Beta 2-adrenergic receptor mRNA is overexpressed in neoplastic human thyroid tissues.
The expression of beta 2-adrenergic receptor (AR) mRNA was investigated in normal and neoplastic human thyroid tissues. A combination of techniques for reverse transcribing mRNA into cDNA and the incorporation of 32P-gamma ATP into the polymerase chain reaction (PCR)-generated fragments allowed us to detect beta 2-AR mRNA in surgically excised thyroid specimens. The levels of beta 2-AR cDNA generated by PCR in thyroid adenomas and cancers were 3.3 and 6.9 times, respectively, as high as that of normal thyroid tissues. These findings suggest that the level of beta 2-AR mRNA is correlated with the extent of differentiation in neoplastic tissues. The present study provides new insights into the relationships between the AR-adenylate cyclase system and the regulation of the growth and differentiation in neoplastic human thyroid tissues.
Ling
L
L
Third Department of Internal Medicine, University of Yamanashi Medical School, Japan.
Haraguchi
K
K
Ohta
K
K
Endo
T
T
Onaya
T
T
D001483:Base Sequence; D004247:DNA / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D011943:Receptors, Adrenergic, beta / Q000235:genetics*; D011989:Receptors, Thyrotropin / Q000235:genetics; D013964:Thyroid Neoplasms / Q000378:metabolism*
D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D011989:Receptors, Thyrotropin; D004247:DNA