Yolk vitronectin. Purification and differences from its blood homologue in molecular size, heparin binding, collagen binding, and bound carbohydrate.
This is the first report on a unique vitronectin molecule, yolk vitronectin, which is similar to its blood homologue in cell spreading activity but different in molecular size, bound carbohydrate, and heparin and collagen binding activity. Yolk vitronectin was purified 2,500-fold from chick egg yolk by a combination of hydroxylapatite, DEAE-cellulose, and anti-vitronectin-Sepharose column chromatographies. In SDS-polyacrylamide gel electrophoresis under reducing conditions, yolk vitronectin was separated into 54- and 45-kDa bands, which are 16 and 25 kDa smaller, respectively, than the 70-kDa major band of chick blood vitronectin. The 54-kDa band shares the same NH2-terminal sequence as chick blood vitronectin. In contrast, the NH2-terminal sequence of the 45-kDa band is somewhat homologous with the internal sequences of mammalian vitronectins beginning at the 50th amino acid from the NH2 terminus. The bound carbohydrate of the 54- and 45-kDa species of yolk vitronectin is similar to, but distinct from, that of blood vitronectin. Unlike blood vitronectin, yolk vitronectin cannot bind to either heparin or collagen.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001798:Blood Proteins / Q000302:isolation & purification*; D015153:Blotting, Western; D002241:Carbohydrates / Q000032:analysis; D002645:Chickens; D002845:Chromatography; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D003094:Collagen / Q000378:metabolism*; D017886:Durapatite; D004529:Egg White; D004530:Egg Yolk; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005353:Fibronectins / Q000302:isolation & purification* / Q000378:metabolism; D006023:Glycoproteins / Q000097:blood / Q000302:isolation & purification* / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D006882:Hydroxyapatites; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D019096:Vitronectin
D001798:Blood Proteins; D002241:Carbohydrates; D005353:Fibronectins; D006023:Glycoproteins; D006882:Hydroxyapatites; D019096:Vitronectin; D006493:Heparin; D003094:Collagen; D017886:Durapatite