[Preparation of single-stranded E1A-oncogene DNA fragments from simian adenovirus SA7 by endonuclease hydrolysis recombinant phage M13 SS-DNA complexed with oligonucleotides, containing restriction sites].
The ss-DNA of the (+) and (-) chains of Ela DNA fragment was obtained by hydrolysis of the recombinant bacteriophages M13 mp8G and mp9G (where G is 1-1750 bp:, E1a region of oncogene SA7) in complexes with the 16 bp oligonucleotides containing AluI and BspRI sites of restriction and sequences complementary to E1a SA7. The obtained fragments overlap the E1a zones associated with the immortalizing potential of SA7.
Krutov
A A
AA
Grineva
N I
NI
D017500:Adenovirus E1A Proteins / Q000235:genetics*; D000261:Adenoviruses, Simian / Q000378:metabolism*; D017104:Bacteriophage M13 / Q000235:genetics*; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004279:DNA, Viral / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism*; D015183:Restriction Mapping
D017500:Adenovirus E1A Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009841:Oligonucleotides
Poluchenie odnotiazhevykh fragmentov DNK E1A-onkogena adenovirusa obez'ian SA7 gidrolizom éndonukleazami SS-DNK rekombinantnykh fagov M13 v kompleksakh s oligonukleotidami, soderzhashchimi saĭty restriktsii.