[Microfluorimetric research on the erythroblastic islands and macrophages of the bone marrow].
A microfluorimetric system was used to study rat bone marrow erythroblastic islands (EI) and macrophages. Measurement of fluorescence from acridine-orange-treated cells was performed on the microscope LUMAM-E3 (LOMO, Leningrad) at 530-550 and 630-650 nm. The intensity of fluorescence in 530-550 nm range depended on the intensity of proliferative processes in EI, that at 630-650 nm was associated with activity of lysosomal apparatus of EI macrophages. Erythroblast amplification in EI is parallel both to enhancement of fluorescence intensity at 530-550 nm and 630-650 nm. Intensity of fluorescence of different bone marrow macrophages was evaluated. It is suggested that a part of bone marrow macrophages has high affinity to erythroid tissue.
Rassokhin
A G
AG
Pochinskiĭ
A G
AG
Liannoĭ
K V
KV
Ovchinnikov
G A
GA
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D003592:Cytophotometry; D004900:Erythroblasts / Q000166:cytology* / Q000187:drug effects; D004920:Erythropoiesis / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D008297:Male; D008856:Microscopy, Fluorescence; D051381:Rats; D017208:Rats, Wistar
D004921:Erythropoietin
Mikrofliuorimetricheskoe issledovanie éritroblasticheskikh ostrovkov i makrofagov kostnogo mozga.