Solid phase peptide synthesis on hydrophilic supports. Part II--Studies using Perloza beaded cellulose.
Beaded cellulose (Perloza) was modified with acrylonitrile followed by reduction with diborane to give a functionalised support containing aminopropyl groups. This spacer arm was then further extended with a glycolamido or an Fmoc-amino acid-4-oxymethylphenoxyacetyl moiety. A number of peptides, including the Merrifield test peptide leucyl-alanyl-glycyl-valine, leucine-enkephalin, Acyl Carrier Protein (65-74), angiotensin I and II, ACTH(4-11) and LHRH were synthesised on the aminopropyl beaded cellulose support using modified t-butyloxycarbonyl (Boc) or fluorenylmethoxycarbonyl (Fmoc) synthesis protocols. The peptides were cleaved from the support and further purified.
Englebretsen
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Separation Science Unit, Massey University, Palmerston North, New Zealand.
Harding
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D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D002482:Cellulose; D004126:Dimethylformamide / Q000737:chemistry; D004146:Dioxanes / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry; D005563:Formic Acid Esters / Q000737:chemistry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry; D010316:Particle Size; D010455:Peptides / Q000138:chemical synthesis*
D000596:Amino Acids; D004146:Dioxanes; D005449:Fluorenes; D005563:Formic Acid Esters; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D009842:Oligopeptides; D010455:Peptides; C000345:t-butyloxycarbonyl group; C079675:leucyl-alanyl-glycyl-valine; D004126:Dimethylformamide; D002482:Cellulose; C025223:1,4-dioxane