[Chemical-enzymatic synthesis and cloning of the full-length genome of a DNA-containing plant virus].
The full-length genomic DNA of a small plant virus--coconut foliar decay virus (CFDV)--has been synthesized by a combination of chemical synthesis, ligation and polymerase chain reaction. Three separately cloned DNA fragments of the genomic DNA were sequenced and assembled in the pPCV002 plasmid vector.
Chernov
B K
BK
Merits
A
A
Mizenina
O A
OA
Morozov
S Iu
SIu
D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000378:metabolism*; D010957:Plasmids
D004279:DNA, Viral; D004798:Enzymes
Khimiko-fermentativnyĭ sintez i klonirovanie polnorazmernogo genoma DNA-soderzhashchego virusa rasteniĭ.