Rescuing transgene expression by co-integration.
To test whether foreign gene expression can be improved in transgenic mice by manipulating the site of integration, we co-integrated the efficiently expressed sheep beta-lactoglobulin gene with two poorly expressed beta-lactoglobulin-derived hybrid genes encoding human proteins. In each case, we observed a significant improvement in the frequency and level of expression of the hybrid gene. "Rescuing" transgene expression by co-integration may provide a general solution for improving the efficiency of heterologous gene expression in transgenic animals.
D000818:Animals; D004247:DNA / Q000032:analysis; D005786:Gene Expression Regulation / Q000502:physiology*; D005818:Genetic Engineering; D007782:Lactoglobulins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008894:Milk Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis
D007782:Lactoglobulins; D008894:Milk Proteins; D012313:RNA; D004247:DNA