Interaction of novel tris-intercalators with DNA. Spectrofluorometric studies.
Novel DNA binding ligands (1 and its stereoisomer 2) which contain three potentially intercalating units in a linear molecular skeleton were prepared. From a study of the displacement of ethidium bromide from several natural and synthetic polynucleotides, both compounds 1 and 2 were found to show an AT base-pair preference in the interaction with DNAs with 2 a slightly higher affinity for DNA. This result is in sharp contrast to that for acridine and anthraquinone, because these two compounds exhibit a GC-preference.
Takenaka
S
S
Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Japan.
Nishira
S
S
Kondo
H
H
Takagi
M
M
D004247:DNA / Q000737:chemistry*; D004996:Ethidium; D007364:Intercalating Agents / Q000737:chemistry*; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence
D007364:Intercalating Agents; D004247:DNA; D004996:Ethidium