Cytotoxicity of human beta-interferon produced in human glioma cells transfected with its gene by means of liposomes.
The cytotoxicity of human beta-interferon (HuIFN-beta) produced in human glioma cells was examined by use of our liposomes entrapping two plasmids, pSV2neo and pSVMTV-IFN-beta. After the cells had been transfected with these genes by means of the liposomes, neomycin-resistant cells were selected. When the selected cells were subjected to a single exposure to dexamethasone, all of the cells were found to produce HuIFN-beta and were eliminated by 8 days. Accordingly, the effect of HuIFN-beta produced in human glioma cells is considered to be cytocidal.
Yoshida
J
J
Department of Neurosurgery, Nagoya University School of Medicine, Japan.
Mizuno
M
M
Yagi
K
K
D016923:Cell Death; D003907:Dexamethasone / Q000494:pharmacology; D005910:Glioma; D006801:Humans; D016899:Interferon-beta / Q000235:genetics / Q000633:toxicity*; D008081:Liposomes; D014162:Transfection; D014407:Tumor Cells, Cultured
D008081:Liposomes; D016899:Interferon-beta; D003907:Dexamethasone