title: [Use of cytogenetic and molecular biology in the detection of chronic myeloid leukemia].
abstract: The examination of the presence of Ph chromosome and of the fused gene BCR-ABL in patients with chronic myeloid leukemia (CML) is significant for the precise diagnosis and in some cases for the prognosis of the disease. We examined peripheral blood for the presence of BCR-ABL fused gene by polymerase chain reaction (PCR) in eight patients with CML consecutively cytogenetically studied before and after the bone marrow transplantation and in two patients treated with interferon. Southern blot analysis was performed before BMT in two patients and the molecular rearrangement of Ph chromosome was found. In all cases our results have proved that cytogenetic and recombinant DNA evaluations confirm each other. Due to the high sensitivity of PCR technique the minimal residual leukemia can be detected.
authors.affiliation: III. interní klinika 1. lékarské fakulty Univerzity Karlovy, Praha.
authors.forename: K
authors.initials: K
authors.lastname: Michalová
authors.forename: Z
authors.initials: Z
authors.lastname: Zemanová
authors.forename: C
authors.initials: C
authors.lastname: Haskovec
authors.forename: B
authors.initials: B
authors.lastname: Rypácková
authors.forename: R
authors.initials: R
authors.lastname: Brdicka
mesh_terms: D015139:Blotting, Southern; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000235:genetics; D010677:Philadelphia Chromosome; D016133:Polymerase Chain Reaction
vernacular_title: Cytogenetický a molekulárnĕ biologický průkaz chronické myeloidní leukémie.