Stellate cell inhibition of Purkinje cells in the turtle cerebellum in vitro.
1. The stellate cell-mediated inhibition of Purkinje cells was studied by intracellular recordings in an in vitro slice preparation of the turtle cerebellar cortex. A graded inhibitory postsynaptic potential (IPSP) was recorded in Purkinje cells upon stimulation of the parallel fibre-stellate cell pathway. 2. The IPSP was abolished by bicuculline, and had a reversal potential around -75 mV, consistent with a GABAA receptor-operated Cl- conductance dominating the response investigated here. 3. Paired recordings from synaptically coupled stellate cells and Purkinje cells demonstrated that the inhibitory input from a single stellate cell is sufficient to reduce the firing in a Purkinje cell. 4. The extracellular-evoked IPSP interacted with the active postsynaptic membrane properties in the Purkinje cell. Interaction with both the Na+ plateau and the IA prolonged the responses to an IPSP, making the net effect of the inhibitory response dependent on the membrane potential in each postsynaptic neurone. 5. A precisely timed IPSP was particularly efficient in reducing dendritic Ca2+ influx. 6. The voltage-dependent Ca2+ component of a climbing fibre response (CFR) as well as of a parallel fibre (PF) input was reduced by the IPSP. 7. It is suggested that Ca2+ spike-mediated reduction in Purkinje cell excitability may be prevented by the stellate cell IPSP-mediated reduction in Ca2+ influx.
Midtgaard
J
J
Institute of Neurophysiology, University of Copenhagen, Denmark.
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D066298:In Vitro Techniques; D007395:Interneurons / Q000502:physiology*; D011689:Purkinje Cells / Q000502:physiology*; D013233:Stellate Ganglion / Q000166:cytology / Q000502:physiology*; D014426:Turtles / Q000502:physiology*
D002118:Calcium; D001640:Bicuculline