Establishment of a culture system for the study of oligodendroglial development: complementary effects of boiled serum and astrocyte extract.
Mixed glial primary cultures derived from neonatal rat brain were used to isolate the progenitor glial cell with the capacity to differentiate into oligodendroglia or type 2 astrocytes depending on the culture medium. Subcultures composed primarily of this progenitor were utilized, first, to study the regulation of oligodendroglial differentiation by two factors added to chemically defined medium (CDM), i.e. boiled fetal calf serum (FCS) and cellular extract of type 1 astrocytes, and, second, to devise culture conditions that would cause the progenitor cells to differentiate virtually exclusively along oligodendroglial lines (judged by immunologically identified expression of galactocerebroside; GC) and with high specific and total activity of the oligodendroglial enzymes, 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) and glycerol-3-phosphate dehydrogenase (GPDH). Addition of untreated FCS to CDM of the progenitors resulted in 6 days in richly cellular, mixed glial cultures composed of slightly more GFAP-positive astrocytes than oligodendroglia. Addition of boiled FCS to CDM of the progenitors resulted in 6 days in cultures composed almost exclusively (95%) of GC-positive cells and with high specific activity of CNP. This effect of boiled serum, compared to untreated serum, was related to virtual elimination of astrocytes, in the presence of continued differentiation to GC-positive oligodendroglia. Addition of type 1 astrocyte extract as well as boiled FCS to CDM was necessary to generate high specific activity of GPDH. Additionally, the total number of oligodendroglia increased 2-fold when astrocyte extract as well as boiled FCS was added to the CDM.(ABSTRACT TRUNCATED AT 250 WORDS)
Ishii
S
S
Department of Neurology, Harvard Medical School, Boston, MA 02115.
Volpe
J J
JJ
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000737:chemistry*; D015415:Biomarkers; D001790:Blood Physiological Phenomena; D001921:Brain / Q000166:cytology; D002417:Cattle / Q000097:blood / Q000196:embryology; D002457:Cell Extracts / Q000494:pharmacology*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D046508:Culture Techniques / Q000379:methods*; D006358:Hot Temperature; D009419:Nerve Tissue Proteins / Q000032:analysis; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D002457:Cell Extracts; D003470:Culture Media; D009419:Nerve Tissue Proteins