High-throughput DNA preparation system.
A system demonstrating the feasibility of high-throughput, centrifugation-based DNA separations and purifications has been constructed and tested. Samples are currently processed at a rate of 96 in approximately 2-3 h. The device implements an automation-optimized alkaline lysis protocol for the rapid extraction of plasmid or cosmid DNA from 1-ml bacteria cultures. The conditions for optimal culturing in deep-well (96 x 1 ml) microwell plates have been developed, and all sample manipulations are done within these plates. The use of microwell plates was essential to obtain high throughput and make manipulations following the DNA preparation (prep) easier because they can then be manipulated using a variety of commercially available robots. The entire prep system is constructed above a Beckman GPR centrifuge and operated under Macintosh IIcx control. This device has systems for fluid handling, microwell-plate manipulations, and centrifuge rotor alignment.
Garner
H R
HR
Institute for Development of Advanced Technology, General Atomics, San Diego, CA 92186.
Armstrong
B
B
Kramarsky
D A
DA
D001331:Automation; D002498:Centrifugation; D003360:Cosmids / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004926:Escherichia coli; D010957:Plasmids / Q000302:isolation & purification*; D013053:Spectrophotometry
D004269:DNA, Bacterial