Isolation of regulatory mutants of Pseudomonas acidovorans by use of amino acid analogs.
Mutants resistance to various combinations of threonine, lysine and/or their analogs were obtained and characterized in Pseudomonas acidovorans. In particular, mutants resistant to aminoethylcysteine had a dihydrodipicolinate synthetase insensitive to lysine inhibition whereas mutants resistant to threonine plus a low concentration of aminoethylcysteine had a feedback-insensitive aspartokinase.
D000494:Allosteric Regulation; D000596:Amino Acids / Q000096:biosynthesis*; D001222:Aspartate Kinase / Q000037:antagonists & inhibitors; D003545:Cysteine / Q000031:analogs & derivatives; D006715:Homoserine Dehydrogenase / Q000037:antagonists & inhibitors; D006836:Hydro-Lyases / Q000037:antagonists & inhibitors; D008239:Lysine / Q000096:biosynthesis; D009154:Mutation; D011549:Pseudomonas / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism; D013912:Threonine / Q000096:biosynthesis; D014633:Valine / Q000031:analogs & derivatives
D000596:Amino Acids; D013912:Threonine; D006715:Homoserine Dehydrogenase; D001222:Aspartate Kinase; D006836:Hydro-Lyases; D014633:Valine; D008239:Lysine; D003545:Cysteine