title: Isoelectric focusing patterns of beta-lactamases in the rapidly growing mycobacteria.
abstract: beta-lactamases from 259 strains of rapidly growing mycobacteria that included the third biovariant complex of Mycobacterium fortuitum, M. peregrinum, M. abscessus, M. chelonae, the M. chelonae-like organisms (MCLO), and M. smegmatis were analyzed by isoelectric focusing (IEF). All isolates produced acidic beta-lactamases with major band isoelectric points (pIs) between 4.4 and 6.0. Each of the 6 taxonomic groups exhibited 1 or 2 characteristic beta-lactamase IEF patterns. Heterogeneity among IEF patterns was evident in 5 of the 6 groups, however, and was greatest among the third biovariant complex of M. fortuitum. beta-lactamase patterns correlated with previously identified taxonomic subgroups of M. smegmatis and the third biovariant complex of M. fortuitum. beta-lactamase IEF analysis of MCLO strains isolated from two outbreaks demonstrated its possible usefulness for epidemiologic evaluation.
authors.affiliation: Department of Microbiology, University of Texas Health Center, Tyler 75710.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: R J
authors.initials: RJ
authors.lastname: Wallace
authors.forename: V A
authors.initials: VA
authors.lastname: Steingrube
authors.forename: B A
authors.initials: BA
authors.lastname: Brown
authors.forename: R
authors.initials: R
authors.lastname: Nash
authors.forename: A
authors.initials: A
authors.lastname: Silcox
authors.forename: M
authors.initials: M
authors.lastname: Tsukamura
mesh_terms: D000818:Animals; D002415:Cats; D006801:Humans; D007525:Isoelectric Focusing; D009161:Mycobacterium / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D016926:Mycobacterium chelonae / Q000201:enzymology; D009170:Nontuberculous Mycobacteria / Q000201:enzymology; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001618:beta-Lactamases