Iron metabolism in Pseudomonas: salicylic acid, a siderophore of Pseudomonas fluorescens CHAO.
Under iron-starvation conditions of growth, Pseudomonas fluorescens CHA0, a soil isolate involved in phytopathogenic fungi antagonisms, produced, together with pyoverdine, a second iron-chelating compound which was purified and identified by spectroscopy, HPLC and 1H-NMR to be salicylic acid. Mutants unable to synthesize pyoverdine overproduced this compound by a factor of 9-14. The biosynthesis of salicylic acid was under iron control; it was fully inhibited by 5 microM added iron in the growth medium. In contrast, salicylic acid of either bacterial or commercial origin facilitated labeled iron incorporation in iron-starved cells. Based on these two relationships observed with bacterial iron metabolism it is concluded that salicylic acid has a siderophore function for this strain.
Meyer
J M
JM
Laboratoire de Microbiologie, IBMC du CNRS, Université Louis Pasteur, Strasbourg, France.
Azelvandre
P
P
Georges
C
C
D007501:Iron / Q000378:metabolism*; D009842:Oligopeptides; D010636:Phenols / Q000378:metabolism; D010860:Pigments, Biological / Q000096:biosynthesis; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D012459:Salicylates / Q000302:isolation & purification / Q000378:metabolism*; D020156:Salicylic Acid; D017262:Siderophores / Q000096:biosynthesis* / Q000302:isolation & purification / Q000502:physiology; D013844:Thiazoles
D009842:Oligopeptides; D010636:Phenols; D010860:Pigments, Biological; D012459:Salicylates; D017262:Siderophores; D013844:Thiazoles; C025316:pyochelin; C042453:pyoverdin; D007501:Iron; D020156:Salicylic Acid