[Protein phosphorylations in granulocyte/macrophage colony-stimulating factor-stimulated human peripheral blood eosinophils].
Peripheral blood eosinophils from normal healthy subjects were isolated on a Percoll gradient and were incubated with [32P] orthophosphoric acid. 32P-labeled eosinophils were washed and stimulated with GM-CSF under different conditions. After reaction was stopped, SDS/PAG electrophoresis was performed along with autoradiographs to determine the incorporation of 32P into proteins. GM-CSF-stimulated eosinophils produced an increase of 32P incorporation into the bands in the 31 kDa and 35 kDa areas after 30 sec and 1 min of stimulation. The increase of 32P incorporation was dependent on Mg2+ concentration and temperature, suggesting that proteins in the eosinophils with apparent molecular weights of 31 kDa and 35 kDa can be phosphorylated with the stimulation of GM-CSF.
Shimizu
Y
Y
First Department of Internal Medicine, Gunma University School of Medicine.
Kurosawa
M
M
D000328:Adult; D001798:Blood Proteins / Q000378:metabolism*; D004804:Eosinophils / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D008297:Male; D010758:Phosphorus / Q000378:metabolism*; D013268:Stimulation, Chemical
D001798:Blood Proteins; D010758:Phosphorus; D016178:Granulocyte-Macrophage Colony-Stimulating Factor