title: [The immunochemical and biological properties of Leptospira membranes].
abstract: The immunochemical and biological properties of purified membrane fractions obtained from Leptospira interrogans, serovar copenhageni, strain Rat 2, and Leptospira biflexa, strain Patoc 1, were studied. The presence of genus-specific and group-specific antigens in leptospiral membranes was established by the methods of immunodiffusion analysis, the microagglutination (MA) and lysis tests. In animal experiments cell membrane preparations produced no toxic and allergic effects. Leptospiral membranes obtained from strain Rat 2 ensured the protection of golden hamsters infected with Leptospira virulent culture and induced antibody production in high titers, detected with the use of the MA test, the lysis test and the enzyme immunoassay, in rabbits immunized in two injections.
authors.forename: B F
authors.initials: BF
authors.lastname: Vachaev
authors.forename: E A
authors.initials: EA
authors.lastname: Iagovkin
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001428:Bacterial Vaccines / Q000276:immunology; D002458:Cell Fractionation; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D006224:Cricetinae; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007114:Immunization; D007921:Leptospira interrogans / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure; D008647:Mesocricetus; D051379:Mice; D008854:Microscopy, Electron; D051381:Rats; D012703:Serotyping; D012995:Solubility
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines
vernacular_title: Immunokhimicheskie i biologicheskie svoĭstva mebran leptospir.