[An electrophoretic analysis of human glycoprotein hormones and their subunits].
Stability of heterodimers of human glycoprotein hormones with gonadotropic and thyrotropic activities in sodium dodecylsulfate (SDS) under non-reducing conditions at low temperature permits to resolve the native molecules of these hormones in SDS-PAG and to distinguish from their dissociated subunits by electrophoretical mobility. The analysis of dimers and alpha-, beta-subunits in one polyacrylamide gel allows to detect certain human glycoprotein hormones and to study some of their physico-chemical properties. Using two polyclonal antisera against human LH and FSH by the Western blot immunoassay it was shown that heterodimers as well as alpha and beta subunits after SDS-PAGE retain antigenic activity of native hormones. The method gave possibility to characterize the specificity of the given sera to different glycoprotein hormones.
Govorun
M S
MS
Osipova
T A
TA
Khil'ko
S N
SN
Martynov
A V
AV
Bulatov
A A
AA
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D015292:Glycoprotein Hormones, alpha Subunit / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D015151:Immunoblotting; D007986:Luteinizing Hormone / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D011817:Rabbits
D015292:Glycoprotein Hormones, alpha Subunit; D007106:Immune Sera; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
Elektroforeticheskiĭ analiz glikoproteinovykh gormonov cheloveka i ikh sub''edinits.