title: Source of prolactin in human follicular fluid.
abstract: To analyze whether prolactin (PRL) in human follicular fluid (FF) is synthesized locally or derived from the circulation, PRL concentrations of plasma and FF were determined in the patients after ovarian stimulations. The amounts of PRL messenger ribonucleic acid (mRNA) in the follicular tissues during different menstrual phases were also determined. The FF PRL concentration was correlated positively with plasma PRL and highest estradiol levels during the stimulatory cycle. No PRL mRNA sequence was detected in the RNAs extracted from follicles at any stage in the menstrual cycle, although beta-actin mRNA was detected in all samples. In a comparison with pituitary RNA, the PRL mRNA concentration in ovarian follicular tissues seemed to be 10,000 times less than that in the pituitary. These results suggest that FF PRL may not be synthesized locally, but derived from the pituitary via the circulation through passive diffusion, and thus regulated by estrogen.
authors.affiliation: Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohwaki
authors.forename: N
authors.initials: N
authors.lastname: Suganuma
authors.forename: H
authors.initials: H
authors.lastname: Seo
authors.forename: A
authors.initials: A
authors.lastname: Nawa
authors.forename: F
authors.initials: F
authors.lastname: Kikkawa
authors.forename: O
authors.initials: O
authors.lastname: Narita
authors.forename: N
authors.initials: N
authors.lastname: Matsui
authors.forename: Y
authors.initials: Y
authors.lastname: Tomoda
mesh_terms: D000199:Actins / Q000096:biosynthesis; D015152:Blotting, Northern; D004958:Estradiol / Q000096:biosynthesis / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry*; D005498:Follicular Phase / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008183:Luteal Phase / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D011247:Pregnancy / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis / Q000097:blood; D011388:Prolactin / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D000199:Actins; D012333:RNA, Messenger; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin