title: Effects of cholera toxin on human colon carcinoma cell lines.
abstract: This study reports on changes in morphology and membrane transport in 5 human colon carcinoma cell lines treated with cholera toxin (CT). Three of the cell lines that grew as monolayers (LIM 1215, LIM 1899, LIM 2099) and 1 that grew as floating clumps (LIM 2408) did not show morphological changes after CT treatment. However, cell line LIM 1863 that grows as floating "crypt-like" organoids showed rapid and distinctive changes in morphology and membrane transport after CT treatment. At 1 and 6 hrs after CT treatment, light and transmission electron microscopy revealed rapid dilatation of the central lumen of organoids and the appearance of 2 populations of apical vesicular inclusions. The first population was unusual in being non-membrane bound and limited by fuzzy filamentous material. The second population was membrane bound. Scanning electron microscopy at 1-6 hr after CT treatment showed swelling and loss of surface microvilli on some, but not all, cells. At 24 hr after CT treatment the majority of organoids showed evidence of fluid accumulation and small apical vesicles coalesced to form large single vacuoles that obliterated normal cell morphology. By 48 hr, continued swelling produced extreme attenuation of the plasma membrane with cells taking on an "endothelial cell-like" appearance. The response to CT was dose-dependent. Uptake studies using 86Rubidium and blocking studies using ouabain and amiloride indicated that CT is acting on the Na+/K+ ATPase membrane pump to cause the increased fluid uptake by LIM 1863 cells. This study is the first to report specific morphological changes in intestine-derived cells in response to CT.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy, Monash University, Victoria.
authors.forename: D H
authors.initials: DH
authors.lastname: Barkla
authors.forename: R H
authors.initials: RH
authors.lastname: Whitehead
authors.forename: I P
authors.initials: IP
authors.lastname: Hayward
mesh_terms: D002277:Carcinoma / Q000473:pathology* / Q000648:ultrastructure; D002772:Cholera Toxin / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D002772:Cholera Toxin