Role of enzymes in tetrahydrobiopterin biosynthesis: computer analysis of the sequential reaction of the last step.
Computer simulation analysis of the experimental data showed that Cl'-keto PH4 and C2'-keto PH4 were sequential intermediates of the reduction of PPH4 to BH4 by SPR and that the reaction in the last step of the BH4 biosynthesis proceeds as follows: PPH4-->Cl'-keto PH4-->C2'-keto PH4-->BH4 Cl'-keto PH4 is not detectable in the reduction of PPH4 by the purified SPR at neutral pH since isomerization (-->) [8] may occur far more rapidly than the first reduction (-->).
Katoh
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S
Deptartment of Biochemistry, Meikai University School of Dentistry, Saitama, Japan.
Sueoka
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T
Hikita
H
H
D000429:Alcohol Oxidoreductases / Q000502:physiology*; D000818:Animals; D001708:Biopterins / Q000031:analogs & derivatives* / Q000096:biosynthesis; D003198:Computer Simulation; D006136:GTP Cyclohydrolase / Q000502:physiology*; D008954:Models, Biological; D019761:Phosphorus-Oxygen Lyases
D001708:Biopterins; D000429:Alcohol Oxidoreductases; C022630:sepiapterin reductase; D006136:GTP Cyclohydrolase; D019761:Phosphorus-Oxygen Lyases; C043497:6-pyruvoyltetrahydropterin synthase; C003402:sapropterin