title: Identification and characterization of a new gene in the human Xq28 region.
abstract: A human Xqter chromosome cosmid library was screened with a mixed probe derived from porcine kidney mRNA. A new expressed gene was identified in a cosmid clone known to be part of a G6PD cosmid contig. This gene is most likely a housekeeping gene because the cDNA clone recognizes a 1 kb mRNA transcript in all cell lines and tissues tested. Hybridizing genomic DNA of several species with a cDNA probe indicated that the gene is highly conserved during evolution and that it belongs to a gene family. The genomic sequence shows a 100% homology with the recently identified QM cDNA sequence.
authors.affiliation: Department of Human Genetics, University Hospital of Nijmegen, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: van den Ouweland
authors.forename: P
authors.initials: P
authors.lastname: Kioschis
authors.forename: M
authors.initials: M
authors.lastname: Verdijk
authors.forename: F
authors.initials: F
authors.lastname: Tamanini
authors.forename: D
authors.initials: D
authors.lastname: Toniolo
authors.forename: A
authors.initials: A
authors.lastname: Poustka
authors.forename: B A
authors.initials: BA
authors.lastname: van Oost
mesh_terms: D000326:Adrenoleukodystrophy / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D017124:Conserved Sequence; D003360:Cosmids; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D014960:X Chromosome
chemical_list: D015342:DNA Probes; D004247:DNA