title: In vitro keratin expression of hair cells.
abstract: Human hair follicles were isolated from the scalp by dispase and collagenase treatment and dispersed into a cell suspension by trypsin. These cells proliferated well and could be subcultured 7 to 8 times. The medium used was MCDB 153 HAA medium further supplemented with some amino acids, hydrocortisone, insulin, EGF, and bovine brain extract. The concentration of Ca++ was adjusted to 0.1 mM. Immunohistochemically, these cells were proved to possess keratins specific to hair forming cells.
authors.affiliation: Department of Dermatology, Hyogo College of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kitano
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: E
authors.initials: E
authors.lastname: Okamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Okano
authors.forename: N
authors.initials: N
authors.lastname: Tanigaki-Obana
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Tazawa
authors.forename: M
authors.initials: M
authors.lastname: Ito
mesh_terms: D046508:Culture Techniques; D006197:Hair / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis*
chemical_list: D007633:Keratins