In vitro angiogenesis in fibrin matrices containing fibronectin or hyaluronic acid.
Angiogenesis involved numerous interactions between extracellular matrix and endothelial cells which may exhibit changes in actin filament distribution. Using an in vitro model, capillary endothelial cells were grown in fibrin matrix containing fibronectin or hyaluronic acid. Actin filament distribution, nucleus localization and cell morphology were observed. Preliminary study showed the formation of tube-, branche- and capillary-like structures within fibrin. In the presence of both fibrin and fibronectin, cells with actin filament stress fibers were more spreading than those in fibrin. In the presence of hyaluronic acid, tubes were limited in extension into the fibrin. In addition, the study of co-localization of nucleus and actin filaments showed different cell behaviours. Migratory cells seem to arrange in parallel to each other and a capillary-like structure may be formed at the proximal extremity of this cell pattern.
Fournier
N
N
Laval University, Québec, Canada.
Doillon
C J
CJ
D000199:Actins / Q000032:analysis; D002196:Capillaries / Q000254:growth & development; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000254:growth & development*; D005337:Fibrin; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007150:Immunohistochemistry; D009389:Neovascularization, Pathologic / Q000503:physiopathology*
D000199:Actins; D005353:Fibronectins; D005337:Fibrin; D006820:Hyaluronic Acid