Role of toxic ingredients in silicone oils in the induction of increased corneal endothelial permeability.
Silicone oils may induce pathological changes in corneas or retinas by unknown mechanisms but the effects are probably related to certain specific components. Low molecular weight compounds have been implicated in the induction of toxic tissue reactions. Several of these components, that occur as contaminants or by-products in crude silicone oils, were tested for their ability to alter corneal endothelial permeability. In vitro inulin/dextran permeability was measured after one week of in vivo exposure to a non-toxic oil to which various low molecular weight components were added. At least 75% of the anterior chamber volume was replaced with oil +/- additives. A long-chain silanol-terminated polydimethylsiloxane (1000 cps) at 2 mg/ml, tetramethyl-ammonium siloxanolate (a catalyst) at 1 mg/ml and a mixture of a series of linear compounds (MM through MD10M) each at 10 mg/ml all caused a large corneal endothelial permeability increase. A mixture of two short-chain silanol-terminated compounds was less damaging, as was a mixture of a cyclic series. Evidently certain compounds can induce toxic effects on the corneal endothelium whereas other compounds are much less toxic. The linear series and the catalyst, that induce corneal endothelial changes, have been shown to occur in silicone oils.
Green
K
K
Department of Ophthalmology, Medical College of Georgia, Augusta 30912-3400.
Cheeks
L
L
Stewart
D A
DA
Trask
D
D
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003228:Conjunctiva / Q000098:blood supply; D015715:Corneal Edema / Q000139:chemically induced; D003911:Dextrans / Q000378:metabolism; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000378:metabolism; D006940:Hyperemia / Q000139:chemically induced; D007444:Inulin / Q000378:metabolism; D011817:Rabbits; D012827:Silicone Oils / Q000633:toxicity*
D003911:Dextrans; D012827:Silicone Oils; D007444:Inulin