[Biochemical and immunological characterization of desmosomal proteins].
Desmosmoes were dissolved by incubation at 100 degrees C for 30 minutes in lysis buffer containing 9.5 M urea. SDS-PAGE revealed seven high molecular weight (> 67 kd) bands and some keratins. Seven of these were considered to be major bands. Bands 1 and 2 with M(r) values of 250 kd and 215 kd, called desmoplakins I and II. Polypeptide bands 3, 4a 4b, 5 and 6 had M(r) values of 165kd, 130kd, 115kd, 83kd and 75kd, respectively. 2-2.5mg of Desmoplakin I was obtained by a preparative electrophoresis; the purity reached 93.1%. The isoelectric pH range was between 6.8 and 7.2, and the amino acid compositions displayed a relatively high content of glycine. It was found that McAb Desmoplakin I recognized specifically the 250kd antigenic band by immunoblotting.
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D051180:Desmoplakins; D003896:Desmosomes / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C
D000596:Amino Acids; D003598:Cytoskeletal Proteins; C495294:DSP protein, human; D051180:Desmoplakins; C495295:Dsp protein, mouse