[High precision, reproducibility and practical application of modified alanine aminotransferase determination with the microtiter plate].
In order to improve the alanine aminotransferase (ALT) assay by using microtiter plates, a new 96-channel multiple syringe of high precision and incubation chambers with precise regulation of the temperature operated by computer were developed. In accordance with the recommendation of the German Society of Clinical Chemistry for the optimized standard method, the ALT assay was modified for the procedure at 25 degrees C. The modification was comparable with the best conventional methods which, however, are very time-consuming. The ALT assay has the advantages of the microtiter plate, e.g., secure sample identification, on-line documentation and reduction of costs and time for the analysis.
Neppert
J
J
Institut für Transfusionsmedizin im Klinikum, Christian-Albrechts-Universität, Kiel, BRD.
Spindler
J
J
D000410:Alanine Transaminase / Q000097:blood*; D001771:Blood Banks; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D008111:Liver Function Tests / Q000295:instrumentation*; D012016:Reference Values; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*
D000410:Alanine Transaminase
Hohe Präzision, Reproduzierbarkeit und Praktikabilität der modifizierten Alanin-Amino-Transferase-Bestimmung auf der Mikrotiterplatte.