title: Induction of Mn-superoxide dismutase by tumor necrosis factor, interleukin-1 and interleukin-6 in human hepatoma cells.
abstract: Effects of Tumor Necrosis Factor (TNF), Interleukin-1 (IL-1), Interleukin-6 (IL-6) and Interferon-gamma (IFN-gamma) on the expression of Mn-superoxide dismutase (Mn-SOD) protein were investigated in human hepatoma cells, Hu-H1, which revealed resistance to the cytotoxicity of TNF and IL-1. Both TNF and IL-1 enhanced the Mn-SOD production to the level of 30- to 40-fold. IL-6 also increased the enzyme protein to 2- to 3-fold of the basal level without any cell proliferative effect. A specific antibody against IL-6 almost completely inhibited the induction of Mn-SOD. IL-6, as well as TNF and IL-1, appears to play some role in the Mn-SOD protein expression in human hepatoma cells.
authors.affiliation: Department of Internal Medicine III, Asahikawa Medical College, Hokkaido, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ono
authors.forename: H
authors.initials: H
authors.lastname: Kohda
authors.forename: T
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: M
authors.initials: M
authors.lastname: Ohhira
authors.forename: C
authors.initials: C
authors.lastname: Sekiya
authors.forename: M
authors.initials: M
authors.lastname: Namiki
authors.forename: A
authors.initials: A
authors.lastname: Takeyasu
authors.forename: N
authors.initials: N
authors.lastname: Taniguchi
mesh_terms: D000906:Antibodies; D006528:Carcinoma, Hepatocellular; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D004790:Enzyme Induction; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D007700:Kinetics; D008113:Liver Neoplasms; D011994:Recombinant Proteins / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000096:biosynthesis*; D013936:Thymidine / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D000906:Antibodies; D007375:Interleukin-1; D015850:Interleukin-6; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D013482:Superoxide Dismutase; D013936:Thymidine