Establishment and characterization of two new cell lines derived from squamous cell carcinoma of the tongue in Chinese patients.
Two new cell lines derived from squamous cell carcinoma of the tongue, T1/CUHK and T2/CUHK, have been established in culture. Analysis of the morphology, ultrastructure, chromosome number, spheroid formation and immunohistochemical properties of the two cell lines demonstrated that they are both well characterized. T1/CUHK cells grew relatively faster than T2/CUHK cells. Both cell lines were tumorigenic after inoculation into made mice and showed positive reactivity with HPV 16 DNA probe. The reactivity of both cell lines with HPV 18 DNA probe was weak.
D000818:Animals; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000601:surgery; D002460:Cell Line; D002681:China / Q000208:ethnology; D002877:Chromosomes, Human; D046508:Culture Techniques / Q000379:methods; D015342:DNA Probes; D006723:Hong Kong; D006801:Humans; D007633:Keratins / Q000032:analysis; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D014062:Tongue Neoplasms / Q000473:pathology* / Q000601:surgery; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D007633:Keratins