Detection of antibodies to HCV: comparison of a c100-3 EIA and the 2nd generation EIA.
The reactivity of 558 serum specimens in Abbott 1st and 2nd generation EIAs was compared. A significant increase in HCV seropositivity was especially found in high risk groups such as hemophiliacs (57.7 vs. 73.1%), IVDA (54.5 vs. 61.8%), HIV positives (21.8 vs. 25.5%) or patients on dialysis (15.7 vs. 16.9%). Reactivity of all sera which only reacted in the 2nd generation EIA was 'confirmed' by using supplemental research assays. In contrast, sera which were highly reactive in the c100-3 assay but could not be confirmed by Neutralization Assay were weakly positive or negative in the 2nd generation test.
Polywka
S
S
Institute of Medical Microbiology and Immunology, University Hospital Eppendorf, Hamburg, FRG.
Kaars-Wiele
P
P
Schroeter
E
E
Mandler
J
J
Laufs
R
R
D006467:Hemophilia A / Q000276:immunology; D006508:Hepatitis Antibodies / Q000032:analysis*; D006526:Hepatitis C / Q000175:diagnosis / Q000276:immunology / Q000517:prevention & control*; D018937:Hepatitis C Antibodies; D006801:Humans; D007124:Immunoenzyme Techniques; D011237:Predictive Value of Tests; D006435:Renal Dialysis; D012307:Risk Factors
D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies