title: 1H NMR assignment and secondary structure of the cell adhesion type III module of fibronectin.
abstract: The secondary structure of the tenth type III module from human fibronectin has been determined using NMR. This type of module appears many times in a wide variety of proteins. The type III module described here contains an Arg-Gly-Asp sequence known to be involved in cell-cell adhesion. The module was expressed in yeast and characterized by amino acid sequencing and mass spectrometry. 2D and 3D NMR spectroscopy of 15N-labeled protein was used to perform sequence-specific assignment of the spectrum. The secondary structure was defined by patterns of nuclear Overhauser effects, 3JNH-alpha CH spin-spin coupling constants, and amide proton solvent exchange rates. The molecule consists of seven beta-strands in two antiparallel beta-sheets with an immunoglobulin-like fold similar to that predicted for homologous modules in the cytokine receptor super family [Bazan, J. F. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 6934-6938]. The Arg-Gly-Asp sequence is located on a loop between the beta-strands F and G.
authors.affiliation: Department of Biochemistry, University of Oxford, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Baron
authors.forename: A L
authors.initials: AL
authors.lastname: Main
authors.forename: P C
authors.initials: PC
authors.lastname: Driscoll
authors.forename: H J
authors.initials: HJ
authors.lastname: Mardon
authors.forename: J
authors.initials: J
authors.lastname: Boyd
authors.forename: I D
authors.initials: ID
authors.lastname: Campbell
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002448:Cell Adhesion; D004247:DNA / Q000235:genetics; D005353:Fibronectins / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011522:Protons; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D005353:Fibronectins; D011522:Protons; D004247:DNA