Selection of a human chromosome 21 enriched YAC sub-library using a chromosome-specific composite probe.
The subdivision of total genomic human yeast artificial chromosome (YAC) libraries into specific chromosome clone collections will greatly facilitate the construction of an integrated genetic, physical and transcriptional map of the genome. We report the isolation of 388 YAC clones from a human library with an average insert size of 620 kilobases (kb) by the hybridization of a composite chromosome 21 probe to a high-density array of YAC clones. Roughly half of these clones hybridize to chromosome 21 by fluorescence in situ hybridization. These clones represent a twofold coverage of the chromosome. The technique offers the potential of sub-dividing whole genomic YAC libraries into their chromosomal elements to produce high-resolution tools for genome mapping.
Ross
M T
MT
Imperial Cancer Research Fund, Lincoln's Inn Fields, London, UK.
Nizetíc
D
D
Nguyen
C
C
Knights
C
C
Vatcheva
R
R
Burden
N
N
Douglas
C
C
Zehetner
G
G
Ward
D C
DC
Baldini
A
A
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015723:Gene Library; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics
D005819:Genetic Markers; D009838:Oligodeoxyribonucleotides; D004247:DNA