Culture of human osteoblasts on demineralised human bone. Possible means of graft enhancement.
We cultured human osteoblasts from trabecular bone explants and confirmed their phenotype by alkaline phosphatase assay, increased cyclic adenosine monophosphate production in response to prostaglandin E2 and radiographic micro-analysis of nodules of calcification. The osteoblasts were seeded on to demineralised human bone fragments and examined at ten-day intervals over a 50-day period by scanning electron microscopy. During this time the bank bone became progressively repopulated by the cultured osteoblasts. This system may offer a means of graft enhancement in elective orthopaedic and maxillofacial surgery by delivery of cultured autologous human osteoblasts to bone defects.
Nolan
P C
PC
School of Biomedical Science, Queen's University of Belfast, Northern Ireland.
Nicholas
R M
RM
Mulholland
B J
BJ
Mollan
R A
RA
Wilson
D J
DJ
D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology*; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D003648:Decalcification Technique; D015232:Dinoprostone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006082:Graft Enhancement, Immunologic / Q000379:methods*; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010641:Phenotype
D000242:Cyclic AMP; D000469:Alkaline Phosphatase; D015232:Dinoprostone