Microtiter particle agglutination test for diagnosis of leprosy.
The results of studying the microtiter particle agglutination (MPA) test for detecting anti-Mycobacterium leprae antibodies in blood sera are presented. The serodiagnostic test is based on the agglutination of colored polyacrolein latex microparticles (PAMP) conjugated with 3,6-di-O-methyl-D-glucose (DMG). Sera from 45 leprosy patients (LL, BL), 34 leprosy contacts, and 148 control subjects were investigated by the MPA test. A correlation between the anti-M. leprae antibodies and the bacterial load was found. In many long-treated leprosy patients increased titers of anti-DMG antibodies were observed, which might be due to specific polyneuritis in them. Four contacts of leprosy patients were also positive in the MPA test. "Nonleprosy" sera did not react in the test. The method proposed proved to be of high specificity and sensitivity for the serological diagnosis of leprosy. The rapidity, simplicity, and visual assessment of the results allow the method to be used in the field for epidemiological studies of leprosy contacts and the general population in leprosy-endemic areas.
Dyachina
M N
MN
Leprosy Research Institute, Astrakhan, Russia.
Lukin
Y V
YV
Zubov
V P
VP
Bovin
N V
NV
D019325:3-O-Methylglucose / Q000031:analogs & derivatives*; D000171:Acrolein; D000372:Agglutination Tests / Q000379:methods*; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007918:Leprosy / Q000175:diagnosis*; D008757:Methylglucosides; D008863:Microspheres; D009166:Mycobacterium leprae / Q000276:immunology; D011108:Polymers; D011933:Reagent Kits, Diagnostic
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006017:Glycolipids; D007074:Immunoglobulin G; D008757:Methylglucosides; D011108:Polymers; D011933:Reagent Kits, Diagnostic; C045169:phenolic glycolipid I, Mycobacterium leprae; D019325:3-O-Methylglucose; C029975:polyacrolein; C050112:3,6-di-O-methylglucose; D000171:Acrolein