[Effects of platelet activating factor on the cerebrovascular permeability in rats and the protection by drug].
The action of platelet activating factor in the rat brain was detected by Evans blue staining after injection of PAF into the rat brain. The results show that PAF increased the Evans blue staining of the brain, but no staining was observed without prior injection of PAF. Meanwhile, PAF was shown to stimulate the release of 14C-arachidonic acid (14C-AA) in the cerebral microvascular smooth muscle cells (CMSMC). SZ-1, a new synthetic drug, dose-dependently inhibited the Evans blue staining of the rat brain and the PAF induced 14C-AA release in CMSMC. These results indicate that the action of PAF in the rat brain might be related to the stimulation of AA release. SZ-1 may antagonize the PAF receptor and protect the brain from PAF induced damage.
Sun
D X
DX
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai.
Huang
T G
TG
Zeng
G Q
GQ
Zhu
J
J
Ju
D W
DW
Rui
Y C
YC
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D001921:Brain / Q000098:blood supply*; D002199:Capillary Permeability / Q000187:drug effects*; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D011714:Pyrans / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D010972:Platelet Activating Factor; D011714:Pyrans; C079963:1,5-bis(3,4-dimethoxyphenyl)tetrahydropyran; D016718:Arachidonic Acid