[Changes in creatine kinase activity and protein spectrum of the developing muscle culture treated with creatine phosphate].
A study was made of the influence of creatine phosphate (CP) on creatine kinase activity and the total protein status of the primary chicken myoblast culture. It has been established that the two-hour exposure of the developing culture in the presence of 2 and 4 mM CP is accompanied by the lowering of the content of water extractable protein and a considerable increase of creatine kinase activity. Meanwhile the total activity of the enzyme rises in the non-differentiated and falls in the differentiated one. Electrophoretic analysis has shown CP to produce not only quantitative but also deep qualitative changes in the myoblast protein spectrum. It is assumed that the changes revealed are nonspecific.
Matiushichev
V B
VB
Sokolov
I N
IN
Lianguzov
A Iu
AIu
Petrova
T A
TA
Goncharova
V I
VI
Petrova
T N
TN
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003402:Creatine Kinase / Q000378:metabolism*; D003470:Culture Media; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000196:embryology / Q000201:enzymology / Q000378:metabolism*; D010725:Phosphocreatine / Q000494:pharmacology*
D003470:Culture Media; D009124:Muscle Proteins; D010725:Phosphocreatine; D003402:Creatine Kinase
Sdvigi kreatinkinaznoĭ aktivnosti i belkovogo spektra razvivaiushcheĭsia myshechnoĭ kul'tury, obrabotannoĭ kreatinfosfatom.