[The first cholera case diagnosed early in the clinical laboratory by DNA probe method].
An alkaline-phosphatase-labelled synthetic oligonucleotide probe was applied to detect the structural gene of cholera enterotoxin (ctx). This DNA probe has a complementary base sequence to 30 base of the CT-A subunit. This method was, for the first time, applied to diagnosis of a diarrheal patient. The probe detected ctx rapidly and simply as compared with reversed passive latex agglutination (RPLA) and Beads-ELISA. The cfu minimal dose for detection with the probe was about 10(6-7)/ml. This method can be easily performed in any clinical laboratory because the procedure is safe, simple and rapid (it can be completed within about 3 hours).
Miyagi
K
K
Quarantine Station, Osaka Airport.
Omura
K
K
Noda
K
K
Yoshida
A
A
Mori
H
H
Suzuki
N
N
Takai
S
S
Matsumoto
Y
Y
Hayashi
K
K
Yoh
M
M
D000328:Adult; D002771:Cholera / Q000175:diagnosis* / Q000453:epidemiology; D015342:DNA Probes; D004768:Enterotoxins / Q000032:analysis / Q000235:genetics; D005243:Feces / Q000382:microbiology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D014195:Travel
D015342:DNA Probes; D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae