Isolation of a new ligand-carrying casein fragment from bovine mammary gland microsomes.
Whilst looking for components involved in retinol metabolism in secreting mammary gland cells, a 12 kDa protein was isolated. This protein had bound a ligand with characteristics of retinol. N-Terminal sequencing and amino acid analysis showed that this protein is highly homologous with an alpha-s1-casein fragment. No ligand was found for beta-lactoglobulin, previously thought to be involved in retinol metabolism.
Neuteboom
B
B
Centro Studio Alimentazione Animali, CNR, Torino, Italy.
Giuffrida
M G
MG
Conti
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000302:isolation & purification*; D002417:Cattle; D003600:Cytosol / Q000737:chemistry; D005260:Female; D008321:Mammary Glands, Animal / Q000737:chemistry*; D008861:Microsomes / Q000737:chemistry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification*; D012177:Retinol-Binding Proteins / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid
D002364:Caseins; D010446:Peptide Fragments; D012177:Retinol-Binding Proteins