title: A 17.6 kbp region located upstream of the rabbit WAP gene directs high level expression of a functional human protein variant in transgenic mouse milk.
abstract: We have investigated whether DNA regions present in the rabbit whey acidic protein (WAP) promoter/5' flanking sequence could potentially confer, in vivo, high level expression of reporter genes. Transgenic mice were generated expressing a variant of human alpha 1-antitrypsin, which has inhibitory activity against plasma kallikrein under the control of a 17.6 kbp DNA fragment located upstream of the rabbit WAP gene. Up to 10 mg/ml of active and correctly processed recombinant protein were detected in mouse milk, thus suggesting that the far upstream DNA sequences from the rabbit WAP gene might be useful for engineering efficient protein production in the mammary glands of transgenic animals.
authors.affiliation: TRANSGENE S.A., Strasbourg, France.
authors.forename: R
authors.initials: R
authors.lastname: Bischoff
authors.forename: E
authors.initials: E
authors.lastname: Degryse
authors.forename: F
authors.initials: F
authors.lastname: Perraud
authors.forename: W
authors.initials: W
authors.lastname: Dalemans
authors.forename: D
authors.initials: D
authors.lastname: Ali-Hadji
authors.forename: D
authors.initials: D
authors.lastname: Thépot
authors.forename: E
authors.initials: E
authors.lastname: Devinoy
authors.forename: L M
authors.initials: LM
authors.lastname: Houdebine
authors.forename: A
authors.initials: A
authors.lastname: Pavirani
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000378:metabolism; D008894:Milk Proteins / Q000235:genetics*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology*; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D008894:Milk Proteins; D011993:Recombinant Fusion Proteins; D000515:alpha 1-Antitrypsin; C024471:whey acidic proteins