title: Trypanosoma cruzi: incorporation of macromolecule precursors during the morphogenic process.
abstract: During the morphogenic process from epimastigote to metacyclic trypomastigote stage changes at molecular levels are produced. In the present work the incorporation of macromolecule precursors L [3H]-leucine, [3H]-thymidine and [3H]-uridine was used to monitor the differences between parasites growing with and without specific stimulation for morphogenesis (SEpi and NEpi, respectively). The peak of maximum [3H]-uridine incorporation was earlier in the SEpi than in NEpi (3 vs 4 days, respectively) reaching 129% higher values. Even at day 2, SEpi already showed values of [3H]-uridine incorporation slightly higher (29%) than the maximum reached by NEpi. One the other hand the peak of L-[3H]-leucine incorporation was 48 h delayed comparing SEpi vs NEpi with quantitatively similar patterns, while no differences were registered for [3H]-thymidine incorporation. Therefore the [3H]-uridine incorporation may constitute an earlier marker of morphogenesis since the process was optically detected starting day at 6.
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
authors.forename: E M
authors.initials: EM
authors.lastname: Lammel
authors.forename: D
authors.initials: D
authors.lastname: Turyn
authors.forename: E L
authors.initials: EL
authors.lastname: Isola
mesh_terms: D000818:Animals; D007422:Intestines / Q000737:chemistry; D007930:Leucine / Q000378:metabolism*; D009024:Morphogenesis / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology; D014224:Triatoma / Q000737:chemistry; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism*
chemical_list: D014020:Tissue Extracts; D007930:Leucine; D013936:Thymidine; D014529:Uridine