Deregulated c-fos augments the expression of IL-2 gene in T cells stimulated with anti-CD3 antibody.
The expression of IL-2 gene in T cells stimulated with immobilized anti-CD3 antibody is controlled by several transcription factors like as AP-1, IL-2 kappa B and NFAT. We studied an effect of deregulated c-fos, a component of AP-1, in the expression of IL-2 gene in T cells by using splenic T cells from transgenic mice carrying the exogenous c-fos gene under the control of the H2-Kb promoter (H2-c-fos). The IL-2 production of H2-c-fos T cells stimulated with anti-CD3 antibody was enhanced and prolonged. Levels of AP-1 and IL-2 kappa B transcription factors in the nuclear extract from the H2-c-fos T cells were also augmented. These results suggest that c-Fos is one of the major regulatory factors for the expression of IL-2 gene in T cells activated with anti-CD3 antibody.
Ochi
Y
Y
Department of Immunology, ICMR, Kobe University School of Medicine.
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001483:Base Sequence; D017252:CD3 Complex; D016762:Genes, fos / Q000502:physiology*; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D014157:Transcription Factors / Q000032:analysis
D000906:Antibodies; D017252:CD3 Complex; D007376:Interleukin-2; D014157:Transcription Factors