title: Use of a different buffer system in the phenolic glycolipid-I ELISA.
abstract: By changing the buffer system in the phenolic glycolipid-I (PGL-I) enzyme-linked immunosorbent assay (ELISA) the sensitivity of the test was increased without altering its specificity. Using a Tris-HCl buffer, significant titers of > or = 1:300 were found in 53.1% of the sera in paucibacillary (PB) and 98.0% of the sera in multibacillary (MB) groups of patients. Titer levels were also significantly increased. In the PB group of patients with Tris-HCl, the highest titer detected was 1:1200; in the MB group of patients, 1:76,800. Through this modification of the buffer system a more sensitive test was obtained thereby increasing the detectable level of PGL-I antibodies in both the PB and MB groups of patients.
authors.affiliation: Armauer Hansen Institute/DAHW, Wurzburg, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Sticht-Groh
authors.forename: A E
authors.initials: AE
authors.lastname: Alvarenga
authors.forename: L
authors.initials: L
authors.lastname: Vettom
authors.forename: W
authors.initials: W
authors.lastname: von Ballestrem
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology; D002021:Buffers; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007918:Leprosy / Q000276:immunology; D008193:Lyme Disease / Q000276:immunology; D009166:Mycobacterium leprae / Q000276:immunology*; D012680:Sensitivity and Specificity; D014325:Tromethamine
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D002021:Buffers; D006017:Glycolipids; D007074:Immunoglobulin G; C045169:phenolic glycolipid I, Mycobacterium leprae; D014325:Tromethamine