A rapid, simple and sensitive method for the demonstration of central catecholamine-containing neurons and axons by glyoxylic acid-induced fluorescence. II. A detailed description of methodology.
The glyoxylic acid-induced fluorescence method for localization of brain catecholamine neurons has been modified. Fluorescence is developed rapidly in cryostat sections of brains fixed by perfusion with 0.5% depolymerized paraformaldehyde and 2.0% glyoxylic acid. Since neither freeze drying nor vibratome sectioning is required, total processing time can be less than 1 hr. Both perikarya and fine varicose axons of norepinephrine- and dopamine-containing neurons can be seen throughout the neuroaxis. The modified technique retains good cytologic integrity and may provide a useful alternative for methods combining histochemical approaches.
D000818:Animals; D001369:Axons / Q000032:analysis / Q000648:ultrastructure*; D001665:Binding Sites; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis*; D005260:Female; D006038:Glyoxylates; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D009474:Neurons / Q000032:analysis / Q000648:ultrastructure*; D009638:Norepinephrine / Q000032:analysis; D051381:Rats
D002395:Catecholamines; D006038:Glyoxylates; D009638:Norepinephrine