Lens culinaris lectin immobilized on sepharose: binding and sugar-specific release of intact tissue culture cells.
Lens culinaris lectin (LCL) covalently linked to 2B Sepharose binds tissue culture cells to the matrix. This is prevented by hapten sugars specific for LCL. Unlike other immobilized lectins, lens culinaris lectin allows the removal of bound cells from the matrix on addition of the specific sugars in a concentration-dependent manner. Binding and release occur under physiological conditions. Released cells continue to grow.
D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D006241:Haptens; D037102:Lectins; D008757:Methylglucosides; D008766:Methylmannosides
D006241:Haptens; D037102:Lectins; D008757:Methylglucosides; D008766:Methylmannosides