Anticodon-dependent aminoacylation of RNA minisubstrate by lysyl-tRNA synthetase.
Specific inhibition of mammalian lysyl-tRNA synthetase by polyU is shown. Inhibition of the enzyme is dependent on the length of the oligonucleotide, since oligoU molecules with a length of less than 8 residues do not inhibit the aminoacylation, whilst the effect of oligoU molecules with a length of about 30 residues is the same as that of polyU. Inhibition is a result of recognition by the enzyme of the tRNALys anticodon sequence (UUU) coded by polyU. Aminoacylation of the oligoU molecule with attached CCA sequence (G(U)20-CCA) by yeast and mammalian lysyl-tRNA synthetases is demonstrated.
D000215:Acylation; D000818:Animals; D000926:Anticodon; D007700:Kinetics; D008250:Lysine-tRNA Ligase / Q000378:metabolism*; D011072:Poly U / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D011817:Rabbits; D013379:Substrate Specificity
D000926:Anticodon; D011072:Poly U; D012313:RNA; D008250:Lysine-tRNA Ligase