Mobilization of an intracellular glycoprotein (Mac-1) on monocytes and granulocytes during hemodialysis.
We studied the upregulation of the intracellular glycoprotein Mac-1 (CD11b/CD18, CR3) on monocytes and granulocytes during 36 bicarbonate hemodialyses in 12 patients who were randomly treated with Cuprophan (Cu), Hemophan (He) or Polysulfone (PS; low-flux) membranes. The degree of mobilization of this adhesion protein was related to changes in granulocyte and monocyte count, generation of C3a and production of interleukin-1 beta in plasma. Mac-1 expression on granulocytes was significantly higher after 5 and 15 min of Cu hemodialysis as compared to He or PS dialyses (p < 0.001) and correlated to changes in granulocyte count at 15 min (r = 0.62 and r = 0.76, p < 0.001). No differences in early Mac-1 mobilization on circulating monocytes was observed despite a decrease in cell count. Mac-1 expression on monocytes and granulocytes in the venous blood line at 180 min of treatment was significantly higher during Cu dialysis as compared to He and PS dialyses (p < 0.02 and p < 0.001, respectively). Early generation of C3a was higher in patients on Cu dialysis than in He or PS dialysis (p < 0.001) and correlated both to granulocytopenia (r = 0.45, p < 0.01) and to the subsequent increase in Mac-1 expression on granulocytes (r = 0.63, p < 0.001). An early increase in Mac-1 expression on monocytes was accompanied by an increase in plasma interleukin-1 beta later during dialysis (p < 0.05). Studies of Mac-1 expression during hemodialysis increased the sensitivity of biocompatibility measurements and correlated better than complement generation to changes in granulocyte count as it mediates adhesion to endothelial cells.
Thylén
P
P
Department of Medicine, Karolinska Hospital, Stockholm, Sweden.
Lundahl
J
J
Fernvik
E
E
Hed
J
J
Svenson
S B
SB
Jacobson
S H
SH
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002482:Cellulose / Q000031:analogs & derivatives; D003167:Complement Activation; D015926:Complement C3a / Q000032:analysis; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000097:blood; D007958:Leukocyte Count; D016177:Macrophage-1 Antigen / Q000097:blood*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D011108:Polymers; D006435:Renal Dialysis / Q000295:instrumentation; D013450:Sulfones; D015854:Up-Regulation
D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules; D007375:Interleukin-1; D016177:Macrophage-1 Antigen; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C054645:Hemophan; C017662:polysulfone P 1700; D015926:Complement C3a; D002482:Cellulose; C009865:cuprammonium cellulose