[Mineralogic research of bone union formation after transplantation of autogenic bone marrow and stromal cells into the bone defect from in vitro culture. Experimental studies. III].
In experimental studies on 3 groups of rabbits (1. with autogenic bone marrow transplantation, 2. with stromal cells from in vitro culture transplantation, 3. control group) the mineralogic changes within healing fracture have been observed. These changes were investigated with scanning microscope and chemically using Cameca electronic microprobe and X-ray diffractometer. In each group there were differences in the time of arising of trabecular structures and their mineralization. Bone trabeculae were visible first in a group of rabbits with performed transplantation of stromal cells. They were present in a great number and their structure was homogeneous. They contained prominent quantity of apatites similar to the one found in normal bone. In the final phase of observation, trabeculae of external part of union demonstrated a parallel orientation and a tendency to transformation into a compact bone. After some time trabeculae arose in rabbits after autogenic bone marrow transplantation. They were less numerous and their structure was disordered. They were characterized by a significant chemical heterogeneity. The content of Ca and P was similar to their content in a normal bone. At the very and trabeculae arose in the control group. Their structure however was heterogeneous. In the final phase of observation the content of Ca and P did not reach the level found in a normal bone. Within the whole bone union a spongy bone arose.
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III Katedry Chirurgii AM, Krakowie.
D000818:Animals; D016026:Bone Marrow Transplantation / Q000502:physiology*; D001842:Bone and Bones / Q000648:ultrastructure; D002113:Calcification, Physiologic / Q000502:physiology*; D002478:Cells, Cultured; D017102:Fracture Healing / Q000502:physiology; D050723:Fractures, Bone / Q000503:physiopathology*; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D017154:Stromal Cells / Q000637:transplantation*; D014961:X-Ray Diffraction
Badania mineralogiczne tworzacego sie zrostu kostnego po przeszczepieniu do ubytku kostnego autogennego szpiku kostnego i komórek zrebowych z hodowli in vitro. Badania eksperymentalne. Cz. III.