title: Comparison of the nonstructural protein, NS3, of tick-borne and insect-borne orbiviruses.
abstract: The complete nucleotide sequence of the smallest RNA segment (segment 10) of Broadhaven (BRD) virus, a tick-borne orbivirus, was determined from a full-length cDNA clone. The genome segment is 702 nucleotides in length and has a coding capacity for two proteins of either 205 or 199 amino acids, having net charges of +16.5 and +17.5, respectively, at neutral pH. Comparison of the sequence of RNA segment 10 of BRD, bluetongue, African horse sickness, and Palyam viruses revealed amino acid homology of 20 to 30% between the four orbiviruses, with one conserved region of 40 to 50% homology which, in segment 10 of BRD virus, is found between residues 26 and 71.
authors.affiliation: NERC Institute of Virology & Environmental Microbiology, Oxford, United Kingdom.
authors.forename: S R
authors.initials: SR
authors.lastname: Moss
authors.forename: L D
authors.initials: LD
authors.lastname: Jones
authors.forename: P A
authors.initials: PA
authors.lastname: Nuttall
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D018163:Orbivirus / Q000235:genetics*; D016415:Sequence Alignment; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics*; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins