title: Human decidua is a target tissue for bradykinin and kallikrein: phosphoinositide hydrolysis accompanies arachidonic acid release in uterine decidua cells in vitro.
abstract: The endocrine and intracellular mechanisms regulating prostaglandin precursor release in the uterine decidua during labor are unknown. This in vitro study investigates a potential role for a kallikrein-kinin system in the activation of phospholipid hydrolysis and arachidonic acid release in human decidua cells. Primary cultures of human decidua cells were prelabeled with [3H]inositol or [14C]arachidonic acid to monitor phosphoinositide hydrolysis and prostaglandin precursor release, respectively. Bradykinin (100 nmol/L) stimulated a rapid release of arachidonic acid (within 2 min) associated with an increase in inositol trisphosphate which was detectable after 20 s. Protein kinase C activation by phorbol ester enhanced arachidonic acid release in response to both bradykinin and the Ca++ ionophore A23187 but inhibited bradykinin-stimulated phosphoinositide hydrolysis. Epidermal growth factor also enhanced arachidonate release in response to both bradykinin and A23187. Kallikrein stimulated both phosphoinositide hydrolysis and arachidonic acid release in decidua cells. Kallikrein action was inhibited by the kallikrein protease inhibitor aprotinin and D-Arg[Hyp3Thi5,8,D-Phe7] bradykinin, a B2 receptor antagonist. Bradykinin also stimulated prostaglandin F2 alpha production in both primary decidua cell cultures and fibroblasts in the presence of interleukin-1 beta. These findings are consistent with a mediatory role for bradykinin in the action of kallikrein on decidua cells and suggest that inositol phospholipid hydrolysis is instrumental for arachidonic acid release in response to bradykinin in these cells. This study supports a novel role for a kallikrein-kinin system in the human uterine decidua.
authors.affiliation: Unit of Metabolic Medicine, St. Mary's Hospital Medical School, University of London, England.
authors.forename: M P
authors.initials: MP
authors.lastname: Schrey
authors.forename: J R
authors.initials: JR
authors.lastname: Holt
authors.forename: P A
authors.initials: PA
authors.lastname: Cornford
authors.forename: H
authors.initials: H
authors.lastname: Monaghan
authors.forename: F
authors.initials: F
authors.lastname: al-Ubaidi
mesh_terms: D001095:Arachidonic Acids / Q000378:metabolism*; D001920:Bradykinin / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D003656:Decidua / Q000378:metabolism*; D015237:Dinoprost / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007375:Interleukin-1 / Q000494:pharmacology; D007610:Kallikreins / Q000494:pharmacology*; D007700:Kinetics; D010716:Phosphatidylinositols / Q000378:metabolism*; D011247:Pregnancy; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
chemical_list: D001095:Arachidonic Acids; D007375:Interleukin-1; D010716:Phosphatidylinositols; D000001:Calcimycin; D015237:Dinoprost; D007610:Kallikreins; D013755:Tetradecanoylphorbol Acetate; D001920:Bradykinin