[Production of a vaccine against enterotoxemia from Clostridium perfringens strains isolated in the field].
We have isolated eight strains of C. perfringens from cases of enterotoxaemia. Five of these strains have revealed themselves toxic with respective types (type "A":2, type "C":2, type "D":1). In order to produce anti-enterotoxaemia vaccine, we have proceeded at the cultivation in fermenter of isolated strains and reference strains CWA 35, CWC and CWD AF. At the end of fermentation, we have evaluated the two following parameters: obtained biomass, and toxin titers. With the two classes of strains we reached an important biomass but toxins titers relatively weak comparatively to that which is usually required. It will be necessary then, to demonstrate the immunogen value of the produced vaccines by testing their efficacity.
Cherfaoui
S
S
Kadra
B
B
D000693:Anaerobiosis; D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000276:immunology; D001431:Bacteriological Techniques; D002135:Calcium-Binding Proteins; D003016:Clostridium perfringens / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D003470:Culture Media; D004767:Enterotoxemia / Q000382:microbiology / Q000517:prevention & control*; D051379:Mice; D012756:Sheep / Q000382:microbiology; D012757:Sheep Diseases / Q000382:microbiology*; D010738:Type C Phospholipases
D001427:Bacterial Toxins; D001428:Bacterial Vaccines; C031884:CPB protein, Clostridium perfringens; D002135:Calcium-Binding Proteins; C029352:Clostridium perfringens delta-toxin; D003470:Culture Media; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens
Contribution à la production d'un vaccin anti-entérotoxémie à partir de souches de Clostridium perfringens isolées sur le terrain.