title: Molecular cloning of the acid sphingomyelinase of the mouse and the organization and complete nucleotide sequence of the gene.
abstract: The cDNA and complete gene encoding mouse acid sphingomyelinase (ASM) has been isolated using a homology screening approach. Comparison with the human sequence shows 81% sequence identity at cDNA level and 82% at the protein level. Homology is markedly reduced in the N-terminal region, especially in the presumed signal peptide. The six-exon gene structure is similar to the human one, except for the position of Alu1 elements. The alternatively used splice site 40 bp downstream of the human exon 2 is not conserved in the mouse gene and accordingly no such alternative splicing was found in mouse.
authors.affiliation: Institut für Biochemie, Köln.
authors.forename: D
authors.initials: D
authors.lastname: Newrzella
authors.forename: W
authors.initials: W
authors.lastname: Stoffel
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D017354:Point Mutation; D013108:Sphingomyelin Phosphodiesterase / Q000235:genetics*
chemical_list: D009841:Oligonucleotides; D004247:DNA; D013108:Sphingomyelin Phosphodiesterase