[The application of monoclonal antibodies (McAbs) against human serum IgA in single radial immunodiffusion (SRID)].
Among many McAbs to human IgA, two McAbs (A1A10 and A1C9) against two determinants were selected and mixed. They formed opaque precipitation rings with IgA in agarose containing 2% PEG 6000. SRID with our mixed McAbs or PcAbs was used to determine serum IgA concentration in 40 undiluted serum specimens: a comparison of the two showed similar results, with a correlation coefficient of 0.9493 (P < 0.001). We conclude that mixed McAbs A1A10 and A1C9 can replace PcAbs in SRID for the measurement of human total serum IgA.
Zou
M
M
Institute of Basic Medical Sciences, Beijing.
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006801:Humans; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007070:Immunoglobulin A