Effect of a tyrosine kinase inhibitor, genistein, on the actions of prolactin in cultured mouse mammary tissues.
Genistein, an inhibitor of tyrosine kinase, was used to determine the possible role of tyrosine kinase in the prolactin (PRL) stimulation of milk product formation and ornithine decarboxylase (ODC) activation in cultured mouse mammary gland tissue. Genistein (10-200 microM) inhibited in a dose-response fashion the PRL stimulation of casein, lipid and lactose synthesis as well as ODC activation. Genistein, however, did not inhibit the phospholipase C, phorbol myristate acetate or cAMP effects on ODC activation. These results suggest the possible involvement of tyrosine kinase in the mechanism by which PRL expresses its effects in mammary gland tissues.
Fan
G
G
Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201.
Rillema
J A
JA
D000818:Animals; D002364:Caseins / Q000096:biosynthesis; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation; D005260:Female; D019833:Genistein; D007529:Isoflavones / Q000494:pharmacology*; D007785:Lactose / Q000096:biosynthesis; D008055:Lipids / Q000096:biosynthesis; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011247:Pregnancy; D011388:Prolactin / Q000187:drug effects / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism
D002364:Caseins; D007529:Isoflavones; D008055:Lipids; D011388:Prolactin; D019833:Genistein; D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; D010738:Type C Phospholipases; D009955:Ornithine Decarboxylase; D007785:Lactose; D013755:Tetradecanoylphorbol Acetate