title: Distribution of triiodothyronine nuclear receptors during the cell cycle in mouse leukemia cells.
abstract: The process of the appearance of thyroid hormone receptors in the cell cycle was studied in mouse leukemia cells L1210. After cell synchronization by 2 mM thymidine for 12 h followed by 80 nM Colcemid for 4 h, the specific binding of triiodothyronine (T3) to its nuclear receptors was determined 3, 6, 9 and 12 h after release from the thymidine-Colcemid block. Three h after release from the block, [125I]T3 specific binding was 11.4 +/- 2.5% of a control value measured for an asynchronous population. An upward slope in progression of T3 nuclear receptors was found 6 h (32.3% +/- 4.5%), 9 h (47.8% +/- 5.2%) and 12 h (83% +/- 4.3%) after release from thymidine-Colcemid block. The data suggest that processes involving T3 receptor promotion in cell nuclei are operative within the late G1 and S phases of the cell cycle, and thus the increase in T3 receptor concentration in the nuclei is in a positive correlation with the number of cells in the S compartment of the cell cycle.
authors.affiliation: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava.
authors.forename: P
authors.initials: P
authors.lastname: Filipcík
authors.forename: J
authors.initials: J
authors.lastname: Brtko
authors.forename: L
authors.initials: L
authors.lastname: Rauová
authors.forename: V
authors.initials: V
authors.lastname: Sedláková
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D003703:Demecolcine / Q000494:pharmacology; D016193:G1 Phase; D007939:Leukemia L1210 / Q000378:metabolism; D051379:Mice; D011988:Receptors, Thyroid Hormone / Q000378:metabolism*; D016196:S Phase; D013936:Thymidine / Q000494:pharmacology; D014284:Triiodothyronine / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D013936:Thymidine; D003703:Demecolcine