Radioimmunodetection and autoradiographic localization of monoclonal antibody against human hepatocellular carcinoma in xenografts.
The monoclonal antibody against human hepatocellular carcinoma, HAb23, was conjugated with radioiodine and injected into nude mice with human hepatoma xenografts. After 4 to 5 days, the nude mice were scanned by emission computerized topography. The results showed that the xenografts were distinguished clearly. The tumor-liver and tumor-blood ratios were 1.94 to 3.66 and 0.56 to 1.65, respectively. Autoradiography showed that the HAb23 was localized mainly on the surface membrane and tubulovesicular systems of the tumor cells.
Ji
Y Y
YY
Department of Pathology, Fourth Military Medical University, Shaanxi, People's Republic of China.
Liu
Y F
YF
Chen
Z N
ZN
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000912:Antibodies, Neoplasm / Q000032:analysis*; D001345:Autoradiography; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D006801:Humans; D007457:Iodine Radioisotopes; D008113:Liver Neoplasms / Q000276:immunology*; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation / Q000000981:diagnostic imaging / Q000276:immunology; D016719:Radioimmunodetection
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007457:Iodine Radioisotopes