Effect of natural beta-interferon on estrogen receptor mRNA of breast cancer cells.
We have demonstrated that natural beta-interferon (beta-IFN) enhances estrogen receptor (ER) mRNA of a human breast cancer cell line, CG-5. Cells were sensitive to the effect of beta-IFN at concentrations ranging from 10 to 100 IU/ml. The increase of ER mRNA was seen after 48 hr of treatment in at least three separate experiments. Our results are in agreement with the previously observed enhancement of receptor protein. In addition, they suggest that the IFN-induced promotion of the antiproliferative activity of drugs which act via ER may be due, in part, to increased receptor synthesis.
Sica
G
G
Istituto di Istologia ed Embriologia, Università Cattolica del Sacro Cuore, Roma, Italy.
Angelucci
C
C
Iacopino
F
F
Lama
G
G
Robustelli della Cuna
G
G
Serlupi-Crescenzi
O
O
Donini
S
S
D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology*; D007700:Kinetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D015347:RNA Probes; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D016899:Interferon-beta