[Growth factor from the tissues of the marine mollusk Mytilus edulis].
The absence of specific stimulators of cell proliferation, i.e. of growth factors, is one of the causes of failure in long-term cultivation of the marine invertebrate cells. In search for such stimulators in tissues of marine invertebrates, we succeeded in discovering in some tissues a stimulator of cell proliferation, similar to EGF, with a comparatively high content of it in tissues of Mytilus edulis. The similarity of the obtained factor with EGF was shown by the substitution of 125I-EGF, connected with EGF receptors on the surface of A431 cells, with analysed extracts, as well as by the ability of this extract to induce the internalization of EGF-receptor complexes. The fraction of acid/ethanol extract of M.edulis stimulating the cell proliferation in resting mouse fibroblasts Swiss 3T3, was obtained in the same conditions as EGF did, using reversed phase chromatography. Thus, the factor from tissues of M.edulis may belong to the family of EGF-like factors.
Odintsova
N A
NA
Nesterov
A M
AM
Korchagina
D A
DA
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D049872:Bivalvia / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D004815:Epidermal Growth Factor / Q000302:isolation & purification / Q000494:pharmacology*; D066246:ErbB Receptors / Q000187:drug effects; D006737:Horseshoe Crabs; D006801:Humans; D007457:Iodine Radioisotopes; D051379:Mice; D012616:Sea Cucumbers; D012617:Sea Urchins; D013215:Starfish; D014407:Tumor Cells, Cultured / Q000187:drug effects
D007457:Iodine Radioisotopes; D004815:Epidermal Growth Factor; D066246:ErbB Receptors
Rostovoĭ faktor iz tkaneĭ morskogo molliuska Mytilus edulis.