[Gene amplification with PCR and sequence specific HLA oligonucleotide typing].
Genetic polymorphism in the HLA class II region has been identified by the analysis of the polymerase chain reaction (PCR) products using sequence-specific oligonucleotide (SSO). The PCR-SSO method permits precise and direct analysis of allelic variations with as little as 1 microgram of genomic DNA. The standardized, uniform hybridization and critical wash protocol of the Eurotransplant typing kit enables HLA typing independent of gene expression and quality of lymphocytes. One of the advantages of this technique is that the definition of splits is much better than typing by serology.
Holzberger
G
G
Institut für Immunhämatologie und BSD Hessen, BRD.
Frank
R
R
Boehm
B O
BO
Seidl
S
S
D001483:Base Sequence / Q000235:genetics; D016026:Bone Marrow Transplantation; D005784:Gene Amplification; D005802:Genes, MHC Class II / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006650:Histocompatibility Testing / Q000295:instrumentation*; D006801:Humans; D016030:Kidney Transplantation; D009841:Oligonucleotides / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000295:instrumentation*
D005819:Genetic Markers; D009841:Oligonucleotides
Gen-Amplifikation mit der PCR und Sequenzspezifische HLA-Oligonukleotidtypisierung (SSO).