In vitro inhibition of rat liver cholest-5en-3 beta-ol (cholesterol) biosynthesis by non-mercurial sulfhydryl reagents.
In vitro conversion of 2-14C-mevalonate to cholest-5en-3 beta-ol (cholesterol) in rat liver homogenates is inhibited by arsenite, beta-mercaptoethanol, dithiothreitol and ethanethiol. Two sterols containing 20 carbon atoms accumulate under these conditions. One of these is identified as 4,4 dimethyl-5alpha-cholest-8en-3beta-ol and the other tentatively identified as 4,4 dimethyl-5alpha-cholest-8,24-dien-3beta-ol. Based on these observations, these non-mercurial sulfhydryl reagents do not inhibit 5alpha-lanosta-8,24-dien-3beta-ol 14alpha demethylase.
D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D002784:Cholesterol / Q000096:biosynthesis*; D004229:Dithiothreitol / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D008798:Mevalonic Acid / Q000378:metabolism; D051381:Rats; D013261:Sterols / Q000096:biosynthesis; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D013261:Sterols; D013439:Sulfhydryl Reagents; D008623:Mercaptoethanol; D002784:Cholesterol; D001151:Arsenic; D008798:Mevalonic Acid; D004229:Dithiothreitol