RNase protection assays and RNA gel blots: a direct comparison of sensitivity.
RNase protection assays are commonly thought to be a more sensitive means of detecting and quantitating specific mRNAs than are RNA gel blots (Northern blots). We have directly compared the sensitivity of these two approaches by assaying for known amounts of in vitro synthesized beta-glucuronidase mRNA. With the probes and protocols employed here, the ability to detect a specific mRNA was similar whether RNase protection or RNA gel blot analyses were performed.
Higgs
D C
DC
Department of Botany, Iowa State University, Ames 50011.
Colbert
J T
JT
D002523:Edible Grain; D004591:Electrophoresis, Polyacrylamide Gel; D005966:Glucuronidase / Q000235:genetics; D015336:Molecular Probe Techniques; D009691:Nucleic Acid Denaturation; D015347:RNA Probes; D012329:RNA, Bacterial / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D012260:Ribonucleases / Q000378:metabolism; D012680:Sensitivity and Specificity
D015347:RNA Probes; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012260:Ribonucleases; D005966:Glucuronidase