title: [Determination of scopolia extract in commercial gastrointestinal drugs by high-performance liquid chromatography].
abstract: Tropane alkaloids, contained in gastrointestinal drugs including Scopolia Extract, were determined using high-performance liquid chromatographic method. Tropane alkaloids in commercial drugs were extracted with 0.5 N hydrochloric acid test solution. This solution was adjusted to pH 10.0 with a strong ammonia solution, extracted with ethyl ether, and separated on TSKgel ODS-120T (4.6 mm i.d. x 15 cm; temperature, 30 degrees C; flow rate, 1.5 ml/min; detector, UV 210 nm) with a mixture of 1/15 M sodium phosphate buffer (pH 2.5) and acetonitrile (68:32) containing 12 mM sodium dodecyl sulfate. The contents of atropine in the gastrointestinal drugs were 0.25-0.34 mg (coefficient of variation = 0.20-2.61%) a day.
authors.affiliation: Central Research Laboratory, SS Pharmaceutical Co. Ltd., Chiba, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakanishi
authors.forename: K
authors.initials: K
authors.lastname: Imamori
authors.forename: A
authors.initials: A
authors.lastname: Iwasa
mesh_terms: D000863:Antacids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004304:Dosage Forms; D014326:Tropanes / Q000032:analysis*
chemical_list: D000863:Antacids; D004304:Dosage Forms; D014326:Tropanes