[The use of flow cytofluorometry for determining antisperm antibodies by sperm immobilization and sperm toxicity methods].
During antisperm antibody detection by conventional methods of immobilization and cytotoxicity investigators used flow cytometry to detect dead and/or immovable sperm cells. It was shown that after incubation of sperm cells with low antiserum dilution immovable spermatozoa are still living. Evidently, cytotoxicity test in this case brings about false-negative results. Therefore, flow cytometry will be a success for ASAB detection through immobilization test.
Nikolaeva
M N
MN
Verbitskiĭ
M Sh
MSh
Sukhikh
G T
GT
D000818:Animals; D000906:Antibodies / Q000097:blood*; D016923:Cell Death / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D011817:Rabbits; D013081:Sperm Motility / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*
D000906:Antibodies
Ispol'zovanie protochnoĭ tsitofliuorometrii dlia opredeleniia antispermal'nykh antitel metodami spermoimmobilizatsii i spermotoksichnosti.