An indirect fluorescent antibody (IFA) test for the serodiagnosis of Kala-Azar.
The IFA test developed using Leishmania donovani promastigote and amastigote antigens showed very high antibody titre in clinically and parasitologically established cases (30) of kala-azar, the geometrical mean reciprocal titre (GMRT) being 870 +/- 5.4 and 5370 +/- 1.80 respectively with the two antigens. In contrast, the GMRT of normal subjects of endemic area was only 12.44 +/- 6.19 and 80.35 +/- 1.66 respectively with these antigens. None of the subjects from non-endemic area suffering with other parasitic diseases, such as malaria, filaria, amoebiasis, leprosy or tuberculosis (20 cases each) gave a positive response to any of the antigen. The test holds promise in the diagnosis of Kala-azar.
Choudhry
A
A
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Puri
A
A
Guru
P Y
PY
Saxena
R P
RP
Saxena
K C
KC
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007893:Leishmania donovani / Q000302:isolation & purification; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000913:Antibodies, Protozoan