Juxtaglomerular cells cultured on a reconstituted basement membrane.
Circulating renin levels are regulated by release from juxtaglomerular (JG) cells. Here, for the first time, we describe the primary culture of rat juxtaglomerular cells on a reconstituted basement membrane. In addition, primary cultures were transformed with a temperature-sensitive SV40 large T antigen gene to promote the development of a continuous JG cell line. Both primary cultures and transformed JG cells maintain a highly differentiated state and secrete active renin. These preparations now provide a system in which characterization of the cellular mechanisms of regulation of renin synthesis and release is possible.
Rayson
B M
BM
Department of Physiology, Cornell University Medical College-New York Hospital, New York 10021.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001485:Basement Membrane; D002455:Cell Division; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D015759:Ionomycin / Q000494:pharmacology; D007606:Juxtaglomerular Apparatus / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012083:Renin / Q000378:metabolism; D015290:Second Messenger Systems; D013539:Simian virus 40 / Q000235:genetics; D013873:Thionucleotides / Q000494:pharmacology
D000952:Antigens, Polyomavirus Transforming; D012333:RNA, Messenger; D013873:Thionucleotides; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D015759:Ionomycin; D000242:Cyclic AMP; D012083:Renin