title: Single amino acid substitutions can further increase the stability of a thermophilic L-lactate dehydrogenase.
abstract: Lactate dehydrogenases are of considerable interest as stereospecific catalysts in the chemical preparation of enantiomerically pure alpha-hydroxyacid synthons. For such applications in synthetic organic chemistry it would be desirable to have enzymes which tolerate elevated temperatures for prolonged reaction times, to increase productivity and to extend their applicability to poor substrates. Here, two examples are reported of significant thermostabilizations, induced by site-directed mutagenesis, of an already thermostable protein, the L-lactate dehydrogenase (EC 1.1.1.27, 35 kDa per monomer subunit) from Bacillus stearothermophilus. Thermal inactivation of this enzyme is accompanied by irreversible unfolding of the native protein structure. The replacement of Arg171 by Tyr stabilizes the enzyme against thermal inactivation and unfolding. This stabilizing effect appears to be based on improved interactions between the subunits in the core of the active dimeric or tetrameric forms of the enzyme. The thermal stability of L-lactate dehydrogenase variants with an active site Arg residue, either in the 171 (wild-type) or in the 102 position, is further increased by sulfate ions. The two stabilizing effects are additive, as found for the Arg171Tyr/Gln102Arg double mutant, for which the stability of the protein in 100 mM sulfate solution reaches that of L-lactate dehydrogenases from extreme thermophiles. All mutant proteins retain significant catalytic activity, both in the presence and absence of stabilizing salts, and are viable catalysts in preparative scale reactions.
authors.affiliation: Department of Chemistry, University of Toronto, Ontario, Canada.
authors.forename: H K
authors.initials: HK
authors.lastname: Kallwass
authors.forename: W K
authors.initials: WK
authors.lastname: Surewicz
authors.forename: W
authors.initials: W
authors.lastname: Parris
authors.forename: E L
authors.initials: EL
authors.lastname: Macfarlane
authors.forename: M A
authors.initials: MA
authors.lastname: Luyten
authors.forename: C M
authors.initials: CM
authors.lastname: Kay
authors.forename: M
authors.initials: M
authors.lastname: Gold
authors.forename: J B
authors.initials: JB
authors.lastname: Jones
mesh_terms: D002151:Calorimetry; D004795:Enzyme Stability; D005818:Genetic Engineering; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000235:genetics; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D006358:Hot Temperature; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D011489:Protein Denaturation; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D006146:Guanidines; D011994:Recombinant Proteins; D007770:L-Lactate Dehydrogenase; D019791:Guanidine