The biological interaction of cis- and trans-urocanic acid and DNA.
The potential for the cis and trans isomers of urocanic acid to produce DNA damage was measured by assays for DNA binding (32P-postlabeling assay), for induction of DNA repair (unscheduled DNA synthesis assay) and induction of mutations (Salmonella typhimurium and Escherichia coli plate-incorporation assays). These assays did not detect any evidence of a direct effect of either isomer of urocanic acid on DNA over a wide range of concentrations. These results suggest that neither isomer of urocanic acid alone, nor ultraviolet-irradiation of either cis or trans-urocanic acid produces significant DNA damage under conditions that permit cell survival.
Yarosh
D B
DB
Applied Genetics Inc., Freeport, New York 11520.
Gettings
S D
SD
Alas
L G
LG
Kibitel
J T
JT
San
R H
RH
Wagner
V O
VO
McEwen
G N
GN
D000818:Animals; D002417:Cattle; D002461:Cell Line, Transformed; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000187:drug effects; D004926:Escherichia coli; D005347:Fibroblasts; D006801:Humans; D008297:Male; D008862:Microsomes, Liver; D009154:Mutation / Q000187:drug effects; D010761:Phosphorus Radioisotopes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012486:Salmonella typhimurium; D013237:Stereoisomerism; D014466:Ultraviolet Rays; D014560:Urocanic Acid / Q000494:pharmacology*
D004269:DNA, Bacterial; D010761:Phosphorus Radioisotopes; D004247:DNA; D014560:Urocanic Acid