The effects of purified 25-kDa lipase from a clinical isolate of Pseudomonas cepacia in the lungs of rats.
Nanogram quantities of a 25-kDa lipase purified from culture supernatants of Pseudomonas cepacia 90ee, a sputum isolate from a cystic fibrosis (CF) patient, were placed in the lungs of healthy rats. The resulting pathological changes included large amounts of proteinaceous exudate, the accumulation of polymorphonuclear leukocytes and red blood cells, and disorganization of alveolar structure. Pseudomonas cepacia 90ee immobilized in agar beads was also placed in the lungs of rats in a model of chronic infection. This resulted in bronchopneumonia and a milder inflammatory response than that elicited by the purified enzyme.
Lonon
M K
MK
Department of Microbiology, Texas Tech University Health Sciences Center, Lubbock.
Woods
D E
DE
Straus
D C
DC
D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000633:toxicity*; D001996:Bronchopneumonia / Q000139:chemically induced* / Q000473:pathology; D016956:Burkholderia cepacia / Q000201:enzymology*; D005122:Exudates and Transudates; D007249:Inflammation; D008049:Lipase / Q000302:isolation & purification / Q000633:toxicity*; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D011549:Pseudomonas / Q000201:enzymology*; D011650:Pulmonary Alveoli / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D001426:Bacterial Proteins; D008049:Lipase