The effects of adenine and dimethyl sulfoxide on the mouse pancreas.
The authors have studied the effects of dimethyl sulfoxide (DMSO) on the plasma alpha-amylase activity in mice that sustained a pancreatic injury induced by an oral administration of adenine. In mice given a 5% solution of DMSO as drinking water for 3 d prior to the administration of adenine (175 mg/kg), and also drank this DMSO solution until the end of the experiment, hyperemia of the pancreas was observed and the level of plasma alpha-amylase activity became significantly higher than the level seen in the control mice. A pathological examination also revealed vacuolation and zymogenic degranulation. Further, the plasma alpha-amylase activity level increased only in mice given this 5% DMSO solution, and no increase was noted in mice given a 3% or a 1% DMSO solution for drinking water. Further, the pancreatic lipid peroxide level of mice given this 5% DMSO solution was significantly higher than the level seen in the control group. Based on the above results and associated data, it is thought that an oral administration of adenine can induce a pancreatic injury in the mouse, and that this injury is sustained with the assistance of DMSO.
Okazaki
Y
Y
Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.
Minami
T
T
Natsui
K
K
D000225:Adenine / Q000494:pharmacology*; D000284:Administration, Oral; D000681:Amylases / Q000097:blood*; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004347:Drug Interactions; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D051379:Mice; D010179:Pancreas / Q000187:drug effects* / Q000201:enzymology
D001786:Blood Glucose; D008054:Lipid Peroxides; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D000681:Amylases; D000225:Adenine; D004121:Dimethyl Sulfoxide