Mutations in both the 2B and 2C genes of hepatitis A virus are involved in adaptation to growth in cell culture.
Oligonucleotide-directed mutagenesis of an infectious cDNA clone of wild-type hepatitis A virus was performed to determine which mutations acquired in the nonstructural 2B and 2C genes during adaptation to growth in cell culture were effective in enhancing virus growth in vitro. Results of transfection assays demonstrated that one mutation in the 2B gene and two mutations in the 2C gene were responsible for an increased efficiency in growth, but growth enhancement required the participation of at least two of the three mutations.
Emerson
S U
SU
Hepatitis Viruses Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Huang
Y K
YK
McRill
C
C
Lewis
M
M
Purcell
R H
RH
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D006507:Hepatovirus / Q000235:genetics* / Q000254:growth & development; D006801:Humans; D016297:Mutagenesis, Site-Directed; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection
D003062:Codon; D004279:DNA, Viral