title: High-risk human papillomavirus types in cytologically normal cervical scrapes from Kenya.
abstract: Seventy-seven women with normal cervical cytology on routine visit to a family planning clinic in Nairobi, Kenya, were analysed for genital human papillomavirus (HPV) types by polymerase chain reaction (PCR). We applied a general primer pair (GP60/GP124) recognising sequences conserved among HPV types 6, 11, 16, 18, 31 and 33. Of the 77 specimens tested 15 (19.5%) proved to be positive for genital HPV. Amplification products were examined for the presence of high-risk HPV types by Slot-blot hybridization. Out of the 15 PCR-positive samples, 4 were positive for HPV 16.3 for HPV 18, while 1 contained both HPV 16 and 33. HPV DNA prevalence in this group of women from a "high-risk" area is similar to that in "low-risk" Swedish women but much lower than in cervical cancer samples from the same region.
authors.affiliation: Institute of Microbiology, University Medical School of Debrecen, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Czeglédy
authors.forename: K O
authors.initials: KO
authors.lastname: Rogo
authors.forename: M
authors.initials: M
authors.lastname: Evander
authors.forename: G
authors.initials: G
authors.lastname: Wadell
mesh_terms: D000328:Adult; D001483:Base Sequence; D002584:Cervix Uteri / Q000382:microbiology*; D015344:DNA Probes, HPV; D004277:DNA, Single-Stranded; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007630:Kenya; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012306:Risk; D014626:Vaginal Smears
chemical_list: D015344:DNA Probes, HPV; D004277:DNA, Single-Stranded