title: [Modification of Harada's method for rapid staining of mycobacteria].
abstract: Harada employed periodic acid-carbol pararosaniline and periodic acid-methenamine silver stain for demonstrating chromophobic bacilli which do not get stained with conventional carbol fuchsin or counter stain. This staining method takes considerable time for complete oxidation with periodic acid. We have succeeded in reducing the oxidation time by using hydrogen peroxide treatment prior to periodic acid and with the use of acidified sodium hydrogen sulfite treatment before carbol pararosaniline stain. We also found that in methenamine silver stain, combined use of semicarbazide and microwave treatment can shorten the whole staining time up to four hours without losing ito sensitivity.
authors.affiliation: National Institute for Leprosy, Tokyo.
authors.forename: K
authors.initials: K
authors.lastname: Kawatsu
authors.forename: S
authors.initials: S
authors.lastname: Izumi
authors.forename: M
authors.initials: M
authors.lastname: Yumi
authors.forename: K I
authors.initials: KI
authors.lastname: Butt
authors.forename: T
authors.initials: T
authors.lastname: Wang
mesh_terms: D000818:Animals; D001135:Armadillos; D006801:Humans; D006861:Hydrogen Peroxide; D051379:Mice; D008819:Mice, Nude; D008872:Microwaves; D009161:Mycobacterium / Q000302:isolation & purification*; D016622:Silver Staining / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
chemical_list: D006861:Hydrogen Peroxide