[Expression of APO-1, a cell surface molecule mediating apoptosis, during normal B cell ontogeny and in B cell tumors. Co-expression and coregulation of APO-1 and ICAM-1 (CD54) in germinal central cells].
APO-1 is a 48kDa transmembrane glycoprotein and belongs to the NGF/TNF receptor family of surface molecules. Cross-linking of APO-1 induces apoptotic cell death in sensitive cells. Here we show that APO-1 is an activation molecule on B cells. It could be induced/enhanced on dense and buoyant tonsillar B cells, respectively, through surface immunoglobulin cross-linking in combination with interleukin-2 or by interferon-gamma together with tumor necrosis factor-alpha. These conditions also increased the amount of intercellular adhesion molecule-1 (ICAM-1; CD54) on these cells. Epstein-Barr virus transformants of peripheral B cells co-expressed APO-1 and CD54 at very high levels. Immunohistologically, APO-1 was detectable at low levels in a subpopulation of follicular center B blasts and, at higher levels, in sinusoidal B cells. APO-1 was undetectable in follicular mantle B cells and plasma cells. Neoplastic B cell essentially mimicked their reactive counterpart with regard to APO-1 and CD54 expression.
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000502:physiology*; D000951:Antigens, Neoplasm / Q000502:physiology*; D000954:Antigens, Surface / Q000502:physiology*; D017209:Apoptosis; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D002051:Burkitt Lymphoma / Q000276:immunology* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D018799:Intercellular Adhesion Molecule-1; D015448:Leukemia, B-Cell / Q000276:immunology* / Q000473:pathology; D007943:Leukemia, Hairy Cell / Q000276:immunology; D016393:Lymphoma, B-Cell / Q000276:immunology* / Q000473:pathology; D008565:Membrane Proteins / Q000502:physiology*; D014066:Palatine Tonsil / Q000276:immunology; D010954:Plasmacytoma / Q000276:immunology; D019014:fas Receptor
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D008565:Membrane Proteins; D019014:fas Receptor; D018799:Intercellular Adhesion Molecule-1
Expression von APO-1, eines Apoptose-vermittelnden Zelloberflächenmoleküls, während der normalen B-Zell-Ontogenese und in B-Zell-Tumoren. Koexpression und Koregulation von APO-1 und ICAM-1 (CD54) in Keimzentrumszellen.