[Biochemical and immunochemical analyses of the protein components of the cell wall in group-A streptococci isolated by a sparing chemical method].
To study the protein components of the cell wall of group A streptococci, type M 29, a special preparative method was developed (extraction with 1 M hydroxylamine solution, pH 6.0, and subsequent purification). Altogether six protein fractions were obtained. The isolated proteins were found to be a heterogeneous group of molecules, consisting of 25-40 individual proteins with molecular weights ranging between 13 and 94 kD. The study of the protein fractions thus obtained in the immunodiffusion test with rabbit antiserum to the initial protein preparation revealed that these proteins contained type-specific components, 3-6 type-nonspecific protein antigens common with protein antigens of M 1 and M 12, as well as one protein antigen common with type M 1. Fc receptor was shown to be absent. The detected type-nonspecific protein antigens were partially separated by ion-exchange chromatography and some of them could be purified from the admixtures of nucleic acids and group-specific polysaccharide.
Blinnikova
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EI
Shikhman
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AR
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002458:Cell Fractionation / Q000379:methods; D002473:Cell Wall / Q000737:chemistry* / Q000276:immunology; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D000939:Epitopes / Q000032:analysis; D007114:Immunization; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D011817:Rabbits; D011961:Receptors, Fc / Q000032:analysis; D013297:Streptococcus pyogenes / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D011961:Receptors, Fc
Biokhimicheskiĭ i immunokhimicheskiĭ analizy belkovykh komponentov kletichnoĭ stenki streptokokkov gruppy A, vydelennykh shchadiashchim khimicheskim metodom.