Characterization of a lipopolysaccharide encoded by a recombinant Shigella sonnei plasmid in Escherichia coli K-12.
The genetic information to synthesize the S-specific region of Shigella sonnei phase I lipopolysaccharide (LPS) is localized on a 180 kb plasmid which is lost quite readily. A recombinant plasmid derivative remaining stable in the bacteria was shown to determine the S-specific region of the LPS which is completely identical with that of a S. sonnei phase I strain following transfer in Escherichia coli K-12. However, the length control in polysaccharide biosynthesis is lost at least partially.
Seltmann
G
G
Robert-Koch-Institut des Bundesgesundheitsamts, Wernigerode, Germany.
Knirel
Y A
YA
Shashkov
A S
AS
Tschäpe
H
H
D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens; D010957:Plasmids; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D011995:Recombination, Genetic; D012764:Shigella sonnei / Q000235:genetics* / Q000378:metabolism
D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial