[Neurotensin excited neurons of the dorsal motor vagal nucleus: an in vitro study].
Intracellular recordings were made from neurons of the dorsal motor nucleus of the vagus (DMV) in rat brain slices. Most of DMV neurons (88%) were depolarized by droplets or perfusion of neurotensin (NT) in a dose-dependent manner. The depolarization, accompanied by an increase in membrane resistance, depended on extracellular K+ concentration and reversed polarity at about -82 mV. Perfusion with Ca(2+)-free/high-Mg2+ solution or with 1 mumol/L TTX solution, which blocked synaptic activities, did not eliminate NT-induced depolarization. The results indicate that NT excites DMV neurons through postsynaptic mechanism and this exciting depolarization may result from a decrease in K+ conductance.
Duan
S M
SM
Institute of Navigation Medicine, Nantong Medical College.
Shimizu
N
N
D000818:Animals; D001933:Brain Stem / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*; D009496:Neurotensin / Q000494:pharmacology*; D015221:Potassium Channels / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D014630:Vagus Nerve / Q000166:cytology* / Q000502:physiology
D015221:Potassium Channels; D009496:Neurotensin