[The urinary mutagenicity test in monitoring exposure to aromatic polycyclic hydrocarbons in workers in the aluminum industry].
The sensitivity of 3 urinary mutagenicity tests was assayed: the plate test, the fluctuation test and the micropreincubation test, in order to assess their possible use in monitoring human exposure to polycyclic aromatic hydrocarbons (PAH). Urine samples from workers of an anode production plant exposed to coal tar and from psoriatic patients undergoing treatment with coal-tar ointments were tested for mutagenic activity on strain TA98 Salmonella typhimurium, in the presence of the microsome fraction and deconjugating enzymes. Parallelly, the urinary concentration of PAH metabolites or one of their trace metabolites, 1-hydroxypyrene, was determined. Increased levels of PAH metabolites were observed in the urine of anode production workers after a work shift compared with controls. Results of the plate test and the fluctuation test performed on urine of exposed subjects, both smokers and nonsmokers, showed mutagenicity values similar to the controls. Much higher 1-hydroxypyrene concentrations were found in the urine of psoriatic patients treated with coal tar than in post-shift urine of anode production workers. The urine of the former was also mutagenic in the 3 mutagenicity tests used. The minimum mean dose of PAH metabolites was calculated, expressed as quantity of 1-hydroxypyrene, that would give a mutagenic response in the 3 tests: the micropreincubation test was found to be about 100 times more sensitive than the plate test and about 30 times more sensitive than the fluctuation test. The theoretical minimum urinary concentration of 1-hydroxypyrene detectable by each test was determined: the micropreincubation test was 15 times more sensitive than the plate test and 7 times more sensitive than the fluctuation test.(ABSTRACT TRUNCATED AT 250 WORDS)
Granella
M
M
Instituto di Medicina del Lavoro, Università di Padova.
Venier
P
P
Zordan
M
M
Clonfero
E
E
D000535:Aluminum; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D008669:Metallurgy; D009152:Mutagenicity Tests / Q000379:methods; D016273:Occupational Exposure; D011083:Polycyclic Compounds / Q000633:toxicity / Q000652:urine*; D011721:Pyrenes / Q000032:analysis; D012486:Salmonella typhimurium / Q000187:drug effects; D012680:Sensitivity and Specificity; D012907:Smoking / Q000652:urine
D011083:Polycyclic Compounds; D011721:Pyrenes; D000535:Aluminum; C033146:1-hydroxypyrene
Test di mutangenicità urinaria nel monitoraggio dell'esposizione ad idrocarburi policiclici aromatici di lavoratori dell'industria di alluminio.