title: Leukotriene B4-induced human melanocyte pigmentation and leukotriene C4-induced human melanocyte growth are inhibited by different isoquinolinesulfonamides.
abstract: Leukotriene C4 (LTC4) is known to be a potent mitogen for cultured human neonatal melanocytes. We now demonstrate that leukotriene B4 (LTB4) can induce pigmentation in cultured human neonatal melanocytes in a dose-dependent fashion. The LTC4-induced mitogenesis is blocked by the cyclic nucleotide-dependent kinase inhibitor N-[2-(methyl-amino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H8). The LTB4-induced pigmentation is blocked by the protein kinase C inhibitor 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H7). We propose that LTB4-induced pigmentation and LTC4-induced mitogenesis are important in vivo signals. Their different effects in our culture system are blocked by different protein kinase inhibitors.
authors.affiliation: Department of Dermatology, University of Colorado School of Medicine, Denver 80262.
authors.forename: J G
authors.initials: JG
authors.lastname: Morelli
authors.forename: S S
authors.initials: SS
authors.lastname: Hake
authors.forename: R C
authors.initials: RC
authors.lastname: Murphy
authors.forename: D A
authors.initials: DA
authors.lastname: Norris
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology*; D007975:Leukotriene B4 / Q000494:pharmacology*; D008544:Melanocytes / Q000187:drug effects*; D010858:Pigmentation / Q000187:drug effects*; D010879:Piperazines / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D013189:SRS-A / Q000494:pharmacology*
chemical_list: D007546:Isoquinolines; D010879:Piperazines; D013189:SRS-A; D007975:Leukotriene B4; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; D011493:Protein Kinase C