Expression of cloned aromatic L-amino acid decarboxylase in Xenopus laevis oocytes.
Sense mRNA coding for bovine adrenal medulla aromatic L-amino acid decarboxylase (AADC) was expressed following microinjection into Xenopus laevis oocytes. The expressed enzyme activity was stereoselective for L-5-hydroxytryptophan and L-DOPA and blocked by NSD-1015 an inhibitor of AADC. Heating the expressed enzyme at 55 degrees C resulted in a parallel loss of activity towards both substrates. Our findings are consistent with the prevailing notion that a single enzyme is able to decarboxylate both substrates in vivo.
Gudehithlu
K P
KP
Department of Pharmacology, Ohio State University College of Medicine, Columbus 43210.
Duchemin
A M
AM
Silvia
C P
CP
Neff
N H
NH
Hadjiconstantinou
M
M
D000311:Adrenal Glands / Q000201:enzymology; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000096:biosynthesis* / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015723:Gene Library; D007700:Kinetics; D008845:Microinjections; D009865:Oocytes / Q000502:physiology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000008:administration & dosage / Q000378:metabolism*; D014158:Transcription, Genetic; D014982:Xenopus laevis
D012333:RNA, Messenger; D004247:DNA; D001142:Aromatic-L-Amino-Acid Decarboxylases