Application of commercially available monoclonal blood grouping reagents to detection of antigens in forensic materials.
Commercial monoclonal reagents for ABO blood grouping were examined for their characteristics in forensic application. In the absorption-elution tests, the monoclonal reagents showed various reactivities, which were generally weaker than polyclonal ones. False negative reactions were observed in the tests for A antigens in some cases of aged or thin bloodstains and hair samples. Each monoclonal reagent showed a limited reactivity to the stains of animal blood. The monoclonal reagents were comparable to polyclonal ones in the absorption characteristics with bloodstains. They showed various reactivities with salivas. In the immunostaining, each of anti-A and anti-B reagent showed a similar staining property against the group antigens of the red cells, vascular endothelia, and various epithelia including the mucous cells of salivary glands.
Maeda
H
H
Department of Legal Medicine, Osaka City University Medical School, Japan.
Imura
M
M
D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal; D005554:Forensic Medicine; D006801:Humans; D007519:Isoantigens / Q000032:analysis*; D011933:Reagent Kits, Diagnostic
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007519:Isoantigens; D011933:Reagent Kits, Diagnostic