title: Recombinant antigens in viral diagnosis.
abstract: DNA fragments coding for a variety of different viral antigens have been cloned and expressed in Escherichia coli. Selected purified recombinant antigens were used for detection of specific antibodies by means of the ELISA technique. This approach has been used for the development of four different ELISAs for the detection of HIV- and EBV-specific antibodies.
authors.affiliation: Biotest AG, Research Department, Dreieichenhain, FRG.
authors.forename: R
authors.initials: R
authors.lastname: Vornhagen
authors.forename: A
authors.initials: A
authors.lastname: Baur
authors.forename: G
authors.initials: G
authors.lastname: Jahn
authors.forename: W
authors.initials: W
authors.lastname: Hinderer
authors.forename: H
authors.initials: H
authors.lastname: Nebel-Schickel
authors.forename: J
authors.initials: J
authors.lastname: Horn
authors.forename: H
authors.initials: H
authors.lastname: Wolf
authors.forename: H H
authors.initials: HH
authors.lastname: Sonneborn
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D015483:HIV Antibodies / Q000032:analysis*; D015497:HIV-1 / Q000276:immunology; D015498:HIV-2 / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D011994:Recombinant Proteins
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D015483:HIV Antibodies; D011994:Recombinant Proteins