The effect of immune mediators (cytokines) on the release of endothelium-derived relaxing factor (EDRF) and of prostacyclin by freshly harvested endothelial cells.
The effect of recombinant tumor necrosis factor and other cytokines stimulated by LPS (lipopolysaccharide), on the release of endothelial-derived relaxing factor and of prostacyclin was investigated using freshly harvested endothelial cells attached to plastic microcarrier beads. The results show that the cytokines failed to interfere with the release of EDRF and prostacyclin under the conditions of these experiments.
Dudek
R
R
Huntington Medical Research Institutes, Department of Experimental Cardiology, Pasadena, CA 91105.
Kibira
S
S
Kähler
J
J
Bing
R J
RJ
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D001769:Blood / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000648:ultrastructure; D011464:Epoprostenol / Q000378:metabolism*; D008070:Lipopolysaccharides; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009569:Nitric Oxide / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology
D016207:Cytokines; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D009569:Nitric Oxide; D000001:Calcimycin; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol; D006152:Cyclic GMP