Production and characterization of two monoclonal antibodies to human glutathione peroxidase.
Glutathione peroxidase (GSH-Px) is an important selenium-containing enzyme which protects cells from oxidative damage. Two hybridoma clones (GPX-121 and GPX-347), producing mouse IgG1 monoclonal antibodies specific for GSH-Px, were established. Immunoblot analysis revealed that GPX-347 was specific for human GSH-Px, while GPX-121 cross-reacted with human, rat, mouse and rabbit GSH-Px. Correlation between GSH-Px content and its enzymatic activity was investigated in erythrocytes of 76 humans and in human lung adenocarcinoma PC-9 cells by using a sandwich type ELISA. The results indicated that GSH-Px activity was expressed higher than expected from GSH-Px content especially in the range of low GSH-Px concentration. PC-9 cells selenium depleted medium did not stain but the cytoplasm of PC-9 cells grown in medium supplemented with selenium stained strongly.
Suemizu
H
H
Department of Molecular Life Science (Cell Biology), Tokai University School of Medicine, Isehara-city, Japan.
Yoshimura
S
S
Tada
N
N
Watanabe
K
K
Moriuchi
T
T
D000230:Adenocarcinoma / Q000201:enzymology; D000328:Adult; D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D008175:Lung Neoplasms / Q000201:enzymology; D008297:Male; D008322:Mammals / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000276:immunology; D012643:Selenium / Q000494:pharmacology; D013045:Species Specificity; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007074:Immunoglobulin G; D009363:Neoplasm Proteins; D005979:Glutathione Peroxidase; D012643:Selenium