A method for thin sectioning heavily sclerotized cuticle of Odontotaenius disjunctus.
Elytra of the bessbeetle, Odontotaenius disjunctus were thin sectioned after embedding in epoxy resin. Sections were cut with a diamond saw, ground to the desired thickness on a rotary grinder and polished. Tearing and distortion were reduced when compared to knifecut sections of heavily sclerotized cuticle.
Sawvel
J W
JW
Dept. of Biological Sciences, Ohio Northern University, Ada 45810.
Gunderson
S
S
Schiavone
R C
RC
Nelson
E V
EV
D000818:Animals; D001517:Coleoptera / Q000648:ultrastructure*; D004853:Epoxy Resins; D006586:Hexanes; D008867:Microtomy / Q000379:methods*; D016613:Plastic Embedding; D016707:Tissue Fixation
D004853:Epoxy Resins; D006586:Hexanes; C026385:n-hexane