[A method for acquiring newborn larvae of Trichinella spiralis in vitro].
This paper reports a simple method for acquiring numerous newborn larvae of Trichinella spiralis in vitro. Adults of T. spiralis, collected from small intestine on rats which were infected with infective larvae separated from mice infected experimentally, were put into tissue culture bottle containing M199. Then the bottles were incubated in CO2 incubator (37 degrees C, 5% CO2) for 15-20h. The newborn larvae were collected by passing through filtration, centrifugation and repeated wash. The results showed that this method is effective in terms of the activity of the adults and newborn larvae and the amount of larvae gained. The method in economical and can control bacterial contamination to a certain extent and extract ES antigen of adults and newborn larvae from the culture solution.
Zhang
R
R
Department of Parasitology, North Sichuan Medical College.
Feng
R
R
D000818:Animals; D003470:Culture Media; D007421:Intestine, Small / Q000469:parasitology; D007814:Larva; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D017160:Trichinella spiralis / Q000254:growth & development / Q000302:isolation & purification*; D014235:Trichinellosis / Q000469:parasitology
D003470:Culture Media