title: Automatic measurement of hemoglobin F in blood obtained from patients with hemoglobin E/E and beta-thalassemia/hemoglobin/E.
abstract: This paper presents an automated determination of hemoglobin (Hb) F in Hb E/E disease using Hi-Auto A1c. Blood specimens collected in Bangkok were frozen, and sent to Japan by air mail for the determination. The automatically determined values showed a high correlation with the values obtained by the classical alkali denaturation method. Hb E/E cases showed 4.24 +/- 1.75% of Hb F. On the other hand, Hb, Hct, MCV and MCH in the disease samples were lower than in the controls, but higher than those of beta-thalassemia/HbE disease. From the results it was concluded that Hb E/E could be differentiated from beta-thalassemia/HbE by combination of Hb F value and MCH or Hb in CBC.
authors.affiliation: Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Tatsumi
authors.forename: I
authors.initials: I
authors.lastname: Tsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Funahara
authors.forename: A
authors.initials: A
authors.lastname: Bunyaratvej
authors.forename: S
authors.initials: S
authors.lastname: Fucharoen
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D003937:Diagnosis, Differential; D004906:Erythrocyte Count; D004909:Erythrocyte Indices; D005069:Evaluation Studies as Topic; D005312:Fetal Blood / Q000737:chemistry; D005319:Fetal Hemoglobin / Q000737:chemistry*; D006400:Hematocrit; D006446:Hemoglobin E; D006453:Hemoglobinopathies / Q000097:blood* / Q000150:complications / Q000453:epidemiology; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D007231:Infant, Newborn; D015203:Reproducibility of Results; D013785:Thailand / Q000453:epidemiology; D017086:beta-Thalassemia / Q000097:blood* / Q000150:complications / Q000453:epidemiology
chemical_list: D006454:Hemoglobins; D006446:Hemoglobin E; D005319:Fetal Hemoglobin