Evaluation of antioxidant treatment with superoxide dismutase in rat liver transplantation after warm ischemia.
In order to investigate the effects of the exogenously administered radical scavenger superoxide dismutase (SOD) on the orthotopic liver graft, livers from male Wistar rats were transplanted after subjection to 40 min of warm ischemia and 30 min of storage at 4 degrees C. SOD was given at the onset of ischemia and before reperfusion as a supplement (6,000 IU) to the washout solutions. 30,000 IU were infused into the recipient. SOD reduced tissue levels of thiobarbituric acid-reacting substances at the end of ischemia (737 vs. 956 nmol/g; p < 0.01) and 60 min after the onset of reperfusion (629 vs. 947 nmol/g; p < 0.001) and preserved total adenine nucleotides after reperfusion (11.69 vs. 10.40 mumol/g; p < 0.01). Survival 2 weeks after transplantation was 18% (2/11) in the SOD group versus 10% (1/10; nonsignificant) in untreated animals. It is concluded that SOD protects the ischemically altered liver from radical mediated peroxidation and preserves hepatic energy stores upon reperfusion. However, in our model no major improvement in organ viability could by achieved.
Minor
T
T
Institute of Experimental Medicine, University of Cologne, FRG.
Chung
C W
CW
Yamamoto
Y
Y
Obara
M
M
Saad
S
S
Isselhard
W
W
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D005609:Free Radicals; D006085:Graft Survival / Q000187:drug effects; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control; D013482:Superoxide Dismutase / Q000494:pharmacology*
D000227:Adenine Nucleotides; D005609:Free Radicals; D013482:Superoxide Dismutase