title: Immunoblots with rhodopsin antisera suggest that a purified mu opioid binding protein has structural characteristics of a G-protein-coupled receptor.
abstract: A mu opioid binding protein (OBP), previously purified to homogeneity from bovine striatal membranes, was examined by immunoblotting with six antisera against bovine rhodopsin. An antibody against the carboxyl-terminal tail of rhodopsin and one against membrane-associated rhodopsin gave strong signals at the appropriate molecular mass (65 kDa). An antibody directed against the first cytoplasmic loop of rhodopsin was weakly reactive. Three other antibodies did not recognize OBP. This pattern of crossreactivity was identical to that previously seen with beta-adrenergic receptors. The existence of domains in the OBP, which are antigenically similar to those in two other guanine nucleotide regulatory protein-coupled receptors, supports the hypothesis that mu opioid receptors have the structure characteristic of this receptor family.
authors.affiliation: Department of Psychiatry, New York University Medical Center, NY 10016.
authors.forename: T L
authors.initials: TL
authors.lastname: Gioannini
authors.forename: E R
authors.initials: ER
authors.lastname: Weiss
authors.forename: G L
authors.initials: GL
authors.lastname: Johnson
authors.forename: J M
authors.initials: JM
authors.lastname: Hiller
authors.forename: E J
authors.initials: EJ
authors.lastname: Simon
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry; D003342:Corpus Striatum / Q000737:chemistry; D003429:Cross Reactions; D019204:GTP-Binding Proteins / Q000502:physiology*; D011957:Receptors, Opioid / Q000737:chemistry / Q000276:immunology*; D017450:Receptors, Opioid, mu; D012243:Rhodopsin / Q000276:immunology
chemical_list: D011957:Receptors, Opioid; D017450:Receptors, Opioid, mu; D012243:Rhodopsin; D019204:GTP-Binding Proteins