[Preparation of antigen and antibody against aflatoxin B1].
Carboxymethyloxime of aflatoxin B1 (AFB1) was synthesized by refluxing AF B1 with carboxymethoxylamine hemihydrochloride for 4 hours in the presence of pyridine. AF B1-BSA (antigen) was made successfully by coupling AF B1-oxime with bovine serum albumin (BSA) in the presence of a water soluble carbodiimide. The rabbits were immunized with the artificial immunogen which had been purified by dialysis. The titers of antisera were determined by indirect enzyme-linked immunosorbent assay (ELISA) which was developed by ourselves. The highest titer was 1:100,000.
Li
W
W
Lu
C
C
Xiao
Z
Z
Wang
G
G
D016604:Aflatoxin B1 / Q000276:immunology*; D000418:Albumins / Q000276:immunology; D000818:Animals; D000941:Antigens / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000032:analysis*; D011817:Rabbits
D000418:Albumins; D000941:Antigens; D007106:Immune Sera; D016604:Aflatoxin B1