Acid DNase activities in peripheral, mononuclear blood cells: a possible parameter to detect proliferating cell populations.
After electrophoresis in DNA-containing polyacrylamide gels, two acid DNase activities can be detected in peripheral, mononuclear cells of the human blood. One of these acid DNase activities correlates with cell proliferation; its isoelectrical point is at pI 7.4. By means of this DNase activity, a quantity of less than 1% leukemic cells can be detected. The increased acid DNase activity can indicate the proliferation of malignant cell populations and possibly the proliferation of cell populations during immunological reactions.
Reitz
M
M
Abteilung für Physiologische Chemie, Universität Mainz, Bundesrepublik Deutschland.
Gutjahr
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D004247:DNA / Q000378:metabolism; D003851:Deoxyribonucleases / Q000097:blood* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000201:enzymology* / Q000276:immunology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000201:enzymology*; D012016:Reference Values
D007527:Isoenzymes; D004247:DNA; D003851:Deoxyribonucleases