Cross-ligation and exchange reaction of RNA catalyzed by hairpin ribozymes.
The catalytic domain in the minus strand of the satellite RNA of tobacco ringspot virus (sTobRV(-)) assumes a hairpin-like secondary structure. This ribozyme catalyzes a cross-ligation reaction between substrate RNAs of different lengths. We constructed ribozymes to probe the activities of ligation and RNA fragment exchange.
Komatsu
Y
Y
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Koizumi
M
M
Sekiguchi
A
A
Ohtsuka
E
E
D001483:Base Sequence; D002384:Catalysis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010942:Plant Viruses / Q000235:genetics; D012313:RNA / Q000378:metabolism; D016337:RNA, Catalytic / Q000378:metabolism*; D019244:RNA, Satellite; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*
D016337:RNA, Catalytic; D019244:RNA, Satellite; D012367:RNA, Viral; D012313:RNA