[Anchored cell analysis and sorting (ACAS) system].
Advanced biology and recent technology have provided sophisticated and objective method for analyzing biological characteristics on cells. Following that, many new instruments have developed. Diagnostic immunocytochemistry has become an accepted diagnostic tool in cell biology. In recent years, remarkable advances in technology provide a method for quantitative and objective analyses of cell characteristics. The newly developed computer assisted laser cytometer (ACAS 570) can be applied in clinical basis as well as in research laboratory. Fluorescent intensities of ancharage-dependent cells can be automatically analyzed and make it possible to separate a subpopulation of cells. This computer controlled system principally consists of argon ion laser, phase contrast microscope. Quantitative fluorescence measurements and computer graphic images can be obtained. The present paper demonstrates multiple applications of laser cytometer for evaluation of cell biology.
Miyakawa
A
A
Department of Urology, School of Medicine, Keio University, Tokyo, Japan.
Tachibana
M
M
Tazaki
H
H
D000951:Antigens, Neoplasm / Q000032:analysis; D002469:Cell Separation / Q000379:methods*; D003201:Computers; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007834:Lasers; D008562:Membrane Glycoproteins / Q000032:analysis; D008858:Microscopy, Phase-Contrast; D010545:Peroxides / Q000032:analysis; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D008562:Membrane Glycoproteins; D010545:Peroxides