[Colony hybridization with digoxigenin labelled DNA probe].
A simple, rapid method for colony hybridization has been developed. The DNA probes were labelled by digoxigenin. Hybridization signal was detected by enzyme-linked immunoassay reaction. The results showed that this method is sensitive and reproducible, and it can be used for colony hybridization instead of isotopic methods.
Jiang
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Zhang
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D003001:Cloning, Molecular; D015342:DNA Probes; D004274:DNA, Recombinant; D004076:Digoxigenin; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D017403:In Situ Hybridization; D010957:Plasmids / Q000235:genetics
D015342:DNA Probes; D004274:DNA, Recombinant; D004076:Digoxigenin