Antithrombin III (AT3) polymorphism in the marsupial Monodelphis domestica: identification and genetics.
Antithrombin III polymorphism was observed in the gray short-tailed opossum, Monodelphis domestica, by either one-dimensional polyacrylamide gel electrophoresis (PAGE; pH 7.9), two-dimensional PAGE (agarose, pH 5.4; 12% T, pH 7.9), or isoelectric focusing (pH 4.2-4.9) followed by immunoblotting with rabbit antiserum to human antithrombin III. Family studies demonstrated an inheritance of three codominant autosomal alleles, AT3A, AT3B, and AT3C, and a population study revealed frequencies of 0.70, 0.10, and 0.20, respectively.
Bell
K
K
Department of Physiology and Pharmacology, University of Queensland, St. Lucia, Australia.
Arthur
H
H
van Oorschot
R A
RA
VandeBerg
J L
JL
D000483:Alleles; D000818:Animals; D000990:Antithrombin III / Q000235:genetics* / Q000302:isolation & purification; D001797:Blood Protein Electrophoresis; D001938:Brazil; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005787:Gene Frequency; D015151:Immunoblotting; D007525:Isoelectric Focusing; D009893:Opossums / Q000097:blood / Q000235:genetics*; D011110:Polymorphism, Genetic
D000990:Antithrombin III