title: Fluorescent-labeled oligonucleotides that exhibit a measurable signal in the presence of complementary DNA.
abstract: Oligonucleotide derivatives with a fluorescent dye were designed for exhibiting a measurable signal only when they bind to complementary DNA in aqueous solution. The oligonucleotide with a dansyl group at the specific 2'-sugar residue was synthesized by using the protected 2'-dansylaminouridine phosphorobisamidite. The dansyl-oligonucleotide conjugate binds to its complementary DNA to form duplex with a normal stability and exhibits enhanced fluorescence together with a blue-shift in emission maxima after the hybridization. Another possible candidate involved the use of pyrene-excimer emission upon forming ternary complex between two pyrene-labeled oligonucleotide probes with target DNA. A new and general method for introduction of a pyrene fluorophore into the 3'- or 5'-terminal hydroxyl group of oligonucleotides via different linkers was developed.
authors.affiliation: Department of Applied Chemistry, Himeji Institute of Technology, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamana
authors.forename: Y
authors.initials: Y
authors.lastname: Ohashi
authors.forename: K
authors.initials: K
authors.lastname: Nunota
authors.forename: M
authors.initials: M
authors.lastname: Aoki
authors.forename: H
authors.initials: H
authors.lastname: Nakano
authors.forename: O
authors.initials: O
authors.lastname: Sangen
mesh_terms: D004247:DNA / Q000032:analysis*; D003619:Dansyl Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000737:chemistry
chemical_list: D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D004247:DNA