title: Quantification of the c-myc gene in gastric carcinomas by the triplex polymerase chain reaction and high performance liquid chromatography.
abstract: The triplex polymerase chain reaction (PCR) and high performance liquid chromatography (HPLC) techniques were used to examine the state of amplification of the c-myc gene in gastric carcinomas. Sequences from the c-myc gene and from the two control genes were coamplified by PCR. The coamplified PCR products were separated and quantified by HPLC and the copy numbers of the c-myc gene were calculated by comparing the peak areas generated by PCR products. Increased copy numbers of the c-myc gene were found in 2 of 5 patients.
authors.affiliation: Division of Molecular Life Science (Cell Biology), Tokai University School of Medicine, Kanagawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yoshimura
authors.forename: A
authors.initials: A
authors.lastname: Shishikura
authors.forename: S
authors.initials: S
authors.lastname: Koido
authors.forename: S
authors.initials: S
authors.lastname: Ushigome
authors.forename: H
authors.initials: H
authors.lastname: Suemizu
authors.forename: Y
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: T
authors.initials: T
authors.lastname: Moriuchi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004273:DNA, Neoplasm / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D016259:Genes, myc; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm