title: Effect of centrifugation on amniotic fluid phospholipid composition.
abstract: The first step in the determination of phospholipid in amniotic fluid is generally the removal of cells and debris from the fluid by centrifugation. Low-speed centrifugation of the supernatant is reported to have the same phospholipidic profile and L/S, PG/S and PI/S ratios similar to those of the uncentrifuged amniotic fluid sample. With high-speed centrifugation almost all the pulmonary surfactant seems to be recovered in pellet with the same characteristics as the uncentrifuged amniotic fluid.
authors.affiliation: Departamento de Química Orgánica, Facultad de Medicina, Universidad de Zaragoza, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Ruiz-Budría
authors.forename: E
authors.initials: E
authors.lastname: Fabre
authors.forename: M T
authors.initials: MT
authors.lastname: Higueras
authors.forename: J L
authors.initials: JL
authors.lastname: Serrano
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry*; D002498:Centrifugation; D005260:Female; D005325:Fetal Organ Maturity; D006801:Humans; D008168:Lung / Q000196:embryology*; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D011247:Pregnancy; D011663:Pulmonary Surfactants / Q000032:analysis*; D013109:Sphingomyelins / Q000032:analysis
chemical_list: D010713:Phosphatidylcholines; D010743:Phospholipids; D011663:Pulmonary Surfactants; D013109:Sphingomyelins