Altered expression of blood group A and H antigens on red cells from an acute leukemic patient.
Alternate expressions of the blood group A and H antigens on red cells are described in a patient with acute myelocytic leukemia. The patient's red cells showed mixed field agglutination with anti-A and anti-H sera and lectins, and no agglutination with anti-B serum. The agglutinability of the A red cells with Dolichos biflorus lectin was between A1 and A2 (A intermediate). Inagglutinable red cells were separated with anti-A agglutinin, and the proportion was about 80% of total cells. The agglutinating activity with Ulex europaeus anti-H of red cells, which were inagglutinable with anti-A, was 16 times weaker than that of group O cells. The weaker reaction with Ricinus communis lectin and the stronger reaction with Psathyrella velutina lectin on the inagglutinable cells with anti-A than those on the group O cells suggest that fucosyl alpha (1-2) and galactosyl beta (1-4) residues at the nonreducing end of carbohydrate chains of H antigens on the red cells were diminished, and N-acetylglucosaminyl beta (1-3) residues were sequentially exposed. His saliva contained A and H substances in normal amounts of a secretor. Serum alpha-N-acetylgalactosaminyltransferase activity which converts O red cells to A red cells was the same as those in sera from A1 individuals. These results suggest that the synthesis of H precursors is partially blocked in this patient's red cells.
Matsuki
T
T
Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Shimano
S
S
Furukawa
K
K
D000017:ABO Blood-Group System / Q000096:biosynthesis / Q000737:chemistry; D004912:Erythrocytes / Q000276:immunology; D005646:Fucosyl Galactose alpha-N-Acetylgalactosaminyltransferase / Q000097:blood; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins; D015470:Leukemia, Myeloid, Acute / Q000097:blood*; D008297:Male; D008875:Middle Aged
D000017:ABO Blood-Group System; D037102:Lectins; D005646:Fucosyl Galactose alpha-N-Acetylgalactosaminyltransferase