Induction of micronuclei in tadpoles of Rana temporaria and Xenopus laevis by the pyrethroid Fastac 10 EC.
The mutagenic properties of the pyrethroid Fastac 10 EC were estimated using the micronucleus test in tadpoles of Rana temporaria and Xenopus laevis. The frequency of erythrocytes with micronuclei was examined in blood smears obtained from animals kept for 14 days in water containing 3 different concentrations of Fastac 10 EC. The study was accompanied by a positive control using the known mutagens cyclophosphamide and N-methyl-N-nitrosourea. The results obtained showed that at high concentrations Fastac 10 EC has a clastogenic activity and/or damages the mitotic spindle, as manifested by a significant increase in the frequency of the micronucleated red blood cells. It was also demonstrated that tadpoles of Rana temporaria are more sensitive to the mutagenic effect of the pyrethroid than are those of Xenopus laevis.
D000818:Animals; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D007306:Insecticides / Q000633:toxicity*; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*; D011896:Rana temporaria; D008941:Spindle Apparatus / Q000187:drug effects; D014982:Xenopus laevis
D007306:Insecticides; D009153:Mutagens; D011722:Pyrethrins; C017160:cypermethrin