title: Histochemical localization of nitric oxide-synthesizing neurons and vascular sites in the guinea-pig intestine.
abstract: Laminar preparations of fixed segments of the guinea-pig intestine were examined for nitric oxide synthase activity using reduced nicotinamide adenine dinucleotide phosphate and nitroblue tetrazolium salt as substrates. Under conditions specific for detecting nitric oxide synthase-related diaphorase activity, a subpopulation of neural elements in the myenteric plexus, deep muscular plexus and submucosa were intensely stained. Intensely stained nerve fibres were distributed throughout the meshworks of the myenteric plexus and its innervation of the circular muscle, and in the submucosa within Henle's plexus. Intensely stained nerve cells and their processes were evident in most myenteric ganglia but were rare in ganglia of Henle's plexus. Stained ganglion cells comprised types I, II and VI of the morphologically defined enteric nerve cells. Stained neural elements were increasingly prevalent within successively more caudal segments of the intestine. In addition to neuronal staining, arterioles of the submucosal vascular network displayed distinct, punctate patches of staining distributed over their surface. Perivascular nerve fibre staining was absent. These results show nitric oxide synthase activity to be present within neurons and fibres of the major enteric nerve layers and within submucosal blood vessels throughout the guinea-pig small and large intestine.
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000818:Animals; D006168:Guinea Pigs; D006651:Histocytochemistry; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000201:enzymology / Q000294:innervation / Q000378:metabolism*; D007422:Intestines / Q000294:innervation; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D009197:Myenteric Plexus / Q000276:immunology / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D009580:Nitroblue Tetrazolium
chemical_list: D009243:NAD; D009580:Nitroblue Tetrazolium; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases