[Identification of mycoplasma by the growth inhibition of samples isolated from cell cultures].
Cell cultures must be continuously screened for the presence of mycoplasma because, although these microorganisms sometimes pass unnoticed, they may cause chromosomic alterations and interfere with viral replication, antibody and interferon production etc. The International Organization for Mycoplasmology (IOM) recommends the isolation and identification of mycoplasma with a view to the detection of the origin of the infection and the improvement of the quality of the cultures. In this paper, 37 samples belonging to 27 cell lines contaminated with mycoplasma were assayed by the growth inhibition test. It is known that Mycoplasma orale is the most common human mycoplasma contaminant of cell cultures, the major vehicle of contamination being mouth pippeting, while commercial bovine serum in the main source for Mycoplasma arginini and Acholeplasma laidlawii. M. arginini was found in 18 (48.65%) of the cell samples tested, A. laidlawii in 15 (40.55%), and M. orale in two (5.40%). Two other samples could not be identified by the antisera used (antisera against M. arginini, M. orale, Mycoplasma hyorhinis and A. laidlawii) their characteristics being "fried egg" colonies, digitonine sensitivity, Dienes stained, positive glucose catabolism, negative arginini hydrolysis, and negative tetrazolium reduction. No more than one type of mycoplasma was found in each cell culture.(ABSTRACT TRUNCATED AT 250 WORDS)
Timenetsky
J
J
Departamento de Microbiologia, Instituto de Ciências Biomédicas da Universidade de São Paulo, Brasil.
Miyaki
C
C
Mendes
I F
IF
de Rizzo
E
E
D001431:Bacteriological Techniques; D002478:Cells, Cultured / Q000382:microbiology; D003470:Culture Media; D009174:Mycoplasma / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media
Identificação de microplasmas pela inibição de crescimento de amostras isoladas de culturas celulares.