The immunomodulating preparation Ukrain does not induce anaphylactic sensitization in mice and guinea pigs.
The ability of Chelidonium majus L. alkaloids derivative Ukrain to induce an anaphylactic sensitization was tested on mice and guinea pigs. The levels of IgE antibody in the mouse sera, and IgG1a, IgG1b as well as IgE antibody levels in guinea pig sera, were evaluated by passive cutaneous anaphylaxis (PCA) tests. Ukrain alone or adsorbed on aluminium hydroxide gel (alum) introduced into BALB/c mice in several subcutaneous injections was unable to stimulate measurable anti-Ukrain IgE antibody response. Moreover, Ukrain introduced together with ovalbumin (OA) into mice in the course of immunization with OA induced lower anti-OA antibody response as compared to the response induced by OA alone. Ukrain adsorbed on alum and injected subcutaneously into guinea pigs did not induce measurable IgG1a, IgG1b and IgE antibody response. The present results suggest that the immunomodulating preparation Ukrain could be therapeutically safe at least as far its inability to induce anaphylaxis is concerned.
Wyczółkowska
J
J
Department of Biogenic Amines, Polish Academy of Sciences, Lódź, Poland.
Czuwaj
M
M
Maśliński
C
C
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000470:Alkaloids / Q000276:immunology*; D000707:Anaphylaxis / Q000276:immunology*; D000818:Animals; D001600:Berberine Alkaloids; D005260:Female; D006168:Guinea Pigs; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010617:Phenanthridines
D000276:Adjuvants, Immunologic; D000470:Alkaloids; D001600:Berberine Alkaloids; D007074:Immunoglobulin G; D010617:Phenanthridines; D007073:Immunoglobulin E; C060921:ukrain; D010047:Ovalbumin