Acidic fibroblast growth factor delays in vitro ischemia-induced intracellular calcium elevation in gerbil hippocampal slices: a sign of neuroprotection.
Using microfluorometry, effects of acidic fibroblast growth factor (aFGF) on the in vitro ischemia-induced intracellular calcium elevation were investigated in gerbil hippocampal slices at 35 degrees C. When slices were superfused with hypoxic and glucose-free medium, the mean latency of the in vitro ischemia-induced calcium elevation was 209 +/- 51 s. The addition of aFGF in medium (25 micrograms/l) delayed the calcium elevation throughout the experiments: the mean latency was 541 +/- 94 s. This retardation in calcium elevation may be indicative of neuroprotective nature of aFGF.
Mitani
A
A
Department of Physiology, Ehime University, School of Medicine, Japan.
Oomura
Y
Y
Yanase
H
H
Kataoka
K
K
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D005849:Gerbillinae; D006624:Hippocampus / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007511:Ischemia; D007700:Kinetics; D008297:Male; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011712:Pyramidal Tracts / Q000187:drug effects / Q000378:metabolism
D016220:Fibroblast Growth Factor 1; D002118:Calcium