[Diaphanization: modifications of organ weight and volume].
The diaphanization of organs previously injected with coloured substances is an excellent auxiliary method of numerous morphological studies. The authors studied 52 organs, among 15 were taken from human beings and 37 from dogs, with the aim of knowing theirs weight and volume modifications after diaphanization. The organs were diaphanizated with several impregnations in 10% formaldehyde, perydrol, acetone and at least embedding in a mixture of methylsalicylate and benzyl benzoate by vacuum process. After each embedding, weight and volume of each organ were measured. The variations that the organs suffered were statistically considered, under a few aspects: 1-global organs' variations after each embedding 2-different organs' variations 3-comparison between human being and experimental animals. In all cases, the authors noticed a great decrease of weight and volume, especially after dehydration steps with acetone and clearing with the mixture of methylsalicylate and benzyl benzoate. We also didn't find any significant difference between human organs and those taken from dogs.
Bernardes
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A
Laboratoire d'Anatomie, Faculté de Médecine, COIMBRA, Portugal.
Esperança-Pina
J A
JA
Goyri O'Neill
J E
JE
da Veiga
A
A
D000818:Animals; D016477:Artifacts; D004285:Dogs; D006801:Humans; D009929:Organ Size / Q000502:physiology*; D014172:Transillumination
La diaphanisation: modifications du poids et du volume des organes.