title: Immunodetection of tubulin carboxypeptidase activity on nitrocellulose membrane after gel electrophoresis and blotting.
abstract: It was found that the detyrosination of tyrosinated tubulin by tubulin carboxypeptidase can occur when both the enzyme and the substrate are adsorbed on nitrocellulose. This, and the use of a specific antibody that recognizes detyrosinated tubulin allowed us to localize tubulin carboxypeptidase on a nitrocellulose membrane after agarose gel electrophoresis and blotting. The method was also extended to detect pancreatic carboxypeptidase A.
authors.affiliation: Centro de Investigaciones en Química Biológica de Córdoba, Facultad de Ciencias Quìmicas, Universidad Nacional de Córdoba, Argentina.
authors.forename: A M
authors.initials: AM
authors.lastname: Smania
authors.forename: C E
authors.initials: CE
authors.lastname: Argaraña
authors.forename: J C
authors.initials: JC
authors.lastname: Weizetfel
authors.forename: H S
authors.initials: HS
authors.lastname: Barra
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D002268:Carboxypeptidases / Q000032:analysis*; D043422:Carboxypeptidases A; D002417:Cattle; D003101:Collodion / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D015151:Immunoblotting; D007525:Isoelectric Focusing; D008567:Membranes, Artificial
chemical_list: D008567:Membranes, Artificial; D003101:Collodion; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; C023212:tyrosyltubulin carboxypeptidase