Replication and persistence of HPV DNA in cultured cells derived from laryngeal papillomas.
We have investigated the replication and persistence of human papillomavirus (HPV) type 6 and 11 DNA in cultured cells derived from laryngeal papillomas, with paradoxical findings. Measured by bromodeoxyuridine incorporation into heavy/light DNA separated on a cesium chloride gradient, viral DNA replicates in both primary and secondary cells. The ratio of the fraction of replicated viral to replicated cellular DNA was equal to or greater than 1 in all but one case and was closer to 2 in primary cells. Despite this efficient replication, HPV DNA is rapidly lost from the cells with passage. We propose that infected cells, or those with a high HPV copy number, show a selective decrease in plating efficiency compared to uninfected cells or those with a low copy number, which explains the loss of HPV DNA with repeated passage.
DiLorenzo
T P
TP
Department of Otolaryngology, Long Island Jewish Medical Center, Albert Einstein College of Medicine, New Hyde Park, New York 11042.
Taichman
L B
LB
Steinberg
B M
BM
D015139:Blotting, Southern; D002448:Cell Adhesion; D002478:Cells, Cultured; D004279:DNA, Viral / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007822:Laryngeal Neoplasms / Q000382:microbiology* / Q000473:pathology; D010212:Papilloma / Q000382:microbiology* / Q000473:pathology; D027383:Papillomaviridae / Q000254:growth & development*; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000382:microbiology*; D014779:Virus Replication
D004279:DNA, Viral