Permeation of cyclophosphamide (NSC-26271) metabolites into tumor cells.
The permeation kinetics of cyclophosphamide and its metabolites were studied with Ehrlich ascites tumor cells, murine L1210 leukemia cells, and mouse L929 fibroblasts at 1 degrees C. In contrast to carboxyphosphamide and nor-nitrogen mustard, an equipartition of cyclophosphamide and 4-hydroperoxycyclophosphamide was observed in these cells. First experiments have been done to study the efflux of cyclophosphamide and 4-hydroperoxycyclophosphamide after incubation until saturation with Ehrlich ascites tumor cells. Cyclophosphamide could be washed out completely, whereas, 4-hydroperoxyclophosphamide shows an apparent retention in the cell at 37 degrees C.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000378:metabolism*; D007700:Kinetics; D007739:L Cells / Q000378:metabolism; D007939:Leukemia L1210 / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism*; D009588:Nitrogen Mustard Compounds / Q000378:metabolism
D009588:Nitrogen Mustard Compounds; D003520:Cyclophosphamide