Comparison of cryopreparation techniques for electron probe microanalysis of cells as exemplified by human erythrocytes.
Erythrocytes in human blood were used to evaluate the reliability of cryopreparation techniques for electron probe X-ray microanalysis of biological cells and tissues. The elemental content determined by X-ray microanalysis of ultrathin freeze-dried cryosections was found to be consistent with data known from the literature. Considerable redistribution of the intracellular elemental composition was found after freeze-substitution as well as after freeze-drying followed by resin embedding. Two conclusions are drawn from this study: 1. Erythrocytes in human blood are a suitable reference specimen for evaluation of specimen preparation techniques for microanalysis. 2. At present, freeze-dried cryosections are the most reliable specimen type for quantitative electron probe microanalysis of cells.
Zierold
K
K
Max-Planck-Institut für Systemphysiologie, Dortmund, Federal Republic of Germany.
D015925:Cryopreservation / Q000379:methods*; D004577:Electron Probe Microanalysis; D004912:Erythrocytes; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity