title: Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library.
abstract: A new approach for the isolation of chromosome-specific subsets from a human genomic yeast artificial chromosome (YAC) library is described. It is based on the hybridization with an Alu polymerase chain reaction (PCR) probe. We screened a 1.5 genome equivalent YAC library of megabase insert size with Alu PCR products amplified from hybrid cell lines containing human chromosome 21, and identified a subset of 63 clones representative of this chromosome. The majority of clones were assigned to chromosome 21 by the presence of specific STSs and in situ hybridization. Twenty-nine of 36 STSs that we tested were detected in the subset, and a contig spanning 20 centimorgans in the genetic map and containing 8 STSs in 4 YACs was identified. The proposed approach can greatly speed efforts to construct physical maps of the human genome.
authors.affiliation: Centre d'Etude du Polymorphisme Humain, Paris, France.
authors.forename: I M
authors.initials: IM
authors.lastname: Chumakov
authors.forename: I
authors.initials: I
authors.lastname: Le Gall
authors.forename: A
authors.initials: A
authors.lastname: Billault
authors.forename: P
authors.initials: P
authors.lastname: Ougen
authors.forename: P
authors.initials: P
authors.lastname: Soularue
authors.forename: S
authors.initials: S
authors.lastname: Guillou
authors.forename: P
authors.initials: P
authors.lastname: Rigault
authors.forename: H
authors.initials: H
authors.lastname: Bui
authors.forename: M F
authors.initials: MF
authors.lastname: De Tand
authors.forename: E
authors.initials: E
authors.lastname: Barillot
mesh_terms: D001483:Base Sequence; D015825:Chromosomes, Fungal; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015723:Gene Library; D005821:Genetic Techniques; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA