[A trial application of the latex test for evaluation of antibody level in rabbit immune sera].
A latex test was elaborated which served for evaluation of quality of rabbit immune sera for antigen 0 of selected Gram-negative bacteria. Sensitivity and specificity of this test in comparison with passive hemagglutination and immunoenzymatic DOT-ELISA reactions was evaluated. These studies were performed on immune sera for antigen O of Salmonella groups B, C1, C2, D and E, Yersinia pseudotuberculosis and in antigen preparations for above listed microorganisms both in homologous and heterologous systems. It was found that sensitivity of the latex test is 9 to 160 times lower than that of passive hemagglutination and 7 to 307 lower than for DOT-ELISA. Sensitivity of the latex test and passive hemagglutination reaction was evaluated on the basis of results of cross reaction between studied antigens and unabsorbed rabbit sera, establishing so called sensitivity indexes, which were informing how many times heterologous titer is lower than homologous titer. So evaluated sensitivity of the latex test was close to sensitivity of the passive hemagglutination reaction. It was found that slide latex test is characterized by satisfactory sensitivity and good sensitiveness and may be used for evaluation of antibody level 0 antigens of Salmonella and Yersinia. The value of this test is characterized by high repeatability of results, as well as low work and time-consuming.
Szych
J
J
Zakład Bakteriologii PZH w Warszawie.
Jagielski
M
M
Kałuzewski
S
S
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D007106:Immune Sera / Q000276:immunology*; D007841:Latex Fixation Tests; D019081:O Antigens; D011105:Polyisoprenyl Phosphate Sugars / Q000032:analysis*; D011817:Rabbits; D012475:Salmonella / Q000276:immunology*; D012680:Sensitivity and Specificity; D015007:Yersinia / Q000276:immunology*
D000907:Antibodies, Bacterial; D007106:Immune Sera; D019081:O Antigens; C062656:O-specific polysaccharide, Salmonella; D011105:Polyisoprenyl Phosphate Sugars
Próba wykorzystania odczynu lateksowego do oceny poziomu przeciwciał w króliczych surowicach odpornościowych.