title: Effect of LH and prolactin on steroid secretion by perifused luteal tissue from pregnant gilts with induced hypoprolactinemia or after passive immunoneutralization of LH.
abstract: The study was performed using luteal tissue obtained from 24 pregnant gilts. Group I was treated with bromocriptine (BR) from 37th to 42nd day of gestation. Group II was treated with homologous anti-pLH serum from 37th to 42nd day of pregnancy. Group III was given BR from 67th to 72nd day of gestation. Group IV received anti-pLH serum from 67th to 72nd day of pregnancy. The effect of exogenous LH or prolactin (100 ng/ml) on secretion of progesterone (P4) and estradiol (E2) by luteal tissue was studied using perifusion technique. Prolactin caused a significant (P < 0.05) increase in P4 secretion by luteal tissue from gilts in groups I and IV. Both LH and prolactin decreased (P < 0.05) estradiol secretion by luteal tissue obtained from gilts from group IV. Luteinizing hormone stimulated (P < 0.05) P4 and E2 secretion by luteal tissue from gilts from groups IV and II, respectively. The results demonstrate that both LH and prolactin have a regulatory role in steroid secretion by luteal tissue of gilts in the mid- and late periods of pregnancy.
authors.affiliation: Department of Animal Physiology, University of Agriculture and Technology, Olsztyn, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Szafrańska
authors.forename: A
authors.initials: A
authors.lastname: Grazul-Bilska
authors.forename: J
authors.initials: J
authors.lastname: Przała
mesh_terms: D000818:Animals; D001971:Bromocriptine; D003338:Corpus Luteum / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology / Q000502:physiology*; D008659:Metabolic Diseases / Q000139:chemically induced / Q000662:veterinary; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*; D011388:Prolactin / Q000494:pharmacology / Q000502:physiology*; D013552:Swine
chemical_list: D001971:Bromocriptine; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D007986:Luteinizing Hormone