Matrix-assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared.
A number of different matrices have been tested and compared for ultraviolet and infrared (UV and IR) matrix-assisted laser desorption/ionization (MALDI) of oligodeoxyribonucleotides and mixtures thereof, as well as ribonucleic acids (tRNA from yeast and rRNA from E. coli). A new technique for removing alkali cations from nucleic acid samples during sample preparation on the sample support is demonstrated. The amount of oligonucleotide sample consumed during a typical measurement in IR-MALDI-MS was determined.
Nordhoff
E
E
Institute for Medical Physics, University of Münster, Germany.
Ingendoh
A
A
Cramer
R
R
Overberg
A
A
Stahl
B
B
Karas
M
M
Hillenkamp
F
F
Crain
P F
PF
D000804:Angiotensin II / Q000032:analysis; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000737:chemistry; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D012313:RNA / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D009838:Oligodeoxyribonucleotides; D012335:RNA, Ribosomal; D000804:Angiotensin II; D012313:RNA; D012343:RNA, Transfer