Regulation of plasminogen activation in rat cell lines.
The regulation of plasminogen activators (PA) and their inhibitors (PAI) in the rat cell lines: HTC and L2 was studied. HTC plasminogen activator inhibitor type 1 (PAI-1) production was stimulated by dexamethasone, serum factors and insulin; that of tissue-type plasminogen activator (tPA) by cAMP raising agents. Retinoic acid, butyrate, phorbol ester and endotoxin did not affect net PA/PAI activity elaborated by HTC. L2 cells produced tPA, which production was stimulated by retinoic acid, phorbol myristate acetate, butyrate and cAMP; serum factors blunted their response, whereas in the synthetic serum substituting medium Ultraculture and with cocktail Ultroser the action of tPA stimulators was enhanced.
Reinders
J H
JH
Solvay Duphar B.V., Department of Vascular Pharmacology, DA Weesp, The Netherlands.
Kaczmarek
P
P
van Giezen
J J
JJ
Jansen
J W
JW
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology; D004407:Dysgerminoma / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007328:Insulin / Q000494:pharmacology; D008113:Liver Neoplasms / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism*; D051381:Rats; D010959:Tissue Plasminogen Activator / Q000302:isolation & purification / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007328:Insulin; D017395:Plasminogen Activator Inhibitor 1; D014212:Tretinoin; D003994:Bucladesine; D003907:Dexamethasone; D000242:Cyclic AMP; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator; D015056:1-Methyl-3-isobutylxanthine