[The conjugation of preliminarily immunosorbent-immobilized 5B4D6 monoclonal antibodies with a chelating polymer].
A study has been performed to investigate the change in antigen-binding capacity of antibodies as a result of their interaction with chelating agents, and polymers under various conditions. It has been demonstrated that antibody immobilization on the sorbent preceded by the antibody conjugation with chelating polymer allows better maintenance of specific activity of 5B4D6 monoclonal antibodies. Such modification yields a 10-fold increase in the antibody-antigen binding as compared with a standard conjugation technique in a mixture.
Mukhamedova
N M
NM
Slinkin
M A
MA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002614:Chelating Agents / Q000737:chemistry*; D004347:Drug Interactions; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000276:immunology; D007164:Immunosorbents; D004369:Pentetic Acid / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D005904:Glial Fibrillary Acidic Protein; D007146:Immunoglobulin lambda-Chains; D007164:Immunosorbents; D011108:Polymers; D011107:Polylysine; D004369:Pentetic Acid
Kon''iugirovanie predvaritel'no immobilizovannykh na immunosorbente monoklonal'nykh antitel 5B4D6 s khelatiruiushchim polimerom.