Alteration of a DNA-dependent ATPase activity in xeroderma pigmentosum complementation group C cells.
DNA-dependent ATPase activities in crude extracts prepared from HeLa cells were separated into five peaks by fast protein liquid chromatography Mono Q column chromatography. Similar elution profiles were observed with the extracts from human cells normal in repair and xeroderma pigmentosum cells belonging to complementation groups A through G except for group C. An alteration in elution of one of the five ATPases, designated DNA-dependent ATPase Q1, was observed with a cell line of complementation group C. This alteration was observed with all tested cell lines that belonged to group C. ATPase Q1 in HeLa cell extracts exhibited about 2-fold higher activity with ultraviolet light-irradiated DNA as compared to that with non-irradiated DNA, whereas little difference in the effects of two DNAs was observed with the ATPase activities in the extract from group C cells.
Yanagisawa
J
J
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Seki
M
M
Ui
M
M
Enomoto
T
T
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D004265:DNA Helicases / Q000378:metabolism; D005816:Genetic Complementation Test; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D013539:Simian virus 40; D014983:Xeroderma Pigmentosum / Q000235:genetics*
D000251:Adenosine Triphosphatases; D004265:DNA Helicases