Identification of an S-antigen-like molecule in human choroid plexus and cerebrospinal fluid.
Sensitisation to retinal S-antigen has been implicated in the pathogenesis of several clinical forms of posterior uveitis. S-antigen-like molecules have recently been demonstrated in the brain and choroid plexus of experimental animals. We used a panel of four monoclonal antibodies (MAbs), MAbF4-C1, MAbC10-C10, MAbA2-G5 and MAbA9-C6, which define specific epitopes in the amino, mid and carboxyl terminal portions of S-antigen in order to identify an S-antigen-like molecule in human choroid plexus and cerebrospinal fluid (CSF). Three MAbs, MAbF4-C1, MAbC10-C10 and MAbA9-C6, localised an S-antigen-like molecule to the cytoplasm of the epithelial cells of the human choroid plexus. Polymerase chain reaction of cDNA from choroid plexus verified the presence of S-antigen homologues in the choroid plexus. The presence of an S-antigen-like molecule in the CSF was demonstrated by western blots in seven CSF samples from patients with a variety of neuropathological disorders. It is proposed that immunological cross-reactivity and biochemical similarity between retinal S-antigen and an S-antigen-like molecule in human choroid plexus and CSF could form a basis for neurological manifestations observed in certain clinical forms of uveitides.
Dua
H S
HS
Retina Service, Wills Eye Hospital, Philadelphia, PA 19107.
Reyes
P F
PF
Barrett
J A
JA
Abrams
M S
MS
Schwarting
R
R
Craft
C M
CM
Donoso
L A
LA
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis* / Q000134:cerebrospinal fluid; D019393:Arrestin; D001324:Autoantigens / Q000032:analysis* / Q000134:cerebrospinal fluid; D015153:Blotting, Western; D002831:Choroid Plexus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000032:analysis* / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010726:Phosphodiesterase Inhibitors / Q000032:analysis / Q000134:cerebrospinal fluid; D016133:Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D000941:Antigens; D019393:Arrestin; D001324:Autoantigens; D005136:Eye Proteins; D010726:Phosphodiesterase Inhibitors