Telomere-associated chromosome fragmentation: applications in genome manipulation and analysis.
Telomere-associated chromosome fragmentation (TACF) is a new approach for chromosome mapping based on the non-targeted introduction of cloned telomeres into mammalian cells. TACF has been used to generate a panel of somatic cell hybrids with nested terminal deletions of the long arm of the human X chromosome, extending from Xq26 to the centromere. This panel has been characterized using a series of X chromosome loci. Recovery of the end clones by plasmid rescue produces a telomeric marker for each cell line and partial sequencing will allow the generation of sequence tagged sites (STSs). TACF provides a powerful and widely applicable method for genome analysis, a general way of manipulating mammalian chromosomes and a first step towards constructing artificial mammalian chromosomes.
Farr
C J
CJ
Imperial Cancer Research Fund, London, UK.
Stevanovic
M
M
Thomson
E J
EJ
Goodfellow
P N
PN
Cooke
H J
HJ
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005821:Genetic Techniques; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites; D016615:Telomere / Q000648:ultrastructure*; D014960:X Chromosome / Q000648:ultrastructure*
D015342:DNA Probes; D004247:DNA