title: The storage of cow eggs at room temperature and at low temperatures.
abstract: The survival and development of cow eggs in the rabbit oviduct after storage at room temperature and after cooling and storage at 0-7-5 degrees C was examined. In PBS medium at room temperature 88% of Day-5 and 85% of Day-3 eggs showed normal development, but in TCM 199, 71% of Day-5 and only 49% of Day-3 eggs showed normal development. Duration of storage (1 1/2-2 hr or 6 1/2-7 1/2 hr) and cleavage stage before storage had no appreciable effect on development. Some retardation of development occurred in Day-3 eggs after 96 hr in the rabbit oviduct when compared to Day-5 eggs after 48 hr. Cooling of Day-5 and Day-6 eggs to 0-7-5 degrees C resulted in degeneration of a large proportion of eggs. Of the factors examined, storage medium (PBS or PBS+20%FCS), storage time (2 min, 24 hr) and storage temperature (0, 2, 5 or 7-5 degrees C) had little effect, but slower cooling rates tended to improve survival of eggs although the differences were not significant. More morulae (greater than 32 cells) than 8-to 24-celled eggs developed normally.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000648:ultrastructure; D002417:Cattle; D003080:Cold Temperature / Q000009:adverse effects; D003470:Culture Media; D005260:Female; D010063:Ovum / Q000648:ultrastructure; D011247:Pregnancy; D011817:Rabbits; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation; D014183:Transplantation, Heterologous; D015053:Zygote / Q000637:transplantation*
chemical_list: D003470:Culture Media