Calcium is required for the mitogenic activation of lymphocytes by periodic acid oxidation.
This investigation of Ca2+ requirements for the mitogenic activation of lymphocytes by periodic acid has shown that oxidation by periodate causes an immediate and transient increase of Ca2+ influx and efflux in oxidized cells. Oxidized lymphocytes maintained in the medium containing 0.2 mM Ca2+ failed to proliferate or to produce IL-2, whereas a 1.4 mM Ca2+ concentration was shown to be sufficient to sustain cellular proliferation and IL-2 secretion. These results indicate that mitogenic activation of lymphocytes by periodic acid oxidation is Ca(2+)-dependent.
Hornicek
F J
FJ
Department of Orthopaedics and Rehabilitation, University of Miami School of Medicine, FL 33101.
Malinin
G I
GI
Malinin
T I
TI
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000502:physiology; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D007376:Interleukin-2; D010504:Periodic Acid; D002118:Calcium