Two-site pan-species monoclonal antibody ELISA for detection of blood stage malaria antigen.
A two-site pan-species monoclonal antibody sandwich ELISA (MAb-MAb ELISA) was developed to detect both Plasmodium vivax and P. falciparum antigens in whole blood impregnated on filter paper. In this assay, the plates were coated with pan-species MAb 3F9 and another pan-species MAb M26-32 conjugated with alkaline phosphatase was used for detection of bound antigen. The sensitivity of this assay was 5, 10 and 10 parasites per 10(6) erythrocytes for cultured P. falciparum, patient-derived P. vivax and P. falciparum, respectively. The coincidence rates for this assay were 93% (92/99) with healthy individuals and 93% (42/45) with microscopically confirmed vivax malaria cases. After two weeks treatment, 77.7% (14/18) of vivax malaria were still positive by this assay but with diminished level of reactivities [corrected].
Gao
Q
Q
Jiangsu Institute of Parasitic Diseases, Wuxi, People's Republic of China.
Tharavanij
S
S
Khusmith
S
S
Wang
L Q
LQ
Looareesuwan
S
S
Zhang
W G
WG
D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000276:immunology; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000276:immunology; D016789:Plasmodium cynomolgi / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D010966:Plasmodium vivax / Q000276:immunology*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan