Expression of Mn-superoxide dismutase in carcinogenesis.
Human liver manganese superoxide dismutase (Mn-SOD) was highly purified by a simple procedure and crystallized. A monoclonal antibody against Mn-SOD, whose antigen-binding epitope is a C-terminus peptide was developed. Using this antibody, an enzyme-linked immunosorbent assay (ELISA) was developed. We found that Mn-SOD is highly expressed in human ovarian cancer and the serum level of the enzyme is a useful marker for the diagnosis and monitoring of the epithelial type of ovarian cancer. Tumor necrosis factor-alpha (TNF), lipopolysaccharide, IL-1 and phorbol ester induced the m-RNA of Mn-SOD as well as protein levels in TNF-resistant cells. No such induction was observed in Cu, Zn-SOD. Studies on the induction mechanisms indicated that at least two separate signal-transducing pathways are involved in expression of the Mn-SOD gene. One is triggered by protein kinase C activation itself in the absence of new protein synthesis. The other can be activated by stimulations with various cytokines in which a protein factor that can be induced by phorbol ester treatments is involved.
Taniguchi
N
N
Department of Biochemistry, Osaka University Medical School, Suita, Japan.
Ishikawa
M
M
Kawaguchi
T
T
Fujii
J
J
Suzuki
K
K
Nakata
T
T
D014408:Biomarkers, Tumor / Q000097:blood*; D002278:Carcinoma in Situ / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008099:Liver / Q000201:enzymology*; D010051:Ovarian Neoplasms / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000032:analysis* / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D014408:Biomarkers, Tumor; D014409:Tumor Necrosis Factor-alpha; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate