title: Absence of direct regulation of prolactin cells by estradiol-17 beta in rainbow trout (Oncorhynchus mykiss).
abstract: The effects of estradiol-17 beta (E2) implants on plasma prolactin (PRL) concentrations, pituitary PRL content and pituitary PRL mRNA levels were examined in rainbow trout (Oncorhynchus mykiss). Intact immature fish treated with 1 mg estradiol-17 beta did not show significant changes in both PRL mRNA levels and pituitary PRL content after 3 days of treatment. In a similar experiment, no changes were observed in plasma PRL levels followed during 7 days. Similarly, lack of estradiol-17 beta effect on plasma PRL levels and on final PRL pituitary content was observed in ovariectomized female rainbow trout treated during 48 days with 25 mg estradiol-17 beta and in mature male fish over a 3-week treatment period. Localization of estradiol receptor (ER) mRNAs in the pituitary was carried out by Northern blot analysis using a full-length rainbow trout estrogen receptor (rtER) cDNA as a probe. The rostral pars distalis of the pituitary which contained mostly PRL cells showed the lower amount of rtER mRNA when compared to other parts of the pituitary. Moreover, two mRNAs of different size (3.5 and 1.4 kb) were detected in different parts of the pituitary. Further hybridization experiments using probes containing part of the rtER cDNA (E domain or C and D domains) indicated that the small-sized mRNA (1.4 kb) probably encodes a truncated ER protein lacking hormone binding domain or an ER-related protein. Thus, only the 3.56 kb mRNA appeared to be involved in the regulation of pituitary function by estradiol. In situ hybridization analysis allowed a more precise localization of this rtER mRNA in the pituitary.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratoire de Biologie Moléculaire, U.R.A. 256 C.N.R.S., Université de Rennes I, France.
authors.forename: P
authors.initials: P
authors.lastname: Le Goff
authors.forename: G
authors.initials: G
authors.lastname: Salbert
authors.forename: P
authors.initials: P
authors.lastname: Prunet
authors.forename: C
authors.initials: C
authors.lastname: Saligaut
authors.forename: B T
authors.initials: BT
authors.lastname: Bjornsson
authors.forename: C
authors.initials: C
authors.lastname: Haux
authors.forename: Y
authors.initials: Y
authors.lastname: Valotaire
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004958:Estradiol / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D017403:In Situ Hybridization; D008297:Male; D010052:Ovariectomy; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D011959:Receptors, Estradiol / Q000032:analysis; D012636:Secretory Rate / Q000187:drug effects; D014337:Trout / Q000254:growth & development / Q000502:physiology*
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D011959:Receptors, Estradiol; D004958:Estradiol; D011388:Prolactin; D004247:DNA