[Inhibitory effect of transferrin on the binding and maintenance of FSH receptors in rat granulosa cells].
It has been recently demonstrated that transferrin (TRF) can inhibit the functional differentiation of rat granulosa cells, but the mechanism remains unknown. In present experiment, the effect of TRF on the binding of 125I-rFSH to receptors and on the maintenance of FSH receptors in granulosa cells from immature, diethylstilbestrol treated rats were studied. The results show that the physiological range of TRF partially blocked the binding of 125I-rFSH to granulosa cells in a dose-dependent manner. TRF also dose-dependently inhibited the maintenance of FSH receptors in accordance with the reduced production of progesterone and estradiol. In view of the present and our previous investigation, it could be concluded that mechanisms of the inhibitory effect of TRF on the functional differentiation of granulosa cells mainly involve blockade of FSH binding to its receptors and inhibition of FSH receptor maintenance by FSH.
Yu
J H
JH
Department of Reproductive Physiology, Sichuan Family Planning Research Institute, WHO Collaboration Centre for Research in Human Reproduction, Chengdu.
Guo
J
J
Guo
J
J
D000818:Animals; D001665:Binding Sites; D002454:Cell Differentiation; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011962:Receptors, FSH / Q000187:drug effects*; D014168:Transferrin / Q000494:pharmacology*
D011962:Receptors, FSH; D014168:Transferrin; D011374:Progesterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone