Permeability changes following ischemia-reperfusion injury in the rabbit hindlimb.
Changes in permeability following ischemia-reperfusion injury were assessed in the intact rabbit hindlimb by measuring the transvascular clearance of 125I-labeled rabbit serum albumin. Ischemia was induced for periods of 1 or 2 hours by use of a pneumatic tourniquet inflated to 300 mmHg. Following ischemia, the limb was reperfused for 1, 2, or 3 hours. The albumin clearance in the gastrocnemius muscle of control rabbits was 5.1 +/- 0.7 (mean +/- SEM) microliters/hr/g dry weight. Following 1 hour of ischemia and reperfusion, muscle albumin clearance rose to 71.4 +/- 26 microliters/hr/g dry weight which was not significantly different from those animals that underwent 2 hours of ischemia. Muscle albumin clearance continued to be elevated following 2 hours of reperfusion; however, it returned toward control levels after 3 hours of reperfusion. These data suggest there is a transient increase in albumin permeability following ischemia-reperfusion injury in skeletal muscle.
Kupinski
A M
AM
Department of Surgery, Albany Medical College, New York.
Shah
D M
DM
Bell
D R
DR
D000818:Animals; D002199:Capillary Permeability; D006614:Hindlimb; D007457:Iodine Radioisotopes; D009132:Muscles / Q000378:metabolism*; D011817:Rabbits; D015427:Reperfusion Injury / Q000378:metabolism*; D012709:Serum Albumin / Q000493:pharmacokinetics*
D007457:Iodine Radioisotopes; D012709:Serum Albumin