Relationship between the 5'-leader sequence of mRNAs and the translation efficiency in a eukaryotic in vitro system.
To examine the effect of the 5'-leader region of mRNAs in regulating translation efficiency, chimeric mRNAs which have various sequences of the 5'-leader were synthesized by the SP6 in vitro transcription system, and translated in the wheat germ in vitro translation system. Translation efficiency was affected by the length of the 5'-leader and the number of the codon A-U-G at the translation initiation site.
Wakiyama
M
M
Institute for Biomolecular Science, Gakushuin University, Tokyo, Japan.
Hirao
I
I
Kumagai
I
I
Watanabe
K
K
Miura
K
K
D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D002678:Chimera; D006041:Goats; D007768:Lactalbumin / Q000235:genetics; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics
D012333:RNA, Messenger; D012367:RNA, Viral; D007768:Lactalbumin