[Study of the mechanistic model for substrate-enzyme interaction on rabbit thymus tissue-based biosensor].
The authors studied the possible mechanistic model for substrate-enzyme interaction on rabbit thymus tissue-based biosensor by tissue chemistry and potentiometric method. The results show that cell construction of coarsely ground and immobilized fresh rabbit thymus is mostly intact. The channels of lipid double-molecule in the cell membranes were expanded. This tissue sensing membrane is better than the natural tissue membrane in terms of electrode response. The membrane has been found to be usable for up to 25 days with a response time of 8 minutes and slope of 51.2mV/decade. The authors suggested a new idea of "frame" construction.
Gao
X
X
Li
Y
Y
Yin
G
G
D000818:Animals; D015374:Biosensing Techniques; D002462:Cell Membrane; D008954:Models, Biological; D011817:Rabbits; D013950:Thymus Gland / Q000166:cytology / Q000201:enzymology*