[Evaluation of the method for early pregnancy factor detection (EPF) in swine. Significance in early pregnancy diagnosis].
Early pregnancy factor (EPF) is a protein detected in the serum of pregnant females in most mammals, before implantation of the embryo; its activity is detected by the rosette inhibition test (RIT). After preincubation of lymphocytes in serum of non-pregnant pigs, the mean titre of RIT was 10.80 +/- 0.32. The upper limit of the 95% confidence interval was calculated to be 11.44. Therefore, all titres 12 were defined indicating the presence of EPF activity. The incubation of lymphocytes in serum of pregnancy caused a significant increase in the mean rosette inhibition titre, raising it to 19.88 +/- 1.13 (p < 0.001). False negative results were obtained for 10% of pregnant pigs. EPF positive activity was detected in only one non-pregnant pig (RIT = 20). This assay is not quantitative nor suitable for pregnancy diagnosis taking into account RIT values dispersion.
Greco
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Departamento de Anatomía Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Argentina.
Vivas
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Bosch
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RA
D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology; D018835:Chaperonin 10; D005260:Female; D007166:Immunosuppressive Agents / Q000097:blood*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000502:physiology; D008297:Male; D010455:Peptides / Q000097:blood*; D011247:Pregnancy; D011257:Pregnancy Proteins; D011258:Pregnancy Tests / Q000379:methods*; D012397:Rosette Formation; D013491:Suppressor Factors, Immunologic; D013552:Swine
D000961:Antilymphocyte Serum; D018835:Chaperonin 10; D007166:Immunosuppressive Agents; D010455:Peptides; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic; C025124:early pregnancy factor
Evaluación del método de detección del factor precoz de preñez (EPF) en cerdos. Importancia en el diagnóstico temprano de gestación.