Genomic organization and analysis of the 5' end of the porcine ryanodine receptor gene (ryr1).
In this study we describe the isolation of genomic clones of the 5' region of the porcine ryanodine receptor gene, a candidate for malignant hyperthermia in pigs and humans. The recombinants were isolated from a porcine liver, genomic DNA library in phage EMBL3A after screening with PCR amplified DNA fragments. The exon/intron structure of the ryanodine receptor gene was determined by DNA sequencing. Based on the sequence data it was possible to develop a simple test for the detection of malignant hyperthermia susceptible and normal pigs.
Brenig
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B
Lehrstuhl für Molekulare Tierzucht, Veterinärmedizinische Fakultät Ludwig-Maximilians-Universität München, Germany.
Brem
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G
D000818:Animals; D001483:Base Sequence; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism; D004247:DNA; D006801:Humans; D008305:Malignant Hyperthermia / Q000235:genetics / Q000662:veterinary; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011950:Receptors, Cholinergic / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D019837:Ryanodine Receptor Calcium Release Channel; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000235:genetics
D015220:Calcium Channels; D011950:Receptors, Cholinergic; D019837:Ryanodine Receptor Calcium Release Channel; D004247:DNA