title: The phylogenetic relationship and complete nucleotide sequence of human papillomavirus type 35.
abstract: The 7851-bp nucleotide sequence of human papillomavirus (HPV) type 35 was determined. HPV 35 is associated with high-grade cervical intraepithelial neoplasia and invasive carcinomas. From the HPV 35 sequence, open reading frames encoding putative proteins E6, E7, E1, E2, E4, E5, L2, and L1, common to other mucosal HPV types, were identified. Structural and control elements present in the long control region (LCR) conserved among other mucosal HPV types were also present in HPV 35. Analysis of the LCR revealed an additional 20-bp sequence element present in all HPV types associated with malignant proliferation. To further classify HPV 35 with regard to oncogenic potential, phylogenetic analysis of the E6 and E7 proteins from the anogenital HPV types 6, 11, 16, 18, 31, 33, 35, 39, 43, 44, 45, and 51 was performed. This analysis indicated three distinct HPV subgroups; those associated with benign lesions and two branches of those HPV types more often associated with malignant proliferation. HPV 35 is most closely related to HPV types 31 and 16.
authors.affiliation: Syngene Inc., San Diego, California 92121.
authors.forename: J E
authors.initials: JE
authors.lastname: Marich
authors.forename: A V
authors.initials: AV
authors.lastname: Pontsler
authors.forename: S M
authors.initials: SM
authors.lastname: Rice
authors.forename: K A
authors.initials: KA
authors.lastname: McGraw
authors.forename: T W
authors.initials: TW
authors.lastname: Dubensky
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D014764:Viral Proteins; C059731:oncogene protein E7, Human papillomavirus type 16