title: Automated sequential affinity chromatography of sea urchin embryo DNA binding proteins.
abstract: An automated method of running a tandem sequence of oligonucleotide affinity columns was used to purify factors that interact specifically with cis-regulatory sites of the CyIIIa cytoskeletal actin gene of the sea urchin embryo (Strongylocentrotus purpuratus). The method allows quantitative enrichment in a single chromatographic run of up to 12 different sequence-specific DNA binding proteins, each of which may then be readily purified to homogeneity by methods such as preparative gel electrophoresis. The affinity chromatography and identification of six different CyIIIa-regulatory factors is described, and the general utility of the method is discussed.
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena 91125.
authors.forename: J A
authors.initials: JA
authors.lastname: Coffman
authors.forename: J G
authors.initials: JG
authors.lastname: Moore
authors.forename: F J
authors.initials: FJ
authors.lastname: Calzone
authors.forename: R J
authors.initials: RJ
authors.lastname: Britten
authors.forename: L E
authors.initials: LE
authors.lastname: Hood
authors.forename: E H
authors.initials: EH
authors.lastname: Davidson
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002846:Chromatography, Affinity; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides; D012617:Sea Urchins / Q000196:embryology
chemical_list: D000199:Actins; D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides