[EBV in malignant lymphomas].
PCR and in-situ-Hybridization were used to detect EBV-DNA within Hodgkin- and Non-Hodgkin-Lymphomas. By the use of primers for the Bam H1 W fragment we could show EBV-DNA to be associated with 45% Hodgkin- and 25% T-Lymphomas, whereas we could not find EBV-DNA within B-Lymphomas. In about one third of the PCR-positive cases we could localize EBV-DNA mostly in the tumor cell nuclei by in-situ-hybridization.
D001483:Base Sequence; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000382:microbiology / Q000473:pathology; D006801:Humans; D017403:In Situ Hybridization; D008223:Lymphoma / Q000382:microbiology* / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000382:microbiology / Q000473:pathology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods
D015345:Oligonucleotide Probes
EBV bei malignen Lymphomen.