An ESR-CT imaging of the head of a living rat receiving an administration of a nitroxide radical.
Three-dimensional ESR imaging of a living rat has been performed by an L-band ESR system, which is composed of an L-band ESR spectrometer, a field gradient coil, and a data processor. The imaging was carried out by Lauterbur's method. A nitroxide, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (Carbamoyl-PROXYL), was used as an imaging agent in saline solution at a concentration of 0.2 M and administered intraperitoneally to obtain a constant concentration in the head for about an hour. It took about 40 min to obtain one set of ESR-CT images. The cross-sectional images were made, both as coronal and horizontal images. In the images of the rat head the nitroxide-rich region was clearly distinguished from the deficient region. The nitroxide-deficient areas corresponded well to the brain of the rat.
Ishida
S
S
Department of Neuropsychiatry, Fukushima Medical College, Japan.
Matsumoto
S
S
Yokoyama
H
H
Mori
N
N
Kumashiro
H
H
Tsuchihashi
N
N
Ogata
T
T
Yamada
M
M
Ono
M
M
Kitajima
T
T
D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D003497:Cyclic N-Oxides; D003952:Diagnostic Imaging / Q000379:methods*; D004578:Electron Spin Resonance Spectroscopy; D007091:Image Processing, Computer-Assisted; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013113:Spin Labels
D003497:Cyclic N-Oxides; D013113:Spin Labels; C047253:3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl