A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.
We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012313:RNA / Q000235:genetics; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic
D012313:RNA; D004247:DNA