A possible role for nonsense suppression in the synthesis of a human cytomegalovirus 58-kDa virion protein.
A 1.6-kb late mRNA originating from the HindIII R fragment of human cytomegalovirus (HCMV) encodes a 58-kDa virion phosphoprotein. Data presented support the hypothesis that this protein may be synthesized via the translational readthrough of an opal termination codon separating two open reading frames located in tandem. To our knowledge this is the first report of nonsense suppression as a means of regulating gene expression in HCMV.
Lahijani
R S
RS
Department of Microbiology, University of Nevada, Reno 89557.
Otteson
E W
EW
St Jeor
S C
SC
D000595:Amino Acid Sequence; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D016153:Genes, Suppressor; D005815:Genetic Code; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D006023:Glycoproteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins