Detection of human papillomavirus DNA sequences in tongue squamous-cell carcinoma utilizing the polymerase chain reaction method.
Twenty-four cases of tongue squamous-cell carcinoma (SCC) were analyzed for human papillomavirus (HPV) DNAs by the polymerase chain reaction (PCR) method and the dot-blot hybridization technique. HPV DNAs were detected in 8 cases. One specimen histopathologically diagnosed as poorly differentiated grade-III SCC contained both HPV-16 and HPV-18 DNA, and 7 other cases contained HPV-16 DNA.
Shindoh
M
M
Department of Molecular Biology, Sapporo Medical College, Japan.
Sawada
Y
Y
Kohgo
T
T
Amemiya
A
A
Fujinaga
K
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data*; D014062:Tongue Neoplasms / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D014412:Tumor Virus Infections / Q000150:complications / Q000235:genetics*
D004279:DNA, Viral