Quantification of the c-myc gene in gastric carcinomas by the triplex polymerase chain reaction and high performance liquid chromatography.
The triplex polymerase chain reaction (PCR) and high performance liquid chromatography (HPLC) techniques were used to examine the state of amplification of the c-myc gene in gastric carcinomas. Sequences from the c-myc gene and from the two control genes were coamplified by PCR. The coamplified PCR products were separated and quantified by HPLC and the copy numbers of the c-myc gene were calculated by comparing the peak areas generated by PCR products. Increased copy numbers of the c-myc gene were found in 2 of 5 patients.
Yoshimura
S
S
Division of Molecular Life Science (Cell Biology), Tokai University School of Medicine, Kanagawa, Japan.
Shishikura
A
A
Koido
S
S
Ushigome
S
S
Suemizu
H
H
Taniguchi
Y
Y
Moriuchi
T
T
D002851:Chromatography, High Pressure Liquid; D004273:DNA, Neoplasm / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D016259:Genes, myc; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013274:Stomach Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm