title: Nucleotide sequence of the Staphylococcus aureus gyrB-gyrA locus encoding the DNA gyrase A and B proteins.
abstract: We have determined the nucleotide sequence of a 5.3-kb segment of the Staphylococcus aureus chromosome that includes the gyrA and gyrB genes coding for both subunits of DNA gyrase, the enzyme that catalyzes ATP-dependent DNA supercoiling. The gene order at this locus, dnaA-dnaN-recF-gyrB-gyrA, is similar to that found in the Bacillus subtilis replication origin region. S. aureus recF, gyrB, and gyrA genes are closely spaced, occupy the same reading frame, and may be coordinately expressed. The S. aureus gyrB and gyrA genes encode 640- and 889-residue proteins, respectively, that share strong homology with other bacterial gyrase subunits, notably those from B. subtilis. These results are discussed in regard to the mechanism of DNA gyrase and its role as a target for the 4-quinolones and other antistaphylococcal agents.
authors.affiliation: Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, Cranmer Terrace, London, United Kingdom.
authors.forename: E E
authors.initials: EE
authors.lastname: Margerrison
authors.forename: R
authors.initials: R
authors.lastname: Hopewell
authors.forename: L M
authors.initials: LM
authors.lastname: Fisher
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D027081:DNA Gyrase; D004261:DNA Replication / Q000235:genetics*; D004250:DNA Topoisomerases, Type II / Q000187:drug effects / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009675:Novobiocin / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D046911:Macromolecular Substances; D011804:Quinolines; D009675:Novobiocin; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II