Some amines as inhibitors of pea diamine oxidase.
The inhibition of pea diamine oxidase (DAO; EC 1.4.3.6) by some amines and chelating agents has been studied. Ethylenediamine (competitive, Ki = 5.2 mmol.l-1), cis-1,2-diaminocyclohexane (noncompetitive, Ki = 2.9 mmol.l-1) and trans-1,2-diaminocyclohexane (competitive, Ki = 3.6 mmol.l-1) are weak inhibitors whereas diethylenetriamine (noncompetitive, Ki = 7.2 mumol.l-1), triethylenetetraamine (partial noncompetitive, Ki = 0.57 mumol.l-1) are potent inhibitors. The chelating agents 1,10-phenanthroline (Ki = 0.031 mmol.l-1), and 2,2'-bipyridyl (Ki = 0.058 mmol.l-1) are noncompetitive inhibitors. The pH-inhibition profile for the inhibitors indicates that the protonated forms are the active species.
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Department of Analytical and Organic Chemistry, Faculty of Science, Palacký University, Olomouc, Czechoslovakia.
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D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors*; D000588:Amines / Q000494:pharmacology*; D002151:Calorimetry; D002614:Chelating Agents / Q000494:pharmacology*; D007887:Fabaceae / Q000201:enzymology*; D007700:Kinetics; D015394:Molecular Structure; D010946:Plants, Medicinal; D013329:Structure-Activity Relationship
D000588:Amines; D002614:Chelating Agents; D006631:Amine Oxidase (Copper-Containing)