Effects of interleukin-1 (IL-1) on postsurgical macrophage secretion of protease and protease inhibitor activities.
Although peritoneal macrophages secrete a variety of inflammatory mediators and proteases during postsurgical repair of the peritoneum, regulation of this secretion is poorly understood. Here, the responsivity of peritoneal macrophages to interleukin-1 (IL-1) stimulation in vitro, measured by the secretion of protease and protease inhibitor activities, was evaluated as a function of postsurgical time. Macrophages were harvested at various times after peritoneal sidewall abrasion, isolated by discontinuous density centrifugation and cultured with varying concentrations of IL-1. IL-1 increased the secretion of plasminogen activator (PA) activity by peritoneal macrophages in a concentration-dependent manner on postsurgical Days 0, 3, 10, and 14. Macrophages harvested on postsurgical Day 1 after surgery responded only to high concentration of IL-1, while on Days 5 and 7 all doses of IL-1 stimulate PA. On Days 7, 10, and 14 after surgery, the secretion of PA activity (after acid treatment) by postsurgical macrophages was generally high and increased with IL-1 treatment. The level of PA activity after inactivation of acid labile inhibitors (PAI) also increased in a dose-dependent manner on Days 0, 3, and 5. Although Day 1 macrophages expressed the highest PAI activity of all groups, they had relatively low responsivity to IL-1 with regards to PAI secretion. The level of elastase activity by postsurgical macrophages was lowest on Day 1, highest on Day 7, and decreased thereafter. All concentrations of IL-1 inhibited elastase activity of macrophages on Day 7.(ABSTRACT TRUNCATED AT 250 WORDS)
Kuraoka
S
S
Department of Obstetrics and Gynecology, University of Southern California School of Medicine, Los Angeles 90033.
Campeau
J D
JD
Rodgers
K E
KE
Nakamura
R M
RM
diZerega
G S
GS
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002478:Cells, Cultured; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D007813:Laparotomy; D008264:Macrophages / Q000201:enzymology*; D003012:Microbial Collagenase / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D010960:Plasminogen Activators / Q000378:metabolism*; D015849:Plasminogen Inactivators / Q000378:metabolism*; D011817:Rabbits; D013997:Time Factors; D014945:Wound Healing
D007375:Interleukin-1; D015849:Plasminogen Inactivators; D010960:Plasminogen Activators; D010196:Pancreatic Elastase; D003012:Microbial Collagenase