[The effects of different lipoproteins on cholesterol metabolism in smooth muscle cells of rabbit aorta].
In the present experiment, M-SMC were cultured from rabbit aorta by an explant method and I-SMC cultured by the explant method from cannulated aortic intima of rabbits, and the effects of LDL, A-LDL, OX-LDL and HDL on the cholesterol metabolism in both types of cells were investigated by using 14C oleic acid as the source of cholesterol re-esteri fication in cells. The results showed that LDL enhanced cholesterol re-esterification in both types of cells and HDL had an opposite effect, A-LDL could increase CE synthesis only in I-SMC, while OX-LDL showed a complex effect on the level of CE in different cells by different concentration. The present experiment has studied the relationship between lipoprotein and cholesterol metabolism in SMC at the level of cell metabolism, and suggests that lipoproteins play a key role in AS.
Xu
Q P
QP
Department of Cardiology, First Affiliated Hospital of Zhejiang Medical University, Hangzhou.
Huang
Y W
YW
D000818:Animals; D001011:Aorta / Q000187:drug effects; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D010084:Oxidation-Reduction; D011817:Rabbits; D017539:Tunica Intima / Q000187:drug effects; D017540:Tunica Media / Q000187:drug effects
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; C032629:acetyl-LDL; D002784:Cholesterol