title: Lack of specific binding of anticardiolipin antibodies to intact platelets.
abstract: The aims of this study were to investigate whether anticardiolipin antibodies (aCL) bind to intact (resting or activated) platelets in vitro. Suspensions of resting, activated (with a mixture of thrombin and collagen) and freeze-thawed platelets from healthy subjects were incubated with either affinity-purified aCL or pooled normal human immunoglobulin G (IgG). Platelet-bound IgG was measured by flow cytometric analysis of platelets incubated with a fluorescein-conjugated polyclonal goat anti-human IgG. There was no significant binding of IgG aCL to intact resting or activated platelets, while significant specific binding to freeze-thawed platelets was demonstrated. These results question the theory that aCL bind/activate intact platelets in vivo.
authors.affiliation: Lupus Arthritis Research Unit, Rayne Institute, St Thomas' Hospital, London, UK.
authors.forename: H J
authors.initials: HJ
authors.lastname: Haga
authors.forename: C
authors.initials: C
authors.lastname: Christopoulos
authors.forename: S J
authors.initials: SJ
authors.lastname: Machin
authors.forename: M
authors.initials: M
authors.lastname: Khamashta
authors.forename: G R
authors.initials: GR
authors.lastname: Hughes
mesh_terms: D000328:Adult; D017153:Antibodies, Anticardiolipin / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008297:Male; D015539:Platelet Activation; D010974:Platelet Aggregation
chemical_list: D017153:Antibodies, Anticardiolipin; D007074:Immunoglobulin G