[The insulin-secreting and proliferative activity of established islet cells in the presence of sulfonylurea].
Exponentially growing rat islet cells (RINr) and hamster islet cells (HIT T-15) were incubated in presence of tolbutamide (10-1000 microM), gliclazide (0.1-10 microM) or glibenclamide (0.01-10 microM) for 15 hrs. Accumulation of insulin in culture medium was estimated by RIA. Effects of sulfonylureas (SU) on cell proliferation were assessed by 3H-thymidine (3H-T) incorporation into cellular DNA. All of SUs used stimulated insulin production in RIN and HIT cell cultures (with an exception of tolbutamide, which markedly suppressed insulin secretion in HIT cells at 1000 microM). 3H-T incorporation into RIN cells was elevated only in presence of gliclazide (10 microM), whereas tolbutamide at 1000 M significantly inhibited RIN cell proliferation. Gliclazide (0.1 microM) and glibenclamide (0.01-10 microM) enhanced 3H-T incorporation into HIT cells. Further detailed investigations of mechanisms of SU effects on islet cell reproduction will be of use for designing optimal strategy of hypoglycemizing therapy of diabetes mellitus.
Balabolkin
M I
MI
Timofeev
A V
AV
Danilova
T I
TI
Zhuleva
L Iu
LIu
Ol'shanskaia
A A
AA
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006224:Cricetinae; D005907:Gliclazide / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D007328:Insulin / Q000032:analysis / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D013453:Sulfonylurea Compounds / Q000494:pharmacology*; D013997:Time Factors; D014044:Tolbutamide / Q000494:pharmacology
D007328:Insulin; D013453:Sulfonylurea Compounds; D014044:Tolbutamide; D005907:Gliclazide; D005905:Glyburide
Insulinsekretiruiushchaia i proliferativnaia aktivnost' perevivaemykh ostrovkovykh kletok v prisutstvii sul'fonilmocheviny.