title: Characterization of lactoferrin binding by Aeromonas hydrophila.
abstract: Various lactoferrin preparations (iron-saturated and iron-depleted human milk lactoferrins and bovine milk and colostrum lactoferrins) were bound by Aeromonas hydrophila. Binding was (i) reversible (65% of bound lactoferrin was displaced by unlabeled lactoferrin), (ii) specific (lactoferrin but not other iron-containing glycoproteins such as ferritin, transferrin, hemoglobin, and myoglobin inhibited binding), and (iii) significantly reduced by pepsin and neuraminidase treatment of the bacteria. The glycosidic domains of the lactoferrin molecule seem to be involved in binding since precursor monosaccharides of the lactoferrin oligosaccharides (mannose, fucose, and galactose) and glycoproteins which have homologous glycosidic moieties similar to those of the lactoferrin oligosaccharides (asialofetuin or fetuin) strongly inhibited lactoferrin binding. A. hydrophila also binds transferrin, ferritin, cytochrome c, hemin, and Congo red. However, binding of these iron-containing compounds seems to involve bacterial surface components different from those required for lactoferrin binding. Expression of lactoferrin binding by A. hydrophila was influenced by culture conditions. In addition, there was an inverse relationship between lactoferrin binding and siderophore production by the bacterium.
authors.affiliation: Department of Medical Microbiology, University of Lund, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Ascencio
authors.forename: A
authors.initials: A
authors.lastname: Ljungh
authors.forename: T
authors.initials: T
authors.lastname: Wadstrom
mesh_terms: D016980:Aeromonas hydrophila / Q000254:growth & development / Q000378:metabolism*; D003224:Congo Red / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D003470:Culture Media; D003574:Cytochrome c Group / Q000378:metabolism; D005293:Ferritins / Q000378:metabolism; D006427:Hemin / Q000378:metabolism; D007700:Kinetics; D007781:Lactoferrin / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism
chemical_list: D003470:Culture Media; D003574:Cytochrome c Group; D014168:Transferrin; D003224:Congo Red; D006427:Hemin; D003300:Copper; D005293:Ferritins; D007781:Lactoferrin