title: Regulation of a beta-galactoside-binding lectin and potential ligands during postnatal maturation of rat brain.
abstract: Variations in expression and activity of a beta-galactoside-specific lectin were quantified during postnatal maturation of the rat brain. Lectin expression, estimated from enzymatic immunoassay data, and lectin activity, evaluated by rabbit erythrocyte agglutination, are higher in the brains of young animals (5 days after birth) than in older ones (2 months of postnatal life). Concurrently, modifications in glycosylation during cellular differentiation were detected by affinoblotting using a biotinylated derivative of a beta-galactoside-specific brain lectin. This study shows that membrane and cytosolic glycoconjugates of the rat brain bear appropriate beta-galactoside moieties which may be recognized by an endogenous lectin expressed in the tissue itself in relation to brain maturation. The observed variations in the expression of lectin and complementary glycoconjugates can be correlated with significant events of rat brain development.
authors.affiliation: Laboratoire de Neurochimie, U.134, Hôpital de la Pitié-Salpêtrière, Paris, France.
authors.forename: W X
authors.initials: WX
authors.lastname: Li
authors.forename: R
authors.initials: R
authors.lastname: Joubert-Caron
authors.forename: H
authors.initials: H
authors.lastname: el Oumami
authors.forename: D
authors.initials: D
authors.lastname: Bladier
authors.forename: M
authors.initials: M
authors.lastname: Caron
authors.forename: N
authors.initials: N
authors.lastname: Baumann
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development*; D001923:Brain Chemistry / Q000502:physiology*; D002846:Chromatography, Affinity; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D037161:Galectins; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008024:Ligands; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
chemical_list: D037161:Galectins; D006388:Hemagglutinins; D007202:Indicators and Reagents; D008024:Ligands