Identity of a differentiation inhibiting factor for mouse myeloid leukemia cells with NM23/nucleoside diphosphate kinase.
Mouse myeloid leukemic line M1 cells can be induced to differentiate into the monocyte/macrophage pathway by various inducers. The induction of differentiation of M1 cells can be inhibited by protein inhibitors termed differentiation inhibiting factors (I-factors) in a cell lysate and conditioned medium of differentiation resistant M1 cells. Production of the I-factor activity in resistant M1 cells is well associated with development of resistance of M1 cells to differentiation inducers. We have now purified one of the I-factors from conditioned medium of differentiation resistant M1 cells. The purified I-factor has a relative molecular mass of approximately 16000-17000 Da (16K I-factor). The amino acid sequence of all fragments of the 16K I-factor we have found are identical with Nm23/nucleoside diphosphate kinase (EC2.7.4.6) protein involved in tumor metastasis. The findings indicate that the I-factor, a candidate suppressor protein for differentiation of leukemic cells, is Nm23/nucleoside diphosphate kinase protein.
Okabe-Kado
J
J
Department of Chemotherapy, Saitama Cancer Center Research Institute, Japan.
Kasukabe
T
T
Honma
Y
Y
Hayashi
M
M
Henzel
W J
WJ
Hozumi
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D003907:Dexamethasone; D004591:Electrophoresis, Polyacrylamide Gel; D006131:Growth Inhibitors / Q000502:physiology; D006801:Humans; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D007942:Leukemia, Experimental; D008222:Lymphokines / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009701:Nucleoside-Diphosphate Kinase / Q000302:isolation & purification / Q000502:physiology*; D010446:Peptide Fragments / Q000302:isolation & purification; D010587:Phagocytosis; D012689:Sequence Homology, Nucleic Acid
D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D010446:Peptide Fragments; D003907:Dexamethasone; D009701:Nucleoside-Diphosphate Kinase