[Identification and differentiation of the human herpes virus group using the PCR method].
Six kinds of human herpes viruses have been identified and classified on the basis of structure and characteristics. We studied the identification and classification of these types using PCR to amplify the virus-specific DNA sequences. This method showed higher sensitivity than the conventional method of virus isolation and culture for HSV and CMV detection. For each positive control, the viral DNA was amplified only when the complementary primers themselves were used. PCR apparently detects only the activated virus, because normal controls were negative when this method was used. Therefore, the present method is thought to closely reflect viral activation and infectious diseases in patients with latent infections.
Kawaguchi
R
R
Genetic Research Laboratory, SRL, Inc., Hachioji.
Shibuya
Y
Y
Ogasa
U
U
Kosuda
O
O
Hikiji
K
K
Ishii
K
K
D001483:Base Sequence; D006564:Herpesviridae / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction