Cloning of a type I keratin from goldfish optic nerve: differential expression of keratins during regeneration.
We report the cDNA sequence and predicted amino acid sequence of a novel type I keratin, designated as GK50, and show that keratin expression in the goldfish optic nerve is highly complex. The GK50 protein is one of at least three type I keratins expressed in goldfish optic nerve based on both antibody reactivity and blot-binding to the type II keratin ON3. After optic nerve crush in situ hybridization shows a localized increase in GK50 mRNA expression in the crush zone. This is in contrast to ON3 mRNA which shows a localized increase that is limited to the proximal and distal margins of the crush zone, suggesting a diversity of keratin expression in different cell types of the goldfish optic nerve.
Druger
R K
RK
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794.
Levine
E M
EM
Glasgow
E
E
Jones
P S
PS
Schechter
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D006054:Goldfish; D007633:Keratins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009416:Nerve Regeneration; D009900:Optic Nerve / Q000737:chemistry* / Q000648:ultrastructure; D011485:Protein Binding; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid
D012333:RNA, Messenger; D007633:Keratins; D004247:DNA