[The cloning and expression of the RNAse structural gene of Bacillus intermedius].
The structure gene of extracellular alkaline ribonuclease Bacillus intermedius (binase) has been cloned in E. coli cells in composition of pMT 316 plasmid carrying the inhibitor gene (barstar of barnase--binase structure homologue. The possibility to use such vector has been proved during the barstar action on binase catalytic activity. Using biochemical immunochemical analysis the expression of binase gene in E. coli cells has been confirmed. The recombinant clone E. coli which contains both plasmids simultaneously--carrying gene for barster and for benase has been produced. The given vector is suggested to be used for cloning of inhibitor gene to obtain a viable producer of alkaline intracellular ribonuclease.
Chernokal'skaia
E B
EB
Romakhina
E R
ER
Balaban
N P
NP
Sharipova
M R
MR
Vershinina
V I
VI
Leshchinskaia
I B
IB
D001407:Bacillus / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004722:Endoribonucleases / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005095:Exoribonucleases / Q000032:analysis / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012260:Ribonucleases / Q000032:analysis / Q000235:genetics
D001426:Bacterial Proteins; C057438:barstar protein, Bacillus amyloliquefaciens; D004722:Endoribonucleases; D005095:Exoribonucleases; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease; C022543:ribonuclease T(2)
Klonirovanie i ékspressiia strukturnogo gena RNKazy Bacillus intermedius.