title: Modulation of kidney function in conscious Pekin ducks by atrial natriuretic factor.
abstract: The influence of avian atrial natriuretic factor (ANF) on renal function was examined in conscious saltwater-acclimated Pekin ducks undergoing a steady state diuresis maintained by iv infused isotonic avian Krebs-Ringer solution at a rate of 1.0 ml/min. Synthetic chicken ANF (chANF) was applied iv at doses of 10, 50, and 90 ng/min.kg BW for 10 min and caused dose-dependent transient increases in urine flow, osmolal excretion, glomerular filtration rate, effective renal plasma flow, and fractional water clearance at decreased urinary osmolality. Using receptor autoradiography, binding sites for [125I]Bolton-Hunter-labeled chANF [( 125I]BH-chANF) were localized in both the reptilian-type glomeruli and the collecting duct system throughout the duck kidney. A RRA for [125I]BH-chANF, established using an enriched kidney membrane fraction, indicated that unlabeled chANF and human ANF competitively displaced [125I]BH-chANF with comparable potencies. ANF-induced modulation of renal salt and water elimination via glomerular and tubular receptor interactions is consistent with the concept that this hormone has a physiological role in avian volume homeostasis.
authors.affiliation: Max Planck Institute for Physiological and Clinical Research, W. G. Kerckhoff Institute, Bad Nauheim, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schütz
authors.forename: D A
authors.initials: DA
authors.lastname: Gray
authors.forename: R
authors.initials: R
authors.lastname: Gerstberger
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism / Q000494:pharmacology*; D001345:Autoradiography; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004372:Ducks; D005260:Female; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007444:Inulin; D007457:Iodine Radioisotopes; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008297:Male; D009994:Osmolar Concentration; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012079:Renal Circulation / Q000187:drug effects; D013997:Time Factors; D010130:p-Aminohippuric Acid
chemical_list: D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D007444:Inulin; D017461:Receptors, Atrial Natriuretic Factor; D010130:p-Aminohippuric Acid