[Synthesis and supramolecular organization of components of the extracellular matrix by fibroblasts cultured in a collagen lattice].
Fibroblasts cultured in a collagen gel contract and organize the gel into a three-dimensional matrix of collagen fibers. Within this matrix, the fibroblast cell cycle is blocked at the G1 phase but also at the G2 phase. The fibroblasts produce the main extracellular matrix components (collagen, noncollagen proteins, glycosaminoglycans), although in small amounts. Studies using this in vitro model with radiolabeled precursor substances (14C proline, 3H glucosamine) demonstrated production of supermolecular complexes which resisted to proteolysis by pepsin and collagenase and could not be isolated by saline precipitation. Polyclonal antibodies identified type I collagen, type VI collagen and fibronectin in this coherent supermolecular structure. The presence of glycosaminoglycans was also demonstrated by alcian blue precipitation.
Frey
J
J
Laboratoire de Biochimie, Faculté de Médecine, Saint-Etienne, France.
Rattner
A
A
Chamson
A
A
D002478:Cells, Cultured; D002848:Chromatography, DEAE-Cellulose; D003094:Collagen / Q000096:biosynthesis*; D003470:Culture Media; D005109:Extracellular Matrix / Q000737:chemistry / Q000502:physiology*; D005347:Fibroblasts / Q000737:chemistry / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D011509:Proteoglycans / Q000096:biosynthesis*
D003470:Culture Media; D011509:Proteoglycans; D003094:Collagen
Synthèse et organisation supramoléculaire de composants de la matrice extracellulaire par des fibroblastes cultivés dans une trame de collagène.