On the anomalous behaviour on gel-filtration and SDS-electrophoresis of prothymosin-alpha.
The regulator of T-cell proliferation prothymosin alpha, is a protein with a relative molecular mass of 12 KDa as calculated from its amino acid sequence. This immunoregulator exhibits anomalous behaviour on gel-filtration and SDS-PAGE electrophoresis appearing as oligomers which are 5 or 2 fold larger than the corresponding polypeptide. These results suggest that a dimeric form of prothymosin alpha is stable to dissociation by SDS and reduction by beta-mercapto ethanol.
Cordero
O J
OJ
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Santiago, Santiago de Compostela, Spain.
Sarandeses
C S
CS
Lopez
J L
JL
Nogueira
M
M
D000328:Adult; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D010835:Phytohemagglutinins; D011498:Protein Precursors / Q000737:chemistry* / Q000494:pharmacology; D013947:Thymosin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
D010835:Phytohemagglutinins; D011498:Protein Precursors; C040782:prothymosin alpha; D013947:Thymosin