Amyloid precursor protein mRNA encoding the Kunitz protease inhibitor domain is increased by kainic acid-induced seizures in rat hippocampus.
A 168-nucleotide exon, found in alternatively spliced amyloid precursor protein (APP) mRNAs, encodes a Kunitz protease inhibitor (KPI) domain. Kainic acid (ip) caused a selective increase of KPI mRNA in rat hippocampus. By in situ hybridization, KPI mRNA was induced in the neuronal layers of the hippocampus 11-12 h after the onset of kainate-induced seizures. The kainate-induced elevation of the KPI-containing APP-770 mRNA was blocked by pretreatment with the anticonvulsant pentobarbital. These data suggest that kainate-induced seizures cause alterations in APP RNA stability and/or processing in rat hippocampal neurons.
Willoughby
D A
DA
Ethel Percy Andrus Gerontology Center, University of Southern California, Los Angeles 90089-0191.
Johnson
S A
SA
Pasinetti
G M
GM
Tocco
G
G
Najm
I
I
Baudry
M
M
Finch
C E
CE
D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000378:metabolism*; D017403:In Situ Hybridization; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D010424:Pentobarbital / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000235:genetics*; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000235:genetics*
D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger; D014360:Trypsin Inhibitor, Kunitz Soybean; D010424:Pentobarbital; D007608:Kainic Acid