title: Towards an understanding of viroid nature and replication.
abstract: In leaf strips and in nuclei isolated from potato spindle tuber viroid (PSTV)-infected tomato, incorporation of [3H]UMP into PSTV is shown to be inhibited by pre-treatment of the leaf-strips with actinomycin D. These results demonstrate that PSTV is synthesized in the cell nucleus and that replication may occur from a DNA template.
mesh_terms: D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004734:Energy Metabolism; D008274:Magnesium / Q000494:pharmacology; D008623:Mercaptoethanol / Q000494:pharmacology; D008937:Mitomycins / Q000494:pharmacology; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010942:Plant Viruses / Q000378:metabolism*; D010945:Plants, Edible / Q000187:drug effects / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D012328:RNA Viruses / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis*; D012260:Ribonucleases / Q000494:pharmacology; D012265:Ribonucleotides / Q000378:metabolism; D013698:Templates, Genetic; D014542:Uridine Monophosphate / Q000378:metabolism; D014779:Virus Replication
chemical_list: D008937:Mitomycins; D012367:RNA, Viral; D012265:Ribonucleotides; D003609:Dactinomycin; D008623:Mercaptoethanol; D010728:Phosphoenolpyruvate; D014542:Uridine Monophosphate; D011770:Pyruvate Kinase; D003851:Deoxyribonucleases; D012260:Ribonucleases; D008274:Magnesium; D004229:Dithiothreitol