A Hox 3.3-lacZ transgene expressed in developing limbs.
We describe transgenic mouse lines that express lacZ under the control of the Hox 3.3 Promoter II. The correct anterior boundary can be fixed by 3.6 kb of promoter DNA (plus 1.6 kb of 5' transcribed sequences), both in tissues of ectodermal and mesodermal origin. The posterior border, however, is not respected, and lacZ expression continues into the tail region. One line has particularly strong graded expression in the anterior proximal limb bud. Other lines, containing a shorter promoter fragment (0.6 kb), have ectopic expression in the head region, including one line that has expression in the anterior half of the retina. Such mouse lines make it possible to molecularly distinguish cells in regions of the embryo that look otherwise identical and may be useful in studying the establishment of molecular differences in the mouse embryo.
Jegalian
B G
BG
Department of Biological Chemistry, University of California, Los Angeles 90024.
Miller
R W
RW
Wright
C V
CV
Blum
M
M
De Robertis
E M
EM
D000818:Animals; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000502:physiology*; D005121:Extremities / Q000196:embryology*; D005786:Gene Expression Regulation / Q000502:physiology*; D005796:Genes / Q000502:physiology; D018398:Homeodomain Proteins; D007763:Lac Operon / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic / Q000502:physiology; D012160:Retina / Q000378:metabolism
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C080041:Hoxc6 protein, mouse