[Studies on human cervical carcinoma cell line. II. Establishment of HGPRT- cell line CC-80IAR2 and its biological characteristics].
An HGPRT- cell line, CC-801AR2, was established from cloned human cervical carcinoma cell line CC-801 by using MNNG to induce cell mutation and 8-azaguanine (8-AG) to select the 8-AG resistant cells. The deficiency of HG-PRT in CC-801AR2 cells was proved by enzyme activity assay. This cell line was very sensitive to HAT medium. It was very similar in biological characteristics to CC-801, especially in that it retained its tumorigenicity when transplanted into nude mice. CC-801AR2 can therefore be used in somatic cell hybridization and gene transfer experiments.
Wang
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Institute of Basic Medical Sciences, Beijing.
D000782:Aneuploidy; D000818:Animals; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine; D051379:Mice; D008819:Mice, Nude; D008940:Mitotic Index; D009154:Mutation; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured / Q000187:drug effects; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*
D004273:DNA, Neoplasm; D008769:Methylnitronitrosoguanidine; D007041:Hypoxanthine Phosphoribosyltransferase