Composition of lipid A in the S and R forms of Shigella dysenteriae serovar 1.
A chemical analysis was performed of lipid A, isolated by acid hydrolysis of the lipopolysaccharide from the S and R forms of Shigella dysenteriae serovar 1. Differences in the moiety of both lipids and sugars were compared. The lipid portion consisted of a homologous series of fatty acids ranging from C12:0 to C18:0 (predominant homologues, C12:0, C14:0 and C16:0) and the homologous series of 3-hydroxy acids ranging from C12:0 to C16:0 (predominant homologue, 3-OH-C14:0). The major sugar portion consisted of D-glucosamine. The toxicity of lipid A in mice (LD50) ranged between 300-400 micrograms/mouse, and values from the Limulus amebocyte lysate assay were recorded as titres of 10(-5) to 10(-6) mg/ml.
Sourek
J
J
National Institute of Public Health, Prague, Czechoslovakia.
Stránský
K
K
Trnka
T
T
Julák
J
J
Levin
J
J
Michalec
C
C
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D007928:Lethal Dose 50; D008033:Limulus Test; D008050:Lipid A / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D008813:Mice, Inbred ICR; D012703:Serotyping; D012762:Shigella dysenteriae / Q000737:chemistry* / Q000145:classification
D002241:Carbohydrates; D005227:Fatty Acids; D008050:Lipid A; D005944:Glucosamine