title: Significant factors in the disinfection and sterilization of flexible endoscopes.
abstract: BACKGROUND
  Many nosocomial infection outbreaks have been linked to improper disinfection of the flexible endoscopes used in hospitals and clinics. The objective of this study was to evaluate the efficacy of scope disinfection with glutaraldehyde and hydrogen peroxide in manual and mechanical protocols.
  
  
  METHODS
  Bacillus subtilis and Pseudomonas cepacia were the test organisms. Each channel in two different endoscopes was seeded and evaluated separately. Residual chemical germicide levels in the channels and in the work environment were also measured.
  
  
  RESULTS
  Parametric analyses were carried out on log transformations of number of colony-forming units recovered. Repeated measures analysis demonstrated that both the type of disinfectant and the method of washing were significant factors for disinfection.
  
  
  CONCLUSIONS
  Hydrogen peroxide proved to be more efficacious than glutaraldehyde for killing or removing B. subtilis in a 10-minute contact period. Automatic disinfection was more efficacious than manual disinfection for killing or removing B. subtilis in a 10-minute contact period. The channel being disinfected also proved to be a significant factor, with carbon dioxide and elevator channels the most difficult to disinfect consistently.
authors.affiliation: School of Public Health, University of Minnesota, Minneapolis 55455.
authors.forename: D
authors.initials: D
authors.lastname: Vesley
authors.forename: K G
authors.initials: KG
authors.lastname: Norlien
authors.forename: B
authors.initials: B
authors.lastname: Nelson
authors.forename: B
authors.initials: B
authors.lastname: Ott
authors.forename: A J
authors.initials: AJ
authors.lastname: Streifel
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects; D016956:Burkholderia cepacia / Q000187:drug effects; D002495:Central Supply, Hospital; D004203:Disinfection / Q000379:methods*; D019723:Endoscopes; D004866:Equipment Contamination; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D013242:Sterilization / Q000379:methods*
chemical_list: D006861:Hydrogen Peroxide; D005976:Glutaral