An evaluation of adi agbon as a clearing agent in paraffin processing of embryonic tissues--preliminary report.
Adi agbon, an oily extract of the endosperm of the coconut fruit (Cocoa nucifera, L.) was investigated as a clearing agent for embryonic soft tissues. Paraffin sections stained with haematoxylin an eosin revealed normal tissue architecture and light microscopic details were easily identified. However, some tissue shrinkage was observed, particularly in the brain. As prepared for this study, this locally available compound is useful for routine work but appears unsuitable for quantitative histological studies.
Shokunbi
M T
MT
Department of Anatomy, University of Ibadan, Nigeria.
Oke
B O
BO
Prasada-Rao
P V
PV
Caxton-Martin
A E
AE
D000818:Animals; D003059:Cocos; D003584:Cytological Techniques / Q000592:standards*; D004622:Embryo, Mammalian / Q000166:cytology*; D005069:Evaluation Studies as Topic; D006416:Hematoxylin / Q000592:standards; D051379:Mice; D010232:Paraffin; D010936:Plant Extracts / Q000592:standards*
D010936:Plant Extracts; D010232:Paraffin; D006416:Hematoxylin