An erythroid species-specific antigen of swine detected by a monoclonal antibody.
A monoclonal antibody (1AC11) has been produced which recognized the glycophorin of swine red blood cells. 1AC11 was specific for swine membrane erythrocytes. No other swine cells (leukocytes, macrophages, kidney and testis cells) nor red blood cells from all the tested mammalian species (goat, human, sheep, cattle, horse, rabbit, cat and guinea pig) were recognized. There was no blood group activity detected. Immunocytochemical analysis of blood vessel in the swine pituitary tissue showed that besides membrane erythrocytes, cytoplasmic molecules were recognized in some cells. Immunoblot analysis of both membrane and aqueous phase of chloroform/methanol fractions from swine erythrocytes showed that the monoclonal antibody 1AC11 reacts with the major sialoglycoprotein of apparent molecular weight 45,000 daltons.
Llanes
D
D
Departamento de Genética, Universidad de Córdoba, Spain.
Nogal
M L
ML
Prados
F
F
del Val
M
M
Viñuela
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004910:Erythrocyte Membrane / Q000276:immunology*; D006021:Glycophorins / Q000276:immunology* / Q000302:isolation & purification; D008322:Mammals / Q000097:blood / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008970:Molecular Weight; D009928:Organ Specificity; D010902:Pituitary Gland / Q000098:blood supply / Q000166:cytology / Q000276:immunology; D013045:Species Specificity; D013552:Swine / Q000097:blood / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006021:Glycophorins