The replication of rodent parvovirus X14.
The replication of rodent parvovirus X14 DNA has been studied in rat embryo tissue culture cells. Virus DNA was isolated from I M-NaCl-SDS-pronase supernatant fluids from 24 h after infection. The majority of this DNA was 1-7 mum in length and double-stranded, indicating that it was an intermediate in the replication cycle of this single-stranded DNA virus. Single-stranded DNA of equivalent length was isolated directly from X14 virions. The buoyant density of this DNA was 1-728 g/ml whereas the double-stranded form banded at 1-714 g/ml in caesium chloride gradients. Difficulties in detecting significant amounts of single-stranded viral DNA directly from infected cells would appear to indicate that progeny single-stranded DNA is rapidly encapsidated after synthesis.
D046508:Culture Techniques; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000032:analysis / Q000096:biosynthesis*; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D010321:Parvoviridae / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D014779:Virus Replication
D004277:DNA, Single-Stranded; D004279:DNA, Viral