Use of an ammonia electrode for rapid quantification of Helicobacter pylori urease: its use in the endoscopy room and in the assessment of urease inhibition by bismuth subsalicylate.
The use of an ammonia electrode to quantify ammonia liberated by urease from Helicobacter pylori was assessed in an in vitro study. It was found to be highly sensitive (down to 0.7 ppm NH3) and highly reproducible (coefficient of variation 6.0%). Inhibition of urease by bismuth subsalicylate was evaluated as urease testing is often used to assess clearance of H. pylori in patients treated with bismuth. Concentrations of bismuth subsalicylate up to 5 mg/ml had no inhibitory effect but bismuth subsalicylate at 50 mg/ml resulted in 21% inhibition of the urease activity of an ultrasonicated H. pylori suspension. As a preliminary study, the ammonia electrode was assessed in the endoscopy room in comparison with conventional techniques for H. pylori diagnosis. Antral biopsies from 39 patients attending for routine diagnostic endoscopy were subjected to culture, histology, detection of urease activity with a commercially available slide test (CLO) and with the ammonia electrode to detect ammonia liberated from samples placed in urea solution. 21 patients were positive after 1 h with the ammonia electrode, compared to only 17 with the commercially available slide test. 20 were positive on histology and 19 by culture. All samples positive with the ammonia electrode were either positive by culture or by histology. The ammonia electrode offers a quick, sensitive, quantitative and cheap method for the detection and quantification of H. pylori.
Butcher
G P
GP
University Department of Medicine, Walton Hospital, Liverpool, UK.
Ryder
S D
SD
Hughes
S J
SJ
Stewart
M
M
Bird
N
N
Haqqani
M T
MT
Rhodes
J M
JM
D000641:Ammonia; D001729:Bismuth / Q000494:pharmacology*; D004566:Electrodes; D005260:Female; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000188:drug therapy; D016480:Helicobacter pylori / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009942:Organometallic Compounds / Q000494:pharmacology*; D012459:Salicylates / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D014510:Urease / Q000032:analysis* / Q000037:antagonists & inhibitors
D009942:Organometallic Compounds; D012459:Salicylates; C015715:bismuth subsalicylate; D000641:Ammonia; D014510:Urease; D001729:Bismuth