[Effect of heparin on oxidative phosphorylation and ultrastructure of rabbit heart mitochondria].
The effect of heparin on isolated rabbit heart mitochondria was studied in usual clinical (0.25 u/ml) and higher doses. Hepain concentration 0.25 u/ml stimulated respiration in the state 4, decreased ADP/O ratio and phosphorylation rate and did not affect mitochondria ultrastructure. Higher concentration caused two-phase changes in respiration-short-time stimulation with the following inhibition, oxidative phosphorylation (respiratory control, ADP/O, phosphorylation rate) was progressively depressed and decoupled. The mitochondria ultrastructure is changed under the influence of 1.25 u/ml and of higher doses; the external membrane lost its two-layer character, the crista were converted into a small granular mass. Concentration of 1.25 u/ml transformed many mitochondria into empty vesicles.
D000818:Animals; D008854:Microscopy, Electron; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009206:Myocardium / Q000166:cytology; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D011817:Rabbits
Vpliv heparinu na okisniuval'ne fosforiliuvannia ta ul'trastruktury mitokhondriĭ sertsia krolia