Establishment and characterization of a new human extrahepatic bile duct carcinoma cell line (KMBC).
A new human extrahepatic bile duct carcinoma cell line (KMBC) was established from a serially transplanted tumor in nude mice that originated from a surgically resected tumor from a 73-year-old Japanese man; the cell line has been maintained for 5 five years. KMBC cells proliferate in a monolayered sheet with a population doubling time of 30 hours. Chromosome number was distributed in a range from 37 to 44, with modal numbers of 40 and 41. KMBC cells and the reconstituted tumor in a nude mouse showed moderately to poorly differentiated adenocarcinoma and possessed various functional characteristics of extrahepatic bile duct carcinoma. KMBC cells secreted carbohydrate antigen 19-9, tissue polypeptide antigen, carcinoembryonic antigen, ferritin, beta 2-microglobulin, fibronectin, and alpha 2-macroglobulin and produced glutamic oxaloacetic transaminase and alkaline phosphatase. KMBC is the second established cell line that originated from a human extrahepatic bile duct carcinoma in the world literature, and it will be applicable to various experiments.
Yano
H
H
First Department of Pathology, Kurume University School of Medicine, Japan.
Maruiwa
M
M
Iemura
A
A
Mizoguchi
A
A
Kojiro
M
M
D002759:Adenoma, Bile Duct / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D000818:Animals; D001650:Bile Duct Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D002453:Cell Cycle; D003470:Culture Media; D016895:Culture Media, Serum-Free; D006500:Hepatic Duct, Common; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009174:Mycoplasma / Q000302:isolation & purification; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D001798:Blood Proteins; D003470:Culture Media; D016895:Culture Media, Serum-Free; D009363:Neoplasm Proteins