Coxsackievirus B3 from an infectious cDNA copy of the genome is cardiovirulent in mice.
A coxsackievirus B3 (CVB3) cDNA clone, upon transfection of HeLa cells, produces CVB3 capable of induction of cardiac inflammation in C3H/He mice by day 8 post inoculation (p.i.). Liver and serum are cleared of CVB3 by day 8 p.i., but CVB3 persists in the heart through day 14. The nucleotide sequence and the predicted amino acid sequence of this clone have sites of divergence from 2 other completely sequenced CVB3 genomes although overall identity of the three CVB3 genomes is 99%.
Tracy
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Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha.
Chapman
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D000818:Animals; D003384:Coxsackievirus Infections / Q000382:microbiology*; D004279:DNA, Viral; D029822:Enterovirus B, Human / Q000235:genetics / Q000472:pathogenicity*; D016679:Genome, Viral; D006367:HeLa Cells; D006331:Heart Diseases / Q000382:microbiology*; D006801:Humans; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D014774:Virulence / Q000235:genetics
D004279:DNA, Viral