title: Does RNA hydrolysis involve rate-limiting formation of the pentacoordinate intermediate or rate-limiting decomposition? Phenyl ester of adenosine 3'-phosphate as a novel probe.
abstract: The rate-limiting step of enzymatic and non-enzymatic hydrolysis of RNA has been experimentally determined by use of phenyl ester of adenosine 3'-phosphate as a novel probe. Alkaline hydrolysis of RNA involves rate-limiting decomposition of a pentacoordinate intermediate, and any catalyst, either enzymatic or non-enzymatic, should promote the decomposition step.
authors.affiliation: Institute of Materials Science, University of Tsukuba, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shiiba
authors.forename: M
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000737:chemistry; D002384:Catalysis; D006868:Hydrolysis; D007700:Kinetics; D015335:Molecular Probes; D012313:RNA / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; C079752:adenosine 3'-phosphate phenyl ester; D000249:Adenosine Monophosphate; D012313:RNA