Measurements of tumor blood flow using intraperitoneal deuterium and 2H-NMR spectroscopy.
Using i.p. delivered deuterated water, and 2H NMR spectroscopy, it is possible to non-invasively, serially, and quantitatively measure blood flow through tissue volumes as small as 0.05 ml. Measurements made with this technique show that changes in total tumor blood flow reflect local changes measured superficially by laser Doppler flowmetry, and that tumors of identical histology and size have heterogeneous blood flow rates. Finally, we have shown that tumors growing in an irradiated normal tissue have a lower blood flow rate which is manifest when tumors grow beyond 0.1 ml.
Okunieff
P
P
Dept. of Radiation Medicine, Massachusetts General Hospital, Boston 02114.
Lee
J
J
Itoh
M
M
Vaupel
P
P
D000818:Animals; D001783:Blood Flow Velocity; D003903:Deuterium / Q000008:administration & dosage; D005069:Evaluation Studies as Topic; D005354:Fibrosarcoma / Q000098:blood supply*; D007274:Injections, Intraperitoneal; D017078:Laser-Doppler Flowmetry / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008809:Mice, Inbred C3H
D003903:Deuterium