title: Robotic solid phase extraction and high performance liquid chromatographic analysis of ranitidine in serum or plasma.
abstract: A fully automated assay for the analysis of ranitidine in serum and plasma, with and without an internal standard, was validated. It utilizes robotic solid phase extraction with on-line high performance liquid chromatographic (HPLC) analysis. The ruggedness of the assay was demonstrated over a three-year period. A Zymark Py Technology II robotic system was used for serial processing from initial aspiration of samples from original collection containers, to final direct injection onto the on-line HPLC system. Automated serial processing with on-line analysis provided uniform sample history and increased productivity by freeing the chemist to analyse data and perform other tasks. The solid phase extraction efficiency was 94% throughout the assay range of 10-250 ng/mL. The coefficients of variation for within- and between-day quality control samples ranged from 1 to 6% and 1 to 5%, respectively. Mean accuracy for between-day standards and quality control results ranged from 97 to 102% of the respective theoretical concentrations.
authors.affiliation: Glaxo Inc. Research Institute, Research Triangle Park, NC 27709.
authors.forename: T L
authors.initials: TL
authors.lastname: Lloyd
authors.forename: T B
authors.initials: TB
authors.lastname: Perschy
authors.forename: A E
authors.initials: AE
authors.lastname: Gooding
authors.forename: J J
authors.initials: JJ
authors.lastname: Tomlinson
mesh_terms: D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011342:Procainamide / Q000097:blood; D011786:Quality Control; D011899:Ranitidine / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D012371:Robotics; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011899:Ranitidine; D011342:Procainamide