Glycerol shock treatment facilitates purification of herpes simplex virus.
We describe an improved method for purification of herpes simplex virus. The primary improvement is the use of glycerol shock treatment to lyse infected cells. This is a rapid and simple technique that efficiently extracts the cytoplasm from the nucleus, while preserving nuclear integrity. Nearly 100% cell lysis is achieved by this method, and cytoplasmic virus yield is optimal. Other improvements to increase virus yield and purity and to economize on equipment are described.
Sarmiento
M
M
Department of Veterinary Medicine and Surgery, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Batterson
W W
WW
D000818:Animals; D003593:Cytoplasm / Q000382:microbiology; D005990:Glycerol / Q000494:pharmacology*; D007038:Hypotonic Solutions; D018139:Simplexvirus / Q000302:isolation & purification*; D014709:Vero Cells / Q000187:drug effects; D014764:Viral Proteins / Q000032:analysis
D007038:Hypotonic Solutions; D014764:Viral Proteins; D005990:Glycerol