A simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction.
The use of chelating resin in a simple, rapid and efficient pre-treatment protocol to process formalin-fixed, paraffin-embedded specimens for the polymerase chain reaction (PCR) is described and compared to other pre-treatment techniques. With this modified PCR protocol, a variety of human autopsy and biopsy specimens were investigated for presence of DNA of human papilomaviruses, cytomegalovirus or Epstein-Barr virus. These viruses were detected in a productive or non-/low-productive state. Amplimers generated ranged from 88 to 450 base pairs length. Under the specified technical conditions, a considerable range of DNA molecules can be amplified from paraffin-embedded material, some older than 10 years.
Kiene
P
P
Department of Pathology, University of Hamburg, Federal Republic of Germany.
Milde-Langosch
K
K
Runkel
M
M
Schulz
K
K
Löning
T
T
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology* / Q000473:pathology; D001344:Autopsy; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D006321:Heart / Q000382:microbiology; D006801:Humans; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D008168:Lung / Q000382:microbiology / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology* / Q000473:pathology; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D010920:Placenta / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D014846:Vulvar Neoplasms / Q000382:microbiology* / Q000473:pathology
D004279:DNA, Viral