title: Actions of thiocyanate and N-phenylmaleimide on volume-responsive Na and K transport in dog red cells.
abstract: Two sets of observations suggest a linkage between volume-responsive Na and K transport systems in dog red blood cells. 1) The lyotropic anion thiocyanate inhibits shrinkage-induced Na-H exchange and stimulates swelling-induced K-Cl cotransport. 2) The effect of a brief incubation with N-phenylmaleimide (NPM) on Na and K transport depends on the volume of the cells at the time of exposure to the sulfhydryl reagent. Cells shrunken during the NPM incubation and then brought back to normal volume behave as though they were still shrunken, i.e., they show an increased Na flux and a decreased K flux. Cells incubated with NPM in a swollen state retain fluxes characteristic of swollen cells when returned to a normal volume. The electrophoretic mobility of the membrane-associated enzyme glyceraldehyde-3-phosphate dehydrogenase is influenced by the cell volume at the time of NPM exposure. These findings point to the existence of a system in cells that perceives volume changes and coordinates the responses of membrane transporters.
authors.affiliation: Department of Medicine, University of North Carolina, Chapel Hill 27599.
authors.forename: J C
authors.initials: JC
authors.lastname: Parker
authors.forename: G C
authors.initials: GC
authors.lastname: Colclasure
mesh_terms: D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D003470:Culture Media; D004285:Dogs; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D008301:Maleimides / Q000494:pharmacology*; D011188:Potassium / Q000097:blood; D012964:Sodium / Q000097:blood*; D017923:Sodium-Hydrogen Exchangers; D027981:Symporters; D013861:Thiocyanates / Q000494:pharmacology*; D000096922:K Cl- Cotransporters
chemical_list: D002352:Carrier Proteins; D003470:Culture Media; D008301:Maleimides; D011188:Potassium; D012964:Sodium; D017923:Sodium-Hydrogen Exchangers; D027981:Symporters; D013861:Thiocyanates; D000096922:K Cl- Cotransporters; C028525:N-phenylmaleimide; C031760:thiocyanate