A cDNA clone representative of a novel splicing pattern of the D. melanogaster dunce gene.
The D. melanogaster dunce gene is involved in both the learning and memory processes of the fly. The gene encodes for a cAMP-specific phosphodiesterase, a function playing a central role in the regulation of the intracellular cAMP level. Molecular cloning of dunce has so far not been completely achieved, although it is known that the gene encodes a large set of RNAs and has a complex organization, extending for more than 140 kilobases and containing several genes within its introns. Here we report the isolation and the characterization of 21/7, a cDNA clone representative of a novel dunce splicing pattern. The nucleotide sequence of this clone led to the identification of a dunce exon included in at least one transcript so far uncharacterized.
Furia
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M
Dipartimento di Genetica, Università di Napoli, Italy.
Giordano
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E
Digilio
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A
Polito
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LC
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics*; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000235:genetics*; D005091:Exons; D005786:Gene Expression Regulation; D007858:Learning; D008568:Memory; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012313:RNA; D015105:3',5'-Cyclic-AMP Phosphodiesterases