Voltage-dependent calcium entry in confluent bovine capillary endothelial cells.
Confluent bovine capillary endothelial cells display, when examined for voltage-dependent calcium entries using cell-attached channel recordings, two types of Ca2+ channels (4 and 23.5 pS in 110 mM Ba2+) both sensitive to the dihydropyridine Ca agonist BAY K 8644. In contrast to isolated cells, confluent cells display no T-type, low threshold activity, and Ca currents were typically only elicited at very depolarized potentials. In these cells, voltage-dependent calcium entries will only be made operative by substances able to shift their activation towards the resting potential.
Bossu
J L
JL
Laboratoire d'Etude des Régulations Physiologiques, CNRS associé à l'Université Louis Pasteur, Strasbourg, France.
Elhamdani
A
A
Feltz
A
A
D000313:Adrenal Medulla; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism*; D002196:Capillaries / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured / Q000378:metabolism; D004553:Electric Conductivity; D004730:Endothelium, Vascular / Q000378:metabolism*; D015640:Ion Channel Gating; D008564:Membrane Potentials
D015220:Calcium Channels; D002118:Calcium