Micromonospora-produced gentamicin components.
After the chromatographic separation of [methyl-(14)C]gentamicin major (C) components from a large-scale radioactive fermentation (Lee et al., 1974), [methyl-(14)C]gentamicin minor (polar) components (A, B, B(1), X(2), and G-418) were isolated from subsequent chromatography of the remaining antibiotic mixture. When l-[methyl-(14)C]methionine was added at the onset of biosynthesis of the gentamicin components, incorporation of label into the minor components preceded incorporation into the major components. Degradation occurred when [methyl-(14)C]gentamicin major components (C(1), C(2) and C(1)a) were added respectively to the gentamicin-producing culture medium and shaken.
D001673:Biodegradation, Environmental; D003470:Culture Media; D005839:Gentamicins / Q000096:biosynthesis*; D008715:Methionine / Q000378:metabolism; D008847:Micromonospora / Q000378:metabolism*
D003470:Culture Media; D005839:Gentamicins; D008715:Methionine