title: Vasopressin biosynthesis in rodent Leydig cells.
abstract: Local biosynthesis of the peptide hormone vasopressin is demonstrated in vitro in Leydig cells derived from rat and mouse testis. Cycloheximide-sensitive production of the nonapeptide was shown for rat Leydig cells in primary culture. A polymerase chain reaction technique demonstrated the presence of functionally constituted vasopressin mRNA in rat and mouse testis, primary mouse Leydig cells and in rat and mouse Leydig tumour cell lines (MA10, R2C). Stimulation of cells with gonadotropins, however, had no effect either on peptide production or on levels of specific mRNA. Similarly, treatment of the MA10 cell line with a phorbol ester, or with rat atriopeptin, which activate other second messenger pathways, had no influence on vasopressin mRNA levels. The results are discussed in terms of an autocrine regulatory system which would provide the cell with information about its microenvironment.
authors.affiliation: Institute for Hormone and Fertility Research, Hamburg, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Ivell
authors.forename: N
authors.initials: N
authors.lastname: Hunt
authors.forename: M
authors.initials: M
authors.lastname: Hardy
authors.forename: H
authors.initials: H
authors.lastname: Nicholson
authors.forename: B
authors.initials: B
authors.lastname: Pickering
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology; D001483:Base Sequence; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005246:Feedback; D005786:Gene Expression Regulation / Q000187:drug effects*; D007984:Leydig Cell Tumor / Q000378:metabolism / Q000473:pathology; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015290:Second Messenger Systems / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014667:Vasopressins / Q000096:biosynthesis*
chemical_list: D006063:Chorionic Gonadotropin; D010446:Peptide Fragments; D012333:RNA, Messenger; D014667:Vasopressins; D009320:Atrial Natriuretic Factor; C062524:atrial natriuretic factor prohormone (103-125); D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate