Specific insulin binding by, and imprintability of, the nuclear membrane of Tetrahymena.
Cellular nuclei isolated from Tetrahymena were able to bind FITC-labelled insulin, and pretreatment with different concentrations of unlabelled insulin accounted for a decrease in FITC-insulin binding of the nuclear membrane. This strongly suggests an association of specific insulin receptors with the nuclear envelope of Tetrahymena. Nuclei isolated 1 week after primary exposure of Tetrahymena cells to insulin showed a 50% binding increase over the controls. This supports the implication that hormonal (insulin) imprinting also involves the receptors of the nuclear membrane of the imprinted cells.
Hegyesi
H
H
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Csaba
G
G
D000818:Animals; D007328:Insulin / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism*; D011972:Receptor, Insulin / Q000187:drug effects / Q000378:metabolism*; D013769:Tetrahymena pyriformis / Q000378:metabolism*
D007328:Insulin; D011972:Receptor, Insulin