Collagen-induced rat platelet reactivity is enhanced in whole blood in both the presence and absence of dense granule secretion.
Collagen induced aggregation, ATP secretion and thromboxane (TxB2) generation of storage pool deficient platelets were compared to normal platelets of closely related rat strains. Platelet function was monitored in citrated-platelet-rich-plasma (PRP) and citrated whole blood. Wistar (W) and fawn-hooded (FH) rat strains and their F2 hybrids were utilized. The W strain, which is ancestral to the FH strain, is not storage pool deficient while the FH strain is. This was manifested by the total lack of collagen induced ATP secretion from platelets of the FH strain while the platelets of the W strain secreted normally. Utilizing platelets from the F2 generation of WxFH matings, the absence of dense granule secretion (ATP) from the FH platelets, as well as other platelet defects of FH rats, were shown to be associated with homozygosity for the red-eyed dilution gene [r]. The non-secreting FH platelets were utilized to determine the effects of secreted dense granule constituents upon collagen induced aggregation and TxB2 generation. The non-secreting storage pool deficient platelets did aggregate and did generate TxB2 upon stimulation with collagen; however, the storage pool deficient FH platelets demonstrated less TxB2 generation and did not aggregate as effectively as the normally secreting platelets of the W strain. When evaluating collagen induced platelet function in whole blood as compared to PRP, the storage pool deficient platelets remained less reactive than normally secreting platelets, but both platelet types demonstrated enhanced aggregation and increased TxB2 generation in whole blood.
Magro
A
A
Fairmont State College, WV.
Bizios
R
R
Catalfamo
J
J
Blumenstock
F
F
Rudofsky
U
U
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D003094:Collagen / Q000494:pharmacology*; D005838:Genotype; D008297:Male; D010974:Platelet Aggregation; D010981:Platelet Storage Pool Deficiency / Q000503:physiopathology*; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics; D017208:Rats, Wistar / Q000235:genetics; D013928:Thromboxane A2 / Q000096:biosynthesis
D013928:Thromboxane A2; D000255:Adenosine Triphosphate; D003094:Collagen