title: Evaluation of the E test for antimicrobial susceptibility testing of Actinobacillus actinomycetemcomitans.
abstract: The E test was compared with an agar dilution method for antimicrobial susceptibility testing of Actinobacillus actinomycetemcomitans on Mueller-Hinton Haemophilus test medium. Eighty recent isolates of A. actinomycetemcomitans from 40 subjects and 3 reference strains were studied. Excellent agreement was obtained between the E test and the agar dilution test for benzylpenicillin (98.8%), ampicillin (97.5%), cefaclor (100%), cefuroxime (100%), tetracycline hydrochloride (100%) and ciprofloxacin (98.7%); 76.0% agreement was obtained for metronidazole and only 5.2% for cotrimoxazole. This study indicates that the E test is a reliable method to determine minimal inhibitory concentrations of antimicrobials against A. actinomycetemcomitans, except for metronidazole and cotrimoxazole.
authors.affiliation: Department of Periodontology, National Public Health Institute, Helsinki, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Pajukanta
authors.forename: S
authors.initials: S
authors.lastname: Asikainen
authors.forename: M
authors.initials: M
authors.lastname: Saarela
authors.forename: S
authors.initials: S
authors.lastname: Alaluusua
authors.forename: H
authors.initials: H
authors.lastname: Jousimies-Somer
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents