title: Alterations in quantitative distribution of Na,K-ATPase activity along crypt-villus axis in animal model of malabsorption characterized by hyperproliferative crypt cytokinetics.
abstract: The distribution of sodium- and potassium-stimulated ATPase (Na,K-ATPase) along the crypt-villus axis and crypt cytokinetics were examined in an infective model of celiac disease produced by infection of the rat with the nematode Nippostrongylus brasiliensis. In controls, levels of enzyme activity remained stable during enterocyte migration to the villous apex. In the jejunum of infected rats, the structural lesion of villous atrophy and crypt hyperplasia, observed at day 10 of infection, was associated with a three-dimensional expansion of the crypts. Cell cycle time was shortened and this resulted in a markedly increased crypt cell production rate. Enterocytes emerged from the crypts at a faster rate, and this functional immaturity was paralleled by decreased Na,K-ATPase activity. Further decreases in enzyme levels were observed during enterocyte migration along the villi. This may reflect enterocyte damage or increased enzyme turnover. In the ileum of these animals, enterocyte maturation was prolonged and enzyme activity was increased at the level of the crypt villus junction with further increases noted during enterocyte transit. These changes in ileal Na,K-ATPase appear to be adaptive.
authors.affiliation: Department of Medicine, McGill University, Montreal, Canada.
authors.forename: G E
authors.initials: GE
authors.lastname: Wild
authors.forename: D
authors.initials: D
authors.lastname: Murray
mesh_terms: D000818:Animals; D002446:Celiac Disease / Q000201:enzymology*; D004195:Disease Models, Animal; D005772:Gastrointestinal Transit / Q000502:physiology; D006965:Hyperplasia; D007082:Ileum / Q000201:enzymology / Q000473:pathology; D007583:Jejunum / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008940:Mitotic Index / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D000254:Sodium-Potassium-Exchanging ATPase