Effect of LH and prolactin on steroid secretion by perifused luteal tissue from pregnant gilts with induced hypoprolactinemia or after passive immunoneutralization of LH.
The study was performed using luteal tissue obtained from 24 pregnant gilts. Group I was treated with bromocriptine (BR) from 37th to 42nd day of gestation. Group II was treated with homologous anti-pLH serum from 37th to 42nd day of pregnancy. Group III was given BR from 67th to 72nd day of gestation. Group IV received anti-pLH serum from 67th to 72nd day of pregnancy. The effect of exogenous LH or prolactin (100 ng/ml) on secretion of progesterone (P4) and estradiol (E2) by luteal tissue was studied using perifusion technique. Prolactin caused a significant (P < 0.05) increase in P4 secretion by luteal tissue from gilts in groups I and IV. Both LH and prolactin decreased (P < 0.05) estradiol secretion by luteal tissue obtained from gilts from group IV. Luteinizing hormone stimulated (P < 0.05) P4 and E2 secretion by luteal tissue from gilts from groups IV and II, respectively. The results demonstrate that both LH and prolactin have a regulatory role in steroid secretion by luteal tissue of gilts in the mid- and late periods of pregnancy.
Szafrańska
B
B
Department of Animal Physiology, University of Agriculture and Technology, Olsztyn, Poland.
Grazul-Bilska
A
A
Przała
J
J
D000818:Animals; D001971:Bromocriptine; D003338:Corpus Luteum / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology / Q000502:physiology*; D008659:Metabolic Diseases / Q000139:chemically induced / Q000662:veterinary; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*; D011388:Prolactin / Q000494:pharmacology / Q000502:physiology*; D013552:Swine
D001971:Bromocriptine; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D007986:Luteinizing Hormone