title: Reevaluation of host corneal tissue 1955 to 1970: Calcofluor white staining for occult acanthamoebic infection.
abstract: The incidence of acanthamoebic keratitis before 1974 is not known. A retrospective study was conducted to understand better the early epidemiology of this infection. Host corneal tissue from therapeutic penetrating keratoplasties submitted to the Stanford Eye Pathology Laboratory from 1955 to 1970 were stained with Calcofluor white and observed by fluorescence microscopy. We reviewed 197 cases, and no misdiagnosed cases of acanthamoebic infection were found.
mesh_terms: D015823:Acanthamoeba Keratitis / Q000453:epidemiology / Q000473:pathology*; D001557:Benzenesulfonates; D002140:California / Q000453:epidemiology; D005456:Fluorescent Dyes; D006801:Humans; D015994:Incidence; D008856:Microscopy, Fluorescence / Q000379:methods; D012189:Retrospective Studies; D013194:Staining and Labeling
chemical_list: D001557:Benzenesulfonates; D005456:Fluorescent Dyes; C007061:C.I. Fluorescent Brightening Agent 28