title: Interaction of 2-deoxy-D-glucose and adenine with phosphate anion uptake in yeast.
abstract: The transport of inorganic phosphate anions into yeast cells (after preincubation with glucose, fructose or another metabolizable sugar, and in the presence of glucose) shows two kinetic components with half-saturation constants of 40 mumol/L and 2.4 mmol/L. The uptake was strikingly stimulated by 2-deoxy-D-glucose (2-dGlc) at lower concentrations but inhibited above 100 mmol/L. A similar stimulation was caused by adenine (0.01-1 mmol/L) and a very small one by uracil and inorganic sulfate. It is suggested that either a phosphorylation reaction accompanies the transport (2-dGlc) or that some compounds stimulate the H(+)-ATPase more than inorganic phosphate itself and thus increase its rate of transport.
authors.affiliation: Department of Membrane Transport, Czechoslovak Academy of Sciences, Prague.
authors.forename: A
authors.initials: A
authors.lastname: Kotyk
mesh_terms: D000225:Adenine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism*; D010766:Phosphorylation; D006180:Proton-Translocating ATPases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*
chemical_list: D010710:Phosphates; D000255:Adenosine Triphosphate; D003847:Deoxyglucose; D006180:Proton-Translocating ATPases; D000225:Adenine