Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3.
A total of 116,118 basepairs (bp) derived from three cosmids spanning the ERCC1 locus of human chromosome 19q13.3 have been sequenced with automated fluorescence-based sequencers and analysed by polymerase chain reaction amplification and computer methods. The assembled sequence forms two contigs totalling 105,831 bp, which contain a human fosB proto-oncogene, a gene encoding a protein phosphatase, two genes of unknown function and the previously-characterized ERCC1 DNA repair gene. This light band region has a high average density of 1.4 Alu repeats per kilobase. Human chromosome light bands could therefore contain up to 75,000 genes and 1.5 million Alu repeats.
Martin-Gallardo
A
A
Receptor Biochemistry and Molecular Biology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
McCombie
W R
WR
Gocayne
J D
JD
FitzGerald
M G
MG
Wallace
S
S
Lee
B M
BM
Lamerdin
J
J
Trapp
S
S
Kelley
J M
JM
Liu
L I
LI
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19; D003360:Cosmids; D004247:DNA / Q000032:analysis / Q000235:genetics*; D015870:Gene Expression; D016762:Genes, fos; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D000090063:Proto-Oncogene Mas; D016760:Proto-Oncogene Proteins c-fos; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D001426:Bacterial Proteins; C064269:FOSB protein, human; C483819:Fosb protein, mouse; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016760:Proto-Oncogene Proteins c-fos; D004247:DNA