Passive avoidance learning in the young chick results in time- and locus-specific elevations of alpha-tubulin immunoreactivity.
A monoclonal antibody was used to examine changes in immunoreactivity of the cytoskeletal protein, alpha-tubulin, following passive avoidance learning in day-old chicks. Postmitochondrial fractions (16,000 g supernatants) were prepared from specific forebrain loci taken at several time points after training and assayed with the anti-alpha-tubulin antibody, YL1/2. Of the regions examined, elevations in the titre of YL1/2 were found in the left intermediate hyperstriatum ventrale 1 h, 6 h and 24 h following training, in the left lobus parolfactorius 1 h following training and in the right lobus parolfactorius 6 h and 24 h following training. No training-related changes were detected in a third forebrain region, the paleostriatum augmentatum. These results regarding the cellular dynamics of memory formation in the chick confirm and expand on earlier findings from our laboratory.
Scholey
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AB
Department of Biology, Open University, Milton Keynes, U.K.
Bullock
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S
Rose
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SP
D000818:Animals; D000911:Antibodies, Monoclonal; D001362:Avoidance Learning / Q000502:physiology*; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D009928:Organ Specificity; D016548:Prosencephalon / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D014404:Tubulin / Q000032:analysis / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D014404:Tubulin