Results of the DGTI workshop on the evaluation of the reactivity of monoclonal anti-D.
All D+ samples were detected by all monoclonal antibodies (mabs) without any problem. Very few of the 3,000 D- samples showed a +/- to ++ reaction with some of the IgG type mabs using the Coombs technique. It is still open whether these are specific reactions with weakly positive samples, which were negative with polyclonal sera. IgM type mabs detected up to 100% of the Du samples dependent on the Rh phenotype and the technique used (CCDuee > CcDuee > ccDuEe). Most of the CCDuee samples reacted very strongly and should no longer be regarded as Du.
Sonneborn
H H
HH
Biotest AG, Research Department, Dreieich, FRG.
Darda
C
C
Ernst
M
M
Flegel
W A
WA
Helmbold
W
W
Kasulke
D
D
Kühnl
P
P
Mueller-Eckhardt
C
C
Northoff
H
H
Poschmann
A
A
D000911:Antibodies, Monoclonal; D001788:Blood Grouping and Crossmatching; D001803:Blood Transfusion; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007518:Isoantibodies / Q000032:analysis* / Q000235:genetics; D010641:Phenotype; D012204:Rh-Hr Blood-Group System / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies; C033242:RH-antibodies; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen