A search for immunocontraceptive agent from marine sources--role of antispermatheca globulin of Telescopium telescopium on fertility regulation in male rat.
Polyclonal antisera were developed in rabbits against 'spermatheca', the reproductive gland of T. telescopium, a marine mollusc. The gland contains spermatozoa. Antisera and its IgG fraction (ASTG) indicated common antigenic determinants by immunodiffusion and had titer values 81920 and 1280 against crude antigen extract. Cycling female rats when exposed to passively immunized male rats with different doses of ASTG, had reduction in implantation sites and litter size. Females had pseudopregnancy when exposed to higher doses of immunized males and had normal cycle after 20 days in average. ASTG in male rats caused decrease in weight of the reproductive glands, alteration in sperm concentration, motility and morphology, formation of multinucleated giant cells and vacuoles leading to arrest of spermatogenesis and reduction in seminiferous tubular diameter. The effects were dose dependent with reversible infertility. The results indicate presence of a common antigenic determinants which cross-react with vertebrates and existence of common relation through phylogenetic evolution and their immune responses.
Pakrashi
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A
Reproductive Biology Research Division, Indian Institute of Chemical Biology, Calcutta.
Datta
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U
Choudhury
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D000818:Animals; D003269:Contraception, Immunologic; D003272:Contraceptive Agents, Male / Q000302:isolation & purification* / Q000494:pharmacology; D010064:Embryo Implantation; D000939:Epitopes / Q000032:analysis; D005260:Female; D005298:Fertility / Q000276:immunology*; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000494:pharmacology*; D008098:Litter Size; D008297:Male; D011247:Pregnancy; D011555:Pseudopregnancy; D011817:Rabbits / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012908:Snails; D013094:Spermatozoa / Q000276:immunology*
D003272:Contraceptive Agents, Male; D000939:Epitopes; D007074:Immunoglobulin G