title: Increase in spontaneous platelet aggregation in beta-thalassemia/hemoglobin E disease: a consequence of splenectomy.
abstract: Clinical symptoms related with disturbances of the circulatory system are often observed in beta-thalassemia/hemoglobin E (beta-thal/HbE) patients after splenectomy. Pulmonary thrombosis is one of the important contributing factors. However, the pathogenesis of this phenomenon was not known. Previous studies on platelet functions were controversial as platelet-rich plasma (PRP) was employed for all of the studies. By centrifugation, most of the hyperactive platelets were excluded before platelet aggregation tests were performed. Besides, the role of red cells related to platelet aggregation was not investigated. In this study, a platelet function test was designed to avoid these two handicaps of previous work as mentioned, by using whole blood from 15 normal and 40 beta-thal/HbE patients (15 nonsplenectomized and 25 splenectomized) to study spontaneous platelet aggregation. The principle of the test was to evaluate platelet number in whole blood by electronic platelet counter at time 0 (45 minutes after blood collection) and this number was used as 100% of free unaggregated platelets. Then the same specimen of whole blood was incubated at 37 degrees C with continuous stirring by magnetic stirrer in an aggregometer for 8 minutes; at 1 minute intervals free unaggregated platelets were evaluated and calculated as a percentage of the initial control value. The results indicated increased spontaneous platelet aggregation in whole blood of post-splenectomized beta-thal/HbE patients. The residual free platelet number were 24% at 8 minutes after incubation. Effects of red blood cells on spontaneous platelet aggregation were studied by mixing autologous beta-thal/HbE red cells obtained from splenectomized and non-splenectomized patients with platelet rich plasma.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Opartkiattikul
authors.forename: Y
authors.initials: Y
authors.lastname: Funahara
authors.forename: S
authors.initials: S
authors.lastname: Fucharoen
authors.forename: P
authors.initials: P
authors.lastname: Talalak
mesh_terms: D000328:Adult; D000554:Ambulatory Care Facilities; D001791:Blood Platelet Disorders / Q000097:blood* / Q000453:epidemiology / Q000209:etiology; D002498:Centrifugation; D004109:Dilazep / Q000494:pharmacology; D005260:Female; D006446:Hemoglobin E; D006453:Hemoglobinopathies / Q000150:complications / Q000235:genetics / Q000601:surgery*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects; D011183:Postoperative Complications / Q000097:blood* / Q000453:epidemiology / Q000209:etiology; D013156:Splenectomy / Q000009:adverse effects*; D013785:Thailand / Q000453:epidemiology; D013997:Time Factors; D017086:beta-Thalassemia / Q000150:complications / Q000235:genetics / Q000601:surgery*
chemical_list: D006446:Hemoglobin E; D004109:Dilazep