[The isolation of nucleases Sm1 and Sm2 in preparative amounts and a study of their antigenicity].
Two enzyme forms of endonuclease (Sm 1 and Sm 2) strain B10M1 in 60 and 100 mg respectively have been isolated from the culture fluid Serratia marcescens. The chromatographic and electrophoretic properties and N-terminal amino acid residues are different for both enzymes. The purification procedure consists of dialysis and ion-exchange chromatography on DEAE- and phosphocellulose. The yield of nucleases Sm1 and Sm2 are 14% and 28% respectively. The antigenic differences of nucleases Sm1 and Sm2 have been found by cross immunoenzyme analysis.
Filimonova
M N
MN
Urazov
N G
NG
Khaertynov
K S
KS
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004706:Endodeoxyribonucleases / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification*; D004722:Endoribonucleases / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification*; D007114:Immunization; D007117:Immunization, Secondary; D007123:Immunoelectrophoresis, Two-Dimensional; D011817:Rabbits; D012706:Serratia marcescens / Q000201:enzymology / Q000276:immunology
D000942:Antigens, Bacterial; D004706:Endodeoxyribonucleases; D004722:Endoribonucleases; C021835:Serratia marcescens nuclease
Vydelenie nukleaz Sm1 i Sm2 v preparativnykh kolichestvakh i izuchenie ikh antigennosti.