Rearrangement and co-amplification of L-myc and rlf in primary lung cancer.
We have recently characterized a gene fusion and chimeric protein product formed by L-myc and part of a novel gene named rlf in two small-cell lung cancer (SCLC) cell lines. The rlf-L-myc fusion gene is formed by intrachromosomal rearrangements placing the regulatory region and (at least) the first exon of rlf upstream of the L-myc gene. In the characterized cases the fusion gene has also been involved in DNA amplification. Here we report on a similar in vivo rearrangement involving rlf and L-myc in a primary SCLC tumor. In addition, we have found co-amplification of L-myc and rlf without visible rearrangements in either gene in three other SCLC tumors, confirming the physical linkage of these loci.
Mäkelä
T P
TP
Department of Virology, University of Helsinki, Finland.
Shiraishi
M
M
Borrello
M G
MG
Sekiya
T
T
Alitalo
K
K
D015139:Blotting, Southern; D018288:Carcinoma, Small Cell / Q000235:genetics*; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D015514:Oncogene Proteins, Fusion; D009857:Oncogenes; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D015183:Restriction Mapping; D014760:Viral Fusion Proteins / Q000235:genetics*
D004273:DNA, Neoplasm; D015514:Oncogene Proteins, Fusion; D016271:Proto-Oncogene Proteins c-myc; D014760:Viral Fusion Proteins; C073593:rlf-L-myc fusion protein, human