title: Contribution of single genes within the unique short region of Aujeszky's disease virus (suid herpesvirus type 1) to virulence, pathogenesis and immunogenicity.
abstract: Pigs (3 and 10 weeks old) were infected intranasally with Aujeszky's disease virus (ADV) mutants that functionally lacked one of the non-essential genes in the unique short region of the genome (except the gene encoding the 11K protein). Virus excretion in oropharyngeal fluid and disease symptoms were monitored. Some pigs were killed to study pathogenesis, whereas others were challenged with virulent ADV 8 weeks after the primary infection. Mutants lacking protein kinase, or glycoproteins gp63 or gI showed reduced virulence, but mutants lacking gX or the 28K protein showed normal virulence. Glycoprotein gI appears to affect the tissue tropism of ADV in pigs, presumably by facilitating the spread of the virus through the central nervous system. In this study, there was no correlation between virulence and virus multiplication in either cultured cells or in the oropharynx in vivo. All mutants induced neutralizing antibody and complete or partial protection against challenge infection. Complete protection was obtained by inoculation with the gI and gX mutants, whereas incomplete protection was obtained using gp63 and protein kinase mutants. Complete clinical and virological protection was associated with the absence of secondary antibody responses in the serum.
authors.affiliation: Central Veterinary Institute, Department of Virology, Lelystad, The Netherlands.
authors.forename: T G
authors.initials: TG
authors.lastname: Kimman
authors.forename: N
authors.initials: N
authors.lastname: de Wind
authors.forename: N
authors.initials: N
authors.lastname: Oei-Lie
authors.forename: J M
authors.initials: JM
authors.lastname: Pol
authors.forename: A J
authors.initials: AJ
authors.lastname: Berns
authors.forename: A L
authors.initials: AL
authors.lastname: Gielkens
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001921:Brain / Q000382:microbiology; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D016254:Mutagenesis, Insertional; D009305:Nasopharynx / Q000382:microbiology; D011557:Pseudorabies / Q000209:etiology / Q000276:immunology / Q000382:microbiology*; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000209:etiology / Q000276:immunology / Q000382:microbiology*; D014774:Virulence / Q000235:genetics
chemical_list: D000914:Antibodies, Viral