title: Acute hypersensitivity to aerosolized histamine induced by aerosolized ovalbumin in guinea pigs.
abstract: Acute airway hyperresponsiveness can be induced after exposure to aerosolized ovalbumin in sensitized guinea pigs. The purpose of the present studies was to determine if "pro-inflammatory agents" would potentiate and prolong antigen-induced pulmonary hyperresponsiveness to histamine in guinea pigs. Guinea pigs were sensitized to aerosolized ovalbumin by exposing them to a 3 min aerosol, generated ultrasonically from a 10% ovalbumin solution on day 0 and day 7. On day 13 the guinea pigs were exposed to a 3 min aerosol of deionized water or a pro-inflammatory agent (1 microgram/ml PAF, 1 mg/ml LPS, or 4% B. pertussis vaccine). Twenty-four hours later, on day 14, the conscious guinea pigs were challenged with a 3 min aerosolized ovalbumin exposure (under isoproterenol cover) and the individual guinea pig responsiveness to aerosolized histamine was determined 2 and 24 h later in an anesthetized modified Konzett-Rossler preparation. Under these experimental conditions, ovalbumin challenge to sensitized guinea pigs produced only an acute hyperresponsiveness (about a 3-10-fold shift) to aerosolized histamine, which lasted less than 24 h. The pro-inflammatory agents neither potentiated nor prolonged the duration of the hyperresponsiveness.
authors.affiliation: Marion Merrell Dow Research Institute, Cincinnati, OH 45215.
authors.forename: T H
authors.initials: TH
authors.lastname: Gieske
authors.forename: L E
authors.initials: LE
authors.lastname: Baugh
mesh_terms: D000336:Aerosols; D000818:Animals; D004417:Dyspnea / Q000139:chemically induced / Q000503:physiopathology; D006168:Guinea Pigs; D006632:Histamine / Q000008:administration & dosage / Q000494:pharmacology*; D007249:Inflammation / Q000139:chemically induced; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D010047:Ovalbumin / Q000276:immunology*; D010567:Pertussis Vaccine / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000276:immunology*
chemical_list: D000336:Aerosols; D008070:Lipopolysaccharides; D010567:Pertussis Vaccine; D006632:Histamine; D010047:Ovalbumin