title: Alteration of pituitary-adrenal responses to adrenalectomy by the immunological targeting of CRF neurons.
abstract: We previously demonstrated that cellular toxins added to a cytotoxic IgG2a monoclonal antibody to corticotropin releasing factor (CRF-MAb) may specifically penetrate some hypothalamic CRF neurons, after central injection near the paraventricular nuclei. We attempt here to evaluate the consequential effects on the CRF neurons functioning. Such a toxic mix, 4 weeks after its central injection, caused a marked reduction (66%) of the chronic adrenocorticotropic hormone (ACTH) release in response to a bilateral adrenalectomy (7th day). This change was accompanied by a reduction in the CRF concentration (43%) measured in the median eminence. We concluded that specific internalization of toxins, by the way of CRF-MAb, leads to a long-term dysregulation of the CRF synthesis and/or neuronal transport.
authors.affiliation: Laboratory of Cellular Biology, INSERM U.308, Nancy, France.
authors.forename: F
authors.initials: F
authors.lastname: Menzaghi
authors.forename: A
authors.initials: A
authors.lastname: Burlet
authors.forename: M
authors.initials: M
authors.lastname: Chapleur
authors.forename: J P
authors.initials: JP
authors.lastname: Nicolas
authors.forename: C
authors.initials: C
authors.lastname: Burlet
mesh_terms: D000315:Adrenalectomy; D000324:Adrenocorticotropic Hormone / Q000097:blood / Q000378:metabolism*; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology*; D007031:Hypothalamus / Q000502:physiology*; D008297:Male; D009474:Neurons / Q000502:physiology*; D010913:Pituitary-Adrenal System / Q000502:physiology*; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012016:Reference Values
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; C041645:pro-corticotropin releasing hormone; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone