Chain length distribution of the products formed in solanesyl diphosphate synthase reaction.
Factors that affect the termination of isoprenoid chain elongation catalyzed by prenyltransferase were investigated. The chain-length distribution of reaction products of solanesyl diphosphate synthase [EC 2.5.1.11] homogeneously purified from Micrococcus luteus changed dramatically according to the concentration of the complex formed between isopentenyl diphosphate and Mg2+ (IPP-Mg) in the reaction mixture. However, the concentration of the complex between farnesyl diphosphate and Mg2+ (FPP-Mg), the priming substrate for this synthase, did not affect the product distribution, provided that the concentration of IPP-Mg was maintained at a certain level. Thus, the level of IPP-Mg is decisive in affecting the chain length distribution of the products of the prenyltransferase reaction, and the Mg(2+)-dependent variability of product specificity so far observed can now be understood in terms of the effect of IPP-Mg concentration.
Ohnuma
S
S
Institute for Chemical Reaction Science, Tohoku University, Miyagi.
Koyama
T
T
Ogura
K
K
D019883:Alkyl and Aryl Transferases; D001665:Binding Sites; D002122:Calcium Chloride / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D015636:Magnesium Chloride / Q000494:pharmacology; D016982:Micrococcus luteus / Q000201:enzymology*; D011106:Polyisoprenyl Phosphates / Q000302:isolation & purification / Q000378:metabolism*; D012717:Sesquiterpenes; D014166:Transferases / Q000302:isolation & purification / Q000378:metabolism*
D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; D015636:Magnesium Chloride; C004808:farnesyl pyrophosphate; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C022644:trans-octaprenyltranstransferase; D002122:Calcium Chloride