[The isolation of ribosomes from Acholeplasma and the study of their physicochemical characteristics].
Ribosomes are produced from Acholeplasma laidlawii PG-8 and A. laidlawii var. granulum str. 118. It is proved as possible to use the standard method of differential centrifugation for isolation of mollicute ribosomes. The physico-chemical properties of acholesplasma ribosomes are studied. The protein content for A. laidlawii PG-8 amounted to 39.6%, rRNA content--60.4% and for A. laidlawii var. granulum--39.1 and 60.8%, respectively. The RNA: protein ratio equals 1.5:1, the ratio of optic density indicates at 260 and 280 nm--2, that is peculiar to treated preparations of ribosomes. Sedimentation coefficient of acholeplasma ribosomes is 70S. The produced preparations of acholeplasma ribosomes can be used subsequently for creating the system of translation in vitro to study the biosynthesis processes of mollicute protein.
Korobkova
E S
ES
Panchenko
L P
LP
Skripal'
I G
IG
D000128:Acholeplasma laidlawii / Q000737:chemistry / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000032:analysis; D002458:Cell Fractionation; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004926:Escherichia coli / Q000737:chemistry / Q000648:ultrastructure; D012329:RNA, Bacterial / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D012269:Ribosomal Proteins / Q000032:analysis; D012270:Ribosomes / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins
Poluchenie ribosom akholeplazm i issledovanie ikh fiziko-khimicheskikh kharakteristik.