Studies with biotinylated RBC: (1) use of flow cytometry to determine posttransfusion survival and (2) isolation using streptavidin conjugated magnetic beads.
Methods are reported for the quantitation and isolation of biotinylated red blood cells (B- RBC). The first method is for determination of posttransfusional survival of rabbit RBC by flow cytometry. The survival of B-RBC was measured using both fresh and paraformaldehyde-fixed cells with similar results. The posttransfusion survival of rabbit RBC measured in this way was normal. There was no indication of increased cell destruction due to antibodies directed against B-RBC and no evidence for loss of biotin from circulating cells. The second methodology is for the isolation of B-RBC from blood with streptavidin-coated magnetic beads. At least eighty percent of positive cells were recovered with very few false positives. Both methods may be helpful in the study of resealed erythrocytes.
Russo
V
V
Istituto di Fisiologia Veterinaria e Biochimica, Milan, Italy.
Barker-Gear
R
R
Gates
R
R
Franco
R
R
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D001710:Biotin; D001803:Blood Transfusion; D002469:Cell Separation / Q000379:methods*; D004905:Erythrocyte Aging / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D008280:Magnetics; D008863:Microspheres; D011817:Rabbits; D019809:Streptavidin; D013997:Time Factors
D001426:Bacterial Proteins; D001710:Biotin; D019809:Streptavidin