title: Transitional control in Semliki forest virus infected cells.
abstract: The cistron of the 42 S RNA, which codes for other than structural proteins, is poorly translated in the middle of the growth cycle. The translational control operating in the infected cells is not expressed in cell-free protein synthesising systems programmed with the 42 S RNA, since mostly non-structural proteins are made in vitro. One of our temperature-sensitive mutants of SFV, ts-1, directs the synthesis of two non-structural proteins with molecular weights of 78,000 (ns-78) and 86,000 (ns-86) but only at the restrictive temperature. This indicates that the synthesis of the non-structural proteins is controlled by viral rather than cellular factor(s). Temperature shift experiments revealed that the control mechanism was only slowly affected by the change of the temperature.
mesh_terms: D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009154:Mutation; D010452:Peptide Biosynthesis; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism*; D012672:Semliki forest virus / Q000378:metabolism*; D013696:Temperature; D014908:Triticum / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D008715:Methionine