A molecular deletion map of the Y chromosome long arm defining X and autosomal homologous regions and the localisation of the HYA locus to the proximal region of the Yq euchromatin.
41 Y-linked DNA probes that detect sequences on the Y chromosome long arm have been used to analyse genomic DNA from a series of 23 patients with deletions of Yq. Southern blot analysis has differentiated 15 distinct breakpoints, which divide Yq into 14 mapping intervals. From the pattern of DNA sequences present in each patient, it has been possible to produce a congruent deletion map, with the exception of two cases which are not compatible with the consensus order. These patients can be explained by the presence of inversion polymorphisms on Yq in the general population or by complex rearrangements induced during the formation of the deleted chromosomes. The distribution of sequences on the Y long arm has defined distinct regions of homology with autosomes, the Y short arm and the long and short arms of the X. A number of the patients have been typed for the presence or absence of H-Y antigen (as determined by the cytotoxic T-cell assay) and it has been possible, from analysis of informative cases, to assign the locus to the proximal region of the Yq euchromatin.
O'Reilly
A J
AJ
University of Cambridge, Department of Pathology, UK.
Affara
N A
NA
Simpson
E
E
Chandler
P
P
Goulmy
E
E
Ferguson-Smith
M A
MA
D002460:Cell Line; D002843:Chromatin; D002871:Chromosome Banding; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D017353:Gene Deletion; D015723:Gene Library; D015321:Gene Rearrangement; D006182:H-Y Antigen / Q000235:genetics*; D006801:Humans; D008297:Male; D017385:Sequence Homology; D012729:Sex Chromosome Aberrations / Q000235:genetics*; D014178:Translocation, Genetic; D014960:X Chromosome; D014998:Y Chromosome
D002843:Chromatin; D006182:H-Y Antigen; D004247:DNA