Structural analysis of the carbohydrate chains isolated from mistletoe (Viscum album) lectin I.
Two glycopeptide fractions prepared from mistletoe (Viscum album) lectin I by Pronase digestion were fractioned by affinity chromatography on a concanavalin A-Sepharose column. With 400-MHz 1H NMR spectroscopy, in conjunction with sugar analysis, the following oligosaccharide structures could be determined: two oligomannose-type glycans in the ratio 4:1, one containing six mannose and the other containing five mannose units, both with two 2-acetamido-2-deoxyglucose units. In addition, a mannotriosyl-->N,N'-diacetylchitobiose glycan containing a xylosyl group and an alpha-fucosyl group (1-->3)-linked to the 2-acetamido-2-deoxyglycosyl-1 residue, a common core element of many plant glycoproteins, was also observed.
Debray
H
H
Laboratoire de Chimie Biologique, Unité Mixte de Recherche du CNRS No. 111, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Wieruszeski
J M
JM
Strecker
G
G
Franz
H
H
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D006020:Glycopeptides / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D014784:Mistletoe; D008969:Molecular Sequence Data; D028321:Plant Preparations; D010940:Plant Proteins; D010946:Plants, Medicinal; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000032:analysis*
D002241:Carbohydrates; D006020:Glycopeptides; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum