title: Quantitation of glycine in plasma and urine by chemical ionization mass fragmentography.
abstract: A new technique has been developed for rapid and precise quantitation of glycine in micro samples of biological fluids. Glycine is measured as the n-butyl N-trifluoroacetyl (TAB) derivative by chemical ionization mass fragmentography, using both methane and isobutane as carrier/reagent gases, with the analogous derivative of 2,2-dideuterioglycine added as the internal standard. This technique is also readily applicable, with sensitivity to the femtomole level, to determinations of other amino acids, as, for example, in studies of congenital errors of metabolism.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005998:Glycine / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans
chemical_list: D000596:Amino Acids; D005998:Glycine