[Oxygen affinity and regeneration capacity of SAG-M and PAGGS-M stored erythrocytes].
In order to estimate the leftward shift of the oxygen dissociation curve of hemoglobin following storage of red blood cells (rbc) in the additive solutions SAG-M and PAGGS-M, respectively, we performed blood gas analyses after equilibrating the cells with a gas mixture containing 4% of O2 and 5% of CO2 at pH 7.4 and 37 degrees C. Additionally, we took advantage of these nearly physiological conditions to measure the recovery of the hemoglobin function in vitro. We observed a good correlation between 2,3-BPG and p50 (i.e. the oxygen tension, at which hemoglobin is half-saturated with oxygen). Within the first 3 weeks of storage, the 2,3-BPG content fell to one fifth of its original value, whereas the p50 declined from 26.6 to about 20 mm Hg. Compared to fresh cells, rbc stored for longer than 3 weeks will thus deliver 30% less oxygen to the myocardium. The rbc's ability to restore these parameters remained unchanged throughout 7 weeks of storage.
von Eisenhart-Rothe
B
B
Abteilung für Transfusionsmedizin und Transplantationsimmunologie, Chirurgische Universitätsklinik, Hamburg-Eppendorf, BRD.
Dominka
T
T
Rödiger
W
W
Ganschow
I
I
Ivens
B
B
Reckhaus
M L
ML
Renner
V
V
Wirsig
H W
HW
D019794:2,3-Diphosphoglycerate; D000225:Adenine; D016913:Blood Component Transfusion; D001793:Blood Preservation; D004163:Diphosphoglyceric Acids / Q000097:blood; D004905:Erythrocyte Aging / Q000502:physiology; D005947:Glucose; D006151:Guanosine; D006801:Humans; D008353:Mannitol; D010100:Oxygen / Q000097:blood*; D010710:Phosphates; D012965:Sodium Chloride; D013012:Sorbitol; D013997:Time Factors
C037839:ADSOL; D004163:Diphosphoglyceric Acids; C053620:PAGGS-sorbitol solution; D010710:Phosphates; D006151:Guanosine; D019794:2,3-Diphosphoglycerate; D008353:Mannitol; D012965:Sodium Chloride; D013012:Sorbitol; D005947:Glucose; D000225:Adenine; D010100:Oxygen
Sauerstoffaffinität und Regenerationsfähigkeit in SAG-M und PAGGS-M gelagerter Erythrozyten.