Cytoprotective effect of dilazep on hydrogen peroxide-perturbed vascular endothelial cells.
The effect of dilazep and dimethyl thiourea (DMTU) on the hydrogen peroxide-derived injury of culture pulmonary artery epithelial cells (CPAEC) was assessed by colorimetric assay of MTT formazan (MTT formazan assay). When CPAEC were treated with hydrogen peroxide, neither cell lysis nor detachment of the cells from surface of the well was observed. However, the MTT formazan formation was decreased in a time and dose dependent manner. The decrease in the formation was significantly suppressed in the presence of dilazep (0.1 to 10 microM) or DMTU (0.01 to 0.3 microM). CPAEC treated with hydrogen peroxide in the same way enhanced an activation of prothrombin, and this enhancement was significantly inhibited in the presence of dilazep (1 to 3 microM). These data indicate that dilazep exerts a cytoprotective effect against challenges of intracellular oxidant produced by hydrogen peroxide and suppresses augmented procoagulant activity of injured cells.
Tanabe
S
S
Tokyo Research Laboratories, Kowa Company, Ltd., Japan.
Tamaki
T
T
Wada
Y
Y
Sumiyoshi
A
A
Funahara
Y
Y
D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003124:Colorimetry; D004109:Dilazep / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000293:injuries; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000009:adverse effects*; D000860:Hypoxia / Q000188:drug therapy / Q000378:metabolism; D011516:Prothrombin / Q000187:drug effects; D011651:Pulmonary Artery / Q000166:cytology*; D013778:Tetrazolium Salts / Q000032:analysis; D013844:Thiazoles / Q000032:analysis; D013890:Thiourea / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D013997:Time Factors
D013778:Tetrazolium Salts; D013844:Thiazoles; C038983:1,3-dimethylthiourea; D011516:Prothrombin; D006861:Hydrogen Peroxide; C022616:thiazolyl blue; D004109:Dilazep; D013890:Thiourea