Study of the substructure of the Morgagni and Brunescens cataract with the TAO non-coating technique. Part 2: Brunescens cataract.
Lens tissue from a Brunescens cataract was prepared for SEM study by prefixation with glutaraldehyde and postfixation with the tannic acid/arginine/OsO4 combination; for TEM study the material was prefixed with glutaraldehyde, postfixed with OsO4/K4Fe(CN)6 and poststained with uranyl acetate/lead citrate. At low magnification, in contrast to the Morgagni cataract, no difference could be seen between the lens fibres in the cortical and nuclear areas. Morphologically, the destruction of the ball and socket system and the development of holes and spherical structures was striking. The latter appeared to have a thin coating and, after fracture, were either empty or showed remnants of material resembling membranes. In sections of the cataractous material, larger vacuoles containing smaller spheres were indistinctly visible.
Jongebloed
W L
WL
Histology and Cell Biology Laboratorium, University of Groningen, The Netherlands.
Kalicharan
D
D
Los
L I
LI
Worst
J G
JG
D002386:Cataract / Q000473:pathology*; D005404:Fixatives; D006801:Humans; D007907:Lens Nucleus, Crystalline / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D017348:Microscopy, Electron, Scanning Transmission; D016707:Tissue Fixation / Q000379:methods*
D005404:Fixatives