Competition of Mn2+ and Zn2+ with 59Fe2+ and 59Fe3+ for the plasma membrane receptors from lactating mouse mammary gland.
Nonlabeled MnCl2 and ZnSO4 compete with 59Fe(2+)-ascorbate and 59Fe2(3+)O3 for transport binding sites situated on the plasma membranes of lactating mouse mammary gland cells. The binding was found to be a process reaching saturation. The heterologous competition used here ruled out the participation of transferrin and to propose that Fe, Mn, and Zn are transported from blood to milk by a mechanism involving one receptor during lactation. Further experiments are necessary to establish the details of the transport mechanism.
Moutafchiev
D A
DA
Department of Biochemistry, Research Medico-Biological Institute, Medical Academy, Sofia, Bulgaria.
Sirakov
L M
LM
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides; D005260:Female; D005290:Ferric Compounds / Q000378:metabolism*; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism*; D017895:Manganese Compounds; D051379:Mice; D013431:Sulfates / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*; D019287:Zinc Sulfate
D002712:Chlorides; D005290:Ferric Compounds; D017895:Manganese Compounds; D013431:Sulfates; C000499:ferric oxide; D008345:Manganese; D019287:Zinc Sulfate; D015032:Zinc; D001205:Ascorbic Acid; C025340:manganese chloride