A medium for inducing conversion of Histoplasma capsulatum var. capsulatum into its yeast-like form.
Histoplasma capsulatum var. capsulatum is a dimorphic fungus that, under special conditions, converts from its more common mycelial form to a yeast-like form. Achieving this conversion, however, has been problematical for researchers. The present study tested conversion rates in ten Histoplasma capsulatum var. capsulatum strains using seven culture media, four of which were conventional and three novel. One of our novel media, MLGema, induced complete conversion of two strains within five days of incubation at 35 degrees C, and of all strains that eventually converted by the time of the second subculturing transfer, under defined experimental conditions. MLGema is also inexpensive and easy to produce.
Fressatti
R
R
Departamento de Análises Clínicas, Universidade Estadual de Maringá, PR, Brasil.
Dias-Siqueira
V L
VL
Svidzinski
T I
TI
Herrero
F
F
Kemmelmeier
C
C
D003470:Culture Media; D006658:Histoplasma / Q000254:growth & development*; D009172:Mycology / Q000379:methods*; D013696:Temperature
D003470:Culture Media