title: Molecular sieving by glomerular basement membrane isolated from normal and nephrotic rabbits.
abstract: Analog chromatography with glomerular basement membrane isolated from normal and nephrotic rabbits was performed to see if there was a molecular sieving effect by the basement membrane. In a normal glomerular basement membrane column, 125I-labeled human serum globulin and 131I-labeled human serum albumin were eluted at the same position, probably at the void volume of the column. However, 125I-labeled insulin and tritiated water appeared with a delay and gave separated peaks. In nephrotic glomerular basement membrane, 125I-labeled human globulin, 131I-labeled serum albumin, 125I-labeled insulin and tritiated water gave four distinctly separated peaks in that order. By analogy with Sephadex, the sieving effect of glomerular basement membrane was demonstrated.
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism*; D007328:Insulin; D007672:Kidney Cortex / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000378:metabolism*; D008970:Molecular Weight; D009401:Nephrosis / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D012709:Serum Albumin; D012712:Serum Globulins
chemical_list: D007328:Insulin; D011506:Proteins; D012709:Serum Albumin; D012712:Serum Globulins