title: Segregation of altered parental properties in fusions between Saccharomyces cerevisiae and the D-xylose fermenting yeasts Candida shehatae and Pichia stipitis.
abstract: A prototrophic strain of Saccharomyces cerevisiae CSIR Y190 MATa xyl-, resistant to high levels of ethanol, was hybridized with xylose-fermenting, auxotrophic mutants of Candida shehatae and Pichia stipitis through polyethylene glycol-induced protoplast fusion in an attempt to produce ethanol-tolerant, xylose-fermenting hybrids. Mononucleate fusants were obtained, but these dissociated into a mixture of parental-type segregants. Purified Candida- and Pichia-resembling segregants failed to acquire improved ethanol tolerance but expressed other novel properties of S. cerevisiae, suggesting that karyogamy was impaired after internuclear gene transfer.
authors.affiliation: Department of Biochemistry, University of Durban-Westville, South Africa.
authors.forename: A S
authors.initials: AS
authors.lastname: Gupthar
mesh_terms: D002175:Candida / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002459:Cell Fusion; D004352:Drug Resistance, Microbial; D000431:Ethanol / Q000494:pharmacology; D005818:Genetic Engineering; D007218:Industrial Microbiology; D010843:Pichia / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D011523:Protoplasts; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D014994:Xylose / Q000378:metabolism
chemical_list: D000431:Ethanol; D014994:Xylose