Glial cells in coculture can increase the acetylcholinesterase activity in human brain endothelial cells.
The elements of the cholinergic system (acetylcholinesterase and choline acetyltransferase) and butyrylcholinesterase were studied in human cortical capillary samples, brain-derived endothelial cell cultures and glial cell cultures. It was shown that the elements of the cholinergic system are present in the microvessels, but the choline acetyltransferase activity may be due to contamination with cholinergic nerve terminals since no choline acetyltransferase could be demonstrated in endothelial cell cultures. The present results revealed that the activity of acetylcholinesterase is reduced in the cortical endothelial cell cultures after longer culture times, while butyrylcholinesterase activity is not altered. In a system where endothelial cells were cocultured with embryonic human brain astroglial cells for 12 days in vitro, the acetylcholinesterase activity was increased 2-fold. These results support a glial influence on the enzyme activity of the cerebral endothelium.
Pákáski
M
M
Central Research Laboratory, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Kása
P
P
D000110:Acetylcholinesterase / Q000378:metabolism*; D001253:Astrocytes / Q000502:physiology*; D002091:Butyrylcholinesterase / Q000378:metabolism; D002450:Cell Communication; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000098:blood supply / Q000502:physiology*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004622:Embryo, Mammalian; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008833:Microcirculation; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*
D005904:Glial Fibrillary Acidic Protein; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase