title: The biological interaction of cis- and trans-urocanic acid and DNA.
abstract: The potential for the cis and trans isomers of urocanic acid to produce DNA damage was measured by assays for DNA binding (32P-postlabeling assay), for induction of DNA repair (unscheduled DNA synthesis assay) and induction of mutations (Salmonella typhimurium and Escherichia coli plate-incorporation assays). These assays did not detect any evidence of a direct effect of either isomer of urocanic acid on DNA over a wide range of concentrations. These results suggest that neither isomer of urocanic acid alone, nor ultraviolet-irradiation of either cis or trans-urocanic acid produces significant DNA damage under conditions that permit cell survival.
authors.affiliation: Applied Genetics Inc., Freeport, New York 11520.
authors.forename: D B
authors.initials: DB
authors.lastname: Yarosh
authors.forename: S D
authors.initials: SD
authors.lastname: Gettings
authors.forename: L G
authors.initials: LG
authors.lastname: Alas
authors.forename: J T
authors.initials: JT
authors.lastname: Kibitel
authors.forename: R H
authors.initials: RH
authors.lastname: San
authors.forename: V O
authors.initials: VO
authors.lastname: Wagner
authors.forename: G N
authors.initials: GN
authors.lastname: McEwen
mesh_terms: D000818:Animals; D002417:Cattle; D002461:Cell Line, Transformed; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000187:drug effects; D004926:Escherichia coli; D005347:Fibroblasts; D006801:Humans; D008297:Male; D008862:Microsomes, Liver; D009154:Mutation / Q000187:drug effects; D010761:Phosphorus Radioisotopes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012486:Salmonella typhimurium; D013237:Stereoisomerism; D014466:Ultraviolet Rays; D014560:Urocanic Acid / Q000494:pharmacology*
chemical_list: D004269:DNA, Bacterial; D010761:Phosphorus Radioisotopes; D004247:DNA; D014560:Urocanic Acid