title: [Combined action of doxycycline and mytilan on the immune response to tularemia antigen].
abstract: Combined action of doxycycline and mytilan, a natural polysaccharide, on the primary immune response to the antigen of the tularemia vaccinal strain in CBA mice was studied. The polysaccharide was used to compensate the immunosuppressive effect of doxycycline high doses on the humoral immune response. The maximum stimulation of the antibody titers as compared to the controls (more than 250 per cent) was observed when mytilan was administered simultaneously with or prophylactically 3 days prior to the antibiotic in doses of 2.5 and 25 mg/kg. The use of mytilan in combination with doxycycline high doses made it possible to compensate the antibiotic-induced decrease of DTH and even to stimulate it as compared to the controls. The highest levels of DTH (150 per cent against the control) were observed when mytilan was administered prophylactically in doses of 2.5 and 11.25 mg/kg 3 days prior to immunization. Mytilan had the highest stimulating effect on antibody production. The combined use of doxycycline and mytilan was characterized by significant stimulation of antibody production and DTH when the dose/time regimens were rational.
authors.forename: V E
authors.initials: VE
authors.lastname: Zakharov
authors.forename: N N
authors.initials: NN
authors.lastname: Besednova
authors.forename: N V
authors.initials: NV
authors.lastname: Krylova
authors.forename: A V
authors.initials: AV
authors.lastname: Nikitin
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000187:drug effects*; D000998:Antiviral Agents / Q000627:therapeutic use; D004318:Doxycycline / Q000627:therapeutic use; D004359:Drug Therapy, Combination / Q000627:therapeutic use*; D006968:Hypersensitivity, Delayed / Q000188:drug therapy; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011134:Polysaccharides / Q000627:therapeutic use; D014406:Tularemia / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000998:Antiviral Agents; D011134:Polysaccharides; C030207:mytilan; D004318:Doxycycline
vernacular_title: Kombinirovannoe deĭstvie doksitsiklina i mitilana na immunnyĭ otvet k tuliaremiĭnomu antigenu.