Herpes simplex virus type 2 and pseudorabies virus associated growth factors and their role in the latency in vitro.
A putative herpes simplex virus type 2 (HSV-2) growth factor (HSGF-2) was detected in a crude extract from virus infected mouse embryo cells. This factor, similar to previously described pseudorabies virus (PRV) associated growth factor (PRGF) was shown to have ability to morphologically transform non-transformed cells and to repress the transformed phenotype of transformed cells. Both activities could be neutralized with two, out of seven monoclonal antibodies directed against glycoprotein B of HSV-2. Both PRGF and HSGF-2 were detected in human embryo lung cells latently infected with PRV or HSV-2 either at 41 degrees C, or in the presence of phosphonoacetic acid. Human alpha-2 interferon, when present in medium of latently infected cells enhanced the production of both HSGF and PRGF. On the contrary, when latently infected cells were treated with 5-azacytidine the synthesis of both PRGF and HSGF-2 was completely blocked and the virus reactivated from latency replicated to higher titers than in non-treated cells. The role of PRGF and HSGF-2 in the establishment, maintenance and reactivation of latency, as well as in cellular transformation is discussed.
Golais
F
F
Comenius University, Department of Microbiology and Virology, Bratislava, Czechoslovakia.
Csabayová
M
M
Lesko
J
J
Bystrická
M
M
Sabó
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D001374:Azacitidine / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D004622:Embryo, Mammalian; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology / Q000502:physiology; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology / Q000502:physiology; D006367:HeLa Cells / Q000187:drug effects; D011558:Herpesvirus 1, Suid / Q000502:physiology*; D006801:Humans; D051379:Mice; D009500:Neutralization Tests; D010641:Phenotype; D018139:Simplexvirus / Q000276:immunology / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D014775:Virus Activation
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D006023:Glycoproteins; D006133:Growth Substances; D014759:Viral Envelope Proteins; C044525:glycoprotein B, herpes simplex virus type 2; D001374:Azacitidine