In vitro inhibition of tumor B cell growth by IgG-BF-producing Fc gamma RII+ T cell hybridoma and by immunoglobulin G-binding factors.
The growth-modulating effect on mouse hybridoma B cells of IgG-BF-producing Fc gamma RII+ mouse T cell hybridomas and of the IgG-BF isolated from the culture supernatants of these cells has been examined. Cocultures of IgG-secreting hybridoma B cells with IgG-BF-producing T hybridomas or with partially purified IgG-BF demonstrated a reproducible inhibition of the tumor B cell growth. The inhibition was due to a cytostatic and not to a cytotoxic effect. Hybridoma B cells cultured in liquid medium in the presence of soluble IgG-BF, or cocultured in semisolid agarose assays with IgG-BF-producing hybridoma T cells did not undergo immediate cytolysis but were prevented from proliferating. Thus, our data indicate that IgG-BF-producing Fc gamma RII+ T cells interfere with the proliferation of transformed B cells, possibly through soluble IgG-BF.
Mathiot
C
C
Laboratoire d'Hématologie, Institut Curie, Paris, France.
Amigorena
S
S
Moncuit
J
J
Sautès
C
C
Fridman
W H
WH
Teillaud
J L
JL
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D002455:Cell Division; D015536:Down-Regulation; D006825:Hybridomas / Q000276:immunology; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D029584:Prostatic Secretory Proteins; D017452:Receptors, IgG / Q000378:metabolism*; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000473:pathology
D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D017452:Receptors, IgG; D013491:Suppressor Factors, Immunologic; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors