Characterization of parathyroid hormone-related protein in the human term placenta.
To characterize parathyroid hormone-related protein (PTHrP) in the human placenta, we measured PTHrP-like immunoreactivity (PRP-LI) in the term placenta and studied the elution profiles of placental tissue extracts on Sephadex G-75 chromatography with a specific RIA. We also examined the gene expression of PTHrP mRNA by Northern blot analysis and the localization of PRP-LI in the placenta by immunohistochemistry. The amount of PRP-LI in placental extracts (n = 7) was 20.9 +/- 2.2 pg/g wet tissue (mean +/- SE). Dilution curves of placental tissue ran parallel to those of synthetic PTHrP (1-34) standards. Sephadex G-75 gel chromatography demonstrated two major PRP-LI peaks; the first peak was eluted around the molecular size between 10 kilodaltons (Kda) and 20 Kda and the other around 5 Kda. Northern blot analysis of PTHrP mRNA extracted from placental tissues showed a major hybridization signal around 18S. PTHrP immunohistochemistry showed PRP-LI staining in the cytoplasm of syncytiotrophoblasts and stroma cells (Hofbauer cells) in the term placenta. These results suggest that syncytiotrophoblasts and stroma cells in the term placenta synthesize PTHrP in two major molecular forms, 10 Kda-20 Kda and around 5 Kda.
Ishikawa
E
E
Department of Internal Medicine, Miyazaki Medical College, Japan.
Katakami
H
H
Hidaka
H
H
Ushiroda
Y
Y
Ikeda
T
T
Ikenoue
T
T
Matsukura
S
S
D000328:Adult; D015152:Blotting, Northern; D002850:Chromatography, Gel; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D044162:Parathyroid Hormone-Related Protein; D010920:Placenta / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D011863:Radioimmunoassay
C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins; D012333:RNA, Messenger