Human hepatocyte growth factor stimulates the growth of HUH-6 clone 5 human hepatoblastoma cells.
The effects of human hepatocyte growth factor (hHGF), a potent mitogen for rat and human hepatocytes in primary culture, on proliferation of human hepatoma and hepatoblastoma cells were examined. Out of five cell lines; HLE, HuH-6 clone 5, HuH-7, PLC/PRF/5, and Hep G2, only HuH-6 Clone 5 cells were stimulated by recombinant hHGF. Both native and recombinant hHGFs caused dose-dependent increases in cell number and DNA synthesis of cells. This stimulation was strongly inhibited by anti-hHGF monoclonal antibody.
Miyazaki
M
M
Department of Cell Biology, Okayama University Medical School, Japan.
Gohda
E
E
Tsuboi
S
S
Tsubouchi
H
H
Daikuhara
Y
Y
Namba
M
M
Yamamoto
I
I
D000818:Animals; D000911:Antibodies, Monoclonal; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006133:Growth Substances / Q000276:immunology / Q000494:pharmacology*; D017228:Hepatocyte Growth Factor; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D006133:Growth Substances; D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor; D004247:DNA