Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.
A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited number of sites in the membrane. A qualitative study shows that an increase in the labelling leads to an evolution of the probe fluorescence properties; the existence of different types of sites is involved in the interpretation but the system is too complex to allow it to be represented by a simple model. As a consequence of this study, care has to be taken in labelling biological material so as to avoid excessive probe incorporation.
Maire
C
C
LA 328 du CNRS, ENSIC, Nancy, France.
Bouchy
M
M
Donner
M
M
André
J C
JC
D004161:Diphenylhexatriene / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D006801:Humans; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological
D005456:Fluorescent Dyes; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene