Phaseolus vulgaris L. var. HUR 15--a potential indigenous source for commercial PHA preparation.
Mitogenic potential of the partially purified lectin from P. vulgaris isolated by ammonium sulphate precipitation was assessed by lymphocyte transformation test (LTT) and was compared with three commercially available phytohemagglutinins (PHA). The blast inducing capacity and mitotic index analysis revealed that this preparation has potential to be used in the indigenous commercial production of PHA which is routinely used for human chromosomal studies from the peripheral blood culture at, at present, is imported.
Sengupta
S
S
Department of Genetics, Barkatullah University, Bhopal, India.
Sengupta
L K
LK
D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008940:Mitotic Index; D010835:Phytohemagglutinins / Q000302:isolation & purification*; D037121:Plant Lectins; D010946:Plants, Medicinal
D010835:Phytohemagglutinins; D037121:Plant Lectins