The determination of the geometric isomers and related impurities of dothiepin in a pharmaceutical preparation by capillary electrophoresis.
The application of capillary zone electrophoresis in the assay of the tricyclic antidepressant drug, dothiepin, in tablets is discussed. The method developed for dothiepin which exists as the cis- and trans-isomers and contains two major related impurities, an 11-oxo compound and a propanamine, utilizes inclusion complexation with beta-cyclodextrin. For optimization of the method the structured procedure of factorial design was used; the electrolyte solution was 50 mM disodium hydrogen phosphate with 10 mM beta-cyclodextrin-propan-1-ol(90:10, v/v). Good precision (RSD = 1.06%, n = 6), linearity (y = 26.84x + 2.25), and correlation (r = 0.999, n = 7) was obtained for trans-dothiepin. The reproducibility of tablet extraction was also acceptable (RSD = 0.77%, n = 6); the recovery of the trans-isomer was 98% (w/w) and the level of cis-isomer in tablets of dothiepin (75 mg) was 5.58% (w/w).
Clark
B J
BJ
Pharmaceutical Chemistry, School of Pharmacy, University of Bradford, UK.
Barker
P
P
Large
T
T
D003505:Cyclodextrins; D004308:Dothiepin / Q000032:analysis* / Q000737:chemistry; D004340:Drug Contamination; D004586:Electrophoresis; D013237:Stereoisomerism; D013607:Tablets
D003505:Cyclodextrins; D013607:Tablets; D004308:Dothiepin