Effects of dentin proteins, transforming growth factor beta 1 (TGF beta 1) and bone morphogenetic protein 2 (BMP2) on the differentiation of odontoblast in vitro.
We have studied the effects of dentin proteins, of Transforming Growth Factor beta 1 (TGF beta 1) and Bone Morphogenetic Protein (BMP2) on the differentiation of odontoblasts in vitro. The total EDTA-soluble fraction of dentin proteins, prepared from rabbit incisors was further separated by chromatography on DEAE-Cellulose and heparin-agarose columns. While the total EDTA-soluble fraction of dentin had no effect on cultured dental papillae, fractions retained on both columns were able to initiate functional differentiation of preodontoblasts of isolated day-17 first lower mouse molar dental papillae cultured in vitro. TGF beta 1 and BMP2, both stimulated the matrix secretion by dental papillae cells. TGF beta 1 and BMP2, combined with the inactive total EDTA-soluble fraction, stimulated odontoblast differentiation. An active fraction retained on DEAE-Cellulose completely lost the inductive activity after incubation with a neutralizing anti-TGF beta antibody. These results demonstrate that a TGF beta-like molecule present in dentin could interact with some component which acts as a modulator of its activity on the initiation of the cytological and functional differentiation of odontoblasts.
Bègue-Kirn
C
C
Institut de Biologie Médicale, Faculté de Médecine, Strasbourg, France.
Smith
A J
AJ
Ruch
J V
JV
Wozney
J M
JM
Purchio
A
A
Hartmann
D
D
Lesot
H
H
D000818:Animals; D019485:Bone Morphogenetic Proteins; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D003804:Dentin; D051379:Mice; D009804:Odontoblasts / Q000187:drug effects*; D011506:Proteins / Q000494:pharmacology*; D014083:Tooth Germ / Q000196:embryology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D019485:Bone Morphogenetic Proteins; D011506:Proteins; D016212:Transforming Growth Factor beta