[Genetically engineered human insulin. 1. HPLC in analyzing products of basic production stages].
Application of some variants of HPLC for the step-by-step analysis of recombinant human insulin production was studied. Chromatographic columns with commercial and specially developed supports for size-exclusion, ion-exchange and reverse phase HPLC were used. Effective combinations of the chromatographic techniques for analysis of products and intermediates at every technological step were found and used for production of insulin. The authenticity of insulin obtained in the Shemyakin Institute of Bio-organic Chemistry by the scheme described in the present paper was confirmed by means of some physical and chemical methods and biological activity analysis.
Kliushnichenko
V E
VE
Iakimov
S A
SA
Mal'tsev
K V
KV
Arutiunian
A M
AM
Ivanov
A E
AE
Vul'fson
A N
AN
D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006801:Humans; D007328:Insulin / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics
D007328:Insulin; D011994:Recombinant Proteins
Genno-inzhenernyĭ insulin cheloveka. 1. VEZhKh v analize produktov osnovnykh stadiĭ proizvodstva.