title: Calcium accumulation in synapses of the rat hippocampus after cerebral ischemia.
abstract: The ultrastructural localization of calcium deposits in the synapses of rat hippocampus after 10 min global cerebral ischemia was evaluated. Oxalate-pyroantimonate technique was applied. After 24 hours of postischemic recirculation enhancement of intracellular (pre- and postsynaptic parts) and extracellular (synaptic clefts) calcium deposits was found in great proportion of synapses in CA1 sector. Abundant Ca-precipitates appeared specially in synaptic clefts and in the postsynaptic parts near synaptic densities. Increased calcium deposits in some changed mitochondria were also observed. The results presented in this paper suggest synaptic modulation of Ca2+ homeostasis, disturbed after ischemic incident. Presence of Ca-precipitates in synaptic clefts and postsynaptic parts seems to be a sensitive indicator of increased calcium influx from the extracellular to the intracellular compartments.
authors.affiliation: Department of Neuropathology, Polish Academy of Sciences, Warsaw.
authors.forename: B
authors.initials: B
authors.lastname: Gajkowska
authors.forename: M J
authors.initials: MJ
authors.lastname: Mossakowski
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D002114:Calcinosis / Q000378:metabolism*; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D002118:Calcium