Calibration of quantitative histochemical methods: estimation of glycogen content of muscle fibers using the PAS reaction.
A fairly simple method for calibrating microdensitometric histochemical assays is described. The method is based on paired biochemical and histochemical assays on single freeze-dried skeletal muscle fibers which differ widely in their properties. As an example, the method is applied to investigate the validity of the periodic acid-Schiff (PAS) reaction for the microdensitometric estimation of glycogen content. Some problems that may interfere with the calibration are discussed.
van der Laarse
W J
WJ
Laboratory for Physiology, Free University, Amsterdam, The Netherlands.
van Noort
P
P
Diegenbach
P C
PC
D000818:Animals; D003720:Densitometry; D005260:Female; D005612:Freeze Drying; D006003:Glycogen / Q000032:analysis*; D009132:Muscles / Q000033:anatomy & histology / Q000737:chemistry*; D010503:Periodic Acid-Schiff Reaction; D014982:Xenopus laevis
D006003:Glycogen