title: Induction of neointima formation by platelet derived angiogenesis fraction in a small diameter, wide pore, PTFE graft.
abstract: Enhancement of endothelialization and patency of a small diameter (2 mm), wide pore, PTFE graft was attempted by coating the luminal surface with a platelet derived angiogenesis fraction (PDAF) and implanting it in a rat model. PDAF was delivered to the grafts by combining it with a carrier polymer. PDAF-treated grafts were initially implanted in the retroperitoneum for 21 days followed by removal of one for histology and in situ end to side bypass to the infrarenal aorta for the other. Vascularized grafts were examined at 14 days for patency and 100 days for patency and histology. Significant differences were noted in transmural ingrowth of capillaries and tissue at 21 days post implantation in PDAF-treated versus untreated grafts. Similarly, near significance was noted in capillary ingrowth and significance was noted in tissue ingrowth at 100 days in PDAF-treated grafts. Despite favorable trends particularly early in the time course, no significant differences in graft patency, endothelialization, or hydroxyproline content was demonstrated between PDAF-treated and untreated grafts. Results of this preliminary study are encouraging for further study of PDAF-treated PTFE grafts and the potential that rapid vascularized neointima formation results improved in graft patency rates.
authors.affiliation: Department of Surgery, University of Minnesota, Minneapolis.
authors.forename: M D
authors.initials: MD
authors.lastname: Lado
authors.forename: D R
authors.initials: DR
authors.lastname: Knighton
authors.forename: M
authors.initials: M
authors.lastname: Cavallini
authors.forename: V D
authors.initials: VD
authors.lastname: Fiegel
authors.forename: C
authors.initials: C
authors.lastname: Murray
authors.forename: G D
authors.initials: GD
authors.lastname: Phillips
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D001807:Blood Vessel Prosthesis; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D006909:Hydroxyproline / Q000032:analysis; D008297:Male; D009389:Neovascularization, Pathologic; D011138:Polytetrafluoroethylene; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014654:Vascular Patency
chemical_list: D016212:Transforming Growth Factor beta; D011138:Polytetrafluoroethylene; D006909:Hydroxyproline