A directional, high-frequency chromosomal mobilization system for genetic mapping of Rhizobium meliloti.
A system for mapping of the Rhizobium meliloti chromosome that utilizes transposon Tn5-Mob, which carries the mobilization site of IncP plasmid RP4 (R. Simon, Mol. Gen. Genet. 196:413-420, 1984), was developed. Insertions of Tn5-Mob that were located at particular sites on the R. meliloti chromosome were isolated and served as origins of high-frequency chromosomal transfer when IncP tra functions were provided in trans. This approach is, in principle, applicable to any gram-negative bacterium in which Tn5 can transpose and into which IncP plasmids can conjugate.
Klein
S
S
Biology Department, Massachusetts Institute of Technology, Cambridge 02139.
Lohman
K
K
Clover
R
R
Walker
G C
GC
Signer
E R
ER
D002874:Chromosome Mapping / Q000379:methods*; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016962:Sinorhizobium meliloti / Q000235:genetics*
D004251:DNA Transposable Elements