title: Adjuvant effects of freely ingested cholera toxin on systemic antibody and DTH responses to protein antigen.
abstract: Recent evidence suggests that immunological handling of antigen in the gut may be different when animals voluntarily ingest antigen in their drinking fluid. Therefore, we investigated whether the adjuvant effects of CT were evident in mice that voluntarily ingested CT together with keyhole limpet hemocyanin (KLH) in 1.0 ml chocolate milk. Initially, it was found that ingestion of 2 mg KLH for up to four times (one ingestion per week) did not induce serum IgG anti-KLH antibody; however, KLH-specific IgG was detected if KLH was ingested together with 10 micrograms CT. Furthermore, 50 days after only one ingestion of CT+KLH, the serum IgG response to intraperitoneal challenge with 100 micrograms KLH was significantly elevated with respect to the response of mice who previously had ingested either KLH alone, CT alone, or drinking fluid only. This observation was repeated in further experiments showing an enhanced IgG response to 100 micrograms KLH ip given 7 or 14 days after a single ingestion of 2 or 5 mg KLH mixed with different doses (0.5-20 micrograms) CT. The lowest effective CT dose for serum IgG anti-KLH adjuvanticity was found to be 1 microgram; the highest effective dose was 10 micrograms, there being no additive effect with 20 micrograms CT. It was also found that simultaneous ingestion of KLH (2 or 5 mg) with CT (0.5-20 micrograms) followed 7 or 14 days later by 100 micrograms KLH ip, increased the 24 hr footpad DTH response to 50 micrograms KLH administered 43 days after the ip challenge.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, NY 14642.
authors.forename: A W
authors.initials: AW
authors.lastname: Kusnecov
authors.forename: N
authors.initials: N
authors.lastname: Cohen
authors.forename: J
authors.initials: J
authors.lastname: Moynihan
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D002772:Cholera Toxin / Q000008:administration & dosage*; D004064:Digestive System / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology; D006968:Hypersensitivity, Delayed; D007108:Immune Tolerance; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000276:Adjuvants, Immunologic; D000941:Antigens; D007074:Immunoglobulin G; D002772:Cholera Toxin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin