title: A multi-allergen ELISA screening method. Comparison with Pharmacia CAP system and Phazet skin prick test.
abstract: Eighty consecutive subjects, coming to our service as suffering from conjunctivitis and/or rhinitis and/or asthma, were investigated by a new multi-allergen enzymatic screening immunoassay (Profilo, Bio-Allergy, Rome, Italy) regarding to serum specific IgE for groups of inhalant allergens (Dermatophagoides, Parietaria, grasses, trees, animal danders). These groups include allergens which are most frequently responsible of allergy in our country. Indices of diagnostic accuracy of this assay were analyzed in relation to skin prick test (SPT, Phazet, Pharmacia), as in vivo reference test, and Pharmacia CAP System, as in vitro reference test, comparing the results achieved by these tests in the same group of subjects. Profilo showed high significant (p < 0.0005) correlations and satisfactory indices of diagnostic accuracy (sensitivity, specificity, predictive values of positive and negative, and efficiency) for the tested allergens with reference to both Phazet SPT and CAP System. In addition, the method appeared very rapid and simple, so that it could be performed in the physician's office.
authors.affiliation: Allergy and Clinical Immunology Service, DIMI, University of Genoa, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Scordamaglia
authors.forename: G
authors.initials: G
authors.lastname: Passalacqua
authors.forename: P
authors.initials: P
authors.lastname: Pisati
authors.forename: G
authors.initials: G
authors.lastname: Moscato
authors.forename: R
authors.initials: R
authors.lastname: Bertoletti
authors.forename: S
authors.initials: S
authors.lastname: Ruffoni
authors.forename: G W
authors.initials: GW
authors.lastname: Canonica
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D001249:Asthma / Q000276:immunology*; D003233:Conjunctivitis, Allergic / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D007428:Intradermal Tests; D008875:Middle Aged; D011237:Predictive Value of Tests; D011863:Radioimmunoassay; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D012680:Sensitivity and Specificity; D016037:Single-Blind Method
chemical_list: D000485:Allergens; D007073:Immunoglobulin E