[Production of progesterone in short term tissue culture by follicular cells of women].
After volume and content of the follicular cells were determined the concentration of LH, FSH and prolactin was estimated in the ovarian follicular fluid taken during laparotomy of 17 women (ages 14-43). The sixty six hour follicular fluid cells cultures were established and the concentration of the medium progesterone were assessed by means of radioimmunological methods. The ability of cells to produce progesterone was demonstrated in 3/4 of the cases. It has been found that the progesterone producing cells come from the follicles with significantly higher LH and prolactin concentration. The positive correlation was observed between FSH concentration in follicular fluid and culture progesterone production by the cells in 42 and 66 hour of the experiments.
Putowski
L
L
II Kliniki Ginekologii Operacyjnej Akademii Medycznej, Lublinie.
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis; D006801:Humans; D007986:Luteinizing Hormone / Q000032:analysis; D006080:Ovarian Follicle / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis*; D011388:Prolactin / Q000032:analysis; D012016:Reference Values
D011374:Progesterone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
Wytwarzanie progesteronu w krótkiej hodowli komórek ziarnistych jajnika kobiety.