Identification of important bases for the self-cleavage activity at two single-stranded regions of genomic HDV ribozyme.
In order to determine important bases at two single-stranded regions [SSrA (726-731 nt) and SSrB (762-766)] derived mainly from secondary structure models in genomic hepatitis delta virus (HDV) ribozyme possessing self-cleavage activity, we have constructed several point mutants at these two regions on the HDV88 molecule (683-770). Among the bases at SSrA and SSrB regions C763 was found to play an essential role during self-cleavage process since substitutions to any other bases viz. A or G or U completely abolished the activity.
Nishikawa
S
S
Fermentation Research Institute, Agency of Industrial Science & Technology, MITI, Tsukuba Science City, Japan.
Suh
Y A
YA
Kumar
P K
PK
Kawakami
J
J
Nishikawa
F
F
Taira
K
K
D001483:Base Sequence; D003698:Hepatitis Delta Virus / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*
D016337:RNA, Catalytic; D012367:RNA, Viral