title: Fibroblastic growth and attachment on hydroxyapatite-coated titanium surfaces following the use of various detoxification modalities. Part II: Contaminated hydroxyapatite.
abstract: This study evaluated the ability of various chemotherapeutic and mechanical modalities to detoxify endotoxin-contaminated hydroxyapatite-coated dental implant surfaces as determined by the early attachment and growth of human gingival fibroblasts. Hydroxyapatite-coated test strips were contaminated with purified outer membranes of Escherichia coli and treated with citric acid, hydrogen peroxide, stannous fluoride, chlorhexidine gluconate, tetracycline HCl, polymyxin B, a plastic sonic scaler tip, or left untreated (contaminated and sterile controls). Human gingival fibroblasts were then seeded onto the test strips and incubated for 48 hours. The citric acid-treated strips showed greater cell growth than the other treatments. The plastic sonic scaler tip and the polymyxin B-treated samples exhibited greater cell coverage than the sterile control specimens. The use of citric acid and/or a modified plastic sonic scaler tip may be a valuable adjunct when surgical repair of an ailing hydroxyapatite-coated dental implant is contemplated.
authors.affiliation: School of Dentistry, University of California, San Francisco.
authors.forename: M H
authors.initials: MH
authors.lastname: Zablotsky
authors.forename: E E
authors.initials: EE
authors.lastname: Wittrig
authors.forename: D L
authors.initials: DL
authors.lastname: Diedrich
authors.forename: D L
authors.initials: DL
authors.lastname: Layman
authors.forename: R M
authors.initials: RM
authors.lastname: Meffert
mesh_terms: D002448:Cell Adhesion; D002710:Chlorhexidine / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D003666:Decontamination; D015921:Dental Implants; D004731:Endotoxins; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006882:Hydroxyapatites; D011112:Polymyxin B / Q000494:pharmacology*; D014002:Tin Fluorides / Q000494:pharmacology; D014465:Ultrasonics
chemical_list: D002951:Citrates; D015921:Dental Implants; D004731:Endotoxins; D006882:Hydroxyapatites; D014002:Tin Fluorides; D019343:Citric Acid; D006861:Hydrogen Peroxide; D011112:Polymyxin B; D002710:Chlorhexidine