[Expression of HIV-1 tat gene under the control of P 7,5 KD vaccinia virus promoter in CV-1 cells].
The transient expression of the HIV-1 gag genes and a HIV-1 ++trans-activator protein (tat)-encoded was made in cultured CV-1 cells. In recombinant plasmids, the gag gene was under the control of HIV-1 ++trans-activator sequence (tar) and the tat gene was under the control of a 7.5-kd vaccinia promoter. Transactivation of gag gene expression, which was stimulated by a tat gene expression product, was observed in the presence of wild vaccinia virus. The transaction was immunologically evaluated from the binding to monoclonal anti-p17 and anti-p24 antibodies. The findings lead to the discussion whether the regulatory proteins of HIV-1 can express in vaccinia virus vectors.
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D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics* / Q000382:microbiology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002144:Callithrix; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D015683:Gene Products, gag / Q000276:immunology; D015756:Genes, tat / Q000235:genetics*; D015488:HIV Antigens / Q000276:immunology; D016655:HIV Core Protein p24 / Q000276:immunology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics*; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
D000911:Antibodies, Monoclonal; D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1
Ekspressiia gena tat VICh-1 pod kontrolem promotora P 7,5 KD virusa ospovaktsiny v CV-1 kletkakh.