title: In vitro influence of oxygenation on enzymes of rat adipose tissue.
abstract: Pre-incubation of rat adipose tissue from ad lib fed rats in the presence of 1.2 U/ml insulin and 2.8 x 10(-7) M prostaglandin F2 alpha for 16 hours in a gas atmosphere of 5% O2 led to an increase in glucose-6-phosphate dehydrogenase activity. No increase in glycerol-3-phosphate dehydrogenase or hexokinase activities were noted. Actinomycin D (50 micrograms/ml) and dehydroepiandrosterone (120 micrograms/ml) attenuated this increase and the increase in incorporation of [1-14C] glucose into carbon dioxide and tissue seen under these conditions. No increase in glucose-6-phosphate dehydrogenase was seen when pre-incubation was made in a gas atmosphere containing 20% O2. Although dehydroepiandrosterone inhibited both enzyme activity and isotope incorporation in a 20% O2 gas atmosphere, actinomycin D was without effect.
authors.affiliation: Department of Chemical Pathology, Faculty of Medicine, University of Stellenbosch, Tygerberg Hospital, Republic of South Africa.
authors.forename: C M
authors.initials: CM
authors.lastname: Macfarlane
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D002478:Cells, Cultured; D003609:Dactinomycin / Q000494:pharmacology; D003687:Dehydroepiandrosterone / Q000494:pharmacology; D015237:Dinoprost / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats
chemical_list: D007328:Insulin; D003609:Dactinomycin; D003687:Dehydroepiandrosterone; D015237:Dinoprost; D005993:Glycerolphosphate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D010100:Oxygen