A novel method for blood group typing with the aid of monoclonal antibody immobilized dyed microspheres.
A novel and simple method for blood group typing has been developed. The procedure involves the use of type-specific monoclonal antibodies covalently linked to dyed microspheres of polyglycidyl methacrylate. The presence of ABH and Lewis blood group antigens in saliva and plasma could be determined easily and specifically with respective monoclonal antibodies bound to dyed microspheres. The present method could also be applicable for the determination of the presence of invisible antigens for the forensic and diagnostic purpose with visible agglutination reaction.
Yazawa
S
S
Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Ohkawara
H
H
Nakajima
T
T
Hosomi
O
O
Takeya
A
A
Furukawa
K
K
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D004396:Coloring Agents; D005554:Forensic Medicine; D006801:Humans; D007983:Lewis Blood Group Antigens; D008863:Microspheres; D011102:Polyhydroxyethyl Methacrylate
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D007983:Lewis Blood Group Antigens; D011102:Polyhydroxyethyl Methacrylate