Protein kinase C isozyme expression in human leukemia-lymphoma cell lines--an immunocytochemical study.
There have been an increasing number of reports describing a pivotal role for phosphorylation in cellular responses for cell differentiation and proliferation. We examined an immunocytochemical expression of protein kinase C(PKC) isozymes (type I, II, and III) in 22 leukemia-lymphoma cell lines. Of these cell lines, 21 expressed type II PKC and 17 showed the co-expression of both types II and III PKC in varying degree. The cell line without PKC activity showed far less [3H]-TdR uptake and no heterotransplantation in nude mice. Types II and III PKC appear to relate to cell proliferation in certain leukemia-lymphoma cell lines.
Hojo
H
H
Department of Pathology, Fukushima Medical College, Japan.
Morimura
Y
Y
Abe
M
M
Tominaga
K
K
Nozawa
Y
Y
Nakamura
N
N
Ono
N
N
Tasaki
K
K
Yamaguchi
Y
Y
Suzuki
S
S
D000911:Antibodies, Monoclonal; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D007938:Leukemia / Q000201:enzymology*; D008223:Lymphoma / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D011493:Protein Kinase C