Different fatty-acid profiles in phosphoinositides from human fibroblastic meningiomas with or without chromosome 22 monosomy.
The fatty-acid composition of phosphoinositides derived from 13 human fibroblastic meningiomas, divided according to the presence or the absence of monosomy of chromosome 22, was analyzed. Phosphoinositides were separated into their 3 main components: phosphatidylinositol, phosphatidylinositol-4-phosphate, and phosphatidylinositol-4,5-bisphosphate. In all the tumors, regardless of cytogenetic differences, remarkable differences were detected between phosphatidylinositol and phosphatidylinositol-4-phosphate or phosphatidylinositol-4,5-bisphosphate. In meningiomas with 22 monosomy, there was a significant decrease in the relative amount of arachidonate in all the phosphoinositide fractions; this situation might determine diminished down-regulation of the corresponding diacylglycerol, derived by the phospholipase-C-stimulated hydrolysis of phosphoinositides, and, consequently, different protein-kinase-C stimulation.
Bordoni
A
A
Department of Biochemistry, University of Bologna, Italy.
Hrelia
S
S
Biagi
P L
PL
Berra
B
B
D002869:Chromosome Aberrations / Q000378:metabolism*; D025063:Chromosome Disorders; D002892:Chromosomes, Human, Pair 22; D005227:Fatty Acids / Q000737:chemistry*; D005347:Fibroblasts / Q000737:chemistry; D006801:Humans; D008579:Meningioma / Q000737:chemistry* / Q000235:genetics; D009006:Monosomy; D010716:Phosphatidylinositols / Q000737:chemistry*
D005227:Fatty Acids; D010716:Phosphatidylinositols