Platelet activating factor in culture media as an indicator of human embryonic development after in-vitro fertilization.
Although human chorionic gonadotrophin can detect trophoblast after implantation of the conceptus, there is a need to detect the conceptus before implantation. We have investigated whether human embryo-derived platelet activating factor is formed during embryonic development after in-vitro fertilization. A total of 99 ova from 12 patients were cultured and the 54 media were analysed. Platelet activating factor was also measured by radioimmunoassay after extraction. Fertilization increased the amount of platelet activating factor 4-fold over non-fertilized ova to a level of 4 ng/ml. This increase was also dependent on the degree of embryonic development with a maximum level of platelet activating factor of 7 ng/ml at the 2-cell stage. The follicular inducing agent used to treat the patient also had an effect on platelet activating factor; buserelin treatment gave embryos with a higher level than did clomiphene citrate treatment. These results indicate that platelet activating factor may have a role in embryonic development before implantation and may serve as a useful marker for fertilization and the developmental stage of the embryo.
Nakatsuka
M
M
Department of Obstetrics and Gynaecology, Okayama University Medical School, Japan.
Yoshida
N
N
Kudo
T
T
D000328:Adult; D002064:Buserelin / Q000494:pharmacology / Q000627:therapeutic use; D002970:Cleavage Stage, Ovum; D002996:Clomiphene / Q000627:therapeutic use; D046508:Culture Techniques; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D006080:Ovarian Follicle / Q000033:anatomy & histology; D010062:Ovulation Induction / Q000379:methods; D010972:Platelet Activating Factor / Q000096:biosynthesis* / Q000302:isolation & purification; D011863:Radioimmunoassay
D010972:Platelet Activating Factor; D002996:Clomiphene; D002064:Buserelin