title: Albendazole and mebendazole uptake by isolated enterocytes.
abstract: Uptake of albendazole (ABZ) and mebendazole (MBZ) by isolated rat enterocytes was carried out. These drugs, widely used oral anthelmintics, exhibit a scarce water solubility which reduce its absorption by the oral tract. The present study was designed to assess the captation for ABZ and MBZ in different enterocyte populations isolated from upper to crypt villus. The concentration range used for the absorption experiments was within 10-500 microM for both drugs, using DMSO as solvent. The results obtained show the existence of a passive mechanism for the uptake of ABZ and MBZ at concentrations between 10 and 100 microM, with a maximum intake value around 20 microM/mg protein. No differences were found with respect to the cell populations analyzed. The drug uptake levels seem to be higher for MBZ than for ABZ prior to reaching the maximum plateau.
authors.affiliation: Department of Physiology, Pharmacology and Toxicology, University of Leon, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Villaverde
authors.forename: A I
authors.initials: AI
authors.lastname: Alvarez
authors.forename: J L
authors.initials: JL
authors.lastname: del Estal
authors.forename: J G
authors.initials: JG
authors.lastname: Prieto
mesh_terms: D015766:Albendazole / Q000378:metabolism* / Q000493:pharmacokinetics; D000818:Animals; D004386:Duodenum / Q000166:cytology / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D008463:Mebendazole / Q000378:metabolism* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008463:Mebendazole; D015766:Albendazole