Simultaneous assessment of TGFB and cell cycle kinetics using IUdR/BrdU infusions in human neoplasms from plastic-embedded tissue.
We describe an immunohistochemical technique that makes use of two monoclonal antibodies (MAb), one to detect the transforming growth factor B (TGFB) and another that reacts with iodo- and bromodeoxyuridine. The purpose of this technique is to determine the relationship between TGFB expression and the S-phase cells in human tumors. Since both can be distinctly identified in situ from tissue embedded in plastic, in assessment of the geographic orientation of S-phase cells in relation to such factors as TGFB, contiguity to blood vessels, nerve fibers, and macrophages can also be achieved.
Miller
M A
MA
Department of Internal Medicine, University of Cincinnati Medical Center, Ohio.
Bokhari
S A
SA
Qadir
K
K
Raza
A
A
D000911:Antibodies, Monoclonal; D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology; D001973:Bromodeoxyuridine; D002453:Cell Cycle; D003110:Colonic Neoplasms / Q000235:genetics; D005260:Female; D005909:Glioblastoma / Q000235:genetics; D006652:Histological Techniques; D006801:Humans; D007065:Idoxuridine; D007202:Indicators and Reagents; D007700:Kinetics; D009369:Neoplasms / Q000473:pathology*; D016196:S Phase; D016212:Transforming Growth Factor beta / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D016212:Transforming Growth Factor beta; D001973:Bromodeoxyuridine; D007065:Idoxuridine