Vasoactive intestinal peptide stimulates immunoglobulin production and growth of human B cells.
The effect of vasoactive intestinal peptide (VIP) on human lymphoblastoid B cell lines and tonsil B cells was studied. VIP increased immunoglobulin production and proliferation by lymphoblastoid B cell line, GM-1056, in a dose-dependent manner. As little as 10(-12) M of VIP was effective, and higher concentrations of VIP induced an approximately five-fold increase in IgA production. Moreover, this enhancement was blocked by VIP antagonist. Similarly, VIP enhanced IgM and IgG production by other lymphoblastoid B cell lines, CBL and IM-9, respectively. In contrast to VIP, another neuropeptide substance P (SP) or somatostatin failed to enhance immunoglobulin production and thymidine uptake. VIP also enhanced IgA production and thymidine uptake by purified tonsil B cells. However, in contrast to B cell lines, VIP failed to enhance IgM and IgG production by tonsil B cells. SP or somatostatin failed to enhance immunoglobulin production or thymidine uptake by tonsil B cells. These results indicate that VIP acts as B cell stimulatory factor and that VIP may also have preferential effect on IgA production on tonsil B cells.
Ishioka
C
C
Department of Paediatrics, Faculty of Medicine, Kyoto University Hospital, Japan.
Yoshida
A
A
Kimata
H
H
Mikawa
H
H
D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002455:Cell Division; D004247:DNA / Q000187:drug effects; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis*; D011964:Receptors, Gastrointestinal Hormone / Q000037:antagonists & inhibitors; D018005:Receptors, Vasoactive Intestinal Peptide; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014660:Vasoactive Intestinal Peptide / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D007070:Immunoglobulin A; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; C048507:vasoactive intestinal peptide, 4-chloro-Phe(6)-Leu(17)-; D014660:Vasoactive Intestinal Peptide; D004247:DNA; D013936:Thymidine