Genomic transcriptional activity and the structure of chromatin.
Nucleic acid hybridisation has shown that micrococcal nuclease-derived chromatin subunits from the cells of Xenopus laevis contain fragments of ribosomal 28S, 18S and 5S RNAS within the population of 200 base-pair pieces of DNA. Subunits from cultured embryonic cells actively transcribing ribosomal RNA contain only 70-74% of the cistrons present in undigested wild-type DNA, while subunits from adult erythrocytes not active in RNA transcription contain close to 90% of the ribosomal cistrons in native chromatin.
D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000302:isolation & purification / Q000648:ultrastructure*; D004247:DNA / Q000302:isolation & purification; D004912:Erythrocytes; D005796:Genes; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic; D014981:Xenopus
D002843:Chromatin; D012335:RNA, Ribosomal; D004247:DNA