[The EGF receptor pathway in human cerebral tumors].
The epidermal growth factor receptor gene is the most frequently involved proto-oncogene in human glial brain tumors, in the present series in agreement with previous reports in literature. It is therefore important to study this gene from DNA to the protein product. The vicinity of cystic fluid (C.F.) to tumor cells of the cystic wall has suggested investigation of possible "E.G.F.-like" autocrine activities in C.F. In 40% of gliomas, E.G.F.-R. gene is amplified and overexpressed. This is never observed in low grade astrocytomas. In 12% of the cases, mutations of the E.G.F.-R. gene are observed. In correlation with genomic abnormalities, E.G.F.-R. is immunoprecipitated in 40% gliomas. The basal phosphorylation of the receptor is increased in 50% gliomas. In C.F., unexpectedly, E.G.F.-R. phosphorylation inhibitory effect is observed. Its biochemical analysis suggests an anti-tyrosine kinase activity. The observation of anti-tyrosine kinase activity in C.Fs suggests the presence of negative modulatory factors of the proto-oncogene activation in tumor tissues. This could have therapeutical interest.
Berger
F
F
Unité INSERM 318, Laboratoire de Neurobiophysique, Université Joseph Fourier, C.H.U.R. de Grenoble.
Laine
M
M
Hoffmann
D
D
Verna
J M
JM
Charffanet
M
M
Chauvin
C
C
Rost
N
N
Nissou
M F
MF
Benabid
A L
AL
D015152:Blotting, Northern; D015139:Blotting, Southern; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000737:chemistry* / Q000235:genetics; D066246:ErbB Receptors / Q000032:analysis* / Q000235:genetics; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D010766:Phosphorylation; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes / Q000235:genetics
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D066246:ErbB Receptors
Etude de la voie du récepteur a l'E.G.F. dans les tumeurs cérébrales humaines.