Transbronchial biopsy in sarcoidosis: the role of immunohistochemical analysis for granuloma detection.
In this study 27 transbronchial biopsy specimens obtained from patients with clinical and/or laboratory features suggestive for sarcoidosis were analysed with conventional morphology and immunohistochemistry comparing the sensitivity and reproducibility of the two methods. A limited panel of monoclonal antibodies recognizing epitopes resistant to conventional fixation and embedment procedures were used (CD45R0, CD68, anti-cheratin, anti-collagen IV). All specimens were independently observed by two different pathologists. On the basis of the recognition of bona-fide noncaseating granulomas, 9 cases were uniformly judged as positive, 10 cases as negative, but 8 cases were considered doubtful. Immunohistochemical analysis reliably demonstrated the presence of epithelioid cells in 3 of these cases and absence in 5. These data suggest that the use of a limited immunohistochemical analysis can significantly improve histological diagnosis of sarcoidosis on small transbronchial biopsies using conventional routine material.
Menestrina
F
F
Istituto di Anatomia Patologica, Università di Verona, Italy.
Lestani
M
M
Mombello
A
A
Cipriani
A
A
Pomponi
F
F
Adami
F
F
Semenzato
G
G
Chilosi
M
M
D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001706:Biopsy; D003094:Collagen / Q000032:analysis; D006099:Granuloma / Q000175:diagnosis / Q000276:immunology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D017493:Leukocyte Common Antigens / Q000032:analysis; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008171:Lung Diseases / Q000175:diagnosis / Q000276:immunology / Q000473:pathology; D015203:Reproducibility of Results; D017565:Sarcoidosis, Pulmonary / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D012680:Sensitivity and Specificity; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D003094:Collagen; D007633:Keratins; D017493:Leukocyte Common Antigens; C067980:CD68 antigen, human; D000097382:CD68 Molecule