title: [Preservation of Pseudomonas putida bacteria at low temperatures].
abstract: We have found that the mode of cooling, composition of cryopreservation medium, original concentration of cells and storage temperature affect viability of Pseudomonas putida bacteria during low-temperature preservation. We have elaborated the conditions of preservation, providing for a high survival of bacteria, namely: one-stage cooling with the rates of 30, 40 degrees C/min or immersion into liquid nitrogen in the culturing medium with addition of sucrose, glycerol or dimexide in the concentration of 0.5 M; storage temperature is -80 degrees C divided by -196 degrees C.
authors.forename: I P
authors.initials: IP
authors.lastname: Vysekantsev
authors.forename: T K
authors.initials: TK
authors.lastname: Krasheninnikova
authors.forename: E V
authors.initials: EV
authors.lastname: Olekhnovich
authors.forename: L V
authors.initials: LV
authors.lastname: Stepaniuk
mesh_terms: D015925:Cryopreservation; D016958:Pseudomonas putida
vernacular_title: Konservirovanie bakteriĭ Pseudomonas putida pri nizkikh temperaturakh.