title: Biosynthesis of diacylglycerols by rat intestinal mucosa in vivo.
abstract: The biosynthesis of diacylglycerols was studied in rat intestinal mucosa during in vivo absorption of a low molecular weight fraction fraction of butter oil and of the corresponding medium and long chain fatty acids. The experimental fat solutions were given by stomach tube to the animals after a 24-h fast and mucosal scraping were collected 3 h later. The lipids were isolated and the acylclycerols determined by combined thin-layer chromatography gas-liquid chromatography techniques and stereospecific analyses. Free fatty acid feeding led mainly to sn-1,2-diacyl-glycerols, which contained exogenous and endogenous fatty acids. During triacylglycerol feeding, both sn-1,2-and sn-2,3-diacylglycerols were recovered in significant amounts from the intestinal mucosa. The composition of the sn-2,3-diacylglycerols corresponded to that with exogenous fatty acids but the sn-1,2-diacylglycerols clearly contained both exogenous and endogenous fatty acids. In all cases it was possible to isolate endogenous sn-1,2-diacylglycerols made up largely of species with linoleic and arachidonic acids in the 2 position and palmitic and stearic acids in the 1 position, which apparently were not converted to triacylglycerols. The in vivo reacylation of 2-monoacylglycerols via both sn-1,2- and sn-2,3-diacylglycerols is in agreement with similar findings in vitro with everted sacs of rat intestinal mucosa.
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D004041:Dietary Fats; D004075:Diglycerides / Q000096:biosynthesis*; D005215:Fasting; D005227:Fatty Acids / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D009821:Oils / Q000378:metabolism; D051381:Rats
chemical_list: D004041:Dietary Fats; D004075:Diglycerides; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D009821:Oils