Effects of the amphiphilic peptides melittin and mastoparan on calcium influx, phosphoinositide breakdown and arachidonic acid release in rat pheochromocytoma PC12 cells.
Two amphiphilic peptides from hymenopterid insects, melittin and mastoparan, stimulate secretion in a variety of cell types. In PC12 cells, both peptides stimulate calcium influx with melittin some 20-fold more potently than mastoparan. Melittin stimulates both breakdown of phosphoinositides (Pl) by phospholipase C to yield inositol phosphates and hydrolysis of phospholipids by phospholipase A2 to release arachidonic acid (AA). Mastoparan stimulates Pl breakdown, but has no effect on AA release. Maximal stimulation of Pl breakdown occurs at 1 to 2.5 micrograms/ml melittin and 30 micrograms/ml mastoparan, whereas maximal stimulation of AA release occurs at 2 to 5 micrograms/ml melittin. Organic calcium channel blockers (nifedipine, verapamil, diltiazem) have little or no effect on responses to the peptides. The influx of calcium elicited by melittin or mastoparan is completely or nearly completely blocked by inorganic calcium channel blockers (Co++, Mn++, Cd++). Mn++ and Cd++ inhibit melittin-induced Pl breakdown and AA release and mastoparan-induced Pl breakdown. Co++ has no effect on melittin-induced Pl breakdown and potentiates mastoparan-induced Pl breakdown. Pertussis toxin has no effect on the Pl breakdown induced by either peptide. The responses to melittin and mastoparan in PC12 cells are compared to those reported for maitotoxin.
Choi
O H
OH
Laboratory of Bioorganic Chemistry, National Institute of Diabetes, Digestive Disease, National Institutes of Health, Bethesda, Maryland.
Padgett
W L
WL
Daly
J W
JW
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism / Q000493:pharmacokinetics*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D008387:Marine Toxins / Q000494:pharmacology; D008555:Melitten / Q000494:pharmacology*; D035964:Oxocins; D016716:PC12 Cells; D010455:Peptides; D037342:Pertussis Toxin; D010716:Phosphatidylinositols / Q000378:metabolism*; D010741:Phospholipases A / Q000187:drug effects / Q000378:metabolism; D054467:Phospholipases A2; D051381:Rats; D010738:Type C Phospholipases / Q000187:drug effects / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D014862:Wasp Venoms / Q000494:pharmacology*; D000095702:Polyether Toxins
D016718:Arachidonic Acid; D002118:Calcium; D002121:Calcium Channel Blockers; D036341:Intercellular Signaling Peptides and Proteins; D008387:Marine Toxins; D008555:Melitten; D035964:Oxocins; D010455:Peptides; D037342:Pertussis Toxin; D010716:Phosphatidylinositols; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases; D010566:Virulence Factors, Bordetella; D014862:Wasp Venoms; C037500:mastoparan; C020394:maitotoxin; D000095702:Polyether Toxins