Mass fragmentographic quantitation of glutamic acid and gamma-aminobutyric acid in cerebellar nuclei and sympathetic ganglia of rats.
A method for the simultaneous quantitation of glutamic acid and gamma-aminobutyric acid (GABA) in tissue by mass fragmentography has been developed. The amino and carboxylic groups of the two amino acids were in a convenient one-step reaction derivatized with pentafluoropropionic anhydride and hexafluoroisopropanol. Deuterium-labeled glutamic acid and GABA and a homologue of GABA have been used as internal standards. The usefulness of the technique has been demonstrated by measurements in parts of rat cerebellum and in the superior cervical ganglion.
D000613:Aminobutyrates / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002529:Cerebellar Nuclei / Q000032:analysis*; D005725:Ganglia, Autonomic / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D005971:Glutamates / Q000032:analysis*; D008722:Methods; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D000613:Aminobutyrates; D005971:Glutamates; D005680:gamma-Aminobutyric Acid