Chasing the elusive animal model of late-phase bronchoconstriction: studies in dogs, guinea pigs and rats.
Antigen inhalation in sensitized dogs, guinea pigs and rats resulted in a marked, late-phase, eosinophil-rich, influx of inflammatory cells into the bronchial lumen. Attempts to demonstrate an associated late-phase bronchoconstriction were disappointing. We were unable to demonstrate a late-phase bronchoconstriction in either rats or dogs, even when dogs were pretreated with metyrapone to reduce blood cortisol levels. In ovalbumin-sensitized guinea pigs, challenged with low doses of ovalbumin, we observed an immediate bronchoconstriction, a late-phase bronchopulmonary eosinophilia but no late-phase bronchoconstriction. However, inhalation of very high doses of antigen in mepyramine-treated sensitized guinea pigs did induce a moderate late-phase bronchoconstriction.
Richards
I M
IM
Upjohn Company, Upjohn Laboratories, Kalamazoo, MI 49007.
Griffin
R L
RL
Shields
S K
SK
Reid
M S
MS
Fidler
S F
SF
D000818:Animals; D001200:Ascaris / Q000276:immunology; D001980:Bronchi / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000503:physiopathology*; D004195:Disease Models, Animal; D004285:Dogs; D006168:Guinea Pigs; D008797:Metyrapone / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology; D010047:Ovalbumin / Q000276:immunology; D011657:Pulmonary Eosinophilia / Q000503:physiopathology; D011738:Pyrilamine / Q000494:pharmacology; D051381:Rats; D012129:Respiratory Function Tests
D010047:Ovalbumin; D011738:Pyrilamine; D008797:Metyrapone