[Simple spike response of cerebellar Purkinje cells to stimulation of C-fiber in saphenous nerve].
Simple spike of cerebellar Purkinje cells (PC-SS) was recorded with microelectrode. In the NCCVF (normalized cross-covariance function) histogram, spontaneous PC-SS does not show obvious peak. When the saphenous nerve is stimulated at lower intensities, which elicits the A-fiber input only, the discharge response (A-CED) consists of an early component with a latency of 16.7 +/- 0.9 ms and a late component with a latency of 270.8 +/- 12.8 ms. After A-fibers are blocked selectively by polarizing current, the stimulation at a suprathreshold strength for C-fiber evokes a characteristic response (C-CED) with a latency of 142.4 +/- 4.3 ms. However, the C-CED can not be evoked by the inputs of A- and C-fiber simultaneously. In NPSDF histogram, the spontaneous activities of PC-SS can be divided into two groups, the high and the low peak group. The high peak group (n = 15) has a peak energy value of 15.7 +/- 4.7 x 10(-3) and peak frequency of 4.07 +/- 1.69 Hz. A-fiber input causes an increase of the peak value, while C-fiber input causes a decrease. The low peak group (n = 16) has a peak energy value 8.4 +/- 1.4 x 10(-3) and peak frequency of 3.67 +/- 2.90 Hz. Both A-fiber and C-fiber inputs cause an increase of the peak value, but the effect of A-fiber input was more prominent. The results show that the pure C-fiber input can reach the cerebellar PC and elicit characteristic simple spike response.
Wu
J
J
Department of Physiology, Sun Yat-Sen University of Medical Science, Guangzhou.
Chen
P X
PX
D000818:Animals; D002415:Cats; D002531:Cerebellum / Q000166:cytology / Q000502:physiology*; D004558:Electric Stimulation; D005071:Evoked Potentials; D007866:Leg / Q000294:innervation; D009412:Nerve Fibers / Q000502:physiology*; D011689:Purkinje Cells / Q000502:physiology*; D011930:Reaction Time / Q000502:physiology