title: Coordinate pretranslational control of cAMP-dependent protein kinase subunit expression during development in the water mold Blastocladiella emersonii.
abstract: The aquatic fungus Blastocladiella emersonii provides a system for studying the regulation of expression of regulatory (R) and catalytic (C) subunits of cAMP-dependent protein kinase (PKA). Blastocladiella cells contain a single PKA with properties very similar to type II kinases of mammalian tissues. During development cAMP-dependent protein kinase activity and its associated cAMP-binding activity change drastically. We have previously shown that the increase in cAMP-binding activity during sporulation is due to de novo synthesis of R subunit and to an increase in the translatable mRNA coding for R (Marques et al., Eur. J. Biochem. 178, 803, 1989). In the present work we have continued these studies to investigate the mechanism by which the changes in the level of kinase activity take place. The C subunit of Blastocladiella has been purified; antiserum has been raised against it and used to determine amounts of C subunit throughout the fungus' life cycle. A sharp increase in C subunit content occurs during sporulation and peaks at the zoospore stage. Northern blot analyses, using Blastocladiella C and R cDNA probes, have shown that the levels of C and R mRNAs parallel their intracellular protein concentrations. These results indicate a coordinate pretranslational control for C and R subunit expression during differentiation in Blastocladiella.
authors.affiliation: Departamento de Bioquímica, Universidade de São Paulo, Brasil.
authors.forename: M do V
authors.initials: Mdo V
authors.lastname: Marques
authors.forename: A C
authors.initials: AC
authors.lastname: Borges
authors.forename: J C
authors.initials: JC
authors.lastname: de Oliveira
authors.forename: S L
authors.initials: SL
authors.lastname: Gomes
mesh_terms: D001754:Blastocladiella / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D015152:Blotting, Northern; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics; D000242:Cyclic AMP / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D046911:Macromolecular Substances; D000242:Cyclic AMP; D011494:Protein Kinases