Hippocampal kindling increases the expression of glutamate receptor-A Flip and -B Flip mRNA in dentate granule cells.
The level of the mRNAs encoding the AMPA-selective glutamate receptors-A and -B, alternatively splice variants, Flip and Flop, was studied by in situ hybridization in the brains of rats kindled by Schaffer collateral/commissural-fiber stimulation. In comparison to control animals, the expression level of the Flip variant of both GluR-A and GluR-B mRNAs was bilaterally enhanced in the dentate granule neurons of kindled animals 24 h after last-generalized seizure, whereas no obvious alterations were observed in the GluR-A Flop and GluR-B Flop mRNA variants. In kindled animals, studied 1 month after the last seizure, GluR-A Flip and GluR-B Flip mRNA had returned to control levels. We suggest that these changes may result in an enhanced glutamate receptor sensitivity in the fascia dentata during kindling.
Kamphuis
W
W
Department of Experimental Zoology, University of Amsterdam, The Netherlands.
Monyer
H
H
De Rijk
T C
TC
Lopes da Silva
F H
FH
D017398:Alternative Splicing; D000818:Animals; D004558:Electric Stimulation; D005071:Evoked Potentials; D014644:Genetic Variation; D006624:Hippocampus / Q000166:cytology / Q000502:physiology* / Q000503:physiopathology; D007696:Kindling, Neurologic; D008297:Male; D011712:Pyramidal Tracts / Q000166:cytology / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000096:biosynthesis / Q000235:genetics*; D012640:Seizures / Q000503:physiopathology; D013997:Time Factors
D012333:RNA, Messenger; D017470:Receptors, Glutamate