Axonal flow blockade induces alpha-CGRP mRNA expression in rat motoneurons.
Axonal flow of motoneurons was blocked in the sciatic nerve by the application of vinblastine at a low concentration (100 microM) not considered likely to cause axonal degeneration. The number of motoneurons expressing alpha-calcitonin gene-related peptide (CGRP) mRNA was significantly increased by vinblastine application, while the expression of beta-CGRP mRNA was not influenced. These findings suggest that the synthesis of these two peptides is differentially regulated and that alpha-CGRP mRNA is possibly down-regulated by unknown factor(s) which normally undergo retrograde transport from the periphery.
Katoh
K
K
Department of Anatomy, Osaka University Medical School, Japan.
Tohyama
M
M
Noguchi
K
K
Senba
E
E
D000818:Animals; D001370:Axonal Transport / Q000187:drug effects; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008297:Male; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology*; D009410:Nerve Degeneration; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000502:physiology*; D014747:Vinblastine / Q000494:pharmacology*
D012333:RNA, Messenger; D014747:Vinblastine; D015740:Calcitonin Gene-Related Peptide