Antimetastatic activity of boro-amino acid analog protease inhibitors against B16BL6 melanoma in vivo.
Di- and tripeptide boro-amino acid analog protease inhibitors with specificity for chymotrypsin and elastase decrease the number of melanotic foci formed in the lungs of mice in the B16BL6 experimental metastatic tumor model. These effects were at significantly lower concentration than leupeptin or other natural chymotrypsin inhibitors previously reported. These results support the involvement of elastase and chymotrypsin in the metastatic process.
Kinder
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Graduate Program in Pharmacology and Toxicology, College of Pharmacy, Washington State University, Pullman 99164-6510.
Elstad
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Meadows
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Ames
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D000818:Animals; D001897:Boronic Acids / Q000138:chemical synthesis / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000556:secondary; D051379:Mice; D008815:Mice, Inbred Strains; D009362:Neoplasm Metastasis / Q000517:prevention & control; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D015842:Serine Proteinase Inhibitors / Q000138:chemical synthesis / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001897:Boronic Acids; D015842:Serine Proteinase Inhibitors; D002918:Chymotrypsin; D010196:Pancreatic Elastase