[Functional evaluation of possible cytokine receptors on airway smooth muscle: modification of airway smooth muscle responses by cytokines].
Recently, the role of activated T-cell derived cytokines in the pathophysiology of asthma has been recognized. In this study, we evaluated the effects of interleukin-2 (IL-2) and gamma interferon (IFN-gamma) on contractile and relaxing responses of guinea-pig tracheal strips in isometric tension measurement system. IL-2 enhanced carbachol (Carb)- and KCl-induced contraction, and attenuated isoproterenol (Iso)-induced relaxation. IFN-gamma had little effect on Carb and KCl contraction, but enhanced Iso relaxation. These effects of cytokines were abolished by denuding epithelium from tracheal strips. These results suggest that IL-2 and IFN-gamma are able to affect functions of non-immune cells in the airway, and that the airway epithelium has some role in the effects of cytokines on airway responses.
Munakata
M
M
First Department of Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.
Hang
C
C
Masaki
Y
Y
Ukita
H
H
Kawakami
Y
Y
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000502:physiology*; D007376:Interleukin-2 / Q000502:physiology*; D007545:Isoproterenol / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000378:metabolism* / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology; D017471:Receptors, Interferon / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D014132:Trachea / Q000187:drug effects / Q000378:metabolism
D007376:Interleukin-2; D017471:Receptors, Interferon; D015375:Receptors, Interleukin-2; D011189:Potassium Chloride; D007371:Interferon-gamma; D002217:Carbachol; D007545:Isoproterenol