Principal and intercalated cells in primary cultures of rabbit renal collecting tubules revealed by monoclonal antibodies.
In this study we describe the production and characterization of two monoclonal antibodies (mAb 503 and mAb 703) raised against the apical membrane of rabbit cortical collecting tubule (CCT) cells. The specificity of the two monoclonal antibodies was studied by immunoelectron microscopy on kidney sections. These antibodies were used to identify principal and intercalated cells in primary cultures of CCT. To assess the maintenance of the basic characteristics of the cortical collecting cells during the growth process we determined the biochemical and electrophysiological properties of cultured CCT. Of the monoclonal antibodies produced mAb 503 was specifically directed against the luminal membrane of intercalated cells as shown by immunoelectron microscopy. mAb 703 bound specifically the apical membrane of the principal cells. In primary cultures of CCT mAb 503 and mAb 703 bound antigens present on the apical membrane of different cells and permitted the study of the distribution of the two cell types. Results showed the maintenance of the epithelial polarity of cultured CCT and the expression of specific antigens.
Tauc
M
M
Département de Biologie cellulaire et moléculaire, CEN Saclay, Gif-sur-Yvette, France.
Koechlin
N
N
Jamous
M
M
Ouaghi
E M
EM
Gastineau
M
M
Le Moal
C
C
Poujeol
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001666:Binding Sites, Antibody / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D007672:Kidney Cortex / Q000166:cytology*; D007685:Kidney Tubules, Collecting / Q000166:cytology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D011817:Rabbits
D000911:Antibodies, Monoclonal