title: [Mixed-function oxidase induction as a test for the biological monitoring of water: limitations and prospects].
abstract: The induction in fish liver of some enzyme activities, and typically of microsomal mixed-function oxidases (MFO), provides the earliest biological warning signal of exposure to pollutants. Our studies provided evidence that the basal levels of cytochrome P-450 and specific MFO activities, such as arylhydrocarbon hydroxylase (AHH) and ethoxyresorufin deethylase (EROD), were strongly influenced by the diet in freshwater fish (Oncorhynchus mykiss). The response of fish liver to a known enzyme inducer, i.e., beta-naphthoflavone, was also affected by the diet, which therefore should be carefully controlled in laboratory studies. Under field conditions MFO activities were significantly enhanced in the liver of O. mykiss kept in polluted river water as well as in the liver of the seawater fish Diplodus annularis collected from a polluted harbour area, as compared to specimens of the same species collected from an unpolluted reference area.
authors.affiliation: Istituto di Zoologia, Facoltà di Scienze MFN, Università di Genova.
authors.forename: A
authors.initials: A
authors.lastname: Arillo
authors.forename: M
authors.initials: M
authors.lastname: Bagnasco
authors.forename: C
authors.initials: C
authors.lastname: Bennicelli
authors.forename: F
authors.initials: F
authors.lastname: Melodia
authors.forename: L
authors.initials: L
authors.lastname: Viganò
mesh_terms: D000821:Animal Feed; D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods*; D004784:Environmental Monitoring; D004790:Enzyme Induction / Q000187:drug effects; D005399:Fishes / Q000378:metabolism*; D005508:Food Deprivation; D005618:Fresh Water; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D012474:Salmon / Q000378:metabolism; D012623:Seawater; D013045:Species Specificity; D014876:Water Pollution; D019324:beta-Naphthoflavone
chemical_list: D001571:Benzoflavones; D019324:beta-Naphthoflavone; D006899:Mixed Function Oxygenases
vernacular_title: Induzione delle ossigenasi a funzione mista come strumento di monitoraggio biologico delle acque: limiti e prospettive.