title: Demonstration of PGL-I & LAM-B antigens in paraffin sections of leprosy skin lesions.
abstract: An investigation on the demonstration of PGL-I and LAM-B antigens in thirty-four paraffin embedded skin biopsies taken from leprosy patients who covered the whole spectrum of the disease and in four control specimens was carried out. Neither the PGL-I antigen nor the LAM-B antigen was demonstrated in the normal skin specimens that were used as negative control; and only the LAM-B antigen appeared in the tuberculosis specimens in which the PGL-I antigen was negative. The PGL-I antigen was demonstrated on thirty-three leprosy samples except one TT sample and the LAM-B antigen, on all samples by immunochemical staining technique. The antigens were identified as intracytoplasmic bacillary staining, in solitary, granular as well as debris patterns; and as soluble antigenic staining, in vacuolar or amorphous pattern. In LL and BL cases, the antigens were detected predominantly from macrophages and peripheral nerves in all five staining patterns; in BB cases, from macrophages mostly in the granular as well as debris patterns, from the nerves in the vacuolar pattern; while in TT and the majority of BT cases, they were mainly from nerve remnants inside the granuloma in the vacuolar or amorphous staining pattern. In addition, it is interesting to note that the immunochemical staining was able to differentiate the foamy change from the hydropic degeneration. We also found that the antigens distributed in arrector pili muscles and the walls of muscular vessels were obviously related to the unmyelinated nerve fibers innervating the smooth muscle cells.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: National Institute for Leprosy Research, Tokyo.
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Izumi
authors.forename: K I
authors.initials: KI
authors.lastname: Butt
authors.forename: K
authors.initials: K
authors.lastname: Kawatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis*; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007918:Leprosy / Q000175:diagnosis* / Q000473:pathology; D008070:Lipopolysaccharides / Q000032:analysis*; D016612:Paraffin Embedding; D012867:Skin / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D006017:Glycolipids; D008070:Lipopolysaccharides; C050016:lipoarabinomannan; C045169:phenolic glycolipid I, Mycobacterium leprae