[A highly sensitive non-isotopic system of DNA hybridization using amplification (PCR) for identifying and indicating presence of Brucella].
A non-isotopic amplification system was used to identify and indicate Brucella. The terminal sequences of a protein gene fragment in Brucella outer membrane were identified and direct and reverse primers were chosen for a polymerase chain reaction. (PCR). PCR amplifies a specific DNA fragment, 700 kb in size, only in representatives of the Brucella genus. A probe was design, which is the central part of the amplified DNA fragment, 550 kb in size. Single Brucella cells were detectable with an unlabelled probe in the analyzed samples during hybridization reactions. The system can be recommended for a rapid and reliable analysis in medical and veterinary practice.
Kulakov
Iu K
IuK
Gorelov
V N
VN
Motin
V L
VL
Brukhanskiĭ
G V
GV
Skavronskaia
A G
AG
D001483:Base Sequence; D002002:Brucella / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D004269:DNA, Bacterial
Vysokochuvstvitel'naia neizotopnaia sistema gibridizatsii DNK s primeneniem amplifikatsii (PTsR) dlia identifikatsii i indikatsii brutsell.