[Comparative sensitivity of different solid-phase immunoassays for detecting HBsAg and anti-HCV in blood].
The sensitivities of seven methods of enzyme-immunoassay for HBsAg detection, as well as of twelve immunoassays for detecting antibody to HCV, were compared, in an attempt to evaluate the need for an official technical validation of such methods prior to its commercialization in Spain. Significant differences were seen for the sensitivities of these methods, which may influence the proficiency of blood bank screening for HBsAg and anti-HCV for preventing cases of post-transfusional viral hepatitis. As a conclusion, it is recommended to establish legal regulations for pre-marketing validation of such assays in Spain, as well as to take the results obtained in these evaluation studies as the basis for selecting those tests which may be more convenient for screening purposes at the blood banks.
León
P
P
Laboratorio de Referencia de Hepatitis, Centro Nacional de Microbiología, Virología e Inmunología Sanitarias, Majadahonda, Madrid.
López
J A
JA
Domingo
C J
CJ
Echevarría
J M
JM
D001771:Blood Banks / Q000592:standards; D001782:Blood Donors; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D006509:Hepatitis B / Q000175:diagnosis / Q000517:prevention & control* / Q000635:transmission; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006526:Hepatitis C / Q000175:diagnosis / Q000517:prevention & control* / Q000635:transmission; D018937:Hepatitis C Antibodies; D006801:Humans; D007124:Immunoenzyme Techniques; D008403:Mass Screening / Q000379:methods*; D011863:Radioimmunoassay; D012680:Sensitivity and Specificity; D065227:Transfusion Reaction
D006508:Hepatitis Antibodies; D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies
Sensibilidad comparativa de distintos inmunoensayos en fase sólida para detección de HBsAg y anti-VHC en suero.