title: Studies with biotinylated RBC: (1) use of flow cytometry to determine posttransfusion survival and (2) isolation using streptavidin conjugated magnetic beads.
abstract: Methods are reported for the quantitation and isolation of biotinylated red blood cells (B- RBC). The first method is for determination of posttransfusional survival of rabbit RBC by flow cytometry. The survival of B-RBC was measured using both fresh and paraformaldehyde-fixed cells with similar results. The posttransfusion survival of rabbit RBC measured in this way was normal. There was no indication of increased cell destruction due to antibodies directed against B-RBC and no evidence for loss of biotin from circulating cells. The second methodology is for the isolation of B-RBC from blood with streptavidin-coated magnetic beads. At least eighty percent of positive cells were recovered with very few false positives. Both methods may be helpful in the study of resealed erythrocytes.
authors.affiliation: Istituto di Fisiologia Veterinaria e Biochimica, Milan, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Russo
authors.forename: R
authors.initials: R
authors.lastname: Barker-Gear
authors.forename: R
authors.initials: R
authors.lastname: Gates
authors.forename: R
authors.initials: R
authors.lastname: Franco
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D001710:Biotin; D001803:Blood Transfusion; D002469:Cell Separation / Q000379:methods*; D004905:Erythrocyte Aging / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D008280:Magnetics; D008863:Microspheres; D011817:Rabbits; D019809:Streptavidin; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D001710:Biotin; D019809:Streptavidin