title: [The effect of cholesterol on membrane potentials, proliferation and migration of cultivated endothelial cells].
abstract: The influence of cholesterol added as cholesterol containing liposomes [sphingomyelin/cholesterol (1: 1; mol/mol)] or as cholesterol suspensions on cultured bovine aortic endothelial cells was investigated. 10(-6) mol/l cholesterol enhanced membrane potential, 10(-5) mol/l caused depolarisation. The proliferation of the cells was dependent on the concentration influenced in opposite directions too. The proliferation was stimulated by 10(-6) mol/l cholesterol and inhibited by 10(-5) mol/l. The migratory rate of the cells was increased by 10(-6) mol/l and 10(-5) mol/l cholesterol. Our results suggest that exogenous cholesterol is integrated in membranes of the endothelial cells and causes in this way changes of membrane potential, proliferation rate and migratory activity.
authors.affiliation: Fachrichtung Pharmazie, Ernst-Moritz-Arndt-Universität Greifswald.
authors.forename: S
authors.initials: S
authors.lastname: Mende
authors.forename: I
authors.initials: I
authors.lastname: Windeck
authors.forename: K H
authors.initials: KH
authors.lastname: Lichtnow
authors.forename: E
authors.initials: E
authors.lastname: Teuscher
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002784:Cholesterol / Q000494:pharmacology*; D004727:Endothelium / Q000166:cytology; D008081:Liposomes; D008564:Membrane Potentials / Q000187:drug effects; D013109:Sphingomyelins / Q000494:pharmacology
chemical_list: D008081:Liposomes; D013109:Sphingomyelins; D002784:Cholesterol
vernacular_title: Zum Einfluss von Cholesterol auf Membranpotential, Teilung und Migration von kultivierten Endothelzellen.