Endothelin stimulates both cAMP formation and phosphatidylinositol hydrolysis in cultured embryonic bovine tracheal cells.
Embryonic bovine tracheal (EBTr) cells were found to possess receptors for endothelin (ET) of ET-1-selective (ETA) subtype with a Kd for ET-1 of 114 pM and a Bmax of 12.9 fmol/10(5) cells. Stimulation of EBTr cells with 100 pM to 100 nM ET-1 increased the contents of both inositol phosphates and cAMP in a concentration-dependent manner, indicating that the receptors are coupled to both phosphatidylinositol hydrolysis and cAMP formation in EBTr cells.
Oda
K
K
International Research Laboratories, Ciba-Geigy Ltd., Takarazuka, Japan.
Fujitani
Y
Y
Watakabe
T
T
Inui
T
T
Okada
T
T
Urade
Y
Y
Okuda-Ashitaka
E
E
Ito
S
S
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis*; D016232:Endothelins / Q000494:pharmacology*; D006868:Hydrolysis; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D011956:Receptors, Cell Surface / Q000378:metabolism; D017466:Receptors, Endothelin; D014132:Trachea / Q000166:cytology / Q000378:metabolism*
D016232:Endothelins; D010716:Phosphatidylinositols; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D000242:Cyclic AMP