Pregnanetriolone, a normal steroid metabolite: its excretion by normal, Cushing's syndrome and congenital adrenal hyperplasia subjects.
Synthesis of 3H-pregnanetriolone permitted the estimation of pregnanetriolone in urine with a sensitivity in excess of most previous claims. A good correlation (r = +0.97) was obtained between the values from gas liquid chromatography and those of a double isotope derivative method. In contrast to previous reports, these methods indicated that pregnanetriolone is excreted by normal adults. Urinary pregnanetriolone levels were 18-59 mug/24hr for normal subjects, 35-290mug/24hr in Cushing's syndrome and 250-7000 mug/24hr with congenital adrenal hyperplasia. It is concluded that pregnanetriolone is a normal steroid metabolite and its occurrence in Cushing's syndrome does not necessary indicate an abnormal steroid biosynthetic pathway.
D000307:Adrenal Gland Diseases / Q000652:urine*; D000328:Adult; D002845:Chromatography; D002849:Chromatography, Gas; D003460:Crystallization; D003480:Cushing Syndrome / Q000652:urine*; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000652:urine; D007223:Infant; D007664:Ketosteroids / Q000652:urine; D008297:Male; D011279:Pregnanetriol / Q000031:analogs & derivatives* / Q000652:urine
D007664:Ketosteroids; D011279:Pregnanetriol; C009030:pregnanetriolone