Concentration-dependent modulation of basic fibroblast growth factor action on multiplication and locomotion of human teratocarcinoma cells.
A human teratoma cell line (Tera 2) was grown in serum-free medium, and the population multiplication was stimulated by the addition of 1-10 ng basic fibroblast growth factor (bFGF)/ml. The bFGF-effect was abrogated by the addition of protamine sulphate. When high concentrations of bFGF were added, a preferential effect on cell locomotion was observed. 100 ng bFGF/ml stimulated cell movement but only exerted a marginal effect on cell multiplication. These observed exogenous requirements for multiplication and locomotion were complemented by the expression of bFGF receptors. Scatchard analysis of binding data suggests the existence of a high-affinity and a low-affinity class of receptors.
Schofield
P N
PN
Department of Anatomy, University of Cambridge, UK.
Granerus
M
M
Lee
A
A
Ektröm
T J
TJ
Engström
W
W
D002455:Cell Division / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D016222:Fibroblast Growth Factor 2 / Q000502:physiology*; D006801:Humans; D011956:Receptors, Cell Surface / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor; D013724:Teratoma; D014407:Tumor Cells, Cultured
D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2