Characterization of the human HOX 7 cDNA and identification of polymorphic markers.
cDNA clones for a human HOX 7 gene obtained with homologous clones of Drosophila were used in human gene mapping studies. The human cDNA clone was isolated from a library constructed from human embryonic craniofacial material. The sequence of the cDNA demonstrates significant homology with mouse HOX 7. A search for RFLPs identified MboII and BstEII variants. A CA dinucleotide repeat with 5 alleles was also identified and allowed placement of HOX 7 into a defined linkage map. Evidence for linkage disequilibrium was found with markers tested. These results place the human HOX 7 gene in a defined position on 4p.
Padanilam
B J
BJ
Department of Biology, University of Iowa, Iowa City 52242.
Stadler
H S
HS
Mills
K A
KA
McLeod
L B
LB
Solursh
M
M
Lee
B
B
Ramirez
F
F
Buetow
K H
KH
Murray
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific; D004330:Drosophila / Q000235:genetics; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005260:Female; D015723:Gene Library; D005801:Genes, Homeobox; D014644:Genetic Variation; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D009838:Oligodeoxyribonucleotides; D012313:RNA; D004247:DNA; C024413:endodeoxyribonuclease MboII; D015252:Deoxyribonucleases, Type II Site-Specific; C032823:GGTNACC-specific type II deoxyribonucleases