[Effect of cyclosporin A, azathioprine, and methylprednisolone on plasma levels and heart atrial content of atrial natriuretic peptide in the rat].
In Wistar normotense male rats of six months old the effect of Cyclosporine A (Cys A), azathioprine (AZA) and methyl prednisolone (M-Pred), alone or associated, on the plasmatic levels and atrial content of the atrial natriuretic peptide (PNA) have been studied. The values of creatinine, urea, sodium a potassium were also measured in serum. This treatment was maintained for 30 consecutive days at these doses: Cys A (5.00 mg/kg b.w./day), AZA (2.50 mg/kg b.w/day) and M-Pred (20.00 mg/kg b.w./day), being the peritoneal the way of administration. Eight groups were organized: control, vehicle, Cys A, AZA, M-Pred, Cys A + M-Pred, AZA + M-Pred, Cys A + AZA + M-Pred. All the above parameters were estimated at 24 hours after respective injections. No changes are found after Cys A or AZA in the plasmatic and atria contents of PNA. Nevertheless, m-Pred alone or in association determines high increases of PNA in plasma and tissue. The other parameters are unchanged. The data reveal the beneficial use of M-Pred, glucocorticoid of the low mineral activity, in the therapy of rejection in cardiac transplantation. On the other hand, Cys A and AZA appear not to be important in the secretion and atrial synthesis of PNA.
Montilla
P
P
Dipartimento di Biochimica, Facoltà di Medicina, Università di Cordoba (Spagna).
Muñoz
J L
JL
Montilla
M C
MC
Muñoz
M C
MC
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis*; D001379:Azathioprine / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D003404:Creatinine / Q000097:blood; D016572:Cyclosporine / Q000494:pharmacology*; D004357:Drug Synergism; D006325:Heart Atria / Q000737:chemistry / Q000187:drug effects*; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology*; D009318:Natriuresis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar / Q000378:metabolism; D014508:Urea / Q000097:blood
D016572:Cyclosporine; D009320:Atrial Natriuretic Factor; D014508:Urea; D003404:Creatinine; D001379:Azathioprine; D008775:Methylprednisolone
Effetto della ciclosporina A, dell'azatioprina e del metil-prednisolone sui livelli plasmatici e sul contenuto negli atri cardiaci di peptide natriuretico atriale nel ratto.