The multifunctional folic acid synthesis fas gene of Pneumocystis carinii appears to encode dihydropteroate synthase and hydroxymethyldihydropterin pyrophosphokinase.
We describe the cloning of a multifunctional folic acid synthesis (fas) gene from Pneumocystis carinii. The nucleotide sequence contains an open reading frame interrupted by three introns, that encodes a protein of 740 amino acids with an Mr of 97,278. The predicted Fas protein has homology to two enzyme domains, dihydropteroate synthase and 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, both of which are involved in folate synthesis, and at least one other region of unknown function.
Volpe
F
F
Department of Molecular Sciences, Wellcome Research Laboratories, Kent, U.K.
Dyer
M
M
Scaife
J G
JG
Darby
G
G
Stammers
D K
DK
Delves
C J
CJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004094:Dihydropteroate Synthase / Q000235:genetics*; D017854:Diphosphotransferases; D005492:Folic Acid / Q000096:biosynthesis*; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000235:genetics*; D011010:Pneumocystis / Q000201:enzymology* / Q000235:genetics
D005492:Folic Acid; D004094:Dihydropteroate Synthase; D010770:Phosphotransferases; D017854:Diphosphotransferases; C019684:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase