Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism.
Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing ablts. This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline VH segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the pre-B cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.
Usuda
S
S
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Takemori
T
T
Matsuoka
M
M
Shirasawa
T
T
Yoshida
K
K
Mori
A
A
Ishizaka
K
K
Sakano
H
H
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D002461:Cell Line, Transformed; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D004270:DNA, Circular / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000276:immunology
D015342:DNA Probes; D004270:DNA, Circular; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D009838:Oligodeoxyribonucleotides; D004247:DNA