Complementation of a DNA repair deficiency in six human tumor cell lines by chromosome 11.
Human tumor cells, after x-irradiation during the G2 phase of the cell cycle, show an abnormally high frequency of persistent chromatid breaks and gaps resulting from deficient DNA repair. Addition of a single human chromosome 11 from normal fibroblasts by micro-cell fusion to cell lines from six different tumors resulted in efficient repair of the radiation-induced damage to the level in normal cells. For one of the cell lines, addition of the long arm of chromosome 11 was sufficient to restore repair efficiency. In four of the six tumor lines, restoration of efficient DNA repair by chromosome 11 was associated with tumor suppression in nude mice. These results suggest that chromosome 11 carries a DNA repair gene or genes that complement the repair deficiency of tumor cells and that this gene for at least one tumor is localized to the long arm.
Parshad
R
R
Department of Pathology, Howard University College of Medicine, Washington, DC 20059.
Price
F M
FM
Oshimura
M
M
Barrett
J C
JC
Satoh
H
H
Weissman
B E
BE
Stanbridge
E J
EJ
Sanford
K K
KK
D002292:Carcinoma, Renal Cell / Q000235:genetics; D002460:Cell Line; D002880:Chromosomes, Human, Pair 11; D002881:Chromosomes, Human, Pair 12; D004260:DNA Repair / Q000235:genetics*; D004171:Diploidy; D005260:Female; D005816:Genetic Complementation Test; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics; D009369:Neoplasms / Q000235:genetics*; D012208:Rhabdomyosarcoma / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics; D009396:Wilms Tumor / Q000235:genetics