Protection of isolated perfused working rat heart from oxidative stress by exogenous L-propionyl carnitine.
The effect of exogenous L-propionyl carnitine on peroxidative injury was investigated on isolated working rat hearts. The addition of 190 microM hydrogen peroxide to the perfusion buffer caused a marked decrease in aortic flow, minute work and peak aortic pressure, and a release of intracellular enzymes. In the presence of L-propionyl carnitine the haemodynamic damage was significantly lower and enzyme leakage remarkably decreased. The protection was concentration-dependent and the whole structure of the molecule was required, since carnitine alone was found less effective and propionate had no effect. In the absence of hydrogen peroxide L-propionyl carnitine increased heart performance. The effect of L-propionyl carnitine on oxidative stress could account for the beneficial effect of this substance in different models of ischaemic injury. L-propionyl carnitine increases the cardiac performance and protects the rat heart from peroxidation through metabolic and antiperoxidative mechanisms.
Ronca
G
G
Institute of Biological Chemistry, University of Pisa, Italy.
Ronca
F
F
Yu
G
G
Zucchi
R
R
Bertelli
A
A
D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D002331:Carnitine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D004347:Drug Interactions; D006321:Heart / Q000187:drug effects*; D006439:Hemodynamics / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
D002316:Cardiotonic Agents; C003223:propionylcarnitine; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D002331:Carnitine