Analysis of human herpesvirus 6 glycoproteins recognized by monoclonal antibody OHV1.
A virus-specific glycoprotein (gp) from human herpes-virus 6 (HHV-6) was studied using the anti-HHV-6 monoclonal antibody OHV1. Immunoprecipitation with extracts from infected cells revealed that the antibody recognized four glycosylated proteins (gps) with Mrs of 106K, 102K, 65K and 63K under reducing conditions. However, only two gps, of 106K (gp106) and 102K, were detected under non-reducing conditions. Pulse-chase experiments revealed that gp65 and gp63 were cleavage products of gp106 and gp102. When infected cells were treated with tunicamycin, none of these gps was detected. With endo-beta-N-acetylglucosaminidase H (endo H) and endo-beta-N-acetylglucosaminidase F (endo F) treatment, gp106 and gp102 disappeared. Moreover, gp65 and gp63 were not affected by endo H treatment but were sensitive to endo F treatment. These data suggest that sugar residues of gp106 and gp102 are high-mannose type N-linked oligosaccharides, whereas those of gp65 and gp63 are complex type N-linked oligosaccharides.
Okuno
T
T
Department of Virology, Osaka University, Japan.
Shao
H
H
Asada
H
H
Shiraki
K
K
Takahashi
M
M
Yamanishi
K
K
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005312:Fetal Blood / Q000166:cytology; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D015654:Herpesvirus 6, Human / Q000737:chemistry* / Q000276:immunology; D006596:Hexosaminidases / Q000494:pharmacology; D006801:Humans; D006825:Hybridomas; D008214:Lymphocytes / Q000382:microbiology; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D009844:Oligosaccharides / Q000032:analysis; D011233:Precipitin Tests; D014415:Tunicamycin / Q000494:pharmacology; D014764:Viral Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D009844:Oligosaccharides; D014764:Viral Proteins; D014415:Tunicamycin; D006596:Hexosaminidases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase