A rapid and sensitive method for HPLC cholesterol determination in bile.
A relatively little time consuming simple method based on the treatment of bile with cholesterol oxidase and subsequent high performance liquid chromatography measurement of the 3-ketocholesterol produced in order to determine the level of the cholesterol concentration is described. The method avoids bilirubin interferences, has high reproducibility and recovery assays give 100% values. It is highly sensitive and suitable for use in the determination of cholesterol concentrations in bile and other bilirubin containing biological fluids.
Bocos
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Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, Hospital Ramón y Cajal, Madrid, Spain.
Castro
M
M
Orozco
E
E
Contreras
J A
JA
Herrera
E
E
D000818:Animals; D001646:Bile / Q000737:chemistry*; D002784:Cholesterol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D007653:Ketocholesterols / Q000032:analysis*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D007653:Ketocholesterols; D002784:Cholesterol