title: Temperature-gradient DNA probe chromatography of nucleic acids on nonporous supports.
abstract: Temperature-gradient DNA probe chromatography of nucleic acids on a nonporous support with a homogeneous particle size of 2.5 microns showed a higher base sequence discriminating power and a larger linear capacity than that on a porous support with a larger and less homogeneous particle size. The resolution on the nonporous support was high enough to separate samples with a single-base mismatch of the less destabilizing groups, including G-G and G-T base mismatches, even when it locates very near the end of the probe.
authors.affiliation: Institute of Physics, College of Arts and Sciences, University of Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Suyama
authors.forename: A
authors.initials: A
authors.lastname: Tsuchiya
authors.forename: M
authors.initials: M
authors.lastname: Fukuda
authors.forename: K
authors.initials: K
authors.lastname: Yano
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D015342:DNA Probes; D009696:Nucleic Acids / Q000032:analysis*; D010316:Particle Size; D013696:Temperature
chemical_list: D015342:DNA Probes; D009696:Nucleic Acids