Mutagenicity of analgesics, their derivatives, and anti-inflammatory drugs with S-9 mix of several animal species.
An investigation was undertaken to determine whether analgesics and their derivatives (13 compounds), and anti-inflammatory drugs (4 compounds) had mutagenicity. Rec-assay was used to clarify specific DNA-damaging properties, and the Ames test was used to find back-mutations, using S-9 fractions obtained from the liver of 4 animal species pretreated with polychlorobiphenyl. In the Rec-assay, salicylic acid (2 mg), aspirin (5 mg), benzoic acid (4 mg), sulpyrine (0.4 mg), indomethacin (0.1 mg), oxyphenbutazone (0.1 mg) and diclofenac sodium (0.1 mg) showed a DNA-damaging tendency. In the Ames test, mutagenicity of methyl salicylate was demonstrated using the Salmonella typhimurium TA98 strain upon addition of hamster S-9 mixture. Weak mutagenicity was also found using the TA100 strain with rat S-9 mixture for salicylic acid, sulpyrine, indomethacin and oxyphenbutazone, and with hamster S-9 mixture for methyl salicylate, acetaminophen and phenacetine.
Kuboyama
N
N
Department of Pharmacology, Nihon University School of Dentistry, Chiba, Japan.
Fujii
A
A
D000700:Analgesics / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D006224:Cricetinae; D004247:DNA / Q000187:drug effects*; D006168:Guinea Pigs; D008099:Liver / Q000187:drug effects; D008110:Liver Extracts / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008815:Mice, Inbred Strains; D008861:Microsomes / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012459:Salicylates / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D008110:Liver Extracts; D009153:Mutagens; D011720:Pyrazoles; D012459:Salicylates; D004247:DNA