title: Immunofluorescence detection of the vimentin epitope in chromatin structures of cell nuclei and chromosomes.
abstract: We describe immunofluorescence detection of the vimentin epitope, recognized by monoclonal antibody VI-01, in chromatin structures of eukaryotic cell nuclei and chromosomes. The approach used is based on increased sensitivity of 5-bromodeoxyuridine (BrdU)-substituted DNA to UV irradiation-induced crosslinking of DNA with proteins in vivo, by which the proteins interacting with chromosomal DNA can be immunovisualized in situ.
authors.affiliation: Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
authors.forename: H
authors.initials: H
authors.lastname: Fidlerová
authors.forename: V
authors.initials: V
authors.lastname: Sovová
authors.forename: I
authors.initials: I
authors.lastname: Krekule
authors.forename: V
authors.initials: V
authors.lastname: Viklický
authors.forename: G
authors.initials: G
authors.lastname: Levan
mesh_terms: D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry*; D002875:Chromosomes / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000032:analysis*; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D051379:Mice; D051381:Rats; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis*
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D000939:Epitopes; D014746:Vimentin