Interrelations between steroid hormone secretion and morphological changes of porcine corpora lutea at various periods of luteal phase.
Four types of porcine corpora lutea (CL) were excised at 4 various stages of the luteal phase. One part of corpora lutea was homogenized and the concentration of progesterone (P4), estradiol (E2) and androgens (A) was assayed in adequate extracts. Second part of luteal cells obtained from each of four types of CL was cultured as separate monolayers and steroid secretion was measured after 24, 48 and 96 h of culture. The concentration of P4 in homogenates of CL type 2 and 3 was twice as high as that in type 1, while a decrease to minimum level in type 4 was observed. In cultures, maximum increase in P4 production was observed after 24 h, being the highest in type 1 (2800% of initial P4 concentration) and type 2 (320%) while in types 3 and 4 no increase was observed. The contents of androgens (A) in CL homogenates increased 3 times in type 2 and 3 and finally decreased in type 4 as compared with type 1. In cultures, maximum increase in A production was observed after 24 h, being the highest in type 1 (640% of A concentration prior to culture), while in types 2, 3 and 4 it was about 200%. The content of E2 in homogenates of CL types 1 and 2 was very low, but increased to about 200% in type 3 and to about 2500% in type 4.(ABSTRACT TRUNCATED AT 250 WORDS)
Gregoraszczuk
E L
EL
Department of Animal Physiology, Jagiellonian University, Krakow, Poland.
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008183:Luteal Phase / Q000502:physiology*; D011374:Progesterone / Q000378:metabolism*; D013552:Swine
D000728:Androgens; D011374:Progesterone; D004958:Estradiol