PCR-assisted localization of the human SRPR gene.
A polymerase chain reaction (PCR)-based assay has been designed that detects the presence of the human signal recognition particle receptor (SRPR) gene in inter-species somatic cell hybrids. By using hybrids containing various fragments of chromosome 11q, SRPR has been mapped to a chromosomal region flanked by the 11q23 and 11q24 breakpoints associated with the constitutional and neuroepithelioma (11;22) translocations, respectively.
Janin
N
N
URA 1156 CNRS, Institut Gustave Roussy, Villejuif, France.
Delattre
O
O
Lipinski
M
M
D001483:Base Sequence; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D002892:Chromosomes, Human, Pair 22; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000235:genetics; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012261:Ribonucleoproteins / Q000235:genetics*; D018271:Signal Recognition Particle; D014178:Translocation, Genetic
D009838:Oligodeoxyribonucleotides; D012261:Ribonucleoproteins; D018271:Signal Recognition Particle; D004247:DNA