A simple and rapid method for toxicological screening of 25 antidepressants in blood or urine using high performance liquid chromatography with diode-array detection.
A high performance liquid chromatographic method with diode-array detection (HPLC/DAD) for simultaneous screening of 25 antidepressants is presented. After single-step liquid-liquid extraction at pH9.5 using chloroform/2-propanol/n-heptane (60/14/26, v/v), the substances are eluted on a Novapak C18 4-microns column (300 x 3.9 mm, id), with methanol/tetrahydrofuran/pH 2.6 phosphate buffer (65/5/30, v/v) as the mobile phase (flow rate 0.8 ml/min). Full UV spectra from 200 to 400 nm are recorded on-line during the entire analysis and may be automatically compared to spectra stored in a library. The analysis is performed in 14 min. The method is simple, rapid, and highly specific.
Tracqui
A
A
Institut de Médecine Légale, Faculté de Médecine de Strasbourg, France.
Kintz
P
P
Kreissig
P
P
Mangin
P
P
D000928:Antidepressive Agents / Q000097:blood* / Q000145:classification / Q000506:poisoning / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011041:Poisoning / Q000097:blood / Q000652:urine; D013056:Spectrophotometry, Ultraviolet
D000928:Antidepressive Agents