Measurement of nerve growth factor-like immunoreactivity in human brain using an anti-mouse-NGF enzyme immunoassay.
Nerve growth factor (NGF) like immunoreactivity, expressed as mouse 2.5S nerve growth factor equivalents, was evaluated in three structures of the human brain post-mortem using a commercially available enzyme immunoassay. Regional differences in NGF-like immunoreactivity were observed. Highest levels were found in hippocampus (148 pg/g) compared to putamen (76 pg/g) and frontal cortex (34 pg/g). In addition, these results suggest differences in the distribution of brain NGF between human and rodent, where relatively high levels of NGF are found in the cortex.
Bertrand
P
P
Rhône-Poulenc Rorer, Centre de Recherche de Vitry-Alfortville, Vitry Sur Seine, France.
Roger
O
O
Houlgatte
R
R
Javoy-Agid
F
F
Brandel
J P
JP
Doble
A
A
Blanchard
J C
JC
D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001923:Brain Chemistry; D005625:Frontal Lobe / Q000737:chemistry; D006624:Hippocampus / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D009414:Nerve Growth Factors / Q000032:analysis*; D009928:Organ Specificity; D011699:Putamen / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013045:Species Specificity
D009414:Nerve Growth Factors