Effect of dibutyryl cyclic adenosine 3',5'-monophosphate on morphological features and biological markers of human salivary myoepithelial cell line in culture.
We have found the emergence of myoepithelial cells (HSG-AZA1) in neoplastic human salivary intercalated duct cell line HSG in culture after treatment with 5-azacytidine. When HSG-ZAZ1 cells were cultured in the presence of N6,O2-dibutyryl cyclic adenosine 3',5'-monophosphate (dB-cAMP), they formed long cytoplasmic processes which were densely packed with ample microfibrils in addition to microtubule bundles. The expression of neuron-specific enolase, synaptophysin, and catecholamine as well as neurofilaments in the treated HSG-AZA1 cells was found by the immunofluorescence staining technique, immunoblotting, immunoelectron microscopy, or catecholamine fluorescence. Both the anchorage-independent and anchorage-dependent growths of HSG-AZA1 cells were suppressed in the presence of dB-cAMP. After the removal of dB-cAMP from the culture, the treated cells returned rapidly to the phenotype and growth rate of the untreated cells. These findings indicate that reversible differentiation into the neuron-like cells of HSG-AZA1 cells occurs in growth medium containing dB-cAMP.
Kawamata
H
H
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Japan.
Azuma
M
M
Yanagawa
T
T
Yura
Y
Y
Yoshida
H
H
Furumoto
N
N
Sato
M
M
D000704:Analysis of Variance; D003994:Bucladesine / Q000494:pharmacology*; D002395:Catecholamines / Q000378:metabolism*; D002460:Cell Line; D002999:Clone Cells; D000242:Cyclic AMP / Q000378:metabolism*; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006801:Humans; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D012468:Salivary Gland Neoplasms; D016708:Synaptophysin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D002395:Catecholamines; D016708:Synaptophysin; D003994:Bucladesine; D000242:Cyclic AMP; D010751:Phosphopyruvate Hydratase