title: Progesterone stimulates sulfate uptake in subcultured endometrial epithelial cells.
abstract: The effect of progesterone was studied on the sulfate entry in glandular epithelial cells of guinea-pig endometrium subcultured in bicameral chambers on matrix-coated filters in a chemically defined medium. At post-confluency (8 days of subculture), cells were treated with 10 nM estradiol alone or in association with various concentrations of progesterone. Optimal progesterone action was at a 16 h incubation time and a 10 nM hormonal concentration. Progesterone increased in a dose-dependent fashion the sulfate uptake specifically in glandular epithelial cells, preferentially from the basal surface. Progesterone effect on the sulfate uptake occurred only in estradiol-primed epithelial cells and was inhibited by the antiprogestin steroid RU-486. The progesterone-dependent increase in sulfate uptake was inhibited by the inhibitor of anion exchange, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). At physiological sulfate concentrations, progesterone essentially induces a high-affinity DIDS-sensitive transport system.
authors.affiliation: Unité de Recherches de Biochimie Hormonale et des Régulations, INSERM U 198, Besançon, France.
authors.forename: L
authors.initials: L
authors.lastname: Beck
authors.forename: A
authors.initials: A
authors.lastname: Mahfoudi
authors.forename: A
authors.initials: A
authors.lastname: Mularoni
authors.forename: M
authors.initials: M
authors.lastname: Nicollier
authors.forename: G L
authors.initials: GL
authors.lastname: Adessi
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004717:Endometrium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D005109:Extracellular Matrix; D005260:Female; D006168:Guinea Pigs; D007700:Kinetics; D015735:Mifepristone / Q000494:pharmacology; D010969:Plastics; D011374:Progesterone / Q000494:pharmacology*; D013431:Sulfates / Q000378:metabolism*
chemical_list: D016895:Culture Media, Serum-Free; D010969:Plastics; D013431:Sulfates; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D015735:Mifepristone; D011374:Progesterone; D004958:Estradiol; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid