title: Differential regulation of protein synthesis in smooth muscle cells from normotensive and spontaneously hypertensive rats by a three-dimensional matrix of type I collagen.
abstract: OBJECTIVE
  The objective of this study was to gain insight into the metabolic and morphological properties of smooth muscle cells (SMC) from spontaneously hypertensive rats (SHR) when cultured in vivo under similar conditions.
  
  
  METHODS
  Three-dimensional cell-collagen systems represent living tissue equivalents in vitro and simulate natural conditions more closely than conventional monolayer cultures.
  
  
  METHODS
  The effect of a three-dimensional matrix of type I collagen on ultrastructure, total protein and collagen synthesis and cell cycle distribution of SMC from SHR and normotensive Wistar-Kyoto (WKY) rats was studied.
  
  
  RESULTS
  Collagen lattice-cultured SMC from SHR and WKY rats showed the synthetic phenotype, i.e. the cytoplasm was filled with organelles characteristic of secretory protein synthesis. There was a decrease in the percentage of cells in the 5 phase compared with monolayer cultures. Total protein synthesized by SMC from SHR and WKY rats in lattices was lowered compared with monolayer cultures. However, reduction of protein synthesis in SMC from SHR was less than in SMC from WKY rats. Differences in the proportion of collagen in SMC from SHR and WKY rats were not demonstrable in collagen lattice cultures.
  
  
  CONCLUSIONS
  The present study suggests that a three-dimensional matrix of type I collagen may modulate total protein synthesis in SMC from SHR and WKY rats. However, cells from SHR react less to this matrix than those from WKY rats.
authors.affiliation: Institute for Arteriosclerosis Research, University of Münster, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Thie
authors.forename: B
authors.initials: B
authors.lastname: Schüssler
authors.forename: H
authors.initials: H
authors.lastname: Robenek
authors.forename: W
authors.initials: W
authors.lastname: Zidek
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis* / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D005434:Flow Cytometry; D006973:Hypertension / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
chemical_list: D016326:Extracellular Matrix Proteins; D003094:Collagen