Sequence specific block of in vitro DNA synthesis with isopropyl phosphotriesters in template oligodeoxyribonucleotides.
We have synthesized four oligodeoxyribonucleotides each bearing an isopropyl phosphotriester at a defined position. These oligomers were used as templates for in vitro DNA synthesis catalyzed by Escherichia coli DNA polymerase I large fragment. Results showed that the phosphotriester inhibits the DNA chain elongation and the level of the inhibition is dependent on the base 5' to the phosphotriester.
Yashiki
T
T
Gene Research Center, Okayama University, Japan.
Yamana
K
K
Nunota
K
K
Negishi
K
K
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis*; D004261:DNA Replication; D015226:Dinucleoside Phosphates / Q000737:chemistry*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013698:Templates, Genetic
D015226:Dinucleoside Phosphates; D009838:Oligodeoxyribonucleotides; D004247:DNA