A method for the estimation of total calcium in serum or heparinized plasma.
A simple titrimetric method for the determination of total calcium in serum or heparinized plasma is described. After removing interfering substances with an ion-exchange resin, calcium is reacted with glyoxalbis (2-hydroxyanil), GBA, and the resulting red colour titrated with ethylenediaminetetra-acetic acid in the presence of a methanol/glycerol mixture. No special equipment is used, deproteinization is not necessary and only 0.2 ml of sample is required.
D001322:Autoanalysis; D002118:Calcium / Q000097:blood*; D002852:Chromatography, Ion Exchange; D003124:Colorimetry / Q000379:methods; D005069:Evaluation Studies as Topic; D006493:Heparin; D006801:Humans; D007202:Indicators and Reagents; D010710:Phosphates / Q000097:blood; D013054:Spectrophotometry, Atomic / Q000379:methods
D007202:Indicators and Reagents; D010710:Phosphates; D006493:Heparin; D002118:Calcium