Impact of serum concentration of the medium and fasting on the imprintability of the insulin receptors of Chang liver cells.
When the cells of the Chang cell line came into interaction with a hormone (insulin) an imprinting-like phenomenon took place. The binding capacity of the receptors strengthened and this feature was transmitted to the descendant generations. The quality of the nutrient medium influenced the development of imprinting, when the cells were maintained in a medium containing 2% serum it was more difficult to evoke imprinting than in case the cells were kept in a medium containing 10% serum. If the cells were cultured kept in Tyrode (physiological) solution for 24 hours the possibility to evoke imprinting was lost. Difference could be observed between the behaviour of receptors in nuclear membrane and that of receptors in the plasma membrane; i.e. changes were more dynamic in the plasma membrane.
Csaba
G
G
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Hegyesi
H
H
Török
O
O
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003470:Culture Media; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D010949:Plasma; D011972:Receptor, Insulin / Q000378:metabolism*; D013194:Staining and Labeling
D003470:Culture Media; D007328:Insulin; D011972:Receptor, Insulin; D016650:Fluorescein-5-isothiocyanate