Modulation of voltage-activated ion currents on identified neurons of Helix pomatia L. by interleukin-1.
1. The effect of interleukin-1 (IL-1) was studied on voltage-activated ion currents of the identified central neurons of Helix pomatia L. using a two-microelectrode voltage clamp. The voltage-activated inward current (ICa) was decreased, whereas the outward current (I(net) K) was increased by IL-1. 2. IL-1 affects both the transient and the delayed rectifying potassium currents. The IL-1 modulatory effect on the voltage-activated ion currents was voltage and dose dependent. The threshold concentration for IL-1 was 2 U/ml. 3. The proposed modulatory effect of IL-1 appears to have more than one site of action on the neuron membrane ion channels. 4. Rabbit anti-human IL-1 polyclonal antiserum eliminated the IL-1 effects on the voltage-activated inward and outward currents. This is the first report demonstrating a direct effect of IL-1 modulation of voltage-activated ion currents on neurons of mollusks.
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D006372:Helix, Snails / Q000502:physiology*; D007106:Immune Sera; D007375:Interleukin-1 / Q000276:immunology / Q000494:pharmacology*; D015640:Ion Channel Gating / Q000187:drug effects*; D007473:Ion Channels / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D015221:Potassium Channels / Q000187:drug effects; D011817:Rabbits; D017472:Receptors, Interleukin-1 / Q000187:drug effects / Q000502:physiology
D015220:Calcium Channels; D007106:Immune Sera; D007375:Interleukin-1; D007473:Ion Channels; D015221:Potassium Channels; D017472:Receptors, Interleukin-1