title: Phospholipase C-mediated hydrolysis of phosphatidylcholine is a target of transforming growth factor beta 1 inhibitory signals.
abstract: Cell growth and tumor transformation can be restrained in certain cell systems by the action of transforming growth factor beta (TGF-beta). It has been established that the mechanism whereby TGF-beta 1 inhibits cell growth does not interfere with the triggering of early mitogenic signal transduction mechanisms. Phospholipase C-catalyzed hydrolysis of phosphatidylcholine (PC) is a relatively late step in the cascade activated by growth factors. Therefore, conceivably activation of phospholipase C-catalyzed hydrolysis of PC could be the target of TGF-beta 1 action. In the study reported here, we demonstrate that TGF-beta 1 inhibits the coupling of ras p21 to the activation of PC hydrolysis, which appears to be critical for the antiproliferative effects of TGF-beta 1.
authors.affiliation: Hospital General Gregorio Marañón, Madrid, Spain.
authors.forename: M T
authors.initials: MT
authors.lastname: Diaz-Meco
authors.forename: I
authors.initials: I
authors.lastname: Dominguez
authors.forename: L
authors.initials: L
authors.lastname: Sanz
authors.forename: M M
authors.initials: MM
authors.lastname: Municio
authors.forename: E
authors.initials: E
authors.lastname: Berra
authors.forename: M E
authors.initials: ME
authors.lastname: Cornet
authors.forename: A
authors.initials: A
authors.lastname: Garcia de Herreros
authors.forename: T
authors.initials: T
authors.lastname: Johansen
authors.forename: J
authors.initials: J
authors.lastname: Moscat
mesh_terms: D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D002460:Cell Line; D004789:Enzyme Activation; D016259:Genes, myc; D006868:Hydrolysis; D007328:Insulin / Q000502:physiology; D015603:Keratinocytes / Q000201:enzymology; D007700:Kinetics; D051379:Mice; D008845:Microinjections; D009865:Oocytes / Q000201:enzymology; D010713:Phosphatidylcholines / Q000378:metabolism*; D011374:Progesterone / Q000502:physiology; D006656:Protamine Kinase / Q000037:antagonists & inhibitors; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000502:physiology*; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D007328:Insulin; D010713:Phosphatidylcholines; D016212:Transforming Growth Factor beta; D011374:Progesterone; D006656:Protamine Kinase; D010738:Type C Phospholipases; D016283:Proto-Oncogene Proteins p21(ras)