Standards and controls in DNA assays.
Automated methods are described that begin to standardize genetic analysis at the DNA level. These new methods complement existing automation such as DNA extraction, liquid-handling robotics, thermal cycling and computer information management systems. Using electrophoresis, several independent genetic loci can be analyzed simultaneously using multi-color fluorescent gel scanning detection where DNA segments labeled with up to four different fluorescent dyes are analyzed within a single electrophoresis lane. The amounts and molecular lengths of PCR products are determined with high accuracy and precision by co-electrophoresis with a uniquely colored internal standard. These new methods have the potential of minimizing variations in results among different laboratories.
Mayrand
P E
PE
Applied Biosystems Inc., Foster City, CA 94404.
D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005820:Genetic Testing / Q000379:methods*; D066298:In Vitro Techniques; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards
D004247:DNA