Site-specific deletion and rearrangement of integron insert genes catalyzed by the integron DNA integrase.
Deletion of individual antibiotic resistance genes found within the variable region of integrons is demonstrated. Evidence for gene duplications and rearrangements resulting from the insertion of gene units at new locations is also presented. Deletion, duplication, and rearrangement occur only in the presence of the integron-encoded DNA integrase. These events are precise and involve loss or gain of one or more complete insert units or gene cassettes. This confirms the recent definition of gene cassettes as consisting of the gene coding sequences, all except the last 7 bases of the 59-base element found at the 3' end of the gene, and the core site located 5' to the gene (Hall et al., Mol. Microbiol. 5:1941-1959, 1991) and demonstrates that individual gene cassettes are functional units which can be independently mobilized. Both deletions and duplications can be generated by integrase-mediated cointegrate formation followed by integrase-mediated resolution involving a different pair of sites. However, deletion occurs 10 times more frequently than duplication, and we propose that the majority of deletion events are likely to involve integrase-dependent excision of the gene unit to generate a circular gene cassette. The implications of these findings in understanding the evolution of integrons and the spread of antibiotic resistance genes in bacterial populations is discussed.
Collis
C M
CM
Division of Biomolecular Engineering, Commonwealth Scientific and Industrial Research Organisation, North Ryde, New South Wales, Australia.
Hall
R M
RM
D001483:Base Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004251:DNA Transposable Elements / Q000235:genetics*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D015321:Gene Rearrangement; D019426:Integrases; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D000198:Spectinomycin / Q000378:metabolism; D014162:Transfection / Q000235:genetics*
D004251:DNA Transposable Elements; D000198:Spectinomycin; D004254:DNA Nucleotidyltransferases; D019426:Integrases