Tubulin dimer formation via the release of alpha- and beta-tubulin monomers from multimolecular complexes.
The functional subunit of microtubules is a heterodimer consisting of alpha- and beta-tubulin. An understanding of tubulin dimerization has been hampered because it has not proved possible to purify native tubulin monomers. To study the process whereby tubulin dimers are formed, we made use of tubulins synthesized by in vitro transcription and translation. We present evidence that the in vitro synthesis of different mouse alpha-tubulin isotypes involves a multimolecular complex. The synthesis of mouse beta-tubulin isotypes also involves the formation of multimolecular complexes, though different isotypes behave somewhat differently from one another. The properties of in vitro synthesized alpha- and beta-tubulin multimolecular complexes strongly suggest that they are intermediates in the biosynthesis of tubulin monomers. Upon release, these monomers can exchange with pre-existing tubulin heterodimers.
Zabala
J C
JC
Departamento de Biologia Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain.
Cowan
N J
NJ
D002474:Cell-Free System; D046911:Macromolecular Substances; D014404:Tubulin / Q000138:chemical synthesis / Q000737:chemistry*
D046911:Macromolecular Substances; D014404:Tubulin