[Inhibition of elastase activity in human gingival extracts by elastin peptides].
Effects of elastin peptides on elastase activity of gingival biopsy specimen extracts were studied in vitro and in vivo. All the extracts from biopsy specimens from 15 patients with a variety of periodontal diseases demonstrated fairly marked elastase activity. In vitro, addition of elastin peptides produced a mean inhibition of 54% +/- 14% with a concentration of 0.25 mg/ml and a mean inhibition of 90% +/- 6% with 2.5 mg/ml. In vivo treatment of gums with a paste containing 1% elastin peptides reduced elastase activity by 47% in 7 of 8 patients. These data suggest that elastin peptides are effective inhibitors of periodontal elastase activity and may be useful in preparations used for the preventive or curative treatment of periodontal diseases with tissue lysis.
Robert
L
L
Laboratoire de Biologie du Tissu Conjonctif, URA CNRS 1460, Faculté de Médecine, Université Paris XII, Creteil, France.
Tixier
J M
JM
Berenholc
S
S
Levy
O
O
Hornebeck
W
W
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D004549:Elastin / Q000494:pharmacology*; D004791:Enzyme Inhibitors; D005260:Female; D005881:Gingiva / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005883:Gingival Crevicular Fluid / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D010455:Peptides / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D010455:Peptides; D004549:Elastin; D010196:Pancreatic Elastase
Inhibition de l'activité élastasique d'extraits de gencives humaines par les peptides d'élastine.