[DNA typing and analysis of the D1S8 (MS 32) allele in the Japanese population by the minisatellite variant repeat (MVR) mapping by polymerase chain reaction (PCR) assay].
Minisatellite variant repeat (MVR) mapping using the polymerase chain reaction (PCR) of the D1S8 (MS32) locus from total genomic DNA and size separated alleles in the Japanese population was performed. DNA was extracted from blood in healthy non-related Japanese individuals. The digital data obtained were computer analysed, and from this single assay all unrelated individuals tested could be unambiguously distinguished. The average difference in diploid codes between unrelated individuals was 36 exclusions over the first 60 repeat unit positions. The mean proportions of a-, t-, and 0-type repeat units were 73.5%, 24.5% and 2.1%, respectively. MVR-PCR gives digital profiles which show extreme levels of individual variation and should prove very useful for personal identification. The advantages and further applications of MVR-PCR are also discussed.
Tamaki
K
K
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
Yamamoto
T
T
Uchihi
R
R
Kojima
T
T
Katsumata
Y
Y
Jeffreys
A J
AJ
D000483:Alleles; D044466:Asian People; D001483:Base Sequence; D004276:DNA, Satellite / Q000235:genetics*; D005815:Genetic Code; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004276:DNA, Satellite