Characterization of sulphoalkyl ether derivatives of beta-cyclodextrin by capillary electrophoresis with indirect UV detection.
A capillary electrophoresis method which characterizes the degrees of substitution of heterogeneous sulphoalkyl ether beta-cyclodextrin derivatives is described. The separation is based on the different electrophoretic mobilities observed from changes in the overall charge of the molecule as a result of substitution. Individual peaks of the electropherogram then provide a measure of each degree of substitution of the present beta-cyclodextrin. Detection of these beta-cyclodextrin derivatives is performed by indirect UV detection.
Tait
R J
RJ
Center for Drug Delivery Research, University of Kansas, Lawrence 66045.
Skanchy
D J
DJ
Thompson
D P
DP
Chetwyn
N C
NC
Dunshee
D A
DA
Rajewski
R A
RA
Stella
V J
VJ
Stobaugh
J F
JF
D001565:Benzoates / Q000737:chemistry; D019817:Benzoic Acid; D003505:Cyclodextrins / Q000138:chemical synthesis* / Q000737:chemistry*; D004586:Electrophoresis; D013056:Spectrophotometry, Ultraviolet; D047392:beta-Cyclodextrins
D001565:Benzoates; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D019817:Benzoic Acid; C031215:betadex