Both open reading frames of the linear plasmid pMC3-2 from the ascomycete Morchella conica are transcribed in vivo.
Mitochondrial RNA was isolated from the morel strain Morchella conica 3 harbouring the linear plasmid pMC3-2 and subjected to gel electrophoresis followed by a Northern analysis using cloned fragments of the plasmid pMC3-2 as probes. Hybridization was obtained only with central parts of pMC3-2 and specific bands of mtRNA. The hybridization bands (2.8 kb and 1.0 kb) correspond in size to the length of the two ORFs of pMC3-2 which were deduced from nucleotide-sequence data. Thus, both ORFs, one encoding a DNA polymerase and the other a yet unknown protein, are transcribed in the mitochondria of the plasmid-bearing Morchella conica strain.
D001203:Ascomycota / Q000235:genetics*; D015152:Blotting, Northern; D005800:Genes, Fungal; D016366:Open Reading Frames; D010957:Plasmids; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D000077278:RNA, Mitochondrial; D014158:Transcription, Genetic
D012331:RNA, Fungal; D000077278:RNA, Mitochondrial; D012313:RNA