Cloning and sequencing of human kappa-casein cDNA.
A cDNA encoding kappa-casein of human milk was cloned and sequenced. The kappa-casein cDNA was isolated from a lambda gt11 library generated from mRNA prepared from a mammary gland biopsy obtained from a lactating woman. The library was screened with polyclonal rabbit antibodies raised against purified native kappa-casein. The obtained nucleotide sequence contained an ORF sufficient to encode the entire amino acid sequence of a kappa-casein precursor protein consisting of 182 amino acids. This includes a tentative signal peptide of 20 amino acids and a processed protein of 162 amino acids.
Bergström
S
S
Department of Microbiology, University of Umeå, Sweden.
Hansson
L
L
Hernell
O
O
Lönnerdal
B
B
Nilsson
A K
AK
Strömqvist
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
D002364:Caseins; D012333:RNA, Messenger; D004247:DNA