title: Endothelin stimulates both cAMP formation and phosphatidylinositol hydrolysis in cultured embryonic bovine tracheal cells.
abstract: Embryonic bovine tracheal (EBTr) cells were found to possess receptors for endothelin (ET) of ET-1-selective (ETA) subtype with a Kd for ET-1 of 114 pM and a Bmax of 12.9 fmol/10(5) cells. Stimulation of EBTr cells with 100 pM to 100 nM ET-1 increased the contents of both inositol phosphates and cAMP in a concentration-dependent manner, indicating that the receptors are coupled to both phosphatidylinositol hydrolysis and cAMP formation in EBTr cells.
authors.affiliation: International Research Laboratories, Ciba-Geigy Ltd., Takarazuka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Oda
authors.forename: Y
authors.initials: Y
authors.lastname: Fujitani
authors.forename: T
authors.initials: T
authors.lastname: Watakabe
authors.forename: T
authors.initials: T
authors.lastname: Inui
authors.forename: T
authors.initials: T
authors.lastname: Okada
authors.forename: Y
authors.initials: Y
authors.lastname: Urade
authors.forename: E
authors.initials: E
authors.lastname: Okuda-Ashitaka
authors.forename: S
authors.initials: S
authors.lastname: Ito
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis*; D016232:Endothelins / Q000494:pharmacology*; D006868:Hydrolysis; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D011956:Receptors, Cell Surface / Q000378:metabolism; D017466:Receptors, Endothelin; D014132:Trachea / Q000166:cytology / Q000378:metabolism*
chemical_list: D016232:Endothelins; D010716:Phosphatidylinositols; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D000242:Cyclic AMP