title: Cell fusion caused by herpes simplex virus-1 (HSV-1) strains tsB5 and MP is inhibited at pH 6.7 and pH 7.0.
abstract: We investigated the effect of different pH conditions on Vero cell cultures infected with herpes simplex virus-1 (HSV-1) wild-type strain KOS, and syncytial mutants HSV-1 HFEM (tsB5) and HSV-1 mp (MP). Cell fusion was inhibited when infected cells were continuously incubated with culture media adjusted to pH 6.7 or pH 7.0. Inhibition of cell fusion was rapidly reversible when infected cell cultures were returned to pH 7.5. The rate of synthesis and cell-surface expression of virus-specified glycoproteins gB, gC, gD, and gH were not affected during continuous incubation at pH 7.0, but they were reduced at pH 6.7 in comparison to pH 7.5. At later hours p.i. however, these glycoproteins continued to accumulate at all tested pH levels. Accumulation of infectious virions was substantially reduced for MP, KOS, and tsB5 at pH 6.7. At pH 7.0, KOS and tsB5 titers were greatly reduced but MP titers were not affected.
authors.affiliation: Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge.
authors.forename: A
authors.initials: A
authors.lastname: Baghian
authors.forename: M A
authors.initials: MA
authors.lastname: Dietrich
authors.forename: K G
authors.initials: KG
authors.lastname: Kousoulas
mesh_terms: D000818:Animals; D002459:Cell Fusion; D006023:Glycoproteins / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008565:Membrane Proteins / Q000096:biosynthesis; D009154:Mutation; D018139:Simplexvirus / Q000235:genetics / Q000502:physiology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C040495:glycoprotein B, Simplexvirus