title: Differential immunoreactivity of her-2/neu oncoprotein in prostatic tissues.
abstract: To investigate HER-2/neu oncoprotein immunoreactivity, monoclonal antibody TA1 immunohistochemical examination of flash-frozen radical prostatectomy specimens was performed (n = 35). All prostatic specimens contained benign prostatic hyperplasia (BPH) and/or prostatic intraepithelial neoplasia (PIN), as well as prostatic carcinoma (CaP). HER-2/neu oncoprotein immunoreactivity in BPH tissues was not significantly different than that for the PIN basal cell layer (P = 0.10) or for the PIN luminal cells (P = 0.17). There was significantly more HER-2/neu oncoprotein immunoreactivity in BPH than in areas of CaP (P < 0.001). There was no significant difference in the amount of immunoreactivity present in PIN basal cells when compared to the PIN luminal cells (P = 0.49). Both the PIN basal cells and luminal cells stained for the HER-2/neu oncoprotein to a higher degree than cells in the CaP areas (P < 0.001 in both cases). HER-2/neu oncoprotein immunoreactivity is present at a significantly higher degree in BPH and PIN than in malignant prostatic epithelium.
authors.affiliation: Division of Urology, Duke University Medical Center, Durham, NC 27710.
authors.forename: G K
authors.initials: GK
authors.lastname: Ibrahim
authors.forename: J A
authors.initials: JA
authors.lastname: MacDonald
authors.forename: B J
authors.initials: BJ
authors.lastname: Kerns
authors.forename: S N
authors.initials: SN
authors.lastname: Ibrahim
authors.forename: P A
authors.initials: PA
authors.lastname: Humphrey
authors.forename: C N
authors.initials: CN
authors.lastname: Robertson
mesh_terms: D000368:Aged; D002277:Carcinoma / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D011467:Prostate / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases
chemical_list: D009856:Oncogene Proteins, Viral; D018719:Erb-b2 Receptor Tyrosine Kinases