[The effect of chemical agents on lysosome fusion with phagosomes and on the F-actin content in murine peritoneal macrophages].
The influence of natural and synthetic polyamines, phalloidin, cytochalasin D, vinblastine, colchicine, puromycin, chlorpromazine, urea, glutaraldehyde, and ethanol on the phagosome-lysosome fusion and the content of F-actin in murine peritoneal macrophages has been studied. Fluorescent phallotoxin FITC-phalloidin was used to stain F-actin. Natural polyamines (spermine, spermidine, putrescine), phalloidin, ethanol (0.1 M) stimulated the phagosome-lysosome fusion and increased the mid-content of F-actin in macrophages. Cytochalasin D, vinblastine, colchicine, puromycin, chlorpromazine, urea, glutaraldehyde, ethanol (0.15 and 0.2 M) inhibited this process and decreased the mid-content of F-actin. Possible mechanisms of the interconnection of cytoskeleton and the phagosome-lysosome fusion are discussed.
Mozhenok
T P
TP
Rpozanov
Iu M
IuM
Solov'eva
L V
LV
Braun
A D
AD
Bulychev
A G
AG
D000199:Actins / Q000032:analysis / Q000187:drug effects*; D000818:Animals; D002459:Cell Fusion; D002478:Cells, Cultured / Q000187:drug effects / Q000648:ultrastructure; D008247:Lysosomes / Q000187:drug effects* / Q000648:ultrastructure; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity / Q000166:cytology; D010588:Phagosomes / Q000187:drug effects* / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
D000199:Actins
Vliianie nekotorykh agentov na sliianie lizosom s fagosomami i na soderzhanie F-aktina v peritoneal'nykh makrofagakh mysheĭ.