[Studies on metabolism of fungicide benzoic acid, 1,3-dithiolan-2-ylidenehydrazide in vitro].
The metabolism of fungicide benzoic acid, 1,3-dithiolan-2-ylidenehydrazied (Yekuling) was studied quantitatively in rat liver microsomes and liver soluble fractions pretreated with phenobarbital (PB) and 3-methylcholanthrene (3-MC) by high pressure liquid chromatography. The experimental results indicated that the major metabolic pathway of Yekuling in vitro was hydrolysis. PB can enhance amidase activity to increase formation of benzoic acid and 1,3-dithiolan-2-ylidenehydrazine. 3-MC treatment elevated rat liver microsomal cytochrome P-448, enhancing S-oxidation of Yekuling. On the other hand, S-oxidation of Yekuling by rat liver microsomal MFO was NADPH-dependent.
Gao
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Department of Environmental Health, Third Military Medical College, Chongqing.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005659:Fungicides, Industrial / Q000378:metabolism*; D006835:Hydrazones / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010575:Pesticides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D005659:Fungicides, Industrial; D006835:Hydrazones; D010575:Pesticides; C049780:2-benzoylhydrazono-1,3-dithiolane