Molecular evolution of the human interleukin-8 receptor gene cluster.
Interleukin-8 (IL-8) is the prototype for a family of at least eight neutrophil chemoattractants whose genes map to human chromosome 4q13-q21. Two human IL-8 receptors, IL8RA and IL8RB, are known from cDNA cloning; IL8RA is a promiscuous receptor for at least two other related ligands, GRO alpha and NAP-2. We now report cloning of the genes for IL8RA, IL8RB and a recently inactivated pseudogene of receptor A (IL8RAP). These form a cluster of only three genes in the superfamily of G protein-coupled receptors (GPCRs) and map to 2q34-q35. The coevolutionary diversity displayed by the IL-8 ligand-receptor complex--ligand promiscuity for IL-8, receptor promiscuity for IL8RA, gene duplication for both ligands and receptors and gene extinction in the case of IL8RAP--is unprecedented for the GPCR superfamily.
Ahuja
S K
SK
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Ozçelik
T
T
Milatovitch
A
A
Francke
U
U
Murphy
P M
PM
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D004247:DNA / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011544:Pseudogenes; D011971:Receptors, Immunologic / Q000235:genetics*; D023062:Receptors, Interleukin-8A; D017386:Sequence Homology, Amino Acid
D011971:Receptors, Immunologic; D023062:Receptors, Interleukin-8A; D004247:DNA