Preparation of human chromosomes for high resolution scanning electron microscopy.
The addition of ethidium bromide during the last 2.5-3 h of lymphocyte culturing restricted chromosome contraction and preserved the banding structure in scanning electron microscopy. Treatment of the chromosomes with trypsin and use of impregnation with osmium tetroxide and thiocarbohydrazide resulted in a structural preservation of high resolution quality.
Kättström
P O
PO
Department of Human Anatomy, Uppsala University, Sweden.
Nilsson
B O
BO
D002478:Cells, Cultured; D002875:Chromosomes / Q000648:ultrastructure*; D004996:Ethidium; D006801:Humans; D006834:Hydrazines; D008214:Lymphocytes / Q000166:cytology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009993:Osmium Tetroxide; D014357:Trypsin
D006834:Hydrazines; C011368:thiocarbohydrazide; D014357:Trypsin; D004996:Ethidium; D009993:Osmium Tetroxide