title: [The immunochemical detection of apoprotein dissociation from particles of very low-density lipoproteins in human blood plasma].
abstract: The dissociation of very-low-density lipoprotein (VLDL) apoproteins was studied using immunochemical approaches. The analysis of monospecific antibody binding to apo E, C-II and C-III on VLDL surface showed low apoprotein accessibility for the antibodies while the accessibility of apo C-II and C-III in solution was complete. Lipoprotein preparation dilution resulted in increasing of apo E and C-II accessibility. It was suggested that apoprotein dissociation led to apoprotein cluster dissolving on VLDL surface and higher antigen determinant accessibility. The findings confirmed previous theoretical analysis of apoprotein dissociation.
authors.forename: V V
authors.initials: VV
authors.lastname: Shuvaev
authors.forename: A D
authors.initials: AD
authors.lastname: Dergunov
authors.forename: N V
authors.initials: NV
authors.lastname: Perova
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000302:isolation & purification; D001059:Apoproteins / Q000097:blood* / Q000276:immunology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005454:Fluorescence Polarization; D006801:Humans; D007114:Immunization; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008079:Lipoproteins, VLDL / Q000097:blood* / Q000276:immunology; D011485:Protein Binding; D011817:Rabbits; D012996:Solutions; D013499:Surface Properties
chemical_list: D000906:Antibodies; D001059:Apoproteins; D007140:Immunoglobulin Fab Fragments; D008079:Lipoproteins, VLDL; D012996:Solutions
vernacular_title: Immunokhimicheskoe obnaruzhenie otdeleniia apobelkov ot chastits lipoproteinov ochen' nizkoĭ plotnosti plazmy krovi cheloveka.