Characterization of an established cell line (SH-3) derived from pleural effusion of patient with breast cancer.
Spontaneous emergence of fast-growing cells in prolonged culture of pleural effusion cells obtained from a patient with breast cancer has led to the establishment of a cell line designated as SH-3. By morphological criteria, as revealed by light and electron microscopy, SH-3 cells are epithelial and resumble the poorly differentiated cells of a series of established human tumor cell lines. Their karyotype is hypotetraploid and different from that of HeLa cells. Isoenzyme analysis has shown the presence in the cells of a bone-type alkaline phosphatase and of the fast (A) band of G6PD. The line is free of mycoplasma.
D000469:Alkaline Phosphatase / Q000378:metabolism; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics; D002460:Cell Line; D002875:Chromosomes; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D010996:Pleural Effusion / Q000166:cytology; D011123:Polyploidy
D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase; D000469:Alkaline Phosphatase