[The determination of the genotype of natural reassortant influenza A viruses according to the core protein genes by the methods of competitive dot hybridization and sequencing].
Simultaneous circulation of different subtypes of influenza A viruses provides conditions for reassortant strains formation. A comparative investigation of genome of 47 influenza A virus strains (H1N1, H2N2, and H3N2) was carried out by competitive dot hybridization technique and sequence analysis of some of cDNA-copies of the virus genes. All the genes of 43 strains encoding nonglycolysed proteins corresponded to the serum subtype of surface glycoproteins. The reassortant pattern of genome for some genes of core proteins was revealed in 4 viruses. All the dot hybridization data were completely confirmed by sequence analysis of the genes.
Grinbaum
E B
EB
Zolotarev
F N
FN
Petrov
N A
NA
Litvinova
O M
OM
Konovalenko
I B
IB
Luzianina
T Ia
TIa
Golubev
D B
DB
D000956:Antigens, Viral / Q000235:genetics; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D009980:Influenza A virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D009693:Nucleic Acid Hybridization / Q000379:methods; D010957:Plasmids / Q000235:genetics; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA / Q000379:methods; D012703:Serotyping; D014758:Viral Core Proteins / Q000235:genetics*
D000956:Antigens, Viral; D004279:DNA, Viral; D014758:Viral Core Proteins; D004247:DNA
Opredelenie genotipa prirodnykh reassortantov virusa grippa A po genam vnutrennikh belkov s pomoshch'iu metodov konkurentnoĭ tochechnoĭ gibridizatsii i sekvenirovaniia.