Identification of a membrane-associated 1-0-alkyl-2-arachidonoyl-sn-glycero-3-phosphocholine hydrolyzing phospholipase A2 in guinea pig 1 epidermis. Implications in the cutaneous biosynthesis of platelet-activating factor.
A membrane-associated 1-0-alkyl-2-arachidonoyl-GPC hydrolyzing phospholipase A2 was identified in guinea pig epidermis. It is regio-specific (associated with the particulate microsomal fraction) and specific for the hydrolysis of 1-0-alkyl-2-arachidonoyl-sn-glycero-3-phosphocholine. It is sensitive to low calcium concentrations suggesting that it may be activated by increasing intracellular calcium. Since ether-linked phospholipids are known to exist in the epidermis, further understanding of the properties of this 1-0-alkyl-arachidonoyl-hydrolyzing PLA2 may allow us to control the generation of 1-0-alkyl-2-lyso-sn-glycero-3-phosphocholine, a key substrate for the generation of the platelet-activating factor in the tissue.
Wong
B
B
Department of Dermatology, School of Medicine, University of California, Davis 95616.
Tang
W
W
Ziboh
V A
VA
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004817:Epidermis / Q000201:enzymology*; D006168:Guinea Pigs; D006868:Hydrolysis; D007425:Intracellular Membranes / Q000201:enzymology; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000201:enzymology; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010742:Phospholipid Ethers / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000096:biosynthesis*
D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; C043051:1-O-hexadecyl-2-arachidonyl-sn-glycero-3-phosphocholine; D010741:Phospholipases A; D054467:Phospholipases A2