title: A temperature-sensitive CHO-K1 cell mutant (tsTM13) defective in chromosome decondensation and spindle deconstruction in M phase.
abstract: A temperature-sensitive CHO-K1 cell mutant, tsTM13, exhibited a delayed cell cycle progression from metaphase to telophase at a nonpermissive temperature and was finally arrested from anaphase to telophase. Metaphase chromosomes were overcondensed and chromosome disjunction in anaphase was uncoordinated. In telophase, sister chromatids were segregated and cytokinesis was completed, but chromosome structure remained in a condensed state and the spindle was not deconstructed. The level of phosphorylation of histones H1 and H3 remained high in the later stages of mitosis and the activity of histone H1 kinase was also maintained at a high level. These results strongly suggest that the pleiotropic defects of tsTM13 cells in mitosis are associated with a lack of inactivation of activated histone H1 kinase.
authors.affiliation: Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tsuji
authors.forename: Y
authors.initials: Y
authors.lastname: Matsudo
authors.forename: K
authors.initials: K
authors.lastname: Ajiro
authors.forename: H
authors.initials: H
authors.lastname: Yasuda
authors.forename: F
authors.initials: F
authors.lastname: Hanaoka
authors.forename: A
authors.initials: A
authors.lastname: Hayashi
authors.forename: S
authors.initials: S
authors.lastname: Utsumi
authors.forename: Y
authors.initials: Y
authors.lastname: Ohba
authors.forename: T
authors.initials: T
authors.lastname: Hori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D002875:Chromosomes / Q000378:metabolism*; D006224:Cricetinae; D003703:Demecolcine / Q000494:pharmacology; D005434:Flow Cytometry; D005816:Genetic Complementation Test; D006657:Histones / Q000378:metabolism; D007700:Kinetics; D008938:Mitosis / Q000187:drug effects / Q000235:genetics*; D008940:Mitotic Index; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010766:Phosphorylation; D006656:Protamine Kinase / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism*; D013696:Temperature
chemical_list: D006657:Histones; D006656:Protamine Kinase; D003703:Demecolcine