Syntheses of oligonucleotide derivatives with P(V) porphyrin and their properties.
Two types of oligonucleotide derivatives which are substituted by P(V) porphyrin at the phosphorus atom of an internucleotidic linkage and at the 5'-terminal internucleotidic linkage via a spacer were synthesized (Fig. 1), and hybridization capabilities of them with complementary oligonucleotides were evaluated. A novel method for a sensing of oligonucleotide by the fluorescence quenching via photo-induced electron transfer between the P(V) porphyrin labeled oligonucleotide and pyrene-labeled one on the oligonucleotide template is reported.
Shimidzu
T
T
Division of Molecular Engineering, Graduate School of Engineering, Kyoto University, Japan.
Segawa
H
H
Kitamura
M
M
Nimura
A
A
D001483:Base Sequence; D004583:Electrons; D005453:Fluorescence; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011166:Porphyrins / Q000737:chemistry*
D009841:Oligonucleotides; D011166:Porphyrins