title: Acid DNase activities in peripheral, mononuclear blood cells: a possible parameter to detect proliferating cell populations.
abstract: After electrophoresis in DNA-containing polyacrylamide gels, two acid DNase activities can be detected in peripheral, mononuclear cells of the human blood. One of these acid DNase activities correlates with cell proliferation; its isoelectrical point is at pI 7.4. By means of this DNase activity, a quantity of less than 1% leukemic cells can be detected. The increased acid DNase activity can indicate the proliferation of malignant cell populations and possibly the proliferation of cell populations during immunological reactions.
authors.affiliation: Abteilung für Physiologische Chemie, Universität Mainz, Bundesrepublik Deutschland.
authors.forename: M
authors.initials: M
authors.lastname: Reitz
authors.forename: P
authors.initials: P
authors.lastname: Gutjahr
mesh_terms: D004247:DNA / Q000378:metabolism; D003851:Deoxyribonucleases / Q000097:blood* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000201:enzymology* / Q000276:immunology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000201:enzymology*; D012016:Reference Values
chemical_list: D007527:Isoenzymes; D004247:DNA; D003851:Deoxyribonucleases