Isolation of an avianlike group A rotavirus from a calf with diarrhea.
An atypical group A rotavirus (993/83) was isolated from a 3-day-old German calf with diarrhea. It differed from 35 conventional German bovine rotavirus isolates analyzed previously with respect to subgroup (strain 993/83 was non-subgroup I and non-subgroup II), serotype (strain 993/83 showed a two-way cross-reaction with serotype 7 and a one-way cross-reaction with serotype 3), and electropherotype (strain 993/83 showed comigrating gene segments 10 and 11). Isolate 993/83 reacted with only one of four monoclonal antibodies that recognized a common VP6 epitope(s). In addition, VP6 and VP2 of isolate 993/83 showed one-dimensional peptide maps that differed substantially from the peptide maps of VP6 and VP2 from all bovine rotavirus isolates. By RNA-RNA hybridization, the 993/83 probe failed to react with a panel of mammalian rotavirus strains, including bovine rotaviruses. It hybridized, however, to genomic RNA of an avian rotavirus strain. Isolate 993/83 could thus represent a candidate for a natural interspecies transmission of rotavirus between different classes of vertebrates.
Brüssow
H
H
Nestlé Research Centre, Nestec Ltd., Lausanne, Switzerland.
Nakagomi
O
O
Gerna
G
G
Eichhorn
W
W
D000818:Animals; D000831:Animals, Newborn; D000956:Antigens, Viral; D002213:Capsid / Q000737:chemistry / Q000276:immunology; D036022:Capsid Proteins; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004586:Electrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D006168:Guinea Pigs; D006801:Humans; D009500:Neutralization Tests; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D012400:Rotavirus Infections / Q000662:veterinary*; D012703:Serotyping
D000956:Antigens, Viral; D036022:Capsid Proteins; D010455:Peptides; D012367:RNA, Viral; C442545:VP2 protein, Rotavirus; C061332:VP6 protein, Rotavirus