title: Attachment and orientation of human periodontal ligament fibroblasts to lipopolysaccharide-coated and pathologically altered cementum in vitro.
abstract: Previous studies have suggested that bacterial lipopolysaccharides (LPS) may inhibit fibroblast attachment to root surfaces affected by periodontal disease. This study was carried out to investigate the effects of pathologically altered cementum and cementum coated with LPS on the attachment and orientation of periodontal ligament fibroblasts in vitro. LPS caused a maximum reduction of cell orientation of 26% after 5 days' culture compared with controls. However cells did not become attached and orientated to pathologically altered root surfaces. It was concluded that as yet unidentified factors other than LPS may have greater significance when considering optimal methods for the preparation of root surfaces for new attachment procedures.
authors.affiliation: Department of Oral Medicine and Periodontology, London Hospital Medical College, UK.
authors.forename: F J
authors.initials: FJ
authors.lastname: Hughes
authors.forename: F C
authors.initials: FC
authors.lastname: Smales
mesh_terms: D000704:Analysis of Variance; D002448:Cell Adhesion / Q000502:physiology; D003739:Dental Cementum / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism*; D010513:Periodontal Ligament / Q000502:physiology*; D010514:Periodontal Pocket / Q000503:physiopathology*; D012038:Regeneration; D014092:Tooth Root / Q000503:physiopathology
chemical_list: D008070:Lipopolysaccharides