[Properties of skeletal muscle sarcoplasmic reticulum membranes prepared in the presence of dithiothreitol].
The authors studied the effect of dithiothreitol (DTT), an agent modulating the redox state of SH-groups, on functional properties of sarcoplasmic reticulum membranes of skeletal muscle. DTT (5 mmol.l-1) exerted a protective effect not only on (Ca2 + Mg2)-ATPase activity but also on the activity of Ca2+ uptake by vesicles, while the most pronounced effect was recorded at low levels of ionized Ca2+. The presence of DTT in the isolation medium decreased also passive permeability of membranes for Ca2+. In the light of the results obtained, modulation of the redox state is recommended for each isolation of membranes designed for the study of ion transport.
Bezáková
G
G
Ustav lekárskej biochémie Jeseniovej LFUK, Martine, CSFR.
Lehotský
J
J
D000818:Animals; D002118:Calcium / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology*; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism
D002118:Calcium; D004229:Dithiothreitol
Vlastnosti membrán sarkoplazmatického retikula kostrového svalu pripravených za prítomnosti ditiotreitolu.