title: Broad-complex function during oogenesis in Drosophila melanogaster.
abstract: The Broad-Complex (BR-C) appears to encode factors that mediate ecdysone effects during the larva-adult transition. The main goal of this study was to gain insight into what roles the BR-C might play during oogenesis. The main findings are as follows. First, as determined by heteroallele studies and clonal analysis, de12 is a somatic line mutation that appears to fall into the broad domain of the BR-C. Second, the de12 mutation is associated with the insertion of the gypsy transposon at position 169.5 (Chao and Guild, Embo J, 1986, 5:143-150) in the BR-C domain. In its new context this gypsy element exhibits ovarian-specific activation. Both this gypsy activation and the de12 phenotype are partially suppressible by su(f) and su(Hw). Third, we have identified a set of transcripts that cross-hybridize with BR-C sequence spanning the gypsy insertion site (166-179). There are significant differences in these cross-hybridizing species, both in size and relative abundance, between de12 and its parent strain. Finally we have determined that in de12 there is a premature arrest of chorion gene amplification in the late stages of oogenesis.
authors.affiliation: Department of Biological Sciences, Southern Methodist University, Dallas, Texas 75275.
authors.forename: R Y
authors.initials: RY
authors.lastname: Huang
authors.forename: W C
authors.initials: WC
authors.lastname: Orr
mesh_terms: D000818:Animals; D001483:Base Sequence; D002823:Chorion / Q000502:physiology; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology; D004440:Ecdysone / Q000502:physiology; D005260:Female; D005816:Genetic Complementation Test; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D009866:Oogenesis / Q000235:genetics; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D004440:Ecdysone