A preliminary map of epitopes on envelope glycoprotein E1 of HCV strain Brescia.
Monoclonal antibodies (MAbs) directed against envelope glycoprotein E1 (gp51-54) of hog cholera virus (HCV) strain Brescia have been shown to recognize four different antigenic domains A, B, C and D. Epitopes of within domain A have mainly been found conserved among HCV strains, whereas epitopes within domains B, C and D are not conserved. We used transiently expressed hybrid E1 genes of HCV strains Brescia and "C" to map the non-conserved epitopes on E1. Epitopes in domains B and C are located within the ultimate N-terminal 104 amino acids. The non-conserved subdomain A3 is most probably located between domains B/C and a hydrophobic region, which is highly conserved between HCV strains Brescia and "C". The conserved subdomains A1 and A2 are probably located in the vicinity and C-terminally of this conserved, hydrophobic region, which is near the centre of the E1 amino acid sequence.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology*; D004279:DNA, Viral / Q000737:chemistry; D000939:Epitopes / Q000032:analysis / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D004279:DNA, Viral; D000939:Epitopes; D006023:Glycoproteins; D009838:Oligodeoxyribonucleotides; D014759:Viral Envelope Proteins