title: [The isolation of highly purified bacillary ribonucleases from recombinant strains of Escherichia coli].
abstract: Active and inactive highly purified extracellular alkaline ribonucleases (barnase) from recombinant Escherichia coli have been prepared according to the industrial technology of Bacillus intermedius ribonuclease purification. Electrophoretic parameters and ultraviolet spectra characterize these preparations as homogeneous proteins. Immunochemical test system of identification of inactive RNAse for its purification has been worked out.
authors.forename: N P
authors.initials: NP
authors.lastname: Balaban
authors.forename: M R
authors.initials: MR
authors.lastname: Sharipova
authors.forename: V I
authors.initials: VI
authors.lastname: Vershinina
authors.forename: L V
authors.initials: LV
authors.lastname: Znamenskaia
authors.forename: E B
authors.initials: EB
authors.lastname: Chernokal'skaia
authors.forename: E R
authors.initials: ER
authors.lastname: Romakhina
authors.forename: T I
authors.initials: TI
authors.lastname: Volkova
authors.forename: S V
authors.initials: SV
authors.lastname: Lopatnev
mesh_terms: D000818:Animals; D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D007114:Immunization; D005779:Immunodiffusion; D010957:Plasmids; D011817:Rabbits; D011995:Recombination, Genetic; D012260:Ribonucleases / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001426:Bacterial Proteins; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease
vernacular_title: Poluchenie vysokoochishchennykh batsilliarnykh ribonukleaz iz rekombinantnykh shtammov Escherichia coli.