[Detection of DNA G+C mol% in taxonomy of Streptococcus mutans by means of high performance liquid chromatography].
In the present study, DNA G+C mol% of 9 strains of bacteria (including 7 international standard strain, Ingbritt, and 2 other strains extracted in our Lab) were detected with application of High Performance Liquid Chromatograph. The results showed that DNA G+C content of S. mutans was 35-40%, S. sobrinus was 45-46%, S. cricetus was 38%, and S. rattus was 41%. The difference between S sobrinus and S. mutans was higher than 5%, which suggested that S. sobrinus should be identified as an independent classification unit of bacterial species from S. mutans.
Ling
J Q
JQ
Dental Hospital, Hubei Medical College, Wuhan.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003596:Cytosine / Q000032:analysis*; D004269:DNA, Bacterial / Q000737:chemistry*; D006147:Guanine / Q000032:analysis*; D051381:Rats; D013295:Streptococcus mutans / Q000145:classification* / Q000235:genetics; D016986:Streptococcus sobrinus / Q000145:classification / Q000235:genetics
D004269:DNA, Bacterial; D006147:Guanine; D003596:Cytosine