A novel microcarrier bead model to investigate bone cell responses to mechanical compression in vitro.
Periosteal cells grown in macroporous microcarrier bead columns were cyclically loaded with compressive force using a newly developed model. In response to 1/2 h of cyclic compression, RNA synthesis increased significantly by twofold, from 113.4 +/- 19.6 to 260.7 +/- 363 (p < 0.01) after 16 h, whereas DNA synthesis did not increase significantly after 24 h. The microcarrier bead model was calibrated using a linear volume displacement transducer with a range of strain magnitudes applied across the column of 1000-6000 microstrain.
D000818:Animals; D002138:Calibration; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D008863:Microspheres; D010521:Periosteum / Q000166:cytology / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical
D012313:RNA; D004247:DNA