title: cis and trans regulation of tissue-specific transcription.
abstract: Analysis of both the cis-regulatory sequences which control globin gene switching as well as the trans-acting factors which bind to these sequences to elicit a differential, developmentally regulated response has lent insight into the general mechanisms responsible for tissue-specific gene regulation. We show here that the chicken adult beta-globin gene promoter sequences are intimately involved in competitive interaction with the beta/epsilon-globin enhancer to regulate differentially epsilon- versus beta-globin gene transcription. Secondly, we show that the family of GATA transcription factors directs gene regulation in a variety of discrete cell types, and describe potential cellular target genes for each member of the GATA factor family, as well as potential mechanisms whereby multiple GATA factors expressed in a single cell might be used to elicit differential transcriptional activities.
authors.affiliation: Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208-3500.
authors.forename: J D
authors.initials: JD
authors.lastname: Engel
authors.forename: H
authors.initials: H
authors.lastname: Beug
authors.forename: J H
authors.initials: JH
authors.lastname: LaVail
authors.forename: M W
authors.initials: MW
authors.lastname: Zenke
authors.forename: K
authors.initials: K
authors.lastname: Mayo
authors.forename: M W
authors.initials: MW
authors.lastname: Leonard
authors.forename: K P
authors.initials: KP
authors.lastname: Foley
authors.forename: Z
authors.initials: Z
authors.lastname: Yang
authors.forename: J M
authors.initials: JM
authors.lastname: Kornhauser
authors.forename: L J
authors.initials: LJ
authors.lastname: Ko
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001921:Brain / Q000196:embryology; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000235:genetics*; D050981:Erythroid-Specific DNA-Binding Factors; D005786:Gene Expression Regulation; D005812:Genes, Switch; D005914:Globins / Q000235:genetics*; D006678:HIV / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D014157:Transcription Factors; D005914:Globins