A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification.
"Chelex 100" chelating resin has been previously proposed for the rapid extraction of human DNA for polymerase chain reaction. Protocols are given for the rapid extraction of bacterial and viral DNA from cultures or clinical samples. The DNA samples obtained were suitable for use in polymerase chain reaction.
de Lamballerie
X
X
Laboratoire de Microbiologie et d'Hygiène Hospitalières, Hôpital Salvator, Marseille, France.
Zandotti
C
C
Vignoli
C
C
Bollet
C
C
de Micco
P
P
D002614:Chelating Agents; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005784:Gene Amplification; D006090:Gram-Negative Bacteria / Q000235:genetics*; D006094:Gram-Positive Bacteria / Q000235:genetics*; D066298:In Vitro Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D012117:Resins, Synthetic; D013292:Streptococcus agalactiae / Q000235:genetics
D002614:Chelating Agents; D004269:DNA, Bacterial; D012117:Resins, Synthetic; C024997:Chelex 100