Structure and composition of intracytoplasmic membranes of Ectothiorhodospira mobilis.
The lamellar membrane stacks of Ectothiorhodospira mobilis were isolated and purified by a combination of lysozyme and osmotic shock treatment, followed by differential and density gradient centrifugation. Preparations of lamellar membranes were enriched at least 2.4-fold in the ratio of bacteriochlorophyll a to protein. Thin-sectioning, negative staining, platinum-carbon shadowing and freeze-etching were used to study the architecture of the membrane units. Both platinum-carbon shadowing and freeze-etching showed the outer-surfaces of the isolated lamellar membrane stacks to be relatively smooth. Particles averaging 7 nm in diameter were seen on several faces following freeze-etching.
D000596:Amino Acids / Q000032:analysis; D002458:Cell Fractionation / Q000379:methods; D003521:Cyclopropanes / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D010788:Photosynthesis; D012245:Rhodospirillales / Q000648:ultrastructure*
D000596:Amino Acids; D003521:Cyclopropanes; D005227:Fatty Acids