Quantitative preservation of viability of Aspergillus fumigatus.
Quantitative preservation of the viability of two isolates of Aspergillus fumigatus was studied over a 15-month period of storage. Significant loss of viability occurred in isolates preserved at -70 degrees C in both phosphate-buffered saline with Tween (PBST) and 0.1% gelatin, immediately, and between 6 and 15 months. Storage in 10% and 25% dimethyl sulphoxide at -70 degrees C was successful up to 8 weeks with gradual loss of viability later. PBST at 4 degrees C or room temperature, or 10% glycerol at -70 degrees C maintained 100% viability of the cultures up to 6 months and 15 months, respectively. To preserve 100% viability, long-term storage of A. fumigatus is best done in 10% glycerol in PBST at -70 degrees C compared with the other methods tested.
Denning
D W
DW
Department of Medicine, Santa Clara Valley Medical Center, CA.
Clemons
K V
KV
Stevens
D A
DA
D001232:Aspergillus fumigatus / Q000254:growth & development*; D003470:Culture Media; D011446:Prospective Studies; D013696:Temperature
D003470:Culture Media