The use of the antiglobulin 'gel-test' for antibody detection.
Four antibodies used routinely in-house for the assessment of antiglobulin reagents (anti-Fyb, anti-Jka, anti-S) were tested in parallel using tube and antiglobulin 'gel-test' low ionic strength antiglobulin techniques. In the latter the red cells are centrifuged following incubation through a dextran matrix incorporating an anti-human globulin reagent. The results show that the antiglobulin 'gel-test' was less sensitive than the tube technique in the detection of these difficult antibodies.
Phillips
P K
PK
National Institute for Biological Standards and Control, South Mimmes, U.K.
Whitton
C M
CM
Lavin
F
F
D000888:Antibodies, Anti-Idiotypic / Q000097:blood*; D001789:Blood Group Antigens; D001788:Blood Grouping and Crossmatching / Q000379:methods; D002498:Centrifugation; D003298:Coombs Test; D005188:False Negative Reactions; D005782:Gels; D006801:Humans; D012015:Reference Standards; D012680:Sensitivity and Specificity
D000888:Antibodies, Anti-Idiotypic; D001789:Blood Group Antigens; D005782:Gels