title: Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase.
abstract: We have cloned and sequenced the Escherichia coli K-12 ppsA gene. The ppsA gene codes for PEP synthase, which converts pyruvate into phosphoenolpyruvate (PEP), an essential step in gluconeogenesis when pyruvate or lactate are used as a carbon source. The open reading frame consists of 792 amino acids and shows homology with other phosphohistidine-containing enzymes that catalyze the conversion between pyruvate and PEP. These enzymes include pyruvate, orthophosphate dikinases from plants and Bacteroides symbiosus and Enzyme I of the bacterial PEP:carbohydrate phosphotransferase system.
authors.affiliation: Institut für Biologie I, Rheinisch-Westfälische Technische Hochschule, Aachen, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Niersbach
authors.forename: F
authors.initials: F
authors.lastname: Kreuzaler
authors.forename: R H
authors.initials: RH
authors.lastname: Geerse
authors.forename: P W
authors.initials: PW
authors.lastname: Postma
authors.forename: H J
authors.initials: HJ
authors.lastname: Hirsch
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D006639:Histidine / Q000031:analogs & derivatives / Q000235:genetics; D008969:Molecular Sequence Data; D011772:Pyruvate, Orthophosphate Dikinase / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D006639:Histidine; D011772:Pyruvate, Orthophosphate Dikinase; C005436:phosphohistidine