Comparative evaluation of the MB-Check system for recovery of mycobacteria from clinical specimens.
The performance of the MB-Check system (Roche) in the recovery of mycobacteria from clinical specimens was compared with that of the Bactec radiometric system and conventional culture on Löwenstein-Jensen medium. A total of 1,582 clinical specimens were used for the study; 74 yielded mycobacteria. Organisms of the Mycobacterium tuberculosis and Mycobacterium avium complexes accounted for the majority of isolates. The rate of recovery of all mycobacteria with the Bactec system was 91.9%, with the MB-Check system 79.7% and on Löwenstein-Jensen medium only 54.1%. Combination of the Bactec and MB-Check systems allowed recovery of 100% of isolates. The Bactec system showed a faster detection time than the other two methods.
Piersimoni
C
C
Department of Clinical Microbiology, General Hospital Umberto I-Torrette, Ancona, Italy.
Morbiducci
V
V
De Sio
G
G
Scalise
G
G
D001431:Bacteriological Techniques / Q000295:instrumentation*; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D009161:Mycobacterium / Q000302:isolation & purification*
D003470:Culture Media