title: Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A260/A280 and on accuracy of mRNA quantitation by reverse transcriptase-PCR.
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D002725:Chloroform; D004247:DNA / Q000032:analysis*; D004730:Endothelium, Vascular / Q000166:cytology; D019791:Guanidine; D006146:Guanidines; D006801:Humans; D008969:Molecular Sequence Data; D019800:Phenol; D010636:Phenols; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D006146:Guanidines; D010636:Phenols; D012333:RNA, Messenger; D012997:Solvents; D019800:Phenol; D002725:Chloroform; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D019791:Guanidine