title: Hemoglobin repletion in anemic rats fed a new bean cultivar (Phaseolus vulgaris L.) as source of iron.
abstract: Utilization of iron from a new cultivar of dry bean (Carioca 80 SH) was estimated using anemic rat by two methods: the slope ratio and the hemoglobin regeneration methods. The bean was incorporated into the diet in the form of whole bean flour (WBF) 7.45 mg Fe/100 g or as partially decorticated bean flour (DBF), 8.57 mg Fe/100 g. Hemoglobin concentration in the rats' blood at the beginning of the experiment averaged 13.79 +/- 0.33 g Hb/dl; at the end of the depletion period it ranged from 5.62 +/- 0.38 to 6.30 +/- 0.39 g Hb/dl blood, difference which was not statistically significant (p < or = 0.05). The bean iron relative biological value (RBV, relative to FeSO4 = 100) evaluated by the slope ratio method, was 74% for the WBF and 77% for the DBF. The hemoglobin regeneration efficiency was 60.4 +/- 6.5, 50.8 +/- 3.2, and 52.7 +/- 5.7%, respectively, for the iron sulfate (reference source), the WBF, and the DBF.
authors.affiliation: Departamento de Planejamento Alimentar e Nutrição, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas, São Paulo, Brasil.
authors.forename: M M
authors.initials: MM
authors.lastname: Cabral
authors.forename: V C
authors.initials: VC
authors.lastname: Sgarbieri
mesh_terms: D000740:Anemia / Q000097:blood*; D000818:Animals; D004032:Diet; D007887:Fabaceae / Q000737:chemistry / Q000378:metabolism*; D006454:Hemoglobins / Q000032:analysis / Q000502:physiology*; D007501:Iron / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D009753:Nutritive Value; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006454:Hemoglobins; D007501:Iron