title: An indirect fluorescent antibody (IFA) test for the serodiagnosis of Kala-Azar.
abstract: The IFA test developed using Leishmania donovani promastigote and amastigote antigens showed very high antibody titre in clinically and parasitologically established cases (30) of kala-azar, the geometrical mean reciprocal titre (GMRT) being 870 +/- 5.4 and 5370 +/- 1.80 respectively with the two antigens. In contrast, the GMRT of normal subjects of endemic area was only 12.44 +/- 6.19 and 80.35 +/- 1.66 respectively with these antigens. None of the subjects from non-endemic area suffering with other parasitic diseases, such as malaria, filaria, amoebiasis, leprosy or tuberculosis (20 cases each) gave a positive response to any of the antigen. The test holds promise in the diagnosis of Kala-azar.
authors.affiliation: Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
authors.forename: A
authors.initials: A
authors.lastname: Choudhry
authors.forename: A
authors.initials: A
authors.lastname: Puri
authors.forename: P Y
authors.initials: PY
authors.lastname: Guru
authors.forename: R P
authors.initials: RP
authors.lastname: Saxena
authors.forename: K C
authors.initials: KC
authors.lastname: Saxena
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007893:Leishmania donovani / Q000302:isolation & purification; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan