Allotypes of the fourth component of complement in Koreans.
The analysis of genetic polymorphism in C4 was performed on EDTA-plasma from 169 healthy unrelated Koreans. Plasma samples were subjected to high-voltage agarose gel electrophoresis followed by immunofixation. C4B allotypes were further detected by a hemolytic overlay method. The allele frequencies of C4A and C4B were as follows; for C4A, C4A*3 = 0.6099, C4A*4 = 0.1702, C4A*Q0 = 0.1525, C4A*2 = 0.0461, and C4A*R = 0.0213; for C4B, C4B*1 = 0.6406, C4B*2 = 0.2740, C4B*5 = 0.0569, C4B*Q0 = 0.0178, and C4B*R = 0.0107. C4A*3 and C4B*1 were among the most common alleles at each locus. C4A*6 was not detected in this study, but this allele is relatively common in both Caucasoid and Negroid populations. C4B*5 is a common allele in Asian, which is rare in Caucasoids and Negroids. C4B*5 appeared to be a characteristic allele of Oriental. In the C4A locus, five individuals with duplicated allotypes (three C4A 3,3 + 2, one C4A 4,3 + 2, and one C4A 3,3 + 3) were observed, and in the C4B locus, one individual with duplicated allotype (C4B 1,1 + 1) was detected.
Park
K S
KS
Department of Biology, Sung Shin Women's University, Seoul, Korea.
Choi
S Y
SY
Park
M H
MH
Tokunaga
K
K
D000483:Alleles; D044466:Asian People / Q000235:genetics*; D015934:Complement C4a / Q000235:genetics*; D015935:Complement C4b / Q000235:genetics*; D015941:Complement Hemolytic Activity Assay; D004587:Electrophoresis, Agar Gel; D005787:Gene Frequency; D006801:Humans; D007723:Korea; D011110:Polymorphism, Genetic
D015934:Complement C4a; D015935:Complement C4b