[Comparative study on antigenicity and immunogenicity of the 24-26 kDa antigen and the recombinant Sj26 antigen of Schistosoma japonicum].
This paper reports on the comparison of the recombinant Sj26 (rSj26) antigen originated from the Philippine strain and 24-26 kDa antigen isolated and purified from Chinese mainland strain of S. japonicum for their antigenicity and immunogenicity. The results showed that there were obvious cross reactions between rSj26 and 24-26 kDa antigen when rSj26 antigen was tested against specific antibodies in sera from mice infected with the mainland strain of S. japonicum or 24-26 kDa antigen was tested against specific anti-rSj26 antibodies by ELISA, IFA and Western blotting etc. Both of 24-26 kDa and rSj26 antigen had weak cross reaction with SEA antigen. The worm reduction rate after challenging with mainland strain cercariae in mice immunized with rSj26 was 26-32%, similar to that in mice immunized with 24-26 kDa antigen. It is suggested that rSj26 antigen can induce certain level of specific protective immunity to protect the host against infection by Chinese strain of S. japonicum cercaciae.
Liu
S X
SX
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Song
G C
GC
Ding
L Y
LY
Xu
Y X
YX
Wu
G Z
GZ
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology*; D015153:Blotting, Western; D003429:Cross Reactions; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*
D000947:Antigens, Helminth; D011994:Recombinant Proteins