Antibody detection ELISAS for malaria diagnosis.
Parasite extracts of Plasmodium falciparum and P. chabaudi and three synthetic peptides from the P. falciparum MSA2 merozoite antigen were tested for suitability as antigens in an antibody detection ELISA using sera from malaria patients in Brisbane. The P. chabaudi extract was superior to P. falciparum extract for detecting P. vivax cases, while for P. falciparum cases the two parasite extracts were equivalent. Single peptide antigens were generally less sensitive than parasite extracts; however, peptides G3 and G7 were more sensitive than parasite extracts in detecting first attacks of P. vivax. Examination of isotype specific responses demonstrated that this may be explained by higher IgG responses to these peptides in first than in subsequent P. vivax attacks. Because of the differing antibody specificities in primary and secondary P. falciparum and P. vivax cases, the best sensitivity was achieved by using the combined results of assays with three antigens: P. chabaudi, peptide G3 and peptide G7. The combined sensitivity was 77.1% for P. falciparum and 88.6% for P. vivax acute cases with 91.1% specificity.
Graves
P M
PM
Queensland Institute of Medical Research, Bancroft Centre, Herston, Brisbane, Australia.
Boreham
R
R
Robert
G
G
Fray
L
L
Xu
L J
LJ
Huang
Y M
YM
Relf
W
W
Saul
A
A
Kidson
C
C
D000595:Amino Acid Sequence; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000276:immunology; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000276:immunology; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M