Hepatic DNA methylation in young, middle-aged, and senescent rats: the effect of mitogen-induced cell proliferation.
We have investigated the effect of a single dose of the hepatomitogen lead nitrate on the 5-methyldeoxycytidine (5-mdcyd) content and the HpaII, MspI, and HaeIII restriction patterns of hepatic DNA from young, middle-aged, and senescent rats. It was found that (i) the methylation pattern of genomic DNA changed significantly with age and (ii) the methylation patterns were differentially affected by the liver mitogen in the three cell populations here considered.
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D004247:DNA / Q000378:metabolism*; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008745:Methylation; D008934:Mitogens / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D008934:Mitogens; D004247:DNA; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases