Pristane induced gene activation.
Studies were performed to examine the effects of 2,6,10,14-tetramethyl pentadecane (pristane) versus 12-O-tetradecanoylphorbol 13-acetate (TPA) on the activation of the CAT gene under the regulatory control of viral promoter/enhancer elements transfected into NIH-3T3, CV-1 and COS-7 cells. The results of these studies demonstrated that (1) pristane or TPA induced trans-activation of SV2cat, HIVcat, RSVcat and MMTVcat in cells transfected with each respective plasmid construct, (2) only pristane induced activation of pA10cat and pOSP/11 and (3) neither TPA nor pristane trans-activated pSV0cat. Furthermore, treatment with either pristane or TPA elicited changes in the morphology of each of the cell lines. Collectively these results indicate that pristane is a potent inducer of gene expression and exhibits similar characteristics as the tumor promoter, TPA.
Garrett
L R
LR
Department of Microbiology University of Mississippi Medical Center, Jackson 39216-4505.
Jones
C J
CJ
Cuchens
M A
MA
D016475:3T3 Cells; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D002461:Cell Line, Transformed; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005798:Genes, Bacterial; D051379:Mice; D010957:Plasmids; D013539:Simian virus 40; D013729:Terpenes / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015533:Transcriptional Activation; D014162:Transfection
D002273:Carcinogens; D013729:Terpenes; C009042:pristane; D015500:Chloramphenicol O-Acetyltransferase; D013755:Tetradecanoylphorbol Acetate