[Use of monoclonal antibodies against human HLA II antigens for the detection of bovine B lymphocytes and macrophages].
The crossreactivity of mouse monoclonal antibodies (MoAbs) (Tab. I) prepared against human HLA-DR and HLA-DP antigens was studied in various bovine cells: lymphocytes from lymph nodes and peripheral blood, adherent (B) and nonadherent (T) lymphocytes, monocytes, granulocytes and platelets. In the immunofluorescence test, MoAbs Bra13, Bra14, Bra20, Bra22, Bra30, Bra70, HL-38 reacted with bovine B lymphocytes and monocytes, but not with other tested cells (Tab. III, IV). These antibodies, except Bra22, were positive with B lymphocytes in the complement dependent cytotoxic test (Tab. II). The similarity of the bovine antigens and HLA-DR antigens determined by used MoAbs was also proved by immunoblotting. Monoclonal antibodies Bra38 and BraFB6 did not react with the bovine cells and separated antigens. The epitope (HLA-DR) recognized by the antibody Bra38 is probably absent in cattle. The presence of HLA-DP analogue determined by the antibody BraFB6 has not been confirmed. The crossreactive MoAbs could be used for the detection of B lymphocytes and macrophages in veterinary immunology.
Dusinský
R
R
Ustav biochémie a genetiky zivocíchov SAV, Ivanka pri Dunaji.
Simon
M
M
Ujházyová
J
J
Poláková
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D008264:Macrophages / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens
Vyuzitie monoklonálnych protilátok proti l'udským HLA II antigénom na detekciu bovinných B lymfocytov a makrofágov.