title: Evolutionarily conserved promoter motifs and enhancer organization in the mouse gene encoding the IIB myosin heavy chain isoform expressed in adult fast skeletal muscle.
abstract: We have isolated the mouse gene for the MHC isoform expressed in adult type IIB (fast-contracting, glycolytic) skeletal muscle fibers, and determined the DNA sequence of the promoter region. This sequence represents the first example of a promoter for a gene encoding an adult-specific isoform of a mammalian skeletal MHC. The proximal 200 bp of the promoter contains several sequence motifs which are identical or very similar to homologous motifs found in the promoters of a family of chicken skeletal MHC genes. Of these, two novel AT-rich sequences may be important for regulation of the promoter. A second feature of the mouse IIB MHC promoter sequence concerns a number of sequence motifs located at ca. -1,000 bp which are organized in a similar fashion in the IIB MHC promoter and a homologous region of promoter of the mouse muscle creatine kinase gene.
authors.affiliation: Département de Biologie moléculaire, Institut Pasteur, Paris.
authors.forename: S
authors.initials: S
authors.lastname: Takeda
authors.forename: D L
authors.initials: DL
authors.lastname: North
authors.forename: M M
authors.initials: MM
authors.lastname: Lakich
authors.forename: S D
authors.initials: SD
authors.lastname: Russell
authors.forename: L S
authors.initials: LS
authors.lastname: Kahng
authors.forename: R G
authors.initials: RG
authors.lastname: Whalen
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003402:Creatine Kinase / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005815:Genetic Code; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D009218:Myosins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003402:Creatine Kinase; D009218:Myosins