Immunocytochemical analysis of blood lymphocytes in uveitis.
We studied the surface expression of activation markers IL2-R, HLA-DR and CD45-RO on peripheral T-lymphocytes in two groups of patients (n = 26) with idiopathic uveoretinitis, compared with controls. Thirteen patients were analysed by alkaline phosphatase anti-alkaline phosphatase (APAAP) immunocytochemistry, which demonstrated a significant rise in expression of HLA-DR and IL2-R surface markers. Flow cytometric analysis was performed on a further 13 patients, which confirmed a significant rise in IL2-R expression in uveitis patients. Within this group systemic activation was confined to patients with idiopathic retinal vasculitis. Dual flow cytometry confirmed a CD4+,IL2--R+ T--lymphocyte phenotype. A further 4 patients with retinal vasculitis who had been treated with cyclosporin A demonstrated a 32% reduction in IL2-R expression over a 3-month period. Analysis of CD45-RO and CD5+ cells was found to be uninformative in this study. We have demonstrated activated peripheral lymphocytes in patients predominantly with retinal vasculitis, the significance of which is discussed.
Dick
A D
AD
Department of Ophthalmology, Medical School, Aberdeen University, Foresterhill, UK.
Cheng
Y F
YF
Purdie
A T
AT
Liversidge
J
J
Forrester
J V
JV
D015415:Biomarkers / Q000032:analysis; D016572:Cyclosporine / Q000627:therapeutic use; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D017493:Leukocyte Common Antigens / Q000032:analysis; D008213:Lymphocyte Activation / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D012171:Retinal Vessels; D012173:Retinitis / Q000188:drug therapy / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D015866:Uveitis, Posterior / Q000276:immunology*
D015415:Biomarkers; D006684:HLA-DR Antigens; D015375:Receptors, Interleukin-2; D016572:Cyclosporine; D017493:Leukocyte Common Antigens