title: [Kinetics of enzymatic hydrolysis of quaternary aminoalkyl esters of aromatic mono- and dicarboxylic acids by butyrylcholinesterase from horse serum].
abstract: Enzymatic hydrolysis kinetics of benzoylcholine (BzCh), phehylpropionic acid choline ester (PK-157), suberic acid dicholine ester (D-6) and p-phenylenediacetic (PK-139), p-phenylenedipropionic (PK-154 and PK-155), p-phenylenediacryc (PK-150 and PK-151) and phtalic (PK-105) acids diaminoalkyl esters by horse blood serum butyrylcholinesterase (BuChE) was studied. Hydrolysis constants Km, V and Kss were estimated by means of different graphic methods. PK-157 ester turned to be highly specific selective substrate for BuChE, its V being 20 times as high and Km -- 20 times as low as those for acetylcholine (ACh). The highest V value was found for D-6 in the case of diesters. Hydrolysis of aromatic dicarbonic acids diesters was characterized with significantly lower V values (0.6-10.% of V for ACh) and extremely low Km values (approximately 10(-5) -- 10(-6) M). Substrate inhibition was observed under the hydrolysis of BzCh, PK-157, D-6 and all aromatic dicarbonic acids esters by BuChE. Formal kinetic analysis revealed that inactive complex, which formed in this case, corresponded to ES2 composition. The appearance of substrate inhibition for BuChE and its increasing are supposed to be due to the increase in the size and in the rigidity of the acyl part of the molecule in the number of substrates studied.
mesh_terms: D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood; D002264:Carboxylic Acids; D002384:Catalysis; D002802:Cholinesterases; D003998:Dicarboxylic Acids; D006736:Horses / Q000097:blood; D007700:Kinetics; D000644:Quaternary Ammonium Compounds
chemical_list: D002264:Carboxylic Acids; D003998:Dicarboxylic Acids; D000644:Quaternary Ammonium Compounds; D002091:Butyrylcholinesterase; D002802:Cholinesterases
vernacular_title: Kinetika fermentativnogo gidroliza chetvertichnykh aminoalkilovykh éfirov aromaticheskikh mono- i dikarbonovykh kislot butirilkholinesterazoĭ iz syvorotki krovi loshadi