Enhanced phagocytosis and intracellular killing of Candida albicans by GM-CSF-activated human neutrophils.
Little is known about effector cell modulation regarding phagocytosis and candidacidal activities of human neutrophils in response to granulocyte-macrophage colony stimulating factor (GM-CSF). In neutrophil monolayer assays, pre-treatment of cells with ultrapure GM-CSF at 40 Units ml-1 for a minimum of 1 h showed a significant enhancement of phagocytosis of Candida albicans (phagocytic index of 2.6 compared with 1.6 for unactivated neutrophils) after a 20 min ingestion period (P < 0.001). By counting viable colonies 50% of intracellular yeast cells were killed by non-activated neutrophils following internalization for 2 h. Neutrophils activated for 2 h with 40 Units ml-1 of GM-CSF killed 90% of intracellular blastoconidia (P < 0.02). These results demonstrate that in in vitro assays GM-CSF enhances phagocytosis and intracellular killing of C. albicans.
Richardson
M D
MD
Department of Dermatology, University of Glasgow, UK.
Brownlie
C E
CE
Shankland
G S
GS
D002176:Candida albicans / Q000276:immunology*; D002478:Cells, Cultured; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology
D016178:Granulocyte-Macrophage Colony-Stimulating Factor