title: In vitro transfer of N-acetylglucosamine to endogenous glycoprotein acceptors catalyzed by the nucleus and the cytoplasmic membranes prepared from L1210 cells.
abstract: The non-nuclear membranes and the nuclei prepared from L1210 cells catalyze the in vitro transfer of N-acetyl(14C)glucosamine from UDP-N-acetyl(14C)glucosamine to endogenous glycoprotein acceptors. Adequate analysis of these acceptors have demonstrated that the nucleus has its own N-acetylglucosaminyltransferase system that leads to the formation of N-N'-diacetylchitobiosylated proteins.
authors.affiliation: Laboratoire de Biochimie des Membranes, Université Claude Bernard, Lyon, Villeurbanne, France.
authors.forename: J
authors.initials: J
authors.lastname: Frot-Coutaz
authors.forename: A
authors.initials: A
authors.lastname: Degiuli
authors.forename: R
authors.initials: R
authors.lastname: Létoublon
authors.forename: J
authors.initials: J
authors.lastname: Vila
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D002467:Cell Nucleus / Q000201:enzymology*; D004187:Disaccharides; D004586:Electrophoresis; D005936:Glucans / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D007425:Intracellular Membranes / Q000201:enzymology*; D007939:Leukemia L1210; D051379:Mice; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D004187:Disaccharides; D005936:Glucans; D006023:Glycoproteins; D009687:Nuclear Proteins; C010913:N,N-diacetylchitobiose; D000117:Acetylglucosamine