[Macronuclear DNA and total protein contents of mating types I and II of Paramecium primaurelia, during the phase of maturity and the transition to senescence. Preliminary observations].
Concerning the studies on mating type differentiation and life cycle development in Paramecium primaurelia stock 90, both macronuclear DNA and total protein contents have been measured cytofluorometrically in mating type I and mating type II isogenic cell lines growing in logarithmic phase, throughout their maturity period and transition to senescence. The target was to investigate whether the two mating types undergo clonal decline in different times, as the previous studies suggested. The results indicate that, throughout the maturity period, macronuclear DNA and total protein contents vary both in mating type I and mating type II cell lines; moreover, aged phenotypes as the dramatic decrease of both contents, firstly occur in mating type II which, therefore, appears to be submitted to clonal decline before mating type I.
Delmonte Corrado
M U
MU
Istituto di Zoologia, Università di Genova.
Crippa Franceschi
T
T
D000375:Aging / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D016054:DNA, Protozoan / Q000032:analysis*; D005434:Flow Cytometry; D010247:Paramecium / Q000737:chemistry / Q000254:growth & development*; D010641:Phenotype; D015800:Protozoan Proteins / Q000032:analysis*; D012098:Reproduction
D016054:DNA, Protozoan; D015800:Protozoan Proteins
Contenuti in DNA macronucleare e in proteine totali dei mating type I e II di Paramecium primaurelia, durante la fase di maturità e la transizione alla senescenza. Osservazioni preliminari.