title: [Hemolysin from Vibrio cholerae eltor: cloning and expression of genes in Escherichia coli].
abstract: A 6.56-kb V. cholerae eltor DNA fragment encoding hemolysin synthesis was cloned in pUC18. The resultant recombinant plasmid pES4H (9.25 kb) was mapped by restriction analysis and shown to express in different E. coli strains as well as in nonhemolytic V. cholerae strains. Application of the cloned fragment as a molecular probe revealed homologous sequences in all V. cholerae strains tested independently on their biotypes, hemolytic activity and presence of vct-genes in their genomes while none of other Vibrio species and related microorganisms contained such sequences. A recombinant E. coli strain, a V. cholerae eltor hemolysin producer, was constructed. The simultaneous expression of hemolytic and toxinogenic properties by the same V. cholerae strains is discussed.
authors.forename: V P
authors.initials: VP
authors.lastname: Vlasov
authors.forename: E V
authors.initials: EV
authors.lastname: Monakhova
authors.forename: I E
authors.initials: IE
authors.lastname: Ushakova
authors.forename: M L
authors.initials: ML
authors.lastname: Mikhas'
authors.forename: E B
authors.initials: EB
authors.lastname: Danilkina
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Lomov
mesh_terms: D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli; D015870:Gene Expression; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics*; D010957:Plasmids; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D006460:Hemolysin Proteins
vernacular_title: Gemolizin Vibrio cholerae eltor: klonirovanie i ékspressiia genov v Escherichia coli.