Finite life span of hybrids formed by fusion of different simian virus 40-immortalized human cell lines.
Simian virus 40 (SV40) genes are able to induce immortalization of normal human cells after a culture crisis during which unknown cellular genetic changes presumably occur. To determine whether these genetic changes are always identical, we performed somatic cell hybridization analysis of an SV40-immortalized human bronchial epithelial cell line, BET-1A. Fusion of BET-1A with an SV40-immortalized fibroblast cell line resulted in hybrids that senesced, indicating that these cell lines are in different complementation groups for immortalization.
Whitaker
N J
NJ
Children's Medical Research Foundation, Camperdown, New South Wales, Australia.
Kidston
E L
EL
Reddel
R R
RR
D000957:Antigens, Viral, Tumor / Q000032:analysis; D002459:Cell Fusion; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D005816:Genetic Complementation Test; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology*; D013539:Simian virus 40 / Q000235:genetics*; D013997:Time Factors
D000957:Antigens, Viral, Tumor