Influence of glucocorticoid, estrogen, and androgen hormones on transformation of human cells in vitro by feline sarcoma virus.
Infection of human foreskin cells (D-550) by the Snyder-Theilen strain of feline sarcoma virus produced small but countable foci and demonstrated "single-hit" dose-response kinetics. Significant quantitative and qualitative enhancement of focus formation was observed when the glucocorticoid hormones, dexamethasone, hydrocortisone, cortisol acetate, and prednisolone were added to cell cultures (1.0 mug/ml) 24 hr postinfection. However, aldosterone, while inducing qualitatively larger foci, did not bring about a quantitative enhancement in total foci number. By contrast, 17beta-estradiol, progesterone, cortisone acetate, methyltestosterone, and estrone elicited little or no effect on focus induction by Snyder-Theilen feline sarcoma virus. Evidence is suggestive of a posttranscriptional effect possibly modulating viral genome expression resulting in an increased efficiency of viral transformation, and an increased proliferation of transformed cells.
D000450:Aldosterone / Q000494:pharmacology; D000728:Androgens / Q000494:pharmacology*; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D002999:Clone Cells; D003348:Cortisone / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D004970:Estrone / Q000494:pharmacology; D005938:Glucocorticoids / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D008777:Methyltestosterone / Q000494:pharmacology; D009858:Oncogenic Viruses; D011239:Prednisolone / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology; D012509:Sarcoma / Q000382:microbiology
D000728:Androgens; D004967:Estrogens; D005938:Glucocorticoids; D004970:Estrone; D000450:Aldosterone; D011374:Progesterone; D004958:Estradiol; D003907:Dexamethasone; D011239:Prednisolone; D003348:Cortisone; D008777:Methyltestosterone; D006854:Hydrocortisone