Expression of chimeric proteins encoded by the fused BLV reverse transcriptase: beta-galactosidase in Escherichia coli.
The gene for bovine leukemia virus (BLV) reverse transcriptase was cloned in prokaryotic expression vector pUC8-2. After fusion of Escherichia coli lacZ gene to different parts of reverse transcriptase we detected expression of new proteins with molecular weights corresponding to the size of the hybrid genes. A coding region most probably responsible for about a hundred-fold decrease in expression of long fusion proteins has been identified. A few possible causes of this phenomenon were tested.
D004926:Escherichia coli; D015870:Gene Expression / Q000235:genetics; D015751:Genes, pol / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D001909:Leukemia Virus, Bovine / Q000235:genetics; D010957:Plasmids; D012194:RNA-Directed DNA Polymerase / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D012194:RNA-Directed DNA Polymerase; D001616:beta-Galactosidase