Production, characterization, and applications of a murine monoclonal antibody to dog erythrocyte antigen 1.1.
A murine IgM monoclonal antibody, which recognizes dog erythrocyte antigen (DEA) 1.1, has been produced. The antibody correctly identified canine RBC possessing DEA 1.1 in a panel of RBC typed by an independent laboratory. Reactivity of the monoclonal antibody was compared with canine anti-DEA 1.1 antiserum with 163 RBC samples from 145 dogs. Results of agglutination tests with the 2 reagents were in agreement for all samples. A card agglutination test that uses the monoclonal antibody with blood is described. A monoclonal antibody-based test should facilitate blood typing for DEA 1.1 in clinical practice.
Andrews
G A
GA
Department of Veterinary Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506-5605.
Chavey
P S
PS
Smith
J E
JE
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001788:Blood Grouping and Crossmatching / Q000662:veterinary*; D004285:Dogs / Q000097:blood*; D004912:Erythrocytes / Q000276:immunology*; D006825:Hybridomas; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D007519:Isoantigens / Q000097:blood / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D007519:Isoantigens