Subcellular distribution of differently glycosylated forms of active and inactive renin in rat kidney: effect of sodium depletion and captopril treatment.
Distribution of differently glycosylated forms of active and inactive renin was investigated in fractions prepared by discontinuous density gradient centrifugation of rat kidney cortex homogenate. Concanavalin A (con A) chromatography separated active and inactive renin into three differently glycosylated forms in both cytosol and granule fractions: a minor con A unbound form (I), a loosely bound form (II), and the most predominant tightly bound form (III). After a long-term stimulation of renin synthesis and secretion by sodium depletion and captopril treatment the relative proportion of active renins I and II in granules significantly increased, while that of active renin III decreased. The relative proportion of inactive renins I and II in granule compared to cytosol fractions was significantly higher and that of inactive renin III was lower. The results indicate that glycosylation might have some relation to the rate of sorting of prorenin into the granules, or of its processing within granules. Alternatively, it might influence the rate of inactive renin release via the constitutive pathway.
Jindra
A
A
2nd Department of Internal Medicine, Charles University, Praha, Czech Republic.
Entlicher
G
G
Sosna
B
B
Horký
K
K
D000818:Animals; D002216:Captopril / Q000494:pharmacology*; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D003208:Concanavalin A; D003594:Cytoplasmic Granules / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D006031:Glycosylation; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D012083:Renin / Q000302:isolation & purification / Q000378:metabolism*; D012964:Sodium / Q000008:administration & dosage*
D003208:Concanavalin A; D002216:Captopril; D012964:Sodium; D012083:Renin