title: [The use of flow cytofluorometry for determining antisperm antibodies by sperm immobilization and sperm toxicity methods].
abstract: During antisperm antibody detection by conventional methods of immobilization and cytotoxicity investigators used flow cytometry to detect dead and/or immovable sperm cells. It was shown that after incubation of sperm cells with low antiserum dilution immovable spermatozoa are still living. Evidently, cytotoxicity test in this case brings about false-negative results. Therefore, flow cytometry will be a success for ASAB detection through immobilization test.
authors.forename: M N
authors.initials: MN
authors.lastname: Nikolaeva
authors.forename: M Sh
authors.initials: MSh
authors.lastname: Verbitskiĭ
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood*; D016923:Cell Death / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D011817:Rabbits; D013081:Sperm Motility / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*
chemical_list: D000906:Antibodies
vernacular_title: Ispol'zovanie protochnoĭ tsitofliuorometrii dlia opredeleniia antispermal'nykh antitel metodami spermoimmobilizatsii i spermotoksichnosti.