Improving accuracy of glucose oxidase procedure for glucose determinations on discrete analyzers.
We describe a rapid kinetic glucose oxidase (EC 1.1.3.4) procedure for quantifying glucose. Glucose oxidase concentration was reduced from the more usual 20 kU/L to 4 kU/L, and pH was reduced from 7.0 to 6.6. Potassium ferrocyanide (20 mumol/L) and ascorbate oxidase (1 kU/L) were incorporated in the procedure. The assay results vary linearly with glucose concentration from 0 to 50 mmol/L and are unaffected by bilirubin concentrations less than or equal to 600 mumol/L, hemoglobin less than or equal to 12 g/L, Intralipid less than or equal to 4 g/L, urate less than or equal to 1 mmol/L, and ascorbate less than or equal to 2.0 mmol/L. The assay is readily adaptable to most open-system analyzers.
Leary
N O
NO
Biochemistry Laboratory, Regional Hospital, Cork, Ireland.
Pembroke
A
A
Duggan
P F
PF
D001205:Ascorbic Acid; D001230:Aspergillus / Q000201:enzymology; D001322:Autoanalysis / Q000379:methods / Q000706:statistics & numerical data; D001663:Bilirubin / Q000097:blood; D001786:Blood Glucose / Q000032:analysis*; D005295:Ferrocyanides; D005949:Glucose Oxidase; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009195:Peroxidase; D011786:Quality Control
D001786:Blood Glucose; D005295:Ferrocyanides; D005949:Glucose Oxidase; D009195:Peroxidase; C031835:potassium ferrocyanide; D001205:Ascorbic Acid; D001663:Bilirubin