[Ischemia and reperfusion: the production and release of malondialdehyde, oxypurines and nucleosides in the isolated rat heart].
Ischemia and reperfusion damage has been evaluated by determining the sum of adenine nucleotides, nucleosides, oxypurines and the concentration of malondialdehyde, ascorbic acid, lactate and pyruvate in the isolated rat heart subjected to global normothermic ischemia and subsequent reperfusion. In addition, the sum of oxypurines and nucleosides, and the concentration of malondialdehyde has been determined in the perfusate collected during the reperfusion. Data indicate that ischemia and reperfusion induce an oxidative stress to myocardial tissue (increase of tissue malondialdehyde and decrease of ascorbic acid, and release of malondialdehyde during reperfusion) that, due to the output of relevant amount of oxypurines (congruent to 6.7 mumol/g dry weight) and of nucleosides (congruent to 7.0 mumol/30/min/g dry weight), it is not even able to restore its energy metabolism after reperfusion.
Lazzarino
G
G
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Centro per lo Studio dell'Ipossia ed Iperossia Tissutale, Università degli Studi Tor Vergata, Roma.
Tavazzi
B
B
Di Pierro
D
D
Giardina
B
B
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism*; D009705:Nucleosides / Q000032:analysis / Q000378:metabolism*; D011688:Purinones / Q000032:analysis / Q000378:metabolism*; D051381:Rats
D009705:Nucleosides; D011688:Purinones; D008315:Malondialdehyde
Ischemia e riperfusione: produzione e rilascio di malondialdeide, ossipurine e nucleosidi nel cuore isolato di ratto.