title: The production of hybridoma cell line secreting monoclonal antibodies against Xanthomonas campestris pv. oryzae and its application in the classification of strains.
abstract: By the fusion of mouse myeloma cells (SP2/0-Ag14) and spleen cells derived from BALB/c mice immunized with the preparation of Xanthomonas campestris pv. oryzae Ks-6-6, Os-213, Yz-32 and Yz-24, we obtained 12 hybridoma cell lines secreting monoclonal antibodies. None of the McAbs cross-reacted with the other varieties of plant pathogenetic and non-pathogenetic bacteria. The McAbs could distinguish three variant serotypes of strains. Antibody titers of ascites were about 1:10(3)-1:10(6) when measured by ELISA method. The McAbs could differentiate 6 epitopes. Based on the epitopes, the 63 strains of X. campestris pv. oryzae we collected were grouped into nine groups.
authors.affiliation: Department of Plant Protection, Jiangsu Agricultural College, China.
authors.forename: B
authors.initials: B
authors.lastname: Huang
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.forename: G
authors.initials: G
authors.lastname: Hu
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.forename: S
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000942:Antigens, Bacterial; D015373:Bacterial Typing Techniques; D001709:Biotechnology; D003429:Cross Reactions; D000939:Epitopes; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D016959:Xanthomonas campestris / Q000145:classification* / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes