title: Identification of a non-microsomal cinnamic acid 4-hydroxylase from potato tuber (S. tuberosum) and its partial purification.
abstract: The cytoplasmic localisation of cinnamic acid 4-hydroxylase (CA4H) has been shown by isolation and subcellular fractionation of the enzyme in Hepes buffer. The enzyme was purified by ammonium sulphate fractionation followed by AcA-34 molecular sieve chromatography. The enzyme existed as a high molecular mass which dissociated to a lower form on dilution on the column. The pH optimum, sulphydryl requirement, molecular and preliminary kinetic characteristics were investigated.
authors.affiliation: Food Technology and Enzyme Engineering Division, Bhabha Atomic Research Centre, Bombay.
authors.forename: A S
authors.initials: AS
authors.lastname: Deshpande
authors.forename: K K
authors.initials: KK
authors.lastname: Surendranathan
authors.forename: P M
authors.initials: PM
authors.lastname: Nair
mesh_terms: D002413:Cations, Divalent; D002458:Cell Fractionation; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000302:isolation & purification / Q000378:metabolism*; D011198:Solanum tuberosum / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology; D050564:Trans-Cinnamate 4-Monooxygenase
chemical_list: D002413:Cations, Divalent; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D050564:Trans-Cinnamate 4-Monooxygenase