[The ultrastructure of the centrosome in cytoplasts and its alteration under the action of ouabain].
It has been shown that after enucleation of the PE cells with cytochalasin D the centrioles remain in approximately 80% of cytoplasts. Some cytoplasts contain only single centriole, either a mother (active) of a daughter (inactive) one. 20% cytoplasts have no centrioles. 2h after enucleation the centrosome structure in the cytoplasts did not differ from that in normal cells. 14-16 h after enucleation in many cytoplasts large secondary lysosomes and lipid droplets appeared around the centrosome. At this time in some cytoplasts in the centrosome we observed free microtubule convergence foci. 14-16 h after the enucleation, some cytoplasts have doubling centrioles. Under the influence of ouabain (30 min), the number of active centrioles oriented perpendicularly to the substrate plane in the cytoplasts increased. We suggest that the preferentially perpendicular orientation of centrioles to the substrate plane does not depend on the nuclear activity.
Gorgidze
L A
LA
Vorob'ev
I A
IA
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects / Q000648:ultrastructure; D015638:Cytochalasin D / Q000494:pharmacology; D003593:Cytoplasm / Q000187:drug effects* / Q000648:ultrastructure; D004622:Embryo, Mammalian; D007668:Kidney; D008854:Microscopy, Electron / Q000379:methods; D010042:Ouabain / Q000494:pharmacology*; D013552:Swine; D013997:Time Factors
D015638:Cytochalasin D; D010042:Ouabain
Ul'trastruktura tsentrosomy v tsitoplastakh i ee izmenenie pod deĭstviem uabaina.