[Elevated production of GM-CSF by peripheral blood mononuclear cells stimulated with mite antigens in children with bronchial asthma].
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine which can stimulate the proliferation and functions of eosinophils and neutrophils. We have studied whether peripheral blood mononuclear cells (PBMC), obtained from children with stable asthma, can produce GM-CSF through stimulation with crude Dermatophagoides farinae (Df) antigen in vitro. The patients had strongly positive RAST results for house dust mite Df. Levels of GM-CSF in PBMC culture supernatant were assayed by an enzyme immunoassay. When stimulated with Df antigen, patient's PBMC released significantly increased levels of GM-CSF compared with unstimulated ones. PBMC of non-allergic controls, however, did not. The addition of polymyxin B, an inactivator of endotoxin, did not suppress the Df-antigen-induced production of GM-CSF. Levels of GM-CSF produced by PBMC stimulated with Df antigens were significantly higher in the patients than in the controls. These results indicate that PBMC of children with Df-allergic asthma, unlike those of non-allergic children, can produce elevated levels of GM-CSF by stimulation with Df antigens.
Ishiguro
A
A
Department of Pediatrics, Mizonokuchi Hospital, Teikyo University School of Medicine.
Shimbo
T
T
Takahashi
H
H
Fukushima
K
K
Hatano
M
M
Nakahata
T
T
Komiyama
A
A
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001249:Asthma / Q000097:blood*; D002648:Child; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008925:Mites / Q000276:immunology*
D000941:Antigens; D016178:Granulocyte-Macrophage Colony-Stimulating Factor