title: Rapid screening and confirmation of amphetamine, methamphetamine, methadone, and methadone metabolite in urine by gas/thin layer chromatography.
abstract: A method suitable for large scale screening and confirmation of urine speciments for amphetamine, methamphetamine, methadone, and its primary metabolite (2-ethylidene-1,5-dimethyl-3,3-diphenlypyrrolidine) is described. The drugs are extracted from alkaline urine into an organic solvent. The amphetamine drugs are then back-extracted into a small volume of acid and identified by gas chromatography both as free bases on a 10% Apiezon L-10% KOH column and as their trifluoracetamide derivatives on a 3% OV-17 column. The organic layer, which still contains methadone and its primary metabolite, is analyzed by split-sample thin-layer chromatography using two solvent systems: ethyl acetate: methylene cloride: concentrated ammonium hydroxide (90:10:0.7) and methanol: chloroform: concentrated ammonium hydroxide (74:25:0.8). These solvent systems separate methadone from its primary metabolite without interference from other drugs or urinary substances.
mesh_terms: D000661:Amphetamine / Q000652:urine*; D001322:Autoanalysis; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D006801:Humans; D008403:Mass Screening; D008691:Methadone / Q000652:urine*; D008694:Methamphetamine / Q000652:urine*; D011759:Pyrrolidines / Q000652:urine*
chemical_list: D011759:Pyrrolidines; D008694:Methamphetamine; D000661:Amphetamine; D008691:Methadone