[The marker histochemical detection of glucose-6-phosphate residues in the human placenta].
Glucose-6-phosphate residues were revealed by fine structural analysis using glucose-6-phosphate dehydrogenase-gold conjugate. In human mature placenta specific staining was detected over the stroma of placental villi. Colloidal gold particles were found over the collagen fibrils and reticular lamina of basal membrane. Syncytiotrophoblast cells, fibroblasts, endothelial cells of fetal capillaries avoided labelling. Nucleated blood cells and thrombocytes inside the lumen of fetal capillaries demonstrated intense staining. The present investigation demonstrates histochemically the process of extracellular glycosylation. Glycated collagen fibrils formed channels inside the stroma of placental villi.
Glazyrin
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Kolesnikov
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D015415:Biomarkers; D003094:Collagen / Q000378:metabolism; D019298:Glucose-6-Phosphate; D005954:Glucosephosphate Dehydrogenase; D005958:Glucosephosphates / Q000378:metabolism*; D006046:Gold; D006651:Histocytochemistry; D016591:Histocytological Preparation Techniques; D006801:Humans; D010920:Placenta / Q000378:metabolism* / Q000648:ultrastructure
D015415:Biomarkers; D005958:Glucosephosphates; D019298:Glucose-6-Phosphate; D006046:Gold; D003094:Collagen; D005954:Glucosephosphate Dehydrogenase
Markerno-gistokhimicheskoe vyiavlenie ostatkov gliukozo-6-fosfata v platsente cheloveka.