Morphine determination by gas chromatography/mass spectroscopy in human vitreous humor and comparison with radioimmunoassay.
A gas chromatographic-mass spectroscopic method (GC/MS) for morphine determination in human vitreous humor was developed. Reversed-phase solid-liquid extraction gave better sample purification compared with liquid-liquid or normal-phase solid-liquid extractions. In 10 real cases of death by heroin overdose, the correlation between the results obtained by GC/MS and by radioimmunoassay (RIA) was studied.
Bermejo
A M
AM
Institute of Legal Medicine, Faculty of Medicine, University of Santiago de Compostela, Spain.
Ramos
I
I
Fernández
P
P
López-Rivadulla
M
M
Cruz
A
A
Chiarotti
M
M
Fucci
N
N
Marsilli
R
R
D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009020:Morphine / Q000032:analysis*; D011863:Radioimmunoassay; D014822:Vitreous Body / Q000737:chemistry*
D009020:Morphine