title: Expression of feline immunodeficiency virus gag gene in Escherichia coli.
abstract: The gag gene of a Japanese feline immunodeficiency virus (FIV) isolate, designated as FIV TM 2, was expressed in Escherichia coli as a fusion protein with TrpE. Using this expressed protein, an enzyme-linked immunosorbent assay was developed for detection of antibodies to FIV gag protein in feline sera. With serum samples from a cat experimentally infected with FIV, it was demonstrated that the period of seroconversion detected by this method corresponded to that by Western blotting.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Furuya
authors.forename: A
authors.initials: A
authors.lastname: Hasegawa
authors.forename: M
authors.initials: M
authors.lastname: Saitoh
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: Y
authors.initials: Y
authors.lastname: Tohya
authors.forename: M
authors.initials: M
authors.lastname: Hayami
authors.forename: E
authors.initials: E
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Miki
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D015683:Gene Products, gag / Q000235:genetics*; D016089:Immunodeficiency Virus, Feline / Q000235:genetics*; D010957:Plasmids; D011993:Recombinant Fusion Proteins
chemical_list: D015683:Gene Products, gag; D011993:Recombinant Fusion Proteins