[Allergen-stimulated release of prostaglandin E, F2 alpha by alveolar macrophages from patients with allergic asthma].
Bronchoalveolar lavage (BAL) was performed in healthy subjects (n = 12) and patients with bronchial asthma (n = 11). Between the two groups there was no significant difference in the total number of cells and the percentage of alveolar macrophages (AM), lymphocytes (L), neutrophils (N) and eosinophils (E) in BAL fluids. When AM were cultured in vitro and stimulated with dermatophagoides farinae (DPF) antigen, the amount of PGE released by AM was significantly increased in the asthmatics. When the asthmatics, AM were sensitized with specific IgE positive serum and stimulated with DPF, they released more PGE and PGF2 alpha than those when specific IgE negative serum was used (P < 0.01). The PGE/PGF2 alpha ratio was significantly decreased. There was positive correlation between the decreased value of PGE/PGF2 alpha and patients, MCH-PC20. The increase in the amount of PGE and PGF2 alpha released and the decrease of PGE/PGF2 alpha ratio might play an important role in the pathogenesis of allergic asthma.
Ni
D T
DT
Department of Respiratory Diseases, Xijing Hospital, Fourth Military Medical University, Xian.
Sun
B
B
D000818:Animals; D000941:Antigens; D001249:Asthma / Q000209:etiology / Q000276:immunology / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D002478:Cells, Cultured; D015237:Dinoprost / Q000378:metabolism*; D006801:Humans; D007114:Immunization; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008925:Mites / Q000276:immunology; D011458:Prostaglandins E / Q000378:metabolism*
D000941:Antigens; D011458:Prostaglandins E; D015237:Dinoprost