Intrasplenic tumor formation in the syrian hamster (Mesocricetus auratus) after tracheal implants and treatment with diethylnitrosamine.
In two simultaneous experiments (A and B), tracheal tissue from 25 young Syrian hamsters was implanted in their mothers' spleens. In A, after tracheas from 3-week-old untreated donor offspring were implanted into their mothers, the mothers received 18 mg diethylnitrosamine (DEN)/kg body weight, sc, twice weekly for 20 weeks. Thereafter, the implants were removed and transferred to the spleens of other untreated offspring that were then similarly treated with DEN for 20 weeks. In B, the procedure was similar except that the donor offspring were pretreated with DEN for 20 weeks, beginning at the age of 8 weeks. The average treatment time for the implants in experiment A was 65 weeks; in B, 44 weeks. In A, only four implants demonstrated a papilloma or a papillary polyp. In B, such neoplasms developed after a much shorter treatment time, and in three cases, squamous cell carcinomas with invasive growth in to the splenic pulp were formed.
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000473:pathology; D004052:Diethylnitrosamine; D005260:Female; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009602:Nitrosamines; D010212:Papilloma / Q000139:chemically induced* / Q000473:pathology; D013154:Spleen / Q000601:surgery; D013997:Time Factors; D014132:Trachea / Q000637:transplantation; D014134:Tracheal Neoplasms / Q000139:chemically induced* / Q000473:pathology
D009602:Nitrosamines; D004052:Diethylnitrosamine