[The facilitation of defensive reactions during food consumption in the snail helix: the participation of glucose and gastrin/cholecystokinin-like peptide].
Molecular and cellular mechanisms of the interrelations between the feeding and defense behaviour were studied in a snail Helix lucorum. The dynamics of defense reactions was investigated in snails with different levels of feeding motivation. Defense reactions were suppressed in hungry snails, while 15-20 min after the beginning of food intake they were facilitated. The facilitation depended on a duration of starvation. Injection of 0.5 ml of 5 mM glucose solution (up to the glucose level in the haemolymph of a food satiated snail, 1.6-2.0 mM) or injections of 20-30 ng of synthetic analogues of the gastrointestinal peptides (pentagastrin of octapeptide cholecystokinin, CCK-8) facilitated the defense reaction in a hungry snail. Parameters of the facilitation were similar to those in the period of food intake. Activity of the command neurons of defense behaviour (L-PPL1) after the carrot juice application to the lip of a semi-intact preparation from a hungry snail was glucose-dependent. Similar glucose-dependent changes of L-PPL1 activity were found after CCK-8, but not FMRFamide application during the perfusion with 0.5 mM glucose. L-PPL1, but not L-PPa2-3 neurons were most sensitive to glucose and CCK-8 level changes in the Ringer solution. Adaptive significance of the behavioural phenomena as well as glucose and gastrin/CCK-like peptide participation in these processes are discussed.
Shevëlkin
A V
AV
D000818:Animals; D004924:Escape Reaction / Q000187:drug effects / Q000502:physiology*; D019835:FMRFamide; D005215:Fasting / Q000502:physiology; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology*; D005508:Food Deprivation / Q000502:physiology; D005947:Glucose / Q000494:pharmacology / Q000502:physiology*; D006372:Helix, Snails / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009479:Neuropeptides / Q000494:pharmacology; D010418:Pentagastrin / Q000494:pharmacology; D012844:Sincalide / Q000494:pharmacology / Q000502:physiology*; D013373:Substance P / Q000494:pharmacology; D013997:Time Factors
D009479:Neuropeptides; D013373:Substance P; D019835:FMRFamide; D010418:Pentagastrin; D005947:Glucose; D012844:Sincalide
Oblegchenie oboronitel'nykh reaktsiĭ v period potrebleniia pishchi u vinogradnykh ulitok: uchastie gliukozy i gastrin/kholetsistokininpodobnogo peptida.