Effect of calcium and anaerobiosis on the thermostability of Bacillus stearothermophilus.
Bacillus stearothermophilus NCA 2184 lost viability and subsequently released cytoplasmic components when suspended in 0.1 M tris(hydroxymethyl)aminomethane (Tris) buffer (pH 7.2) and incubated at 60 degrees C. Cell lysis was prevented by the addition of 10 mM CaCl2 to the Tris-buffer suspension. Cells which were incubated under anaerobic conditions for 20 min in the growth medium before they were collected were stable in the Tris-buffer suspension without added calcium. Anaerobic incubation effected an increase in membrane cardiolipin which appeared to be related to the increase in the thermostability of the cells.
D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology*; D002308:Cardiolipins / Q000096:biosynthesis; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005696:Galactosidases / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000187:drug effects / Q000378:metabolism; D006358:Hot Temperature; D010714:Phosphatidylethanolamines / Q000096:biosynthesis; D010715:Phosphatidylglycerols / Q000096:biosynthesis; D010758:Phosphorus / Q000378:metabolism
D001426:Bacterial Proteins; D002308:Cardiolipins; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010758:Phosphorus; D005696:Galactosidases; D002118:Calcium