Soybean phospholipid dependent reductions in triacylglycerol concentration and synthesis in the liver of fasted-refed rats.
The effect of dietary soybean phospholipid on the activities of hepatic triacylglycerol-synthesizing enzymes was compared with soybean oil in fasted-refed rats. Soybean oil at the dietary level corresponding to 20% but not at 5% fatty acid level (21.2 and 5.3% on weight bases, respectively) significantly decreased liver microsomal diacylglycerol acyltransferase activities measured with the endogenous diacylglycerol substrate. Dietary soybean phospholipid even at the dietary level corresponding to 2% fatty acids (3.4% on weight base) significantly decreased the acyltransferase activities measured with endogenous substrate. The dietary phospholipid further decreased the parameter as the dietary level increased, and at the 5% fatty acid level, it was lower than that obtained with soybean oil at 20% fatty acid level. Soybean oil and phospholipid decreased the diacylglycerol acyltransferase activities measured with the saturating concentration of exogenous dioleoylglycerol substrate only when the activities were expressed in terms of total activity (mumol/min per liver) but to much lesser extents. Dietary phospholipid compared to the oil profoundly decreased not only hepatic triacylglycerol but also microsomal diacylglycerol levels. It was indicated that the availability of microsomal diacylglycerol as the substrate for diacylglycerol transferase is the critical determinant in regulating hepatic triacylglycerol synthesis and concentration in this experimental situation. Alterations in the activities of microsomal glycerol 3-phosphate acyltransferase and of the enzymes in fatty acid synthesis could account for the phospholipid-dependent decrease in the microsomal concentration of this intermediate in triacylglycerol synthesis.
Ide
T
T
Laboratory of Nutrition Chemistry, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, Japan.
Hirabayashi
S
S
Kano
S
S
Sugano
M
M
D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase; D004041:Dietary Fats / Q000494:pharmacology*; D004075:Diglycerides / Q000378:metabolism; D005215:Fasting; D005227:Fatty Acids / Q000096:biosynthesis; D005502:Food; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism; D010743:Phospholipids / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013024:Soybean Oil / Q000494:pharmacology*; D013025:Glycine max; D014280:Triglycerides / Q000096:biosynthesis
D004041:Dietary Fats; D004075:Diglycerides; D005227:Fatty Acids; D010712:Phosphatidic Acids; D010743:Phospholipids; D014280:Triglycerides; D013024:Soybean Oil; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase; D002798:Diacylglycerol Cholinephosphotransferase