Production of high efficiency of plating hepatitis A virus in primary African green monkey kidney cells.
High-titered hepatitis A virus, strain HM-175, was produced in primary African green monkey kidney cells (5.5 x 10(10) tissue culture ID50/850 cm2 roller bottle). The virus preparation had an efficiency of plating of 15 particles per infectious unit. Single-cycle growth kinetics of the adapted virus indicated that after an eclipse period of 2 days, maximal yields were attained 6 days after infection.
Nadala
E C
EC
Department of Microbiology, University of Hawaii, Honolulu 96822.
Loh
P C
PC
D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006507:Hepatovirus / Q000254:growth & development*; D007668:Kidney / Q000382:microbiology*