title: Comparative immunohistochemical identification and relative distribution of immunocompetent cells in sections of frozen or formalin-fixed tissue from human periapical inflammatory lesions.
abstract: The presence of immunocompetent cells (B, Th/i and Ts/c cells and macrophages) and the ratios of these cell populations in periapical lesions (radicular granulomas, radicular cysts and apical scars) were demonstrated immunohistochemically using paraffin and cryo-sections. Thirty-four human periapical lesions were examined for the presence of immunocompetent cells by monoclonal antibodies and the biotin-avidin-horseradish peroxidase method. The T/B cell ratio of radicular cysts was significantly higher than that of both radicular granulomas and apical scars, and the average number of Th/i cells was greater than that of Ts/c cells in the radicular granulomas. However, no significant difference was found between radicular cyst and apical scar. The number of macrophages in radicular granulomas was significantly higher than that of the other lesions. These findings suggested that periapical lesions develop as a result of both humoral and cell-mediated immunological response and indicated that the ratios of immunocompetent cells are different in the different types of periapical lesion.
authors.affiliation: Department of Endodontics, Kanagawa Dental College, Yokosuka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Tani
authors.forename: T
authors.initials: T
authors.lastname: Osada
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Umemoto
mesh_terms: D001402:B-Lymphocytes; D006801:Humans; D007124:Immunoenzyme Techniques; D007958:Leukocyte Count; D008264:Macrophages; D010485:Periapical Periodontitis / Q000276:immunology* / Q000473:pathology; D016176:T-Lymphocyte Subsets; D013601:T-Lymphocytes; D016707:Tissue Fixation