title: [An electrophoretic analysis of human glycoprotein hormones and their subunits].
abstract: Stability of heterodimers of human glycoprotein hormones with gonadotropic and thyrotropic activities in sodium dodecylsulfate (SDS) under non-reducing conditions at low temperature permits to resolve the native molecules of these hormones in SDS-PAG and to distinguish from their dissociated subunits by electrophoretical mobility. The analysis of dimers and alpha-, beta-subunits in one polyacrylamide gel allows to detect certain human glycoprotein hormones and to study some of their physico-chemical properties. Using two polyclonal antisera against human LH and FSH by the Western blot immunoassay it was shown that heterodimers as well as alpha and beta subunits after SDS-PAGE retain antigenic activity of native hormones. The method gave possibility to characterize the specificity of the given sera to different glycoprotein hormones.
authors.forename: M S
authors.initials: MS
authors.lastname: Govorun
authors.forename: T A
authors.initials: TA
authors.lastname: Osipova
authors.forename: S N
authors.initials: SN
authors.lastname: Khil'ko
authors.forename: A V
authors.initials: AV
authors.lastname: Martynov
authors.forename: A A
authors.initials: AA
authors.lastname: Bulatov
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D015292:Glycoprotein Hormones, alpha Subunit / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D015151:Immunoblotting; D007986:Luteinizing Hormone / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D011817:Rabbits
chemical_list: D015292:Glycoprotein Hormones, alpha Subunit; D007106:Immune Sera; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
vernacular_title: Elektroforeticheskiĭ analiz glikoproteinovykh gormonov cheloveka i ikh sub''edinits.