title: [Primary cell cultures of leukosis P-388 as a possible model for the screening of antitumor substances].
abstract: A substrain of the ascitic leucosis P-388 was obtained as a result of interchanging passages of P-388 leucosis cells in the primary cultures and abdominal cavity of mice. The tumor cells of the ascitic leucosis P-388 multiplied in the primary suspended culutres as well as in vivo. The substrain lost its hemorrhagic properties. The content of DNA and RNA in the primary cultures of P-388 doubled every 16-18 hours and the number of the cells doubled every 24-26 hours. The cells of the adapted substrain of P-388 grew also in the semiliquid agarized medium forming compact colonies by the 4th-5th day of cultivation. The primary suspended cultures of P-388 were highly sensitive to cytostatics and in particular to vinblastin and kolchamine (alkaloids). In this connection they were recommended for prescreening antitumor compounds.
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001202:Ascitic Fluid / Q000166:cytology / Q000187:drug effects; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D004351:Drug Resistance; D007942:Leukemia, Experimental / Q000188:drug therapy*; D051379:Mice; D012334:RNA, Neoplasm / Q000096:biosynthesis; D013997:Time Factors; D014586:USSR
chemical_list: D000903:Antibiotics, Antineoplastic; D003470:Culture Media; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm
vernacular_title: Pervichnye kul'tury kletok leĭkoza P-388 kak vozmozhnaia model' dlia skrininga protivoopukholevykh veshchestv