title: Examination of Koch's postulates for Borrelia burgdorferi as the causative agent of limb/joint dysfunction in dogs with borreliosis.
abstract: Borrelia burgdorferi has been implicated as the causative agent of borreliosis in dogs, which is characteristically a limb/joint disorder, but can be associated with multiple-organ dysfunction. Attempts to reproduce this disease by inoculating dogs with B burgdorferi have not been successful. In the study of this report, B burgdorferi from Ixodes dammini ticks was used to induce signs of limb/joint dysfunction, fever, anorexia, depression, and systemic infection in dogs. A pure culture of this bacterium from the blood of an infected dog has been used to fulfill Koch's postulates for B burgdorferi as the causative agent of limb/joint dysfunction associated with borreliosis in dogs.
authors.affiliation: Fort Dodge Laboratories, IA 50501.
authors.forename: T L
authors.initials: TL
authors.lastname: Wasmoen
authors.forename: R W
authors.initials: RW
authors.lastname: Sebring
authors.forename: B M
authors.initials: BM
authors.lastname: Blumer
authors.forename: L G
authors.initials: LG
authors.lastname: Chavez
authors.forename: H J
authors.initials: HJ
authors.lastname: Chu
authors.forename: W M
authors.initials: WM
authors.lastname: Acree
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000097:blood; D001096:Arachnid Vectors / Q000382:microbiology; D016470:Bacteremia / Q000382:microbiology / Q000662:veterinary*; D001483:Base Sequence; D015139:Blotting, Southern; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000097:blood / Q000737:chemistry; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D005334:Fever; D005455:Fluorescent Antibody Technique; D005938:Glucocorticoids; D015151:Immunoblotting; D007794:Lameness, Animal / Q000382:microbiology*; D008193:Lyme Disease / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013987:Ticks / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D005938:Glucocorticoids