Sensitivity to plating of Escherichia coli cells expressing the cryA gene from Bacillus thuringiensis var. israelensis.
The gene (cytA) coding for the 27 kDa polypeptide of the Bacillus thuringiensis var. israelensis mosquito larvicidal delta-endotoxin, was cloned into a plasmid containing the T7 bacteriophage promoter. The plasmid was used to transform an Escherichia coli strain containing the T7 RNA polymerase gene 1, under the control of lacP. Loss of colony-forming ability without substantial lysis, associated with immediate inhibition of DNA synthesis, was observed after induction of transformed cells. The cytA gene product may kill E. coli cells by disrupting their chromosome replicating apparatus.
Douek
J
J
Department of Life Sciences, Ben-Gurion University of the Negev, Be'er-Sheva, Israel.
Einav
M
M
Zaritsky
A
A
D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D003001:Cloning, Molecular; D015169:Colony Count, Microbial; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004731:Endotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D006460:Hemolysin Proteins; D007763:Lac Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D013604:T-Phages / Q000201:enzymology / Q000235:genetics
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D012321:DNA-Directed RNA Polymerases