[EBV-lymphadenitis: diagnostic possibilities using immunohistochemistry and in situ hybridization].
EBV infections were studied in paraffin embedded lymph node specimens (n = 20) on DNA, protein, and histological level. The lymph nodes were obtained from 20 patients all testing positive for EBV serologically. The EBV DNA was detected by non radioactive in-situ-hybridisation using the BamHI W fragment of the virus. The EBV latent membrane protein was detected immunohistochemically using the LMP antibody (DAKO). Infected cells were characterized by monoclonal B cell antibodies. By in-situ-hybridization we found positive signals for EBV in all specimens. In contrast, no LMP was detectable immunohistochemically in 2/20 lymph nodes.
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D008198:Lymph Nodes / Q000382:microbiology* / Q000473:pathology; D008199:Lymphadenitis / Q000382:microbiology* / Q000473:pathology*; D015183:Restriction Mapping
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens
EBV-Lymphadenitis: Diagnostische Möglichkeiten mit Immunhistochemie und in-situ-Hybridisierung.