Activation of human neutrophils by mastoparan. Reorganization of the cytoskeleton, formation of phosphatidylinositol 3,4,5-trisphosphate, secretion up-regulation of complement receptor type 3 and superoxide anion production are stimulated by mastoparan.
In human neutrophils, mastoparan induced rapid F-actin polymerization which was followed by a slow and sustained depolymerization to below the initial F-actin content. Incubation of neutrophils with pertussis toxin inhibited mastoparan-stimulated actin polymerization; however it did not prevent sustained depolymerization of F-actin. Analyses of phospholipids performed in parallel revealed that mastoparan stimulated rapid formation of phosphatidylinositol 3,4,5-trisphosphate (PIP3) and consumption of phosphatidylinositol 4,5-bisphosphate (PIP2). Pertussis toxin treatment blocked mastoparan-induced formation of PIP3. Furthermore, mastoparan stimulated the release of N-acetylglucosaminidase from primary granules. Cytochalasin B enhanced mastoparan-stimulated secretion. Mastoparan triggered superoxide radical production in a cytochalasin B-sensitive manner and induced complement type 3 receptor (CR3) up-regulation.
Norgauer
J
J
Rudolf-Bucheim-Institut für Pharmakologie, Justus-Liebig-Universität, Giessen, Federal Republic of Germany.
Eberle
M
M
Lemke
H D
HD
Aktories
K
K
D000118:Acetylglucosaminidase / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D005434:Flow Cytometry; D005609:Free Radicals; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010455:Peptides; D037342:Pertussis Toxin; D010743:Phospholipids / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D015854:Up-Regulation; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D014862:Wasp Venoms / Q000494:pharmacology*
D000199:Actins; D005609:Free Radicals; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D010743:Phospholipids; D010566:Virulence Factors, Bordetella; D014862:Wasp Venoms; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; C037500:mastoparan; D037342:Pertussis Toxin; D000118:Acetylglucosaminidase