A rapid assay for the measurement of Na+,K(+)-ATPase inhibitors.
A rapid and automated assay for inhibitors of Na+, K(+)-ATPase was developed by determining the amount of inorganic phosphorus (Pi) released by Na+,K(+)-ATPase in a centrifugal analyzer. This method avoids long incubation, strong acids and centrifugation as in the conventional manual method. The coefficients of variation of intra- and inter-assay at ouabain concentration 0.5 mumol/L were 1.0 and 1.4%, respectively. The method is quick, reproducible and easy compared with current techniques.
Chan
E L
EL
Department of Chemical Pathology, Chinese University of Hong Kong, Shatin.
Swaminathan
R
R
D000818:Animals; D002498:Centrifugation; D002624:Chemistry, Clinical / Q000379:methods; D004285:Dogs; D005312:Fetal Blood; D006801:Humans; D007668:Kidney / Q000201:enzymology* / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D010710:Phosphates / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013662:Tea; D000097795:ATPase Inhibitory Protein
D010042:Ouabain; D010710:Phosphates; D011506:Proteins; D000254:Sodium-Potassium-Exchanging ATPase; D013662:Tea; D000097795:ATPase Inhibitory Protein