title: Calibration of quantitative histochemical methods: estimation of glycogen content of muscle fibers using the PAS reaction.
abstract: A fairly simple method for calibrating microdensitometric histochemical assays is described. The method is based on paired biochemical and histochemical assays on single freeze-dried skeletal muscle fibers which differ widely in their properties. As an example, the method is applied to investigate the validity of the periodic acid-Schiff (PAS) reaction for the microdensitometric estimation of glycogen content. Some problems that may interfere with the calibration are discussed.
authors.affiliation: Laboratory for Physiology, Free University, Amsterdam, The Netherlands.
authors.forename: W J
authors.initials: WJ
authors.lastname: van der Laarse
authors.forename: P
authors.initials: P
authors.lastname: van Noort
authors.forename: P C
authors.initials: PC
authors.lastname: Diegenbach
mesh_terms: D000818:Animals; D003720:Densitometry; D005260:Female; D005612:Freeze Drying; D006003:Glycogen / Q000032:analysis*; D009132:Muscles / Q000033:anatomy & histology / Q000737:chemistry*; D010503:Periodic Acid-Schiff Reaction; D014982:Xenopus laevis
chemical_list: D006003:Glycogen