Analysis of large DNA from soybean (Glycine max L. Merr.) by pulsed-field gel electrophoresis.
The technique of pulsed-field gel electrophoresis (PFE) has been used to study chromosomal regions and entire genomes of several organisms. Techniques are presented for the isolation of high molecular weight DNA from embedded soybean protoplasts and the conditions for separating large DNA fragments using PFE. Digestion was detected by Southern hybridization using single copy nodulin clones. These data are being used to generate a physical map of the nodulin region(s) of the soybean genome.
Honeycutt
R J
RJ
Department of Genetics, Iowa State University, Ames 50011.
Sobral
B W
BW
McClelland
M
M
Atherly
A G
AG
D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D017343:Genes, Plant; D008970:Molecular Weight; D010944:Plants / Q000737:chemistry* / Q000235:genetics; D013025:Glycine max / Q000737:chemistry / Q000235:genetics
D004247:DNA