Endothelin and structurally related analogs distinguish between endothelin receptor subtypes.
The endothelin (ET) analog ET-1[1,3,11,15-Ala] was compared with ET-1, ET-2, ET-3 and sarafotoxins (SRTX) S6b and S6c for receptor binding and function. All the peptides exhibited high affinity binding and contracted rabbit pulmonary artery with near equal potency. In rat aorta both ET-3 and ET-1 [1,3,11,15-Ala] bound with much lower affinity than ET-1 while ET-3 displayed weak contractile potency and ET-1 [1,3,11,15-Ala] and SRTX-c were inactive. In rat left atria, ET-1 [1,3,11,15-Ala] and SRTX-c were weak inhibitors of binding and were also functionally inactive, whereas ET-1, ET-2, ET-3, and SRTX-b were equipotent in producing contractile responses. The data support the idea of there being a predominance of ETA receptors in rat aorta and ETB receptors in rabbit pulmonary artery. In rat left atria, the ET receptor could not be readily classified into ETA or ETB and suggests the existence of a new receptor subtype.
Panek
R L
RL
Department of Pharmacology, Warner-Lambert Company, Ann Arbor, Michigan 48105.
Major
T C
TC
Hingorani
G P
GP
Doherty
A M
AM
Taylor
D G
DG
Rapundalo
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ST
D000818:Animals; D001667:Binding, Competitive; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000378:metabolism* / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011956:Receptors, Cell Surface / Q000145:classification* / Q000187:drug effects; D017466:Receptors, Endothelin; D014757:Viper Venoms / Q000378:metabolism* / Q000494:pharmacology
D016232:Endothelins; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D014757:Viper Venoms; C057354:sarafotoxins s6; C061779:endothelin 1, Ala(1,3,11,15)-