DNA conformation of the region preceding the beta-lactamase promoter of pUC19 which is required for efficient transcription.
The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.
Hirota
Y
Y
Section of Molecular Biology, Meiji Institute of Health Science, Odawara, Japan.
Ohyama
T
T
D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D001618:beta-Lactamases / Q000235:genetics*
D004269:DNA, Bacterial; D001618:beta-Lactamases