A biocompatible needle-type glucose sensor based on platinum-electroplated carbon electrode.
A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a disposable hypodermic needle made of stainless steel as the counter electrode. A Nafion membrane, an immobilized glucose oxidase (GOD) membrane, and a biocompatible membrane with diffusion-limiting effect were coated successively onto the working electrode. The sensor showed a rapid response (< 120 s in batch operation), good reproducibility (RE < 3%), good stability (over 36 h in control serum), a wide dynamic range (5-600 mg/dL glucose), and superior biocompatibility. It was used to determine glucose in serum. The data obtained from the sensor showed good agreement with that from a clinical autoanalyzer (R > 0.95).
Chen
C Y
CY
Research Center for Advanced Science and Technology, University of Tokyo, Japan.
Tamiya
E
E
Ishihara
K
K
Kosugi
Y
Y
Su
Y C
YC
Nakabayashi
N
N
Karube
I
I
D001672:Biocompatible Materials; D015374:Biosensing Techniques / Q000592:standards; D001786:Blood Glucose / Q000032:analysis*; D002138:Calibration; D002244:Carbon; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials; D008567:Membranes, Artificial; D008670:Metals / Q000494:pharmacology; D009339:Needles; D010984:Platinum; D011108:Polymers; D015203:Reproducibility of Results; D013696:Temperature
D001672:Biocompatible Materials; D001786:Blood Glucose; D008567:Membranes, Artificial; D008670:Metals; D011108:Polymers; D010984:Platinum; D002244:Carbon