The ELAM-1 ligand sialosyl-Le(X) is present on Langerhans cells isolated from stratified epithelium.
In this study we show the expression of the newly identified carbohydrate ligand, sialosyl-Le(X) on Langerhans cells. The receptor for sialosyl-Le(X) is the endothelial leukocyte adhesion molecule-1 (ELAM-1) present on activated endothelial cells. Using flow cytometry, Langerhans cells were selected due to positivity for an antibody against CD1a and low orthogonal light scatter. The CD1a antigen stained by the OKT6 antibody is considered a maturational marker of Langerhans cells in agreement with the specific labeling of dendritic cells in the epithelium only. Double immunostaining (OKT6/anti-sialosyl-Le(X)) using flow cytometry and immunohistochemistry demonstrated that almost all OKT6-positive cells in normal stratified epithelium expressed sialosyl-Le(X). Conversely, by immunohistochemistry of oral epithelium with acute inflammation, additional dendritic cells negative for OKT6 were found to express sialosyl-Le(X). In addition, sialosyl-Le(X)-positive but not OKT6-positive dendritic cells were found in the submucosa. These findings indicate that the carbohydrate antigen sialosyl-Le(X) is expressed earlier than the CD1a antigen in the maturation of the Langerhans cell lineage. Future studies should aim at investigating the importance of adhesion between sialosyl Le(X) and ELAM-1 in epithelial recruitment of Langerhans cells.
Nylander
K
K
Department of Oral Pathology, University of Umeå, Sweden.
Larsen
J K
JK
Larsen
J
J
Clausen
H
H
Bonding
N
N
Dabelsteen
E
E
D015703:Antigens, CD / Q000378:metabolism; D018949:Antigens, CD1; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002469:Cell Separation; D002610:Cheek; D019040:E-Selectin; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005891:Gingivitis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007801:Langerhans Cells / Q000378:metabolism*; D016256:Lewis X Antigen / Q000378:metabolism*; D008024:Ligands; D009061:Mouth Mucosa / Q000166:cytology / Q000378:metabolism*
D015703:Antigens, CD; D018949:Antigens, CD1; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016256:Lewis X Antigen; D008024:Ligands; C069719:sialosyl dimeric Le(x) antigen