[Preparation and utilization of BrdU antiserum. II. Detections of BrdU labelling chromosome and DNA using BrdU antibody technique].
Utilizing highly specific BrdU antiserum, via the second antibody conjugated with enzyme and DAB-H2O2 or AEC-H2O2 colorific methods, the BrdU marked regions of CHO and human chromosomes were detected clearly. The BrdU-DNA prints (pg) on nitrocellulose membranes were also showed with similar methods.
Shang
K
K
Department of Biology, Peking University, Beijing.
Lou
Z
Z
Wu
H
H
D000818:Animals; D001973:Bromodeoxyuridine / Q000276:immunology* / Q000378:metabolism; D016466:CHO Cells; D002875:Chromosomes / Q000737:chemistry*; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera / Q000276:immunology*
D007106:Immune Sera; D004247:DNA; D001973:Bromodeoxyuridine