Evaluation of a microtiter plate fluorescent-antibody assay for rapid detection of human cytomegalovirus infection.
The use of monoclonal antibody (MAb) p63-27, which is reactive with the major immediate-early human cytomegalovirus (HCMV) protein pp72, was explored for the rapid diagnosis of HCMV viruria. The rapid assay detected all but 1 of 19 specimens identified by standard virus isolation methods from 1,676 newborn urine specimens, achieving a sensitivity of 94.5% and a specificity of 100%. The monoclonal antibody recognized 260 randomly obtained clinical isolates of HCMV, indicating the suitability of this reagent for use in screening assays. The sensitivity of the microtiter plate method declined rapidly for specimen from older infants and children with congenital CMV infection and virus-infected children attending a day-care center and was judged to be unacceptable for screening populations in this age group.
Boppana
S B
SB
Department of Pediatrics and Microbiology, University of Alabama, Birmingham 35294-0011.
Smith
R J
RJ
Stagno
S
S
Britt
W J
WJ
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000302:isolation & purification; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique / Q000706:statistics & numerical data; D006801:Humans; D017874:Immediate-Early Proteins; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus