[Effect of the tumor promoter phorbol myristate acetate (PMA) on cell-mediated immunity. IV. PMA-induced modulation of mouse dendritic cell function].
In the present study we investigated the in vitro effects of the tumor promoter phorbol myristate acetate (PMA) on the accessory function of mouse spleen dendritic cells (DC) in mitogenesis. The effects of the PMA, a protein kinase C (PKC) activator, depended on dose. If pretreated with < 50 ng/ml of PMA for 3 h, DC activity was enhanced by about two-fold; whereas > or = 200 ng/ml of PMA decreased DC activity with an inhibition rate about 50% (in early response DC activity showed a moderate increase). Our results indicate that the function of DC, a potent accessory population, can be further enhanced by a low dose of PMA. This is circumstantial evidence that DC activity can be up-regulatal via PKC activation. That a high dose of PMA inhibits DC activity might be a mechanism by which PMA promotes carcinogenesis.
Liu
C
C
Cancer Institute, Beijing.
Zhang
Y
Y
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007111:Immunity, Cellular / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D013755:Tetradecanoylphorbol Acetate