title: [Absorption of human serum antibodies against Toxoplasma gondii using Tachyzoites from the RH strain].
abstract: Absorption experiments to determine the number of T. gondii tachyzoites to extract the anti-Toxoplasma antibodies present in the human serum were carried out. The antibodies titer from each serum (n = 4) was determined by means of the Sabin-Feldman test (dye test). Ten millions of tachyzoites were sufficient to absorb the antibodies from sera presenting titer 1:16, 1:64 and 1:256. On the other side, the absorption of a 1:512 positive serum with 10 x 10(6) T. gondii decreased the titer to 1:64 and the dye test became negative when using 50 x 10(6) tachyzoites.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Chile, Santiago.
authors.forename: R
authors.initials: R
authors.lastname: Mercado
authors.forename: M
authors.initials: M
authors.lastname: Castro
authors.forename: J
authors.initials: J
authors.lastname: Sanhueza
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000302:isolation & purification*; D003429:Cross Reactions / Q000276:immunology; D051381:Rats; D014122:Toxoplasma / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan
vernacular_title: Absorción de anticuerpos séricos humanos anti-Toxoplasma gondii usando taquizoitos de la cepa RH.