title: Activation of human complement system Paracoccidioides brasiliensis and its deposition on the yeast form cell surface.
abstract: The yeast form of Paracoccidioides brasiliensis strain Pb18 was able to activate C3 of normal human serum diluted in phosphate-buffered saline or EGTA-MgCl2 in vitro. C3 convertase was also permissive when Pb18 cells were pre-treated with a pool of immune serum from patients with paracoccidioidomycosis and incubated in serum diluted in EDTA-CaCl2. The components C3, and fragments C3c, C3d, C3g, factor H, factor B, C4 and C5b-9 were demonstrated on the Pb18 cell surface by immunofluorescence although no effect was seen on fungal viability.
authors.affiliation: Departamento de Imunologia, Universidade de São Paulo, Brazil.
authors.forename: M E
authors.initials: ME
authors.lastname: Munk
authors.forename: W D
authors.initials: WD
authors.lastname: Da Silva
mesh_terms: D003167:Complement Activation / Q000276:immunology*; D003176:Complement C3 / Q000276:immunology; D006801:Humans; D010228:Paracoccidioides / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000276:immunology
chemical_list: D003176:Complement C3