title: Standards and controls in DNA assays.
abstract: Automated methods are described that begin to standardize genetic analysis at the DNA level. These new methods complement existing automation such as DNA extraction, liquid-handling robotics, thermal cycling and computer information management systems. Using electrophoresis, several independent genetic loci can be analyzed simultaneously using multi-color fluorescent gel scanning detection where DNA segments labeled with up to four different fluorescent dyes are analyzed within a single electrophoresis lane. The amounts and molecular lengths of PCR products are determined with high accuracy and precision by co-electrophoresis with a uniquely colored internal standard. These new methods have the potential of minimizing variations in results among different laboratories.
authors.affiliation: Applied Biosystems Inc., Foster City, CA 94404.
authors.forename: P E
authors.initials: PE
authors.lastname: Mayrand
mesh_terms: D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005820:Genetic Testing / Q000379:methods*; D066298:In Vitro Techniques; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards
chemical_list: D004247:DNA