Newly synthesized class II MHC chains are required for VSV G presentation to CTL clones.
Mechanisms of antigen processing and presentation to thymus-derived lymphocytes have been under intense study for several years, focusing on both Class I-restricted antigen presentation and Class II-MHC restricted responses. The studies described here examine the processing and presentation of exogenously provided soluble glycoprotein of the vesicular stomatitis virus and as well as newly synthesized viral glycoprotein. Evidence is provided that newly synthesized Class II MHC chains are required for cell surface expression of processed glycoprotein determinants irrespective of the origin of the viral antigen. Inhibitors of distinct cellular processes, including ammonium chloride, emetine, and Brefeldin A, have been used to dissect the pathways utilized.
Reiss
C S
CS
Department of Pathology, Harvard Medical School, Boston, Massachusetts.
Gapud
C P
CP
Keil
W
W
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D020126:Brefeldin A; D002738:Chloroquine / Q000494:pharmacology; D002999:Clone Cells; D003517:Cyclopentanes / Q000494:pharmacology; D004640:Emetine / Q000494:pharmacology; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000276:immunology*; D008211:Lymphocyte Cooperation; D008562:Membrane Glycoproteins; D051379:Mice; D011994:Recombinant Proteins; D012995:Solubility; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
D000956:Antigens, Viral; D003517:Cyclopentanes; C028391:G protein, vesicular stomatitis virus; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D000643:Ammonium Chloride; D020126:Brefeldin A; D002738:Chloroquine; D004640:Emetine