The regulation of haemoglobin synthesis in cultured chick blastoderms by steroids related to 5beta-androstane.
1. After 24h of incubation, the blastoderm may be dissected from the early developing chick embryo and successfully maintained under conditions of organ culture in vitro. 2. Low concentrations of steroids related to 5beta-androstane stimulate the synthesis of foetal haemoglobins, types E and P, in a highly steroid- and tissue-specific manner.
D000624:5-Aminolevulinate Synthetase / Q000378:metabolism; D000731:Androstanes / Q000494:pharmacology*; D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D001756:Blastoderm / Q000378:metabolism*; D002474:Cell-Free System; D002642:Chick Embryo; D004920:Erythropoiesis / Q000187:drug effects; D005043:Etiocholanolone / Q000494:pharmacology; D005319:Fetal Hemoglobin / Q000096:biosynthesis*; D009924:Organ Culture Techniques; D011061:Poly A; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D012333:RNA, Messenger; D013997:Time Factors; D014908:Triticum
D000731:Androstanes; D011506:Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D005319:Fetal Hemoglobin; D005043:Etiocholanolone; D000624:5-Aminolevulinate Synthetase