[Comparison of MB-Check, BACTEC, and egg-based media for recovery of mycobacteria].
The rate of recovery and time to the detection of mycobacteria from clinical specimens were measured for biphasic and radiometric liquid-based culture systems and egg-based media (3% Ogawa and Ogawa K). From the 245 sputum specimens processed, a total of 86 (35.1%) mycobacterial isolates were detected. Of these, 81 (94.2%) and 80 (93.0%) isolates were detected with the MB-Check and BACTEC systems, respectively, and 65 (75.6%) isolates were detected with the 3% Ogawa egg method. The difference in the percentages of positive cultures between the two systems based on liquid media and the 3% Ogawa egg method was significant (P < 0.01). This difference was even greater among smear-negative specimens. The detection time was shorter with the liquid-based systems. The mean times to the detection of the Mycobacterium tuberculosis complex were 19.1 days with the MB-Check system, 13.4 days with the BACTEC system, and 21.7 days with the 3% Ogawa egg method. These results indicate that both the MB-Check and the BACTEC systems, based on liquid media, are efficient for the recovery of mycobacteria.
Abe
C
C
Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo.
Hosojima
S
S
D003470:Culture Media; D004531:Eggs; D006801:Humans; D015269:Mycobacterium avium Complex / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology; D014376:Tuberculosis / Q000175:diagnosis
D003470:Culture Media