Thermodynamics of interaction of a fluorescent DNA oligomer with the anti-tumour drug netropsin.
Fluorescence spectroscopy was used to study the interaction between the minor-groove-binding drug netropsin and the self-complementary oligonucleotide d(CTGAnPTTCAG)2 containing the fluorescent base analogue 2-aminopurine (nP). The binding of netropsin to this oligonucleotide causes strong quenching of the 2-aminopurine fluorescence, observed by steady-state as well as time-resolved spectroscopy. From fluorescence titrations, binding isotherms were recorded and evaluated. The parameters showed one netropsin binding site/oligonucleotide duplex and an association constant of about 10(5) M-1 at 25 degrees C, 3-4 orders of magnitude weaker than for an exclusive adenine/thymine host sequence. From the temperature dependence of the association constant the thermodynamic parameters were obtained as delta G = -29 kJ/mol, delta H = -12 kJ/mol and delta S = +55 J.mol-1.K-1 at 25 degrees C. These parameters resemble those of the interaction of poly[(dG-dC).(dG-dC)] with netropsin, indicating a mainly entropy-driven reaction. The amino group of 2-aminopurine, like that of guanine, resides in the minor groove of DNA. Therefore the relatively weak binding of netropsin to d(CTGAnPTTCAG)2 is probably related to partial blockage of the tight fit of netropsin into the preferred minor groove of an exclusive adenine/thymine host sequence.
Patel
N
N
Department of Medical Biochemistry and Biophysics, University of Umeå, Sweden.
Berglund
H
H
Nilsson
L
L
Rigler
R
R
McLaughlin
L W
LW
Gräslund
A
A
D015075:2-Aminopurine; D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D008969:Molecular Sequence Data; D009429:Netropsin / Q000378:metabolism*; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; D013816:Thermodynamics
D005456:Fluorescent Dyes; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; D015075:2-Aminopurine; D009429:Netropsin; D004247:DNA