title: Cloning of a type I keratin from goldfish optic nerve: differential expression of keratins during regeneration.
abstract: We report the cDNA sequence and predicted amino acid sequence of a novel type I keratin, designated as GK50, and show that keratin expression in the goldfish optic nerve is highly complex. The GK50 protein is one of at least three type I keratins expressed in goldfish optic nerve based on both antibody reactivity and blot-binding to the type II keratin ON3. After optic nerve crush in situ hybridization shows a localized increase in GK50 mRNA expression in the crush zone. This is in contrast to ON3 mRNA which shows a localized increase that is limited to the proximal and distal margins of the crush zone, suggesting a diversity of keratin expression in different cell types of the goldfish optic nerve.
authors.affiliation: Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794.
authors.forename: R K
authors.initials: RK
authors.lastname: Druger
authors.forename: E M
authors.initials: EM
authors.lastname: Levine
authors.forename: E
authors.initials: E
authors.lastname: Glasgow
authors.forename: P S
authors.initials: PS
authors.lastname: Jones
authors.forename: N
authors.initials: N
authors.lastname: Schechter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D006054:Goldfish; D007633:Keratins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009416:Nerve Regeneration; D009900:Optic Nerve / Q000737:chemistry* / Q000648:ultrastructure; D011485:Protein Binding; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D012333:RNA, Messenger; D007633:Keratins; D004247:DNA