title: The herpes simplex virus type 1 tegument protein VP22 is encoded by gene UL49.
abstract: VP22 is a major tegument protein of herpes simplex virus type 1 and is highly phosphorylated in the infected cell. Indirect evidence exists to suggest that it is encoded by gene UL49, present in the BamHI F fragment of the genome. Using the polymerase chain reaction we have cloned the UL49 open reading frame into a mammalian expression vector under the control of the human cytomegalovirus immediate early gene promoter. After transfection into COS-7 cells expression of the gene product was detected by means of Western blotting and immunofluorescence. The results clearly indicate that the protein encoded by UL49 is VP22, and that in transfected cells it appears to have characteristics similar to those of the protein synthesized in infected cells.
authors.affiliation: Department of Microbiology, University of Leeds, U.K.
authors.forename: G D
authors.initials: GD
authors.lastname: Elliott
authors.forename: D M
authors.initials: DM
authors.lastname: Meredith
mesh_terms: D002213:Capsid / Q000235:genetics*; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics*; D005822:Genetic Vectors; D006561:Herpes Simplex / Q000235:genetics; D011401:Promoter Regions, Genetic; D018139:Simplexvirus / Q000235:genetics*; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins
chemical_list: D014764:Viral Proteins; D015678:Viral Structural Proteins; C073413:herpes simplex virus type 1 protein VP22