title: Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparison of the assay with ELISA.
abstract: The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.
authors.affiliation: Serology Section, Instituto Adolfo Lutz, São Paulo, Brasil.
authors.forename: E D
authors.initials: ED
authors.lastname: Camargo
authors.forename: P M
authors.initials: PM
authors.lastname: Nakamura
authors.forename: A J
authors.initials: AJ
authors.lastname: Vaz
authors.forename: M V
authors.initials: MV
authors.lastname: da Silva
authors.forename: P P
authors.initials: PP
authors.lastname: Chieffi
authors.forename: E O
authors.initials: EO
authors.lastname: de Melo
mesh_terms: D000293:Adolescent; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012680:Sensitivity and Specificity; D014120:Toxocariasis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000947:Antigens, Helminth