title: Expression of an active cathepsin B-like protein Sm31 from Schistosoma mansoni in insect cells.
abstract: Recombinant Schistosoma mansoni cathepsin B was produced in insect cells via the baculovirus expression vector system as a 37.5 kDa precursor molecule and a 31 kDa mature enzyme. Extracts prepared from cells infected with the recombinant virus were able to cleave a synthetic dipeptide substrate specific for cathepsin B. Proteolytic activity was inhibited by trans-epoxysuccinyl-L-leucyl-amido (4-guanidino) butane (E64) but not by phenylmethylsulphonyl-fluoride (PMSF), pepstatin and 1,10-phenanthroline. Specific inhibition by diazomethylketone derivatives, which bind covalently to the active centre of cysteinyl proteinases was also demonstrated.
authors.affiliation: Institute of Parasitology, University of Zürich, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Götz
authors.forename: R
authors.initials: R
authors.lastname: Felleisen
authors.forename: E
authors.initials: E
authors.lastname: Shaw
authors.forename: M Q
authors.initials: MQ
authors.lastname: Klinkert
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002401:Cathepsin B / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D005822:Genetic Vectors; D009036:Moths / Q000166:cytology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012550:Schistosoma mansoni / Q000201:enzymology* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D002401:Cathepsin B