title: [Study of antigenic structure of HIV-1 protein p24 using monoclonal antibodies].
abstract: During the experiments 4 murine and 3 rat hybridomas producing monoclonal antibodies (MAb) against the protein p24 of human immunodeficiency virus type 1 (HIV-1) have been obtained. Using the immunoblotting technique, it was established that all the species of MAb reacted with the same viral proteins which are derivatives of gag gene--p24 and p55. The properties of MAb have been studied in competitive binding. Their ability of binding to different fragments of the gag protein produced by the recombinant plasmids in E. coli cells have been investigated in ELISA. The analysis of the findings suggests that the HIV-1 protein p24 contains at least 3 antigenic epitopes. All species of MAb reacted with 3 different HIV-1 strains and 2 HIV-1 isolates, but failed with 2 different HIV-2 strains. The only MAb NS5E4 can be used as an immunosorbent in the antigenic capture reaction.
authors.forename: E E
authors.initials: EE
authors.lastname: Konovalov
authors.forename: V B
authors.initials: VB
authors.lastname: Loktev
authors.forename: A G
authors.initials: AG
authors.lastname: Pokrovskiĭ
authors.forename: N V
authors.initials: NV
authors.lastname: Fediuk
authors.forename: A I
authors.initials: AI
authors.lastname: Chernykh
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Nechaev
authors.forename: S A
authors.initials: SA
authors.lastname: Kuliandin
authors.forename: S M
authors.initials: SM
authors.lastname: Kipriianov
authors.forename: V I
authors.initials: VI
authors.lastname: Ofitserov
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000382:microbiology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D001667:Binding, Competitive / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D016655:HIV Core Protein p24 / Q000032:analysis* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D016655:HIV Core Protein p24
vernacular_title: Izuchenie antigennoĭ struktury belka p24 VICh-1 s pomoshch'iu monoklonal'nykh antitel.