Comparative study of adenoviruses with monoclonal antibodies.
The obtainment of monoclonal antibodies for adenovirus species 4(Ad4) is described. The specificities of selected monoclonal antibodies were determined by means of viral neutralization test in cell culture, immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA), in the presence of the following species of human adenovirus: 1, 2, 5 (subgenus C), 4 (subgenus E), 7 and 16 (subgenus B) and 9 (subgenus D). Two monoclonal antibodies species specific to adenovirus 4 (1CIII and 3DIII) and one monoclonal antibody that cross reacted with adenovirus species 4 and 7 (2HIII) were obtained.
de Paiva
T M
TM
Laboratório de Vírus Respiratórios, Instituto Adolfo Lutz, São Paulo, Brasil.
Takimoto
S
S
Ishida
M A
MA
de Souza
M C
MC
Ishimaru
T
T
Neumann
J
J
Kalil
J
J
D000260:Adenoviruses, Human / Q000145:classification / Q000276:immunology*; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000939:Epitopes