Efficacy of various water-soluble chelator molecules in the liposome-mediated macrophage "suicide" technique.
In earlier studies, we described a technique for the "in vivo" depletion of macrophages, using liposome-encapsulated dichloromethylene bisphosphonate (C12MBP). The aim of the present study was to compare the effects on macrophages of various other liposome-delivered Ca2+ chelators: EDTA, DTPA, and EGTA. It appeared that both EDTA and DTPA as well as Ca(2+)-and Cu(2+)-EDTA complexes were also effective in the elimination of splenic macrophages when encapsulated in liposomes and intravenously injected. EGTA, a well-known and frequently used Ca2+ chelator, had no effect.
van Rooijen
N
N
Department of Cell Biology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
Poppema
A
A
D000818:Animals; D002614:Chelating Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004002:Clodronic Acid / Q000008:administration & dosage / Q000494:pharmacology; D004337:Drug Carriers; D004492:Edetic Acid / Q000008:administration & dosage / Q000494:pharmacology; D004533:Egtazic Acid / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D007275:Injections, Intravenous; D008081:Liposomes; D008099:Liver / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D004369:Pentetic Acid / Q000008:administration & dosage / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects
D002614:Chelating Agents; D004337:Drug Carriers; D008081:Liposomes; D004002:Clodronic Acid; D004533:Egtazic Acid; D004369:Pentetic Acid; D004492:Edetic Acid