title: [Cloning the gene for vaccinia virus strain L-IVP growth factor in Escherichia coli].
abstract: The growth factor gene of the vaccinia virus LIVP strain has been primarily cloned in a 4.3 kbp long BamHI-EcoRI fragment and then subcloned in a 440 bp fragment. It was shown that clone 4 of the LIVP strain contains a single copy of this gene while the WR strain contains a repeat. The gene is located on a 4.3 kbp BamHI-EcoRI fragment but not on a 2.2 kbp fragment and has four nucleotide changes, three of which result in amino acid substitutions.
authors.forename: V N
authors.initials: VN
authors.lastname: Pak
authors.forename: V V
authors.initials: VV
authors.lastname: Raspopin
authors.forename: V S
authors.initials: VS
authors.lastname: Petrov
authors.forename: R K
authors.initials: RK
authors.lastname: Kurmanov
authors.forename: N V
authors.initials: NV
authors.lastname: Mamaeva
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral; D004926:Escherichia coli; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C051087:vaccinia growth factor
vernacular_title: Klonirovanie v kletkakh Escherichia coli gena faktora rosta virusa ospovaktsiny shtamma L-IVP.