title: Platelet-derived growth factor and growth-related genes in rat lung. II. Effect of exposure to 85% O2.
abstract: The expression of platelet-derived growth factor (PDGF), its receptor, and related genes was studied in the lung tissue of rats exposed to air or 85% O2. PDGF-B chain mRNA was increased by 6 days and PDGF B-type receptor mRNA was increased by 4 and 6 days of exposure to 85% O2. Despite a continued increase of cell division, both PDGF-B chain and B-type receptor mRNAs had returned to control values by 14 days of exposure to 85% O2. PDGF-A chain mRNA was unaffected by exposure to 85% O2. Nuclear runoff transcription analysis confirmed increased transcription of PDGF-B chain mRNA, whereas Western blot analysis of lung homogenates suggested consequent increased translation of PDGF-B chain mRNA to PDGF-BB at 7 days of exposure to 85% O2. Combined immunocytochemistry and autoradiography localized PDGF-BB to the major site of cell division, the pulmonary interstitium. We speculate that the early pulmonary fibroblast hyperplasia observed following exposure to 85% O2 is mediated by increased PDGF-B chain gene expression and may also be mediated by changes in PDGF B-type receptor gene expression.
authors.affiliation: Department of Paediatrics, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada.
authors.forename: R N
authors.initials: RN
authors.lastname: Han
authors.forename: S
authors.initials: S
authors.lastname: Buch
authors.forename: B A
authors.initials: BA
authors.lastname: Freeman
authors.forename: M
authors.initials: M
authors.lastname: Post
authors.forename: A K
authors.initials: AK
authors.lastname: Tanswell
mesh_terms: D000818:Animals; D001345:Autoradiography; D005260:Female; D005796:Genes; D007150:Immunohistochemistry; D008168:Lung / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D013936:Thymidine / Q000493:pharmacokinetics
chemical_list: D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor; D010100:Oxygen; D013936:Thymidine