title: Mutations induced by DNA lesions in hot spots of the c-Ha-ras gene.
abstract: In order to investigate whether several DNA lesions (O6-methylguanine, 8-hydroxyguanine, xanthine, an abasic site analogue and hypoxanthine) activate a c-Ha-ras gene and to determine the type of mutations induced by the DNA lesions, they were introduced into a synthetic c-Ha-ras gene by DNA cassette mutagenesis techniques. The modified genes were transfected into mouse NIH3T3 cells and the c-Ha-ras genes present in transformed cells were analysed. O6-methylguanine and xanthine induced a mutation to A, hypoxanthine induced a mutation to G. 8-hydroxyguanine and the abasic site analogue caused random mutations in the modified and adjacent positions. These results indicated that the synthetic c-Ha-ras gene is very useful for the detection of mutations caused by a DNA lesion.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kamiya
authors.forename: H
authors.initials: H
authors.lastname: Miura
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: N
authors.initials: N
authors.lastname: Murata
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: Y
authors.initials: Y
authors.lastname: Komatsu
authors.forename: T
authors.initials: T
authors.lastname: Murata
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: H
authors.initials: H
authors.lastname: Inoue
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004249:DNA Damage; D011905:Genes, ras; D006147:Guanine / Q000031:analogs & derivatives / Q000737:chemistry; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D019820:Xanthine; D014970:Xanthines / Q000737:chemistry
chemical_list: D007042:Hypoxanthines; D014970:Xanthines; D019820:Xanthine; D019271:Hypoxanthine; C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA; C008449:O-(6)-methylguanine