Simultaneous determination of coagulation and fibrinolysis parameters for diagnostics of disseminated intravascular blood coagulation (DIC).
A diagnostic method is described for determining the parameters of the human blood plasma coagulation and fibrinolysis by turbidimetry. Diluted plasma with thrombin and streptokinase is mixed to initiate clot formation and subsequent clot dissolution. The resultant profile of absorbance versus time is analysed to determine six parameters: plasma coagulation time, the rate of coagulation, fibrinogen concentration, the rate of fibrinolysis, fibrin clot half-lysis and lysis time. The assay is precise, sensitive and requires 0.1 ml plasma. The method has a good correlation with generally accepted haemostatic tests and allowed us to recognize the stage of DIC syndrome for less than 10 minutes. This new approach was successfully applied for studying the haemostasis in patients with acute intestinal infection.
Lezhen
T
T
Department of Enzyme Chemistry and Biochemistry, Institute of Biochemistry Acad Sci., Kiev, Ukraine.
Kramarev
S
S
Kolesnik
L
L
Kudinov
S
S
D001777:Blood Coagulation; D001780:Blood Coagulation Tests; D002675:Child, Preschool; D004211:Disseminated Intravascular Coagulation / Q000097:blood / Q000175:diagnosis*; D005342:Fibrinolysis; D006801:Humans; D007223:Infant; D013997:Time Factors