Transcription of class II MHC gene by interferon-gamma in FRTL-5 cells.
The intracellular mechanism by which interferon-gamma induces the expression of class II major histocompatibility complex (MHC) antigen in nonlymphoid cells is not clear. The effect of recombinant rat interferon-gamma (IFN-gamma), and cycloheximide on the expression of class II MHC gene was studied using the techniques of immunocytochemical staining and northern blot analysis. IFN-gamma induced de novo transcription of class II MHC gene and class II MHC antigen expression on the cell surface. Cycloheximide did not inhibit IFN-gamma-induced class II MHC antigen expression in a dose-dependent manner indicating translational blockade. These results suggest that IFN-gamma induces class II MHC antigen expression via de novo transcription of class II MHC gene leading to synthesis of new class II MHC molecule.
Lee
M S
MS
Department of Internal Medicine, Korea Cancer Center Hospital, Seoul.
Lee
J O
JO
Kang
T W
TW
Lee
J H
JH
D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005802:Genes, MHC Class II; D007371:Interferon-gamma / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*
D007371:Interferon-gamma; D003513:Cycloheximide