[Nuclease conjugates with ligand-free serum albumin].
In order to obtain nuclease and human serum albumin (HSA) conjugates with a high enzyme content it is proposed to use a ligand-free HSA. The ligands are removed with the help of a strong anion exchanger. A two-stage procedure of conjugate preparation is proposed. It consists in the complexation of ligand-free HSA and enzyme and subsequent co-condensation of protein molecules of the poly-complex with the aid of glutaric aldehyde. When the conjugates are administered to rabbits intravenously, the RNAase activity is manifested in blood for 3-5 days. Moreover, in the case of conjugates with a molecular weight of 80 kDa, the prolongation time is greater than for conjugate with a higher molecular weight.
Ponomareva
R B
RB
Zelepuga
E A
EA
Medvedev
M L
ML
D000818:Animals; D001407:Bacillus / Q000201:enzymology; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005976:Glutaral; D006801:Humans; D008024:Ligands; D008970:Molecular Weight; D010179:Pancreas / Q000201:enzymology; D011817:Rabbits; D012260:Ribonucleases / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry*; D012709:Serum Albumin / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D013997:Time Factors
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D008024:Ligands; D012709:Serum Albumin; D012260:Ribonucleases; D005976:Glutaral
Kon''iugaty nukleaz s bezligandnym syvorotochnym al'buminom.