Reversal of doxorubicin resistance by tetrandrine in Chinese hamster ovary cell line.
Tetrandrine (Tet) 0.5 microgram.ml-1 and 1 microgram.ml-1 potentiated 2.88- and 4.3-fold growth-inhibitory effects of doxorubicin (Dox) in Chinese hamster ovary cell line (CHO), respectively, while Tet 1 microgram.ml-1 and 2.5 micrograms.ml-1 potentiated 7.3- and 8.4-fold in its resistant cell line (CHO/Dox), respectively. The colony-forming efficiencies were reduced in CHO and CHO/Dox when the cells were treated with noncytotoxic doses of Tet 2.5 micrograms.ml-1 and 5 micrograms.ml-1 in combination with different concentration of Dox. Increase in accumulation of Dox in CHO/Dox cells was shown by fluorometry. The result indicated that Tet reversed the resistance to Dox in CHO/Dox cells.
He
Q Y
QY
Department of Biology, Beijing Normal University, China.
Jiang
B
B
Zhang
H Q
HQ
Xue
S B
SB
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D044182:Benzylisoquinolines; D016466:CHO Cells / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D004357:Drug Synergism
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D044182:Benzylisoquinolines; C009438:tetrandrine; D004317:Doxorubicin