Purification of the neurite outgrowth promoting fragment of mouse laminin.
A method for isolation of the neurite outgrowth promoting fragment of mouse laminin (fragment 8) is described in this paper. Besides producing excellent yields, this method was shown to be fast and practical, since it is based on a single step which consists in an ion exchange chromatography of elastase digested laminin.
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Ludwig Institute for Cancer Research, São Paulo, SP, Brazil.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D007797:Laminin / Q000737:chemistry*; D051379:Mice; D009414:Nerve Growth Factors / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000302:isolation & purification*; D014407:Tumor Cells, Cultured
D007797:Laminin; D009414:Nerve Growth Factors; D010446:Peptide Fragments