Stimulation of Nb2 mitogenesis by an analogue of human growth hormone (110-127).
Two 18-mer peptides were synthesized. Peptide E117 had the same amino acid sequence as that in the region 110-127 of human growth hormone [hGH (110-127)]. In the second peptide (L117), E117 was replaced with leucine. Biological activity of the peptides was assessed in cultured Nb2 lymphoma cells. L117 stimulated cell division and DNA synthesis in a concentration-dependent manner. Maximum stimulation was equivalent to that noted with native hGH although L117 was much less potent (L117; EC50 approximately 1 microM vs. hGH; EC50 approximately 20 pM). No demonstrable activity was found with peptide E117. These results suggest that the amphipathic helical structure of hGH (110-127) is crucial for biological activity.
Kornberg
L J
LJ
Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University.
Liberti
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D013006:Growth Hormone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D016399:Lymphoma, T-Cell; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D051381:Rats; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; C079622:E 117 peptide; C079623:L 117 peptide; D010446:Peptide Fragments; D013006:Growth Hormone