title: Assembly of 2-D membrane protein crystals: dynamics, crystal order, and fidelity of structure analysis by electron microscopy.
abstract: Membrane protein reconstitution into two-dimensional (2-D) ordered arrays is described. The assembly of 2-D crystals may be modeled as a two-step process: the membrane protein is first integrated in the lipid bilayer and then crystallized by removal of excess detergent or lipid and/or by precipitating agents. Lipid-detergent, protein-detergent, and lipid-protein interactions are critical during the first step, while lipid-protein and protein-protein interactions dominate events in the second step. The evidence supporting this model results from quasielastic light scattering analyses and electron microscopy of different lipid-detergent systems and reconstitution experiments with Escherichia coli porin OmpF, Phormidium laminosum photosystem I reaction centers, and integral membrane proteins of mammalian lens fiber cells.
authors.affiliation: M.E. Müller-Institute for High-Resolution Electron Microscopy at the Biocenter, Univeristy of Basel, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Engel
authors.forename: A
authors.initials: A
authors.lastname: Hoenger
authors.forename: A
authors.initials: A
authors.lastname: Hefti
authors.forename: C
authors.initials: C
authors.lastname: Henn
authors.forename: R C
authors.initials: RC
authors.lastname: Ford
authors.forename: J
authors.initials: J
authors.lastname: Kistler
authors.forename: M
authors.initials: M
authors.lastname: Zulauf
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000648:ultrastructure; D003459:Crystallins / Q000737:chemistry / Q000648:ultrastructure; D003460:Crystallization; D003902:Detergents; D008055:Lipids; D008565:Membrane Proteins / Q000737:chemistry / Q000648:ultrastructure*; D008823:Micelles; D008854:Microscopy, Electron; D008958:Models, Molecular; D015394:Molecular Structure; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000648:ultrastructure; D045331:Photosystem I Protein Complex; D018272:Porins
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003459:Crystallins; D003902:Detergents; D008055:Lipids; D008565:Membrane Proteins; D008823:Micelles; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D018272:Porins