Proteins solubilized from frog virus 3 particles: effect on transcription.
The treatment of KB cells with viral proteins solubilized from frog virus 3 particles (SVE) induced a rapid shutoff of host RNA synthesis. The RNA polymerase activities of SVE-treated cells were drastically depressed, corresonding, at least for RNA polymerase B, to a decrease in the number of enzyme molecules. In vitro, SVE had no direct effect on RNA polymerases but was capable of binding with calf thymus DNA to form an SVE-DNA complex modifying the template capacity. The effect of SVE on a transcription system consisting of cell lysates would suggest that cytoplasmic factors are necessary for expression of the inhibitory capacity of SVE.
D002277:Carcinoma; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D002474:Cell-Free System; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004794:Enzyme Repression; D006801:Humans; D009062:Mouth Neoplasms; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D012995:Solubility; D014158:Transcription, Genetic / Q000187:drug effects*; D014764:Viral Proteins / Q000494:pharmacology*
D012334:RNA, Neoplasm; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases