Single stranded DNA-vectors for analyzing processing of DNA damage induced by 4-nitroquinoline-1-oxide in prokaryotes and eukaryotes.
Previous studies indicate that single stranded DNA vectors could be used in different organisms to study mutagenesis induced by DNA damaging agents. We applied this approach to study mutagenesis induced by 4NQO lesions. The use of ssDNA, on which the ultimate metabolite of 4NQO (Ac-4HAQO) induces mainly C8-guanine adducts, allowed us to find a correlation between G-transversions and the dGuo-C8-AQO adduct. This correlation was established in two independent assay-systems, based on prokaryotic and eukaryotic cells.
Iannone
R
R
Laboratorio di Mutagenesi, IST, Istituto Nazionale per la Ricerca sul Cancro, CSTA, France.
Campomenosi
P
P
Madzak
C
C
Inga
A
A
Caocci
F
F
Abbondandolo
A
A
Fronza
G
G
Sarasin
A
A
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000235:genetics*; D004926:Escherichia coli; D005057:Eukaryotic Cells / Q000187:drug effects*; D005822:Genetic Vectors; D016296:Mutagenesis; D011387:Prokaryotic Cells / Q000187:drug effects*
D004277:DNA, Single-Stranded; D015112:4-Nitroquinoline-1-oxide