A novel study on the effect of acetylsalicylic acid on the binding capacity of estrogen receptors from MCF-7 cells.
The effect of acetylsalicylic acid (ASA, aspirin) was investigated on [3H]-estradiol-17 beta ([3H]-E2) binding to estrogen receptors (ER) from MCF-7 mammary tumour cells. No effect was observed using the monoclonal estrogen receptor-enzyme immunoassay (ER-EIA). In contrast to the ER-EIA, the radioreceptor assay (RRA) showed decreases of [3H]-E2 specific binding in the presence of increasing amounts of ASA. It is therefore concluded that these results demonstrate that ASA possibly affects the direct binding of E2 to region E of the ER of human breast cancer MCF-7 cells, but does not affect binding of monoclonal antibodies to regions D and F of the ER.
van Aswegen
C
C
Department of Urology, University of Pretoria, HF Verwoerd Hospital, Republic of South Africa.
Dirksen van Schalkwyk
J C
JC
Roux
L J
LJ
Becker
P J
PJ
Du Plessis
D J
DJ
D000911:Antibodies, Monoclonal; D001241:Aspirin / Q000494:pharmacology*; D001665:Binding Sites; D001943:Breast Neoplasms / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D011869:Radioligand Assay; D011960:Receptors, Estrogen / Q000187:drug effects* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D011960:Receptors, Estrogen; D004958:Estradiol; D001241:Aspirin