title: Origin of sequence specific cleavage of DNA by bleomycins.
abstract: A new DNA photocleaving agent which contains bleomycin A2's DNA binding portion and its mono- and terthiazole analogues have been designed and synthesized, and their DNA binding mode and cleavage base specificity have been studied. The photoactive p-nitrobenzoyl group attached at the end of molecules cleaves DNA on UV irradiation. All the oligo-thiazole compounds exhibited high sequence specificity in DNA scission. The bithiazole derivative did not cleave DNA at or near 5'-GpT-3' or 5'-GpC-3' as expected from widely believed DNA binding mechanism of the antibiotics.
authors.affiliation: Department of Chemistry, College of Arts and Sciences, University of Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Kuroda
authors.forename: M
authors.initials: M
authors.lastname: Shinomiya
authors.forename: M
authors.initials: M
authors.lastname: Otsuka
mesh_terms: D001761:Bleomycin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D010777:Photochemistry; D014466:Ultraviolet Rays
chemical_list: D001761:Bleomycin; D004247:DNA