The activity of two immunotoxins composed of monoclonal antibody MoAb-16 and A-chain of ricin (MoAb-16-RTA) or A-chain of mistletoe lectin I (MoAb-16-MLIA).
The A-chains of ricin obtained from Ricinus communis or mistletoe lectin I from Viscum album were coupled to the monoclonal, anti-L1210V antibody MoAb-16, using SPDP as a cross linking agent. The cytotoxic activity in vitro of these immunotoxins was compared. Each of two immunotoxins tested, applied in vitro for 1 h in appropriate doses, caused irreversible inhibition of leukemic L1210 cells proliferation. Unexpectedly, MoAb-16-MLIA immunotoxin appeared to be cytotoxic to normal bone marrow progenitor cells, as observed in NCFUS tests. Moreover, this immunotoxin revealed cytotoxic effect to the P388 leukemia cells which do not share the antigen, common within L1210 leukemia cells, detected by MoAb-16 antibody.
Paprocka
M
M
Department of Tumor Immunology, Polish Academy of Sciences, Wrocław.
Wiedłocha
A
A
Walzel
H
H
Radzikowski
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D000922:Immunotoxins / Q000494:pharmacology*; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000628:therapy; D007941:Leukemia P388 / Q000628:therapy; D051379:Mice; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D012276:Ricin / Q000494:pharmacology*; D014118:Toxins, Biological / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D037102:Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum; D012276:Ricin