Extrathymic origin of V gamma 1/V delta 6 T cells in the skin.
The dendritic epidermal cells (dEC) from young (8-week-old) athymic mice were found to express mRNA for gamma/delta T cell receptors (TcR). The mRNA found in the dEC from athymic mice was expressed only for V gamma 1-J gamma 4-C gamma 4 and V delta 6-J delta 1-C delta with both chains having productive rearrangements and junctional diversity. On the other hand, the dEC from euthymic mice carried V gamma 5-J gamma 1-C gamma 1 and V delta 1-J delta 2-C delta in addition to the V gamma 1/V delta 6 subset found in athymic mice. Therefore, this V gamma 1/V delta 6 subset, which has been implicated in the murine gamma/delta T cell responses to mycobacterial antigens and self heat-shock proteins, is the product of extrathymic maturation in the dEC from both athymic and euthymic mice.
Ota
Y
Y
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Kobata
T
T
Seki
M
M
Yagita
H
H
Shimada
S
S
Huang
Y Y
YY
Takagaki
Y
Y
Okumura
K
K
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002454:Cell Differentiation; D015870:Gene Expression; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude / Q000276:immunology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology*; D013950:Thymus Gland / Q000166:cytology
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta