title: Germline intronic and exonic mutations in the Wilms' tumour gene (WT1) affecting urogenital development.
abstract: Denys-Drash syndrome is a rare human developmental disorder affecting the urogenital system and leading to renal failure, intersex disorders and Wilms' tumour. In this report, four individuals with this syndrome are described carrying germline point mutations in the Wilms' tumour suppressor gene, WT1. Three of these mutations were in the zinc finger domains of WT1. The fourth occurred within intron 9, preventing splicing at one of the alternatively chosen splice donor sites of exon 9 when assayed in vitro. These results provide genetic evidence for distinct functional roles of the WT1 isoforms in urogenital development.
authors.affiliation: Division of Pediatric Endocrinology, Children's Memorial Hospital, Chicago, Illinois 60614.
authors.forename: W
authors.initials: W
authors.lastname: Bruening
authors.forename: N
authors.initials: N
authors.lastname: Bardeesy
authors.forename: B L
authors.initials: BL
authors.lastname: Silverman
authors.forename: R A
authors.initials: RA
authors.lastname: Cohn
authors.forename: G A
authors.initials: GA
authors.lastname: Machin
authors.forename: A J
authors.initials: AJ
authors.lastname: Aronson
authors.forename: D
authors.initials: D
authors.lastname: Housman
authors.forename: J
authors.initials: J
authors.lastname: Pelletier
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004252:DNA Mutational Analysis; D005091:Exons; D005260:Female; D016162:Genes, Wilms Tumor; D005838:Genotype; D005854:Germ Cells; D006801:Humans; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D013577:Syndrome; D014564:Urogenital Abnormalities; D014566:Urogenital System / Q000254:growth & development
chemical_list: D004247:DNA