Sequence analysis of M2 mRNA of bovine respiratory syncytial virus obtained from an F-M2 dicistronic mRNA suggests structural homology with that of human respiratory syncytial virus.
The nucleotide sequences of the F and M2 mRNAs of strain A51908 of bovine respiratory syncytial virus (BRSV) were determined by sequencing cDNA of an intracellular dicistronic mRNA. Comparison of the F mRNA sequence with those of other BRSV strains showed that there was extensive sequence identity at both the nucleotide (95% identity) and amino acid (94% identity) levels. Alignment of the nucleotide and encoded amino acid sequences of M2 mRNA of BRSV with those of human respiratory syncytial virus (HRSV) M2 mRNA showed 69% identity at the nucleotide level and 80% identity at the amino acid level. The general features of BRSV F and M2 proteins are similar to those described previously for the HRSV proteins. The M2 mRNA of BRSV also contained a second internal, overlapping open reading frame (ORF) similar to one reported for HRSV. The predicted products of the second ORFs of BRSV and HRSV shared 43% amino acid identity. As described for HRSV, the 3'-terminal end of the M2 mRNA overlaps with the 5' end of the L gene by 68 nucleotides. The identity between the N-terminal regions of the L proteins of BRSV and HRSV is 75%. In addition, the intergenic sequence of the F-M2 gene junction of BRSV was determined.
Zamora
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Regional College of Veterinary Medicine, University of Maryland, College Park 20742.
Samal
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D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D015707:HN Protein; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D016364:Reading Frames; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D012192:Retroviridae Infections / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D016092:Spumavirus / Q000235:genetics*; D014759:Viral Envelope Proteins; D014764:Viral Proteins
D000956:Antigens, Viral; D015707:HN Protein; D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G