Whole blood deproteinization for drug screening using automatic pipettors.
Automating the initial screening of postmortem blood and other tissues for drugs of abuse requires a pipetting procedure that is compatible with immunoassay screening tests. The deproteinization procedure uses a zinc sulfate-5-sulfosalicylic acid reagent to precipitate blood proteins. This procedure for deproteinization of whole blood and tissue homogenates is compatible with coated tube radioimmunoassays (RIA) and can therefore be used in automating the initial screening step.
Collins
C
C
Los Angeles County Department of Coroner, California 90033.
Muto
J
J
Spiehler
V
V
D001331:Automation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013287:Illicit Drugs / Q000097:blood*; D010622:Phencyclidine / Q000097:blood; D011180:Postmortem Changes; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods*
D013287:Illicit Drugs; D010622:Phencyclidine