HS-142-1, a novel non-peptide ANP antagonist, blocks the cyclic GMP production elicited by natriuretic peptides in PC12 and NG108-15 cells.
HS-142-1 is a novel non-peptide antagonist for atrial natriuretic peptide (ANP) receptor. The effect of HS-142-1 on the cyclic GMP production elicited by natriuretic peptides in neuronal cell lines, PC12 and NG108-15 was examined. Natriuretic peptides such as ANP, brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP) enhanced cyclic GMP production in a dose-dependent manner. HS-142-1 inhibited cyclic GMP accumulation elicited by natriuretic peptides in a dose-dependent fashion in both cells. The results suggest that HS-142-1 will be an important tool for identification and understanding of the mechanisms by which natriuretic peptides act in nervous systems.
Toki
S
S
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
Morishita
Y
Y
Sano
T
T
Matsuda
Y
Y
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D002460:Cell Line; D006152:Cyclic GMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005910:Glioma; D006822:Hybrid Cells / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma; D016716:PC12 Cells; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats
C072551:HS 142-1; D011134:Polysaccharides; D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP