[Production of rejuvenated and stable, leukocyte depleted erythrocyte concentrates using the heated centrifugation method].
We report on an alternative to filtration for the preparation of leukocyte-poor red cell concentrates (LP-RCC). It is based on the method of Schneider. Using RCC with buffy coat it is comparably effective in leukocyte reduction [98.3 +/- 1.0%, (3.7 +/- 2.6) x 10(6) leukocytes] and more effective in platelet reduction (96.9 +/- 2.5%). Addition of PAGGS-M before heating (30 min, 37 degrees C) as well as after preparation significantly reduces hemolysis (free hemoglobin, LDH, HBDH) and improves the quality of the LP-RCC (ATP, 2,3-DPG) during storage for 24 h after preparation. LP-RCCs prepared with PAGGS-M after 6-day storage show still better quality than before preparation and about the same quality as LP-RCCs 24 h after conventional preparation with saline solution. In conclusion, by use of PAGGS-M and sterile docking LP-RCCs of adequate quality for 6-day storage can be prepared, improving the supply of the patients concerned.
Kretschmer
V
V
Abteilung Transfusionsmedizin und Gerinnungsphysiologie, Universitätskliniken, Marburg, BRD.
Fauth
F
F
Heins
M
M
Dietrich
G
G
Schulzki
T
T
Bachem
G
G
Brand
O
O
D000225:Adenine; D016913:Blood Component Transfusion / Q000295:instrumentation*; D001793:Blood Preservation / Q000295:instrumentation*; D002498:Centrifugation / Q000295:instrumentation*; D004798:Enzymes / Q000097:blood; D005947:Glucose; D006151:Guanosine; D006361:Heating; D006400:Hematocrit; D006452:Hemoglobinometry; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation*; D007958:Leukocyte Count; D008212:Lymphocyte Depletion / Q000295:instrumentation*; D010710:Phosphates; D010976:Platelet Count; D012965:Sodium Chloride; D013012:Sorbitol
D004798:Enzymes; C053620:PAGGS-sorbitol solution; D010710:Phosphates; D006151:Guanosine; D012965:Sodium Chloride; D013012:Sorbitol; D005947:Glucose; D000225:Adenine
Herstellung rejuvenierter und lagerungsfähiger, leukozytenarmer Erythrozytenkonzentrate mit Hilfe der Anwärm-Zentrifugationsmethode.