title: The effect of Lasso herbicide on human immune function as measured by in vitro assays.
abstract: Using in vitro assays, this study was undertaken to determine whether the components of Lasso herbicide formulation had an effect on the human immune system. Mononuclear cells from human peripheral blood were exposed to analytical alachlor, alachlor conjugated to human serum albumin or Lasso formulation over a concentration range from .01 microM-1.0 microM. The effects of the test materials on the following immunological functions were determined: lymphocyte proliferation induced by mitogen or antigen; antibody synthesis of IgG and IgM isotypes in pokeweed stimulated mononuclear cell cultures; cytotoxic T cell proliferation; lysis of target cells by natural killer cells and lymphokine activated killer cells. The data demonstrated that the test compounds had no significant, dose related effect on the function of immunocompetent cells. Hence, the data suggest that the components of the Lasso formulation have no effect on the human immune system.
authors.affiliation: Environmental Health Laboratory Monsanto Agricultural Company, Unit of Monsanto Company, St. Louis, MO 63110.
authors.forename: D K
authors.initials: DK
authors.lastname: Flaherty
authors.forename: P A
authors.initials: PA
authors.lastname: Winzenburger
authors.forename: C J
authors.initials: CJ
authors.lastname: Gross
authors.forename: K L
authors.initials: KL
authors.lastname: McGarity
authors.forename: M
authors.initials: M
authors.lastname: Panyik
authors.forename: P
authors.initials: P
authors.lastname: Feng
mesh_terms: D000081:Acetamides / Q000276:immunology / Q000633:toxicity*; D000328:Adult; D000917:Antibody Formation / Q000187:drug effects*; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology; D005260:Female; D006540:Herbicides / Q000276:immunology / Q000633:toxicity*; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000633:toxicity*; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D012709:Serum Albumin / Q000494:pharmacology
chemical_list: D000081:Acetamides; D006540:Herbicides; D007166:Immunosuppressive Agents; D008934:Mitogens; D012709:Serum Albumin; C000188:alachlor; D016572:Cyclosporine