title: Cloning and sequencing of human kappa-casein cDNA.
abstract: A cDNA encoding kappa-casein of human milk was cloned and sequenced. The kappa-casein cDNA was isolated from a lambda gt11 library generated from mRNA prepared from a mammary gland biopsy obtained from a lactating woman. The library was screened with polyclonal rabbit antibodies raised against purified native kappa-casein. The obtained nucleotide sequence contained an ORF sufficient to encode the entire amino acid sequence of a kappa-casein precursor protein consisting of 182 amino acids. This includes a tentative signal peptide of 20 amino acids and a processed protein of 162 amino acids.
authors.affiliation: Department of Microbiology, University of Umeå, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Bergström
authors.forename: L
authors.initials: L
authors.lastname: Hansson
authors.forename: O
authors.initials: O
authors.lastname: Hernell
authors.forename: B
authors.initials: B
authors.lastname: Lönnerdal
authors.forename: A K
authors.initials: AK
authors.lastname: Nilsson
authors.forename: M
authors.initials: M
authors.lastname: Strömqvist
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D002364:Caseins; D012333:RNA, Messenger; D004247:DNA