Assembly of a hybrid from the alpha subunit of Na+/K(+)-ATPase and the beta subunit of H+/K(+)-ATPase.
Messenger RNA for the alpha subunit of Torpedo californica Na+/K(+)-ATPase was injected into Xenopus oocytes together with that of the beta subunit of rabbit H+/K(+)-ATPase. The Na+/K(+)-ATPase alpha subunit was assembled in the microsomal membranes with the H+/K(+)-ATPase beta subunit, and became resistant to trypsin. These results suggest that the H+/K(+)-ATPase beta subunit facilitates the stable assembly of the Na+/K(+)-ATPase alpha subunit in microsomes.
Noguchi
S
S
Department of Biology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Maeda
M
M
Futai
M
M
Kawamura
M
M
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D005260:Female; D017506:H(+)-K(+)-Exchanging ATPase; D046911:Macromolecular Substances; D008970:Molecular Weight; D009865:Oocytes / Q000502:physiology; D010957:Plasmids; D055503:Protein Multimerization; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014101:Torpedo; D014357:Trypsin; D014982:Xenopus laevis
D046911:Macromolecular Substances; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014357:Trypsin; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase; D000254:Sodium-Potassium-Exchanging ATPase