A biosensor for L-proline determination by use of immobilized microbial cells.
A biosensor to quantify L-proline within 10(-5)-10(-3) mole/L concentration is described. Immobilized Pseudomonas sp. cells grown in a medium containing L-proline as the only source of carbon and nitrogen were used to create the biosensor. The cells oxidized L-proline specifically consuming O2 and did not react with other amino acids and sugars. The change in oxygen concentration was detected with a Clark oxygen membrane electrode. The cells were immobilized by entrapment in polyvinyl alcohol (PVA) cryogel. The resultant biocatalyst had a high mechanical strength and retained its L-proline-oxidizing ability for at least two months.
Simonian
A L
AL
Yerevan Physics Institute, Armenia.
Rainina
E I
EI
Lozinsky
V I
VI
Badalian
I E
IE
Khachatrian
G E
GE
Tatikian
S Sh
SSh
Makhlis
T A
TA
Varfolomeyev
S D
SD
D000255:Adenosine Triphosphate / Q000032:analysis; D001431:Bacteriological Techniques / Q000295:instrumentation; D015374:Biosensing Techniques; D003470:Culture Media; D010101:Oxygen Consumption; D011392:Proline / Q000032:analysis*; D011549:Pseudomonas / Q000737:chemistry* / Q000254:growth & development
D003470:Culture Media; D000255:Adenosine Triphosphate; D011392:Proline