Magnetic resonance spectroscopic measurement of cellular thiol reduction-oxidation state.
Chromatographic and magnetic resonance spectroscopic measurements of thiol reduction-oxidation state in chemically constructed samples show close analytical agreement. This result, coupled with the synthesis of new probe molecules allowing greater sensitivity and lower toxicity, supports the development of an NMR method for non-invasive thiol redox measurement, an important variable in the response of tumors to radiation and chemotherapy.
Livesey
J C
JC
University of Washington Medical Center, Seattle 98195.
Golden
R N
RN
Shankland
E G
EG
Grunbaum
Z
Z
Wyman
M
M
Krohn
K A
KA
D004999:Amifostine / Q000031:analogs & derivatives*; D002250:Carbon Radioisotopes; D005978:Glutathione / Q000031:analogs & derivatives; D019803:Glutathione Disulfide; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D011837:Radiation-Protective Agents
D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D011837:Radiation-Protective Agents; C049344:S-2-(3-methylaminopropylamino)ethylphosphorothioic acid; D005978:Glutathione; D004999:Amifostine; D019803:Glutathione Disulfide