A short incubation with PGE2 affects the proliferative response of T lymphocytes to PWM.
In this study we have investigated the role of PGE2 in the activation of human T lymphocytes by PWM. A preincubation of these cells with molar concentrations of the prostaglandin ranging from 10(-9) M to 10(-4) M is able to reduce the expression of IL-2R and CD71 on T lymphocyte membrane during the first days of culture, while the DR molecule which is expressed later in the same experimental conditions is not affected by the treatment of T lymphocytes with PGE2. The PGE2-induced inhibition of IL-2R and CD71 well correlates with the reduction of 3H-thymidine incorporation by T cells, indicating that a preincubation of T lymphocytes with PGE2 profoundly affects the proliferative apparatus of these cells when they are stimulated by PWM.
Santarpia
G
G
Institute of General Pathology, University of Messina, Medical School, Italy.
Valenti
A
A
Loteta
L
L
Sofo
V
V
Sottile
A
A
Serrao
N
N
Salmeri
F M
FM
Teti
D
D
D000921:Antibody-Producing Cells / Q000187:drug effects; D015703:Antigens, CD / Q000187:drug effects; D000944:Antigens, Differentiation, B-Lymphocyte / Q000187:drug effects; D015232:Dinoprostone / Q000494:pharmacology*; D006684:HLA-DR Antigens / Q000187:drug effects; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000187:drug effects; D011990:Receptors, Transferrin; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C068126:CD71 antigen; D006684:HLA-DR Antigens; D011043:Pokeweed Mitogens; D015375:Receptors, Interleukin-2; D011990:Receptors, Transferrin; D015232:Dinoprostone