title: Characterization of the human HOX 7 cDNA and identification of polymorphic markers.
abstract: cDNA clones for a human HOX 7 gene obtained with homologous clones of Drosophila were used in human gene mapping studies. The human cDNA clone was isolated from a library constructed from human embryonic craniofacial material. The sequence of the cDNA demonstrates significant homology with mouse HOX 7. A search for RFLPs identified MboII and BstEII variants. A CA dinucleotide repeat with 5 alleles was also identified and allowed placement of HOX 7 into a defined linkage map. Evidence for linkage disequilibrium was found with markers tested. These results place the human HOX 7 gene in a defined position on 4p.
authors.affiliation: Department of Biology, University of Iowa, Iowa City 52242.
authors.forename: B J
authors.initials: BJ
authors.lastname: Padanilam
authors.forename: H S
authors.initials: HS
authors.lastname: Stadler
authors.forename: K A
authors.initials: KA
authors.lastname: Mills
authors.forename: L B
authors.initials: LB
authors.lastname: McLeod
authors.forename: M
authors.initials: M
authors.lastname: Solursh
authors.forename: B
authors.initials: B
authors.lastname: Lee
authors.forename: F
authors.initials: F
authors.lastname: Ramirez
authors.forename: K H
authors.initials: KH
authors.lastname: Buetow
authors.forename: J C
authors.initials: JC
authors.lastname: Murray
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific; D004330:Drosophila / Q000235:genetics; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005260:Female; D015723:Gene Library; D005801:Genes, Homeobox; D014644:Genetic Variation; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D009838:Oligodeoxyribonucleotides; D012313:RNA; D004247:DNA; C024413:endodeoxyribonuclease MboII; D015252:Deoxyribonucleases, Type II Site-Specific; C032823:GGTNACC-specific type II deoxyribonucleases