title: Detection of IgE antibodies specific for 1-phenyl-2,3-dimethyl-3-pyrazoline-5-one by RAST: a serological diagnostic method for sensitivity to pyrazoline drugs.
abstract: Certain adverse reactions to pyrazoline drugs resemble IgE-mediated hypersensitivity. However, convincing evidence of antigen-antibody interactions is not fully demonstrated. In this study, IgE antibodies specific for 1-phenyl-2,3-dimethyl-3-pyrazoline-5-one have been found in 17 out of 19 serum samples from individuals sensitive to pyrazoline drugs with 4-aminoantipyrine discs by Radio Allergo Sorbent Test (RAST). In contrast, we have not found any positive results from 10 normal donors without sensitivity to pyrazoline drugs after ingestion of metamizol 500 mg/day for 14 days. Therefore, our results provide further evidence in favor of an IgE-dependent mechanism in patients suffering from sensitivity to pyrazoline drugs. The determination of specific IgE antibodies could be used as a serodiagnostics method.
authors.affiliation: Department of Allergy, Xuan-Wu Hospital, Capital Institute of Medicine, Beijing, China.
authors.forename: D
authors.initials: D
authors.lastname: Zhu
authors.forename: W M
authors.initials: WM
authors.lastname: Becker
authors.forename: K H
authors.initials: KH
authors.lastname: Schulz
authors.forename: K
authors.initials: K
authors.lastname: Schubeler
authors.forename: M
authors.initials: M
authors.lastname: Schlaak
mesh_terms: D000328:Adult; D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects; D000983:Antipyrine / Q000009:adverse effects / Q000031:analogs & derivatives / Q000276:immunology*; D004177:Dipyrone / Q000009:adverse effects; D004342:Drug Hypersensitivity / Q000175:diagnosis* / Q000209:etiology; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D008024:Ligands; D008297:Male; D008875:Middle Aged; D011852:Radioallergosorbent Test / Q000379:methods*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D008024:Ligands; D007073:Immunoglobulin E; D004177:Dipyrone; C009077:propyphenazone; D000983:Antipyrine