Regulation of interleukin-1 receptors on AtT-20 mouse pituitary tumour cells.
To study the cellular mechanisms of interleukin-1 (IL-1) in the pituitary corticotroph, we studied the properties of IL-1 receptors on a mouse pituitary ACTH-producing cell line, AtT-20. Scatchard plot analysis revealed a single type of receptor with a Kd (dissociation constant) of 93 pM, and 482 binding sites/cell. [125I]IL-1 alpha binding competed with IL-1 alpha and IL-1 beta in an equimolar fashion. A 24 h pre-incubation with either CRH, epinephrine or nor-epinephrine increased the [125I]IL-1 alpha binding sites in the AtT-20 cells and conversely, a similar pre-incubation with either dexamethasone or tumour necrosis factor-alpha (TNF alpha) decreased them without affecting the affinity of the receptors in either case.
Kobayashi
H
H
Department of Internal Medicine, Kyoto University Faculty of Medicine, Japan.
Fukata
J
J
Tominaga
T
T
Murakami
N
N
Fukushima
M
M
Ebisui
O
O
Segawa
H
H
Nakai
Y
Y
Imura
H
H
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010911:Pituitary Neoplasms / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism*; D017472:Receptors, Interleukin-1; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D007375:Interleukin-1; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D000324:Adrenocorticotropic Hormone