Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library.
A new approach for the isolation of chromosome-specific subsets from a human genomic yeast artificial chromosome (YAC) library is described. It is based on the hybridization with an Alu polymerase chain reaction (PCR) probe. We screened a 1.5 genome equivalent YAC library of megabase insert size with Alu PCR products amplified from hybrid cell lines containing human chromosome 21, and identified a subset of 63 clones representative of this chromosome. The majority of clones were assigned to chromosome 21 by the presence of specific STSs and in situ hybridization. Twenty-nine of 36 STSs that we tested were detected in the subset, and a contig spanning 20 centimorgans in the genetic map and containing 8 STSs in 4 YACs was identified. The proposed approach can greatly speed efforts to construct physical maps of the human genome.
Chumakov
I M
IM
Centre d'Etude du Polymorphisme Humain, Paris, France.
Le Gall
I
I
Billault
A
A
Ougen
P
P
Soularue
P
P
Guillou
S
S
Rigault
P
P
Bui
H
H
De Tand
M F
MF
Barillot
E
E
D001483:Base Sequence; D015825:Chromosomes, Fungal; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015723:Gene Library; D005821:Genetic Techniques; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D004247:DNA