Comparative determinations of low-density-lipoprotein-cholesterol.
Two different methods for low-density-lipoprotein-cholesterol (LDL-C) determination were comparatively used. The heparin-sodium citrate (pH 5.12) precipitation method gave similar LDL-C results to the ones given by the Friedewald et al. formula (3.2 vs 3.3 mmol/l) in 187 men. Values obtained using both methods show a very high and significant correlation (r > 0.9; p < 0.001). However, LDL-C values obtained with the precipitation method were 15% higher in hypertriglyceridemics (triglycerides (Tg) > or = 2.3 mmol/l). A paired-comparison between data obtained by both methods indicates a clear serum Tg-values influence, because LDL-C values obtained by the precipitation method were significantly more frequently higher (p < 0.05 or p < 0.01) than LDL-C values obtained using the Friedewald's formula in hypertriglyceridemic men (Tg > or = 1.7 mmol/l or Tg > or = 2.3 mmol/l respectively). When a 3.9 mmol/l LDL-C level break was chosen, Friedewald's formula gave 13% false hypercholesterolemics. The influence of Tg was again significant in men with both, hypercholesterolemia and hypertriglyceridemia, while LDL-C values obtained by the precipitation method were significantly more frequently higher (p < 0.01).
Sánchez-Muniz
F J
FJ
Departamento de Nutrición y Bromatología I (Nutrición), Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Cuesta
C
C
San-Félix
B
B
D000328:Adult; D000465:Algorithms; D011232:Chemical Precipitation; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood*; D002951:Citrates; D019343:Citric Acid; D006493:Heparin; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D015228:Hypertriglyceridemia / Q000097:blood; D008297:Male; D008875:Middle Aged
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D002951:Citrates; D019343:Citric Acid; D006493:Heparin