Inhibition of poliovirus RNA synthesis by brefeldin A.
Brefeldin A (BFA), a fungal metabolite that blocks transport of newly synthesized proteins from the endoplasmic reticulum, was found to inhibit poliovirus replication 10(5)- to 10(6)-fold. BFA does not inhibit entry of poliovirus into the cell or translation of viral RNA. Poliovirus RNA synthesis, however, is completely inhibited by BFA. A specific class of membranous vesicles, with which the poliovirus replication complex is physically associated, is known to proliferate in poliovirus-infected cells. BFA may inhibit poliovirus replication by preventing the formation of these vesicles.
Maynell
L A
LA
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347.
Kirkegaard
K
K
Klymkowsky
M W
MW
D000256:Adenoviridae / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D000998:Antiviral Agents / Q000494:pharmacology*; D020126:Brefeldin A; D003517:Cyclopentanes / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis; D017955:Poliovirus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis*; D014779:Virus Replication / Q000187:drug effects* / Q000235:genetics
D000998:Antiviral Agents; D003517:Cyclopentanes; D008055:Lipids; D012367:RNA, Viral; D020126:Brefeldin A