title: Detection of hepatitis C virus (HCV) RNA in paraffin embedded tissue sections of human liver of non-A, non-B hepatitis patients by in situ hybridization.
abstract: Localization of hepatitis C virus (HCV) RNA was investigated by non-radioactive in situ hybridization in human liver specimens of chronic non-A, non-B (NANB) hepatitis patients who were seropositive for antibodies to HCV (anti-HCV). For in situ hybridization, T-T dimerized synthetic oligodeoxynucleotide probes were used and DNAs hybridized in situ were detected immunohistochemically using specific antibodies against T-T dimer. The data demonstrates that HCV-RNA was localized in the cytoplasm of hepatocytes in human liver biopsies obtained from the patients with chronic NANB hepatitis seropositive for anti-HCV.
authors.affiliation: 2nd Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Koji
authors.forename: M
authors.initials: M
authors.lastname: Nozawa
authors.forename: K
authors.initials: K
authors.lastname: Kiyosawa
authors.forename: P K
authors.initials: PK
authors.lastname: Nakane
mesh_terms: D000293:Adolescent; D001483:Base Sequence; D015342:DNA Probes; D005260:Female; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000382:microbiology*; D006521:Hepatitis, Chronic / Q000382:microbiology; D006801:Humans; D008099:Liver / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D015342:DNA Probes; D012367:RNA, Viral