[Development of a system for cloning a DNA fragment, containing the determinant of resistance to actinomycin for Streptomyces chrysomallus No.2--a producer of actinomycin C].
To study the modes of actinomycin biosynthesis and the mechanism responsible for resistance to the antibiotic producing S. chrysomallus No. 2, the authors undertook an examination and studies into the cloning system for gene(s) of resistance to actinomycin from a S. chrysomallus No. 2 actinomycin C producer and the cloning of a S. chrysomallus No. DNA fragment to the actinomycin-sensitive Streptomyces Sp. 26-115 H-I on the vector plasmid pIJ702. The cloning gave rise to actinomycin-resistant strains. The character of actinomycin resistance is inheritable in a steady fashion.
Novozhilova
T Iu
TIu
Alekhova
T A
TA
D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D003609:Dactinomycin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D004352:Drug Resistance, Microbial / Q000235:genetics; D005822:Genetic Vectors; D013302:Streptomyces / Q000378:metabolism*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; C030013:cactinomycin; D003609:Dactinomycin
Razrabotka sistemy klonirovaniia fragmenta DNK, soderzhashchego determinat ustoĭchivosti k aktinomitsinu Streptomyces chrysomallus No. 2--produtsenta aktinomitsina C.