Use of a yeast site-specific recombinase to generate embryonic mosaics in Drosophila.
An efficient method for generating embryonic mosaics using a yeast site-specific recombinase (FLP), under the control of a heat shock promoter, is described. FLP-recombinase can promote mitotic exchange between homologous chromosomes that contain FRT (FLP Recombination Target) sequences. To demonstrate the efficiency of FLP-recombinase to generate embryonic mosaics, clones of the recessive and cell autonomous mutation armadillo (arm), detected by their ability to differentiate ectopic denticles in the naked cuticle of each abdominal segment, have been induced. We have analyzed the parameters of FLP-recombinase induced embryonic mitotic recombination and have demonstrated that clones can be efficiently induced during the postblastoderm mitotic divisions. We discuss applications of this technique for the analyses of the roles of various mutations during embryonic patterning.
Dang
D T
DT
Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115.
Perrimon
N
N
D000818:Animals; D002999:Clone Cells; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D005260:Female; D006358:Hot Temperature; D008938:Mitosis / Q000235:genetics*; D009030:Mosaicism; D009154:Mutation
D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase