Ethanol production from cellulose by coupled saccharification/fermentation using Saccharomyces cerevisiae and cellulase complex from Sclerotium rolfsii UV-8 mutant.
Using cellulase/hemicellulase complex of Sclerotium rolfsii UV-8 mutant and Saccharomyces cerevisiae for fermentation, the coupled saccharification/fermentation (CSF) of 15% AT-rice straw was carried out at 40 degrees C, pH 4.5 for the first 24 h and further incubation was performed at 30 degrees C for 72 h. Increasing the amount of cellulase activity from 3-12 IU FPA/g of substrate resulted in increased yields of ethanol from 1.5-3.6% in 96 h. It has been observed that the coupled system was advantageous over the two stage (separate hydrolysis/fermentation) system as it produced higher amounts of ethanol from cellulose (3.6% as compared to 2.3% ethanol from rice straw).
Deshpande
M V
MV
Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005658:Fungi / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D016296:Mutagenesis; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D014466:Ultraviolet Rays; D001617:beta-Glucosidase / Q000032:analysis
D000431:Ethanol; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase