Carbohydrate-binding properties of an immobilized alpha-D-galactopyranosyl-binding protein (lectin) from the seeds of Bandeiraea simplicifolia.
The alpha-D-galactopyranosyl-binding lectin from Bandeiraea simplicifolia has been coupled to cyanogen bromide-activated Sepharose 4B. Using this immobilized system, we have been able to study the interaction of the lectin with model carbohydrate-protein conjugates and polysaccharides, and to reaffirm this protein's carbohydrate-binding specificity. The opportunity for the isolation of biopolymers containing alpha-D-galactopyranosyl end-groups is demonstrated by the single-step purification of a new galactomannan from the seeds of Cassia alata.
D001665:Binding Sites; D002846:Chromatography, Affinity; D005690:Galactose; D037102:Lectins; D037121:Plant Lectins; D011485:Protein Binding; D012639:Seeds / Q000032:analysis*
D037102:Lectins; D037121:Plant Lectins; D005690:Galactose