An acidic region of the 89K murine cytomegalovirus immediate early protein interacts with DNA.
The product of the ie1 gene, the regulatory immediate early protein pp89 of murine cytomegalovirus (MCMV), interacts with core histones, which can mediate the association of pp89 with DNA. We report the capacity of pp89 to interact directly with DNA in the absence of cellular proteins. After separation of proteins by SDS-PAGE, pp89 bound ds- and ssDNA, with a preference for ssDNA. Binding to specific DNA sequences in the MCMV genome was not detected. The DNA-binding region of pp89 was located to amino acids 438 to 534 by analysis of deletion mutants expressed as beta-galactosidase or TrpE fusion proteins. This region is identical to the highly acidic C-terminal region spanning amino acids 424 to 532. The human cytomegalovirus IE1 protein, which contains a similar extended C-terminal acidic region, does not react with DNA under the same experimental conditions.
Münch
K
K
Abteilung Virologie, Universität Ulm, Germany.
Messerle
M
M
Plachter
B
B
Koszinowski
U H
UH
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics*; D004252:DNA Mutational Analysis; D004277:DNA, Single-Stranded / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons / Q000235:genetics; D006657:Histones / Q000737:chemistry; D017874:Immediate-Early Proteins; D051379:Mice; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D006657:Histones; D017874:Immediate-Early Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C052612:cytomegalovirus immediate early phosphoprotein pp89