title: Culture of human osteoblasts on demineralised human bone. Possible means of graft enhancement.
abstract: We cultured human osteoblasts from trabecular bone explants and confirmed their phenotype by alkaline phosphatase assay, increased cyclic adenosine monophosphate production in response to prostaglandin E2 and radiographic micro-analysis of nodules of calcification. The osteoblasts were seeded on to demineralised human bone fragments and examined at ten-day intervals over a 50-day period by scanning electron microscopy. During this time the bank bone became progressively repopulated by the cultured osteoblasts. This system may offer a means of graft enhancement in elective orthopaedic and maxillofacial surgery by delivery of cultured autologous human osteoblasts to bone defects.
authors.affiliation: School of Biomedical Science, Queen's University of Belfast, Northern Ireland.
authors.forename: P C
authors.initials: PC
authors.lastname: Nolan
authors.forename: R M
authors.initials: RM
authors.lastname: Nicholas
authors.forename: B J
authors.initials: BJ
authors.lastname: Mulholland
authors.forename: R A
authors.initials: RA
authors.lastname: Mollan
authors.forename: D J
authors.initials: DJ
authors.lastname: Wilson
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology*; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D003648:Decalcification Technique; D015232:Dinoprostone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006082:Graft Enhancement, Immunologic / Q000379:methods*; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010641:Phenotype
chemical_list: D000242:Cyclic AMP; D000469:Alkaline Phosphatase; D015232:Dinoprostone