title: Syntheses and properties of fluorescent labeled oligonucleotides containing deoxyethenoadenosine at 5' end.
abstract: The fluorescent labeled oligodeoxyribonucleotides which contain deoxyethenoadenosione (d epsilon A) at their 5' end were prepared by treating CPG bound oligonucleotides with 5'-DMTr-deoxyethenoadenosine-3'-H-phosphonate. The hybrid formation of d epsilon A-oligonucleotide with its complementary DNA was studied by fluorescence spectroscopy. The fluorescence of d epsilon A in a single strand was largely quenched by stacking interaction with the base at 3' position. When d epsilon A-oligonucleotides hybridized with their complementary strands, relative fluorescence quantum yields (Qrel) against d epsilon A changed in specific manners. These results suggest that d epsilon A-oligonucleotides are applicable to study the local structure of DNA in solution.
authors.affiliation: Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Otoi
authors.forename: N
authors.initials: N
authors.lastname: Fujimura
authors.forename: T
authors.initials: T
authors.lastname: Morii
authors.forename: H
authors.initials: H
authors.lastname: Ide
authors.forename: K
authors.initials: K
authors.lastname: Makino
mesh_terms: D001483:Base Sequence; D003839:Deoxyadenosines / Q000737:chemistry*; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D003839:Deoxyadenosines; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; C032221:1,N(6)-ethenodeoxyadenosine