The role of calcium, polyamines and centrosomes in the formation and organization of cleavage furrows in amphibian eggs.
Cleavage furrows of amphibian eggs exhibit characteristic morphological features: the presence of finger-like microvilli (MV) along their outer edges, the formation of furrow walls from new plasma membrane lacking MV, and the subsequent retrieval of this membrane during the infolding of the furrow. A similar structure can be induced, specifically, by certain cytoplasmic components such as centrosomes, polyamines and calcium. Their respective roles in the events associated with the furrowing process have been investigated by injecting these agents into nucleated and enucleated Pleurodeles eggs and evaluating their effects using cytochemical labelling of the egg surface with a biotin-streptavidin system. The injection of polyamines (spermine or spermidine) and in some cases, calcium into enucleated eggs provoked MV elongation and the appearance of newly formed, smooth plasma membrane. In these eggs, this membrane was not incorporated into the furrows, and as a consequence, the blastomeres did not actually separate. In contrast, the injection of centrosomes into enucleated eggs induced both the incorporation and internalization of new membrane, resulting in the formation of furrows and a true cellularization of the eggs, identical to the cleavage process observed in fertilized eggs. The present results provide further evidence that the establishment of the furrow depends on two complementary interacting systems: the contractile elements of the egg cortex which regulate the insertion of new membrane and the mitotic center which is essential for the invagination of the furrow.
Aimar
C
C
Laboratoire d'Immunologie Comparée, Université Pierre et Marie Curie, CNRS URA 1135, Paris, France.
Grant
N
N
D000818:Animals; D015317:Biogenic Polyamines / Q000502:physiology*; D001710:Biotin / Q000737:chemistry; D002118:Calcium / Q000502:physiology*; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000502:physiology; D002970:Cleavage Stage, Ovum / Q000648:ultrastructure*; D004527:Egg Proteins / Q000737:chemistry; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure; D010999:Pleurodeles; D015053:Zygote / Q000648:ultrastructure
D015317:Biogenic Polyamines; D004527:Egg Proteins; D001710:Biotin; D002118:Calcium