Inhibitors of plasminogen activation in human blood.
Plasma contained two inhibitors of plasminogen activation by urokinase when fractioned by gel filtration on Sephadex G-200. The inhibitor in the lower molecular weight fractions was separate from the principal protease inhibitors of plasma: alpha1-antitrypsin, alpha2-macroglobulin, C1 inactivator and antithrombin III, and from factor XIII. This activation inhibitor was present in both plasma and serum and its recovery was not reduced by preincubating the serum for 6 h at 37 degrees C. Its inhibitory activity was stable for several days at 4 degrees C, and was enhanced in the presence of an increased saline concentration. Preparations of the inhibitor, active in a clot lysis system, failed to inhibit the esterase activity of urokinase on N-alpha-acetyl-glycyl-L-lysine methyl ester.
D002850:Chromatography, Gel; D004791:Enzyme Inhibitors / Q000097:blood*; D004950:Esterases / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D010958:Plasminogen / Q000378:metabolism*; D011480:Protease Inhibitors; D012965:Sodium Chloride / Q000494:pharmacology; D014568:Urokinase-Type Plasminogen Activator / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D011480:Protease Inhibitors; D012965:Sodium Chloride; D010958:Plasminogen; D004950:Esterases; D014568:Urokinase-Type Plasminogen Activator