title: Interaction between endothelial cells and thalassemic red cells in vitro.
abstract: Erythrocytes from 45 patients with thalassemia and/or hemoglobinopathies were studied for their cytoadherence property to the vascular endothelial cells in vitro. In plasma free medium, erythrocytes from patients with beta-thal/Hb E both splenectomized and nonsplenectomized, HbH diseases (alpha-thal 1/alpha-thal 2 and alpha-thal 1/Hb Constant Spring genotypes) and homozygous Hb E subjects bind to endothelial cells at a greater number as compared to the binding cell number of normal erythrocytes (p-value < 0.05 in all types). Addition of autologous platelet-rich plasma or whole blood to the culture system causes further increase in the number of adhering beta-thalassemia red cells. Platelet-rich plasma had more enhancement effect than the whole blood. However, no such enhancement of both platelet-rich plasma and whole blood was demonstrated in the culture of normal or alpha-thalassemia erythrocytes. Increased binding between red cells and endothelial cells may contribute to the greater risk of vascular occlusion in thalassemic patients.
authors.affiliation: Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Butthep
authors.forename: A
authors.initials: A
authors.lastname: Bunyaratvej
authors.forename: H
authors.initials: H
authors.lastname: Kitaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Funahara
authors.forename: S
authors.initials: S
authors.lastname: Fucharoen
mesh_terms: D001792:Blood Platelets / Q000502:physiology; D001809:Blood Viscosity; D002448:Cell Adhesion / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D004913:Erythrocytes, Abnormal / Q000502:physiology*; D005838:Genotype; D006446:Hemoglobin E; D006453:Hemoglobinopathies / Q000097:blood* / Q000235:genetics / Q000601:surgery; D006455:Hemoglobins, Abnormal; D006785:Hospitals, University; D006801:Humans; D013156:Splenectomy; D013785:Thailand; D017085:alpha-Thalassemia / Q000097:blood* / Q000235:genetics / Q000601:surgery; D017086:beta-Thalassemia / Q000097:blood* / Q000235:genetics / Q000601:surgery
chemical_list: D006455:Hemoglobins, Abnormal; D006446:Hemoglobin E; C035541:Hemoglobin Constant Spring