Isoelectric focusing patterns of beta-lactamases in the rapidly growing mycobacteria.
beta-lactamases from 259 strains of rapidly growing mycobacteria that included the third biovariant complex of Mycobacterium fortuitum, M. peregrinum, M. abscessus, M. chelonae, the M. chelonae-like organisms (MCLO), and M. smegmatis were analyzed by isoelectric focusing (IEF). All isolates produced acidic beta-lactamases with major band isoelectric points (pIs) between 4.4 and 6.0. Each of the 6 taxonomic groups exhibited 1 or 2 characteristic beta-lactamase IEF patterns. Heterogeneity among IEF patterns was evident in 5 of the 6 groups, however, and was greatest among the third biovariant complex of M. fortuitum. beta-lactamase patterns correlated with previously identified taxonomic subgroups of M. smegmatis and the third biovariant complex of M. fortuitum. beta-lactamase IEF analysis of MCLO strains isolated from two outbreaks demonstrated its possible usefulness for epidemiologic evaluation.
Zhang
Y
Y
Department of Microbiology, University of Texas Health Center, Tyler 75710.
Wallace
R J
RJ
Steingrube
V A
VA
Brown
B A
BA
Nash
R
R
Silcox
A
A
Tsukamura
M
M
D000818:Animals; D002415:Cats; D006801:Humans; D007525:Isoelectric Focusing; D009161:Mycobacterium / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D016926:Mycobacterium chelonae / Q000201:enzymology; D009170:Nontuberculous Mycobacteria / Q000201:enzymology; D001618:beta-Lactamases / Q000032:analysis*
D001618:beta-Lactamases