title: A monoclonal antibody (IID510g52) for the determination of functional domains within integrin cell surface receptors.
abstract: Monoclonal antibodies against cell surface receptors can be useful for the study of structural and biochemical features involved in protein interactions underlying platelet adhesion and aggregation. We report here the characterization of a monoclonal antibody, IID510g52 (hereafter referred to as IID5), which has been selected based on its specific binding properties against the platelet membrane glycoprotein IIIa. Characterization of the reactive epitope, including evolutionary conservation and identification of related IID5 target antigens in tumor cells, suggest that the IID5 epitope is implicated in the ligand-binding function of integrin receptors. Indeed, we show that this MoAb acts as a potent inhibitor of platelet aggregation and cell adhesion. Taken together, these results indicate that such a monoclonal may be a strategic tool for better understanding multiple integrin-mediated adhesive reactions, as well as the determination of interacting recognition sites.
authors.affiliation: Ludwig Institute for Cancer Research, São Paulo Branch, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Pasqualini
authors.forename: J E
authors.initials: JE
authors.lastname: Levi
authors.forename: M I
authors.initials: MI
authors.lastname: Azul
authors.forename: M
authors.initials: M
authors.lastname: Faria
authors.forename: S J
authors.initials: SJ
authors.lastname: de Souza
authors.forename: R
authors.initials: R
authors.lastname: Brentani
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016824:Antigens, Human Platelet / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology; D002448:Cell Adhesion; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D016023:Integrins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D000951:Antigens, Neoplasm; D000939:Epitopes; D016023:Integrins; D010980:Platelet Membrane Glycoproteins