Effect of human growth hormone on the proliferation of human fetal islet cells in vitro.
In this study it was shown that 1000 micrograms/L hGH in serum-free medium promoted the attachment, spreading and formation of monolayer of islet cells. The radioactivity test indicated that hGH significantly stimulated the DNA synthesis of islet cells (P < 0.001). Furthermore, after 48 h exposure to hGH (1000 micrograms/L), both the insulin contents and release of islet cells significantly increased (P < 0.001); hGH also enhanced the responsiveness of fetal islet cells to high-concentration glucose plus theophylline stimulation (P < 0.001). However, the effect of hGH in the medium containing 10% newborn calf serum was not so prominent in comparison with that in the serum-free medium. The morphologic assay for mitotic cells showed a combination of 1000 micrograms/L hGH and 10% serum was the most efficient for inducing the mitosis, the mitotic index being 5.95%. We conclude that hGH is an important growth factor for human islet cells.
Hu
Y F
YF
Diabetes Research Laboratory, Shanghai First People's Hospital, Shanghai Medical University.
Ding
Y M
YM
Wang
Y F
YF
Zhang
H
H
Gu
Z F
ZF
Zhang
H D
HD
Zhu
Y L
YL
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D005333:Fetus; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects*
D007328:Insulin; D013006:Growth Hormone