[Routine application of HLA class II oligotyping. Value of automatization].
HLA class II typing by DNA amplification and hybridization with allele-specific oligonucleotides has led to an improvement of the overall typing accuracy. In an attempt to apply this procedure to a large-scale analysis, we sought to implement an automation system using the Biomek 1,000 robotic work-station. This system allows a rapid preparation of many consecutive samples and avoids the risk of human errors. It is now routinely used in several laboratories.
Caillat-Zucman
S
S
INSERM U 25, Hôpital Necker, Paris.
Bach
J F
JF
D001331:Automation; D005802:Genes, MHC Class II / Q000235:genetics*; D006801:Humans; D016377:Organ Transplantation; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods
Application en routine de l'oligotypage HLA de classe II. Intérêts de l'automatisation.