Analysis of ecdysteroids in the trematodes, Schistosoma mansoni and Fasciola hepatica.
Adult Schistosoma mansoni from experimentally infected mice and Fasciola hepatica recovered from ovine livers post mortem were analyzed for free and conjugated ecdysteroids by radioimmunoassay, high-performance liquid chromatography monitoring fractions by radioimmunoassay, and by capillary gas chromatography/mass spectrometry (selected ion monitoring). Both species contained ecdysone and 20-hydroxyecdysone as free ecdysteroids and as polar conjugates. F. hepatica also contained a polar conjugate of 2-deoxyecdysone. Evidence of apolar ecdysteroid conjugates was only obtained for F. hepatica. The free ecdysteroid-containing fraction of S. mansoni contained small amounts of unidentified immunoreactive material.
Foster
J M
JM
Department of Biochemistry, University of Liverpool, U.K.
Mercer
J G
JG
Rees
H H
HH
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004440:Ecdysone / Q000032:analysis; D026461:Ecdysteroids; D004441:Ecdysterone / Q000032:analysis; D005210:Fasciola hepatica / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007447:Invertebrate Hormones / Q000032:analysis*; D011863:Radioimmunoassay; D012550:Schistosoma mansoni / Q000737:chemistry*; D013256:Steroids / Q000032:analysis*
D026461:Ecdysteroids; D007447:Invertebrate Hormones; D013256:Steroids; D004440:Ecdysone; D004441:Ecdysterone