Differential regulation of the rat melatonin receptors: selective age-associated decline and lack of melatonin-induced changes.
To gain some understanding of the factors regulating high affinity melatonin (MT) receptors in the rat, we conducted a series of studies using quantitative autoradiography of [2-125I]iodo-MT binding in vitro with validated assay conditions. MT receptor status and the relative protein content of the autoradiographic sections were assessed in the anterior pituitary gland, the area postrema, the caudal (tail) artery (CA), the anterior cerebral artery (ACA), and the suprachiasmatic nuclei (SCN) of 9-, 96-, and 306-day-old Wistar rats. When age-associated changes in protein content were taken into account, MT receptor expression in the area postrema and the SCN remained relatively constant between 9-306 days of age. On the contrary, a dramatic loss of MT receptors was observed in the arteries of 306-day-old rats (98% and 89% loss compared to the 9-day-old rats in the ACA and CA, respectively). In the anterior pituitary gland, MT receptors were expressed only in the 9-day-old rats. The above changes reflected major changes in binding capacity and minor changes in binding affinity. Neither removal of endogenous circulating MT (acute light exposure for 24 h, pinealectomy, or superior cervical ganglionectomy) nor MT injections (1 mg/kg for 10 days 6 h after lights on) affected MT receptor status in the ACA, CA, area postrema, or SCN. Our data suggest that MT receptor expression is differentially regulated during development and that permanent alterations in MT levels do not affect rat MT receptor status.
Laitinen
J T
JT
Section of Pharmacology, National Institute of Mental Health, Bethesda, Maryland 20892.
Viswanathan
M
M
Vakkuri
O
O
Saavedra
J M
JM
D000367:Age Factors; D000818:Animals; D001158:Arteries / Q000737:chemistry; D001923:Brain Chemistry; D008297:Male; D008550:Melatonin / Q000378:metabolism / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D044094:Receptors, Melatonin; D017981:Receptors, Neurotransmitter / Q000032:analysis*
D044094:Receptors, Melatonin; D017981:Receptors, Neurotransmitter; D008550:Melatonin