Analysis of retroviral proteins by two-step DNA, zinc binding, and modified Southern-western blotting.
Using Southern-Western and zinc-blotting techniques, the interactions of single-stranded DNA and zinc with structural proteins of type B, C, and D retroviruses were examined. Besides nucleic capsid proteins of retroviruses known to form complexes with nucleic acids, some other core proteins of type C retroviruses were shown to bind nucleic acids. All nucleic capsid proteins of the examined retroviruses appeared to be also zinc binding. In the present report, we propose two protein-blotting techniques that could be performed on a single nitrocellulose membrane. A first technique allows to detect zinc- and DNA-binding proteins immobilized on the membrane; a second (a modification of Southern-Western blotting) makes it possible to detect DNA-binding proteins followed by immunological reprobing.
Morozov
V A
VA
Laboratory of Cancer Virus Immunology, Russian Academy of Medical Sciences, Moscow.
Lagaye
S
S
Ilyinskii
P O
PO
Cherepovsky
D N
DN
Ilyin
K V
KV
D015139:Blotting, Southern / Q000379:methods*; D015153:Blotting, Western / Q000379:methods*; D004268:DNA-Binding Proteins / Q000032:analysis; D012190:Retroviridae / Q000737:chemistry*; D014764:Viral Proteins / Q000032:analysis*; D015032:Zinc / Q000378:metabolism; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D014764:Viral Proteins; D015032:Zinc