Characterization of a bovine synovial fluid lubricating factor. III. The interaction with hyaluronic acid.
Although hyaluronate is not a boundary lubricant in cartilaginous and latex:glass bearings, a distinct interaction with purified synovial lubricating factor (PSLF) was demonstrated by three means: 1) enhancement of lubricating ability in an artificial test system; 2) viscometry; 3) electron microscopy. The interaction was of a physical rather than a specific chemical type; it varied with the degree of purification of PSLF and of hyaluronate. The interaction accounts for retention of the relatively small PSLF molecule (approximately 280 kDa) with the synovial mucin on a 0.22 microns filter. The data provide evidence that hyaluronate and PSLF act synergistically in the boundary lubricating activity of animal joints.
Jay
G D
GD
Department of Pathology, State University of New York, Stony Brook.
Lane
B P
BP
Sokoloff
L
L
D000818:Animals; D002356:Cartilage / Q000502:physiology; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D006820:Hyaluronic Acid / Q000737:chemistry / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D008854:Microscopy, Electron; D013582:Synovial Fluid / Q000737:chemistry*; D014783:Viscosity
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C081496:PSLF protein, Bos taurus; D006820:Hyaluronic Acid