Anti-GM3-lactam monoclonal antibodies of the IgG type recognize natural GM3-ganglioside lactone but not GM3-ganglioside.
Immunization of mice with a synthetic GM3-lactam-BSA (bovine serum albumin) conjugate (designed to emulate the corresponding natural GM3-lactone conjugate), followed by fusion of splenocytes with myeloma cells, gave rise to more than 300 monoclonal hybridomas producing antibodies to GM3-lactam-BSA, which did not react with Glc-BSA and BSA. Eight antibody clones were randomly chosen from the positive 300 hybridomas. The eight clones, all belonging to the IgG class, were unreactive against GM3-ganglioside, whereas two antibodies (P5-1 and P5-3, both IgG1, kappa) reacted with GM3-ganglioside lactone. Binding of these two antibodies to the GM3-lactam-BSA conjugate was inhibited by soluble glycosides of GM2-, GM3-, and GM4-lactam and by GM3- and GM4-lactam, respectively, but not by Gb3 or asialo-GM1 and GM2-saccharides. A third antibody (P3; IgG2b, kappa) was inhibited by GM2-, GM3-, and GM4-lactam, but did not recognize GM3-ganglioside lactone.
Ding
K
K
Department of Medical Cell Genetics, Karolinska Institutet, Stockholm, Sweden.
Rosén
A
A
Ray
A K
AK
Magnusson
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003429:Cross Reactions; D005679:G(M3) Ganglioside / Q000031:analogs & derivatives* / Q000276:immunology*; D006825:Hybridomas; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure
D000911:Antibodies, Monoclonal; D005679:G(M3) Ganglioside; D007074:Immunoglobulin G; C081657:ganglioside GM3 lactam; C047143:ganglioside GM3 lactone