Two biochemical indices of mouse bone formation are increased, in vivo, in response to calcitonin.
In a series of four studies, adult female Swiss-Webster mice were used to measure the effects of salmon calcitonin on two biochemical indices of local and systematic bone formation: (1) skeletal alkaline phosphatase activity--in serum and in extracts of calvaria and tibiae, and (2) calvarial collagenase-digestible protein synthesis--measured, acutely, in vitro. Subcutaneous calcitonin doses ranged from 50 to 400 mU/mouse/day (0.95-18.1 U/kg/day), and treatment schedules were continuous (daily) for 2-14 days, acute, or intermittent (2 days/week for 6 weeks). The effects of calcitonin on these bone formation indices (skeletal alkaline phosphatase and collagenase-digestible protein synthesis) were biphasic with respect to dose and treatment time, being increased in response to short-term, low-dose treatment, but not long-term, continuous treatment. The effects of long-term intermittent calcitonin treatment were dose-dependent increases in skeletal alkaline phosphatase in calvaria and serum (r = 0.948, P less than 0.02, and r = 0.960, P less than 0.01, respectively).
Farley
J R
JR
Department of Medicine, Loma Linda University, California.
Hall
S L
SL
Herring
S
S
Tarbaux
N M
NM
D000469:Alkaline Phosphatase / Q000097:blood / Q000378:metabolism*; D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D002116:Calcitonin / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005260:Female; D007279:Injections, Subcutaneous; D051379:Mice; D003012:Microbial Collagenase / Q000378:metabolism
C028815:salmon calcitonin; D002116:Calcitonin; D003094:Collagen; D000469:Alkaline Phosphatase; D003012:Microbial Collagenase