The expression of mouse tyrosinase in chick cells in vitro and in vivo when controlled by a constitutive promoter.
Virally introduced mouse tyrosinase expression was checked both in vitro and in vivo in chicken cells and tissues. The results indicate that a constitutive promoter is able to express mouse tyrosinase in a variety of cells and tissues both in vitro and in vivo. Tyrosinase expression is marked by pigment production in situ, which is visible at macroscopic as well as microscopic levels without the use of substrates. It is concluded that tyrosinase can be a valuable marker for tracking gene insertion since it is spontaneously expressed. The expression of tyrosinase in some cells and tissues has a detrimental effect, however, and should be controlled by tissue-specific promoters.
Frew
T
T
School of Biological Sciences, University of Nebraska, Lincoln 68588-0118.
Whitaker
B
B
Salter
D
D
Payne
W
W
Hughes
S
S
Yamamoto
H
H
Takeuchi
T
T
Brumbaugh
J
J
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D008099:Liver / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure; D008544:Melanocytes / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D014442:Monophenol Monooxygenase / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*
D014442:Monophenol Monooxygenase