Ouabain-sensitive Na-K ATPase response in the rabbit iris-ciliary body after lensectomy-vitrectomy.
We studied ouabain-sensitive Na-K adenosine triphosphatase (ATPase) activities in the iris-ciliary body of rabbit eyes after lensectomy-vitrectomy. Changes in enzyme activities were quantitatively investigated in the plasma membrane of iris-ciliary body at 0 or 7 hours and at days 1, 3, 7, and 14. The specific activity of Na-K ATPase rose to significantly higher levels than the control value at 7 hours following surgery, but returned to the baseline value after 7 days. In addition, we evaluated enzyme activities after lensectomy-vitrectomy during which SF6 or silicone oil was injected. The specific activity of Na-K ATPase following the injection of SF6 or silicone oil was significantly higher than the control value at 7 hours and did not return to the normal value even after 14 days. Consensual reaction, demonstrated by increased Na-K ATPase activity, also was found in the contralateral unoperated eyes of SF6- and silicone oil-injected rabbits. The increased Na-K ATPase activity in the iris-ciliary body after experimental surgery may play an important role in restoring swollen tissues.
Mito
T
T
Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan.
Ishiguro
S
S
Tamai
M
M
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002924:Ciliary Body / Q000201:enzymology* / Q000473:pathology; D005459:Fluorides / Q000008:administration & dosage; D007498:Iris / Q000201:enzymology* / Q000473:pathology; D007908:Lens, Crystalline / Q000601:surgery*; D010042:Ouabain / Q000494:pharmacology; D011817:Rabbits; D012824:Silicic Acid; D012825:Silicon / Q000008:administration & dosage; D012827:Silicone Oils / Q000008:administration & dosage; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism*; D014821:Vitrectomy
D012827:Silicone Oils; D012824:Silicic Acid; C035291:hexafluorosilicic acid; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D005459:Fluorides; D012825:Silicon