Inhibition of repair of bleomycin-induced DNA strand breaks by 2'-deoxycoformycin and its effect on antitumor activity in L5178Y lymphoblasts.
We have observed previously that treatment of plateau-phase L5178Y murine lymphoblasts in vitro with 2'-deoxycoformycin plus deoxyadenosine (dCF/dAdo) can inhibit the repair of X-irradiation-induced DNA single-strand breaks (SSB) in these cells and that this effect is associated with synergistic cell kill. In this study we examined the effect of a combination treatment of plateau-phase L5178Y cells with bleomycin (BLM) plus dCF/dAdo. Incubation of BLM-treated cells with dCF/dAdo resulted in significant inhibition of the repair of BLM-induced DNA SSB. However, an additive, but not a synergistic, increase in cell kill was observed when cells were treated with a combination of BLM plus dCF/dAdo.
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D001761:Bleomycin / Q000633:toxicity*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000187:drug effects* / Q000502:physiology; D004277:DNA, Single-Stranded / Q000187:drug effects; D003839:Deoxyadenosines / Q000494:pharmacology; D004357:Drug Synergism; D007940:Leukemia L5178 / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D051379:Mice; D008811:Mice, Inbred DBA; D015649:Pentostatin / Q000494:pharmacology*
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D003839:Deoxyadenosines; D001761:Bleomycin; D015649:Pentostatin