Characterization of DNA sequences that mediate nuclear protein binding to the regulatory region of the Pisum sativum (pea) chlorophyl a/b binding protein gene AB80: identification of a repeated heptamer motif.
Two protein factors binding to the regulatory region of the pea chlorophyl a/b binding protein gene AB80 have been identified. One of these factors is found only in green tissue but not in etiolated or root tissue. The second factor (denominated ABF-2) binds to a DNA sequence element that contains a direct heptamer repeat TCTCAAA. It was found that presence of both of the repeats is essential for binding. ABF-2 is present in both green and etiolated tissue and in roots and factors analogous to ABF-2 are present in several plant species. Computer analysis showed that the TCTCAAA motif is present in the regulatory region of several plant genes.
Argüello
G
G
CINVESTAV, I.P.N., U. Irapuato., Department of Genetic Engineering, Gto., Mexico.
García-Hernández
E
E
Sánchez
M
M
Gariglio
P
P
Herrera-Estrella
L
L
Simpson
J
J
D001483:Base Sequence; D001665:Binding Sites; D002734:Chlorophyll / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D007887:Fabaceae / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D017343:Genes, Plant; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000235:genetics* / Q000378:metabolism*; D010946:Plants, Medicinal; D011485:Protein Binding; D012091:Repetitive Sequences, Nucleic Acid; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014157:Transcription Factors
C072843:ABF2 protein, S cerevisiae; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D002734:Chlorophyll; D004247:DNA