title: Effects of early amino acid administration during total parenteral nutrition on protein metabolism in pre-term infants.
abstract: 1. We investigated the effects of starting amino acid administration on post-natal day 2 on protein turnover and nitrogen balance in appropriate-for-gestational-age, very-low-birth-weight infants. Eighteen infants were divided into two groups. Group A received from day 2 onwards an amino acid solution, whereas group B started on this solution after day 4. Both groups were exclusively parenterally fed, 200 kJ day-1 kg-1 on post-natal days 3 and 4. Group A (birth weight 1.5 +/- 0.3 kg) received 4.6 g of glucose, 1.9 g of fat and 2.3 g of amino acids day-1 kg-1 body weight. Group B (birth weight 1.4 +/- 0.2 kg) received 7.0 g of glucose and 1.9 g of fat day-1 kg-1 body weight. 2. At post-natal day 3, a primed constant infusion of 3 mg of [15N]glycine day-1 kg-1 was given. Protein flux, protein synthesis and protein breakdown were calculated from the 15N enrichment in urinary ammonia. In five out of nine infants in group B no plateau of 15N enrichment in urinary urea could be detected, whereas in group A two out of nine infants did not reach a plateau. For this reason we did not use the end product urea for our calculations. 3. The administration of the amino acids resulted in a higher protein flux (6.9 +/- 1.5 g day-1 kg-1 versus 5.2 +/- 0.9 g day-1 kg-1) and a higher protein synthesis rate (6.0 +/- 1.4 g day-1 kg-1 versus 4.6 +/- 0.8 g day-1 kg-1) in group A.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pediatrics, Erasmus University, Rotterdam, The Netherlands.
authors.forename: R A
authors.initials: RA
authors.lastname: van Lingen
authors.forename: J B
authors.initials: JB
authors.lastname: van Goudoever
authors.forename: I H
authors.initials: IH
authors.lastname: Luijendijk
authors.forename: J L
authors.initials: JL
authors.lastname: Wattimena
authors.forename: P J
authors.initials: PJ
authors.lastname: Sauer
mesh_terms: D000367:Age Factors; D000596:Amino Acids / Q000008:administration & dosage*; D000641:Ammonia / Q000652:urine; D006801:Humans; D007227:Infant Nutritional Physiological Phenomena; D007230:Infant, Low Birth Weight / Q000378:metabolism; D007231:Infant, Newborn; D007234:Infant, Premature / Q000378:metabolism*; D010289:Parenteral Nutrition, Total / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D014508:Urea / Q000652:urine
chemical_list: D000596:Amino Acids; D011506:Proteins; D000641:Ammonia; D014508:Urea