Liposomes and conjugate vaccines for antigen delivery and induction of mucosal immune responses.
In this brief review, emphasis was placed on the effectiveness of liposomes as carriers/vehicles of soluble antigens and as adjuvants for use in oral vaccine development. Evidence was provided that oral administration of antigen in liposomes resulted in a mucosal response which was higher than that obtained when the oral vaccine consisted of antigen alone. Specific mucosal responses were enhanced by incorporating lipophilic MDP into the antigen/liposome vaccines. Antigens shown to be effective in inducing a protective mucosal response when given in an oral liposome vaccine were anti-idiotypic antibodies, purified S. mutans GTF, CHO and Ag I/II. Evidence is also provided that CTB may be an effective oral adjuvant when coupled to proteins or peptides by either chemical or genetic methods. Further studies, however, will be required to characterize the effectiveness and safety of CTB in conjugate vaccines for inducing specific mucosal responses and to develop practical means to prepare oral liposome vaccines for use in humans.
Michalek
S M
SM
Department of Microbiology, School of Dentistry, University of Alabama, Birmingham 35294.
Childers
N K
NK
Katz
J
J
Dertzbaugh
M
M
Zhang
S
S
Russell
M W
MW
Macrina
F L
FL
Jackson
S
S
Mestecky
J
J
D000818:Animals; D000956:Antigens, Viral / Q000008:administration & dosage* / Q000276:immunology*; D004337:Drug Carriers; D006801:Humans; D008081:Liposomes; D009061:Mouth Mucosa / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology*
D000956:Antigens, Viral; D004337:Drug Carriers; D008081:Liposomes; D014765:Viral Vaccines