Fluorescent-labeled oligonucleotides that exhibit a measurable signal in the presence of complementary DNA.
Oligonucleotide derivatives with a fluorescent dye were designed for exhibiting a measurable signal only when they bind to complementary DNA in aqueous solution. The oligonucleotide with a dansyl group at the specific 2'-sugar residue was synthesized by using the protected 2'-dansylaminouridine phosphorobisamidite. The dansyl-oligonucleotide conjugate binds to its complementary DNA to form duplex with a normal stability and exhibits enhanced fluorescence together with a blue-shift in emission maxima after the hybridization. Another possible candidate involved the use of pyrene-excimer emission upon forming ternary complex between two pyrene-labeled oligonucleotide probes with target DNA. A new and general method for introduction of a pyrene fluorophore into the 3'- or 5'-terminal hydroxyl group of oligonucleotides via different linkers was developed.
Yamana
K
K
Department of Applied Chemistry, Himeji Institute of Technology, Japan.
Ohashi
Y
Y
Nunota
K
K
Aoki
M
M
Nakano
H
H
Sangen
O
O
D004247:DNA / Q000032:analysis*; D003619:Dansyl Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000737:chemistry
D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D004247:DNA