Insulin-like growth factor I and II binding in human myometrium and leiomyomas.
OBJECTIVE
The purpose of this study is to determine if the human myometrium has receptors for insulin-like growth factors I and II and whether the concentration of these receptors is increased in leiomyomas.


METHODS
Specific binding of iodine 125-labeled insulin-like growth factor I and II was examined in the membrane preparations of myometrium and leiomyomas obtained from 10 women with uterine leiomyomas.


RESULTS
Binding studies indicate presence of specific binding sites for both insulin-like growth factors I and II in the myometrium and leiomyoma. The concentration of binding sites for insulin-like growth factor I, but not for insulin-like growth factor II, was significantly (p less than 0.01) higher in leiomyomas than in the myometrium. The dissociation constants for insulin-like growth factors I and II receptors in both myometrium and leiomyoma were similar.


CONCLUSIONS
insulin-like growth factor I, but not insulin-like growth factor II, receptors are increased in leiomyomas compared with those in myometrium, indicating that insulin-like growth factor I may play a role in the generation and/or growth of this tumor.
Chandrasekhar
Y
Y
Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston 77550.
Heiner
J
J
Osuamkpe
C
C
Nagamani
M
M
D000328:Adult; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D007889:Leiomyoma / Q000378:metabolism*; D008875:Middle Aged; D009215:Myometrium / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017451:Receptors, Somatomedin; D014594:Uterine Neoplasms / Q000378:metabolism*
D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II