Physical aspects and cytoplasmic distribution of messenger RNA in mouse kidney.
As a prerequisite to examining mRNA metabolism in compensatory renal hypertrophy, polyadenylated RNA has been purified from normal mouse kidney polysomal RNA by selection on oligo(dT)-cellulose. Poly(A)-containing RNA dissociated from polysomes by treatment with 10 mM EDTA and sedimented heterogeneously in dodecyl sulfate-containing sucrose density gradients with a mean sedimentation coefficient of 20 S. Poly(A) derived from this RNA migrated at the rate of 6-7 S RNA in dodecyl sulfate-containing 10% polyacrylamide gels. Coelectrophoresis of poly(A) labeled for 90 min with poly(A) labeled for 24 h indicated the long-term labeled poly(A) migrated faster than pulse-labeled material. Twenty percent of the cytoplasmic poly(A)-containing mRNA was not associated with the polysomes, but sedimented in the 40-80 S region (post-polysomal). Messenger RNA from the post-polysomal region had sedimentation properties similar to those of mRNA prepared from polysomes indicating post-polysomal mRNA was not degraded polysomal mRNA. Preliminary labeling experiments indicated a rapid equilibration of radioactivity between the polysomal and post-polysomal mRNA populations, suggesting the post-polysomal mRNA may consist of mRNA in transit to the polysomes.
D000225:Adenine / Q000378:metabolism; D000818:Animals; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000032:analysis; D007668:Kidney / Q000032:analysis* / Q000378:metabolism; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009963:Orotic Acid / Q000378:metabolism; D011061:Poly A / Q000032:analysis; D011132:Polyribosomes / Q000032:analysis / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis; D051381:Rats
D012333:RNA, Messenger; D011061:Poly A; D009963:Orotic Acid; D000225:Adenine