Physical linkage of two mammalian imprinted genes, H19 and insulin-like growth factor 2.
Parental imprinting is a phenomenon in mammals whereby the maternal and paternal alleles of a gene are differentially expressed. Three murine genes have been shown to display this type of allele-specific expression. Two of them, insulin-like growth factor-2 (Igf-2) and H19, map to the distal end of mouse chromosome 7, but are imprinted in opposite directions. Pulsed-field gel electrophoresis and large-fragment DNA cloning were utilized to establish a physical map that includes H19 and Igf-2. Igf-2 lies approximately 90 kilobases of DNA 5' to H19, in the same transcriptional orientation. This physical proximity is conserved in humans, based on pulsed-field gel analysis. We conclude that H19 and Igf-2 constitute an imprinted domain.
Zemel
S
S
Howard Hughes Medical Institute, Princeton University, New Jersey 08544.
Bartolomei
M S
MS
Tilghman
S M
SM
D000818:Animals; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D008040:Genetic Linkage; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008297:Male; D051379:Mice; D015183:Restriction Mapping; D012727:Sex Characteristics
D007335:Insulin-Like Growth Factor II