Increased superoxide dismutase activity in aged human cerebrospinal fluid and rat brain determined by electron spin resonance spectrometry using the spin trap method.
Superoxide dismutase (SOD) activity in CSF of patients was determined by electron spin resonance spectrometry using the spin trap method. Variation in SOD activity was found among patients. SOD activity in CSF of subjects increased with age and this was identified as Cu,Zn-SOD activity by electrophoresis. In addition, animal experiments showed that SOD activities were higher in mitochondrial and cytosol fractions of aged rats than in those of adult rats. This finding on aged rat brain validates the increase of SOD activity in aged human CSF.
Hiramatsu
M
M
Yamagata Technopolis Foundation, Japan.
Kohno
M
M
Edamatsu
R
R
Mitsuta
K
K
Mori
A
A
D000375:Aging / Q000134:cerebrospinal fluid*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D003600:Cytosol / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000134:cerebrospinal fluid*
D013482:Superoxide Dismutase