Quantification of the in vitro activity of some compounds with spermicidal activity.
The in vitro spermicidal activity of the commonly used surfactant spermicides and the antiseptic chlorhexidine, were quantified in a statistically reproducible manner, using donor semen and image capture analysis. The spermicidal activity was expressed as the ED50 under defined assay conditions. Using these parameters, the order of spermicidal activity was: Menfegol > nonoxynol-9 approximately benzalkonium chloride > sodium docusate > chlorhexidine. These differences were statistically significant.
Chantler
E
E
Department of Obstetrics and Gynaecology, University Hospital of South Manchester.
Fisher
H
H
Solanki
S
S
Elstein
M
M
D001548:Benzalkonium Compounds / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D004143:Dioctyl Sulfosuccinic Acid / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D017137:Nonoxynol / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D012044:Regression Analysis; D015203:Reproducibility of Results; D013081:Sperm Motility / Q000187:drug effects; D013089:Spermatocidal Agents / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects*; D013501:Surface-Active Agents / Q000494:pharmacology*; D013997:Time Factors
D001548:Benzalkonium Compounds; D013089:Spermatocidal Agents; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid; D017137:Nonoxynol; D011092:Polyethylene Glycols; C029270:menfegol; C010882:chlorhexidine gluconate; D002710:Chlorhexidine