title: Phagocytosis of Porphyromonas gingivalis and Prevotella intermedia by human polymorphonuclear leukocytes in clots formed from human plasma or purified fibrinogen.
abstract: We have studied the polymorphonuclear leukocyte (PMN) phagocytosis of Porphyromonas gingivalis (Bacteroides gingivalis) and Prevotella intermedia (Bacteroides intermedius) in three-dimensional fibrin meshworks formed by either clotting human plasma or by clotting purified fibrinogen with human serum incorporated. PMN phagocytosis of rhodamine isothiocyanate-labelled P. gingivalis in the fibrinogen clot was 43 +/- 6% and in the plasma clot was 62 +/- 10%; for P. intermedia the values were 15 +/- 10% and 63 +/- 10%, respectively. This showed that phagocytosis in the plasma clot system was significantly higher than in the fibrinogen clot system. However, increasing the concentration of serum in the fibrinogen clot abolished this difference, suggesting that opsonin levels determine PMN function in this assay. Labelling the bacteria with a different fluorochrome, fluoroscein isothiocyanate, reduced the level of PMN phagocytosis in both three-dimensional systems but phagocytosis was unaffected when PMN were adherent to glass. It would appear that PMN phagocytosis in three-dimensional systems can vary depending on the composition of the clot and the fluorochrome used to label the bacteria.
authors.affiliation: Medical Research Council, Dental Research Unit, London, U.K.
authors.forename: T J
authors.initials: TJ
authors.lastname: Hurst
authors.forename: J M
authors.initials: JM
authors.lastname: Wilton
mesh_terms: D000704:Analysis of Variance; D001439:Bacteroides / Q000276:immunology*; D005337:Fibrin; D005340:Fibrinogen / Q000502:physiology*; D005353:Fibronectins / Q000502:physiology; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology; D005883:Gingival Crevicular Fluid / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D008954:Models, Biological; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects; D010949:Plasma / Q000502:physiology*; D016966:Porphyromonas gingivalis / Q000276:immunology; D012235:Rhodamines / Q000494:pharmacology
chemical_list: D005353:Fibronectins; D009895:Opsonin Proteins; D012235:Rhodamines; D005337:Fibrin; D005340:Fibrinogen; C027755:rhodamine isothiocyanate; D016650:Fluorescein-5-isothiocyanate