Multi-recognition capability of E-selectin in a dynamic flow system, as evidenced by differential effects of sialidases and anti-carbohydrate antibodies on selectin-mediated cell adhesion at low vs. high wall shear stress: a preliminary note.
E-selectin has a "multi-recognition" capability in terms of epitope binding specificity, depending on adhesion conditions (static vs. low- or high-shear stress dynamic systems). Specifically, (i) adhesion based on expression of alpha 2-->3 sialylated Le(x) (SLe(x)) is prominent under static or low shear stress dynamic conditions; (ii) adhesion under high shear stress dynamic conditions does not depend on the known SLe(x) species, but rather on Lex with an adjacent unidentified sialosyl substitution, which shows different susceptibility to sialidases and antibodies compared to known SLe(x).
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D001173:Arthrobacter / Q000201:enzymology; D002240:Carbohydrate Sequence; D002448:Cell Adhesion / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000494:pharmacology*; D019040:E-Selectin; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D016256:Lewis X Antigen / Q000276:immunology*; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000494:pharmacology*; D009522:Newcastle disease virus / Q000201:enzymology; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D013314:Stress, Mechanical; D014407:Tumor Cells, Cultured; D014734:Vibrio cholerae / Q000201:enzymology
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D000939:Epitopes; D016256:Lewis X Antigen; D011994:Recombinant Proteins; D009439:Neuraminidase