[Differential pulse voltammetric measurements of cholesterol based on its oxidation after adsorption at the mercury electrode].
Cholesterol can be adsorbed on the surface of the static mercury drop electrode, and then oxidized during anodic potential scan. The stripping peak potential in differential pulse voltammetry is at -0.08 V in 0.1 mol/L K2HPO4-KH2PO4 buffer solution. The stripping current is linear with the concentration of cholesterol in the range of 10(-7)-10(-6) mol/L. The detection limit found is 8 x 10(-8) mol/L after a 5 min pre-concentration period. The adsorption and oxidation mechanisms of cholesterol at the mercury electrode were then explored. The experimental conditions for measuring free cholesterol in human sera were examined.
Peng
T Z
TZ
Department of Chemistry, Hangzhou University.
Li
H P
HP
Lu
R S
RS
D000327:Adsorption; D002784:Cholesterol / Q000097:blood* / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D010084:Oxidation-Reduction
D002784:Cholesterol