Immunoperoxidase labelling of previously stained tissue sections: application in oral histopathology.
We have investigated by the avidin-biotin peroxidase technique a wide variety of recognized cellular antigens in paraffin sections of both normal and pathological material which have been previously stained with hematoxylin and eosin. The intensity of peroxidase reaction was adequate to permit the diagnosis and the background of the immunoreaction minimal. This technique therefore, appears useful either for retrospective analysis of cellular antigens or when limited unstained material is available.
Lombardi
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Division of Stomatology and Oral Surgery, School of Dental Medicine, Faculty of Medicine, Geneva, Switzerland.
D000085:Acetates; D019342:Acetic Acid; D004801:Eosine Yellowish-(YS); D005069:Evaluation Studies as Topic; D005404:Fixatives; D005557:Formaldehyde; D006416:Hematoxylin; D006801:Humans; D007124:Immunoenzyme Techniques; D009055:Mouth / Q000378:metabolism / Q000473:pathology*; D010851:Picrates; D013194:Staining and Labeling / Q000379:methods
D000085:Acetates; D005404:Fixatives; D010851:Picrates; D005557:Formaldehyde; C026239:Bouin's solution; D019342:Acetic Acid; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin