title: Identification of "B" receptor for natriuretic peptide in human kidney.
abstract: Three distinct receptor types for natriuretic peptides (NP) have been identified in human tissue. "A" and "B" receptors initiate biological actions, whereas the "C" receptor has a clearance function. It has been proposed that the natural ligand for the B receptor is c-type natriuretic peptide (CNP), rather than atrial natriuretic peptide (ANP), and that the B receptor is only found in the central nervous system (CNS) and is responsible for all NP-mediated effects in the CNS. Contrary to this hypothesis, we have identified, by means of the polymerase chain reaction (PCR), the B receptor in human kidney tissue. To detect A and C receptors, the PCR reaction was performed with primers which yielded predicted 600 and 378 base pair (bp) products, respectively. For the B receptor, 3 different primer sets were used, resulting in the expected 785, 453 and 228 bp fragments. Restriction mapping of the latter two products with Rsa I yielded the expected fragment numbers and sizes, indicating the PCR products were from B receptor mRNA. These results indicate that the human kidney has B as well as A and C receptors. Thus CNP may have a renal as well as a CNS site of action.
authors.affiliation: Division of Nephrology, Stanford University School of Medicine, CA 94305.
authors.forename: S
authors.initials: S
authors.lastname: Canaan-Kühl
authors.forename: R L
authors.initials: RL
authors.lastname: Jamison
authors.forename: B D
authors.initials: BD
authors.lastname: Myers
authors.forename: R E
authors.initials: RE
authors.lastname: Pratt
mesh_terms: D001483:Base Sequence; D006801:Humans; D007668:Kidney / Q000737:chemistry*; D008969:Molecular Sequence Data; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics
chemical_list: D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C058930:brain natriuretic peptide receptor; D020097:Natriuretic Peptide, Brain; D017461:Receptors, Atrial Natriuretic Factor