Expression of reporter genes introduced by microinjection and electroporation in fish embryos and fry.
The technique for foreign gene transfer in fish is becoming a novel method for genetic engineers to produce useful transgenic fish as well as for experimental purposes. Our studies of transgenic fish have been focused on methods of gene transfer and regulatory elements for transgene expression. I describe the characteristics of 3 gene transfer methods we have established (i.e., microinjection into fertilized eggs, microinjection into oocytes, and electroporation). Also described is a series of experiments to estimate activities of several promoters and enhancers in a fish cell line. Finally, experiments to examine activities of the elements shown to be active in the cell line in fish embryos and fry are described. SV2, miw, and metallothioneine promoters of trout and mouse were shown to be active in these experiments.
Inoue
K
K
Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Tokyo, Japan.
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D003584:Cytological Techniques; D004742:Enhancer Elements, Genetic / Q000502:physiology; D005399:Fishes / Q000235:genetics*; D015870:Gene Expression; D005819:Genetic Markers; D008845:Microinjections / Q000379:methods; D011401:Promoter Regions, Genetic / Q000502:physiology; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D005819:Genetic Markers; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase