Antibodies to HPV-16 E6 and E7 proteins as markers for HPV-16-associated invasive cervical cancer.
Transforming proteins E6 and E7 of human papillomaviruses (HPVs) are consistently expressed in HPV-associated cervical cancers. In ELISA with four HPV-16 E6-E7 peptides, patients with HPV-16-associated invasive cervical cancer (group 1) had a greater seroreactivity than all other groups, which included patients with HPV-16-associated cervical intraepithelial neoplasia, invasive cervical cancer patients without HPVs, and unaffected controls. A larger proportion of group 1 sera, as compared to sera of all other groups, was reactive with at least one peptide (49% vs 17-27%), and with two or more peptides (22% vs 0-6%). A clear difference between group 1 and all other groups was also found for high ELISA absorbance values to at least one peptide (22% vs 0-8%). This high seroreactivity of group 1 sera was confirmed by a radioimmunoprecipitation assay with in vitro transcribed and translated HPV-16 E7 protein. Sera from 50% of group 1 but only 3% of controls were reactive in this test. Antibodies to HPV-16 E6 and E7 proteins appear to be virus-specific and disease state-specific markers of HPV-associated cervical cancer.
Müller
M
M
Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Viscidi
R P
RP
Sun
Y
Y
Guerrero
E
E
Hill
P M
PM
Shah
F
F
Bosch
F X
FX
Muñoz
N
N
Gissmann
L
L
Shah
K V
KV
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000276:immunology*; D014408:Biomarkers, Tumor; D016022:Case-Control Studies; D003105:Colombia; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D027383:Papillomaviridae / Q000276:immunology*; D050725:Papillomavirus E7 Proteins; D010455:Peptides / Q000276:immunology; D012097:Repressor Proteins; D013030:Spain; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000276:immunology*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology
D000914:Antibodies, Viral; D014408:Biomarkers, Tumor; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D010455:Peptides; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16