Characterization of aliphatic amine 1,4-diazobicyclo (2,2,2) octane-induced stimulation of beet spinach thylakoid electron transport.
Electron transport activity of beet spinach thylakoids was enhanced in the presence of aliphatic amine, DABCO (1,4-diazobicyclo (2,2,2) octane), a hydrophilic proton trapping agent. The extent of stimulation was pH-dependent and similar to the effect of the uncoupler ammonium chloride on electron transport. The stimulation of whole-chain (H2O-->MV) electron transport activity was observed only at high (rate-saturating) light intensity. The light-induced proton uptake coupled to electron transport of thylakoid was also arrested by DABCO, suggesting that DABCO uncouples thylakoid phosphorylation by proton trapping.
Sabat
S C
SC
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Mohanty
P
P
D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D004579:Electron Transport / Q000187:drug effects; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D010879:Piperazines / Q000494:pharmacology*; D010944:Plants / Q000378:metabolism*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D010879:Piperazines; D011837:Radiation-Protective Agents; C007306:triethylenediamine