title: Photosensitized formation of 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) in DNA by riboflavin.
abstract: Potosensitized formation of 8-hydroxyguanine in DNA by riboflavin was observed. A reaction mechanism involving guanine radical cation and hydration reaction was proposed. This hypothesis was confirmed by the incorporation of [18O]-atom within guanine moiety in isotopic experiments using [18O]-H2O. Photosensitized formation of oh8Gua by riboflavin was also observed in cellular DNA.
authors.affiliation: Biology Division, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kasai
authors.forename: Z
authors.initials: Z
authors.lastname: Yamaizumi
authors.forename: F
authors.initials: F
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Bessho
authors.forename: S
authors.initials: S
authors.lastname: Nishimura
authors.forename: M
authors.initials: M
authors.lastname: Berger
authors.forename: J
authors.initials: J
authors.lastname: Cadet
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry* / Q000187:drug effects / Q000528:radiation effects; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008027:Light; D051379:Mice; D017319:Photosensitizing Agents / Q000494:pharmacology*; D012256:Riboflavin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D017319:Photosensitizing Agents; C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA; D012256:Riboflavin