The stimulatory and inhibitory effects of glycyrrhizin and a glycyrrhetinic acid derivative on phosphorylation of lipocortin I by A-kinase in vitro.
The effects of GL and oGA on the activity (phosphorylation of lipocortin I) by A-kinase were investigated in vitro. It was found that (i) phosphorylation of the 35-36 kDa polypeptide by A-kinase was inhibited selectively when the partially purified kinase fraction from EAT cells was incubated with [gamma-32P]ATP in the presence of 60 microM oGA; (ii) the marked polypeptide was identified as a lipocortin I; and (iii) the activity of the kinase was stimulated about 4-fold by 5 microM oGA, but it was inhibited at doses above 25 microM. The drug-induced inhibition of phosphorylation of lipocortin I by A-kinase may be implicated in the anti-inflammatory effect of the drugs.
Ohtsuki
K
K
Department of Bioscience, Kitasato University School of Hygienic Sciences, Sagamihara, Japan.
Oh-Ishi
M
M
Nagata
N
N
D000818:Animals; D017305:Annexin A1 / Q000378:metabolism*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D002286:Carcinoma, Ehrlich Tumor; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D019695:Glycyrrhizic Acid; D006801:Humans; D008969:Molecular Sequence Data; D010766:Phosphorylation / Q000187:drug effects; D011494:Protein Kinases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D017305:Annexin A1; D000894:Anti-Inflammatory Agents, Non-Steroidal; D019695:Glycyrrhizic Acid; D011494:Protein Kinases; D006034:Glycyrrhetinic Acid