Study of 4-NQO induced mutations at the HPRT locus in CHO cell lines. Possible influences of preferential DNA-repair on the mutational spectrum.
In the attempt to determine the possible influence of excision repair processes on 4-NQO mutational spectra in mammalian cells, 4-NQO-induced mutants at the hprt locus were isolated in excision repair proficient (AA8) and deficient (UV5) CHO cell lines. DNA sequencing data on these mutants revealed that DNA repair may indeed modulate the induced mutational spectrum. In particular, more splice mutations were found in the repair proficient than in the repair deficient cells. This can be interpreted by a difference in repairability of the two principal 4-NQO G-adducts or by the existence of a transcription-coupled DNA preferential repair process.
Inga
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A
Laboratorio di Mutagenesi, IST, Università di Genova.
Iannone
R
R
Degan
P
P
Campomenosi
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P
Fronza
G
G
Menichini
P
P
Abbondandolo
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A
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D000818:Animals; D016466:CHO Cells / Q000187:drug effects* / Q000201:enzymology; D006224:Cricetinae; D003412:Cricetulus; D004252:DNA Mutational Analysis; D004260:DNA Repair; D005796:Genes / Q000187:drug effects; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D016296:Mutagenesis; D009152:Mutagenicity Tests; D016133:Polymerase Chain Reaction
D015112:4-Nitroquinoline-1-oxide; D007041:Hypoxanthine Phosphoribosyltransferase