title: Differential regulation in the release of bioactive versus immunoactive gonadotropins from cultured rat pituitary cells by inhibin and androgens.
abstract: Using in vitro bioassays and radioimmunoassays, the authors examined the interactions between purified 32kD ovine inhibin, testosterone, and dihydrotestosterone on basal and GnRH-stimulated secretion of gonadotropins in primary rat pituitary cell cultures. This study demonstrates that inhibin and androgens: 1) differentially regulate gonadotropin levels, 2) cause changes in the amounts of bioactive and immunoactive FSH and LH released. In conclusion, differences in bio- and immuno-FSH and LH secretion suggest that the endocrine milieu results in not only quantitative but qualitative changes in the secreted gonadotropin isoforms.
authors.affiliation: Department of Medicine, VA Medical Center, Seattle, Washington 98108.
authors.forename: K D
authors.initials: KD
authors.lastname: Dahl
authors.forename: C A
authors.initials: CA
authors.lastname: Campen
authors.forename: D M
authors.initials: DM
authors.lastname: McGuinness
authors.forename: W
authors.initials: W
authors.lastname: Vale
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001141:Aromatase / Q000032:analysis; D002478:Cells, Cultured; D013196:Dihydrotestosterone / Q000494:pharmacology; D004347:Drug Interactions; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006062:Gonadotropins / Q000276:immunology / Q000378:metabolism*; D006107:Granulosa Cells / Q000166:cytology / Q000201:enzymology; D007265:Inhibins / Q000494:pharmacology*; D007985:Leydig Cells / Q000166:cytology / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D010902:Pituitary Gland / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000494:pharmacology
chemical_list: D000728:Androgens; D006062:Gonadotropins; D013196:Dihydrotestosterone; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D001141:Aromatase