[DNA probe labeling with digoxigenin-dUTP and its application in gene diagnosis].
In this paper, DNA probe labeling by the randomly primed incorporation of digoxigenin-dUTP is reported. The sensitivity of color reaction and hybridization were 32 fg and 200 fg, respectively, and both were specific for the target. Single-copy and multi-copy gene fragments among 2 micrograms human genomic DNA were detected by beta IVS II, Fr 3-42 and 3'HVR labeled with digoxigenin-dUTP. The results were consistent with a radioactive control assay. This method has been successfully used in the gene diagnosis of adult polycystic kidney disease.
Liu
G
G
Institute of Basic Medical Sciences, Beijing.
D015342:DNA Probes; D003856:Deoxyuracil Nucleotides; D004076:Digoxigenin; D015894:Genome, Human; D006801:Humans; D009693:Nucleic Acid Hybridization; D007690:Polycystic Kidney Diseases / Q000175:diagnosis* / Q000235:genetics
D015342:DNA Probes; D003856:Deoxyuracil Nucleotides; D004076:Digoxigenin