Expression of mouse PDGF-A and PDGF alpha-receptor genes during pre- and post-implantation development: evidence for a developmental shift from an autocrine to a paracrine mode of action.
We examined the expression of platelet-derived growth factor (PDGF)-A and the PDGF alpha-receptor in pre-implantation and early post-implantation mouse embryos. At two-cell and blastocyst stages, all cells express mRNA and protein for both ligand and receptor. In contrast, early post-implantation embryos express PDGF-A chain mRNA in both embryonic ectoderm and in the ectoderm lining the ectoplacental cavity, while mRNA for PDGF alpha-receptor is localized to the mesoderm layers of both embryonic and extra-embryonic membranes. At days 3.5 and 7.5, receptors are demonstrably functional in response to exogenous PDGF-AA. We propose that chronic autostimulation of PDGF alpha-receptors occurs in pre-implantation embryos, whereas, following implantation, early mesoderm development is dependent on stimulation by ectodermally produced PDGF-A.
Palmieri
S L
SL
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, MA.
Payne
J
J
Stiles
C D
CD
Biggers
J D
JD
Mercola
M
M
D000818:Animals; D004475:Ectoderm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D005796:Genes / Q000502:physiology; D017403:In Situ Hybridization; D008648:Mesoderm / Q000378:metabolism; D051379:Mice / Q000196:embryology*; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D011247:Pregnancy; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D017479:Receptors, Platelet-Derived Growth Factor / Q000096:biosynthesis*; D014158:Transcription, Genetic
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; C075278:platelet-derived growth factor A; D011505:Protein-Tyrosine Kinases; D017479:Receptors, Platelet-Derived Growth Factor