Mutations Pro----Ala-35 and Tyr----Phe-75 of Rhodobacter capsulatus ferrocytochrome c2 affect protein backbone dynamics: measurements of individual amide proton exchange rate constants by 1H-15N HMQC spectroscopy.
Comparisons of hydrogen-deuterium solvent exchange rate constants for the NH protons of wild-type Pro----Ala-35 (P35A) and Tyr----Phe-75 (Y75F) Rhodobacter capsulatus ferrocytochromes c2 were made by 1H-15N heteronuclear multiple-quantum correlation spectroscopy. Exchange rate constants increased for the NH protons of residues 45-46, 54, 57-58, 60-61, 82-87, 98, and 100 with Y75F and 16-18, 20, 34, 37, 43, 45-46, and 58 with P35A. The increases in exchange rate constants are consistent with changes in unfolding equilibria and protein dynamics. In Y75F the exchange rate constants of the observable NH protons of the helix spanning Pro-79-Asp-89, namely Phe-82-Leu-87, increase to a similar degree, suggesting that this helix is a single cooperative unfolding unit compatible with the local unfolding model. As the oxidation-reduction potential of Y75F is 59 mV lower than wild-type cytochrome c2 (367 mV), the dynamic changes in this mutant, compared to wild-type, are proposed to be important determinants of the oxidation-reduction potential. Several differences between wild-type and Y75F are in common with P35A, a mutation which does not affect the oxidation-reduction potential, implying that not all observed dynamic changes are functionally important.
Gooley
P R
PR
Department of Pharmaceutical Sciences, College of Pharmacy, University of Arizona, Tucson 85721.
Caffrey
M S
MS
Cusanovich
M A
MA
MacKenzie
N E
NE
D000577:Amides / Q000737:chemistry*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000235:genetics*; D003574:Cytochrome c Group / Q000737:chemistry / Q000235:genetics*; D045362:Cytochromes c2; D003903:Deuterium; D006859:Hydrogen; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009154:Mutation; D009587:Nitrogen Isotopes; D010084:Oxidation-Reduction; D011487:Protein Conformation; D016354:Rhodobacter capsulatus / Q000201:enzymology / Q000235:genetics*; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D000577:Amides; D000596:Amino Acids; D003574:Cytochrome c Group; D009587:Nitrogen Isotopes; D006859:Hydrogen; D045362:Cytochromes c2; D003903:Deuterium