title: Demonstration of a neurokinin A receptor subtype in transfected fibroblasts.
abstract: In competitive radioligand binding assays, the NK2 receptor antagonists [Tyr5,D-Trp6,8,9,Arg10]NKA(4-10) (MEN 10207) and [Tyr5,D-Trp6,8,9,Arg10]NKA(3-10) (MEN 10208) had high and low affinity, respectively, in bovine stomach membranes and SKLKB82#3 cells, a murine fibroblast cell line transfected with a cDNA encoding for the bovine NK2 receptor. These antagonists also had different affinities when inhibiting neurokinin A-induced polyphosphoinositide hydrolysis in SKLKB82#3 murine fibroblasts. Thus, the de novo protein expressed by the SKLKB82#3 murine fibroblasts may represent a distinct NK2 receptor subtype.
authors.affiliation: Department of Pharmacology, University of Arizona, College of Medicine, Tucson 85724.
authors.forename: A K
authors.initials: AK
authors.lastname: Henderson
authors.forename: H I
authors.initials: HI
authors.lastname: Yamamura
authors.forename: C A
authors.initials: CA
authors.lastname: Maggi
authors.forename: S H
authors.initials: SH
authors.lastname: Buck
authors.forename: P L
authors.initials: PL
authors.lastname: Van Giersbergen
authors.forename: W R
authors.initials: WR
authors.lastname: Roeske
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D007457:Iodine Radioisotopes; D015288:Neurokinin A / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D011869:Radioligand Assay; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D014162:Transfection
chemical_list: D007457:Iodine Radioisotopes; D009842:Oligopeptides; D010446:Peptide Fragments; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter; C063615:neurokinin A (4-10), Tyr(5)-Trp(6,8,9)-Arg(10)-; C068553:MEN 10208; D015288:Neurokinin A