Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide.
Total nucleic acid extraction and selection of poly A-containing molecules yield preparative quantities of undegraded mRNA from adult and fetal pancreas. Using a stringent immunoassay, this mRNA is found to direct the synthesis of an immunoreactive insulin polypeptide in the wheat germ translation system. On sodium dodecyl sulfate polyacrylamide gels, this polypeptide (12,000-13,000 daltons) is larger than proinsulin (9,000 daltons).
D000375:Aging; D000818:Animals; D004285:Dogs; D005333:Fetus; D008970:Molecular Weight; D010179:Pancreas / Q000254:growth & development / Q000378:metabolism*; D010452:Peptide Biosynthesis; D011061:Poly A / Q000378:metabolism; D011233:Precipitin Tests; D011384:Proinsulin / Q000096:biosynthesis / Q000276:immunology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism; D014908:Triticum / Q000378:metabolism
D012333:RNA, Messenger; D011061:Poly A; D011384:Proinsulin