Effect of leupeptin, a protease inhibitor, on induction of bladder tumors in rats by n-butyl-n-(4-hydroxybutyl)nitrosamine.
Leupeptin, isolated from Actinomycetes, is a potent and specific inhibitor of proteases. We found that the administration of leupeptin enhanced that size of urinary bladder tumors induced in rats by the oral administration of N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN). BBN was given as a 0.05% solution in the drinking water for 6 weeks, and then animals were fed a diet with or without 0.1% leupeptin for 30 weeks. The average weight of the bladders with tumors in rats fed a leupeptin diet was about eight times that of rats on a diet without leupeptin, though the incidences and average numbers of tumors in the bladders were similar in the two groups.
D000818:Animals; D002085:Butylhydroxybutylnitrosamine; D007976:Leupeptins / Q000494:pharmacology*; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009602:Nitrosamines; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced*
D007976:Leupeptins; D009602:Nitrosamines; D009842:Oligopeptides; D002085:Butylhydroxybutylnitrosamine