[Effect of various plasma preparations and i.v. immunoglobulins on the function of lymphocytes and monocytes in vitro].
Transfusion of whole blood or blood components has an immunosuppressive effect on the recipient and induces changes of several immunological parameters. Especially blood plasma or plasma components were suspected to show an immunosuppressive action. We therefore studied in vitro the effects of autologous and homologous fresh frozen plasma (FFP), fresh plasma (FP) and heparin plasma (HP) as well as the influence of different commercial i.v. immunoglobulins (IVIG) on the mixed lymphocyte reaction (MLR), the mitogen-induced lymphocyte transformation and the inhibition of phagocytosis of red blood cells, measured in the monocyte-monolayer assay (MMA). We demonstrate that autologous and homologous plasma inhibit the PHA and ConA response of lymphocytes (p < 0.025). Minimal differences between homo- and autologous plasma were only observed for FP in PHA-induced lymphocytes. IVIG reduced, in a dose-dependent manner, the MLR and PHA response to basal values. In contrast, plasma and IVIG showed only small effects on the PWM response rates. The addition of FFP, FP, HP or IVIG to cultured monocytes resulted in a significant inhibition of red blood cell phagocytosis, ranging from 76 to 87%. We conclude from our study that, besides unspecific mechanisms, an unknown plasma factor which is enriched in the immunoglobulin fraction of plasma is able to suppress the T-cell immune response.
Sibrowski
W
W
Institut für Transfusionsmedizin Westfälische Wilhelms-Universität, Münster.
Brauer
P
P
D002478:Cells, Cultured; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology; D007116:Immunization, Passive; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology; D010949:Plasma / Q000276:immunology*
Wirkung verschiedener Plasmapräparationen und i.v.-Immunoglobuline auf die Funktion von Lymphozyten und Monozyten in vitro.