title: A comparative study of hemocytes from six different snails: morphology and functional aspects.
abstract: Hemocytes taken from six different gastropod snails, Achatina achatina, A. fulica, Biomphalaria glabrata, Bulinus natalensis, Helix aspersa, and Lymnaea stagnalis, were compared for morphology, peroxidase activity, and, using methods developed for L. stagnalis, the ability to generate reactive oxygen inermediates upon phagocytic stimulation. Numbers of hemocytes per milliliter hemolymph and hemocytes' microscopical morphology showed some variation among the snail species. Peroxidase activity was demonstrated in all snail hemocytes except in those of B. glabrata and A. fulica. Hemocytes of all species generated superoxide upon phagocytic stimulation with zymosan (tested by superoxide dismutase-inhibitable reduction of nitroblue tetrazolium). When tested, hemocytes of A. achatina and of A. fulica displayed luminol-dependent chemiluminescence activity.
authors.affiliation: Faculty of Medicine, Vrije Universiteit Amsterdam, The Netherlands.
authors.forename: C M
authors.initials: CM
authors.lastname: Adema
authors.forename: R A
authors.initials: RA
authors.lastname: Harris
authors.forename: E C
authors.initials: EC
authors.lastname: van Deutekom-Mulder
mesh_terms: D000818:Animals; D002452:Cell Count; D006434:Hemocytes / Q000166:cytology* / Q000201:enzymology / Q000502:physiology; D009195:Peroxidase / Q000032:analysis; D012908:Snails / Q000166:cytology*; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides; D009195:Peroxidase