title: Comparison of the X-ray structure of native rubredoxin from Pyrococcus furiosus with the NMR structure of the zinc-substituted protein.
abstract: The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at -161 degrees C, and the NMR structure of the zinc-substituted protein, determined in solution at 45 degrees C, are compared. The NMR and X-ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hyperthermostability.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland Baltimore County 21228.
authors.forename: P R
authors.initials: PR
authors.lastname: Blake
authors.forename: M W
authors.initials: MW
authors.lastname: Day
authors.forename: B T
authors.initials: BT
authors.lastname: Hsu
authors.forename: L
authors.initials: L
authors.lastname: Joshua-Tor
authors.forename: J B
authors.initials: JB
authors.lastname: Park
authors.forename: D R
authors.initials: DR
authors.lastname: Hare
authors.forename: M W
authors.initials: MW
authors.lastname: Adams
authors.forename: D C
authors.initials: DC
authors.lastname: Rees
authors.forename: M F
authors.initials: MF
authors.lastname: Summers
mesh_terms: D000595:Amino Acid Sequence; D001105:Archaea / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D012416:Rubredoxins / Q000737:chemistry* / Q000378:metabolism*; D014961:X-Ray Diffraction / Q000379:methods; D015032:Zinc / Q000378:metabolism*
chemical_list: D012416:Rubredoxins; D015032:Zinc