title: Methylation analysis of glucosaminitol and glucosaminyl-glucosaminitol disaccharides. Formation of 2-deoxy-2-(N-acetylacetamido)-glucitol derivatives.
abstract: N-Acetylglucosaminitol, N-acetylglucosaminyl-beta 1',4-N-acetylglucosaminitol (chitobiitol), the corresponding trimer (chitotriitol) and N-acetylgucosaminyl-beta 1'6-N-acetylglucosaminitol were subjected to methylation analysis using combined gas-liquid chromatography/mass spectrometry. The preparation were subjected to permethylation according to Hakomori, followed by acid hydrolysis, reduction and peracetylation. From N-acetylglucosaminitol and its 4-0 and 6-0 substituted derivatives, methylated N-acetylacetamido derivatives were obtained. The possible conditions for the formation of these products are discussed.
mesh_terms: D000117:Acetylglucosamine; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D004187:Disaccharides; D005944:Glucosamine; D013058:Mass Spectrometry; D008745:Methylation; D013402:Sugar Alcohols
chemical_list: D004187:Disaccharides; D013402:Sugar Alcohols; D005944:Glucosamine; D000117:Acetylglucosamine