title: Expression of high affinity binding sites for erythropoietin on L8057 cells, a mouse megakaryoblastic cell line, associated with cell differentiation.
abstract: In this study, specific binding sites were examined for erythropoietin (EPO) on the mouse leukemic cell line, L8057. This cell line is megakaryoblastic in origin as evidenced by an enlargement of cell size, multinuclearity, intense activity of acetylcholinesterase, more expression of glycoprotein IIb and IIIa antigen, and higher ploidy distribution after the treatment with 12-o-tetradecanoylphorbor-13-acetate (TPA). The original undifferentiated cells possessed a single class of low affinity binding sites for recombinant human (rh) EPO with a Kd of 3.5 nM. Following the treatment with TPA, high affinity binding sites (Kd; 440 pM) were expressed in addition to the low affinity sites. EPO stimulated the incorporation of 3H-leucine into TPA-treated L8057 cells, and the maximal effect of EPO was observed at the same order as the Kd value of high affinity sites. The present data demonstrates that the expression of high affinity binding sites for EPO is associated with the differentiation of L8057 cells which have megakaryocytic characteristics. Furthermore, protein synthesis stimulated by EPO may be mediated through the high affinity sites.
authors.affiliation: Third Department of Internal Medicine, Yamaguchi University School of Medicine, Ube, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Ishida
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Kaku
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000941:Antigens / Q000032:analysis / Q000276:immunology; D002454:Cell Differentiation / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics; D004921:Erythropoietin / Q000378:metabolism* / Q000502:physiology; D007457:Iodine Radioisotopes; D007930:Leucine / Q000378:metabolism; D007942:Leukemia, Experimental / Q000378:metabolism / Q000473:pathology*; D007947:Leukemia, Megakaryoblastic, Acute; D008533:Megakaryocytes / Q000378:metabolism* / Q000473:pathology* / Q000648:ultrastructure; D051379:Mice; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000032:analysis / Q000378:metabolism*; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins / Q000378:metabolism / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000378:metabolism / Q000473:pathology
chemical_list: D000941:Antigens; D004273:DNA, Neoplasm; D007457:Iodine Radioisotopes; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins; D004921:Erythropoietin; D000110:Acetylcholinesterase; D007930:Leucine; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine