[Enhancement of the quality of hepatic graft by restoration of hepatic glycogen reserves in the donor].
It has been suggested that depletion of donor hepatic glycogen reserves deleteriously affects the resistance of the hepatic graft to ischemic episodes. In this study, performed in the pig model, we showed that it is possible to enhance the quality of the graft at the time of reperfusion by using a method which rapidly restores the donor hepatic glycogen reserves. With the aid of an isolated liver perfusion model, we compared grafts (n = 24) harvested from pigs fed (group N), fasted for 24h (group J), or fasted with a restoration of glycogen reserves (group P). After the grafts were subjected to 8 hours of cold ischemia, the release of alanine aminotransferase, aspartate aminotransferase and lactic dehydrogenase in the perfusate increased in group J (P < 0.05 vs group N); the increase was corrected in group P (P < 0.05 vs group J). When the grafts were subjected to 15 minutes warm ischemia prior to the liver harvest, the production of bile was reduced in group J (P < 0.05 vs group N); bile production was reestablished in group P (P < 0.05 vs group J). The clinical application of such a method of donor nutritional conditioning, in the hours which precede organ harvesting, may enhance the quality of the hepatic graft at the time of transplantation.
Pattou
F
F
Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg.
Boudjema
K
K
Kerr-Conte
J
J
Barguil
Y
Y
Friese
N
N
Wolf
P
P
Jaeck
D
D
Cinqualbre
J
J
D000818:Animals; D005632:Fructose / Q000008:administration & dosage / Q000627:therapeutic use; D005947:Glucose / Q000008:administration & dosage / Q000627:therapeutic use; D005973:Glutamine / Q000008:administration & dosage / Q000627:therapeutic use; D008112:Liver Glycogen / Q000378:metabolism*; D016031:Liver Transplantation / Q000379:methods*; D009926:Organ Preservation / Q000379:methods*; D013552:Swine; D014019:Tissue Donors
D008112:Liver Glycogen; D005973:Glutamine; D005632:Fructose; D005947:Glucose
Amélioration de la qualité du greffon hépatique par la restauration des réserves hépatiques en glycogène chez le donneur.