Prolactin isoforms secreted by human prolactinomas.
Prolactin (hPRL) secreted by human prolactinoma cells in culture was purified by gel filtration, lectin affinity chromatography and gel electrophoresis in order to identify the different isoforms of the hormone and to test their respective immunoreactivities and bioactivities. The nonglycosylated hPRL (NG-hPRL), unbound to lectins, was the major form and was a species (NG1-hPRL), of 23,000 (M(r)) apparent molecular weight. The lectin-bound glycosylated hPRL (G-hPRL) consisted of three forms, G1-, G2- and G3-hPRL, of identical molecular weights (25,000 M(r)). Endoglycosidase treatment indicated that these three forms differed by the heterogeneity of their carbohydrate chains. These G-PRLs proved to be 68% less immunoreactive and 50% less bioactive than NG-hPRL. It is concluded from these data that, in prolactinomas, the main variant of the hormone is the nonglycosylated form of PRL.
Hoffmann
T
T
Laboratoire de Neuroendocrinologie Expérimentale, INSERM U 297, Marseille, France.
Gunz
G
G
Brue
T
T
Jaquet
P
P
Ronin
C
C
D000911:Antibodies, Monoclonal; D002455:Cell Division / Q000187:drug effects; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D015592:Immunoradiometric Assay; D007525:Isoelectric Focusing; D008223:Lymphoma; D008970:Molecular Weight; D010911:Pituitary Neoplasms / Q000378:metabolism*; D011388:Prolactin / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D015175:Prolactinoma / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D011388:Prolactin; D006596:Hexosaminidases