Altered lysosomal glycohydrolase activities in juvenile diabetes mellitus.
Studies have been carried out on activities of lysosomal beta-N-acetylhexosaminidase (hex), beta-galactosidase (beta-gal), alpha-glucosidase (alpha-glu), and acid phosphatase (AP) in serum and urine from patients with juvenile diabetes and matched controls. There is a large increase in blood and urinary hex activity (the former presenting three distinct patterns of abnormality), a moderate increase in urinary beta-gal, and a small increase in urinary alpha-glu activity, but no elevation of blood or urinary AP in the diabetics. Urinary alpha-glu activity in the diabetics shows striking inhibition by glucose, and this may reflect a similar phenomenon in vivo. Although glycohydrolase activities are elevated in patients with no detectable microangiopathy, more striking changes may be observed in patients with severe small-vessel disease. These alterations may be associated with increased glycoprotein catabolism in the diabetic, an area in need of further studies in the human and experimental diabetic animal.
D000135:Acid Phosphatase / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology*; D004355:Drug Stability; D005260:Female; D005696:Galactosidases / Q000652:urine; D005947:Glucose / Q000494:pharmacology; D005959:Glucosidases / Q000652:urine; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D012737:Sex Factors
D000135:Acid Phosphatase; D005696:Galactosidases; D005959:Glucosidases; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; D005947:Glucose