DNA hydrolysis by rare-earth metal ions.
Plasmid DNA and poly(dA) are cleaved by rare-earth(III) ions at pH 7-8 and 50 degrees C. The cleavage has been confirmed by prompt conversion of supercoiled pBR 322 plasmid DNA (Form I) to a relaxed Form II. Furthermore, degradation of poly(dA) to shorter oligonucleotides is clearly evidenced by HPLC. A possible application of the metal ions (and their complexes) to artificial nucleases is indicated.
Matsumoto
Y
Y
Institute of Materials Science, University of Tsukuba, Ibaraki, Japan.
Komiyama
M
M
D002384:Catalysis; D004247:DNA / Q000187:drug effects*; D006868:Hydrolysis; D007477:Ions; D008674:Metals, Rare Earth / Q000737:chemistry / Q000494:pharmacology*; D010957:Plasmids
D007477:Ions; D008674:Metals, Rare Earth; D004247:DNA