Uptake of 3,4-dimethoxyphenylethylamine-1-14C (14C-DMPEA) by rat tissues in vitro.
Rat heart and spleen slices were incubated with 3,4-dimethoxyphenylethylamine-1-14C(14C-DMPEA) in Krebs medium at 37 C. At the end of 5-20 min of incubation, the heart did not take up the radioactivity while the spleen did. The Km and Vmax values of uptake in the spleen were 1 x 10(-4) M and 20 nmole/g per min, respectively, and the uptake was reduced to 16.0-35.1% in the cold (4 C) and to 40.3-64.0% in Na+-free medium. Thus, the uptake was an energy-dependent active process but was only partially Na+-dependent. Spleen slices incubated with 14C-DMPEA-free medium for 15 min following incubation with 14C-DMPEA retained 41.0-74.8% of radioactivity. The uptake was insensitive to norepinephrine (0.313 and 0.939 muM), dopamine (9.98 muM), 5-hydroxytryptamine (5 muM), cocaine (14.8 muM), 1-amphetamine (0.3 and 300 muM), d-amphetamine (300 muM), and normetanephrine (45.7 muM). 6-Hydroxydopamine treatment of rats, which produced 93% reduction in the splenic norepinephrine content, did not significantly reduce uptake. Thus, the uptake of DMPEA into the spleen is not by adrenergic neurones.
D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D002250:Carbon Radioisotopes; D003042:Cocaine / Q000494:pharmacology; D003913:Dextroamphetamine / Q000494:pharmacology; D004118:Dimethoxyphenylethylamine / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D005260:Female; D006892:Hydroxydopamines / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D009647:Normetanephrine / Q000494:pharmacology; D010627:Phenethylamines / Q000378:metabolism*; D051381:Rats; D011984:Sensory Receptor Cells / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D013154:Spleen / Q000378:metabolism*; D013696:Temperature
D002250:Carbon Radioisotopes; D004118:Dimethoxyphenylethylamine; D006892:Hydroxydopamines; D010627:Phenethylamines; D009647:Normetanephrine; D012701:Serotonin; D012964:Sodium; D000661:Amphetamine; D003042:Cocaine; D003913:Dextroamphetamine; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine