title: Insertion sequence IS1016 and absence of Haemophilus capsulation genes in the Brazilian purpuric fever clone of Haemophilus influenzae biogroup aegyptius.
abstract: Brazilian purpuric fever (BPF) strains of Haemophilus influenzae biogroup aegyptius form a clone of organisms distinct from more innocuous, conjunctivitis-associated isolates. There has been controversy over whether the virulence of BPF strains might derive from the presence of a polysaccharide capsule analogous to that found in conventional invasive H. influenzae, a controversy fuelled by the observation (G. M. Carlone, L. Gorelkin, L. L. Gheesling, A. L. Erwin, S. K. Hoiseth, M. H. O. Mulks, S. P. Connor, R. S. Weyant, J. Myrick, L. Rubin, R. S. Mumford III, E. H. White, R. J. Arko, B. Swaminathan, L. M. Graves, L. W. Mayer, M. K. Robinson, S. P. Caudill, and the Brazilian Purpuric Fever Study Group, J. Clin, Microbiol. 27:609-614, 1989) that a capsulation DNA probe from H. influenzae type b hybridized uniquely to BPF strains. In this work, the basis for this hybridization has been established as the possession by BPF strains, but not by non-BPF strains, of the Haemophilus insertion element IS1016. Although IS1016 is associated with the capsulation locus in some Haemophilus spp., a Southern hybridization study suggests that in BPF strains there are no capsulation genes.
authors.affiliation: Department of Paediatrics, University of Oxford, John Radcliffe Hospital, United Kingdom.
authors.forename: S R
authors.initials: SR
authors.lastname: Dobson
authors.forename: J S
authors.initials: JS
authors.lastname: Kroll
authors.forename: E R
authors.initials: ER
authors.lastname: Moxon
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D001938:Brazil; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000235:genetics*; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000235:genetics*; D018805:Sepsis / Q000209:etiology
chemical_list: D004251:DNA Transposable Elements; D011135:Polysaccharides, Bacterial