Comparative studies of the carbohydrate of human gamma-crystallins from fetal and adult lenses with agglutinins.
Using gel chromatography of Sephadex G-75 superfine connected with Sephadex G-50 fine column, three human gamma-crystallins (gamma 1, gamma 2, gamma 3) could be obtained. Seven agglutinins (LCA, SBA, DBA, PNA, BSL, RCA and UEA) were used to detect the sugar of sub-gamma-crystallins, which had been transferred to nitrocellulose membrane and finally stained with ABC reagents and the substrate of HPR. These results suggested that gamma 2- and gamma 3-crystallins contain sugar, but gamma 1-crystallin has no sugar. There is a decrease of carbohydrate of gamma 2 and gamma 3 as the development of the lens (esp. the former). It was postulated that in gamma-crystallins (gamma 2, gamma 3) the sugar components helped stabilizing the molecular structure (folding polypeptide, prevent protein breaking). The sugar components decreased with age by the post-translation modification.
Wu
K
K
National Ophthalmological Laboratories, Ministry of Public Health, China.
Li
S
S
Chen
S
S
Guo
H
H
D000328:Adult; D000367:Age Factors; D000373:Agglutinins; D002241:Carbohydrates / Q000032:analysis*; D002850:Chromatography, Gel; D003459:Crystallins / Q000737:chemistry*; D005333:Fetus; D006801:Humans; D007908:Lens, Crystalline / Q000737:chemistry*
D000373:Agglutinins; D002241:Carbohydrates; D003459:Crystallins