Human brain lectin: a soluble lectin that binds actin.
A biotinylated probe was used for detection of endogenous ligands of a human brain lectin on blotted human brain soluble proteins. Of the various proteins from brain extract resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, five reacted with the biotinylated probe. After elimination of saccharidic moieties by periodic treatment of the same extract, a single band with Mr approximately 43,000 was recognized by the lectin. This band was identified as actin using an anti-actin antibody. These results were confirmed by binding of biotinylated lectin to purified actin.
Joubert
R
R
Laboratoire de Biochimie et Technologie des Protéines, Université Paris-Nord, Bobigny, France.
Caron
M
M
Avellana-Adalid
V
V
Mornet
D
D
Bladier
D
D
D000199:Actins / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D037102:Lectins / Q000378:metabolism*; D010504:Periodic Acid / Q000494:pharmacology; D012995:Solubility; D014020:Tissue Extracts / Q000378:metabolism
D000199:Actins; D037102:Lectins; D014020:Tissue Extracts; D010504:Periodic Acid; C009288:metaperiodate