title: Immunosuppressive effects induced by the polysaccharide moiety of some bacterial lipopolysaccharides.
abstract: The immunomodulatory properties of several lipopolysaccharides (LPS) derived from clinical isolates of Pseudomonas aeruginosa, Branhamella catarrhalis, and Bordetella pertussis were evaluated for their capacity to influence the magnitude of the antibody response to type III pneumococcal polysaccharide (SSS-III), which is known to be regulated by suppressor and amplifier T cells (Ts and Ta, respectively). The administration of LPS, two days after immunization resulted in a significant increase in the antibody response. Such enhancement may be due mainly to the ability of the lipid A moiety of LPS to abolish the negative effects of activated Ts, thereby enabling Ta function to be more fully expressed; however, B cell mitogenicity of the LPS molecule also may be involved. By contrast, treatment with LPS at the time of immunization with SSS-III induces significant suppression of the SSS-III-specific antibody response; such suppression is not induced by LPS or lipid A derived from Escherichia coli and Salmonella minnesota, and is independent of the capacity of LPS to activate B cells polyclonally, an activity generally attributed to the lipid A fraction of LPS. Studies conducted with the LPS of P. aeruginosa indicated that the suppression induced is T cell dependent and mediated by the polysaccharide (PS) fraction of LPS; it appears to be due-at least in part-to the capacity of PS to expand or increase the size of the precursor pool of Ts, activated in response to SSS-III. The significance of these findings to the pathogenesis of certain gram-negative infections is discussed.
authors.affiliation: Vaccine Department, Statens Seruminstitut, Copenhagen, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Hasløv
authors.forename: A
authors.initials: A
authors.lastname: Fomsgaard
authors.forename: K
authors.initials: K
authors.lastname: Takayama
authors.forename: J S
authors.initials: JS
authors.lastname: Fomsgaard
authors.forename: P
authors.initials: P
authors.lastname: Ibsen
authors.forename: M B
authors.initials: MB
authors.lastname: Fauntleroy
authors.forename: P W
authors.initials: PW
authors.lastname: Stashak
authors.forename: C E
authors.initials: CE
authors.lastname: Taylor
authors.forename: P J
authors.initials: PJ
authors.lastname: Baker
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology; D005260:Female; D007108:Immune Tolerance; D008050:Lipid A / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008819:Mice, Nude / Q000276:immunology; D001936:Moraxella catarrhalis / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D008050:Lipid A; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; C026604:pneumococcal polysaccharide, type III