title: [The effect of chemical agents on lysosome fusion with phagosomes and on the F-actin content in murine peritoneal macrophages].
abstract: The influence of natural and synthetic polyamines, phalloidin, cytochalasin D, vinblastine, colchicine, puromycin, chlorpromazine, urea, glutaraldehyde, and ethanol on the phagosome-lysosome fusion and the content of F-actin in murine peritoneal macrophages has been studied. Fluorescent phallotoxin FITC-phalloidin was used to stain F-actin. Natural polyamines (spermine, spermidine, putrescine), phalloidin, ethanol (0.1 M) stimulated the phagosome-lysosome fusion and increased the mid-content of F-actin in macrophages. Cytochalasin D, vinblastine, colchicine, puromycin, chlorpromazine, urea, glutaraldehyde, ethanol (0.15 and 0.2 M) inhibited this process and decreased the mid-content of F-actin. Possible mechanisms of the interconnection of cytoskeleton and the phagosome-lysosome fusion are discussed.
authors.forename: T P
authors.initials: TP
authors.lastname: Mozhenok
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Rpozanov
authors.forename: L V
authors.initials: LV
authors.lastname: Solov'eva
authors.forename: A D
authors.initials: AD
authors.lastname: Braun
authors.forename: A G
authors.initials: AG
authors.lastname: Bulychev
mesh_terms: D000199:Actins / Q000032:analysis / Q000187:drug effects*; D000818:Animals; D002459:Cell Fusion; D002478:Cells, Cultured / Q000187:drug effects / Q000648:ultrastructure; D008247:Lysosomes / Q000187:drug effects* / Q000648:ultrastructure; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity / Q000166:cytology; D010588:Phagosomes / Q000187:drug effects* / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
chemical_list: D000199:Actins
vernacular_title: Vliianie nekotorykh agentov na sliianie lizosom s fagosomami i na soderzhanie F-aktina v peritoneal'nykh makrofagakh mysheĭ.