Neuropeptide Y and catecholamine release in the piglet during hypoxia: enhancement by theophylline.
Sympathoadrenal activity was studied in 13 young piglets during hypoxia. The piglets were anaesthetized with chloralose/urethane, tracheostomized, paralyzed with gallamine and artificially ventilated. A femoral artery catheter was inserted and used for blood sampling. The piglets were challenged with 6 min of 6% CO2, 10 min of 12% O2 and 6 min of 6% O2 before and after theophylline (an adenosine receptor antagonist) treatment 20 mg/kg (n = 9) or saline (n = 4). Plasma samples were obtained before, during and after each hypercapnic or hypoxic period and analysed for their content of noradrenaline, adrenaline and neuropeptide Y. Hypercapnia with 6% CO2 and moderate hypoxia with 12% O2 did not lead to any significant increase of either noradrenaline (NA), adrenaline (A) or neuropeptide Y (NPY). However, severe hypoxia with 6% O2 increased the NA level from 30 to 66 nmol/l; the A level from 1 to 28 nmol/l and NPY from 140 to 213 pmol/l. After treatment with theophylline the baseline NA increased from 27 to 40 nmol/l, A rom 1.5 to 4.0 and NPY concentration from 65 to 171 pmol/l. Theophylline moderately enhanced the release of NPY, NA and A during the 12% O2 challenge. However, during the severe hypoxia (6%), the increase of NA (from 49 to 333 nmol/l), A (from 8 to 214 nmol/l) and NPY (from 184 to 385 pmol/l) showed considerably enhancement after the theophylline treatment. The results obtained before and after saline were similar showing that the duration of the experiments per se did not change the baseline levels or the effect of the challenges on NA, A or NPY levels.(ABSTRACT TRUNCATED AT 250 WORDS)
Thoresen
M
M
Department of Neurophysiology, Karolinska Institute, Stockholm, Sweden.
Dahlin
I
I
Lundberg
J M
JM
Lagercrantz
H
H
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002245:Carbon Dioxide / Q000097:blood; D004837:Epinephrine / Q000097:blood*; D000860:Hypoxia / Q000097:blood*; D009478:Neuropeptide Y / Q000097:blood*; D009638:Norepinephrine / Q000097:blood*; D010100:Oxygen / Q000097:blood; D013552:Swine; D013806:Theophylline / Q000494:pharmacology*
D009478:Neuropeptide Y; D002245:Carbon Dioxide; D013806:Theophylline; D010100:Oxygen; D009638:Norepinephrine; D004837:Epinephrine