[A metabolic study of the synthetic glucocorticosteroids prednisolone and dexamethasone in the body of white rats and in in-vitro reactions].
The metabolism of the synthetic steroidal hormones prednisolone and dexamethasone was studied in albino rats and in in vitro microsomal oxidation system by using high performance chromatography and radioactive tracers. Dexamethasone was found to be metabolically stable. The first step of prednisolone biotransformation is formation of prednisone during the NADPH-dependent reaction catalyzed by microsomal enzymes. Conditions were created for isolating the ultimate prednisolone metabolite formed in the rats' body by using HPLC.
Maruzhenkov
D S
DS
Krynetskiĭ
E Iu
EIu
Prokhorov
B S
BS
Krylov
Iu F
IuF
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003907:Dexamethasone / Q000493:pharmacokinetics* / Q000652:urine; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D011239:Prednisolone / Q000493:pharmacokinetics* / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D012588:Scintillation Counting; D014316:Tritium
D014316:Tritium; D003907:Dexamethasone; D011239:Prednisolone
Izuchenie metabolizma sinteticheskikh gliukokortikosteroidov prednizolona i deksametazona v organizme belykh krys i v reaktsiiakh in vitro.