title: Protein kinase C (alpha, beta, gamma) in Pacinian corpuscle.
abstract: Immunocytochemical demonstration of protein kinase C (PKC) subspecies (alpha, beta, gamma) was carried out in Pacinian corpuscles of rat hind feet using monoclonal or polyclonal antibodies against each of these subspecies. The inner core cells and lamellae and the Schwann cell cytoplasm of the nerve fiber innervating the corpuscle were strongly positive for PKC alpha-immunoreactivity (IR). In contrast, the axon terminal and the outer core did not display any positive alpha-IR. Very weak PKC beta-IR was detected in the ultraterminal region of the axon terminal, while the trunk region showed no immunoreactivity. Very faint PKC beta-IR was found also in the lamellar cells located at the periphery of the inner core and the endoneurial fibroblasts in the intermediate layer. PKC gamma-IR was not detected in any part of the corpuscle. The strong PKC alpha-IR in the inner core and the presence or absence of PKC alpha-, beta-, and gamma-IR in the axon terminal are discussed from the point of view of the functional aspects of each part.
authors.affiliation: Department of Anatomy, Kobe University School of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kawakita
authors.forename: A
authors.initials: A
authors.lastname: Mizoguchi
authors.forename: M
authors.initials: M
authors.lastname: Masutani
authors.forename: M
authors.initials: M
authors.lastname: Arakawa
authors.forename: C
authors.initials: C
authors.lastname: Ide
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003593:Cytoplasm / Q000201:enzymology / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D010141:Pacinian Corpuscles / Q000201:enzymology* / Q000648:ultrastructure; D011493:Protein Kinase C / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000911:Antibodies, Monoclonal; D011493:Protein Kinase C