title: Antibody-capture enzyme-linked immunosorbent assays that use enzyme-labelled antigen for detection of virus-specific immunoglobulin M, A and G in patients with varicella or herpes zoster.
abstract: Antibody-capture enzyme-linked immunosorbent assays (AC-ELISA) which use enzyme-labelled antigen were developed for detection of varicella-zoster virus-(VZV) specific IgM, IgA and IgG antibody in patients with varicella or herpes zoster and in sera from healthy individuals. All 18 patients with varicella developed a VZV-IgM and a VZV-IgG response, 17 also a VZV-IgA response. In contrast, all 19 patients with herpes zoster were shown to be positive for VZV-IgA whereas only 13 of these reacted positively for VZV-IgM. A VZV-IgM response was detected in only two sera from 100 healthy individuals and an IgA response in only one. The presence of virus-specific IgA and IgG in the cerebrospinal fluid as determined by AC-ELISA was a useful indicator of VZV infection of the central nervous system. By AC-ELISA, VZV-IgG was detected predominantly in sera from patients with acute or recent VZV infection. Only 14 sera from 100 healthy individuals were positive for VZV-IgG by AC-ELISA, whereas all were positive by an indirect ELISA. These results indicate that AC-ELISA's may be useful assays for determination for acute or recurrent VZV infection, but are not suitable for determination of past infection with this virus.
authors.affiliation: Department of Medical Microbiology, University of Nijmegen, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: van Loon
authors.forename: J T
authors.initials: JT
authors.lastname: van der Logt
authors.forename: F W
authors.initials: FW
authors.lastname: Heessen
authors.forename: M C
authors.initials: MC
authors.lastname: Heeren
authors.forename: J
authors.initials: J
authors.lastname: Zoll
mesh_terms: D000208:Acute Disease; D000914:Antibodies, Viral / Q000097:blood; D002644:Chickenpox / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005069:Evaluation Studies as Topic; D005500:Follow-Up Studies; D006562:Herpes Zoster / Q000276:immunology*; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000134:cerebrospinal fluid; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid; D007075:Immunoglobulin M / Q000097:blood; D007136:Immunoglobulins / Q000097:blood* / Q000134:cerebrospinal fluid; D008590:Meningoencephalitis / Q000276:immunology; D011237:Predictive Value of Tests; D012008:Recurrence
chemical_list: D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins