Selective detection of Mycoplasma fermentans by polymerase chain reaction and by using a nucleotide sequence within the insertion sequence-like element.
A new assay using the polymerase chain reaction to amplify a 206-nucleotide specific gene sequence within the insertion sequence-like element of Mycoplasma fermentans has been developed. The unique insertion sequence-like element exists in multiple copies in the M. fermentans genome. The assay selectively amplifies DNA from all strains of M. fermentans tested. In contrast, DNA from other species of human and nonhuman mycoplasmas, common tissue culture-contaminating mycoplasmas, and bacteria, as well as human, monkey, and mouse tissues do not produce the amplified DNA products specific for M. fermentans.
Wang
R Y
RY
Department of Infectious and Parasitic Diseases Pathology, Armed Forces Institute of Pathology, Washington, D.C. 20306-6000.
Hu
W S
WS
Dawson
M S
MS
Shih
J W
JW
Lo
S C
SC
D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016858:Mycoplasma fermentans / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
D004251:DNA Transposable Elements; D004269:DNA, Bacterial