[Effect of interleukins and somatomedins on the production of neurotensin by cell line SH-SY5Y derived from human neuroblastoma].
The goal of this study was to investigate the regulation by insulin-like growth factors 1 and 2, and interleukins on the production of neurotensin in the SH-SY5Y cell line derived from a human neuroblastoma. Cultures were performed in RPMI1640 culture medium with heated foetal calf serum 12%. After 24 hrs. of fasting without serum, interleukins-1 alpha, IL-2, IL-4 and insulin-like growth factors 1 and 2 were added. Results showed: 1) A mitogenic effect of ILs (p < 0.001) and of IGFs (p < 0.001). 2) The presence of neurotensin in HCl0.1N cellular extracts (0.06 fmol/micrograms protein). 3) The increase of cellular neurotensin content in the presence of IL-4 (560%), IL-2 (480%), IGF-1 (610%) and IGF-2 (200%). Our results indicate that the human neuroblastoma cell line SH-SY5Y produces neurotensin and that ILs and IGFs act in vitro to modulate this production.
Loret
C
C
I.N.S.E.R.M. U.142, Hôpital Saint-Antoine, Paris.
Leneuve
P
P
Bozzola
M
M
Lhiaubet
A M
AM
Schimpff
R M
RM
Rostene
W
W
D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007378:Interleukins / Q000494:pharmacology*; D009447:Neuroblastoma / Q000473:pathology*; D009496:Neurotensin / Q000096:biosynthesis* / Q000187:drug effects; D013002:Somatomedins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D007378:Interleukins; D013002:Somatomedins; D009496:Neurotensin
Effet des interleukines et des somatomédines sur la production de neurotensine par la lignée cellulaire SH-SY5Y dérivée d'un neuroblastome humain.