title: Identification of novel adhesion proteins in mouse peritoneal macrophages.
abstract: When mouse peritoneal macrophages were made to adhere firmly on glass surface and then removed by sequential treatment with hypotonic triethanolamine and Nonidet P-40, a set of proteins were found to be left behind at the sites of adherent cells. Such glass-adherent proteins were detected as round or ellipsoidal patches of autofluorescence under a confocal laser microscope, and visualized ultrastructurally as aggregates of narrow threads of unique loop structures which were composed of linearly aligned particles of 22 +/- 2 nm in diameter. Lithium dodecylsulfate-polyacrylamide gel electrophoresis of the glass-adherent proteins showed two major bands, 12 kDa and 14 kDa, which always co-existed in any different sample. The polyclonal antibody raised against these two proteins specifically stained the glass-adherent proteins in situ. The adhesion of macrophages to glass was significantly blocked with Fab fragments of the antibody. The in situ cross-linking experiment suggested that these two proteins might be closely associated with each other to form complexes. Hence, these proteins can be reasonably considered to be responsible for non-specific adhesion of macrophages to glass.
authors.affiliation: Department of Biochemistry, Saitama Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tomita
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000502:physiology; D003432:Cross-Linking Reagents; D005260:Female; D005976:Glutaral; D008264:Macrophages / Q000737:chemistry* / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D010529:Peritoneal Cavity / Q000166:cytology; D013194:Staining and Labeling
chemical_list: D015815:Cell Adhesion Molecules; D003432:Cross-Linking Reagents; D005976:Glutaral