Increase in galanin gene expression in locus coeruleus neurones of the rat following reserpine treatment.
Changes in galanin mRNA content in locus coeruleus neurones of the rat were studied after reserpine treatment (10 mg/kg s.c.) using an in situ hybridization technique and an alkaline phosphatase labelled oligodeoxynucleotide probe. An increase in galanin mRNA content in locus coeruleus neurones was detected as early as 3 h after reserpine treatment when compared to vehicle injected rats. A maximal increase in mRNA content was detected at 3 days after reserpine treatment. This transient increase in galanin mRNA content had subsided by post treatment day 20. The response of locus coeruleus neurones to the single reserpine injection was heterogeneous; cells in the dorsal portion of the nucleus exhibiting a greater response than ventrally located cells.
D000818:Animals; D015342:DNA Probes; D019004:Galanin; D015870:Gene Expression / Q000187:drug effects*; D017403:In Situ Hybridization; D008125:Locus Coeruleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009841:Oligonucleotides; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012110:Reserpine / Q000494:pharmacology*; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis / Q000235:genetics
D015342:DNA Probes; D009841:Oligonucleotides; D010455:Peptides; D019004:Galanin; D012110:Reserpine; D014446:Tyrosine 3-Monooxygenase