An ocular model of adenovirus type 5 infection in the NZ rabbit.
Ocular adenoviral infections occur worldwide, and currently, there is no ocular animal model for evaluating new antivirals or studying pathogenesis. With a paired-eye design, an ocular model was developed in 32 New Zealand rabbits following topical and intrastromal inoculation with a clinical isolate of adenovirus type 5 (Ad5 McEwen). Clinical signs of infection--conjunctivitis, corneal edema, subepithelial infiltrates, and iritis--and seroconversion were evaluated. Replicating virus on the ocular surface was determined by serial ocular titers. Reproducible acute ocular infection was demonstrated in 32 of 32 infected eyes (100%), with mean viral replication lasting for 8.3 days. Peak ocular viral titers (10(3) plaque forming units/ml) were achieved on day three after inoculation and represented a 2 log increase (100 times) over day one. Ocular viral replication was associated with acute conjunctivitis (24/34 eyes, 75%), and delayed-onset presumed immune-mediated clinical disease was associated with: blepharoconjunctivitis (21/32 eyes, 66%), iritis (29/32 eyes, 91%), corneal edema (32/32 eyes, 100%), and subepithelial corneal infiltrates (30/32 eyes, 94%). Seroconversion was demonstrated in 26 of 31 rabbits (84%). The study concludes that a potentially useful animal model of adenoviral ocular infection can be attained.
Gordon
Y J
YJ
Department of Ophthalmology, University of Pittsburgh School of Medicine, Pennsylvania.
Romanowski
E
E
Araullo-Cruz
T
T
D000257:Adenoviridae Infections / Q000382:microbiology* / Q000473:pathology; D000260:Adenoviruses, Human; D000818:Animals; D003231:Conjunctivitis / Q000382:microbiology; D003316:Corneal Diseases / Q000382:microbiology* / Q000473:pathology; D015715:Corneal Edema / Q000382:microbiology; D003319:Corneal Stroma / Q000382:microbiology / Q000473:pathology; D004195:Disease Models, Animal; D015828:Eye Infections, Viral / Q000382:microbiology* / Q000473:pathology; D005260:Female; D007500:Iritis / Q000382:microbiology; D011817:Rabbits; D010948:Viral Plaque Assay; D014779:Virus Replication