title: Rotavirus detection by dot blot hybridization assay using a non-radioactive synthetic oligodeoxynucleotide probe.
abstract: A synthetic oligodeoxynucleotide of 40 nucleotides corresponding to nucleotides 33-72 of the gene coding for the viral protein VP7 of rotavirus, was used as a nucleic acid probe to develop a non-radioactive hybridization method for rotavirus detection. The probe was labelled at the 3' end with biotin-7-dATP. The sensitivity and specificity of the dot blot hybridization assay for rotavirus detection was evaluated with 303 stool specimens. The results indicate that the hybridization assay has a higher sensitivity than both PAGE and EIA. Among the rotavirus strains tested 37 different electropherotypes were found. The results suggest that rotavirus diagnosis by dot hybridization using a non-radioactive probe may become routine laboratory procedure because it is simple, highly specific and very sensitive.
authors.affiliation: Unidad de Virología INTA, Universidad de Chile, Santiago.
authors.forename: J
authors.initials: J
authors.lastname: Fernández
authors.forename: A
authors.initials: A
authors.lastname: Sandino
authors.forename: A
authors.initials: A
authors.lastname: Yudelevich
authors.forename: L F
authors.initials: LF
authors.lastname: Avendaño
authors.forename: A
authors.initials: A
authors.lastname: Venegas
authors.forename: V
authors.initials: V
authors.lastname: Hinrichsen
authors.forename: E
authors.initials: E
authors.lastname: Spencer
mesh_terms: D001483:Base Sequence; D003967:Diarrhea / Q000175:diagnosis; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D011237:Predictive Value of Tests; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis* / Q000737:chemistry; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis*
chemical_list: D015345:Oligonucleotide Probes; D012330:RNA, Double-Stranded; D012367:RNA, Viral