Detection of creatine kinase isoenzymes as tumoral markers of rhabdomyosarcoma.
We analyzed the expression profile of isoenzymatic fractions of creatine phosphokinase (EC 2.7.3.2) isotypes MM, MB and BB in three cell lines derived from embryonic rhabdomyosarcomas and a normal counterpart cell line. Electrophoretic data showed that the BB fraction was consistently expressed de novo, in contrast with its counterpart in normal tissue. The BB fraction may serve as new tumoral marker for the diagnosis of rhabdomyosarcoma. In addition, the appearance of macrocreatine kinase type-1 in this type of neoplasm may serve to reinforce the diagnosis when rhabdomyosarcoma is suspected.
Fernández
J E
JE
Department of Morphological Sciences, School of Medicine, University of Granada, Spain.
Rodríguez
F
F
Recio
M A
MA
Vélez
C
C
Aránega
A E
AE
Alvarez
L
L
Aránega
A
A
D014408:Biomarkers, Tumor / Q000032:analysis*; D003402:Creatine Kinase / Q000032:analysis*; D006801:Humans; D007527:Isoenzymes; D009132:Muscles / Q000201:enzymology*; D012016:Reference Values; D012208:Rhabdomyosarcoma / Q000201:enzymology*; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D007527:Isoenzymes; D003402:Creatine Kinase