title: Morphine determination by gas chromatography/mass spectroscopy in human vitreous humor and comparison with radioimmunoassay.
abstract: A gas chromatographic-mass spectroscopic method (GC/MS) for morphine determination in human vitreous humor was developed. Reversed-phase solid-liquid extraction gave better sample purification compared with liquid-liquid or normal-phase solid-liquid extractions. In 10 real cases of death by heroin overdose, the correlation between the results obtained by GC/MS and by radioimmunoassay (RIA) was studied.
authors.affiliation: Institute of Legal Medicine, Faculty of Medicine, University of Santiago de Compostela, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: Bermejo
authors.forename: I
authors.initials: I
authors.lastname: Ramos
authors.forename: P
authors.initials: P
authors.lastname: Fernández
authors.forename: M
authors.initials: M
authors.lastname: López-Rivadulla
authors.forename: A
authors.initials: A
authors.lastname: Cruz
authors.forename: M
authors.initials: M
authors.lastname: Chiarotti
authors.forename: N
authors.initials: N
authors.lastname: Fucci
authors.forename: R
authors.initials: R
authors.lastname: Marsilli
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009020:Morphine / Q000032:analysis*; D011863:Radioimmunoassay; D014822:Vitreous Body / Q000737:chemistry*
chemical_list: D009020:Morphine