[Amplification of a circulating CD4+CD7- T lymphocytes subset in kidney transplantation].
A minor subset of CD4+ T-cells (10 +/- 4 percent) that does not express the CD7 antigen is detectable in normal individuals. These CD4+CD7- T-cells have lower proliferative capacities than autologous CD4+CD7- T-cells. We observed an expansion of CD4+CD7- peripheral T-cells in 45 kidney transplant recipients. The CD4+CD7- TL expansion (23 +/- 20 percent was observed in both short term (< 6 months, n = 20) and long term transplanted patients (> 3 years, n = 25). These results significantly differed from those observed in patients on dialysis (10 +/- 4 percent, P < 0.002). The percentages of CD4+CD7- TL in kidney recipients are significantly correlated with the percentages of CD4+CD57+ and CD8+CD57+ T-cells subsets. A three-color immunofluorescence analysis indicated that 75 percent of the CD4+CD7- TL express the CD57 molecule contrasting with those in normal individuals (0.3 percent). Purified CD4+CD7- T-cells from kidney recipients have lower proliferative responses after PHA, CD2 and CD3 stimulations than autologous CD4+CD7+ TL. The proliferative response is partially restored by the addition of anti-CD28 moAb or IL2. We have highlighted, in kidney recipients with good graft function, an expansion of CD4+CD7- T lymphocytes which could be associated with CD4+ T-cell anergy and tolerance.
Barrou
B
B
Service d'Urologie, Hôpital de la Pitié-Salpêtrière, Paris.
Legac
E
E
Bitker
M O
MO
Jacobs
C
C
Chatelain
C
C
Debré
P
P
Autran
B
B
D015703:Antigens, CD / Q000276:immunology*; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology*; D018959:CD57 Antigens; D016827:CD8 Antigens / Q000276:immunology; D006801:Humans; D016030:Kidney Transplantation / Q000379:methods*; D016176:T-Lymphocyte Subsets / Q000502:physiology*
D015703:Antigens, CD; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D018959:CD57 Antigens; D016827:CD8 Antigens
Amplification d'une sous-population lymphocytaire T circulante CD4+CD7- en transplantation rénale.