Measurement of recombinant interferon levels by high performance immunoaffinity chromatography in body fluids of cancer patients on interferon therapy.
The technique of high performance immunoaffinity chromatography was used to measure the levels of recombinant interferon in chronic lymphocytic leukaemia patients enrolled in a phase II recombinant interferon clinical trial. The technique employed a short high pressure chromatography column packed with minute glass beads which had monoclonal antibody, directed against recombinant alpha interferon, immobilized to their surface. This system was used to measure interferon levels in a variety of different human body fluids. A good correlation was found when interferon levels, detected by chromatographic separation, were compared to levels obtained by a conventional radioimmunoassay.
Phillips
T M
TM
Immunochemistry Laboratory, George Washington University Medical Center, Washington, D.C. 20037.
D000328:Adult; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000276:immunology; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D007370:Interferon Type I / Q000032:analysis* / Q000627:therapeutic use; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D011994:Recombinant Proteins; D012015:Reference Standards; D012463:Saliva / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D019809:Streptavidin
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D007202:Indicators and Reagents; D007370:Interferon Type I; D011994:Recombinant Proteins; D019809:Streptavidin