A modified ortho-toluidine reagent for glucose analysis.
A micromethod for the determination  of glucose using a modified ortho-toluidine reagent is described. The proposed method correlates well with the original o-toluidine method. The reaction follows Beer's Law over a wide range of concentrations. The modified reagent contains 75 per cent less glacial acetic acid than the original ortho-toluidine reagent. The color intensity of the Schiff's base is two to three times higher than the original reagent.
D001786:Blood Glucose / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006801:Humans; D008832:Microchemistry / Q000379:methods*; D014052:Toluidines
D001786:Blood Glucose; D014052:Toluidines