[Filtration of buffy coat-free erythrocyte suspensions in additive solution].
Buffy coat-free red cell concentrates in SAG-M (RCC) were produced by BAT system (leukocyte content 132 x 10(6)/RCC). They were stored for 4 weeks and filtered by Erypur Optima (E), Sepacell R 500 B (S) und PALL RC 50 TM (P). Leukocyte depletion was very effective (E: 0.56 x 10(6)/RCC; S: 0.36 x 10(6)/RCC; P: 0.55 x 10(6)/RCC) but hemolysis was remarkable (E: 301 +/- 195; S: 127 +/- 123; P: 368 +/- 256 mg/RCC). Therefore preparation of two RCC per filter was only acceptable with S. In S and P loss of red cells was tolerable (E: 90.5; S. 41.8; P: 38.2). In contrast, E should be rinsed with sodium chloride at the end of the preparation. In E and S filtration times were short without additional pressure (E: 6.6 min; S: 4.7 min; P: 20.3 min). We conclude from our results that the use of buffy coat-free red cell concentrates in additive solution considerably reduces the problems of filtration, e.g. storage interval, leukocyte reduction, hemolysis, filtration flow. Despite this, bedside filtration is not recommended because quality assurance is necessary.
Dietrich
K
K
Klinikum Marburg, Abteilung Transfusionsmedizin und Gerinnungsphysiologie, BRD.
Dietrich
G
G
Kretschmer
V
V
Eckle
R
R
Heins
M
M
D016913:Blood Component Transfusion / Q000295:instrumentation*; D006452:Hemoglobinometry; D006461:Hemolysis / Q000276:immunology; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation*; D007958:Leukocyte Count; D008212:Lymphocyte Depletion / Q000295:instrumentation*; D010976:Platelet Count; D014462:Ultrafiltration / Q000295:instrumentation*
Filtration buffy-Coat-armer Erythrozytensuspensionen in additiver Lösung.