title: Diagnosis of human parvovirus B19 infections by polymerase chain reaction.
abstract: The polymerase chain reaction (PCR) was used for detecting parvovirus B19 DNA in clinical specimens. A pair of oligonucleotide primers spanning the PstI-fragment of the B19 virus genome was used for PCR, and a PCR product of 727 bp was amplified. B19 virus DNA was detected in all sera (n = 26) of individuals in the incubation period and acute phase of infection. PCR was useful for detecting viral B19 DNA in amniotic fluid and fetal blood of hydropic fetuses, confirming fetal B19 virus infection.
authors.affiliation: Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Munich, Germany.
authors.forename: T F
authors.initials: TF
authors.lastname: Schwarz
authors.forename: G
authors.initials: G
authors.lastname: Jäger
authors.forename: W
authors.initials: W
authors.lastname: Holzgreve
authors.forename: M
authors.initials: M
authors.lastname: Roggendorf
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D016731:Erythema Infectiosum / Q000175:diagnosis*; D005260:Female; D006801:Humans; D015160:Hydrops Fetalis / Q000382:microbiology; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis
chemical_list: D004279:DNA, Viral