[Use of computer-assisted image analysis for the detection of immunohistochemical receptor status in breast cancer. Immunohistochemical densitometry receptor analysis--IRDA].
A method for the evaluation of the immunohistochemical staining score of steroid receptors in breast cancer using computer-based image analysis is presented. The optical density of tumour cell nuclei was measured using kryostat sections and, using a control section as reference, an objective threshold for specific staining was defined. In intensively stained sections, a good correlation with visual scoring was achieved, but in 9 out of 16 sections, which were regarded as receptor-negative by visual scoring, a specific receptor-positive staining was demonstrated by image analysis. Next to the percentage of positive cells (PP) and the mean staining intensity (SI), a histogram of the receptor concentration in positive nuclei was calculated. The method allows, besides an objective evaluation and documentation, an exact definition of a threshold for specific receptor measurement and furthermore shows the pattern of receptor concentration in the tumour cell nuclei. Hence, the method could contribute towards a more widespread use of immunohistochemical receptor analysis.
Mink
D
D
Universitäts-Frauenklinik und Poliklinik mit Hebammenlehranstalt, Homburg/Saar.
Bonkhoff
H
H
Herth
G
G
Schmidt
W
W
D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D007150:Immunohistochemistry / Q000295:instrumentation*; D009376:Neoplasms, Hormone-Dependent / Q000473:pathology*; D011379:Prognosis; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D012984:Software
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone
Einsatz der computergestützten Bildanalyse zur Erfassung des immunhistochemischen Rezeptorstatus beim Mammakarzinom. Immunhistochemische densitometrische Rezeptoranalyse--IDRA.