Laminin and 67 kD laminin binding protein in mouse B16 melanoma cells and 3T3 fibroblast spheroids.
Multicellular spheroids which promote cell-cell and cell-matrix interactions were prepared in culture with mouse B16 melanoma cells (pigmented or non pigmented) alone or mixed with mouse 3T3 fibroblasts. Their volume and proliferation or necrosis rate were evaluated. As measured by dot blot immunoassay, laminin was mainly produced by fibroblasts rather than by melanoma cells. High levels of laminin B1 chain mRNA were detected only in spheroids composed of 3T3 fibroblasts. The levels of 67 kD laminin binding protein mRNA were high in all cell populations studied here.
Siwek
B L
BL
Department of Histology and Cytology, Faculty of Medicine, University of Liége, Belgium.
Munaut
C
C
Bonjean
K A
KA
Coucke
P H
PH
Noel
A C
AC
De Pauw-Cillet
M C
MC
Foidart
J M
JM
Bassleer
R J
RJ
D016475:3T3 Cells; D000818:Animals; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007700:Kinetics; D007797:Laminin / Q000032:analysis* / Q000235:genetics; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008940:Mitotic Index; D008970:Molecular Weight; D012333:RNA, Messenger / Q000032:analysis; D017474:Receptors, Laminin / Q000032:analysis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D007797:Laminin; D012333:RNA, Messenger; D017474:Receptors, Laminin