Antibody-mediated neutralization of human papillomavirus type 11 (HPV-11) infection in the nude mouse: detection of HPV-11 mRNAs.
The nu/nu mouse xenograft is the only experimental system permitting the growth of human papillomaviruses (HPV). Previous studies demonstrating inhibition of HPV-11 infection by antibodies against HPV-11 virions have used indirect markers of infection, such as graft size and histopathologic features. The presence of HPV-11 mRNAs was used as a direct marker of infection: Infectious HPV-11 was incubated with rabbit serum raised against purified HPV-11 virions or with the corresponding preimmune serum (controls) before use in the mouse xenograft model, and HPV-11 mRNAs were detected by a method using reverse transcription and amplification by polymerase chain reaction. Graft size, histopathologic features, and the presence of capsid antigen were also assessed. Six weeks after infection, 1 of 23 grafts in the test group contained HPV-11 mRNAs compared with 19 of 20 controls (P less than .001). Therefore, antibody-mediated inhibition of infection by HPV-11 leads to blockade of genomic expression and is thus consistent with active prevention of viral penetration, that is, neutralization.
Bonnez
W
W
Department of Medicine, University of Rochester School of Medicine and Dentistry, New York.
Rose
R C
RC
Reichman
R C
RC
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D009500:Neutralization Tests; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D011897:Random Allocation; D014412:Tumor Virus Infections / Q000276:immunology*; D014771:Virion / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D007106:Immune Sera; D012333:RNA, Messenger; D012367:RNA, Viral