Effects of methyl palmoxirate on hypoxic rat atria.
Isolated rat atria in hypoxia released lactate into the bathing medium and underwent a decline of the contraction frequency which, in some cases led to a complete cessation of the pacemaker activity. A pronounced fall in the peak developed tension and a rise in the resting tension also appeared. The atria from 24 h fasted rats, which oxidize faster their reserve lipids than those from fed rats, exhibited greater functional disturbances during hypoxia, a lower lactate output and a smaller recovery of peak tension upon reoxygenation. Methyl palmoxirate, which is a selective inhibitor of carnitine palmitoyltransferase I, attenuated the decline of the beating rate and the rise of the resting tension in both groups of rats and the incidence of atrial arrest in the fasted rat group. The fall in the peak tension, lactate output and recovery upon reoxygenation were not altered by the inhibitor. These data indicate that methyl palmoxirate alleviates some of the hypoxic functional derangements. Hence, it may be inferred that inhibiting the oxidation of the fatty acid derived from the endogenous triacylglycerol is beneficial during oxygen-limited conditions and that these effects could not be ascribed to changes in the glycolytic flux.
Varela
A
A
Cátedra de Fisiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Carregal
M
M
Bruno-Magnasco
C
C
Espósito
S
S
Savino
E A
EA
D000693:Anaerobiosis; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000037:antagonists & inhibitors*; D015687:Cell Hypoxia; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D004852:Epoxy Compounds / Q000494:pharmacology*; D005215:Fasting; D005260:Female; D006325:Heart Atria / Q000187:drug effects* / Q000378:metabolism; D006329:Heart Conduction System / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008066:Lipolysis / Q000187:drug effects*; D009200:Myocardial Contraction / Q000187:drug effects; D009924:Organ Culture Techniques; D011422:Propionates / Q000494:pharmacology*; D051381:Rats; D014280:Triglycerides / Q000378:metabolism
D004852:Epoxy Compounds; D007773:Lactates; D011422:Propionates; D014280:Triglycerides; D019344:Lactic Acid; D002334:Carnitine O-Palmitoyltransferase; C017522:methyl 2-tetradecylglycidate; D004121:Dimethyl Sulfoxide