title: Construction of a mouse yeast artificial chromosome library in a recombination-deficient strain of yeast.
abstract: We have constructed a new generation yeast artificial chromosome (YAC) library from female C57BL/10 mice in a recombination-deficient strain of Saccharomyces cerevisiae carrying a mutation in the RAD52 gene. The YAC library contains 41,568 clones with an average insert size of 240 kilobases, representing a greater than threefold coverage of the mouse genome. Currently, the library can be screened by polymerase chain reaction and we have isolated positive clones at a number of loci in the mouse genome. This rad52 library should enable a long-term assessment of the effect of one of the yeast recombination pathway genes on both, genome-wide YAC clone stability and the frequency of chimaeric clones.
authors.affiliation: Department of Biochemistry and Molecular Genetics, St Mary's Hospital Medical School, London, UK.
authors.forename: F L
authors.initials: FL
authors.lastname: Chartier
authors.forename: J T
authors.initials: JT
authors.lastname: Keer
authors.forename: M J
authors.initials: MJ
authors.lastname: Sutcliffe
authors.forename: D A
authors.initials: DA
authors.lastname: Henriques
authors.forename: P
authors.initials: P
authors.lastname: Mileham
authors.forename: S D
authors.initials: SD
authors.lastname: Brown
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D005260:Female; D015723:Gene Library; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic