Inhibitory effect of oryzacystatins and a truncation mutant on the replication of poliovirus in infected Vero cells.
Poliovirus, a picornavirus family member, requires the processing of its poly-protein by its own cysteine proteinase for replication. Oryzacystatin-I and oryzacystatin-II, proteinaceous cysteine proteinase inhibitors (cystatins) of rice seed origin, were found to inhibit the replication of poliovirus effectively in infected Vero cells in vitro. Truncated oryzacystatin-I, which lacks the NH2-terminal 25 amino acid residues of the intact protein, is an even more effective inhibitor, eliciting its effect at concentrations of less than 0.25 nmol/ml. The low molecular weight cysteine proteinase inhibitors, E-64, E-64C and loxistatin, showed no anti-viral effect at any concentration investigated.
Kondo
H
H
Department of Agricultural Chemistry, University of Tokyo, Japan.
Ijiri
S
S
Abe
K
K
Maeda
H
H
Arai
S
S
D000818:Animals; D015891:Cystatins / Q000235:genetics / Q000494:pharmacology*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D009154:Mutation; D017955:Poliovirus / Q000502:physiology*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*
D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors