Developmental changes in erythropoietin receptor expression of fetal mouse liver.
Erythropoietin (EPO) stimulates proliferation and differentiation of late erythroid precursor cells (CFU-E) and thereby determines the rate of erythropoiesis. Liver is the major erythropoietic site in a fetus. We dealt with developmental changes in CFU-E and EPO receptor (EPO-R) of fetal mouse liver. The affinity of the EPO-R to EPO was unchanged during fetal development. The population size of CFU-E, the number of EPO-R per liver cell, and EPO-R mRNA decreased as gestation proceeded, in a pattern indicating that the expression of EPO-R on erythroid precursor cells in fetal mouse liver is governed mostly by the process of mRNA production.
Masuda
S
S
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
Hisada
Y
Y
Sasaki
R
R
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D004921:Erythropoietin / Q000378:metabolism*; D008099:Liver / Q000196:embryology / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins; D004921:Erythropoietin