IgM capture ELISA for detection of IgM antibodies to dengue virus: comparison of 2 formats using hemagglutinins and cell culture derived antigens.
The highly sensitive AFRIMS format IgM capture ELISA for the diagnosis of dengue virus infections requires the use of mouse brain derived hemagglutinins and consequently also the use of 20% acetone extracted normal human serum to eliminate high background. These reagents are not always easily available and we have thus compared the AFRIMS format with another published format which uses cell culture derived antigens (culture fluid, CF, format) in order to determine if it is reasonable to use cell culture derived antigens in situations where hemagglutinins and normal human serum are difficult to obtain. The study shows that using AFRIMS results as the reference point, the CF format described here has a sensitivity of 90% and a specificity of 96%.
Cardosa
M J
MJ
School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang.
Tio
P H
PH
Nimmannitya
S
S
Nisalak
A
A
Innis
B
B
D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007075:Immunoglobulin M