Rapid purification of a high molecular weight human brain protein by chromatography on controlled pore glass.
Controlled pore glass (CPG) chromatography was employed to simply (one pass through column) and rapidly (60 minutes) purify a human brain specific protein having a high molecular weight (approximately 250,000 mol. wt.) from a crude brain extract containing proteins of varying molecular weights. This method, either exactly as described herein or by adjusting the pore size of the CPG, should be adaptable to other purification problems.
D001923:Brain Chemistry; D002845:Chromatography / Q000379:methods; D004589:Electrophoresis, Disc; D005898:Glass; D006801:Humans; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*
D009419:Nerve Tissue Proteins