Blood grouping of minute samples using monoclonal anti-A,B antibody.
The availability of a monoclonal anti-A,B antibody (AB-E6E2) for blood grouping of blood and saliva stains was investigated. The antibody, which was reacted with A, B and AB antigens but not with O antigen, was produced from BALB/c mouse-mouse hybridoma raised to group AB red blood cells. AB-E6E2 had heat-elutability enough to detect ABO antigens in blood and saliva stains by absorption-elution test, whereas it had extremely weak double combining potency in mixed agglutination test. Application of elution-ELISA method will enable the detection of smaller amount of ABH antigens. The antibody, therefore, would be useful to segregate group O minute samples from the other blood group ones.
Aoki
Y
Y
Department of Forensic Medicine, Tohoku University School of Medicine, Sendai, Japan.
Nagae
M
M
Nata
M
M
Sagisaka
K
K
D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal; D001801:Blood Stains; D004797:Enzyme-Linked Immunosorbent Assay; D005554:Forensic Medicine; D006801:Humans
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal