title: Neurotensin and neuromedin N stimulate mucin output from human goblet cells (Cl.16E) via neurotensin receptors.
abstract: The stably differentiated human intestinal goblet cell line Cl.16E was used to study the effects of two structurally related regulatory peptides, neurotensin (NT) and neuromedin N (NN), on mucus secretion. NT and NN stimulated rapid release of mucins from filter-grown Cl.16E cells, this effect being dose related with a mean effective dose of 36 nM for NT and 422 nM for NN. The order of potency of NT, three NT fragments corresponding to the NH2-terminal part [NT-(1-11)] or to the COOH-terminal part [NT-(8-13) and NT-(9-13)], and NN in promoting mucin release and in inhibiting 125I-labeled NT binding to Cl.16E cell membranes was identical with NT greater than or equal to NT-(8-13) greater than NN greater than NT-(9-13) much greater than NT-(1-11) supporting the hypothesis that NT and NN stimulate mucin output through interaction with a common NT-preferring receptor. Scatchard analysis of equilibrium binding data showed one population of NT binding sites in Cl.16E cell membranes with the following characteristics: binding capacity (Bmax) was 141 fmol/mg of protein and dissociation constant (Kd) was 1.00 nM.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratoire de Biologie et de Physiologie des Cellules Digestives, U239 Institut National de la Santé et de la Recherche Médicale, Faculté Bichat, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Augeron
authors.forename: T
authors.initials: T
authors.lastname: Voisin
authors.forename: J J
authors.initials: JJ
authors.lastname: Maoret
authors.forename: B
authors.initials: B
authors.lastname: Berthon
authors.forename: M
authors.initials: M
authors.lastname: Laburthe
authors.forename: C L
authors.initials: CL
authors.lastname: Laboisse
mesh_terms: D000595:Amino Acid Sequence; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D003106:Colon / Q000187:drug effects / Q000378:metabolism*; D003110:Colonic Neoplasms; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009077:Mucins / Q000378:metabolism*; D009496:Neurotensin / Q000502:physiology*; D010446:Peptide Fragments / Q000502:physiology*; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
chemical_list: D009077:Mucins; D010446:Peptide Fragments; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter; C042090:neuromedin N; D014660:Vasoactive Intestinal Peptide; D009496:Neurotensin; D002217:Carbachol; D000242:Cyclic AMP; D002118:Calcium