title: The synthetic hepatic peptides pyroglutamylglutamylglycylserylasparagine and pyroglutamylglutamylglycylserylaspartic acid inhibit growth of MH1C1 rat hepatoma cells transplanted into Buffalo rats or athymic mice.
abstract: Repeated i.p. injections of the synthetic peptides pyroglutamylglutamylglycylserylasparagine and pyroglutamylglutamylglycylserylaspartic acid inhibited the long-term growth of MH1C1 rat hepatoma cells by 50-70% in three in vivo models: metastatic colony growth in the lungs of young Buffalo rats; s.c. tumor growth in young Buffalo rats; and s.c. tumor growth in athymic mice. The amide free peptide pyroglutamylglutamylglycylserylaspartic acid which inhibited the tumor growth in all the models showed a curvilinear dose-response relationship with a maximal effect at 1000 pmol/animal in mice and at 100 pmol/animal in rats. The amidated peptide pyroglutamylglutamylglycylserylasparagine, which was only tested in the lung model, showed growth inhibition with 2, 20, or 200 pmol/animal, but 200 pmol/animal was most effective. We have recently reported that these peptides show cochromatography with hepatic growth inhibitory peptides, isolated from mouse liver.
authors.affiliation: Institute of Pathology, Rikshospitalet, Oslo, Norway.
authors.forename: J E
authors.initials: JE
authors.lastname: Paulsen
authors.forename: K S
authors.initials: KS
authors.lastname: Hall
authors.forename: H E
authors.initials: HE
authors.lastname: Rugstad
authors.forename: K L
authors.initials: KL
authors.lastname: Reichelt
authors.forename: K
authors.initials: K
authors.lastname: Elgjo
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000517:prevention & control* / Q000556:secondary*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005260:Female; D008175:Lung Neoplasms / Q000517:prevention & control* / Q000556:secondary*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009842:Oligopeptides / Q000494:pharmacology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D011915:Rats, Inbred BUF
chemical_list: D009842:Oligopeptides; C072903:pyroglutamyl-glutamyl-glycyl-seryl-asparagine; C072904:pyro-glutamyl-glutamyl-glycyl-seryl-aspartic acid; D011761:Pyrrolidonecarboxylic Acid