title: A survey of cold-growing gram-negative organisms isolated from the skin of prospective blood donors.
abstract: The skin of the antecubital fossae of 90 subjects, chosen at random but eligible to be blood donors, was sampled using sterile swabs. This sampling was done prior to disinfection. The swabs were inoculated into a selective culture medium and 'incubated' at blood bank refrigerator temperature for a total of 6 weeks. The results show that there were a number of cold-growing Gram-negative bacilli (GNB) on the skin tested. One of the GNB carriers found was subjected to frequent testing both before and after skin disinfection. The routine skin disinfection regime for blood donors was used in an attempt to establish its effectiveness. Despite regularly being able to culture Pseudomonas fluorescens prior to swabbing, no swabs taken after disinfection showed growth. This volunteer was subjected to a skin biopsy to establish whether the skin disinfection might be simply superficial. No growth occurred in the medium into which biopsy tissue, after disinfection, was inoculated. We conclude that although GNB may be present on donors' skin, the disinfection procedure, as used by us prior to donation, was effective.
authors.affiliation: Natal Blood Transfusion Service, Durban, Republic of South Africa.
authors.forename: M A
authors.initials: MA
authors.lastname: Stenhouse
authors.forename: L V
authors.initials: LV
authors.lastname: Milner
mesh_terms: D001782:Blood Donors; D003080:Cold Temperature; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D006306:Health Surveys; D006801:Humans; D011551:Pseudomonas fluorescens / Q000302:isolation & purification; D012867:Skin / Q000382:microbiology*