Decreased uptake of low density lipoprotein by LLC-PK cells cultured at low magnesium concentration.
LLC-PK cells grown in tissue culture on medium containing normal or deficient amounts of magnesium were presented with 125I-low density lipoprotein (LDL) and the uptake determined. Total LDL uptake by Mg-deficient cells decreased as the magnesium concentration decreased. The receptor binding of LDL and the internalization of LDL by the Mg-deficient cells were reduced. Degradation of LDL by Mg-deficient cells, when corrected for the reduced uptake, was less affected.
Mahfouz
M M
MM
Burnsides Research Laboratory, University of Illinois, Urbana.
Smith
T L
TL
Kummerow
F A
FA
D000818:Animals; D002460:Cell Line; D006801:Humans; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008275:Magnesium Deficiency / Q000378:metabolism*; D013499:Surface Properties; D013552:Swine
D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL