A new continuous optical assay for maltase and sucrase.
A new method for the assay of maltase and sucrase is reported. The method makes use of mutarotase, hexokinase and glucose 6-phosphate dehydrogenase as ancillary enzymes. The reaction is linear at least up to a delta E/min of 0.13.
Giorgi
M
M
Dipartimento di Scienze Biochimiche, Firenze, Italia.
Vanni
P
P
Pinzauti
G
G
D002462:Cell Membrane / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase; D006098:Granulocytes / Q000201:enzymology; D006593:Hexokinase; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013393:Sucrase / Q000032:analysis* / Q000097:blood; D000520:alpha-Glucosidases / Q000032:analysis* / Q000097:blood
D007202:Indicators and Reagents; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D000520:alpha-Glucosidases; D013393:Sucrase