[Construction and characterization of shuttle plasmid pBHG1 in Bacillus thuringiensis and Escherichia coli].
The recombinant plasmid pBHGA was constructed by ligating plasmid pHTA1030 from B. thuringiensis to plasmid pJH101 from E. coli. The recombinant plasmid pBHGA was digested and deleted by various restriction enzymes, generating 14 derived recombinant plasmids differing in molecular weight. The expressible analysis for the plasmid pBHG1 from among the derived plasmids in E. coli HB101 and B. subtilis 168, the results proved the recombinant plasmid pBHG1 is carring promotive region, original region and segregational stability region of B. thuringiensis plasmid pHTA1030. The recombinant plasmid pBHG1 can be high frequency transformation in to B. thuringiensis HD-1 Cry- B and high-expression in B. thuringiensis. HD-1 Cry- B. The plasmid pBHG1 is segregational stability in B. subtilis 168 at 45 degrees C. These plasmids may be useful tools for study of plasmid replication and expression in B. thuringiensis once a reproducible transformation system is available for this organism.
Guo
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Labratory of Molecular Biology, Chinese Academy of agricultural sciences.
Fan
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Y
D001413:Bacillus thuringiensis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic