title: Structure of the sugar-phosphate moiety of lipid A from lipooligosaccharide of Neisseria meningitidis group B, strain BC5S No. 125. Hydrolytic stability of phosphate and pyrophosphate substituents.
abstract: On the basis of chemical and NMR data the partial structure of lipid A from lipooligosaccharide (LOS) of Neisseria meningitidis group B, strain BC5S No 125 was established. Lipid A consisted of disaccharide 2-deoxy-6-O-[2-deoxy-2-(3-hydroxytetradecanoylamino)- beta-gluco-pyranosyl]-2-(3-hydroxytetradecanoylamino)- alpha-glucopyranose carrying the beta-(2-aminoethyl)pyrophosphate residue at 0-4' and the pyrophosphate or phosphate residue at 0-1. On hydrolysis of the acidic form of LOS with 1% acetic acid the substituent at 0-1 was practically completely removed whereas that at 0-4' was stable. The analogous hydrolysis of the Mg-salt of LOS was accompanied by splitting off the pyrophosphate linkage in the substituent at 0-4'. Hydrolysis of LOS at pH 4.5 in the presence of SDS led mainly to a lipid A preparation retaining both pyrophosphate residues.
authors.affiliation: Institute of Immunology, Ministry of Health of the USSR, Moscow.
authors.forename: V L
authors.initials: VL
authors.lastname: L'vov
authors.forename: I K
authors.initials: IK
authors.lastname: Verner
authors.lastname: Musina LYu
authors.forename: A V
authors.initials: AV
authors.lastname: Rodionov
authors.forename: A V
authors.initials: AV
authors.lastname: Ignatenko
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D011756:Diphosphates / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D006868:Hydrolysis; D008050:Lipid A / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009345:Neisseria meningitidis / Q000737:chemistry* / Q000276:immunology; D010084:Oxidation-Reduction; D010710:Phosphates / Q000737:chemistry*
chemical_list: D000942:Antigens, Bacterial; D011756:Diphosphates; D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D010710:Phosphates; C023023:lipid-linked oligosaccharides