Stage-specific binding of inhibin and activin to subpopulations of rat germ cells.
Flow cytometry was used to separate and identify Sertoli and germ cell populations in primary rat testicular cultures derived from animals of different ages on the basis of cell size and DNA and lipid content. Multiparameter fluorescent evaluation of each cell preparation resulted in the assignment of specific staining patterns to Sertoli cells (diploid, high lipid content), spermatogonia (diploid, low lipid content), spermatocytes (large, tetraploid, high lipid content), and round spermatids (haploid, low lipid content). Each field was separately analyzed for inhibin and activin binding. Fluorescein isothiocyanate-conjugated activin bound with greatest intensity to spermatogonia, with little binding to leptotene or zygotene spermatocytes. Fluorescein isothiocyanate-conjugated inhibin bound to all stages of germ cells tested. Cross-competition data indicate that at least two and probably three distinct receptors exist for these peptides.
Woodruff
T K
TK
Department of Cell Culture Research, Genentech, Inc., South San Francisco, California 94080.
Borree
J
J
Attie
K M
KM
Cox
E T
ET
Rice
G C
GC
Mather
J P
JP
D029404:Activin Receptors; D028341:Activins; D000375:Aging; D000818:Animals; D002450:Cell Communication; D002469:Cell Separation; D002478:Cells, Cultured; D005434:Flow Cytometry; D007265:Inhibins / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018000:Receptors, Peptide; D011994:Recombinant Proteins / Q000378:metabolism; D012708:Sertoli Cells / Q000378:metabolism*; D012741:Sexual Maturation; D013090:Spermatocytes / Q000378:metabolism; D013093:Spermatogonia / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*
D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; D011994:Recombinant Proteins; D028341:Activins; D007265:Inhibins; D029404:Activin Receptors