title: Age-dependent pulmonary clearance of Pseudomonas aeruginosa in a mouse model: diminished migration of polymorphonuclear leukocytes to N-formyl-methionyl-leucyl-phenylalanine.
abstract: Ten-, 20-, and 35-day-old mice were subjected to an aerosol containing Pseudomonas aeruginosa. The lung clearance of the organism was decreased in mice under 20 days of age. This deficiency was accompanied by decreased migration of polymorphonuclear leukocytes (PMN) to the airways in response to the P. aeruginosa challenge. Similar results were obtained in both outbred, C5-sufficient Swiss mice and inbred, C5-deficient DBA/2 mice. The diminished clearance of P. aeruginosa was related to a transient, age-related decrease in PMN chemotaxis to formyl-methionyl oligopeptides. PMN chemotaxis levels similar to those seen in adults were regained by day 35 after birth.
authors.affiliation: Departamentó de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
authors.forename: D O
authors.initials: DO
authors.lastname: Sordelli
authors.forename: M
authors.initials: M
authors.lastname: Djafari
authors.forename: V E
authors.initials: VE
authors.lastname: García
authors.forename: P A
authors.initials: PA
authors.lastname: Fontán
authors.forename: G
authors.initials: G
authors.lastname: Döring
mesh_terms: D000367:Age Factors; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002634:Chemotaxis, Leukocyte; D003114:Colony-Forming Units Assay; D004195:Disease Models, Animal; D007111:Immunity, Cellular; D008168:Lung / Q000201:enzymology / Q000382:microbiology; D051379:Mice; D008811:Mice, Inbred DBA; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000502:physiology*; D009195:Peroxidase / Q000096:biosynthesis; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa
chemical_list: D009240:N-Formylmethionine Leucyl-Phenylalanine; D009195:Peroxidase