title: Crystallization of proton channel peptides.
abstract: Crystals have been grown of two similar peptides that form ion-conducting channels in diphytanoyl phosphatidylcholine bilayers. These crystals were grown by slow evaporation of the organic solvent, 2,2,2-trifluoroethanol. Crystals of one of the peptides have been characterized by X-ray diffraction, and X-ray data have been measured to 2.3 A resolution. Earlier it was proposed that the ion-conducting channels formed by these peptides consist of four peptides associated as a parallel alpha-helical tetramer. On the basis of the space group and unit cell dimensions of the crystals, a packing scheme for the peptide is proposed that is consistent with a tetrameric channel.
authors.affiliation: Molecular Biology Institute, University of California, Los Angeles 90024-1570.
authors.forename: B
authors.initials: B
authors.lastname: Lovejoy
authors.forename: K S
authors.initials: KS
authors.lastname: Akerfeldt
authors.forename: W F
authors.initials: WF
authors.lastname: DeGrado
authors.forename: D
authors.initials: D
authors.lastname: Eisenberg
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D007202:Indicators and Reagents; D007473:Ion Channels; D008051:Lipid Bilayers; D046911:Macromolecular Substances; D008961:Models, Structural; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D010713:Phosphatidylcholines; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D007202:Indicators and Reagents; D007473:Ion Channels; D008051:Lipid Bilayers; D046911:Macromolecular Substances; D009842:Oligopeptides; D010713:Phosphatidylcholines; C021016:1,2-diphytanoylphosphatidylcholine