Monoclonal antibodies recognising pol and env proteins of mammalian retroviruses.
Mouse monoclonal antibodies were generated for three 20- to 23-mer synthetic peptides whose amino acid sequences were highly conserved in the pol or env regions of 14 mammalian and two avian retroviruses. By Western-blot analysis, these antibodies recognised the epitopes in major and minor pol or env proteins of six mammalian type-C and -D retroviruses. One of the anti-env monoclonal antibodies was found to recognise all the env transmembrane proteins of six mammalian retroviruses examined.
Nishimura
N
N
Department of Microbiology, Keio University School of Medicine, Tokyo, Japan.
Kato
S
S
Ishikawa
H
H
Takano
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000918:Antibody Specificity; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology*; D015684:Gene Products, pol / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D012190:Retroviridae / Q000235:genetics / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015686:Gene Products, env; D015684:Gene Products, pol; D010446:Peptide Fragments