Measurement of HIV-1 reverse transcriptase by a nonradioactive assay system.
Reverse transcriptase activity was measured by incorporation of dUMP linked to digoxigenin into a suitable template-primer molecule. Incorporation was monitored by using peroxidase-conjugated Fab fragments directed against digoxigenin. The standard assay measuring incorporation of radiolabeled nucleotides into acid-precipitable material was compared with this new immunochemical assay with regard to its usefulness for testing inhibitors of reverse transcriptase.
D004076:Digoxigenin; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology*; D012194:RNA-Directed DNA Polymerase / Q000032:analysis*; D018894:Reverse Transcriptase Inhibitors
D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; D004076:Digoxigenin