Murine monoclonal antibodies against pneumococcal capsular polysaccharide types 4, 8, 22F and 19A/19F.
Monoclonal antibodies (MAbs) were produced against pneumococcal capsular polysaccharides after subcutaneous immunization of BALB/c mice with a 23-valent vaccine (Pneumovax N, Merck, Sharp & Dohme). Selected antibodies were tested in ELISA against individual polysaccharides from 23 different pneumococcal types and in a dot blot assay with heat-killed whole bacteria adhered to nitrocellulose paper. Three MAbs (isotype IgM) were found to be specific for types 4, 8 and 22F, respectively, whereas one (isotype IgA) reacted both with 19A and 19F. Very mild acid hydrolysis of the capsular polysaccharides resulted in loss of reaction with the antibodies.
Kolberg
J
J
Department of Immunology, National Institute of Public Health, Oslo, Norway.
Aaberge
I S
IS
Jantzen
E
E
Løvik
M
M
Lermark
G
G
Steen
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D051379:Mice; D010504:Periodic Acid / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001428:Bacterial Vaccines; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid