Mapping T-cell receptor-peptide contacts by variant peptide immunization of single-chain transgenics.
To test models of T-cell recognition, mice transgenic for T-cell receptor alpha or beta chain have been immunized with variant peptides that force changes in the resulting T-cell response. In particular, charge substitutions on the peptide often elicit reciprocal charges in the junctional (CDR3) sequences of T-cell receptor V alpha or V beta chains, indicating direct T-cell receptor-peptide contact, and allowing derivation of a topology for the T-cell receptor-MHC interaction. At one position on the peptide, variants transformed a homogeneous V beta response into a very heterogeneous one.
Jorgensen
J L
JL
Department of Microbiology and Immunology, Stanford University, California 94305.
Esser
U
U
Fazekas de St Groth
B
B
Reay
P A
PA
Davis
M M
MM
D000595:Amino Acid Sequence; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D001667:Binding, Competitive; D017252:CD3 Complex; D010856:Columbidae; D003574:Cytochrome c Group / Q000276:immunology* / Q000378:metabolism; D014644:Genetic Variation; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D046911:Macromolecular Substances; D008285:Major Histocompatibility Complex; D051379:Mice; D008822:Mice, Transgenic; D008961:Models, Structural; D008969:Molecular Sequence Data; D009036:Moths; D009838:Oligodeoxyribonucleotides; D011487:Protein Conformation; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D003574:Cytochrome c Group; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D011948:Receptors, Antigen, T-Cell