[Chromatographic analysis of urinary amino acids in Paget's disease. I : Biochemical study. Isolation and amino acid composition of a urinary peptide specific to this bone disease].
The authors demonstrated in the urine of patients presenting Paget's bone disease a peptide rich in hydroxyproline. The level of this compound expressed as "norleucine equivalent" was determined by chromatographic analysis of the urinary amino acids. There was a very good correlation between the total hydroxyproline level and the quantity of this peptide in the urine. The authors isolated this peptide and determined that it contained 3 hydroxyprolines for every 2 glutamines. This peptide thus seems to indicate an anomaly in the chain of synthesis; a disorder of the collagen metabolism would produce in these patients large quantities of these molecules that are excreted via the kidney. At the present stage of the author's studies this urinary peptide appears to be "specific" to Pagets disease.
D000596:Amino Acids / Q000032:analysis; D001322:Autoanalysis; D003094:Collagen / Q000378:metabolism; D005971:Glutamates / Q000652:urine; D006801:Humans; D006909:Hydroxyproline / Q000652:urine*; D010001:Osteitis Deformans / Q000378:metabolism / Q000652:urine*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000302:isolation & purification / Q000652:urine*
D000596:Amino Acids; D005971:Glutamates; D010455:Peptides; D003094:Collagen; D006909:Hydroxyproline
Analyse chromatographique des acides aminés urinaires au cours de la maladie de Paget. Partie I : étude biochimique. Isolement et composition en acides aminés d'un peptide urinaire "spécifique" de cette atteinte osseuse