Cytogenetic adaptive response of cultured fish cells to low doses of X-rays.
The adaptive response was examining chromosomal aberrations and micronucleus in cultured fish cells, ULF-23 (mudminnow) and CAF-31 (gold fish). When cultured fish cells were first irradiated with small doses of X-rays, they became less sensitive to subsequent exposures to high doses. The effective adaptive dose was 4.8 cGy-9.5 cGy. Adaptive doses given cells in the G1 phase were more effective than when given in the S phase. The adaptive response was maximal at 5 hours and disappeared at 10 hours after the adaptive dose. The expression of the response was inhibited by treatment with 3-aminobenzamide, as reported for mammalian cells, and with arabinofuranoside cytosine, an inhibitor of DNA polymerase alpha. Caffeine, an inhibitor of post-replicational repair, had no effect on the response.
Kurihara
Y
Y
Division of Biology, National Institute of Radiological Sciences, Chiba, Japan.
Rienkjkarn
M
M
Etoh
H
H
D000222:Adaptation, Physiological / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured / Q000528:radiation effects; D003530:Cyprinidae / Q000235:genetics / Q000502:physiology*; D066298:In Vitro Techniques; D011831:Radiation Genetics