A basic study on the hybridoma cell culture of microencapsulation.
We report here that the constitute component of Na alginate is a very important factor on microcapsular strength. We developed a method of estimating viable cell count in batch microcapsular process and the factors influencing the full loading rate in microcapsules. The physical integrity of microcapsule and full loading rate were greater than 95%. The cell line of C3 hybridoma was found to reach maximum intracapsule cell density of 8.8 x 10(6) - -2.1 x 10(7) cells/ml during static culture period of 6-10 days. During this period, the mouse monoclonal antibody accumulated in the intracapsular space to a final concentration of 379 micrograms/ml. Analysis of intracapsular protein by SDS gel electrophoresis during the culture period demonstrated that there was one band or two bands of protein which means its purity is relatively high.
Ning
G
G
Department of Radiation Medicine, Second Military Medical University, Shanghai, China.
Qian
J
J
Guo
M
M
D000464:Alginates; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001709:Biotechnology; D002214:Capsules; D002452:Cell Count; D002455:Cell Division; D002470:Cell Survival; D003584:Cytological Techniques; D005069:Evaluation Studies as Topic; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D051379:Mice
D000464:Alginates; D000911:Antibodies, Monoclonal; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid