[The characteristics of lactate oxidation in the tissue mitochondria of rats of different ages].
The parameters of respiration (V3, V4) and phosphorylation (the respiration control, ADP/O) have been studied using lactate as a substrate (obligatory with NAD addition) close by meaning to pyruvate on the liver and heart mitochondrion and homogenates of newborn rats. In 20-days and adult rats the mitochondria and homogenates oxidize the lactate (with NAD) with higher rate V4 but with lower value of respiration control as compared with the newborn animals. Simultaneously, a high activity of mitochondrial NADH-oxidase, oxidizing NADH, formed in the reaction of lactate dehydrogenase not connected with ATP synthesis. The role of mitochondrial NADH-oxidase are discussed as a factor increasing lactate oxidation, removing tissue lactate and activating the age dependent energy metabolism.
Makhmudov
E S
ES
Akhmerov
R N
RN
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D008929:Mitochondria, Heart / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D051381:Rats
D007773:Lactates; D019344:Lactic Acid
Osobennosti okisleniia laktata v mitokhondriiakh tkaneĭ krys raznogo vozrasta.