[Excitant effect of sodium suberogorgin on isolated rabbit ileum].
Suberogorgin was isolated from Gorgoniae suberogorgia sp from South China Sea. The isolated rabbit ileum contracted in a concentration-dependent manner by suberogorgin-Na (Sub) 2.13 mumol.L-1 or higher. Sub 213 mumol.L-1 had no effect on the relaxation induced by norepinephrine and isoprenaline 10-100 mumol.L-1 on ileum. The stimulation of Sub on ileum was antagonized by atropine 56 mumol.L-1 or indometacin 0.35 mmol.L-1 and was partly blocked by indometacin 0.17 mmol.L-1, but was not affected by diphenhydramine 10 mumol.L-1. On the contracted ileum induced by neostigmine 1.7 mumol.L-1, acetylcholine 0.1 mumol.L-1 increased the tension but Sub 213 mumolL-1 did not. Sub obviously inhibited the acetylcholinesterase (AChE) from mouse brain hemogenates in vitro. Its pI50 (negative logarithm of molar concentration causing 50% inhibition of AChE) was 4.18, while the pI50 of neostigmine was 4.75. The results indicated that the ileum-excitant action of Sub was chiefly related to the inhibition of AChE.
Xu
S B
SB
Department of Biology, Zhongshan University, Guangzhou, China.
Peng
W D
WD
Hu
Y T
YT
Wang
Y F
YF
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects; D009388:Neostigmine / Q000494:pharmacology; D011817:Rabbits; D012717:Sesquiterpenes / Q000494:pharmacology*
D012717:Sesquiterpenes; C049055:suberogorgin; D009388:Neostigmine; D000110:Acetylcholinesterase