Origin of sequence specific cleavage of DNA by bleomycins.
A new DNA photocleaving agent which contains bleomycin A2's DNA binding portion and its mono- and terthiazole analogues have been designed and synthesized, and their DNA binding mode and cleavage base specificity have been studied. The photoactive p-nitrobenzoyl group attached at the end of molecules cleaves DNA on UV irradiation. All the oligo-thiazole compounds exhibited high sequence specificity in DNA scission. The bithiazole derivative did not cleave DNA at or near 5'-GpT-3' or 5'-GpC-3' as expected from widely believed DNA binding mechanism of the antibiotics.
Kuroda
R
R
Department of Chemistry, College of Arts and Sciences, University of Tokyo, Japan.
Shinomiya
M
M
Otsuka
M
M
D001761:Bleomycin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D010777:Photochemistry; D014466:Ultraviolet Rays
D001761:Bleomycin; D004247:DNA