The simian varicella virus and varicella zoster virus genomes are similar in size and structure.
Simian varicella virus (SVV) DNA was purified from viral nucleocapsids and the molecular structure of the SVV genome was determined. SVV DNA was analyzed by agarose gel electrophoresis of BamHI, BglII, EcoRI, and PstI restriction endonuclease digests. SVV and varicella zoster virus (VZV) DNAs were demonstrated to have distinct restriction endonuclease profiles. Summation of the sizes of individual restriction endonuclease fragments indicate the size of SVV DNA is congruent to 121 kilobase pairs (kbp) or congruent to 76.8 megadaltons (Md). Electron microscopy, lambda exonuclease analysis, and Southern blot DNA hybridizations were utilized to determine the molecular structure of the SVV genome and to construct restriction endonuclease maps. The results indicate that SVV DNA consists of a long component (L, congruent to 100 kbp) covalently linked to a short component (S, congruent to 20 kbp) which is composed of a unique short sequence (Us, 5.3 +/- 0.7 kbp) bracketed by inverted repeat sequences (TRs and IRs, congruent to 7.2 kbp). The presence of 0.5 M PstI restriction endonuclease fragments indicates that the S component may invert relative to the L component and that the genome exists in two major isomeric forms. The findings demonstrate that the SVV and VZV genomes are similar in size and structure.
Gray
W L
WL
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205.
Pumphrey
C Y
CY
Ruyechan
W T
WT
Fletcher
T M
TM
D000818:Animals; D015139:Blotting, Southern; D004279:DNA, Viral / Q000648:ultrastructure; D016679:Genome, Viral; D000882:Haplorhini / Q000382:microbiology; D006564:Herpesviridae / Q000235:genetics*; D014645:Herpesvirus 3, Human / Q000235:genetics*; D008854:Microscopy, Electron; D015183:Restriction Mapping; D014709:Vero Cells
D004279:DNA, Viral