In vitro evaluation of plasticizer activity on the growth and metabolism of chick embryo fibroblasts and on the development of chick embryo lungs.
Administration of di(2-ethylhexyl) phthalate (DEHP) to primary cultures of chick embryo fibroblasts brought about a decrease in cell proliferation rate after 48 h and an inhibition of both DNA and protein synthesis measured by [3H]thymidine and [3H]leucine, respectively, after 48 h. The growth of chick embryo lung rudiments in vitro was also depressed by DEHP treatment. Lung rudiment were smaller in DEHP-treated embryos after 6 days' treatment. These results indicate that DEHP has a cytostatic effect on embryonic cells and tissues.
Stabellini
G
G
Institute of Histology and Embryology, University of Ferrara, Italy.
Fiocchi
O
O
Pellati
A
A
Caruso
A
A
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004051:Diethylhexyl Phthalate / Q000008:administration & dosage / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000254:growth & development*; D009924:Organ Culture Techniques
D004051:Diethylhexyl Phthalate