Inhibition of transcription by isonicotinic acid hydrazide.
Isonicotinic acid hydrazide (INH) reacts with the CMP moiety of a polynucleotide at slightly acid and alkaline pH. The reaction product, when used as a template in the cell-free transcription step, greatly diminishes the incorporation of GMP. In this system we were not able to show that INH -- in contrast with hydrazin -- is potentially capable of producing point mutations, since noncomplementary incorporation could not be observed with a poly(C)/INH adduct as template. Hydrazine could not be detected when INH was incubated with liver-cell fractions.
D001667:Binding, Competitive; D004247:DNA / Q000378:metabolism; D003864:Depression, Chemical; D066298:In Vitro Techniques; D007538:Isoniazid / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D011066:Poly C / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
D011066:Poly C; D012313:RNA; D004247:DNA; D007538:Isoniazid