Urinary cholinesterase activity is increased in insulin-dependent diabetics: further evidence of diabetic tubular dysfunction.
We measured the cholinesterase activity in morning urines from 63 insulin-dependent diabetics and 27 controls. The total esterase (TotE) activity (Ellman's method) has been divided into aliesterase (AliE), pseudocholinesterase and acetylcholinesterase by means of two inhibitors, eserine and quinidine. Diabetics were divided in 2 groups according to the urinary albumin/creatinine ratio (mg/mmol, < 2 in group 1, > 2 in group 2). The urinary cholinesterase behavior was correlated with that of a known tubular lysosomal hydrolase, N-acetyl-beta-D-glucosaminidase (NAG). Compared to normals, in addition to a significant increase in urinary NAG in diabetes (in group 2 more than in group 1), TotE and AliE were also significantly raised (+36% and 109% of the controls, in group 1 as much as in group 2).
Matteucci
E
E
Institute of Clinical Medicine II, Postgraduate Medical School of Clinical Pathology, University of Pisa, Italy.
Pellegrini
L
L
Uncini-Manganelli
C
C
Cecere
M
M
Saviozzi
M
M
Giampietro
O
O
D000293:Adolescent; D000328:Adult; D000419:Albuminuria; D015415:Biomarkers / Q000652:urine; D001786:Blood Glucose / Q000032:analysis; D002802:Cholinesterases / Q000652:urine*; D003404:Creatinine / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000201:enzymology / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology / Q000652:urine*; D003928:Diabetic Nephropathies / Q000097:blood / Q000201:enzymology / Q000652:urine*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006029:Glycosuria; D006801:Humans; D007684:Kidney Tubules / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D012016:Reference Values
D015415:Biomarkers; D001786:Blood Glucose; D002802:Cholinesterases; D003404:Creatinine; D006442:Glycated Hemoglobin