title: An immunoperoxidase method applied to the diagnosis of equine herpesvirus abortion, using conventional and rapid microwave techniques.
abstract: An indirect immunoperoxidase (IMP) technique was applied to cryostat and paraffin sections of liver from ten aborted equine foetuses. Equid herpesvirus type 1 (EHV-1) had been isolated from seven of them and EHV-4 from one: the remaining two were virologically negative and were not used as controls. In the eight virus-infected cases the immunostaining revealed foci of cells exhibiting a distinct brown cytoplasmic and inclusion body pigmentation. No specific signal was present in the non-infected controls. The method also was adapted for incubation in a microwave oven, which allowed the total length of the procedure to be reduced to a minimum of 35 mins for cryostat sections, without significant loss of signal.
authors.affiliation: Department of Infectious Diseases, Animal Health Trust, Newmarket, Suffolk, UK.
authors.forename: K E
authors.initials: KE
authors.lastname: Whitwell
authors.forename: S M
authors.initials: SM
authors.lastname: Gower
authors.forename: K C
authors.initials: KC
authors.lastname: Smith
mesh_terms: D000034:Abortion, Veterinary / Q000175:diagnosis*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006564:Herpesviridae / Q000276:immunology / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000276:immunology / Q000302:isolation & purification*; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D007124:Immunoenzyme Techniques; D008099:Liver / Q000196:embryology / Q000382:microbiology / Q000473:pathology; D008872:Microwaves; D009336:Necrosis; D016612:Paraffin Embedding; D011247:Pregnancy
chemical_list: D000956:Antigens, Viral