title: [Polymerase chain reaction: general methodology].
abstract: The polymerase chain reaction method (PCR) is extremely sensitive and needs a precise control of the different steps and analytes of the reaction. In this article, we described the principle of the method and the characteristics of the main enzyme, the Taq polymerase. The concentration range of the analytes is reviewed to optimize the amplification of the nucleic acid sequence.
authors.affiliation: Laboratoire d'immunogénétique moléculaire, LIGM, URA CNRS 1191, université Montpellier II, France.
authors.forename: P
authors.initials: P
authors.lastname: Chuchana
mesh_terms: D066298:In Vitro Techniques; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods* / Q000592:standards
vernacular_title: Réactions de polymérisation en chaîne: méthodologie générale.