Dot blot immunoassay for detection of human semen.
A sensitive and specific dot blot immunoassay based on prostate specific antigen (PSA) antibodies and a radiolabelled Protein A detection system was developed for detection of trace amounts of human semen in stains. The method was found highly sensitive and detected semen stain extracts diluted 10,000 times. Semen stains up to five years old were successfully detected by the method. The assay is found to be highly suitable for semen detection in forensic analysis.
D005069:Evaluation Studies as Topic; D006801:Humans; D015151:Immunoblotting / Q000379:methods* / Q000706:statistics & numerical data; D008297:Male; D017430:Prostate-Specific Antigen / Q000032:analysis; D012661:Semen / Q000737:chemistry* / Q000276:immunology; D012680:Sensitivity and Specificity; D013205:Staphylococcal Protein A
D013205:Staphylococcal Protein A; D017430:Prostate-Specific Antigen