The large non-collagenous domain (NC-1) of type VII collagen is amino-terminal and chimeric. Homology to cartilage matrix protein, the type III domains of fibronectin and the A domains of von Willebrand factor.
Type VII collagen, the major component of anchoring fibrils, consists of a central collagenous triple-helical segment flanked by non-collagenous domains, NC-1 and NC-2. In this study, we examined the domain organization of human type VII collagen through analysis of deduced amino acid sequences derived from cloned complementary and genomic DNAs, as compared to peptide segments derived from amniotic membrane type VII collagen. The results revealed that the peptide segments derived from the NC-1 domain of type VII collagen could be assigned to the 5' portion of the composite cDNA, indicating that NC-1 resides at the amino terminal end of the molecule. Several sub-domains with homology to adhesive molecules were also identified within NC-1. These protein domains may confer adhesive properties to NC-1, thereby facilitating the binding of type VII collagen to the lamina densa in the cutaneous basement membrane and the anchoring plaques within the dermis.
Christiano
A M
AM
Department of Dermatology, Jefferson Medical College, Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, PA 19107.
Rosenbaum
L M
LM
Chung-Honet
L C
LC
Parente
M G
MG
Woodley
D T
DT
Pan
T C
TC
Zhang
R Z
RZ
Chu
M L
ML
Burgeson
R E
RE
Uitto
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D064236:Cartilage Oligomeric Matrix Protein; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000235:genetics*; D004247:DNA; D016326:Extracellular Matrix Proteins; D005353:Fibronectins / Q000737:chemistry / Q000235:genetics*; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D064235:Matrilin Proteins; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014841:von Willebrand Factor / Q000737:chemistry / Q000235:genetics*
D064236:Cartilage Oligomeric Matrix Protein; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006023:Glycoproteins; D064235:Matrilin Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C577553:TSP5 protein, human; D014841:von Willebrand Factor; D003094:Collagen; D004247:DNA