[Effect of D-penicillamine and 2-mercaptoethanol on human IgM in normal serum].
Serum from healthy blood donors (O Rh+) was fractionated on a Sephadex-G-200 column before and after incubation with equal volumes of either 0.2 m 2-Mercaptoethanol or D-Penicillamine. Immunoelectrophoretic analyses revealed that addition of these two sulphhydryl-containing compounds resulted in disappearance of the typical IgM-precipitate within the first peak. A new precipitate specific for M-chains appeared within the second peak. The data indicate that the smaller subunits with M-chain specificity result from splitting of disulphide bonds of the IgM molecules by the D-Penicillamine and the 2-Mercaptoethanol.
D001769:Blood / Q000187:drug effects; D004220:Disulfides; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism*; D008623:Mercaptoethanol / Q000494:pharmacology*; D010396:Penicillamine / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D013439:Sulfhydryl Reagents
D004220:Disulfides; D007075:Immunoglobulin M; D013439:Sulfhydryl Reagents; D008623:Mercaptoethanol; D010396:Penicillamine
Wirkung von D-Penicillamin und 2-Mercaptoäthanol auf humanes IgM in Normalseren