Simian virus 40 (SV40) small t antigen inhibits SV40 DNA replication in vitro.
We describe a biochemical function of simian virus 40 small t antigen, the inhibition of simian virus 40 large T antigen-mediated viral DNA replication in an in vitro replication system. Our results suggest that in this system, small t antigen prevents protein phosphatase 2A-mediated activation of large T antigen.
Carbone
M
M
Section on Viruses and Cellular Biology, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
Hauser
J
J
Carty
M P
MP
Rundell
K
K
Dixon
K
K
Levine
A S
AS
D000952:Antigens, Polyomavirus Transforming / Q000502:physiology*; D002472:Cell Transformation, Viral; D002474:Cell-Free System; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D054648:Protein Phosphatase 2; D013539:Simian virus 40 / Q000235:genetics*; D014779:Virus Replication
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2