title: [Study on the meiotic mapping and functions of another new osmotic-sensitive gene-osm3 in Saccharomyces cerevisiae].
abstract: Another recessive nuclear gene mutation of Sacchromyces cerevisiae, osm3, which causes growth inhibition on hypertonic media, has been mapped on the chromosome II by tetrad analysis. Its second-division segregation frequency is about 51.01%, and the map distances between osm3 and the centromere of chromosome II is about 25.51 centimorgans and it is approximately 45 centimorgans far gene gall. The studies on the reverse mutation of osm3 indicated that this osmotic-sensitivity arises from a missense or nonsense mutation in OSM3 locus. The response of yeast to osmotic stress and the mechanism of osm3 gene function were investigated. The results indicated that high intracellular glycerol content is necessary for yeast to grow in hypertonic media; The gene product of osm3 is probably relative to the process of glycerol transportation. Finally, the effect of osmotic stress on fermentability was also discussed.
authors.affiliation: Department of Biology, Hangzhou University.
authors.forename: H
authors.initials: H
authors.lastname: Zhi
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Chen
mesh_terms: D002874:Chromosome Mapping; D005800:Genes, Fungal / Q000502:physiology; D005990:Glycerol / Q000378:metabolism; D008540:Meiosis; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D005990:Glycerol