A modified method for vascular casting and scanning electron microscopy: its utility in the demonstration of rat pancreatic insulo-acinar and other blood vascular systems.
Monomeric methyl methacrylate resin, supplemented with 1.0% benzoyl peroxide and 1.0% N, N-dimethyaniline, enables good vascular casts useful for scanning electron microscopy when the injected organs or tissues are promptly heated in a microwave processor. The details of this vascular casting and scanning method are described with a clear demonstration of the rat pancreatic blood vascular bed, in particular, its intralobular, extralobular and translobular insulo-acinar portal systems and other vascular routes, including the insulo-venous vessels, which are sinusoidal in nature.
Murakami
T
T
Department of Anatomy, Okayama University School of Medicine, Japan.
Fujita
T
T
Hinenoya
H
H
D000818:Animals; D001808:Blood Vessels / Q000648:ultrastructure*; D008768:Methylmethacrylates; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008872:Microwaves; D010179:Pancreas / Q000098:blood supply* / Q000648:ultrastructure*; D051381:Rats
D008768:Methylmethacrylates