Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe.
A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures. DNA fragments digested with restriction endonuclease BamHI were separated, transferred and immobilized on filter membranes. A Digoxigenin-labeled probe derived from EHV 1 was used for hybridization. This probe hybridized specifically to sequences of the inverted terminal repeat region which in case of Rac H include a deletion of 0.8 kb. By comparing the different migration patterns after blot hybridization it could be shown that in 65 isolates from cases of abortion the live vaccine strain Rac H was not involved.
Meyer
H
H
Federal Armed Forces Medical Academy, Institute of Microbiology, Munich, Germany.
Hübert
P
P
Schwend
C
C
Eichhorn
W
W
D000034:Abortion, Veterinary / Q000382:microbiology; D000818:Animals; D002460:Cell Line; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D004861:Herpesvirus 1, Equid / Q000235:genetics / Q000302:isolation & purification*; D006734:Horse Diseases / Q000382:microbiology; D006736:Horses; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014765:Viral Vaccines / Q000302:isolation & purification*
D015342:DNA Probes; D004279:DNA, Viral; D014765:Viral Vaccines