Interleukin-2 production by primary adult T cell leukemia tumor cells is macrophage dependent.
We have investigated the cellular requirements for IL-2 production by autocrine proliferating tumor cells from four patients with adult T cell leukemia (ATL). Cultures of these ATL cells both produced endogenous IL-2 protein in the absence of added mitogen and proliferated at higher levels when exogenous recombinant IL-2 was added. Depletion of macrophages in the tumor cell cultures resulted in a sharp decline in tumor cell IL-2 production, while re-addition of macrophages reconstituted this response. Macrophage-derived factors including IL-6 and IL-1 also reconstituted IL-2 production in these macrophage depleted cultures. These results raise the possibility that macrophages may play a central role in HTLV-I mediated immortalization of T cells.
Arima
N
N
First Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Japan.
Daitoku
Y
Y
Hidaka
S
S
Yamamoto
Y
Y
Fujimoto
K
K
Matsushita
K
K
Ohtsubo
H
H
Fukumori
J
J
Tanaka
H
H
Onoue
K
K
D015152:Blotting, Northern; D016922:Cellular Senescence; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D015458:Leukemia, T-Cell / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D008264:Macrophages / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D012016:Reference Values; D013601:T-Lymphocytes / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; D012333:RNA, Messenger; D012334:RNA, Neoplasm