Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens.
The present report describes an alternative method for in vitro detection of HIV-1-specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting HIV-1 infection early after birth. There were neither false-positivity nor false-negativity when blood samples obtained from HIV-1 seronegative asymptomatic individuals, and HIV-1 seropositive adult patients were analyzed. This rapid, low cost, simple, highly sensitive and specific assay can be extremely useful for early diagnosis of pediatric HIV infection.
Caterino-de-Araujo
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Seção de Imunologia, Instituto Adolfo Lutz, SP, Brasil.
D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015483:HIV Antibodies / Q000276:immunology / Q000302:isolation & purification*; D015488:HIV Antigens / Q000276:immunology; D006679:HIV Seropositivity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007223:Infant; D008875:Middle Aged; D011247:Pregnancy; D012680:Sensitivity and Specificity; D013997:Time Factors
D015483:HIV Antibodies; D015488:HIV Antigens