The distribution of 14c from [U-14c]glucose in mice using whole-body autoradiography.
Tissue distribution of radioactive carbon from [U-14C]glucose in the mouse in vivo was studied by whole-body autoradiography. The mice were frozen with Dry-Ice-acetone at 0.5, 1, 5 and 30 min, 1 and 24 hr and 1 and 3 weeks after intraperitoneal injection of [U-14C]glucose. Whole-sagittal sections of the frozen mouse, obtained by using a microtome in a cryostat, were dried in a cryostat and autoradiographed. The resulting dry autoradiographs are called untreated autoradiographs in the present work. The sections were then fixed in cold 6% (w/v) HClO4, dried at room temperature and again autoradiographed. Autoradiographs that have undergone this process are referred to as treated autoradiographs. In both untreated and treated autoradiographs, within 1 min following injection of the labeled glucose, the abdominal cavity had the highest autoradiographic density. At 1 hr, density became highest in Harder's, sublingual and duodenal glands, large intestinal mucosa and tongue, and after 3 weeks, no autoradiographic denisty was present.
D002001:Adipose Tissue, Brown / Q000378:metabolism; D000818:Animals; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D004386:Duodenum / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D012469:Salivary Glands / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D013997:Time Factors; D014059:Tongue / Q000378:metabolism
D002250:Carbon Radioisotopes; D005947:Glucose