Evidence for heat-stable liver cytosol substance(s) capable of causing oxidative activation of fructose 1,6-bisphosphatase.
The endogenous fructose 1,6-bisphosphatase (FBPase) in chicken liver extract undergoes a drastic increase in activity if the pH of the extract is in the alkaline range. Greater and more consistent activation occurs when purified FBPase, placed inside dialysis sack, is incubated in liver extract. Maximal activation (over 16-fold) is accompanied by the disappearance of 4 highly reactive sulfhydryl groups (SH) per molecule of enzyme. The activating effect of the extract remains essentially unchanged after heating to 100 degrees C. Activation can be reversed by dithiothreitol. These data show the existence in liver cytosol of heat-stable substance(s) capable of activating FBPase presumably by forming disulfide bonds with the enzyme's highly reactive SH groups.
Han
G
G
Department of Chemistry, Morehouse College, Atlanta, Georgia 30314.
Mack
D
D
Hang
J
J
Hunter
E
E
Zeidan
H
H
Han
P
P
D000818:Animals; D002645:Chickens; D003600:Cytosol / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D004355:Drug Stability; D004789:Enzyme Activation; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*
D011506:Proteins; D006597:Fructose-Bisphosphatase; D004229:Dithiothreitol