[Opportunistic malignant lymphomas in SIV infected primates--a model for Epstein-Barr virus associated lymphomas in AIDS].
In a series of 33 cynomolgus monkeys (Macaca fascicularis) experimentally infected with Simian Immunodeficiency virus (SIV), strain smm3, 13 animals developed malignant Non-Hodgkin lymphomas. These lymphomas presented with unusual primary manifestations like in the orbita, testes, and brain. The morphological features and immunophenotyping identified the tumors as high malignant B-cell lymphomas. In all tumors as well as in tumor-derived cell lines a cynomolgus B-lymphotropic herpes virus (CBLV) with structural homogeneity to the Epstein-Barr virus (EBV) could be demonstrated by Southern blotting with EBV-specific probes. The lymphoma cells also expressed CBLV-associated nuclear antigens involved in B-cell transformation crossreacting with EBNA-specific human sera and monoclonal antibodies. Ig-gene rearrangement studies revealed clonal populations, however, no translocations of the c-myc oncogene could be detected. The lymphomas developing with high frequency in SIV-induced immunodeficiency resemble a major subtype of human EBV-associated AIDS lymphomas. This animal model can therefore be used to further elucidate interactions of HIV and EBV in AIDS-related lymphomagenesis.
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D015342:DNA Probes; D004195:Disease Models, Animal; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D008223:Lymphoma / Q000209:etiology* / Q000382:microbiology; D008228:Lymphoma, Non-Hodgkin / Q000209:etiology* / Q000473:pathology; D008252:Macaca fascicularis; D008297:Male; D012190:Retroviridae; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications* / Q000473:pathology; D015302:Simian Immunodeficiency Virus; D014407:Tumor Cells, Cultured
D015342:DNA Probes
Opportunistische maligne Lymphome bei SIV-infizierten Primaten--Ein Modell für Epstein-Barr Virus assoziierte Lymphome bei AIDS.