title: [Extracellular ribonuclease from Bacillus pumilus].
abstract: The extracellular ribonuclease (RNAse Bp) was isolated from the cultural medium filtrate of Bacillus pumilus by ammonium sulfate precipitation and two stages of ion-exchange chromatography on carboxymethyl- and phospho-cellulose columns. The amino acid composition and N-terminal amino acid residue have been determined. The kinetic parameters of cleavage reaction of synthetic polynucleotides have been measured. According to their structural homology RNAse Bp has been shown to be similar to RNAses Ba and Bi. Catalytic properties of the enzyme are very close to RNAse Bi.
authors.forename: N K
authors.initials: NK
authors.lastname: Struminskaia
authors.forename: V L
authors.initials: VL
authors.lastname: Ivaĭlovskiĭ
authors.forename: A A
authors.initials: AA
authors.lastname: Dement'ev
authors.forename: G P
authors.initials: GP
authors.lastname: Moiseev
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Fedosov
authors.forename: G I
authors.initials: GI
authors.lastname: Iakovlev
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001407:Bacillus / Q000201:enzymology*; D002384:Catalysis; D004795:Enzyme Stability; D005095:Exoribonucleases / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D005095:Exoribonucleases
vernacular_title: Vnekletochnaia ribonukleaza iz Bacillus pumilus.