The role of growth factor in wound healing.
A pig wound healing model was developed to study the morphological processes involved in incisional and excisional wound healing, the immunohistochemical localization of TGF-alpha in wound healing, and the latter's relevance. In incisional wounds, a few layers of epidermis were regenerated after only 32 hours. In contrast, several layers of epidermis were regenerated on day 7 in excisional wounds. In general, the incisional wound model is useful for evaluating tensile strength, while the excisional wound model is suitable for evaluating epidermal regeneration. Immunohistochemical study showed that keratinocytes migrating from wound edges expressed TGF-alpha very faintly, while TGF-alpha was present prominently in the upper several layers of the epidermis adjacent to the wound, which resembled normal epidermis and showed no changes during the wound healing process.
Higashiyama
M
M
Department of Dermatology, Osaka University School of Medicine, Japan.
Hashimoto
K
K
Takada
A
A
Fujita
K
K
Kido
K
K
Yoshikawa
K
K
D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D012867:Skin / Q000737:chemistry / Q000293:injuries / Q000473:pathology; D013552:Swine; D016212:Transforming Growth Factor beta / Q000032:analysis*; D014945:Wound Healing / Q000502:physiology*
D016212:Transforming Growth Factor beta