title: Chromosome anomalies in mouse zygotes treated by growth hormone-releasing factor.
abstract: The incidence of chromosome anomalies was studied in fertilized oocytes in two groups of hybrid mice in which superovulation was induced by gonadotrophins and growth hormone-releasing factor (GRF) supplementation or gonadotrophins alone (controls). The rate of fertilization was significantly higher among GRF-treated females than among controls (74.1 versus 84.7%; P < 0.013). Cytogenetic data were obtained in 262 fertilized oocytes (89 from control females and 173 from GRF-treated females). The frequency of aneuploidy, calculated as twice the frequency of hyperhaploidy was 2.31% in GRF-treated females and 2.24% in controls (NS). The use of GRF to treat female mice did not adversely affect the maturation process of oocytes nor did it induce an increased frequency of aneuploidy.
authors.affiliation: Departament d'Obstetricia i Ginecologia, Institut Universitari Dexeus, Barcelona, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Arán
authors.forename: G
authors.initials: G
authors.lastname: Calderón
authors.forename: M
authors.initials: M
authors.lastname: Moragas
authors.forename: M J
authors.initials: MJ
authors.lastname: Torelló
authors.forename: A
authors.initials: A
authors.lastname: Veiga
authors.forename: P N
authors.initials: PN
authors.lastname: Barri
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D005260:Female; D006064:Gonadotropins, Equine / Q000627:therapeutic use; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology* / Q000627:therapeutic use; D008431:Maternal-Fetal Exchange; D051379:Mice; D010062:Ovulation Induction / Q000379:methods; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D013480:Superovulation; D015053:Zygote / Q000187:drug effects*
chemical_list: D006064:Gonadotropins, Equine; D013007:Growth Hormone-Releasing Hormone