Lack of in vivo transcription of Acetabularia mediterranea 1175 bp ctDNA fragment homologous to the Drosophila per locus.
The period (per) locus of Drosophila melanogaster has a fundamental role in the expression of biological rhythms. A DNA sequence homologous to a short region of the Drosophila per locus was detected in the chloroplast of Acetabularia mediterranea. A 1175 bp DNA fragment containing the sequence was used as a probe in 'Northern' hybridization experiments. It was found that this DNA was not transcribed or only marginally transcribed in A. mediterranea, at least at the developmental stage just prior to cap formation. It seems that the 1175 bp ctDNA fragment is not involved in the Acetabularia biological rhythm mechanism.
Yang
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XP
Max-Planck-Institut für Zellbiologie, Abteilung Schweiger, Ladenburg, Germany.
Mittelsten Scheid
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O
D000076:Acetabularia / Q000235:genetics*; D000818:Animals; D001683:Biological Clocks / Q000235:genetics; D015152:Blotting, Northern; D002736:Chloroplasts / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004330:Drosophila / Q000235:genetics*; D014158:Transcription, Genetic
D004247:DNA