title: Glycerol shock treatment facilitates purification of herpes simplex virus.
abstract: We describe an improved method for purification of herpes simplex virus. The primary improvement is the use of glycerol shock treatment to lyse infected cells. This is a rapid and simple technique that efficiently extracts the cytoplasm from the nucleus, while preserving nuclear integrity. Nearly 100% cell lysis is achieved by this method, and cytoplasmic virus yield is optimal. Other improvements to increase virus yield and purity and to economize on equipment are described.
authors.affiliation: Department of Veterinary Medicine and Surgery, University of Texas M.D. Anderson Cancer Center, Houston 77030.
authors.forename: M
authors.initials: M
authors.lastname: Sarmiento
authors.forename: W W
authors.initials: WW
authors.lastname: Batterson
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000382:microbiology; D005990:Glycerol / Q000494:pharmacology*; D007038:Hypotonic Solutions; D018139:Simplexvirus / Q000302:isolation & purification*; D014709:Vero Cells / Q000187:drug effects; D014764:Viral Proteins / Q000032:analysis
chemical_list: D007038:Hypotonic Solutions; D014764:Viral Proteins; D005990:Glycerol