Modulating luminol-dependent chemiluminescence of neutrophils by flavones.
The effect of 14 flavones on luminol-dependent chemiluminescence of neutrophils was studied in vitro. Chemiluminescence was used in this study as an indicator for the production of a reactive oxygen species by neutrophils, stimulated by phorbol myristate acetate. While flavone-8-acetic acid, and most of the compounds tested, inhibited chemiluminescence, flavone and its 5-hydroxy-7-methoxy derivatives enhanced it by up to 150%. The most active inhibitors of photon emission were the glycosides. These results indicate that lipophilicity and some structural determinants modulate the chemiluminescent capacity of neutrophils.
Krol
W
W
Department of Microbiology, Silesian School of Medicine, Zabrze-Rokitnica, Poland.
Shani
J
J
Czuba
Z
Z
Scheller
S
S
D000818:Animals; D005419:Flavonoids / Q000494:pharmacology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D013329:Structure-Activity Relationship
D005419:Flavonoids; D008165:Luminol