[The permeability of the barrier of the gastrointestinal tract for the macromolecules of polyethylene glycol-4000: an assessment of the mechanism and its reproducibility].
Polyethylene glycol (PEG)-4000 is proposed as a tracer of intestinal macromolecular permeability. The reproducibility of permeability testing with PEG-4000 and the mechanism of its penetration through intestinal mucosa were studied in adult rats. Permeability measurement for PEG-4000 was reproducible when repeated twice for 2 days. This makes it possible to repeat PEG-4000 permeability testing before and after any experimental impact on the intestine. I.p. administration of colchicine to rats (125 micrograms/100 g b. w.) significantly inhibited intestinal absorption of PEG-4000 fed to the animals 3 hours later. Hence, PEG-4000 penetration through the intestinal mucosa is mediated by the system of enterocyte cytoplasmic microtubules. Mucosal permeability for PEG-4000 may be consequently considered as a valuable model of permeability for protein macromolecules.
Gmoshinskiĭ
I V
IV
Verina
T Iu
TIu
Mazo
V K
VK
Morozov
I A
IA
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003078:Colchicine / Q000494:pharmacology; D004064:Digestive System / Q000187:drug effects* / Q000378:metabolism; D004231:Diuresis / Q000187:drug effects; D007408:Intestinal Absorption / Q000187:drug effects; D046911:Macromolecular Substances; D008297:Male; D011092:Polyethylene Glycols / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
D046911:Macromolecular Substances; D011092:Polyethylene Glycols; D003078:Colchicine
Pronitsaemost' bar'era zheludochno-kishechnogo trakta dlia makromolekul poliétilenglikolia-4000: otsenka mekhanizma i vosproizvodimosti.