A high performance liquid chromatographic method for the determination of albuterol enantiomers in human serum using solid phase extraction and chemical derivatization.
A high performance liquid chromatographic method was developed for the simultaneous assay of R(-)- and S(+)-albuterol in human serum. The assay involves solid phase extraction as a sample clean-up step and derivatization of racemic albuterol to its diastereomeric thioureas with 2,3,4,6-tetra-O-acetyl-alpha-D-glucopyranosyl isothiocyanate. Chromatographic separation was accomplished under isocratic conditions using an octadecylsilane column and a mobile phase consisting of 29:71 acetonitrile:distilled water containing 0.1% triethylamine, pH 4.0 (adjusted with concentrated phosphoric acid) at a flow rate of 0.8 mL/min. The diastereomers were detected using a fluorescence detector set at 223 nm excitation and no emission filter. Racemic bamethane was used as internal standard. Drug to internal standard peak-height ratios were linear over a 2-20 ng/mL range for each enantiomer. The limit of detection of each analyte was 1.0 ng/mL (S/N = 3).
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Department of Medicinal Chemistry, College of Pharmacy, University of Georgia, Athens 30602.
Stewart
J T
JT
D000420:Albuterol / Q000097:blood* / Q000493:pharmacokinetics; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D017879:Isothiocyanates; D008752:Methylene Chloride; D013237:Stereoisomerism; D013861:Thiocyanates
D007202:Indicators and Reagents; D017879:Isothiocyanates; D013861:Thiocyanates; C041943:2,3,4,6-tetra-O-acetylglucopyranosylisothiocyanate; D008752:Methylene Chloride; D000420:Albuterol