The regulation of connective tissue metabolism by vasoactive intestinal polypeptide.
Immunohistochemical studies have confirmed the innervation of bone with neuropeptidergic neurons containing vasoactive intestinal polypeptide (VIP), substance P (SP) and calcitonin gene-related peptide (CGRP). In this study, we report effects of VIP on connective tissue cell metabolism. VIP stimulated PGE2 production in human articular chondrocytes, human osteoblast-like cells and human synovial cells, however, stromelysin production was unaffected. VIP also stimulated cAMP production in human osteoblast-like cells, but not in human articular chondrocytes or synovial cells. These findings are suggestive of a role of VIP in connective tissue cell metabolism which may contribute to the inflammatory processes of arthritis.
Rahman
S
S
Department of Human Metabolism, University of Sheffield Medical School, U.K.
Dobson
P R
PR
Bunning
R A
RA
Russell
R G
RG
Brown
B L
BL
D002358:Cartilage, Articular / Q000378:metabolism; D003238:Connective Tissue / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000096:biosynthesis; D010006:Osteoblasts / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D013583:Synovial Membrane / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP; D010447:Peptide Hydrolases; D008666:Metalloendopeptidases; C030895:caseinase; D019278:Matrix Metalloproteinase 3; D015232:Dinoprostone