Complete coverage of the Schizosaccharomyces pombe genome in yeast artificial chromosomes.
The genome of the fission yeast, Schizosaccharomyces pombe, consists of some 14 million base pairs of DNA contained in three chromosomes. On account of its excellent genetics we used it as a test system for a strategy designed to map mammalian chromosomes and genomes. Data obtained from hybridization fingerprinting established an ordered library of 1,248 yeast artificial chromosome clones with an average size of 535 kilobases. The clones fall into three contigs completely representing the three chromosomes of the organism. This work provides a high resolution physical and clone map of the genome, which has been related to available genetic and physical map information.
Maier
E
E
Genome Analysis Laboratory, Imperial Cancer Research Fund, Lincolns Inn Fields, London, UK.
Hoheisel
J D
JD
McCarthy
L
L
Mott
R
R
Grigoriev
A V
AV
Monaco
A P
AP
Larin
Z
Z
Lehrach
H
H
D001482:Base Composition; D005075:Biological Evolution; D002678:Chimera; D002874:Chromosome Mapping / Q000379:methods; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D015723:Gene Library; D016681:Genome, Fungal; D006801:Humans; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces
D004271:DNA, Fungal; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases