Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice.
The generation of a panel of monoclonal antibodies specific for different variable (V) regions of human T cell receptors will be of great importance in the study of T cell-mediated diseases. However, relatively few such reagents exist, due in part to the poor immunogenicity of TcRs on the surface of human T cells. We have employed a strategy in which T cells from a transgenic mouse line expressing a human V beta 3 C beta 1 TcR were used to immunise syngeneic conventional mice to generate two monoclonal antibodies specific for human T cell receptors. Binding of antibody JOVI.3, which stained approximately 5% of human peripheral blood CD3 positive T cells, correlated with the expression of the human TcR V beta 3 gene segment. Antibody JOVI.1 recognised a determinant on the majority of TcRs, staining 50-75% of peripheral blood T cells and T cell lines expressing different V beta regions. Some TcRs, however, failed to react with this antibody. Both antibodies immunoprecipitated detergent-solubilised TcR molecules and were capable of inducing proliferation of peripheral blood T cells.
Viney
J L
JL
Imperial Cancer Research Fund, London, U.K.
Prosser
H M
HM
Hewitt
C R
CR
Lamb
J R
JR
Owen
M J
MJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D003902:Detergents / Q000494:pharmacology; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D003902:Detergents; D000939:Epitopes; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011994:Recombinant Proteins