[Analysis of the electrical charge of albumin in idiopathic nephrotic syndrome by isoelectric focusing].
The state of the electrical charge of serum and urinary albumin was investigated in both the nephrotic and remission stage of idiopathic nephrotic syndrome (INS), using isoelectric focusing (IEF). 1) Both a b2 band (a main albumin band appearing at the site of isoelectric point: pI 4.7), and a b3 band (a more anionic albumin band than the b2 band) were detected commonly in all samples of urine and serum of INS patients in nephrotic and remission stages and of healthy volunteer controls even if the applied albumin in urine and serum amounted to 20 micrograms or 100 micrograms. 2) When applied albumin amounted to 20 micrograms, b1 bands (less anionic albumin bands than the b2 band) were detected between pI 4.7 and pI 6.5 in urine and in both serum and purified albumin fractionated from serum of INS patients in the nephrotic stage. However, b1 bands were not detected at all in the urine and serum of either INS patients in remission stage and healthy volunteer controls. 3) When applied albumin amounted to 100 micrograms, b1 bands were detected also in serum of healthy volunteer controls. From these results, it was confirmed that less anionic albumin existed also in the serum of healthy volunteer controls although the amount was extremely small.(ABSTRACT TRUNCATED AT 250 WORDS)
Yamaguchi
Y
Y
Department of Pediatrics, St. Marianna University School of Medicine, Kanagawa, Japan.
Mio
H
H
Shibawaka
T
T
Ikoma
M
M
Meguro
T
T
Fujita
R
R
Koitabashi
Y
Y
Yamada
K
K
D000418:Albumins / Q000302:isolation & purification* / Q000378:metabolism; D000419:Albuminuria / Q000209:etiology; D001485:Basement Membrane / Q000378:metabolism; D006801:Humans; D007525:Isoelectric Focusing; D007678:Kidney Glomerulus / Q000378:metabolism; D009404:Nephrotic Syndrome / Q000209:etiology / Q000378:metabolism*
D000418:Albumins