Bone marrow stromal cells attenuate mitoxantrone cytotoxicity against HL-60 leukemic cells.
We evaluated the effect of cultured human bone marrow (BM) stromal cells on the cytotoxicity of mitoxantrone (MIT) against the HL-60 leukemic cell line. BM-derived fibroblastoid cells (BMFC) suppressed colony formation of HL-60 cells in the absence of MIT. BMFC increased the survival of HL-60 colony-forming cells in the presence of 50 ng/ml MIT. A significant decrease in the drug concentration was not detected on BMFC. Our results suggest that BM stromal cells can modulate the antileukemic effect of an anticancer drug.
Takahashi
A
A
Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Yamamoto
K
K
Okuma
M
M
Sasada
M
M
D002448:Cell Adhesion; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D008942:Mitoxantrone / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology; D014410:Tumor Stem Cell Assay
D010835:Phytohemagglutinins; D008942:Mitoxantrone