Effect of sialoadenectomy on stomach lesions induced by indomethacin and ethanol in relation to gastric vascular permeability, the gastrin level and HCl secretion in rats.
Stomach lesions induced by indomethacin (20 mg.kg-1 i.p.) and ethanol (1 ml 95% intragastrically) were studied after a 24 hour fast in rats which had undergone sialoadenectomy. The size of the lesions was correlated with gastric HCl secretion, with gastric vascular permeability (determined from the Evans blue concentration in the stomach tissue after its i.v. administration) and with the serum gastrin level. These parameters were also studied in sialoadenectomized rats and in animals given epidermal growth factor (EGF) (50 lg.kg-1). It was found that sialoadenectomy significantly (p < 0.01) raised the incidence of stomach lesions after the administration of indomethacin and also after ethanol (p < 0.05). A significant increase in both basal and stimulated HCl secretion was found after sialoadenectomy. Both indomethacin and ethanol also increased gastric vascular permeability in rats not subjected to sialoadenectomy, but sialoadenectomy raised it significantly compared with the non-sialoadenectomized group. The serum gastrin levels fell after sialoadenectomy and the decrease was significant after the subsequent administration of indomethacin or ethanol. The administration of EGF to sialoadenectomized rats lowered the incidence of stomach lesions, inhibited HCl secretion and reduced vascular permeability. The lowered susceptibility of the gastric mucosa to the formation of lesions in sialoadenectomized rats given indomethacin or ethanol can be regarded as the outcome of the uptake of EGF.
Kohút
A
A
Department of Pharmacology, Faculty of Medicine, Safárik University, Kosice.
Maheĺová
O
O
Mojzís
J
J
Mirossay
L
L
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D000431:Ethanol / Q000009:adverse effects*; D005755:Gastrins / Q000097:blood*; D006851:Hydrochloric Acid / Q000378:metabolism*; D007213:Indomethacin / Q000009:adverse effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012469:Salivary Glands / Q000502:physiology*; D013270:Stomach / Q000098:blood supply; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism*
D005755:Gastrins; D000431:Ethanol; D004815:Epidermal Growth Factor; D006851:Hydrochloric Acid; D007213:Indomethacin