (agglutination, inhibition by synthetic oligosaccharides and salivary antigens, tissue immunofluorescence, the use of an anti-idiotype antibody) enabled us to show a high heterogeneity of anti-A, anti-AB antibodies and, to some extend, anti-B and anti-H antibodies. Despite this diversity, the determination of the antibody specificity thanks to synthetic antigens made it possible to distinguish several groups of anti-A, B, AB, and H antibodies and to establish a classification that takes also their reactivity towards salivary and tissue antigens into account. On the contrary, few correlations were found between these tests and the ability of antibodies to agglutinate red blood cells of the different ABO-system variants.
D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001788:Blood Grouping and Crossmatching / Q000379:methods; D002240:Carbohydrate Sequence; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D004910:Erythrocyte Membrane / Q000276:immunology; D005643:Fucose; D006031:Glycosylation; D006386:Hemagglutination Tests; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000276:immunology; D010641:Phenotype; D012471:Salivary Proteins and Peptides / Q000276:immunology; D001743:Urinary Bladder / Q000276:immunology
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D012471:Salivary Proteins and Peptides; D005643:Fucose
Les épitopes cibles des anticorps monoclonaux dirigés contre les structures ABH.