title: The virulence plasmid does not contribute to growth of Salmonella in cultured murine macrophages.
abstract: The virulence plasmid, characteristic of many serovars of Salmonella sp., and specifically its spv genes, promote intracellular growth of the bacteria in the liver and spleen and are essential for the virulence of these Salmonella serovars in the mouse. In an attempt to establish an in vitro model for studying its function, we evaluated its effect on the intracellular growth of the bacteria in macrophages in culture. We used a number of different macrophage-like cell lines (J774-A.1, IC-21 and PU5-1.8), as well as peritoneal or splenic macrophages from genetically Salmonella-sensitive (Itys, BALB/c) or resistant (Ityr, C3H/HeN) mice, and at different states of activation, stimulated in vivo or in vitro with lipopolysaccharide and/or recombinant gamma interferon. These were found to differ in their ability to suppress or sustain intracellular growth of several Salmonella serovars, but in all cases the growth was independent of the spv genes.
authors.affiliation: National Public Health Institute, Molecular Biology Program, Helsinki, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Riikonen
authors.forename: P H
authors.initials: PH
authors.lastname: Mäkelä
authors.forename: H
authors.initials: H
authors.lastname: Saarilahti
authors.forename: S
authors.initials: S
authors.lastname: Sukupolvi
authors.forename: S
authors.initials: S
authors.lastname: Taira
authors.forename: M
authors.initials: M
authors.lastname: Rhen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D020022:Genetic Predisposition to Disease; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics / Q000382:microbiology; D008809:Mice, Inbred C3H / Q000235:genetics / Q000382:microbiology; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010957:Plasmids / Q000235:genetics*; D011994:Recombinant Proteins; D012475:Salmonella / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
chemical_list: D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D007371:Interferon-gamma