Evaluation of three serological tests for the detection of human plague in northeast Brazil.
The passive haemagglutination (PHA) test, enzyme-linked immunosorbent assay (ELISA) and the dot enzyme-immunosorbent assay (DOT-ELISA) were used to detect the levels of IgG antibodies against the Fraction 1 (F1) antigen of Yersinia pestis in sera of plague-infected patients from Northeast Brazil. Twenty three selected PHA-positive sera of subjects with bacteriological confirmation of plague were also positive in the DOT-ELISA but only 19 were detected by the conventional ELISA technique. Another group of 186 serum samples from subjects diagnosed as plague-infected by clinical and epidemiological parameters, but PHA-negative, were screened with DOT-ELISA and 11 gave positive results. The specificity of the assays on the serological detection of plague was confirmed in inhibition tests using purified F1 antigen. These results suggest that DOT-ELISA can be an useful, simple and more sensitive alternative for the serodiagnosis of plague in Northeast Brazil.
de Almeida
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AM
Laboratorio de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brasil.
Ferreira
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LC
D000907:Antibodies, Bacterial / Q000032:analysis*; D001938:Brazil; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis*; D010930:Plague / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D015010:Yersinia pestis / Q000276:immunology
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G