Lambda CM8, a human sequence with putative centromeric function, does not map to the centromere but is present in one to two copies at 9qter.
A DNA fragment isolated from a human genomic library, was reported to be present at all human centromeres and present at 16-32 copies per genome. Reintroduction of this DNA into mammalian cells as a concatenated phage clone gave rise to dicentric chromosomes which gave rise to a new, stable, chromosome. Taken together these observations could mean that this DNA is part of a native centromere. We have reexamined the location and copy number of this sequence and find it to be present at 1-2 copies per genome with a single site of in situ hybridisation at 9qter.
McGill
N I
NI
Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Fantes
J
J
Cooke
H
H
D015139:Blotting, Southern; D002503:Centromere / Q000502:physiology*; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI; D015698:Genomic Library; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D008214:Lymphocytes / Q000166:cytology; D015183:Restriction Mapping
D004247:DNA; D015246:Deoxyribonuclease EcoRI