[The effectiveness of lanthanide immunofluorescence and immunoenzyme analyses in differentiating viruses of the tick-borne encephalitis complex].
Comparison of the efficacy of time-resolved fluoroimmunoassay (TR-FIA) and two variants of enzyme immunoassay: conventional (EIA-1) and one using biotin-streptavidin system (EIA-2), in differentiation of viruses of the tick-borne encephalitis complex showed that, their specificity being similar, the TR-FIA method was 2-4 times as sensitive as EIA-2 and 8-32 times as sensitive as EIA-1. When the reactivity of the antigens was assessed by titers, the differentiating capacity was found to be similar and to depend upon the specificity of "sandwich"-forming antibodies and the sensitivity of the immunoassay. When the reactivity of the antigens was assessed by relative reactivity in 4 test systems of different compositions, the differentiating capacity of TR-FIA was higher than that of EIA-1 and EIA-2 which allowed additional differentiation of 2 groups of viruses on the basis of qualitative characteristics of reactivity.
Pomelova
V G
VG
Zavodchikova
E N
EN
Lavrova
N A
NA
Kovalenko
V A
VA
Gaĭdamovich
S Ia
SIa
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000302:isolation & purification; D000956:Antigens, Viral / Q000032:analysis; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D015200:Fluoroimmunoassay / Q000379:methods*; D007124:Immunoenzyme Techniques; D008674:Metals, Rare Earth; D051379:Mice; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D008674:Metals, Rare Earth
Effektivnost' lantanidnogo immunofliuorestsentnogo i immunofermentnogo analizov pri differentsiatsii virusov kompleksa kleshchevogo éntsefalita.