Recombinant antigens in viral diagnosis.
DNA fragments coding for a variety of different viral antigens have been cloned and expressed in Escherichia coli. Selected purified recombinant antigens were used for detection of specific antibodies by means of the ELISA technique. This approach has been used for the development of four different ELISAs for the detection of HIV- and EBV-specific antibodies.
Vornhagen
R
R
Biotest AG, Research Department, Dreieichenhain, FRG.
Baur
A
A
Jahn
G
G
Hinderer
W
W
Nebel-Schickel
H
H
Horn
J
J
Wolf
H
H
Sonneborn
H H
HH
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D015483:HIV Antibodies / Q000032:analysis*; D015497:HIV-1 / Q000276:immunology; D015498:HIV-2 / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D011994:Recombinant Proteins
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D015483:HIV Antibodies; D011994:Recombinant Proteins