Rhodamine-labelled phalloidin stains components in the chromosomal spindle fibres of crane-fly spermatocytes and Haemanthus endosperm cells.
In crane-fly spermatocytes and Haemanthus endosperm, all metaphase and anaphase chromosomal spindle fibres were stained with rhodamine-labelled phalloidin. In crane-fly spermatocytes, each kinetochore was stained with rhodamine-labelled phalloidin at diakinesis of prophase and after colcemid caused metaphase spindles to depolymerize. Since phalloidin stains actin filaments, the distributions of rhodamine-labelled phalloidin-stained material in crane-fly spermatocytes and Haemanthus endosperm suggest that actin filaments might interact with microtubules to produce forces that move chromosomes during cell division, either directly or via an intermediate motor molecule.
D000818:Animals; D002453:Cell Cycle; D003703:Demecolcine / Q000494:pharmacology; D004175:Diptera / Q000166:cytology*; D007211:Indoles; D008297:Male; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure; D010590:Phalloidine; D010944:Plants / Q000648:ultrastructure; D012235:Rhodamines; D012617:Sea Urchins / Q000196:embryology*; D012639:Seeds / Q000648:ultrastructure*; D013090:Spermatocytes / Q000648:ultrastructure*; D008941:Spindle Apparatus / Q000187:drug effects / Q000648:ultrastructure*; D013194:Staining and Labeling; D014404:Tubulin / Q000032:analysis; D015053:Zygote / Q000648:ultrastructure*
D007211:Indoles; D012235:Rhodamines; D014404:Tubulin; D010590:Phalloidine; C007293:DAPI; D003703:Demecolcine