Differential response of human interferon-beta promoter elements to trans-activation by HSV VP16 and IRF-1.
The trans-activation potential of herpes simplex virus (HSV) VP16, either alone or in combination with interferon regulatory factor 1 (IRF-1), was examined using hybrid promoters containing different regulatory elements from the interferon-beta promoter. Coexpression of HSV VP16 and IRF-1 differentially activated the AAGTGA hexamer construct Th(2), the AAAGGA hexamer Thm(2) construct, and the natural IFN-beta promoter. Surprisingly, high concentrations of IRF-1 inhibited expression of the PRDII containing reporter P2(1)/CAT. These results indicate that trans-activation by HSV VP16, acting through distinct cellular transcription factors, may be involved in stimulation of IFN-beta regulatory domains.
Leblanc
J F
JF
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec, Canada.
Hiscott
J
J
D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D050856:Interferon Regulatory Factor-1; D016899:Interferon-beta / Q000235:genetics*; D010750:Phosphoproteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D018139:Simplexvirus / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*; D015533:Transcriptional Activation; D014764:Viral Proteins / Q000378:metabolism*
D004268:DNA-Binding Proteins; C494257:IRF1 protein, human; D050856:Interferon Regulatory Factor-1; D010750:Phosphoproteins; D015534:Trans-Activators; D014764:Viral Proteins; D016899:Interferon-beta