Identification of human, mouse, and rat retinoic acid receptor alpha using monoclonal antibodies.
Monoclonal antibodies that recognize the human, mouse, and rat retinoic acid receptor alpha (RAR alpha) protein have been generated using synthetic peptides. Less well-characterized monoclonal antibodies were also generated against the RAR beta and RAR gamma proteins. Monoclonal antibodies of the IgG1 (R alpha 10) and IgG2a (R alpha 13) isotypes effectively and specifically recognize both the human and mouse RAR alpha protein. Preincubation of the antibodies with the synthetic RAR alpha peptide, but not with the RAR beta or RAR gamma peptides, blocked recognition of the approximately 55 kDa RAR alpha protein on western blots. These monoclonal antibodies also detected differing levels of RAR alpha in various rat tissues. These monoclonal antibodies will serve as powerful reagents to study the structure and regulation of the retinoic acid receptor protein.
Ali
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Gene Expression Laboratory, Pennington Biomedical Research Center, Baton Rouge, Louisiana 70808.
Torian
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BE
Vedeckis
W V
WV
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification; D007132:Immunoglobulin Isotypes; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D018168:Receptors, Retinoic Acid; D012689:Sequence Homology, Nucleic Acid; D014212:Tretinoin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D046911:Macromolecular Substances; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D014212:Tretinoin