Description of Leishmania equatorensis sp. n (Kinetoplastida: Trypanosomatidae), a new parasite infecting arboreal mammals in Ecuador.
Characterization is given of a new parasite, Leishmania equatorensis sp. n., which was isolated from the viscera of a sloth (Choloepus hoffmanni) and a squirrel (Sciurus granatensis), captured in humid tropical forest on the Pacific Coast of Ecuador. Data based on biological and molecular criteria, as well as numerical zymotaxonomical analysis, indicate that this parasite is a new species of the L. braziliensis complex. L. equatorensis is clearly distinguishable from all other known species within this complex, using the following molecular criteria: reactivity patterns with specific monoclonal antibodies, isoenzyme electrophoresis, and restriction-endonuclease fragment patterns of kinetoplast DNA (k-DNA).
Grimaldi Júnior
G
G
Departamento de Imunologia, Instituto Oswaldo Cruz, RJ, Brasil.
Kreutzer
R D
RD
Hashiguchi
Y
Y
Gomez
E A
EA
Mimory
T
T
Tesh
R B
RB
D000818:Animals; D000911:Antibodies, Monoclonal; D016054:DNA, Protozoan / Q000032:analysis; D004484:Ecuador; D004591:Electrophoresis, Polyacrylamide Gel; D007891:Leishmania / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification; D012589:Sciuridae / Q000469:parasitology*; D012896:Sloths / Q000469:parasitology*
D000911:Antibodies, Monoclonal; D016054:DNA, Protozoan