Characterization of the promoter region of the murine fibroblast growth factor receptor 1 gene.
To obtain some clue for the regulatory mechanism by which fibroblast growth factor (FGF) receptor 1 (FGFR 1) gene is expressed, we have cloned the promoter region of this gene from genomic library of mouse FGF-responsive cell lines. The genomic clone isolated here includes the FGFR 1 gene from position -868 to +697 relative to transcription initiation site. Sequence analysis reveals the presence of various consensus sequences for the binding sites of transcriptional factors such as SP 1, GCF, Oct-I, AP 1 and AP 2, but the absence of TATA and CAAT sequence motif. The transfection of this promoter-CAT constructs into NIH 3T3 cells demonstrates its promoter activity which is at least located between base -106 and +104.
Saito
H
H
Department of Internal Medicine III, Osaka University Hospital, Japan.
Kouhara
H
H
Kasayama
S
S
Kishimoto
T
T
Sato
B
B
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004252:DNA Mutational Analysis; D005346:Fibroblast Growth Factors / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics*; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins; D016385:TATA Box; D014158:Transcription, Genetic; D014162:Transfection
D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors; D015500:Chloramphenicol O-Acetyltransferase