Recognition of (d(TTTATT) and d(TTATTT) by capillary affinity gel electrophoresis (CAGE) using poly(9-vinyladenine)-polyacrylamide conjugated gel.
Sequence-specific recognition of oligodeoxynucleotide isomers (TTTATT and TTATTT) was achieved by using polyacrylamide-poly(9-vinyladenine) conjugated gel filled capillary affinity gel electrophoresis. It was found that the interaction between poly(9-vinyladenine) and the isomers was dependent on the sequential thymidylic acids of them.
Sawa
T
T
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Japan.
Akashi
M
M
Baba
Y
Y
Tsuhako
M
M
D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000032:analysis* / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry*; D013936:Thymidine / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; D011145:Polyvinyls; C012002:poly(9-vinyladenine); D000225:Adenine; D013936:Thymidine