title: A 24-amino-acid polypeptide is essential for the biosynthesis of the coenzyme pyrrolo-quinoline-quinone.
abstract: At least four genes are required for the biosynthesis of the coenzyme pyrrolo-quinoline-quinone (PQQ) in Acinetobacter calcoaceticus. The DNA region where one of these genes was mapped codes for a polypeptide of only 24 amino acids. Here we show that indeed this small peptide is essential for PQQ synthesis. Site-directed mutagenesis shows that at least one glutamate and one tyrosine residue of the polypeptide are essential for its function.
authors.affiliation: Department of Molecular Genetics, Gorlaeus Laboratories, University of Leiden, The Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: Goosen
authors.forename: R G
authors.initials: RG
authors.lastname: Huinen
authors.forename: P
authors.initials: P
authors.lastname: van de Putte
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003067:Coenzymes / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D045542:PQQ Cofactor; D015363:Quinolones / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003067:Coenzymes; D015363:Quinolones; D045542:PQQ Cofactor