Specific incorporation of kinetin into eukaryotic and prokaryotic transfer ribonucleic acid molecules.
We show that kinetin, a non-natural product with strong cytokinin activity, is incorporated into prokaryotic and eukaryotic tRNAs in the exchange reaction catalysed by a putative tRNA-kinetin transglycosylase. We also show that kinetin is specifically incorporated into E. coli tRNA(Tyr) and most probably at position 37. To our knowledge, this is the first report of a nucleic acid base exchange reaction occurring at this position.
Barciszewski
J
J
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan.
Otzen
D
D
Rattan
S I
SI
Clark
B F
BF
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D002384:Catalysis; D004926:Escherichia coli / Q000737:chemistry; D007701:Kinetin; D012329:RNA, Bacterial / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism*; D012365:RNA, Transfer, Tyr / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry
D012329:RNA, Bacterial; D012331:RNA, Fungal; D012365:RNA, Transfer, Tyr; D000225:Adenine; D007701:Kinetin