[Comparative studies on LH/CG receptor from rat testes and hCG-binding protein from Pseudomonas maltophilia by antibodies against the hCG-binding protein].
An indirect immunoprecipitation experiment was developed to characterize the antigenic determinants of the hCG-binding protein and the rat testicular LH/CG receptor. The hCG-binding protein preincubated with 125I iodo hCG was immunologically precipitated by the antibodies against the hCG-binding protein in the presence of goat anti-rabbit IgG. Similarly, the LH/CG receptor solubilized with 1% triton X-100 from the rat testes was also immunoprecipitated by the antibodies. Furthermore, the antibodies did not inhibit the binding of 125I iodo hCG to the hCG-binding protein or to the solubilized rat testicular LH/CG receptor. From these results it can be concluded that there exists the sharing of antigenic determinants between the hCG-binding protein from Pseudomonas maltophilia and the LH/CG receptor from the rat testes and that the antibodies specifically recognize the antigenic determinants outside the hCG-binding sites on the rat testicular LH/CG receptor. Therefore, the antibodies against the hCG-binding protein are of potential applications to studies on structure and function of the mammalian LH/CG receptors.
Li
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Chen
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Lan
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D000818:Animals; D002352:Carrier Proteins / Q000276:immunology*; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D000939:Epitopes; D008297:Male; D011233:Precipitin Tests; D011549:Pseudomonas / Q000378:metabolism; D051381:Rats; D011974:Receptors, LH / Q000276:immunology*; D013737:Testis / Q000378:metabolism*
D002352:Carrier Proteins; D006063:Chorionic Gonadotropin; D000939:Epitopes; D011974:Receptors, LH