High-performance liquid chromatographic determination of fatty acid binding proteins in rat liver with fluorescence detection.
A highly sensitive, simple and reproducible method for the quantitative determination of fatty acid binding protein in rat liver by post-column high-performance liquid chromatography with fluorescence detection is presented. Fatty acid binding protein in rat liver cytosols is separated by gel-permeation column chromatography, which is followed by fluorescence detection. The detection makes use of the fluorescence enhancement observed when a fluorescent fatty acid probe, dansylundecanoic acid, binds to fatty acid binding protein. The method is rapid, simple to perform and highly sensitive. The method was applied to the determination of fatty acid binding protein in liver from control and hypolipidaemic drug treated rats.
Yamaguchi
M
M
Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
Wada
K
K
Ishida
J
J
Nakamura
M
M
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005260:Female; D008099:Liver / Q000737:chemistry*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence
D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins