Fluorescent fibre-optic immunosensing system based on complement lysis of liposome containing carboxyfluorescein.
A novel type of fibre-optic immunosensing system has been developed. Agar gel-immobilized liposomes containing carboxyfluorescein were attached to the tip of an optical fibre. Complement-mediated immunolysis of the liposome was fluorometrically detected through the fibre. By using dinitrophenyl (DNP) hapten-loaded liposomes, concentrations as low as 500-fold diluted anti-DNP antibody and 0.76 CH50 ml-1 of complement were detected.
Tatsu
Y
Y
Government Industrial Research Institute, Osaka, Japan.
Yamamura
S
S
Yoshikawa
S
S
D015374:Biosensing Techniques; D003165:Complement System Proteins / Q000737:chemistry*; D005336:Fiber Optic Technology; D005452:Fluoresceins; D005453:Fluorescence; D008081:Liposomes / Q000737:chemistry
D005452:Fluoresceins; D008081:Liposomes; C024098:6-carboxyfluorescein; D003165:Complement System Proteins