[The correlation between the acidic composition of diacylglycerol and protein kinase C activation in cultures of rat cardiomyocytes].
We have studied the fatty acid composition of the diacylglycerol produced after different stimulation times with an alpha 1-agonist (phenylephrine) in cultures of beating neonatal rat cardiomyocytes, and we have related the acidic pattern to the time course of the translocation of protein kinase C from cytosol to the membrane, both in control cells and in cells grown in a medium supplemented with docosahexaenoic acid. Gas chromatography of the diacylglycerol produced after stimulation revealed significant differences between control cells and cells grown in the docosahexaenoic acid supplemented medium. In the control cells, in the early stimulation times, the higher protein kinase C activity was due to a higher relative molar content of arachidonic acid in the diacylglycerol; in the docosahexaenoic acid treated cells the lower but more persistent activation of the membrane-bound protein kinase C might be sustained by an enrichment of diacylglycerol with docosahexaenoic acid. The modification of the fatty acid composition of diacylglycerol can cause an alteration in the response of the cells to alpha 1-adrenoceptor stimulation.
Bordoni
A
A
Dipartimento di Biochimica G Moruzzi, Università degli Studi, Bologna.
Biagi
P L
PL
Turchetto
E
E
Rossi
C A
CA
Hrelia
S
S
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002849:Chromatography, Gas; D004075:Diglycerides / Q000032:analysis / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D006321:Heart / Q000187:drug effects; D009206:Myocardium / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D010656:Phenylephrine / Q000494:pharmacology; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013268:Stimulation, Chemical; D013997:Time Factors
D004075:Diglycerides; D005227:Fatty Acids; D010656:Phenylephrine; D004281:Docosahexaenoic Acids; D011493:Protein Kinase C
Correlazione tra composizione acidica del diacilglicerolo e attivazione della proteina cinasi C in colture di cardiomiociti di ratto.