OligoDNA-oligoamine hybrid for facile and sequence selective hydrolysis of RNA.
Oligoamines as catalytic sites for RNA hydrolysis are attached to 5'-end of [dT]16 using urethane bond. The [dT]16-pentaethylenehexamine hybrid promptly hydrolyzes poly[A] at pH 8, 50 degrees C. Definite importance of the complexation between the poly[A] and the [dT]16 moiety is confirmed by a marginal hydrolysis of poly[C]. Hydrolytic catalysis is ascribed to intramolecular acid-base cooperation of two or more amino residues in the oligoamines.
Yoshinari
K
K
Institute of Materials Science, University of Tsukuba, Ibaraki, Japan.
Inokawa
T
T
Komiyama
M
M
D002384:Catalysis; D006868:Hydrolysis; D011073:Polyamines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012260:Ribonucleases / Q000138:chemical synthesis; D013936:Thymidine / Q000737:chemistry*
D011073:Polyamines; D012313:RNA; C031122:pentaethylenehexamine; D012260:Ribonucleases; D013936:Thymidine