[Preliminary study on the role of macrophage in processing and presenting antigens of Schistosoma japonicum].
The horseradish peroxidase labeled affinity purified mice anti-UEA (Urea soluble egg antigen of Schistosoma japonicum) antibody was used in enzyme-linked immunoelectrotransfer blot (EITB) to monitor the changes after UEA being processed by M phi. The immune responsive peptides were detected in the culture supernatant and homogenate of M phi pulsed with UEA in vitro (M phi+). After processing by M phi the high molecular weight UEA was cleaved into low molecular weight peptides, as shown, by the reactive bands. They markedly differed from that native UEA or trypsin-digested UEA. The bands of M phi supernatant and homogenate showed similarity with certain quantitative differences. According to the result described above, we considered: 1. UEA could be processed into smaller pieces by M phi, the style of processing is cleavage. 2. The processed peptides might be released to extracellular environment.
Fang
X H
XH
Department of Parasitology, Sun Yat-sen University of Medical Sciences, Guangzhou.
Rong
G
G
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth / Q000276:immunology*; D015151:Immunoblotting; D008264:Macrophages / Q000276:immunology*; D012549:Schistosoma japonicum / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth