Endogenous aFGF expression and cellular changes after a demyelinating lesion in the spinal cord of adult normal mice: immunohistochemical study.
Fibroblast growth factors (FGFs) are known to act on glial cells in vitro. At the present time, their involvement in the remyelinating process of the adult central nervous system (CNS) is still unknown. In the present study, using immunohistochemistry (IHC), we investigated the evolution in time and space of acidic FGF (aFGF) expression and CNS cell changes occurring after a chemically induced demyelinating lesion. In a first early period, aFGF immunostaining was shown to decrease around the demyelinated area. A dramatic increase was then observed and was accompanied by an increase of cell density around and inside the lesion. This was correlated with the beginning of remyelination. Late after demyelination, while remyelination was still in progress, aFGF immunostaining of the lesion and unlesioned spinal cord were comparable. A role of aFGF in remyelination is proposed.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D003711:Demyelinating Diseases / Q000378:metabolism / Q000473:pathology*; D016220:Fibroblast Growth Factor 1 / Q000096:biosynthesis*; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D013118:Spinal Cord Diseases / Q000378:metabolism / Q000473:pathology*
D000906:Antibodies; D005904:Glial Fibrillary Acidic Protein; D016220:Fibroblast Growth Factor 1