[Generation of monoclonal antibodies (McAbs) against human luteinizing hormone (hLH) in a methylcellulose semi-solid medium].
McAbs against hLH were generated by a facile hybridoma technique according to the methods of Davis and Lee, et al. After routine immunization and cell fusion, the cells were plated in a semi-solid medium containing methylcellulose and HAT. Five to seven days later, visible clones were removed for subculture in DMEM liquid medium containing 15% FCS and then screened by ELISA. As a result, 12 hybrid cell lines secreting McAbs to the LH-B subunit were obtained. These Abs have been used in clinical diagnostic and immunohistochemical work, showing good results. The advantages of the semi-solid medium in preparing McAbs are discussed.
Wang
B
B
Institute of Basic Medicine, Beijing.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D003470:Culture Media; D005260:Female; D006825:Hybridomas / Q000378:metabolism; D007986:Luteinizing Hormone / Q000276:immunology*; D008747:Methylcellulose; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007986:Luteinizing Hormone; D008747:Methylcellulose