Significance of western blot in diagnosis of cysticercosis cellulosae.
Sodium dodecyl sulphate (SDS) polyacrylamide gradient gel electrophoresis (PAGE) and immunoblot were used for the diagnosis of patients with cysticercosis cellulosae. 26 KD protein band of the whole Cysticercus antigen showed a high specificity to the sera from patients with cysticercosis cellulosae and had no cross reaction with sera from normal controls and other parasitic diseases including hydatidosis. The positive rate of Western blot with 26 KD band was 70% and the results showed no significant difference in comparison with those of CFT and ELISA.
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Beijing Tropical Medicine Research Institute, WHO Collaborating Center for Paragonimiasis, Clinorchiasis and Leishmaniasis.
Liu
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D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D015153:Blotting, Western; D003551:Cysticercosis / Q000175:diagnosis*; D003552:Cysticercus / Q000276:immunology*; D006801:Humans
D000947:Antigens, Helminth