Beta-glucosidase of a white-rot fungus Trametes gibbosa.
Extracellular beta-glucosidase was purified from a white-rot fungus, Trametes gibbosa by 50% ammonium sulphate saturation and Sephadex G-100 column chromatography. It showed maximum activity towards p-nitrophenyl- beta-D- glucopyranoside (pNpG). The pH optimum was 3.5. Temperature optimum was 40 degrees C but shifted to 50 degrees C on preincubation with pNpG. Hg2+, Fe3+ and Cu2+ strongly inhibited the activity. The enzyme was competitively inhibited by glucose with a Ki of 5.2 mM. The apparent molecular mass as determined by gel filtration chromatography was 640 kDa.
Bhattacharjee
B
B
Department of Botany, Visva-Bharati University, Santiniketan.
Roy
A
A
Majumder
A L
AL
D000645:Ammonium Sulfate / Q000737:chemistry; D001487:Basidiomycota / Q000201:enzymology*; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D002850:Chromatography, Gel; D003300:Copper / Q000494:pharmacology; D003470:Culture Media; D005290:Ferric Compounds / Q000494:pharmacology; D005960:Glucosides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008628:Mercury / Q000494:pharmacology; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D001617:beta-Glucosidase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D003470:Culture Media; D005290:Ferric Compounds; D005960:Glucosides; C025193:4-nitrophenyl beta-D-glucoside; D003300:Copper; D001617:beta-Glucosidase; D008628:Mercury; D002266:Carboxymethylcellulose Sodium; D000645:Ammonium Sulfate