Deciphering the fluorescent variability of human genomic heterochromatin by DA/DAPI technique.
A number of selective staining techniques have been utilized to decipher the variability of pericentromeric heterochromatin. One such technique is called DA/DAPI and it is believed to be stain specific. However, we demonstrate otherwise and suggest that pericentromeric regions of all human chromosomes stain positive by DA/DAPI-technique. It must be emphasized that the incidence of DA/DAPI positive stained chromosomes, other than 1, 9, 15, 16 and Y, is a rare occurrence and only a small portion of the pericentromeric region is DA/DAPI positive, as reported here using 50 normal individuals.
D004214:Distamycins; D005260:Female; D005456:Fluorescent Dyes; D006570:Heterochromatin; D006801:Humans; D007211:Indoles; D007621:Karyotyping; D008297:Male; D013194:Staining and Labeling / Q000379:methods*
D004214:Distamycins; D005456:Fluorescent Dyes; D006570:Heterochromatin; D007211:Indoles; C007293:DAPI; C030000:stallimycin