A high concentration of NPY (Y1)-receptor mRNA-expressing cells in the rat arcuate nucleus.
The Y1 neuropeptide Y (NPY) receptor cDNA was recently cloned from a rat brain library. In situ hybridization histochemistry using a synthetic oligonucleotide probe complimentary to bases 716-764 of the Y1 receptor cDNA was used to localize the corresponding mRNA in the rat brain with special reference to the hypothalamic arcuate-paraventricular axis, because these areas are considered to play a key role in eating behavior. Quantification of the hybridization signals by a computerized image analyzing system revealed that the arcuate nucleus together with the granular cell layer in the gyrus dentatus contained a high concentration of Y1 mRNA. In emulsion-dipped sections, the neurons containing Y1 mRNA transcripts were found particularly in the ventromedial part of the arcuate nucleus. These data indicate that NPY or NPY-related peptides via a Y1 receptor on neurons of the arcuate nucleus may influence the homeostasis.
Mikkelsen
J D
JD
Department B, University of Copenhagen, Denmark.
Larsen
P J
PJ
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000502:physiology*; D001345:Autoradiography; D015723:Gene Library; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D008297:Male; D008473:Median Eminence / Q000166:cytology / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis* / Q000235:genetics; D013462:Sulfur Radioisotopes; D014158:Transcription, Genetic
D012333:RNA, Messenger; D017476:Receptors, Neuropeptide Y; D013462:Sulfur Radioisotopes