Surface charge and hydrophobicity of wild and mutant Crithidia fasciculata.
Surface charge of wild-type Crithidia fasciculata and three drug-resistant mutants (TR3, TFRR1, and FUR11) was studied by direct zeta-potential determination and ultrastructural cytochemistry. Surface tension was also investigated by measurements of the advancing contact angle formed by the protozoa monolayers with drops of liquids of different polarities. The individual zeta potential varies markedly among the C. fasciculata cells. The wild and FUR11 mutant strains displayed lower negative surface charge (-12.5 and -9.5 mV, respectively) as compared with the TR3 (-14.8 mV) and TFRR1 (-14.7 mV) mutant strains. Binding of cationized ferritin (CF) was observed at the cell surface of wild and mutant strains of C. fasciculata. Neuraminidase treatment reduced the negative surface charge in the TFRR1 and TR3 mutants in about 37 and 29%, respectively, whereas no significant change was observed with the wild and FUR11 mutant strains. These findings suggest that sialic acid residues are the major anionogenic groups on the surface of C. fasciculata. The density of sialic acid residues per cell in wild and mutant strains of C. fasciculata falls in a range of 1.4 x 10(4) to 3.6 x 10(4). Marked differences of hydrophobicity were also observed. For example, the TFRR1, FUR11, and TR3 drug-resistant mutant strains showed higher contact angle values (55.4, 54.2, and 49.3, respectively) than the wild-type (35.6), as assessed by alpha-bromonaphtalene.
Matta
M A
MA
Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brazil.
Alviano
C S
CS
Angluster
J
J
De Souza
W
W
Silva-Filho
F C
FC
Esteves
M J
MJ
D000818:Animals; D000838:Anions; D003421:Crithidia / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics; D006651:Histocytochemistry; D008564:Membrane Potentials / Q000502:physiology; D008769:Methylnitronitrosoguanidine; D009154:Mutation; D012016:Reference Values; D012995:Solubility; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
D000838:Anions; D014867:Water; D008769:Methylnitronitrosoguanidine