4-Aminopyridine and tetraethylammonium-induced changes in action potentials of unmyelinated axons.
The effects of different concentrations of 4-aminopyridine (4-AP) and tetraethylammonium (TEA) on the intra- and extracellular action potentials (ICAPs, ECAPs) of unmyelinated axons of Lumbricus terrestris were studied. The results showed different sensitivity of the axons to both potassium current blockers (4-AP and TEA), added to the medium. 4-AP led to spontaneous and single stimulus- evoked repetitive activity, manifested as a slow "burst" action potential propagation like "plateau" ICAP with oscillations. The ICAP duration of the TEA-treated axons increased mainly at the expense of the repolarization phase which reflected the increased duration of the ECAP recorded at long radial distances. The amplitude of the ICAPs after treatment with both blockers was decreased to 20% as compared to the controls (untreated axons) and ECAPs decreased to 40% in the TEA-treated axons. The conduction velocity (CV) of the action potentials was not significantly changed. The calculated total ionic current during the action potential upon TEA treatment was decreased and the duration of the outward phase was prolonged.
Radicheva
N I
NI
Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
Kolev
V B
VB
D015761:4-Aminopyridine / Q000494:pharmacology*; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D004558:Electric Stimulation; D005110:Extracellular Space / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D008839:Microelectrodes; D009186:Myelin Sheath / Q000502:physiology; D009431:Neural Conduction / Q000187:drug effects; D009835:Oligochaeta / Q000502:physiology*; D015221:Potassium Channels / Q000187:drug effects; D013757:Tetraethylammonium Compounds / Q000494:pharmacology*
D015221:Potassium Channels; D013757:Tetraethylammonium Compounds; D015761:4-Aminopyridine