[The role of ultrasound technique in blood group typing].
The work presented here concerns the study and fabrication of a robot. This tool allows one to bring out the enormous potential represented by the interaction of ultrasounds with globular suspension. The traditional hemagglutination techniques employ the systematic use of a centrifuge and mechanical suspension. The advantage of these processes is the acceleration of reactions, however, the major inconvenience remains in the difficulty of automating these tests completely, especially when microplaques are used. Our study demonstrates that the force of the ultrasonic radiation created in an atmosphere of suspension is susceptible to replacing all mechanical movements. In a stationary ultrasonic field the erythrocytes in suspension assemble towards the pressure points. This phenomenon is characterized by a faster rate of sedimentation favoring agglutination as well. Under certain conditions the ultrasonic bundle produces small whirlwinds in the bowl. The result of this process is the homogenization of the suspension which permits the differentiation between the positive and negative reactions. With these observations in mind we developed a programmable robot prototype, the goal of which was to provide us with a means of greater investigation. The originality of this technique necessitated the realization of a high frequency ultrasound captor with large range and power. For supplying the necessary energy to the transducer we developed an electronic force based on the principle of commutation. The automation is controlled by a programmable microsequensor. This instrument will allow a standardization of a protocol primarily for tests of the group ABO. The results obtained are very encouraging; the next step is to further advance experimentation in more delicate tests.(ABSTRACT TRUNCATED AT 250 WORDS)
Makris
P
P
Université de Tours, Ecole d'ingénieurs en informatique pour l'industrie (E31), France.
Patat
F
F
Pourcelot
L
L
D001788:Blood Grouping and Crossmatching / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D012371:Robotics / Q000295:instrumentation / Q000379:methods; D014465:Ultrasonics
Apport de la technique ultrasonore dans la réalisation des groupages sanguins.