Antifertility effects in rats actively immunized with 80 kDa human semen glycoprotein.
A 80 kDa human sperm antigen has been identified using the serum of an infertile woman having circulating antisperm antibodies. The antigen was then purified to homogeneity by gel permeation chromatography using HPLC (protein PAK-125 column) system and on FPLC (superose-12 column) system. The antigen was found to be a glycoprotein. The antigen was mainly localized in the postacrosomal region of the human sperm, while it was localized in the head region of the rat sperm as demonstrated by immunofluorescent staining. The presence of this antigen was also demonstrated in the human prostate and endometrium and in the rat testis; epididymis and the prostate by immunocytochemical staining. The purified protein upon active immunization in female rats caused infertility in 100 percent animals. While in male rats it caused infertility in 90 percent animals. On morphometric analysis of testicular tissue it was observed that there was no significant change in spermatogonia and spermatocytes, but significant decrease in spermatids and sperm number as well as daily sperm production in the immunized male rats. The epididymal spermatozoa were markedly reduced in number and were largely found to be agglutinated. The results suggest that 80 kDa human sperm antigen appears to be a suitable candidate for immunocontraception both in male and female.
Bandivdekar
A H
AH
Institute for Research in Reproduction (ICMR), Bombay, India.
Gopalkrishnan
K
K
Garde
S V
SV
Fernandez
P X
PX
Moodbidri
S B
SB
Sheth
A R
AR
Koide
S S
SS
D000818:Animals; D003269:Contraception, Immunologic; D005260:Female; D005298:Fertility / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006801:Humans; D007114:Immunization; D007150:Immunohistochemistry; D008297:Male; D011247:Pregnancy; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis / Q000276:immunology / Q000494:pharmacology*; D051381:Rats; D029589:Seminal Plasma Proteins; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*; D013735:Testicular Hormones / Q000032:analysis / Q000276:immunology / Q000494:pharmacology*
D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; D013735:Testicular Hormones; C043076:beta-microseminoprotein