title: Activin receptor mRNA expression in rat testicular cell types.
abstract: cDNA encoding the extracellular domain of the rat activin receptor was cloned using the polymerase chain reaction (PCR). This cDNA is highly homologous to cDNA encoding the extracellular domain of the mouse activin receptor, whereas at the protein level the extracellular domains of both receptors are identical. Employing this cDNA as a probe in Northern blot analysis, expression of two activin receptor mRNAs (6 kb and 4 kb) was observed, in testes of immature and mature rats. Between day 21 and 28 of postnatal development, a large increase in testicular expression of the 4 kb mRNA was found, suggesting expression of this activin receptor mRNA in germ cells. The 4 kb mRNA was indeed present in isolated pachytene spermatocytes and round spermatids, but was absent in elongating spermatids. Sertoli cells obtained from immature and mature rats expressed both the 6 kb and 4 kb mRNAs, whereas the expression of these mRNAs in Leydig cell preparations was very low. These results may imply that activin has multiple actions in the control of testicular function.
authors.affiliation: Department of Endocrinology and Reproduction, Medical Faculty, Erasmus University Rotterdam, Netherlands.
authors.forename: J P
authors.initials: JP
authors.lastname: de Winter
authors.forename: A P
authors.initials: AP
authors.lastname: Themmen
authors.forename: J W
authors.initials: JW
authors.lastname: Hoogerbrugge
authors.forename: I A
authors.initials: IA
authors.lastname: Klaij
authors.forename: J A
authors.initials: JA
authors.lastname: Grootegoed
authors.forename: F H
authors.initials: FH
authors.lastname: de Jong
mesh_terms: D029404:Activin Receptors; D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000235:genetics*; D013737:Testis / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D004247:DNA; D029404:Activin Receptors