title: Antigen F1 from Yersinia pestis forms aqueous channels in lipid bilayer membranes.
abstract: Antigen F1 is a protein of 17 kDa produced by Yersinia pestis when it is cultured at 37 degrees C. When incorporated into planar lipid bilayer membranes this protein induces fluctuations on membrane conductance typical of the formation of ionic channels. These fluctuations reveal two distinct unitary conductance sizes, one in the range of 800 to 1400 pS and the other in the range of 140 to 600 pS. Zero current potential measurements in the presence of a salt gradient show that the channel is not significantly ion selective. The reversal potential measured in the presence of 0.5 M KCl on the cis side and 0.1 M KCl on the trans side was 3.58 +/- 3.98 mV (N = 7). The non-selectivity of the channel, in addition to its large conductance, suggests that it forms large aqueous pores. The present results, taken together with other data showing that antigen F1 inhibits the activity of phagocytic cells, suggest that antigen F1 acts by forming aqueous pores in the membrane of these target cells.
authors.affiliation: Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Brasil.
authors.forename: C G
authors.initials: CG
authors.lastname: Rodrigues
authors.forename: C M
authors.initials: CM
authors.lastname: Carneiro
authors.forename: C T
authors.initials: CT
authors.lastname: Barbosa
authors.forename: R A
authors.initials: RA
authors.lastname: Nogueira
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007473:Ion Channels; D008051:Lipid Bilayers / Q000378:metabolism*; D008970:Molecular Weight; D013696:Temperature; D015010:Yersinia pestis / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D007473:Ion Channels; D008051:Lipid Bilayers