[Survival and the repair of single-stranded DNA breaks in gamma-irradiated Escherichia coli cells adapted to methylmethane sulfonate].
The survival and repair of single-strand breaks of DNA in gamma-ray-irradiated E. coli adapted to MMS (20 mkg/ml during 3 hours) have been investigated. It is shown that the survival of adapted bacteria of radioresistant strains B/r, H/r30, AB1157 and W3110 pol+ increases with DMF (dose modification factor) ranging within 1.4-1.8 and in radiosensitive strains Bs-1, AB1157 recA13 and AB1157 lexA3 with DMF ranging within 1.3-1.4, and does not change in strains with mutation in polA gene P3478 polA1 and 016 res-3. There is no increase in radioresistance during the adaptation to MMS under the action of the protein synthesis inhibitor chloramphenicol. The increase in radioresistance during the adaptation to MMS correlates with the acceleration of repair of gamma-ray-induced single-strand breaks in the radioresistant strains B/r and W3110 pol+ and with the appearance of the ability to repair some part of DNA single-strand breaks in the mutant Bs-1, which beyond the adaptation to MMS does not repair these damages. The incomplete reparability of DNA single-strand breaks in P3478 polA1 strain cells, both adapted and non-adapted to MMS, is equal.
Zhestianikov
V D
VD
Savel'eva
G E
GE
D000222:Adaptation, Physiological / Q000187:drug effects* / Q000528:radiation effects*; D002701:Chloramphenicol / Q000494:pharmacology; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004269:DNA, Bacterial / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D005720:Gamma Rays; D005838:Genotype; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D009154:Mutation / Q000187:drug effects / Q000528:radiation effects; D011836:Radiation Tolerance / Q000187:drug effects
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D002701:Chloramphenicol; D008741:Methyl Methanesulfonate
Vyzhivaemost' i reparatsiia odnonitevykh razryvov DNK v gamma-obluchennykh kletkakh Escherichia coli pri adaptatsii k metilmetansul'fonatu.