title: Staphylococcal lipase affects phagocytosis of Staphylococcus aureus by human granulocytes and monocytes.
abstract: The rate of immunological and non-immunological phagocytosis of staphylococci by lipase pre-treated human granulocytes and monocytes was compared. It was found that the effect of this enzyme on two types of cells is opposite. Lipase decreases phagocytosis by granulocytes and increases by monocytes. The revealed differences between phagocytosing cells studied prompted us to investigate the influence of lipase on Fc receptors on these cells (rosette EA test). The different susceptibility of Fc receptors on non-activated phagocytes to lipase was found. This could be at least partially responsible for the difference observed between phagocytic activity of granulocytes (decreased) and monocytes (increased) pretreated with staphylococcal lipase. Inactivated enzyme showed a similar effect as active enzyme in the case of granulocytes. However, inactivated enzyme had no effect on rosette formation by lipase pretreated monocytes, indicating an enzymatic effect.
authors.affiliation: Institute of Zoology, Warsaw University, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Izdebska-Szymona
authors.forename: S
authors.initials: S
authors.lastname: Tyski
authors.forename: N
authors.initials: N
authors.lastname: Drela
authors.forename: E
authors.initials: E
authors.lastname: Sobiczewska
authors.forename: M
authors.initials: M
authors.lastname: Laskowska
authors.forename: D
authors.initials: D
authors.lastname: Kalkhof
authors.forename: W
authors.initials: W
authors.lastname: Hryniewicz
mesh_terms: D004306:Dose-Response Relationship, Immunologic; D006098:Granulocytes / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D008049:Lipase / Q000494:pharmacology*; D009000:Monocytes / Q000276:immunology / Q000382:microbiology*; D010587:Phagocytosis / Q000187:drug effects*; D011961:Receptors, Fc / Q000187:drug effects; D012397:Rosette Formation; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000276:immunology*
chemical_list: D011961:Receptors, Fc; D008049:Lipase