[A comparative study of c-sis and c-myc and cellular phenotypes in the arterial media and atherosclerotic plaques].
C-sis and c-myc oncogene probes, random primed DNA labeling with digoxigenin-dUTP and technique of dot blot hybridization by enzyme immunoassay were used to study both c-sis and c-myc gene expression in the arterial media and atherosclerotic plaques collected from human aortae. In addition, the ultrastructure of smooth muscle cells (SMCs) in these specimens was also examined. The results showed that c-sis and c-myc gene expression was increased in the atherosclerotic plaques. The contractile state SMCs and synthetic state SMCs were obtained in the arterial media and the atherosclerotic plaques respectively. The results indicated that different phenotypes of SMCs might give different c-sis and c-myc gene expressions.
Yang
H P
HP
Hengyang Medical College.
D000328:Adult; D001011:Aorta / Q000473:pathology; D001161:Arteriosclerosis / Q000235:genetics* / Q000473:pathology*; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000473:pathology / Q000648:ultrastructure; D010641:Phenotype; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020574:Proto-Oncogene Proteins c-sis; D011519:Proto-Oncogenes
D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis