Structure of G-CSF: significance of the sugar chain.
1. The carbohydrate chain protects rhG-CSF from polymerization and/or conformational alterations associated with physicochemical changes, elevation of pH or temperature fluctuations. 2. The carbohydrate chain of rhG-CSF prevents loss of its biological activity in normal human serum by inhibiting proteinase activity. 3. These facts indicate that the carbohydrate chain of rhG-CSF has a markedly important role in maintaining the stability of the protein itself as well as in effecting the exertion of its biological activity.
Ono
M
M
Central Research Laboratories, Chugai Pharmaceutical Co., Ltd., Tokyo, Japan.
Oh-eda
M
M
Kamachi
S
S
Kato
M
M
Endo
Y
Y
Ochi
N
N
D002241:Carbohydrates / Q000737:chemistry*; D003114:Colony-Forming Units Assay; D004355:Drug Stability; D004926:Escherichia coli / Q000737:chemistry; D006031:Glycosylation; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011994:Recombinant Proteins / Q000737:chemistry
D002241:Carbohydrates; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor