title: Searching for synaptonemal complex proteins and their genes.
abstract: As an alternative to the production and use of monoclonal antisynaptonemal complex (SC) antibodies to isolate SC genes, we have explored the use of polyclonal anti-SC antibodies to identify SC genes from a cDNA expression library. The method proved relatively simple, reliable, and fast and has yielded two SC genes. A homologue of one of these genes from a different species has previously been isolated in another laboratory.
authors.affiliation: Department of Biology, York University, Downsview, Ontario, Canada.
authors.forename: P B
authors.initials: PB
authors.lastname: Moens
authors.forename: B
authors.initials: B
authors.lastname: Spyropoulos
authors.forename: M
authors.initials: M
authors.lastname: Dobson
authors.forename: A
authors.initials: A
authors.lastname: Karaiskakis
authors.forename: R E
authors.initials: RE
authors.lastname: Pearlman
mesh_terms: D000818:Animals; D000906:Antibodies; D006224:Cricetinae; D004247:DNA; D005796:Genes; D011506:Proteins / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D013573:Synaptonemal Complex / Q000235:genetics*
chemical_list: D000906:Antibodies; D011506:Proteins; D004247:DNA