Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants.
Embryogenic maize (Zea mays L.) callus cultures were transformed by microprojectile bombardment with a chimeric hygromycin phosphotransferase (HPT) gene and three transformed lines were obtained by selecting for hygromycin resistance. All lines contained one or a few copies of the intact HPT coding sequence. Fertile, transgenic plants were regenerated and the transmission of the chimeric gene was demonstrated through two complete generations. One line inherited the gene in the manner expected for a single, dominant locus, whereas two did not.
Walters
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Plant Science Research, Inc., Minnetonka, MN 55343.
Vetsch
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Potts
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Lundquist
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D001483:Base Sequence; D015139:Blotting, Southern; D002934:Cinnamates; D046508:Culture Techniques; D004351:Drug Resistance / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor); D030821:Plants, Genetically Modified / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014170:Transformation, Genetic; D003313:Zea mays / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
D002934:Cinnamates; D005819:Genetic Markers; D011993:Recombinant Fusion Proteins; D006921:Hygromycin B; C026273:hygromycin A; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase