title: Rapid generation of region specific probes by chromosome microdissection and their application.
abstract: The strategy presented here to identify unequivocally cryptic chromosomal rearrangements has relevance to both prenatal and postnatal cytogenetic analysis as well as the analysis of tumour-associated chromosome rearrangements. Microdissection and in vitro amplification of specific chromosomal regions are performed, followed by labelling for fluorescent in situ hybridization (FISH) to normal metaphase chromosomes (Micro-FISH). Micro-FISH probes have been used successfully to determine the derivation of chromosome segments unidentifiable by standard chromosome banding analysis. Micro-FISH probes (created in less than 24 hours) now make it possible to identify explicitly the chromosome constitution of virtually all cytologically visible chromosome rearrangements.
authors.affiliation: Department of Radiation Oncology, University of Michigan, Ann Arbor 48109-0668.
authors.forename: P S
authors.initials: PS
authors.lastname: Meltzer
authors.forename: X Y
authors.initials: XY
authors.lastname: Guan
authors.forename: A
authors.initials: A
authors.lastname: Burgess
authors.forename: J M
authors.initials: JM
authors.lastname: Trent
mesh_terms: D001483:Base Sequence; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002877:Chromosomes, Human / Q000648:ultrastructure*; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis; D014178:Translocation, Genetic
chemical_list: D015342:DNA Probes; D004247:DNA