title: Cell-type-specific responsiveness to cAMP in cell differentiation of Dictyostelium discoideum.
abstract: In Dictyostelium discoideum, both prespore and prestalk differentiation require extracellular cAMP. We investigated the difference in inducibility of the two cell types by cAMP. Previous studies indicate that cAMP added in the early stage of development inhibits prespore differentiation, and this was confirmed using three species of prespore specific mRNAs. By contrast, early treatment with cAMP did not inhibit, but induced the expression of prestalk-specific mRNA. These results indicate that differentiation pathways of the two cell types have different processes in the early stage of development.
authors.affiliation: Department of Botany, Faculty of Science, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology*; D004023:Dictyostelium / Q000166:cytology* / Q000235:genetics; D012331:RNA, Fungal / Q000096:biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D013172:Spores, Fungal / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger; D000242:Cyclic AMP