Immunofluorescence detection of the vimentin epitope in chromatin structures of cell nuclei and chromosomes.
We describe immunofluorescence detection of the vimentin epitope, recognized by monoclonal antibody VI-01, in chromatin structures of eukaryotic cell nuclei and chromosomes. The approach used is based on increased sensitivity of 5-bromodeoxyuridine (BrdU)-substituted DNA to UV irradiation-induced crosslinking of DNA with proteins in vivo, by which the proteins interacting with chromosomal DNA can be immunovisualized in situ.
Fidlerová
H
H
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Sovová
V
V
Krekule
I
I
Viklický
V
V
Levan
G
G
D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry*; D002875:Chromosomes / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000032:analysis*; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D051379:Mice; D051381:Rats; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis*
D002843:Chromatin; D004268:DNA-Binding Proteins; D000939:Epitopes; D014746:Vimentin