[The regulation of the DNA repair process in mammalian cells. IV. The role of DNA polymerases in the epidermal growth factor regulation of the repair of single-stranded DNA breaks induced by ionizing radiation in Swiss 3T6 mouse cells].
A study was made of the repair of ionizing radiation-induced DNA single-strand breaks (SSB) in proliferating and quiescent mouse Swiss 3T6 cells and in those stimulated from the quiet status by epidermal growth factor in combination with insulin, in the presence of specific inhibitors of DNA polymerase alpha and delta (aphidicolin) and DNA polymerase beta (2', 3'-dideoxythymidine-5'-triphosphate). The repair of DNA SSB induced by X-ray-irradiation (10 Gr) or by gamma-ray irradiation (150 Gr) is more sensitive to aphidicolin independently of cell proliferating status. Aphidicolin inhibits the recovery of single-strand DNA in quiescent and mitogen-stimulated cells three times stronger than in proliferating cells. The influence of 2', 3'-dideoxythymidine-5'-triphosphate on the rate of DNA SSB repair in cells of all the three types does not differ. Thus, the decrease in DNA repair efficiency in quiescent cells is connected with a decrease in the activity of aphidicolin-sensitive DNA polymerase, apparently DNA polymerase alpha. It is suggested that the regulation action of mitogens on the DNA SSB repair may be determined by qualitative changes of this enzyme or of some conditions in which it functions. The involvement of DNA polymerase delta in this process is not excluded.
Bil'din
V N
VN
Seregina
T B
TB
Zhestianikov
B D
BD
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects / Q000502:physiology; D004277:DNA, Single-Stranded / Q000187:drug effects / Q000528:radiation effects*; D004259:DNA-Directed DNA Polymerase / Q000502:physiology*; D054306:Dideoxynucleotides; D004347:Drug Interactions; D004815:Epidermal Growth Factor / Q000494:pharmacology / Q000502:physiology*; D007328:Insulin / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D019384:Nucleic Acid Synthesis Inhibitors; D013942:Thymine Nucleotides / Q000494:pharmacology
D004277:DNA, Single-Stranded; D054306:Dideoxynucleotides; D007328:Insulin; D019384:Nucleic Acid Synthesis Inhibitors; D013942:Thymine Nucleotides; D016590:Aphidicolin; D004815:Epidermal Growth Factor; D004259:DNA-Directed DNA Polymerase; C017216:2',3'-dideoxythymidine triphosphate
Reguliatsiia protsessov reparatsii DNK v kletkakh mlekopitaiushchikh. IV. Rol' DNK-polimeraz v reguliatsii épidermal'nym faktorom rosta reparatsii odnonitevykh razryvov DNK, indutsirovannykh ioniziruiushcheĭ radiatsieĭ, v myshinykh kletkakh Swiss 3T6.