title: Altering the insertional specificity of a Drosophila transposable element.
abstract: Vectors derived from the Drosophila P element transposon are widely used to make transgenic Drosophila. Insertion of most P-element-derived vectors is nonrandom, but they exhibit a broad specificity of target sites. During experiments to identify cis-acting regulatory elements of the Drosophila segmentation gene engrailed, we identified a fragment of engrailed DNA that, when included within a P-element vector, strikingly alters the specificity of target sites. P-element vectors that contain this fragment of engrailed regulatory DNA insert at a high frequency near genes expressed in stripes.
authors.affiliation: Laboratory of Cellular and Molecular Biology, Food and Drug Administration, Bethesda, MD 20892.
authors.forename: J A
authors.initials: JA
authors.lastname: Kassis
authors.forename: E
authors.initials: E
authors.lastname: Noll
authors.forename: E P
authors.initials: EP
authors.lastname: VanSickle
authors.forename: W F
authors.initials: WF
authors.lastname: Odenwald
authors.forename: N
authors.initials: N
authors.lastname: Perrimon
mesh_terms: D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005796:Genes; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic
chemical_list: D004251:DNA Transposable Elements; D009838:Oligodeoxyribonucleotides