Unique and independent parameters (UIP) formulation for thermodynamic models of complex protein-ligand systems.
A method for reformulating the thermodynamic (delta G) description of complex equilibria is presented. The purpose of this reformulation is to take a system of N complexes which is completely defined by N delta Gs, and recast it in terms of a new set of N delta Gs. This reformulation is an extension of the concept of interaction energy (J. Wyman, Adv. Protein Chem. 19 (1964) 223-286). The new delta Gs obtained by this reformulation reflect the intrinsic properties of the binding sites and the hierarchical nature of potential interactions between them. A simple set of rules are developed which allow for the description of complex protein-ligand binding schemes and these rules are used to derive schemes for hemoglobin O2 binding. This reformulation represents the foundation for the theoretical description of the coupling of energy in protein-ligand systems as illustrated by the theoretical analysis of allosterism in a dimeric protein presented in the following paper. This reformulation also provides the foundation for the analysis of data pertaining to complex equilibria.
Gutheil
W G
WG
Department of Chemistry, University of Southern California, Los Angeles 90089-0744.
McKenna
C E
CE
D006454:Hemoglobins / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D046911:Macromolecular Substances; D008432:Mathematical Computing; D008956:Models, Chemical; D010100:Oxygen / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
D006454:Hemoglobins; D008024:Ligands; D046911:Macromolecular Substances; D011506:Proteins; D010100:Oxygen