Siderophore production and membrane alterations by Bordetella pertussis in response to iron starvation.
Bordetella pertussis was grown in iron (Fe)-free defined medium to limit the growth of the organism. Doubling times of the Fe-starved organism increased by approximately 1 h, and a 40% reduction in the final extent of growth in Fe-depleted medium was observed. Under these conditions, a hydroxamate siderophore named bordetellin was secreted by B. pertussis. Lactoferrin and transferrin supported growth of B. pertussis even when the protein was sequestered inside dialysis tubing. This suggested that binding of lactoferrin and transferrin to B. pertussis was not essential and that bordetellin production plays a major role in Fe uptake. Solid-phase dot blot assays indicated weak binding of lactoferrin to the cell surface, consistent with previous reports of a lactoferrin receptor. Three new proteins of 97, 77, and 63 kDa were synthesized in response to Fe starvation. Fe-inducible proteins of 103, 72, 24, 21, and 18 kDa were also observed. The synthesis of lipopolysaccharide was also altered by Fe availability.
Agiato
L A
LA
Department of Microbiology, State University of New York, Buffalo 14214.
Dyer
D W
DW
D015153:Blotting, Western; D001886:Bordetella pertussis / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005296:Ferrous Compounds / Q000494:pharmacology; D006427:Hemin / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000378:metabolism*; D000090463:Iron Deficiencies; D007781:Lactoferrin / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D008954:Models, Biological; D011956:Receptors, Cell Surface / Q000032:analysis; D011990:Receptors, Transferrin / Q000032:analysis; D017262:Siderophores; D014168:Transferrin / Q000494:pharmacology
D005296:Ferrous Compounds; D007502:Iron Chelating Agents; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D017262:Siderophores; D014168:Transferrin; C038340:lactoferrin receptors; C020748:ferrous sulfate; D006427:Hemin; D007501:Iron; D007781:Lactoferrin