title: [The effect of natural and synthetic antioxidant polyhydroxynaphthoquinones on cholesterol metabolism in cultured rabbit hepatocytes].
abstract: Primary cultures of rabbit hepatocytes were used to examine the effect of natural and synthetic antioxidants--polyhydroxynaphthoquinones (PHNQ) and alpha-tocopherol on cholesterol and bile acid synthesis. Histochrome, one of the PHNQ, slightly decreased cholesterol synthesis at concentrations 10-100 microM, whereas alpha-tocopherol stimulated cholesterol synthesis. After administration of histochrome or alpha-tocopherol into culture medium a significant stimulation of bile acid synthesis in dose-dependent manner was observed. The increase of bile acid secretion by histochrome in the presence of physiological concentration of HDL2 was found as well. Since histochrome in contrast to alpha-tocopherol enhanced accumulation of [14C] cholesterol of HDL2 in the hepatocytes, it was concluded that histochrome stimulated bile acid synthesis as a result of increased input of HDL2 cholesterol into hepatocytes. These data suggest that histochrome may exhibit a hypocholesterolemic effect by stimulation of bile acid synthesis and inhibition of cholesterol synthesis.
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Lakeev
authors.forename: V A
authors.initials: VA
authors.lastname: Kosykh
authors.forename: E I
authors.initials: EI
authors.lastname: Kosenkov
authors.forename: V L
authors.initials: VL
authors.lastname: Novikov
authors.forename: A V
authors.initials: AV
authors.lastname: Lebedev
authors.forename: V S
authors.initials: VS
authors.lastname: Repin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001647:Bile Acids and Salts / Q000096:biosynthesis; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D002784:Cholesterol / Q000096:biosynthesis; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009285:Naphthoquinones / Q000494:pharmacology*; D011817:Rabbits; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001647:Bile Acids and Salts; D009285:Naphthoquinones; D014810:Vitamin E; D002784:Cholesterol
vernacular_title: Vliianie prirodnykh i sinteticheskikh antioksidantov--poligidroksinaftakhinonov na metabolizm kholesterina v kul'tiviruemykh gepatotsitakh krolika.