title: Distribution and substrate specificity of intracellular proteolytic processing enzyme(s) for paramyxovirus fusion glycoproteins.
abstract: Intracellular proteolytic processing of fusion glycoprotein precursors (F0) of paramyxoviruses, i.e. a virulent strain of Newcastle disease virus (NDV), parainfluenza virus type 3 (PIV3) and simian virus 5 (SV5), was examined in NALM6 and BSC40 cells and compared with that in LLCMK2 cells to investigate the distribution of the virus-activating protease(s) among the cells and its substrate specificity. BSC40 cells lack a processing endoprotease of the neuropeptide precursor, pro-opiomelanocortin (POMC), which possesses multiple cleavage sites at pairs of basic residues, Lys-Arg and Arg-Arg, a motif similar to that found in the cleavage site of the F0 proteins. In NALM6 cells, only small amounts of the F0 protein of virulent NDV was cleaved whereas those of PIV3 and SV5 were efficiently cleaved. In BSC40 cells the F0 proteins of these three viruses were cleaved normally as well as in LLCMK2 cells. The processing inhibitors monensin, chloroquine and A23187 suppressed the F0 cleavage in the three cell types. These results indicate that both NALM6 and BSC40 cells possess virus-activating proteases similar to that of LLCMK2 cells, but suggest that the enzyme of NALM6 may be slightly different in its substrate specificity from those of BSC40 and LLCMK2. The results also suggest that the virus-activating proteases are different in their distribution and substrate specificity from the processing enzyme of POMC.
authors.affiliation: Department of Bacteriology, Hiroshima University School of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kawahara
authors.forename: X Z
authors.initials: XZ
authors.lastname: Yang
authors.forename: T
authors.initials: T
authors.lastname: Sakaguchi
authors.forename: K
authors.initials: K
authors.lastname: Kiyotani
authors.forename: Y
authors.initials: Y
authors.lastname: Nagai
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism*; D008565:Membrane Proteins; D008985:Monensin / Q000494:pharmacology; D009522:Newcastle disease virus / Q000378:metabolism; D010224:Parainfluenza Virus 3, Human / Q000378:metabolism; D010252:Paramyxoviridae / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012697:Serine Endopeptidases; D013379:Substrate Specificity; D014760:Viral Fusion Proteins / Q000378:metabolism*; D014774:Virulence; D014775:Virus Activation
chemical_list: D008565:Membrane Proteins; D011498:Protein Precursors; D014760:Viral Fusion Proteins; D000001:Calcimycin; D002738:Chloroquine; D008985:Monensin; D010450:Endopeptidases; D012697:Serine Endopeptidases; C026515:type I signal peptidase