[An experimental study model of human neo-angiogenesis. Heterotopic graft of the cornea in nude mice].
Two grafting methods were devised to transplant heterotopically human corneas to nude mice. Half of them were secured on the mesenterium, enclosed in a small intestine ring; the others were stitched to the anterior abdominal muscles after removal of a muscular disc. We used theses procedures to implant 13 human corneas: 7 according to the first technique and 6 tho the second one. Most of the corneas observed from Day 3 to Day 90 remained transparent along the whole grafting period. The grafts were surgically removed from Day 3 to Day 90 and submitted to an histological examination. It was not observed any morphological changes, versus normal human cornea and especially none neovascularization. These observations are fully in agreement with the persistent transparency of the corneas. With this model, many experimental studies in vivo on human corneas can be planed and conducted without any ethical restriction.
Angioi-Duprez
K
K
Service d'Ophthalmologie Hôpital de Brabois, Vandoeuvre-les-Nancy.
George
J L
JL
D000818:Animals; D003315:Cornea / Q000098:blood supply; D016039:Corneal Transplantation; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic; D013997:Time Factors; D016042:Transplantation, Heterotopic
Un modèle d'étude expérimentale de la néoangiogenèse humaine. La greffe hétérotopique de cornée chez la souris nude.