[The synthesis of collagenous and noncollagenous proteins by cells from the periodontium].
Synthesis of collagenous and noncollagenous proteins by cells originating from the alveolar bone (AB-cells) and from the periodontal ligament (PDL-cells) of 8 minipigs was analyzed in primary cell cultures. Incorporation of 3H-proline into proteins recovered from cell extracts showed, that 13.7% of protein synthesized by AB-cells and 8.0% of protein synthesized by PDL-cells were collagens. In both cell lines relative amounts of collagens synthesized decreased significantly on subculturing (AB-cells: 9.3%, PDL-cells: 6.0%). Preincubation of 2nd subculture AB-cells with beta-glycerophosphate had no significant effect on collagen synthesis. Considering the fact that relative amounts of collagen recovered from cell extracts represent only 10-20% of collagen recovered from whole culture (i.e. cells and culture medium), we suggest that collagen synthesis is an important component of in vitro-protein synthesis of cells originating from the alveolar bone and the periodontal ligament.
Lang
H
H
Poliklinik für Zahnerhaltung und Parodontologie, Zentrum für Zahn-, Mund- und Kieferheilkunde, Universität Bonn.
Schüler
N
N
Zimmermann
A
A
Bodo
M
M
Brinckmann
J
J
Mertens
T
T
Müller
P K
PK
Nolden
R
R
D000539:Alveolar Process / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured / Q000737:chemistry / Q000378:metabolism; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis*; D010513:Periodontal Ligament / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D010519:Periodontium / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D013552:Swine; D013556:Swine, Miniature
D011506:Proteins; D003094:Collagen
Synthese kollagener und nicht-kollagener Proteine durch Zellen aus dem Parodont.