title: A biosensor for L-proline determination by use of immobilized microbial cells.
abstract: A biosensor to quantify L-proline within 10(-5)-10(-3) mole/L concentration is described. Immobilized Pseudomonas sp. cells grown in a medium containing L-proline as the only source of carbon and nitrogen were used to create the biosensor. The cells oxidized L-proline specifically consuming O2 and did not react with other amino acids and sugars. The change in oxygen concentration was detected with a Clark oxygen membrane electrode. The cells were immobilized by entrapment in polyvinyl alcohol (PVA) cryogel. The resultant biocatalyst had a high mechanical strength and retained its L-proline-oxidizing ability for at least two months.
authors.affiliation: Yerevan Physics Institute, Armenia.
authors.forename: A L
authors.initials: AL
authors.lastname: Simonian
authors.forename: E I
authors.initials: EI
authors.lastname: Rainina
authors.forename: V I
authors.initials: VI
authors.lastname: Lozinsky
authors.forename: I E
authors.initials: IE
authors.lastname: Badalian
authors.forename: G E
authors.initials: GE
authors.lastname: Khachatrian
authors.forename: S Sh
authors.initials: SSh
authors.lastname: Tatikian
authors.forename: T A
authors.initials: TA
authors.lastname: Makhlis
authors.forename: S D
authors.initials: SD
authors.lastname: Varfolomeyev
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D001431:Bacteriological Techniques / Q000295:instrumentation; D015374:Biosensing Techniques; D003470:Culture Media; D010101:Oxygen Consumption; D011392:Proline / Q000032:analysis*; D011549:Pseudomonas / Q000737:chemistry* / Q000254:growth & development
chemical_list: D003470:Culture Media; D000255:Adenosine Triphosphate; D011392:Proline