Radiation-induced aneusomic clones in bone marrow of rats.
Wistar rats 3 months old were given a single whole-body X-irradiation with 700 R. They were killed 9.3 months, on average, after irradiation. From the bone marrows of the 23 irradiated rats, 54 clones of cells with radiation-induced chromosome abnormalities, ranging from 3.3 to 78.3% in size, were obtained. Karyotype analysis at the banding level showed that 43 out of the 54 clones had balanced chromosome constitutions, and that the remaining 11 clones were unbalanced. The 43 balanced clones consisted of 33 clones with reciprocal translocations, 6 with inversions and 4 with both translocations and inversions. The 11 unbalanced clones were made up of 7 aneuploid clones and 4 pseudo-diploid clones. Of the 54 clones, 15 were large with frequencies of more than 25%. Contrary to general belief that cells with unbalanced chromosome constitutions have less capacity to proliferate than those with balanced ones, 8 of the 15 large clones, especially all, except 1, of the largest 6 clones were unbalanced, either aneuploid or pseudo-diploid.
D000782:Aneuploidy; D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects*; D001854:Bone Marrow Cells; D002470:Cell Survival; D002875:Chromosomes; D002999:Clone Cells / Q000648:ultrastructure; D005260:Female; D007621:Karyotyping; D008297:Male; D011831:Radiation Genetics; D051381:Rats; D013997:Time Factors; D014965:X-Rays