Comparison of the X-ray structure of native rubredoxin from Pyrococcus furiosus with the NMR structure of the zinc-substituted protein.
The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at -161 degrees C, and the NMR structure of the zinc-substituted protein, determined in solution at 45 degrees C, are compared. The NMR and X-ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hyperthermostability.
Blake
P R
PR
Department of Chemistry and Biochemistry, University of Maryland Baltimore County 21228.
Day
M W
MW
Hsu
B T
BT
Joshua-Tor
L
L
Park
J B
JB
Hare
D R
DR
Adams
M W
MW
Rees
D C
DC
Summers
M F
MF
D000595:Amino Acid Sequence; D001105:Archaea / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D012416:Rubredoxins / Q000737:chemistry* / Q000378:metabolism*; D014961:X-Ray Diffraction / Q000379:methods; D015032:Zinc / Q000378:metabolism*
D012416:Rubredoxins; D015032:Zinc