[Effect of hyperthermia and retinol treatment in vitro on HTC cell adhesiveness to laminin and fibronectin].
Since cell adhesiveness is very important in the metastatic process and because both hyperthermia and treatment with Retinol can modify the fluidity of the lipid components of the plasma membrane (and therefore its receptor distribution), we investigated if a hyperthermic treatment (at 42 degrees C or 44 degrees C, for one hour) of HTC hepatoma cells, preceded or followed by treatment with 5 microM Retinol, could alter cell adhesiveness to Laminin or to Fibronectin-coated substrata. Hepatoma cells, after such treatments, were collected and processed by Auerbach's method. In the control cells thermal treatment alone caused a decrease of adhesiveness to Laminin but no change in that to Fibronectin. When treatment with Retinol was carried out before hyperthermia, the cells showed an increased adhesiveness to Laminin and a decreased adhesiveness to Fibronectin. Instead, when treatment with Retinol was performed in cells previously thermo-selected, a decrease of adhesiveness to both tested ligands was observed.
Càllari
D
D
Istituto di Patologia Generale, Università di Catania.
Blandino
G
G
Saccone
V
V
D'Amico
C
C
Billitteri
A
A
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D005353:Fibronectins / Q000378:metabolism*; D006358:Hot Temperature; D007797:Laminin / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D008560:Membrane Fluidity / Q000187:drug effects; D009363:Neoplasm Proteins / Q000378:metabolism*; D051381:Rats; D017469:Receptors, Fibronectin / Q000378:metabolism*; D017474:Receptors, Laminin / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014801:Vitamin A / Q000494:pharmacology*
D005353:Fibronectins; D007797:Laminin; D009363:Neoplasm Proteins; D017469:Receptors, Fibronectin; D017474:Receptors, Laminin; D014801:Vitamin A; D000431:Ethanol
Azione dell'ipertermia e del trattamento con retinolo in vitro sull'adesivita' alla laminina ed alla fibronectina delle cellule HTC.