An immunoperoxidase method applied to the diagnosis of equine herpesvirus abortion, using conventional and rapid microwave techniques.
An indirect immunoperoxidase (IMP) technique was applied to cryostat and paraffin sections of liver from ten aborted equine foetuses. Equid herpesvirus type 1 (EHV-1) had been isolated from seven of them and EHV-4 from one: the remaining two were virologically negative and were not used as controls. In the eight virus-infected cases the immunostaining revealed foci of cells exhibiting a distinct brown cytoplasmic and inclusion body pigmentation. No specific signal was present in the non-infected controls. The method also was adapted for incubation in a microwave oven, which allowed the total length of the procedure to be reduced to a minimum of 35 mins for cryostat sections, without significant loss of signal.
Whitwell
K E
KE
Department of Infectious Diseases, Animal Health Trust, Newmarket, Suffolk, UK.
Gower
S M
SM
Smith
K C
KC
D000034:Abortion, Veterinary / Q000175:diagnosis*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006564:Herpesviridae / Q000276:immunology / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000276:immunology / Q000302:isolation & purification*; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D007124:Immunoenzyme Techniques; D008099:Liver / Q000196:embryology / Q000382:microbiology / Q000473:pathology; D008872:Microwaves; D009336:Necrosis; D016612:Paraffin Embedding; D011247:Pregnancy
D000956:Antigens, Viral