[Study on the recovery of 4 types of cultured cell after contacting varieties of dental casting alloys].
Four types of cultured cell (Gin-1, Chang Liver, HEP-2 and L-929) were used in vitro to determine the cytotoxicity of 12 Chinese-Japanese Dental Casting Alloys from cell recovery ability. A new cytocompatibility detecting method--cell recovery test was established and recommended. This method can be able to observe the state of cell recovery through self growth cycle, diminish the side-effect of dead cell and its products on the living cells, nearly mimic the environment in vivo and study the inherited toxicology of cells. The application of computer photo-pattern analysis technique can acquire the objective and accurate data directly.
Sun
J
J
Biomedical Materials research laboratory, Shanghai Second Medical University.
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D003722:Dental Alloys / Q000633:toxicity*; D005347:Fibroblasts / Q000166:cytology; D005881:Gingiva / Q000166:cytology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007822:Laryngeal Neoplasms / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology; D008422:Materials Testing; D051379:Mice; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003722:Dental Alloys