title: Expression of cloned aromatic L-amino acid decarboxylase in Xenopus laevis oocytes.
abstract: Sense mRNA coding for bovine adrenal medulla aromatic L-amino acid decarboxylase (AADC) was expressed following microinjection into Xenopus laevis oocytes. The expressed enzyme activity was stereoselective for L-5-hydroxytryptophan and L-DOPA and blocked by NSD-1015 an inhibitor of AADC. Heating the expressed enzyme at 55 degrees C resulted in a parallel loss of activity towards both substrates. Our findings are consistent with the prevailing notion that a single enzyme is able to decarboxylate both substrates in vivo.
authors.affiliation: Department of Pharmacology, Ohio State University College of Medicine, Columbus 43210.
authors.forename: K P
authors.initials: KP
authors.lastname: Gudehithlu
authors.forename: A M
authors.initials: AM
authors.lastname: Duchemin
authors.forename: C P
authors.initials: CP
authors.lastname: Silvia
authors.forename: N H
authors.initials: NH
authors.lastname: Neff
authors.forename: M
authors.initials: M
authors.lastname: Hadjiconstantinou
mesh_terms: D000311:Adrenal Glands / Q000201:enzymology; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000096:biosynthesis* / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015723:Gene Library; D007700:Kinetics; D008845:Microinjections; D009865:Oocytes / Q000502:physiology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000008:administration & dosage / Q000378:metabolism*; D014158:Transcription, Genetic; D014982:Xenopus laevis
chemical_list: D012333:RNA, Messenger; D004247:DNA; D001142:Aromatic-L-Amino-Acid Decarboxylases