Cytokine-related immunopotentiating activities of paramylon, a beta-(1-->3)-D-glucan from Euglena gracilis.
Paramylon, a beta-(1-->3)-D-glucan, isolated from Euglena gracilis, was tested for its adjuvant activity on the antibody response to sheep red blood cell (SRBC) in mice. Paramylon markedly enhanced anti-SRBC plaque-forming cell production at a dose of 10 mg/kg. It was also found that in vitro addition of lipopolysaccharide in culture to macrophages from paramylon-treated mice produced a large amount of interleukin 1 (IL-1) and there was a significant level of interleukin 6 (IL-6) induced transiently in the blood of these mice. As IL-1 and IL-6 play crucial roles in the immune response to T cell-dependent antigens like SRBC, the immunopotentiating effect of paramylon might be expressed through the action of these cytokines.
Kondo
Y
Y
Pharmaceutical Institute, Tohoku University, Sendai, Japan.
Kato
A
A
Hojo
H
H
Nozoe
S
S
Takeuchi
M
M
Ochi
K
K
D000276:Adjuvants, Immunologic / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D005056:Euglena gracilis / Q000737:chemistry*; D005936:Glucans / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000276:Adjuvants, Immunologic; D005936:Glucans; D007375:Interleukin-1; D015850:Interleukin-6; C003045:paramylon