[The cultivation of Blastocystis (Rhizopoda: Lobosea) from hens and ducks].
The method of cultivation of Blastocystis galli from hens and Blastocystis sp. from ducks was worked out. Blastocystis grow on nutrient medium at pH 7.0 to 7.2 in a wide range of temperatures from 30 to 45 C. Optimum temperatures for cultivation are 41 to 42 C. The growth of cultures was obtained on biphase egg medium. Solid phase of the medium presents coagulated contents of the hen's egg. Liquid phase can be made of Henk's solution with the addition of 30% of fresh or lyophilizinic hen serum or horse serum. Henk's solution can be replaced by medium 199 (we observed the growth of culture on medium 199 without addition of blood serum). In all variants of medium we added antibiotics on a per--1 ml of medium basis: ampicillini--4 thousand units, streptomycini--1 thousand units. After 2 to 3 passages antibiotics can be excluded from the medium. Optimum medium is that with the addition of 30% of fresh hen serum. Passages go well at the transfer of 15-20% culture after 72 to 96 hours. The size of cultural stages varied within the limits of 2.5-56.2 x 2.5-56.2 microns and 2.5-110 x 2.5-110 microns for Blastocystis sp. and B. galli, respectively, the number of nuclei in one individual varied from 1 to 64, seldom over 100.
Belova
L M
LM
D000818:Animals; D016844:Blastocystis / Q000166:cytology / Q000254:growth & development* / Q000302:isolation & purification; D002645:Chickens / Q000469:parasitology*; D003470:Culture Media; D004372:Ducks / Q000469:parasitology*; D005260:Female; D007420:Intestine, Large / Q000469:parasitology; D013696:Temperature; D013997:Time Factors
D003470:Culture Media
Kul'tivirovanie Blastocystis (Rhizopoda: Lobosea) iz kur i utok.