Monoclonal antibodies against human fibroblast collagenase and the design of an enzyme-linked immunosorbent assay to measure total collagenase.
Monoclonal antibodies have been raised against purified human fibroblast collagenase and characterised. One of these antibodies has been used in combination with a polyclonal anticollagenase antibody in a double antibody sandwich ELISA to measure collagenase. The assay range was 2-50 ng/ml collagenase. The assay measures total collagenase, i.e. pro- and active enzyme as well as collagenase in complex with TIMP. The level of collagenase has been measured in sera samples from patients with rheumatoid arthritis and compared with age- and sex-matched controls. The levels measured were: rheumatoid arthritis, 69 +/- 29 ng/ml; normal, 49 +/- 14 ng/ml.
Clark
I M
IM
Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge, UK.
Powell
L K
LK
Wright
J K
JK
Cawston
T E
TE
Hazleman
B L
BL
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D001826:Body Fluids / Q000201:enzymology; D017364:Collagenases / Q000032:analysis* / Q000276:immunology; D004792:Enzyme Precursors / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans
D000911:Antibodies, Monoclonal; D004792:Enzyme Precursors; D017364:Collagenases; C022440:procollagenase