Protective efficacy of different cell-wall fractions of Mycobacterium tuberculosis.
Immunization with various cell-wall fractions of M. tuberculosis H37Ra, progressively depleted of lipids (cell-wall-insoluble fraction; CWIF), soluble proteins (cell-wall core; CWC), mycolic acids and arabinogalactans (cell-wall-protein-peptidoglycan complex; CW-PPC) elicited significant levels of both humoral and cell-mediated immune response. Mice immunized with these fractions, when challenged with an LD50 dose of M. tuberculosis H37Rv, exhibited significant protection as revealed by high survival rates and decreased bacterial load in lungs, liver and spleen, as compared to nonimmunized animals.
Chugh
I B
IB
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Kansal
R
R
Vinayak
V K
VK
Khuller
G K
GK
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000302:isolation & purification; D001500:BCG Vaccine / Q000008:administration & dosage / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D002473:Cell Wall / Q000737:chemistry / Q000276:immunology*; D015169:Colony Count, Microbial; D005260:Female; D007111:Immunity, Cellular; D007114:Immunization; D008297:Male; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000302:isolation & purification; D010457:Peptidoglycan / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D014376:Tuberculosis / Q000517:prevention & control
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D001426:Bacterial Proteins; D010457:Peptidoglycan