Evaluation of the techniques used for investigating the in vitro synthesis of bromegrass mosaic virus RNA.
The paper is dealing with the study of RNA polymerase activity using endogenous RNA templates in extracts of leaves infected with bromegrass mosaic virus (BMV). The in vitro labeling of BMV-RNA in such extracts is associated with a particulate replication complex which is able to synthesize totally or partially single-stranded BMV-RNA under conditions where exogenous BMV-RNA (purified from the virus) is rapidly degraded. This suggests that rapidly-labeled BMV-RNA is protected from endogenous nuclease effect.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003609:Dactinomycin; D003902:Detergents / Q000494:pharmacology; D001467:Hordeum / Q000378:metabolism; D009029:Mosaic Viruses / Q000378:metabolism*; D010942:Plant Viruses / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D014500:Uracil Nucleotides / Q000378:metabolism
D003902:Detergents; D012367:RNA, Viral; D014500:Uracil Nucleotides; D003609:Dactinomycin; D012321:DNA-Directed RNA Polymerases