Centriole modification in human aortic endothelial cells.
The structure of centrioles in endothelial cells of embryonic (22-24 weeks old) and definitive (2, 14-17, and 30-40 years) human aorta in situ and also in aortic endothelial cells dividing in organ and cell cultures (donor age 30-40 years) was studied. It was found that in the endothelial cells from definitive aorta the lengths of mother centrioles vary from 0.5 to 2 microns, whereas the length of daughter centrioles remains constant (0.4-0.5 microns). The distal part of the cylinder of long mother centrioles consists of microtubule doublets. In aorta of donors 30-40 years old in multinucleated cells and in one of 30 single-nucleated cells analyzed, C-shaped long centrioles were seen. These centrioles exhibit a doublet organization along all their length. Mitotic cells in organ and cell culture had a nonequal structure of spindle poles: at one pole, the long mother centriole was seen, while at the other a mother centriole of standard size was found. In such cells of organ culture long centrioles make contact with the remnant of primary cilia until the end of anaphase. In cell culture mitotic cells are also observed containing C-shaped centrioles. In these cells the number of mother centrioles is odd and their number is not equal to the number of daughter centrioles. The possible mechanism for transformation of endothelial centrioles and its role in the control of cell-cycle progression are discussed.
Bystrevskaya
V B
VB
Institute of Experimental Cardiology, Cardiology Research Center, Moscow, Russia.
Lichkun
V V
VV
Krushinsky
A V
AV
Smirnov
V N
VN
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001013:Aorta, Thoracic / Q000166:cytology / Q000196:embryology; D002453:Cell Cycle; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D002502:Centrioles / Q000648:ultrastructure*; D002675:Child, Preschool; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D006801:Humans; D008297:Male; D008938:Mitosis; D008941:Spindle Apparatus / Q000648:ultrastructure