Post-column derivatization and fluorimetric detection for the liquid chromatographic analysis of the potential memory-enhancing agent CL 275,838 in human plasma.
A chromatographic method for quantifying the potential memory-enhancing agent CL 275,838 (I) in human plasma with a limit of detection of 1.25 ng/ml is described. The procedure relies on isolation of the compounds from plasma constituents using the Sep-pack C18 cartridge, resolution by reverse-phase high pressure liquid chromatography (HPLC) and post-column oxidation of the eluate peak to from a derivative which can be measured by fluorescence detection. Peak height and compound I concentration were linearly related from 1.25 to 25 ng/ml. Intra- and inter-day validation studies indicated an acceptable precision and reproducibility of the method within the concentration range investigated, the overall coefficient of variation being less than 15%.
Guiso
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Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
Confalonieri
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Caccia
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Kelly
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RG
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D008568:Memory / Q000187:drug effects*; D010084:Oxidation-Reduction; D010879:Piperazines / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology; D011720:Pyrazoles / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology; D011743:Pyrimidines / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology; D011786:Quality Control; D012997:Solvents; D013050:Spectrometry, Fluorescence
D010879:Piperazines; D011720:Pyrazoles; D011743:Pyrimidines; D012997:Solvents; C074871:CL 275838