[Immortalization of cultured cells by the Ha-rasEJ oncogene and expression of a gene of biogenetic value].
The double recombination into NIH-3T3 cells of the cloned Ha-rasEJ oncogene and the cDNA gene of the human growth hormone (hGH) under the control of a heat inducible promoter (hsp70) allowed hGH production either in vitro using the mass culture of engineered cells in biogenerators, or in vivo after xeno-transplantation of the cells into an animal host. Therefore the in vivo synthesized hGH induced the production of anti-hGH polyclonal antibodies.
Montandon
F
F
Laboratoire de Biochimie Médicale, UFR Médecine, Dijon.
Cao
H
H
Dreano
M
M
Padieu
P
P
D016475:3T3 Cells / Q000637:transplantation*; D000818:Animals; D015870:Gene Expression; D011905:Genes, ras / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D011401:Promoter Regions, Genetic
D006360:Heat-Shock Proteins; D013006:Growth Hormone
Immortalisation de cellules en culture par l'oncogène Ha-rasEJ et expression d'un gène d'intérêt biogénétique.