Rapid immunostaining of live nerve for identification of sensory and motor fasciculi.
Six New Zealand white rabbits were anesthetized with pentobarbital, and sciatic nerves were exposed and cut at the mid thigh. Both proximal and distal ends were incubated directly with Blue-SAb in a micropipe of 5 mm diameter covering the nerve trunk ends for 30 minutes at room temperature. After removal of the micropipe, the nerve ends were washed with physiological saline. Blue-stained fasciculi, i.e., sensory fasciculi were seen among unstained ones under the operating microscope. This method requires no histological sections. Neural cells of the spinal cord and ganglion were cultured in RPM11640 medium containing Bright Blue. The growth and metabolism of the neural cells were tested by MTT assay and their morphology was observed. Statistical difference between the experiment and control groups was determined, indicating that Bright Blue had no effect on the neural cells and their repairing power. This rapid immunostaining technique offers a good approach for the identification and accurate coaptation of sensory fasciculi in peripheral nerve repair.
Gu
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XS
Department of Anatomy, Nantong Medical College.
Yan
Z Q
ZQ
Yan
W X
WX
Chen
C F
CF
D000818:Animals; D002478:Cells, Cultured; D009046:Motor Neurons / Q000166:cytology; D009475:Neurons, Afferent / Q000166:cytology; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000033:anatomy & histology*; D011984:Sensory Receptor Cells / Q000033:anatomy & histology*; D013116:Spinal Cord / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods; D013778:Tetrazolium Salts; D013844:Thiazoles
D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue