title: Opposite effects of rough and gentle handling with repeated saline administration on c-fos mRNA expression in the rat brain.
abstract: The rough handling with repeated saline administration (1.2 ml/kg s.c. for 7 days) enhanced cortical c-fos mRNA expression in the rat brain after a single saline stimulation (1.2 ml/kg s.c.) due to increasing baseline c-fos mRNA levels, whereas the gentle handling with repeated saline administration declined c-fos mRNA expression after a single injection due to decreasing the baseline of c-fos mRNA levels. These two types of handling with the repeated injection led to diametrically opposite results on c-fos mRNA expression after a single stimulation. Neither two types of handling with repeated saline injections affected the net increment of c-fos mRNA induction after a single stimulation, therefore, the effects of handling with repeated treatment on c-fos mRNA expression might be independent of the effects of a single saline stimulation. The present study suggests that c-fos mRNA induction after a single stimulation might be affected by the types or intensities of handling and that care must be taken to estimate c-fos mRNA induction.
authors.affiliation: Department of Neuroscience, Okayama University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Asanuma
authors.forename: N
authors.initials: N
authors.lastname: Ogawa
authors.forename: H
authors.initials: H
authors.lastname: Hirata
authors.forename: H
authors.initials: H
authors.lastname: Chou
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Mori
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D003342:Corpus Striatum / Q000378:metabolism; D015870:Gene Expression; D016762:Genes, fos / Q000502:physiology*; D006234:Handling, Psychological; D007267:Injections; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000008:administration & dosage*
chemical_list: D012333:RNA, Messenger; D012965:Sodium Chloride