Anti-caldesmon monoclonal antibody reverses the inhibition of actomyosin Mg(2+)-ATPase activity by caldesmon.
Five mouse monoclonal antibodies, CaD 1-5, against chicken gizzard caldesmon were prepared. One of them (CaD4) was characterized by means of immunoblotting and its effect on actomyosin Mg(2+)-ATPase activity. CaD4 recognized the tropomyosin-binding site of caldesmon. CaD4 reversed the caldesmon-induced inhibition of actomyosin Mg(2+)-ATPase activity in a dose-dependent manner. These results suggest that the epitope recognized by CaD4 is an important domain for the function of caldesmon on the actinmyosin interaction in the smooth muscle contraction-relaxation system.
D000205:Actomyosin / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001665:Binding Sites; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D014335:Tropomyosin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D002148:Calmodulin-Binding Proteins; D000939:Epitopes; D014335:Tropomyosin; D000205:Actomyosin; D017301:Ca(2+) Mg(2+)-ATPase