[Identification of Mycobacterium tuberculosis and Mycobacterium avium by non-radioactive probes: evaluation of the Snap Syngene system].
We evaluated an alkaline phosphatase-labeled oligonucleotide probe for the rapid identification of Mycobacterium tuberculosis and mycobacteria belonging to the M avium and M intracellulare complex (MAIS). Sixty-two strains of mycobacteria and eight strains belonging to related genera were studied. All M tuberculosis strains hybridized with the tuberculosis probe. All M avium and M intracellulare gave a strong signal with their probes. However the 3 M xenopi strains tested hybridized with all probes for MAIS complex.
Bollet
C
C
Laboratoire de microbiologie et d'hygiène hospitalière, hôpital Salvator, Marseille, France.
Vignoli
C
C
Freney
J
J
Gevaudan
M J
MJ
Galicher
V
V
de Micco
P
P
D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005827:Genetics, Microbial; D066298:In Vitro Techniques; D009162:Mycobacterium avium / Q000235:genetics / Q000302:isolation & purification*; D015269:Mycobacterium avium Complex / Q000235:genetics / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization / Q000379:methods
D015342:DNA Probes; D004269:DNA, Bacterial
Identification de Mycobacterium tuberculosis et des mycobactéries aviaires par sondes non radioactives: évaluation du système Snap Syngène.