title: On the anomalous behaviour on gel-filtration and SDS-electrophoresis of prothymosin-alpha.
abstract: The regulator of T-cell proliferation prothymosin alpha, is a protein with a relative molecular mass of 12 KDa as calculated from its amino acid sequence. This immunoregulator exhibits anomalous behaviour on gel-filtration and SDS-PAGE electrophoresis appearing as oligomers which are 5 or 2 fold larger than the corresponding polypeptide. These results suggest that a dimeric form of prothymosin alpha is stable to dissociation by SDS and reduction by beta-mercapto ethanol.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Santiago, Santiago de Compostela, Spain.
authors.forename: O J
authors.initials: OJ
authors.lastname: Cordero
authors.forename: C S
authors.initials: CS
authors.lastname: Sarandeses
authors.forename: J L
authors.initials: JL
authors.lastname: Lopez
authors.forename: M
authors.initials: M
authors.lastname: Nogueira
mesh_terms: D000328:Adult; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D010835:Phytohemagglutinins; D011498:Protein Precursors / Q000737:chemistry* / Q000494:pharmacology; D013947:Thymosin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D010835:Phytohemagglutinins; D011498:Protein Precursors; C040782:prothymosin alpha; D013947:Thymosin