title: Prolactin receptor messenger RNA is synthesized by the epithelial cells of the choroid plexus.
abstract: To identify cellular sites of prolactin receptor messenger RNA synthesis in the rat brain, we used a combined reverse transcriptase-polymerase chain reaction protocol to generate single stranded DNA probes for in situ hybridization. The results of these experiments identify the epithelial cells of the choroid plexus as a major site of prolactin receptor gene expression in the rat central nervous system.
mesh_terms: D000818:Animals; D002831:Choroid Plexus / Q000166:cytology / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D010053:Ovary / Q000502:physiology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D011981:Receptors, Prolactin / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D011981:Receptors, Prolactin; D012194:RNA-Directed DNA Polymerase