A novel panel of monoclonal antibodies against beta-galactosidase of Escherichia coli and its versatility for detection of recombinant expression products.
A panel of seven mouse monoclonal antibodies (BG-O1 - BG-07) raised against beta-galactosidase (beta-gal) from E. coli was characterized in respect of their binding to beta-gal and to fusion proteins. The antibodies were beta-gal specific, recognized six different antigenic determinants on beta-gal molecule and some of them inhibited catalytical activity of the enzyme. The antibodies reacted with C-terminal beta-gal fusion proteins in the native state as well as after Western blotting. BG-02 antibody was successfully used for immunofluorescence detection of cells transfected with vectors containing the lacZ gene.
Slavícková
A
A
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Dráber
P
P
Dráberová
E
E
Dráber
P
P
Viklický
V
V
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification*; D014162:Transfection; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase