Polysaccharide-coated liposomal amphotericin B for the treatment of murine pulmonary candidiasis.
Amylopectin-coated liposomal amphotericin B was investigated in a murine model of pulmonary candidiasis. The LD50 of amylopectin-coated liposomal amphotericin B in normal mice was more than 10.0 mg/kg, and that of conventional amphotericin B was 1.2 mg/kg. Amylopectin-coated liposomes showed twice the concentration in the lungs of conventional liposomes. Candida albicans was inoculated intratracheally into BALB/C mice. Twenty-four hours later, the number of Candida in the lungs of mice treated with amylopectin-coated liposomes was less than in those treated with conventional liposomes, and amylopectin-coated liposomes improved the survival rate of inoculated mice. Coating liposomes with amylopectin aids the targeting of amphotericin B to the lungs.
Miyazaki
T
T
Second Department of Internal Medicine, Nagasaki University School of Medicine.
Kohno
S
S
Sasayama
K
K
Inoue
Y
Y
Hara
K
K
Ogasawara
M
M
Sato
T
T
Sunamoto
J
J
D000666:Amphotericin B / Q000493:pharmacokinetics / Q000627:therapeutic use* / Q000633:toxicity; D000687:Amylopectin / Q000493:pharmacokinetics / Q000627:therapeutic use* / Q000633:toxicity; D000818:Animals; D002176:Candida albicans / Q000254:growth & development; D002177:Candidiasis / Q000188:drug therapy* / Q000382:microbiology; D015169:Colony Count, Microbial; D008081:Liposomes; D008172:Lung Diseases, Fungal / Q000188:drug therapy* / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D008081:Liposomes; D000666:Amphotericin B; D000687:Amylopectin