Significance of 3HOH generation from D-[2-3H]glucose.
The significance of 3HOH generation by cells exposed to D-[2-3H]glucose is reevaluated. It is proposed that such a metabolic variable, rather than being taken as an index of D-glucose phosphorylation, may provide information on the extent of D-glucose 6-phosphate and D-fructose 6-phosphate interconversion in the reaction catalyzed by phosphoglucoisomerase. In order to reach such an information, the total production of 3HOH from D-[2-3H]glucose needs to be corrected for that attributable to either the catabolism of D-[1-3H]fructose 1,6-bisphosphate or the circulation of D-[2-3H]glucose 6-phosphate in the pentose phosphate pathway. A method is introduced which allows for such a correction.
Manuel y Keenoy
B
B
Laboratory of Experimental Medicine, Brussels Free University, Belgium.
Malaisse
W J
WJ
D000818:Animals; D005635:Fructosediphosphates / Q000378:metabolism; D005636:Fructosephosphates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D006019:Glycolysis; D008954:Models, Biological; D011865:Radioisotope Dilution Technique; D014316:Tritium; D014867:Water
D005635:Fructosediphosphates; D005636:Fructosephosphates; D005958:Glucosephosphates; D014867:Water; D014316:Tritium; D019298:Glucose-6-Phosphate; C027618:fructose-6-phosphate; D005947:Glucose; C029063:fructose-1,6-diphosphate