Measurement of serum IgE antibodies against Japanese cedar pollen (Cryptomeria japonica) in Japanese monkeys (Macaca fuscata) with pollinosis.
IgE antibodies against allergens of Japanese cedar (Cryptomeria japonica, CJ) pollen in the serum of seven Japanese monkeys (Macaca fuscata) with pollinosis were measured by fluorometric indirect enzyme-linked immunosorbent assay (ELISA). All of the monkeys were found to have specific IgE to the crude pollen antigen. The specific IgE levels were well correlated with those determined by the Pharmacia CAP system. IgE antibodies were then assayed with two kinds of purified allergens (Cry j I and Cry j II) by the ELISA. We found that five monkeys had specific IgE to both allergens, although the other two had IgE only to Cry j I or Cry j II; there is different immune responsiveness to the two major allergens in the monkeys.
Sakaguchi
M
M
Department of Microbiology, Institute of Public Health, Tokyo, Japan.
Inouye
S
S
Imaoka
K
K
Miyazawa
H
H
Hashimoto
M
M
Nigi
H
H
Nakamura
S
S
Gotoh
S
S
Minezawa
M
M
Fujimoto
K
K
D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D052179:Antigens, Plant; D002850:Chromatography, Gel; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007073:Immunoglobulin E / Q000097:blood*; D007136:Immunoglobulins / Q000097:blood; D008251:Macaca / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000097:blood*
D000485:Allergens; D052179:Antigens, Plant; C065290:Cry j I protein, Cryptomeria japonica; C065291:Cry j II protein, Cryptomeria japonica; D007136:Immunoglobulins; D010940:Plant Proteins; D007073:Immunoglobulin E