title: A freeze-fracture study on the effect of neopybuthrin on the intestinal epithelial cells of Tilapia nilotica.
abstract: Freeze-fracture replicas of the plasma membrane and tight junctions (Tj) of intestinal epithelial cells were studied in Tilapia nilotica fish exposed to the pyrethroid insecticide, neopybuthrin. Exposing fishes to different repeated concentrations of 1/2 LC50 of neopybuthrin caused a significant decrease in the population density of IMPs in P- and E-faces. Tight junctions were also affected by neopybuthrin treatment. They appeared fragmented and discontinued, and their strands were fewer in number as compared with controls. Since the structure and number of Tj are major determinants of epithelial permeability, it is postulated that neopybuthrin treatment may affect the intestinal permeability of T. nilotica.
authors.affiliation: Zoology Dept., Faculty of Science, Menoufia University, Shebin El-kom, Egypt.
authors.forename: S A
authors.initials: SA
authors.lastname: Sakr
authors.forename: S A
authors.initials: SA
authors.lastname: Gabr
mesh_terms: D000818:Animals; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D005614:Freeze Fracturing; D007306:Insecticides / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000648:ultrastructure; D011722:Pyrethrins / Q000494:pharmacology*; D017210:Tilapia
chemical_list: D007306:Insecticides; D011722:Pyrethrins; C075628:neopybuthrin