Refined conditions for selective modifications of rabbit skeletal myosin light chains.
We selectively modified the LC1 and LC2 N-terminus as an approach to understand the function of skeletal myosin light chains and their possible implication in some diseases. Three new myosin isoforms were thus created, namely: myosin-[(P)LC1'], myosin-[(T)LC2'] and myosin-[(CT)LC2"] in which the N-terminus was selectively cleaved at Lys7 in (P)LC1', Arg8 in (T)LC2' and Phe19 in (CT)LC2". In order to obtain species with a minimum amount of secondary cleavages, eight to 12 different conditions were screened for each species and the two most efficient conditions were tested at the preparative scale.
Burgat
J M
JM
Laboratoire de Biologie Physico-chimique, Université de Paris-Sud, Orsay, France.
Roulet
A
A
Cardinaud
R
R
D000818:Animals; D001120:Arginine / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008239:Lysine / Q000737:chemistry; D018994:Myosin Light Chains; D009218:Myosins / Q000737:chemistry* / Q000378:metabolism; D010206:Papain; D011817:Rabbits; D014357:Trypsin
D018994:Myosin Light Chains; C076659:myosin light chain I; D001120:Arginine; D014357:Trypsin; D010206:Papain; D009218:Myosins; D008239:Lysine