title: The electrophoretic mobility of double-stranded RNA in polyacrylamide gels as a function of molecular weight.
abstract: A re-evaluation of the mobility of double-stranded RNA on polyacrylamide gels over a molecular weight range of 0.46-6.3 . 10(6) was carried out using double-stranded RNAs of: bacteriophage ø6; virus like particles or mycoviruses of Penicillium chyrsogenum, Penicillium stoloniferum and Helminthosporium maydis, and reovirus type III. When the relative mobility on polyacrylamide gels was plotted as a function of log molecular weight, a smooth curve could be drawn which passed through all points. The implications of these findings to the determination of molecular weight of double-stranded RNA by polyacrylamide gel electrophoresis are discussed.
mesh_terms: D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000302:isolation & purification; D013045:Species Specificity
chemical_list: D012367:RNA, Viral