[Effect of CCK receptor antagonists on plasma CCK bioassay].
Fasting and postprandial plasma CCK levels of 102 normal subjects were measured by bioassay with dispersed rat pancreatic acini. The reference values ranged from 0 to 4.2 pmol/L (CCK-8 equivalents) for fasting and from 1.1 to 13.5 pmol/L for postprandial state. There was no significant difference between male and female, or in different age groups. The effects of CCK receptor antagonists of 3 different categories on CCK bioactivity in plasma measured by the bioassay were investigated. L 364,718 (5 nmol/L), proglumide (1.0 mmol/L), or Bt2-cGMP (0.1 mmol/L) was either extracted by SEP-PAK C18 cartridges together with human plasma containing 8 pmol/L of CCK-8, or added into the plasma extracts before the assay. The CCK bioactivity was inhibited by all of the 3 CCK antagonists. The action of L364,718 could be eliminated by the procedure of plasma extraction, but not of proglumide or Bt2-cGMP. It was suggested that CCK bioassay can be used even if L364,718 was administered. However, CCK cannot be measured accurately if there are proglumide or Bt2-cGMP in the plasma.
Sun
G
G
Beijing Union Medical College Hospital.
Pan
G Z
GZ
Ma
F
F
Wen
S H
SH
Du
S F
SF
D000293:Adolescent; D000328:Adult; D000368:Aged; D001570:Benzodiazepinones / Q000494:pharmacology; D001681:Biological Assay; D002766:Cholecystokinin / Q000037:antagonists & inhibitors / Q000097:blood*; D020109:Devazepide; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011377:Proglumide / Q000494:pharmacology; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors*
D001570:Benzodiazepinones; D011949:Receptors, Cholecystokinin; D002766:Cholecystokinin; D011377:Proglumide; D020109:Devazepide