title: Induction of cyclin mRNA and cyclin-associated histone H1 kinase during liver regeneration.
abstract: Cyclins and cyclin-associated cdc kinases are key regulators of oocyte maturation (Maller, J. L. (1990) in The Biology and Medicine of Signal Transduction (Nishizuka, Y., Endo, M., and Tanaka, C., eds) pp. 323-328, Raven Press, New York), yeast cell cycles (Nurse, P. (1990) Nature 344, 503-508), DNA replication in cell-free systems (D'Urso, F., Marraccino, R. L., Marshak, R. R., and Roberts, J. M. (1990) Science 250, 786-791), and amphibian cell proliferative transitions (Hunt, T. (1991) Nature 350, 462-463). The extent to which these regulatory molecules participate in the growth control of differentiated epithelial cells like hepatocytes is unknown. Therefore, we investigated the expression of "G1" (E, C, and D) and "G2/M" (A, B1, and B2) cyclin mRNAs, the relative levels of cyclin A- and B1-associated histone H1-kinase activity, and the appearance of cyclin-associated kinases (p32/p33cdk2 and p33/p34cdc2) in regenerating rat liver and in control tissues from sham hepatectomized rats. To do this, we exploited a battery of human cyclin cDNAs and cyclin antisera that recognize rat molecules. The results suggest an apparent sequence of regeneration-specific changes: 1) elevated and induced expression of cyclins E (2.1 kilobases (kb)) and C (4 kb), and D mRNAs (4 kb), within 12 h, respectively; 2) induction of cyclins A (3.4 and 1.8 kb), B1 (2.5 and 1.8 kb), and B2 (1.9 kb) mRNAs at 24 h; 3) induction of cyclin A- and B1-associated nuclear histone H1 kinase at 24 h; and 4) enhanced levels of PSTAIRE-containing proteins of Mr approximately 32-33 and 33-34 kDa in nuclear extracts from 24-h regenerating liver that co-immunoprecipitate with cyclin A and B1 antisera, respectively. These observations provide an intellectual framework that unifies the biology of hepatocyte mitogenesis, proto-oncogene expression, and the machinery of the cell cycle.
authors.affiliation: Department of Pharmacology, School of Medicine, University of California, San Diego 92093-0636.
authors.forename: X P
authors.initials: XP
authors.lastname: Lu
authors.forename: K S
authors.initials: KS
authors.lastname: Koch
authors.forename: D J
authors.initials: DJ
authors.lastname: Lew
authors.forename: V
authors.initials: V
authors.lastname: Dulic
authors.forename: J
authors.initials: J
authors.lastname: Pines
authors.forename: S I
authors.initials: SI
authors.lastname: Reed
authors.forename: T
authors.initials: T
authors.lastname: Hunter
authors.forename: H L
authors.initials: HL
authors.lastname: Leffert
mesh_terms: D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002453:Cell Cycle; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000502:physiology; D008115:Liver Regeneration; D006656:Protamine Kinase / Q000096:biosynthesis*; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats
chemical_list: D016213:Cyclins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D006656:Protamine Kinase; D016203:CDC2 Protein Kinase