The derivatization of oxidized polysaccharides for protein immobilization and affinity chromatography.
The present report describes the preparation of modified polysaccharides matrices useful for the synthesis of affinity adsorbents and immobilized proteins. Hydrazido-matrices were synthesized by condensing an excess of the bifunctional reagent, adipic acid dihydrazide, with periodate oxidized cellulose paper, Sephadex, or Sepharose matrices. Ribonucleotide dialdehyde cofactors, glyceraldehyde 3-phosphate, pyridoxal 5'-phosphate and oxidized DNAase B were separately bound to the hydrazido-polymers. Azido-matrices obtained by modification of the hydrazido-derivatives were coupled to specific amino ligands such as amino acids and proteins. Several adsorbents were prepared and used as models for affinity chromatography.
D000447:Aldehydes / Q000138:chemical synthesis; D001386:Azides / Q000138:chemical synthesis; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D002918:Chymotrypsin / Q000302:isolation & purification; D006834:Hydrazines / Q000138:chemical synthesis; D008024:Ligands; D008722:Methods; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000138:chemical synthesis*; D011506:Proteins / Q000302:isolation & purification*; D012265:Ribonucleotides / Q000138:chemical synthesis; D012685:Sepharose; D014357:Trypsin / Q000302:isolation & purification; D014361:Trypsin Inhibitors
D000447:Aldehydes; D001386:Azides; D006834:Hydrazines; D008024:Ligands; D011134:Polysaccharides; D011506:Proteins; D012265:Ribonucleotides; D014361:Trypsin Inhibitors; D012685:Sepharose; D002918:Chymotrypsin; D014357:Trypsin