Expression of the Tn antigen on erythroid cells from a patient with Tn syndrome.
In order to examine expression of the Tn antigen on erythroid cells from a patient with Tn syndrome, we applied a selective two phase liquid culture system for human erythroid progenitors in peripheral blood. The cells were analyzed with flow cytometry employing an anti-Tn antibody and a lectin of Vicia villosa which recognizes only the Tn determinant. In the second phase, the Tn antigen was expressed on the cultured cells from the patient on day 3 and Tn-positive cells reached 62.7% on day 9. On the other hand, Tn-positive cells were not detected in the volunteer's cultured cells. When the patient's cells were co-cultured with the cells from a healthy volunteer, the percentage of Tn-positive cells was much lower than the expected value, suggesting that the normal cells suppressed the expression of Tn antigen on the patient's cells.
Kamitani
Y
Y
Department of Legal Medicine and Human Genetics, Jichi Medical School, Tochigi, Japan.
Kajii
E
E
Suda
T
T
Ikemoto
S
S
D000116:Acetylgalactosamine / Q000276:immunology; D000754:Anemia, Refractory, with Excess of Blasts / Q000097:blood / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D002454:Cell Differentiation; D004903:Erythrocyte Aggregation; D004913:Erythrocytes, Abnormal / Q000201:enzymology / Q000276:immunology; D015672:Erythroid Precursor Cells / Q000201:enzymology / Q000276:immunology*; D005434:Flow Cytometry; D005700:Galactosyltransferases / Q000172:deficiency*; D006386:Hemagglutination Tests; D006402:Hematologic Diseases / Q000097:blood / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology; D008875:Middle Aged; D013577:Syndrome
D000954:Antigens, Surface; D015295:Antigens, Tumor-Associated, Carbohydrate; D008562:Membrane Glycoproteins; C040286:Tn antigen; D005700:Galactosyltransferases; D000116:Acetylgalactosamine