Expression of the heparin-binding growth factor receptor genes in human megakaryocytic leukemia cells.
K-sam/bek, N-sam/flg and FGFR3/sam3 establish gene family of the receptors for heparin-binding growth factors (HBGFs) or FGFs. These mRNAs were detected in human leukemia cells, CMK, K562 and HEL, which have megakaryocytic phenotype or the potency to differentiate into megakaryocytic lineage. In CMK cells N-sam/flg transcript level was enhanced by the culture with 12-O-tetradecanoylphorbol-13-acetate (TPA). cDNA-polymerase chain reaction identified K-sam/bek mRNA in human platelets, suggesting the involvement of HBGFs in megakaryocytopoiesis and functions of platelets.
Katoh
O
O
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Hattori
Y
Y
Sato
T
T
Kimura
A
A
Kuramoto
A
A
Sugimura
T
T
Terada
M
M
D001483:Base Sequence; D001792:Blood Platelets / Q000378:metabolism; D000091344:Filaggrin Proteins; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007947:Leukemia, Megakaryoblastic, Acute / Q000378:metabolism*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D020794:Receptor Protein-Tyrosine Kinases; D051497:Receptor, Fibroblast Growth Factor, Type 2; D011956:Receptors, Cell Surface / Q000235:genetics; D017468:Receptors, Fibroblast Growth Factor; D011975:Receptors, Mitogen / Q000235:genetics*; D040262:Receptors, Vascular Endothelial Growth Factor; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D011975:Receptors, Mitogen; C496353:FGFR2 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D051497:Receptor, Fibroblast Growth Factor, Type 2; D040262:Receptors, Vascular Endothelial Growth Factor; D013755:Tetradecanoylphorbol Acetate