title: [The 3-dimensional organization of the nucleolus and the nucleolus organizer regions in differentiated cells. III. Changes in the nature of the distribution of argentophilic proteins in the nucleoli of mouse hepatocytes with stimulation of the hepatocytes to proliferation].
abstract: The stereological and morphological analysis of Ag-positive zones revealed the principal difference in quantity and distribution of Ag-NOR proteins in reticulated and nucleolonemal nucleoli of mouse hepatocytes. It is shown that reticulated nucleoli of resting hepatocytes are characterized with the presence of discrete rounded Ag-positive zones, whose quantitative parameters correspond to the fibrillar centers and dense fibrillar component taken together. The analysis of the three-dimensional structure of Ag-positive zones of nucleolonemal nucleoli has revealed considerable changes in quantity and distribution of Ag-NOR proteins due to proliferative stimuli. 22 hours following operation, a continuous, strongly winding strand of nucleolonema is revealed in the nuclei. Taking into account that Ag-NOR proteins are associated with the transcriptionally active regions of rDNA it may be suggested that this structural conversion was due to decondensation and activation of inactive regions of r-chromatin. A considerable growth of Ag-positive zone volume during the conversion of reticulated nucleoli into the nucleolonemal type may be explained by the increase in Ag-NOR protein contents.
authors.forename: P V
authors.initials: PV
authors.lastname: Chelidze
authors.forename: T V
authors.initials: TV
authors.lastname: Mdivani
authors.forename: D V
authors.initials: DV
authors.lastname: Dzidziguri
authors.forename: E O
authors.initials: EO
authors.lastname: Cherkeziia
authors.forename: T G
authors.initials: TG
authors.lastname: Tumanishvili
authors.forename: G D
authors.initials: GD
authors.lastname: Tumanishvili
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002466:Cell Nucleolus / Q000648:ultrastructure*; D006498:Hepatectomy; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D008115:Liver Regeneration / Q000502:physiology; D051379:Mice; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000378:metabolism*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D016622:Silver Staining; D013997:Time Factors
chemical_list: D009687:Nuclear Proteins
vernacular_title: Trekhmernaia organizatsiia iadryshka i iadryshko-obrazuiushchikh raĭonov differentsirovannykh kletok. III. Izmenenie kharaktera raspredeleniia argentofil'nykh belkov iadryshek gepatotsitov myshi pri stimuliatsii gepatotsitov k proliferatsii.