[The use of sodium azide as a fecal preservative for studying the eggs of Schistosoma mansoni and of other helminths by the Kato-Katz method: a study in an endemic area].
In two aliquots of 208 samples of stool, tests have been made by the Kato-Katz method, one being made after the use of 0.2mg of sodic azide for 200mg, and the other without the aforesaid conservant. The resulted percentages with or without the conservant were, respectively, for Ancylostomideos: 12.5 and 25.9; for Ascaris lumbricoides: 71.6 and 72.5; for Schistosoma mansoni: 7.6 and 17.7, and for Trichuris trichiura: 86 and 85. The count of the eggs with and without the conservant was, respectively, 264 and 539 for Ancylostomideos, 13186 and 33751 for A. lumbricoides, 55.5 and 63.5 for S. mansoni, and 1345 and 2068 for T. trichiura. The authors did not confirm the advantage of using sodic azide for study in endemic areas. They suggest that the exsiccation of the stool and the low intensity of infections can explain the unfavourable results of the present clinical trial.
Amato Neto
V
V
Laboratório de Investigação Médica/Parasitologia, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo.
Pinto
P L
PL
Torres
O D
OD
Matsubara
L
L
Braz
L M
LM
do Nascimento
S A
SA
D000818:Animals; D001386:Azides; D001938:Brazil; D004197:Disease Reservoirs; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology*; D006376:Helminths; D006801:Humans; D007202:Indicators and Reagents; D010270:Parasite Egg Count / Q000379:methods; D011309:Preservation, Biological / Q000379:methods*; D012550:Schistosoma mansoni; D019810:Sodium Azide
D001386:Azides; D007202:Indicators and Reagents; D019810:Sodium Azide
Emprego da azida sódica, como conservador de fezes, para a pesquisa de ovos de Schistosoma mansoni e de outros helmintos, pelo método de Kato-Katz: estudo em área endêmica.