title: Matrix-assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared.
abstract: A number of different matrices have been tested and compared for ultraviolet and infrared (UV and IR) matrix-assisted laser desorption/ionization (MALDI) of oligodeoxyribonucleotides and mixtures thereof, as well as ribonucleic acids (tRNA from yeast and rRNA from E. coli). A new technique for removing alkali cations from nucleic acid samples during sample preparation on the sample support is demonstrated. The amount of oligonucleotide sample consumed during a typical measurement in IR-MALDI-MS was determined.
authors.affiliation: Institute for Medical Physics, University of Münster, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Nordhoff
authors.forename: A
authors.initials: A
authors.lastname: Ingendoh
authors.forename: R
authors.initials: R
authors.lastname: Cramer
authors.forename: A
authors.initials: A
authors.lastname: Overberg
authors.forename: B
authors.initials: B
authors.lastname: Stahl
authors.forename: M
authors.initials: M
authors.lastname: Karas
authors.forename: F
authors.initials: F
authors.lastname: Hillenkamp
authors.forename: P F
authors.initials: PF
authors.lastname: Crain
mesh_terms: D000804:Angiotensin II / Q000032:analysis; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000737:chemistry; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D012313:RNA / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009838:Oligodeoxyribonucleotides; D012335:RNA, Ribosomal; D000804:Angiotensin II; D012313:RNA; D012343:RNA, Transfer