[Effect of anoxia on the growth of cultured neurons from embryonic chick forebrain].
Pure neurons from embryonic day 8 chick forebrain were cultured under anoxic condition (95% N2 and 5% CO2). After 24, 48 and 72 h in culture, MTT colorimetric microassay showed a reduced production of formazan, indicating that the neurons were seriously damaged. In addition, the glucose of the cultured media was significantly depleted. Even when glucose concentration was increased to 800-1,200 mg/100 ml, anoxia still caused neurons to die. The results indicate that brain neurons in embryo are sensitive to anoxia, and any protective influence of glia on anoxic neurons could not be mediated by supplying the latter with glycogen.
Yan
Y Q
YQ
Shanghai Institute of Physiology, Academia Sinica.
Zhou
Z H
ZH
Ji
P
P
Gu
J F
JF
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology*; D002455:Cell Division / Q000502:physiology; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D046508:Culture Techniques; D005947:Glucose; D009474:Neurons / Q000502:physiology
D003470:Culture Media; D005947:Glucose