[The immunologic studies on experimental heterogeneous penetrating keratoplasty].
Using the experimental model of chicken-rabbit penetrating corneal grafting, micro-whole-blood 3H-TdR incorporation and ELISA the authors dynamically observed the functional state of cellular and humoral immunities for 10 weeks after operation. The results showed, (1) In both the homogroup and the hetero-group, the transformational degree of lymphocytes in peripheral blood increased, most obviously in the 3rd or 4th week postoperatively. (2) In the hetero-group, specific anti-corneal antibody appeared obviously in the serum during 2-8 weeks postoperatively. Combined with morphologic observation, it has proved that: (1) The immune rejection is mainly cellular mediated immunoresponse, meanwhile, the humoral immunoresponse also participates in it, and the humoral immunoresponse participates in the heterokeratoplasty more obviously. (2) Applying the micro-whole-blood 3H-TdR incorporation is capable of determining the immunologic state of the body after corneal transplantation. (3) Attention should be focused on the 3-4 week period postoperatively when immunosuppressive-agent is administered.
Zhong
J
J
Department of Ophthalmology, Medical College of Jinan University, Guangzhou, China.
Xu
J
J
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002645:Chickens; D003315:Cornea / Q000276:immunology* / Q000473:pathology; D003907:Dexamethasone / Q000494:pharmacology; D006789:Host vs Graft Reaction / Q000187:drug effects / Q000276:immunology*; D015948:Keratoplasty, Penetrating / Q000276:immunology*; D008213:Lymphocyte Activation; D015166:Monitoring, Immunologic; D011817:Rabbits; D014183:Transplantation, Heterologous / Q000276:immunology*
D000906:Antibodies; D003907:Dexamethasone