Assignments of Raman tensors to Raman bands of nucleic acids: use of a single crystal of adenosine triphosphoric acid and DNA fibers.
A polarized laser (488.0 nm) Raman spectroscopic measurement has been made of a single crystal of adenosine triphosphoric acid trihydrate (ATP.3H2O), whose space group is P2(1)2(1)2(1), and whose dimensions are about 10 microns x 100 microns x 100 microns along the crystallographic a, b and c axes, respectively. For each Raman band in the 300-1800 cm-1 range, relative scattering intensities, Ibb and Icc, which correspond to the bb and cc components of the Raman tensor of the crystal, have been determined. In an aqueous solution of ATP, the depolarization ratio of each Raman band has also been determined. On the basis of these experimental results, some discussions are given of the shapes and orientations of the Raman scattering tensors localized in the base residue, ribose portion, and phosphodiester portions. A similar type of study has been made with a few DNA fibers.
Tsuboi
M
M
Department of Fundamental Science, Iwaki Meisei University, Fukushima, Japan.
Ushizawa
K
K
Ueda
T
T
D000255:Adenosine Triphosphate / Q000737:chemistry*; D003460:Crystallization; D004247:DNA / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014867:Water / Q000737:chemistry
D014867:Water; D000255:Adenosine Triphosphate; D004247:DNA