In vitro recognition of an orf virus early promoter in a vaccinia virus extract.
DNA fragments containing varying lengths of the 5' end of an orf virus early gene (ORF3) and its associated promoter were introduced into sodium deoxycholate-solubilized vaccinia virus extracts capable of initiating transcription in vitro from vaccinia virus early promoters. After separation of the radiolabelled products of the reactions on a 5% polyacrylamide/7 M urea gel, discrete transcripts were detected the sizes of which were consistent with initiation of transcription from the orf virus early promoter. This is the first demonstration in a functional assay of the conservation of early transcriptional promoters between an orthopoxvirus and a parapoxvirus.
Vos
J C
JC
European Molecular Biology Laboratories, Heidelberg, Federal Republic of Germany.
Mercer
A A
AA
Fleming
S B
SB
Robinson
A J
AJ
D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D008969:Molecular Sequence Data; D009923:Orf virus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014616:Vaccinia virus / Q000235:genetics*
D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes