Interaction of antisense DNA with nucleic acids/proteins.
In order to study interaction of various types of labeled antisense DNAs were prepared. Fluorescein and 2,2,6,6-tetramethypiperidine-N-oxyl were the label molecules, which were introduced to 5'-end of oligonucleotides and their analogs. Interactions of labeled antisense DNAs with nucleic acids or proteins such as HSA, HIG and TF, were studied by UV, fluorescence depolarization spectroscopy, and ESR spectroscopy. Hybrid formation of antisense DNAs with oligonucleotides in solution could be monitored by the increase in fluorescence anisotropy (r) and by intensity change in ESR spectra. When phosphorothioate type antisense molecules anchoring fluorescein (F-OPT) were mixed with proteins, r drastically increased, whereas ODN slightly increased. These results suggest that OPTs have much more affinity for proteins than ODNs.
Murakami
A
A
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Nagahara
S
S
Uematsu
H
H
Otoi
K
K
Haruna
T
T
Ide
H
H
Makino
K
K
D001798:Blood Proteins / Q000737:chemistry*; D003497:Cyclic N-Oxides / Q000737:chemistry; D016373:DNA, Antisense / Q000737:chemistry*; D019793:Fluorescein; D005452:Fluoresceins / Q000737:chemistry; D005454:Fluorescence Polarization; D006801:Humans; D009696:Nucleic Acids / Q000737:chemistry*; D013057:Spectrum Analysis; D013113:Spin Labels; D013873:Thionucleotides / Q000737:chemistry
D001798:Blood Proteins; D003497:Cyclic N-Oxides; D016373:DNA, Antisense; D005452:Fluoresceins; D009696:Nucleic Acids; D013113:Spin Labels; D013873:Thionucleotides; D019793:Fluorescein; C037109:2,2,6,6-tetramethylpiperidine-N-oxide-4-amino-4-carboxylic acid