The trophic effect of beta-amyloid 25-35 peptide is not mediated by NK1 or bombesin receptors.
beta-Amyloid peptide and spantide have previously been described to have trophic effects on hippocampal neurones in vitro. We report here that bombesin and [Pro9]-substance P also show a neurotrophic effect on cultured hippocampal neurones. The neurotrophic effect of spantide or a beta-amyloid fragment containing amino acids 25 to 35 was not blocked by addition of the NK1 receptor agonist, substance P or the nonpeptide NK1 antagonist, RP 67580. For the bombesin-related peptides, the antagonist [Tyr4-DPhe12]-bombesin also provokes a trophic response, but the agonist alytesin and the antagonist [Leu13-psi-(CH2NH) Leu14]-bombesin have no effect on neurite growth. These results suggest that the observed trophic responses are unlikely to be mediated by a classical NK1 or bombesin receptor.
Burgevin
M C
MC
Biology Department, Centre de Recherche de Vitry-Alforville, Rhône-Poulenc Rorer, Vitry-sur-Seine, France.
Daniel
N
N
Doble
A
A
Blanchard
J C
JC
D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D001839:Bombesin / Q000031:analogs & derivatives / Q000494:pharmacology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D018004:Receptors, Bombesin; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter / Q000187:drug effects*; D013373:Substance P / Q000494:pharmacology
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D018004:Receptors, Bombesin; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter; C074636:amyloid beta-protein (25-35); D013373:Substance P; D001839:Bombesin