title: Effect of estrogens on the adhesion of Trichomonas vaginalis to epithelial cells in vitro.
abstract: 1. To further characterize the basic aspects of parasite-cell interaction, untreated and hormone-treated Madin-Darby canine kidney (MDCK) cells were allowed to interact with T. vaginalis. 2. Among the six hormones assayed, alpha-estradiol caused MDCK cells to become highly adhesive to T. vaginalis. Increases in parasite adhesion of 83% and 68% were obtained by previous incubation of the epithelial monolayers for 18 h with 1 ng/ml alpha-estradiol and 10 ng/ml 17-beta-estradiol, respectively. 3. The estrogenic response of MDCK cells was inhibited by tamoxifen, cycloheximide, puromycin, or 2-deoxy-D-glucose. 4. We conclude that MDCK cells possess receptors for estrogens and that T. vaginalis cytoadherence can be influenced by the hormonal cycle of its host.
authors.affiliation: Departamento de Parasitologia e Biofísica Celular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
authors.forename: F C
authors.initials: FC
authors.lastname: Silva-Filho
authors.forename: V L
authors.initials: VL
authors.lastname: Bonilha
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D004285:Dogs; D004848:Epithelium / Q000187:drug effects / Q000469:parasitology; D004967:Estrogens / Q000494:pharmacology*; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology; D008854:Microscopy, Electron; D013997:Time Factors; D014246:Trichomonas vaginalis / Q000187:drug effects*
chemical_list: D004967:Estrogens