Sickle cells modified by an osmotic pulse in the presence of inositol hexaphosphate have decreased intracellular hemoglobin concentration and decreased in vitro sickling without prolonged in vivo survival.
An osmotic pulse was used to modify red blood cells (RBC) from two patients with sickle cell disease, resulting in an increased volume and decreased hemoglobin content. This treatment yielded cells which were divided into two populations, one in which RBC had markedly decreased hemoglobin concentration and another in which the cells appeared to be unmodified. Morphological sickling at low oxygen partial pressure was markedly decreased. However, there was no evidence for increased RBC lifespan when the 51Cr-labeled, modified cells were reinfused.
Franco
R
R
University of Cincinnati, College of Medicine, OH.
Barker-Gear
R
R
Silberstein
E
E
Mayfield
G
G
Weiner
M
M
Palascak
J
J
Green
R
R
D000755:Anemia, Sickle Cell / Q000097:blood / Q000473:pathology*; D004905:Erythrocyte Aging / Q000187:drug effects*; D004913:Erythrocytes, Abnormal / Q000187:drug effects* / Q000378:metabolism; D006451:Hemoglobin, Sickle / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009997:Osmotic Pressure; D010833:Phytic Acid / Q000494:pharmacology*; D013997:Time Factors
D006451:Hemoglobin, Sickle; D010833:Phytic Acid