DiI as a marker for cellular transplantation into solid organs.
Research in cellular transplantation is frequently compromised by an inability to identify transplanted cells engrafting into orthotopic sites if they exhibit normal morphology and no unique antigenic markers. A method is described for using the fluorescent dye DiI as a marker for cell transplantation studies. This dye is not metabolized or exchanged between cells in vitro or in vivo and enables identification of engrafted cells by fluorescence microscopy, flow cytometry or fluorescence-activated cell sorting. Applications are described in autologous hepatocellular and thyroid follicular cell transplantation.
D000818:Animals; D002232:Carbocyanines; D002469:Cell Separation; D017690:Cell Transplantation; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D008099:Liver / Q000166:cytology; D012756:Sheep; D013194:Staining and Labeling / Q000379:methods*; D013961:Thyroid Gland / Q000166:cytology
D002232:Carbocyanines; D005456:Fluorescent Dyes; C024286:3,3'-dioctadecylindocarbocyanine