[The effect of nitroglycerin, nitrosorbide and nitroprusside on the intracellular content of Ca2+ ions in human lymphocytes].
We used Fura-2 fluorometry to observe the effects of nitroglycerin (NG), isosorbide--2,5--dinitrate (ISDN) and sodium nitroprusside (NP) on basal and mitogen-induced (Con A, 25 micrograms/ml) cytoplasmic Ca++level ([Ca++]) in human blood lymphocytes. It is shown that nitrates dose-dependently decrease Con-A-induced increase in [Ca++]i. When compared for the effect on calcium metabolism, NP appeared most potent (IC50 = 4.1 x 10(-7) M). The drug action was not reported when the cells were exposed to Ca(++)-free solution. 120-min preincubation with 10(-4) M NG led to complete abolishment of the ISDN and NG effects. The results suggest that human lymphocytes may be used as an experimental model of nitrates action and mechanisms underlying tolerance to them.
Sergeev
P V
PV
Storozhakov
G I
GI
Dukhanin
A S
AS
Shilo
V Iu
VIu
Shimanovskiĭ
N L
NL
Khamitova
E S
ES
Trepilets
V E
VE
D002118:Calcium / Q000097:blood*; D003208:Concanavalin A / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D006801:Humans; D007548:Isosorbide Dinitrate / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D005996:Nitroglycerin / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology*; D013997:Time Factors
D005456:Fluorescent Dyes; C049925:fura-2-am; D003208:Concanavalin A; D009599:Nitroprusside; D005996:Nitroglycerin; D007548:Isosorbide Dinitrate; D002118:Calcium; D016257:Fura-2
Vliianie nitroglitserina, nitrosorbida i nitroprussida na vnutrikletochnoe soderzhanie ionov Ca2+ v limfotsitakh cheloveka.