Identification of testis specific calcineurin beta subunit isoform by a monoclonal antibody and detection of a specific six amino acid sequence.
Two isoforms of calcineurin beta subunit(beta 1 and beta 2) were identified in rat testis by a monoclonal antibody Va1. Both beta 1 and beta 2 were recovered in calmodulin binding protein fraction and showed calcium shift on SDS-polyacrylamide gel electrophoresis which is the specific character for EF-hand calcium binding protein. beta 2 showed same apparent molecular weight on SDS-PAGE as that of brain calcineurin beta and was found in wide variety of tissues. beta 1 was shown to have six amino acid polypepeptide sequence and it showed higher molecular weight than brain beta and was specific for testis.
Nishio
H
H
Department of Physiology, Kagawa Medical School, Japan.
Matsui
H
H
Etoh
S
S
Moia
L J
LJ
Tokuda
M
M
Itano
T
T
Hatase
O
O
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D019703:Calcineurin; D002148:Calmodulin-Binding Proteins / Q000032:analysis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007527:Isoenzymes / Q000032:analysis*; D046911:Macromolecular Substances; D008297:Male; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000302:isolation & purification; D010749:Phosphoprotein Phosphatases / Q000032:analysis*; D051381:Rats; D013737:Testis / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D002148:Calmodulin-Binding Proteins; D007527:Isoenzymes; D046911:Macromolecular Substances; D009842:Oligopeptides; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases