Assessment of the genotoxic potential of riboflavin and lumiflavin. B. Effect of light.
On exposure to visible light, riboflavin and lumiflavin produced reactive oxygen species such as singlet oxygen and superoxide radicals. The reaction was found to be time- and concentration-dependent. Both riboflavin and lumiflavin, upon illumination, showed mutagenic response in the umu test as well as in the Ames/Salmonella assay with Salmonella typhimurium TA102. The mutagenic response was partially abolished by superoxide dismutase while sodium azide did not have any effect. No mutagenicity was observed if the compounds were not illuminated. The results suggested the involvement of superoxide radicals in light-induced mutagenicity of riboflavin as well as lumiflavin.
D001386:Azides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005415:Flavins / Q000633:toxicity*; D008027:Light / Q000009:adverse effects*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D010100:Oxygen / Q000378:metabolism; D012256:Riboflavin / Q000633:toxicity*; D012486:Salmonella typhimurium; D019810:Sodium Azide; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors; D001616:beta-Galactosidase
D001386:Azides; D005415:Flavins; D009153:Mutagens; C001922:lumiflavin; D013481:Superoxides; D019810:Sodium Azide; D013482:Superoxide Dismutase; D001616:beta-Galactosidase; D010100:Oxygen; D012256:Riboflavin