Interaction between oleic acid-containing pH-sensitive and plain liposomes. Fluorescent spectroscopy studies.
The energy transfer method has been applied to study the interaction between pH-sensitive liposomes (phosphatidyl ethanolamine/oleic acid/cholesterol, 4:2:4 molar ratio) and plain liposomes (phosphatidyl choline/phosphatidyl ethanolamine/cholesterol, 4:2:3 molar ratio). It was shown that a slow fusion process occurs between two types of liposomes. Also, the transfer of oleic acid from pH-sensitive liposomes to plain liposomes takes place. This transfer results in the increased permeability of both pH-sensitive and plain liposomes, facilitating the release of liposome-entrapped fluorescent dye. The data obtained were used for a possible explanation of the mechanism of intracytoplasmic drug delivery by pH-sensitive oleic acid-containing liposomes.
Torchilin
V P
VP
Center for Imaging and Pharmaceutical Research, Massachusetts General Hospital-East, Charlestown 02129.
Lukyanov
A N
AN
Klibanov
A L
AL
Omelyanenko
V G
VG
D004337:Drug Carriers / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry*; D008561:Membrane Fusion; D019301:Oleic Acid; D009829:Oleic Acids / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D004337:Drug Carriers; D008081:Liposomes; D009829:Oleic Acids; D019301:Oleic Acid