title: Freezing technique and quality of fresh-frozen plasma.
abstract: Cell-poor plasma was prepared by apheresis from 10 donors. From each donor, an amount of 200 ml was frozen rapidly to -40 degrees C in standard blood bags, and a further 200 ml was frozen slowly to -20 degrees C. Before freezing and after thawing, plasma samples were collected and frozen to -70 degrees C pending analysis. Coagulation factor VIII activity was reduced to 90% by rapid freezing and to 80% by slow freezing. Factor V was not influenced by rapid freezing, but slow freezing reduced the levels to 92% of the pre-freezing levels. In some of the plasma bags a slight increase in fibrinopeptide A occurred. However, soluble fibrin, thrombin-antithrombin complexes and spontaneous proteolytic activity were not altered by freezing. The beta-thromboglobulin increased slightly with slow freezing. Moreover, in a separate experiment, evaluating the possible effects of refreezing plasma samples, an increase in beta-thromboglobulin was also recorded, while the levels of factors VIII and V and von Willebrand factor were not affected. The changes in some variables, which were recorded in the cell-poor plasma, frozen soon after the blood donation at a slow freezing rate, must be regarded as insignificant in most clinical situations.
authors.affiliation: Klinisk Immunologi och Transfusionsmedicin och, Karolinska Sjukhuset, Stockholm, Sverige.
authors.forename: O
authors.initials: O
authors.lastname: Akerblom
authors.forename: K
authors.initials: K
authors.lastname: Bremme
authors.forename: A L
authors.initials: AL
authors.lastname: Dackland
authors.forename: K
authors.initials: K
authors.lastname: Fatah
authors.forename: A M
authors.initials: AM
authors.lastname: Suontaka
authors.forename: M
authors.initials: M
authors.lastname: Blombäck
mesh_terms: D000990:Antithrombin III / Q000032:analysis; D001779:Blood Coagulation Factors / Q000032:analysis; D015925:Cryopreservation / Q000379:methods*; D005337:Fibrin / Q000032:analysis; D005344:Fibrinopeptide A / Q000032:analysis; D006487:Hemostasis / Q000502:physiology; D006801:Humans; D010447:Peptide Hydrolases / Q000032:analysis; D010949:Plasma; D011786:Quality Control; D001620:beta-Thromboglobulin / Q000032:analysis; D014841:von Willebrand Factor / Q000032:analysis
chemical_list: D001779:Blood Coagulation Factors; C046193:antithrombin III-protease complex; D001620:beta-Thromboglobulin; D014841:von Willebrand Factor; D005344:Fibrinopeptide A; D000990:Antithrombin III; D005337:Fibrin; D010447:Peptide Hydrolases