Protoplast fusion of beta-glucosidase-producing Aspergillus niger strains.
Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 x 10(-2) - 9.1 x 10(-2). After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and beta-glucosidase activities. One segregant showed a 2.5-fold increase in beta-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains of A. niger with improved beta-glucosidase activities.
Hoh
Y K
YK
Department of Botany, Faculty of Science, National University of Singapore.
Tan
T K
TK
Yeoh
H H
HH
D001234:Aspergillus niger / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D002118:Calcium / Q000494:pharmacology; D007700:Kinetics; D011092:Polyethylene Glycols / Q000494:pharmacology; D011523:Protoplasts / Q000502:physiology; D001617:beta-Glucosidase / Q000302:isolation & purification / Q000378:metabolism*
D011092:Polyethylene Glycols; D001617:beta-Glucosidase; D002118:Calcium