Investigation of the solid- and solution-phase binding reactivities of continuous epitopes recognized by polyclonal guinea-pig anti-recombinant bovine growth hormone antisera.
We have used the technique of multiple pin peptide synthesis to identify three major continuous epitopes in the recombinant bovine (rb) GH molecule. We have synthesized these peptides, residues 24-40, 139-152 and 179-189, as N-terminally acetylated, C-terminal amides and confirmed their reactivity in a standard solid-phase ELISA. Subsequently, for epitope 139-152, we have synthesized a peptide affinity column and used this to isolate antibodies with this epitope specificity from whole antiserum. In addition, we demonstrate that under native conditions in a liquid phase RIA, these antibodies will precipitate [125I]rbGH. Further, peptide 139-152 itself also cross-reacts in an rbGH RIA inhibiting binding by up to 20%. Our data suggest that during the immune response to rbGH in guinea-pigs a substantial part of the B-cell response is directed to the 139-152 region and that this part of the protein is a native epitope.
Beattie
J
J
Hannah Research Institute, Ayr, UK.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D000939:Epitopes / Q000276:immunology*; D005260:Female; D013006:Growth Hormone / Q000276:immunology*; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D012996:Solutions
D000939:Epitopes; D007106:Immune Sera; D010446:Peptide Fragments; D011994:Recombinant Proteins; D012996:Solutions; D013006:Growth Hormone