title: Influences of ginsenosides Rb1, Rb2, and Rb3 on electric and contractile activities of normal and damaged cultured myocardiocytes.
abstract: Free radical damage to the cultured myocardiocytes of Wistar rat was induced by adding xanthine 0.42 mmol.L-1 and xanthine oxidase 5.3 nmol.L-1 to the culture medium. 30 micrograms.ml-1 of Rb1, Rb2 or Rb3 extracted from the leaf and stem of Panax ginseng C A Meyer restored the action potentials (AP) of free radical damaged cells to normal, indicating their antioxidative action. On normal myocardial cells, Rb1, Rb2, Rb3 20 micrograms.ml-1 inhibited the AP and spontaneous contractility, (suggesting the Ca channel blockade action of panaxadiol saponins). The degrees of their inhibitory effects were found to be Rb1 > Rb2 > Rb3. Their effects against X-XO were basically the same.
authors.affiliation: Central Laboratory of Physiology, Norman Bethune University of Medical Sciences, Changchun, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: G G
authors.initials: GG
authors.lastname: Zhong
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: X Y
authors.initials: XY
authors.lastname: Ma
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals; D036145:Ginsenosides; D066298:In Vitro Techniques; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D005894:Panax; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D036145:Ginsenosides; D012503:Saponins; C048026:ginsenoside Rb2; C044463:ginsenoside Rb3