[The radioisotope labelling of thrombocytes using a monoclonal antibody against membrane glycoproteins IIb-IIIa and the measurement of thrombocyte adhesion/aggregation on the substrate surface].
A new method for platelet labeling based on binding of monoclonal antibody to human platelets has been suggested in this study. Monoclonal antibody VM16a against membrane glycoproteins IIb-IIIa was labeled by 125I and then incubated with platelets. About 70% of added antibody was bound when it was used at the concentrations corresponding to the linear part of the concentration curve (0.5 and 1.0 micrograms/ml). Due to high efficiency of binding 125I-VM16a-labeled platelets were used for the measurement of adhesion/aggregation to the substrate in platelet-rich plasma without washing of the free label. Experiments with washed platelets double labeled with 51Cr and 125I-VM 6a showed high correlation between the data obtained with both labels. The method of platelet labeling has been applied for the assessment of drug action on platelet adhesion/aggregation. Measurements were performed in platelet-rich plasma and adhesion/aggregation was stimulated by ADP and analogue of thromboxane A2, U46619. It was shown/that antianginal drug trapidil strongly inhibited and antiatherogenic drug probucol did not affect platelet adhesion/aggregation stimulated by both agonists.
Rainkina
T V
TV
Khorets
B A
BA
Katsenovich
E R
ER
Mazurov
A V
AV
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000000981:diagnostic imaging*; D002860:Chromium Radioisotopes; D004347:Drug Interactions; D006801:Humans; D007457:Iodine Radioisotopes; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010979:Platelet Function Tests / Q000379:methods; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D011341:Probucol / Q000494:pharmacology; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology; D011877:Radionuclide Imaging; D013499:Surface Properties; D014192:Trapidil / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D002860:Chromium Radioisotopes; D007457:Iodine Radioisotopes; D010980:Platelet Membrane Glycoproteins; D011450:Prostaglandin Endoperoxides, Synthetic; D000244:Adenosine Diphosphate; D014192:Trapidil; D011341:Probucol
Radioizotopnoe mechenie trombotsitov s pomoshch'iu monoklonal'nogo antitela protiv membrannykh glikoproteidov IIb-IIIa, izmerenie adgezii/agregatsii trombotsitov na poverkhnosti substrata.