[Detection of G-->T mutation at codon 12 of H-ras oncogenes in gastric cancers with polymerase chain reaction and oligodeoxynucleotide(PCR-ODN)].
DNA was extracted from paraffin-embedded gastric cancer tissues, and the possibility of G-->T mutations present at codon 12 of H-ras oncogenes was detected in Chinese gastric cancer patients with polymerase chain reaction (PCR) and oligodeoxynucleotide (ODN) probe hybridization. Point mutations were identified in 5 of 17 gastric cancer cases (29.4%), and in none of 7 gastric ulcer cases. These results indicate that G-->T mutations at codon 12 of H-ras oncogene may play a role in activating the oncogenes in gastric cancers.
Liu
X F
XF
Department of Biochemistry and Molecular Biology, Hubei Medical University, Wuhan.
D001483:Base Sequence; D003062:Codon / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*
D003062:Codon; D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes