A statistical approach to the ecology of Porphyromonas gingivalis.
Previous studies have suggested that infections with Porphyromonas gingivalis, associated with periodontal disease, may consist of one clonal type. It has also been shown that each individual patient carries a unique clonal type of P. gingivalis, as assessed by DNA fingerprinting. This issue was further examined by random collection of multiple isolates of P. gingivalis from multiple sites in several patients, and characterization of these isolates by DNA fingerprinting. Although most patients appeared to be infected exclusively by one clonal type of P. gingivalis, at least one patient was found to harbor two distinct clonal types. This indicates that the simultaneous presence of different clonal types of P. gingivalis can occur. A statistical method was developed for retrospective analysis of these data for estimation of whether the remainder of these patients were actually infected with single or multiple clonal types of P. gingivalis. With this statistical method, a confidence interval was calculated for estimation of the true proportion of a single observed clonal type in the potential population of P. gingivalis that might be recovered from an infected patient. Statistically, the sampling of small numbers of sites per patient or isolates per site leads to a wide confidence interval for the estimated true proportion of the observed clonal type in the infecting P. gingivalis population. For example, when five sites in an oral cavity yield indistinguishable P. gingivalis isolates, then the true proportion of this clonal type in the total P. gingivalis population in the infected oral cavity is estimated to be in the interval between 55% and 100% (at a 95% confidence level).(ABSTRACT TRUNCATED AT 250 WORDS)
Loos
B G
BG
Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo 14214.
Van Winkelhoff
A J
AJ
Dunford
R G
RG
Genco
R J
RJ
De Graaff
J
J
Dickinson
D P
DP
Dyer
D W
DW
D016172:DNA Fingerprinting; D004463:Ecology; D005881:Gingiva / Q000382:microbiology; D006801:Humans; D015233:Models, Statistical; D014066:Palatine Tonsil / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D012189:Retrospective Studies; D012463:Saliva / Q000382:microbiology; D014059:Tongue / Q000382:microbiology