Differential effects of two alleles of the dy locus on the pituitary-testicular axis of mice.
Testicular function was studied in vivo and in vitro in adult male dy/dy and dy2J/dy2J dystrophic mice. The results demonstrate that testicular function in dy/dy mice is more affected. The basal levels of pituitary hormones measured were normal in dystrophic mice, except for the presence of hyperprolactinemia in dy/dy mice. In dy/dy mice testicular weight was diminished and a deficient transduction of the gonadotropic signal is present in vivo, accompanied by reduced efficiency of 17-hydroxylase and 17-hydroxysteroid dehydrogenase. In dy2J/dy2J mice the signal transduction is normal and the reduction in enzyme efficiency is limited to 17-hydroxysteroid dehydrogenase. The in vitro HCG-induced increases in production of testosterone (T) and estradiol (E2) were reduced in dy/dy/mice, and the data indicate a reduction of enzyme activity rather than in efficiency. In dy21/dy21/mice, HCG-induced T synthesis was increased, HCG-induced E2 synthesis was normal, but basal media E2 levels were reduced, with the in vitro efficiency of aromatase being suppressed under both basal and HCG-stimulated conditions, when compared to their normal littermates.
Amador
A G
AG
Department of Obstetrics & Gynecology, SIU School of Medicine, Springfield 62794-9230.
Mayerhofer
A
A
Parkening
T A
TA
Collins
T J
TJ
Bartke
A
A
D015067:17-Hydroxysteroid Dehydrogenases / Q000172:deficiency*; D000312:Adrenal Hyperplasia, Congenital; D000483:Alleles; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004195:Disease Models, Animal; D012739:Gonadal Steroid Hormones / Q000032:analysis; D006966:Hyperprolactinemia / Q000209:etiology; D007030:Hypothalamo-Hypophyseal System / Q000503:physiopathology; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains / Q000235:genetics / Q000502:physiology*; D009137:Muscular Dystrophy, Animal / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D009924:Organ Culture Techniques; D010903:Pituitary Gland, Anterior / Q000503:physiopathology*; D010908:Pituitary Hormones, Anterior / Q000097:blood; D011974:Receptors, LH / Q000032:analysis; D013045:Species Specificity; D013737:Testis / Q000187:drug effects / Q000201:enzymology / Q000503:physiopathology*; D013739:Testosterone / Q000032:analysis; D049411:Utrophin
D006063:Chorionic Gonadotropin; D003598:Cytoskeletal Proteins; D012739:Gonadal Steroid Hormones; D008565:Membrane Proteins; D010908:Pituitary Hormones, Anterior; D011974:Receptors, LH; C483600:Utrn protein, mouse; D049411:Utrophin; D013739:Testosterone; D015067:17-Hydroxysteroid Dehydrogenases