[Bacterial microcolony and its primary applications].
A new rapid and useful microcolony technique was developed by using cellulose-acetate membrane as a supporter. The bacteria were inoculated on the membrane and cultivated for 3-6h. Then the membrane was rendered, fixed and colored. The microcolonies were observed and counted under the microscope. By using this technique the microcolonies of 139 strains of bacteria were observed. 72 strains of Staphylococcus were differentiated by means of observing the characteristics of the microcolonies. The correlation rate between the microcolony method and the routine method was 90.28%. This technique was also first used to determine the bacteria numbers in urine within 6h, and the advantages of it were significant compared with the plate count method.
Pei
X
X
Ye
M
M
Yin
Q
Q
Zhang
C
C
Xu
X
X
D015169:Colony Count, Microbial / Q000379:methods*; D004755:Enterobacteriaceae / Q000254:growth & development; D013210:Staphylococcus / Q000254:growth & development; D013291:Streptococcus / Q000254:growth & development