[Side chain cleavage of sterols by Mycobacterium sp. M12].
A mutant strain of Mycobacterium sp. M12 was obtained. It can convert cholesterol. 3 beta-acetyl-cholesterol, sitosterol and 3 beta-acetyl-sitosterol to 4-androstane-3,17-dione (4-AD) (main product, the yield is 69%, 70%, 26% and 22% respectively) and 1,4-androstadiene-3,17-dione (ADD) (little amount). Optimum conditions for the side chain cleavage of cholesterol and 3 beta-acetyl-cholesterol by Mycobacterium sp. M12 were studied.
Zhang
L Q
LQ
Shanghai Research Center of Biotechnology, Chinese Academy of Sciences.
Bian
E P
EP
Wang
Y
Y
D000730:Androstadienes / Q000378:metabolism; D000735:Androstenedione / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D005043:Etiocholanolone; D009161:Mycobacterium / Q000378:metabolism*; D012855:Sitosterols / Q000378:metabolism
D000730:Androstadienes; D012855:Sitosterols; C006573:1,4-androstadiene-3,17-dione; D000735:Androstenedione; D002784:Cholesterol; D005043:Etiocholanolone