The first 124 nucleotides of the E7 coding sequences of HPV16 can render the HPV11 genome transformation competent.
The human papillomavirus (HPV) type 16 is associated with the majority of cervical tumors and is capable of oncogenically transforming cells in culture. HPV type 11 is rarely associated with malignant lesions and does not transform cells in vitro. While the E7 of HPV16 is necessary and sufficient for transformation of rodent cells, the E7 gene of HPV11 is not transforming. In the present report we demonstrate that the HPV11 genome, with the first 124 nucleotides of the HPV16 E7 open reading frame fused to the last 198 nucleotides of the HPV11 E7 open reading frame, becomes transformation competent.
Pater
M M
MM
Basic Medical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.
Nakshatri
H
H
Kisaka
C
C
Pater
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006224:Cricetinae; D004252:DNA Mutational Analysis; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics / Q000472:pathogenicity*; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D013329:Structure-Activity Relationship
D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; C059731:oncogene protein E7, Human papillomavirus type 16