title: Cloning and sequence of Bacillus subtilis purA and guaA, involved in the conversion of IMP to AMP and GMP.
abstract: Bacillus subtilis genes purA, encoding adenylosuccinate synthetase, and guaA, coding for GMP synthetase, appear to be lethal when cloned in multicopy plasmids in Escherichia coli. The nucleotide sequences of purA and guaA were determined from a series of gene fragments isolated by polymerase chain reaction amplification, library screening, and plasmid rescue techniques. Identifications were based on amino acid sequence alignments with enzymes from other organisms. Comparison of the 5'-flanking regions of purA and guaA with the pur operon suggests similarities in mechanisms for gene regulation. Nucleotide sequences are now available for all genes involved in the 14-step pathway for de novo purine nucleotide synthesis in B. subtilis.
authors.affiliation: Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907-1153.
authors.forename: P
authors.initials: P
authors.lastname: Mäntsälä
authors.forename: H
authors.initials: H
authors.lastname: Zalkin
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism; D000265:Adenylosuccinate Synthase / Q000235:genetics*; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D019731:Carbon-Nitrogen Ligases; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006157:Guanosine Monophosphate / Q000378:metabolism; D007291:Inosine Monophosphate / Q000378:metabolism; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D007291:Inosine Monophosphate; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate; D008025:Ligases; D019731:Carbon-Nitrogen Ligases; D000265:Adenylosuccinate Synthase; C020828:GMP synthase (glutamine-hydrolyzing)