title: Characterization of a lipopolysaccharide encoded by a recombinant Shigella sonnei plasmid in Escherichia coli K-12.
abstract: The genetic information to synthesize the S-specific region of Shigella sonnei phase I lipopolysaccharide (LPS) is localized on a 180 kb plasmid which is lost quite readily. A recombinant plasmid derivative remaining stable in the bacteria was shown to determine the S-specific region of the LPS which is completely identical with that of a S. sonnei phase I strain following transfer in Escherichia coli K-12. However, the length control in polysaccharide biosynthesis is lost at least partially.
authors.affiliation: Robert-Koch-Institut des Bundesgesundheitsamts, Wernigerode, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Seltmann
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: H
authors.initials: H
authors.lastname: Tschäpe
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens; D010957:Plasmids; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D011995:Recombination, Genetic; D012764:Shigella sonnei / Q000235:genetics* / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial