In vitro influence of oxygenation on lipogenesis of rat adipose tissue.
Pre-incubation of rat adipose tissue from ad lib fed rats in gas mixtures containing a range of oxygen concentrations from 0% oxygen to 20% oxygen showed that incorporation of isotopes of glucose into carbon dioxide and tissue were increased after pre-incubation for a period of 14-16 hours in a gas atmosphere containing 5% oxygen. This increase required the presence of 1.2 U/ml insulin in the pre-incubation medium and it was augmented by the presence of 2.8 x 10(-7) M prostaglandin F2 alpha. Incorporation into tissue was shown to be mainly into glyceride-glycerol. Similar results were obtained with isolated fat cells.
Macfarlane
C M
CM
Department of Chemical Pathology, Faculty of Medicine, University of Stellenbosch, Tygerberg Hospital, Republic of South Africa.
D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D015237:Dinoprost / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D007328:Insulin; D008055:Lipids; D002245:Carbon Dioxide; D015237:Dinoprost; D005947:Glucose; D010100:Oxygen