The timing of reproductive cell development and movement in Schistosoma mansoni, S. japonicum, and S. haematobium, using techniques of autoradiography and transplantation.
Tritiated thymidine was incorporated into gonial and vitelline cells of male and female Schistosoma mansoni, S. japonicum, and S. haematobium after 2 hr of incubation. Spermatogonial cells developed into labeled mature sperm after 6 days of transplantation in all species. Insemination of females was detected on the same day indicating copulation is frequent. Oogonia labeled in the initial incubation matured into primary oocytes after 7 days of transplantation for S. mansoni and S. haematobium and after 6 days in S. japonicum. Cytoplasmic thymidine label was observed after 2-hr incubations in the primary oocytes of all species. Initial label in the vitellaria was scattered and heavy. After 3 days of transplantation, label was considerably diluted and by day 6 was undetectable in vitelline glands. These times for development and movement of reproductive cells are considered to be standards against which the effects of stressful conditions on the reproductive system can be assessed.
D000818:Animals; D002465:Cell Movement; D005260:Female; D007314:Insemination; D008297:Male; D009865:Oocytes / Q000166:cytology; D009867:Oogonia / Q000166:cytology; D010053:Ovary / Q000166:cytology; D012098:Reproduction; D012547:Schistosoma / Q000166:cytology / Q000502:physiology*; D012548:Schistosoma haematobium / Q000166:cytology / Q000502:physiology*; D012550:Schistosoma mansoni / Q000166:cytology / Q000502:physiology*; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology