Glycosphingolipid expression in spontaneously aborted fetuses and placenta from blood group p women. Evidence for placenta being the primary target for anti-Tja-antibodies.
A 12-week-old fetus and one 17-week-old fetus + placenta were obtained after spontaneous abortions from two women of blood group p. The 17-week-old fetus was dissected into intestine, liver, brain and residual tissue. Nonacid glycosphingolipid fractions were prepared from the tissues. Glycolipid characterization was carried out using thin layer chromatography immunostained with monoclonal antibodies and bacteria and by 1H NMR spectroscopy and mass spectrometry. In the placental fraction substantial amounts of globotetraosylceramide (P-antigen) and globotriaosylceramide (Pk-antigen) were identified. In contrast, the fetuses contained only trace amounts of these structures, as revealed by immunostaining. These results indicate that the primary target for the antibodies of the anti-Tja serum is the placenta tissue, resulting in termination of the pregnancy.
Lindström
K
K
Department of Medical Biochemistry, University of Göteborg, Sweden.
Von Dem Borne
A E
AE
Breimer
M E
ME
Cedergren
B
B
Okubo
Y
Y
Rydberg
L
L
Teneberg
S
S
Samuelsson
B E
BE
D000022:Abortion, Spontaneous / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D005260:Female; D005333:Fetus / Q000737:chemistry*; D005865:Gestational Age; D006028:Glycosphingolipids / Q000032:analysis*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D006028:Glycosphingolipids