title: Activation of protein kinase A increases the in vitro invasion, migration, and adherence to reconstituted basement membrane by Lewis lung carcinoma tumor cells.
abstract: Prostaglandin E2 (PGE2) stimulated in vitro invasion, migration, and adherence to reconstituted basement membrane by metastatic Lewis lung carcinoma (LLC) clones, but this stimulation was blocked by protein kinase A (PKA) inhibitors and by expression of a transfected mutant RI alpha which blocks PKA activation. In vitro migration and adherence of metastatic LLC transfectants was heightened by expression of a transfected C alpha gene and further heightened by Zn2+ induction of the expression vector. Nonmetastatic LLC were not migratory nor invasive. However, nonmetastatic LLC that were stably transfected with a C alpha gene were both migratory and invasive, particularly when C alpha expression was further induced with Zn2+. The results of these in vitro studies show that PKA can enhance the metastatic characteristics of LLC.
authors.affiliation: Research Service, Department of Veterans Affairs, Hines Hospital, Ill. 60141.
authors.forename: M R
authors.initials: MR
authors.lastname: Young
authors.forename: M E
authors.initials: ME
authors.lastname: Young
authors.forename: Y
authors.initials: Y
authors.lastname: Lozano
authors.forename: J M
authors.initials: JM
authors.lastname: Bagash
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D015232:Dinoprostone / Q000494:pharmacology; D004789:Enzyme Activation; D015870:Gene Expression / Q000502:physiology; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D051379:Mice; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D011494:Protein Kinases / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D014162:Transfection / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D011494:Protein Kinases; D015232:Dinoprostone