Schistosoma mansoni: characterization of phosphoinositide response.
Signal transduction pathways may have important regulatory roles in cellular events in the human parasite Schistosoma mansoni. The presence of the phosphoinositide response in S. mansoni was examined by radiolabeling intact worms with 20 muCi of [3H]myoinositol for 24 hr and stimulating parasites with 25 mM NaF and 10 microM AlCl3 in the presence of 10 mM LiCl. Total inositol phosphates were increased within 2 min and maximal accumulation was achieved after 30 min. Similar results were seen with the non-hydrolyzable GTP analogues GTP gamma S and GppNHp while only minimal changes were detected with GMP. Neomycin inhibited NaF-induced inositol phosphate production. NaF stimulated a significant 3.6-fold increase of inositol phosphates in females compared to males. These data suggest that stimulation of guanine nucleotide-binding regulatory proteins activates phospholipase C resulting in production of inositol phosphates in S. mansoni.
Wiest
P M
PM
Department of Medicine, Miriam Hospital, Providence, Rhode Island 02906.
Li
Y N
YN
Burnham
D C
DC
Olds
G R
GR
Bowen
W D
WD
D000535:Aluminum / Q000494:pharmacology; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D002712:Chlorides / Q000494:pharmacology; D005260:Female; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D008297:Male; D009355:Neomycin / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D012550:Schistosoma mansoni / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D012969:Sodium Fluoride / Q000494:pharmacology
D017607:Aluminum Compounds; D002712:Chlorides; D010716:Phosphatidylinositols; D000077410:Aluminum Chloride; D006160:Guanosine Triphosphate; D012969:Sodium Fluoride; D008094:Lithium; D000535:Aluminum; D018021:Lithium Chloride; D009355:Neomycin