Inhibition of in vitro myogenic differentiation by a polyomavirus early function.
In the present work we report on the role of a polyomavirus (Py) early function in interfering with both morphological and biochemical differentiation of the myogenic C2 cell line. The analysis of cell clones stably transfected with a plasmid carrying an ORI- Py genome showed that in the presence of the whole viral early region myogenesis is blocked and a transformed phenotype is evident. By using a plasmid that only encodes large-T function, the involvement of this individual early viral gene product was determined. Inhibition of myogenic differentiation by Py large T is proportional to the level of its expression. This inhibition does not appear to require alteration of cell growth properties. The analysis of muscle-specific functions expressed at different steps in the myogenic pathway showed that Py large T blocks the expression of terminal differentiation markers without altering the expression of the regulatory gene MyoD.
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Dipartimento di Biopatologia Umana, Università di Roma La Sapienza, Italy.
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D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division; D003673:Defective Viruses; D051379:Mice; D009124:Muscle Proteins / Q000096:biosynthesis; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D017570:MyoD Protein; D018008:Myogenin; D009687:Nuclear Proteins / Q000096:biosynthesis*; D010750:Phosphoproteins / Q000096:biosynthesis*; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D000952:Antigens, Polyomavirus Transforming; D009124:Muscle Proteins; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; C503801:Myog protein, mouse; D018008:Myogenin; D009687:Nuclear Proteins; D010750:Phosphoproteins