Kinetics and thermodynamics of double-helix formation of self-complementary deoxyribooctanucleotides d(TCTATAGA) and d(TAGATCTA).
Double-helix formation of self-complementary deoxyribooctanucleotides, d(TCTATAGA) and d(TAGATCTA), with identical nearest neighbor base pairs has been studied by means of UV melting and temperature-jump techniques. The self-complementary duplexes of both octanucleotides with identical nearest neighbors had similar stabilities: The stabilization energies of the octanucleotides at 25 degrees C were 5.8 kcal mol-1 for d(TCTATAGA) and 6.7 kcal mol-1 for d(TAGATCTA). On the kinetic curve the melting reactions finished within 20 ms for d(TCTATAGA) and 40 ms for d(TAGATCTA) at 20 degrees C. For both octanucleotides the rate constants of dissociation increased and the rate constants of association decreased with increasing temperature.
Sugimoto
N
N
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Honda
K
K
Sasaki
M
M
D003854:Deoxyribonucleotides / Q000737:chemistry*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D013816:Thermodynamics
D003854:Deoxyribonucleotides