title: Dynamical conformational change of DNA induced by synthetic polymers: direct observation by fluorescence microscopy.
abstract: Brownian motion and Higher-order structure of DNA molecules complexed with synthetic polymers: poly-L-arginine (pArg) and polyacrylamide (pAAm), were observed using fluorescence microscopy. Quantitative analyses of the conformation and motion of the complexes demonstrate that these two polymers influence the structure of DNA molecules in an opposite way. The electrostatic binding of pArg induces contraction of DNA, whereas the intercalative pseudo-grafting of pAAm extends DNA.
authors.affiliation: Graduate School of Human Informatics, Nagoya University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Minagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuzawa
authors.forename: K
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: C
authors.initials: C
authors.lastname: Nishimura
authors.forename: M
authors.initials: M
authors.lastname: Maeda
authors.forename: M
authors.initials: M
authors.lastname: Takagi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D004247:DNA; D010455:Peptides; D000099166:Polyacrylamides; C015462:polyarginine