title: Specific incorporation of kinetin into eukaryotic and prokaryotic transfer ribonucleic acid molecules.
abstract: We show that kinetin, a non-natural product with strong cytokinin activity, is incorporated into prokaryotic and eukaryotic tRNAs in the exchange reaction catalysed by a putative tRNA-kinetin transglycosylase. We also show that kinetin is specifically incorporated into E. coli tRNA(Tyr) and most probably at position 37. To our knowledge, this is the first report of a nucleic acid base exchange reaction occurring at this position.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan.
authors.forename: J
authors.initials: J
authors.lastname: Barciszewski
authors.forename: D
authors.initials: D
authors.lastname: Otzen
authors.forename: S I
authors.initials: SI
authors.lastname: Rattan
authors.forename: B F
authors.initials: BF
authors.lastname: Clark
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D002384:Catalysis; D004926:Escherichia coli / Q000737:chemistry; D007701:Kinetin; D012329:RNA, Bacterial / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism*; D012365:RNA, Transfer, Tyr / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry
chemical_list: D012329:RNA, Bacterial; D012331:RNA, Fungal; D012365:RNA, Transfer, Tyr; D000225:Adenine; D007701:Kinetin