title: Characterization of protein kinase C in Xenopus oocytes.
abstract: Protein kinase C (PKC) was partially purified from Xenopus laevis oocytes by ammonium sulfate fractionation followed by DEAE-cellulose and hydroxyapatite column chromatography. In the latter chromatography, two distinct PKC activities were identified. Both PKC fractions contained an 80 kDa protein which was recognized by three antisera raised against the conserved regions of mammalian PKC. However, specific antisera against alpha, beta I, beta II, and gamma-subspecies of rat PKC did not recognize the protein. Kinetic properties of the Xenopus PKCs were very similar to those of the rat alpha PKC, and only a subtle difference was found in the mode of activation by arachidonic acid. When oocytes were treated with the tumor promoter, phorbol 12-myristate 13-acetate, one of the Xenopus PKCs was found to disappear very rapidly, while the other remained unchanged up to 2 hr.
authors.affiliation: Laboratory of Molecular Biology, Kobe University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sahara
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Aoto
authors.forename: T
authors.initials: T
authors.lastname: Ohnishi
authors.forename: H
authors.initials: H
authors.lastname: Kaise
authors.forename: H
authors.initials: H
authors.lastname: Koide
authors.forename: K
authors.initials: K
authors.lastname: Ogita
authors.forename: Y
authors.initials: Y
authors.lastname: Fukami
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D002273:Carcinogens / Q000494:pharmacology; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D004075:Diglycerides / Q000494:pharmacology; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005260:Female; D006882:Hydroxyapatites; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D046911:Macromolecular Substances; D008970:Molecular Weight; D009865:Oocytes / Q000201:enzymology*; D010743:Phospholipids / Q000494:pharmacology; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D014982:Xenopus laevis
chemical_list: D002273:Carcinogens; D004075:Diglycerides; D006882:Hydroxyapatites; D007527:Isoenzymes; D046911:Macromolecular Substances; D010743:Phospholipids; D017886:Durapatite; D011493:Protein Kinase C