title: Isolation of plasma cholesterol-lowering components from ningyotake (Polyporus confluens) mushroom.
abstract: The present study was undertaken to isolate component(s) which contributes to the hypocholesterolemic action of Ningyotake (Polyporus confluens) mushroom. The mushroom powder was extracted with 80% ethanol, and the extract and residue were fractionated into five fractions according to the solubility to solvents. When each fraction was added to a diet containing 1% cholesterol and 0.25% sodium cholate and fed to rats, the plasma cholesterol level was significantly decreased only by ethyl acetate-soluble fraction. Therefore, ethyl acetate-soluble fraction was further fractionated by silica gel column chromatography. Two major compounds, which comprised 45.0% and 28.5% of the ethyl acetate-soluble fraction, were obtained in a pure form by the chromatography, and the compounds were identified as grifolin (2-trans, trans-farnesyl-5-methylresorcinol) and neogrifolin (4-trans, trans-farnesyl-5-methylresorcinol), respectively. The addition of grifolin and neogrifolin to the high cholesterol diet was found to lower plasma cholesterol level significantly.
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sugiyama
authors.forename: H
authors.initials: H
authors.lastname: Kawagishi
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Saeki
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: H
authors.initials: H
authors.lastname: Sakamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Ishiguro
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D005591:Chemical Fractionation; D002784:Cholesterol / Q000032:analysis / Q000097:blood; D008099:Liver / Q000737:chemistry; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011124:Polyporaceae / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012118:Resorcinols / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C000605323:4-farnesyl-5-methylresorcinol; D000924:Anticholesteremic Agents; D010936:Plant Extracts; D012118:Resorcinols; D002784:Cholesterol