[Optimization of culture conditions for the production of Salmonella typhi O and H antigenic suspensions].
A Behring's production protocol of antigenic suspensions O and H of Salmonella typhi has been studied. The object of this study has been the Search of optimal conditions of cultivation and their exploitation on fermentor. A fermentation using the I.P.A culture medium has been realized to compare its results with that obtained using Behring's culture medium (B.C.M). The optimal cultivation conditions obtained are a waving rate of 400 tr/mn, a pH of 7.6 and an important air flow. Relating to nutritious constituents, the adequate glucose concentration is about 8g/1 and it seems better to replace meat extract by yeast extract. Comparatively to I.P.A culture medium, one of the most important advantage of Behring's culture medium modified reside in the conservation of the antigenicity of Salmonella typhi.
Amrouche
M
M
Stagiaires Service Vaccins Bactériens-Institut Pasteur d'Algérie.
Seddi
R
R
Kadra
B
B
Bakour
R
R
D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000302:isolation & purification; D001431:Bacteriological Techniques; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D019081:O Antigens; D010100:Oxygen; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000302:isolation & purification; D012485:Salmonella typhi / Q000254:growth & development* / Q000276:immunology
D000942:Antigens, Bacterial; D003470:Culture Media; C022863:H antigen; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D010100:Oxygen
Optimisation des conditions de culture pour la production de suspensions antigéniques O et H de Salmonella typhi.