Synergism between Clostridium thermocellum cellulases cloned in Escherichia coli.
We have obtained a synergistic effect during degradation of Avicel and filter paper by Clostridium thermocellum cellulases (two endoglucanases and one cellobiohydrolase) cloned in Escherichia coli. The highest degree of synergism was found at early stages of reaction, during the first 20 h: 2.5 and 2.9 on Avicel and filter paper, respectively. During combined action of all three cellulases the main product is cellobiose.
Tuka
K
K
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.
Zverlov
V V
VV
Velikodvorskaya
G A
GA
D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D013379:Substrate Specificity
D002475:Cellobiose; D002482:Cellulose; D006026:Glycoside Hydrolases; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; D005947:Glucose