title: Separation and characterization of diastereoisomeric oligonucleotide.
abstract: For developing the antisense method, two types of oligonucleotides suitable for antisense molecules were explored for their essential properties. One is oligonucleoside phosphorothioate, and all their possible R/S diastereoisomers were separated and purified by reversed phase liquid chromatography. Isolated diastereoisomers have been investigated for the correlation between their configuration and hybridization manner. Separation, R/S characterization and spectroscopical properties of these oligonucleotides will be discussed.
authors.affiliation: Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Murakami
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
authors.forename: M
authors.initials: M
authors.lastname: Nishi
authors.forename: T
authors.initials: T
authors.lastname: Morii
authors.forename: H
authors.initials: H
authors.lastname: Ide
authors.forename: K
authors.initials: K
authors.lastname: Makino
mesh_terms: D002851:Chromatography, High Pressure Liquid; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000302:isolation & purification; D009943:Organophosphorus Compounds / Q000737:chemistry; D013237:Stereoisomerism; D013873:Thionucleotides / Q000737:chemistry
chemical_list: D016376:Oligonucleotides, Antisense; D009943:Organophosphorus Compounds; D013873:Thionucleotides