Phosphorylation of a surface receptor bound urokinase-type plasminogen activator in a human metastatic carcinomatous cell line.
The 32P-labeled urokinase (uPA) bound to surface receptors of Detroit 562 cells was immunoprecipitated by anti-uPA antibody. Amino acid analysis showed that tyrosines and serines were the acceptors. Inhibition of protein kinases greatly reduced the 32P incorporation, suggesting that the respective cellular src gene product and protein kinase C were involved in the phosphorylations. Proteins purified on chromatographic columns contained two forms of uPA, a high (HMW) and a low (LMW) molecular weight. Tyrosine-phosphorylation occurs in the HMW and A-chain. Such modifications might modulate the extracellular activities of uPA.
Takahashi
K
K
Department of Physiology, Shimane Medical University, Izumo, Japan.
Kwaan
H C
HC
Ikeo
K
K
Koh
E
E
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000255:Adenosine Triphosphate / Q000378:metabolism; D015153:Blotting, Western; D002277:Carcinoma; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D019833:Genistein; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D009362:Neoplasm Metastasis; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D010879:Piperazines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D055293:Receptors, Urokinase Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
D007529:Isoflavones; D007546:Isoquinolines; C524898:PLAUR protein, human; D010761:Phosphorus Radioisotopes; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000255:Adenosine Triphosphate; D019833:Genistein; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D014568:Urokinase-Type Plasminogen Activator