title: Platelet-derived growth factor is angiogenic in vivo.
abstract: PDGF receptors have recently been found to be expressed in microvascular endothelium in vivo under circumstances of endothelial cell activation and angiogenesis suggesting that PDGF may have a direct effect on endothelial cells. We have tested the angiogenic activity of PDGF-AA and -BB homodimers in the chick chorioallantoic membrane in vivo. PDGF-BB was found to consistently induce an angiogenic response whereas PDGF-AA was less active. Morphological analyses revealed that there was little inflammation associated with this response but an increase in vessel density suggested a direct effect of PDGF on embryonic chorioallantoic endothelial cells. In vitro, PDGF-BB was found to be more potent than PDGF-AA in stimulating the chemotaxis of rat brain capillary endothelial cells. This is consistent with a direct effect of PDGF on endothelial cells. Thus, this novel angiogenic activity of PDGF has implications for several developmental and pathological events in which PDGF, particularly the B-chain, is expressed.
authors.affiliation: Max-Planck-Institut für Psychiatrie, Abteilung Neurochemie, Martinsried, FRG.
authors.forename: W
authors.initials: W
authors.lastname: Risau
authors.forename: H
authors.initials: H
authors.lastname: Drexler
authors.forename: V
authors.initials: V
authors.lastname: Mironov
authors.forename: A
authors.initials: A
authors.lastname: Smits
authors.forename: A
authors.initials: A
authors.lastname: Siegbahn
authors.forename: K
authors.initials: K
authors.lastname: Funa
authors.forename: C H
authors.initials: CH
authors.lastname: Heldin
mesh_terms: D000482:Allantois / Q000098:blood supply; D000818:Animals; D001921:Brain / Q000098:blood supply; D002196:Capillaries / Q000166:cytology; D002633:Chemotaxis; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology; D046911:Macromolecular Substances; D009389:Neovascularization, Pathologic; D010982:Platelet-Derived Growth Factor / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
chemical_list: D046911:Macromolecular Substances; D010982:Platelet-Derived Growth Factor