[The possible role of sperm in family HBV infection].
The paper investigated HBV infectious markers in the sperm and spermatozoa of 31 male patients with hepatitis B, in the serum of their 31 spouses and 35 children respectively. HBV markers were found in 12 sperm samples, HBV-DNA was detected in 6 samples of spermatozoa (3 integrated forms and 3 free forms). The samples were hybridized repeatedly with probes of 82P labeled HBV-DNA 82P labeled lambda DNA and 82P labeled pBR822 to exclude false-positive possibility. 8 out of 12 wives (66.67%), were infected whose husband's sperm reacted positively with HBV 8 out of 14 children were infected (57.14%), whose father's sperm had HBV markers, 6 women out of 19 were infected (31.58%) whose husband's sperm did not react with HBV, 11 of 22 children were infected (50%). The investigation found that the HBV in sperm was higher in the patients with HBeAg positive serum. The wives were easier infected from husbands with HBV positive sperm. The result suggested that sperm must be one of medium of HBV transmission. HBsAg HBeAg anti-HBc HBV-DNA were found in the serum sample of one 4 month infant, with no anti-HBc-IgM. His mother was negative in HBV. The integrated form HBV-DNA was detected in his father's spermatozoa. The transmission between father and son is perhaps mainly by contact each other daily. If HBV has the possibility of vertical transmission is to be studied.
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Infectious Department, First Affiliated Hospital Lanzhou Medical College.
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D005192:Family Health; D005260:Female; D006509:Hepatitis B / Q000635:transmission*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D012661:Semen / Q000382:microbiology*; D013094:Spermatozoa / Q000382:microbiology
D004279:DNA, Viral