RNA binding characteristics of a 16 kDa glycine-rich protein from maize.
We have previously described a developmentally regulated mRNA in maize that accumulates in mature embryos and is involved in a variety of stress responses in the plant. The sequence of the encoded 16 kDa protein (MA16) predicts that it is an RNA-binding protein, since it possesses a ribonucleoprotein consensus sequence-type RNA-binding domain (CS-RBD). To assess the predicted RNA binding property of the protein and as a starting point to characterize its function we have used ribohomopolymer-binding assays. Here we show that the MA16-encoded protein binds preferentially to uridine- and guanosine-rich RNAs. In light of these results a likely role for this protein in RNA metabolism during late embryogenesis and in the stress response is discussed.
Ludevid
M D
MD
Departamento de Genética Molecular, Centro de Investigación y Desarrollo, Barcelona, Spain.
Freire
M A
MA
Gómez
J
J
Burd
C G
CG
Albericio
F
F
Giralt
E
E
Dreyfuss
G
G
Pagès
M
M
D000595:Amino Acid Sequence; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D011066:Poly C / Q000378:metabolism; D011068:Poly G / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D003313:Zea mays / Q000235:genetics*
D006360:Heat-Shock Proteins; D010940:Plant Proteins; D016601:RNA-Binding Proteins; C073976:GRP protein, Zea mays; D011061:Poly A; D011068:Poly G; D011072:Poly U; D011066:Poly C