Effect of D2O on maize plasmalemma ATPase and electron transport coupled proton pumping.
Heavy water (D2O) has been used as a putative inhibitor of the plasma membrane H(+)-ATPase and the plasma membrane redox system. Concentrations above 50% D2O inhibited H+ secretion and the plasma membrane redox system of Zea mays L. roots. Inhibition of H+ secretion by vanadate was reduced in presence of D2O. The plasma membrane of roots was transiently depolarized after the addition of heavy water in concentrations above 5%. The repolarization of the plasma membrane that takes place while the H+ secretion is still reduced by heavy water indicates that, despite the overall inhibiting effect of D2O, the plant is still able to regulate the membrane potential.
Döring
O
O
Universität Hamburg, Institut für Allgemeine Botanik, FRG.
Böttger
M
M
D002462:Cell Membrane / Q000201:enzymology / Q000502:physiology; D003903:Deuterium / Q000494:pharmacology*; D017666:Deuterium Oxide; D004579:Electron Transport / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D010084:Oxidation-Reduction; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D014638:Vanadates / Q000494:pharmacology; D014867:Water / Q000494:pharmacology*; D003313:Zea mays / Q000201:enzymology*
D014867:Water; D014638:Vanadates; D003903:Deuterium; D006180:Proton-Translocating ATPases; D017666:Deuterium Oxide