Transport parameter estimation from lymph measurements and the Patlak equation.
Two methods of estimating protein transport parameters for plasma-to-lymph transport data are presented. Both use IBM-compatible computers to obtain least-squares parameters for the solvent drag reflection coefficient and the permeability-surface area product using the Patlak equation. A matrix search approach is described, and the speed and convenience of this are compared with a commercially available gradient method. The results from both of these methods were different from those of a method reported by Reed, Townsley, and Taylor [Am. J. Physiol. 257 (Heart Circ. Physiol. 26): H1037-H1041, 1989]. It is shown that the Reed et al. method contains a systematic error. It is also shown that diffusion always plays an important role for transmembrane transport at the exit end of a membrane channel under all conditions of lymph flow rate and that the statement that diffusion becomes zero at high lymph flow rate depends on a mathematical definition of diffusion.
Watson
P D
PD
Department of Physiology, University of South Carolina, Columbia 29208.
Wolf
M B
MB
D000818:Animals; D001692:Biological Transport; D002196:Capillaries / Q000378:metabolism*; D004058:Diffusion; D006801:Humans; D008196:Lymph / Q000378:metabolism*; D008954:Models, Biological; D009994:Osmolar Concentration; D011506:Proteins / Q000378:metabolism*; D012984:Software
D011506:Proteins