title: Comparative evaluation of the MB-Check system for recovery of mycobacteria from clinical specimens.
abstract: The performance of the MB-Check system (Roche) in the recovery of mycobacteria from clinical specimens was compared with that of the Bactec radiometric system and conventional culture on Löwenstein-Jensen medium. A total of 1,582 clinical specimens were used for the study; 74 yielded mycobacteria. Organisms of the Mycobacterium tuberculosis and Mycobacterium avium complexes accounted for the majority of isolates. The rate of recovery of all mycobacteria with the Bactec system was 91.9%, with the MB-Check system 79.7% and on Löwenstein-Jensen medium only 54.1%. Combination of the Bactec and MB-Check systems allowed recovery of 100% of isolates. The Bactec system showed a faster detection time than the other two methods.
authors.affiliation: Department of Clinical Microbiology, General Hospital Umberto I-Torrette, Ancona, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Piersimoni
authors.forename: V
authors.initials: V
authors.lastname: Morbiducci
authors.forename: G
authors.initials: G
authors.lastname: De Sio
authors.forename: G
authors.initials: G
authors.lastname: Scalise
mesh_terms: D001431:Bacteriological Techniques / Q000295:instrumentation*; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D009161:Mycobacterium / Q000302:isolation & purification*
chemical_list: D003470:Culture Media