Effect of diethyldithiocarbamate on the activity of nitric oxide-releasing vasodilators.
The effect of diethyldithiocarbamate (DETC, 10(-3) M) on the vasorelaxant activity of acetylcholine, nitric oxide (NO), nitrite, glyceryl trinitrate or dinitrosyl iron cysteine complexes was studied in isolated rat aortic rings contracted with norepinephrine. Pretreatment of these segments with DETC attenuated the vasorelaxation induced by vasodilators and prevented the subsequent restoration of vessel tone, whereas DETC added after the vasodilators induced a rapid restoration of tone. The inhibitory effect of DETC was due to the trapping of NO by a complex of DETC with Fe2+ formed in the tissue.
Vedernikov
Y P
YP
Cardiology Research Center, U.S.S.R. Academy of Medical Sciences, Moscow.
Mordvintcev
P I
PI
Malenkova
I V
IV
Vanin
A F
AF
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000187:drug effects; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D004050:Ditiocarb / Q000494:pharmacology*; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000494:pharmacology*; D009589:Nitrogen Oxides / Q000494:pharmacology; D005996:Nitroglycerin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012977:Sodium Nitrite / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000494:pharmacology*
D009573:Nitrites; D009589:Nitrogen Oxides; D014665:Vasodilator Agents; C072081:dinitrosyl-iron(II)-cysteine; D009569:Nitric Oxide; D004050:Ditiocarb; D005996:Nitroglycerin; D003545:Cysteine; D012977:Sodium Nitrite; D000109:Acetylcholine