[The electrophoresis of biotinylated nucleic acids].
A simple and rapid method was worked out to evaluate the biotinylation level of the pBR322 and pSVK1H genetic cloning vectors, using gel electrophoresis. Avidin was used to slow down the migration of biotinylated DNA: the DNA migration speed diminished as the biotinylation level rose, due to DNA complexation. The highest level of biotinylation is characterized by the formation of a biotinylated nucleic acid-avidin complex with no electrophoretical mobility.
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Institut de Virologie Stefan S. Nicolau, Bucarest, Roumanie.
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D001360:Avidin; D001710:Biotin; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005822:Genetic Vectors; D007202:Indicators and Reagents; D009696:Nucleic Acids / Q000032:analysis*; D010957:Plasmids / Q000032:analysis
D007202:Indicators and Reagents; D009696:Nucleic Acids; D001360:Avidin; D001710:Biotin; D004247:DNA
Electrophorèse des acides nucléiques biotinylés.