R5020 enhances PGE2 stimulated steroidogenesis in cultured rat granulosa cells.
Progesterone biosynthesis and metabolization to 20 alpha-hydroxyprogesterone was stimulated in granulosa cells cultured in the presence of 20 ng/ml of follicle stimulating hormone (FSH) or increasing concentrations of PGE2 (10(-9)-10(-7)M). Concurrent treatment with the synthetic progestin R5020 (10(-6) M) enhanced the FSH or PGE2 stimulated progesterone and 20 alpha-hydroxyprogesterone accumulation in culture media, as well as delta 5-3 beta-hydroxysteroid dehydrogenase activity in granulosa cell homogenates. These findings may represent another example of an autocrine control mechanism in which the steroidogenic product of the granulosa cell exerts an ultra-short loop regulation of its own production.
Fanjul
L F
LF
Departamento de Endocrinología Celular y Molecular, Facultad de Ciencias Médicas y de la Salud, Universidad de Las Palmas de G.C., Spain.
González
J
J
Santana
P
P
Estévez
F
F
Quintana
J
J
Ruiz de Galarreta
C M
CM
D004092:20-alpha-Dihydroprogesterone / Q000096:biosynthesis; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology*; D004357:Drug Synergism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis*; D011397:Promegestone / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013268:Stimulation, Chemical
D004092:20-alpha-Dihydroprogesterone; D011374:Progesterone; D005640:Follicle Stimulating Hormone; D011397:Promegestone; D015096:3-Hydroxysteroid Dehydrogenases; C031954:delta(5)-3 beta-hydroxysteroid dehydrogenase; D015232:Dinoprostone