title: [Problems encountered in identification of penicillin binding proteins with pneumococcus used as an example].
abstract: Whole cells or isolated membranes of Streptococcus pneumoniae were treated with labelled benzyl penicillin and the penicillin binding proteins (PBPs) were visualized by fluorography after SDS-PAGE electrophoresis. The PBP profiles obtained for strains sensitive and resistant to penicillin strongly differed depending not only on the concentrations of acrylamide and bis-acrylamide used in the separating gel but also on the batch used (different manufacturers). The latter was also true for sodium dodecyl sulfate.
authors.affiliation: Zakład Fizjologii Bakterii Instytutu Mikrobiologii Uniwersytetu Warszawskiego.
authors.forename: Z
authors.initials: Z
authors.lastname: Markiewicz
mesh_terms: D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000032:analysis*; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase / Q000032:analysis*; D010403:Penicillin Resistance; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000493:pharmacokinetics*; D010458:Peptidyl Transferases; D013296:Streptococcus pneumoniae / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase
vernacular_title: Problemy zwiazane z identyfikacja bakteryjnych białek wiazacach penicyline na przykładzie dwoinki zapalenia płuc.