Interactions between anticollagen antibodies and chondrocytes.
OBJECTIVE
To investigate the interactions between anticollagen antibodies and living chondrocytes.


METHODS
Mouse monoclonal anti-type II collagen (anti-CII) antibodies, rabbit anti-human CII, and rat anti-CII, anti-CIV, anti-CV, anti-CVI, and anti-CIX were studied in vitro to determine their ability to bind to the plasma membrane of living bovine chondrocytes.


RESULTS
Mouse monoclonal anti-CII, rabbit anti-CII, and rat anti-CII, anti-CV, and anti-CIX were shown to bind in vitro to the plasma membrane of bovine chondrocytes. Antibody binding was not observed with anti-CIV, or with chondrocytes previously incubated with bacterial collagenase. A significant increase in chondrocyte caseinase and collagenase secretion was observed following sequential incubation with the monoclonal antibodies and a source of activating cytokines.


CONCLUSIONS
These results suggest that collagen autoantibodies may exert some of their pathogenic effects on cartilage through interactions with resident chondrocytes, leading to modulation of the rate of secretion of cartilage matrix-degrading enzymes.
Takagi
T
T
Department of Internal Medicine, University of Texas Southwestern Medical School, Dallas.
Jasin
H E
HE
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D002358:Cartilage, Articular / Q000166:cytology* / Q000201:enzymology / Q000276:immunology*; D002417:Cattle; D002462:Cell Membrane / Q000276:immunology; D003094:Collagen / Q000276:immunology*; D005434:Flow Cytometry; D008666:Metalloendopeptidases; D003012:Microbial Collagenase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D011817:Rabbits; D051381:Rats
D000906:Antibodies; D003094:Collagen; D010447:Peptide Hydrolases; D008666:Metalloendopeptidases; C030895:caseinase; D003012:Microbial Collagenase