title: Effects of base damages on DNA replication--mechanism of preferential purine nucleotide insertion opposite abasic site in template DNA.
abstract: DNA polymerase preferentially inserts purine nucleotides opposite non-instructive lesions such as abasic sites during DNA replication. In order to elucidate the mechanism of the preferential insertion, a DNA template containing a model abasic site and primers containing 4 different nucleotides (A,G,C,T) at primer terminus were synthesized. The stability of the primer terminus nucleotide placed opposite the abasic site was evaluated on the basis of its sensitivity to 3'-5' exonuclease associated with DNA polymerase.
authors.affiliation: Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ide
authors.forename: H
authors.initials: H
authors.lastname: Murayama
authors.forename: A
authors.initials: A
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Morii
authors.forename: K
authors.initials: K
authors.lastname: Makino
mesh_terms: D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004249:DNA Damage; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D011684:Purine Nucleosides / Q000235:genetics*; D013698:Templates, Genetic
chemical_list: D011684:Purine Nucleosides; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V