Inhibitory effect of myristylation on transrepression by FBR (Gag-Fos) protein.
The myristylated v-fos product, FBR murine sarcoma virus (Gag-Fos) protein, exhibits a lower level of transrepression of the serum response element (SRE) than does c-fos protein (Fos). Mutation of the N-terminal myristylation site in FBR protein restored SRE transrepression. Replacement of N-terminal viral Gag sequences with the Fos N terminus also restored this activity, providing additional evidence that myristylation inhibits transrepression by FBR protein. However, the myristylated Gag domain did not inhibit SRE transrepression when fused to Fos, indicating that myristylation of a fos protein is not by itself sufficient to prevent SRE transrepression and that C-terminal mutation is necessary to inhibit transrepression by N myristylation. Comparison of transfection results with Fos C-terminal deletion mutants and the Fos/FBR chimeric mutant revealed that the FBR C terminus retained the potential for transrepression despite deletion of the normal Fos C terminus, whereas similar Fos deletion mutants did not. These results indicate that both N- and C-terminal mutations are required to inhibit transrepression by FBR protein and that multiple structural mutations accompanied by posttranslational protein modification alter gene regulation by FBR protein.
Kamata
N
N
Department of Cell Biology, Vanderbilt University, Nashville, Tennessee 37232.
Holt
J T
JT
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015681:Fusion Proteins, gag-onc / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D016761:Oncogene Proteins v-fos / Q000235:genetics*; D011233:Precipitin Tests; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D009053:Sarcoma Viruses, Murine / Q000235:genetics*
D015681:Fusion Proteins, gag-onc; D009227:Myristic Acids; D016761:Oncogene Proteins v-fos; D016760:Proto-Oncogene Proteins c-fos; C067019:gag-fos protein, FBR murine sarcoma virus; D019814:Myristic Acid