title: The multifunctional folic acid synthesis fas gene of Pneumocystis carinii appears to encode dihydropteroate synthase and hydroxymethyldihydropterin pyrophosphokinase.
abstract: We describe the cloning of a multifunctional folic acid synthesis (fas) gene from Pneumocystis carinii. The nucleotide sequence contains an open reading frame interrupted by three introns, that encodes a protein of 740 amino acids with an Mr of 97,278. The predicted Fas protein has homology to two enzyme domains, dihydropteroate synthase and 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, both of which are involved in folate synthesis, and at least one other region of unknown function.
authors.affiliation: Department of Molecular Sciences, Wellcome Research Laboratories, Kent, U.K.
authors.forename: F
authors.initials: F
authors.lastname: Volpe
authors.forename: M
authors.initials: M
authors.lastname: Dyer
authors.forename: J G
authors.initials: JG
authors.lastname: Scaife
authors.forename: G
authors.initials: G
authors.lastname: Darby
authors.forename: D K
authors.initials: DK
authors.lastname: Stammers
authors.forename: C J
authors.initials: CJ
authors.lastname: Delves
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004094:Dihydropteroate Synthase / Q000235:genetics*; D017854:Diphosphotransferases; D005492:Folic Acid / Q000096:biosynthesis*; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000235:genetics*; D011010:Pneumocystis / Q000201:enzymology* / Q000235:genetics
chemical_list: D005492:Folic Acid; D004094:Dihydropteroate Synthase; D010770:Phosphotransferases; D017854:Diphosphotransferases; C019684:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase