Assembly of 2-D membrane protein crystals: dynamics, crystal order, and fidelity of structure analysis by electron microscopy.
Membrane protein reconstitution into two-dimensional (2-D) ordered arrays is described. The assembly of 2-D crystals may be modeled as a two-step process: the membrane protein is first integrated in the lipid bilayer and then crystallized by removal of excess detergent or lipid and/or by precipitating agents. Lipid-detergent, protein-detergent, and lipid-protein interactions are critical during the first step, while lipid-protein and protein-protein interactions dominate events in the second step. The evidence supporting this model results from quasielastic light scattering analyses and electron microscopy of different lipid-detergent systems and reconstitution experiments with Escherichia coli porin OmpF, Phormidium laminosum photosystem I reaction centers, and integral membrane proteins of mammalian lens fiber cells.
Engel
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M.E. Müller-Institute for High-Resolution Electron Microscopy at the Biocenter, Univeristy of Basel, Switzerland.
Hoenger
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Hefti
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A
Henn
C
C
Ford
R C
RC
Kistler
J
J
Zulauf
M
M
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000648:ultrastructure; D003459:Crystallins / Q000737:chemistry / Q000648:ultrastructure; D003460:Crystallization; D003902:Detergents; D008055:Lipids; D008565:Membrane Proteins / Q000737:chemistry / Q000648:ultrastructure*; D008823:Micelles; D008854:Microscopy, Electron; D008958:Models, Molecular; D015394:Molecular Structure; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000648:ultrastructure; D045331:Photosystem I Protein Complex; D018272:Porins
D001425:Bacterial Outer Membrane Proteins; D003459:Crystallins; D003902:Detergents; D008055:Lipids; D008565:Membrane Proteins; D008823:Micelles; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D018272:Porins