Immunohistochemical demonstration of the Epstein-Barr virus-encoded latent membrane protein in paraffin sections of Hodgkin's disease.
Paraffin sections from 46 cases of Hodgkin's disease were examined for the presence of the Epstein-Barr virus (EBV)-encoded latent membrane protein (LMP) using a sensitive (double layer alkaline phosphatase-anti-alkaline phosphatase) immunohistochemical method. LMP was detected in 22 cases, the majority of positive cases being of nodular sclerosis (12/24), mixed cellularity (6/7), and lymphocyte depletion (3/3) subtypes. Only one of 12 cases of lymphocyte predominant disease was positive. In all cases, reactivity was confined to Hodgkin's and Reed-Sternberg cells. These results provide further evidence for an association between EBV and Hodgkin's disease and indicate that LMP may be readily detected in archival material.
Murray
P G
PG
Department of Histopathology, Good Hope General Hospital, Sutton Coldfield, West Midlands, U.K.
Young
L S
LS
Rowe
M
M
Crocker
J
J
D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000382:microbiology; D008565:Membrane Proteins / Q000032:analysis*; D016612:Paraffin Embedding; D014764:Viral Proteins / Q000032:analysis*
D008565:Membrane Proteins; D014764:Viral Proteins