Secretion of peptidylglycine alpha-amidating monooxygenase (PAM) from rat salivary glands.
Peptidylglycine alpha-amidating monooxygenase (PAM) is a regulating enzyme to synthesize the biologically active hormones having carboxy-terminal amide. In the present study we investigated secretion of the enzyme from rat saliva. Property of PAM in the saliva was similar to that in the submandibular gland. Both enzymes showed similar pH optimum at 5.0 and optimal ascorbic acid concentration at 2.5 mM. But molecular size of PAM in the saliva was 75 kDa in the gel permeation chromatography on Superose 12 column, while the size in the submandibular gland was 25 kDa. After the treatment with trypsin, PAM in the saliva was converted to a small size molecule, which is similar to the size in rat submandibular gland. These and other data indicate that a native molecular size of PAM is secreted into saliva and plays some physiological roles.
Kato
T
T
Laboratory of Molecular Recognition, Graduate School of Integrated Science, Yokohama City University, Japan.
Hanaoka
K
K
Ochiai
O
O
Chikuma
T
T
Takeda
K
K
Yajima
R
R
Kumegawa
M
M
D000375:Aging / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D006899:Mixed Function Oxygenases / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes; D009842:Oligopeptides / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012463:Saliva / Q000201:enzymology*; D013361:Sublingual Gland / Q000201:enzymology* / Q000254:growth & development; D013363:Submandibular Gland / Q000201:enzymology* / Q000254:growth & development
D009097:Multienzyme Complexes; D009842:Oligopeptides; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase