title: Evidence against oxidant injury and endotoxin underlying glycerol-induced fatal rhabdomyolysis in rats.
abstract: Rhabdomyolysis in experimental animals and in man may be fatal. The mechanism of action causing death in man and in the experimental model of laboratory animals injected with glycerol in a dose causing fatal rhabdomyolysis is unknown. The aim of this study was to examine two possible causes of death following glycerol injection (0.5-2 ml/100 g) to the leg muscle of rats following a period of water deprivation (24-72 h). The first factor examined was free radical formation, via the administration of various antioxidants such as natural antioxidant (NAO - 5 mg/100 g), vitamin E (0.2 mg/100 g each day for 7 days), dimethylthiourea (DMTU - 50 mg/100 g) and superoxide dismutase (SOD - 0.8 mg/100 g). The second factor was a high blood endotoxin level due to the glycerol injection. This possibility was examined by glycerol injection to rats that had developed endotoxin tolerance. The low survival rate (0-30%) of rats receiving a glycerol injection following preventive treatment with antioxidants as well as rats which had developed endotoxin tolerance (0-40%), excludes the possibility that the cause of death in the glycerol model resulting in fatal rhabdomyolysis is due to free radical formation or to a high and unneutralized blood level of endotoxin.
authors.affiliation: Department of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Zurovsky
authors.forename: S
authors.initials: S
authors.lastname: Grossman
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004731:Endotoxins / Q000378:metabolism*; D005990:Glycerol / Q000633:toxicity*; D008297:Male; D016877:Oxidants / Q000378:metabolism*; D051381:Rats; D012206:Rhabdomyolysis / Q000139:chemically induced / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000494:pharmacology; D015996:Survival Rate; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D004731:Endotoxins; D016877:Oxidants; D014810:Vitamin E; C038983:1,3-dimethylthiourea; D013482:Superoxide Dismutase; D013890:Thiourea; D005990:Glycerol