title: [Cloning and expression of the Clostridium thermocellum gene in cyanobacteria Anacystis nidulans R2 cells].
abstract: The XhoI-SalGI fragment of the plasmid pCI DNA was inserted into the SalGI site of the cyanobacterium Anacystis nidulans R2 integrative vector plasmid pIAH4. The fragment incorporates the endoglucanase gene of Clostridium thermocellum cloned earlier within the 6.7 kb DNA sequence. The recombinant plasmid DNA was transformed into Anacystis nidulans R2 cells. The cloned endoglucanase gene was shown to express in the cyanobacterium cells. The enzyme synthesized is accumulated within the cytoplasm of Anacystis nidulans cells and is not secreted into the periplasm.
authors.forename: R L
authors.initials: RL
authors.lastname: Vil'chinkas
authors.forename: I V
authors.initials: IV
authors.lastname: Elanskaia
mesh_terms: D002480:Cellulase / Q000235:genetics*; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology*; D000458:Cyanobacteria / Q000235:genetics*; D004269:DNA, Bacterial; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial; D002480:Cellulase
vernacular_title: Klonirovanie i ékspressiia gena éndogliukanazy Clostridium thermocellum v kletkakh tsianobakterii Anacystis nidulans R2.