Enhanced sensitivity to oxidative stress in Cu,ZnSOD depleted rat erythrocytes.
The effects on red blood cells of superoxide dismutase (Cu,ZnSOD) depletion, induced by feeding Wistar rats with a copper deficient diet, were investigated. SOD depleted red blood cells were more sensitive to peroxidation and to hemolysis than normal cells when exposed to tert-butylhydroperoxide (t-BOOH). Membranes isolated from SOD depleted cells showed a lower content of vitamin E and higher (Na+, K+) and Mg2+ ATPase activities. These results support the view that superoxide dismutase plays an important role in cellular oxidative metabolism.
Bartoli
G M
GM
Department of Biology, University of Tor Vergata, Rome, Italy.
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D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D006461:Hemolysis; D007700:Kinetics; D015227:Lipid Peroxidation; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood*; D014810:Vitamin E / Q000097:blood
D014810:Vitamin E; D013482:Superoxide Dismutase; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase