[The isolation of highly purified bacillary ribonucleases from recombinant strains of Escherichia coli].
Active and inactive highly purified extracellular alkaline ribonucleases (barnase) from recombinant Escherichia coli have been prepared according to the industrial technology of Bacillus intermedius ribonuclease purification. Electrophoretic parameters and ultraviolet spectra characterize these preparations as homogeneous proteins. Immunochemical test system of identification of inactive RNAse for its purification has been worked out.
Balaban
N P
NP
Sharipova
M R
MR
Vershinina
V I
VI
Znamenskaia
L V
LV
Chernokal'skaia
E B
EB
Romakhina
E R
ER
Volkova
T I
TI
Lopatnev
S V
SV
D000818:Animals; D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D007114:Immunization; D005779:Immunodiffusion; D010957:Plasmids; D011817:Rabbits; D011995:Recombination, Genetic; D012260:Ribonucleases / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D001426:Bacterial Proteins; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease
Poluchenie vysokoochishchennykh batsilliarnykh ribonukleaz iz rekombinantnykh shtammov Escherichia coli.