Photosensitized formation of 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) in DNA by riboflavin.
Potosensitized formation of 8-hydroxyguanine in DNA by riboflavin was observed. A reaction mechanism involving guanine radical cation and hydration reaction was proposed. This hypothesis was confirmed by the incorporation of [18O]-atom within guanine moiety in isotopic experiments using [18O]-H2O. Photosensitized formation of oh8Gua by riboflavin was also observed in cellular DNA.
Kasai
H
H
Biology Division, National Cancer Center Research Institute, Tokyo, Japan.
Yamaizumi
Z
Z
Yamamoto
F
F
Bessho
T
T
Nishimura
S
S
Berger
M
M
Cadet
J
J
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry* / Q000187:drug effects / Q000528:radiation effects; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008027:Light; D051379:Mice; D017319:Photosensitizing Agents / Q000494:pharmacology*; D012256:Riboflavin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D017319:Photosensitizing Agents; C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA; D012256:Riboflavin