title: Chiral recognition of prochiral centres and general acid-base catalysis. Necessarily interrelated manifestations of active-site structure.
abstract: On the basis of Ogston's [(1948) Nature (London) 162, 963] argument, the following conclusions are indicated by the stereochemistry of the reversible oxidation of glycollate by lactate dehydrogenase: (1) general acid-base catalysis is involved in the reaction; (2) the transformation of enzyme-bound (tetrahedral) substrate into enzyme-bound (trigonal) product involves a conformational transition of the enzyme-coenzyme complex.
mesh_terms: D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D006016:Glycolates / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*
chemical_list: D006016:Glycolates; D006038:Glyoxylates; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase