In vitro keratin expression of hair cells.
Human hair follicles were isolated from the scalp by dispase and collagenase treatment and dispersed into a cell suspension by trypsin. These cells proliferated well and could be subcultured 7 to 8 times. The medium used was MCDB 153 HAA medium further supplemented with some amino acids, hydrocortisone, insulin, EGF, and bovine brain extract. The concentration of Ca++ was adjusted to 0.1 mM. Immunohistochemically, these cells were proved to possess keratins specific to hair forming cells.
Kitano
Y
Y
Department of Dermatology, Hyogo College of Medicine, Japan.
Saito
K
K
Okamoto
E
E
Okano
Y
Y
Tanigaki-Obana
N
N
Ito
K
K
Tazawa
T
T
Ito
M
M
D046508:Culture Techniques; D006197:Hair / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis*
D007633:Keratins