[Preparation and regeneration of protoplasts of the strain Streptomyces hygroscopicus 155].
Protoplasts of Streptomyces hygroscopicus 155 producing nigericin, a polyether antibiotic, were prepared. Conditions for protoplasting and optimal concentrations of lysozyme and glycine, and the age of mycelium were studied. With the method of the experiment design, concentrations of four components: CaCl2, MgCl2, KH2PO4 and TES buffer in the regeneration medium were determined. The levels of CaCl2 and TES buffer proved to be the most important parameters.
Khristova
K R
KR
Petkova
V T
VT
Danilenko
V N
VN
Ivanova
I V
IV
Bakalov
B V
BV
D003470:Culture Media; D005998:Glycine / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D009550:Nigericin / Q000096:biosynthesis*; D011523:Protoplasts / Q000378:metabolism*; D012038:Regeneration / Q000502:physiology*; D013302:Streptomyces / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure
D003470:Culture Media; D009113:Muramidase; D009550:Nigericin; D005998:Glycine
Poluchenie i regeneratsiia protoplastov shtamma Streptomyces hygroscopicus 155.