Protein kinase C activity in neonatal cultured rat cardiomyocytes supplemented with docosahexaenoic acid.
In vitro studies have indicated that the 1-stearoyl, 2-arachidonyl diacylglycerol (DAG) is the most effective one for the activation of protein kinase C, although many other DAGs having a different fatty acid composition are active, but to a different extent. Using cultures of neonatal rat ventricular cells, grown in a medium enriched in docosahexaenoic acid (DHA), we previously obtained a cell population that, after alpha 1-adrenoceptor stimulation, produced a DHA enriched DAG. In this study, we have tested the "in vivo" ability of this modified DAG as protein kinase C activator, demonstrating a lower but more persistent translocation of the enzyme from cytosol to particulate fraction in the DHA treated cells. The differences in the PKC activation pattern could be explained by a different metabolism of the DHA enriched DAG by DAG kinase.
Hrelia
S
S
Dipartimento di Biochimica G. Moruzzi, University of Bologna, Italy.
Biagi
P L
PL
Turchetto
E
E
Rossi
C A
CA
Bordoni
A
A
D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport; D002478:Cells, Cultured; D003470:Culture Media; D004075:Diglycerides / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004789:Enzyme Activation; D006352:Heart Ventricles / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism; D013268:Stimulation, Chemical; D013347:Subcellular Fractions / Q000378:metabolism
D003470:Culture Media; D004075:Diglycerides; D011942:Receptors, Adrenergic, alpha; D004281:Docosahexaenoic Acids; D011493:Protein Kinase C