title: Induction of haemolytic anaemia by substituted phenylhydrazines.
abstract: The induction of anaemia and reticulocytosis by arylhydrazines was influenced by substituents on the benzene ring of phenylhydrazine. Arylhydrazines with ortho substituents, which would hinder the binding by haemoglobin of a ligand derived from the arylhydrazine, resulted in the least anaemia and reticulocytosis. These results are consistent with a previous finding that hydrazinobenzoic acid, which did not produce a ferrihaemochrome from ferrihaemoglobin, did not induce anaemia. The parallelism between the formation of a ferrihaemoglobin compound and the induction of anaemia supports the hypothesis that destabilization of haemoglobin by the binding of a ligand derived from the aryl portion of an arylhydrazine is an essential step in arylhydrazine-induced haemolytic anaemia.
mesh_terms: D000743:Anemia, Hemolytic / Q000097:blood / Q000139:chemically induced*; D000818:Animals; D001772:Blood Cell Count; D004906:Erythrocyte Count; D005260:Female; D006366:Heinz Bodies / Q000378:metabolism; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D010659:Phenylhydrazines / Q000633:toxicity*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D013997:Time Factors
chemical_list: D006454:Hemoglobins; D010659:Phenylhydrazines