Separation and characterization of diastereoisomeric oligonucleotide.
For developing the antisense method, two types of oligonucleotides suitable for antisense molecules were explored for their essential properties. One is oligonucleoside phosphorothioate, and all their possible R/S diastereoisomers were separated and purified by reversed phase liquid chromatography. Isolated diastereoisomers have been investigated for the correlation between their configuration and hybridization manner. Separation, R/S characterization and spectroscopical properties of these oligonucleotides will be discussed.
Murakami
A
A
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Tamura
Y
Y
Nishi
M
M
Morii
T
T
Ide
H
H
Makino
K
K
D002851:Chromatography, High Pressure Liquid; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000302:isolation & purification; D009943:Organophosphorus Compounds / Q000737:chemistry; D013237:Stereoisomerism; D013873:Thionucleotides / Q000737:chemistry
D016376:Oligonucleotides, Antisense; D009943:Organophosphorus Compounds; D013873:Thionucleotides