Construction of a genetically engineered strain producing propionylspiramycin.
Our work has demonstrated the cloning of propionyl acylase gene and the expression of S. mycarofaciens mutant in S. lividans TK54. In this paper, we report the transformation of pIJM9 recombinant plasmid containing the propionyl acylase gene into spiramycin producer S. spiramyceticus. The results of colony hybridization and Southern hybridization showed that No. 61 transformant harbored the pIJM9 recombinant plasmid. TLC and bioautography showed that the Rf value of one component of the fermentation products of No. 61 transformant was similar to that of propionylspiramycin. The HPLC retention time of the components of the fermentation products of No. 61 transformant and that of propionylspiramycin were also similar. Mass spectrum analysis showed that there was propionylspiramycin II in the fermentation products of No. 61 transformant. According to these results, No. 61 transformant is shown to be a genetic engineered strain producing propionylspiramycin.
Li
Y
Y
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing.
Liu
B
B
Li
X
X
Yang
Y
Y
Wang
S
S
D000217:Acyltransferases / Q000235:genetics; D001709:Biotechnology; D003001:Cloning, Molecular; D015870:Gene Expression; D005818:Genetic Engineering; D015572:Spiramycin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic
C080505:4''-O-propionylspiramycin; D015572:Spiramycin; D000217:Acyltransferases; C023906:spiramycin I 3-hydroxyl acylase