Inhibitory effects of pectic substances on activated hyaluronidase and histamine release from mast cells.
In this paper, we report the effect of pectic substances and D-galacturonic acid, the main constituent of pectic substances, on activated hyaluronidase and histamine release from mast cells. Although D-galacturonic acid itself showed no inhibition, IC50 values of hyaluronidase inhibition were correlated with the D-galacturonic-acid content of pectic substances. It was thought that the polymerization of D-galacturonic acid was necessary for inhibition of activated hyaluronidase. This type of inhibition was suggested to be non-competitive by the Lineweaver-Burk plot. Furthermore, pectic substances, including those purified from Gymnema sylvestre, inhibited histamine release from isolated rat peritoneal mast cells, which had been induced by the antigen. These results suggest that pectic substances may have anti-allergic activities.
D000818:Animals; D004789:Enzyme Activation; D006603:Hexuronic Acids / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010368:Pectins / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar
D006603:Hexuronic Acids; C007819:galacturonic acid; D010368:Pectins; D006821:Hyaluronoglucosaminidase