Effect of haemodialysate and its peptide fractions on lactate dehydrogenase activity in erythrocytes from healthy subjects and patients with end-stage renal disease.
The effect of haemodialysate and its 3 peptide fractions on lactate dehydrogenase activity in erythrocytes was studied in patients treated by repeated haemodialysis for end-stage renal disease and in healthy subjects. Erythrocytes from dialyzed patients showed significantly lower LDH activity than those from healthy subjects. After a 3-h incubation with haemodialysate (675 and 450 micrograms protein/ml), or its peptide fraction III (270 and 190 micrograms protein/ml), a significant inhibition of LDH activity was observed. On the other hand, neither haemodialysate nor its peptide fractions inhibited LDH activity in red blood cells from patients with end-stage renal disease treated by repeated haemodialysis.
Smoleński
O
O
Department of Nephrology, Medical Academy, Cracow, Poland.
Tabarowski
Z
Z
Miszta
H
H
Dabrowski
Z
Z
D001798:Blood Proteins / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D007676:Kidney Failure, Chronic / Q000097:blood / Q000201:enzymology* / Q000628:therapy; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism*; D006435:Renal Dialysis
D001798:Blood Proteins; D007770:L-Lactate Dehydrogenase