[Search for unique segments of the ectromelia virus genome by cross blot hybridization with DNA from other orthopoxviruses].
In order to identify ectromelia virus (EMV) genome regions which may contain genes responsible for the specific pathogenicity of this virus, blot cross-hybridization of EMV DNA with those of other orthopoxviruses was performed. Two hybridization schemes were employed: one of them included hybridization of labelled cloned fragments of EMV with digests of other viral DNAs, the other, reciprocal, consisted in hybridization of labelled total DNAs of various orthopoxviruses with digests of the region of EMV DNA adjacent to the right-terminal inverted repeat. It was demonstrated that the counterpart to an approximately 8-kilobase pair portion of EMV genome flanking the inverted repeat could be detected only in the cowpox virus genome but not in the genomes of vaccinia and rabbitpox viruses. XhoI-O and XhoI-K fragments of EMV DNA contained, along with genes found in other poxviruses, certain genes which appeared to be unique for EMV. It is postulated that some of these genes may determine the specific biological properties of EMV, including its pathogenicity for mice.
Senkevich
T G
TG
Muravnik
G L
GL
Chernos
V I
VI
D004279:DNA, Viral / Q000235:genetics*; D004481:Ectromelia virus / Q000235:genetics* / Q000472:pathogenicity; D016679:Genome, Viral; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011212:Poxviridae / Q000235:genetics*; D014774:Virulence / Q000235:genetics
D004279:DNA, Viral
Poisk unikal'nykh uchastkov genoma virusa éktromelii metodom perekrestnoĭ blot-gibridizatsii s DNK drugikh ortopoksvirusov]