[The effect of cholesterol on membrane potentials, proliferation and migration of cultivated endothelial cells].
The influence of cholesterol added as cholesterol containing liposomes [sphingomyelin/cholesterol (1: 1; mol/mol)] or as cholesterol suspensions on cultured bovine aortic endothelial cells was investigated. 10(-6) mol/l cholesterol enhanced membrane potential, 10(-5) mol/l caused depolarisation. The proliferation of the cells was dependent on the concentration influenced in opposite directions too. The proliferation was stimulated by 10(-6) mol/l cholesterol and inhibited by 10(-5) mol/l. The migratory rate of the cells was increased by 10(-6) mol/l and 10(-5) mol/l cholesterol. Our results suggest that exogenous cholesterol is integrated in membranes of the endothelial cells and causes in this way changes of membrane potential, proliferation rate and migratory activity.
Mende
S
S
Fachrichtung Pharmazie, Ernst-Moritz-Arndt-Universität Greifswald.
Windeck
I
I
Lichtnow
K H
KH
Teuscher
E
E
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002784:Cholesterol / Q000494:pharmacology*; D004727:Endothelium / Q000166:cytology; D008081:Liposomes; D008564:Membrane Potentials / Q000187:drug effects; D013109:Sphingomyelins / Q000494:pharmacology
D008081:Liposomes; D013109:Sphingomyelins; D002784:Cholesterol
Zum Einfluss von Cholesterol auf Membranpotential, Teilung und Migration von kultivierten Endothelzellen.