Feeding studies in rats with mineral hydrocarbon food grade white oils.
This investigation compared the effects of feeding rats diets containing food grade white oil processed by either conventional oleum treatment or the more modern method of catalytic hydrogenation. In two separate experiments, male or female Fischer-344 rats were given free access for 90 days to diets containing 0, 10, 100, 500, 5,000, 10,000, or 20,000 ppm of either oleum-treated white oil (OTWO) or hydrotreated white oil (HTWO). There were no mortalities and no adverse clinical signs associated with feeding either white oil. Treatment-related effects evidenced by hematological, clinical chemical, and pathological changes were generally dose-related and more marked in female than in male rats, and the OTWO caused a greater pathological response than the HTWO. Tissue residues of saturated hydrocarbons were up to 5.2 times higher in female rats than in males. Rats fed 5,000 ppm or more of either white oil showed dose-related alterations in several hematological and clinical chemistry variates associated mainly with hepatic damage or functional alteration. At necropsy, mesenteric lymph nodes were enlarged, and increases in weight of liver, kidney, and spleen were significant. Microscopic changes were characterized by multifocal lipogranulomata in mesenteric lymph node and liver. No changes were observed in rats fed OTWO or HTWO for 90 days at dietary concentrations of 10 or 100 ppm, equivalent to a minimum intake of 0.65 and 6.4 mg/kg/day, respectively. Differences in degree of pathological response associated with each oil may have been due to their differences in specification rather than processing method.
Baldwin
M K
MK
Shell Research Ltd., Sittingbourne Research Centre, Kent, United Kingdom.
Berry
P H
PH
Esdaile
D J
DJ
Linnett
S L
SL
Martin
J G
JG
Peristianis
G C
GC
Priston
R A
RA
Simpson
B J
BJ
Smith
J D
JD
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D005260:Female; D006838:Hydrocarbons / Q000493:pharmacokinetics / Q000633:toxicity*; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008297:Male; D008899:Mineral Oil / Q000493:pharmacokinetics / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D012727:Sex Characteristics; D013154:Spleen / Q000473:pathology
D006838:Hydrocarbons; D008899:Mineral Oil