Changes of soluble glycoproteins in dystrophic (dy/dy) mouse muscle shown by lectin binding.
Lectin binding sites in skeletal muscle from normal and dystrophic (dy/dy) C57 BL/6J mice were demonstrated by use of histochemistry and electrophoresis combined with electron microscopy. The following lectins were used: Canavalia ensiformis Con A, Triticum vulgaris (WGA), Glycine max (SBA), Griffonia simplicifolia (GS II), Arachis hypogaea (PNA), Pisum sativum (PSA) and Lens culinaris (LCA). After incubation of frozen sections with Con A, WGA, GS II, PSA and LCA a sarcoplasmic staining was observed in both normal and dystrophic muscle. The most consistent light microscopic observations in the dystrophic muscles were a decreased staining intensity of the sarcoplasm after incubation with Con A, WGA, PSA and LCA, but not with GS II, and a strong staining of the interfiber connective tissue. Supernatants, deprived of organelles and membranes, were prepared from normal and dystrophic muscle by high speed centrifugation. Lectin stained Western blots of the supernatant from dystrophic muscle showed two bands (120 and 67 K) with high affinities to avidin. Further this supernatant contained two glycoprotein bands (180 and 140 K) with affinities to Con A and a number of glycoprotein bands with apparent molecular weights below 67 K showing affinities to LCA and PSA. None of these glycoprotein bands could be detected in the supernatant from normal muscle. These changes of the muscle carbohydrate components might be involved in the expression of the dystrophic syndrome This seems to be the first report on changes of soluble glycoproteins in muscular dystrophy.
Kirkeby
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S
Institute of Anatomy, Royal Dental College of Copenhagen, Denmark.
Garbarsch
C
C
Matthiessen
M E
ME
Bøg-Hansen
T C
TC
Moe
D
D
D000818:Animals; D015153:Blotting, Western; D003208:Concanavalin A / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism*; D006651:Histocytochemistry; D037102:Lectins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D009132:Muscles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D009137:Muscular Dystrophy, Animal / Q000378:metabolism* / Q000473:pathology; D015388:Organelles / Q000648:ultrastructure; D019887:Peanut Agglutinin; D037121:Plant Lectins; D012995:Solubility; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D006023:Glycoproteins; C045475:Griffonia simplicifolia lectins; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins; C036292:pea lectin; D003208:Concanavalin A