title: [Preparation and regeneration of protoplasts of the strain Streptomyces hygroscopicus 155].
abstract: Protoplasts of Streptomyces hygroscopicus 155 producing nigericin, a polyether antibiotic, were prepared. Conditions for protoplasting and optimal concentrations of lysozyme and glycine, and the age of mycelium were studied. With the method of the experiment design, concentrations of four components: CaCl2, MgCl2, KH2PO4 and TES buffer in the regeneration medium were determined. The levels of CaCl2 and TES buffer proved to be the most important parameters.
authors.forename: K R
authors.initials: KR
authors.lastname: Khristova
authors.forename: V T
authors.initials: VT
authors.lastname: Petkova
authors.forename: V N
authors.initials: VN
authors.lastname: Danilenko
authors.forename: I V
authors.initials: IV
authors.lastname: Ivanova
authors.forename: B V
authors.initials: BV
authors.lastname: Bakalov
mesh_terms: D003470:Culture Media; D005998:Glycine / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D009550:Nigericin / Q000096:biosynthesis*; D011523:Protoplasts / Q000378:metabolism*; D012038:Regeneration / Q000502:physiology*; D013302:Streptomyces / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D003470:Culture Media; D009113:Muramidase; D009550:Nigericin; D005998:Glycine
vernacular_title: Poluchenie i regeneratsiia protoplastov shtamma Streptomyces hygroscopicus 155.