Properties of hypotonized, crosslinked and crosslinked- permeabilized rat erythrocytes as potential carrier systems.
The osmotic fragility curves of isotonic rat RBCs, studied at pH 8 to avoid the Hb insolubility, are similar to those in humans at pH 7.4. Hypotonized rat RBCs, either directly or dialysed (0-24 h), are more hemolysis-resistant than isotonic rat RBCs. The discocyte-stomatocyte-spherocyte transformation can be observed with scanning electron microscopy. Protein crosslinking with dimethyl suberimidate can stabilize RBCs. The crosslinking level (60%), the cellular yield (80%), the mechanical and hemolytic resistance and the protective effect of enzyme activities, were studied in crosslinked or crosslinked- permeabilized RBCs after digitonin treatment. The normal discocytic shape of RBCs under scanning electron microscopy becomes stomatocytic in crosslinked and crosslinked- permeabilized RBCs with an erosioned surface.
Luque
J
J
Departamento de Bioquímica y Biologia Molecular, Universidad de Alcalá, Madrid, Spain.
Garín
M I
MI
Sanz
S
S
Ropero
P
P
Pinilla
M
M
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003432:Cross-Linking Reagents; D004120:Dimethyl Suberimidate; D004337:Drug Carriers; D004906:Erythrocyte Count / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D006461:Hemolysis / Q000502:physiology; D006801:Humans; D007038:Hypotonic Solutions; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical
D003432:Cross-Linking Reagents; D004337:Drug Carriers; D007038:Hypotonic Solutions; D004120:Dimethyl Suberimidate