title: Identification and characterization of foot-and-mouth disease virus O1 Burgwedel/1987 as an intertypic recombinant.
abstract: The foot-and-mouth disease virus field isolate Burgwedel/1987 subtype O1 was found to differ genetically from the antigenically related strain O1 Kaufbeuren within the region encoding the non-structural proteins. This genetic difference was indicated by the RNase mismatch cleavage method and confirmed by nucleotide sequencing. An alignment of sequences encoding proteinase 3C of the Burgwedel isolate and several other virus strains identified this isolate as an intertypic recombinant; the parent strains were O1 Kaufbeuren and a subtype C1 strain. Recombination occurred between nucleotide positions 5493 and 5521, within the region encoding peptide 3B1. Thus, the 5' three-quarters of the O1 genome were fused to the 3'-terminal quarter of the C1 genome. Other contemporary isolates from the same district are not recombinants. Sequence alignment distinguished four patterns of proteinase 3C-coding sequences among the virus strains analysed: subtypes A12, C1 and O1 exhibit one pattern each, and another pattern is common to subtypes A5, A10 and O2.
authors.affiliation: Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Krebs
authors.forename: O
authors.initials: O
authors.lastname: Marquardt
mesh_terms: D005537:Aphthovirus / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002874:Chromosome Mapping; D003434:Crossing Over, Genetic; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012367:RNA, Viral