Effects of calcitonin and calcitonin gene-related peptide on 3H-thymidine incorporation into DNA of rat thyroid lobes in vitro.
The effects of a 4 h incubation of rat thyroid lobes, in the presence of calcitonin (CT) and calcitonin gene-related peptide (CGRP) on the incorporation of 3H-thymidine into DNA, were investigated. In other groups the thyroid lobes were incubated during exposure to CT and thyrotropin (TSH), and to CGRP together with TSH. All concentrations of CT (10(-6)-10(-8) M) revealed a tendency towards lowering 3H-thymidine uptake, but the effect was not statistically significant. The influence of CGRP was dose-dependent; the lowest concentration of CGRP (10(-9) M) significantly enhanced DNA synthesis in the incubated rat thyroids; an intermediate dose of the peptide (10(-8) M) had no effect, while the highest concentration of CGRP (10(-7) M) decreased 3H-thymidine incorporation. Calcitonin (10(-7) M), as well as CGRP (10(-8) M), suppressed the stimulatory effect of TSH on 3H-thymidine incorporation.
Rybicka
I
I
Laboratory of Thyrology, University School of Medicine, Lódź, Poland.
Lewiński
A
A
Kunert-Radek
J
J
Faryna
M
M
D000818:Animals; D002116:Calcitonin / Q000494:pharmacology*; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002450:Cell Communication; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D013961:Thyroid Gland / Q000187:drug effects*; D013972:Thyrotropin / Q000037:antagonists & inhibitors
D013972:Thyrotropin; D002116:Calcitonin; D004247:DNA; D015740:Calcitonin Gene-Related Peptide; D013936:Thymidine