Expression of feline immunodeficiency virus gag gene in Escherichia coli.
The gag gene of a Japanese feline immunodeficiency virus (FIV) isolate, designated as FIV TM 2, was expressed in Escherichia coli as a fusion protein with TrpE. Using this expressed protein, an enzyme-linked immunosorbent assay was developed for detection of antibodies to FIV gag protein in feline sera. With serum samples from a cat experimentally infected with FIV, it was demonstrated that the period of seroconversion detected by this method corresponded to that by Western blotting.
Furuya
T
T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Hasegawa
A
A
Saitoh
M
M
Miyazawa
T
T
Tohya
Y
Y
Hayami
M
M
Takahashi
E
E
Miki
K
K
Mikami
T
T
D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D015683:Gene Products, gag / Q000235:genetics*; D016089:Immunodeficiency Virus, Feline / Q000235:genetics*; D010957:Plasmids; D011993:Recombinant Fusion Proteins
D015683:Gene Products, gag; D011993:Recombinant Fusion Proteins