title: GAC1 may encode a regulatory subunit for protein phosphatase type 1 in Saccharomyces cerevisiae.
abstract: Elevated dosage of the GAC1 gene from the yeast Saccharomyces cerevisiae causes hyperaccumulation of glycogen whereas a gene disruption of GAC1 results in reduced glycogen levels. Glycogen synthase is almost entirely in the active, glucose 6-phosphate-independent, form in cells with increased gene dosage of GAC1 whereas the enzyme is mostly in the inactive form in strains lacking GAC1. GAC1 encodes an 88 kDa protein that is similar to the regulatory subunit (RG1) of phosphoprotein phosphatase type 1 (PP-1) from skeletal muscle that targets PP-1 to glycogen particles. Taken together, these results suggest that GAC1 encodes a regulatory subunit of PP-1. As previously shown for glycogen phosphorylase (GPH1), GAC1 RNA accumulates concomitantly with the appearance of glycogen. A strain with a mutation in the regulatory subunit of the cAMP-dependent protein kinase (bcy1) fails to accumulate GPH1 and GAC1 RNA. These results point to coordinate regulation of enzymes involved in glycogen metabolism at the level of RNA accumulation and indicate that at least part of this control is exerted by the RAS-cAMP pathway.
authors.affiliation: Department of Microbiology, North Carolina State University, Raleigh 27695-7615.
authors.forename: J M
authors.initials: JM
authors.lastname: François
authors.forename: S
authors.initials: S
authors.lastname: Thompson-Jaeger
authors.forename: J
authors.initials: J
authors.lastname: Skroch
authors.forename: U
authors.initials: U
authors.lastname: Zellenka
authors.forename: W
authors.initials: W
authors.lastname: Spevak
authors.forename: K
authors.initials: K
authors.lastname: Tatchell
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D006003:Glycogen / Q000378:metabolism; D006006:Glycogen Synthase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D011494:Protein Kinases / Q000378:metabolism; D054645:Protein Phosphatase 1; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; C073060:GAC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D006003:Glycogen; D000242:Cyclic AMP; D006006:Glycogen Synthase; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1