title: Eosinophil IgE receptor and CD23.
abstract: In the present review, eosinophil Fc epsilon RII was compared to CD23, a differentiation marker of B cells. Biochemical analysis revealed that molecules of similar molecular weight were immunoprecipitated from eosinophils and B cells by an anti-CD23 monoclonal antibody (mAb) or by BB10, and anti-eosinophil Fc epsilon RII. By flow cytometry, a correlation was found between the binding of anti-CD23 mAb and myeloma IgE. However, a low expression of different epitopes of CD23 was observed in various hypereosinophilic patients. Northern blot analysis of eosinophil RNA with the cDNA probe of CD23 revealed a weak message in only 3 of the 6 patients expressing membrane CD23. The inhibition by anti-CD23 mAbs of IgE-mediated cytotoxicity and IgE binding to eosinophils clearly indicated the participation of CD23 or a related molecule in IgE-dependent eosinophil functions. However, the differential effects of anti-CD23 mAbs on eosinophils and B cells suggest major differences in the characteristics of the molecule expressed by eosinophils and by B cells.
authors.affiliation: Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167-CNRS 624, Institut Pasteur, Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Capron
authors.forename: M J
authors.initials: MJ
authors.lastname: Truong
authors.forename: D
authors.initials: D
authors.lastname: Aldebert
authors.forename: V
authors.initials: V
authors.lastname: Gruart
authors.forename: M
authors.initials: M
authors.lastname: Suemura
authors.forename: G
authors.initials: G
authors.lastname: Delespesse
authors.forename: B
authors.initials: B
authors.lastname: Tourvieille
authors.forename: A
authors.initials: A
authors.lastname: Capron
mesh_terms: D000911:Antibodies, Monoclonal; D003602:Cytotoxicity, Immunologic; D004804:Eosinophils / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D017455:Receptors, IgE / Q000737:chemistry* / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D017455:Receptors, IgE