Perfluorooctanoic acid has persistent effects on peroxisome proliferation and related parameters in mouse liver.
Male C57Bl/6 mice were treated for 5 days with 0.05% perfluorooctanoic acid (PFOA) in their diet. This treatment resulted in a potent induction of peroxisomal fatty acid beta-oxidation in the liver. In order to investigate recovery from treatment with PFOA, mice were given normal laboratory chow for up to 20 days after termination of PFOA administration. It was established that the activities of peroxisomal lauoryl-CoA oxidase and palmitoyl-CoA oxidation were still elevated 2-3 weeks after termination of treatment. The catalase activity recovered in the cytosolic fraction was also still significantly elevated after 20 days with normal laboratory chow. Furthermore, the protein content of the mitochondrial fraction was increased by PFOA and had not returned to control level at the end of the recovery period. Perfluorooctanoic acid also caused a persistent effect in omega hydroxylation of lauric acid (cytochrome P-452). The activities of cytosolic DT-diaphorase and glutathione transferase were also enhanced by PFOA. However, these two enzymes recovered relatively rapidly from the treatment (2-20 days). This study reveals two different patterns of recovery from PFOA treatment, one involving parameters that recovered completely, or almost completely, from PFOA treatment after 20 days and another involving parameters that were still elevated at the end of the recovery period.
Sohlenius
A K
AK
Department of Biochemistry, Wallenberg Laboratory, Stockholm University, Sweden.
Lundgren
B
B
DePierre
J W
JW
D042962:Acyl-CoA Oxidase; D000818:Animals; D002210:Caprylates / Q000494:pharmacology*; D002374:Catalase / Q000187:drug effects; D004032:Diet; D005466:Fluorocarbons / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008830:Microbodies / Q000187:drug effects* / Q000201:enzymology; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000187:drug effects; D010088:Oxidoreductases / Q000187:drug effects; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology
D002210:Caprylates; D005466:Fluorocarbons; C023036:perfluorooctanoic acid; D010088:Oxidoreductases; D002374:Catalase; D042962:Acyl-CoA Oxidase; D016660:NAD(P)H Dehydrogenase (Quinone); D005982:Glutathione Transferase