Altering the insertional specificity of a Drosophila transposable element.
Vectors derived from the Drosophila P element transposon are widely used to make transgenic Drosophila. Insertion of most P-element-derived vectors is nonrandom, but they exhibit a broad specificity of target sites. During experiments to identify cis-acting regulatory elements of the Drosophila segmentation gene engrailed, we identified a fragment of engrailed DNA that, when included within a P-element vector, strikingly alters the specificity of target sites. P-element vectors that contain this fragment of engrailed regulatory DNA insert at a high frequency near genes expressed in stripes.
Kassis
J A
JA
Laboratory of Cellular and Molecular Biology, Food and Drug Administration, Bethesda, MD 20892.
Noll
E
E
VanSickle
E P
EP
Odenwald
W F
WF
Perrimon
N
N
D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005796:Genes; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic
D004251:DNA Transposable Elements; D009838:Oligodeoxyribonucleotides