[Comparative antigen analysis of Trichomonas vaginalis by enzyme-linked immunoelectrotransfer blot technique].
Analysis of six isolates of Trichomonas vaginalis was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunoelectrotransfer blot (EITB). Trichloroacetic acid-treated antigens of the 6 isolates revealed 25 protein profiles ranging 12-170 kDa of molecular weight in SDS-PAGE. In EITB, the specific immunogenic bands were visualized at 51 kDa and 96 kDa when HY-1 antigen was probed with different mice sera immunized with 6 isolates of T. vaginalis. The banding patterns with different sera showed isolate-to-isolate variability. In EITB, homologous antigen (HY-1) did not show any enhanced response in reacting to homologous antiserum (HY-1) when 6 isolates of T. vaginalis were probed with a single serum (HY-1). It is assumed that the different banding patterns of six isolates show isolate-to-isolate variability and immunogenic common bands in 41, 47, 74 and 94 kDa on EITB may connote the important significance on immune response in T. vaginalis infection.
Min
D Y
DY
Department of Parasitology, College of Medicine, Hanyang University, Seoul, Korea.
Leem
M H
MH
Kim
J M
JM
Choi
Y K
YK
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D051379:Mice; D008970:Molecular Weight; D014245:Trichomonas Infections / Q000276:immunology; D014246:Trichomonas vaginalis / Q000276:immunology*
D000953:Antigens, Protozoan