Flow cytometric analysis of pig epidermal keratinocytes: effects of tape stripping.
Tape stripping is a dynamic in vivo model for the induction of synchronized keratinocyte proliferation. We investigated the cell kinetics of pig epidermis by DNA-flow cytometric analysis, which was compared with [3H]thymidine incorporation mitotic counts and 2-[3H]-deoxy-D-glucose uptake. The stripping was standardized and confirmed histologically by the observation of complete removal of horny layer. Following the stripping, the proportion of cells in S-phase showed no remarkable change until 12 h. This was followed by a spike-like increase in the S-phase cells, the peak of which was reached at 24 h. This gradually decreased and returned to basal levels by 48 h. The cells in G2/M fraction initially decreased; the lowest value was obtained at 12 h. This was followed by a marked increase in the G2/M fraction, the peak of which was at 36 h. The keratinocytes in G2/M fraction gradually returned to basal levels by 96 h. [3H]Thymidine uptake and mitotic counts were mostly parallel with the data of the flow cytometric analysis, suggesting the latter as being a reliable system for cell kinetic analysis. The glucose uptake initially decreased (at 6 h following the stripping) and then increased at 24 h. Histologically the stripped epidermis regained its horny layer almost completely by 72 h following the stripping; this was occasionally accompanied by a moderate acanthotic change thereafter.
Hashimoto
Y
Y
Department of Dermatology, Asahikawa Medical College, Japan.
Tsutsui
M
M
Iizuka
H
H
D000818:Animals; D002453:Cell Cycle; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D015603:Keratinocytes / Q000166:cytology* / Q000502:physiology; D008954:Models, Biological; D012038:Regeneration; D012867:Skin / Q000166:cytology; D012879:Skin Physiological Phenomena; D013552:Swine
D004247:DNA