title: [Preparation of antigen and antibody against aflatoxin B1].
abstract: Carboxymethyloxime of aflatoxin B1 (AFB1) was synthesized by refluxing AF B1 with carboxymethoxylamine hemihydrochloride for 4 hours in the presence of pyridine. AF B1-BSA (antigen) was made successfully by coupling AF B1-oxime with bovine serum albumin (BSA) in the presence of a water soluble carbodiimide. The rabbits were immunized with the artificial immunogen which had been purified by dialysis. The titers of antisera were determined by indirect enzyme-linked immunosorbent assay (ELISA) which was developed by ourselves. The highest titer was 1:100,000.
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: C
authors.initials: C
authors.lastname: Lu
authors.forename: Z
authors.initials: Z
authors.lastname: Xiao
authors.forename: G
authors.initials: G
authors.lastname: Wang
mesh_terms: D016604:Aflatoxin B1 / Q000276:immunology*; D000418:Albumins / Q000276:immunology; D000818:Animals; D000941:Antigens / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000032:analysis*; D011817:Rabbits
chemical_list: D000418:Albumins; D000941:Antigens; D007106:Immune Sera; D016604:Aflatoxin B1