[Effect of pollen extract (Cernitin) on the course of poisoning with organic solvents (biochemical analysis)].
The aim of the study has been to experimentally estimate the chronic exposure of selected biochemical parameters of serum and microsomal level fraction in animals to a mixture of organic solvents. An attempt was made to alleviate the eventual changes by applying Cernitin preparation. The experiment was performed on male rats, Wistar strain. The rats were exposed to the organic solvents in a toxicological chamber with controlled parameters. Cernitin preparation was added to standard diet, being given to the animals in the form of balls. The biochemical investigations were carried out after a lapse of 3 and 6 month exposition. The range of the accomplished studies included: activity of enzymes (AspAT, AlAT, AP, ChE) bilirubin level and lipids content in blood serum. Lipids content was determined in liver homogenate. The content of protein, cholesterol, phospholipids and free fatty acids, was studied in liver microsomes. It has been shown that protracted exposure to the mixture of organic solvents elicits an increase in the activity of the studied aminotransferases and alkaline++ phosphatase, as well as a decrease in the activity of cholesterase. The changes in activity are accompanied by a rise in the content of lipids. Cernitin preparation used prophylactically normalizes impairments affecting the studied enzymatic and lipid parameters.
Ceglecka
M
M
Zakładu Toksykologii Instytutu Farmakologii, Toksykologii Pomorskiej Akademii Medycznej.
D000410:Alanine Transaminase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000931:Antidotes / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000627:therapeutic use*; D002802:Cholinesterases / Q000378:metabolism; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D006838:Hydrocarbons / Q000506:poisoning*; D050356:Lipid Metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012434:Secale
D000931:Antidotes; D002800:Cholinesterase Inhibitors; D006838:Hydrocarbons; D010936:Plant Extracts; C010070:cernilton; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D002802:Cholinesterases; D000469:Alkaline Phosphatase
Wpływ wyciagów z pyłków kwiatowych (preparat Cernitin) na przebieg zatrucia rozpuszczalnikami organicznymi (analiza biochemiczna).