title: RNA binding characteristics of a 16 kDa glycine-rich protein from maize.
abstract: We have previously described a developmentally regulated mRNA in maize that accumulates in mature embryos and is involved in a variety of stress responses in the plant. The sequence of the encoded 16 kDa protein (MA16) predicts that it is an RNA-binding protein, since it possesses a ribonucleoprotein consensus sequence-type RNA-binding domain (CS-RBD). To assess the predicted RNA binding property of the protein and as a starting point to characterize its function we have used ribohomopolymer-binding assays. Here we show that the MA16-encoded protein binds preferentially to uridine- and guanosine-rich RNAs. In light of these results a likely role for this protein in RNA metabolism during late embryogenesis and in the stress response is discussed.
authors.affiliation: Departamento de Genética Molecular, Centro de Investigación y Desarrollo, Barcelona, Spain.
authors.forename: M D
authors.initials: MD
authors.lastname: Ludevid
authors.forename: M A
authors.initials: MA
authors.lastname: Freire
authors.forename: J
authors.initials: J
authors.lastname: Gómez
authors.forename: C G
authors.initials: CG
authors.lastname: Burd
authors.forename: F
authors.initials: F
authors.lastname: Albericio
authors.forename: E
authors.initials: E
authors.lastname: Giralt
authors.forename: G
authors.initials: G
authors.lastname: Dreyfuss
authors.forename: M
authors.initials: M
authors.lastname: Pagès
mesh_terms: D000595:Amino Acid Sequence; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D011066:Poly C / Q000378:metabolism; D011068:Poly G / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D003313:Zea mays / Q000235:genetics*
chemical_list: D006360:Heat-Shock Proteins; D010940:Plant Proteins; D016601:RNA-Binding Proteins; C073976:GRP protein, Zea mays; D011061:Poly A; D011068:Poly G; D011072:Poly U; D011066:Poly C