[Escherichia coli ribosomes having peptidyl-tRNA and deacylated tRNA at the A- and P-sites, respectively, may not be competent in translocation].
Ribosomes can have two states at 0 degree C: competent and noncompetent in translocation. In both states poly(U)-programmed ribosomes bind phenylalanyl-tRNA to A and P sites and form peptide bond. Elongation factor G promotes fast translocation in competent ribosomes and makes them noncompetent ones. Initial correlation between competent and noncompetent ribosomes is 2:1. Addition of deacylated tRNA does not influence phenomenon described as well as thermal reactivation of the ribosomes before beginning of the experiments. The possibility of deacylated tRNA translocation is shown. The translocation does not occurred provided that at least one of the ribosome sites is filled with shortened tRNA analog (tRNA with truncated CCA-end or tRNA anticodon arm).
D000215:Acylation; D004926:Escherichia coli / Q000235:genetics*; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors / Q000502:physiology; D010455:Peptides / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D012360:RNA, Transfer, Phe / Q000235:genetics; D012270:Ribosomes / Q000502:physiology*; D014178:Translocation, Genetic / Q000235:genetics*
D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D010455:Peptides; D012329:RNA, Bacterial; D012360:RNA, Transfer, Phe; D012343:RNA, Transfer
Rybosomy Escherichia coli, shcho maiut' u A- ta P-saĭtakh vidpovidn'o peptydyl-tRNK ta deatsyl'ovanu tRNK, mozhut' buty nekompetentni u transl'okatsiï.