title: Amyloid precursor protein mRNA encoding the Kunitz protease inhibitor domain is increased by kainic acid-induced seizures in rat hippocampus.
abstract: A 168-nucleotide exon, found in alternatively spliced amyloid precursor protein (APP) mRNAs, encodes a Kunitz protease inhibitor (KPI) domain. Kainic acid (ip) caused a selective increase of KPI mRNA in rat hippocampus. By in situ hybridization, KPI mRNA was induced in the neuronal layers of the hippocampus 11-12 h after the onset of kainate-induced seizures. The kainate-induced elevation of the KPI-containing APP-770 mRNA was blocked by pretreatment with the anticonvulsant pentobarbital. These data suggest that kainate-induced seizures cause alterations in APP RNA stability and/or processing in rat hippocampal neurons.
authors.affiliation: Ethel Percy Andrus Gerontology Center, University of Southern California, Los Angeles 90089-0191.
authors.forename: D A
authors.initials: DA
authors.lastname: Willoughby
authors.forename: S A
authors.initials: SA
authors.lastname: Johnson
authors.forename: G M
authors.initials: GM
authors.lastname: Pasinetti
authors.forename: G
authors.initials: G
authors.lastname: Tocco
authors.forename: I
authors.initials: I
authors.lastname: Najm
authors.forename: M
authors.initials: M
authors.lastname: Baudry
authors.forename: C E
authors.initials: CE
authors.lastname: Finch
mesh_terms: D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000378:metabolism*; D017403:In Situ Hybridization; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D010424:Pentobarbital / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000235:genetics*; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000235:genetics*
chemical_list: D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger; D014360:Trypsin Inhibitor, Kunitz Soybean; D010424:Pentobarbital; D007608:Kainic Acid