T-cell receptor beta usage by 35 different antigen-specific T-cell clones restricted by HLA-Dw4 or -Dw14.1.
We studied whether antigen-specific T cells being restricted by the very similar HLA-Dw4 and/or -Dw14.1 molecules might demonstrate homogeneities in parts of their TCR. TCCs were generated from three individuals who were all HLA-Dw4/Dw14.1 heterozygous. Thirty-five TCCs specific for PPD or TT and restricted by HLA-Dw4 and/or -Dw14.1 were selected for TCR beta gene sequencing. We found that 19 different V beta genes from 13 V beta families were expressed by these TCCs. Thus, it seems that many different TCRV beta genes may be used by TCCs restricted by these HLA molecules. For PPD-specific TCCs, a possible biased usage of V beta 8, as well as possible preferential usage of a CDR3 motif, were found.
Hansen
T
T
Institute of Transplantation Immunology, The National Hospital, Oslo, Norway.
Qvigstad
E
E
Lundin
K E
KE
Thorsby
E
E
D000595:Amino Acid Sequence; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001483:Base Sequence; D002999:Clone Cells; D000939:Epitopes / Q000276:immunology; D005260:Female; D006681:HLA-D Antigens / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D006681:HLA-D Antigens; C080038:HLA-DW14.1 antigen; C053495:HLA-Dw4 antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta