title: Lactic acid-utilizing bacteria in ruminal fluid of a steer adapted from hay feeding to a high-grain ration.
abstract: A mature, rumen-cannulated steer fed Coastal Bermuda grass hay for 8 weeks was adapted to a high-grain ration by stepwise increases in grain over a period of 4 weeks. The grain rations had concentrate-to-roughage ratios of 40:60, 70:30, and 85:15 and were fed for 10, 7, and 11 days, respectively. Numerical estimates of lactate-utilizing bacteria in ruminal fluid of the steer the last 3 days each ration was fed were made by colony counts. Lactate-utilizing bacteria were identified as Megasphaera elsdenii, Peptococcus asaccharolyticus, and Selenomonas ruminantium. Maximal numbers of lactate-utilizing bacteria were observed in the ruminal fluid of the steer during feeding the concentrate-to-roughage ration of 70:30.
mesh_terms: D000821:Animal Feed; D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D002417:Cattle / Q000378:metabolism / Q000382:microbiology*; D002523:Edible Grain; D006089:Gram-Negative Anaerobic Bacteria / Q000378:metabolism; D007773:Lactates / Q000378:metabolism*; D008297:Male; D010460:Peptococcus / Q000378:metabolism; D006109:Poaceae; D012417:Rumen / Q000378:metabolism / Q000382:microbiology*; D045854:Veillonellaceae / Q000378:metabolism
chemical_list: D007773:Lactates