title: Immunostaining of formalin-fixed, paraffin-embedded tissues for malignant lymphomas.
abstract: With the advent of new monoclonal antibodies that are applicable to formalin-fixed, paraffin embedded sections, immunophenotyping is becoming increasingly important in the diagnosis and classification of lymphomas. However, multiple factors such as fixation, trypsinization and even type of antibodies used have certain effects on the final outcome of the staining procedure. In this paper we report our experience and the problems encountered in our laboratory when we first tried to establish a workable immunostaining protocol for formalin-fixed, paraffin embedded tissue sections using the immunoalkaline phosphatase technique.
authors.affiliation: Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia.
authors.forename: Y C
authors.initials: YC
authors.lastname: Lim
authors.forename: K S
authors.initials: KS
authors.lastname: Phang
authors.forename: S K
authors.initials: SK
authors.lastname: Cheong
mesh_terms: D000469:Alkaline Phosphatase; D000911:Antibodies, Monoclonal; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D008223:Lymphoma / Q000473:pathology*; D016612:Paraffin Embedding; D016707:Tissue Fixation / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D005557:Formaldehyde; D000469:Alkaline Phosphatase