A novel general strategy for cloning tumor suppressor genes using radiation-reduced chromosomal superfragments.
To identify the tumor suppressor gene on human chromosome 11p15, we generated mouse microcell hybrids containing small transferable chromosome 11p15 fragments, which we have termed "DNA superfragments". These hybrids will be used to identify which fragments contain a tumor suppressor gene by direct transfer of the fragments to tumor cells via microcell fusion.
Koi
M
M
Howard Hughes Medical Institute, University of Michigan, Ann Arbor.
Johnson
L A
LA
Feinberg
A P
AP
D000818:Animals; D002880:Chromosomes, Human, Pair 11 / Q000528:radiation effects*; D003001:Cloning, Molecular; D016147:Genes, Tumor Suppressor; D006801:Humans; D051379:Mice