Interaction between membrane functions and protein synthesis in reticulocytes. A membrane constituent which inhibits protein synthesis in cell-free system.
The reticulocyte cell membrane was investigated for the role that it plays in cellular protein synthesis. It was found that a strong inhibition in protein synthesis occurred when a membranal extract was added to a cell-free protein-synthesizing system. The membranal extract was obtained by disrupting and then sulubilizing cell membranes with the non-ionic detergent Triton X-100. The inhibition in protein synthesis could neither be attributed to an RNAse-type action nor to a reduction in the energy level of the system. Preliminary results show that the principal site of action occurs at the elongation or/and termination stages of protein synthesis. Initial purification of the inhibitory component was achieved.
D000110:Acetylcholinesterase / Q000097:blood; D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis*; D002462:Cell Membrane / Q000378:metabolism*; D002474:Cell-Free System; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009708:Nucleotidases / Q000378:metabolism; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*
D001798:Blood Proteins; D011189:Potassium Chloride; D000110:Acetylcholinesterase; D009708:Nucleotidases; D008274:Magnesium