[Detection of anti-dsDNA-F(AB')2 antibody and evaluation of its clinical significance].
Purified anti-dsDNA antibody was obtained from the serum of patients with systemic lupus erythematosus by affinity-column chromatography. Anti-dsDNA-F(ab')2 fragment (idiotype) was prepared from digested anti-dsDNA antibody with pepsin. We have developed a sensitive and specific method for detection anti-dsDNA-F(ab')2 antibody. Our result revealed that low titer of anti-dsDNA was observed in patients with active stage of systemic lupus erythematosus.
Kou
Z C
ZC
Department of Medicine, Peking Union Medical College Hospital, Beijing.
Jiang
M
M
Yang
T B
TB
D000328:Adult; D000974:Antibodies, Antinuclear / Q000097:blood* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000097:blood*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*
D000974:Antibodies, Antinuclear; D007140:Immunoglobulin Fab Fragments