Growth factors promote inositol uptake in BC3H1 cells.
BC3H1 cells induced to differentiate by serum withdrawal were found to incorporate substantially less [3H]inositol into their phosphoinositides than cells induced to differentiate by growth in the presence of high serum. This decrease was found to be due to a decline in the rate of [3H]inositol uptake by the serum-starved cells. Addition of purified growth factors such as TGF-beta, EGF and FGF to these cells promoted inositol uptake and lead to an increase in the incorporation of [3H]inositol into phosphoinositides. Stimulation of inositol uptake by TGF-beta required at least a 24 hr exposure to the growth factor. These data indicate that growth factors regulate phosphoinositide metabolism at many different levels including at the level of inositol uptake.
Spizz
G
G
Dept. of Biochemistry and Molecular Biophysics, Howard Hughes Medical Inst., Washington University School of Medicine, St. Louis, MO 63110.
Pike
L J
LJ
D000818:Animals; D001692:Biological Transport; D002038:Bungarotoxins / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D007294:Inositol / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D010716:Phosphatidylinositols / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014316:Tritium
D002038:Bungarotoxins; D006133:Growth Substances; D007328:Insulin; D010716:Phosphatidylinositols; D011950:Receptors, Cholinergic; D016212:Transforming Growth Factor beta; D014316:Tritium; D007294:Inositol; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor