title: 5838-DNI, a deoxyribonuclease inhibitor produced by Streptomyces sp. strain no. A-5838.
abstract: 5838-DNI, an inhibitor of deoxyribonuclease (DNase) II from porcine spleen was produced by Streptomyces sp. strain No. A-5838. The structure of 5838-DNI was shown to be 1,4,4a,5,12,12a-hexahydro-4,4a,11,12a-tetrahydroxy-3,8-dimethoxy-9- methoxycarbonyl-10-methyl-1,5,12-trioxo naphthacene. Although similar in structure to tetracenomycin C, which is an antibiotic against Gram-positive bacteria, 5838-DNI has different antibacterial activity. 5838-DNI was distinguished from 5923-DNI, a previously reported DNase II inhibitor, in inhibitory activity against each enzyme. 5838-DNI showed dependency of inhibition on pH and temperature, and inhibited phosphodiesterase I in a competitive manner. These data suggest that 5838-DNI is the first reported example of an inhibitor of microbial origin which is able to inhibit DNase II and phosphodiesterase I.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Uyeda
authors.forename: K
authors.initials: K
authors.lastname: Nagao
authors.forename: Y
authors.initials: Y
authors.lastname: Kido
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Hara
authors.forename: R
authors.initials: R
authors.lastname: Yamamura
mesh_terms: D000818:Animals; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015394:Molecular Structure; D009279:Naphthacenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012259:Ribonuclease, Pancreatic / Q000037:antagonists & inhibitors; D013154:Spleen / Q000201:enzymology; D013302:Streptomyces / Q000254:growth & development; D013552:Swine; D013816:Thermodynamics
chemical_list: D004791:Enzyme Inhibitors; D009279:Naphthacenes; C080889:5838 DNI; D003851:Deoxyribonucleases; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II; D012259:Ribonuclease, Pancreatic