Mass culture of anchorage-dependent animal cells with newly born calf skin collagen membrane and microcarrier.
It was shown that collagen substrate enhances the growth as well as the differentiation of many cells in culture. Collagen is one of the major fibrous proteins of animal bodies and the pure collagen used in this experiment was extracted from the newly born calf skin by chemical and biochemical methods. The analytical results obtained by ion-exchange chromatography and electrophoresis showed that the main components of denatured collagen were alpha monomers and beta dimers. The collagen and denatured collagen membranes were prepared by coating their solution on peteri dishes. Various types of cells were cultured on these membranes after being irradiated by ultraviolet ray. The denatured collagen was proved a good substratum for culturing anchorage dependent cells. A denatured collagen (gelatin) microcarrier, GT-2 was obtained by cross-linking gelatin with glutaraldehyde in a suspension polymerization process. These microcarriers were used successfully to culture various anchorage-dependent cells such as Vero, CHO, Bowes and fish cells in varying scales, including T-flasks, spinning bottles, revolving bottles and 1.5 l and 20 l bioreactors.
Chen
Y
Y
Research Institute of Biochemical Engineering, East China University of Chemical Technology, Shanghai.
Li
Y
Y
Zhang
S
S
Song
Y
Y
Ye
N
N
Hua
P
P
Yuan
T
T
Ding
J
J
D000818:Animals; D000831:Animals, Newborn; D001709:Biotechnology; D002417:Cattle; D002448:Cell Adhesion; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D003584:Cytological Techniques; D005069:Evaluation Studies as Topic; D008567:Membranes, Artificial; D012867:Skin
D008567:Membranes, Artificial; D003094:Collagen