title: A sensitive enzymatic method (SK-013) for detection and quantification of specific periodontopathogens.
abstract: Porphyromonas gingivalis, Bacteroides forsythus, and Treponema denticola have been found to predominate in periodontal pockets of patients with adult periodontitis. These microorganisms hydrolyze the synthetic peptide N-benzoyl-DL-arginine-2-naphthylamide (BANA). In this study, we developed an enzymatic method, designated SK-013, to detect the existence of these microorganisms in subgingival plaque bacteria. This enzymatic method was based on the observation of the hydrolysis of N-carbobenzoxy-glycyl-glycyl-arginyl-3,5-dibromo-4-hydroxyaniline (N-CBz-Gly-Gly-Arg-DBHA) and made more sensitive by adding an enhancing system. The SK-013 was specifically positive for P. gingivalis, B. forsythus, T. denticola, and some strains of Capnocytophaga species, but was not specific for any of the other bacterial strains tested. This SK-013 system may be valuable for detection and quantification of periodontal disease-associated bacteria in subgingival plaque and thus for diagnosis of periodontal infections.
authors.affiliation: Department of Microbiology, Tokyo Dental College, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ishihara
authors.forename: Y
authors.initials: Y
authors.lastname: Naito
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: I
authors.initials: I
authors.lastname: Takazoe
authors.forename: K
authors.initials: K
authors.lastname: Okuda
authors.forename: T
authors.initials: T
authors.lastname: Eguchi
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
authors.forename: N
authors.initials: N
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Yamasaki
authors.forename: K
authors.initials: K
authors.lastname: Hasegawa
mesh_terms: D001439:Bacteroides / Q000201:enzymology; D001587:Benzoylarginine-2-Naphthylamide; D002206:Capnocytophaga / Q000201:enzymology; D004796:Clinical Enzyme Tests / Q000379:methods*; D015169:Colony Count, Microbial; D010450:Endopeptidases / Q000032:analysis*; D006801:Humans; D010514:Periodontal Pocket / Q000382:microbiology; D010518:Periodontitis / Q000175:diagnosis* / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000201:enzymology; D014208:Treponema / Q000201:enzymology
chemical_list: D001587:Benzoylarginine-2-Naphthylamide; D010450:Endopeptidases