title: Oogenesis, fertilisation and early embryonic development in rats. I: Dose-dependent effects of pregnant mare serum gonadotrophins.
abstract: Five hundred and eight mature female Wistar rats divided into 35 different groups were stimulated with pregnant mare serum gonadotrophins (PMSG) (0, 5, 10, 20 & 40 IU) at the late diestrus stage to induce multiple follicular development. No chorionic gonadotrophin (CG) was used for ovulation induction. The quality of oocytes and their in vitro fertilisability, quality of Day 2-embryos, viability of pregnancy and status of fetuses on Day 14 of gestation and status of embryos retrieved on Day 2, 3, 4 and 5 of pregnancy in different subgroups of rats were examined. Results showed that more oocytes and embryos fertilised in in vivo were retrieved from rats supraphysiologically stimulated with 20 IU of PMSG. However, concurrent with the larger number, higher proportions of abnormal oocytes and embryos were found. High doses of PMSG caused lower in vitro fertilisability of oocytes and greater degrees of embryonic degeneration. Although, the number of oocytes and Day 2-embryos were higher in the 20PMGS dose group, the pregnancy rate was significantly reduced to 27%. In the 40PMSG group no viable pregnancy was noted. Most embryo demise occurred by day 3-5 of pregnancy, probably within the oviducts and before the implantation stage. In rats supraphysiologically stimulated with 20 and 40 IU of PMSG, the number of morphologically normal looking embryos was greatly reduced by Day 3-5 of pregnancy. In the 40PMSG group, there were no embryos retrieved by Day 4 and 5.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Obstetrics & Gynaecology, National University Hospital, National University of Singapore.
authors.forename: H H
authors.initials: HH
authors.lastname: Goh
authors.forename: X F
authors.initials: XF
authors.lastname: Yang
authors.forename: C F
authors.initials: CF
authors.lastname: Tain
authors.forename: L P
authors.initials: LP
authors.lastname: Liew
authors.forename: S S
authors.initials: SS
authors.lastname: Ratnam
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000187:drug effects; D005328:Fetal Viability / Q000187:drug effects; D006064:Gonadotropins, Equine / Q000008:administration & dosage*; D009865:Oocytes / Q000187:drug effects; D009866:Oogenesis / Q000187:drug effects*; D011247:Pregnancy; D011272:Pregnancy, Multiple; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D006064:Gonadotropins, Equine