[The expert diagnosis of HIV infection by radioimmunoprecipitation].
Using a panel of sera from HIV-infected persons and donors, the authors showed that radioimmunoprecipitation assays compare favourably with immunoblotting assays. With radioimmunoprecipitation, cross reactions were observed between HIV-2 antigens and HIV-2 antibodies, and that the nature of cross reactivity differs from that observed with immunoblotting. Potentials of radioimmunoprecipitation assays as a confirmatory test for use with sera that have given indeterminate results in immunoblotting assays and contradictory results in enzyme immunoassays are examined.
Karamov
E V
EV
Lukashov
V V
VV
Slepushkin
V A
VA
Chaplinskas
S A
SA
Petkov
V
V
Vojcehovský
K
K
Kopecká
D
D
Gorbacheva
A P
AP
Apuokene
E
E
Brucková
M
M
D015492:AIDS Serodiagnosis / Q000379:methods*; D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis; D003429:Cross Reactions; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D015483:HIV Antibodies / Q000097:blood; D015488:HIV Antigens / Q000097:blood; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology; D015498:HIV-2 / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D015531:Radioimmunoprecipitation Assay / Q000379:methods*; D012680:Sensitivity and Specificity
D015483:HIV Antibodies; D015488:HIV Antigens
Ekspertnaia diagnostika VICh-infektsii metodom radioimmunopretsipitatsii.