Characterization of lipoprotein lipase activators from equine plasma.
Equine plasma lipoproteins were fractionated into VLDL, LDL-1, LDL-2 and HDL by density gradient ultracentrifugation. From each lipoprotein fraction, five apo C like peptides of approx. M(r) 1400, 10000, 9500, 9000 and 8000 were detected by SDS-polyacrylamide gel electrophoresis. After partial purification by Sephadex G-75, one fraction, showing a strong activation of lipoprotein lipase, was further purified by Mono Q anion exchange column. Two of the apo C like peptides (M(r) 10000 and 8000) activated the bovine milk lipoprotein lipase in vitro; only one (M(r) 9500) inhibited the lipolytic activity. This work confirms that many mammals present two apo C-II components with different molecular weights.
Le Goff
D
D
Laboratoire de Biochimie, URA 609 CNRS, Université de Caen, France.
Hannan
J
J
Maboundou
J C
JC
Ayrault-Jarrier
M
M
D000818:Animals; D001056:Apolipoproteins C / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D006736:Horses / Q000097:blood*; D066298:In Vitro Techniques; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000097:blood / Q000737:chemistry; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry; D008079:Lipoproteins, VLDL / Q000097:blood / Q000737:chemistry; D008892:Milk / Q000201:enzymology; D008970:Molecular Weight
D001056:Apolipoproteins C; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D008071:Lipoprotein Lipase