Production of cephalexin by Gluconobacter oxydans CCRC 10383.
Intact cells of Gluconobacter oxydans CCRC 10383 produced cephalexin from 7-amino-3-deacetoxy cephalosporanic acid (7-ADCA) and D-alpha-phenylglycine methylester HC1 (PGM). Factors affecting the production of cephalexin by G. oxydans CCRC 10383 were studied. The optimum pH and temperature for the synthetic reaction of cephalexin were 6.0 and 42 degrees C, respectively. A higher concentration of PGM than 7-ADCA was required to obtain a good yield of cephalexin.
Liu
Y T
YT
Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Lee
S H
SH
D000091:Acetobacter / Q000378:metabolism; D016947:Acetobacteraceae / Q000378:metabolism*; D002506:Cephalexin / Q000378:metabolism*; D002511:Cephalosporins / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives / Q000378:metabolism; D016958:Pseudomonas putida / Q000378:metabolism; D014974:Xanthomonas / Q000378:metabolism
D002511:Cephalosporins; C033393:methyl phenylglycine; C014320:7-aminodesacetoxycephalosporanic acid; D002506:Cephalexin; D005998:Glycine