title: [Cell-mediated immune reactivity to sperm in disorders of human reproduction].
abstract: Besides the pathological anti-sperm humoral immunity, pathological anti-sperm cell-mediated immunity is considered as a crucial facet of the disturbances of human reproduction (male and female infertility, recurrent abortions, endometriosis, late EPH gestosis, fetal hypotrophy). A precise and objective method is designed, based on a one-step agarose Leukocyte Migration Inhibition Factor assay. The migration areas are evaluated by a computer-assisted image analysis system. Optimal concentrations of leukocytes and sperm, as well as technical conditions are described. The Radius Migration Indexes and Area Migration Indexes are computed and expressed as a Migration Index percentage for each patient or control. Preliminary clinical results indicate a highly significant association between leukocyte migration inhibition and cases of "immunopathological" infertility and repeated fetal loss.
authors.affiliation: Ustav pro péci o matku a dítĕ, Praha.
authors.forename: D G
authors.initials: DG
authors.lastname: Dimitrov
authors.forename: R
authors.initials: R
authors.lastname: Sedlák
authors.forename: K
authors.initials: K
authors.lastname: Nouza
authors.forename: R
authors.initials: R
authors.lastname: Kinský
mesh_terms: D002464:Cell Migration Inhibition; D005260:Female; D006801:Humans; D007246:Infertility / Q000276:immunology*; D008297:Male; D013094:Spermatozoa / Q000276:immunology*
vernacular_title: Imunitní reaktivita proti spermiím zprostredkovaná bunkami (BI) v poruchách lidské reprodukce.