Identification of proteins of the kallikrein family by isoelectrofocusing and immunoblotting.
We have found that kallikrein-like proteins differ in their isoelectric point but share antigenic determinants. For identification of kallikrein-like proteins an initial separation was carried out in flat-bed isoelectrofocusing gels. The kallikrein-like nature was demonstrated by an immunological similarity to kallikrein-like proteins by immunoblotting using antiserum against a kallikrein family member for staining. We used this system to identify different kallikrein-like proteins during purification of both known as well as new enzymes.
D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes / Q000302:isolation & purification; D015151:Immunoblotting; D007525:Isoelectric Focusing / Q000379:methods; D007526:Isoelectric Point; D007610:Kallikreins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008297:Male; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D051381:Rats; D017208:Rats, Wistar; D013363:Submandibular Gland / Q000737:chemistry
D000939:Epitopes; D011506:Proteins; D007610:Kallikreins