Plasminogen binding sites in normal human skin.
Plasminogen is detected in the basal cell layer of the epidermis, keratinocytes can generate plasminogen activators and it is suggested that the generation of plasmin may facilitate keratinocyte division, migration and differentiation. In this study we have investigated the characteristics of plasminogen binding sites in normal human epidermis. It was found that 6-aminohexanoic acid and benzamidine displaced endogenous epidermal plasminogen from the basal layer suggesting that endogenous plasminogen binds initially via the kringle 5 aminohexyl (AH) site. Plasminogen binding sites in epidermis were further investigated by displacing endogenous plasminogen and incubating sections with exogenously added glu-plasminogen, lys-plasminogen and plasmin or the isolated plasminogen fragments kringles 1-3, kringle 4 and kringle 5L. The results suggest that the uptake of plasminogen involves primary interaction with the kringle 5AH site and a secondary interaction with lysine binding sites of kringles 1-3. Cell binding is not dependent upon additional reactions of the plasmin active centre.
Burge
S M
SM
Department of Dermatology, Slade Hospital, Oxford, U.K.
Marshall
J M
JM
Cederholm-Williams
S A
SA
D015119:Aminocaproic Acid / Q000494:pharmacology; D001120:Arginine / Q000494:pharmacology; D001550:Benzamidines / Q000494:pharmacology; D005341:Fibrinolysin / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D010958:Plasminogen / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D055293:Receptors, Urokinase Plasminogen Activator; D012867:Skin / Q000187:drug effects / Q000201:enzymology*
D001550:Benzamidines; D005732:Gangliosides; C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D010958:Plasminogen; D001120:Arginine; D005341:Fibrinolysin; C032157:benzamidine; D015119:Aminocaproic Acid