title: Glucose biosensor using glucose oxidase immobilized in polyaniline.
abstract: A biosensor for glucose utilizing kinetics of glucose oxidase (EC 1.1.3.4.) was developed. The enzyme was immobilized on polyaniline by covalent bonding, using glutaraldehyde as a bifunctional agent. The system showed a linear response up to 2.2 mM of glucose with a response time of 2.5-4.0 min. In addition, the immobilized enzyme had a higher activity between pH 6.5 and 7.5. The system retained 50% of its activity after 30 d of daily use. The optical absorption spectra of the polyaniline/glucose oxidase electrode after glucose had been added to the buffer solution showed that the absorption band around 800 nm had changed considerably when glucose was allowed to react with the electrode. This optical variation makes polyaniline a very promising polymer for use as a support in optical sensor for clinical application.
authors.affiliation: Departamento de Bioquimica, Laboratorio de Imunopatologia, Keizo Asami-LIKA, Brazil.
authors.forename: A H
authors.initials: AH
authors.lastname: Parente
authors.forename: E T
authors.initials: ET
authors.lastname: Marques
authors.forename: W M
authors.initials: WM
authors.lastname: Azevedo
authors.forename: F B
authors.initials: FB
authors.lastname: Diniz
authors.forename: E H
authors.initials: EH
authors.lastname: Melo
authors.forename: J L
authors.initials: JL
authors.lastname: Lima Filho
mesh_terms: D000814:Aniline Compounds; D015374:Biosensing Techniques; D002021:Buffers; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D005976:Glutaral; D011108:Polymers
chemical_list: D000814:Aniline Compounds; D002021:Buffers; D004800:Enzymes, Immobilized; D011108:Polymers; D005949:Glucose Oxidase; D005947:Glucose; C023650:aniline; D005976:Glutaral