Determination on the microscale of plasmatic lactic acid as its t-butyldimethylsilyl derivative by stable-isotope dilution using capillary gas chromatography/mass spectrometry.
A new sensitive and precise method for the determination of lactic acid in plasmatic microsamples (50 microL) has been developed. Lactic acid was directly extracted from plasma by ethyl acetate in acidic conditions, and analysed as its di-t-butyldimethylsilyl derivative by capillary gas chromatography/electron-impact mass spectrometry (GC/MS). The internal standard was a previously synthesized deuterated compound, 3-[2H]-(2R)-lactic acid. The method gives good reproductibility and precision, the overall standard deviation being better than 3%. The GC/MS assay was in good agreement with the enzymatic determination.
Rocchiccioli
F
F
INSERM U 342, Hôpital Saint-Vincent-de-Paul, Paris, France.
Sanière
M
M
D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006868:Hydrolysis; D007202:Indicators and Reagents; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D017646:Organosilicon Compounds; D011865:Radioisotope Dilution Technique; D012825:Silicon
D007202:Indicators and Reagents; D007773:Lactates; D017646:Organosilicon Compounds; C009660:t-butyldimethylsilyl compounds; D019344:Lactic Acid; D012825:Silicon