Gestational modulation of myometrial proteins in the timed-pregnant Sprague-Dawley rat.
These studies sought to test the hypothesis that the expression of myometrial proteins is modulated as the onset of parturition approaches. Myometrial proteins from timed-pregnant rats were analyzed utilizing sodium dodecylsulfate polyacrylamide gel and 2-dimensional electrophoresis, and Western blot techniques. SDS-PAGE gels demonstrated increased expression of at least 10 protein bands from 17 to 200+ KD. 2-dimensional gels confirmed the presence of at least five groups of gestationally modulated proteins. Western blots for phosphoinositide-specific phospholipase C demonstrated significant modulation of the expression of three isozymes. These studies have confirmed differential expression of myometrial proteins near term in the timed-pregnant rat; some of which play an important role in intracellular signal transduction in response to hormones and pharmacologic agents.
Phillippe
M
M
Department of Obstetrics and Gynecology, Pritzker School of Medicine, University of Chicago.
Harrison
H H
HH
D000818:Animals; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005865:Gestational Age; D007526:Isoelectric Point; D008970:Molecular Weight; D009215:Myometrium / Q000378:metabolism*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D011506:Proteins; D010727:Phosphoric Diester Hydrolases; D043265:Phosphatidylinositol Diacylglycerol-Lyase