A PCR method for introducing mutations into cloned DNA by joining an internal primer to a tagged flanking primer.
Stappert
J
J
Max-Planck-Institut für Immunbiologie, Freiburg, FRG.
Wirsching
J
J
Kemler
R
R
D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D016296:Mutagenesis / Q000235:genetics*; D009838:Oligodeoxyribonucleotides / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D009838:Oligodeoxyribonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes