title: Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.
abstract: A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited number of sites in the membrane. A qualitative study shows that an increase in the labelling leads to an evolution of the probe fluorescence properties; the existence of different types of sites is involved in the interpretation but the system is too complex to allow it to be represented by a simple model. As a consequence of this study, care has to be taken in labelling biological material so as to avoid excessive probe incorporation.
authors.affiliation: LA 328 du CNRS, ENSIC, Nancy, France.
authors.forename: C
authors.initials: C
authors.lastname: Maire
authors.forename: M
authors.initials: M
authors.lastname: Bouchy
authors.forename: M
authors.initials: M
authors.lastname: Donner
authors.forename: J C
authors.initials: JC
authors.lastname: André
mesh_terms: D004161:Diphenylhexatriene / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D006801:Humans; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological
chemical_list: D005456:Fluorescent Dyes; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene