[Cytofluorimetric research on the content of glycogen and its fractions in the hepatocytes of patients with liver cirrhosis of different etiologies].
By cytofluorometric method, a study was made of the total glycogen and its two fractions in liver parenchymal cells both in the donors (20 men) and in patients with cirrhosis of different etiology (39 men). The examination was performed on preparations--smears of isolated hepatocytes, obtained from the live functional liver biopsies. The quantitative analysis has shown an increase in the total glycogen content in hepatocytes of patients with cirrhosis by 3 times compared to the norm, and this increase is independent on the etiology of liver cirrhosis. To study the mechanism of the discovered glycogenosis, the activity of key enzymes of glycogenolyses was determined. It was shown that glucose-6-phosphatase and glycogen-phosphorylase activity in the liver with cirrhosis was lower than in the norm. The most considerable changes were shown in hepatocytes of patients with liver cirrhosis in fractional glycogen composition and, even more significant, in the content of a hard soluble fraction. The hard soluble fraction portion was higher in hepatocytes of the patients with liver cirrhosis of alcohol etiology. The quantitative analysis of glycogen fraction contents in liver cells may be the best marker in the differential diagnosis of symptomless elapsing liver cirrhosis.
Kudriavtseva
M V
MV
Emel'ianov
A V
AV
Sakuta
G A
GA
Skorina
A D
AD
Sleptsova
L A
LA
Kudriavtsev
B N
BN
D000328:Adult; D002908:Chronic Disease; D005434:Flow Cytometry; D006509:Hepatitis B / Q000150:complications; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000209:etiology / Q000378:metabolism*; D008104:Liver Cirrhosis, Alcoholic / Q000209:etiology / Q000378:metabolism*; D008112:Liver Glycogen / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D008112:Liver Glycogen
Tsitofluorimetricheskoe issledovanie soderzhaniia glikogena i ego fraktsiĭ v gepatotsitakh bol'nykh tsirrozom pecheni razlichnoĭ étiologii.