Identification and characterization of the virion-induced host shutoff product of herpes simplex virus gene UL41.
The virion-induced host shutoff product of the herpes simplex virus UL41 gene is required for shutoff of host translation and degradation of cellular mRNAs. We employed a rabbit antipeptide antiserum to identify a 58K UL41-related phosphoprotein in infected cells. We also provide evidence that this protein is a component of the virus particle, consistent with its role in virion-induced shutoff.
Smibert
C A
CA
Molecular Virology and Immunology Program, McMaster University, Hamilton, Ontario, Canada.
Johnson
D C
DC
Smiley
J R
JR
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D010750:Phosphoproteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D010766:Phosphorylation; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D018139:Simplexvirus / Q000737:chemistry* / Q000235:genetics; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D014771:Virion / Q000737:chemistry* / Q000235:genetics
D010446:Peptide Fragments; D010750:Phosphoproteins; D012333:RNA, Messenger; D014764:Viral Proteins; C064566:herpes simplex virus type 2 protein UL41