title: Chromatographic separation of serum proteins and estimation of their zinc and copper content.
abstract: Human serum proteins of blood donors and dialysis patients were separated by means of gel filtration chromatography. The resulting fractions were analyzed for copper and zinc. Separation resulted in 3 zinc peaks with a molecular weight of about 700,000, 300,000, and 75,000 Dalton, with alpha 2-macroglobulin co-eluting in the first and albumin co-eluting in the third zinc peak. The zinc protein(s) of the second peak remained unidentified. The three peaks contained, in succession, 0.72 +/- 0.30 mumol/L (4.8 +/- 1.6%), 1.26 +/- 0.37 mumol/L (8.5 +/- 1.7/1000) and 12.8 +/- 2.1 mumol/L (86.8 +/- 2.8%) of total zinc in the case of blood donors, and 1.19 +/- 1.05 mumol/L (9.2 +/- 7.2%), 0.97 +/- 0.22 mumol/L (8.0 +/- 2.6%), and 10.4 +/- 1.66 mumol/L (82.7 +/- 6.7%) in the case of dialysis patients. Separation followed by copper analysis resulted in the three peaks, as well, with a molecular weight of about 750,000, 140,000, and 75,000 dalton. The copper protein of the first peak remained unidentified, while coeruloplasmin co-eluted in the second and albumin in the third peak. The three peaks contained, in succession, 0.4 +/- 0.16 mumol/L (2.3 +/- 0.95%), 14.6 +/- 0.7 mumol/L (83.9 +/- 4.1%), and 2.4 +/- 0.6 mumol/L (13.7 +/- 3.5%) of total copper in the case of blood donors, and 0.5 +/- 0.73 mumol/L (2.2 +/- 3.2%), 19.5 +/- 1.1 mumol/L (90.5 +/- 4.9%), and 1.6 +/- 0.66 mumol/L (7.3 +/- 3.0/1000) in the case of dialysis patients. Limitation of the method is shown regarding separation of major from minor proteins and albumin from transferrin.
authors.affiliation: Institut für Klinische Chemie, Katharinenhospital, Stuttgart, Fed. Rep. of Germany.
authors.forename: U
authors.initials: U
authors.lastname: Gless
authors.forename: Y
authors.initials: Y
authors.lastname: Schmitt
authors.forename: S
authors.initials: S
authors.lastname: Ziegler
authors.forename: J D
authors.initials: JD
authors.lastname: Kruse-Jarres
mesh_terms: D001782:Blood Donors; D001798:Blood Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002850:Chromatography, Gel; D003300:Copper / Q000032:analysis*; D006801:Humans; D008970:Molecular Weight; D006435:Renal Dialysis; D015032:Zinc / Q000032:analysis*
chemical_list: D001798:Blood Proteins; D003300:Copper; D015032:Zinc