[Changes of the insulin and glucagon receptors in bile-duct ligated rats].
The specific bindings of insulin and glucagon to 40000g pellets including hepatic plasma membrane were measured at 1, 2 or 4 weeks after bile-duct ligation or sham operation in rats. At 1 week after operation, insulin bindings were similar in both bile-duct ligated and control rats, however, glucagon binding in bile-duct ligated rats (30.1 +/- 5.99%) was significantly (p less than 0.01) lower than that in control rats (42.8 +/- 8.13%). At 2 and 4 weeks after operation, insulin bindings in bile-duct ligated rats (2 weeks: 35.2 +/- 3.32%, 4 weeks: 32.3 +/- 4.62%) were significantly (p less than 0.05) lower than those in control rats (2 weeks: 39.7 +/- 5.12%, 4 weeks: 38.4 +/- 3.85%). Therefore, glucagon bindings in bile-duct ligated rats (2 weeks: 26.3 +/- 4.32%, 4 weeks: 26.9 +/- 4.06%) were significantly (p less than 0.01) lower than those in control rats (2 weeks: 42.0 +/- 3.95%, 4 weeks: 45.3 +/- 4.29%). Decrease of insulin bindings, however, was less than that of glucagon bindings. Changes of the insulin and glucagon receptors on the hepatic plasma membrane suggested a reason for a disturbance of carbohydrate metabolism in the liver cell with obstructive jaundice.
Sakai
H
H
First Department of Surgery, Okayama University Medical School, Japan.
D000818:Animals; D001652:Bile Ducts / Q000601:surgery; D001665:Binding Sites; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000378:metabolism; D002779:Cholestasis / Q000378:metabolism*; D008026:Ligation; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000378:metabolism*; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism*; D018027:Receptors, Glucagon
D011964:Receptors, Gastrointestinal Hormone; D018027:Receptors, Glucagon; D011972:Receptor, Insulin