NMR studies on solution structure of single-stranded oligonucleotides causing line broadening.
Unusual line broadening of 1H-NMR lines attributable to the proton (8H) of guanine residues was observed for all tetradeoxyoligonucleotides tested here which have a specific base sequence of dGXXG (X = A or T). For the same samples, line broadening was also obtained in the 31P-NMR spectra. These broadened signals did not become sharp up to 60 degrees C. This unusual spectral phenomenon has been attributed by 2D-NMR and differential NOE to the compact solution structure of the oligonucleotides.
Murakami
A
A
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Suzuki
T
T
Tamura
Y
Y
Nishi
M
M
Morii
T
T
Ide
H
H
Makino
K
K
D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D012996:Solutions; D014867:Water / Q000737:chemistry
D009841:Oligonucleotides; D012996:Solutions; D014867:Water