Frequent deletions in nine newly immortal human cell lines.
Nine newly immortal lines of human fibroblasts transfected with SV40 T antigen were examined for recurrent chromosome losses. In order of decreasing frequency, all nine lines had three or more of the following minimal deletions specifically associated with the immortalization event: del(6)(q21), del(3)(p24), del(1)(p34), del(4)(p25), del(5)(p14), del(11)(p11), del(11)(q14), del(12)(p12), and del(14)(p?). Many other chromosome changes were not clearly associated with immortalization, but were acquired during other stages of this multistep model of neoplastic transformation. We propose that these chromosome loci associated with immortalization are candidates for the location of genes involved in cellular senescence.
Ray
F A
FA
Cellular and Molecular Biology Group, Los Alamos National Laboratory, New Mexico.
Kraemer
P M
PM
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D000090266:Chromobox Protein Homolog 5; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D025063:Chromosome Disorders; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007621:Karyotyping; D008297:Male; D010957:Plasmids; D011003:Ploidies; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology; D012867:Skin / Q000166:cytology
D000952:Antigens, Polyomavirus Transforming; C000715594:CBX5 protein, human; D000090266:Chromobox Protein Homolog 5