title: Analysis of ecdysteroids in the trematodes, Schistosoma mansoni and Fasciola hepatica.
abstract: Adult Schistosoma mansoni from experimentally infected mice and Fasciola hepatica recovered from ovine livers post mortem were analyzed for free and conjugated ecdysteroids by radioimmunoassay, high-performance liquid chromatography monitoring fractions by radioimmunoassay, and by capillary gas chromatography/mass spectrometry (selected ion monitoring). Both species contained ecdysone and 20-hydroxyecdysone as free ecdysteroids and as polar conjugates. F. hepatica also contained a polar conjugate of 2-deoxyecdysone. Evidence of apolar ecdysteroid conjugates was only obtained for F. hepatica. The free ecdysteroid-containing fraction of S. mansoni contained small amounts of unidentified immunoreactive material.
authors.affiliation: Department of Biochemistry, University of Liverpool, U.K.
authors.forename: J M
authors.initials: JM
authors.lastname: Foster
authors.forename: J G
authors.initials: JG
authors.lastname: Mercer
authors.forename: H H
authors.initials: HH
authors.lastname: Rees
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004440:Ecdysone / Q000032:analysis; D026461:Ecdysteroids; D004441:Ecdysterone / Q000032:analysis; D005210:Fasciola hepatica / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007447:Invertebrate Hormones / Q000032:analysis*; D011863:Radioimmunoassay; D012550:Schistosoma mansoni / Q000737:chemistry*; D013256:Steroids / Q000032:analysis*
chemical_list: D026461:Ecdysteroids; D007447:Invertebrate Hormones; D013256:Steroids; D004440:Ecdysone; D004441:Ecdysterone