Nitrobenzo[a]pyrene-induced DNA amplification in SV40-transformed Chinese hamster embryo cells.
Nitrobenzo[a]pyrenes (NBaPs) are ubiquitous environmental pollutants that produce mutations in Salmonella typhimurium and Chinese hamster ovary cells. In this study, 1-, 3-, and 6-NBaP induced amplification of SV40 DNA sequences in an SV40-transformed Chinese hamster embryo cell line which is sensitive to DNA amplification by various known carcinogens. Of the three isomers, 3-NBaP produced the highest level of gene amplification, which was 4.8 relative to untreated controls at a dose of 5 micrograms/ml. Considering the relationship between gene amplification and tumorigenesis, it seems prudent to carry out a more exhaustive analysis of the carcinogenic potential of these agents.
Neft
R E
RE
Division of Genetic Toxicology, Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079-9502.
Roe
A L
AL
Schol
H M
HM
Fu
P P
PP
Mittelstaedt
R A
RA
Casciano
D A
DA
D000818:Animals; D001580:Benzopyrenes / Q000633:toxicity*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004279:DNA, Viral / Q000096:biosynthesis / Q000187:drug effects*; D004622:Embryo, Mammalian / Q000166:cytology; D005784:Gene Amplification / Q000187:drug effects*; D009153:Mutagens; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology
D001580:Benzopyrenes; D004279:DNA, Viral; D009153:Mutagens