Analysis of erythromycin estolate by liquid chromatography.
A method is described for the determination of erythromycin estolate by liquid chromatography. A C18 reversed-phase column (25 x 0.46 cm i.d.) was used with acetonitrile-tetrabutylammonium sulphate (pH 6.5, 0.2 M)-phosphate buffer (pH 6.5, 0.2 M)-water [x:5:5:(90-x), v/v/v/v] as mobile phase. The proportion of acetonitrile (x) has to be adapted to the type of stationary phase used. For RSil C18 LL 42.5% (v/v) was used. The column was heated at 35 degrees C, the flow rate was 1.5 ml min-1 and UV detection was performed at 215 nm. The main component, erythromycin A propionate, was separated from all other components which were present in commercial samples. The impurities most frequently observed were the propionate ester of erythromycin C and the amide N-propionyl-N-demethyl-erythromycin A. Erythromycin A was shown to be present in specialties.
Cachet
T
T
Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Chemie, Instituut voor Farmaceutische Wetenschappen, Belgium.
Delrue
M
M
Paesen
J
J
Busson
R
R
Roets
E
E
Hoogmartens
J
J
D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004918:Erythromycin Estolate / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D000097:Acetonitriles; D004918:Erythromycin Estolate; C032159:acetonitrile