Biochemical characterization of binding sites for dihydropyridine and omega-conotoxin in brain of adult chicken.
Binding studies using the calcium channel blockers omega-conotoxin and dihydropyridine revealed a rather equal amount of binding sites in brain from adult chicken. The omega-conotoxin binding sites could be solubilized using digitonin, without substantial loss, whereas a great decrease in dihydropyridine binding sites was observed, indicating that both types of binding sites have different sensitivity to solubilization. In contrast to ion exchange chromatography where both binding sites comigrated, glycoprotein affinity chromatography led to a different partition of the binding sites in the flow through and eluate fractions. Our results indicate that both types of calcium channel blockers bind to different targets in adult chicken.
Zgaga-Griesz
A
A
University of Freiburg, Institute of Molecular Cell Biology, FRG.
Woscholski
R
R
Straub
P
P
Hug
H
H
Marmé
D
D
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000378:metabolism*; D015220:Calcium Channels; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004095:Dihydropyridines / Q000378:metabolism; D017275:Isradipine; D007700:Kinetics; D010456:Peptides, Cyclic / Q000378:metabolism; D017981:Receptors, Neurotransmitter / Q000302:isolation & purification / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000302:isolation & purification / Q000378:metabolism*; D012995:Solubility; D020960:omega-Conotoxins
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D004095:Dihydropyridines; D010456:Peptides, Cyclic; D017981:Receptors, Neurotransmitter; D011978:Receptors, Nicotinic; D020960:omega-Conotoxins; C053157:omega-conotoxin receptor; C053423:Conus magus toxin; D017275:Isradipine