title: The distribution of centrosomes in migrating endothelial cells during wound healing in situ.
abstract: We have examined the distribution of centrioles in rabbit thoracic aortic endothelial cells induced to migrate by wounding the endothelium in situ. Following denudation of the endothelium from a segment of the aorta with a balloon catheter, a wound edge was created from which endothelial cells began to migrate onto the denuded surface. In this in situ model of cell migration, the position of centrioles was determined in cells along the wound edge by immunofluorescence and antibodies which specifically label these cell organelles, and then they were classified in relation to the nucleus and the direction of cell migration as being oriented toward the wound, in the center, or away from wound. At time 0, as in normal unwounded adult rabbit aorta, no preferential orientation of centrioles was evident. Within 12 h after wounding, the centrioles in about 53% of endothelial cells near the wound edge were oriented toward the wound, while in less than 20% of the cells they were oriented away from wound. At 24 h, in cells up to 800 microns from the wound edge, centrioles in only about 10% of the endothelial cells were oriented away from wound, while in about 52% of cells they were found in the center and in 38% of the cells they remained oriented toward the wound. At 48 h, up to 2000 microns from the wound edge, the majority of endothelial cells had their centrioles in the center, possibly as a result of an increase in mitotic index as cells replicate to reestablish an intact endothelium.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy, University of Western Ontario, London, Canada.
authors.forename: K A
authors.initials: KA
authors.lastname: Rogers
authors.forename: M
authors.initials: M
authors.lastname: Sandig
authors.forename: N H
authors.initials: NH
authors.lastname: McKee
authors.forename: V I
authors.initials: VI
authors.lastname: Kalnins
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000293:injuries; D002404:Catheterization; D002465:Cell Movement / Q000502:physiology; D003599:Cytoskeleton / Q000648:ultrastructure*; D004730:Endothelium, Vascular / Q000293:injuries / Q000648:ultrastructure*; D005260:Female; D005455:Fluorescent Antibody Technique; D011817:Rabbits; D014945:Wound Healing / Q000502:physiology*