title: [BspLS2I--a new site-specific endonuclease from the thermophilic bacteria Bacillus species LS2].
abstract: A new restriction endonuclease BspLS2I was isolated from the thermophilic bacterium Bacillus species LS2 and purified by blue sepharose and hydroxyapatite chromatographies. The enzyme is an isoschizomer of SduI from Streptococcus durans. BspLS2I recognizes the sequence 5' G(G/A/T)GC(C/T/A) decreases C 3' on double-stranded DNA and cleaves it is indicated by the arrow to yield sticky-ended DNA fragments. Maximum catalytic activity of endonuclease was found in 10 mM tris-HCl (pH 7.9) in the presence of 15-30 mM MgCl2 at 50 degrees C. The phage T4 glucosylated DNA is not cleaved by the enzyme.
authors.forename: N P
authors.initials: NP
authors.lastname: Kovalevskaia
authors.forename: L A
authors.initials: LA
authors.lastname: Zheleznaia
authors.forename: N I
authors.initials: NI
authors.lastname: Matvienko
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific; C033083:GDGCHC-specific GATC-specific type II deoxyribonucleases
vernacular_title: BspLS2I--novaia saĭt-spetsificheskaia éndonukleaza iz termofil'noĭ bakterii Bacillus species LS2.