Quantitative determination of N-terminal amino acids of peptides and proteins with cobalt(III) chelates.
Quantitative N-terminal peptide-bond hydrolysis with the cis-beta-hydroxyaquo(triethylenetetramine) cobal (III) ion, i.e. beta-[Co(trien)(OH)(OH2)]2+, is reported. The method has been demonstrated with 20 small peptides, a hexapeptide, bradykinin, insulin A chain (oxidized), glucagon and insulin. The procedure involves no acidic hydrolysis step and thus no destruction of labile amino acids.
D000596:Amino Acids / Q000032:analysis*; D002614:Chelating Agents; D003035:Cobalt; D010455:Peptides / Q000032:analysis*
D000596:Amino Acids; D002614:Chelating Agents; D010455:Peptides; D003035:Cobalt