Gram scale purification and preparation of rabbit liver zinc metallothionein.
A simplified procedure for purifying gram quantities of rabbit liver metallothionein (MT) using gel filtration and anion exchange chromatography is presented. The MT purification made use of anion exchange batch elution chromatography which greatly shortened the procedure. Quantitation techniques for use with crude and purified MT are discussed. This paper also describes the preparation of large amounts of ZnMT from Cd,ZnMT.
Comeau
R D
RD
Seragen Inc., Hopkington, MA 01748.
McDonald
K W
KW
Tolman
G L
GL
Vasak
M
M
Liberatore
F A
FA
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008099:Liver / Q000737:chemistry*; D008668:Metallothionein / Q000302:isolation & purification*; D011817:Rabbits; D015032:Zinc / Q000302:isolation & purification*
C021267:zinc thionein; D008668:Metallothionein; D015032:Zinc