Localization of basic fibroblast growth factor (bFGF) in a metastatic cell line (AT-3) established from the Dunning prostatic carcinoma of rat: application of a specific monoclonal antibody.
Localization of basic fibroblast growth factor (bFGF) in a metastatic cell line, AT-3, established from the Dunning prostatic carcinoma of rat was determined by two immunological techniques using a specific monoclonal antibody against bFGF. Concentration of bFGF in cell extract was measured by sandwich radioimmunoassay (RIA) with heparin-Sepharose and 125I-labeled monoclonal antibody. bFGF concentration in the extract of AT-3 cells increased with increasing concentration of NaCl in extraction buffer. Localization of bFGF in AT-3 cells was determined by counting radioactivity of 125I-labeled monoclonal antibody binding to AT-3 cells before or after increasing permeability of the cells. The binding increased significantly by this treatment, indicating that bFGF within the cells was detected.
Nishikawa
K
K
Department of Biochemistry, Kanazawa Medical University, Ishikawa, Japan.
Yoshitake
Y
Y
Minemura
M
M
Yamada
K
K
Matuo
Y
Y
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002455:Cell Division / Q000187:drug effects; D002463:Cell Membrane Permeability; D004261:DNA Replication / Q000187:drug effects; D016220:Fibroblast Growth Factor 1 / Q000096:biosynthesis; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000473:pathology*; D011863:Radioimmunoassay; D051381:Rats; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D009363:Neoplasm Proteins; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1