title: Identification of important bases for the self-cleavage activity at two single-stranded regions of genomic HDV ribozyme.
abstract: In order to determine important bases at two single-stranded regions [SSrA (726-731 nt) and SSrB (762-766)] derived mainly from secondary structure models in genomic hepatitis delta virus (HDV) ribozyme possessing self-cleavage activity, we have constructed several point mutants at these two regions on the HDV88 molecule (683-770). Among the bases at SSrA and SSrB regions C763 was found to play an essential role during self-cleavage process since substitutions to any other bases viz. A or G or U completely abolished the activity.
authors.affiliation: Fermentation Research Institute, Agency of Industrial Science & Technology, MITI, Tsukuba Science City, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nishikawa
authors.forename: Y A
authors.initials: YA
authors.lastname: Suh
authors.forename: P K
authors.initials: PK
authors.lastname: Kumar
authors.forename: J
authors.initials: J
authors.lastname: Kawakami
authors.forename: F
authors.initials: F
authors.lastname: Nishikawa
authors.forename: K
authors.initials: K
authors.lastname: Taira
mesh_terms: D001483:Base Sequence; D003698:Hepatitis Delta Virus / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*
chemical_list: D016337:RNA, Catalytic; D012367:RNA, Viral