Novel cell junctions induced by activating Thy-1-specific antibodies.
KT16, like other anti-Thy-1 antibodies, induces T cell aggregation. Protein A-gold labelling shows the antibody to be concentrated along areas of intercellular contacts. Electron micrographs of KT16 treated T cells reveal a consistent type of junction between the cells. We demonstrate that this type of cell junction is Thy-1 specific, is predominantly the property of antibodies directed against a particular epitope, and is distinct from cellular aggregation caused by concanavalin A or anti-CD3 antibodies. The degree of adhesiveness induced by different anti-Thy-1 antibodies is related to their mitogenic capacity.
D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D000954:Antigens, Surface / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology; D002449:Cell Aggregation; D002455:Cell Division; D003208:Concanavalin A / Q000494:pharmacology; D000939:Epitopes / Q000276:immunology; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000648:ultrastructure; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D016253:Microscopy, Immunoelectron; D013601:T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D018800:Thy-1 Antigens
D000906:Antibodies; D000954:Antigens, Surface; D017252:CD3 Complex; D000939:Epitopes; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens; D003208:Concanavalin A