Reconstitution of denatured E. coli alkaline phosphatase with E. coli ribosome.
E. coli alkaline phosphatase was denatured by physical/chemical means. In vitro reconstitution of this denatured enzyme was assisted by 70S E. coli ribosome, as shown by the recovery of its catalytic competence. Almost total recovery of activity of the totally inactivated enzyme was obtained in presence of equimolar concentration of 70S ribosome at 50 degrees C.
Das
B
B
Department of Biophysics, Molecular Biology and Genetics, University of Calcutta.
Gupta
C D
CD
D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D011489:Protein Denaturation; D017510:Protein Folding; D012270:Ribosomes / Q000378:metabolism*; D013816:Thermodynamics
D000469:Alkaline Phosphatase