title: Acute inhibition of lipid transport in rat intestinal lymph by ethanol administration.
abstract: To observe the effect of acute ethanol ingestion on stable lipid transport in intestinal lymph, three groups of mesenteric lymph fistula rats were prepared. In group A rats, lipid emulsion containing 120 mumol/hr of oleate (control meal) was infused intraduodenally for 8 hr. Group B rats were infused with another lipid emulsion, which had the same basic composition as the control meal but included 0.75 g/kg body weight/hr of ethanol (alcohol meal), for 8 hr. In group C, rats were infused with the control meal for the first 4 hr, then with the alcohol meal for the next 4 hr. Lymph flow increased just after the infusion of ethanol, that is, lymph flow in group B was significantly higher than that of group A at 1 and 2 hr, and lymph flow in group C was significantly higher than that of group A at 5 hr. In group A, lymphatic triglyceride (TG) output reached a plateau of more than 30 mumol/hr after 3-hr infusion. TG output in group B was significantly lower than that in group A from 2 hr to 8 hr and was two-thirds that of group A at the plateau level. In group C, TG output was the same as in group A until 5 hr, but decreased to two-thirds of that in group A at 6 hr, 2 hr after replacing the control meal with the alcohol meal. Lymphatic phospholipid output exhibited a tendency similar to that of TG output. Comparisons of group A vs. B and A vs. C clearly demonstrated an inhibitory effect of ethanol on stable lipid transport in intestinal lymph. A 2-hr infusion was enough for ethanol to exhibit its inhibitory effect. In conclusion, stable lymphatic transport of intestinal absorbed lipid was suppressed by acute ethanol ingestion.
authors.affiliation: Department of Public Health and Environmental Science, School of Medicine, Tokyo Medical and Dental University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Nakata
authors.forename: Y
authors.initials: Y
authors.lastname: Mótohashi
authors.forename: T
authors.initials: T
authors.lastname: Takano
mesh_terms: D000818:Animals; D000431:Ethanol / Q000008:administration & dosage / Q000009:adverse effects*; D007408:Intestinal Absorption / Q000187:drug effects*; D050356:Lipid Metabolism; D008055:Lipids / Q000008:administration & dosage / Q000302:isolation & purification; D008196:Lymph / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000302:isolation & purification; D051381:Rats; D013997:Time Factors; D014280:Triglycerides / Q000302:isolation & purification
chemical_list: D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides; D000431:Ethanol