An SV40 transformation revertant due to a host mutation: isolation and complementation analysis.
We have isolated an SV40 transformation revertant cell line, CL1L, by selection for normal cells whose growth is inhibited under low serum conditions. This line expresses a single, wild-type copy of large T antigen, yet is not transformed. It is not retransformable by transfection of SV40 DNA or infection with a recombinant retrovirus encoding large T antigen. Resistance to transformation therefore appears to be due to a cellular mutation. Fusion of CL1L cells to normal 3T3 cells or SV40-transformed cells results in somatic cell hybrids that are transformed, indicating that resistance is recessive. In addition, fusion of CL1L cells to another SV40 transformation-resistant line, A27, results in transformed hybrids, indicating the existence of discrete complementation groups with respect to SV40 transformation.
Silverstein
G H
GH
Graduate Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor 48109-0620.
Kohrman
D C
DC
Christensen
J B
JB
Brockman
W W
WW
Imperiale
M J
MJ
D016475:3T3 Cells; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism; D002455:Cell Division; D002459:Cell Fusion; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000235:genetics; D005808:Genes, Recessive; D005816:Genetic Complementation Test; D006822:Hybrid Cells / Q000166:cytology; D051379:Mice; D009857:Oncogenes; D011485:Protein Binding; D013539:Simian virus 40 / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral