[Usefulness of the ELISA-IgG test in sera and filter paper blood eluates in the Chagas disease immunodiagnosis].
An ELISA-IgG test using a soluble extract of Trypanosoma cruzi antigen has been developed. One hundred and seventy eight sera (74 healthy people, 44 chagasic patients with positive xenodiagnosis and 60 with clinical symptoms), and 64 paper filter blood eluates (35 healthy people, 10 chagasic patients and 19 with clinical symptoms) were tested by ELISA-IgG and an indirect hemagglutination test. Both types of methods presented an overall average concordance of 98.3% and 100.0% with sera and filter paper blood eluates respectively. A cut off (average of optical density plus 3 standard deviations) of 0.365 and 0.379 for sera and eluates was found. The use of ELISA-IgG in Chagas' disease clinical diagnosis and seroepidemiological studies is discussed.
Contreras
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MC
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Chile, Santiago.
Salinas
P
P
Sandoval
L
L
Solís
F
F
Rojas
A
A
D000953:Antigens, Protozoan; D014355:Chagas Disease / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006386:Hemagglutination Tests; D006801:Humans; D007074:Immunoglobulin G
D000953:Antigens, Protozoan; D007074:Immunoglobulin G
Utilidad de la ELISA-IgG en muestras de sueros y eluídos de sangre en papel filtro en el inmunodiagnóstico de la enfermedad de Chagas.