Analysis of human hepatic microsomal glucose-6-phosphatase in clinical conditions where the T2 pyrophosphate/phosphate transport protein is absent.
The availability of a rare set of human hepatic microsomes in which T2, a pyrophosphate/phosphate transport protein of the glucose-6-phosphatase system, has been shown immunologically to be completely absent, has permitted further characterization of multicomponent glucose-6-phosphatase (EC 3.1.3.9). Pyrophosphatase activity in intact microsomes was found to be totally absent, but was normal in disrupted microsomes. However, Pi did not accumulate within the lumen of the microsomes when glucose 6-phosphate was the substrate. This was not as predicted if there is only one transport protein in the endoplasmic reticulum capable of transporting Pi, produced by glucose-6-phosphatase, out of the lumen. The results suggest that the pyrophosphate/phosphate transport system of human hepatic endoplasmic reticulum must be more complex than previously thought, as it must comprise at least two protein components.
Nordlie
R C
RC
Department of Biochemistry and Molecular Biology, School of Medicine, University of North Dakota, Grand Forks 58202.
Scott
H M
HM
Waddell
I D
ID
Hume
R
R
Burchell
A
A
D000328:Adult; D002352:Carrier Proteins / Q000502:physiology*; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D006801:Humans; D007223:Infant; D007700:Kinetics; D008099:Liver / Q000196:embryology; D008862:Microsomes, Liver / Q000201:enzymology*; D028044:Phosphate-Binding Proteins; D010710:Phosphates / Q000378:metabolism
D002352:Carrier Proteins; D005958:Glucosephosphates; D028044:Phosphate-Binding Proteins; D010710:Phosphates; D019298:Glucose-6-Phosphate; D005952:Glucose-6-Phosphatase