title: The influence of dietary fat on the interaction of lymphocytes with high endothelial venules.
abstract: The specific adherence of lymphocytes to high endothelial venules (HEV) represents the first step in the lymphocyte emigration from blood into most lymphoid tissues. The interaction of lymphocytes with HEV exhibits a remarkable organ specificity, which appears to be mediated by complementary receptors on both recirculating lymphocytes (homing receptors) and tissue-specific HEV (vascular addressins). The expression of homing receptors varies and depends on factors such as lymphocyte subtype, stage of activation and maturation. As these receptors are glycoproteins, which are anchored in the cell membrane, it can be envisaged that their position and function are determined by the overall composition of the cell membrane itself. In this study we investigated the significance of dietary fat concentration and saturation for the interaction between lymphocytes and HEV. In addition to these functional studies, the expression of homing receptors in combination with T and B cell markers were analyzed. Using immunohistochemistry the effect on the presence and characteristics of lymphocytes and HEV in situ was studied. Changes in the dietary fatty acid composition resulted in an altered ability of T and B cells to adhere to HEV, without affecting their binding preference. The changed adhesion patterns seemed to be associated with alterations in the expression of adhesion molecules, that are essential for the lymphocyte migration. The latter observation might in turn be explained by the observed modifications in the fatty acid composition of the lymphocytes. These results suggest a role for the fatty acid composition of the nutrition in the process of lymphocyte recirculation.
authors.affiliation: Department of Histology, Free University, Amsterdam, The Netherlands.
authors.forename: A J
authors.initials: AJ
authors.lastname: Twisk
authors.forename: F A
authors.initials: FA
authors.lastname: Rutten
authors.forename: K
authors.initials: K
authors.lastname: Schornagel-Hoeben
authors.forename: G
authors.initials: G
authors.lastname: Kraal
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects*; D005260:Female; D008055:Lipids / Q000097:blood; D016131:Lymphocyte Subsets / Q000187:drug effects*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D010938:Plant Oils / Q000494:pharmacology; D016201:Receptors, Lymphocyte Homing / Q000187:drug effects*; D000074242:Sunflower Oil; D014699:Venules
chemical_list: D015815:Cell Adhesion Molecules; D004041:Dietary Fats; D008055:Lipids; D008562:Membrane Glycoproteins; D010938:Plant Oils; D016201:Receptors, Lymphocyte Homing; D000074242:Sunflower Oil; C029310:lard